LOCUS       DAEGIQ010000001        84536 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000001 DAEGIQ010000000
VERSION     DAEGIQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.001"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            232..603
                     /locus_tag="KMT45_000001"
     CDS             232..603
                     /locus_tag="KMT45_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBE7003495.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(877..1569)
                     /locus_tag="KMT45_000002"
     CDS             complement(877..1569)
                     /locus_tag="KMT45_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBE7003496.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1572..2357)
                     /locus_tag="KMT45_000003"
     CDS             complement(1572..2357)
                     /locus_tag="KMT45_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE7003497.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2594..3994
                     /gene="lacG"
                     /locus_tag="KMT45_000004"
     CDS             2594..3994
                     /gene="lacG"
                     /locus_tag="KMT45_000004"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBE7003498.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4019..4336
                     /locus_tag="KMT45_000005"
     CDS             4019..4336
                     /locus_tag="KMT45_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBE7003499.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4349..6022
                     /locus_tag="KMT45_000006"
     CDS             4349..6022
                     /locus_tag="KMT45_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBE7003500.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6036..7667
                     /locus_tag="KMT45_000007"
     CDS             6036..7667
                     /locus_tag="KMT45_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBE7003501.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8158..9723)
                     /gene="guaA"
                     /locus_tag="KMT45_000008"
     CDS             complement(8158..9723)
                     /gene="guaA"
                     /locus_tag="KMT45_000008"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBE7003502.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9782..10705)
                     /locus_tag="KMT45_000009"
     CDS             complement(9782..10705)
                     /locus_tag="KMT45_000009"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBE7003503.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10901..11506
                     /locus_tag="KMT45_000010"
     CDS             10901..11506
                     /locus_tag="KMT45_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE7003504.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11814..12830
                     /locus_tag="KMT45_000011"
     CDS             11814..12830
                     /locus_tag="KMT45_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBE7003505.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12971..14272
                     /locus_tag="KMT45_000012"
     CDS             12971..14272
                     /locus_tag="KMT45_000012"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBE7003506.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14318..14980
                     /gene="deoC"
                     /locus_tag="KMT45_000013"
     CDS             14318..14980
                     /gene="deoC"
                     /locus_tag="KMT45_000013"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBE7003507.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15001..15399
                     /locus_tag="KMT45_000014"
     CDS             15001..15399
                     /locus_tag="KMT45_000014"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBE7003508.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15465..16547
                     /locus_tag="KMT45_000015"
     CDS             15465..16547
                     /locus_tag="KMT45_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE7003509.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16759..18321
                     /locus_tag="KMT45_000016"
     CDS             16759..18321
                     /locus_tag="KMT45_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7003510.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18314..19447
                     /locus_tag="KMT45_000017"
     CDS             18314..19447
                     /locus_tag="KMT45_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7003511.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19447..20403
                     /locus_tag="KMT45_000018"
     CDS             19447..20403
                     /locus_tag="KMT45_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7003512.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20502..21668
                     /gene="deoB"
                     /locus_tag="KMT45_000019"
     CDS             20502..21668
                     /gene="deoB"
                     /locus_tag="KMT45_000019"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBE7003513.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21692..22510
                     /locus_tag="KMT45_000020"
     CDS             21692..22510
                     /locus_tag="KMT45_000020"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBE7003514.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22527..23234
                     /gene="deoD"
                     /locus_tag="KMT45_000021"
     CDS             22527..23234
                     /gene="deoD"
                     /locus_tag="KMT45_000021"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBE7003515.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23532..24218)
                     /gene="gpmA"
                     /locus_tag="KMT45_000022"
     CDS             complement(23532..24218)
                     /gene="gpmA"
                     /locus_tag="KMT45_000022"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBE7003516.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24390..24971)
                     /locus_tag="KMT45_000023"
     CDS             complement(24390..24971)
                     /locus_tag="KMT45_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7003517.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25104..25787)
                     /gene="rpiA"
                     /locus_tag="KMT45_000024"
     CDS             complement(25104..25787)
                     /gene="rpiA"
                     /locus_tag="KMT45_000024"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBE7003518.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26106..26519
                     /gene="rpsL"
                     /locus_tag="KMT45_000025"
     CDS             26106..26519
                     /gene="rpsL"
                     /locus_tag="KMT45_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBE7003519.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26603..27073
                     /gene="rpsG"
                     /locus_tag="KMT45_000026"
     CDS             26603..27073
                     /gene="rpsG"
                     /locus_tag="KMT45_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBE7003520.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27167..29254
                     /gene="fusA"
                     /locus_tag="KMT45_000027"
     CDS             27167..29254
                     /gene="fusA"
                     /locus_tag="KMT45_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBE7003521.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29426..30613
                     /gene="tuf"
                     /locus_tag="KMT45_000028"
     CDS             29426..30613
                     /gene="tuf"
                     /locus_tag="KMT45_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE7003522.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31020..31328
                     /gene="rpsJ"
                     /locus_tag="KMT45_000029"
     CDS             31020..31328
                     /gene="rpsJ"
                     /locus_tag="KMT45_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBE7003523.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31359..31988
                     /gene="rplC"
                     /locus_tag="KMT45_000030"
     CDS             31359..31988
                     /gene="rplC"
                     /locus_tag="KMT45_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBE7003524.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32016..32639
                     /gene="rplD"
                     /locus_tag="KMT45_000031"
     CDS             32016..32639
                     /gene="rplD"
                     /locus_tag="KMT45_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBE7003525.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32639..32929
                     /gene="rplW"
                     /locus_tag="KMT45_000032"
     CDS             32639..32929
                     /gene="rplW"
                     /locus_tag="KMT45_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBE7003526.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32968..33801
                     /gene="rplB"
                     /locus_tag="KMT45_000033"
     CDS             32968..33801
                     /gene="rplB"
                     /locus_tag="KMT45_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBE7003527.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33844..34122
                     /gene="rpsS"
                     /locus_tag="KMT45_000034"
     CDS             33844..34122
                     /gene="rpsS"
                     /locus_tag="KMT45_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBE7003528.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34145..34492
                     /gene="rplV"
                     /locus_tag="KMT45_000035"
     CDS             34145..34492
                     /gene="rplV"
                     /locus_tag="KMT45_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBE7003529.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34506..35162
                     /gene="rpsC"
                     /locus_tag="KMT45_000036"
     CDS             34506..35162
                     /gene="rpsC"
                     /locus_tag="KMT45_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBE7003530.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35165..35599
                     /gene="rplP"
                     /locus_tag="KMT45_000037"
     CDS             35165..35599
                     /gene="rplP"
                     /locus_tag="KMT45_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBE7003531.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35589..35777
                     /gene="rpmC"
                     /locus_tag="KMT45_000038"
     CDS             35589..35777
                     /gene="rpmC"
                     /locus_tag="KMT45_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBE7003532.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35802..36068
                     /gene="rpsQ"
                     /locus_tag="KMT45_000039"
     CDS             35802..36068
                     /gene="rpsQ"
                     /locus_tag="KMT45_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBE7003533.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36126..36494
                     /gene="rplN"
                     /locus_tag="KMT45_000040"
     CDS             36126..36494
                     /gene="rplN"
                     /locus_tag="KMT45_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBE7003534.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36531..36839
                     /gene="rplX"
                     /locus_tag="KMT45_000041"
     CDS             36531..36839
                     /gene="rplX"
                     /locus_tag="KMT45_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBE7003535.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36867..37406
                     /gene="rplE"
                     /locus_tag="KMT45_000042"
     CDS             36867..37406
                     /gene="rplE"
                     /locus_tag="KMT45_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBE7003536.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37425..37610
                     /locus_tag="KMT45_000043"
     CDS             37425..37610
                     /locus_tag="KMT45_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBE7003537.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37646..38044
                     /gene="rpsH"
                     /locus_tag="KMT45_000044"
     CDS             37646..38044
                     /gene="rpsH"
                     /locus_tag="KMT45_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBE7003538.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38076..38612
                     /gene="rplF"
                     /locus_tag="KMT45_000045"
     CDS             38076..38612
                     /gene="rplF"
                     /locus_tag="KMT45_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBE7003539.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38773..39129
                     /gene="rplR"
                     /locus_tag="KMT45_000046"
     CDS             38773..39129
                     /gene="rplR"
                     /locus_tag="KMT45_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBE7003540.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39150..39650
                     /gene="rpsE"
                     /locus_tag="KMT45_000047"
     CDS             39150..39650
                     /gene="rpsE"
                     /locus_tag="KMT45_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBE7003541.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39665..39844
                     /gene="rpmD"
                     /locus_tag="KMT45_000048"
     CDS             39665..39844
                     /gene="rpmD"
                     /locus_tag="KMT45_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBE7003542.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39888..40328
                     /gene="rplO"
                     /locus_tag="KMT45_000049"
     CDS             39888..40328
                     /gene="rplO"
                     /locus_tag="KMT45_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBE7003543.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40328..41623
                     /gene="secY"
                     /locus_tag="KMT45_000050"
     CDS             40328..41623
                     /gene="secY"
                     /locus_tag="KMT45_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBE7003544.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41685..42332
                     /locus_tag="KMT45_000051"
     CDS             41685..42332
                     /locus_tag="KMT45_000051"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBE7003545.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42523..42741
                     /gene="infA"
                     /locus_tag="KMT45_000052"
     CDS             42523..42741
                     /gene="infA"
                     /locus_tag="KMT45_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBE7003546.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42774..42890
                     /gene="rpmJ"
                     /locus_tag="KMT45_000053"
     CDS             42774..42890
                     /gene="rpmJ"
                     /locus_tag="KMT45_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBE7003547.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42908..43273
                     /gene="rpsM"
                     /locus_tag="KMT45_000054"
     CDS             42908..43273
                     /gene="rpsM"
                     /locus_tag="KMT45_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBE7003548.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43301..43690
                     /gene="rpsK"
                     /locus_tag="KMT45_000055"
     CDS             43301..43690
                     /gene="rpsK"
                     /locus_tag="KMT45_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBE7003549.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43770..44708
                     /locus_tag="KMT45_000056"
     CDS             43770..44708
                     /locus_tag="KMT45_000056"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBE7003550.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44736..45116
                     /gene="rplQ"
                     /locus_tag="KMT45_000057"
     CDS             44736..45116
                     /gene="rplQ"
                     /locus_tag="KMT45_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBE7003551.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45388..46227
                     /locus_tag="KMT45_000058"
     CDS             45388..46227
                     /locus_tag="KMT45_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBE7003552.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46203..47072
                     /locus_tag="KMT45_000059"
     CDS             46203..47072
                     /locus_tag="KMT45_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBE7003553.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47062..47859
                     /locus_tag="KMT45_000060"
     CDS             47062..47859
                     /locus_tag="KMT45_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBE7003554.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48062..48808
                     /gene="truA"
                     /locus_tag="KMT45_000061"
     CDS             48062..48808
                     /gene="truA"
                     /locus_tag="KMT45_000061"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBE7003555.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49263..51932)
                     /locus_tag="KMT45_000062"
     CDS             complement(49263..51932)
                     /locus_tag="KMT45_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBE7003556.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52170..52358)
                     /locus_tag="KMT45_000063"
     CDS             complement(52170..52358)
                     /locus_tag="KMT45_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBE7003557.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52628..52990
                     /locus_tag="KMT45_000064"
     CDS             52628..52990
                     /locus_tag="KMT45_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBE7003558.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53042..54724
                     /locus_tag="KMT45_000065"
     CDS             53042..54724
                     /locus_tag="KMT45_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBE7003559.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54841..56877
                     /gene="recG"
                     /locus_tag="KMT45_000066"
     CDS             54841..56877
                     /gene="recG"
                     /locus_tag="KMT45_000066"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBE7003560.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            56926..57927
                     /gene="plsX"
                     /locus_tag="KMT45_000067"
     CDS             56926..57927
                     /gene="plsX"
                     /locus_tag="KMT45_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBE7003561.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58049..58291
                     /gene="acpP"
                     /locus_tag="KMT45_000068"
     CDS             58049..58291
                     /gene="acpP"
                     /locus_tag="KMT45_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBE7003562.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58559..59563
                     /locus_tag="KMT45_000069"
     CDS             58559..59563
                     /locus_tag="KMT45_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7003563.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59564..60508
                     /locus_tag="KMT45_000070"
     CDS             59564..60508
                     /locus_tag="KMT45_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7003564.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60508..61470
                     /locus_tag="KMT45_000071"
     CDS             60508..61470
                     /locus_tag="KMT45_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7003565.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61497..62411
                     /locus_tag="KMT45_000072"
     CDS             61497..62411
                     /locus_tag="KMT45_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7003566.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62437..64218
                     /locus_tag="KMT45_000073"
     CDS             62437..64218
                     /locus_tag="KMT45_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE7003567.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64566..65252
                     /locus_tag="KMT45_000074"
     CDS             64566..65252
                     /locus_tag="KMT45_000074"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBE7003568.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65272..68853
                     /gene="smc"
                     /locus_tag="KMT45_000075"
     CDS             65272..68853
                     /gene="smc"
                     /locus_tag="KMT45_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBE7003569.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            68862..69671
                     /locus_tag="KMT45_000076"
     CDS             68862..69671
                     /locus_tag="KMT45_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBE7003570.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69682..70680
                     /gene="ftsY"
                     /locus_tag="KMT45_000077"
     CDS             69682..70680
                     /gene="ftsY"
                     /locus_tag="KMT45_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBE7003571.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            70867..73134
                     /gene="gshAB"
                     /locus_tag="KMT45_000078"
     CDS             70867..73134
                     /gene="gshAB"
                     /locus_tag="KMT45_000078"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBE7003572.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73223..73675)
                     /locus_tag="KMT45_000079"
     CDS             complement(73223..73675)
                     /locus_tag="KMT45_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBE7003573.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            73825..74775
                     /locus_tag="KMT45_000080"
     CDS             73825..74775
                     /locus_tag="KMT45_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBE7003574.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74768..75727
                     /locus_tag="KMT45_000081"
     CDS             74768..75727
                     /locus_tag="KMT45_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBE7003575.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75724..76479
                     /locus_tag="KMT45_000082"
     CDS             75724..76479
                     /locus_tag="KMT45_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7003576.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76502..77455
                     /locus_tag="KMT45_000083"
     CDS             76502..77455
                     /locus_tag="KMT45_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE7003577.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(77978..78274)
                     /locus_tag="KMT45_000084"
     CDS             complement(77978..78274)
                     /locus_tag="KMT45_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBE7003578.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            78629..78985
                     /locus_tag="KMT45_000085"
     CDS             78629..78985
                     /locus_tag="KMT45_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBE7003579.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            78995..79894
                     /locus_tag="KMT45_000086"
     CDS             78995..79894
                     /locus_tag="KMT45_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBE7003580.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            80410..81141
                     /locus_tag="KMT45_000087"
     CDS             80410..81141
                     /locus_tag="KMT45_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBE7003581.1"
                     /translation="MMKKEIVLTAAIMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            81286..83313
                     /locus_tag="KMT45_000088"
     CDS             81286..83313
                     /locus_tag="KMT45_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBE7003582.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            83421..84518
                     /locus_tag="KMT45_000089"
     CDS             83421..84518
                     /locus_tag="KMT45_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBE7003583.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT        27363 a        15187 c        18305 g        23681 t
ORIGIN      
        1 ttcttctaga gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg
       61 atttatatag gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta
      121 atagatttaa taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac
      181 cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat aatgagagat
      241 tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct
      301 gataaggcga aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat
      361 atgagtggtg aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt
      421 atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa
      481 ttaattaata tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg
      541 ttgtttacag ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt
      601 taaccattac gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa
      661 gattggtttg agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga
      721 acgcgtcata tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg
      781 atatagcagt gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca
      841 tttcagttgc ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga
      901 caggtgctct gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc
      961 ctgttcaaat actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc
     1021 cgcacgtttg gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg
     1081 agctaagctc agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa
     1141 agagccgtcg tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc
     1201 aaaataaaaa gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg
     1261 gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt
     1321 ttgcaagcaa agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga
     1381 ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat
     1441 attttttaaa atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag
     1501 ccaagcagct tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat
     1561 ccctttcata gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg
     1621 acatattgtg catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga
     1681 tcgaaatcat gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca
     1741 acgagtagat atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg
     1801 gtactgtaag tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta
     1861 aactttagtc ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc
     1921 cggaaatcac cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa
     1981 actggataat tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt
     2041 tcgattgttg tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct
     2101 gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt
     2161 tcttgatcta aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc
     2221 gatttttcta attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg
     2281 atgatttcat taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat
     2341 ctttcttttt tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga
     2401 caacagtcaa tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca
     2461 atagatttta ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa
     2521 tgttttggat gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg
     2581 aggagagatg tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc
     2641 ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt
     2701 tttagaggaa caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc
     2761 aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg
     2821 gagccgcatt tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca
     2881 tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt
     2941 tgatacccct aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc
     3001 atttgtttcc tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac
     3061 ctttaatgaa atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg
     3121 aatcaaatat gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc
     3181 ccgtgtagtg aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc
     3241 attagagacg aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga
     3301 cgatgcattg tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga
     3361 aaccttgact gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga
     3421 agaagatttc gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca
     3481 ctaccaatgc cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac
     3541 gggtgaaaaa gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga
     3601 aggaattcct cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt
     3661 gctgcgaatt aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat
     3721 ggggtataaa gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt
     3781 gagagtctat ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta
     3841 ttttttgtgg tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg
     3901 cttgttttat gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta
     3961 caagttggtc agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat
     4021 gaataaagag gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg
     4081 cagtacatta ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc
     4141 ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt
     4201 agcggaagaa gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga
     4261 tcatttaatg accactttat tgctgagaga tctgattcaa gactttattg tactgtacag
     4321 acagaatcaa ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg
     4381 gaaaccattt tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt
     4441 tttaacagcg atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc
     4501 tgagattttt ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa
     4561 ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga
     4621 ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc
     4681 ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga
     4741 atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt
     4801 atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac
     4861 gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt
     4921 aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt
     4981 gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc
     5041 gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc
     5101 ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc
     5161 ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc
     5221 aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt
     5281 tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc
     5341 acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt
     5401 ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc
     5461 ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt
     5521 tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc
     5581 atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga
     5641 aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga
     5701 tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgcgatgtt
     5761 agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg
     5821 tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca
     5881 agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt
     5941 tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt
     6001 tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta
     6061 aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag
     6121 catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat
     6181 ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata
     6241 atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa
     6301 gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa
     6361 atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga
     6421 aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt
     6481 acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg
     6541 ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag
     6601 aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc
     6661 gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag
     6721 gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg
     6781 gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg
     6841 cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa
     6901 cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat
     6961 atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg
     7021 cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa
     7081 atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa
     7141 acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg
     7201 cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg
     7261 gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta
     7321 cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc
     7381 aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat
     7441 tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt
     7501 atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa
     7561 tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag
     7621 gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt
     7681 tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa
     7741 cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac
     7801 gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc
     7861 gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa
     7921 agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag
     7981 ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta
     8041 acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt
     8101 ttagctattg aagaatgttg atttataggg attccccctc catattatgt tggctcttta
     8161 ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg
     8221 ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc
     8281 aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta
     8341 agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg
     8401 ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc
     8461 gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg
     8521 accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc
     8581 acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc
     8641 tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc
     8701 gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag
     8761 ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg
     8821 ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat tgactttgat
     8881 gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg
     8941 tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag
     9001 aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc
     9061 aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc
     9121 ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg
     9181 acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc
     9241 aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa
     9301 gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg
     9361 atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga
     9421 accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc
     9481 taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc
     9541 gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg
     9601 gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata
     9661 ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt
     9721 caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat
     9781 gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta
     9841 tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt
     9901 tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat
     9961 aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc
    10021 atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga
    10081 cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat
    10141 caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa
    10201 caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct
    10261 ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac
    10321 gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac
    10381 gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg
    10441 cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca
    10501 agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga
    10561 tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat
    10621 cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac
    10681 ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat
    10741 tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata
    10801 atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg
    10861 atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc
    10921 tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac
    10981 ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg
    11041 tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt
    11101 gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga
    11161 aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca
    11221 aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta
    11281 tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt
    11341 gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa
    11401 acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt
    11461 cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata
    11521 gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt
    11581 tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca
    11641 gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa
    11701 gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt
    11761 cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc
    11821 tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt
    11881 tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga
    11941 atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga
    12001 ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg
    12061 tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc
    12121 aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc
    12181 tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa
    12241 tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa
    12301 aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga
    12361 tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc
    12421 gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt
    12481 tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg
    12541 ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg
    12601 gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat
    12661 acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg
    12721 cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg
    12781 aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta
    12841 tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca
    12901 gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga
    12961 ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc
    13021 gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca
    13081 agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa
    13141 cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat
    13201 aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc
    13261 cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac
    13321 aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga
    13381 agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt
    13441 ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct
    13501 tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt
    13561 tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct
    13621 ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc
    13681 agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat
    13741 cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag
    13801 ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca
    13861 agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg
    13921 gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga
    13981 gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc
    14041 tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg
    14101 attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat
    14161 ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat
    14221 tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa
    14281 agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga
    14341 ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc
    14401 aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga
    14461 acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa
    14521 cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt
    14581 cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga
    14641 tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac
    14701 atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc
    14761 tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg
    14821 tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa
    14881 cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg
    14941 agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc
    15001 atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat
    15061 gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat
    15121 caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct
    15181 tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac
    15241 acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc
    15301 cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt
    15361 gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct
    15421 gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat
    15481 tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca
    15541 acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga
    15601 tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag
    15661 aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat
    15721 cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa
    15781 cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata
    15841 caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg
    15901 caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg
    15961 aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg
    16021 ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat
    16081 tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta
    16141 tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat
    16201 tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg
    16261 accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat
    16321 tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg
    16381 acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc
    16441 taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa
    16501 tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata
    16561 gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt
    16621 atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt
    16681 caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg
    16741 ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc
    16801 aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa
    16861 attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc
    16921 ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca
    16981 agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt
    17041 gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg
    17101 cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca
    17161 gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt
    17221 ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg
    17281 acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag
    17341 tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca
    17401 gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa
    17461 ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag
    17521 cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa
    17581 gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc
    17641 gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc
    17701 ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa
    17761 catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca
    17821 ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg
    17881 ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga
    17941 acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt
    18001 atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga
    18061 ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac
    18121 aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt
    18181 gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat
    18241 gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag
    18301 ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact
    18361 ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc
    18421 catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga
    18481 aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc
    18541 agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt
    18601 ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat
    18661 cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt
    18721 tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac
    18781 atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat
    18841 gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg
    18901 cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa
    18961 gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta
    19021 tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc
    19081 agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac
    19141 ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat
    19201 cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca
    19261 gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt
    19321 tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca
    19381 agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga
    19441 actataatgt cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca
    19501 acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac
    19561 gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg
    19621 atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta
    19681 ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt
    19741 atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca
    19801 tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca
    19861 ggattagcag atattccggt cattggacca attttcttta aaaatacatt cttacctgct
    19921 tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc
    19981 ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta
    20041 tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct
    20101 gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc
    20161 atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca
    20221 ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct
    20281 tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt
    20341 gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa
    20401 taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt
    20461 gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc
    20521 tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg
    20581 ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag
    20641 agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc
    20701 aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact
    20761 gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg
    20821 atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt
    20881 atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa
    20941 ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt
    21001 tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata
    21061 tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa
    21121 gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag
    21181 aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa
    21241 tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta
    21301 tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg
    21361 gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc
    21421 cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa
    21481 acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca
    21541 gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa
    21601 cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat
    21661 taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga
    21721 aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg
    21781 ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga
    21841 gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc
    21901 tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc
    21961 catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat
    22021 tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat
    22081 taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat
    22141 gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa
    22201 agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg
    22261 accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt
    22321 agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg
    22381 aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt
    22441 tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac
    22501 attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga
    22561 tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt
    22621 ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat
    22681 ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct
    22741 atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg
    22801 gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca
    22861 gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca
    22921 atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa
    22981 atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg
    23041 aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat
    23101 accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa
    23161 caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag
    23221 cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca
    23281 agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt
    23341 ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc acaaggaaca
    23401 atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg agaagttggc
    23461 aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt
    23521 tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt taattcataa
    23581 acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga gataccttcg
    23641 atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac agttttgttg
    23701 tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc taatgtcact
    23761 ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt
    23821 gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta agaacgacgc
    23881 cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt caaaccttgt
    23941 aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac ccaaagttga
    24001 tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac agaagtgtaa
    24061 gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt tgcttcttcg
    24121 attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa tgcattccat
    24181 tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc ttttaagata
    24241 ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg aagagatttc
    24301 catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa aaaacgaaca
    24361 gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat ttctttttca
    24421 ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga agaaataaat
    24481 tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt ttttgcatcg
    24541 atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc aactaatgta
    24601 cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg gaaatagatt
    24661 tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag cccgcgatct
    24721 ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg
    24781 caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa atgaacagca
    24841 gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca accaaagtca
    24901 gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg tgcataacca
    24961 ataattttca tggtgattcc tttctagcag tgattttcta actggatata acagtaaaaa
    25021 tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg tcttttttta
    25081 ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat ctcaacgcct
    25141 ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg ttctactacc
    25201 ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac ttctagatgc
    25261 agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc gtctgtacgc
    25321 aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga gccataaggg
    25381 atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga agaatcaacg
    25441 atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa agcagcgccg
    25501 ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat tgtcaaatcg
    25561 actacgggga cttcatcaat actctttaac ggtattccta atttttccgc ttgttctttt
    25621 gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat acggcgacca
    25681 atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat catcccgtcc
    25741 tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt tgcgtccacc
    25801 tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac ggtaaaaaga
    25861 taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat
    25921 gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct gtgaagaaac
    25981 gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt tattttttat
    26041 cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga aggaggaaac
    26101 aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag gtggaaaaat
    26161 ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa actaacgtaa
    26221 actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct aaaaaaccga
    26281 actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt
    26341 atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta cgcggtggtc
    26401 gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta gatactgctg
    26461 gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct aaagcttaat
    26521 tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac tatgaacaat
    26581 atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt
    26641 accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg taatggttga
    26701 cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga tcaaagaatc
    26761 tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc aacctgttct
    26821 tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg aagttcgccc
    26881 tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga
    26941 acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca acacaggtgc
    27001 ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg cgtttgctca
    27061 ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct cagatagtgt
    27121 ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga ccagagaatt
    27181 ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg gtaaaacgac
    27241 tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg aaactcatga
    27301 aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta cgatcacttc
    27361 tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca caccaggaca
    27421 cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt
    27481 tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaacaac
    27541 ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg ctgacttctt
    27601 atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa tccaattacc
    27661 aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga aggctgaaat
    27721 gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag aataccgcga
    27781 attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg atgaagaact
    27841 tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag aaggtatccg
    27901 ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat ttaagaacaa
    27961 aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt tagatatccc
    28021 agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg cagacgacag
    28081 cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag gtcgtttaac
    28141 tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa acgcaacaaa
    28201 aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc gttcagaaat
    28261 ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca caactactgg
    28321 ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat tcccagaacc
    28381 agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa tgggtgtggc
    28441 tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg tggaaacagg
    28501 cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg accgtatgcg
    28561 tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt
    28621 ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta aaggtcaata
    28681 cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa
    28741 tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa ctggtttgaa
    28801 agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca aagctaaact
    28861 ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg ttgctgcttc
    28921 tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac caatcatggc
    28981 tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg taactgctcg
    29041 tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg ttcgtgcgat
    29101 ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta ctcaaggtcg
    29161 tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag tacaagaaga
    29221 aatcattaag aaaaacggcg gaaaagctga ataatttctt gaaaccggat agtcctgcaa
    29281 agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa ttatcgatga
    29341 tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata aaaaatatat
    29401 tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca
    29461 cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa cagctgcaat
    29521 cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg
    29581 tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg agtatgaaac
    29641 agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg ttaaaaacat
    29701 gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg ctgacggccc
    29761 aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc cttacatcgt
    29821 tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat tagttgaaat
    29881 ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc ctgtagttgc
    29941 tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt
    30001 aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca aaccattcat
    30061 gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta caggtcgtgt
    30121 tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg ctgaagaaac
    30181 ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact acgctgaagc
    30241 tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc aacgtggaca
    30301 agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag aagtatacgt
    30361 gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc gtccacaatt
    30421 ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt
    30481 tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg ctatcgaaaa
    30541 cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga
    30601 aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg acgtaatcgc
    30661 tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca ggatcaggaa
    30721 aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa
    30781 tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata
    30841 tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga
    30901 tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg
    30961 tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga
    31021 tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat
    31081 cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc
    31141 cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt
    31201 cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac
    31261 caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca
    31321 aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga
    31381 aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg
    31441 tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag
    31501 ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc
    31561 atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc
    31621 taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta
    31681 ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac
    31741 aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc
    31801 cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc
    31861 gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga
    31921 tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag
    31981 ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac
    32041 aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta
    32101 atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac
    32161 acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag
    32221 gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag
    32281 tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag
    32341 caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct
    32401 tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg
    32461 actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta
    32521 acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa
    32581 atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat
    32641 gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga
    32701 tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc
    32761 ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa
    32821 attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac
    32881 attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga
    32941 taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat
    33001 ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa
    33061 tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta
    33121 cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa
    33181 gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc
    33241 gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa
    33301 gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca
    33361 ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt
    33421 ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac
    33481 gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca
    33541 atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct
    33601 cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca
    33661 cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt
    33721 caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt
    33781 cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc
    33841 accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt
    33901 gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca
    33961 gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca
    34021 gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact
    34081 tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat
    34141 ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc
    34201 tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc
    34261 aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc
    34321 agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc
    34381 ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc
    34441 tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat
    34501 aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg
    34561 atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc
    34621 gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg
    34681 caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag
    34741 gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc
    34801 atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa
    34861 tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac
    34921 gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag
    34981 cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg
    35041 cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag
    35101 tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat
    35161 aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg
    35221 ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc
    35281 acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa
    35341 acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat
    35401 tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg
    35461 tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg
    35521 tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg
    35581 tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag
    35641 aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag
    35701 aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc
    35761 gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc
    35821 aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg
    35881 ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat
    35941 acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa
    36001 ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta
    36061 ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac
    36121 acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa
    36181 ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga
    36241 tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta
    36301 aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat
    36361 ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct
    36421 ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac
    36481 cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta
    36541 aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac
    36601 tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac
    36661 accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc
    36721 atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag
    36781 aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa
    36841 agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag
    36901 taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg
    36961 aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata
    37021 aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa
    37081 aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc
    37141 gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac
    37201 gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta
    37261 ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta
    37321 tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac
    37381 aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa
    37441 tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac
    37501 gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg
    37561 aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt
    37621 cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac
    37681 tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat
    37741 taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat
    37801 cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg
    37861 tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga
    37921 cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt
    37981 tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg
    38041 gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg
    38101 ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac
    38161 caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg
    38221 aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc
    38281 gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac
    38341 taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt
    38401 actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca
    38461 cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc
    38521 gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg
    38581 tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag
    38641 cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc
    38701 cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa
    38761 gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt
    38821 cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct
    38881 aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc
    38941 tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt
    39001 aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt
    39061 ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta
    39121 gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat
    39181 tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc
    39241 tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtagga tttggtacag
    39301 gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact
    39361 tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg
    39421 gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag
    39481 ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa
    39541 atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag
    39601 aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac
    39661 aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca
    39721 acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc
    39781 taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat
    39841 ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg
    39901 aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat
    39961 ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg
    40021 gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag
    40081 gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct
    40141 ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg
    40201 aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag
    40261 cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg
    40321 tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat
    40381 cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg
    40441 cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt
    40501 ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat
    40561 tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg
    40621 gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt
    40681 agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt
    40741 aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc
    40801 cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg
    40861 cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat
    40921 ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt
    40981 ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc
    41041 tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta
    41101 tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat
    41161 tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta
    41221 caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat
    41281 tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc
    41341 ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga
    41401 agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt
    41461 agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt
    41521 aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga
    41581 aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt
    41641 tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa
    41701 tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta
    41761 ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg
    41821 gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg
    41881 gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct
    41941 ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa
    42001 aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg
    42061 gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag
    42121 ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac
    42181 ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt
    42241 attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat
    42301 ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta
    42361 tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga
    42421 gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt
    42481 tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg
    42541 attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta
    42601 gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt
    42661 atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt
    42721 attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag
    42781 taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg
    42841 ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc
    42901 tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc
    42961 acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg
    43021 tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc
    43081 agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa
    43141 acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg
    43201 tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg
    43261 caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa
    43321 tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt
    43381 caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc
    43441 tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc
    43501 agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa
    43561 aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt
    43621 gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg
    43681 tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac
    43741 ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa
    43801 aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg
    43861 ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca
    43921 tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag
    43981 aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag
    44041 aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta
    44101 tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag
    44161 gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg
    44221 aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg
    44281 ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata
    44341 aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt
    44401 ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa
    44461 atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga
    44521 tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata
    44581 ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac
    44641 gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag
    44701 aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca
    44761 catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac
    44821 gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca
    44881 ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg
    44941 aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac
    45001 tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga
    45061 agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc
    45121 acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt
    45181 tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca
    45241 tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata
    45301 aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag
    45361 gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat
    45421 tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat
    45481 aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa
    45541 acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg
    45601 gatgaagaaa atatttggac gatccgacag atggttggaa tggtttttca aaatccagac
    45661 aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg
    45721 attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct
    45781 gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt
    45841 gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta
    45901 gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa
    45961 ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta
    46021 gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt
    46081 cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg
    46141 aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg
    46201 ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac
    46261 aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag
    46321 ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa
    46381 caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt
    46441 taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg
    46501 aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag
    46561 aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg
    46621 aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc
    46681 ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg
    46741 gcagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg
    46801 tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg
    46861 aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc
    46921 taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta
    46981 aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc
    47041 attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga
    47101 gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc
    47161 ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta
    47221 ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg
    47281 atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact
    47341 tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt
    47401 ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct
    47461 ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca
    47521 gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac
    47581 gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac
    47641 ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc
    47701 aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga
    47761 agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt
    47821 gtattattaa ctgtgctact gatcttttta agaggataaa cgttcagtgg gccgctatct
    47881 attcttttct tgctgatctg tttgcaagaa aggatgcaag cagtgtatga caagaaatat
    47941 tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga
    48001 aaaaatttta tagaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa
    48061 catggtacgt tacaaagcaa tcattgcgta cgacggaaca aattttaatg gattccaaaa
    48121 acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa
    48181 tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac atgcgatcgg
    48241 gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct
    48301 agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt
    48361 ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc
    48421 acgcagtcct tttcgacgcc attatgcaag ttattttcct tacccacttg atttggaaaa
    48481 aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc
    48541 aggaagctcg atagaggata aaatacgtac gatctacgag gctaagatgg aggtaaatga
    48601 aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg
    48661 gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc
    48721 agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacacc ctgaaggact
    48781 ttatttgtat gaagtgaaat atgaataaga ggttatgata gaagtggtca gcttcaagaa
    48841 ataagaaggt atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg
    48901 aagaagctac ttctggaaca ccgtttaagg aagaggtcgt gaaatcagcg ttttgacctg
    48961 agtattagag aaacaaacgg gaaaatagct cctcatattt tgccgtttgt tgagctaata
    49021 ccgaaggtca gctgattgaa caccgtttaa gaaagaggtc gtgagcctgt catttaactc
    49081 tgagtcacaa agagcaattt caaaaaacag cttctcatct ttgatgaata tcgtgacttg
    49141 tggacgaggc gttgataagt gaatcatctt tttgttacct attttttctg taaccaaaaa
    49201 acgatggaac gttgttttga ttgcccttgg tgcggacagt ctgaacaggt tccatcgttt
    49261 ctttattttc tacgtcttct tctgtgtgtg tttcgtggtt gttcttctat tgctggatca
    49321 atcgtgccat aaaaggggcg attgcggtca aggtaccaga cataaagaat aaacaatgca
    49381 atacatagga caaaaaggat tgcacctatc gtaaatcctt caggcaagta actgaaagtg
    49441 acagtatgtt tacctgctgg aacttttatc atcaagaatg cgtctttgaa attgctgacc
    49501 ttgactggtt tcccatcaat tttagctttc catcctttgt cataaggaat agttgtcacc
    49561 aacatggaat cttcagcggt atttacagtc ccagaagctt ttctgccagt ggcttccatt
    49621 tcgacacctt tttctttgat ctgattcatt gcggcttgat aggctacaac atctagacca
    49681 acgactttag gctccatgaa acttatttct ttcgtcccat aaaaacttgc agtaaatgtc
    49741 actgttgtct cttgaggata atagccaaga tcatagtatt gaccagaaat gttgatttgc
    49801 gtttttcgcg atgtaccatt gacagtgatc gtcgcactag aactttctaa ttgcgcaaaa
    49861 ttggttggga acaaacttaa gtaagcttgt gtatttgcag gcacatggac ttcccaagtg
    49921 agctcttttg gctggttatc tgctgtttct gagtaagtga cgccagtcgt tgtctttgtg
    49981 atcgtcgtat tcaactgatt cttcaaagta ggtgtataga atgtaaaata ctgttcattt
    50041 agaccagaca atgtattgat caagtttgtc tgactggtca aattatctgt cactggctgc
    50101 tcaacatcat taacggacaa cggtgcagta aatgctagtg gcaatgcatt gacattctga
    50161 tacagtgtat agtcgccgct ttgaccagct tggcggaagc catatttact gaacgtgctt
    50221 tcgtttttag aaatgttata tttgattcct gtaaacccat ccatcagcaa agtattattg
    50281 ttataacgga tattcaaatt tgtccctctt gaacggaaac ctaatttatc caggtaagag
    50341 gaagaattac gatttcttat agaagaaaac aaactgatac cactgtaacc atagttgatc
    50401 gaatcgtttg cagatactgg atccagattt tccaagcggt aaaacgagtc ggaatctttt
    50461 tttgtttcat ttacaagagc tttcaagtca ggataaggat cagtatataa actgcgggaa
    50521 gcataattcc aatcatcaag aatgccatgg atcatactgt tcgtattgac cagtgcttca
    50581 gcagacatga tcaataataa caatatactc agatgattga atgaaagttt tttatgctga
    50641 taaaatccca cagcaaaagc ataaatccct aagaaaacca ctgtcaatac aaaggaagtc
    50701 atcgtcacat aggtgtagct tccttcaggt ttgatgcccc atgccaccgc aaagagagca
    50761 gctacgacca aaatgattcc taacaacagc ccacgatcgt cttgttctat cttttcccat
    50821 ccttttcctg caagcaggag cactaagaaa gagaacagat aggcataacg gaataaaaac
    50881 atattcggtg catgcatacc atgccagaac aaattcaatg gaacaagata aaaacttgcg
    50941 ataagtatgg cgaaaagact gccgtacaaa aatttatttt tcaacggtac tttccgactg
    51001 gcaaaataaa acagacagag gactaatggt agcagacctg cgtaaacgaa cggaatcgat
    51061 ccgtattttg tcgtgtcata aacaccaatc atatttttca tgattagatc cagataagaa
    51121 gtcgcttctg ttttcaacgt agtgatctga ctcagtgcct cgccatttga ccgaagatcc
    51181 agcactgctg gcaatacaat gatcattgaa gcgccgcctg ccagtaaaga agtgatacca
    51241 taaggaacaa tcgttttttt atattctgac cagtttgtca atagacgggc gataaaatat
    51301 aagaaagaaa atacgccaat cataaaaccc atataaaaac tggataaaaa caagagtaaa
    51361 taactgataa ataaaagttt cggccgttta agatccataa cacgatgaat ccccaaaatc
    51421 actaatggaa gataaacaaa tgcatccagc cacatgatga tctccgaatg tgcagtgata
    51481 aatgagatca atgcgtaact gacgctaagt gcaacatgcc cccagcgagg aatcttgaac
    51541 gtacgtgcaa gaaaccaaaa agccaatccg gcacagccga ccttaagcaa agtcagaaca
    51601 tacagtgcat ctggcatcat ctggtttgga aaaaagaaca ccagtggcgt gaagatcccg
    51661 cctagatagt aagaaattaa agataaataa ttcaatccta gagaagcatt ccatgtataa
    51721 aaaatacttt gctttccaag aagaacattt cggaagctgg catgaaaatt agaaaactgt
    51781 gaaaaggcat cacttgccaa aatacttctg ctgcttccag gatagattcc gatacttaga
    51841 taaacaagtg ctaaaatcaa aaacggaatc aggaaactag ctgctgcgta gcgtccgttt
    51901 tctctaaaga atgactttaa attttttttc atatttattt tccaccaaat ttctatgaag
    51961 ttgtattcag tttatcatat ccttgttttt ctctgcttaa aaagagtcta gttttcacaa
    52021 aaaaataaaa tttagaataa ataacttcat aatttgttcg tataacaaaa ataaacgcaa
    52081 aaaaagggta ggaatcaaaa gtcccaccct taaaaatttt tattaaataa aaaaccgttc
    52141 actatctgaa cggtccaagt atcattcaat taaacgcgtt caacttttcc tgatttcaaa
    52201 gcacgagttg acacccaaac ttttttaggt tttccgtcga ccataacgcg aactttttgt
    52261 aagttaggtt taacagtacg ttttgttgag ttcatagcgt gtgaacggtt gtttccactt
    52321 tttgtcttgc gacctgtaaa gtaacaaact tttgccatgg taatttcctc ctttgccttg
    52381 atttggagca atagaataat tcagctcata ccagactaac atatcatata gaatgggaat
    52441 ttgcaagtac attttcatag atacaattga caattgagaa gaataattgt aaattaaaca
    52501 aggttatacc tgaaattttg caaggttttt gagtactttg ggtaactgcc ttttatttca
    52561 agttgcgcta tgataaaata tgatagaaga aaaatgggag ttttttaccc gttaaggagg
    52621 ctcattcatg gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta ccaatgatgt
    52681 aattgcaaca gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag gaatggccag
    52741 caaaaatcaa attaaagata atattactga gattttgcgt aaagaaaatt actcaagagg
    52801 agtagttgtc cgccaagaag ataatggtat tgctgtagat gtttatacga ttatcagcta
    52861 tggtacgaaa atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat acaatctaga
    52921 ggcattatta ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg ttcgtgtact
    52981 gccagactag gcaggagact ggctggtaac tcagcagtcg atctgaagga ggattatcta
    53041 ggtgagtgta acagaaataa gtgcaagcca gttccaggaa atggtccagg caggtgcgaa
    53101 tcgtctgcaa gtaaatgcag aatacgtcaa ttcattaaat gtattccctg tgccagatgg
    53161 cgatacggga acaaatatga atctatctat gactagtgga gctaaggcag tagtagattc
    53221 aacctcagaa aaagtaggag aattagcagc aatgctttca aaaggattgc tgatgggtgc
    53281 gcgcggaaat tccggcgtta ttttgtctca attgtttcgt ggattttcta agcaaattcc
    53341 agatgttgtc tcattaaatg cttctgatct tgctgcagcc ttcactcatg gagttgaaac
    53401 tgcgtataaa gcagtaatga aaccagtaga aggaacgatt ttgacagtag ctagagaagc
    53461 agcgaaagct ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag taatgacagc
    53521 tgttgtaaaa ggagcaaagc gtgctttagc taaaactcca gacttgcttc cagtcctaaa
    53581 agaagtcggt gtcgtagaca gtggagggca aggactgctg tttgtttatg agggcttttt
    53641 aagtgcattg aatggagaat tccaagcgga tgaaacatat gaaccttctc cagcagaaat
    53701 ggatgatatg gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg cgacagaaga
    53761 tatccatttt ggttactgta cagaaatcat ggtaaaaatc ggagaaggac ctacagtcga
    53821 tagtacattc gattacgaag aattccgtaa ctatttagat ggtataggag attctttact
    53881 tgtggtcaat gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc caggagaagt
    53941 cttgaattat ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca acatgcgctt
    54001 gcagcatgag acgattttgg aacacgatga agaagtgaac gagtttgcga aaaaagaaat
    54061 accacgtatc ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc aagagctgtt
    54121 tgaaagttta ggcgctaatt atgttatcag tggtggacaa acaatgaatc caagtacgga
    54181 agacattgtt aaagcgattc aagaagtaaa cgcagataaa gtgattgtct tgccaaacaa
    54241 taagaacatc ttcatggcag ctgatcaagc agcagaagta gcagatatcc cagtagcagt
    54301 tgttccttca agaacgattt cccaaggatt gacagctatg ctttcattca atgatcaagc
    54361 ttctttagaa gaaaacaaag aaacgatgac agaaatgttg tctagcgttg tgagcggtca
    54421 agtcactcac gcgatcagag atacctcaat tgacggtgta gagatcaaag aaggagactt
    54481 tttgggcatg atcgatggaa aaatcctgat ctctaaccca gatatcacag caacagcgtt
    54541 aagtacacta gaacaaatga tcaatgagga cacagagatc gtaacgatct tggcaggtga
    54601 tgaaggaaca gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa aacatcctga
    54661 tttggaaatc gagatccacc aaggagatca gcctgtctat ccttatctat tttctgctga
    54721 ataatttctg tgtcagaata tctttctaac acagtattca ttcataaaag gccttcggtt
    54781 agtgatcagg tatgatcgct gaccaagtcc tttttttgtt aaaggaggga ggaaaaattt
    54841 atgcgttctt tagatgatcc aatcactatg ctggcaggag tcggagaaaa aagagcagct
    54901 agtcttgcca gcttgggcat ccaaacgatt gaagacttac tgacttatta tccttttcgc
    54961 tacgaagata ttcaggaaag aaatctaaac gagatccagg atcaagaaaa ggttactttg
    55021 aaaggaatcg tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa aagccgattg
    55081 caatttcgta tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa tcaaccttat
    55141 ctaaaagaaa aagtcatttt atcggaggaa atcgccgtat acggtaaatg ggatgcaaag
    55201 cgtaaagcct tgaacgggat gaagatttta ggttctcaaa caatggatga cttttcgccg
    55261 atatatcacg tcaacaaatc aattcgtcaa acgacattag ttgatctaat caaaaaaggg
    55321 tttcaagaat acggcacatt gattgaagaa aatctgccaa acaaccttgt ggagaaatat
    55381 cgactgcttg accgagcttc tgctgtaaga agtatgcatt ttccaaaaga ccacgaagaa
    55441 aaccatcaag ccaaacgacg tgtcgttttt gaagagttct ttttatttca aatgcgtctt
    55501 caaggattga aaaaagcaga aaaagcagaa acgaatggtt tggagatcct ttatgatgtc
    55561 caaaaattga aacaatttac tcaaaagcta ccttttgaac tgacaaatgc acaaaaacgt
    55621 gtaacgaatg aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg acttttgcag
    55681 ggagatgtag gtagcggaaa aacagtggtt gccgcgattg cgttatacgc taccgttaca
    55741 gcaggtttcc aaggtgcgtt gatggtgcct acagaaatct tggcacaaca acatatggaa
    55801 agcttgcaac agctgtttga tcccaatgaa gtaaccatcg cattgattac ggggtcaacg
    55861 aaagcaaaag aacgacgtga gctccttgaa aaactagagc aaggggaaat taacgtcgtg
    55921 atcggcacac atgcattgat ccaagatgga gtcgaatttc agaatttagg tctagttatt
    55981 acggatgagc agcaccgttt tggcgtgaac cagcggaaag tacttcgtga aaaaggctgg
    56041 cgaccagatg tcttatttat gacagcgact ccgattccta gaacattagc tattacagca
    56101 tatggtgaga tggatgtatc tatcattgat gaacttccag ctggacgtat cccgatcgaa
    56161 acacgctggg taagaacacc tcaattggac agtgtactgg attggacgaa aaaagaactg
    56221 gcaagaggac atcaaatgta tgtcatctgt ccgctgattg aagaatcaga agcattagat
    56281 gtaaaaaatg cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc aaaatatgaa
    56341 gttggcttgt tacatggtaa aatgaagaat caggaaaaag aagtaatcat ggaagccttc
    56401 aaagacaacc agatgcagat attagtttcg actactgtaa ttgaagtagg ggtaaacgtg
    56461 ccgaacgcga ccgtcatgct gatcatggat gcggatcgat ttggattggc acagctgcac
    56521 caacttcgtg gacgtgttgg acgcggaagt gatgcttcct actgtatctt agttgctaat
    56581 cctaaaaatg aactaggtgt ggaacggatg aaaatcatga cagagaccac caatggtttt
    56641 gtactaagcg aaaaagatct ggaattacgt ggtcctggtg aagtatttgg cttccgtcaa
    56701 tctggtctgc ctcaatttgc gattgctgac ttggtgacgg atggaaatgt tttggaggta
    56761 gccagagaag aggctgcagc cctttggcaa ataaaggatt ggcagatgct tcctgagtat
    56821 cagcctttag cgcaaagcct ccaaatagaa aatcaggata atcgttattt cgattagcag
    56881 actgtgctat actttatact gatgacggaa aaaggagtgg aaacaatgaa aattgccgta
    56941 gatgcaatgg gcggagatca cgcacctcaa gcaattgtag aaggcgtggc tttagcgcag
    57001 caagattttc cagaagtaga attcttactt tacggaaaag aagcagaaat caagaaatac
    57061 ttaacaaatg aaaaaaacat cacgattatc catacagacg aaaaaatcaa tagtgatgat
    57121 gagccagtaa aagcaattcg acgaaaaaag actgcatcaa tggtccttgc tgcacaagca
    57181 gtgaaaaacg gtgaagcaga tgctgtattt tcagcaggga atacaggcgc gctactagca
    57241 gcagggttgt tcatcgtagg acgtatcaaa aatatcgaac gccctggctt gatgtctacc
    57301 ttgccagtag tagggaaaga aggtgccggt ttcgatatgc tggatcttgg cgctaacgca
    57361 gaaaacaaac cagaacattt gttgcaatac ggtattttag gttcttttta tgcacgtaaa
    57421 gtccgaggta tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga agcaacaaaa
    57481 ggtagtgaag tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag cttgaatttt
    57541 attggaaacg tagaagctcg tgagctactc aatggtgtgg ctgatgtagt ggtcacagat
    57601 ggatttacag gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa tatgatgaat
    57661 ctattaaaat ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc tatgctttta
    57721 aaagacggct tacgttcttt gaaagcagaa atggattatt ccaaacatgg cggtgccgtt
    57781 ttgtttggct tgaaagcacc agtcattaaa actcatggag caacaggccc cgatgctgtt
    57841 cgctatacga tccgccaaat ccacacaatg cttgagacag atgtagttgg acaattagtc
    57901 gaacaatttg aaaaaagcga agaatagtat ttggatatct gaagcgatcc gttccattga
    57961 aaaaaaggtg gacattcctg aaaaatgcca gtaaaattag ccatggtgta ggtaattgac
    58021 aagtgtatga ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta acaaagtagc
    58081 gaaaattatt tcaaatcatt ttgagataga taccgataaa gtgacggatg aattgaacat
    58141 caaagatgat ttaaaagctg attcaattag tattatggaa ttcgttttag agcttgaaga
    58201 tgaattcggt acggaaattt cagatgaaga cgctgaacaa attgaaacag tcggtggcgc
    58261 agtagattat atcagcagcc atcttaaata atttcaaata aaagcaaaga aggataaaaa
    58321 gctgcatgca gctttttatc cttctttttt taatcttttt gacacgtaaa agtcattttt
    58381 ctgataattc tggcgataaa cagaaattat gttgcaaagt aaagcggttt tctatataat
    58441 tattaataat tcattacaca aagataagaa taacctttgt tataaaacga tttattctgt
    58501 gtaatgtcct tttttattaa gaaaaacttc catttaggaa gcagagaggg gtattcgatt
    58561 gtccgataac caattattag aagttcagaa cctacataca ggattccgac tcaaagatga
    58621 ctattacgat gcagtagatg acgtttcatt tactttagac aaaaacgaga ttttagcaat
    58681 cgttggggaa tctgggtgcg ggaaaagtac cttggctact acgattatgg gattacttga
    58741 tcctaataac acaaaaatta caggtgagat tttatataac gatctgaatt taatcgacct
    58801 gaacgaaacg ctctataata aaatccgcgg aaatgatatc ggaatgattt tccaagatcc
    58861 gttgtctgca ttaaatcctc tcatgagaat cgaagaccag atcaaagaag gactttccta
    58921 tcacacaaag atgaatgcag aacagcgtca agctcgtgca ctagaattgc tagaacaagt
    58981 agggattcct aatcctgcga gagttggacg ccaatatccc catgaactat caggcggtat
    59041 gagacaacgt gtcatcatcg caatcgcaat cgcctgcaaa cctccaatcg tcattgcaga
    59101 tgaaccgacg acagccctag atgtaaccat ccaagcacag attcttgatt tactgaaaga
    59161 ccttcaggaa gaaacaaaaa cagggatcat cttgatcact catgacttag gagtcgtagc
    59221 agaaatggct gatcgagtgg cagtgatgta tggcggacag ttcgttgaag tagcaggtgt
    59281 aaaagaactg tttgatcatc ctaagcatcc ttatacacgc tctttactga actctatacc
    59341 acaagaagga aatcatgatg cagaactcca tgtgatcgaa ggtgtcgttc cttctctgaa
    59401 aaacatgcct cgtaaaggtt gtcggtttgc accacgtatt ccatggatac cagcaagcga
    59461 tcatgaagaa gagcctatcc ttcatgaagt cgcaccgaac cacttggtac gttgctcctg
    59521 ctataagcat ttccacttta gagacgaaaa aggagaggtg tagatgacag aattaatcac
    59581 aatcaaagac ctgaaagttc attaccctat tcgcagtgga ttcttcaatc gagtaacgga
    59641 tcatgtatat gctgtagatg gtgtggattt tattatcgaa aaaggaaaga catatggttt
    59701 agtgggagag tctggctctg gaaaatctac tacgggaaaa gccgttgtgg gattagaaaa
    59761 ggtaacagcc ggacaaatca tgtatgaagg aaaagatgta acgaagcgaa gcaaccgcaa
    59821 aaaaatgggt tataacaagg atgtgcagat gatcttccaa gattcaatgt ccagtttgaa
    59881 tcctaaaaag cgagtgttgg atatcattgc tgaacccatc cgaaattttg aacggttaag
    59941 cgatcaagaa gaaaagaaaa aggtaaaagg actgctagat atcgttggga tgccggaaga
    60001 tgcgctatat aaatatcctc atgaattttc aggcggacaa cgtcaacgtc taggagtagc
    60061 ccgtgctgtt gctaccaatc caaaactgat cgtagccgat gaacctgtat ctgctctgga
    60121 tctatctgtt caagcgcaag tattgaattt catgaaacga atccagcaag aatacggcct
    60181 cagctattta ttcatttcac atgatttagg tgtagtaaaa cacatgtgcg acaatatcgc
    60241 aattatgtac aaaggtcgat ttgtagaaat cggtacaaga gaagacattt ataatgaccc
    60301 gcgacatatc tatacgaaac gtctgctttc agctattccg caaatcgatg tagaaaatag
    60361 ggaaagccac aaagaaaatc gacgaagagt agaacaagag tatatccaaa accaaaaaga
    60421 atactatgat gcgactggtc gagtctacga tttgcgtacg atcacaacta cacacaaagt
    60481 ggcgttgaaa gatggaggtg caagctaatg tggaaaacga ttctgcgacg tgttttattg
    60541 atgatcccgc aagttattat tttaagtgtt ctgatcttct tattagccca agcgatgccg
    60601 ggggatccat ttaccggttt gatcaatcca aatcaggatc cggcagtgat cgaacagatg
    60661 cgacaagcag ctggcttgaa tgacccatgg tatgtccagt acttccgttg gatcggaaat
    60721 gctttgcaag gcgattttgg tcaaagtttc ttatacaaac gctctgttgc tgacttgatc
    60781 ggcgaacgga tcatcaacac tttatatcta tcgatcttga cagttgtcct tacttatttg
    60841 attgctgtgc ctctggggat gcttgctggc cggtatcaaa attcaatctt ggataagatt
    60901 gtcgtaatct acaacttctt tagttttgct gttcctttat ttatcttcgg cttgatcatg
    60961 ctatttgttt ttggttatcg tttaggctgg ttcccaacaa gtggatcaca aacactaggt
    61021 ttgcaaggtg gatttatcac tcaatggata gaccgtttga aatatattct gttaccatca
    61081 attacccaag catttttggc gactgccgtc acaatccaat atctgcgaag tgaagtaatc
    61141 gattcgaaat cgctcgattt tgtcagaaca gcccgttcca aaggggtacc gacgaataaa
    61201 gtattcagtc gacatatttt ccgaaatgca gcgttgccga tggcttctca aatggggtat
    61261 gaaatcactt cgttgattgc tggttctgta gttatcgaaa agatttttgg ttatccggga
    61321 gtcggaaaac tatttattga ttccatcggc caacgggact ataccgtgat cacatcctta
    61381 gtattgattt taggaatcgc gacgctagtg gggacattac tttcagatat catcatgagt
    61441 atcgttgacc cgcgtattcg gattcaataa aaccaatcag aaaggagaat ggatcgatga
    61501 gtgaaaaaaa caatacagca gttcaagaaa gtattcctcc tatggggata cgaatgattg
    61561 ctcgagaatt taaaaaagat aaaattgcta ttttttcatt aggtttgcta gtggttttat
    61621 tattagtgat ctttatcggt gcgatgctga ccgatcaaga caaagtgatg tatgttagta
    61681 tcttcgacaa atacgctgca ccaggaacag tttcgacaca aggacaaaag ttcattctgg
    61741 gagcagatga aggcggacgt gatgtattag gacaattgat cataggtgct agaaactctg
    61801 ttacgatcgg atttgcgatc acgatcctta cttcgatcat cggggttgga ttagggatca
    61861 tctctggcta ctacggaggg atcattgaca acatcttgat gcggatcgtt gattttatca
    61921 tgattctacc gatcatgctg atcattatcg tcttcgtatc gattatttcc agatacaatg
    61981 tctggtcctt tatattcatc atgagtgcat tttattgggt agctaaagcc cgattattcc
    62041 gaagcaagac attatctgaa gcaagaagag attatgtcag tgcctcaaaa acaatgggaa
    62101 ccaatgattt taaaatcatg ttccgcgaaa tcatgccgaa tctaagttca ttaatcatta
    62161 ctaatttgac gatgaacttt gcggcaaaca tcggtatcga gacaactttg actttcttag
    62221 gtttcggctt gccggcaaat gtacctagtt taggtacatt gatcggttat gcaagtaacg
    62281 gagaagtctt agtgaatcgg caatggatct ggttacccgc atcaatcctt attttagttt
    62341 tgatgttgag tatcaattat gttggacaag cattcaagcg ttctgcagat gcgcgccaac
    62401 ggttaggcta gtatagaagc gaagggggaa ataggaatga agaaaacgat ttttggattt
    62461 gcagcgctga tgtctgctgt cgttcttgca gcatgcgggg gcggaggtac agatacctct
    62521 ggaaacaact ctggaggcaa cggcaataac aaagaggaat taacttttcc aattgcaaca
    62581 aaaaataatg atgatgcaat cgaagacggc gaattagaag ctgcggtcgc aacggacagt
    62641 cagtttaaag gattgttcca gtgggagttt taccaagatt catatgatgc agcttttatg
    62701 gctccttctc atgaaccgct atttgcaaac gatgacgatt tcagaatcac agacgacgga
    62761 gctgcaacgc ttgatctaga tgaagatacg aaaaaagcga caatcaaagt aaaagacaac
    62821 gtcaaatggt cagatggtga agatgtaacg gcggacgatg tgattttccc ttacgaaatc
    62881 attgggaata aagattacac tggtatccgt tatgacgaca ctttccgtaa catcgtaggt
    62941 atggaagaat atcatgatgg aagtgccgac acaatctccg gaatcaaaaa aatagacgac
    63001 aaaacagttg aaatcgaata taaagaagta aacccaagca tgcttcaatc gggtggtggt
    63061 atctggtcat atgcagcacc aaaacatacg ttagaaggtg tagcaatcaa agatatggaa
    63121 tcaagcgatc aagtacgtaa aaatccagtg acttttggcc cttattacat gagtaatatc
    63181 gttgcaggag aatcagtaga atacttgcca aacgagcact actggaatgg tacgcctaaa
    63241 ttgaaaaaga tcacgatgaa atctgtaccg atggcaagtg caactgaagc attgaactct
    63301 aaattatatg atatgatttt ccaaatgcca acagatactt acgataccta taaagaaatt
    63361 gctggctata caaacttagg ccgtgaacaa acttcttata cttatctagg attcaagtta
    63421 ggtaaatgga atgcagatac aaagagtgtg gaatacaatc cagattcaaa aatggctgat
    63481 aaatcattac gtcaagcaat gggctatgca ttagataata atgcagtagg tgaacgtttc
    63541 tatgcaggtt tgagaagcaa tgcgacttca ttgattcctc cagtattcca gtcataccat
    63601 gatgatgaga tgaaaggatt cacacaagat ctagacaaag cgaaaaaact attagatgat
    63661 gctggctaca aagatgtgga tgacgatggt ttaagagaag acaaagatgg aaataaacta
    63721 acaatcaact ttgcttcgat gtccggtggg gaaacagcgc aaccattggc agattactat
    63781 atccaacaat ggaaagaaat tggtttggat gtccaataca caacaggtcg tctgatcgaa
    63841 ttcaattctt tctatgataa attagaaaac gatgatcctg aaattgatgt ttaccaagct
    63901 gcttggaata caggaactga tccaaatcca agtggactat ggggaccaaa tgctgcgttc
    63961 aactacactc gttatgaatc agaagaaaat acaaaattga tcaacgatat cagttcaagc
    64021 aaagcatttg atgaagagta ccaaaaagaa gcatatcaaa aatggcaaca atatgcattt
    64081 gaagaagcat ttgcgattcc aacgttgttc cgtaatgaag tattgccagt caacgaccgt
    64141 gtcaaagact gggattggtc ttatgatgta ccaaacccat gggcaaatgt ttctgttaca
    64201 gcagattcac gttcataatc actaaaaatg accgaaaaag ggcgatgtcc tttttcggtt
    64261 ttttgatatt tttctgttta tttctctaat attcaggtca aggcgctgat ttcatgactt
    64321 ctccaataaa cggtgttcaa agtccagttt ctcggtatta agtcaaattt atgcaaaaat
    64381 atggaaagcc attttcacaa aattttttct tcatactcga aactaaacga atttttcaca
    64441 acttctcgtc aatcactagg attattaaag ctttacttac cttttgaccg aaccatttca
    64501 aatttgacaa aaaaccagta taataaggaa gtatcgaaaa aatatccata aaaggaatgt
    64561 ggaaaatgga caaccagctg acaaaagaac taaaagaaaa ctataacatc gtttttcacg
    64621 atctaaattt gcttgaacaa gcttttaccc attcatccta tgtgaatgag catcgcaatc
    64681 tgcaattatc ggataatgag cgacttgaat ttttaggaga tgccgtttta gaactgatgg
    64741 tttctcagta tttattccgt ttatatcctc atttgccaga aggaaaattg acaaaaacac
    64801 gagctgcgat cgttcgcgaa gacagcttgt cgaaatttgc caaagaatgt cactttgatc
    64861 aatatgtttt gttgggcaag ggagaagaga attcaggagg acgtacgcga ccagcattgc
    64921 tttgcgacct attcgaagct tttttaggcg cgttatatct tgaccaggga ttcgacacga
    64981 cccatgcatt catcgaaaaa gtgatttttc ctaaagtcaa agcaggtgct ttttcacatg
    65041 agatggatca taaaacaaaa ctacaagaag tcttgcaaaa atcaggtgat gtatcaatcg
    65101 aataccgatt gatcaatgaa gaaggtccag ctcatgaacg agtattttgg atcgaagttt
    65161 atgtagatga tcaattgatt ggcactggac aagggaaatc gaaaaaatta gcagaacaag
    65221 ctgcggcgga aaatgcattg gcagcactgt aaaagtaagt gagggaaagc tttgtatttg
    65281 aaacgaattg aaatagccgg ttttaaatca tttgcagaca agacagtcat tgattttgaa
    65341 aatagcgtca cagcagtggt tggaccaaat ggcagcggaa aaagcaatat tacagaggct
    65401 atccgctggg tattaggaga acaatctgct aaaagtctgc gaggcggaaa aatgccggat
    65461 atcatctttg ccggttccga tacccgtaag cagctgaata ttgcagaagt cacagtgatt
    65521 ttagacaata gtgatcatta tctgccattg gagtacaatg agatcagtgt aacccgccgt
    65581 tatcgacgga ctggggaaag cgagtttttc atcaataaac aaagctgcag attaaaagat
    65641 atccaagaat tgttcttaga ttctggatta ggaaaagaat cgttctcaat catttctcaa
    65701 ggaaaagtag aagcgatttt cagtagtaaa ccggaagacc gtcgtgggat ctttgaagaa
    65761 gcagctggtg tcttgaaata caagcagcgg aaaaagaaag cagaacagaa gctttttgaa
    65821 acagaagaca acctcagccg cgtccaagac atcatccacg aattggaaga acagttaact
    65881 cctttggccg cccagagtga ggcagcaaag gagtttttac ggctaaaaga aacactgact
    65941 caaaccgatg tctctttgat ggttgctgaa atcaagacag caaaaaaaga ttgggacaac
    66001 aaacaagcac agcttgcgaa attcaatcta gaattaggca aactatctga gtcgatccaa
    66061 gaacaagaat ccattttggc gaagcagcgt aaagaaaatg cgcaagccga tcgtctgatc
    66121 gaaaaaaatc agcaagtatt attagatctt tcggaaaaac tgaagcagac agaaggacaa
    66181 aaagatgttt tgcaggagcg gacaaagcat acacaaaaaa gctcgcaaga atatcaaact
    66241 tcattagcag aagcacaaaa aaaggttaaa cattttgaaa agcttcaaga aagcttgatg
    66301 aaagcagcag cagaaaaaga aacagagatc caaaaagctg aagcaaatct cataaaaaca
    66361 cagcaagaat tagaaaaata ccaaaaatca acgaaagaat tattggcaga attaagagat
    66421 caatatgttg atttgatgca agaacaagca gctgtaggaa acgaactgaa gtatctagaa
    66481 cgtcaatata tacaagaaac agcaaaaagc aagcagacgt tagccaagca atcggaagta
    66541 gaagcatcag tagaccgatt gatcttacaa aaagaagaat tgacacaaaa gcaggcgcag
    66601 ctaaaatcat ctttaactga aacaaaagag aagttagaaa tgatccaaca aaacgggaaa
    66661 aagttccaag aagcattagc aaaagaacag cctaaaatgt atcaattaat gaatcaagtc
    66721 cagcagctac gtgctcgtaa gaaaagcctt caagaaatcc aagaaaacta ttttggtttt
    66781 taccaagggg ttcgattggt cttgcagcat aaacagcagc tttcgggtat tgtcggagca
    66841 gtggctgaac tgatcgatgt tcctgcagat ttcacgttag ctatcgaaac agctttaggt
    66901 ggggcagccc agcatgtcat cgttgaaaat gaaaaagatg ctcggcaagc catcacgtat
    66961 ttgaaacagc aaagaggagg aagagcaaca ttccttccac tgacgacaat caaaccaaga
    67021 caattaccgg ctcatatatt gacacaagca gcagctgtag aagggtttat agggattgcc
    67081 agtgaacaag tcagttatcc cgatcaaatc caaactgtgg ttcaaaacct gctaggaacg
    67141 atccttttag ccaaagactt aactagtgcc aatgcaatcg cgcaaacgat ccgctatcaa
    67201 tatcgagtgg tttccttaga aggtgatgtg atgaatgccg gaggatccat gacaggtgga
    67261 gcgaataaac gaggaaacca aggcagtttg tttgtacaaa atcaagaatt gaaacagttg
    67321 actagtgaat ttgaagaagc agataaacaa ctccaagcac aagaaaaaaa agttcaggag
    67381 ctgcaacaag aaacagctcg tttagccgaa gaacaagaag ttttgcgtac acgaggcgaa
    67441 caattacgtt ttgaagaaca agaagcgaca aatcagctac aaaacatcat aaatgaactt
    67501 gaacgttttg aaaaagaaaa gcaaatctca acttttgaga cacgtgaact gcagcagttt
    67561 atcgaagatt acgaaaagca aacgaacgaa ttgaaagaca aacaaacaga tcttgaatcg
    67621 caacgacaaa aaatcgacga agaaatcaaa tcgttaagtc aagaaagcga ccagatggaa
    67681 gccagaagag cacaagtaca aagtcaaaaa gcacaagaac aagcagatct tgctgtcttg
    67741 aaagagcagt ttaatcatct gcagatccag ctccgcggtg cccgagtcca aaaagcagag
    67801 gcaaccgaac gtcaagaagc gatagaaaag cagttggcga cattgacagc agacttttct
    67861 gatcatgaag tcacagaaga aagtttggaa aaacaaatta acgaattatc tgctcagcga
    67921 gaaacattaa aagccgagtt ggcaaaagcc aaagagcagc gagatagaag gcaaaaggag
    67981 atcgataagc ttgaagcagt cttggcagag cgtaatcgtg agcaaaaagc acgactgtcg
    68041 gaacaatcca aattagaagt ccaaaaagac cgagctgaaa tgattttaga taatcatttg
    68101 acctatctgc agtcagaata tcagctgaca tttgagaaag cttcccaaga ctatcaagaa
    68161 acaaccgata tcgaagacag tagaacaaag gtttcatcct taaaagaaca gattgaaaaa
    68221 ctgggaccag taaacttaaa ttcaatcgaa caatacgaac aagtcagtga aagacataca
    68281 ttcttagcaa cacagcgaga tgatctgtta gcagcgaaaa accaattgtt tgaaacgatg
    68341 gatgagatgg atgatgaagt gcggacaaga ttcaaagaag tatttgaagc tatccgtcaa
    68401 gaattcaaaa ttgtctttcc aaatatgttt ggcggcggta aagctgaatt agtactgacg
    68461 gaaccaactg atttgctgaa aacaggtatt gagattgaag tacaacctcc tggcaaaaaa
    68521 ctgcaaagtc taagtttgct ttctggagga gaacgagcac ttacagccat cgcgttgctc
    68581 ttttccatta tccgtgtctg tcctgttccg ttttgtatct tagatgaggt agaagcagcg
    68641 ttagatgaag ccaacgtgaa acgttttggt cgttacttga gcgatttcca agatgacaca
    68701 caatttatcg tagtcacaca ccgcaaagga acgatggtcg cagcagatgt actgtacgga
    68761 gtgacgatgc aggaatccgg tgtatcaaaa atcgtttctg tacggatgga agatatcaat
    68821 gaagaggcga aaatcaacga aacgaaagtg taggaacgaa aatgatcaaa ttagtagcaa
    68881 tcgatttgga cggtacatta ttaaatgaac aaaagctgat ttcagatgag aataaacaag
    68941 cattagcaca agcaaaacag caaggggtca agatcgttct atgtacagga cgccccttag
    69001 ctgcgatggc gcattatctg caagaattag gactggtaga tgaaggtgac tacagcatca
    69061 ctttcaacgg cggtttggta caaaaaaatg atactggtga aatcatggaa aagaaagtga
    69121 tggaagtatc agatatccaa cgcttataca aactggctca gcaattggat ctgccactag
    69181 atgttttgtc tgacaatgtg gttttacagc tgcctagtgc cccaaggaag caatccttgt
    69241 acaatgtgtt gaataagctg ctgcaattcc aaccagcgga tataagcgat attacagatg
    69301 aatttgtgct taataaggca gtaatcgctt atgaccaaga agaattagat cctaaaatca
    69361 aagaaatacc agcagaattc catgaaatgt atgagatcat caaaacaaga agtatgcttt
    69421 tggaatttat gccaaaaggt gttacaaaag cttatggtat ttctttattg gcaaaagatc
    69481 ttggtttgaa acaagaagaa atcatggcga tcggtgatga ggaaaatgat ttaccgatga
    69541 tccaatatgc tggactaggc gtagcgatgg agaatgctgt gcctttcgtc aaagaagcag
    69601 cagatttcgt gacagccagc aatgaagaaa atggcgtagc caaagtgatc caaaaatttg
    69661 tattagagta gggggagtca aatgggattt tttgataaaa tcaagcgtgc ttttactggc
    69721 gaagagaaaa cagaagaagt tgaagtacag gagaagtacg acaagggttt agaaaagacg
    69781 cgtaaaacat ttggtgaacg gatgaatgag ttgtttgcca attttcgaac agtcgatgaa
    69841 gacttttttg aagaattaga agaaacactt atcggtgcag atgtaggttt tgaaacagcg
    69901 atacgaatca ccgaagcatt acgtcaagaa gtgaaactca gaaatgcgaa aaaatcggca
    69961 gaagtccaaa atgcgatcat cgagaaactt gtggatctct atgaagcgga aggaatcaat
    70021 gaagtaaatg aactaaatat ccaagaagac ggactaacag ttattttgtt tgtcggtgtt
    70081 aatggtgtag ggaaaacaac aagtattggt aaacttgccc atcagttcaa aaacgaaggt
    70141 aaaaaagtat tgatggcagc agcagatacc ttccgcgctg gagcaatcga ccagttagtt
    70201 gtctggggcg aacgcgctgg tgttgaagtt gttcgtggca atgcaggagg cgatccagca
    70261 gctgtcgtat tcgatgcttt ggaaagagca aagacagagc aggcagatgt tttactagtt
    70321 gatactgcag gccgactgca aaataaagtg aacttgatga acgaattaga aaaaataaaa
    70381 cgagtaatcc agcgggaatt accagaagca ccccaagaaa cacttcttgt attagatgca
    70441 accactggtc aaaacgcgat ggttcaagca aaacaattca aagaaacaac agatgtgacc
    70501 ggtcttgttt tgacaaaatt agatggtaca gcaaagggcg gtatcgtact tgctatccgt
    70561 aatgaattgc atttaccagt gaaattggtc ggtcttggtg aaggaatcga tgacttggag
    70621 ccgtttgatc caaatgattt tgtcgttgga ttgttcaaag gattactaaa agaagaataa
    70681 cccatgtcta aaaagcagga gctgtgacaa aagttgcagc tccttgtttg aatgattgtt
    70741 tttaacatag ctgtgttttt gatgccttca tttttatact tatttgtaag cggttctcaa
    70801 acaatttcgt tctttttctt agactttttg attataatta gattcatatg aattggaggt
    70861 cacttaatga tgaattttaa gcaattatta ttgcatgtca atgcgcgccc ttttatcgac
    70921 caagctcgat ttggtataga acgggaagga cagcgggttg atcttgcagg aaatctagca
    70981 aaaaccgatc atccagcaat ctttggcgat cgatcctatc acccctatat ccaaacagat
    71041 tttagcgaaa cacaaacaga gatgatcacc cctgttaccg attctattcc cgaattattt
    71101 cagtatctcg ctgctgttta tgatgtgact gctcgttcta taccgaaaga agagatgatc
    71161 tggccattaa gtatgccacc tgccttaccg gaaaaagacg aagagatcat tattgcaaaa
    71221 ttaaaaaatt tcgaagatgt cttgtatcga cgttatttag caaaagaata cgggaagcga
    71281 aaacagatgg taagcggtat tcatttcaat tttgaatttg gcgatgagtt gctaagaaca
    71341 ttgttcagcc atcaggaaga atttcaagat ttttccgaat tcaaaacaga actttatttg
    71401 aaaacagcga gaaactttat gcgctatcgc tggatgatca cttatttatt cggtgcttca
    71461 ccaatgagtg agaagaacta ctttttagat gaatcacatc cgcaagaacc tgttcgcagt
    71521 attcgcaaca gtgcgctggg ctatacgaat catccaaatg tgaaagtttc ctatgcttct
    71581 atgaaacagt atttagcaga tatcgagcga atgatcgaag aagggaaact ttcggaggag
    71641 aaagaatttt atacaccgct tcgtttccga ggcggaaaga aagtcgcaga tttagcaaca
    71701 acaggtgttc gttatattga attgcggaat atcgatttga atccttatgc aagattgggt
    71761 atcaatccag agcaagttcg gttcttacaa ttattcctga tgtacatgtt atggacagaa
    71821 gaaaaagaag actgtgacca atgggtagca gaaggtacga ctcgaaataa caaagtagca
    71881 ctagaacaac catcagatca aacagaattt catcaagaag gtagagaaat tcttgaagga
    71941 atgaagcaaa tgctggttga attggattgg ttggattctc tttatttagt agaggaagca
    72001 ttgactcaaa tggatcatcc tgaacaaacg ttagcagcga aactctatca agaagcacag
    72061 ctatcgagtc agcaagaagt tgctgtggca ttgggacatc aatattataa agaaagccat
    72121 gaacgtccct atcagttggc cggttttcgc gaaatggaac tttctactca gatatttatg
    72181 ttcgatgcca tccaaaaagg tgtccaagtc aaagtattag atgagtcgga tcaatttttg
    72241 cgtctgcaat tccaagacca tgtagaatat gtgaaaaatg caaacatgac aagtaaggac
    72301 agctatatcg tcccacttat catggaaaac aaaacagtaa cgaaaaaagt cttaaaagaa
    72361 gctgggttcc gggtaccagg aggcgcagaa ttttcatcca tggaagaagc ggtaaaagct
    72421 tatccgagat ttgccgagca ggcatttgtg atcaaaccta aatcaaccaa ttacgggtta
    72481 ggtatcacga ttttcaagga aggagctagt ctggaagatt atcaagcagg tttagccatt
    72541 gcttttcgtg aagacagttc ggttttagta gaagaattta tgccgggaac ggaatatcgt
    72601 ttctttgtga tcgacggaga agtccaagcc atcatgttgc gagtccctgc taatgttatt
    72661 ggtgacagta tccgaacagt gaaagaactt gttgaagaaa aaaatagtga ccctttgcgt
    72721 ggaacgaatc atcgtgcacc tttagaattg atccaattag gtgaattaga acaattgatg
    72781 ttgaaagaac aagggttgac gatcgaatca gttccccaag ccaatcaaat tgtttatcta
    72841 agggagaact caaatatcag tacaggtggt gattcgatcg atatgactga tgaattttct
    72901 gaagcttaca aaaaaatcgc cgtttctgcg gtagaagcac taggagctaa gattagtggt
    72961 atcgatttga ttataccaga taaagaaatc gatcctacaa ctgataaaaa agcatatggg
    73021 atcatcgaag caaatttcaa tccagccatg catatgcatg tttatccttt tgcaggaaaa
    73081 ggcagaagat tgacgatgaa tgtattgaaa ttgttatatc ctgaagtatt ttaaaagaaa
    73141 aagcttttga cagattgtcc tatttgatgt caatctgatc aaaagctttt tttgcatgta
    73201 aaaatttatg tgttttttcg ttttaaaata aacgatccca gaagcttttt ttcttttttg
    73261 gtttttcttt ttcagctggc gaagagtttt cttctgggta ttttttttcg atatcaatca
    73321 caggttcatt tactgcttct tttgctgtta agagcaatcc gaattcctca ggatttgcag
    73381 tgacgtaatc gttgactaca gtgaaacggc tattgttttt cgttgctagc tggatatatt
    73441 ggttttggac agaaaggggt agagccccat tcaagagtat ggaagcatct ggatgttttt
    73501 tgagttcttt taaaaagttt tctttgtttt ttggttggat catttcagca acagtcatac
    73561 taagatagca gcgttccctg aatgtcccta aatatttatg ctgttcttca gggttcacta
    73621 aaggtgtacc ataaagtccc ttatccaaat ggctttttac atcatcttgt gccataaaaa
    73681 ataccctcct tcttctcttt gtctttctta cgattatagc atattcccaa taatacctaa
    73741 aacatatgaa aaagcggttg cttttgttca acgattcact taaaataaga atgaaaatca
    73801 ttctcaatta gaaaggtgga ttggatgcgc tacggacgac tagtagcatt attagttatt
    73861 ttaagtagta tttcattgtt catcggagta tcgaatgtac aattgtcaga actgctccag
    73921 ttagatgggc aaagcttaca aatcttgcta gtgactcgtt tgccacgaac gatttgtttg
    73981 atccttgtgg gagcgaccag tagtatttgt gggttgatca tgcaacattt gacccaaaat
    74041 aaatttgttt caccaacgac tgcgggcacg atggatagtg cgcgtttagg tattttagtg
    74101 gctatgattt tcctacctgg tgcatccttg attgtccgat ccttgacagc attttgcttt
    74161 gcttttgcag gaacattgct ttttttatcc cttacgcgat tgttcctcca gaaaaatcaa
    74221 ttgatgcttc cgttgattgg agtcatgtta gggaatatca ttggttcagt tgctactttt
    74281 tttgcttatc aattccagtt ggtacaaaat atgacttcat ggcttcaagg gaattttgcg
    74341 actgtgatac gaggaaatta tgagttattg tatgctgttg ttccattgtt gctcttggtt
    74401 tggctatttg cttaccgatt tacgatcatt ggtctgggcg aatcgtttgc agtaaatctg
    74461 ggaatagatt accagaagat gcagttttta ggcattggct tagtatcatt ggctagtgca
    74521 gtcatgttga tcatggtagg aagcattcct tttatcggag tgatcattcc gaatcttgtc
    74581 tctagaatat atggagatca agtgcacaaa acaattggcg taactgcttt agcaggtagt
    74641 ttgttcttag tattttgcga tcttgtcgct cgcgtgttga tttttcctta tgaaatacca
    74701 gtcagtgtga tcgttggaat catcggtggg gctatcttcc tttatcttct gataagggga
    74761 cgacgatatg cttaaaaaaa agccatctat ttttattggt atgcttgttc ttgttttatt
    74821 tgttacagga atgtacttga cgatccatac atatggaaac tggcattttg catgtgcatt
    74881 gaggagtaag aaaatttttg catttatcct tgtctcgcta gctgtcagca gtgccacaat
    74941 cagctttcag acaattacgc ataaccagtt tttgactcct tcagtattgg gacttgagtc
    75001 gttatatgtc cttgtccaaa cggtcctttt cttctttatc ggaggagtga cgatgcttca
    75061 gcagacaggt acagtttttt tcttattgaa cgttgggctg atggtcgggc tgagtctatt
    75121 gttatttttc tttcttttga aaaaaatgag agcgaatctt tttttattac tgatgtcagg
    75181 gatgatttta ggtacgcttt ttggtagtgt cagtaccttt ttacaagtca tcatggatcc
    75241 gaatgaatat gatttgcttc aaggcagatt atttgcaagc tttggcaata tccagagtca
    75301 gtatcttttg cctgcttctt tgatcattat cggctgctgg tacctgttat ttcgatatcg
    75361 acatgtgcta gacgttttcc atctcggaaa tgaacaagca gtaaacctcg ggatagacac
    75421 agatcgtttt gaatttttga ttttatgttg tgtcagtctt ttgacaggga catcaacggc
    75481 tcttgtgggt ccagttactt ttttagggtt tattgtcgca aatatcagtt atcgtgtctt
    75541 ttctacttat cgtcatggtc agctatttat cggaagcttt ctggtagcta tgctgtttct
    75601 gacaggagga cagctcgcag tagagcagat tttcggctgg aatacaacag tgagtgtcgt
    75661 cgttgaattt gtcggaggaa tttattttgt cacaaaattg attcgcgaaa ggaagagtat
    75721 cctatgattg atttacaaaa ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat
    75781 gtacagttaa cgattcctga aaagaagttg actgctttta tcggtccaaa tggtgctgga
    75841 aagtcaacgg tgttgtcgat gatcagtcgt ttgataccaa aagatacagg tgaaatctat
    75901 ttagaccata acgaagtaaa ggcttggaaa tccaatgaat tagccaaaaa attatcgatc
    75961 ctacgacaat cgaatgaaat aaatgtccaa atcaccgtga aagaattagt agaatttgga
    76021 cgatttcctt atacgaaagg aaaattgacg acaaaagacg aggaaatcgt tcaggaatca
    76081 ctggaacacc ttgggttaga agaaatagcg gatcaatcga ttgacactct ttcaggcggt
    76141 caactccaac gtgcgtatat tgcaatggtg ctagcgcaag acacggatta tattttactg
    76201 gatgaacctt tgaacaatct agatatgaat tttgcggttc aaatcatgca gattttaaaa
    76261 aatttagtac aagaaatcgg gaaaacaatt gttattgttc ttcatgatat caactttgct
    76321 gcaagttatg cagacgaaat tgttgcaatg aaagatggac agttatttac gcaaggaacg
    76381 acccaagaaa tgataaaaaa agatgtattg gatgaattat acgaaatgga catacggatt
    76441 tgtgagattg aaggccgcag attctgtctt tatttctaaa actaacatta ggagagaaga
    76501 gatgaaaaaa ttagtagtag gtatcttgat tctagcatca tttggtttag cagcatgtgg
    76561 aaatagcaac aacaccagtc aggcagacac aaaaaaaagc agcacacaaa ctgaaactac
    76621 attgacgata acagatagta atggagacca aatcgaggtg ccaaataatc cagaaaaagt
    76681 cgtcgtattc gacaatggtt ctttagatac gatggatgct cttggtgttg gagataaggt
    76741 agttggagca gctacaagca gccttcctga gtatctttca tcctataaaa aagtagaatc
    76801 agcaggtgga ataaaagaac cagacttaga aaaaattaat cagcttcagc cagatctaat
    76861 catcatttct ggccgacaaa gagattttca aaaagatcta agtcaaattg ctccgaccat
    76921 tttcctttct ttagatgcga aaaatccatg ggaatctttc cagcaaaatg tcactgcttt
    76981 aggggagatc tttggtaagc aagaagaagc taaaactcaa ttagaggaat tatctagtgc
    77041 aatcgatcag acgaaaaaga aagcagaagc tactgacaaa aaagcactag tcacacttgt
    77101 gaatgaaggg caattatcag cctacggatc tggttctaga ttcggtttca tccatgattt
    77161 atttggcttt gaacaagcag atgatcaaat cgaagcatcc actcacggtc aaagtgtttc
    77221 ttatgaatat gtgctggaaa aaaatccgga tatcttattt gtagtagaca gaacaaaagc
    77281 tattggtgga gacgacagca aagatgatat ttctgccaat gaactggtag cacaaacgaa
    77341 tgctggtaaa aatcagcaga tcatctccct tgaaccagat gtctggtatt taagcggggg
    77401 cggtttagaa tcaatgaaac tgatgatcga agatgtgaat caagcattta aatagcataa
    77461 ataacagagg attaaactct catactatgt aacaataatg tttcttacat tcttaaatag
    77521 caagagccta tcctcaaaga agttatcaaa aaccatccgt aaaatcgtat tgatgatttt
    77581 acggatggtt tttatttatt tgagaaatct tttttcaacg tgaattcatt atctacatca
    77641 tacgggttga ttctagaatg gggagtggca aaaaatagtg tgatccattt tttcagcatc
    77701 aagatcgcct cctttgattt gaatcaaagg tacaaagcga aacttagata aaacttaaat
    77761 ttagatattt ttattttgta ccgatctctt ctttaaacga tgttccatct gctgcttctc
    77821 ggcaataagt ccactagact agaaaatatg agaagctatt ttctgtccag cgttcttatt
    77881 actcaaagca aatcgcagtt gtcacaatct cttctaaaca atgttcaatc agctgacctt
    77941 cggtattagc tcaaagcact gatttcacaa cctctattta ggcatgtgtc tcagcaagcg
    78001 gttgatagtt gatgatctgc atttttcgat aaaaatcggc gggaagctta ctccaccgtt
    78061 ttaataattg atagaaataa ctctcttctg ctttgatttt atactcgctt aatatcaatc
    78121 caaaactatt cgtttttttt gaagctaatt ttttataaat atccaatgct ttggttttat
    78181 cttcattgac aaagagtgtt tttacttcaa tataagaacc ctcgatattt tgtgtttctt
    78241 cgataaaata gatgtttttt atatattcct tcatagtgat tcctccaaag aagtctttta
    78301 tttgataagt ttactctacc gaagagttgt taataatccg tgaacaaaaa gtaattcttt
    78361 ttccaatagt tgttaaaggt tttttatttt ttatagtatt ttttgttatt taagaaagga
    78421 ataaggttaa acaaataaca ggttgctttt tgggtctttt tccgttcaag aaactgaacg
    78481 aagaaaaggg aaagttgtct cttttgtgat aagagaaatc ataataaacg aatgacttac
    78541 tttccaataa attgagaaca gtcagaacca gacaaattag ttgcatctga tgaataaagt
    78601 gattattcta tgacatgagg tgatacagat gaaattagaa gtgacaaaag aagcacaaga
    78661 agtattgaag aataaattag aagcagacga tcaactttta ttagatatcg aaaacggtga
    78721 cggtccattt gcagaaagcc aagtcagttg ccaattggat acggcatttc gattgatcat
    78781 tgtcaaaaaa gatacacaaa cagatctaag tctttattct gtagtggctg atacaccagt
    78841 tggaccaatc aaaatcaaag acagtgcaat actttacatg gatgatccat ccacacttgc
    78901 tttagaacca acttataata gtttacaatt aaaaggacca agcggcttga gaaaaggcaa
    78961 cttgcaagtt gttcgaaaag actagaggga taaaatggga aaattggcga tcatcagcga
    79021 cctacatgtg gacatcaata aattcggcga atcagagctg atgcagcttt ggcaagtttt
    79081 gcaaaagcac aagatcactc ggcttcatct agccggagat acggctaata aagtagaccg
    79141 ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta tctacgacat ttcatttcgg
    79201 caatcacgag ttggcagatg tgcccaatga acagatgatg gaccattttc cagacccaca
    79261 ttttttaaat gaacggtata ttgctttaaa tgagcagacc gttcttttag ggatgaatgg
    79321 ctggtacgac tatcagtttt ccgaattgaa agattacgag gcaatcgtac gaatgaagcg
    79381 tttgtattgg tatgatcggt tcatccagcg tgggaaaaca gatctcgagg tcaatgcaga
    79441 aatgcttgca gctgcaaaaa atttgctaga ccagctggaa gcaaaaaaac ttgaggtgat
    79501 tttatccact catttcgttc ctaaacgggc atttatcatg tatcaatctg ctccttatga
    79561 gcgttggaac aaattaaatg catttttagg atcagaatca ttcggagaat tacttgaccg
    79621 ttattcaaat atcaaacaag tagtttttgg ccatacgcat cgtcgctttg aagataaatc
    79681 catcggtcac acgatttaca gctgcagacc ttttggttat tattatgaat ggcagctgac
    79741 ccgagatttt gtcttgaaag aacagttgct agacagctat gatcctatga aattacgtgt
    79801 cctcttgcgt gcccatcacc cagcttttca ggcctatcaa acacaacatt tgcaagagga
    79861 atttgaacaa gcgatgacaa tcgttccgta ttgagacagc attagtaatg tgtaaaagaa
    79921 aataccttgt aaaacataag gataagtaag agcagtagat ccatttatcg tggatatgct
    79981 gctttttttg cggaaaataa tcagcgaatg tttatttgat cggatgattt gtaactcgca
    80041 gagtactaga tgcaagcgtg tattcttttt ttgaacgata atcacaaaaa aaatataata
    80101 tacatttttt atttaattat aattaatatt gattgaagtc taggcagtca agtcacattt
    80161 tgatagatag gagggggact tacgaattat tttaaaggct atgacagctg tatttctaga
    80221 ttgtgtttgc atcaagttgc aataaaaact agaaatcaaa tcgggctgat aatcatttat
    80281 aactaatcga tacataggac caaatatagg tctatgttct tttatttcat aagtatcgta
    80341 aaacagaatt ttgctatgga ttaatccaat cgactgatat gtttagtaaa taaagaaaga
    80401 ggaatgtcaa tgatgaaaaa agaaatcgta ttaacagcag caataatgtt attcagcatg
    80461 gtagcaagta cgacttttgt ttctgcaact gaagtatacc caaaggaata taacacagaa
    80521 ggaacgatta catttgaagc aggagatgag ggtgtaactc ctcctgttga tccagagaat
    80581 cctgatccca ataaacctgt cgatccatca gatcctccaa gccctggaac aggaggggca
    80641 ttatcgattg attatggatc aaaattcaaa tttgggactc aaaaaatttc tactgcggat
    80701 aaaacgtatt atgcagctgc tgatgtgatg aacgatgggt ctagaaaacc gacatatgtc
    80761 caagtgacag ataggagaag taccttatct gggtggaaat taagtgtctc tcaaccagaa
    80821 cagtttaaaa ctgccagtgg ggatgaacta gttggagcac agctgaaatt tacgaaaggg
    80881 caggctgttt ctttggttga tccaacatat acgccgcaaa cggtgaattc tgaattaacg
    80941 ttgacaccgg gaggaaacaa tacgttagca attaatgcta aatcaggaac aggtgtgggg
    81001 acttgggtct atcgatttgg tgcaaatgaa aatgaaaatc aagatgcggt acagctttcg
    81061 gtacccggta aatctgtcaa gttagcgcaa caatattcca ctaaattagt ttggacatta
    81121 gaagatacac ctaacaacta gtttactgaa aagaactgtt aaaaataaat acgtagtcat
    81181 attgtcttgc gaggtaatcg agcaaaaatt gtcaaaggct aggctaaatt tgtttggata
    81241 cgaatagttc atctaggaat ttaacgatca ggaggaaaag atatattgaa aaagaggagg
    81301 atcgtgctca ttttaagcag tctcttagtg actaatatct ttccgatcat agggtactct
    81361 ttggaattga atcagttgga gcatggaaat agaagagacg aagaaagcaa actcacttcg
    81421 acattcgaca gtttaataga atccaaccag acacaagaaa gcagtgagga tttaagtatt
    81481 tcgagtagtg gagagaccac tgacaacgta gaagctgagg gttcggacaa acaaggacta
    81541 gaagatgcac caatttctgc ggttgagctg cctgacgatc ccaatattat ttctatcgat
    81601 aaaatttttc aaacacctat cggtactagc acgtccatat tggaaaatgg gaagttactg
    81661 cagttgaacc cagcagaaag gtctcaaaga ggagctattt ggtcaaataa gaagattagt
    81721 ttactctcgg attttacatt taaatcttat ttatacttag gaaatcaacg agatagagcg
    81781 ggagatggca tgacctttac attgactggc gatcctagaa tggctagtaa tcccagtcaa
    81841 gttattggat cgccaggtat gggaattggc tcatattcta ctcgttctgg ccaaccttac
    81901 attgcgaatg cgctctcgat tgagtttgat acgtataaaa atagtggttc ttctgatcga
    81961 atggatcgag agataactgc tgataataga gggcatggac atgtcgcttt tgtcacgcct
    82021 aaaccaaata ataataatta ttctggagag catactggcg tgacggtagc tcctacattt
    82081 ctttctaatg gcacatggcg cttgttaata attaaatggg acgcggagca aagaagtctt
    82141 acttatgatt tggaaggtgt cggtcgctct tctcggatta tacctgatct cattaggcag
    82201 tttggttcta cagaagtaaa ttgggggttt acttcttcaa caggtacttt ttttcaagaa
    82261 aatgctgtcg ctgtcacgcg acttccatcg aatgtcacat caacagctga tgtaaaagtc
    82321 aacgatagtg attatggggc catcgctgaa gctggaggaa acgataagat aactatcaga
    82381 aatacattga aggtcgaggg ggattttctt gaatataaga aaatgcagac tagtatcgac
    82441 ctcccacaag aattaactat tcccgagcat atcttgatca atggaaatca aattgatgcc
    82501 aaagatatct tgataaccgg aaatcgagtc acgctaaatc tgacagatta ttttattgct
    82561 ggagatacgt taataatcga attggagaca actttgcagg aagtaattcc tgaaaaagta
    82621 ctcactatgc attttgaata ccttgaaaac gggaatttga ttgagcaatc gaatgaaata
    82681 aacctaaaga tcgcaaagct gaaggagata gatatccatg tttattataa agataaagac
    82741 agtcagattg atattgctga aatgaaaaca atcactggga aaataggaga gaagtataaa
    82801 gaaactccta ttgatattga agggtatgtt tttgactcgg attcagcgaa tacagaaggc
    82861 atcttctccg aaaatacaga ggatatctat ttttattaca gaaaaggcaa actttatctc
    82921 tcaactgctc ctcaatatgt caattttgga aaacataaaa tatccagtag accattaagt
    82981 gaattcggac agttttcagg tgggttgaaa attatagatg aacgggcttt tggaagttgg
    83041 aaattacaat tgaaacaaga gaaagttctt acaaatggag attttgagct tccagaagct
    83101 ctttcctttg ttacctctga aaatacaacg gttataggga catccgctgt tacgattttc
    83161 gaaagtgatc aaaagggtga aagcgatttg tcctctcttt tagattctac agctcatcgt
    83221 ggaatcagaa tagatattcc tgtagaaaac caaagaatcg gaacctttga ggggaaacta
    83281 tcttggatac ttgcagatgt cccaggaaat taacaggaag agaagaatat aacaaaatta
    83341 ttgagtttca agaagggcac aggtaccata ggctcttgtg tcccacagag acaagatttt
    83401 gttatagtct tctcttacat atgattaaag gagcgatttt cttgagacca aaacaacaat
    83461 tatttataac actcctagtt attttagggg cactttttaa caatatatca gtgtcagcta
    83521 gtgcatttaa tttttcagtg acgccgataa cttcagagaa tcaaatcgac aagagaaaaa
    83581 cttacttcga tcttcaactt gatccggatc aggaagtaga agtaaaagcg gaattgagaa
    83641 atgatacaga aaaagaagtg aaaatcgata tttctgtgaa tagcgcaacc accaattcaa
    83701 atgtgatggt tgaatacgga aaaaacgaga ttgaaaaaga catgagcctg atctttgatc
    83761 tggtagatta tgtgagctat ccacagatag tgacgctaaa acctaaaagt gtccaaaccg
    83821 ttgcttttca tgtcaggatg ccgaatgagc gatttgatgg tgtattggct ggtgggatca
    83881 cattccaaga aatccagacg gaaaaggacc aaacagaaac taaggatcaa agcttatcga
    83941 ttgagaatga atacacctat attattgctt tgttgatgca acagacactg aacgaagttg
    84001 ctccgaacct tactcttcat gaagttaaac cagatcaaat caatgcaaga aatgtcatct
    84061 tagcaaacgt tcaaaatgat caaaaaacct atatcaatca agtagttatt gaaacaaaaa
    84121 ttacaaaaaa aggacattct gaagttttat atcaagagga aaaagaagga ttgcagattg
    84181 cgccgaatac aaatttttct tttccaactg ctttaaatgg tcaaccgcta accccaggtg
    84241 aatatcacct tacaatgact gttttaggaa atgaaaatga aaatggaaaa ttttcgagaa
    84301 aaaaaggaaa tactacgatc aactatacca atcaatgggt atttgaaaaa gattttacaa
    84361 tagacggaaa agtagccaag gaattgaaca cgacggatgt tacgattaaa aaagacaata
    84421 attggattta tatcttagta gcttcacttt tcttattgtt tattttatta tgcatacttt
    84481 tgtttgtttt gagaaaaaaa gaagagaaag aaaagtaagg ttaagataac aaatag
//
LOCUS       DAEGIQ010000002        59615 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000002 DAEGIQ010000000
VERSION     DAEGIQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.002"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            189..419
                     /locus_tag="KMT45_000090"
     CDS             189..419
                     /locus_tag="KMT45_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBE7003584.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="KMT45_000091"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="KMT45_000091"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBE7003585.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="KMT45_000092"
     CDS             1830..2006
                     /locus_tag="KMT45_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBE7003586.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="KMT45_000093"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="KMT45_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBE7003587.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="KMT45_000094"
     CDS             2622..3089
                     /locus_tag="KMT45_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7003588.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="KMT45_000095"
     CDS             3174..4058
                     /locus_tag="KMT45_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBE7003589.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4172..4408
                     /locus_tag="KMT45_000096"
     CDS             4172..4408
                     /locus_tag="KMT45_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBE7003590.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="KMT45_000097"
     tRNA            4496..4576
                     /locus_tag="KMT45_000097"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="KMT45_000098"
     tRNA            4583..4654
                     /locus_tag="KMT45_000098"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            4779..4949
                     /locus_tag="KMT45_000099"
     CDS             4779..4949
                     /locus_tag="KMT45_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003591.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5169..5876
                     /locus_tag="KMT45_000100"
     CDS             5169..5876
                     /locus_tag="KMT45_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7003592.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="KMT45_000101"
     CDS             complement(5935..6579)
                     /locus_tag="KMT45_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBE7003593.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="KMT45_000102"
     CDS             6705..6992
                     /locus_tag="KMT45_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003594.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="KMT45_000103"
     CDS             7100..7420
                     /locus_tag="KMT45_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7003595.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="KMT45_000104"
     CDS             7501..8814
                     /locus_tag="KMT45_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBE7003596.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="KMT45_000105"
     CDS             8811..9530
                     /locus_tag="KMT45_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBE7003597.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="KMT45_000106"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="KMT45_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBE7003598.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="KMT45_000107"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="KMT45_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBE7003599.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="KMT45_000108"
     CDS             join(13091..13162,13164..14192)
                     /gene="prfB"
                     /locus_tag="KMT45_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBE7003600.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="KMT45_000109"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="KMT45_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBE7003601.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14967..15860
                     /locus_tag="KMT45_000110"
     CDS             14967..15860
                     /locus_tag="KMT45_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7003602.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="KMT45_000111"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="KMT45_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBE7003603.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="KMT45_000112"
     CDS             17797..18654
                     /locus_tag="KMT45_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBE7003604.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="KMT45_000113"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="KMT45_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBE7003605.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="KMT45_000114"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="KMT45_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBE7003606.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="KMT45_000115"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="KMT45_000115"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBE7003607.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /locus_tag="KMT45_000116"
     CDS             21410..22168
                     /locus_tag="KMT45_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBE7003608.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="KMT45_000117"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="KMT45_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBE7003609.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23095..24126
                     /locus_tag="KMT45_000118"
     CDS             23095..24126
                     /locus_tag="KMT45_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBE7003610.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24123..24890
                     /locus_tag="KMT45_000119"
     CDS             24123..24890
                     /locus_tag="KMT45_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBE7003611.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24990..26558
                     /gene="liaX"
                     /locus_tag="KMT45_000120"
     CDS             24990..26558
                     /gene="liaX"
                     /locus_tag="KMT45_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBE7003612.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26855..27175
                     /locus_tag="KMT45_000121"
     CDS             26855..27175
                     /locus_tag="KMT45_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBE7003613.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27175..27525
                     /locus_tag="KMT45_000122"
     CDS             27175..27525
                     /locus_tag="KMT45_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBE7003614.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27692..28630
                     /locus_tag="KMT45_000123"
     CDS             27692..28630
                     /locus_tag="KMT45_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBE7003615.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28645..29475
                     /locus_tag="KMT45_000124"
     CDS             28645..29475
                     /locus_tag="KMT45_000124"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBE7003616.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29519..30544
                     /locus_tag="KMT45_000125"
     CDS             29519..30544
                     /locus_tag="KMT45_000125"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBE7003617.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30559..31497
                     /gene="galU"
                     /locus_tag="KMT45_000126"
     CDS             30559..31497
                     /gene="galU"
                     /locus_tag="KMT45_000126"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBE7003618.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31622..32062
                     /locus_tag="KMT45_000127"
     CDS             31622..32062
                     /locus_tag="KMT45_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBE7003619.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32065..32667
                     /locus_tag="KMT45_000128"
     CDS             32065..32667
                     /locus_tag="KMT45_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBE7003620.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32726..33832)
                     /locus_tag="KMT45_000129"
     CDS             complement(32726..33832)
                     /locus_tag="KMT45_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBE7003621.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34042..35043
                     /gene="ccpA"
                     /locus_tag="KMT45_000130"
     CDS             34042..35043
                     /gene="ccpA"
                     /locus_tag="KMT45_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBE7003622.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35380..37725)
                     /locus_tag="KMT45_000131"
     CDS             complement(35380..37725)
                     /locus_tag="KMT45_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBE7003623.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38004..38906
                     /locus_tag="KMT45_000132"
     CDS             38004..38906
                     /locus_tag="KMT45_000132"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBE7003624.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39119..40894
                     /locus_tag="KMT45_000133"
     CDS             39119..40894
                     /locus_tag="KMT45_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBE7003625.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40960..42546)
                     /locus_tag="KMT45_000134"
     CDS             complement(40960..42546)
                     /locus_tag="KMT45_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBE7003626.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42797..43021
                     /locus_tag="KMT45_000135"
     CDS             42797..43021
                     /locus_tag="KMT45_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBE7003627.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43163..44914
                     /locus_tag="KMT45_000136"
     CDS             43163..44914
                     /locus_tag="KMT45_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7003628.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44914..46698
                     /locus_tag="KMT45_000137"
     CDS             44914..46698
                     /locus_tag="KMT45_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7003629.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47024..47875
                     /locus_tag="KMT45_000138"
     CDS             47024..47875
                     /locus_tag="KMT45_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE7003630.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     EKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47929..49680
                     /locus_tag="KMT45_000139"
     CDS             47929..49680
                     /locus_tag="KMT45_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE7003631.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49684..49854
                     /locus_tag="KMT45_000140"
     CDS             49684..49854
                     /locus_tag="KMT45_000140"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE7003632.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49894..50796)
                     /locus_tag="KMT45_000141"
     CDS             complement(49894..50796)
                     /locus_tag="KMT45_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBE7003633.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51027..52430
                     /locus_tag="KMT45_000142"
     CDS             51027..52430
                     /locus_tag="KMT45_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBE7003634.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52430..53863
                     /locus_tag="KMT45_000143"
     CDS             52430..53863
                     /locus_tag="KMT45_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBE7003635.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            54008..54934
                     /gene="trxB"
                     /locus_tag="KMT45_000144"
     CDS             54008..54934
                     /gene="trxB"
                     /locus_tag="KMT45_000144"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBE7003636.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55148..56875
                     /locus_tag="KMT45_000145"
     CDS             55148..56875
                     /locus_tag="KMT45_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBE7003637.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56890..57240
                     /locus_tag="KMT45_000146"
     CDS             56890..57240
                     /locus_tag="KMT45_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBE7003638.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57332..58192)
                     /locus_tag="KMT45_000147"
     CDS             complement(57332..58192)
                     /locus_tag="KMT45_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBE7003639.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58284..58712
                     /locus_tag="KMT45_000148"
     CDS             58284..58712
                     /locus_tag="KMT45_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBE7003640.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58874..59050
                     /locus_tag="KMT45_000149"
     CDS             58874..59050
                     /locus_tag="KMT45_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBE7003641.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59077..59523
                     /locus_tag="KMT45_000150"
     CDS             59077..59523
                     /locus_tag="KMT45_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBE7003642.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        19421 a        10337 c        12778 g        17079 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atctctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaagcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgac gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagtttc aggaggtctc tttgacttag atcagctgga agaagacatc gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgacagcga agccgcacaa aaagtgatca
    13261 gtgaaaataa cgctaataaa gaaacctatg accaattcca ttctcttgcc gaagaactag
    13321 aagaaatcga attgatggtg gagatgctgc aagaagaacc tgatgcagca atgcaagaag
    13381 aagcagaaac acgtatcaat gcattagatg aaaaaatgaa gacgtatgaa ttatctatgc
    13441 tgttagacgg accttatgat cgaaacaatg cgatcgtcga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagactgg ggcagcatgc ttttacgtat gtatacacgt tgggcagagc
    13561 agcatggcta tcaagtagaa acactagatt atcaagcagg tgatgaagca gggatcaaga
    13621 gtgtgaccct gctgattaaa ggacataacg cttttggtta tctaaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacggatt tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gctcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatcccta ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct cgattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gagatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tcgtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacgttgact ttagtcggtg tattcatggc agttatcatg aatgcaacta
    15121 aattagctca ggatatagaa ggaaacgttg atgtatctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagtaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaggaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat ggctgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaagcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaaatc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatatctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctgatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactgatt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcatttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacacaaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgaggattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgtg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta ttgttgccac accatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggacag ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actgtcacaa cgatgactgt tgatgcatta
    19321 aaggcagtac cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggacttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatgtagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctgacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcattgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggctttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggtttca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgatcat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcggctcg tatcagcgac tatactgcct ttttctatct
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 aattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagt ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cgggagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaaaat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 taaagcggtg attctgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa atagctcctc atatttttca
    22981 tttggctgga cttattaccg cagatgcaag cttttgaaca ctgtttattc gggaaagggt
    23041 ttgtgacaaa atatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa
    23101 aaaaaaatta gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag
    23161 catcaagctg ctttatatga agtacctgtc atcgagatcg aaaaagtacg aataaaagag
    23221 gatcatcaat tgattacagg aagactgatg aacacgaatc aaaaaggaga atcgatttat
    23281 ttatcggttc cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg
    23341 tatctagtcc aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg
    23401 cttttcttct tcacaagtat cttcattggc gccatgttgc ttattggcgg aaaacatggt
    23461 ctatttgctt taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc
    23521 tttactaaaa tagaagggat ttctctatta aatatatcta tcctgttcgt cgttataggg
    23581 atgattatta gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca
    23641 gctgcagtta cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatctttgg
    23701 aaagaccaag gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt
    23761 tttctttcta gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc
    23821 attgcgacat tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg
    23881 ctttcgggaa ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc
    23941 gtctatatca gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtcgttc
    24001 aaagaaacga tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta
    24061 ggaatagtat tatcgatccc tatctcggtc ctcttttttc tgactgtaag gaggctgaaa
    24121 cgatgaatgt acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg
    24181 gtacgcaagg tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcttgt
    24241 tgttattcgg ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat
    24301 tgattgctgt ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt
    24361 ttgtgagtat actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt
    24421 tagcaatcca aggttttaca acgatagaac tagaagaatt atctggctta gataagactt
    24481 tagcgattga ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg
    24541 ttttagatgc gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc
    24601 aattatcttt ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt
    24661 caacagtaat gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt
    24721 ttattgattt agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat
    24781 atacgatggc tattcttacc acattgtccg cactacttgt tcttccttta acctgcgtga
    24841 ctacagcata tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac
    24901 ttaagtccta tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc
    24961 aaacaatttt gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag
    25021 tgaaaaaagg aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg
    25081 aaaaagacga aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa
    25141 aagatcaggc aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc
    25201 cagttgatcc aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg
    25261 aactagcaac tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg
    25321 ctgacaataa ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag
    25381 aagaattagg cgagttgaca gaagaagaat tagcacagcg tcagacgtta gaagcagaaa
    25441 tcaaagagtt agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag
    25501 aattaaaaga tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt
    25561 ttgaattgcc agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa
    25621 aaatgtcaga agctggatca cagttaggaa aattcttgaa gaaaacattc caaactgttt
    25681 cagaaacagt gaatgacaac atggaatgga aagatgtcag cttgcgtgtt cctgggattg
    25741 cgacaacgaa atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata
    25801 tcaaggcagc aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg
    25861 aagctaagat caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac
    25921 ggagccagat tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc
    25981 gtgcagacct tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc
    26041 taaatggcaa catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga
    26101 atggtaacat catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg
    26161 gcaatatcga tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta
    26221 cagtgacatt tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat ggtgatgtac
    26281 gtcttacgat taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg
    26341 tcaaagtcgc tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa
    26401 gtatcaacag ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc
    26461 aaatgttgca attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa ctttcaacga
    26521 caacaggaag tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc
    26581 agccttgagc aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc
    26641 cttattgccg ataggctatc tcttgaacac cgtttaccaa gagattatga acttgccaat
    26701 tatcgccgag tcacaaggaa taatttcaaa aaaagctcgt cacgttttat gaatatggta
    26761 atttgtgacc gagaattttg caaaaagaca ccgattaaga agaaggttgt cacaacgatt
    26821 ttcttcattt aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc
    26881 caataaaaat gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga
    26941 tccgactgtt atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat
    27001 cattttatac attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta
    27061 tggacataac aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac
    27121 aaaaaaacgc aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg
    27181 tactttcaac gtattttagt taatacgtta acattcatat cattggctgt tctttttcct
    27241 gcgatggtcc atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg
    27301 ttgaatatgt ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca
    27361 ttgggattgt ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg
    27421 ggagcagaac attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg
    27481 tcgcttgtca atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg
    27541 aactatcggc gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat
    27601 taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag
    27661 agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt
    27721 agtgaaaaaa ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat
    27781 cgtaactggg gacatctcac gcccaggact agagttgact ggttatttta attattattc
    27841 ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc
    27901 agaagagcgt ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat
    27961 ttcaagagga ttaccagctc cagaagaaat gatccaggca gcaaatgaaa atgggatagc
    28021 tgtactgcgt tctcccatct caacttctcg cttattagga gaattatcca gttatttaga
    28081 tggtcgcctt gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg
    28141 tgtactgatt caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa
    28201 acgcggtcat cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac
    28261 ggtgatcgga gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat
    28321 cattgatgta atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt
    28381 ggctgtttat ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga
    28441 cacgaccgtt gaaatcgcgg aagtaggcat tccacaaata aaaatccctg tcaaaacagg
    28501 acgaaacgtt gcaatcatca ttgaagtagc cgcgatgaat ttccgtgcac gtacaatggg
    28561 ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg
    28621 caacgactag caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt
    28681 gtagggattc ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgccatg
    28741 tggcttagca gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt
    28801 atgttgtggg gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat
    28861 cttccccagt atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct
    28921 atttatggcg gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt
    28981 atttctttgt ggactttttt agatatcgct gcaccaagtg tattattagc acaagcaatc
    29041 ggtcgttggg gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc
    29101 ttagaaagtc tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat
    29161 cgtcaaccaa catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg
    29221 tttttacgaa agaaaccaca ccttttgaaa aaaggtgaag tccttttatg ttatctaatg
    29281 tggtatagtt tcgggcgttt ctttattgaa ggattacgta cagacagtct ttacttttta
    29341 ggtgtgatcc gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta
    29401 tttatttggc gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat
    29461 caacaaatta tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat
    29521 gaattataga gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct
    29581 cgccgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat
    29641 caacacccaa cacacgaatc aacgttattt accagatcat tgtctacccg attccatcca
    29701 agcattcacc aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc
    29761 aacgaaagca atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt
    29821 gatcatccat gcaagtaaag gattagaaca ggggacacat aaaaggatct ctgatatctt
    29881 gttagaggaa atcccagtag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca
    29941 tgcagaagaa gtagcgaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa
    30001 agcagcaaaa tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc
    30061 tgatgtgatc ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg
    30121 cgctattcac ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt
    30181 agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt
    30241 aagcggagta ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc
    30301 aggcaacttg ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt
    30361 cgttgaaggt gtgaatacta cccaagcagc gatggaacta gcccaaataa tggaaatcga
    30421 aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc
    30481 cgcaaaagaa atcatgttac gtgatgggaa aaaagaaaat gaattctcat taacaatttt
    30541 ttgaaggagc gaatgtatat gaaagtcaaa aaagcagtta ttccagcagc aggattagga
    30601 acaagatttt taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa
    30661 ccaacaatcc agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc
    30721 gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa
    30781 acaaacctaa gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat
    30841 gtcaacttgc atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa
    30901 gcaagagcct ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa
    30961 gacgaagtac cattgacaca gcaattaatc gatgattacg aacgtactca tgcttctacg
    31021 atcgccgtta tgcaagttcc tcatgatgaa acttcaaaat acggaattat caatccagga
    31081 gaagtattgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc
    31141 aaagcaccaa gtgatttagc tattattgga cgttacttat tgactccaga aatttttgat
    31201 gtattgtccg agcaacaacc aggagccgga aatgaaatcc aactaactga cgcaatcgat
    31261 acattgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttacgatgtc
    31321 ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctaacaca tccagaagta
    31381 ggtgcacctt tacgtgagta catcctgtct ttaggtaaaa cattagaagc agaaggaagc
    31441 aagatcgatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataaccc
    31501 ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt
    31561 tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat
    31621 catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt
    31681 ttttatcgtt cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag atcagactgt
    31741 agcagaagca aatacgacga tcgaaatcgt cacaaaagac gtagatcttt tgtctagaca
    31801 agtagaaggc ttactggtca aaagtaatga actgctcttg gatatcaatg gaaaagtcgc
    31861 aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat cagaacttaa
    31921 tcattctagt aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga cagctaaagc
    31981 gacggttgcc ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca aaaaacatac
    32041 tgaaacgaca ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt aggtgcagta
    32101 gtcggaggca cagccgcagc aatcgccgcg ttattgttag caccaaaatc tggtaaagaa
    32161 cttcgggatg aattagctgc ccaagcggat gatttgatgg atatggcttc agattacaca
    32221 gatatggcga aagaaaaatc gactgagtta acaggtattg caaaagaaaa agcgcaagat
    32281 ctttctcaac aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag tgaatcagca
    32341 gatgaagcag gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca atcagctgac
    32401 ttatctaatc gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga tgaattagga
    32461 agtattgcta aagatacttc agaagatatc atgattgatg taaaagacgc tgctggtaaa
    32521 gcaggagata ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga tgactcaaaa
    32581 gaaatgatga aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat aaaagataca
    32641 atgacagatt catctacaaa tcactaaaat cacaaattta tagaataaaa aggtgggagc
    32701 ggactagctc tcaccttttt tactcttatt tttctaaaac aagtaattct tttggtgtag
    32761 tcgtgaatgg cacacagcca tcgcttgtca catatacaca gtcttcgata cgtaccccta
    32821 ctttttcagg aatatagatt cctggttcaa gagagaagca catcccttct tcgattacta
    32881 agtcatttcc ttctacaagg gaagggaatt cgtggacggt tgttccgatc ccgtgaccta
    32941 aacggtgatt gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat
    33001 caagttcacc tgctgtcaca cctggacgga cggcttcttg tgcggcaagc tgtgcttcaa
    33061 gagtgatgtt gtagattttt tcttggaagt cggttggttt ttgataagca attgtacgag
    33121 tagcatcact gcaatagcca ttccaaacaa cgccaagatc gaataacaca agttcattcg
    33181 gtgaaatctt cgtatttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga
    33241 ctaatgtgtc gaaagacatt tgtgaaacac cttgtttttt caattggtat tcaatttctg
    33301 caatgatcgc ttgttcagtc acgccttctt tcactgcttt aaagccaatc tcaaatgcaa
    33361 catcggccca tttaccagct tcgatcagtt tgtctttttc ctctagagtt ttgatcagtt
    33421 gtagacgttg gatgatggaa gttagatcag tgtaagaatc cgttcccgtc aagatatcag
    33481 agatagcatc gaagcgctcg acagtcaagg cgttcttttc aatccctaca ttgccaggct
    33541 gaccatagcg agcacgaagt tcttttccga tgatctccca agggttttcg ctgtctaaat
    33601 agccacggac atcatacgcc caactactgt tttgagcatc ttcaacttcc aaagcaggag
    33661 tgaaaaggaa aggatcttgt tcaagaggga taaaaagagc tagtacacgc tcatgaggat
    33721 cactttcgta tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcga
    33781 cttggtgttg tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac
    33841 ctactttcta aattaatttt gtattcaata attgtaccac tgatttatta aaagaccaac
    33901 gttctaaaaa ctgaaaaaag taaaaatttt catgaaaaca gagataaaac ggttgcaatt
    33961 cttgagatta tttgatattc tattttagaa atgtaagacg tattttcaga aaacatataa
    34021 aatatgaata ggagaacaca aatggaaaag caaacaatca ctatctatga tgtagcgaga
    34081 gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc aaacgttaaa
    34141 ccagcaacaa gaaaaaaagt cttggaagtt atcgatcgct tggattatcg tccaaacgca
    34201 gttgcgcgtg gcttggcaag taagaaaaca acaacagttg gtgtaatcat ccctgatgtc
    34261 agcaatatgt tctttgcttc tttagcacgt ggtatcgatg atgtggcaac aatgtacaaa
    34321 tacaatatta tcttagcaaa ttcagatggc aacgaccaaa aagaagtcaa tgtattgaat
    34381 aacttactgg ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat cacagacgaa
    34441 atccgtggag aattttcacg ttcaaaaact cctgttgtat tagctggctc tattgatcca
    34501 gatgaacaag taggtagcgt aaacattgat tatgtatccg caacaaaaga tgcagtaaac
    34561 aaattggcaa aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt gatcgatccc
    34621 atcaatggac aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa taacctacca
    34681 tatagcgaag gattgatctt tgaagcacaa tacaacttca aagatggtct atctctagtc
    34741 gatcggatcc ataatagcgg tgcaacagct gtctatgttt cagatgatga attagccatc
    34801 ggtattttgg atggtttatt agaccgtggc gtgaaagtac cggaagaatt cgaaatcatc
    34861 acaagtaaca attcactact gacagaagta gctcgtcctc gtttaacaag tatcacacaa
    34921 ccattatacg atttaggggc tgtatctatg cgcctattga cgaaaatgat gaataaagaa
    34981 gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg atctacaaaa
    35041 taataaaagg acaaccattc agaggttgtg tgcctgccgt ttaactctgc gttacaagga
    35101 gcaatttcaa aaaacagcat ctcatgtttg atgaatattg tgactcgtga ttcggagttg
    35161 gcaggagaac accgtttatt tgggaaaagg ggttatgaca aaattttgtt ataatccttt
    35221 ttttgtgtct taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat
    35281 ccgtgtatag atgctgcatt ttgttcacca ccatcaaaaa aataagggac tgtgacaata
    35341 ctattgtcac aatcccttat tactgtgtat tctattaaat caatcatctt tctcatctga
    35401 ttctttagtt gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata
    35461 gttttcatcg gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc
    35521 ccatagcgat tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt
    35581 tccaggtgtt tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc
    35641 gaatttctca tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt
    35701 ggcaagataa ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg
    35761 ccctgcccag ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta
    35821 ttcattggta gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct
    35881 tgagatggca tctttgaatg gtgtagtgat ttttgcattg atcacactac gcatcatgtc
    35941 attcatgatc gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata
    36001 aattacattt cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc
    36061 gccgtttgct aaagcctgaa atccgttggt ctgttgaagc gtcgtgacat ctactgtacc
    36121 taaaggagca gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta
    36181 gtgctcgtaa gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata
    36241 agccgggata ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc
    36301 ttcgttagta gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc
    36361 ataatctgat acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac
    36421 aagaactggt ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg
    36481 attctgactg taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat
    36541 caatacattt ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt
    36601 ttcaacgcca ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata
    36661 tcctttattt tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc
    36721 agacatatca gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt
    36781 catcacagta taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa
    36841 gtcttttgta aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc
    36901 acgatacata ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt
    36961 tagcgcgcct gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat
    37021 ataagcagct tgcggcagcg tcacatcttt tgcatctaca ccaaagattc cttttgcagc
    37081 ttcctggact ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa
    37141 atacgtagca atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat
    37201 ctcgtttgct ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg
    37261 ctgtgtcaaa gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg
    37321 taaaacagct ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat
    37381 aattgcagtt ttcaataaag gtgaaatctt atcagatgaa atagtcgtgc gcatcagatc
    37441 tgtctggatt tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat
    37501 gtttcctagt tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata
    37561 ggcaaaatag ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt
    37621 aataaaaaca gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc
    37681 tttttttctt ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac
    37741 taatggtttt atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt
    37801 tttggcagga atttcttttt taaaagagtt aaaacgtgag aaaatgatct ttattataaa
    37861 agagttctta agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa
    37921 aaatcaatca ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt
    37981 gcctttcaag gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga
    38041 aaatgctgtt aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct
    38101 atacaggcgc ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg
    38161 gaaaagcgta catggcagaa aaaggtctaa caaatatcgt cgttcatgcc ccttatatca
    38221 ttaatttggg gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag
    38281 aagagattgt tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc
    38341 atgtaggggc aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt
    38401 tgacaaaaga acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa
    38461 tcggtcgaac atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat
    38521 tatccgttac ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact
    38581 tttcaggtgt aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc
    38641 atatcaatga ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt
    38701 ttggaacaat cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata
    38761 tttcgaaaat cttggaaact ccctatgtag gcaaagataa aaagaaagct tacgcaccat
    38821 atggaaaaga aatcgccatg ttcaaagcgc aaacattcga tccagaagtg tttcctgagt
    38881 tattagcaga agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag
    38941 gcacattgcc ttgtcctttt ttgtttaaga aacaaaggag tactttttgc agtctttttc
    39001 atcatgcatc ttctcccccc tctttttaaa tttagacaaa caaggtatac tggtagaaaa
    39061 gtaaaaaaac gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat
    39121 gctgaacact ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct
    39181 tggcatagcc ctattattta caattattcg tttgtattta tatagtaaag caatctatgg
    39241 aacagacatg tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt
    39301 ggacggtaaa tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag
    39361 cttattttta gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt
    39421 cagtattttt gccagtatat tggttgcact cgcgtttaaa ccgattgtga agaacaagta
    39481 tcttctttta agtatctatt tttatttttt gttttctccg attagtttta cacatgaata
    39541 ttccactcga acatatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg
    39601 tttattagct atttattacc gcaaatacgg ttctagcaga aaattgctgc catggttttt
    39661 agggctttct gtcgcttttc ctttcttttg gttcattcgc gaagattcta tctggcttct
    39721 tccttttact gtagtagctt tgggtatcag tttcgtccag atcttgctag ggcaagactt
    39781 ttctttcaaa gaagtaaaga agttaaaaaa acgactcttt cttctctcga aacgggaatg
    39841 gctgaaactt tcattgtttt tacttcctat agttggcttt tccatcgtct atcaatggat
    39901 cgaatataaa aatgagacaa catacggtat tgcaaccatc aatgaccgtt ccagtggaga
    39961 atttggacgt ttgatgaaac aactgatccg aatagatgat gaaacgaact tgaataaaga
    40021 aaacagtgag atatgggtct cacatgctgc actaaataaa gcattagccg catctcctac
    40081 attccatact gtttctaccc gtattgacga attatatacg actcacgcgt ggactcaagg
    40141 tggaaaagta aaagaattac ccggagatat catcttttgg gcattgcgag atgtggtaag
    40201 cgaatctggt tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa
    40261 ccaagaatta gaagaagcct atcacaaggg aacaatcaaa aagaaaaaag agttctatct
    40321 gatgggatca ggagatggaa agacgatgag tgatctgcca atcgtattcg atttccttca
    40381 atcaggatac gcttatgcat ggcgatataa agatttccat caaggaggaa attatagttt
    40441 cggaacgcaa gaacaagtca aacaggctca agaattatta aaccttccgc taatggagaa
    40501 ttggatcgat agtaatgaac cgagaacacc taatttccaa ttaacaaaat cagcaaaagt
    40561 cgcgaatgtc atcataaaaa tttaccaagt tctggctatt cccgtgctga ttgcttcaat
    40621 agcagggata ttacttatga tactaggaac cgtatttgca aaagctgatc gtgcctatta
    40681 tagttcctct ttgattgtgg tcaccggatt aatactgaca caattggttt ttcttttcgg
    40741 tgtctcatgg ttttgttctt atgcgccaga aaaaaaggac tattttttaa gtgtctacac
    40801 tggtgcaggc gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa
    40861 attaaatctg aaaaaagctt tatcttctaa ataaaaagac ccttggtagc tagattttta
    40921 acctaactat caagggtctt tgtgtggaaa aatcagtttt tattcgattt cttctaccgt
    40981 ttcctgcaaa agctgttcat attttttgtt tcctttatat aaatcaccaa tcgtccatgc
    41041 caacaggaga aggacaagaa caatgattac ataagcgatg tgatcagcca atgctagttg
    41101 agcggaagtg gcttgctcgc cgaagaaact ttcgatctgt cctggtaatc ctcgtaagtt
    41161 caagtaagtt agactaatca cagagaacca gccaagaagt tttacgatga atgaattttt
    41221 aaatcggcta cccatctccg cttggctgtt ggtcatcatc agtaaaggaa tcatcgaaaa
    41281 cggcaacgca aaagccaaga acacctggga ttcattcatt aagttattca aagcggcatg
    41341 ttcttcgatc gttccttttg tacttgtaaa caaaacacaa atcaatacag gaatcacaga
    41401 aagcaatcgt gtgaccagtc ttcttagcca aatcgggata cgcatatgga tgaatccttc
    41461 catgatgact tgaccggtca acgtaccagt aatagtagag ttctggccag aagcaagaag
    41521 tgctacagca aataaagtgg acaagatacc agattttgct acagcaatca aaataccatt
    41581 gctcatagct gatggatcag ataaagcttc atacagacca aaaaaggaag gatcttggac
    41641 agaaccggtt ttgaagacag cgacacccat gatcaatagc agtgcattga ctagaaaagc
    41701 cattgacaac tggatattag aatcccaagt agaaaaacgt acagcttgtg ctacatcttc
    41761 ttcgttttgg tgatcgatct tccgagtttg tgaaactgct gaatgaagat ataagttatg
    41821 cggcatgact gttgcaccaa taatccctaa agctccagtc agcggagttt cgccgccgat
    41881 gaccggtgag tctgagaatg tatgactgtt agggataaag ccagcaaata aactgtgcca
    41941 atctgggtta gacaagccaa cttgatagac aaaaacgaat aaaatgacga aaattaagca
    42001 gacaaccagt gcttcgatct tacgaaaccc gacttttgtc agtagtaaga gaacgaaaac
    42061 atctaataca gtaataaaga cggcaatcac caatggaata tcaaaaagta agtagagggc
    42121 gattgctgca ccaatgactt ctgcaatatc tgttgccata atggctaatt cagtcaagat
    42181 ccataaaatg acacctaatg tcttacttgt gcgtgctcgg attgcctgtg ccaaatccat
    42241 ttggctcacg attcctaatt ttgctgccat atactgtaaa agcatagcaa tcagactaga
    42301 catcaaaata actgacatca ataaatattg gaaattttgt cctccggtga tcgaagtaga
    42361 ccagttaccc gggtccatat acccaactgc tactaacgca ccaggtccag agtaagcaag
    42421 aagtgtttta aaaaaacttt tcccctttgg tacatcaact gttccattta cttcagctaa
    42481 agataaacca tttgtatgtt caataagtcg atgcttatga aattgtttat tggattgatt
    42541 attcaagaaa aaaccacctt tcctatttat ctacgattac attctacacc tcaaaaagaa
    42601 aaaattaaag aaaaagattc ggtacgccta attttttttc atggcgcgtc ttgctagatg
    42661 acgaaagaaa ttttgttctc ctctaaaaaa accactattt tttgcatgaa actaaagaat
    42721 acgcctttct tttttcaata aactttagta aaataagtca ttgagagcaa ttcggaaata
    42781 gaaaggagag atttatatgt tatcaacagg tattgtcgta cttattgcta ttattgcttt
    42841 gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga
    42901 aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa
    42961 accatctgag aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata
    43021 ggttctgacc caacgacttt ttacgggctg attttttcag tccgtttttt atttcgtaat
    43081 ttgttagact attcagatta ttcagcaaaa aagacaagca cagacaatcc taggctcttg
    43141 ataaagacaa aggaggagta aaatgtcgat ttttagaaaa ttaggctggt tttttcgtca
    43201 ggaaaagaaa cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt
    43261 agttcctcca aaagtgattg ggatcatcat tgatgagatc gccgataaaa atttatcaat
    43321 aaagatcatt ttcgggtgga tcttcctatt gattatcgct gcttttttac aatatctttt
    43381 tcgttatatc tggcggacga atatttgggg aagtgcagct cgattggaga aagaacttcg
    43441 gcagcagtta ttcaaacatt ttacagaaat ggacaatctc ttttaccaaa aatatcggac
    43501 tggggatttg atggctcacg caacgaatga tttgaatgcg atccagaatg tagcaggagc
    43561 tggtatcttg acctttgcag attcagtgat tacgggcgga acaacgatca ttgcgatgat
    43621 tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt
    43681 cacttcacga gtgctggggt caaaactcca tgatgctttt cgtgattcgc aagaagcttt
    43741 ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt
    43801 tggacaagaa aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa
    43861 aaacaaacga gtcaattttt tagactcctt atttgatcca ttcattacct tgattatcgg
    43921 tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc
    43981 gatcgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc
    44041 gattggtcga ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga
    44101 acttttgcat gaaaagacgc atatcgtgga acaactgggt gcagtgcaga ccccggcaaa
    44161 aggcgagctg aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt
    44221 agaacaaatc aagtttgcga tccaagaagg agaaacatta ggtattgttg ggaaaactgg
    44281 tgcgggaaaa acgacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa
    44341 aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat
    44401 cggctatgtg cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt
    44461 ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa
    44521 caatgaaatc aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc
    44581 tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga
    44641 attattaata ttagatgatg cactctctgc agtagatgcc aaaacagaag aagctatctt
    44701 gcataattta aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag
    44761 tagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg
    44821 aacccatcat gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca
    44881 attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg
    44941 acaaaatcga ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc
    45001 aaacctttta gaagaacgtt catcgttgct atcttatttg cgtttgcttt gtctgtgatc
    45061 aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg
    45121 gctacgacac aagttatttt gttttttgct ggtatgtact tatttggagt catagtaaaa
    45181 agtatcattt ggtttttcca atggtatttg tattccatgg cctcattgaa gacctatcag
    45241 tatattaggg taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag
    45301 acaccggcag gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt
    45361 tggtacgtct ttttgatggt attgactggg atctttgcag tcgtttcttc attccttgcc
    45421 atgtttcaaa tcaatggaga aattgccttg tggactttgg tgtttttgcc gattttagga
    45481 atcgtcatct ggtattacca gaaattcagt tctcgtattt accgaaatat gcgggaaaaa
    45541 ttaagtcagc tgaacacgaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag
    45601 tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac
    45661 acgcgttatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta
    45721 tatgcattgg cgattgcgtt ggtgttgact ttgtttggga tcgatgcgtt gcattctccc
    45781 gtagaagttg gactgatcta tgcatttacg acctatgtcc agggattttt taatccaatg
    45841 tctcaaatga tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc
    45901 ttgaagatta tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg
    45961 attacttcag gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat
    46021 gtattaaaca atatctcttt tgttgctaat ccgggagaga ccgttgcatt agtgggacac
    46081 accggaagcg gaaaaagctc gatcatcaac gtattgatgc ggttttacga attttatgaa
    46141 ggtcagatat tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag
    46201 aaaatggggc tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt
    46261 cggatgatga atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa
    46321 gcaaacaaat tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt
    46381 gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat
    46441 cctaaaattc tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg
    46501 atccaagaag gattggcaaa aatgcgtcaa ggacggacaa cgattgcgat tgcccatcgg
    46561 ctgtcaacga ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa
    46621 cgcgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg
    46681 caaagcagtg gtatataaaa gatgttgtga cagtagaatt ttgacctaag cactagagtg
    46741 aaaataaaac atagcttctc atatttttca tctgtgagag ttagtgtcgt agtcaccaga
    46801 tttaaaaaaa gattcgacag ctagtcagaa gtgcctaata gccactaaga aagcgttgac
    46861 aaaagcattt gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga
    46921 tgcaagctaa acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt
    46981 ttgttttgga tacagcccta tacatcatgg aaaggagcaa aacatgctga ttgagaaagt
    47041 actgaataac aacgtcgtaa taaccaggga tgaaagaaat caggaaatga tcgtgatggg
    47101 aaaaggtttg gcattcaaaa agaaagtggg tgacccgatt tttgactcat gcatcgaaaa
    47161 aaaatttcag ttgacaaatg acgggctttc acaacagttc caagaattgg cacaagcgat
    47221 tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctaca
    47281 agtccagttg aatgattctg tgtatattgc attgacggat catttgtata cggctatcga
    47341 acgtcacaaa atgggaatat ttgtaccaaa tgtattgctg tatgatatca aacaactatt
    47401 tcctaaagag tatgccatcg ggaaaaaaac agtgaagaaa atctcggata aataccacat
    47461 caagttatca gaagatgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat
    47521 tcaaagccag caagacaata cctacctgtt tactataaca atacaggata ttctaaatat
    47581 cgtccgctat tactataaga ttacatttga tgaagattca gtccattttt ttcgattcgt
    47641 cacacactta cgtttttttc tgacacgcgt cctagatgga gaaaaatcag aagtgggaga
    47701 agtttctgag gaattgtttc aggttattca aaaaaaatat aaggatacgt ttggttgcgt
    47761 tgagaaaata gcagactttt tagccgagac gttcaactac cagatgtcca cggacgaaaa
    47821 aatgtattta acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaaaaaa
    47881 ggaggataaa ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct
    47941 tccactaata aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc
    48001 gtagaaaatg tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt
    48061 cgtttcaaat taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt
    48121 attgtaacgg taatgcgagc gggagggcag tatcaagttg tcattgggaa tcatgtgcca
    48181 gtcgtgtttg aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca
    48241 gaaaaaatga gaccgtttga tatgcttatt gatggaatat cgggctgttt ccagccattc
    48301 cttgctatcc tagctgctgg cgggatgatt cgtggactga cagcattcct tgtcttctta
    48361 ggcgcgtttg accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta
    48421 ttccaattta tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa
    48481 ttcgtaggga tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg
    48541 agtggtgcag cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct
    48601 atttatcaaa cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt
    48661 cccattatct ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta
    48721 cctgagatga tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta
    48781 ggttttttac tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt
    48841 gaaacacttt tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa
    48901 attctggtga tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct
    48961 gtgaatggtt ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca
    49021 gcacttaccg cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca
    49081 gccattgttt ctggtattgt ggggattaca gagccagcta tttatgggtt tacattacca
    49141 cgtaaaaaaa tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg
    49201 ttaatgaatt taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat
    49261 attagaccag atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg
    49321 atggtggtat cctttacgct gacttttttc ttttggaagg atgaagcagg tgaaacagat
    49381 gatatacaga aaaaatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct
    49441 ccactaaatg cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg
    49501 atctaccctg aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gaccctgttc
    49561 ccgacgaaac aggcaattgg aatggtttct gaaacagggc tggaattgct gattcatgtc
    49621 ggattggata ctgtgcagtt ggaagggaaa tattttgaaa gcttagtgca gcaaggataa
    49681 agagtgaaac aaggcgatct gttgctacgc ttcgacaaaa agaaaatcga aaaggaagga
    49741 tacagtttag aaacacctgt gatcgtcacc aattacacgg attatttgga tattttggaa
    49801 aaccaagggg agacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt
    49861 aggtgaagaa tggactaggc ttaactattc tttttattcc acttctttat tttcaaaatt
    49921 gaaaggctct ttcatgatgg ctgagctgat agggcgttta tcgaattttt gaccgacaga
    49981 aattagatgg actaaattct gttgataatt ttctgcaaaa acacaactat ataacacgtc
    50041 tagaagataa caaattgatg tattgatcgt aaaattacca atttttgaat acagtcgctc
    50101 gcgtgtcgta agtcgcaaag cggctgtact cattgcagat agcgttgaat caccgatact
    50161 ggtcaaagct aaaattggaa catgattttg tttcaatatt tctgcggtac gaaggatcac
    50221 attgttttca cccgtataag aaatcagaat cccgcacata tcactcgtgc aattgtacgc
    50281 ttcatagaat gattcaccaa aagtggtgac aatcgtgaca tccattttga tgcgcttcat
    50341 tttcagcatg aaatcttgag aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt
    50401 ttttgcttga agcattagtt gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag
    50461 tgtatcatga atcgtcatct gttctaaaga agccatttta tttgcgatcg tcataatacc
    50521 gtctgtggtg gaaaaaggaa gattcgcatc tacgtcttga aaattccctt caagataggc
    50581 tgtttcagct aaaaaagcat tacgaaaact gacccagcca tcaaaaccca ttttttttgc
    50641 tacacggatg agtgtagagg ggtggacata acatcgctca gcaatcgctt ggatggtcat
    50701 ttcgccaagt gctggagaat cgcttaagag aaactggatc acttcatttt ctgaaggaga
    50761 aaaggagatt tctttgattt tttcacgtaa taacatgtag ataccttctt cttatttttt
    50821 tacgtaaatt ataacttttt tgaaaatgat ttgtaaacgg atttatacta tataacttaa
    50881 aatagacatt ttttgacaag atatttacaa aaatccacta gaaaacagta aattttgtaa
    50941 atgttactgg aaagaaacag atttctattg tttgttttgt ttacaaaaaa gataatgaaa
    51001 gtgtaaaaaa gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg
    51061 gaggcagcag ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac
    51121 cgatacgaga aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtta
    51181 gtcagatgtc tcagcgtatg tgggatgctt ctccgtatga agtcaagatc tatgcaactc
    51241 tggatcggga agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc
    51301 aattagatgc acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag
    51361 aaacaaatgg tgctggcggg atattcaaag cttttcgtac cgttccagtc gttttggcaa
    51421 tcgttgagga catgaagcgt ttatgtccaa atgcttggct gatcaatttc gcgaacccaa
    51481 gtgggatggt gacagaagca gttatcagat atggcaaatg ggataaagtg attggattat
    51541 gcaatgtgcc agtgatggcg atgatgacag aaccagcaat gcttggcaag aaaaaagaag
    51601 agctgatcta caagtttgcc ggtctaaatc atttccattg gcacaaagta gcagataata
    51661 aaggaaatga ccgcacggca gaattgattg atcgtttata cgatgaagag aatggattac
    51721 cgaaaaatat ctttgatatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc
    51781 cttgtggcta ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag
    51841 aatatcaaac gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctatttg
    51901 aattatacaa agatccagaa ttagaccata aacctgaaca attgcaaaaa cgaggtggcg
    51961 cttattattc agacgctgcc tgtgaaacga ttgcttcaat ctatggaaat aaagaaacac
    52021 agatcgtggt atctacaaag aatgatggag ctgttcctga cctgccggca gattgtgtag
    52081 tagaagtcac tgcctatgtt ggcgcacaag gagcgagaaa tgtagcgttt ggttctcttc
    52141 ctgctgctga aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag
    52201 cagctgtgac tggtgattat gggacagctt tacaggcgtt tacgatcaat ccacttatac
    52261 gatcaggaaa gacagcaaaa cggatcatgg atgagttgtt tattgcacac cagcagtatt
    52321 taccgcaatt caaggagaca atcagccgat taaaagaaga aggaatcgaa gttcaagatg
    52381 aactggcaag agaattatca ggtaatacac atataatgga ggaagtataa tgggattcag
    52441 aaaagatttt ttatggggcg gtgctacagc agccaatcaa tgtgagggcg gttatgatga
    52501 aggtggacgc ggactagcaa acgtagatgt tgtgccaatc ggaaaagacc gttttcctgt
    52561 tatcaccgga aagaaaaaaa tgtttgaatt tgatgaggat catttttatc cagcaaaaat
    52621 tgcaatcgat atgtatcatc gttataaaga agatattgct ttgtttgcag aaatggggtt
    52681 caagacttat cgaatgtcca tcgcctggag tcgtattttt ccaatggggg atgagaaaga
    52741 gccgaatgaa gctggcttga aattttatga ggatgccttt aaagaatgcc aaaagcacgg
    52801 aattgagcca cttgtgacga ttacgcattt tgattgtcca atgcatttga tcaaagaata
    52861 tggtgcgtgg cgttccagag aactcgttgg tttttatgaa aatctgtgtc gagtcatctt
    52921 caatcgctat aaaggacttg tcaaatattg gctgacattc aatgagatca atatgatcct
    52981 tcatgcacca ttcatgggcg cagggctgta ttttgaaaaa ggagaaaatg aagaacaaat
    53041 caaatatcaa gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agattgctca
    53101 tgaagtagat ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata
    53161 cacttgcaat cctgaagatg tttgggcttc tagaaaatca gatcgagaaa attatttctt
    53221 cattgatgtc cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggagcgaaa
    53281 tggcattcag atcgaaatgg aaccagggga tgccgcattg ctgaaagaac atactgttga
    53341 ttttatctct ttttcttatt atgcttcccg agtttcgact accgacaacg aactcttgga
    53401 acagactgca ggaaatatct ttgcttctgt caaaaatcca tatctgcaag caagtgaatg
    53461 ggggtggcaa attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta
    53521 tcaaaaacca ttgtttattg tagaaaacgg cttaggtgca gtcgatacgc cagatgaaaa
    53581 tggttatgtg gaagatgatt accgaatcga ttacctggca gcgcatatcc aggcgatgaa
    53641 ggatgcagta gaactagacg gagtcgatct attaggctat acgacttggg gatgtattga
    53701 tttagtatca gctggtaccg gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg
    53761 tgataaccaa ggaaatggca cattagcccg ttcaaagaaa aaatcattca actggtataa
    53821 aaaagtaatt gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag
    53881 tctattgttt ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga
    53941 acaatagact ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa
    54001 tctacatatg tatgatgtaa tcgttattgg cgcaggtcct gctgggatga ctgctgcttt
    54061 atatgcttca cgttcaaact tatccgtatt gatgatcgaa caaggtgccc ctggcggcca
    54121 aatgaacaat acggcagaaa tcgaaaatta tcctggattc gattcgatca tgggccccga
    54181 tctagcttat aagatgtatg aaagtgtctc aagatttggg acagaaaatg cttatggaat
    54241 cgttcaagat atcaaggatc acggcagcca taaagaagtc atctgtgaag ataaaagtta
    54301 cgaaggaaaa acagtcatta tcgcaacagg atgtatccac cgtaaattag gcgtacctgg
    54361 cgaggaagaa tttgctggac gaggcgtttc ttactgtgca gtctgtgatg gtgcgttttt
    54421 ccgtaacaaa cgtctagtag tcatcggggg tggggactca gcagtagaag aagccatcta
    54481 tttgacacaa tttgcttctg aagtagtgat cgtccaccgt agagatgaat tacgtgcaca
    54541 aaagatcatc caagaccgtg catttgcaaa tgaaaaaatt tcctttatgt gggatacagt
    54601 cgttgacgaa atcgttggaa acgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa
    54661 aaccggcaat gaatttgatt tagcagcaga cggagtattc atctatgttg gactggatcc
    54721 attaactgca ccttttagag aagcaggttt gacgaacgaa gctggctgga ttccaacaga
    54781 caacgaaatg aaaacgaata tgccaggcgt ttttgccatc ggagatgttc gtgaaaaaga
    54841 tttgcgacaa atcactacag ctgttggtga cggcggtatt gctggacaac aagtgtataa
    54901 atacttggag tctttagaag aaacaacagc ataagtttaa aaagagtaga agaacgggtt
    54961 caattgttct tttactcttt tttttcgcgt gaaatttact gaatctgatt tatgcgaaca
    55021 cgtccaacac cttttgataa aagaatcctt tcttgattta agcgtttttt ggtcctcata
    55081 ctttcatatc taataagtga atgctatact tattaagaac catatttcag aaagaggtgt
    55141 tattttcatg tcttgggaac aagtgtatga acaatgggta catgaagaaa atttagaaga
    55201 aaacttaaaa aaacaattaa atgatctggg tgaagatcct gaaaaacttg aagatgcttt
    55261 ttatgcaccg ttggaattcg gtactgcagg tatgcgggga atcttaggac ctgggatcaa
    55321 tcgaatgaac atctatacgg taagacaagc aacagaagga ctagcacgtt tcatggatac
    55381 acaaggtgaa gaaacaaaac gccgaggtgt cgctatcgct tatgactcac gccatcagtc
    55441 accagaattt gcaatggaag cagccaaaac actagcacat cacaatatcc cgtcttatgt
    55501 attcgaaagt ctgcgtccaa caccagagct ttcttttgca gttcgtcatt tgaagacgtt
    55561 tgcgggaatc atggtaaccg catctcacaa cccagcggct tacaatggat ataaagtcta
    55621 cggagaagat ggcggacaaa tgcctcctgc agacgcagat gcattgacga aatatgtccg
    55681 tgaagtcagc aatccgctaa aagtcgaagt attatctgac gaagaagcta aacatagcgg
    55741 gttgatcacg atcatcggag aagaagtgga cgctgcttat ctagaagaaa tcaaagcagt
    55801 gacgattgat cgtgaattgg ttgaaacaat ggggaaagat ttgaaacttg tgtacacgcc
    55861 acttcatgga acagggaaaa tgttgggcga acgtgcgttg aaacaagctg gattcgaaca
    55921 attcgtacta gttcccgaac aagctgtggc agatccagat ttcactacgg tcaaatcgcc
    55981 aaatccagaa gaacattcag cttttgaata tgctatacga cttggagaaa aagaaggagc
    56041 tgacttgctg attgcaacag atcctgatgc agaccgttta ggcgcagcgg ttcgtttgcc
    56101 agatggttct tatgaagtat tgactgggaa ccaaatcgga gcattgtttg ctcaatatat
    56161 tttggaagca catcaatcaa gaggcacttt accagaaaac gcagcggtat tgaaatcaat
    56221 cgtttccagc gaattgccta cagcaattgc ggaacactac ggtgctacca tgttcaatgt
    56281 gttgacaggg tttaaattca ttgctgaaaa aatccaacaa tttgaagaag accattcaca
    56341 tacttttatg tttggattcg aagaaagtta tggttattta gttaaacctt ttgttcgcga
    56401 taaagacgcg atccaagcat tgcttttatt agcagaagta gcagcttact ataaaaaaca
    56461 aggcaaaacg ttgtatgacg gcttgcaaac gatttttgaa acctatggct atttcgcaga
    56521 aaaaacgatt tctgtgacaa tgagcggaca agaaggttct gcgaaaatct ctgctttgat
    56581 ggaaaaattc cgtaaagaag caccgacaga atttgccgga gcaaaagtta ttcaaacaga
    56641 agactttaaa gaattgacga gaacagaccg tgaaggaaac gtagaaaaac taacaacacc
    56701 gccatccgac gtattgaagt atacattgga agaaaatggc tggattgcta tccgcccttc
    56761 aggaactgag ccgaaaatca agttttatat tggtgtaaaa ggaagttcta cagaagacgc
    56821 agccgaaaaa gtagcctctt atgaagcgat tatcaaaaag atgacagaag aataaaaaac
    56881 ttgagactga tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg
    56941 aatggattgc cagacacgca aaaatcaaaa gtagagaaag tcagtcaatt attcaaaatg
    57001 ttgagtgatc ccactcgtct taaaattctg ctatatctga aagacgggga acaaaatgtg
    57061 acagcaatca cccaagcagt ggaaatggag cagtctgctg tatcgcatca actacgttta
    57121 ttaagagaaa atcatgtcgt caagtctcat cgcgagggaa aagcgatttt gtatagtctt
    57181 gatgattctc atgtactgga tattttgaac cagacactca aacatgtgga acatcaataa
    57241 cgataggtgc aaatagataa acactggagt gtgacaacga agaaatttcg ttgtcacact
    57301 ccagtgtttt ttaagaaaaa gacaaaaatg atcaatcgct tttttctgtt gtttcagttc
    57361 cataataact atgatcttct aggtctaaag cattcaaaat aagcgaagcc gtttcttcaa
    57421 tagataaaga tgctacattg atcactacac atcctagttt ttcataaaga tcattcgcaa
    57481 aatcgagttc agctctaatt ttttcgatat cggaataagc agtatcaggg tttaatccgt
    57541 aagagcgcat ccgttcttta cggatattat ttaaaatatc tgggttattt gttagtccca
    57601 cgattttttt aggatcgatc tcccaaagct gtttgggaat atgtgcttgt gggatcaaag
    57661 gtaaattggc aacttttaaa tttttgttag ctaaaaacaa gctcaaaggc gttttggaag
    57721 ttcgggatac gcctagcaaa acgacatctg cttctaaaaa accacgagga tctttcccat
    57781 catcgtattt tacagcaaat tccattgctt tgatccgttt gaaatagttc tcattcaata
    57841 aatggagggc accaggttgc cgaattggct tgataccagt taaacgttca atttctgata
    57901 tcggtggcgt catcacatca aaactaaata attgatgttc ttcacaaaaa gaacgtgtta
    57961 tctcgatcag ttcttgattg attaatgtat gcaggacaag tgctttttct tctaatgcat
    58021 cttgcaatgc ctgccttaat gttttttcat ctgtcacgaa cgttctgcgg aataggttga
    58081 actcaacggt aggatactga gccatagtag cttgcgctag ttttgtcgca gtttctccag
    58141 cagagtcgga gatgacaaaa aaagtaacga tttctgtaga atcggaagtc atggataaaa
    58201 cctcacttgc atttttttta tgtttattat accatactct agaatgcgta cttcgtaatc
    58261 attttgattt ggagggataa gtcatgaaaa aacagacatt gatcgaacaa tcattagaaa
    58321 cattgaaaaa taacggtttt aaatatacta aaaaaagaga agcattgtta acttatttag
    58381 tgaagcgtaa ccgctacgta tctgctaaag aagtttttga ctatatgaat caggaattca
    58441 aaggggtaag ttatgatacg atttaccgga atctccatga ttttgcagag atcgatttac
    58501 tggaagaaac cgatttaaat ggagaaatga agtttcgttt tcattgctgt caggggaaaa
    58561 tcggccatca ccatcatttt atctgtacag tgtgcggaac aacgaaagaa atccatatgt
    58621 gtccgatgaa ttatttcgaa gaacagctag gtggatgcac gatcgaaggg catagattcg
    58681 agattttcgg aagatgtgaa aattgtcaat aaggatttaa ataaaataac atttgataat
    58741 tttaaaaaaa agcaagattt ccttgacggt caaaacagca ttagatataa tatttacagg
    58801 acagtcttgt tttttgagat atatttcaat aacaattacg gttacttttt aagctcggag
    58861 ggagggaatt aacatgtcaa aaacagtcgt tcgcaaaaac gaatcacttg acgatgctct
    58921 tcgtcgcttc aaacgttccg tgtcaaaagc gggtacttta caagaatctc gtaaacgtga
    58981 attctacgaa aaaccaagtg taagacgcaa gaaaaaatct gaagcagcta gaaaacgcaa
    59041 aaaattctag ttctgtgatg aattggagtt ggtagtgtgt cactactaag tagattaaac
    59101 gatgacatga aagcagcgat gaaagcgaaa gataaggaat ctttacaagt catccgaatg
    59161 atcaagtcat ctatacaaaa cgagcagatt aaagaaggtc acgacttgac agaagaagaa
    59221 gaactaacgg tgttgtctcg tgaaatgaaa cagcgacgtg attctttgca tgagtttgaa
    59281 gaagcaggac gtgacgattt agctgaaaaa gtaaaaagtg aaattgtaat agtcgaaaaa
    59341 tatatgccgg aacaactttc cgacgaagaa attcgacaac ttgttcaaga agcaatcact
    59401 caaactggtg cttcatcaat gaaagaattt ggcaaagtta tgggtgcaat tatgcctaaa
    59461 gtcaaaggaa aagcagatgg aaaccaagtg aatgcgattg tgaaagaact attacaatct
    59521 taattttatc atttaaactg cggacagtga ttatccgcag ttttttgtgt tcgtaaaaaa
    59581 agaactaaaa aacagctaga agaaagaatc ttatt
//
LOCUS       DAEGIQ010000003        59444 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000003 DAEGIQ010000000
VERSION     DAEGIQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.003"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(122..1171)
                     /gene="recA"
                     /locus_tag="KMT45_000151"
     CDS             complement(122..1171)
                     /gene="recA"
                     /locus_tag="KMT45_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBE7003643.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1280..2521)
                     /locus_tag="KMT45_000152"
     CDS             complement(1280..2521)
                     /locus_tag="KMT45_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBE7003644.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2761..3339)
                     /gene="pgsA"
                     /locus_tag="KMT45_000153"
     CDS             complement(2761..3339)
                     /gene="pgsA"
                     /locus_tag="KMT45_000153"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBE7003645.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3548..4471)
                     /locus_tag="KMT45_000154"
     CDS             complement(3548..4471)
                     /locus_tag="KMT45_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7003646.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5210..6496)
                     /locus_tag="KMT45_000155"
     CDS             complement(5210..6496)
                     /locus_tag="KMT45_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBE7003647.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6489..7757)
                     /locus_tag="KMT45_000156"
     CDS             complement(6489..7757)
                     /locus_tag="KMT45_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBE7003648.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8009..8470
                     /gene="mscL"
                     /locus_tag="KMT45_000157"
     CDS             8009..8470
                     /gene="mscL"
                     /locus_tag="KMT45_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBE7003649.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8529..8975)
                     /locus_tag="KMT45_000158"
     CDS             complement(8529..8975)
                     /locus_tag="KMT45_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7003650.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9068..9313)
                     /locus_tag="KMT45_000159"
     CDS             complement(9068..9313)
                     /locus_tag="KMT45_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBE7003651.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9428..10006)
                     /locus_tag="KMT45_000160"
     CDS             complement(9428..10006)
                     /locus_tag="KMT45_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBE7003652.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10083..10613)
                     /locus_tag="KMT45_000161"
     CDS             complement(10083..10613)
                     /locus_tag="KMT45_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBE7003653.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10617..11477)
                     /gene="rlmB"
                     /locus_tag="KMT45_000162"
     CDS             complement(10617..11477)
                     /gene="rlmB"
                     /locus_tag="KMT45_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBE7003654.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11470..11865)
                     /locus_tag="KMT45_000163"
     CDS             complement(11470..11865)
                     /locus_tag="KMT45_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBE7003655.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11862..13274)
                     /locus_tag="KMT45_000164"
     CDS             complement(11862..13274)
                     /locus_tag="KMT45_000164"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBE7003656.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13271..13807)
                     /gene="cysE"
                     /locus_tag="KMT45_000165"
     CDS             complement(13271..13807)
                     /gene="cysE"
                     /locus_tag="KMT45_000165"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBE7003657.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13893..14132)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14129..15589)
                     /locus_tag="KMT45_000166"
     CDS             complement(14129..15589)
                     /locus_tag="KMT45_000166"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBE7003658.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15759..16916)
                     /locus_tag="KMT45_000167"
     CDS             complement(15759..16916)
                     /locus_tag="KMT45_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBE7003659.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16950..18320)
                     /gene="radA"
                     /locus_tag="KMT45_000168"
     CDS             complement(16950..18320)
                     /gene="radA"
                     /locus_tag="KMT45_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBE7003660.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18383..18910)
                     /locus_tag="KMT45_000169"
     CDS             complement(18383..18910)
                     /locus_tag="KMT45_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBE7003661.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19140..19946
                     /gene="proB"
                     /locus_tag="KMT45_000170"
     CDS             19140..19946
                     /gene="proB"
                     /locus_tag="KMT45_000170"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBE7003662.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19943..21190
                     /locus_tag="KMT45_000171"
     CDS             19943..21190
                     /locus_tag="KMT45_000171"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBE7003663.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21266..21610
                     /locus_tag="KMT45_000172"
     CDS             21266..21610
                     /locus_tag="KMT45_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003664.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21727..22053)
                     /locus_tag="KMT45_000173"
     CDS             complement(21727..22053)
                     /locus_tag="KMT45_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003665.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22668..23870)
                     /locus_tag="KMT45_000174"
     CDS             complement(22668..23870)
                     /locus_tag="KMT45_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7003666.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            23987..24502
                     /locus_tag="KMT45_000175"
     CDS             23987..24502
                     /locus_tag="KMT45_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBE7003667.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24701..25147)
                     /locus_tag="KMT45_000176"
     CDS             complement(24701..25147)
                     /locus_tag="KMT45_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBE7003668.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(25194..26672)
                     /gene="msr(C)"
                     /locus_tag="KMT45_000177"
     CDS             complement(25194..26672)
                     /gene="msr(C)"
                     /locus_tag="KMT45_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBE7003669.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(27026..27655)
                     /locus_tag="KMT45_000178"
     CDS             complement(27026..27655)
                     /locus_tag="KMT45_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBE7003670.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(27639..28103)
                     /locus_tag="KMT45_000179"
     CDS             complement(27639..28103)
                     /locus_tag="KMT45_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE7003671.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(28590..29858)
                     /locus_tag="KMT45_000180"
     CDS             complement(28590..29858)
                     /locus_tag="KMT45_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBE7003672.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(30089..30826)
                     /locus_tag="KMT45_000181"
     CDS             complement(30089..30826)
                     /locus_tag="KMT45_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE7003673.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(30893..31462)
                     /locus_tag="KMT45_000182"
     CDS             complement(30893..31462)
                     /locus_tag="KMT45_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBE7003674.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            31590..32462
                     /locus_tag="KMT45_000183"
     CDS             31590..32462
                     /locus_tag="KMT45_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE7003675.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            32804..33676
                     /locus_tag="KMT45_000184"
     CDS             32804..33676
                     /locus_tag="KMT45_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE7003676.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            33673..35631
                     /locus_tag="KMT45_000185"
     CDS             33673..35631
                     /locus_tag="KMT45_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBE7003677.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(35963..36814)
                     /locus_tag="KMT45_000186"
     CDS             complement(35963..36814)
                     /locus_tag="KMT45_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE7003678.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            36937..37404
                     /locus_tag="KMT45_000187"
     CDS             36937..37404
                     /locus_tag="KMT45_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBE7003679.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(37667..38149)
                     /locus_tag="KMT45_000188"
     CDS             complement(37667..38149)
                     /locus_tag="KMT45_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBE7003680.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(38249..39112)
                     /locus_tag="KMT45_000189"
     CDS             complement(38249..39112)
                     /locus_tag="KMT45_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBE7003681.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            39710..40585
                     /locus_tag="KMT45_000190"
     CDS             39710..40585
                     /locus_tag="KMT45_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003682.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(40730..40882)
                     /locus_tag="KMT45_000191"
                     /pseudo
     CDS             complement(40730..40882)
                     /locus_tag="KMT45_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(41150..42481)
                     /locus_tag="KMT45_000192"
     CDS             complement(41150..42481)
                     /locus_tag="KMT45_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7003683.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(42630..43343)
                     /locus_tag="KMT45_000193"
     CDS             complement(42630..43343)
                     /locus_tag="KMT45_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7003684.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            43518..44942
                     /locus_tag="KMT45_000194"
     CDS             43518..44942
                     /locus_tag="KMT45_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBE7003685.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            45241..45948
                     /locus_tag="KMT45_000195"
     CDS             45241..45948
                     /locus_tag="KMT45_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBE7003686.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            45935..46258
                     /locus_tag="KMT45_000196"
     CDS             45935..46258
                     /locus_tag="KMT45_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBE7003687.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(46347..47384)
                     /locus_tag="KMT45_000197"
     CDS             complement(46347..47384)
                     /locus_tag="KMT45_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBE7003688.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(47705..48688)
                     /locus_tag="KMT45_000198"
     CDS             complement(47705..48688)
                     /locus_tag="KMT45_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBE7003689.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(49103..49873)
                     /locus_tag="KMT45_000199"
     CDS             complement(49103..49873)
                     /locus_tag="KMT45_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7003690.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(49866..50801)
                     /locus_tag="KMT45_000200"
     CDS             complement(49866..50801)
                     /locus_tag="KMT45_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7003691.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(51117..52766)
                     /locus_tag="KMT45_000201"
     CDS             complement(51117..52766)
                     /locus_tag="KMT45_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBE7003692.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(52883..55486)
                     /locus_tag="KMT45_000202"
     CDS             complement(52883..55486)
                     /locus_tag="KMT45_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBE7003693.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(55498..56547)
                     /locus_tag="KMT45_000203"
     CDS             complement(55498..56547)
                     /locus_tag="KMT45_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBE7003694.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(56573..56887)
                     /locus_tag="KMT45_000204"
     CDS             complement(56573..56887)
                     /locus_tag="KMT45_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBE7003695.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(56903..57349)
                     /locus_tag="KMT45_000205"
     CDS             complement(56903..57349)
                     /locus_tag="KMT45_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE7003696.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(57350..59272)
                     /locus_tag="KMT45_000206"
     CDS             complement(57350..59272)
                     /locus_tag="KMT45_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBE7003697.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT        17297 a        12380 c        10350 g        19417 t
ORIGIN      
        1 ttttatttta tgtacccact cgtataatta aataactcac gatttcttta ttcaataaaa
       61 aaacggcagg tcaaggagag tacctactct tcagaccagc cgtttttgtt tatcataatt
      121 tttattcttc tactaaaggc aattcttctt gtcctttttc atcttctggg acagcgactt
      181 cttcaccaat accataagct gctctaacta gagcagaaac ttctgccatc atttcaggat
      241 gattagccat atagatttta gcgttttccc gtccttgacc aatccgatct tctttataag
      301 aataccaagc accgctttta tcaacaatgt ctttttctac tgccatgtct agaagttctc
      361 cttcttgtga gatccctaaa ccatacatga catctacttc agcgattttg aaaggaggtg
      421 ctactttgtt ttttacgact ttgatctttg tacggttacc aacaatatct gtaccttgtt
      481 tcaattgttc tgcacgacgt acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc
      541 ctccaggagt gatttctgga ttcccaaaca tcacgcctac tttttcacgg atttggttga
      601 tgaaaatagc gattgttttt gttttgttga tcgaaccaga gagtttacgc aatgcttgag
      661 acatcaaacg tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg atttctgctc
      721 gtggaactaa agcagcaact gaatcgacta ctacgatatc tacagcccca cttgatacta
      781 aagcatcagc gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat
      841 cgatattcac acctaatttt tgtgcatatt gcggatctaa cgcatgctca gcatcaatga
      901 aagcggccgt tccgccattt ttttgtactt ctgcaatagc gtgtagtgca actgttgttt
      961 tacctgaact ttcaggtcca tatacttcga tgatacgtcc acgggggtaa ccgccgacac
     1021 ctaaagcgac atctaacgca agagagccac ttggaattgt agagatttgt tggtcgattt
     1081 tttcgcccaa tttcataata gagcctttac catagctctt ttcaattttt ttcagtgcag
     1141 catctaaggc tgctttacga tcatctgcca atacgataat cctccttata tttacaaatc
     1201 agagcaacat ttcattatcc ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc
     1261 gaacaaatat tcgcatttat cattttctta aaagctgttt tctcaaaaga tcaaatcctt
     1321 gcataaccgc attatatctg atagcagcac gatctctggc aaagtgatat ttttccgaaa
     1381 ccacgccgtc tttagatgaa atagcgatcc atactgttcc tgcagattga ccttctaacg
     1441 tgtccggtcc agccactccg gtgaaagcta cactgtaatc tgttcttgct atctcacgtg
     1501 cttgaatggc catctgctcc acacattctt tgctgactgt tccatattct gctaatagct
     1561 ccggatcaat ctccagaaaa ccagcttttg tttggagcga gtaggtaacg aatccgcctg
     1621 gaaatacttc agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca
     1681 aactttccgc tgctgttaca gtctgcttgt tttctttcag tagttccact acgacttttg
     1741 ctagcgaatt atcatcgccg tatccataaa aatattcccc tactcgttct tggatttttt
     1801 cttctaatgc taaaagagct tgattccctg cctgaacatc atttgttttc actgtcagtc
     1861 gtaatgtcac ttcatttggt ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa
     1921 tcagatcttt tagctcagtg accagtcgcg attcaccaat cccataaaag cgcaaaactt
     1981 tagaaattag tttttcttca gaaggaaaac gttcctctag taaaggacgt acttgttcca
     2041 caaacattgg tttcaactca ttaggcggac cgggaagaag gatatacgca tgtgtatctg
     2101 tttgataaaa agtccctaaa gcaagccctg tacgatttgg tagaggaacc ccgccttcga
     2161 taatctggga ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg
     2221 ccaataattt tttgtatcct tccgtatttt gtatgagaga cttaccgagg tgagccgcag
     2281 ttgcttcttt tgtcaggtca tcttctgtcg gtcctaatcc gccacataag agtatgagtt
     2341 cacttcttgt ttcagctaga gcaatcaact cttctaaacg ctgctgatta tctcctacaa
     2401 ctgtttgata ataaacttcg atacctagat cagctagctg ttctgacaag aaagtggcat
     2461 tcgtattcac gacttgtcct agtaaaagtt ctgttccgac agctattatt tctgctttca
     2521 ttacttcccg ctcccttata tagtatacgc tgtattttta tattttttta tttaaaaaga
     2581 gggcaaggag ctgtgacaaa aatcttgtca cagctccttg ttccaaataa acagtgttct
     2641 cctgccaaaa cctcggtcat aagtcacaat atacataaaa catgaaaagc tgttttttga
     2701 aattatccct tgtgactcag agttagacgg caggctcaca acctctccac taaacgatac
     2761 ttacattgat cctttgaaca caccgctgtt tttgatgaag taatccacac ctgaatagat
     2821 agtaaagatc agacaagcat aaagcatgat ttgatctact ggaataccga tcaggctgaa
     2881 tgggatatta ttgataaaca gcaagataat agcaaccatt tgtgtagccg tcttgacttt
     2941 tcccggccaa gctgcagcca tgacttctcc atgttcgaca agtagtaaac gcaagcctgt
     3001 gacggctaat tcacgacaga caatgatcgc tactacccaa ccaggggcct ttccttggtc
     3061 aaccaacatg ataaaagcag tcataactag cattttatct gccaacgggt ccgcaaactt
     3121 gccaaaatta gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat
     3181 actagctaaa gcaaaaataa ctgcacctac tagctgtgtg acagccagtg tggtgtctcc
     3241 tacgttaaat tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat
     3301 acaaattcta agtacagtta atttatttgg taaattcaca ctatcgactc ctttcttccc
     3361 tattctacgt gactatcagc gaagtttcca ccgatattat ttacttttcc aaatgaattt
     3421 attcaaagga gtatttttat tgaaattgat ctttttgaat taacgaagaa ggaccgcgac
     3481 acagatttgt cacgatcctt cttttttata cctacaagta aacagatgcg taaactaatt
     3541 tatacgatca ttctgcgtat tgaagagtaa ggttcacatt ttttggactc tgattgtttt
     3601 cccctggatt aactggtacg gattctccat tcgctttgat cgttgcattg cttgctgcac
     3661 caatcgtgat ggtcgcattt gttgctgttt ccggtaacac ggtactctgt gtttcatttg
     3721 ctgccagtgt cccttgataa acataggcat tgtctactag cacacctaac catacacgat
     3781 tgacagcagt aaattcaagg gttacaggct tggtagcgtc tgtgatattt actgcgatgg
     3841 cactgccaga atcttctcct gtagctattg ccattttctt ttcttcactt gattttgttg
     3901 ttgattctac tgtagaagac tctttggtct gcgtgctttg ctctgaggaa gaggactcct
     3961 cgactgatcc ttctacttta agcgaagaag ctgtgtctcc gattattgga tcagcttgac
     4021 gatcttgcca agtcatatag cccacgacaa cgacaatcgc caaagcaacc aatcctaata
     4081 aaatgacagg aagacttgtc caaaagcgac ttggatgcga ttcttcctga tgcattgctt
     4141 ttctagaagt acttactgtt tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc
     4201 ttttcccatc aaaaacatcg actaaatggt ctccatcttc tcctacagct tcagcgtatt
     4261 gccgaataaa tgcccgcacg taaaatttac caggcaattg gtcaaaatca tcattgtcga
     4321 ttgcttccaa ataacgtttt tgaatttttg taatctgctg taattcatct aaagatattt
     4381 ttttattcag gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc actcgttcat
     4441 cttctccagt ctgtttgatt ctccgaagca tctgctgttg caacgaaaga cgttatgttt
     4501 gggtgctgtg acaaaagtca tgtcacagct ccttttttcc gaataaacat tgttctcctg
     4561 ccaactcctc ggtcacaagt cacaatatcc aataaatata agaagctgtt ttttgaactt
     4621 gttccttgtg actcggagtt aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa
     4681 tcagctgttc ctcggtatta gctcaaaaac cggcaaaata taaggagata tttttccgta
     4741 tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttcctta aacggtgttc
     4801 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacattaaga agctgttttt
     4861 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt ggtacacggt
     4921 gttccagaag tagcttcttc ggcaataagc cgaaatatca caaaaatttg agaagcaatt
     4981 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct ctttgattac
     5041 ttcgtttgat tttattttaa tataagttga ttaaaagaga gcatgatttg ttttattttc
     5101 tcactaattt ttccaacaca actaagaata ccataaaagc tgattttgga ggaggttcct
     5161 actacaagtc taaaacgaaa tttaagactt ttcctcataa aatactggtt tattttggtc
     5221 tcatgtaaaa ctcgctgaag gcctcttcac ttataaatgc ttccccagct gccagtacgt
     5281 ctttcatttg gatcgaatcg atgatttcag gaagatcaaa taaagttcga tcgccaaata
     5341 aactttgcgt aaattgattg gcaatatatt cgatcgaatt taatgattgg aaatattgtc
     5401 caagcatttt cttcttcagc aaatcgagat ttgtttctga cacttccgga tcatcagcga
     5461 atccaagaat gatttctttg actttttctg ctgctttctc tggttcgtcc gtatctccgc
     5521 tgaagtccgc aaagtgaaat tcacgatcca agctgaattc aaaaccaaag ctgtcatcga
     5581 taattccttg attgtacatc gctagatagt ttcgagaagt attgcctaaa atcatttgga
     5641 acaacagatt gattgctgtt ttatagcgta acagttctgt tccttcttgc ggcaatgtgt
     5701 ctaatccttt aataccaagt acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt
     5761 gtttgatgat ttcttttgtg ttttcaggga aataacgtac aatttcttgc tttggaggga
     5821 aattttttgc ttcttggttt tcccgaataa gtttcatcaa tttctcaggc tccatcttac
     5881 caactacaaa taaaaccata ttgcttggct gataaaacgt ccgatagcac gtatacaagt
     5941 cttgagctgt gattttgtcg atactttcaa ccgtgcccgc aatatcgata tgcaatgggt
     6001 gtgtaggata caagttattt agtatgccaa aaaacatccg ccagttaggg tcgtcttcat
     6061 acatttggat ttcctgaccg atgattcctt tttccttgtt tacagtttct tctgtgaagt
     6121 aaggtgcttg tacaaaatcg atcagagtcg tcagattctt ttctacctga tccgtcgtag
     6181 aaaacagata gctggttttc gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc
     6241 caaataattg aaagacatca ccatcttctt tttcaaacaa cttatgttct aaaaaatggg
     6301 caattccatc tggaactttc accttttctt tttcaccata gggaataaat tcgttatcga
     6361 tcgaaccata attcgtactg aacaaaccat atgtcttgtg atagtcattt ttaggcaaca
     6421 aatacacggt caaaccgttt ggcagtactt catgataaag tgtttcattg atttgctcat
     6481 attctgtttt attcatttgc atttcctcct gctaaaaaga agattgcctg caattcgatt
     6541 tgctcagcta cttgttgtat ctctttgatc gttacgctct ccatacgttt caaccattct
     6601 tcatcagata attttgtttc cggcagccag ctgtccaaaa aagaagcttc gattgccgct
     6661 tgcggagagt ctagagaaag cagatattgg tttctcagca ttgcttttgt ctgagacagt
     6721 tccaactctg ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt
     6781 acttgatttc ggttcttttc atcgataccg gtttggacac tcatgaatcc tcggaacgta
     6841 tccacgctac tagaggcata ataagctaga ctttctttct ctctgacatt catgaaaagt
     6901 ttagagtgag gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa acgttctggt
     6961 tcatcgtaat acacatttgt ctgataagct aggttcagct ttgcttgaac gattggctca
     7021 cgtacttgtc gttccttcac gatattcact tgaggttggg tataaaacag atctggataa
     7081 actgtttccc gcgactcaaa tggtaattgc ccaactgctt cagctgcttt gtccggatca
     7141 acatcgccga caacaaaaat gtcgatttga tcatttgcca tcatttcttg atagtaggac
     7201 gctagcgaac tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt
     7261 tgttccgggc tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa agtttgttta
     7321 tcttccttca aactttccaa atatagacgc agattgttct tttccaagtc aaacgtcaac
     7381 tgatcaaact gatgattttt aatatttgga taaaataaaa cctcttttaa aaaatctacc
     7441 gcttgagaaa aaaggtttgg atcattgaca tattcaccat tcacaaaact gatccctgcg
     7501 tttagccaat gtaaatttcc tttttttcta actgataatc caaagcttgc gccataaagc
     7561 tctgcaagtt gcgcacttaa ctttgtctga tccgaataat tcaaactatt tgtttccaac
     7621 atgctgctga gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg
     7681 ataaaaatcc ggatcgtttt gtattgtttt gttggtaaga catgaagatt cacgccttct
     7741 gccaattgat acaccatatt tatatgctcc ttttcacagt tgattgcccg cttactcttt
     7801 taatgggtca acctgccttt tttacttatt cctctgcttg aaatttataa gttcaggcaa
     7861 ttcgccgttt tttctaaatg atgttacact ttatagtata gcatagatgt tttttatcaa
     7921 aagaaacaga cggctaaaag cgtatattgt ttttttattt tttatttttc gatatactaa
     7981 agacaaataa aagaagggag ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg
     8041 tggagatgtc ttggatcttg ctgtcggcgt tgttattggt agtgcgttta caagcatcgt
     8101 aaaccaagtg gtgcaaggct tgatcacacc actaataggt tggtttgtcg caatgatcac
     8161 aggtacttct gatttagaag gagcactttc aattcttgac tggtcgccga caaaaggcgt
     8221 cacatttgcg tttggtaacg ttattagtgc tatcattaca tttctgatca ctggttttgt
     8281 cttattctta gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca aagaagagca
     8341 agtggaagtg gaagaaactc ctactgcaga agattatctt agtgacatac gcgatcttct
     8401 tgccaaacaa gtggcagaag acaatacaca tccagtagag caaacaaatc tttccgatac
     8461 ttctaaataa taaagaatct aaaaaaaaga aagccgattt tcagtgtcgg ctttcttttt
     8521 ttagattctt aacttgctac ttgttccttt ttcatgtcga tatgtggaat atcatcttcc
     8581 aaatacacat ctgagattgc cttgaatcca aacgactcat aaaaagcttg taggtattct
     8641 tgtgcgccga tcacgattgg tttttctgga aaatttttct ggatcatgtt aatcgtttct
     8701 tgaacaatct ttcttcctaa accatcttta cggttgtcct gatgaacaag gacacgtcca
     8761 aaatggatac tctccgcttc ttcatatact cgcgtgtagg caaggatctt cccatcagta
     8821 tcttgaaaaa acgtatgcca agcttctcca tcaatgtcat caacttcttg ataaggacac
     8881 tgttgttcca caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa ctctgctgtt
     8941 gacaattctt caaatttctt tacctgatat tccactaagc aaacgccccc tgctttttta
     9001 ttcttagcat accgattcgc acacaaaaaa gcaaacgaac cttacgaatc tcaggctcgt
     9061 ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta agtacatctg
     9121 aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca acaacgaata
     9181 ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt cttcctgtct
     9241 gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg attgaagcta
     9301 aagttgttgg catacagttc acctctacta tcaactatac cacatatttt taaaattttc
     9361 aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc attaagattt
     9421 ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt tttactctcc
     9481 cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg tctacaaaat
     9541 caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac tggatttcgc
     9601 aattttcgat tgcttctttt aataaaagat gctgaattgc taaaggttct tgcaatgtga
     9661 tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag acttcatctt
     9721 tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt tgattgctta
     9781 taacgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa gttctcaatg
     9841 cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa tttcttaaat
     9901 aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg gcagcaaatg
     9961 cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa taattcctcc
    10021 ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat tagaacgata
    10081 tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt taactgctct
    10141 tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt ttcttgggct
    10201 attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt tcgtaatgct
    10261 ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt cacttgtgtc
    10321 aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt ttcgtcttct
    10381 ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc cggaactagt
    10441 tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt tgataaacga
    10501 tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg gacaagttcc
    10561 ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt catggtctac
    10621 tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac actggcattt
    10681 aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc ttttttcaat
    10741 cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc tttggcattc
    10801 catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc tgtttctttg
    10861 agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg ttcgactgcc
    10921 ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt tggtatgatc
    10981 acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt gtgtggatct
    11041 tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt tcgttcaatc
    11101 agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc ttgatgcacg
    11161 ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg cacagcttgt
    11221 tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc ttgaagaaag
    11281 agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc aaagacgaaa
    11341 ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc agtttgcttt
    11401 ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg atgtttgttt
    11461 gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga tcaattcttc
    11521 catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga accctgttgc
    11581 gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag catttcttcc
    11641 tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca tttgctgaat
    11701 caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca gcttattggg
    11761 ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa tcgcatctcc
    11821 aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc tcttctccat
    11881 cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa atcacgaatt
    11941 tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc gatcaattgt
    12001 tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat cgaaagccat
    12061 tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg ctggtctaga
    12121 taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc tgcattaaaa
    12181 tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc gattttatcc
    12241 gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt gtcaaacgta
    12301 ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact gtagcggatc
    12361 ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc aacgtttttg
    12421 atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc atcgtcttcg
    12481 ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc tgttttcgct
    12541 tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc gccaccatgg
    12601 atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat atgccaaccg
    12661 ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc ttgtttccat
    12721 aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg ctgacttgct
    12781 ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc aaatttacgg
    12841 gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac caacacttga
    12901 ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt agcaggttcg
    12961 acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc aacttctggt
    13021 gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc cgtgaaattc
    13081 gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt atcgaaagca
    13141 atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc gcagacatac
    13201 ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt caatgtattg
    13261 tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg acttttgctg
    13321 gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca cctgcaccga
    13381 ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca gagagcatgg
    13441 ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg cctaatgtta
    13501 cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg acgattccca
    13561 ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc tcaacaccag
    13621 ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg tgctgatata
    13681 aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc aaaacgatct
    13741 cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct cgggtaaacc
    13801 aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc ttcttataaa
    13861 gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc gtggttccac
    13921 ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt tcttggctac
    13981 tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc actcccagag
    14041 gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt gtaatgatca
    14101 gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa tgtgctaatg
    14161 atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc atttgtcctg
    14221 atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt tctttttgaa
    14281 cagctttgat ggcagctttg acagttggag catctactac ttccatttgt tctagctgtg
    14341 ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct tttgctgctt
    14401 cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt tctgcagcat
    14461 agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt gtcttttcat
    14521 ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg tcagtaaatg
    14581 catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt ttcgcatcga
    14641 aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat tcttggtgtt
    14701 cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag ttgaacattg
    14761 catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt gattcgtcac
    14821 gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt ccgaaggttg
    14881 gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg gcaatatggt
    14941 catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca acagcaaaat
    15001 tataagttgg cataccatcg cgtttttgga tgacaaagtc gccaccgata ttatcggatt
    15061 cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg cgaggcacac
    15121 ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct tcaggtgtca
    15181 agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt gcttcccgtt
    15241 ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta gcaagcagct
    15301 gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg ccgtattctc
    15361 caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt tccaactggc
    15421 ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga ataataaagt
    15481 ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca tttccaatat
    15541 gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt ttcttcctct
    15601 tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct tctcaaatat
    15661 catttattta aagccactca gcacaaattt taccttttct aaggtaaata gtaaagccaa
    15721 ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg tttagtcttt
    15781 ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt agcaaagatc
    15841 atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg ttcattcatg
    15901 taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc tactccttgt
    15961 tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg gatgacgact
    16021 ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg gaattcagag
    16081 actttattca agttatagtc attggttaca atgacaccat ctaaaagctt agccaatttg
    16141 atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat ttcgacagat
    16201 ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc acgcacacgt
    16261 ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa attagggatc
    16321 atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc gtcaatgatc
    16381 acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg ttctaacagt
    16441 tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga accaaaaact
    16501 ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc taagatgatc
    16561 atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta aagaggggtc
    16621 gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa gagaagataa
    16681 gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga gatatgtttg
    16741 gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt ggtaaatgta
    16801 ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg taaaaatgaa
    16861 atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt ttgcatcgtt
    16921 tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa cgtttcagcc
    16981 aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc taagttgttt
    17041 ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg ttgttcaata
    17101 tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca ttccgttggc
    17161 tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa atcaatcgct
    17221 ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt ctgaagaagc
    17281 aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa atcaagacct
    17341 gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg gatctctacc
    17401 aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc accgtctagg
    17461 cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg ctcatgcatt
    17521 tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg caaaatccga
    17581 aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg ttctaacatt
    17641 cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat ggctatccca
    17701 tttgttttag cggttttgag tagttctgca gtggtttctc ttacttggct gacacttcct
    17761 gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat aacataatca
    17821 ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc gtatagataa
    17881 aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc tgcgctttct
    17941 tcgccggata catataagac ttttccgcct atggctgcta attgctgaga aacttgtaac
    18001 agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga ccctggcacc
    18061 acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg aggttctttt
    18121 ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa acttgtccga
    18181 gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca ttttccgcaa
    18241 tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca aacaaattgt
    18301 acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag acattaaatc
    18361 aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct tgacgttgag
    18421 cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg ccgatacgct
    18481 cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac atgatatggc
    18541 cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg tttgtcaaca
    18601 ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat tcatcttctc
    18661 ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt tcattttctg
    18721 atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag cctgctttcc
    18781 agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca gcatgatgtg
    18841 tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact tcaaatcctc
    18901 tttttctcac cggttttcct ccctaaataa actactccta ttctaacata tccgaggctg
    18961 taaaagactt tctttacaaa ttcttcaaga aattctgcta tcttaaagta aaccattaat
    19021 tcgttctttt tccaaacgac tgcaagttgt acaaaaaaac tattttttac atgactttac
    19081 tcgtcaaaac aagtagaata gaacgaaatt aatgagtgga tatttgattg aggtgtcaca
    19141 tgcgtaaaga aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca agtacattga
    19201 tttatcccaa tggaaatatc aatcttgcgg caatcgatca gttagctttt gtcctttctg
    19261 atcttagaaa tcggggaaag gaagtgatct tagtctcatc aggagctgta ggcgttggta
    19321 tgcataaatt acatttgtca gaacgtccga ctttgattcc tgaacagcaa gctgcggcgg
    19381 ctgttggtca agccgaactg atgaatatct ataatcaaag attcagtgta tataatcaac
    19441 agacggcaca gatcctcgtg acaagagacg tggtagatta tcctgaaagt cgtaaaaatg
    19501 tgaggaatac attggaacag cttttgatta tggacacgat ccctatcgtg aacgaaaacg
    19561 atactgtagc agtggatgaa ttagatcatc tgacaaaatt tggcgataat gatcagctgt
    19621 cagccatcat ggcaaaactt gtcgcggcag accttttgat cgtcctttct gatatcgacg
    19681 ggttttattc agataatccg actacaaatc ccgaggctca gatgtatcgc tatatcgata
    19741 aaatcgatga aaagattctg gcacaagcag gtggtgctgg tagcacattt ggaacaggcg
    19801 gtatgcagag taaattaaaa gccgcttcta ggatatttga agcaaacagc gcaatgattt
    19861 tagcaaatgg aaaaaatccg gcaattattt ttgatattct tgcaggaaaa gaagtaggta
    19921 ctttatttaa ggaggacttg aaatgactga cttgatgcaa ttaggacaac aggcaaaaga
    19981 tagcgcgcaa caactagcac ttatgcctac aaaccgcaaa aatgagctac ttgttcaaat
    20041 ggcacaaacc atcaaaaaga atcaacaagc gatcattgaa gcaaatcata aagacttaga
    20101 aaaagcagtc gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc tgacacctga
    20161 gcgaatcaat gccatggcta cagagatcga aaaaattgct tcgttagacg atcctattgg
    20221 aaaagtagat gagatgtgga cgaacaccga cggattgaga atcggcaaaa agcgcgtgcc
    20281 actaggcgtg atcggcatca tttacgaatc tcgtccaaat gtcacgacag atgcagctag
    20341 tctagcattc aaatcaggga atgccgttat ccttcgcggt ggcaaagaag cattcttctc
    20401 gaatcaatta ttagtccaat tattgcagca agtactggtc agtgctggcg aatctcctta
    20461 tgccatccag tttgtcgatg atacttctca tgaaactgca ggacagttga tgcgattgac
    20521 agaatactta gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac gagtcaaaga
    20581 acaggcaacg gtacctatca ttgaaacagg tacaggaaat aatcacgtat atatcgataa
    20641 agaagcacag ttatctatgg cagtgaacat catcgtcaat gcaaaagctt cccgtccatc
    20701 tgtctgtaac gcggcagaaa cattgctgat ccattctgag atcgcacctt ttttcttgcc
    20761 agctattgaa aaagaactgg tagaacacgg ggtctcttta cgtgcggatg ctcgtgcgtt
    20821 agaatatcta gagacggctg ctttagcaga agacacagat tgggatactg aatatctaga
    20881 ttatatcctt gctgtcaaag tggtcgattc tttggatgaa gcaattgcac atatcaatcg
    20941 gcataatact aaacattcag aaacgatcgt gacagatagt tatgcggcta gtcaacgttt
    21001 cttaaatgaa gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt ttacagacgg
    21061 atcggtattt ggttttggtg cagagattgg tatctctact caaaagttgc atgcccgtgg
    21121 tccaatgggg ttaaatgaat taacttccac aaaatatatc atttatggcg atggacaaat
    21181 ccgcgaataa tgtttcgtgt gaaacattat tcccgccttt tgcctttttt ctgtaaaggt
    21241 tctatccaac tagaaaggtg tcaatatgtt tgaaatttta atgatttttt ttatttacct
    21301 tatttctctg aatatcgctg cttttctggg agtcggtatt ttatcccttt tctttcaatt
    21361 caaaaaacga agcataggga gtcaacggga aaaatggtcg cagtattttg acaaaatcgg
    21421 gcaaaaaggg ctggttacac gcttgcacat tagttacatg gttgcattat gcttattagc
    21481 gacaatcaat tactattctt ttttcgatca ttcaatcgct tatacgatta cgctactgat
    21541 tgctgggatt tttcacttat cttataaata tcagctgaat aaaaaccact tgaatcggac
    21601 tttccgctaa aaaatatccg tacgataagt agagtgcctt gaagatcggc aaaaaactgc
    21661 cggacttttt agaggctgtg acaaagattt tgtcacagcc tctttttcca tataaacggc
    21721 gttcgcctac cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg aaaatccgtc
    21781 aaatgtttcg ccatcataat aacgaagcag ctcatccatg gacaagttca tcgttttttc
    21841 tttttcttct tccgctgatt cagccagtag ctgttcccga tacacctgat acataccagc
    21901 tattttttgc tgcatcgctt ttctagtttc accttccgca ttgattattg gcaattcttt
    21961 gatatagcca cgataaatca accgatcatc tgaaaattca atgggtaatt caacgagtgt
    22021 caacccgact tcctgaaaat attcttccag catctattat ccccccattc tttttcttaa
    22081 atgaatttta gcttacctac tgaaaatttt ccagtttcat tattttgaga aaaaaaggga
    22141 atgtgacaaa aatcgtgtca catccccttt ttccgaataa atggtgttct tctgccaact
    22201 cctcaatcat aagtcacaat attcaacaaa catgagaaac tgttttttga aattgttcct
    22261 tgtgattcag agttaaacga cagtctcaca acctcttggt aaacggcgtt caatcagctg
    22321 accctcggta ttagctcaaa aaacggtaaa atatgagaag ctatttttcc gttttgtttc
    22381 tctaatactt aggataaaac gctgatttta caacctctgt taaacggtgt tcaatcagct
    22441 gtccttcggt attagctcaa aaaacggcaa aatatgagga actatttttc cgtttgtttc
    22501 tctaatactc aggacaaaac gctgatttta caacctcctg ataaacggtg ttcaaaagct
    22561 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct
    22621 gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta ttcttcttca
    22681 tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag aacaatggca
    22741 cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc tgcaataaat
    22801 ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc aacgtttcgt
    22861 tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat tccttggaaa
    22921 aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc agctcctaat
    22981 aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata agtcgtaaac
    23041 ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac accccagcca
    23101 aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg cgcatacgca
    23161 agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga acgaagttta
    23221 tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg tttctcagtt
    23281 tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc taccccaaaa
    23341 aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc aacagccatt
    23401 ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc aactaaaata
    23461 ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc ccagccggca
    23521 cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc gaatacaacg
    23581 atgataaaca atcctgtcat caatgttttt ttcaaaccga tccgactaga taagaaacct
    23641 gtaaatagca tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca
    23701 gctggcgtag cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact
    23761 cccataaaag cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt
    23821 tcttcatttg tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct
    23881 ctattagcat catacatgat ttattttaca tgtcaatatg cacatgtaaa acgtggaaac
    23941 tttactgtga tataataaag aaaataagac gagaggtagg atttttatga atactttagc
    24001 ctatatatta ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct
    24061 gatcacaatt ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga
    24121 ccagcagggt tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca
    24181 cttgactgat gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc
    24241 tacgcaaaaa gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac
    24301 agctactgga ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca
    24361 aaaaaaattg aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat
    24421 ccagcgcaga attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca
    24481 aatgcagcag ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga
    24541 gcaaattatc ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca
    24601 ggccaaaacc tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca
    24661 aaaagcatgc caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg
    24721 cttttagcac acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa
    24781 tcacattcat tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt
    24841 ccatgatcac accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa
    24901 tagttaagat catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt
    24961 tcgccagcac accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta
    25021 gaccaatcaa caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca
    25081 tccccaagac catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg
    25141 taggcaatct gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa
    25201 tttctcgtaa gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta
    25261 cgctcgacaa agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt
    25321 agcttctcta aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc
    25381 aacacattac ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca
    25441 cttaatgtac ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa
    25501 ctacggacaa gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct
    25561 tcagatgtca tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga
    25621 aggattcctt ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt
    25681 tcaccgacaa tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat
    25741 gttctcccac ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt
    25801 tcaagagatt ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct
    25861 cctatttttt ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca
    25921 ctgtcttttt gtttagagga agacaaccga tccagtctga tttgttgctt tttcttttta
    25981 ctggacactt tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat
    26041 cgttttttct ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc
    26101 ttttgagtga gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact
    26161 tcccagattt tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc
    26221 gtaccataat aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg
    26281 gttgttggct cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg
    26341 atctttcgtt cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg
    26401 aaatgactgg ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc
    26461 aggtagttga aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct
    26521 attagtttga gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct
    26581 tgctgataga cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt
    26641 agatttgtta tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa
    26701 caacaattat cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca
    26761 tcttttccta ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca
    26821 cctattctac tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac
    26881 atgtcttttg aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca
    26941 gtcatttttc cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag
    27001 ctactgaata gtaactgaaa accttttatt cgaacatagt gatcttttct ggtgctaaac
    27061 gaacagactc gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat
    27121 aacggtcttt atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg
    27181 gatttgtttc atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag
    27241 aatcgacttt gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga
    27301 tcgcagctaa ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt
    27361 tgtagatttc ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag
    27421 aagaagtatc attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt
    27481 cctcgctttc agcgaccaca aagcgccatg gtttcatatt gacagaagaa ggtgcagtcg
    27541 tcgcttcttt gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata
    27601 cgcgaacgga tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta
    27661 ttaacctctt tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt
    27721 gttagaaatt gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct
    27781 ttatggtcca tttcacaatg cttgattttc ttctgggctt tttctgttag cgctacatac
    27841 acttcccggt tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc
    27901 ttcagatgac gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt
    27961 agcaatggat tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg
    28021 atattttctt caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg
    28081 tgactaatct cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt
    28141 gacttgtcaa taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa
    28201 aaacgctctc aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa
    28261 tgataaagaa agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat
    28321 agctcttctg cagtagataa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag
    28381 aatcccgtcg aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga
    28441 caagatgttg tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa
    28501 taagttcaac caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg
    28561 atgcagggca gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat
    28621 ctccagccca tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta
    28681 ccagtttttt catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa
    28741 cttagacctt cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt
    28801 gtgtaatgat ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt
    28861 ggtgcttgcc aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct
    28921 ccggcattca atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga
    28981 gaagttgaag ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc
    29041 aatgtagaac cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct
    29101 ttgtaagagt tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa
    29161 gaagtcgtct tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt
    29221 ctaaaagcgg cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta
    29281 acagattggc cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc
    29341 aaagtactgt tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat
    29401 aagtcattgg cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt
    29461 tgagaattag cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac
    29521 aaatctgctt gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct
    29581 tctgcattct ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt
    29641 tctgttgttt cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac
    29701 tgcaaagtac tttctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt
    29761 gcgacagttt ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct
    29821 aaaagaataa cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg
    29881 aaacttttcc taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta
    29941 aataacattt gtttcttttt aataaatttg ctttttctgc ataaaaagac gtaaatgaag
    30001 aatattcttc tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta
    30061 attctctctg gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca
    30121 tgttgctgac agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat
    30181 cttcagatgt taatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt
    30241 cgctgatggt ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga
    30301 tgttattttg ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg
    30361 ttccgcaata aacggcggaa tcaggataaa caacatgtcc cgccacagaa tcagttgaaa
    30421 tgatatgccc agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca
    30481 cacccatgat gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg
    30541 gagcggttgg catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca
    30601 tcgctaaatc gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat
    30661 atagctcagc ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag
    30721 ctataactag tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc
    30781 ttgaagcgcc catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct
    30841 gttttgaaaa atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa
    30901 tccgttcgac aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga
    30961 catctttcgt aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg
    31021 gcagaatagt tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg
    31081 acaagcctct tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag
    31141 gaccaatcaa tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac
    31201 gattgataaa gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca
    31261 gcagaacagt caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga
    31321 tatctgctgg ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttccgctt
    31381 ggatctgatg gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt
    31441 gtttttcgat gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg
    31501 cttatgatgt ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg
    31561 tatgcttttt taaaaacagg agggaaaaaa tggatattcg tatactgacg tattttttag
    31621 cagtagctag agaaaaaaat atcagccgag cagcaaaatc tctgcatatc acgcaaccta
    31681 ctctctcaaa acaactgaaa gatttagaag aagaacttgg tgtgacactt ttcactcgag
    31741 gctcccgaga gatacagttg acggattctg gtcgatatct ttattcaaaa ggaaaagaaa
    31801 tcttgtcgtt ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc atctctggag
    31861 aaatctacat tggcggaggc gaaactaaag caatctcttt catttctgat agccttcatt
    31921 cgttgatcaa ggcgcatcct gatatccagt ttcatttata tagcgggaat gcagatgata
    31981 tcatagacaa actggatagc ggattacttg attttggact cgtaatcgaa ccgacaaaca
    32041 aacaaaaata ccagtcattt cgactgccag cccaagatcg ttggggactt ttgatacatc
    32101 ggaatcatcc tttagcaaag caaacagtca tccatccgca ggaactgaaa actgttcctt
    32161 tgatcatttc ccggcaaacg tcagtggaca gtcaactagc tgaatggcta ggtttcagca
    32221 tagatcagtt atcgatcgtc ggaacatata acttattgta caatgcttct ttgatggtcg
    32281 ataacgggac ttttggtgca ttagctctag atgggatcat caatacttat gggacggaaa
    32341 aagtattcat cccactcgat ccgccattac aagcttcact gaatctcatt tggaaaaaag
    32401 accagcctct ttctcctgct gctcgtaaat ttttagatgt cctaacggag aaatacagtt
    32461 aagtagagtt tttttccttc gctttcagct ttttaacaac gaataagtta agcaaaagat
    32521 ccaatgatta aataacataa gtttcttaat ttgttccttc cttttatttc tgataattcc
    32581 agtaaattac tataattata aaaaaagaat tgacaaaaca taactgtcta tataaaattg
    32641 attacgttaa caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg
    32701 aggaacatgg tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg
    32761 gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat
    32821 ctttaatcaa aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa
    32881 agggattgga tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat
    32941 atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga
    33001 acgcttttta tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa
    33061 gttgaatgaa catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat
    33121 taataacggt atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga
    33181 tgaattcagt attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata
    33241 tacttatgac gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa
    33301 caataaccat cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga
    33361 atcagaattg gaaacagata tccattcaga accattttca ctgaattatt caagactggt
    33421 gaatcattta cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac
    33481 ggaaatcgta gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat
    33541 ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt
    33601 ggccatccat atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga
    33661 ggaggaaaaa aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt
    33721 gcggtcttac ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt
    33781 aacgacattg tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct
    33841 ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc
    33901 ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct
    33961 actttattga atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta
    34021 tttattggta ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca
    34081 aaattaccga tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc
    34141 tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct
    34201 ttagtttctt ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc
    34261 ttcttcaacc gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg
    34321 tttgacgtag caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc
    34381 gtaacaggaa tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga
    34441 gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg
    34501 ttagcagcag cttttgcttc attctttact ggtgtaacag aaccacttga attctcattt
    34561 atgttcgttg cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca
    34621 gtgagtgcat tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac
    34681 ttcctatctt tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta
    34741 gtaacagcag tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg
    34801 actcctggcc gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac
    34861 aataaatatg cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta
    34921 acttcgatcg ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc
    34981 gatcaaaata aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat
    35041 gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat
    35101 ggtggtgctg caacacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc
    35161 ggtggaacga tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact
    35221 gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca
    35281 gaaaatggag aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa
    35341 catgctgtcg gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac
    35401 actgttgact taaaaggcga accattcact ttatatgtaa aagaaggtca aaaagtcgca
    35461 agaggtcaat tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg
    35521 gatatggtcg ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca
    35581 cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc
    35641 ttccccagac ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct
    35701 cctttttccg aataaaaacg gcgttctcct gccaactccg cggtaataag tccagccaaa
    35761 cgtaacatat gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct
    35821 tttttacaac ctctgataaa cggtgttcaa aaaccagttt atcggtatga agtcaaattt
    35881 atgcaaaaat gtggaaagcc attttcacaa aatttttctt catactcaaa actaatcggc
    35941 tttttcacaa cctctttata cattattcct gatccaatat ttcttttaat gctgcttcaa
    36001 gagaagttaa tggttttccc aatacttttt ctaaatcatc agaggaaata gctaactgat
    36061 cctttttaat atcatgttgg atcgctaaaa acatgtcagc tgcttcttga gggaatccgg
    36121 cttcttttag attttcgaca aacgctgcat cacttgcttc aataatttct aactttttac
    36181 ctgtcgcacg ctctaatgcc ttggctaatt cttcatagtc agttagattg ccggataatt
    36241 ctaagatttc tggagagtca ctttctacta gtacttttgc tgcagcttct gcatattcac
    36301 gtttcaatgc ccaacctgtt tttccatttc ctgcagcata aacgaattga ccagttttta
    36361 aagcctgccc aatgataggt aattcattct ctacatacca attattccgt aaaaatgtat
    36421 gggaaatacc tgaatcctta atcatttttt cagtgaattg gtgatctggc gctaatacac
    36481 tgatcgagtg atcggcatct gcaaaacttg tataagcgat ataagatacc ccagctgctt
    36541 gtgctgcctt cactacattc ttatgttcgg cttgacgatt tcctggaacg ccagagacaa
    36601 aaagcaatcg atctattcct ttcaacgctt ctttcattga gtctaaatca tcataatcac
    36661 caatacgtat attaaatcca gcttctttca gtgcttctcc tttttcttca ctacgtacta
    36721 gtacatagat ttcattttgt ggaaccatct tttttaagaa ttccagcgca taaccaccaa
    36781 aacctccagt tgctcctgtt actaaataat gcatatttgc cactccctat tctttttatt
    36841 tgttaagatt tacagctgta catttataaa ttacaccttt atcataaaga gtgtggtaag
    36901 ataagtcaag caaaaaaaat gaaaggatga caactgatga agtattctct gcaatttagt
    36961 gatgcaatcc atgtacttgc ctatattgaa atgtttcaag atacccgatc actttctagt
    37021 gagatgattg ccagtagtat cgaaacaaat gcagcaaatg tacgtaaaat catgagtcag
    37081 ctcaaaaaat ccggtttgat ctttacaaaa accggaaaac cgcagccttc ccttgccaaa
    37141 agtccagatc agatttcttt actagatgtc tatcgcagca tcgaaggtaa tacacagctt
    37201 atccaagtcg atccaaaaac aaatccaaat tgcgtcgttg gtgcgaatat ccaagaagct
    37261 ttagaagaaa gctacgctcg gctgcagaaa aaggcagaag aagaaatggc taagatcaca
    37321 cttgatagtc tgatacataa aatagccgtt ttggaaaaag aaaaaagacc agaaaacaca
    37381 ggcctcgtag agagattttt ataattctct ctactgtgcc tgcatttttc tgatcggaat
    37441 cgtctacata aattctttta ttctccgggg gacaaaagcc ttgtcatgcc cctttttcct
    37501 ctctaatgct caggtcaaag cgctgaagtc atgacgtctt gataaacggt gttcaagtag
    37561 ctgaccctcg gcattagttc tgaaaaatgc aaaatatgga aagctatttc ccatttttcc
    37621 tctctaatgc tcaggtcaaa gcgctacttt cacgacctct atattatcat ggtttattga
    37681 ctaccggtat ccagatttct cccatatatt tcccgttggt aactcccatt tcgactacag
    37741 catttggacc cccaacatag gcgtaatctt ctacttctct taaaacatgc ccaaaggccg
    37801 tattcgtcaa atcatttctt aattcttcag gcgattctgc taaagcgatt acaacaagat
    37861 acgttccttt tgggaatggg atgattcggg tagtgtttgg aacatcctga ctcgtttgaa
    37921 caccgacata gtacatcatt ttcccgttat agggttcgtt gactgtaaag aaataatgat
    37981 tttcagcagc ttgtttcaat gcatctattg ttccattacc gataactttt tgctcaaatg
    38041 cttgtttctc tgcagccaaa ccgacaaaat ccgtattgct actcttcaat tccacaccaa
    38101 tacccgtcac cgtaaaatct ggtttttctt cgatcctata ctttgccatt tctcccaccc
    38161 tttctattat attaagttca tcttacataa aattaaaggg aaatgacacg attacgctct
    38221 tttttctagg gatattttta ttttttaact atttttttgc tttcactgca atccaaatct
    38281 cactgtaaac attactgaaa tcctctcccc attttgtaaa agagatctcg ggagcttcga
    38341 ctaactcata atctgaagaa ggaagccatt ctgcatagat tttcccccaa gtatcttgta
    38401 aagtttgggg aaatggccct ttattaggaa aaatggccca agtgtgttct tctatagcaa
    38461 gcatttctaa atcagcgtac ggattttctt ttgtcgtagc aaagccaatc atgtgcatca
    38521 aactgccctt ttcttccaag cgggctccat caaatccata agaaacgttg attacttgat
    38581 ttggatataa atctcctaac ttatgcatct cttccctttg agtctccgtg atcgtctgcg
    38641 ctaacttttg tatttcttga ttgactcctt caaatttgac aggaactttt ttcgccacac
    38701 ctgccacata aaactgttct ttcttttcga tacgaaattc catcgatttt cctcctctga
    38761 catcaataaa aaatgtcaag cgtggaaaag atttttgtat gccgtatctg ctgatttctg
    38821 aaggcagata tcctgaccaa tcacgaaaag ctcttgaaaa accttcgact gtttcatatc
    38881 catatctaaa cgccgtttct gtcactcttg atccatctag gagatcgcga ttagccaaag
    38941 ccaatctacg gtttttgata tagtccatta gcgacatccc tgcaataaac gtaaagatgc
    39001 gttttaattg atattcagag attcctgtat tttcggctat tattttatag gaaagtcctt
    39061 ctgtcaaatg ttcttcgatg taatcgatca attggttcag atctttaagc atatttatct
    39121 tcctccttgc ttgccttttt atcatattat caaagaaagt caattacttg atgaatcagg
    39181 aaccaattta gtcagatgta gaaaatgttg tatggatcta aagaaaattc tcaagcagcg
    39241 tttcgtaacg gataattggt attatctgcc tatctatgtt ttacctttag aaaaataaat
    39301 tttggggaag acacattcta agtaaacaaa gcggtaaaaa tatcttttat tcccttagtt
    39361 aatccgctta ctacctactg tacaaagcaa attgttgttc aaactgctga tcagaacgct
    39421 gcttggattt ttctttgcga tacatcatac ctcttttatc aattggaaca attatttatt
    39481 tctaacaaaa tcacttcatt tttgtcagat aaataaaaaa caaaaaaagt tccatcttta
    39541 aaacacgcta ttcttttact ttttctcaca ttctctaaga aaagaaaagg agaatgaatt
    39601 tttgacaatt cgagcaaaag tgattatcaa atatgtaaaa tcagctactt ttttcttaaa
    39661 cgtttcttta tactaaatat aaataagtgt ttcatgaaag gagaactaga tgaataattg
    39721 gcttgagtat tttcctgaaa acgttttgga acgcggttac agctatcatc tgcacggttt
    39781 tgtccgtcat ttgaactata caagcaagta cttgtccgca acagtttctg gcacggaaga
    39841 ttataaggta gtgatcactt gggatgaaaa gaccaacatg acttgtgatt gtctatatgc
    39901 catagaagga aaaaaatgca aacacatggc tgctgtctta ttcgcttacg aagaacgtcc
    39961 aataaaaaaa tccaactact cactttccga actctcgtcc cttgtttcct ccgctagttc
    40021 ctctcttgta cgggagcttt taacagagat tcttatagaa catccgcaat ttatcgaacg
    40081 cttcaaagtt aagatgccat ttcatgcaat aaattattca gataaattaa caactatcat
    40141 ccataaatac gatcatatta tcaagaaaaa caaaaacagg aaaacagcga agttcatcat
    40201 ggagatgcga aagttcatcc aagaagcagt tgaatcgctc attcagcaaa atgcctatct
    40261 tcccgcattc gaattgatca atgaagtgat tgctactctc gaaacattct attgggagcc
    40321 tgaggatgaa cgaacccttc ttctgataga ggactgttat tatttatgga aagaattatt
    40381 agcagaagca cctcatgcag aaaaaaggca aatgttctct tggttcgtct gccaagtcga
    40441 tcatactgac gcctcttatt caaaacgcta ctccataaaa atcctgaaag aagactttcg
    40501 ggaaaaagaa ttttctaacc aaaagaaaaa aatagataaa aaaactaaag aaacggtaaa
    40561 gaaagacgat ttcaatgaaa aatagcaagt aaacgataac aaccatccta aatccacaag
    40621 aaatacagca gaccttgctt taaaaacacg aatcatgcaa aggatgaagt aaagaagact
    40681 taacgcgtac ccaatattag tcatttgact attaggtacg cgttttgttt tataagtcat
    40741 gacaaatttc cttcgttgct gggtagattt tccggcattg ttgatagatt ttatgatact
    40801 tttctacatt ttgtaagaga ggttccactg catctgtata ttggataaaa ttctgacaac
    40861 aactttcagg tgtgtcgaac cactctaatc tttaaagata aatattaaaa taacgattaa
    40921 taatattaac agtctttatt tttcaccacc aaaaacctat ctttgcatat actaaataga
    40981 cgtatataca cagataggtt tgtcttgtga aaaataatga ttatccttac caaaaaatgt
    41041 ttgtttttct acatgttcgt acttatcaaa aaatatgatc cttattgaaa ttactggcta
    41101 tttatattcc aacgacttct tgtgttaatt tttgttatct catagtaaat tatgtttcat
    41161 tttcacttaa tttagttgtc acgtgttcgt caattcgtaa aaacggtaaa tatataaaca
    41221 cacttgcggc tataattaat atttgtaaaa cagctgctcg ccagtctccg gctgttgcta
    41281 aaaatcccga tataactggt ggcgtagtcc aaggaatttg cactactaca tgattaatta
    41341 atcccacagc agttgcaaaa tatgctactg tcaaagaaaa aatcggtgca agcacaaagg
    41401 gaataatcag actgatatta aatacaatag gcaatccaaa aataataggt tcgcttatat
    41461 tgaatatagc gggagttacc gataatttag caaagtcctt ataatctttt ctacgactga
    41521 agataaatat tgcaatcaat aaagcaatcg tatttcccga tccacccatt accgcaaagg
    41581 tatctttaaa ggccatattg ataatatgtg gaatttgctc tttgtttgca taagctgcca
    41641 tattttcttg catattctga atcaaaaacg gatctaacaa tgaacctgat attactgctt
    41701 gatggatacc aaatccaaaa aatagattag caatagatgt aattaacaaa aatcctggta
    41761 aaccagttgt aattttactt aatggctctg taactatcgt ggtaattagc ttatttatat
    41821 ccatattaaa caattgaatg attataaatg acaataaagc aaacgaggta atcgtaataa
    41881 tgattgggat taatacatta aaagacttta aaacagcggg aggaatattt ccggatagct
    41941 gaatctgtag tttcttatta ttagaaagtt taataaataa ttctgtagcc gctaatccaa
    42001 caaatatacc tacaaacatt cctgaagctc ctgtatagga tataggtatc acacccgtca
    42061 cttcaacagc tttatccgca ttttcaggag taaacatcat ctgcataggt gttaatacaa
    42121 caaatgtcga aatagcaact aataccgctg caatatgatt tgtataattt ctgcttgcac
    42181 acatatgata agatactgct gctacaacta ataatgtagt tatgcttaat gtaccattgg
    42241 caattgaagt accaatttgt tgtaacgtgg tcaatgtact agtatcgaca atttccccca
    42301 tgaatcctgt tggttctaag atcactgaat taataagtgt cacaaatcct gctaacatca
    42361 taaatggcat aaatgtagca aacgcatctc gcaaagttct taaatggatc tgattcccca
    42421 acttagtcgc aactatacca aatttttcaa atacaatgtc cttcctgcta cttttgccca
    42481 tattttttct cctttatgct tattgtaatc gattactttt tccacccaaa taatacatca
    42541 cttatcaaaa aaagtaaata cttttaatta aatatatata tcttttttag tataaaaaaa
    42601 taagctctga aaaaataatt ttcccgagct tatttttcaa caaaaacagc ttcaaaaaca
    42661 aagtcttgat ataaatgtct cgataccgaa tactcgaaag gtttaccaga cgatagagca
    42721 acaactttgt ctatctcaat cataaattca cctggatcta tttttaatat ttttgcgtca
    42781 aattcattcg cagtatctcc tttgatacgt atgtgcgaag tctttatttc cagacccagt
    42841 tcatttgtga tataagcata tacagatttc tttaaatgtt ttggttccaa gcctggtatg
    42901 actgctaatg gcatgaatgt ttgctcaatc gagtatggtt gacaatcaat aaaacggctt
    42961 cgtacaatat tatatacata gtcttttaca ctgatactta atttatcagc tacttttttc
    43021 gatggaagtt caattgagaa agtatggatt tctgaatcta cgtgttcttc accaaccacg
    43081 ctaaacaaac cttcgtttgg accaagaact cgtgagttat caattggtgt tctgacgact
    43141 tcagtcccat acccacttct acttcgcaaa actccttcgg aaacaagtaa gtctacgcct
    43201 ttctttactg ttaacttgct tgtatgataa ataactgcca attcatctcc tgtaggaagt
    43261 ttctcaccaa ttttatactc tccatttaca atttttttct ttatatctaa gtaaatttcc
    43321 atatacttcg caattttttt caatggtgac cctccaatca aaaacatatc ttcatctata
    43381 ctttaacata aataaaccga taaaatccac atcaaaatta tacacacaat aaaaaagata
    43441 tatatattta aacaaaacta tttactttaa gttgtaagcg gtgttatcat taagtggaaa
    43501 caggaggtaa aaaaattatg acaactatga aaaagctaac ttttccagat gatttttggt
    43561 ggggttccgc ttggtcagct gagcaagcag aaggaaaagg agaaacagga aaagcagaga
    43621 ctatctggga tcgctggttt aaagaacaac cttaccggtt ttatcaacga atcgattcct
    43681 ctattacaac cgatcacatc catcaatata aagaagatat agaactaatg aaaaaaactg
    43741 ggcataactc ctttcgcgtt tctatttctt gggcacgaat gtttccaaat ggcggtaccg
    43801 gaaaagtaaa tcccaaagcc atttctttct atcgaaaatt attttccgaa atgaataata
    43861 acggaatcaa agtatttgcc aacttatatc attttgacat gcctgctact cttcaagata
    43921 aaggaggatg ggaatcacgt gaagtagttg atgcttatgt tcattacgct gaaacttgtt
    43981 ttaaagaatt tggtgactta gtttgtcatt ggtttacctt caatgaacca cttgggccta
    44041 ttttaggtac ttatcttgaa gattttcatt atccaaacct cattgacttt aaacgcggag
    44101 cgcaagcagc attcttcact atacttgcac acgcaaaagc aatacaagta tttaaagaac
    44161 tagctcttcc tagtaaaata ggcgttattc taaatttgag tcctactttt cctcgtagtc
    44221 aaaatcctgc tgatgtgaaa gcagcagaaa tcgctgattt attttatacg cggagctttt
    44281 tagatcctat ggttaaaggg aatttttcta aaaagcttat cctgttatta caagaatata
    44341 atcagcttcc tgaagattat acgaaagaag atcttgtatg tatctacgaa aataccgctc
    44401 aaattcttgg actaaactat tacgagccac gtcgcgtcaa atctcgttct tctatggtgg
    44461 atacatctag tccattttta ccagagtggt ttttcgaacc acatatcatg cctggacgac
    44521 gaatgaacga ataccgggga tgggaaattt acgaaaaagg aatttatgat ttgtgcatgg
    44581 atatcaagaa ccattatgga aacatcgaag catttatctc ggagaacgga atgggcgttg
    44641 cggatgaaga acgtttcatg gatgatacag ggcaaataat tgatgattat cggattgagt
    44701 atatcaaaga tcatcttgct tacttatgga aggcaataaa tgaaggatgt aacattaagg
    44761 ggtatcatct ttggacattt attgattgct ggtcatggat caacgcctat aaaaaccgat
    44821 atgggctggt ttcactcgac cttactactc aaaaaagaac aattaaaaaa agtggcgaat
    44881 tttacaagca attaagtgat acaaatggat ttagatacga tgtagacttg ttagttgaat
    44941 aatattctaa aattccactt gaagtgaaca cattagaaaa caagaataat gagaaatacc
    45001 aataacaaat actttttctt catacttcat ttatacttgt cagtataaga gactgtgaca
    45061 aatgtagtga tcctcagaag ttaggtctgg aaatctaact tgagaagatt tttttaaact
    45121 acgtccagtc ttttttattt gtttaaaatt gaagttgaca agtcttcatt aaaaatttag
    45181 aatattcaca agtttgacga caattatgaa aatttatgtc gtgctaaagg agttttgtta
    45241 ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac cgtttttggt
    45301 tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccgggtt tcatccgctg
    45361 gtcgtcacgt tgatgtccct gctgatctat gctggttctg cccaatttat cacggtcagc
    45421 atgcttgcta gccatagtcc actgctctcc atcgttctct cgacctttct agtcaattcc
    45481 cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa
    45541 aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg
    45601 aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat
    45661 ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg
    45721 aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa
    45781 gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg
    45841 attctggtat atgtcggact gatttttatc ccaagtaact tagtgatttt agttgtaaca
    45901 ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct
    45961 gactatttta ggttgtactt tagcaacttg gctttctagg atcctaccat ttgtcttact
    46021 gaaaaaattc gatttgccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat
    46081 tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat
    46141 caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag
    46201 cttattggtg atcgtcttgg taggaattct ttccctttcg ttgattcgct tattataaat
    46261 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
    46321 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
    46381 cctaatatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
    46441 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
    46501 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
    46561 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
    46621 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgttt gatttgatca
    46681 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
    46741 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
    46801 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
    46861 agttgattca aaatcgaacc gacgccccca gctccgttga tcactaagat ttttttcccg
    46921 cggttggcat tttcttctgg agtcagacca aatttttcaa ataacaattc ataagcggtc
    46981 aatgaggtca acggcaaggc tgcggcttct tcatctgaaa gattttttgg tgcaagagcg
    47041 acaatacgtt cgtcaaccag ttggtattct tgattgcttc ctgctcttgt cgttgtacct
    47101 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt catcacctac agcaacaact
    47161 ttcccgactc catcaaaccc taagatacgt aaagcatttc ttatcccgtt atcttggcga
    47221 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
    47281 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcgacaac
    47341 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
    47401 cttcatcaag ttggagcatc catacagttt tcatttcaat atatatagta tgctatttgt
    47461 tccactttta ttcatcataa ttattctctt acttaaagta aactatttgc acttttttgg
    47521 ttaaaaaaga ggttgcgaca agacttttgt cacaacctct tttaaacggt gttcaatcaa
    47581 ctgaccctcg gtattcgctc aaaaaacgac aaaatatgag gagctatttt tgtttggctg
    47641 ttcttattac ttgatgcagg acagcttttt cacgacctct aattcacggt gttcaagcaa
    47701 gaaatcattg ttcactgaaa agcgttggat gttctttttc aaaggtgtct aagatctctc
    47761 gtacttcatc agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa
    47821 atcctttgat atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt
    47881 ctgaataatg atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg
    47941 tatgttccct cggttctttt tcaaatgcat aaggattctt gaaaatcccc cggccaatca
    48001 tgatgccatc tacgccatat ttttcggcca attcttctcc tgtctggcga tctgggatat
    48061 ctccgttgat cgtgatcagt gtttgcggag cgatttggtc acgtaaagca atgattttag
    48121 ggatgacttc ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag
    48181 aaagattggc tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt
    48241 ccgtatagcc gatacgcgtc ttgacgctga caggcaaacc gccagcttta gccgcttcaa
    48301 tcaattgtgc cgctacttct gggcgaagga tcaagccact gccttttcca cgttctgcta
    48361 cattagggac tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag
    48421 caatgctcat ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct
    48481 gttcatcttc tgtaaaggtc aatcttcctc gtacactgtc ttttccatct ggatgacaga
    48541 aactgtctga gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga
    48601 catggcgaaa tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg
    48661 gcagctcttt ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg
    48721 aagtcgcaat ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag
    48781 tgaacagctt aaaagatgtt tctgattgag caaaaaaaga ctgtgacaaa aatcttatca
    48841 caattccttt tccggaataa atggtgttca aaagcttgca tctgcggtaa taagtccaac
    48901 caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca
    48961 gctttttcac aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca
    49021 acaaacgaca aaatatgagg aactattttc ccgtttgttt ctctaatact caggtcaaaa
    49081 cgctgatttc acaacctctt ttctaacttc tcaatcctct tcctttattg atcaaatacc
    49141 aagtaacggc gtacaaaaca acgatgaata gcacaagtac gatcatcgat gttgctactg
    49201 gcacatcgat tgttccaagg aatccgtaac ggaaaccgaa gatcatataa acgatcggat
    49261 tgactttcga aacagcttgc caaaatggcg gcaacataga gatggcatag aaaacgccac
    49321 ctagataagt taacggctgc aagacaaatg taggcacgat cgatacatcg tcataagact
    49381 gagcaaaaat cccgttgagc agtcctgcta acgaaaacaa gattgccgtc atcaataatg
    49441 taatgatgac catgaaccat gaatacacat gaagtggtac aaagaacaag gaaatgatcg
    49501 ttacgagaga gccgacgaga atactgcgac ccactccgcc aatcacaaag ccccaaatga
    49561 tcacatgcgt aggaacaggc gctaccaaga tttcttcgat gtttttctgg aatttttgag
    49621 agaagaagga cgaagaaacg ttggcatacg aactagtgat agcagacatc ataatcaaac
    49681 caggcacgat gaattccata tatgaaaaac cacccatgtc accgatccga ccaccgatca
    49741 tttttccgaa aataacaaag tacaaggatg tcgtgatcac aggcggaacc agtgtttgga
    49801 cccagatacg caaatagcgg ttcgtttcct tgatagctaa actttttaat gcagtaaaat
    49861 aaagattaaa catttttctc ctccgtatga ttattttctt ctgtgatttt caaaaacagt
    49921 tcttctaaac gattcgactt attcctcata gaaagaactt tgattccttg tcttgtcagc
    49981 tgatcgaaga ttccgttcac cccttgattt cgttcaactt cgacagcgat tgtctgttca
    50041 tcttcaaatg tacaagcgta tccttcaatc acaggtttgg tctcatatgg cgctaaatca
    50101 aagataaatg tttcaaattg cagtttggat agaagctctt tcatactagt attctcgatc
    50161 agttcacctg attggatgat cccgatgttg cgacaaagca tttcagcttc ttctaaataa
    50221 tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt ctcggagaaa ctcccacatc
    50281 tcgcgtctca attcaatatc gactcctgct gttggttcat ccaagatcag caaacgtggt
    50341 tcatgcatca atgccctagc aatcatcagt cttcgcttca ttcccccgga aagcatacgt
    50401 gcacggacat ttctcttttc ccaaagattg gactgcttca aatatttttc gctgcgtttc
    50461 atcgcttctt tgcgagagac accgtagtag cctgcttgat tcacgacgat ttgctggacc
    50521 gtttcaaagg gattgaaatt gaactcttgc gggactaatc caatctgttg ctttgctcgg
    50581 actagatccg tatccagatc ataaccgaag acattcactt ttccagatgt tttattgact
    50641 aaagaagtaa tgattccgat cgttgtagac ttcccagctc catttggtcc taatagcgca
    50701 tagaaatctc cttctttcac ggaaagatcg attcctcgca atgcttcgac cccacttcca
    50761 taaacctttt tcaaattatt tatttcaagc gcatttgcca tttatttcct atctccttat
    50821 agacatgatt ctttattgta tcatggaata cagcagatga aaaaacaact tggttccagc
    50881 gaaaaatcag gttgctgggc tcaggaaaat ctcctaatga aagacatcaa tcggacgaag
    50941 tcagacaacc ttttcagaaa aaacaaatcg aagataaagg attactattc atatgtgcaa
    51001 caaaaggagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
    51061 acggcggaag caaaagccaa gatttttgat ccaataaata ctttcgatca cttcgcctat
    51121 cccaatacat gccactgccg cttttcatca aataacgcgg cttccgtttt tgcggtttct
    51181 actaaagtct ctgggtaatc caaaactttc agcaaggcaa tcgcatttcg ggtccttgaa
    51241 ggtccttctt ttaatttgaa atcaaaactg attccttttc cttctgttac ttgttcttca
    51301 aagtggacat tctcacaata attctttaaa atctctgtca attcgatatc atgtgtggcg
    51361 acaaatgcta aagatggata ctccgcaagc caccgaacga ctgaagcgga agaagcaatc
    51421 cgttcgatcg tatttgtccc ttttaaaatc tcatctacga agcagtagca gcgttctttg
    51481 gaagccactt ggtccaacaa acgtttgatc gatttgattt ctgaaacaaa ataactgtcg
    51541 ccttcaaata aatcatcttc gattgccatg gaagtcaaca catgtccagg ttgcagggta
    51601 aactgttctg caatcgctgt ttggatcgtc tgtgctagaa tacagctgat ggctatactt
    51661 ttgacatacg tagattttcc tgatgcattg gaacccgtga ctaatgtatt cttctgccag
    51721 ttgaccggat tcactaccgc ttcttttaac aatggatgat agatatcttt cgctgtcaca
    51781 ccgccctcag taaactctgg tcggcaagtg atgggcatat aagtacggta attcaatatt
    51841 gcagcggcta cttctaattc tcccagcaat tcccacagct tgatggcttc agttgaatgc
    51901 ttttctaatt tagtgagaac gaaatgatag gagataaatg gcagcatgaa catgatgttg
    51961 acatattcaa aaatcaactc tgcttcactc ccactcttca tacgaaatga aaaacctaat
    52021 ttagggatgt ctcttaatgg tttagtttgc tgtttcagct cttcttgaag tggtgtcggc
    52081 agttttgcta gctgattggc ggctgaaatc gttgaaacca aataactcat gctattcagc
    52141 tctgtttcta gtgtttgttt cttcactaga taatagaccg tattgaatac taacccaccg
    52201 attgccaaat agattcctag tacttgtcct aaaagaagca aacccaaacc caccagcgga
    52261 agcaagccaa gaaaaataaa taaacctacg cgtccaatct ttttactttt tccatccgct
    52321 agatattgtt ttgcaaaatt atgatctttt tttcctaaac gagcgaattg atattggatt
    52381 ttttctctca gttgaggatt ttcctgataa aaagcaatca gtttttctaa ctgctgatct
    52441 tcaccaaaat caaagctgcg taaacgttga tacaacgctt ctgaacctac actagaatag
    52501 gtactgttga ttccttcgaa tacttcgaac atatccaggt cataccaagt aagatcatcg
    52561 atttcgctat cccatgaacg gaacttcttc tccgtgagcc atgcttcctt caagctttct
    52621 tctttatcaa atctcggttg ataaggaatt ttttcccatt tgctacgaac tgtttccttt
    52681 aatttcaaac gtgaaaaaat gtctaaagca actaataaaa taacagctgc aataataatt
    52741 aaaacgataa attctctttg acccatatcc ctctccgttc ctttatttgt tagctttagt
    52801 atagcactgt tgcgctattt gaaaagaggg taaaaaaaca cctttcaaaa taaaaggtgt
    52861 ttttttcttg tatattacat atttatctct catttatcaa aattgacaaa taatcatagg
    52921 gaggaattgt tgttaattct tgtttattct ccagtgcatt gacaactgtc ccaagggaaa
    52981 ggatagcctc aggtactatt ttttcttgtt ctgttggatt agctaagcga agaacaaatc
    53041 cttgttcatg aaaataagaa ggataaatcg atgatacaat taagtcttca ggtaattcaa
    53101 tcaatgaaaa tttacgagga acatcaagtt cattttgtaa tggccaaatc ttgttatcta
    53161 acctgtgaat aaatacattc agtttctgct tttgataaga aatagattgt tctaaccgtt
    53221 tttctgcctg ttttgccact ttgaactcat taaatcttcc ttcttctaca ttaaatccaa
    53281 aagtgacttt atactcacct atctcttgag ctagcggtgt ttccatcatg acatgccctt
    53341 catttgtcgt atctcctgaa gctcgcccag gtcgccaagc aagattaggt tttccaagtt
    53401 cccctgttgt tgaaaataag gttatgatta attgattttc tatacgttca tactctttta
    53461 aaccatctgc ataaaacgta aaataatgat cattttctgc cacagagaca ctcttatcga
    53521 atatttctaa attaacaggt ttctctacca tagtttctga ccaattcgga ggaatgacat
    53581 caggggtatt ttcaataaat ccgttttgaa tttgtgcaat tgatttttca gatttatcta
    53641 aaacattaat tgctaagcgc atacgatgcg agtaaacagt attatgaatt ttgagtgtgc
    53701 cttcaacgat ttgactttct cccagactga acgatatttc ataagatatt tcttccattt
    53761 cgggtttttc agataaacga tcttttaaat ctttaggaag ttgagctttt ccgtaaacaa
    53821 ctaatgtttc ttgtaaagaa tctttatata ctttagctgt ttctaatttt agagatagtt
    53881 ctgtatctcc ttctaatggt gaataatcat acgtatcgcc atcatttgct gaatcgatca
    53941 aatgaacgaa atcaagatat tgtctatctc caactatcaa attcaattgc ccatctttaa
    54001 aagaaagttt atatttattg ttacttatct cagttttgtc tatctcgatt gctgtttcta
    54061 atttttcatt atttttctca aattcaacaa cagtgtatcc tagtgctggc acatttacat
    54121 ttatttttat gtcaagttca taataagctg gttcatcaaa atattcgaaa cctgtagcaa
    54181 tcatccgata agcatttttc cgttctggaa aataaacttc tttttcaatg atattatagg
    54241 gactaccttt gaagtgtatt ttctttgaat cagctactac atgcacgatt ttcgatccac
    54301 tataattctc agtcgttgtg ttgaaaacaa ggacttcatt cgattttagc tttaatgcat
    54361 cagccatccg ttttacaata aggttttcga ttccgtcagc tatctcatta gcttctttga
    54421 ttcgatggaa aatatcttcc gcaacattat ctgaaacaca gccaccaata ctatcatgag
    54481 cctggttttg caaaatcttt ttccataacc gacgaagtag gcctttactg atatggatac
    54541 catttctttc tgcaatcagc atgagcggct caactcgttt tagaattttt tgttccaatt
    54601 taaaattttc tattttcatt tttgttctta ctgagccaat agaacgatga acacgagagt
    54661 aaaccggctg acgaagttca cctttatatt caggtaattc ttttttgttt ctgataactt
    54721 ctacataaga aggataatct gaaataatat tgtggaattt actgtattta ttcaacttct
    54781 ctacgacttg atcaaaattt aaaatcattg acttttgatc tacacccgaa ggtactaaaa
    54841 tatctttgtt ttttccttgc atatgaagca tttcaactgc ttcatccaac ttttcttttg
    54901 caaattcagg aatatcttta attgcgtcta aggtcatcat tccagtcata taaccaaatg
    54961 gaaaattcat ggcataaaca aatttatctc ccaatccttt ccagataaaa taaggagatg
    55021 gaaccatcgt ttcaaagttt aatcctcgcc aacacatgaa attatcgatt tctacttgat
    55081 gcaaaagtgt aggcaaattc gcattgaacc caaacgtgtc aggcaaatac cctaccatca
    55141 ttggatctcc atatttaagg atcgtatcac tgattccaat cattaagttt cttaaaattg
    55201 attcagcatc cactaataag gcatctgttt gtgcatacca cggtcccacg aatagttgtc
    55261 ctgttttcat cagtcgttgg ataaccttaa gcttttgcgg attgatctcc aaatactcat
    55321 cgacgattga agtttgtcca tctaaacaaa agtttacttt agggttcctt tccaattcat
    55381 ctagaacttc agtaaagacc tgatcagaca atacgatggc atcttcattt gtaaaatacc
    55441 attctctgtc ccagtgcgtg tgttgaatta tatgtgcatt taccatatca gattctccta
    55501 ctttccttct tctactgaat ttacttgttg ttctttttta tacttgatgg attttagtgc
    55561 attgaccatt aacgctgtaa ccactccccc aataacaatt gatactaaat ataaaggtat
    55621 attcgtaaca aaaggaattg ccataaaggt tgaaagcatt gtttcttgag taacaccaaa
    55681 aaatccattt agaccacatg ctactgcagt gcccaccacg atgcttggta ttactcgtaa
    55741 aggatctttc acggcaaacg ggatcgtccc ttcagtaatc cccacaaggc ccataatcat
    55801 agctgtcttt gcattatctc gttccgattc atcataaagt tttttgttta aaattgttgc
    55861 taaaaacatt ccaaatggcg gaatagcaat tcctaataac atggctgtac taggtccaaa
    55921 gcttccgctt gaaaaagaag caaccccaaa agcatatgcg attttattga taggtcctcc
    55981 catatcaaac gccatcattc ctcctaaaat agccgacatc aaagcagcat ttgctcctga
    56041 catgccttct agccaattag tcagtcctgt tgtcattgct tgaataggcg tagctagtac
    56101 ataccataat atgattccaa caactaatgt agtaacaaaa ggaatcagca taattgattt
    56161 tagactaata acttgcatcg gtacttttac ttttttcaat gcattagcta aatagccaca
    56221 agctaatcca gcaaccattc ctccaataaa tcccatacca agattttgac acatcatccc
    56281 agtgataaaa gctggtgcta gaccgggacg gtcagcaatt gaatatgcga tataggctga
    56341 aatcatcggt acgataaact gtaatcccgt ctgaccaatc attcgcagga ctccccagaa
    56401 tgtatccgtc tggtcccaaa cacctgtgcc accaaaaatg actgcaatag aagttagtaa
    56461 ccctgctgct acgacaagcg ggatcatata ggaaacccca ctttggaaat gatttagaat
    56521 ttctcttcct tgtttcttga attccattag taactccccc acgatatatt ttttatttgt
    56581 taattaatgc aattgcttca tcaatcgtct cttcaccatc ttttactacc tctgaggtcc
    56641 ctactgttaa aatcggttta ccgtcaaagc gacctcgttc tgaaacattt gtatctactg
    56701 caaatatgac aacatctgcc tcggaaacat ccgaaattga aagtttgttt tctatcccca
    56761 tagccccctg tgtttctact ttaatatcta accctttctt cttcgcaaac ttttctaaat
    56821 tagcttttgc catataagta tgggcaatcc ctgcgataca tgctgttact gctactattt
    56881 ttgtcatatg aaattcctcc ttttaaaatg atagagcttt taccaattct tttttatttc
    56941 tcgaatcttt tattctttta acaaactcgt catccatcaa tcttccagat aattctgcta
    57001 aaatacgtaa atgattagta cctttatcag tgtcttttat tgctaacaaa aagacgagat
    57061 caactggttg atcatctaat gcgccccatt caacagaccg ctttaacttg gctacagcca
    57121 ctgttgattc aattactgct aggcttttgc catgaggaat ggcgatattt ttcccaattc
    57181 ctgtggtcat atggttttct ctgtcaaata cgtctttttg aaattgtttg tggtcagaca
    57241 caaacccatt agtctctaac aatttcgata atttttcgac tacacttttt ttatcagatg
    57301 catttaaatc tatcactata ttatttatat tgattttttc actgatcatt tataagcctt
    57361 ctttcaatat ataaactaat ttttcaaacg acgaactcgt ttttaaattt gctaataact
    57421 catgattcga agcgatttca ttgaatgttt cataaatttt attcaattca ggttttactt
    57481 tttcattaag tgcaaaaaag aaaacagaat gaacaagtgt cccatcccaa tcaattcctt
    57541 ctggattaac gtagacacag attactggct tttttataaa atctacttcg atatgcggca
    57601 tagcaaaatc attcatcgct gttgaagata gtttttctct tttgattgct ccctctagta
    57661 tcccttcttt cgcgtaattt tcttttacca attttttggt aattatattt aaaacgttca
    57721 cttgattatc ctttatttgg tgggtaaaaa agcaaaatcg tttatctaat aaatttagaa
    57781 aagcatcttc tgtttgttca tttaactgta gatactgttt tacttttctg acatctgccc
    57841 ctaacatcaa aggactaaca acaataacag gaaaatttgt attttcaatc ggaatcgtgc
    57901 ttataacaag ttcatcagtg acttttctat cttgtagttc attgatgctt agtacatctt
    57961 ctatctcaac catatctgca aaaccgttat ttattctttg ctctaataat tttgatgttc
    58021 catatccgct actacaaact aatatcactt tcgttttgtc aggtttgtaa cgatccaaaa
    58081 gtgattgaat atggagtgtt acgtaagcga tttcatcttc attaaaatgt atacagaatc
    58141 tctcttcgat ttcctctaat aaatctacac ttatcataaa tgacctttta aaagaatatt
    58201 tgattttgtc cgtataaggg ttatagatat ttactcccaa tttgagtctt ttaactgctg
    58261 cattgagatg taatactaga ttattgatca attcttcatc aggataaaat tcatttaatt
    58321 taattcttac taactcttgt agctcaacac ccatatctaa ataggaaagc ttcgtttgat
    58381 ctaagtcgtg attcatagca tttttttcaa tcgaagtaat gtgaatgtca acataagctt
    58441 gttcatctat tggcacaata attgaaaatg ctttttcaat cattttgatt aaaataagtg
    58501 tattctttga gatttcttct tcagaattat tactacgtga taagaaattg cttgtttcaa
    58561 tcctttctat cgcaatagca aggtggatag ctaaagtttg aaattcataa tctgtaaatt
    58621 tcaattgtgt cttattcatg tatcgattaa gcaattcggc tacttttttc aatatgtcct
    58681 tatttaagaa attattcgct tcaccaatga aattgatatt caagtctaag agatctgttt
    58741 tactgttaga tgctgtaaag ccaccccaat aatagttcac aacttctgac atcaaccttc
    58801 ttttactttt ttcatcagct ttgatgcgca cgccatttcg atctgaaact aaggacatcc
    58861 catgcttgtt tagtcttttt ctaacatctt tcattgtgct ttctaccgtc gacctgctca
    58921 cgtataattc gtcagcaaga ttttgaatct ttagagaatc attagtattc aataatttag
    58981 caaacacata agttagacgg tcctccggag agtctgcgat attgatttta tgactggata
    59041 ataaatttcg aagtgtttct ttttttccaa taagcgaaac gcctttttta ggttgaacca
    59101 acatttgtac atctggcatt ttttggatta tttctttcaa aattaaaata tctttgctaa
    59161 ttgttttcgt agatacatat aattgttctg ataattcttt gtaagttgtg tgctcatttt
    59221 caatcaaaaa gtatagtatt ttcatttgcc gttcgctaaa ttcgatgccc attatcttca
    59281 cctcctgaat acagcttaag tgtacaatta ttatctctat tttcatacag aagaaagttc
    59341 ttgattaatg tagaagtttc taatattctt gattatttca aatcaataaa aaagcctgca
    59401 atatttatat cacaggcttt tttctctatt aaatatatat aaga
//
LOCUS       DAEGIQ010000004        57418 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000004 DAEGIQ010000000
VERSION     DAEGIQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.004"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            45..2486
                     /locus_tag="KMT45_000207"
     CDS             45..2486
                     /locus_tag="KMT45_000207"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBE7003698.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLQEWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2816..3520)
                     /locus_tag="KMT45_000208"
     CDS             complement(2816..3520)
                     /locus_tag="KMT45_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003699.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3707..3952)
                     /locus_tag="KMT45_000209"
     CDS             complement(3707..3952)
                     /locus_tag="KMT45_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003700.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(3969..4820)
                     /locus_tag="KMT45_000210"
     CDS             complement(3969..4820)
                     /locus_tag="KMT45_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBE7003701.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            4986..5990
                     /locus_tag="KMT45_000211"
     CDS             4986..5990
                     /locus_tag="KMT45_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBE7003702.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6231..6587)
                     /locus_tag="KMT45_000212"
     CDS             complement(6231..6587)
                     /locus_tag="KMT45_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBE7003703.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            6955..8142
                     /gene="tuf"
                     /locus_tag="KMT45_000213"
     CDS             6955..8142
                     /gene="tuf"
                     /locus_tag="KMT45_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE7003704.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8330..10921)
                     /gene="mprF"
                     /locus_tag="KMT45_000214"
     CDS             complement(8330..10921)
                     /gene="mprF"
                     /locus_tag="KMT45_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBE7003705.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10947..11708)
                     /locus_tag="KMT45_000215"
     CDS             complement(10947..11708)
                     /locus_tag="KMT45_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE7003706.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11875..12507
                     /locus_tag="KMT45_000216"
     CDS             11875..12507
                     /locus_tag="KMT45_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBE7003707.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            12566..13426
                     /locus_tag="KMT45_000217"
     CDS             12566..13426
                     /locus_tag="KMT45_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBE7003708.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEKYK"
     gene            13611..15926
                     /locus_tag="KMT45_000218"
     CDS             13611..15926
                     /locus_tag="KMT45_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBE7003709.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16108..17949
                     /locus_tag="KMT45_000219"
     CDS             16108..17949
                     /locus_tag="KMT45_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE7003710.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            17973..19082
                     /gene="rpoD"
                     /locus_tag="KMT45_000220"
     CDS             17973..19082
                     /gene="rpoD"
                     /locus_tag="KMT45_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBE7003711.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19302..21401
                     /locus_tag="KMT45_000221"
     CDS             19302..21401
                     /locus_tag="KMT45_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBE7003712.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21597..22460
                     /locus_tag="KMT45_000222"
     CDS             21597..22460
                     /locus_tag="KMT45_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE7003713.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22565..23038
                     /locus_tag="KMT45_000223"
     CDS             22565..23038
                     /locus_tag="KMT45_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBE7003714.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            23134..24021
                     /gene="xerD"
                     /locus_tag="KMT45_000224"
     CDS             23134..24021
                     /gene="xerD"
                     /locus_tag="KMT45_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBE7003715.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            24040..24411
                     /locus_tag="KMT45_000225"
     CDS             24040..24411
                     /locus_tag="KMT45_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE7003716.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            24381..25178
                     /locus_tag="KMT45_000226"
     CDS             24381..25178
                     /locus_tag="KMT45_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBE7003717.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITLDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            25162..25731
                     /gene="scpB"
                     /locus_tag="KMT45_000227"
     CDS             25162..25731
                     /gene="scpB"
                     /locus_tag="KMT45_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBE7003718.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            25737..26453
                     /locus_tag="KMT45_000228"
     CDS             25737..26453
                     /locus_tag="KMT45_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBE7003719.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      26535..26660
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            26783..27394
                     /locus_tag="KMT45_000229"
     CDS             26783..27394
                     /locus_tag="KMT45_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBE7003720.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            27772..29295
                     /locus_tag="KMT45_000230"
     CDS             27772..29295
                     /locus_tag="KMT45_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBE7003721.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            29532..30872
                     /locus_tag="KMT45_000231"
     CDS             29532..30872
                     /locus_tag="KMT45_000231"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBE7003722.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            31026..31763
                     /locus_tag="KMT45_000232"
     CDS             31026..31763
                     /locus_tag="KMT45_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBE7003723.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(31928..32149)
                     /locus_tag="KMT45_000233"
     CDS             complement(31928..32149)
                     /locus_tag="KMT45_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBE7003724.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            32180..33211
                     /locus_tag="KMT45_000234"
     CDS             32180..33211
                     /locus_tag="KMT45_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7003725.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            33198..34601
                     /locus_tag="KMT45_000235"
     CDS             33198..34601
                     /locus_tag="KMT45_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBE7003726.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLCHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            34667..35302
                     /locus_tag="KMT45_000236"
     CDS             34667..35302
                     /locus_tag="KMT45_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBE7003727.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            35351..36031
                     /locus_tag="KMT45_000237"
     CDS             35351..36031
                     /locus_tag="KMT45_000237"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBE7003728.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVAKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            36166..37395
                     /gene="rpsA"
                     /locus_tag="KMT45_000238"
     CDS             36166..37395
                     /gene="rpsA"
                     /locus_tag="KMT45_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBE7003729.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPAGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            37577..38887
                     /gene="der"
                     /locus_tag="KMT45_000239"
     CDS             37577..38887
                     /gene="der"
                     /locus_tag="KMT45_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBE7003730.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPISGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            39130..39405
                     /locus_tag="KMT45_000240"
     CDS             39130..39405
                     /locus_tag="KMT45_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBE7003731.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            39640..40686
                     /locus_tag="KMT45_000241"
     CDS             39640..40686
                     /locus_tag="KMT45_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003732.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            40688..41947
                     /locus_tag="KMT45_000242"
     CDS             40688..41947
                     /locus_tag="KMT45_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBE7003733.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            41953..42534
                     /locus_tag="KMT45_000243"
     CDS             41953..42534
                     /locus_tag="KMT45_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBE7003734.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(42616..43491)
                     /locus_tag="KMT45_000244"
     CDS             complement(42616..43491)
                     /locus_tag="KMT45_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBE7003735.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            43639..43971
                     /locus_tag="KMT45_000245"
     CDS             43639..43971
                     /locus_tag="KMT45_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBE7003736.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            44083..45291
                     /locus_tag="KMT45_000246"
     CDS             44083..45291
                     /locus_tag="KMT45_000246"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBE7003737.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            45445..45900
                     /locus_tag="KMT45_000247"
     CDS             45445..45900
                     /locus_tag="KMT45_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003738.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            46262..46537
                     /locus_tag="KMT45_000248"
     CDS             46262..46537
                     /locus_tag="KMT45_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003739.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            46550..48628
                     /locus_tag="KMT45_000249"
     CDS             46550..48628
                     /locus_tag="KMT45_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBE7003740.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAESTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            48788..50674
                     /locus_tag="KMT45_000250"
     CDS             48788..50674
                     /locus_tag="KMT45_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBE7003741.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            50686..51633
                     /locus_tag="KMT45_000251"
     CDS             50686..51633
                     /locus_tag="KMT45_000251"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBE7003742.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            51653..52180
                     /locus_tag="KMT45_000252"
     CDS             51653..52180
                     /locus_tag="KMT45_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBE7003743.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(52243..52896)
                     /locus_tag="KMT45_000253"
     CDS             complement(52243..52896)
                     /locus_tag="KMT45_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBE7003744.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            53028..53870
                     /locus_tag="KMT45_000254"
     CDS             53028..53870
                     /locus_tag="KMT45_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBE7003745.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            54028..54924
                     /locus_tag="KMT45_000255"
     CDS             54028..54924
                     /locus_tag="KMT45_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBE7003746.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            54927..55640
                     /locus_tag="KMT45_000256"
     CDS             54927..55640
                     /locus_tag="KMT45_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBE7003747.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            55660..56178
                     /gene="msrA"
                     /locus_tag="KMT45_000257"
     CDS             55660..56178
                     /gene="msrA"
                     /locus_tag="KMT45_000257"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBE7003748.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            56175..56402
                     /locus_tag="KMT45_000258"
     CDS             56175..56402
                     /locus_tag="KMT45_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBE7003749.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            56554..57105
                     /gene="lepB"
                     /locus_tag="KMT45_000259"
     CDS             56554..57105
                     /gene="lepB"
                     /locus_tag="KMT45_000259"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBE7003750.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(57188..57370)
                     /locus_tag="KMT45_000260"
     CDS             complement(57188..57370)
                     /locus_tag="KMT45_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003751.1"
                     /translation="MIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVY
                     SFIKTLEARKVFKRAV"
BASE COUNT        19440 a         9862 c        12057 g        16059 t
ORIGIN      
        1 tttttacagc aaagaatggt atatttttgt aggaggtggc ttacttgaaa gaagtcgtta
       61 tgatagatgc tgcaagaacg ccgataggaa agtatcgcgg aagtttaagt ccatttactg
      121 cagttgaatt aggcacactt gtgactaaag gattattaga taaaacaaaa ctgaaaaaag
      181 ataaaatcga tcaagtaata tttggaaatg tcctgcaagc tggtaatggt caaaatgttg
      241 ccagacagat tgctttgaac agcggtctcc ctgtagatgt cccagcaatg acgatcaatg
      301 aagtttgcgg atctggtatg aaagcagtga tcttagcacg ccagctgatc caattaggtg
      361 aagcagaact tgtgatagcc ggcggaacag agagtatgtc tcaagcacct atgctgaaac
      421 agtatcagtc agaaacaaat gaatatggtg aaccaatttc cagtatggtc aacgacggat
      481 tgactgacgc attttcaaat gcacatatgg gattaaccgc agagaatgtt gcaacacaat
      541 tttctgtgag cagagaagag caggatcgct atgccttgtc gtcccagttg aaagcagcac
      601 atgctgtcga agccggtgta ttttctgagg agatcatccc agtcaagatt tctgatgaag
      661 acgtgttatc tgaggatgaa gcagttcgtg gaaatagtac attggaaaaa ctgggcacgt
      721 tacgtacagt attctcagaa gaaggaactg taacagcagg aaatgcttcc ccgttgaatg
      781 acggtgcctc tgtggtgatc cttgcatcca aagaatacgc agaaaataat aatctgcctt
      841 atttagcaac catcaaagaa gtagcggagg tcggtattga cccaagtatc atgggaatcg
      901 ctccaatcaa agccattcaa aaattgacag atagatcagg aatgaattta tcaacaatcg
      961 atctttttga gatcaatgaa gcatttgcag cttcttctat cgtagttagc caagaactgc
     1021 agttagatga agaaaaggtc aatatatatg gaggagcaat cgcattaggc catccaattg
     1081 gtgcaagcgg tgcacgtatc ttgactacat tagcttacgg attgcttcgt gaacagaaga
     1141 gatacggtat tgcttcatta tgtattggtg gaggtttagg cttggcagtc ttgttagaag
     1201 cgaatatgga gcaaacacat aaggatgtgc aaaaaaaaaa gttttatcag cttacgccat
     1261 ctgaacggcg cagtcaatta atagaaaaaa atgtattgac acaagaaact gcgttgattt
     1321 ttcaggaaca aacactttct gaagagctct ccgatcacat gatcgaaaac caagtgagtg
     1381 aagtggaaat cccaatgggg atcgcgcaga attttcaaat caatggcaag aaaaaatgga
     1441 ttccgatggc aacagaggaa ccatctgtca ttgcagctgc aagtaatggt gcaaaaatct
     1501 gtggaaatat ctgcgcagaa acacctcagc ggctgatgcg agggcaaatc gttctatcag
     1561 gaaaatcgga gtatcaagca gtgatcaatg cagttaatca tcgaaaagaa gagctgattc
     1621 tttgtgcaaa tgaaagttat ccttcaatcg tgaaacgcgg gggcggtgtt caagatatct
     1681 ctactcggga attcatgggg agtttccatg catacttatc tattgatttt ttagtggatg
     1741 taaaagatgc aatgggcgct aacatgatca actcgattct tgagagtgtg gcaaacaagt
     1801 tacaggagtg gttccctgaa gaagagattc tattcagtat tttaagtaat tttgctacag
     1861 aatcattggc ttctgcctgt tgtgaaatcc cattcgaacg cttagggaga aacaaagaaa
     1921 tcggtgaaca aattgccaaa aaaatccaac aggcagggga atatgcgaag cttgaccctt
     1981 accgtgcagc aacgcacaac aaagggatca tgaatggtat agaagctgta gtggcagcta
     2041 caggaaatga tacacgtgcg gttagcgctt cgatccacgc ctacgcggca agaaatggat
     2101 tataccaagg attaacagac tggcagatca aaggggataa actagttgga aaactaacgg
     2161 tgcctttggc tgttgccaca gtaggcggag cttccaatat cctgccaaaa gcaaaagcat
     2221 ctttagctat gttagatatt gattcagcca aggaactagc acaagtaatt gctgcagtag
     2281 ggttagctca aaacctggct gctttgcgtg cattagttac agaaggaatc caaaaaggcc
     2341 acatgggact tcaagctcgt tcattagcga tctcaattgg ggctattggt gaggaaatcg
     2401 agcaagttgc gaaaaaatta cgtgaagcag aaaagatgaa ccagcagaca gctatacaaa
     2461 tattggaaaa aatccgggaa aaataaaaaa gcaatatctt tagaagaata aagcaagatt
     2521 tacagttatt ttatttactg cagatcttgc ttttttctct atcagaaaat atgtttatta
     2581 ttagaatttt ttatagttaa ggttaggcat cttaagaaat caggcaactc caagatatct
     2641 tcaggtagaa gagaagtcgc ctaacattgt aggatcatag gtgatttgtt ggacaggtca
     2701 agctgatatt tgagcctgat gttagaagga atttcttttt acttgctgta gaaattatta
     2761 tatgactaaa aaggctggga ttttaaatcc cagcctttta tgacaaatag aatttttatc
     2821 gatcagtaat cgctgtcgct gcaaattgtt cgatcaaagg aatcaaagtc aatttgactt
     2881 tgtatttccg ttcgagtgct ttttcgataa cgaactcatt ttcagtagtc atagaaatag
     2941 ttgctaattt acgaggttca tcagcatttt tttcattaat aactacttca tgttcatttt
     3001 gttctgtgta aaatttagga tactcagcaa cggcttttga aaaatcagca aaacttaaag
     3061 cggcattcgt atcagttgag cgtgtatttt cagttgtgtc cgcttttggt ttttccgttt
     3121 tttcttctgg cacactgact gtttcttctg taactggtgt cgcaacagct tgcgcagttg
     3181 gttcatcgtt tttcaatcgt tgatcattcc agtaagacac gatttcaata agaagattgt
     3241 cacggaatcg ttcgaaaatc ccgttttctc ctgaacgaat tctctttaat acagtattga
     3301 agttgtcatc gattggcacg ttgatccgtg tatcttcatt tgttttccca gctaatccga
     3361 tcgttaagat ttgacggttt ttgggatcaa gcatgacttg taacggcaaa ccataacgtc
     3421 gcaagctctc ttcttctaga gaacggaata attgtcctct tacaccatcc aatgaaggac
     3481 ggaattgatt taataatttt tgtttctgtt ttttcttcat atgttctctc cttcgctcat
     3541 accctttcga ttataagaaa aaaacagcca aatgaaaagg agtaatgcaa aaaaacttgc
     3601 gtatacgcaa attacaacag aaaaatttgc gtattttccc gttttttgaa agaaaaataa
     3661 aaaatggaac ggattatgta tatatacata atccgttcca ttctttctat tttttcggta
     3721 tcaaggcaaa aacaggtagt tcaggtgaat aaatgacttc atgttcgtgc cagggtttga
     3781 ccattagcga atgatcgaac cgttttttca gttcttcgat gatttgatca tgtgtccagt
     3841 ttcttgcagg aaaatgttgg attttctctg catcgatcaa aaagacaaaa tcggaatgaa
     3901 aagtctgggt aacttcttcc gagagagaat tcaagcgttc gattggatcc ataaaatcac
     3961 ctttcgactt aaaattgggt agtatctggg tcttttgctt tgccgactac accatctaac
     4021 tgatcgattt gtttcatttc ttcttctgtt aattcaaaat caaataattc agtattttct
     4081 ttgattctgc ttggtgtcac tgacttagga agaggaagga attcgtgttg cagcgaccaa
     4141 cgcaaagcaa cttgtgcaat cgtcttgtca tgtgcttcag cgatttgttt catttcaggc
     4201 acatcgaaga ttttaccagt gcctaatgga ctatatgctt cgaggatcat atcatgtttt
     4261 tttgcatact caactacagt tgtctgtaat tcgcctggtg caaggaaaac ttgattgacc
     4321 atcggcataa tttttgctgt ttttgctagt gtatctagat ggtggggtaa gaaattgctc
     4381 acaccgattg ctttgatttt acctgcttca tacagttctt caaatgctcg ccatgtatct
     4441 gcatttgctt gttcccaatt atcacgaaaa gcaacagggt ttggccagtg gatcaaaaac
     4501 agatctaaat aatctgtttg gagcttcttc aatgattctt caaaagaaga catcacaagt
     4561 tcatagctgt ggtttgcatt ccataatttt gttgtcaaga atatctcttc tctaggaatt
     4621 ccactttcct taatcgcttg gccgacgctt tcttcattct tgtatccttg ggcagtatcg
     4681 atatggcgat aacctgcagc cagcgcttct ttaacagagg aaacagcgac atccccatca
     4741 ggtgtttgcc atgtgccgaa tcctactacc gggatttcgt aaccatttga tagacgataa
     4801 gtgcttgtta atgaattcat actttcagct ccttttttat tcgttcaact ataagtgtag
     4861 cgcatttttc gaaaaaaaga atccattttg tttctactct tgaaattttt gtttaaaaga
     4921 aaatgatttt gattctggta tttttgattt tttttccgta aactaagaca gaagggaatg
     4981 agaaaatgga tatctattta aaaaaagcaa ttctgcatat tattgacaga gaaactggtt
     5041 ctccagtctt ttcacaaaaa gagctggatc tgacaaaaga atatattcgt gattttcttc
     5101 aaaagaagat ccaaaaaatc tcttctgctc aaacaaaaac tggacaacta gcagaagaaa
     5161 gcatgatcgt tgaatcactt aaaatcgctc aagaaaattt tgtggaagca agtgaagcaa
     5221 tcgttcagcg gtggttcgat atctaccaag aaagtgaaga agcacctagt gcagatgtat
     5281 ttgttgtctt gtatgaatta gatacagtga tgtacttatc tttattgaaa gtcaattatc
     5341 gtgaggctta cactcacttt gttgaagcag acgaagcagg gatcgacaac cgcttgattt
     5401 tgaatcgagc aattctaggg gcgaaaacac aaaaagcaga tgaagcattc gctgtgaatt
     5461 tgactgattg gacctacgaa ttaattgaaa aaaaatatga gttttctggc aaaaaagagc
     5521 tttatttttc tggaagagtg atcgaaagtg ttcctgcacc ttctttagaa gaaaatgtaa
     5581 aagtgatcca aaaagtagcc aagcatcttg gaaaaaaatt tgagacagaa gaatttgata
     5641 tcatggctga tttgaaagaa gctgtctatg atacgataga agaaaaaggt cggttggatc
     5701 atgagatgat tgcggaaaaa gtcttcaagg aaaacatcac ggcaaaaatg gcttttaaag
     5761 aagaagtaca agagcaagga tttgtcccag aagcacctcc tgtaaaagaa gtacaagaaa
     5821 tttctgagaa aaaatttgga aaacaaaaat tcaagctatc aaacgggatc gaactgatcg
     5881 taccagtaga tgtatatcgc aacccagatt tgattgaatt tgtgaataat ccggatggta
     5941 cgatatccgt gatgatcaaa aatgtggatg aagtattgaa tcgtttataa aaatgactag
     6001 aatattaggt gttcaagtac cagtgatcag ttaaatgaat tgtgtcaaga gaaggatcgg
     6061 aaaagacgaa tataacaaaa aaagagcgtg agacaaaagt aaagaacact tttgtctcac
     6121 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcttatttc ttggagttaa
     6181 acgcttctgt cccagcctct ttttatctaa taaaatttta agaccattta ctaattatcg
     6241 attttttctg ccgcattttt aggcacttca gaagccttta tttcttccca gcttaataca
     6301 cctttacgag gattgacttt gagtttaaga tacgcattca tctttaatgg atgttctctt
     6361 tcttcgcgaa gcgtcacctg ttttttttca ccatctttat tataagccgc ttgcttgtag
     6421 gtataagtcg tgaatttctc tccgttatct gcttgggtag ctgacatttc gccattcgtc
     6481 gtgatttttg tataataagc ttcgccgccg taaaaataat gataagctgc aaaacttccg
     6541 ccgagaaata tgcctaaaac gacaagtacg ccaattaatt ttttcatgta cttcacctct
     6601 ttccttatta ttaacgaaat agaagatttc gtctattgaa agttgttggc aacagctgaa
     6661 ttttaaactt ttcctcattt tgttcttatt gcttcaaaag aaagttaagg actatcttga
     6721 agtttttctc aaaaaacaca tttttttatt tataaaaggt acttttttcg cgtataagac
     6781 agacgagtgc tcttagttga ctttgaccaa tattgacgta taataagctt agttcgaatc
     6841 ttacaaaaag taagtcggac gtattctttt tgatttagtc aaaaaaagac tataataaaa
     6901 aggtgagagc tgaaattttt cagtgatcta tattatttag gaggtttgta aattatggca
     6961 aaagaacatt atgacagaag taaaccacac gttaacattg gtacgattgg acacgttgac
     7021 catgggaaaa ctactttaac tgctgcgatc actacagtat taggcaaaaa aggcctagca
     7081 aatcctcaag actatgctag tattgatgca gctccagaag aacgtgaacg tgggattacg
     7141 atcaataccg cacacgtaga atatgaaaca gaaaaacgcc actacgcgca tattgatgca
     7201 ccaggacacg cggactatgt gaaaaacatg attacagggg cagcacaaat ggatggtgca
     7261 atcttagttg tttctgcaac tgacggtccg atgcctcaaa cacgtgaaca cattttattg
     7321 tcacgccaag ttggtgtaaa atacctgatt gttttcttga acaaagttga tttagtcgat
     7381 gatgaagaat tgatcgattt ggtagaaatg gaagttcgcg agttattgag cgaatatggt
     7441 ttcccaggcg atgacactcc tgtgatcaaa ggttccgcat taaaagcatt gcaaggcgat
     7501 ccagatgctg aagctgctat tatggaattg atggatacag tagatgaata tatcccaaca
     7561 ccagaacgtg atacagataa accattacta ttgccagtgg aagacgtctt ctcaattaca
     7621 ggtcgaggaa ctgttgcctc aggtcgtatt gatcgtggtg ctgttcgtgt cggtgatgag
     7681 gtagagatcg tagggatcaa acctgaaaca caaaaagcag ttgtaacagg tgtagaaatg
     7741 ttccgtaaaa cgttagatta cggggaagct ggggataacg taggcgtgtt gttacggggg
     7801 atccaacgtg acgatatcga acgtggacaa gtacttgcta aaccaggttc cattactcca
     7861 catacaaaat tcaaagcaga agtgtacgtg ttgacaaaag aagaaggtgg acgtcatact
     7921 ccattcttca acaactatcg tccacagttc tacttccgca caactgatgt tacaggaaca
     7981 atcacattgc cagaagatac agaaatggtc atgcctggtg acaacgtgac gattgatgtt
     8041 gacttgatcc atccaatcgc tgttgaaaac ggaacgacat tctcaattcg tgaaggcgga
     8101 cggacagtag gttcaggtat cgttacagag atcgaagcat aagaaacgcg actcgaatat
     8161 tttggtaatg aaaaatgttt aaaataaaag agcggtccaa acaggatttc tgtttggacc
     8221 gctctttgtt cttcaattgt ataagaagag attgtagaag catgaacttt cgttttactt
     8281 cagtctcttt tattcatcat gagagtgttc atctatcggt atgcagtttt taatactttc
     8341 ttcgtatcca ttttttcaaa ccacttattc taccttgtgt ctgctcaact ggcgcattat
     8401 caataatcag caaagcaatc ataacatatg caatccaact atctctagaa tatagagtat
     8461 aacgtgagac ccagtttgta gcgtacttgc ttttatattc tcttaatcca tggaaagaat
     8521 aaaaatgaga accaaactcg taaaccaagt atgcaatcct ttcctgtaaa aaactctttc
     8581 tggaagttcc gacatttgaa agaggggcca tccctaaatt gaaccattgg atatgttctt
     8641 ctttcatata ggcaaacaaa tgcaagaaca agaaatccat actgccagaa ggtgcttttt
     8701 tagaatcata tcgcatcaga tcaatcgttc ctactttatt gttcgtgtaa gtaggcatga
     8761 tcgttgcaaa agcgacaatt tcttcttggg tatttttgac aactgcaata ggcgctcttt
     8821 gtagataatc ttcagagaaa aagcctaaag aaaacccttt ttcctttcgt gaacccaacc
     8881 aattatcaga gatatttttg aaagtactca tttgctcagt agaaaatggt ggttgtaaga
     8941 catcaaatgt aaatccttct cgttcgatcc gattcaagac agcacgagtg cccttcattt
     9001 ttttccctga agttgtaaaa ctattcaggt ctacataagc ttcttccccc atcttaatga
     9061 aatcgtagcc gaattcatgc aaaatcataa caatctcttc gcttgtttcg tagaaaactg
     9121 gtaaatagca tagtcgatct gtttcttcga taaaagcttc gattgcttct ggaaaatctt
     9181 ctttcttacc agaaggatca cccattacaa tacatttatt attgaagcaa gcgaattgga
     9241 gaagaacagt tggttcaccg tctttttcat aggcaaacat ccgcttatct tttaagaaga
     9301 tcaattgact atcggagttt cctccataag ttgtaaggat cttcagggcc ttttcttcat
     9361 taaaggcttc gccaatctgt ttcttttctc cttgaaggaa atgaacaaat agaacgatca
     9421 tgaaactaac agcgatgatg gccaacaatc ccgaaaacca aatcttttca gaaggaaata
     9481 agaaaaagga aataaaatgg tgacgccgat gagggaaatc cggcagattg tataccccaa
     9541 taacgatgta aagcaaggac agtgcaccaa taatgattcc atcgatcgta cgccactccc
     9601 atgaatagat caattgttcc cgaaataatt cgtttttaga agcaagaatg atcagaagca
     9661 gaatagacaa gaaaaccacc ggtgtgaaac taaaatcact taataaaaca taaagtaaag
     9721 ctagaacaat caagaagatt gtaggcaaat atgctcgttt aacacgtgct gcgatccctc
     9781 tgcccataat gattaaaaga aatcccaata gtatactagg aaattggata ataaaatgaa
     9841 atttcaatgg gttcaaacta tgaagccagc gaaactctgt aaaagcttgc gggatagtgg
     9901 cagaaagtac tagcatgatt ccagaaaagt ataataagac aaccacgatt ttatgcgcaa
     9961 tttctgttgc taattgttta gggataccag aataacgctg atcaaaagtg gaaccgatat
    10021 ttttgaagaa aaagacgatt ccaatcaaaa acggaatgat gtagtagaac aggcgataga
    10081 gcaagatcca cacaacgact atttctcttg gcacaccaat agcggacaac ccgataatca
    10141 tcattacatc gaaactccca atttctcctg gaatcataga cacgattcct atcactgaag
    10201 cagcaatgaa taatggaatt gtttgccaca gatcaatccg gatttccatt aaataaccaa
    10261 cagaaaggaa actaaacagg acaccggtcc attccaacaa tgaaactagc aatagctgtc
    10321 cttgagtttt aagtgaggta ccaccaagga atcctttctt tttgaaagta gtaaataaaa
    10381 agacgacagg aaaatataga ccgccgccga tcaaccatat ccaatattgc ttcaaaaaag
    10441 gagcaactgg cgtgaagact acaagtaaaa aagaaagcag actgtaaata gaaagtccag
    10501 ccatcaaaaa caagaaaact ttcgataatg cttggatcac gtttctgctc tctttctttt
    10561 gtccatatag ttcagagcga agccccacac taacaaaccc gccgaaaccg gcaatattgt
    10621 tgatcgtatt gatcagccag cttgtctcaa acagataacg aggattctgc ttttgttcaa
    10681 gcatccgatt caaaataata tcataaccaa ccataggtaa cacagagata agaccaatca
    10741 atagcattag tccgcttttc catacaggta tagtggcaaa cgtttctcct aattgctgga
    10801 tagatagagt tttaccgatg gacatcaatt caccgacaat aatgacaatg acagaaatca
    10861 aaaagattgt tttgaacagt cctaggtggt ttttcagcca ttgtatggta ttttttaaca
    10921 aataaatcac cttctattcc taatagttat gtttcagaaa gaaattgaag aacttctcga
    10981 ttgaagcgtt caggatcttt tctagctaat tcatgtcctt gttctttcac aagcacaaag
    11041 gaagcttttg gtatagtttt agctatatac aaagaatgtt ttagcttgat tacatctttt
    11101 tttcccacga tgatcaaagt gggggagttg atttttttta aatcattttc tgtaagcccg
    11161 atgtcatgca gcaaaagttt gataactaaa agatttttgc gaagacttgg gcgaaataaa
    11221 gagagtaacc atacccatgc ataatgaata ttactgatga ctctggcaga aaaacgaacg
    11281 cctttaacaa gcgtgtttcc agaatttaaa atcaatcgat gaactttttt agggaagctg
    11341 ctggcaaata ccagtgctag atttgctcca tcactaaatc caagaaaatc tgcctgatcg
    11401 attttttcca gcaacatgat cgtattcaga tcctcggcca ttaactgaaa attcaacata
    11461 gatgcttcgt ttgtcgaacg accatgtcct cggctatcta ctagatacac ggtatagtag
    11521 cgttcgagta cagggacctg ttctgaaaaa aagcggccgc tgccgtcatt gccatgcagt
    11581 aaaaagagag ggaagccttg accgctcttt tcatagtaaa tttcgctccc atctggcatt
    11641 tttgcatatt tcttttcttt tttcacataa tcacctactg tgtaaagtat aacgattata
    11701 tcagtcattg ggcaattgtt tttactgaaa aacaacgatc attttcgtaa gatgtcaaaa
    11761 aatacaggaa aattcatttt ttcttaaggc gtacacagaa atcaaggaag aaatagccaa
    11821 gaagataaag aaatagtaag ataaaaaaag atgtcaaaat aactaggtga tgaaatgaag
    11881 cgaaaacgat tatctattat ccgtattctt ttttacatga tcattctgac ggttattggt
    11941 gcggtgttgt ttttaggagg aagaacttat cgttcggtaa aacgagtgga aagctatcaa
    12001 atagaaataa caacagctgc aaaaaattat catatggaaa actacgagaa tttgatcaag
    12061 gcgataatat tgaccgaatc aaaaggaaaa gggacagatc tcatgcagag ctcggaaagt
    12121 ctgcatggag aagctgagat cattgataat ccagctgaca gtattgatca aggtgtcaaa
    12181 tatttggcgg aaatgatagt agaagcaaag aaacaaggat gcgatttatg gacagctgtc
    12241 caagcatata attttgggaa agattatatt gcctatgtaa aaaatcatgg cggagaaaac
    12301 acagtagaat tagcagaagc gtattctaaa gatgtgctga gcccactttt aggaaatgag
    12361 gaagggaaac aataccgata ttggcggatc cagtccttgc tttacaatgg cggctatctc
    12421 tatcataatg gaggtaacat gttttatcct gatattgtca aaatgaatga aaaaataata
    12481 gagacctatg aaaaagtctt taagtaaaat agcaggaaat gttcaatcat gaataaaatt
    12541 aaatgaaaga aggagtgcgc aaatagtgga aaaaaggcaa aaaatggaaa atcaagaagt
    12601 ggggtacaca accaaagact ggatgttacg gtttgtgaag ggaatgttta tcggttcagg
    12661 atttatcttg ccaggagtca gcggtggtgc attagcagct gtctttggca tgtatgagcg
    12721 gctgatttct tttttagccc accttacaaa aaatttcaaa gaaaatgttt tgttttttct
    12781 gcctgttggt ctaggcggag tgacaggcat ctttctactt tcttttttag tcagtttttt
    12841 attaggtgcg tatgaaacaa cgatattatg gttttttgtc ggatgtatcg ctggaactgt
    12901 tcctgcgtta tggaaagaag cagggaaaaa aggaagagat acttctgatg tgatcatcat
    12961 ggttgttact tttgttctgg cttatttgtt tttattatac ggcgctcaat tatttgacgg
    13021 gcaagtcgat cagaattttg gaacatggct gattgccggc ggattgattg gtttgggtat
    13081 gatcgtccca ggattgagcc cttctaattt tttggtgtat atgggattgt ataaagcgat
    13141 ggctgacgga tttaaagaag tacgacttga agtgattgtg ccgatcggaa ttggtgggat
    13201 tctttgtgtt cttggattgt caaaaatcat ggattacatt ttctcaaagg catatagcaa
    13261 gctattccat ttcattttag gtgtagtttt tgcttcaacg attatgatca ttcctaccga
    13321 ttacgcggga ttgggagttg ctggggtact agcttgtcct gttcttttta tagccggtac
    13381 agcactaggt tggtggatga gccgtttgga ggaaaaatat aagtaaagat agattgataa
    13441 taaaaacagc acacgggtga ttcaaacaaa aaggttggca tcttttttgt aaaatcattc
    13501 gtgtgttttt ctataaattt tataaaaaca agttgttttt aatcttggaa cacgctagac
    13561 ttaaacacgt gattattttt gaaagttggc ggaaaatgga gaaaaagaaa atgaatggac
    13621 ttacacagca agaggtagtt cagaaaatgg cttctggcca gcaaaatgac taccaggaag
    13681 atgcatcaaa aagtacaaaa caaatattca gcgataatat attgacgtta ttcaattttc
    13741 tgaactttgg tattgggatt tgtctgttat ttgtaggtgc gtattccaac atggcgtatt
    13801 tagcaattat tgtcgtcaat atcgttattg gaatttatca agaaatacat gcaagaaatc
    13861 ttgtacggaa attatcgatt gtttcccaat cagaagtact agtgatccgg gacggtaaag
    13921 aacaatcgat ttctactaaa gagctggtat taggagattt ggtaaaaatc ggagcaggag
    13981 aacagatacc agccgattta caggttatat ctggacgggc agaagcaaat gaagctcttt
    14041 tgacaggcga atctgatttg attgtcaaag aaactggagc tgaattgctt tctggaagct
    14101 ttttgacaag cggttctgtc atggctgaag ttatccgagt aggtgcagat aattatgctg
    14161 tccgcttgac tagcgaggca aagactcaca aagctattcg ttctgaactt gtagattcga
    14221 ttcgcaaagt atccaaattc actagtttcg tgattattcc tttggggatc atactatttt
    14281 tggaagcact gttcatccgc gatgcttcta tcaaaatttc tgttattgca tctgcagcag
    14341 ctttgttagg catgcttcca aagggtttgg tattgctaat aagtattgca ttaacgacag
    14401 gggtaaccaa actggcaaaa aaacgaatcc ttgttcaaga catgtattca attgaaacgc
    14461 tggcccatgt cgatacatta tgcttggata aaacagggac cattactgaa gggaaaatga
    14521 gtatccagcg ggtagaagca ctacatccgc tgtacgagaa agccatccca gaaattatgg
    14581 ggacgtatct ggcagaaagc agtgataaca acatcactat gcgggctatg agagattatt
    14641 ttgagctttc taatcggtat gatgtgacaa acaaagtcgc attttcttct gatcgcaaat
    14701 ggggcgcgat ggaatttcca gaactgggag tcgtgtacgt aggagcgcct gaacgtttgg
    14761 tggaagaaga tcggcttccg gacagtctaa aaacagccca agcgaatggc tatcgcgtac
    14821 ttatgttggg gatcgctcca aatcagcaat tgaacgaaga aaaaccagca tttgttgagc
    14881 cggttgctct gtttgaaatc gatgatccaa tccgaaaaaa cgctcaagaa acactagctt
    14941 atcttcatga ggaaggtgtg gatctgaaag tgatttctgg agacaatcct tttactgtct
    15001 ccaatatcgc tagacgtgca ggattaccga attatgatgc gtatgtcgat ctttctcaaa
    15061 ttactgaaga aatggaagtg cgagaagctg ctcatcgcta tacggttttt ggacgagtga
    15121 gtccgcagca aaagaaattg ctggtaaatg aattgaaaga aagcggcaga acggttgcaa
    15181 tgacaggcga cggtgtgaat gatgttttgg cgttaagaga agcagattgc agcattgcta
    15241 tggcagaagg tgatggagca acacgtcaga ttgcgaatct ggtattgttg gactctgatt
    15301 ttactacatt gccagaagtc ctgtttgaag gacgccgagt ggtcaacaat gtgacgaaag
    15361 tctcaggcat cttttttatc aaaacgattt attcatttat tttatcgatt atatgtgccg
    15421 ttacagcaat tggatttccg tttatcccta tccaaatcac gctgattgat ttagcgattg
    15481 aagggtatcc ttccttcttt ttatcttttg aacaagataa aagaaaaatc acttaccgtt
    15541 atttgcctac agcacttatt aatgctttac caaatgcctt gcttgtagta gtgaatatca
    15601 ttgcggtgta tctacttgga aaaacattcg catggggaaa tgctgaaact actactttga
    15661 tgtattactt gctgattggg atcagttgca tggcagttgt aaaagcttgt tatcccttca
    15721 acccattgcg catcttccta gcagtaacga cgatcgtagg gatatatgta gctgcaatgc
    15781 tgttccatgg aattttggaa gtaaacttag ggttgggaga gagcgtagga tacttctttg
    15841 tattcatgct cgtaaatatt gttcttcgag tattgtactg gaaggctgat attcaaaatc
    15901 agttactgaa aagagtagca aattgatgct taatgagata gaaatgtgtt atacttgaaa
    15961 aagcaatgaa atatgataca atcaaacatg tataattgtg tcctttaaaa tcgcactgat
    16021 ttcagttagt gcgatttttt accggttatt ctcctgaaat atcttctgtt aaacccaaaa
    16081 gatatcaata gagcaggggg tgaaaaaatg gctcaacgga ttcctcaaga ggttatcgaa
    16141 acagtacgca gtcaaacgaa tatcgttgag gtgattggac aatatgtcca gttgaaaaag
    16201 tctggtaaaa attacttggg gctgtgtcct tttcatgaag aacgtacacc gtctttttct
    16261 gttgcagaag acaagcagat ttttcattgc tttggttgtg gtaaaggcgg aaacgtgttt
    16321 acttttcttc aagaactaga aggtctaagt tttccagaag cagtggtaaa agtggcagac
    16381 ttcgaacaga taccgttaga agatcaatgg cgtcagcgta ctatagctgt ccaaaatcct
    16441 gaatctgcta cggggaaact gattgctgct catgaaaaag cagcggaaat ctatcatcac
    16501 atgctattgc atacaaaagc tggacagcca gctttagaat atttgcaaag tcgtgggttg
    16561 tctttagaat taattgaaga attcaacata ggttttgcac ctaatgagcg ttcttttctt
    16621 cagcaagtat tcaagaatga atcaatttca aatgaattgt tagaacgctc tggattgttt
    16681 gtcacgcatg atgatggccg tatgtctgat cgtttttatc aaagagtgat gtttccactt
    16741 cgtaattctc aaggccagac gatcggtttt tcaggacgct ggcttgcacc tgaaaaaggg
    16801 caggaaaaag atcagcccaa atacttgaat agtccagaaa cagaactgtt caataaaaga
    16861 caagttttat ttaatcttga taaagcaaga agcgatatcc ggaaaaacgg agaaattttg
    16921 ttgttcgaag ggtttatgga tgtgattgct gcatggcagg ctggtatcaa aactggatta
    16981 gcttctatgg ggacaagtct aacagctcaa cagatccaac aaatggaaaa gctgacgaaa
    17041 gaattggtct tttgctatga cggagacaac gcaggttttg aagcaaccaa tcgagggatt
    17101 gaattgcttc ggcaaaacag tcgattagca ctttctgtcg tagtcgttcc agaaaagctt
    17161 gatcctgatg aatatctccg taagtatgga gaagcatctt tccaagaatt agtggctcat
    17221 gggcgcgaaa cagtctttac ttttaaatca cgttatttaa aacaagggaa aaatttagat
    17281 aatgaaaaag acaagatcga atacctcgaa gaattactag ttgaacttag ccgagttgtt
    17341 tcaccaatcg aacaagatat gtatctatct caactgtcaa cagagtttca gatcggcaga
    17401 gagacgatac aaaaacaact gcgcgatctt cgaaggacaa atcagcaaaa tcgtcaagaa
    17461 cctcaggaaa gtcaacacac tcaggttacg agacaagaaa ctgcacggaa aaagagacct
    17521 ttgacgcaag tagaaaaggc agagcagatt ctattatatc gtgcatttaa agaagtttct
    17581 gtccgtaata ttttaaaaga acgagaattt cagtttattc atgatgcata tgcagaagtt
    17641 tattttttat tcgatgcata tgtctcacaa cattatgaat tcaaccttgc agagttttta
    17701 gactttttgc aagatgaaaa tttaaggcgg ttggtggtgg aaatcgaata tttaccggtg
    17761 gcagaagaaa gttcgccacg agaaatccaa gacttgctag atgtgatcag caaatctggc
    17821 ttagctgatg aaataacatt aaaaaaacaa atgcagcaag aagccagacg aactggaaac
    17881 aaacagcttg agcttgaatt gacgattgaa atcatcaatt taacgaaaca gttaaaacaa
    17941 gccaaatagc tactcaaggg gggccttctt ttatggcaaa tggaacagat aaaaaaacgt
    18001 atgaagcagc agtagcagct ttcatcaaag aaaacaaacc caaacgacaa gttgtatatg
    18061 atgatttatc caatcagctt gctacaccat tcactttgaa tgcagatgaa atggacaaat
    18121 tgatccaaaa gattgaagac gcaggtatca gtgtggtgga tgaaaatggt gatccatcaa
    18181 ttcacagctt gaagagtgct gagaaaaaag ctgaacaagc aaaaacagaa gatctatctg
    18241 cgccaaccgg tgtaaaaatc aatgacccgg ttcgtatgta tctgaaagaa attggccgtg
    18301 tctccctttt aactgcggaa gaagaagtag cgttagcatt gaaaatcgaa gaaggcgacc
    18361 aagaagcgaa acagcgtctg gcagaagcaa accttcgttt agtagtaagt atcgctaaac
    18421 gttatgtagg acgaggcatg cagttccttg acttgatcca agaaggaaac atgggactga
    18481 tgaaggcagt tgaaaaattt gactaccgca aaggatttaa gttttcaacc tatgctacat
    18541 ggtggattcg tcaagccatc actcgagcaa tcgctgacca agcacgtacg atccgtattc
    18601 cagttcatat ggttgaaaca attaataaat tgatacgtat ccaacgtcaa ttgctgcaag
    18661 accttggaag agaacctacg ccagaagaaa tcggtgcgga aatggatctt ccaacagaaa
    18721 aagtccgaga aatcttgaaa atcgcgcaag aacctgtttc ccttgaaacg ccaatcggtg
    18781 aagaagatga ctctcactta ggtgatttca tagaagacca ggaagcaaca agtccagcag
    18841 aacacgcagc ttatgagtta ctaaaagaac aattggaaga tgtactagat acattgactg
    18901 atcgggaaga gaatgtcctt cgcttacgtt ttggtttaga tgatggccgg acgagaacat
    18961 tggaagaagt agggaaagta tttggtgtta ctcgggaacg tattcgtcag atcgaggcta
    19021 aagcattaag aaaattgcgc catccttctc gttcaaaaca attaaaagat ttcttagaat
    19081 aagcgtggac tattgaccag aagagaaatc agtaaaactg tttggtttat tttaaaagaa
    19141 aaaaagcttg agactacgca gtctcaagct tttttttcta taggaaggaa attcattcat
    19201 tatttttaag taaatcctaa aaataagcta ttggcgtgac gtatagaata tgatagatta
    19261 aatggtagcg taaacctagt aaaggagccg atcgctaagt gatgaaaaaa tgtccaaatt
    19321 gcggatcgaa aaaaatggag cagaatacta tctgtccaga ctgcggtcat caaataactg
    19381 cagaagatca gcaaataaaa gaatcaattg aagaaacaaa tacagatgca gctatgcagg
    19441 ataaatcaac tgaagatgca gtctttcctg atacagaatt aaacgatcca atcgaatggt
    19501 cggaattgaa agatttacct ctagaatctg taatggaatt attcgaaaaa gcagaaccag
    19561 aagacgaaga accacctgct cacgatcaag taactaatat agagaaaaat gaacagaaga
    19621 aagcaactgt tcatgagact tcggaagaat caggaaagaa tcaagaagaa acgcttgaat
    19681 caaaacaaaa aagaagagta gctgaactga aagaaatcgt cgataatgag gaagaaaatt
    19741 cgatactatc cgcttatatc aaagcacatc gtgaagatac aaaagaagag cacgcgaaag
    19801 agctgctcaa aatgatcagt gaaaaaattg cacaggaaaa caaagtagag ccagaaaagg
    19861 aacaagaaac tgaaaaagac tctgatagag aaaacgccac aggtaaagaa acagtaaaaa
    19921 taaaagacgt aaaaacggat atcacagaaa aaactgaagt atttactgaa aaacaattag
    19981 aaaaggaaaa aacgaataaa ctgccggaag cagagaaaaa agaaatactc gaaaacacag
    20041 acaaaaagtc aacagaaccc gatgaaataa atagtgaatc cgatgctgaa aatcaatctg
    20101 taagcaaaga gggtactgat cttgaagcaa aaatagaaaa agaagagaac cagcctaagc
    20161 tgtttgaagc agaagcgaaa tcaataacaa tcgtgccaga tactgaaaaa acacatcaag
    20221 aatcaaactt agaaaaaaca gatccagata aagcgcaaaa gaaaaaatca aaaaaagtac
    20281 cttatctcgt tttagcagca gtacttctac tcggtggagg aagctgggct tattatgacc
    20341 atcatcaaaa agtagaagcc gaagtagcag cagaagcaaa aagacagcaa caaatggaag
    20401 aacttcatga gaaacttgct gccttttata cagatgatca ccatcaattt attcgtgcag
    20461 atatgattca tcaagattta tcaaaactaa aaaatgattt atccagtctt aagaatgaaa
    20521 aaggatattc agagttagaa acaacttacc aagatatcca atctaagatc cgagatatcc
    20581 aaaaagtgaa tgagtggttc atagcacctg ccatcgtcga tgatcatcta gcagatgaca
    20641 tcaagttgaa agcagataaa gagatccaag caatcgaaac ggataacaca gattttggaa
    20701 aattgattca acaagcacaa acagaagcta aaaatcaata tgctgaattg caaacagcta
    20761 aagaaaaaac agctatagtc tttaaagacg gaaaagtagt ggattcagca acacgcgacc
    20821 aatacaagac ggcaaaagaa gcagtagaaa aagtgaaaaa tacagatcta tctgctcagt
    20881 tgaatgaaca attgaaacaa gtagataaga tgctcactga taaagagaaa aaagaagaag
    20941 aggaaaagaa aaaagcagaa gaagcgaaaa aagcagctga agaacaagca aaacaagccg
    21001 ctcaagcagc agctgcagct caagccgctc aacaagctgc ggtcgcacaa gcagcacagc
    21061 aagaagcggc atcctcgacc ccttcctctt caaactcagc gaatcagcca atcaaatctg
    21121 ccagagcaag tgatattgcc gatgcatcta atccagcatg gacgtgggca ccaggtgtcc
    21181 aaagcaaagt gattgctaca tgtatccaac gtggatatat cgttgaagga ggttacagat
    21241 tagaaaaagc cagaatcgaa aatggggaag gctattataa tctatatgcc acttcaacaa
    21301 aatctgcatt gatgaaaggc attggagaaa gtgctttgcc attttacatc gtaaccatca
    21361 actgtaaaac tggatggttc ggaggaaatg ggagcaatta attttaggca aatgaaagag
    21421 gctgtgagcc tgccgtttaa caccgtttat tcgaaaaagg ggagtatgac acaactgttg
    21481 tcatactcct tttttcacct ttccaaacca aggctcagct attcaagtag ataacattat
    21541 gttatgatag caaagaaatg tattaagaaa acctttcact atagagggag aaaaaaatga
    21601 acgatttatt agcaactata tttacaggta ttattattga tgaaaatgaa aaaaactact
    21661 ttgtccaaaa aaacggacaa acctttggct taagcaaatc agaaggtgaa cacaaaatcg
    21721 gtgaagcagt tgaaggattc ggttaccaaa atcaaaaaca tgaaaatcgg atcactacac
    21781 aaattcctaa aagccgtatc ggtcattacg cttttggaat cgttactgct acacgaaaag
    21841 atttaggtgc gtttgtcgat atcggtcttc cagataaaga ctttgtcgta tctcttgatg
    21901 aattgccgac aatgacagag ctatggccta aaaaagggga tcgcttgatg atttccctta
    21961 gagttgatgc aaaagaccgt atttggggca gtttagcaga tgaaaaaata ttcaaatctt
    22021 taagcaaata tggaacagaa gaaatgaaaa acaaagatgt gataggaaca gcataccgct
    22081 tgaaattaac aggtacgtac gttttaacgg aagatttcta tcttgggttt atccatcctt
    22141 ctgaacgata taatgaacct cgtctaggcg agcagataaa aggtcgagtg attggtgtcc
    22201 gaccagacgg tgtactgaat ctttcattga agccaagagc atatgaagct atatcagatg
    22261 atgcacaaat gattttaacc attttagaac gttctgcaga tcagcaaatc aactttacag
    22321 ataaatcgga tccagaagaa atcatgaaag cttttggtat cagcaaagga gcatttaagc
    22381 gcgcattagg taatcttttg aaacaaggat tgattacaca agaaaacggt gtgacaaaac
    22441 tagtcaaaaa atctaattga gaatcccttg catgtttttc ttaactagct tataataatt
    22501 ttaattaaca tagtgttata tcttttttta tttgtaatta ttataaattg ttggggttga
    22561 gtaaatggtc atgaatgcta tttcggcgat gaaaaaaaca aaacagcagc tacatgaagc
    22621 ggggtttaag ctgacaccgc aaagagaagc tactctgctt gtgcttttgg aaaatgagaa
    22681 agagcattta tctgcagaag aaatcttctt tttagtgaaa aaaaagagtc cggaaattgg
    22741 acttgctact gtttatcgaa cccttgaaat cttgacagaa ttgaaaattg tcgataaagt
    22801 aagttttaat gatggattag cccgttatga tttgagaaaa gaaggcgcca aacatttcca
    22861 tcatcattta ctatgcttga actgcggcac gatcgaggaa gtagaagagg atcttttact
    22921 tgaagtggag caagtagtag aagaacgtta tcatttttta gtaaaagatc atcggctaac
    22981 gttccatgga atctgtcagg aatgctatga aaaagaaaaa agaaaccagt ccgtctgagt
    23041 gtctagacgg ctttttcttt gctcagatta tggaaaaaga ctatcagaat gaagcggtat
    23101 ctgatatgat agagaggcat gagggggatt attatgaaag agcaaatcaa cgattatctc
    23161 cactacttga atattgaacg gggattgtcc gccaatacac gacaaagcta tgaacgtgat
    23221 ttggaacagt acttgcattt tctacaggag cagaaaattg cttcatggca ggatgtcgat
    23281 cgctttttag tcatccgttt tctggagaag ttgcatgaag aaaaaaaagc gacagcaact
    23341 gttactcgga tgatctcaag tctcagaaga ttccatcagt ttttacggca agagcgtatc
    23401 actgatcatg atccgatgca acatatcgat acgccgaaga aagcacagaa attaccaagt
    23461 acattgactc tgacagaagt agaaaagctg attgaagcac ctgatacgac aaaaacgtta
    23521 gggataagag atcgtgcaat cttggaagtc atgtatgcta ctgggatgcg cgtcagtgaa
    23581 ttgattggat tgaaattggg agatcttcat ctttctcttg gactggtaca gacagttgga
    23641 aaaggagata aagagcggat cattccttta ggtgattatg cgatccaatg gctggaacgc
    23701 tacttagacg aagcacgtcc attacttgta aaggacgcat ctgaaatgca tgtatttgtc
    23761 aataatcatg ggaaaggtct ttctcggcaa ggaatctgga aaaatctgaa gcagttagta
    23821 cgagaggctg ggatttacaa agaagtaaca ccacatacgc tgcggcatag ctttgccaca
    23881 catctgctag aaaatggagc tgatttgcgg acagtacagg aattgttagg tcacgcggat
    23941 atctctacga ctcagattta tacacatatc acgaagaaaa gaatgacgga tgtttataag
    24001 cagcattttc caagggctta gtcataggaa ggtgagaaga tgttaacaca ttaccggaaa
    24061 gaaaaccgta aaattgctat gggactattg agtttccata agaaactgac aggtaaaact
    24121 tctttgttga aggagattga tacgtacgaa tctgaagacg attatgaact ttttttcttt
    24181 acgccagaag gttcaaccaa cattcaaggg attattggta tttactgggc aacgaacgat
    24241 cagctgatca tccatgacat ttctttgaat ccgtcttatc gcggtgagaa tatgggcttt
    24301 caaatgttag atgaattaca ggaaaagtac ccggatgtct ccatccgtgc aactgaaatc
    24361 acagagcctt acattggaaa atggctagca aagaaaaaga agaaagagtg aatgagttga
    24421 cggagatcaa tattaaatta gatgtattcg agggtcctct cgatctgctg cttcatctga
    24481 ttcagcaaat ggaaatcgat atatatgata taccgatcgc tacgatcacg gagcaatata
    24541 tgaattatat ccatacaatg aaaacactag agctagctgt tgcaggagaa tacttagtga
    24601 tggctgctac attgatggca atcaaaagca agacattgct tcctgtccaa gaattcgtca
    24661 cagacgatga agaattatgg gaagaagatc ctcgagaaga attggtcact cagcttttag
    24721 aataccgtaa atataaatat gcagctgaaa gactgacaga aaaagcagag gaacgaagtc
    24781 tttattacac aaaagaacct atgaacatcg atgggttagc ggatgctgaa aagccgttga
    24841 agcgaggaca agtcaaaaaa atcgatttga tccgtgccat gcagaaaatc ttagaacgta
    24901 agaaattttc tcaaaaagtg gaaaaaacga tcacgctcga tgacacaacg atcgaagaac
    24961 gaatggtatt tatcgaagag cgtataaaaa gaaatgcgca tggctgtact ttcgattctt
    25021 tttttgagtc accttctaaa agtgaagttg tcactacttt tatggctttg ctagaattga
    25081 tgaaaaaagg gcagatcatt gcgcaacaaa aagaaaacta tgaatcaatt atgctttatc
    25141 cagcagtagg agataccgaa catgacgaaa ttaagtgaac ttgaagccct tctttttatt
    25201 gttggcgagg aagggatcag taatgaagaa ttaagcggac tgttaaatct cagcgaagca
    25261 gaagtcacaa gtttagtgaa agaattaaaa gaatcttaca ctcaaaactc gcactcagct
    25321 ttgcatattt ttgaaacgga aagccattat gtattaacga ctaaaaaaga gctggctccc
    25381 ttacttaagc gatatgcgca agggacatca aacacattat ctcaagccgc cgtcgaaacc
    25441 ttagcaatca tcgcttataa acagccaatc acaagagctg aaatcgagca gattcgagga
    25501 gttcaggttt caggagcggt acagcgtcta gctgcccata aactgattga agaaaaaggt
    25561 agagtagaag gaccaggtcg gccgatatta tatggaacaa ctggctactt cttagattat
    25621 tttggtttga aatcattaga agaactgcct gatacacaag aaatggaaat ttcacttgaa
    25681 gaagacgtgt caccagatct attctttgat ggaatgaata aaggagaata attgtaatgg
    25741 agagattgca gaaagtaatt gcccacgctg gcatcacttc tcgtcgaaaa gcagaagaat
    25801 tcatccttgc aggacgtgta aaagtaaacg gacaagttgt aagggaatta gggataaaag
    25861 ttgggaaaca tgacactgtg gaagtggatg aagtcccaat ttatcaagaa gaatacggtt
    25921 actatttatt gtacaaacct aaaggagtta tttctgctgt tgcagatgac aaaggaagaa
    25981 aagttgttac ggatcttttg ccgatggtaa aagaacgtat ctatcctgta ggtcgactgg
    26041 attacgatac ttcggggatc ttgctactga caaatgatgg agattttgct cagcgcctga
    26101 cacatccaaa acatgaagtg gataaagttt acgttgcaaa ggtaaaaggg atcgctacaa
    26161 aatcgatgct tgcacctttg acaaaaggaa taaagatcga aggacgaaaa acatctccag
    26221 ctaaattcca aattttatca gtggatccta agacaaacca cagtatcgtg gaattgacga
    26281 tccacgaagg acgtaatcat caagtgaaaa atatgcttca agcagttggt cttcctgtgc
    26341 aaaaactaaa acgtgaaaaa tacggagatc tgactttaca aggcctacgt cctggggatt
    26401 accgtgaact gaacaaaaaa gaagtcagca gtttgctgaa ccactcaaaa tgaacactta
    26461 ctttttataa gtgttagatg ttgcgttttt tgaatagata tgtataatga atatgtaaac
    26521 aaaaattata taaggttcgt cttcaggggc agggtgaaat tcccgaccgg tggttatagt
    26581 ccacgaccta ttttgcaaaa aaatagctga atcggtgaaa ttccgatacc gacagtatag
    26641 tctggataaa gaagatagag cttatttgac gacgcttgat caaataactc taactctatt
    26701 ttccctggaa aatagagttt tttcatttgg agagaaacgt caaaaagttc gctgaagagg
    26761 agtcctaaca ggaggatttc aaatgaagaa cacccgtgta cagaaaatgg ttgcagtagc
    26821 gatgtttgct gcgatcgcat tagttttaca gtattttgct tttccgatca tgccggcttt
    26881 tagttttttg aagatcgatt ttagtgatat cccggtattg atcagcatgt tcttattcgg
    26941 tccagcggcc ggagttgcaa cagcgttctt gcgttcagta ttacatctgc tgactacagg
    27001 tttttctccg gataatttgg taggagacac tgctagtttc ttatcaacaa cgatctttac
    27061 attcccgatt tattatttct ttagtgtaaa gaaacagcat aaaggaagaa acaaattttt
    27121 aggtgtcatt acaggaacca tcgctctaac tatttttatg agtatcgcaa attatttcgt
    27181 tattacgcct ctttacttaa tgtttttagg gctgaacgca acacagatgc tgggcatgtc
    27241 tttagcaaac tatgtactga ttgggatcgt tcctttcaac ttgatcaaag gattcatcgt
    27301 gagtgctgtc tttttagtgc tgcatgcgca acttcttcca tggttaagtc gaaaacaaca
    27361 tcagttcgaa caacgtcata cagcagtcaa ataaatatca aaaagatcaa gaaatttctt
    27421 ggtctttttt tttggaaaaa aatacaagac atgcaataat gtaaacgttt tatttgtttg
    27481 ttttgagtat ttttttgatt tatgcataaa aatcaatcaa ttctttgcaa atgtgaaatt
    27541 cttctccttt taaaagacga aaatgtttgt ttataaaaaa aaggaaacgc tacaatgaaa
    27601 aagaagaaag ctttaaaaac ctgaaaaaag aggagggaat agggaaaaac gaactttttg
    27661 aacaaaagtt ttacaaaatg cgtgttatta tttttgttct gatactcttt ttgataaaat
    27721 aacattagag ccaaaattgt tgtctctaac ggaaaagaaa ggaagaagaa gatggaacaa
    27781 acaaaaaaga aaaaatttca aatgccttca gcgtatacgg tcttatttat tattattgca
    27841 gtaatcgctg tacttacttg gtttatacca gctgggcact atagtgtaga cgatgcaggc
    27901 aacatcgtcg ctggatcata tgaacgagta caacaaaatc ctcaagggct gtgggatatt
    27961 ttcatggcgc caatcaatgg gatgctaggt gtaccagcgg gggagctaac taaagaaaca
    28021 cctgctgcaa tcccaatctc tttcttcatt ttggttgttg gtggattttt aggtattatc
    28081 aacaaaacag gggcactaga tgccgggatt gcttcagttg tgaagaaatt caaaggaaaa
    28141 gaaaaaatgc ttattccagt attgatgctg ttatttgcat taggcggctc tacttacgga
    28201 atggcagaag aaacgattcc attctatgct ttactgatcc cagtcatgat ggctgtaggc
    28261 tttgatacag tagtagctgt agcaatcgta ttagtagggt ctcaagtagg ctgtctagct
    28321 tcaacggtaa atccttttgc aacaggcgta gcttcacaag ctgttgggat ttcgatgggt
    28381 gaaggtattg gtcttcgatt cttgatgttc gttattttgt tagctatttc tattgtatat
    28441 gtgtatcgtt atgcatcaaa gatcgaaaaa gatccaacaa aatcattagt atacaaccaa
    28501 cgtaaagaag atatgaagca tttcgatatt gaagcaatcg gtcgtcaaga agtaatcacg
    28561 aaaaaacaaa aacatgttaa tgtattgttc tttattactt ttggtatcat gatcatcagt
    28621 ttgattcctt ggacaacctt gaatgcgaat ttcacgattt ttgaatcatt gacaaactgg
    28681 ttaacaaacc ttccggtact aggtacggtt ctaggtaaaa gcatgcttcc attaggtgac
    28741 tggtacttcc cagaaattac tatgttattc ttagtaatgt ccatcgtagt agcaatcact
    28801 tatggcatga aagagtctga atttgtctct gaatttttag ctggtgcttc tgatttgatc
    28861 ggtgtagcaa tcgtagtagc tgttgctcgt ggtatccaag tggttatgaa caatggatta
    28921 atcacagaca ccatcttaca ttggggcgaa caaggattaa gttcattatc ttcaagtgta
    28981 ttcatcatta ttacatttat tttctatatc cctatgtcat tcttgattcc ttcaacttcc
    29041 ggcttagctg ctgcaacaat gggaatcatg ggacctatgg gtgaatttgc cggcgtcggt
    29101 aaggatttag taatcactgc ttatcaatct gcatctggtt tagtaaactt aattacacca
    29161 acatctgcta tcgtaatggg tgctgtagca attgctcgtt tcgatgtttc agtgtggtgg
    29221 aaatttaccg gtaaattaat tgcgatatta tttgtagcca tctgtgtgat cctaggtgta
    29281 gcagcaatgt tctaaaccta gacaaataca ggtacaaggt ctatcataag aatgaagaaa
    29341 ccgataaacg gctatcgata agcacgtgga caagtcaata gctccattca gtgagtgttt
    29401 tttctttcat catttgttaa gaacattctg cttgctgatg gagcaggctt aaaacgtcat
    29461 tatttgagaa gaagaggttg tgtcggagga atttgacatg acccttttta aaatattagg
    29521 aggataatat catgaaaaaa tttgtgacag aacaacatca tgaacaagca ttagaatcat
    29581 tgagcgaatt gatccgaatt ccttccgtac tagatgaagc agattcagga caaggacatc
    29641 ccttcggtaa aaaagtgatt gaagctttgg ataaagtatt ggaaatcagt gaaaagctag
    29701 gatttagaac atttaaagat ccagaaggct attacggtta ttcagagatt ggctctgggg
    29761 atgaattatt cggtatcttg tgtcatatgg acgttgttcc agcaggggac gaaaataatt
    29821 gggaaacaaa accattcgat ccaactataa aagacggctg gttagttggt cgcggatccc
    29881 aagatgacaa aggtccttct attgcggcaa tgtatgccgt caaagctttg attgatgctg
    29941 gcgtggaatt caagacacgt attcgtttta tctttggtac agatgaagaa aatttatggc
    30001 gttgtttaga aaaatataac gaaaaagaag aaggaattac gcaaggattt gcgccagatg
    30061 ctgaatttcc tttaatttat gcagaaaaag gcttattgca agcttacttg acaggaccag
    30121 gaacgaacga gttttctgta aaagctggag gagcgttgaa tgttgttcct gatgcagctc
    30181 cttactctgg agaaaagctt tctgaagtaa aagaagcatt gaaaaaacac gaattcgatt
    30241 tcgaagaaca aggtgaaggc atcgtcgttc ttggtaaaag tatccacgcg aaagatgcag
    30301 cacaaggagt gaatgctatt tctcgtctgg caattgctct ttcagaagta tttgattttg
    30361 gcccaatcaa tttcttagga aaattagttc aagaaaatgc aactggtgaa gctgtcgtgg
    30421 gcaaaacaga agatgaacaa tccggcgagc taacgatgaa ctttgcaagt ttggaaatca
    30481 caccagaaca aacaaaaatc ggtgtagata tgagaatccc tgttacattc aagaaagatg
    30541 atttagtagc taaattaact aaaacagcag aaaaatatgg gttgacttat gaagaatttg
    30601 acttccttga ttcattatac gtgccactag acagtgagtt agtgaaaaat ctattgggga
    30661 catatcgcga tattactgga gacatgactg aaccgtttgt atcaggcggc gctacttttg
    30721 ctcgtacgat gaaccagtgt gtggcattcg gtgcgatgtt ccctgatact cctgacttta
    30781 tgcaccaggc gaatgaacga tgggaattaa gtagtatgta taaagcaatg gaaatttatg
    30841 cagaagctgt gtatcgttta tgtgctaaat aaaaataaac atacaaaaag ggcggatatt
    30901 caaatggata ttccgtcttt ttttatttga tgctggaaag tttgcatttc tctcttattt
    30961 tttttaaggt ataatcatac ttgattaaga aaaaggggtc actcagatag aaaaggtggg
    31021 agaaagtgaa aatatttaga cgaatactaa ttggcttagt catcatttta ggattactcg
    31081 gtggagccgg ttattactat atacagcaaa atacgtacga accatcgtca gaagcgcaag
    31141 tgttggtaaa agaaggaaaa aacaaagaag attacttatt ttttgaagga gagaaaaaga
    31201 atcatggaaa gtctccatta cttatttttt atcctggtgc tctcgtagag ccagaaagtt
    31261 atagtatttg ggcgcaagat ttagcaaaaa acggcttttc agtcgctatt gtcaaaatgc
    31321 cacttgatct agcagtgatg ggcggaaaca aagcggataa ggtcaaagaa gattttccaa
    31381 attccgatta tgtgatcggt ggccattcat taggtggcgt aatggcaagt cgttatgtca
    31441 ataagaatca ttcagacaaa catttaaaag gggtattctt catggcaagt tatcccgaca
    31501 aaaaaggaac attgacagaa tcagctgttc ctgttttatc gatcacggcg acaaaagata
    31561 aagtattgaa tcaggaagct tatcaagaag ctaagcagta tttaccggaa agaacagagt
    31621 atgtatcaat caatggcgga aatcatggag gttttggcag ctatggtgag caaaaaggag
    31681 atggagaggc agaaatttct aacgcagacc agcaaactgt gatcgtcaat acattggaaa
    31741 actggctgaa aaagttgaaa taatataaat gggtcctata gggtccgtga aaaaactagt
    31801 caattaacta tagatagcaa atgaatcttt caaaataagt cgggatttca cggaacaaga
    31861 gaaacagatc ccggcttatt ttcaagttta gagaacttag tgtacgtttt taaagtttcg
    31921 tttatattta tattttattt tctaataaaa tcgctcgaac tggacatttt tgatacgctt
    31981 tttttacata tgtttcttct tccggcatca cgaactgttg atgagcctct ggttcttggg
    32041 caaataaaac gattccttct tcatcataat cgaaaatatc tggcgcttcg atttgacaaa
    32101 gaccgcatgc aatacagcgt tcaggtacaa gtttacatag tagtgacatt tctaaccatc
    32161 ctaacttaag gagaataata tgattgtttt tattttatcg ctattttcat caggacacaa
    32221 gctgcggatt agttctcttt accaactcct tgtgggaaaa cgaacgactt cagtacttat
    32281 ttatggattt acccacgaac ttttgtttat ccataacagt tttccagaat tgaagcagga
    32341 taagttttat caaattttgc aaaaaattgc gcagcaaggt tggatcgaaa taaatgaaaa
    32401 tgaagcaaag ctaacgcctg caggggctga tatgttgtcc gaacatcaaa tagaacatac
    32461 tggtttgcgt tttgaccgct atggcagaac aggggaaact agttggcggc tcattaaatt
    32521 tgctgttcaa gtaatttcta atctggcaac gggtattcaa gactatcttc cagcagagac
    32581 aagtcctttt tatactttcc aactaaaaaa atggcttagt gaaagccaat tgccacgtga
    32641 aattttgatt gatactgcgt atgagagttt ggtgcagctg ttttcggaga taccagaaga
    32701 agcagcagat tttttagcta accaattatc tggaaatgag cgaacagggc tgcttcctta
    32761 tcagctggca aaaaacaatg atgagtcagc ggtttatctg caacaaagca gatgtatcca
    32821 ccttttgtta gcacaaatcg aaaaacggcc agattcgctt tggcacgctt tgatcgattc
    32881 attattacag caaaatttca atcaaagcat gatgatcacc aagcagatgt ttatgaatgg
    32941 tcagacaatt gaccagatca tggcaatccg tcatttaaaa agaggaacag taacagatca
    33001 tcttattgag tgggccttgt tttttgatga ttttccatac gagtggattc tctctgcaga
    33061 aacaatcgaa cgtttggaac caaataagga ttccgtgcga gagtggcgct ttagcgaatg
    33121 gaatgtagat ggacaattag attatggaga atttcgtttg tatcaaatct atctattgag
    33181 aaaggaagcg atacagaatg ttaataaatg aattaaaaaa atattttgga tttgattcat
    33241 ttcgtcccgg ccaagaagaa attattcagg cgttgcttga tggagaagat actctcgcta
    33301 ttttgcctac aggaacggga aaaagccttt gctatcaatt gacggggtat ttaatggaag
    33361 gtctggtcat catcgtgtca ccgctgcttt ctctaatgga agatcaagtg acgcaattac
    33421 aaaaacgagg ggagaaacga gtagctgctt tcaatagttt actttcgcaa tctgaaagaa
    33481 gatacgtcct ctgtcatcta tcgcaatata aatttttgtt tttgagtccc gaaatgttga
    33541 ctaatccttc tcttttagag catctgaaaa aacaaaatat tgcgttatat gtagtagatg
    33601 aggctcattg cgtgtctcaa tggggagttg atttccgtcc agaatatcaa cagttaggaa
    33661 aaatccgaaa gcttttagga aacccggtca cactggcact aacggcgaca gcaacagatt
    33721 tagtagcaag agatatacgt catgtactat ttgatcgacc accaaaagag atcagacaat
    33781 cagtcaatcg gcaaaatatc tcgctttttg ttcggaaaac gcagcaaaaa gaacaagaac
    33841 ttgaacactt catggaaaga gcacaaggag cggcaatcat ttattgtgca acaaaaaaag
    33901 aagtcgaacg attgtatcat ttattcaggg aacgttttac agttggatac tatcacggtg
    33961 gtcttgatgc agctcaaaga agacaacttc aacagcagtt tgtaaaaaat cagctgcagt
    34021 ttctcattgc tacgaatgca tttggtatgg ggatcgataa atctgatatc cgttatgtgg
    34081 tccattatga tttgccagac agtctagaaa actatgtaca agaaatcggt cgagcaggca
    34141 gagatcagga agaaagtgca gcaattcttt tttatcaaag tggggatgaa cgtatccatt
    34201 atttctttaa tcaactttct agagaacagc gacaaagttt tgagctttat ttggaatatg
    34261 ccacagagca agctcccttt gatgagctgc aaaaaaaatg gatggagctg atccagcagt
    34321 ctgaaaaacc agaaaattgg gtggaacggc tcaaacgtca ggaaaaagaa aaagaattcc
    34381 gcctgcagca gatgcttcgc tatattaatg aagaaaactg ccgcagaaaa ttcatcttag
    34441 cttattttgg cgaaaaactc tccgaaaagc cgcagaattg ttgtgatatt gatggagcaa
    34501 aagcagaact tctgccaaag aggaagcttt tttctaagac tgaacaactt cattgggaaa
    34561 cgattttact aaatttattt aaaaaaaaca tggaacattg attttttagg acaagacact
    34621 ttttcctatg gtataataca cacgatagag tttaggaggt aaaacagtga gcagaaaaga
    34681 tagacatcaa tctacagaaa ataacgaaac acaagaacca tgggaacaac caatctacga
    34741 tacagatgat gaagtttcat caagaagttc tcagagacaa caaaagaaag gaaatacatt
    34801 atttctttcg ctatttttga ttttactggt cttatgtatc gctattccag caggtgctta
    34861 tttctggatc agaaatggat caagcaacaa tgcaacagca gcaacttctt ctgaaacttc
    34921 gacatcagta gtagaaagtt ctacaagtac aacaaaagag gagtcaactg agacttcaac
    34981 agtagaatct agtacaatcg cggaagaatc tactgtgtct caagatacag gtgtaacgtc
    35041 agaaagcagt attgcagcag agacaacacc gacttcctca agtgaggcaa cttatcaagc
    35101 aggggattca agcagcaatg cagcagaaac aacaccttct tcatcagctg cagccggttc
    35161 aacaacgact gttcaagcag gcgaaggtcc aaaacaagtg gctgagcgtg cagggatcac
    35221 cactgatcaa ttgtttgaat tgaatggctt agatccaaac aactttatgc tttaccctgg
    35281 tcaagaatta agaattaaat aataatagcc gcgctttgcg gctttttttc tgagaaaaag
    35341 gagttatatc atgacacaaa tcagtattgc aatcgacggg cctgcttcat ctgggaaaag
    35401 taccgttgcc aagatattag caaaagaatt gaattatatt tatacagata ccggtgccat
    35461 gtatcgcgca gtaacttatt tagctattac gaacaatgta tctttttcag atgaagaagg
    35521 gctagtttcg ctgatcaaaa agtatcctat ctctttttcg caacaagaag atggacagca
    35581 tgtattcata gcatcaaaag atgtgacttt agaaatccgt caaccagatg tcgcaaaagc
    35641 tgtttctgaa gtctctgcac atggaaaagt cagagaagaa ttagttgcta tccaacgaaa
    35701 aataggggaa cgcggtggcg ttgtcatgga cggaagagac atcgggacag ctgtattacc
    35761 gaatgcagaa gtgaaaatct ttttagtagc aagcgtaagt gagcgtgcag aaagacgttt
    35821 caaagagaac caagaaaaag gaattccgac tgatcttgaa acactgacga aagagataca
    35881 ggaaagagac cattatgatt caacaaggga cgtctctccg ctgaagcaag cagaggatgc
    35941 tgttcgtatc gatacgactg gcaaatcgat tccagaagtc gttgcagcaa taaaagctgt
    36001 tgttttgaaa aaaggttatc ttctttttta aattaagaaa aaaaatgaaa aacaattgtt
    36061 ttttcaagga aaacgaacga aatcgcttta ttttttttga ataatcaatt aaactaaaaa
    36121 cagtaccgta cttacggcag aatgttggtt aggaggacaa gtgttatgac agaatttgat
    36181 caaaatcaag agaacaacca gtcaatggaa gacgcaatga aaagcgtaca agaagtaaaa
    36241 gttggcgatg ttgttaaggg cgaagtttta gtcattgaag acaaacaagt gattgtcgga
    36301 atcgaagggg ctggagttga aggagtcgtt ccggcaaaag aattatcaac tttgccggta
    36361 gaagatatca gcgaagtagt aaaagtcggt gatgtattag atttagtcgt tatcagtacg
    36421 atcggcaaag ataaagaaaa cggcagctac ctactttcaa aacgccgttt agatgctaaa
    36481 aaagtttggg aagatatcga aagagatttc caagagggca aggtcatcga agcacctgtt
    36541 acaaatgttg tcaaaggcgg tttagtcgtt gacgtcggtg tacgtggttt tgtccctgca
    36601 tcaatggtgg aagatcattt tgttgcagac ttttcagact ataaaggcaa aacattgagc
    36661 tttaaaatca tcgaaatcga accatcagaa aatcgtttga tcttatcgca taaagcggta
    36721 gtggaaaacg aaagagaaga aaagaaaaaa gaaattcttt caactatcca tgagggagat
    36781 gttctcagtg gacgtgttgc acgtcttact gattttggcg catttgtcga cctaggcgga
    36841 attgacggtc tagtccatgt ctcagagatt tcacatagtc atgtagcaaa accaagtgat
    36901 gtattggctg tcaatgatga tgtaaatgtc aaagtgttgt ctatcaatcc tgaacaagaa
    36961 cgtgtttcct tgtcaatcaa agacacacag cctggacctt ggtcagaaat cgatacaaaa
    37021 gctccagctg gcacagtatt agacggaaca gttaaacgtt tgacaagctt cggtgccttt
    37081 gttgaagtgt tccctggagt agaagggcta gtccatatct ctcaaatttc acataaacat
    37141 attgcgacac cacatgaagt gcttcatgaa ggtgacgaag tgaaagtgaa agtattagag
    37201 atccatccag aagaacatcg tattgcattg agtatcaaag ctttagaaga aaaaccagct
    37261 tcagaagaag aaccaaaaaa tgttgagtct tatgaattac cagaagaaaa tactggattt
    37321 actatgggag acattttagg tgatgcgttg aaagaagaag tagaagaaaa agcaaccgat
    37381 gattctgaaa aataattgat tttgtatctg tgactgagat gaaagcagct gcttttgtct
    37441 cagtttttct tttgaaaagc gattagtatt tcaataagga ataaaaatac ttcgtttaat
    37501 tgacaatcta tgcttgaaag ttggcaatgc attcgttaaa ctaagtgaga ttggaaactt
    37561 gtgaggagga aataatatgg caaatccaac aattgcaatc gttggccgcc cgaacgtcgg
    37621 taagtctacg atttttaacc gcatcgctgg tgagcgtatc tcgattgtcg aagatactcc
    37681 tggagtaact cgtgatcgta tttatgcaaa aggtgaatgg ttaggtcgag aattcagcgt
    37741 gatcgacaca ggcggaattg atcttggcga tgaacctttt atggatcaga tcaagcatca
    37801 agcagaaatc gcgattgaag aagcagatgt aatcatttgt gtagtcagcg gacgcgaagg
    37861 cgtgacagac gcagatgaga tggttgcgaa aattttatac agaagtaata aaccagtgat
    37921 tttagcagtc aataaagtag ataatccaga aatgagaaac gatatctatg aattttattc
    37981 cttagggcta ggcgatcctt atcctatttc aggaagccat ggactaggga ttggtgatat
    38041 tttagatgaa gcagtgaagt atttctctga agagactgaa gaagaggacg acgacacgat
    38101 taagtttagt ttgattggtc gcccaaatgt tggtaaatcc tctttgatca atgcgatttt
    38161 aggagaagaa cgtgtaattg tttcggatat tgaaggaacg acccgagacg caatcgacac
    38221 ccacttcgtt tctgaaagtg gtcagaaatt cttaatgata gatacagctg gtatgcgtaa
    38281 acgcggaaaa gtatacgaaa atactgaaaa atacagcgtg atgcgtgcaa tgcgtgccat
    38341 tgaccgttct gatatcgttt tgatggtact gaatgctgaa gaagggattc gggaacaaga
    38401 caagcgagta gcgggttacg cccatgaagc tggtcgtgga attattatcg tcgtgaataa
    38461 atgggatctt gtaaagaaag aaaccaatac aatgcgtgat ttcgaacaag agattcgtga
    38521 tgaattccgg tatcttgatt acgctccaat cgtttttgtt tctgcagtga ctaaacaacg
    38581 gttggaacgt ctgcctgaaa tgatcgaaca agtaagtatg aaccagaatc ttcgtatctc
    38641 ttcggctgtg ttgaatgata tcattatgga tgctgtagca atcaatccaa caccaacaga
    38701 taaaggaaaa cgcctgaaga ttttttatgc tacacaagtc gctgtcaaac cacctacatt
    38761 tgtcgttttt gtcaatgaag aggaattgat gcatttttct tatgaacgtt tcttagaaaa
    38821 tcaaatcaga aaagcattta cttttgaagg gacgtccatc cgaattatcc ctcgtaggag
    38881 aaaataggca ttttaccata tcgacgtttt tttttcaaaa gaaaaagaca gtttcgtttg
    38941 ttacgtttct atgactaaaa aaagcatgtg aaaattggta aaaaccttgc tattaagcgg
    39001 ttcatatgat atcgtgataa cagaatattg aagttaatca atatttcttt ttaaacttct
    39061 ggaccactca gaaagtttta actaaatgat atttctatca tatctcttgt ggaggaggtg
    39121 aatctatcca tggcaaacaa agcagaatta atcgaaaaag ttgcttcagc tactgattta
    39181 actaaaaaag atgcaactgc agcagtagat gctgtatttt caactattca agatgcttta
    39241 gctaacggcg aaaaagttca attaatcggt tttggtaact ttgaagttcg cgaacgcgca
    39301 gctcgcaaag gtcgtaaccc acaaactggt gaagaaatcg aaattccagc tagcaaagta
    39361 cctgcattca aaccaggtaa agcattaaaa gacgctgtta aataagacag ttgaaaaagc
    39421 gcttgtggct ctagggctac aagtgctttt ctttttattt cctagcagaa aaggaataaa
    39481 taagagacag agattccgaa tccgtatgtc atacatagaa aacacatgta ttttatttaa
    39541 tttttattta tttccaagtt atgaggtgag ctttgctctt atcttttcta gaatttgctt
    39601 actaatggta aaataaaaat aagaagagga gggatgcgta tggaacttga ttatcaagta
    39661 gaagatgcgt tgacgaaagt gttttacgga gaaacgctag tcagtcagct aagagaactt
    39721 tccattgatc agccaatctt atttttaacg aatcagcgct attatgattt gtttgcagat
    39781 aagatcaacc cgttatttgc aaatcaagca aacaatgact ggtatatttg tgccaacact
    39841 caatgcaatc atttaacaga attaaagaac ctgcttgatt tcgtcaaacg ctatcccgaa
    39901 aatcaatcca tgtttatcat cggatttgga aatgaaggga tcatggatct ggcaggtttt
    39961 tttcaaaaac atacaaccct tgattcatca ctatggatga tccctgtatc gatccgttct
    40021 ctaagccgag cattaacacc gcagcggacg attttgcagc taccaaatcg tgtagtgctg
    40081 caaaccatca atctgccaga acgcatcgtt tatgatcaaa caatcagcga gcggcaggtg
    40141 gacggaaagc agattgactt tttgactttt atctgttgcg gaatgatttg tgatcatcgt
    40201 tttttgcaaa atctgtataa aaactatcca aataaaaagc aattaacgac tcggccgttt
    40261 gccgggatga tcgaagaaat gatctatttt tatcaattag aggcagaaaa cctgtcaaat
    40321 tatggtaaac tgtttgaaca agctttttat ctgaccgaaa atggccatct actttctgct
    40381 agtatgaaaa aatttttagg aatgatgttt catttcgtgt ggaatctcca agcatatcct
    40441 tttgatttcc aactaaaaaa cttttttgtg tggctggact tgcttggcta tccaatccac
    40501 tggctggaac agatttcaaa gatggagtat ttggaaaaag ttcttttttt ggcagaaaaa
    40561 agcaaaaaaa tacttgtttt agaaaaaata ggtataatag acggatacca acatccaaac
    40621 gaaaaagaat tattgagaac aatgacggta tttcaacaaa tcgtcagtga gataagaggg
    40681 aattgaaatg aatacaaata gcgaaaaaat gttgcaagct ttatcagatg aagacttgtc
    40741 acaagcacaa atctttttag aaaaagcatt aaaagaagac ccagcagata tattagctga
    40801 actaggcgaa gaattgctgg ctattggctt tttgatcgaa gcaaaacaga tttttgaaca
    40861 actgatcaac caatacccag aaaatgacgg actgaacatc ccgttagcag aaattgcaat
    40921 cgaagataat gagatcgact tagcttttga atacctggaa aaagtggata agcaaagtga
    40981 ttactatccg caaagcttat tggcgatagc tgatttgtac caagttatcg gaatccctga
    41041 agtgagtgaa gcaaaattga aagaagcagc tgggttgctt cctgatgaac cattgatcca
    41101 gtttgcgcta gctgaattgt atttttcagt tgatcgtttc aaagaggcag cagtggttta
    41161 tgaaacattg ttggcaagcg ggataaatga aatttcagga atctcgatgc aggaacgttt
    41221 aggacaatcc ttaagtatgc aaggagaatt cgaagcagct atcgtacctt tagaagaagc
    41281 actgaaagaa gaaaggacag atgatcgact gttccagcta gcttttacga atcttcaatt
    41341 gaaagaaaat cagcaggcaa tcaactatct ggaagaactt cgagaagtta atccgcatta
    41401 tcaatcgtta tatctatatc taggacaagc tcttcaagaa gaagaaatga ttgaagaagc
    41461 acaaacagtg ttagaagaag gaatcaaaga aaatccttac caagtcgaac tttatcacct
    41521 ggcctcggaa aatgctttcc gtctgcatga tagacagcga gcagaagaac ttttactgaa
    41581 agctttagaa gtaggagaca aacaagatga aacattactt actttaagca atctttactt
    41641 ggatgatgaa agatatgaag atgtgataca agtgatcaat cagatggaag aaactgctaa
    41701 tccttatgca gaatggaacc tagcccatgc ttataatgaa ttagaagact ttaccgttgc
    41761 agctgtacac tatgaacagg cttatcatga actatcacat gaaccagatt tcttgaaaga
    41821 gtatgccttt ttcttaagag aagaagggca gctgaaaaga gcgcaagaat tattgactta
    41881 ttatttaaca cttgaaccag gagatatgga ggcactgtca ttattagatg acctaacaga
    41941 gaggtgagaa aaatgctaat tgatgtacgg gaaaagaaga attttttaac gtggatggtc
    42001 aatcatgtat cttttagccg aagagaagtt ttttggatct tgaattatct tgccaatcat
    42061 gaagcgattt taagcaatgt ccactttgtt gaaggggcga attttgcgga tcgaggactg
    42121 cagatcagtg atctatctgt tgaaggggaa cccatgaaat tgtttttgca gggaaaagaa
    42181 tttacggaca cagaccagat ttttcatgaa atccgtttga attggaaaaa gcctttatat
    42241 gtggaatgta tattcgaaaa tgcttggcag acaagagaat atctatctat cttagaagac
    42301 aatccatttt cttcttggga ttcttcagtc agtgaagaag atactgaatt aattgaacga
    42361 tattttcaag aaaaagaaga agaggcaaaa ctaaaaatgc tttatgctca aatcgatcaa
    42421 gcactagaag atggagatag ggatgctttt ttgaaattat ctgatgaagt taaccgaaag
    42481 atccttttgc agacacaaaa gaaaagtaac cgggtaagtg aacataacaa ttagttaaaa
    42541 atagtatagt actaaaaaag acaaaggtca gctggatgtg gaatctttcc tcatagccta
    42601 tctttgtctt ttttcttata gttttttcag taatcttgtc tgtggcttca aataagtaaa
    42661 gccttgccct tccacttcat gcacattgat gattgaaagg aaagcttttt cgtcaatctc
    42721 gtggacgatc ctcttagctt cagttaattc actagagcta acgaccatat agatgatttt
    42781 ttttgaatta ccagagaatg cgccttctcc agaaaggaaa gtcacacctc tttgcagacc
    42841 ggtcatcagt agtggtgcaa tctcttcgct tttgttggag acgacaagta gaccttttgc
    42901 agaatatccg ccatctaaga tcgaatcaat cacacgactg aaaacatacg aaacaatcaa
    42961 cgtatacatc atacgtttca agtcgatata ggttaatgaa gcaagtaaga cgatgatatc
    43021 aaatgccaat agggaacgcc ccatagtgat gccgtaattt tgttctaaaa tccgagcgat
    43081 aatatcactg ccgcctgttg ttccacccat gcggtagacg atcccgcttc ctacacctgc
    43141 aactaaaccc gcaagtagag aaacgatcag tagatcatgc tgtaaattga tttctagtgg
    43201 aaccctttgc cagatccata aaaagaatga caaagaaagt gtacctagaa cagtataggc
    43261 caaggaacgt ttaccgagga tctttcctcc tagtaaaatc aatgggatgt tgattaataa
    43321 agtcgtataa gctggatcaa taccaaataa agctcttaag atcaaggtga tccctgagac
    43381 tcccccttct gctaaatgat tagcgatgtt caagtaaaca agaccaaatg cataaatact
    43441 tgttcctata atgataaaaa gaacttcttt tactgttttt cgtttgacca tatgaattcc
    43501 agctttcaat tatgtatttt cacagaattt ccatttagaa attccttcct aaatgtagca
    43561 tggaaattcg attttctcaa ttgctcttat ctaggatttg cgttaaagtt ttggtaagat
    43621 agttttggag ggaaaaagat ggataaaaag cgttctttat acagtatgca gcaggaagtg
    43681 gatgaatata tccagcaatt caaggtgggg tatttctctc cattagctca gatggctcga
    43741 ttaactgaag aagtaggtga attggcaaga gaagtcaacc acacttatgg agaaaaaagc
    43801 aaaaaggctt ctgaaccagt taactcggta gcagaagaac ttggtgatgt tttattcgtt
    43861 acgatgatca tggctaattc attgaatatc gacttaactg aagtttttga aaaaaacatg
    43921 gaaaaattca atcaaagaga ccataatcgc tttgagcgta aggacgagta aacaagatga
    43981 agtggtcctg gccttgaaga ctctgaaacg gacttgtcaa acagtcacca tgcaactgag
    44041 tctttatctc aggacaagaa acacgcagga aaggttggaa aaatgaaatt aaaacaatta
    44101 cctctggaat accaaaaggc aattccagtt ctgaaaaaat tagagaatgc aggatatgaa
    44161 gcttattttg taggtggaag tgttcgtgat atcttgctga accagccaat ccatgatgtg
    44221 gatatcgcaa caagcgcatt tccagaagaa atcaaacagc tttttccaaa aacgatagat
    44281 attgggatcg aacatggaac tgtgttggta ttagaaaatg atgaacaata tgagatcacc
    44341 acatttcgga cggaatcaac ttatcaggac taccgtagac cagatcatgt cgaattcgtt
    44401 cgttcgttgg cagaagattt gaaaagaaga gattttacga tcaatgcgtt tgctttgaaa
    44461 gaagatggag aaatcatcga tttgttcgat ggtctgcaag atctagaaga acagatcttg
    44521 cgagctgtag gcaatcctca tgagcggttc catgaagatg ccttacgaat gatgcgcggg
    44581 ttgcgttttg tcagtcaact agggttcaaa ttagaggaag aaaccttcta cgccatcaaa
    44641 gaaaaccacg agctattagg taaaatatct gtagaacgta taaatgtgga attcatcaag
    44701 atgctcttgg gaaatgaacg ggccgcggga atccatgcat ttattgaaac agagtgctac
    44761 atttgctgtc ccggtttgaa agaagaaggc gaagcacttt tacgttttgc tgaattacca
    44821 tccgtcccat tgaaagaaga gagccaagca tgggcattat tgatcagcca actggatcta
    44881 aaagaaaatg agattcgttc ctttttgaaa tcgtggaagt gttctaacca aatgattaaa
    44941 gacgttcaag cacttgtcca aggactacga caacgagaaa ctggagcttt ggaaccattc
    45001 gaattgtact tcttaggcaa aaaacatgca ctgcaagtag aagaattgat gatctatttt
    45061 gacgaaacac ctcagatgga tgccgtagga gaatcctatg atcgtttacc tatcaagtca
    45121 ttaagcgaat tagcagtaga tggtaaaatc ttgctagaag aaactggaaa aaaacctggt
    45181 aagtgggtta gtgaagcttt gcaattagct gaaaaagccg tcgtagaaca aagaataaaa
    45241 aatgataaaa aacaggtttt ggatttttta tcgaaagaaa aattgattta aaaaacacta
    45301 gactgtatct gctcagcttt gttggctact taaagtgggc agatacgtca ttagaagaaa
    45361 aaacataaga tgtgaccact tacataaatt tgtgctaaac tgaggtagta caagaataaa
    45421 ttacatcata aaggagtctt tttaatgagt aaagaaatga cagcgctaaa attttatttt
    45481 agaaatggag aaacctggac gatcaaccgc cgtcatatcg gcgatctttg gatcaaacaa
    45541 atcacaacaa gttttggacg aatcaatggc agtgaatttg ttgaaatcca tccgtgtgct
    45601 ggatttaaaa tcgaaatctt ccatgaaggg gacgcagttg cgacacatga tatcaaccta
    45661 ggcggactgg aaatgggcat gtttaatcgc gcattgaaat acgaagatat cgagcgaatg
    45721 gaaatccttt accgaaatgg cacacctgat cttgtgtact tcccatatct agataaagga
    45781 acagaaggat tagacaatca atatcaatca acaaaaatca gcgaaaaaac aggtaacttg
    45841 tatatcgtga tcaatccaga tcaacgtgta gaagatgtat acggggaatt tttcgaataa
    45901 atcgattttt acgcaaaaat gaggctgtga aagagatgtg tcttattact actgataggc
    45961 tgtctcttcc acaccgttta ttcagaataa gggactgtga caagattttt gtcgcagccc
    46021 catttttttg ttgtgataaa agaacaagaa aggcaggaat gggaaagtaa acatcctttt
    46081 gtcagaaact tttatctttc ttaagtttta cttgatatct gatactattt ggaatagaat
    46141 gaggcgttga aaaatttatg gaaatttcta actagcaata aacgatgaaa caatgaaata
    46201 tttttcatcc aaaaagaact ttgctataat gatggaagtt tgtgtatagg agatctggaa
    46261 aatgagagag agttttgaac aacaaaaaaa actgttgcat gatcgctatg gcgccctttc
    46321 gatggatgac cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg
    46381 tcagttggaa cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg tacagtgtga
    46441 attagagggg aaccaaacac aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga
    46501 acaattttta aaaatgctta cacaaaataa aaaataaagg ggatggacga tggaatttgc
    46561 tgagttagtt attgtatttg cagtaacgat caccttatcg aacatagcta gccgcatttt
    46621 gccaatgatt ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga
    46681 gtggggacaa tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt
    46741 gttatttaga gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat
    46801 tttgttcttg gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc
    46861 attcctgttg ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc
    46921 gacagatgcg gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat
    46981 gcatattttg gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt
    47041 tgctcttggt gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt
    47101 agtttctagt attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat
    47161 aattcatttg atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact
    47221 tttgcttcct tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc
    47281 tgcagtggca gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc
    47341 agaattgtcg agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt
    47401 agtatttata tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg
    47461 tttatatcca aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt
    47521 gatcagattc atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa
    47581 aaaacagttg aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt
    47641 ggctacgatt tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt
    47701 gcttttgttt ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt
    47761 gcctatgctg acggaaacag aggaagcgga aagcacggat ttgaatgctt tgatgatttt
    47821 agaagaggtc gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga
    47881 atttttagcc actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt
    47941 ggaagattta accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc
    48001 gatcgaacgg gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta
    48061 tcgtttttac tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc
    48121 ttttatcggc ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa
    48181 aatgttttgg caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca
    48241 agaaatcaga gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt
    48301 gactgatatt tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg
    48361 aataaaaatg aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt
    48421 tacgaaaaaa atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt
    48481 tgctgaaaaa atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga
    48541 atcttatact atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa
    48601 gtcaaaaaaa acgagtccaa taaaataaaa aggtaaaaat tttcaagttt tataaaaaac
    48661 aaaacatatc agaacaggat cgaatcggtc ctgttctttt tttgagaaca caaaaagaac
    48721 aaataccaaa aaaattttcc tgaagttcac tatttttatg atacaatatc caagataagg
    48781 agggaacatg ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg gtgaaaaaga
    48841 tttgtttgac cagatttctt ttttggtcca cgaaaaagac cgtatcgggt tgattgggac
    48901 gaacgggacg ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata gcagcgacgg
    48961 tgatcggcaa acagtgtttt atccaactga ttaccgtatt ggttatcttt ctcaagcagc
    49021 tgaattttcc gatgaattaa ctgttctcca agcagtcttt caaggaaaca gccccttgat
    49081 ccaaactgtt cgagcatatg aggaagcatt gatcgaacta ggggaaaacg gagaagaccc
    49141 tgatgtgcaa aaacgttatg ctaaagcaga agaacaaatg aataaagaag atgcttggac
    49201 tacagatacc aacgcaaaga ttattttgca aaaattagga atcagtgaat tagataaaaa
    49261 aatcagtact ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag tgttgattga
    49321 tgaacccgat ttactattac tagacgaacc aacaaaccat ttagattatg aagcgatcga
    49381 gtggttggaa aactatttga aacaataccg tggcgcacta ttgatggtta cccacgatcg
    49441 ttattttcta gatagagtag caaaccgaat cttcgaactt tcatttggaa aattatacga
    49501 gtataaagga aattatgaag cttacgtctt agaaaaagca gaacgtgatc gtgtagaagt
    49561 agaacaagaa gaaaagcgca agcggcttta taaacaggaa ttagcttgga tgagggctgg
    49621 cgtacaggca cgtggcacca agcagcaagc acggatcaac cgtttcgaag atctaaagga
    49681 gaacttgtac caagtaaacc aagaagatga tttagagctg aacctagcga cacaacgttt
    49741 agggaaaaaa gtactggaaa tcaaagatgg cagttaccgt ataaatgacc aaacactgct
    49801 agaacattta gatttactga ttcaatctcg ggaaagacta ggtattactg gtaaaaatgg
    49861 tgcgggaaaa tcgacattgc ttaatattct agctggcaga attcccctgg atagcggtac
    49921 aatgtctatc ggtgaaacgg ttcatcttgc gtattataca caagaaaatg aagaaatggc
    49981 accagataaa cggatgattg cttacttaca agaagctgct gaagaagcaa aaacagcaga
    50041 tggcagtcaa atcggagtag cagaattact tgaacgattt ttatttccaa gatttatgca
    50101 tgggacactg ataggcaaac tttcaggcgg agaaaaacgc cggttatttt tgttgaaact
    50161 gctgattcag caacctaatg ttttattatt ggatgaaccg accaatgatt tggacatagc
    50221 aacattgacg attttggaag attattttag aagttttcca ggtgctgtga tcactgtttc
    50281 ccatgaccgt tatttcttgg acaaggtagc agataagtta ttagtattcc aaggaaacgg
    50341 aaaacaagaa ctttattacg gaaacatgtc cagttattta ctgaagcaaa aagagaccca
    50401 gcagcctgct gaaaaagcga aaccaaaaat acagagcaag gaacctgctg aaaagaagaa
    50461 attatcttac atggaacaaa aagaatggga aacgattgaa gatgagattg cagaacttga
    50521 agaaaaaatc tcattgctcc aagaagagat gaatcatcaa ggagataatt tcactcgatt
    50581 acaagaattg caaaacgacg tatctgaaac agaagcacaa cttgaagaaa aaatggcacg
    50641 gtgggaatac ttgagtgaat gggtagaaga ttaggaggaa aaaggatgga acaagcgtat
    50701 cttgacttag gaagaaaatt gttggaagaa gggcatcgta aagaagacag aacaggcaca
    50761 ggcacattaa gcttattcgg ataccaaatg cggtttgatc tgcaaaaagg gtttcctttg
    50821 ttaacaacga aacgagtacc ttttggattg atcaaaagcg aattgctttg gtttttaaaa
    50881 ggagatacga atatccgcta tctattacaa aataaaaacc atatttggga tgaatgggca
    50941 tttgaacgct acatcaaaag tccagactat aatggaccag atatgactga ttttggccat
    51001 agatgtttga cagatcctga attcaatgaa gaatataaaa agcagagcaa gcttttttgc
    51061 gagaagatcc taaccgatga ttcctttgct gaaacatacg gcgatcttgg acacatctac
    51121 ggctatcaat ggcgtcactg ggaaacaaaa gacggtggct ttatcgatca aatcaaacaa
    51181 gtgatcgaag caatcaaaaa gactcctgat tctagaagat tgatcgtttc tgcatggaat
    51241 ccggaagatg ttcctagcat ggctttgcct ccttgtcata cgatgtttca attttatgtt
    51301 caagagggac ggctaagctg ccaattgtat caacgcagtg gggatgtgtt tctaggcgtt
    51361 ccgttcaaca ttgcaagtta tgcgttgctg actcacctaa ttgcacatga aacaggttta
    51421 gcggttggcg aatttgttca cacattagga gatgctcatc tttatatcaa tcatatcgaa
    51481 caaatgaaag aacaattgag ccgagaaata cgcgcatttc cgactttagt tttgaatcag
    51541 gaaaagcagt cagtatttga ttttgagatg gaagatatcc aattagaagg atatgaccca
    51601 catccggcga tcaaagctcc tatcgctgtt tagaatcaga aaggatgaga aaatgttagc
    51661 tgcaatttgg gcacaggatg aaaacggatt gattggaaaa gaagaccgtc ttccttggcg
    51721 tttgccaaat gatctgaaat tcttcaaaca aatgacagaa gcaaatacac tagtgatggg
    51781 acgaaaaaca ttcgaaggga tgggtagccg tccgttgccg aatcgtcaga cgattgtttt
    51841 gacgagagat tcttcctata aagcagaagg cgtgcatgtc atgcatagtg tggaagacgt
    51901 gcttgctctg gaaaaagaaa cagacggtat ctttttcatc gctggagggt cagctgttta
    51961 tcaagaattt ctgccatttt gtacgatatt ataccgtaca gtcatccatc atgtattcga
    52021 tggagatgct tattttccac ctgttgactg gtctgattgg tcactgatca atactagtca
    52081 aggagaaatg gatgaaaaaa atcgttatcc gcatcaattt gaaacgtatc agcgtaatga
    52141 gaatcctgta aaaagcggga caggcgaatt gacgatataa aaaagctatg gatagactag
    52201 ctgttattta gagctgtccc atccatagct ttattttttt tattaagtaa agaataaaac
    52261 agagaagtac ataaaacctg cacctaacat aacaaacaaa tgccaaacaa catgcatata
    52321 gcggatagaa ttcaagctat aaaagaatgc gcctatcgtg taagaaagcc cgcctgctac
    52381 catcaaaata acaccagtca ttccaagaga gtgataaaga ggaacagcag ccacgacaca
    52441 gagccacccc ataaaaatat agatgatcgt agatactttt gatactctct cccgtttatg
    52501 caacgtcaac gctttatata taatgccaag aatcgcaaac aaccagatta atacaaataa
    52561 agtccaacct agccatcctc ggatactcag caaacaaaaa ggagtataac ttcctgcaat
    52621 caaaagaaaa atcgaactat gatcgaatac ttggaacacc ttttttgctt ttgtaaaaat
    52681 caatgaatga aacaaagtag acgttaaaaa taaaagaatc atcatcgatc catagatagc
    52741 ataagagaca atatggaggg aggaatgcaa gtgggcacct tttactaaaa gaaccactag
    52801 tcccgcgata ctcaatccag tcccaattcc atgggtgact gcattaaaca cttcatttaa
    52861 tatttcatac gttcgattaa atttcgtttt ttccatgaaa acggctcctt tactaggtgt
    52921 aattaatgaa aaatcattat ttgtctgtta tactaaaaat agaaattgtc ttacatgtag
    52981 tttaacatga ataaggcatc ttgttaaaga aagagagaga gaaaataatg acaaatgtaa
    53041 aaatcgtaac agattcttca tgtacgatgg aaaaaaatgt acgcgatgct ttaggcattc
    53101 gtgtcatgcc gctatccatc atgatcgacg gaacagtata tcccgatgat gaccaactag
    53161 acggcgaaag atttatggaa atgatggcga aagcaaaaaa tctgcccaaa acaagccaac
    53221 caccaattgg agaattcatc tcgatgtatg atgagctagg agaagacggc agtgaagtga
    53281 tttcgatcca tatgacaaaa ggcttgagtg gaacagtaga agcagcaaga caagccagta
    53341 atctaacaaa aacgaaagta acggttatcg acagtgatac cactgaccaa ggattatctt
    53401 ttcaggtaat tcgagcagct gaattagcaa aagaaggtaa aacagtagag aaaatccttg
    53461 aagaagtcaa aaaagtacaa gacaatacaa aattatacat cggaatatcc acactagata
    53521 acttggtaaa aggtggaaga atcagccgaa caactggttt gctgtctagt gttttgaaca
    53581 tacgcgtggt gatgaatttc gcccattctg aattaatccc tgtaactaag gggcgaggaa
    53641 agaaaacgtt cgataaatgg tttaataatt tgaaaaatga actgaaagaa ttgccaaatg
    53701 tgcgacaaat cggtatttca catgcggatg caaaagaatt ggcgcaagaa ttcaaagagg
    53761 gtttgcaaga attattcccg gacatgcata tccctgtact gcatacgaat ccggttatcg
    53821 ctacgcatac tggtaaagac gcgtttgcaa tcatgtatta tgtagattag tcgaaaagag
    53881 gataagccct cagcctgaaa ggcttgcagc ttattctctt tttctttata aaattaggaa
    53941 aataattgtt ttcttttttc ataaagacta cctttttgat aaaatttttg ataacattag
    54001 aaccaatcga atacaagagg tgaaaaaatg aaattaacga aacgacattt gatcctcgtt
    54061 ttgttcccat tattaattgc gatcatcatc tatgcaggtt tagctgcttc tataccgaaa
    54121 gctggcccac taatgaaacc agtaactact gtgagagaag atccaaataa aaaagaaaaa
    54181 cttcactaca tagctatcgg agattcgctg actgaaggag taggagatca aaccaagcaa
    54241 ggaggattcg ttccccttgt cgcaaatgat ctaaaagacc gttataattt gaccgcggtt
    54301 gaaacagaaa actatggtgt aaatggtgaa cgaagtgacc agatcttaaa gcgagtaaaa
    54361 acaaaagaag aaattcgaaa caatatcaaa acagctgatt ttatcacact gactgttggc
    54421 ggtaatgatt tgatgaaagt tatccaaaat aatttattcg ggttaacggt caattccttt
    54481 aaaaagccac tgaaaaacta tcaggaaaat gtcacagaac tattggaaga atttcgatcc
    54541 ctcaataaaa ccgcacctat ttacatttta ggcatctaca atccatttta tttgaacttt
    54601 cctgagatca ctgacatgca gacaattgtt aacaattgga acaaaggaac agaaacggtg
    54661 acaaaaaaaa tgccgaactg ctactttatc ccaatcaatg atttgcttta tcaaggattg
    54721 aatcaggaag taggtgtcac agaagaagga acagatcaaa caactagcac gacagaaaat
    54781 ctaaatggca tcaaaaataa tgtgctctat gaagaagatc atttccatcc aaataattta
    54841 ggttatcaat tgatggcaaa tgcagtgaga gatcagctga tcgagacaca aaaccaatgg
    54901 ttagagaagg aggatgatca ataaacatga aaacaagatc tgacgtatct tcaaacaaaa
    54961 caaagaaaac aaattactgg aaattcgcct tccttatcct tcttggggta gtacttggtt
    55021 cgaccgtatt tttaggcagc cgtatttttg ctaatcggga accagaactc ccagaaatac
    55081 cagctcttac ggaaagacaa ggagatcctg tcttaacgat caattcaaat aaagaaaaag
    55141 tcaatcaaat catcagcttt ttccttagtg agtatcaaaa agatagtgat attgaatata
    55201 aattttattt ggaaaatgaa gcactactca atggtacttt cgaagtacta ggttttccta
    55261 tcgatttcta tttatatttt gatccgtatg tgatggaaaa cggaaatgtc caactaaaag
    55321 ctagaagtct ttctatcgga acattgagtt tgccaatcaa agatgtgatg aatatgatca
    55381 agagaaacta taaattacca gattggatcg aagtcaatac agatgatctg actatcatgc
    55441 ttaggttgga tcaattccga atgcaaaacg ggatgtatat taaagcagat aaaatcgact
    55501 tagtaaatga tgacattcgg tttagtctat atttgccaaa agatacctct gatgcttcaa
    55561 acaactcaaa taccaacaaa actagcacca gcagtactga aagcaagaca aaagaaaata
    55621 aaaaatcatc ggatgagtag tattagaggg ggaatcataa tggaacgagc aatatttgca
    55681 ggaggctgct tctggtgtat gatccagcct ttcgacacgc ttcctggtat ccatacagtg
    55741 atgtcaggat atactggagg gcatgtacca gatccgactt atgaacaagt aaaaagccaa
    55801 acgactggac atacagaagc agtggagatt atctatgatc caacattgat ttcatatgaa
    55861 caattagtag aactttactg gcagcaaact gatccaactg acgctttcgg acaattcgag
    55921 gatcgcggtg acaattatcg cccggtgatc ttttatttga cagaagaaca gcggatcatt
    55981 gctgaaaaaa gtaagaaaca gttagctgaa agtggacgtt tcgaacagcc aatcgttaca
    56041 aaaatcgaac cagcccaacc attttatcta gcagaagaag accatcaaga gtattataaa
    56101 aagcatcctg aaaattttaa aagaaaccat gcacgccggg ctgcctttat cgaagaaaat
    56161 tggaggaacg aagggtgaat cgaagctttt ttcattattt aatgacactg cgaggcggtg
    56221 gcacacatga tgctttatct gttttcgcta cagaagccgg taaagacatc caatttccta
    56281 agcacagctc tagctacgaa gaaatttcta attatctgga actgaatgtc gactatttgc
    56341 cgtccatgga tatctttgac gaagcatggg aaaaatattt agaaaataat catgacatat
    56401 aagagatagt tttgtacttt atgatcaaaa aagttttttg tccaatagcc taattgaaag
    56461 aattattttt atttcagcta tctagtcgca tttttttgat atttttctta taataaagaa
    56521 agaatgaaaa aaagagggat ggattgatag agattgacaa aaaaacaacg ctatattaat
    56581 cgcttctggc ttgtattcaa atacttactt gtttctgtat tccttgcgtt catgttgcgg
    56641 ggatttttat tcatcccggt accagtggaa ggcaattcaa tggaaaacgt gttgaagcaa
    56701 ggtgatatgg ttgtgatgga aaaattttcg gagatcagac gatttgatat tgttgttttt
    56761 caattagcag atggcacgat ttatatcaaa cgagtaatcg gtcttccagg agaaaatgtc
    56821 agctaccaaa atgatcaatt gaagatcaat gggaaagtag ttaaggaacc ttatctaact
    56881 aaaaacctga aatcagatca tgcaaatgca tcctatacga cggactttac actacaagag
    56941 ctaacgggac aatccaagct tccggaagat aattattttg tacttggaga taatcgaaga
    57001 gtttcaaaag atagtcgttc gtttggcacg atcaataaaa ctgatattct aggtaaagca
    57061 agatttgtct attatccact tgatgaaata aaatggatcc agtgagaaac aataaagcgt
    57121 gcagtctgtt gagactgcac gctttattgt ttaatccaaa atcagtttac taccttagta
    57181 cgtaaaatca aacagctcgt ttaaacactt ttctagcctc taaagttttg ataaatgaat
    57241 aaacaaccat tatttgaata gggatttgga tcatattcct gatgaatcta cctagaaaaa
    57301 ttactttgaa atctgcacca aacaaaacag acatcagcca agaattgata aaaaggctga
    57361 ctgggatcat aatcaataat tgggcagcga tgacggaagg caggtttatc tttttatg
//
LOCUS       DAEGIQ010000005        53988 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000005 DAEGIQ010000000
VERSION     DAEGIQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.005"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(67..1488)
                     /locus_tag="KMT45_000261"
     CDS             complement(67..1488)
                     /locus_tag="KMT45_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7003752.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1731..3608)
                     /gene="tdc"
                     /locus_tag="KMT45_000262"
     CDS             complement(1731..3608)
                     /gene="tdc"
                     /locus_tag="KMT45_000262"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBE7003753.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3899..5155)
                     /locus_tag="KMT45_000263"
     CDS             complement(3899..5155)
                     /locus_tag="KMT45_000263"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBE7003754.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5267..5472)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5735..7036
                     /locus_tag="KMT45_000264"
     CDS             5735..7036
                     /locus_tag="KMT45_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBE7003755.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7105..7704)
                     /locus_tag="KMT45_000265"
     CDS             complement(7105..7704)
                     /locus_tag="KMT45_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE7003756.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7958..8839
                     /locus_tag="KMT45_000266"
     CDS             7958..8839
                     /locus_tag="KMT45_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7003757.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8852..10462
                     /locus_tag="KMT45_000267"
     CDS             8852..10462
                     /locus_tag="KMT45_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBE7003758.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10539..11198)
                     /locus_tag="KMT45_000268"
     CDS             complement(10539..11198)
                     /locus_tag="KMT45_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBE7003759.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11481..12146)
                     /locus_tag="KMT45_000269"
     CDS             complement(11481..12146)
                     /locus_tag="KMT45_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBE7003760.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(12293..12898)
                     /gene="dhaL"
                     /locus_tag="KMT45_000270"
     CDS             complement(12293..12898)
                     /gene="dhaL"
                     /locus_tag="KMT45_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBE7003761.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(12926..13936)
                     /gene="dhaK"
                     /locus_tag="KMT45_000271"
     CDS             complement(12926..13936)
                     /gene="dhaK"
                     /locus_tag="KMT45_000271"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBE7003762.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(13911..14357)
                     /gene="dhaM"
                     /locus_tag="KMT45_000272"
     CDS             complement(13911..14357)
                     /gene="dhaM"
                     /locus_tag="KMT45_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBE7003763.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            14460..14780
                     /locus_tag="KMT45_000273"
     CDS             14460..14780
                     /locus_tag="KMT45_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBE7003764.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(14892..15761)
                     /locus_tag="KMT45_000274"
     CDS             complement(14892..15761)
                     /locus_tag="KMT45_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBE7003765.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(15857..17161)
                     /locus_tag="KMT45_000275"
     CDS             complement(15857..17161)
                     /locus_tag="KMT45_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBE7003766.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVSFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(17158..17667)
                     /locus_tag="KMT45_000276"
     CDS             complement(17158..17667)
                     /locus_tag="KMT45_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003767.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(17842..19977)
                     /locus_tag="KMT45_000277"
     CDS             complement(17842..19977)
                     /locus_tag="KMT45_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBE7003768.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(20060..21715)
                     /locus_tag="KMT45_000278"
     CDS             complement(20060..21715)
                     /locus_tag="KMT45_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE7003769.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(21717..22031)
                     /locus_tag="KMT45_000279"
     CDS             complement(21717..22031)
                     /locus_tag="KMT45_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBE7003770.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            22201..22461
                     /locus_tag="KMT45_000280"
     CDS             22201..22461
                     /locus_tag="KMT45_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBE7003771.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            22475..22789
                     /locus_tag="KMT45_000281"
     CDS             22475..22789
                     /locus_tag="KMT45_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBE7003772.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(23519..24073)
                     /locus_tag="KMT45_000282"
     CDS             complement(23519..24073)
                     /locus_tag="KMT45_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBE7003773.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(24199..26128)
                     /locus_tag="KMT45_000283"
                     /pseudo
     CDS             complement(24199..26128)
                     /locus_tag="KMT45_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(26434..27150)
                     /locus_tag="KMT45_000284"
     CDS             complement(26434..27150)
                     /locus_tag="KMT45_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBE7003774.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(27151..28974)
                     /gene="glpO"
                     /locus_tag="KMT45_000285"
     CDS             complement(27151..28974)
                     /gene="glpO"
                     /locus_tag="KMT45_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBE7003775.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(28988..30484)
                     /gene="glpK"
                     /locus_tag="KMT45_000286"
     CDS             complement(28988..30484)
                     /gene="glpK"
                     /locus_tag="KMT45_000286"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBE7003776.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(30620..32080)
                     /locus_tag="KMT45_000287"
     CDS             complement(30620..32080)
                     /locus_tag="KMT45_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7003777.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(32070..33365)
                     /locus_tag="KMT45_000288"
     CDS             complement(32070..33365)
                     /locus_tag="KMT45_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE7003778.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(33697..34374)
                     /locus_tag="KMT45_000289"
     CDS             complement(33697..34374)
                     /locus_tag="KMT45_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBE7003779.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(34404..35495)
                     /locus_tag="KMT45_000290"
     CDS             complement(34404..35495)
                     /locus_tag="KMT45_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBE7003780.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(35596..36030)
                     /locus_tag="KMT45_000291"
     CDS             complement(35596..36030)
                     /locus_tag="KMT45_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE7003781.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(36614..38071)
                     /gene="ascB"
                     /locus_tag="KMT45_000292"
     CDS             complement(36614..38071)
                     /gene="ascB"
                     /locus_tag="KMT45_000292"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBE7003782.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(38068..39963)
                     /locus_tag="KMT45_000293"
     CDS             complement(38068..39963)
                     /locus_tag="KMT45_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE7003783.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(40021..41796)
                     /locus_tag="KMT45_000294"
     CDS             complement(40021..41796)
                     /locus_tag="KMT45_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBE7003784.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(41846..43342)
                     /locus_tag="KMT45_000295"
     CDS             complement(41846..43342)
                     /locus_tag="KMT45_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBE7003785.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(43536..45158)
                     /locus_tag="KMT45_000296"
     CDS             complement(43536..45158)
                     /locus_tag="KMT45_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBE7003786.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(45486..46799)
                     /locus_tag="KMT45_000297"
     CDS             complement(45486..46799)
                     /locus_tag="KMT45_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBE7003787.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(47044..47268)
                     /locus_tag="KMT45_000298"
     CDS             complement(47044..47268)
                     /locus_tag="KMT45_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003788.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(47288..47710)
                     /locus_tag="KMT45_000299"
     CDS             complement(47288..47710)
                     /locus_tag="KMT45_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBE7003789.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(47852..48562)
                     /locus_tag="KMT45_000300"
     CDS             complement(47852..48562)
                     /locus_tag="KMT45_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003790.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(48580..49077)
                     /locus_tag="KMT45_000301"
     CDS             complement(48580..49077)
                     /locus_tag="KMT45_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7003791.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(49114..49566)
                     /locus_tag="KMT45_000302"
     CDS             complement(49114..49566)
                     /locus_tag="KMT45_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE7003792.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(49701..50306)
                     /locus_tag="KMT45_000303"
     CDS             complement(49701..50306)
                     /locus_tag="KMT45_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBE7003793.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(50756..51376)
                     /locus_tag="KMT45_000304"
     CDS             complement(50756..51376)
                     /locus_tag="KMT45_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBE7003794.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(51378..52247)
                     /locus_tag="KMT45_000305"
     CDS             complement(51378..52247)
                     /locus_tag="KMT45_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBE7003795.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(52270..52677)
                     /locus_tag="KMT45_000306"
     CDS             complement(52270..52677)
                     /locus_tag="KMT45_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBE7003796.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(52696..53985)
                     /locus_tag="KMT45_000307"
     CDS             complement(52696..53985)
                     /locus_tag="KMT45_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7003797.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        15294 a        11440 c         9266 g        17988 t
ORIGIN      
        1 aactattcac ttggcaggac ctcctactaa atcagcgaca aaacgtcggc tgaaagactt
       61 atttttttat ttttcttcta aacttttttc tacctcaatc ccccaagaat ctttcttgaa
      121 gtgattgatg atcaatggaa taacgaacat gaccactgtg atagctgcta ctaataatgc
      181 atcactcaat gtcgctgtac ctaatgtagc aaatacgaca acgatgatag agaagatcag
      241 catgcagctg actacccaag ctaatccgtt tccgttttta ccaattcggt aaggacgttc
      301 gaattctgga tgtttcttac gcattttaag caatgaaaca gcaatcaaaa tgtacacgat
      361 acaataaata acagttgtag catttgtaag tgtcaagaat acagcactta cgtcatccat
      421 tacaccataa agcaaagcaa ataatgaaat aacgattgat tgtgttagaa cgacatttct
      481 agatacgccg tatttgtttt ctttatggaa tccgaatttt ggtggaagga atccttcacg
      541 agctacttgg atgattgttt ttgatggtcc agtaacccaa gctgataatt gtaaaagcac
      601 accaataaat accatgaagc tgaagatatt tccgatcact tctggtaagc ctaggatttg
      661 acagtcaatc aagataggct gcgtgatatt tgctaattcc atttttccat ctggtacgac
      721 atttgctaca agcatcgcat tgatgacatt caataaaact aatccgatca aagcgataaa
      781 tacgcctttt gtataatttt ttgttgcgtc tttcaaacga ggcatgtaaa cagaactcat
      841 ttcgatccca acaaaaataa atgtgatacc agctaagtat ttcaatgaat caatatgttc
      901 caagtcaggg aaagcttttg accatgaaaa gtcacctaga tagccgtttg agactagtcc
      961 gactttgaag gctgccaaaa cacctaaaac gaacatgatg actactggga tatatacccc
     1021 tagccataca ccgatgttcc cgccgacttt tgccatatcg aatttcaagt tcaagatcgt
     1081 gatgatccag tagataacta aaatacaacc taaaacgaac cagtgattat tgccaagttc
     1141 tacattacca aatgtgtttc ctaacaatgg tcctaaagta gaagcgacca ttaccatacc
     1201 tgggaacatt tgcacccata atagccatgc aacgacaaat ccccatttgc tgcccagtgc
     1261 tgtttttacc cataactgag gaccgccttc ttgaggaagc atcgtggaaa gctcaccgga
     1321 aaccattgat atcggaccgg caaaaaacaa aactgcaaaa attgaataaa aaatcaatgt
     1381 ccaaccgact gcggctaacg taggaatact ccgaaccgtt gcacacagtg ccattgtcag
     1441 accgataaag gtcccaagac ccattttact tacttcagat ttactcaccg ttgacactct
     1501 ccttgatttt ttggataaaa taacggtgtc tttgaagtag tttcagcaat aaacgaaaca
     1561 attcagcgaa tatttgaggg gattctttga gtttattgct tgaagggttt cttcaaagac
     1621 aacctcaaaa caaagggatc gaagagctta ggtctcccct tgctcttcag atccaaacat
     1681 gcgtcagaaa cagttagtat tcaagctgtt ttctaacatg attaccttgc ttattttgct
     1741 tcgcttgcca ataattggtc tgcgtagatt ttttccaatt tttcttgaag agctgctttg
     1801 attttttcag catattcttc aaagtgttct tctttgttca tgtatggtgt cattacgctg
     1861 gcacgcaata cagttacttt tccggcacgg ttccattctt catctgagaa tcctagttgg
     1921 ttcacaaatt gtaatgggct gttgccatag tctgggatag caaagtcagt atgtgaagtt
     1981 aagaactcat taccgtaaat gcttcctttc acgtatgaag aataatcata tacgtcatgg
     2041 ttcaatttat tcattgcaac taagtcatcg ttgccttttt ctttgaatac atagtctacc
     2101 atattgaaat ctggataagt taatggatga acttcgattt ctttgtctcc tactttaaat
     2161 gacaagtcat tcaagaagtt gtagaaacga tgtgcacctt cgattgatgc acccattaat
     2221 ttaccatatc ctgtgacatt caaaggtaag acatggtgtg ctgcccaaac gctggctgct
     2281 gttgcccctg cttttgaacc ttccaagata taagccccaa gtaatgctgg gatatctgcg
     2341 cctttttcaa atacatatgt cgcaaagtaa gagataacgt ctctcatgcg gatatctttg
     2401 atgacgatac caccggcaga atatggaaca tagcccattt tatgtgggtc aatcgttaca
     2461 gattctgctt cttcgattgc tttatatgca ctgtgtactt cttctaagat ataatctttg
     2521 ttttctgtaa agacattgta tttgtaatga acatctttta aatcttcaaa tggaatgaag
     2581 ttgttgtctt cgtctaagaa aatcgcacgg ccgtatccgc cataagcagc atctacgtgt
     2641 aagtagaagt aaataccgtc tttttctaat acgcgacgca atgcaacgat tttatcgata
     2701 ccgtcgattg ctccttcttc tgttgatcct acaacaccga ctacacctag gattggtgtt
     2761 ttttcagctg ctagaccacg aacgattttt tcaagttcgt tgatgtccat gcgatagttg
     2821 tggtcaactg gcactggaat cacttggtct aaaccgatac cgatgatgtc ggcagctttt
     2881 agccatgaat agtgtttagt ttgtggaacc aaccattttc ctaatttttc taaatgtttt
     2941 ccgctacgtg ctgagtgagc tttgatttcg tcgatttttt ctgggacgct gtctaacaga
     3001 ttcatgattt cttctgttga caagttcatt aattcccaat cgctttttcc agcaactagt
     3061 tcaggtgtca cttctttcat tgcaagcggc aatgatttga tgttgcgggc ataccaaagt
     3121 ccttctaagt ttgctaaaga accatcagca acgatgtggc cccagccatc tttatagctc
     3181 attaattttg caaattccat tcctacttct tcttccattt ggcttgttgc aggagaagat
     3241 tcataggcaa cgttattacc attccatagc atagcaaaat tgtacgctag taaagatggc
     3301 atcaatgttt cagagttcat atgaccccag taacgtccgg cattatgcca aggaacggaa
     3361 tgggtacgca tccgcgctga aatttctgat agaacatcct ttgttttatt cacagtgtgt
     3421 tcaaaactag cactgctttt ttcttctggc gtaataatag gcatgtcttg aggcatgtag
     3481 ttttgacgcc agcctaaatg ttcatccacc aattcattca acaaagcttt atagatttgg
     3541 ccgttctcag ctttgtcccc aatgaatagg gcatttaggt ttagatcttt cgacaatgat
     3601 tcactcatag tgaaaccctc ctaaaaaaat atttaaattt tgtcgagccc tacattacac
     3661 ctgttcttct attctgtaaa ctaagttttg gcctgatttt taatcatatt ttaatatgta
     3721 tcaattatcc gaattgttca cacttttgaa ataattaaaa aaaacgaggg aatttacaaa
     3781 tgtaaactcc ctcgcaaaga ttgtattaac gacaaccagc gttctatttt aggatgggtt
     3841 tcaaaattgg aatgttgtaa tagtatttcc ctttttcaag gtaaaaactt actaaattct
     3901 aatcaattac tttggccaaa aagtaatttt tcttaccttt tcgaataacc acaaacttac
     3961 catcgaattc atcggatgga tttatgacaa aatttaaatc ggtaacgcgg tcaccgttaa
     4021 tacttatagc tccgtttgaa acatcttcac gtgcttgacg tttagatggt tcaatttttg
     4081 tggaaacaag cagttccacg atattttcag gtgtgcttga aatttcaacg ttcggcattt
     4141 taccgaatcc ttgcgcgatt tcttccgcat ttagttcttt aatatttcca gagaacaatg
     4201 cttgcgttat tttttgcgct tctttcaagt cttcttcact atgcacaaat cttgtaactt
     4261 cttctgccaa acggcgttgt gcttcacgtt tctcaggttc tgtctctact tttttcgcta
     4321 agtcttcgat ttcttcttga gacaagaaag taaagaattt caagtatttc actacatcgc
     4381 gatcatcttg attcaaccag aattggtaga actcaaatgg tgaagttttc ttcggatcaa
     4441 gccaaacggc accgcctgca gtttttccga atttcgttcc gtcagccttc agcatcaatg
     4501 ggatcgttaa accgaacgct tctgcttctg gtccttctaa ttttcggatt aggtctaatc
     4561 cagctgtgat atttccccat tggtctgctc caccgatttg caattgcaca tcgtatgttt
     4621 tatgcagatg caaaaagtcg atagattgca ggatttggta agtaaactct gtaaatgaga
     4681 ttcctacttc caaacggctg gcaacgatat cttttgctaa catcgtgttg atgttgaagt
     4741 ttttaccgta gtctctcaaa aattcaagta aagagatttt tgacaaccag tcgtagttgt
     4801 tcacaaatgt gatatttgca tctttaccaa acaattttct catttgattg gaaagtgcgt
     4861 ccacgttatg ttgcacttgt tccatcgttt gcaacacacg ttctgttttg cggccgcttg
     4921 ggtcgccgat cgttcctgtt ccgccgccaa tcagaatata tggatgatgg cctgccaatt
     4981 ggaatctttt catcatcata aatggaatca aatgtccgat atgcatcgaa tcaccagttg
     5041 gatcaacgcc gcaataaagt gaaatgctct tttcttcaac gagctcgcga agtctttctt
     5101 cgtttgtttg ctgattaatg gcgtcgcgcc aagttaattc atcaataata ttcattttac
     5161 tgacctccgt gtagtttttt tatgaaagca ggaacctgct agcatatgta attaattatt
     5221 tcttcaggat aggataggaa ataaaaaaag tcccttggca tataagccaa gggacgaaat
     5281 ctcgcggtac cacccaattt gtatgtctag atatccaaca tacctctcga acatttaacg
     5341 ctgtcgcgtg gcaatcactg ccaaagactc ctgaaatgta attcgcttta aagcatgtac
     5401 tggtttgcag cgaccaccag ctttctgaaa cattggcttt aacaactact tcgtttcgat
     5461 cttcgtcttg ttattattaa tatgtgtcta tcatagagcg caactttttc tttgtcaaca
     5521 gaaaaaggat atttttttta aattcctcat tttttctttt ttgaatccgc tctaattccg
     5581 aacattttat tttcaattat tgttaaaaga aataactttt tctgtatttt ttctgcaatt
     5641 tatcgagaag tcactttact ttgcctctta ttctttcttc gttacaataa ggaatgggac
     5701 aagtaaaaaa atgaaaaagc aagggacgag gtatatggaa aaaaagcaaa aaattatttt
     5761 aggagccact tctgctttaa ctatcgttat tttaattcta agtttgtttt ttgtcatccg
     5821 atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa ataacagaaa aagcaaaaga
     5881 accgacagat gataagcaac aactagttac tcaagcaaaa aatctggctc aaagctacga
     5941 ttatgaccaa gcgattgcgt tattggaaaa agatagtaag aacggatcta ctgaaacaca
     6001 acagcttctt caaacactaa aaaaggagaa agaacaactg atcgactggt ctgatcctac
     6061 tcagatctct catgtctttt tccacagctt gatcgtggac ccgcaaaaag ccttccactc
     6121 tcaacaggca caaggataca aagattatat ggtgactgtt gaagaattca accgttcgat
     6181 cgaacaactt tatcaaaatg attttgtttt agttaatttg aatgatttga tccaaaaaga
     6241 cgaacaagga aatctttcct ttaacgggct tgcgctacct gaaggaaaaa aaccattggt
     6301 tctttcacaa gatgatgtca gctattatga atatatggat aattccggtt ttcctagccg
     6361 attggtcgta tcaaataaca atgaaatcaa aaatgtctat aaagagaaag ataaagaaga
     6421 aatcggtgac tttgacatgg ttcctctgat cgatgccttt gtcaagaaac atcctgattt
     6481 ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt tacaacggag ttttaggtta
     6541 tcgaacatca aaatctcaat atggagacaa tgaaaaaaca aaaaaagaga ttaaagaagc
     6601 taaaaaagtg gcggaacagt tgaagaaaga cggctggagc tttgcttccc atacatgggg
     6661 tcacttgaat atgactcaag cttcactaga agatgtaaaa aaagacaatg aattgtggca
     6721 gcaagaagtt gcacctatcc taggaaaaac aaatattctg atttatccat ttggtgcaga
     6781 catcagcgac tggcagcctt attcagcaga aaatcaaaaa tttgcttact tgaagagtca
     6841 aggtttcgat attttctgta atgtcgatgc ttctacacca gcttggggac aattaggtac
     6901 ggatttctat cgaaatgctc ggatcaatat cgatggcatt cgttttcaat ctgatttaga
     6961 cggaaaaaat cctattcttg atccattcat caatgtccat gatgtctatg acaaagaagc
     7021 acgcgaatct gcttgatttt acgtcattta tgaaccaaaa aagccgaact gacattttat
     7081 cgtcagtttc ggctttttta tgctttattt cttcttcggt gcgattccct tgatcaggac
     7141 ttctgtccat tctgctgtat atggaagcag ttttgcgtat atctccggct tagaaattcc
     7201 ttctaccatt gcttgaaaat agaggatcaa gaactcatcc gaatatttca aatccacttt
     7261 tccttctttc cgccctcgat ggaaaagatc aagcatgata ctgaaacttt cttgcgtata
     7321 cgcatccatc gcgtcagaaa attctttttt ttcttttttt gtatagtatg tcatcaaatc
     7381 gatataaaat tgtttgttca ctcgattgag ataacggatt ttgttttcac tcatggcaat
     7441 cagtgtttct tcaaatggaa gattttccgc aatcaacgct ttcgcttctt gccctaaatc
     7501 attcataaat gcaaaaaata cttcataaat cagcccttcc ttgctgccaa aatatttaaa
     7561 aatcgtcgct ttacttacct gagcgttctc agctacctta tccattgtca ctccagcggt
     7621 tcctatgtct gtgttcatca agtcaaaagc tgcttgtaaa atctctttct ttttctcttg
     7681 tgctctcttt tcaaaaccgt tcatttgtag agcctcccgt aattttaaac ttttataata
     7741 tatatagttc aaatttattc tgaggtttat tataactatc tattgagaaa gtatcaagct
     7801 agcaattcct tgtttatgaa ttcattgaca gaaatttata aaaagaatat tatgtactaa
     7861 aataataaaa aaagttttat aaaaaggtat aaaacctttt tattaaacta tttaatcttg
     7921 tttagtatat atttttagaa aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac
     7981 acatttgaaa aagagctttg gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca
     8041 ttcaggtgaa gtcgttggat ttatcggacc aaatggttct ggaaaatcca cgacgattcg
     8101 gacactttta gggattataa aaaaagacgg cggaacggca acgatttttg ggcaagatgt
     8161 atgggaagat agtttatcta tccatgaacg tctttcttat gttccaggcg atatctcttt
     8221 gtggggaaat ttgactggag gagcgatcat cgatctcttc atgaagcttc atggaaaagg
     8281 gagtgctcaa aaaaaagatg aactgattca acgtttctct cttgatccta agaaaaaagc
     8341 aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg attgctgctt tggctgttga
     8401 ttcggatctc tatttatttg atgagcctac ttctggttta gatcctttga tggaaacgat
     8461 tttccaagaa gaagttgaaa aattgaaaga acagggcaaa tccatcctac tttcttctca
     8521 tattctcagt gaagtcgagc gactagcgga taaagtcgtg atcatacgcg aaggaacact
     8581 cgatgaagaa ggaacactcg atgaattgcg tcatttgact cgttcgacca tcactataga
     8641 aacagaagca gagaatagtc acttagcaca attgaacggt gtccatgact ttgtgcaaaa
     8701 aggtcacctc gctcaatttg ctgctgataa cgaagctttc gatgtgattt tagctgaagc
     8761 gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg actcttgagg atttattcat
     8821 gagacattat gaaggatgag gaggaagcga aatgactgat aaattttcac gctggtccac
     8881 ccttttttct caaaacctta gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt
     8941 cactgctttt tctggtggtt ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt
     9001 agctggatta tacgaaacct tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc
     9061 agcttcgaat gctgctgagt atactttagg agcgatgtat gcaaatgaaa tgttgctttt
     9121 ttgcagtttg tttgctgctg tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa
     9181 agaagaactt ggtctgattg aacttattcg ttcctttcga gtcggacgtc aagcaaactc
     9241 tttagctgtc atcgctgaaa gtattctgat caatttgctt ttagcaatat cgatcagctg
     9301 tacgatgatt tttttccaag cggattcgat cactgcagaa ggtgcttggc tttttgggct
     9361 atctgtaggt agtatgggga ttttggggag tgccgttgca ctgtttgctg cacaactgat
     9421 gccaagttca gccagttcga atggtctgtc tttagcgttt ttaggtggac tttaccttct
     9481 acgagcaata acagatgtag ctgattcctc cctttcgatg tggaatcctt taggctggtc
     9541 ttatttgacc tatccattta caactaacaa ctggattcct ttgctttatt tgtttttgtt
     9601 cagtctaatc ttgataatca gttcatttgt tttagaggaa catcgagatt tgaacaccgg
     9661 ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg
     9721 tttattattc tatctaaata aagggatgac aatcagttgg cttatcactt ttggtttgat
     9781 gggggcggca tacggctcta tctacgggaa tatgcaacag tttttagaaa gtaacgactt
     9841 aatgaaacag atgtttatcc aatctggcgt ctcaattgaa aaaaacttta caagcacgat
     9901 catggtagtc ttgatttgtc tagctgctat tttaccaatt gctagcatca atcgtttatt
     9961 tacagaagaa acccacggac gtatgaatca gctgttcgca acaaaaattt ctaggaacca
    10021 actttattgg accaatatgt tactagctat tctatttgct atattggcaa tcctgattag
    10081 ttctggtgca ctagggcttt ctgctttggc ttcgatgaag gatgcgaaag acatggatct
    10141 attgatgttt cttaaagccg ggttcaattt cctgcctgcc gttttagtgt ttatcggaat
    10201 tagtggcttt ctattcggat ggcttcctcg aatcggtaaa cttgcttata tctacttagc
    10261 ttactccttt gcactcaatt actttggcgg aattttggat ttaccagaat ggttttccaa
    10321 aacagctgtc cctagctggg ttccacggat gccaaacgat tcttttgatc tttccgtgtt
    10381 ccttacgtta tcatgtatca gccttgcact gttcgtcctt ggttctattg gttataggcg
    10441 aagagactta ctagaaggat aataaaaaag catgtacaat ttcatgctag cctccaaatc
    10501 tgggtcaaaa atccaacatt tggaggtcat ttttttattt acaagtcttc taactgctct
    10561 ttatcaatga tccagacttc tcgattcttt ctttttacat aaccattctc ctctaataat
    10621 ttaaattttc tagataaagt ttcgggggtc gtaccgataa atgcagcaag ttctttcatt
    10681 ttcattggca ggaaaaagtg atccttccct gtcgctttag ctaagtcaag cagatagaca
    10741 gcgactcgtt cttctacttt ttccatcatc aaaaaagttg tctgctgctg tacttttgtt
    10801 aatttctcaa cgttgattgc taaaagtttc agactaaggg cgggataatg cagtaagagt
    10861 tccgagaaat ctttttgtct caatatgcaa atttccgttt tttctagtgc ttcgccaaat
    10921 aaagactcat ttggaacgcc tagcaactgg ctctctccct catatccgcc cggctctatt
    10981 actcgtaaaa gctgttcctt tccgctagca gataattgat acactttcat actgccggag
    11041 gccacgatca ctaattgagg ttctccattc ggcgcaagaa tctgttctcc tttttccaca
    11101 tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt gctgtactaa gtgattgaac
    11161 aaaggaacaa atgaaacaca taaatgatca tgactcataa tctctcctcc ttctaaacag
    11221 ctatttctaa taaagaaaca taacttccct tattccaact gataattttc ttttatcata
    11281 gcataaacgt caagcgaacc agataccagc aaatagagtg gatttctttt tgtcccttgc
    11341 tttattttcc ttcttttgag accttaagta attgattcct atcaattttt taaatattta
    11401 ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg
    11461 cattgcttat ttcgtgttgt ctattgaaat ttttccgcac ctagcttttg atatagtggt
    11521 gctagttctt gtaaggtttc agcggttatt tgctcaacgt gttcgatttc ttgcggatag
    11581 aagaatagtt tacccatttg aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt
    11641 ttctttgtat cggacaaata gtcagacaaa gctaagtggt cgtcaagttc atgttctggt
    11701 tgcggccata tgtaataatc cttgatatcg actgttttgc tccactcttt cagtatcgca
    11761 agcaactggt tatacgctgc aatgctacca tatcgttctt cgcttttata atgttggtac
    11821 atcaaataga tttggtattg tttttgattc aacaaaactg ctagacaggc attctcgtct
    11881 tgacgagatt cgcttcggta cgcgctccaa aaatgactgc gtaaattcca gccattcgtc
    11941 cagctttcga ttttaggttt gctcatgccg tacatatcag gcaaaagtgc agctgtctgg
    12001 agctgaatct ctttccattt ttgccagtgt tctttatatt ctttcttgat ttgttcaatc
    12061 ttatctgttg gatggctctc tttgagctgt ttgaatgcgt agattggtcg atcaaacaac
    12121 tgataatctg attttccaag ttccattgtt tcgcctcttt ccgttatctt ttgtattcta
    12181 cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat
    12241 aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca gttttattga acttaaggtt
    12301 gattgatcac atctaaaagc gttgaaaata aaatggcact agaagcagct cctgggtcga
    12361 tatgtccgat tgcacgctct cctagatagg aagctcgtcc ttttttagct accaagactt
    12421 tggtgtgttc ttttgcctgg tcaatgattt cttttgttaa attccctgcc ttcaatgcct
    12481 ctgtgactgg cagccaaaca tctaacatcg ttttatcgtc agcttctgcc tttcctcggc
    12541 tttgaatacc cgctgttccc tgctcgatca cttcgcctaa tttttcttga gaatcaatag
    12601 aatcgacctc ttttgtcgct ttcatcatgt tcatgaatgc agatccgtat aacggaccag
    12661 aagccccacc gactttcgat atcatcgcca tagagaatac tttgaatgta tcgctgattg
    12721 tttcaggatg atcggcttga aaagcttctt cataagctga aacccctctt cccatgttat
    12781 ttccatggtc tccatcgcca atcggggtat ctagatcact gagatattgt ttgttctgat
    12841 tgattgcctc tgcaaattgc gtgagccatt cttggatttc ttgaacggtt agtttcatat
    12901 tatcaccctt ttatcttgag attttttacc atgaaatcgt ctgtacattg ctttctaaag
    12961 aatctttcca ttgtgtctcc gtcaagtcga tcagtgttag agaaagacct tgcatatcta
    13021 gtgaggtcat aaaattaccg acttttcgga aaactacatt gacgcctctt tcttccaaca
    13081 gagctaaaac atcattcatg aacacgaact gttccatcaa aggcgtcccg cccattccgt
    13141 tgactagtac acctgcttct tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa
    13201 gttcctttgc cagtacttta gaaggctgca tcttttctcg acgatagcct ggctcaccgt
    13261 ggataccaac accatactcg atctcatctt ctcctagttc gaatcctggt ttccctactt
    13321 ctggtacagt tgctgctcga agcgctagac cgatcgtctt agttgcctgt acgacctttt
    13381 ctcccagttc tttcaactca gctagggaag caccgtttct tgctgcatcg cccacgattt
    13441 tgtgtacaag gacagtacct gcaactccgc gttttccagc agtatacgta ctattttcaa
    13501 cggcgatatc atcatctacg atcacacttt ctacttcaat atcgtccata gcagctaatt
    13561 cctttgccat atcgaagttc aatgcatccc ctgtataatt cttaacgatc agcaaaactc
    13621 cttttccact atcggcttcc ttaatagccg tctgaatctg atctggagtt ggagaagtaa
    13681 acacatcacc aagcaccgct gcacttaaca tcccttcccc tacaaacccg gcatgggatg
    13741 gttcatgtcc gcttcctcca ccagaaacca aaccaacttg ttccgctttc tgattcttgg
    13801 ctacaacacg ggaagattct acacgatgaa caagttctgg atagcttttt actaatcctt
    13861 ccagcatttc ttcgacaata ttgcttggat cattcatgat ttttttcaat ttattcccat
    13921 cctttctttc cggcattttg tgcttctgtt aagatttgct gagtatcatc tgttataccg
    13981 gcagtgatgg ctgctgcaaa agctccttcc actaaaggcg catccaccag atgataacgt
    14041 tgctgctgct cttcttccaa catgtctttt gccagttccg agttcaatac cgcgctaccg
    14101 atatccgcaa aaacaaaata actagctgca tcgcttgatt cattgactgc ttctacgatt
    14161 ttcatcgggt ctgatccgat ctcgccattt attccgccga gagaaaaaat ttgcacctct
    14221 tcggattttt gcatctgctc aatcatttct ttaataccat ctgttacttt ttgactatga
    14281 gaaataagca aaatactttt tttcacaaga atcttccttt cttttacata gttgctaacg
    14341 attggtttcg ctttcattaa tgaacaattc aagtatataa taaatttcta attttgggaa
    14401 aatatgtgta gtgcttcttg aagtttgtta tactttaatt aaaagagagg agtgatcgaa
    14461 tgattacaac aacaacgaac agtgtagagg gaaaagccgt agtagcttat aaagggatcg
    14521 tttttggtga ggtcattact gggatccatg catttaaaga cttggaggcg gggttgcgaa
    14581 atatctttgg cggccgctca aaaagctatg aaaacgaact aatgggtgca agagaagaag
    14641 cacttgccga aatggccgaa cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa
    14701 aaatggacta tgaagtatta ggcagcgaca atggcatgct gatggtcaca tgtagtggaa
    14761 cagctgtctc cacgagataa gtaggggaca tctgttttaa taactaaaga gagatgggac
    14821 atgtagtggc actcaagaat tagatttttc agtctaactt cgaggtactc taccttccca
    14881 tcctctcttt tttatttctt tttgcggatg attccgtaag ctgctgcgac taacacaccc
    14941 gcccctgcta aggaataact gacagaagct gttttttcat ttgtttttgg caaagtctga
    15001 cttgttgatt tggatggctc tgtcgtttgc tgaactgttt ctgtcgtgct tccagaggtc
    15061 actgattttt ctcctgccga atgcaaaata tcttttattc gttggaaact agctgctaag
    15121 tcagcttttt ttgcttcaaa atcattagca actgtttgtg cttcgctgat ctttccgtgg
    15181 attgcttgac tggccgctgt gatatccgct tgagtttgac ttagctttcc agaagctgtt
    15241 tgagcaacct gatttaattg ctgattgaat tcttttgctt gttggatctt cccgtcaaga
    15301 tctgtttgga cagcttccgc ttttgctttc gcttcggcaa gctttttctc aagttgtgca
    15361 cggtcatttc ccactttatt tttaagatca gctaaatctt gatcgattgc tgcttggtct
    15421 tctttcaatt tgttttttgc agcttctaat gcttcttcga gttcttttct tttattttcc
    15481 aagtcttgca aatgatcgat ttggtcagaa gaggtagaac tatttattga tgtatcttct
    15541 gaagatgaaa tcgtttctcc tgaattatct gtcggtacat tggaagtcat ttctgtttcc
    15601 gaggcaccgt tttcagcacc agaagagctt atggttgttg aaggttctgt ttgagcaggt
    15661 tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa tactggaaac agcaattggc
    15721 cagattaacg gcttcattct ttttttcatc acttttccca tccttactac tttttaacta
    15781 atttcttatt ataagcaaaa atgtttggaa gaaccataaa cacaacaggc tgtttgtcta
    15841 aaaaacggtg catcttttat ttttttgtac gttccgggct tttccatgtc ccgcttccat
    15901 aacctaaaat cgttttgact gctggaatga aagtcgtcac gatcgttatc gcgttcacta
    15961 tccagaacaa aagcatatat agtggtgcga aaagaaggta tttcaatttt cttcgtcgat
    16021 catctacaat cagcgaagct agcaactgga aaaatcctgc gaccatttca aagcagatga
    16081 acacaaaact gaatgtaaaa gtaatataaa tctgttcgta gtttccttga tatacataat
    16141 gcccgattaa tccagcaaaa agaactacgg aaatacaaaa gaatattgac cagacaatac
    16201 tcaatgtttg atcaatgaag atgatcgttc ttccaatatg tttaaaagga tggagcatca
    16261 cttttttgaa attcgtcagc cagacttctg ttccaccttt tgcccaacgt tttctgtgtc
    16321 gatatagcat cttcaacgtc acaggtactt ccatgaaaaa gataatctca ggagcaaaaa
    16381 cagataacca gtcatttaac tgatgatccc aagcaatact gatgtcttct gtcgctcgat
    16441 cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg atacatcgta tttgctccac
    16501 tgtacgcata aagaccaccg aaaacagctg actgtgttcg tttaatgatg cccacgatac
    16561 ttgagaattc aaccgtttga gattttgcaa tcaatttggt acgattttgg acatccatat
    16621 ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat gaaataattc acatacctaa
    16681 tcagtgcatc tggctccggt accgtgtctg catcattgct taaaatcaat ttcccttttg
    16741 caaaggcaag tccgatatta aacgcgtggg cttttccttt atttttttcg atccgaacca
    16801 cacgaagatt agcgtatttt ttcatcagtc tagctaatat ttctggtgtt tggtccgttg
    16861 aaccatcgtc tgtcactaat acctcatagt tgtgataatt caatttcgtc atcaaatact
    16921 cgattgtata ttcgatcaca atttcttcat tatgggctgg caccatgatc gtgatgaacg
    16981 gttcgacact caagggaaca tctacccatt ctttttgctt atgtttgtat aaaaacacat
    17041 aacaccagac accaataaac gatacaaatc cgcctaaaat cggataagaa acgagaaaaa
    17101 aatacataat agacaaacaa gtatctaaga ataaattacc tgtatgggtg attgagatca
    17161 tttcagttcc accccatttt ctttatacaa atctgcaatg aatgtcgtat ctagattttt
    17221 ctcttcggag acagaataaa aacggaccgt ttctctctct tcttttgttc caaaacgttc
    17281 cgtatacact tcttcaagta attcttgtct ttttttcaat ttttcatctt catattcaaa
    17341 agatttattt acttttttgt atagatttcg attgttccac ctagtcaata caaatgaggt
    17401 aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt aagaaataca gcattttctt
    17461 ttctgataaa aaagcgtaca aggaaaaatc cttggctctt tctggtctcg ttaatgggaa
    17521 aaagacccat ataaaaggaa gaacaacgat catccatcct aaaagagcca ctaacgtttg
    17581 ccaaatcttt aaccagtaat taccttttga aaaatactta tctttcacca aaattgatgc
    17641 aacttcttct cttgttactt ttttcatatc ctcacctcac gtttttttgt tacgacgtag
    17701 gagtttacca tacatttcat ttcttttcac ataaagtgtt cctaaatttt tcctaagaaa
    17761 aagaacaaaa catttctcat cagcaaatga aaaatatttt gttcttttta tcagttgttc
    17821 tttttatttt ttatctctta attatgttgc tctttccaat acgacaatgc ttttcattcc
    17881 tctagatatc gccgtgtaca tttgtctgcg tcttctttgc tcatctccat cgttttcaaa
    17941 tggatataag attacatgat caaattccag tccttttgct agactcaaag gaatgatttg
    18001 gcagttttct aatccatatg aatttttttt aatcgattct tgtagcgttt gggtttcctc
    18061 ttctgtttgg gtgataactg ccgctttttc tccttttaga gaaaccaaaa tctcagctag
    18121 tattttcaac aattcttcag aggatcgaaa agagcggtat tcgggttctt ttccttgcgg
    18181 tcggatcgat acgatgtcga tcttctcttg atccacagca taggttttga aaagctctgt
    18241 gattgctccg ctagaacggt aactatttct aagatcatac cgatggatcg gcagatggta
    18301 gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa gaattaaaga ttgcctgatt
    18361 ctcgtcacct actaaagtaa agtgagtttt tggaaataat tcgatcaata gacctaattg
    18421 tgccagggta taatcttgta cttcatcaat caatagatag cgaaatgctg gtacatttac
    18481 cttttcaaca agtaaatgac gaatagctaa tataatgact gcttcatcaa cagtacgtgt
    18541 gggttgccaa gcataagctg agccatatcg tttttcataa attctttcaa acaactgatg
    18601 ctcatccacc catgccattt cttcgatttg tctggttatt tttcgatatt ttttttgtaa
    18661 aagtttcctt gcataagcta caatactttg ttccgaatca tcagaaatca atttaccgaa
    18721 taattccaac tgcctatctt ctgtcaacga gagtacctga ttctgcacag caggactttt
    18781 tgcttgtttc aaaagatgat tcttccattc gctcatcagt gcctttttcg ttgcttgaag
    18841 cctatcgatc aatttaggat gagcaggaac tttctcatat attttttcga tcgttttttt
    18901 agaaaggact gtttttccat ttttagtaag tccacggata aaattcggat gatctaaaaa
    18961 caaaggatct gattgtttta gattatcgat aaactttttg ctacgcaaac gttctgtctg
    19021 ctccgataca ttttctcccg tgatacgttt gaaataagcc tcttcacctt ctatttcctc
    19081 tgctgaaagc tgtgaaacca gctgtatgat ggttagattc agtggatttc tttcacctaa
    19141 tgatggtagg acattcgaaa tatagtcgat aaaccgatga ttaggtgata gaatcagcaa
    19201 gtcatcggat gtcatcgtat ctcgatataa atagagcaga tacgcaatac gctgcatgat
    19261 agcagaggtt ttaccacttc ctgctacgcc atttaccaag atattttgac ttttcacatc
    19321 ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg atagccttca tttcattggt
    19381 ctcatcttgg cctaagactt ctaataatac gtcatcttga atagctacag acgtatcaaa
    19441 aaacttgacc aaacgatctc tttccaaaag aaattgtcgt ctttctttaa taaccgtatc
    19501 gattttattt tgacgaaccg tataagaaga tggaccaatc atgttattgt aaaaaagttc
    19561 ggcaattggt gaacgccaat cataaacaag attttcatct gcatcatctg tgaaatttgc
    19621 tgtaccgata taaaaggatt cttttggctc attatccaga aagtctacaa cgacttttcc
    19681 aaagtaggga cttttcaata aacgttcgat ctttgctagc tgattttctg cgtttttctg
    19741 tttgatattc atctgatcga tttctctatt tttcatttca atcatcgaaa aagtatccag
    19801 gttatctaag taactgtcaa aattcaatcg aagctcgcca gccatttctt tcaacgactg
    19861 gtttccttct atttttgccg tatccatcga ttcccgcagt tcgttttttt tatcaattag
    19921 cttttgatac acgttcttca gatatgcttc ttctaactgt ttttgtgtgt tgaccataca
    19981 cattctcctc tttttttaaa gtggtggaac cgattagttt acctctttta agagtcgaat
    20041 gcaagctttt taacgagtat catttgttag gatatatcag ttcttcaggt cggacagttt
    20101 gatataaaga aaatgcacca tcaaggtttt ttacgtggta gccagactgc ttcaagattc
    20161 tttccgctag atagctacgt aatccgctat gacagctgac gatatattct tgttggctgt
    20221 ctaattcatt taatcggtcc cgaagttcat tcaaagggat atgtttggct tccttgaatt
    20281 tgcccttttc caattcttct gctgttcgca catctaataa aatttttcct tttgctagtt
    20341 ctgtcggcaa ttcatgccat tgtacggttt cactaagccc ttctgcaatg ttcattgctg
    20401 catatcctag catgtttact ggatctttgg ctgagccaaa tggcggtgca tacgtcaatt
    20461 ctaattccgg taaatcgaag atcgtcaaat gccctttgat tgctgttgct aaaatatcga
    20521 tccgtttatc cacacctttt gccccaacac cttgtgcacc ataaatctcg ccagtctctg
    20581 gatggaagat caacttcagt aaaatatctg ttgcttctgg ataatagctt gcatgatctt
    20641 ttcctgatac atgaatgact tcaaaagaca gttcgttttc tttggctgtt ctttcactta
    20701 aaccggtgga tgcggcagtc attccaaacg ttcgtataat tgctgttcca ataccgcctt
    20761 gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg tctaccttga cgattggctg
    20821 gtgaagcaag agaaataagt gcatcttttc cagtgatttg ctgttttaca acgatagcat
    20881 cccccaccgc ataaatatca ggaatattcg tttgataacg ttcgtctact aggatacccc
    20941 cgcgtatgcc taattcgatt ccagcggtca ctgctagctg gttatcagga cgaacaccaa
    21001 tggataaaat aactaaatca gcagggatgg tacgcccatc atccagtcgc aatcgtttcc
    21061 cgtggtcttc aaatccagcg acagcatgag aagtaatcac ttctactcct tgatgagaca
    21121 attctttttc tataaaagct gccatttctt catccagtgt tggaagaata tgtggtgctt
    21181 gttcgatcac catgacttct aaacctcttc tttttaggtt ttctgccatt tccaatccga
    21241 tgaatcctgc accaacgatc acggcacgct ttggctgttt ttctaatgca tgcatcactt
    21301 tatcgatatc tggtacattt ctaatagaaa aaacattgtc ggcttctgtt atccctggaa
    21361 tcgatggaac aactggtttt gcaccaggag ataaaaccaa tgcatcgtac tgttcaatgt
    21421 gtttttgacc ctcatgtacg acttctaccg ttttgttttc gggaaagatg gctgtcactt
    21481 catgatttgg acgcacatct aaacaaaaac gagcggataa tgattctggc gtttggacaa
    21541 gaagattttc ccgttcactg atttctcccg aaagataata aggcaagccg caatttgcaa
    21601 aagaaacata cggtcctttt tcaaaaacaa cgatttctgc atcttccatt aatcggcgaa
    21661 gccgtgtagc agcagacatt ccacctgcta cacctccgac aataacgatt ttcatcttat
    21721 ttccctccta ctgttcgatc aaaccattga ttcatgccac cacgtacatt cactacatca
    21781 taaccagatt tctttaattc tttagctgct tgtttactcc tcatccctga ttgacaaatc
    21841 acatagacgg tcttttcttt gtctccatca tatctgttta tactttgtaa tggaacattt
    21901 ttagctcctt ttatatgccc gccccggtat tctgcaggtg ttctaacatc aagtaacgct
    21961 ggattttcca aaagcttttc ctttaattct gggactgtta tcgattttat tgattgaaat
    22021 agcaaattca ttttctgcac ctcatttaca tttttataaa tacctcatgg ggtatattaa
    22081 acaggagctt tattaatatg tcaaccagaa tgactcttca tttttttcac tactttttat
    22141 atttgcataa aataccgata aggggtatta tctaattata aaaatagagg agaatgtgtt
    22201 atggagattg ataataaaaa tatcctgaac cgtttgaaac gtacagaggg acaaattcgt
    22261 ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg atgtcatcac tcaattaagc
    22321 gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg ttgcagataa tctgaaaaat
    22381 tgctttgaga aacctgatag taatccagaa gaacaagcgg ctaaactaga acaagcgatc
    22441 aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa gtcgttttcc atattgatga
    22501 attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac ctgataaaag cttctccaga
    22561 aacgacgatt gtcgtatctg taaatggcat cgcgattacc ggttatctag actctgccaa
    22621 tgcagaattc ttagatttgc aaggagtcgt ttttcacgct tgcgccaatg ctatgcgtgc
    22681 taatcatatt tcagaaagtt ctttacctga acaagtaatc gttgtaccag caggtgtcct
    22741 tgatttagtt gaactgcaat cccaaggtta tgcttatatc aagccttagt cagtagaggt
    22801 tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt tttcgaagaa gctacttctg
    22861 gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca tcaagtaata agaacagcca
    22921 aacaaaaata gcttctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt
    22981 ttgaacaccg tttaaggaag aggttgtgaa atcagcggtt tgacctaagt attagagaaa
    23041 aaaacgggaa aatagctcct catattttgc cgttttttga gctaataccg aaggtcagct
    23101 gattgaacac cgtttaagga agagattgtg aaaaagtcgc ttagtttcga gtattaagaa
    23161 aaattttgtg aaaatggatt tctacatttt tgcataaatt tggcttaata ccgagaaact
    23221 ggcttttgaa taccgtttaa ggaagaggtt gtaaaatcag cggtttgacc tgagtattag
    23281 agaaacaaac ggaaaaatag ctgctcatat tttgccgttt tttgatctaa taccgaaggt
    23341 cagctgattg aacaccgttt accaagaggt tgtgagccta ccatttaact ccgaatcaca
    23401 agaaataatt tcaaaaacaa cttctcctgt ttttggttcg gttggcttat tatcgagaag
    23461 tagctactgg gagtctattt atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt
    23521 attttctttg aaacatcttt cggtactcga cagcatacaa gacaaaagat tgtaaaagac
    23581 cgtaagtgat gacggcaaaa atgactctca cccacagcgt tggtaattct gggaaaagca
    23641 gcaaaaggat catgccaaca tagatgacag cagtcgctac tttgccatac cacttcgctc
    23701 cacttaattt gatattttta gatagcccga ttaatccagc tacaaacata aagccttctt
    23761 ttatcaagaa tagaccaaac aacagccaaa gccacggtcg ataccatgcc atagacaaaa
    23821 tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag caccttccct aagtctgtta
    23881 cctgattata tctccgagca atgaccccgt ctagataatc agacaaacct gagacaacaa
    23941 tgaccgctcc tgctatataa aatgactgtc tcagcacaaa ataaagataa atcggcagca
    24001 aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg tatttcttta cgccagtctg
    24061 tgcttaattt cattttatcg ccctacctcc tcagcactct tttcttttta ttttagctta
    24121 cacggggaat aaaaccaatg atttcactgt ttttattttt ctttacagac actgatatta
    24181 tgagctaatt tgagataatc acttgctgtt ttcatcgttt actcttcttt cttcctgtcg
    24241 tttgcgcatt tcttttacta gtactttatg agcttttttt gatcctcgct ctaggtcacg
    24301 ctgccgttta cgtgttttat aatcgataaa cagactgtct aaatcgtaac ctgtcgggta
    24361 cagatcttct gctgaacgca ccagacgaac acgctgacga ggcacgtgga ttattttttt
    24421 gtctaaatat acatcaatcg tatcttcacc tatatcttca taaactaacg cttctttttg
    24481 gtattcattg acaaatatcc gatcaccttt tgcaaagagc tgacgttctt ttttattctc
    24541 cttcctattt gtaactgctg tcttaggaag aaattctttt ttctgtgtcc gataggcttt
    24601 ttccgccaag taacgctctg cttgatggat caatttcatc gacatatcca ttttatgagc
    24661 aatccacaaa gcttggcttt ctcctgtttc ttccaacccg caactgatat ttaggacgaa
    24721 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
    24781 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
    24841 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
    24901 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
    24961 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
    25021 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
    25081 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
    25141 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
    25201 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
    25261 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
    25321 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
    25381 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
    25441 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
    25501 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
    25561 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
    25621 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
    25681 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
    25741 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
    25801 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
    25861 tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc
    25921 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
    25981 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
    26041 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
    26101 gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc
    26161 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
    26221 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
    26281 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
    26341 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
    26401 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
    26461 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
    26521 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
    26581 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
    26641 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
    26701 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
    26761 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
    26821 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
    26881 gatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
    26941 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
    27001 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
    27061 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
    27121 ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
    27181 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
    27241 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
    27301 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
    27361 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
    27421 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
    27481 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
    27541 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
    27601 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
    27661 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
    27721 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
    27781 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
    27841 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
    27901 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
    27961 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
    28021 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
    28081 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
    28141 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
    28201 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
    28261 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
    28321 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
    28381 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
    28441 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
    28501 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
    28561 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
    28621 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
    28681 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
    28741 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
    28801 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
    28861 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
    28921 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
    28981 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
    29041 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
    29101 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
    29161 gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga
    29221 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
    29281 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
    29341 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
    29401 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
    29461 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
    29521 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
    29581 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
    29641 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
    29701 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
    29761 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
    29821 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
    29881 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
    29941 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
    30001 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
    30061 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
    30121 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
    30181 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
    30241 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
    30301 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
    30361 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
    30421 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
    30481 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
    30541 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
    30601 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
    30661 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
    30721 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
    30781 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
    30841 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
    30901 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
    30961 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
    31021 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
    31081 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
    31141 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
    31201 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
    31261 cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca
    31321 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
    31381 ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca
    31441 atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa
    31501 acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt
    31561 gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc
    31621 gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta
    31681 agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg
    31741 tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact
    31801 tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca
    31861 caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa
    31921 tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc
    31981 tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct
    32041 gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt
    32101 gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac
    32161 tgttcaaacg tccatccttc ttctactgca attgtcagtt ggtcaagaag gtggaacatc
    32221 tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg
    32281 agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt
    32341 ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca
    32401 aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt
    32461 gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt
    32521 tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga
    32581 gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct
    32641 tcccacatct gattgtcggg acgagaatga ttagccagca aaacaagatc atagttctcc
    32701 tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct
    32761 tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca
    32821 ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt
    32881 cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt
    32941 ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg
    33001 tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga
    33061 atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat
    33121 cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc
    33181 gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg
    33241 ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc
    33301 aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg
    33361 ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca
    33421 cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc
    33481 tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga
    33541 gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct
    33601 tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt
    33661 gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac
    33721 tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat
    33781 ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc
    33841 atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact
    33901 gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa
    33961 caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga
    34021 agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc
    34081 ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa
    34141 ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa
    34201 aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat
    34261 cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg
    34321 acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc
    34381 atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg
    34441 cttggcaaat aaagctcttt atatgctttt tcaaaaggtt tcgttacaaa ttcatcctca
    34501 cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct
    34561 gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca
    34621 ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg
    34681 ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct
    34741 agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt
    34801 gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt
    34861 tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag
    34921 ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa
    34981 tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg
    35041 gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt
    35101 tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa
    35161 gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct
    35221 ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg
    35281 agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca
    35341 ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg
    35401 aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct
    35461 gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct
    35521 gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa
    35581 tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc
    35641 taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca
    35701 gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa
    35761 gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc
    35821 aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt
    35881 ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc
    35941 ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc
    36001 atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata
    36061 gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt
    36121 tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct
    36181 ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt
    36241 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt
    36301 ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca
    36361 gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct
    36421 gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag
    36481 caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg
    36541 gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc
    36601 ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa
    36661 atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga
    36721 tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc
    36781 acgacgtata accaaataaa tctactccat ctttgatagc ttccttcatc tgctcaatat
    36841 gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt
    36901 ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt
    36961 gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa
    37021 gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc caattttctg
    37081 gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg
    37141 ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca
    37201 ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag
    37261 ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa
    37321 gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg
    37381 tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat
    37441 ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga
    37501 tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag
    37561 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
    37621 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
    37681 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
    37741 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
    37801 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
    37861 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
    37921 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
    37981 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
    38041 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
    38101 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
    38161 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
    38221 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
    38281 atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
    38341 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta cagaactgta
    38401 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
    38461 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
    38521 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
    38581 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
    38641 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
    38701 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
    38761 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
    38821 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
    38881 tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga
    38941 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
    39001 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
    39061 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaaataat
    39121 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
    39181 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
    39241 aaccgtactt gtataccttg ctagaatcac gggcatccct agaaatcgca aggttatgct
    39301 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
    39361 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
    39421 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
    39481 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
    39541 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
    39601 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
    39661 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
    39721 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
    39781 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
    39841 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
    39901 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
    39961 cattcaatta gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac
    40021 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
    40081 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
    40141 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
    40201 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
    40261 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
    40321 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctcccaaga
    40381 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
    40441 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
    40501 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
    40561 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
    40621 tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa taagcgcttt cagctaaatt
    40681 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
    40741 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
    40801 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
    40861 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
    40921 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
    40981 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
    41041 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
    41101 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
    41161 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
    41221 tgtactgatt tgttgaggaa accttgcgtg ttggatcacc gtttctgttc caacaagaga
    41281 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
    41341 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
    41401 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
    41461 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
    41521 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
    41581 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
    41641 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
    41701 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
    41761 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
    41821 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
    41881 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
    41941 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
    42001 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
    42061 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
    42121 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
    42181 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
    42241 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
    42301 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
    42361 aataactaaa tgcaatataa tctgccgtgt tagcttgcaa gcactgttcg tctccatcta
    42421 acattactgg tgcacagtct tcttccttcc atgtcctgta aatataagct ggataatgcc
    42481 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
    42541 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
    42601 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
    42661 acatatgatg acaggcttga taaatcactt tttttgttag tgtagcaatg ggttcaagtt
    42721 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
    42781 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
    42841 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
    42901 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
    42961 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
    43021 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
    43081 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
    43141 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
    43201 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
    43261 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
    43321 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
    43381 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
    43441 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
    43501 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
    43561 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
    43621 ataaccctta aagcttcacg tggaatagtg aacgtctgct tttcactgga catattacaa
    43681 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
    43741 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
    43801 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
    43861 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
    43921 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcctt gattgattcc
    43981 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
    44041 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
    44101 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
    44161 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
    44221 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
    44281 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
    44341 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
    44401 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
    44461 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
    44521 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccccca
    44581 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
    44641 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
    44701 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
    44761 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
    44821 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
    44881 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
    44941 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
    45001 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
    45061 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
    45121 tgataaacaa ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat
    45181 gtttctattt tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt
    45241 caaatagcta ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa
    45301 ttccctcaac tgttttttga aaggattgat ttatgcaggg aagaatgctg atttcacaac
    45361 ttctttataa acggtgttca ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat
    45421 ataagaaact attttttcgt ttttttctct aatactcagg ccagaacgct gatttcacaa
    45481 cctctttatt tcacattcaa tatccacatg aatcccacaa atacaaagaa cagaccatac
    45541 atgattggag atacttcttt tcctctttta gctgctaaca tggtaattgg atagaagata
    45601 aaacctaatg caatcccatc tgaaatacta taagtcaaag gcatgccgat caagatcaag
    45661 aatgaaggga tcgcaatctc taagtttccc cattcgattt gtcgcaaaga ttgtgccatc
    45721 aatactccta ccataatcaa tgctggtgca gttacttgtg aagtaacaac tgatagtaat
    45781 ggagaaaaga acaagcctaa caagaagaaa atccctgtag taatagctgt gatacctgaa
    45841 cgtcctccaa cggcgatacc agctgatgat tctacaaatg caccaacagg agaagtacca
    45901 aataaggaac ccgcaagcat agctgttgaa tcagaagcta aagctttccc tacacgaggc
    45961 atcttgttgt ctttcatgaa acctgcttgg tttgctaatc caactaaagt tcctgctgta
    46021 tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc acatttgaag agaattgatg
    46081 tcccctacat gttttaatgc aactaaaaat gttggtttca aacttggtgc tgcggcaaca
    46141 atttcagaag gcattggaat caaacctgtg accagaccta aaatcgttgt agctgccatc
    46201 ccgatgaaga tgccacctgg tacacgccga acaagcaaga tagcagttac gactaatcca
    46261 aaaatcgtca accaagtaga accaacgttg agtgatccta atcctactaa tgtagattca
    46321 ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag cgataaatag accgataccg
    46381 ccagaaatcg catatttcaa atctgctggg atcgcatcaa tgatcatttc ccgcagcttg
    46441 aagattgtga tcaaaataaa aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc
    46501 catggaatcc ccattcctaa acaaacagaa taagcaaaaa aagcattgat cccaagagca
    46561 ggtgccgttc caattggata acgggctaat acccccatta aaacacagcc gatcgcactg
    46621 gctaatgcag tcgcagtaaa cactgctcct tcatccattc cagaagcacc taatacagtt
    46681 ggatttacaa ataagatata agccattgag ataaatgttg taaatcctgc cagtacctca
    46741 cgttttaccg tggtgttcaa tttttctatt tcgaagtagg aaagtatttt ttccttcatc
    46801 ttccatcccc ctaaatccga acattataaa taaagtccgt taaaatttcg atttattaat
    46861 aaaacaacgt gttttatcat atactaaaac aaaacataag taaacaaaaa attcggattt
    46921 taagcaaaat ccgaacgttc catctgatta aatcccttta tctcaaagtt catgaacatt
    46981 cactttggat tacaagaatt tctttattgt gttaataaag tttcttcatt ctagtctcaa
    47041 aaatcaatag tgtgatttga ttttaccatt tcgtttctgt tcttctttga tgaattcttt
    47101 tgtatcgaca ggattcagat tatttagtcc gccaaaacct gtatcgacca tttttcccca
    47161 gccttttgtc ttcttttttc ctgatttgag attctttgct cgaagcttct cactatgttt
    47221 tgctcgccga tacttcaaat aaccttctaa tcttcccatt agatccactg tttttcctcc
    47281 tttttattca atcagacgac tcagcttttc ctataacaaa ataattgcct tcagggtcag
    47341 aaaaattgaa agtcaagatg ccattgttat ctgtgatttc tcctggttct tccatgttct
    47401 cgtagatagt ttcaaattca tccgtaaaaa atactaaaga aggcggtggt cctaatacct
    47461 ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc taattctaat tcttttgaga
    47521 tgctgagtac aatactctta aattcttctg gcaattcgat tgtttcagaa atttctgcac
    47581 caattttttc tacaaagaat cggcaaatca tctctacgtc attgacataa agcatcacac
    47641 gtgttttttg tatcatcatt tgacccttct ttctatttga ttttctttcc tgtctataaa
    47701 ctgattccat tatatcacga aagcgcaaac accttgtgtt tgatactatt caagaaaaat
    47761 aaagcaatag tttctaggat tgctttcctc cacaaacttt ttttacaaaa aaaaaacggc
    47821 aatgaatgcc gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg
    47881 ttagtgtgtg tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt
    47941 cgattgcctg tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg
    48001 ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc acgccagttg agacagcgac
    48061 agatcgctcc ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat
    48121 ctgcacctaa aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg
    48181 cgatcttttc gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat
    48241 agatatctac aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta
    48301 aagcttcaat agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc
    48361 actgagcaat gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt
    48421 ttgctgcctc attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat
    48481 cgataccatt tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg
    48541 tatccactat tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa
    48601 tagatagtct gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa
    48661 tcctttttta cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat
    48721 ggctgttttt tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat
    48781 accttgatgt ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc
    48841 catttcctca gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc
    48901 gatactcact aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag
    48961 tgcataagct tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt
    49021 atctgtaaat gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt
    49081 tcgaatttcg tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt
    49141 gacaaaattc caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg
    49201 ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg
    49261 aatgatgaaa ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt
    49321 attttcttta tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg
    49381 ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg
    49441 attttcgcaa atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa
    49501 ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc
    49561 agccatttca tcccaccttt attttgtttg taagactagt tcacctcttt ttgttgcaaa
    49621 tgcaactatt ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt
    49681 ttcttctttt acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt
    49741 aactgtttct tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat
    49801 cccatgttgg aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa
    49861 gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca
    49921 aaaactgctc gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta
    49981 cgcacatttt cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct
    50041 ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca
    50101 tctacttttg gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa
    50161 gtttcttttc ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc
    50221 tctttatcaa atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt
    50281 ttgtattgag ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta
    50341 tgtaatcaag tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat
    50401 aaataaaaga atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga
    50461 ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga
    50521 cgagctattc ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc
    50581 cccactaaac ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca
    50641 tgagaagctg tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga
    50701 cctctcggta aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact
    50761 tattgtcatt gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat
    50821 agtctgttac atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg
    50881 cgatgatctt tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca
    50941 atacgtcacc atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat
    51001 cacgtttgta ccgacgttgc cataacactt caaagtctgc tgttaggcga atcgtgatca
    51061 cctgatatcc gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag
    51121 gatactcgga cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat
    51181 agaaaggata aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga
    51241 tggattctgc atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc
    51301 gttcccgtag tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa
    51361 tgaaatactt tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac
    51421 agtccaatct tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg
    51481 cgaagagact tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg
    51541 actaaaagct ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa
    51601 tcttcttctt tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg
    51661 atttctttca gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta
    51721 ccgatagcaa tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc
    51781 ggtcttgtat ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt
    51841 gttccaagtt ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt
    51901 gtcttcagga tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat
    51961 ccgctgtcga ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact
    52021 gcatcaacca tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg
    52081 atatatttag cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct
    52141 gcgcctagca tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa
    52201 ttcaactctt ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac
    52261 cttatctttt tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc
    52321 cattttgccc atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg
    52381 cttctggtta tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc
    52441 caaactcttt tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag
    52501 tccaacttgt tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg
    52561 tttcaattgt gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc
    52621 ttcttttgcc agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat
    52681 tttccctact ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag
    52741 tgctgcggct actgtatcta catcggttgc tgtagcattg ttgaatccca tcgaattaaa
    52801 gatcgttact agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat
    52861 gatcgctcct cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa
    52921 gaaattggtt agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat
    52981 ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata
    53041 aggaaagaaa actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat
    53101 ccctcgaaat gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata
    53161 cacacccact acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc
    53221 tgataattca gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac
    53281 catcacgacc atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt
    53341 gatatcttcg atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc
    53401 ttcaaataag tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat
    53461 tttacgaatg atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa
    53521 accaccgacc aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt
    53581 catcgttgcc atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa
    53641 acgagccaaa ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc
    53701 taaacccaaa tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg
    53761 catatgaaat ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct
    53821 cgaaccggca ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg
    53881 tcctgctgat ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga
    53941 tgcctgagtc aaaatatttt cgatgataaa attgataacg cccatgtt
//
LOCUS       DAEGIQ010000006        53536 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000006 DAEGIQ010000000
VERSION     DAEGIQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.006"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..478)
                     /locus_tag="KMT45_000308"
     CDS             complement(<1..478)
                     /locus_tag="KMT45_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HBE7003798.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHYFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIG"
     gene            1124..3931
                     /locus_tag="KMT45_000309"
     CDS             1124..3931
                     /locus_tag="KMT45_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBE7003799.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            4122..4613
                     /locus_tag="KMT45_000310"
     CDS             4122..4613
                     /locus_tag="KMT45_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7003800.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            4716..5705
                     /gene="manX"
                     /locus_tag="KMT45_000311"
     CDS             4716..5705
                     /gene="manX"
                     /locus_tag="KMT45_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBE7003801.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5738..6541
                     /locus_tag="KMT45_000312"
     CDS             5738..6541
                     /locus_tag="KMT45_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBE7003802.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            6562..7476
                     /locus_tag="KMT45_000313"
     CDS             6562..7476
                     /locus_tag="KMT45_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBE7003803.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            7559..7933
                     /locus_tag="KMT45_000314"
     CDS             7559..7933
                     /locus_tag="KMT45_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBE7003804.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8072..8317
                     /locus_tag="KMT45_000315"
     CDS             8072..8317
                     /locus_tag="KMT45_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBE7003805.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8489..9553
                     /locus_tag="KMT45_000316"
     CDS             8489..9553
                     /locus_tag="KMT45_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBE7003806.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9799..10224
                     /locus_tag="KMT45_000317"
     CDS             9799..10224
                     /locus_tag="KMT45_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBE7003807.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10217..10666
                     /gene="nusB"
                     /locus_tag="KMT45_000318"
     CDS             10217..10666
                     /gene="nusB"
                     /locus_tag="KMT45_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBE7003808.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10763..11617
                     /locus_tag="KMT45_000319"
     CDS             10763..11617
                     /locus_tag="KMT45_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBE7003809.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            11618..12955
                     /locus_tag="KMT45_000320"
     CDS             11618..12955
                     /locus_tag="KMT45_000320"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBE7003810.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12960..13184
                     /locus_tag="KMT45_000321"
     CDS             12960..13184
                     /locus_tag="KMT45_000321"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBE7003811.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            13186..14076
                     /locus_tag="KMT45_000322"
     CDS             13186..14076
                     /locus_tag="KMT45_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBE7003812.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            14085..14903
                     /locus_tag="KMT45_000323"
     CDS             14085..14903
                     /locus_tag="KMT45_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBE7003813.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            15010..15459
                     /locus_tag="KMT45_000324"
     CDS             15010..15459
                     /locus_tag="KMT45_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBE7003814.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            15477..17159
                     /gene="recN"
                     /locus_tag="KMT45_000325"
     CDS             15477..17159
                     /gene="recN"
                     /locus_tag="KMT45_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBE7003815.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(17214..17330)
                     /locus_tag="KMT45_000326"
     CDS             complement(17214..17330)
                     /locus_tag="KMT45_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBE7003816.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(17403..18359)
                     /locus_tag="KMT45_000327"
     CDS             complement(17403..18359)
                     /locus_tag="KMT45_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBE7003817.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            18530..18898
                     /locus_tag="KMT45_000328"
     CDS             18530..18898
                     /locus_tag="KMT45_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBE7003818.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            19068..19499
                     /gene="mraZ"
                     /locus_tag="KMT45_000329"
     CDS             19068..19499
                     /gene="mraZ"
                     /locus_tag="KMT45_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBE7003819.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            19520..20479
                     /gene="rsmH"
                     /locus_tag="KMT45_000330"
     CDS             19520..20479
                     /gene="rsmH"
                     /locus_tag="KMT45_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBE7003820.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLRQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            20484..20885
                     /gene="ftsL"
                     /locus_tag="KMT45_000331"
     CDS             20484..20885
                     /gene="ftsL"
                     /locus_tag="KMT45_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBE7003821.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20886..23078
                     /locus_tag="KMT45_000332"
     CDS             20886..23078
                     /locus_tag="KMT45_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBE7003822.1"
                     /translation="MSLKNKIWRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            23104..24066
                     /locus_tag="KMT45_000333"
     CDS             23104..24066
                     /locus_tag="KMT45_000333"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBE7003823.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            24080..25441
                     /locus_tag="KMT45_000334"
     CDS             24080..25441
                     /locus_tag="KMT45_000334"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBE7003824.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            25467..26555
                     /gene="murG"
                     /locus_tag="KMT45_000335"
     CDS             25467..26555
                     /gene="murG"
                     /locus_tag="KMT45_000335"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBE7003825.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            26570..27790
                     /locus_tag="KMT45_000336"
     CDS             26570..27790
                     /locus_tag="KMT45_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBE7003826.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27946..29271
                     /gene="ftsA"
                     /locus_tag="KMT45_000337"
     CDS             27946..29271
                     /gene="ftsA"
                     /locus_tag="KMT45_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBE7003827.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            29294..30535
                     /gene="ftsZ"
                     /locus_tag="KMT45_000338"
     CDS             29294..30535
                     /gene="ftsZ"
                     /locus_tag="KMT45_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBE7003828.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            30552..31229
                     /locus_tag="KMT45_000339"
     CDS             30552..31229
                     /locus_tag="KMT45_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBE7003829.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            31243..31842
                     /locus_tag="KMT45_000340"
     CDS             31243..31842
                     /locus_tag="KMT45_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBE7003830.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31857..32144
                     /locus_tag="KMT45_000341"
     CDS             31857..32144
                     /locus_tag="KMT45_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBE7003831.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            32193..32975
                     /locus_tag="KMT45_000342"
     CDS             32193..32975
                     /locus_tag="KMT45_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBE7003832.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32996..33703
                     /locus_tag="KMT45_000343"
     CDS             32996..33703
                     /locus_tag="KMT45_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBE7003833.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33965..36763
                     /gene="ileS"
                     /locus_tag="KMT45_000344"
     CDS             33965..36763
                     /gene="ileS"
                     /locus_tag="KMT45_000344"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBE7003834.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36812..38338)
                     /locus_tag="KMT45_000345"
     CDS             complement(36812..38338)
                     /locus_tag="KMT45_000345"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBE7003835.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(38353..39000)
                     /locus_tag="KMT45_000346"
     CDS             complement(38353..39000)
                     /locus_tag="KMT45_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBE7003836.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(39184..39513)
                     /locus_tag="KMT45_000347"
     CDS             complement(39184..39513)
                     /locus_tag="KMT45_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBE7003837.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            39690..40418
                     /gene="sfsA"
                     /locus_tag="KMT45_000348"
     CDS             39690..40418
                     /gene="sfsA"
                     /locus_tag="KMT45_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBE7003838.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            40434..41447
                     /locus_tag="KMT45_000349"
     CDS             40434..41447
                     /locus_tag="KMT45_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE7003839.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFYVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            41447..42724
                     /locus_tag="KMT45_000350"
     CDS             41447..42724
                     /locus_tag="KMT45_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBE7003840.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKKKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42787..45489
                     /locus_tag="KMT45_000351"
     CDS             42787..45489
                     /locus_tag="KMT45_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBE7003841.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKKEFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            45641..45958
                     /locus_tag="KMT45_000352"
     CDS             45641..45958
                     /locus_tag="KMT45_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7003842.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45988..46308
                     /locus_tag="KMT45_000353"
     CDS             45988..46308
                     /locus_tag="KMT45_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBE7003843.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            46416..47876
                     /locus_tag="KMT45_000354"
     CDS             46416..47876
                     /locus_tag="KMT45_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7003844.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47944..48165
                     /locus_tag="KMT45_000355"
     CDS             47944..48165
                     /locus_tag="KMT45_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003845.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            48196..48378
                     /locus_tag="KMT45_000356"
     CDS             48196..48378
                     /locus_tag="KMT45_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBE7003846.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            48378..48791
                     /locus_tag="KMT45_000357"
     CDS             48378..48791
                     /locus_tag="KMT45_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBE7003847.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48914..50095
                     /locus_tag="KMT45_000358"
     CDS             48914..50095
                     /locus_tag="KMT45_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7003848.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(50166..50330)
                     /locus_tag="KMT45_000359"
     CDS             complement(50166..50330)
                     /locus_tag="KMT45_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003849.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            50484..50557
                     /locus_tag="KMT45_000360"
     tRNA            50484..50557
                     /locus_tag="KMT45_000360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50518..50520,aa:Arg,seq:cct)
     gene            complement(50626..51765)
                     /locus_tag="KMT45_000361"
     CDS             complement(50626..51765)
                     /locus_tag="KMT45_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE7003850.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(52064..52699)
                     /locus_tag="KMT45_000362"
     CDS             complement(52064..52699)
                     /locus_tag="KMT45_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003851.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52812..53447)
                     /locus_tag="KMT45_000363"
     CDS             complement(52812..53447)
                     /locus_tag="KMT45_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBE7003852.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT        17677 a         9044 c        11308 g        15507 t
ORIGIN      
        1 tgcctatcat tttttgttga gctacaatta aacgtttatt agttaatgcg tatgcataat
       61 tattgtcatg cttagttgat gagatataat tatgcaatcc tacaaagcag aagattacgt
      121 attcgtcact gttaagttga tctgatatta agttaaaata atgagttgtc cattttttag
      181 tcattccttt accgtagccg tatttaagac aaaattctac catctcttca gtggttctca
      241 ttctatcatt acctatatca tgattgtttt gtgcagtatg atcagatatg tttaattttc
      301 ttttttctga tggatcctta tctaaatctt ctaaattcaa agatcttatt attttataag
      361 gagtagtcat accgtaacca gcttttttca tacaattgcc gcaaacaatt tgtttatctt
      421 tgattttata tgaagttaag ccaattttac ctccgcactt tgcacattgt ttactcacat
      481 tagttcctcc tagatatcag ctttctacta ctattaacat tctatcaata gtgaccatac
      541 tttgaccata ctttttagaa agaatagaaa attccaagat atttaataat agaagaaaag
      601 cactaacatc atgggttcag aagaattgag agcttatcag gaaacagtat aattgaagtg
      661 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
      721 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
      781 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
      841 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
      901 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
      961 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     1021 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     1081 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     1141 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     1201 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     1261 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     1321 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     1381 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     1441 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     1501 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     1561 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     1621 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     1681 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     1741 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     1801 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     1861 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     1921 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     1981 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     2041 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     2101 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     2161 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     2221 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     2281 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     2341 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     2401 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     2461 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     2521 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     2581 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     2641 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     2701 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     2761 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     2821 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     2881 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     2941 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     3001 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     3061 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     3121 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     3181 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     3241 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     3301 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     3361 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     3421 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     3481 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     3541 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     3601 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     3661 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     3721 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     3781 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     3841 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     3901 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     3961 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     4021 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     4081 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     4141 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     4201 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     4261 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     4321 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     4381 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     4441 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     4501 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     4561 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     4621 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     4681 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
     4741 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
     4801 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
     4861 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
     4921 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
     4981 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
     5041 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
     5101 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
     5161 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
     5221 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
     5281 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
     5341 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
     5401 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
     5461 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
     5521 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
     5581 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
     5641 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
     5701 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
     5761 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
     5821 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
     5881 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
     5941 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
     6001 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
     6061 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
     6121 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
     6181 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
     6241 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
     6301 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
     6361 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
     6421 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
     6481 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
     6541 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
     6601 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
     6661 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
     6721 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
     6781 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
     6841 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
     6901 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
     6961 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
     7021 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
     7081 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
     7141 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
     7201 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
     7261 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
     7321 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
     7381 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
     7441 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
     7501 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
     7561 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
     7621 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
     7681 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
     7741 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
     7801 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
     7861 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
     7921 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
     7981 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
     8041 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
     8101 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
     8161 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
     8221 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
     8281 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
     8341 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
     8401 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
     8461 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
     8521 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
     8581 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
     8641 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
     8701 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
     8761 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
     8821 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
     8881 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
     8941 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
     9001 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
     9061 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
     9121 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
     9181 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
     9241 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
     9301 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
     9361 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
     9421 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
     9481 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
     9541 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
     9601 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
     9661 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
     9721 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
     9781 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
     9841 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
     9901 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
     9961 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    10021 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    10081 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    10141 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    10201 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    10261 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    10321 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    10381 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    10441 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    10501 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    10561 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    10621 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    10681 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    10741 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    10801 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    10861 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    10921 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    10981 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    11041 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    11101 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    11161 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    11221 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    11281 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    11341 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    11401 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    11461 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    11521 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    11581 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    11641 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    11701 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    11761 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    11821 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    11881 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    11941 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    12001 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    12061 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    12121 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    12181 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    12241 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    12301 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    12361 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    12421 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    12481 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    12541 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    12601 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    12661 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    12721 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    12781 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    12841 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    12901 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    12961 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    13021 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    13081 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    13141 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    13201 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    13261 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    13321 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    13381 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    13441 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    13501 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    13561 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    13621 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    13681 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    13741 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    13801 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    13861 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    13921 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    13981 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    14041 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    14101 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    14161 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    14221 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    14281 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    14341 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    14401 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    14461 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    14521 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    14581 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    14641 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    14701 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    14761 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    14821 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    14881 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    14941 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    15001 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    15061 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    15121 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    15181 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    15241 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    15301 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    15361 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    15421 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    15481 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    15541 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    15601 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    15661 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    15721 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    15781 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    15841 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    15901 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    15961 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    16021 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    16081 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    16141 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    16201 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    16261 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    16321 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    16381 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    16441 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    16501 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    16561 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    16621 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    16681 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    16741 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    16801 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    16861 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    16921 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    16981 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    17041 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    17101 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    17161 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    17221 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    17281 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    17341 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    17401 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    17461 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    17521 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    17581 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    17641 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    17701 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    17761 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    17821 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    17881 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    17941 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    18001 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    18061 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    18121 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    18181 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    18241 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    18301 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    18361 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    18421 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    18481 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    18541 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    18601 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    18661 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    18721 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    18781 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    18841 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    18901 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    18961 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    19021 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    19081 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    19141 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    19201 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    19261 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    19321 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    19381 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    19441 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    19501 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    19561 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    19621 tgccggacat agtgaatatt tattaagaca actaagttct aaagggcatc tgtatgcgtt
    19681 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    19741 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    19801 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    19861 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    19921 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    19981 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    20041 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    20101 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    20161 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    20221 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    20281 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    20341 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    20401 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    20461 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    20521 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    20581 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    20641 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    20701 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    20761 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    20821 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    20881 agtaaatgag tcttaaaaat aaaatttggc ggtttatgga aaagaaaaat ctaaatccga
    20941 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    21001 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    21061 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    21121 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    21181 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    21241 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    21301 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    21361 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    21421 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    21481 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    21541 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    21601 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    21661 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    21721 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    21781 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    21841 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    21901 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    21961 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    22021 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    22081 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    22141 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    22201 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    22261 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    22321 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    22381 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    22441 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaccggaa
    22501 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    22561 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    22621 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    22681 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    22741 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    22801 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    22861 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    22921 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    22981 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    23041 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    23101 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    23161 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    23221 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    23281 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    23341 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    23401 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    23461 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    23521 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    23581 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    23641 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    23701 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    23761 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    23821 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    23881 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    23941 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    24001 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    24061 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    24121 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    24181 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    24241 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    24301 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    24361 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    24421 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    24481 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    24541 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    24601 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    24661 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    24721 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    24781 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    24841 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    24901 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    24961 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    25021 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    25081 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtttt
    25141 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    25201 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    25261 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    25321 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    25381 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    25441 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    25501 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    25561 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    25621 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    25681 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    25741 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    25801 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    25861 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    25921 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    25981 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    26041 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    26101 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    26161 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    26221 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    26281 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    26341 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    26401 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    26461 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    26521 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    26581 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    26641 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    26701 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    26761 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    26821 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    26881 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    26941 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    27001 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    27061 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    27121 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    27181 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    27241 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    27301 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    27361 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    27421 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    27481 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    27541 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    27601 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    27661 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    27721 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    27781 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    27841 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    27901 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    27961 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    28021 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    28081 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    28141 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    28201 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    28261 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    28321 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    28381 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    28441 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    28501 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    28561 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    28621 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    28681 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    28741 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    28801 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    28861 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    28921 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    28981 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    29041 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    29101 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    29161 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    29221 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    29281 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    29341 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    29401 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    29461 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    29521 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    29581 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    29641 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    29701 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    29761 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    29821 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    29881 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    29941 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    30001 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    30061 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    30121 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    30181 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    30241 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    30301 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    30361 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    30421 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    30481 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    30541 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    30601 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    30661 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    30721 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    30781 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    30841 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    30901 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    30961 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    31021 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    31081 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    31141 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    31201 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    31261 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    31321 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    31381 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    31441 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    31501 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    31561 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    31621 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    31681 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    31741 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    31801 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    31861 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    31921 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    31981 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    32041 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    32101 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    32161 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    32221 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    32281 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    32341 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    32401 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    32461 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    32521 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    32581 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    32641 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    32701 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    32761 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    32821 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    32881 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    32941 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    33001 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    33061 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    33121 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    33181 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    33241 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    33301 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    33361 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    33421 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    33481 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    33541 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    33601 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    33661 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    33721 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    33781 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    33841 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    33901 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    33961 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    34021 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    34081 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    34141 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    34201 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    34261 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    34321 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    34381 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    34441 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaagctata tttacaaagg
    34501 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    34561 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    34621 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    34681 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    34741 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    34801 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    34861 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    34921 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    34981 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    35041 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    35101 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    35161 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    35221 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    35281 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    35341 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    35401 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    35461 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    35521 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    35581 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    35641 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    35701 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    35761 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    35821 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    35881 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    35941 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    36001 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    36061 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    36121 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    36181 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    36241 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    36301 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    36361 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    36421 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    36481 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    36541 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    36601 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    36661 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    36721 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    36781 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    36841 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    36901 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    36961 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    37021 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    37081 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    37141 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    37201 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    37261 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    37321 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    37381 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    37441 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    37501 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    37561 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    37621 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    37681 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    37741 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    37801 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    37861 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    37921 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    37981 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    38041 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    38101 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    38161 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    38221 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    38281 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    38341 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    38401 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    38461 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    38521 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    38581 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    38641 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    38701 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    38761 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    38821 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    38881 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    38941 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    39001 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    39061 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    39121 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    39181 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    39241 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    39301 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    39361 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    39421 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    39481 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat caccccttta
    39541 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    39601 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    39661 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    39721 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    39781 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    39841 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    39901 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    39961 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    40021 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    40081 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    40141 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    40201 atgccgctta tgtcttgttt attgctcaat tcgaacatct tcatctggca acgatccatg
    40261 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    40321 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    40381 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    40441 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    40501 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    40561 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    40621 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    40681 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    40741 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    40801 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    40861 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccag gtattagatg
    40921 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    40981 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    41041 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    41101 tttatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    41161 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    41221 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    41281 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    41341 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    41401 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    41461 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    41521 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    41581 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    41641 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    41701 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    41761 agccaacgtc aaacgtcaga ttgagggttt gaagaaaaaa ccagaaatct tgattggtac
    41821 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    41881 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    41941 aattcttcat caagctccaa cagagtatca gctagccttt ttttcagcga cagcagatcg
    42001 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    42061 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    42121 agatgactat ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    42181 tcaactgact gaattaggcg tgatggaaga aaaattgctg tatagaggtg taccggtagg
    42241 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    42301 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    42361 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    42421 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    42481 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    42541 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    42601 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    42661 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    42721 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    42781 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    42841 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    42901 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    42961 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    43021 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    43081 gctgcgattc tttatccacc gcgggggctg catacgatta tttttggaga aactggtaca
    43141 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    43201 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    43261 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    43321 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    43381 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    43441 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    43501 gaaagtttct tatctacgtt taatcgacgg attcctatgg caatcacctt gccgaatctt
    43561 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    43621 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    43681 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    43741 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    43801 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    43861 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    43921 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    43981 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    44041 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    44101 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    44161 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    44221 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    44281 aaggaatttc aagtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    44341 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    44401 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    44461 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    44521 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    44581 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    44641 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    44701 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    44761 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    44821 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    44881 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    44941 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    45001 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    45061 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    45121 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    45181 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    45241 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    45301 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    45361 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    45421 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    45481 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    45541 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    45601 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    45661 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    45721 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    45781 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    45841 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    45901 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    45961 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    46021 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    46081 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    46141 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    46201 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    46261 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    46321 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    46381 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    46441 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    46501 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    46561 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    46621 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    46681 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    46741 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    46801 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    46861 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    46921 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    46981 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    47041 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    47101 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    47161 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    47221 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    47281 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    47341 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    47401 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    47461 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    47521 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    47581 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    47641 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    47701 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    47761 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    47821 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    47881 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    47941 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    48001 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    48061 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    48121 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    48181 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    48241 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    48301 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    48361 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    48421 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    48481 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    48541 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    48601 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    48661 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    48721 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    48781 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    48841 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    48901 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    48961 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    49021 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    49081 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    49141 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    49201 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    49261 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    49321 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    49381 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    49441 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    49501 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    49561 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    49621 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    49681 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    49741 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    49801 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    49861 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    49921 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    49981 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    50041 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    50101 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    50161 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    50221 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    50281 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    50341 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    50401 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    50461 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    50521 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    50581 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    50641 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    50701 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    50761 gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg
    50821 caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac
    50881 ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt
    50941 gatttcaaaa ttctcgaata attctttttg attgatttta aactttttta agagatcaat
    51001 cgtatgttta tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt ttccccattc
    51061 atttaattta taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc
    51121 ttcccatgtt aatcctagaa gttcagcaaa tcgcattcca gtggcaccag caactaacac
    51181 gagcatagga gaagagtact gcgcagaaag gttttcttct acgagtttca ttaaagattt
    51241 aaattgatca taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat
    51301 tactgctttt ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga
    51361 cgctctaata tggttattaa acttaataac ggtagatttt acatggtctt tagcatactt
    51421 atttaaaaag cgttgatatc ccactctatc taaatcagaa atcaataccg ctggcatata
    51481 tttctttatg ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc
    51541 ttctttataa agttgcatcc aatcctcaaa gtaatctgat aataatagat ttttgcgatc
    51601 ccctttaagg cctttgttca agttatattc taattcgttg gcggcatctt ttgcttctcc
    51661 ttttgtctta aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga
    51721 aatttcgtat tgccaactat ttccacgttt gactaatctt gccataattg aatcagctct
    51781 ctttctctga tacaatagga actataaaga agcccattgt ttgggtttta ttattttttg
    51841 cacatccttt ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat ggtataattg
    51901 gatgtgtatt agatcatcca atcagaggtg tttattatga ttaaagtgat ttttaaaaga
    51961 ataactgaac ttccagagtt tagattattt gttgattatt ggtatgtctg gttttttatt
    52021 gttgcattgc tgataggtct agatgtcata ttagaaagaa aacttaagta agagttgagt
    52081 taataaattg aattcctaaa tgagatattt tgtagcccat attttgtttg ccgacatcac
    52141 ctaatactat ggagctaatc gttttttcta acagaccaaa atcagataat ctatttaagc
    52201 taccatctat tagctcttgt gaatagccag acatgctgat atttccagtg aaattttcgt
    52261 ttccacgact cattaagcat gctaacaatt gaatctcaat cgatgttaat tgattcagta
    52321 caagaagata atactcctcg ttatcccatg aagcgttgtt aattcgaagt aaacaattct
    52381 tataaagatt tttatacata tctattttat tttgtgcctt tgatttttct acctcgtcat
    52441 ttatcccttc gaatattcca atgagttggt ccttgttctc taagttgaaa acatcttctg
    52501 gaatttgatc gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat ttctcgatgc
    52561 gtttgaaacg cttctcgttt ttagacccaa aataaagtga ctgcacaacg ccaccaatat
    52621 aaggaattgc tccaataccg ccttcaacta ttaattcaag atattctttt ttggttagtt
    52681 tttcattgct attatccata gttacctcct attttgtcat ttcctttaca agcattgtga
    52741 ctcctgatgg cactcttttg tataggtttt attattgtgc acaatctaac tcttagtggg
    52801 gcgtggttca tttatttttt acttacttta ctattaatca tatcccattg agcataggat
    52861 ttttctatgt tcgatcctct tgaactaatt tctcctgtaa aagttacttt atcgcccttt
    52921 gtaaaggtat cagcattgtt taagtccttc gcaaaaatta catatgaaac tttaggtgtt
    52981 gcagaaatat cattccaact tttatcatcg tactctttgt ctgctataat ggctattcta
    53041 gtagtcattg attcgataac agttccttca aaagtatatg ttttaccact aatatattta
    53101 gtaaaagctg tattttgctc agtagctgga gtaatagaat aataactatc taagaaagca
    53161 ccccagtcat gattatttgt ctctaaaaat tcttcaaatt gagttaggct tttattgctg
    53221 ctgttagatg tagtagtttg agtatcagat gatgagcttt cctgtttgtt attgtcattt
    53281 gcctcgtttg atgaggaaag cgagaaacct acggtagtaa aaataatccc gataataagg
    53341 agaatcatag gtgttttctt atttgttttt ttgataaatc ttacaattaa catgattaaa
    53401 cctataataa ataaaacaat accagtaaaa ccaaatatac cgtccattaa tagatcctct
    53461 ttctcttttg atattgaata ttataaacca agcaattctt ttttcttaaa atcgaattct
    53521 tcttgattaa taatcc
//
LOCUS       DAEGIQ010000007        53309 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000007 DAEGIQ010000000
VERSION     DAEGIQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.007"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            97..2331
                     /gene="pcrA"
                     /locus_tag="KMT45_000364"
     CDS             97..2331
                     /gene="pcrA"
                     /locus_tag="KMT45_000364"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBE7003853.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIQYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNNWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2348..4384
                     /gene="ligA"
                     /locus_tag="KMT45_000365"
     CDS             2348..4384
                     /gene="ligA"
                     /locus_tag="KMT45_000365"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBE7003854.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            4490..4795
                     /gene="gatC"
                     /locus_tag="KMT45_000366"
     CDS             4490..4795
                     /gene="gatC"
                     /locus_tag="KMT45_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBE7003855.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            4795..6267
                     /gene="gatA"
                     /locus_tag="KMT45_000367"
     CDS             4795..6267
                     /gene="gatA"
                     /locus_tag="KMT45_000367"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBE7003856.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHTQQPAILGGKDE"
     gene            6264..7694
                     /gene="gatB"
                     /locus_tag="KMT45_000368"
     CDS             6264..7694
                     /gene="gatB"
                     /locus_tag="KMT45_000368"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBE7003857.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            7704..8759
                     /locus_tag="KMT45_000369"
     CDS             7704..8759
                     /locus_tag="KMT45_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBE7003858.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEARRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRVIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            8870..10243
                     /gene="rlmD"
                     /locus_tag="KMT45_000370"
     CDS             8870..10243
                     /gene="rlmD"
                     /locus_tag="KMT45_000370"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBE7003859.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRTGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNSLNNKIENVHYEVGKAEKVMKKWQEKGVKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLLAEAGYVVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            10246..10374
                     /locus_tag="KMT45_000371"
     CDS             10246..10374
                     /locus_tag="KMT45_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBE7003860.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            10799..11002
                     /locus_tag="KMT45_000372"
     CDS             10799..11002
                     /locus_tag="KMT45_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBE7003861.1"
                     /translation="MKNGELEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENV
                     LFEEQWKKFLLSKALQKKFGENH"
     gene            11080..12093
                     /locus_tag="KMT45_000373"
     CDS             11080..12093
                     /locus_tag="KMT45_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE7003862.1"
                     /translation="MKKKILKFIGWFFGIILGITFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(12161..13195)
                     /locus_tag="KMT45_000374"
     CDS             complement(12161..13195)
                     /locus_tag="KMT45_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7003863.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQATRQRIVEAAAA
                     LNYDISTRKYIKKRMPSIGVISTIGKASEEQDVYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYRLGGWTEKQGEVLATDLLENESPLPTAILVGSDPLAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPSLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     DSFIPRTKRY"
     gene            13309..15534
                     /locus_tag="KMT45_000375"
                     /pseudo
     CDS             13309..15534
                     /locus_tag="KMT45_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
     gene            15552..17435
                     /locus_tag="KMT45_000376"
     CDS             15552..17435
                     /locus_tag="KMT45_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBE7003864.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGTWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEVNIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            17416..18390
                     /locus_tag="KMT45_000377"
     CDS             17416..18390
                     /locus_tag="KMT45_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBE7003865.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            19048..20070
                     /gene="aroF"
                     /locus_tag="KMT45_000378"
     CDS             19048..20070
                     /gene="aroF"
                     /locus_tag="KMT45_000378"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBE7003866.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            20103..21167
                     /gene="aroB"
                     /locus_tag="KMT45_000379"
     CDS             20103..21167
                     /gene="aroB"
                     /locus_tag="KMT45_000379"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBE7003867.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKNLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            21169..22335
                     /gene="aroC"
                     /locus_tag="KMT45_000380"
     CDS             21169..22335
                     /gene="aroC"
                     /locus_tag="KMT45_000380"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBE7003868.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKVAVKEYRDYLKHY"
     gene            22352..23443
                     /locus_tag="KMT45_000381"
     CDS             22352..23443
                     /locus_tag="KMT45_000381"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBE7003869.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAI
                     EQQIVDYVANDFETGAEQADVILLAVPVRTTLAYLAVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMAQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPIHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            23456..24751
                     /gene="aroA"
                     /locus_tag="KMT45_000382"
     CDS             23456..24751
                     /gene="aroA"
                     /locus_tag="KMT45_000382"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBE7003870.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEMIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFMTTLFGDASLSKRPMGRVMEPLREMGADLQGKENEQYLPITVTGTNSLSPIRY
                     NMPVASAQVKSALLFAALKAEGTSVIVEKERSRNHTEEMIRQFGGRISVEGKTITVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            24744..25253
                     /locus_tag="KMT45_000383"
     CDS             24744..25253
                     /locus_tag="KMT45_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBE7003871.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQRLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            25250..26086
                     /gene="pheA"
                     /locus_tag="KMT45_000384"
     CDS             25250..26086
                     /gene="pheA"
                     /locus_tag="KMT45_000384"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBE7003872.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPACLRALFRKQV
                     DLAVVPVENSLEGSVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIIGDRKMTSQQPAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(26149..27885)
                     /locus_tag="KMT45_000385"
     CDS             complement(26149..27885)
                     /locus_tag="KMT45_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBE7003873.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(27882..29390)
                     /locus_tag="KMT45_000386"
     CDS             complement(27882..29390)
                     /locus_tag="KMT45_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBE7003874.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFLLYTEKRPFSSKQGSKQKQSITHSLMDSDDSFTIYPVSTFSEESGYVINT
                     DSKDTLRKFVQKFRQEVFPGDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLA
                     YQHELLPLLTNHEQQASSYLTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAIN
                     RFYALLNALADEHVGNEELSLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAK
                     KDNFDILTLIYEFVQENYEKEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVR
                     ISKAKLFLTETRLTLTEICEKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            29663..30949
                     /locus_tag="KMT45_000387"
     CDS             29663..30949
                     /locus_tag="KMT45_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE7003875.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEYSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            31086..31955
                     /locus_tag="KMT45_000388"
     CDS             31086..31955
                     /locus_tag="KMT45_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7003876.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            31952..32839
                     /locus_tag="KMT45_000389"
     CDS             31952..32839
                     /locus_tag="KMT45_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7003877.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMSGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            33006..33689
                     /locus_tag="KMT45_000390"
     CDS             33006..33689
                     /locus_tag="KMT45_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBE7003878.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            33686..34459
                     /locus_tag="KMT45_000391"
     CDS             33686..34459
                     /locus_tag="KMT45_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBE7003879.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMVKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            34456..34854
                     /locus_tag="KMT45_000392"
     CDS             34456..34854
                     /locus_tag="KMT45_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBE7003880.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            35039..35710
                     /locus_tag="KMT45_000393"
     CDS             35039..35710
                     /locus_tag="KMT45_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE7003881.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            35769..36209
                     /locus_tag="KMT45_000394"
     CDS             35769..36209
                     /locus_tag="KMT45_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003882.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            36221..36889
                     /locus_tag="KMT45_000395"
     CDS             36221..36889
                     /locus_tag="KMT45_000395"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBE7003883.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            36953..37576
                     /locus_tag="KMT45_000396"
     CDS             36953..37576
                     /locus_tag="KMT45_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBE7003884.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            37632..38018
                     /locus_tag="KMT45_000397"
     CDS             37632..38018
                     /locus_tag="KMT45_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBE7003885.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            38231..39622
                     /locus_tag="KMT45_000398"
     CDS             38231..39622
                     /locus_tag="KMT45_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBE7003886.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            39969..40616
                     /locus_tag="KMT45_000399"
     CDS             39969..40616
                     /locus_tag="KMT45_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBE7003887.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    40745..40961
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            41009..43654
                     /locus_tag="KMT45_000400"
     CDS             41009..43654
                     /locus_tag="KMT45_000400"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBE7003888.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            43964..45277
                     /locus_tag="KMT45_000401"
     CDS             43964..45277
                     /locus_tag="KMT45_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBE7003889.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            45267..45920
                     /locus_tag="KMT45_000402"
     CDS             45267..45920
                     /locus_tag="KMT45_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBE7003890.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            45975..46667
                     /gene="radC"
                     /locus_tag="KMT45_000403"
     CDS             45975..46667
                     /gene="radC"
                     /locus_tag="KMT45_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBE7003891.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            46817..47017
                     /locus_tag="KMT45_000404"
     CDS             46817..47017
                     /locus_tag="KMT45_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBE7003892.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(47271..48950)
                     /locus_tag="KMT45_000405"
     CDS             complement(47271..48950)
                     /locus_tag="KMT45_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBE7003893.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(48952..49164)
                     /locus_tag="KMT45_000406"
     CDS             complement(48952..49164)
                     /locus_tag="KMT45_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBE7003894.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            49557..51287
                     /gene="efrA"
                     /locus_tag="KMT45_000407"
     CDS             49557..51287
                     /gene="efrA"
                     /locus_tag="KMT45_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBE7003895.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            51284..53044
                     /gene="efrB"
                     /locus_tag="KMT45_000408"
     CDS             51284..53044
                     /gene="efrB"
                     /locus_tag="KMT45_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBE7003896.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            53139..>53309
                     /locus_tag="KMT45_000409"
     CDS             53139..>53309
                     /locus_tag="KMT45_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003897.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKV"
BASE COUNT        17282 a         9425 c        11572 g        15030 t
ORIGIN      
        1 ccataaaaag aaacaaatga ttttgaacta gctagtttaa tgaaagccaa gaaaatgcta
       61 taattaacaa gattgataaa agaaaggatg ttgttaatgt catccaaagc tgaattatta
      121 aacgggatga acccccgtca gaaagaagct gttcttcaca ctgacggacc attactgcta
      181 atggcaggtg caggaagtgg aaagacgaga gtgctgacac accgcattgc ctatttgatt
      241 gaggaaaaag aagtcaatcc ttggaacatc ctagcgatta cttttaccaa taaggctgca
      301 aaagaaatga aagaacgtgt caatgcgatt ctcgcatctg gaggcgaaga tgtctgggta
      361 tcgacttttc actcgatgtg tgtgcgtatt ttaagaagag atgttgattt tatcgggtat
      421 aaccggaact ttacaatcat cgattcttct gaacagctga ccttgatgaa acggatacta
      481 aaagaactga acattgatcc gaaaaaatat gatcctcgaa gtatattagg aacaatctct
      541 caagcaaaaa acagcctgca gacccctcag gattttgcaa aaatgcaagg cagttattac
      601 gaagagattg cggcaaaatg ttatgctgct taccaaaaag agcttcaata taatcaatgt
      661 atggactttg acgacctgat catgaatacg attcgattat ttgaagaaca tcctgattca
      721 ctcacatatt atcaaaacaa attccattat attcatgtag atgagtacca agatacaaat
      781 cacgcacagt atacgctagt caatttattg gcaggtagat ttaggaacct gtgcgtcgtg
      841 ggagatgcag atcaaagtat ttacggctgg cgcggtgcag atatgcagaa catcttggat
      901 tttgaaaaag attatccaga tgctgcagta attttgctag aacaaaacta tcgctccacg
      961 aaaaacatcc ttagcgcagc aaaccaagtc attgaaaaca atagcaatcg aaaaccaaaa
     1021 aacttgtgga cagaaaacaa agaaggaaac aagatcactt actaccgtgc agataatgaa
     1081 cgagacgaaa cccgttttat cgtagaccgg atgcaagaag agattcgaag caatcatcgg
     1141 aattacggcg actttgccat cttgtaccgg acgaacgcac aatctcgtgt aatggaagaa
     1201 actctgttga aagcaaatat tccttataaa atggttggag ggcacaagtt ctacgatcgt
     1261 aaagaaatca aagatatttt agcgtatttg aatgtgctgg caaatcctca agattcaatc
     1321 agttttgagc gtatcgttaa ttcgcccaaa cgaggcatcg gaccaggatc tatcgaaaaa
     1381 ctacgcagtt ttgcttctct tcatgaatgg ccactgcttg aagcagcgca aaatgtggat
     1441 ctggcaaata tcggtggaaa agcaggacag caattaggtg catttggaga gatgatccaa
     1501 gaagtcactc agatgatcca atacttgaca gtgacagaat tgacgaaaga ggttttggat
     1561 cgtagcggat atctagaaga cttaaaaatc caaaatacat tagaagcaca agcacgcatc
     1621 gaaaacttgg aggaattttt aacggtcaca caagaattcg acaagcaatt cgaacagcaa
     1681 aatgaagaag acgctgatgc accagaagaa aaactaacgg tcttcttgaa tgatctggca
     1741 cttgtttccg acattgataa tttagaagaa gatgcttccc aagtgacatt gatgactttg
     1801 catgcagcca aaggattaga attcccagtt gtattcctga ttggtctaga agagggggtt
     1861 tttcctttat ctcgagcatt gatggaagaa agcgaactgg aagaagaacg ccgtctcgca
     1921 tatgtgggga tcacgcgagc agaagaagcg ttgtatttaa caaatgcttt ctccagaaca
     1981 ttatatggac ggacgcaata taaccgacct tctcgttttg tagaagaaat cgatcaggag
     2041 ttactggaaa tcgaaggcat gcgtcctacg cctaaaaaaa cacctgtatt cgctaaaaaa
     2101 acagcatata gctataaaca gccagagaca gcggttgttc cctctaaaag tgctactgga
     2161 ggggaaaata acaactggaa acctggagat aaagtcaaac acaaaaaatg gggtctagga
     2221 acagtcgtcc gagtaagcgg gacgtcgaag gatctagaac tagatgtggc tttcccaagt
     2281 caaggagtaa aacgtctttt ggctgccttt gcgccaattg aaaaagctta gtagtggagg
     2341 gtaaatgatg gaacatgaaa tgacgattga cgaagctgct aaacgagcag aagaacttcg
     2401 tacaagactg aaccaatggt ctcgcgaata ctacgtagaa gataaaccaa cagtggaaga
     2461 ctatgtttat gataaagaat acgcagaatt agtagcaatc gaggaacaat atcctgattt
     2521 gatcacgtct gattctccca ctcaacgagt aggcggaaaa gtattagaag gttttgaaaa
     2581 agtcactcat gatattccgc tttacagttt gaacgacgtt ttcagcaaag aagagctgat
     2641 agcttttgat caacgtgtac aaaaagcagt gggacgtgta gttgactatt gctgtgaact
     2701 aaaaatagat ggactttccg tttctttgcg ttatgaagat ggaaatttcg tgagaggtgc
     2761 gactcgcggg gatggaactg taggagaaaa catcactgaa aacctaaaaa ctgtccggtc
     2821 ggtcccaatc aaattaaaag aaccaatgaa catcgaagta agaggcgaat gtttcatgcc
     2881 aaaacggtca tttgtccaat tgaaccaaga cagagaagca gagggaaaag atatctttgc
     2941 gaatcctcgg aacgccgcag ctggttcttt gcgtcagtta gattctaaaa tcacagcgaa
     3001 aagaaatctg gacacttttt tatacacagt agctgatttc ggtcctatgc aagcaaaaac
     3061 ccagtatgat gcgctagaag agctagaaaa aattggattc cacacgaatc gggaaaaacg
     3121 tctatgtcat tcgattgatg aagtgtggtc atacattgaa gaatatcatg acaaacgtgt
     3181 ggatttgccc tatgaaatcg atggaatcgt catcaaagtg aatgaattct cgctacaaga
     3241 tcaacttggt tttactgtca aagcaccgag atgggcagca gcttacaagt ttccgccaga
     3301 agaagttgaa acgctaatcg aaaacatcga atggacagtc ggtcgaacag gggtagtaac
     3361 gccaacagct atcatgacac cagtccgagt agcaggaacg acagtaagcc gtgccagcct
     3421 gcataacggt gattatatta agttgaagga tatccgcttg aaagatacag ttctgattta
     3481 taaagcggga gacatcatcc cagaagtttc gcaagtcgtg ttagataaac gacctaaaga
     3541 cagcgaagaa taccaattgc ctacacattg tccagtttgt ggaagtgaac ttgtccattt
     3601 ggatgaagaa gtagccttac ggtgcatcaa tcctaaatgc cctgctcaaa tgaaagaagg
     3661 tttgaaccac tttgtttcaa gaaatgcgat gaatatcgat gggttggggc cgcgcgtact
     3721 agaacagatg tatgataaaa aactagtagc agatgttgct gatctttaca aattgacaga
     3781 agaagagtta ttgacgttgg acaaaataaa agaaaaatca gcaaataata ttttgacagc
     3841 aatcgacaac agcaaagata attcggtcga gcggttgatt ttcgggttag gaatccgaca
     3901 tgtcggagcg aaagcagcaa aaattttagc agaacatttc ggtgatctag aaacattgag
     3961 taaaagtgac tatgagtcga ttattgcgct tgatacgatc ggtgatatca ttgccgacag
     4021 tgtagtcact tatttcagta acgaagaagt ccatgaattg atgaatgaat taaaacaagc
     4081 aggcgtcaat ttcgaataca aagggcttcg aaatgcccaa ttgcaagaag tcgagtctcc
     4141 ttttaaagaa aaaacagtag tccttactgg taaactaaca cgatttactc gtgaagaagc
     4201 aaaagagacg atcgaaaatc ttggcgggaa agtgactggt agcgtatcga aaaaaacgga
     4261 tatcgtggta gcaggagaag atgctggcag caagctgacc aaagcacaag aattaggaat
     4321 cgaagtttgg acagaagatc aaatggcaga tgcattggct aaaagtcgct cagtagaaga
     4381 atgaaaaatg agttttttca gacaaatttc aataaacaat ttcagacacg gcactcttgt
     4441 gttaaaatag agagcaaagt ttttaactcg tttagaaaga aggggtagta tggcgattag
     4501 tgaagaacaa gtgaaacacg ttgccaaatt agcaaaactt gcgttcgcag atgaagaact
     4561 tgcaagtttt accgaccaat taggtaaaat catcgatatg gttgaaatgc tggaagaagt
     4621 agatacagaa ggtgttccat ttacgtcgaa tgtggttgaa acagtaaacg tgatgcgaca
     4681 agatcgggcg caagctggat ggaaccgcga tgaattattg aaaaatgtgc cggaaacaga
     4741 agacggcttt atcaaagtac ccgcaatcat cgataatggg gaggctggcg cataatgaca
     4801 gaattacaca atcgttctct agaagaactg catcatatgc ttgtatcgaa agaaatcact
     4861 gtacaagatt tgacaaaagc aacatttgaa cgaatcaaag aaacagagcc tgaggtcgaa
     4921 gcattcatta cattgaacga agaaaaagca ttggaacagg caaaagcttt agatgaaaaa
     4981 gggattgatg aaagcaacgt ccttgctggt attccaattg gtatcaagga taatatcgta
     5041 acaaaagata tcttgacaac cgctgcatct aagatccttt acaattttga accaatttat
     5101 gatgcaacgg tcatggataa aatctatcaa gcagatatga tccctgttgg taagctaaac
     5161 atggatgaat ttgctatggg tggaagtaca gaaacttctt atttcaagaa aacaaaaaat
     5221 gcttgggacc caacaaaagt acctggtggc tcttctggtg gttctgcagc agcagttgca
     5281 gctggacaaa ttccagtttc tttaggtagt gatactgggg gaagtatccg ccaaccagca
     5341 gcttttaacg gcatcgtagg gatgaaacct acttatggcc gtgtctcacg ttttggtttg
     5401 attgcctttg cttcatctct tgaccaaatc ggaccaatga cccgtacggt gaaagataat
     5461 gcattagcat tgacagctat cagcggttat gacgaaaaag acggtacttc ttcaggcgtt
     5521 tcagttcctg attttgcaga gggactgaca ggcgacatca aaggaatgaa gattgcttta
     5581 cctaaagaat acttaggcga aggtgtagcc cctggcgtaa aagaagctgt attgaaagca
     5641 gcagaaacat tccgttcatt aggtgctgtt gtggaagaag tcagcttgcc tcattcaaaa
     5701 tacggtgtgg ctgtttatta tatcattgcc tcatcagaag caagttccaa cttgcaacga
     5761 ttcgatggta tccgctacgg ttatcgctct gaaaatgtga aatcattaga tgatgtatat
     5821 gtcaactcac gttcagaagg attcggtgaa gaagtaaaac gccgtatcat gttaggtaca
     5881 ttctccttaa gtgcgggtta ttatgacgct tacttcaaaa aagcaggaca agtccgtaca
     5941 ttgatcaaac gagattttga aaaagtcttt gcagattatg acttgattat cgggccatct
     6001 tcaccaacag tggcattcgg aatcggtgaa aacattaacg atccaatcac aatgtacatg
     6061 agtgacatct taacgatccc tgtcaacttg gctggacttc caggtatgtc tgtaccggca
     6121 ggattgtcag aaggattacc agttggattg cagatcatcg gtaattattt tgatgaaaaa
     6181 acgatgtatc aagcggcata tgcgtttgaa caagcaacag aattccacac acaacaacca
     6241 gctattttag gagggaaaga cgaatgaatt ttgaaacagt cattggacta gaagtccacg
     6301 tcgagttaaa aaccaactca aaaattttct ctccagcgcc agctcatttt ggtgcagaac
     6361 caaacagcaa tacaaacgtg atcgactggg gttatccagg cgtgctgcca gtaatgaata
     6421 aagctgcgct ggaattcggg atgaaagcag ctttggcatt aaactgtgaa atctctaaag
     6481 atactcattt tgaccggaaa aattactttt acccagataa tccaaaagca tatcaaattt
     6541 cccaatttga tcaaccaatc ggtcatgacg gatggatcga aatcgaagta gaaggtaaaa
     6601 agaaaaaaat ccggatcgaa cgtgtccatt tagaagaaga tgctggaaaa aacatgcatg
     6661 gtattggcgg ttattcttac gttgatttga accgacaagg cacgcctttg atcgaaatcg
     6721 tttctgaagc tgatatgcgt tcaccagaag aagcatacgc ttatctagaa gcattgcgat
     6781 caattatcca atttacagaa gtcagcgatg tgaaaatgga agaaggctct atgcgctgtg
     6841 atgccaatat ttctcttcgc ccttatggac aagaagagtt cggtacgaaa gcagaattga
     6901 aaaacttgaa ctctttatct ttcgtgaaaa aaggtttagc ttttgaagaa aaacgtcaag
     6961 caaaagttct tctttcagga ggagaaatcc aacaagaaac acgccgtttt gacgaaacaa
     7021 cgaataaaac agttttgatg cgtgtcaaag aagggtctag tgattatcgt tacttcccag
     7081 agcctgatat cccgaaattc gtgatcgatg acgaatggat tcaaaaagta aaagctagtt
     7141 tgcctgagat gccagcttct cgtcgcgaac gctatatccg tgagtttggt cttccagagt
     7201 atgacgcaat ggtattgaca cttacaaaag aaatgtctga tttctttgaa gctgttttgg
     7261 aaaatggtgc agatgccaag caagcatcta actggttgat gggtgaagtt tctgcttact
     7321 tgaacagtga gaaactggaa ttagctgaga caaaattgac acctgagaac ttagcaggaa
     7381 tgattcgttt gatcgccgat ggaacgatca gctcaaaaat cgccaaaaaa gtcttccgtg
     7441 aattgatcac aaatggcgga gaagcccaag aagtagttga aaaaaatggt tgggtgcaat
     7501 tatctgatcc gaacaaacta ctgccaatca ttaatgaaat tttggataac aaccaacaat
     7561 ctgtcgatga tttcaaaaat ggaaaagatc gtgcggttgg cttcctagtc ggtcaaatca
     7621 tgaaagcaac taaaggacaa gcaaatcctg gtgtcgtgaa taagctactt aaagatgaat
     7681 tagcgaaacg atagaggctg aaaatgaaaa aagcacgcgt aatttataac cccacctcgg
     7741 gaaaagaatt attgaaaaaa aatttggcgg atatcctaca ggcattagaa gaagcaggat
     7801 ttgaagccag cgcctatgca actacaccag aacctgattc agccaagaat gaagcacgta
     7861 gagtcgcaga actaggtttt gatctagtag tggcagctgg tggcgatggc acaatcaacg
     7921 aagtggttaa tggaatcgct ccgcttgaac accgtcctaa aatggccatc atcccagctg
     7981 gtacaacgaa cgactatgct cgagcattaa aaattcctag agataatatt aaagcagcag
     8041 cagaagtgat caaaaagcat caaaccgtaa aaatggacat cggcctttct gataacagtt
     8101 acttcatcaa tatcgctgcc ggtggctatt tgacagaatt gacttatgaa gttccatcag
     8161 aactaaagag tatttttggt tatcttgctt atcttgcaaa aggagcagag atgctccctc
     8221 gagtaaagcc catcaaaatg cgcttgaagt atgatgaagg ggaatatatc ggaaatgctt
     8281 cgatgttttt cctagggctg acgaactctg ttggaggatt cgaacagatc gcgccagatg
     8341 ccaaattgga tgatgggaag ttttctttaa tcattgtgaa gacagcaaat atctttgaga
     8401 tcttgcatat tgcggcactc atgctaaatg gcggtcgtca tgtcgatgat ccaagagtaa
     8461 tctataccaa aacaagcagt ttgttcgtcg aaacagaaga tgatatcaat cgaccagtaa
     8521 tgatcaactt agatggtgaa tatggtgggg aagcaccgat gcatttccaa aatcttcatc
     8581 agcatattga gttctttgcc aataccgatc agattcctga tgaagcaatc acaggcacag
     8641 atgaagaaga attagaggtt gtcagcaaag aattcgtaaa agaagtcgaa cgtttaacgg
     8701 atgaagatat cgacggtgac ggaaaaatag cagacgaaac acctaatcaa aagaattaat
     8761 caaaagccgg tgctgaacgt agtaaggtca gtgccggttt tagttgtgta attagctgat
     8821 ttatagtaga gttcagtagg attgaaaaat aggcttaaag gagcaaaata tgacagaaca
     8881 gcaaatagta aagaaaaacg aacgatatac agtcgaaatc gtcgacttga gttatgaagg
     8941 aatgggggta gcaaaaatcg atggttatcc attatttatc gaaaatgcac tccctggaga
     9001 agaagtagaa atactagtag tgaaagcagg caataagttt ggatatggaa aagtcgaaac
     9061 atttattaca caatcacctg atcgtcagcc tgtagaaaac aatgcattac ttcgcacagg
     9121 tatcgcacct ttagcccatt tgaagtatga acaacagctt gtcttcaaac aaaaacaagt
     9181 agagcatgta atggcgaaga ttgctaagat gccagaagtt cctgtacttc caacggtcgg
     9241 aatggcagaa ccatttggct accgtaataa agcacagatc cctgttcgcc gtatcgatgg
     9301 acagttagcc accggttttt acaaaaagaa tagccatgat ctagtcccta tcgaagattt
     9361 ttatatccaa gatcctgtca tcgatcaagc aatcaaagta gtcagagata tcttgcaacg
     9421 gttccaagta cgaggatata atgaagaaaa aaatgaagga cagcttcgtc acatcattat
     9481 ccgtcgtggt cattattccc atgaaatgat ggtcgtttta gttactcgta aagaaaaatt
     9541 ctttaaatcc aaggaaattg ctgaagccat tcatgaagaa ttgccagaag tggtttcggt
     9601 catccaaaat atcaatgaag aaaaaacaaa tgtgatttta ggagacaaag aaaaagtttt
     9661 atacggcaga gactatatcg aagatcagct attaggaaaa acctatcgaa tctcagcgaa
     9721 gtcattttat caagtaaata ctcaacaagc agagtatttg taccaaacag ccatcgaatt
     9781 tgccgagttg gataaaaaag acattgtgat cgatgcttat tcaggaatag gtacaatcgg
     9841 attgtctcta gctgacagag tagataaggt atatgggatg gaagttattc cagaagcaat
     9901 tgaagatgca cagttcaatt ccttaaataa taaaatcgaa aatgttcatt atgaagtcgg
     9961 aaaagcagaa aaagtcatga aaaaatggca agaaaaaggg gtcaaaccat ctgtgatcgt
    10021 agtagaccct ccacgtaaag gattagatca tcattttatt gaatcagcaa tcgaaatgtc
    10081 accagaacga atcgtctata tctcttgtaa tccagcaact tttgcaagag atgctaagtt
    10141 attggcagaa gcaggttatg ttgtgaagaa agttcagcct gtagatcttt tcccgcaaac
    10201 ccatcatatc gaattagtcg cttcattcgt taaaggagaa taaaaatgga ccaaaaagaa
    10261 aaaatgaaag aattgatcaa aaagaaacaa ggtggaggcc gatccaaaca aatggaaaca
    10321 cctaagaatg atcgtaaaaa catgagaaaa ggccctaaaa tctttaataa atagacgtgc
    10381 taaagaaaga ctaagaagcg ttaacttttg ttattgtttc ttagtctttt tagttagtct
    10441 acattatttt ggcgatttgc caataaggac tgatgagttg tgcaaaatca aatctgggtc
    10501 agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa ctgattgatc
    10561 aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca tgtttgacgc
    10621 ggaaattatt attcgatttg ggcggcatca ggggggatga cgacgtagca gacccattat
    10681 tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg tgctgggagt
    10741 tttgttcatt tagagaaagt ctctgaagat aaattagttg tcttagattt tgactgcaat
    10801 gaaaaatggc gaactggaag agcaccagca agtgattgac cggatctacg aagcgacacg
    10861 atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt caacagaaga
    10921 agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag cattgcagaa
    10981 gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata acactatggt
    11041 ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa aattcttaaa
    11101 tttatcggat ggttcttcgg aatcattctt gggatcacgt ttgcaatctt tcttgccttc
    11161 cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa tactgttcaa
    11221 atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa aacagatttg
    11281 acgtatgagt ccaagtataa agagaatacc tatgacatct actatccaaa aaacagcaag
    11341 gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg agataaatcg
    11401 ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt agttgcgatg
    11461 aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt gaatgaactg
    11521 atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga acaactattt
    11581 tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat acaaacgaat
    11641 gacgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag tctgaaagga
    11701 accatttctt attgcggacc tgttgatttg aagctaacag cacagcagca ttcggatgat
    11761 cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac aaaagattgg
    11821 aaaacgagtg agcaattgaa agaagccagt gtggctgacc atgtttctaa ggaatttccg
    11881 ccaacgtata taactgacgg aaatgcttac tcattccaag aacaaggtca agcattagaa
    11941 aaccgattga cagaattaaa agtacccgtg caatccttgt tttatacaga tacaaaaaaa
    12001 gagattactc atgaatatca atttgattac acgttaaaag aatcgcaaaa ttgttaccag
    12061 cagacattgg attttgtgaa taagtataag taagaggctg tgatcctgcc gtttagtttt
    12121 gagtatgaag aaactaagcg gctttttcac aacctctttt tcaatatctt tttgttcttg
    12181 ggataaaact atcccgaaca atcaatcgcg caggataagt caaaatgatc ggttcatcac
    12241 gtataccgtc aattcgctcc aacgccattc gaacagctgc ttgcccaatt gattttgcat
    12301 tgatttgaac actggtcaag ctaggggaga ggtttgcaat tgtctcgata ttatcgaagc
    12361 ttgctacaac aatctcttct ccaatttgga tgtttttttg cttcaatgcc cgatagacac
    12421 cgattgccag tggatcgctc ccgacaagaa tggcagtagg aagaggggat tcattttcta
    12481 atagatcagt cgcaagtacc tcaccttgtt tttctgtcca gccacctaga cgataagaca
    12541 tatatttttc taagtgatga tcgatcataa aatgctgata ggcacgcatc ctcttttcgt
    12601 ttatagaaat ctgcttccca ccgaattcat ccatatcgac tttatatccc ccgatatagg
    12661 ctattctttt atgaccattt tctaagaaca attccagtac ggttttagtc atccgttcaa
    12721 gatctgcata caccatatcg acctcttgct ggatgtcagg gttatccacg acgactattt
    12781 gcatgttttg ttttaacaaa gcggtaatcg attctttcgt aacagtccca acaacgatca
    12841 ccgcacctaa ctgatctaga tcttcccatt gtttaggatt atcagataga ttatagattc
    12901 gattcatccc taaatgcaga cgtcgagctt cttcttccat ccctactcgc aaatgtttgt
    12961 aatagacatc ctgttcttcg ctcgctttgc cgatcgtaga gatcacacca atcgaaggca
    13021 ttctcttttt gatatattta cgagtactga tgtcataatt caacgcagca gctgcctcta
    13081 ctatccgttg ccgagtagcc tgagccacat ggatcgtctg atcgttattc aacacgcggg
    13141 aaactgtagc aggagagaca tttgcctttc gtgcgacatc tctaattcca accattttta
    13201 cacactcctc aaaaaaataa aaaatttcta ttgacaaaat tgtttagtaa acagtataca
    13261 ggaattataa aaaaagtaaa cggtttccgt tttgatggga gtgaaatgat ggatattacg
    13321 catagaaaaa cagataaaaa tttcatctta tttgatgaaa atagccgagt cttccattta
    13381 cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt ggcgcatatt
    13441 tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg aagagatcgt
    13501 ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga tacactaccg
    13561 caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat cattcagcgc
    13621 aagaatgggt cagacttact ggatctgaga tatgacagcc attatattac agatgggaag
    13681 ccatagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc acaaacgttg
    13741 atgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta taccatattt
    13801 actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg agaaacaatc
    13861 aaactagaaa aagcagctag tttccagcta gactttgccc atactaggcg atttgatgaa
    13921 gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca atctgtttta
    13981 tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat gaataacttt
    14041 attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg acttcaattt
    14101 gtttattccg gcaaccattc tttcgaactt gaaaaagatc aaatcaatca attgaaagtc
    14161 gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca gtcttttcaa
    14221 acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag tcagatccac
    14281 catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga acggccgatt
    14341 ctggtcaata actgggaaga cacttacttt gacttcaata gtgaaaaaat caaggcgatc
    14401 attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg ctggtttgga
    14461 aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg caagttgaca
    14521 aatggattac gtgaaatagc agattatgct catagcaaag gggtgaaatt tggtttatgg
    14581 tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacattc tgattttctt
    14641 atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact ggattttaca
    14701 aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga tacgattcct
    14761 cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc gattaccctt
    14821 gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta tgaactactt
    14881 gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg tggaggtgga
    14941 cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga taacacggat
    15001 gcagtggagc gcatgaaaat ccaatatgga acaagtcttg cttatccgat ttcgtccatc
    15061 acagctcatg tatcagctgt gccaaatcat cagaccgaca gatctacttc gttgaaaaca
    15121 agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac aaagttgtct
    15181 cctgaagaaa aaaagcaagt caaagaccaa attatatcct atcaaacgat tcgtccggtg
    15241 atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat tgccgcatgg
    15301 atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat actagcttca
    15361 gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc actttatcag
    15421 gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg actttacttc
    15481 cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt atgagagaga
    15541 aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt tttagagtga
    15601 accaatcaaa tgcacatagc gatcatgttt gctatgcaac aagggaagaa gtagaaacca
    15661 catcctccct taaacagtct ttaaatggaa catggcgttt ttgttattcg ccaaatcctt
    15721 cggtacgtcc aaaaacattt tataaaaaag attttgatgc ctgttctttt gatgaaatca
    15781 ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc aatacgatgt
    15841 atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct gagaaagagg
    15901 cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg acgacttttc
    15961 gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt gtagaacaag
    16021 caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg ttcactccgt
    16081 ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg gaggtttaca
    16141 aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt gggatcttca
    16201 gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg atcaaacctc
    16261 gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt gagcaagtag
    16321 ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa atctatcaac
    16381 agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa aatgtggcac
    16441 tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata gacgaagaaa
    16501 aaagagtaac agaatatatt gactattcct tcggttttcg taaaatcgaa atcaaagata
    16561 aaatcatata cttcaatgga aaacggatga ttatcaacgg ggtaaatcga catgagtggc
    16621 atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg gctatcataa
    16681 aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc acttggtatt
    16741 atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa tcccatggct
    16801 catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca gtacctcagt
    16861 ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa aaccatccaa
    16921 gtattctttt ttggtctctt ggaaatgaat cctatgcgga agtaaacatt cgacaaatga
    16981 acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg aattttccaa
    17041 atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg ccaccagaag
    17101 agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcctatgc gagtatatgc
    17161 atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt gatcgttatg
    17221 aaatgtacca aggaggctat atatgggatt ttaaagatca ggcaatccaa gtgactgatc
    17281 caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa ccaagtgact
    17341 atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc gccatgcagg
    17401 aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac ggaaaaagtt
    17461 gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca ttatattttt
    17521 tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg gctataccgg
    17581 gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag cggatttgga
    17641 tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga aatagaagta
    17701 ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca atattcaagt
    17761 aatgaatacg ctgaaacggt tgaaattatt tttacactgg aaatcaacac agtaccaaaa
    17821 acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt caatatgcat
    17881 tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt cattatgcca
    17941 acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc ggatcggatg
    18001 gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc ataccttgtt
    18061 ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag aaatcaaacg
    18121 ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca gcaggatacg
    18181 ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc aacacatata
    18241 gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt aagaggagtc
    18301 gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag cagcgaagag
    18361 gaccatgaaa tcggctttat tattcagtag caatctttag attattattt gaaaaacgga
    18421 gtttacaaga caaagctgct ttattctata tgtcatgaac aagagagtaa aaaagttctc
    18481 agatactttt gttcatgact attttcttca ataaaaccaa ttgttatttt tctgaaaaac
    18541 aaagttaatt atcagaaaat taaaaaagtt tcataaaaat gattgacagt cgattttttt
    18601 atgtctatac taggtacaat tcttacatca gtttggagcg attttttatg aaatctagaa
    18661 aacttacaaa actgaatacg tattacttta gctattttag ataggtttgt attcatgact
    18721 tcgtgggtac aaacaaacca ggtttgtatc tatgatggct aaagattttt aaacacgact
    18781 tacagccacg tagagaaatt catctatagt ggctatttta tatttttgca gcgcttttta
    18841 aggctcgttg aagatgttca ctgtagaggc aatttcctac agggacatct ttttttgttg
    18901 attatctggg tcataagtag gaaagaagga gcgaaaatga gaacaacagg acacggtcat
    18961 ttagtaggaa ttaatgtaaa accaccttga aaaagtagag aaaatcagcc aagatagata
    19021 agtaaacaaa agaaagcggg aaaaacaatg atagtaatta tgaaaaaaga agcaacggaa
    19081 gaacaaataa aaaaagtcgt cgatcatatc gaaaaagaag gattcaaagt ctcaatcgat
    19141 caaggaacag aacgagtagt gattggacta aaaggtgata cgcgaagttt acaagaagga
    19201 gcatttacac gctatgaagg agtagaaaat gctgtgcgta ttttaaacac atacaaattg
    19261 acttctcgag aattccatcc tggcaataca gtcgtggatg tagacggagt aaaaattggt
    19321 gacggcagtt tcgtgacgat ggctggtcct tgttctatcg aaggattaga ccaaatcaga
    19381 gaatgtgcgc gaatggccaa agccgggggc gcaaaaattc tgcgaggtgg tgccttcaaa
    19441 ccacgtactt ctccgtatgc tttccaaggt ttagaagaag aaggattgaa atacatccgt
    19501 caagcggctg atgaatttgg aatgaaagtg attactgaag tgatggatga agggcatatc
    19561 gacatggtgg ctgagtacag cgatatccta cagattggcg cacgtaatat gcaaaatttt
    19621 aaactgctat ctgctgtcgg taaaacaggt aaacctgttg gattgaaacg tggaatttcc
    19681 ggtacgatca atgaatggct taatgctgca gaatacattg cagtcgaaga caaatctcct
    19741 gtcattttta tcgaacgtgg gattcggact tacgaaacag cgacacgtaa tacgtttgat
    19801 ttaagtgctg tcccattgat gaaaaaatta actcatttcc ctgtgattgt tgatccaagt
    19861 catggtacag gaatttggga acttgtacca ccaatggctc gtgcgggtgt ggcaagcgga
    19921 gcagatggca tgatcgtaga gatccatcct gatcctgcaa atgcttggtc agacggtccc
    19981 caatcgttga atgaaaagac ttactcacgc atgatgaaag aagttgcgat catgaaagaa
    20041 gcaatggaaa agattcagac actcgattaa ttccagcttt ttaaaagaaa gaagtgaaat
    20101 gaatgcttac tgtacatgtt gataaccaaa aatacgatat tcatattgaa aaaggcgcac
    20161 tagaacaggc tggatcatgg cttcaaacga tttggagacc tcaaaaagta gctgtgatca
    20221 ctgatacaaa cgttgctcca ctatatgcag aaaaaatcat gttccaattg agacaagcta
    20281 attttgaacc agtcctttca atcgtaccgg cgggtgagac cagtaaatct ttagaacaag
    20341 cagctgagtt atacgatttt ttagcagata atgattttac ccgcagcgat gggatacttg
    20401 cacttggtgg aggaatggtg ggtgatctag ccgcattcgt ggcctcgacg ttcatgcgtg
    20461 ggatccattt cgttcaaatt ccgactacgc tacttgcaca agtagattct agtattggcg
    20521 ggaagacagc agtcaataca aacaaagcga aaaaccttgt cggaacattt agccagccag
    20581 ctggtgtatt gattgatcct gaggttctaa aaactttgcc ggtacgtcgt ttacgagaag
    20641 gaatcgctga aatcatcaaa tcagcagcta tagcagatgc aacgctttgg aaacagctga
    20701 acgagctgaa agacgaaaaa aatcttctgg aacatgctgt tccaatcatt actgctacgt
    20761 tggaaatcaa acgaaaagta gtggaagaag atgtctttga ccaaggaaat cgacttattt
    20821 taaattttgg tcatacaatt ggccatgcaa ttgaaaatac agcaggatat ggggtagtca
    20881 gtcatggaga aggagtagct attggaatgg tagccatcac ttctcatgca gaaaaaatcg
    20941 gcttgtctcc aactggatcg acagctgctt tagaaaggat acttcaaaaa ttccatctgc
    21001 cgattacgta tgatctaagt catccagatg aattattcca cgcgataacc catgataaga
    21061 aagcaagagg aagccaattg aatatcatct tgctagaaaa gattgggcaa gcaaaaatcg
    21121 ttcctgttcc cacagagaca ttcaaagatt acttaggagg actgtaagat gcgttatatg
    21181 actgcgggag aatcccatgg accagaatta acagcgatta ttgaaggact accagcagga
    21241 ttacctctgt cagaagaaga aatcaatcac gaactcgctc gcagacaagc tggttacggc
    21301 agaggaggaa ggatgaagat cgaaaaagat cgtgttcgga tcacgtctgg catccgtcac
    21361 ggcaaaacaa ttggttcacc tgtcacttta gtggtagaaa ataaagattg gaaaaattgg
    21421 acaactgtca tgtctgtagc agaagtgcct gaaaaagacc aatcactgcg gcgagttgca
    21481 cgtccgcgtc caggacacgc ggatttggta ggcggtatga aataccatca tcgagatctt
    21541 cggaatgtgc ttgaacggtc ctctgcaaga gagacgacca tgcgtgtagc aatcggcgca
    21601 attgccaaga aaattctgga agaacttgac atcatcgtag caggacacgt gatttgtctt
    21661 ggtggaatcc aagcagaaat acctggaaat atctcgattc aagaaatcaa agaaatttct
    21721 gaagcgtcag aagtacgagt tgttgatcca gcagttgagc aaagaataaa agatttgatc
    21781 gatcagacaa agaaaaatgg agatacgatc ggaggaattg tagaagtgct cgttggaggt
    21841 gttccagctg gattaggaag ctacgtacaa tgggacacga agctagatgc caaaatcgca
    21901 caagctgtgg ttagtatcaa tgcttttaaa ggcgtagaat ttggggtcgg attcacttct
    21961 ggttcattac caggctcaga agtgatggat gagatcctat gggaagaaga aagaggctat
    22021 ttccgacgta ccaataatct aggcggcttc gaaggcggaa tgaccaatgg cgaaccactt
    22081 gtcattcgcg gggtcatgaa acccatccct acattgtaca agccacttca aagtgttgat
    22141 atcgatacaa aagaacctta caaagcaagt gtagaacgtt cggatagtac agcagttcct
    22201 gcagctagcg tcgttgcgga aaatgttgta gccactgtgg tagcgacaga gctattgtca
    22261 aaattcccaa gtgattcgct tgttgagttg aaagttgcag tgaaggagta ccgtgactac
    22321 ttgaaacatt attaaaaagg gggcaaataa gatggcacaa catgttttta tcgtggggct
    22381 tggactgatt ggtgcttcta tggcactttg tattcgtcag accaatccag aactgatagt
    22441 agttggttgg gatagtcagt caaaaacccg tgagcaagca atagaacagc agatcgttga
    22501 ttacgttgca aatgattttg aaacaggtgc agaacaagca gacgttatct tattagccgt
    22561 tcccgtccgt acaaccttag cttatcttgc tgtccttgaa agaatcaact tgtctgatca
    22621 cgtcatcatt acggatgtca gcagtacgaa gcagcaagtc gtgacatccg ctgagcagaa
    22681 aaatcttcgg tttgtcggtg gacatccaat ggctggctct cataaatcag gtatacaggc
    22741 agcagatgct aaactatttg aaaatgccta ttacatcttt acacctttgg aaaagacgaa
    22801 aaaagatgtt gaaaaactgc aagaactatt ttgcggaacc aaagcaaaat acgtaactct
    22861 tacagcaaga gaacatgacc gtataacagg aatgctaagt cattttccac atattctggc
    22921 agctggctta gtcaatcagg cagaacaatt caatcaagag tatccgcgag ccaaacaact
    22981 ggcagcagga ggctttcgag atatcactag gatcgcttcc tcggatcctg ttatgtggac
    23041 agatattttg ttgagcaata agcagatatt attggaacga ttggcggatt ggcagcaaga
    23101 aatggcacaa atagcagaat ggatcatgac tgagaaccaa tcggaaatct tttctttttt
    23161 caatcgggca aaagaaagtc gagaccagtt gcctatccat aagcaaggcg cgattcctgc
    23221 cttctttgat ttatttatcg atgtgccaga tgaacctggc gttattgcag aagtaactgg
    23281 actgatcgga aaagcgggag tttctttgat taatttgaag atccaagaga ccagggaaga
    23341 tattttagga atactgcaaa tcagttttaa aaaccaacag gatttgttac aagcaaaaag
    23401 aatcattatg tctaatacca gttatcattg ctggatcaaa taggaggctg gataaatgca
    23461 attattacaa caaatacatg gattaagagg aactgttagg ataccagcag acaaatcaat
    23521 ttctcatcgc agcatcatgt ttggggcaat tgcggaagga acgacgacta tacaaaattt
    23581 tttgcgcgca gaagattgtc tgagtacttt acatgccttc cagcaattag gcgttgaaat
    23641 cgaagaagag gaagagatga tcaagattca tggtcgcggt agccactcat ttgtccaacc
    23701 aaccgaatca atcgacatgg gaaactccgg tacgaccata cgtttattga tgggtatttt
    23761 ggctggacag ccttttatga ctactctgtt cggggatgct tcgttgtcta aacgtccaat
    23821 ggggcgagtg atggaacctt tacgcgagat gggcgctgac ttgcaaggaa aagagaatga
    23881 gcagtatcta ccaatcactg tgacaggaac caactcttta tcacctatcc gatacaatat
    23941 gcctgtagcc agtgcgcaag tcaaatctgc tttgctgttt gcggcattaa aagcagaagg
    24001 cacatccgta atcgttgaaa aagaacgttc ccgtaaccat acggaagaaa tgattcgtca
    24061 atttggcgga aggatctcag tagaaggtaa aacaatcacg gtaacaggac cacaaaaatt
    24121 aactggtcaa cagataactg ttccaggcga tatttcatcg gctgctttct ttctagcagc
    24181 agggcttctt gttccggaaa gccaactgtt attaaaaaat gtcggggtca atccaacaag
    24241 aaccggtatc ttagatgtgc tggaggagat gggcgcagcg attacccaga cgaatcacaa
    24301 tgaacagaac caatcggctg atttaagtgt caagacgagt catttgaaga aagcaacgat
    24361 tggtggagaa atcattccac gtctgattga tgaattgccc attctagctt tagtggctac
    24421 tcaagcagaa ggaatcacga tcatcaaaga cgcagaagaa ttaaaagtaa aagaaaccaa
    24481 cagaattgat gcggtggcag aagaattaca gaaaatggga gcaaagataa aagcgacagc
    24541 agacggtctg atcatccatg ggccgacacc tctgcatggc gcaaaagtgt ccagcagagg
    24601 cgatcatcga atcggtatga tgctgcaagt agctgctttg atagctgatg gaccttgcga
    24661 actagaagga gctgaagctg tttcaatctc ttatccggca ttttttgacg atttagcaga
    24721 acttgtgtca ggaggagaag ctcatggcta gcattttgct gattggtttc atgggcgctg
    24781 gaaaaacaac aatcggtaaa gggttagctc aacgtttaca aaaagagtat gtagatttag
    24841 atacaaagat tgaggaacat atccaattgt cgattgccga atattttcac tattacggtg
    24901 aaaaaagctt tcgaaaagtg gagtcagata ttttaagaaa gctgtcaaat gaagataaaa
    24961 ttatcgcgac tggtggaggg attgtccaaa gtgcagaaaa tcggcgtttt ttaaaaacac
    25021 agccgattgt tctttactta gaggcagaag cggactgtct agttgatcgt atccaacaag
    25081 atgaaacgtc tattcgacct ttagctcttg gaaaaacaag agaagaaata aaagcattat
    25141 tggcacaacg actttcttgg tatgaagaaa gtgcgacaca tcgaatcaac acgaccaatc
    25201 aaacaccaga aaagatcatc gataccatta tagaaaggat caaaagcata tgaacgtcag
    25261 ctatctaggg ccagaaagtt cattcactta tcaggcggct tgtcagctat ttcctacaga
    25321 acaattgact gcgtatgctt ccatccctgc ttgtttgcgc gctttgtttc gcaaacaagt
    25381 cgatctggct gtcgttcctg tagagaattc attggaagga tctgttcatc acacaatcga
    25441 tcttctttcc aaacatccag aagtcgaagt taaaagtgaa atcgttcttc ctatcaagca
    25501 gcagctttta ggcaatccag caacaaagat tacaaaaatc ctttcacatc cacaagcact
    25561 agcacaatca caacagtttt tagaaacgca ttatcctaat gttcctttag tagcaacaga
    25621 atcaacaact gctgcagcaa tgtatgtagc agaacatccg aaagaagacg cagcagcgat
    25681 tgcttcatta gaaacagcac agcatgttgg attggagatc ttagctgaga atatccaaga
    25741 taatgagttg aatcagacac gtttttggat cataggtgat cgaaagatga ccagccagca
    25801 gccagctcca gtaaaaatga gtgtcatctt gacactacct gctaatcggc cagggatgct
    25861 tcataaaatg ctggcagctt tcggatggcg ggagatcaac ttaagtaaga ttgaatcccg
    25921 accattgaaa accagtttgg gagaatattt tttcgtgatc gatttgttgt tggaccgccc
    25981 aatgacatta gtagagaatg cgctccaaga aatcaagatg ctcggaggag aaagtcaaat
    26041 attgggctgt tatccagtgc tgacagtaga agagacgcgt agatgaaagt caagaatact
    26101 gattgattta tttaacttaa aataaaaaaa tcagtgcttg gctattcttt atttttgata
    26161 ttctgattga tttttaggaa gaagcggtag acaaatcgtt acagtcgttc ctgtcccaat
    26221 cactgattgg atcgttaagc catatgcatc gccatacatt aggcgtatcc gctcatctac
    26281 atggctgatg ccgattcctg aaaaatgatc atttttttgt ttggtatttt cagtatcgaa
    26341 gccatcgccg ttatcaatca cttcgaataa aagaagctga ttttttgttt tgccgtacac
    26401 cgtgatgaat ccttgcgttt tattgtgaaa agcatgaaga taggcgtttt cgataattgg
    26461 ttgaaggatc atattcggca tagataatga ctgcagttca tcaggaatca ttattttagc
    26521 cgagattctt tcgccatatc gcatactcaa aagatcgatg taactttgta cgcttttgat
    26581 ttcttccttg actggaatca cttcttcttt tttcccaatc gtacttctta gtaaagtaat
    26641 gaaactgtcg attccttgaa ctgcttcatc tgtttttttc tgccaaatca aaaatttcaa
    26701 gctagtcagc gtattataaa taaaatgggg ctgtatctgt gcttgcatag tttggatttc
    26761 aagcgaacgt ttcttctttt cgtcttggat cgtctgttca taatgatgct gagcattact
    26821 tagcaattga ttgatcgtat tccctagcaa ttggacttca tggcttcctt gtacaggaac
    26881 agtagctttt aagccatctt cctctagccg ttttagcgaa tcaatcaagc ggtagatcga
    26941 ttttgtgtgt cgatccaaat acataaatac caatgagcca gcaaaagcaa gtgtcacgat
    27001 attatacaaa ataatcggaa aaatcacgcc catttggtta gataaagaat gaagatcgac
    27061 ttgactgagt aatgtacaat cctggcggta cagaggaaga cgggtcaact gctgtccatt
    27121 tgaaatcgtt gtttcaccta ctgttagttt tttgaaaatc attgatcggc tcccaattgt
    27181 attttcttca tttgatgcga tgatcgtctg ttcttcgttc atgaaggaga tcttcgatat
    27241 ctcaggattt actacagatt gaaaaagatt cgctagttgc ttggatgaga tcgataaaac
    27301 agcatatccg taaacgtgat tcaatgtatt tttcagtttt ttgacaaaga agacgctggg
    27361 tgctttctta tcgcgaagag tcaatccgtg gtgtgcaaaa agcatttgtg tatcagcagc
    27421 acgttcattc actctctgag caatcgcaga ttgaaaaaaa gaatcggcag atacggcagt
    27481 gagcatgtcg tttccgacaa aagttcttcc attttctcca aagatcaata catcgtaatc
    27541 gatcattgga tggatttttg aaagagagtc attcattttt tggattgttc ttagttgctg
    27601 aagctgttcc atatgttggg ctggatcatt ggaaaaatag cttttgacag ctggattaga
    27661 atcaaaagcg gctaatgtat tctcgattgt atcattcaaa ctttcaaatt cagattgaat
    27721 cttttttagt accacatcat tcgattgatt gaacagctgg atataggcat cccttgatcg
    27781 atgataaata aacaaagaaa tgacgatggc ttgcagaaaa agaccgatga acaaaatgaa
    27841 cagaagtttg gcaaaaagtt catgctcttt gatttttttt ctcattttac aaaagttcct
    27901 ttacggtatt gagaaggtgt cattcctgtc tcttctttaa aaactctgga gaaataaccc
    27961 agttcggtat agccgacttt ctcacagatt tccgtcaaag taagccgtgt ttccgtcaaa
    28021 aagagcttgg ctttactgat acggactttt tttagatatt ttgaaaagtt gataccaaat
    28081 ttttcagtaa agatggctga gagatagcta tatgaaaaat gatatttttc ggataaaagt
    28141 tgtaaattga tttctttctc ataattttcc tggacgaact cgtagatcaa agtcaagata
    28201 tcgaaattgt ctttttttgc atttgcagaa aagtgggtga gtttctcaag tgtttctttg
    28261 acaagaatag aaaaatcttc gaaatactca gcatcggcta ataaagaagg aagggcgatc
    28321 ttgagcaaag aaagttcttc attcccaaca tgctcgtcag ccaaagcgtt gagtaatgca
    28381 tagaaacgat tgattgcctc atgtttcaaa taagctggat caataggctg ttccaatatc
    28441 gtcgtgaaga aattctcaag ttctttgaca gctaaagcgt agtctttatg aacgattttt
    28501 gttaaataac tacttgcttg ctgctcgtgg ttggttaata acggaagaag ttcatgctga
    28561 taagcaaggt tctttccctt atgatagaaa cgttgtcctt tggtgcaatt tctgatctct
    28621 tggaatattt cattgatatc ttggattgca aaagaatcag aaacaacaaa aaaatcacct
    28681 ggaaaaactt cttgacgaaa cttttgaaca aacttcctaa gtgtatcttt ggaatcagta
    28741 ttgattacat agccggattc ttccgaaaag gtagaaacag gataaatagt aaacgaatcg
    28801 tcgctatcca tcaatgaatg ggtgatcgac tgtttttgtt tagagccttg ttttgaactg
    28861 aatggccgtt tttcggtata taacagaaaa aacaaggtgt gttcaaaaag aagagggtca
    28921 agcaatggac gagtttgtcc agcaatctgc tgtgaaagat tctcgcagat ctgctcataa
    28981 cgattggtga ttgggctttt gttgggcttt tttttgattt tttgtgaaac ggcagttaag
    29041 gaatttagaa aaaaatcagg actcaatcga ggctttaaaa gataatcagc gacgccttct
    29101 ttaaaagaat ctttaaccat atcaaattca tcatagctag agaggataag gatctgtgtt
    29161 tcaggttgtt gctgttttaa tagccgcgat aattcgattc catttaattc aggcatcatt
    29221 acatctgtga ccacgatatc gattgcatgt accaaagaaa aatcaagcgc tttcaacgga
    29281 ctggaaaaag tccctattaa ttgaaacccg tgctcagacc aattgatcaa ctgtttcatt
    29341 ccatctaaaa tcgtctgttc atcatcgata agaattacgc gtattggcat ttttttaccc
    29401 gccttaagaa tagtcttgta atttaaaaat acttatttct aggagaaaat gcaaggatat
    29461 ttttatttta aagaaacctc accatttcga tctttggtct agatgctaag attattcctt
    29521 ttgtcatagt tgatttgtat ttttttatta ttttaggaga aatttttgaa gtatctgaat
    29581 aataaagaaa ccgttttcaa attttggtat gatgaatgta gcaaacgtaa acaactaatg
    29641 gatggaaaag gagcggataa aaatgaacat cagaaagtta attgctggag tcgtttgttt
    29701 agcggcagca tcaggtttct taacaggatg cggaagtgct agtagtacag cagacaaaga
    29761 agaaaaagtc actgtctggg cgtgggatga gacattcaat atcaaagcag tgaatgaagc
    29821 aaaaaaagtt tatgagaacg aagaaacaga gatagaagtt gttacgatgt ctcaggatga
    29881 tatcgttcag aaactgaaca ccgctttagc gagcgggaat acagaaggat tgcccaatat
    29941 cgtcttgatt gaagattata ggatacaagg ctatttgact tcttatccag atgcattcgc
    30001 taatttgtct gctattgtaa aggaagatca ttttgcaccg tataaatttg cggtaaacaa
    30061 agtaggagat aaaatatacg gtgttccttt cgattcgggc gtaacagcaa acttctatcg
    30121 tacggatctg atggcagaag caggttatac agaagaggat atggagaatc tgaagtggga
    30181 cgagtatatt caagtagcgc gagatgtcaa agaaaagacg ggaaagaaaa tcgctgaagt
    30241 caatccttct gaccttggtc gagtaaggat gattatgcaa caggcaggag aatggtatac
    30301 atctgaagat ggggagacag tcacgatcca agacaatgca tctttaaagt atggtcttga
    30361 attgtttgcc actcttttga aagaagatct agtcgaacaa acctctgact ggaatgctgg
    30421 agtaactgcg atacaatcag gagcagtagc ttctagccca actggcgctt ggtattcttc
    30481 aacgatccaa ggagcagaaa accaatctgg aaaatggaag attgcaccaa tacctgcact
    30541 tccagaaaat ctgcaaaaag cgcaagcttc taaccttggc ggcgctggct ggtatgtgat
    30601 caaaggagta gctggagaat attcagcgaa agatttccta gaagcaacgt ttgcttcgaa
    30661 tgaagaactg atgggcactc ttgcgaaaga aatcggacta gtatctacga tgcttagcgc
    30721 gaatgaacaa gaagcttatc aagaagcttc tgaattttac agtggtcaaa agatctttga
    30781 cgatttttca acatggacat ctgaaatacc agaagtgaat tacggacatg agacctatgc
    30841 aattgaagca gtcgtagctg aagctcttca tcgaatcatc aatggagaag agacagacaa
    30901 ggtactagca gatacacaaa aacaagtaga agcacaattg gctaattgat cggtggtaaa
    30961 aaagtgaatt tcttcgcttt ttcatcagac aaacgaaaag acaaaaaaga tcacgtctgc
    31021 cttacagata ttgtctttct tgtcttttca tgtagataat caatgataga attggaggga
    31081 aaagaatgaa gcagaaagat ttattcaaaa acgggcatgc ttttttaatc ttgccagtca
    31141 ttttgatatc aatcatggtt tttattccga tggttttggc tttgatcacg tcttttcaaa
    31201 gtggtccccc tgtcaatatg acatttaacg ggttaggtaa ttatcagcgg atgttgacag
    31261 atgcaacttt taaaaaagct ttttttaata cattcctgta tttacttgta caagtaccga
    31321 tcatgttgtt tctagcactt tttatttcca accttttaaa tgataaaaag ttgaaatata
    31381 aagggttgtt tcgaaccgct atctttcttc catgtatcac ttcgatggtc agttattctc
    31441 tgatcatgaa aagtctattt tctcaatctg gccttgtaaa caacttgttg ttgggcattg
    31501 gtatgattga cacaccgatt ggctggttga ctcatccaat ttgggcgaga gttctgatca
    31561 ttctttcgat cacttggcgt tggactggat acaatatgat ttttttcctt tcaggcatgc
    31621 aaaatattga ttcggagatt tatgaagcag catctattga cggtgcttct aaatggcagc
    31681 agtttatcaa agtgacgatc ccaaatttaa aaccaatcat tttgtttact tccattactt
    31741 cgacgatcgg gacattgcag ctttttgatg aagtacaaaa tattactggc gggggaccag
    31801 caaatgcaac gaccacccta tctcaataca tctataattt gagttataaa ttcacgccta
    31861 attttggata tgcggcagca gtttcctttg ttattgtttc ctttattgtg atattgtcga
    31921 taatccaaac gaaagtagga ggagaaagtc gatgaaaaat atggtagaaa aagaaacaat
    31981 cgaacgcccc aacgtcaaaa aacaagcatt agaacttcgt tcgacaattg ggtcagtatt
    32041 aaagtataca gcattaagca tcctatcatt tatttccatt ttcccttttg tctggatggt
    32101 tttgggaatg acgaatacac ccgtggatat cacatcggga aaaataaaga ttggcagtaa
    32161 cttgatcatc aattttcaaa atttgttttc caatgagctg aattttactc gttccctttg
    32221 gaattcagcg atgatcgctt tggtgaccac tgtgtttgca ttgctgattt cgtcaatgtc
    32281 aggatatgga tttgagattt atcaaagtaa acgaaaagac ctaattttca atattctact
    32341 gctatcgatg atggttcctt ttgcggcatt gatgatccct ttatatcgaa tgttttcaca
    32401 attgaacgga acggttttgg gaatcaacag tttcttggtt gtcatcctgc catctgttag
    32461 cactgctttt ttgatcttct tctttcgaca aaatgttaaa tcttttccaa aagatctagt
    32521 agaagccgct cgattagacg gtctaaaaga actaagtatt ttttttagag tgtatatgcc
    32581 gacggctaaa aatacctatg ccgcggctgc aatcatcaca ttcatgagta gctggaacaa
    32641 ttatttatgg cccttagtag ctttgcaatc tcctgataaa cggacggttc cgcttgtttt
    32701 atctgctatg ggtgcttcct atacaccaga ctatggaatg atcatggctg gattagtcat
    32761 cgctacttta ccaactgcca tcgtattttt tgttttgcaa aagcaattcg ttcaaggaat
    32821 gatcggctct gtaaaataaa aaaacacaat aagcgcgttc cttcaaagtc atttttccaa
    32881 gtaaaaagat acttttggga cgtgtgttat tttatgttga gaaaaattaa atgataacgt
    32941 tttaagtttg tgctcgaaaa ctctttcatt ttaaggtaaa cttaaagagt aaaggaggag
    33001 gatacatgct aaaaaatttt aaagaagtga ttgctgctat attgccgatg actgtcctga
    33061 ttgtcatttt aacgtttgtt tttgcaccgt tagaaggaga agacctcgct tcatttctgg
    33121 ttggtgcggc aatcatgatg atcggaatga cgctcttttt gtttggagca gattattcta
    33181 tgatggaagt cggagatcta gtagggaaat atatgatcaa aaagaaaagt ttgactgttt
    33241 tggtcagttt gggatttgct atcgggatcg tgatcacgat tgctgaacct tccgtgcaag
    33301 ttttgggaca acaagtatat gatatatctg acggagagat tggcagagtg ttgctgattg
    33361 ggatcgtcag cgtcggcact ggagtatttc tagcctttgc gctgcttcgg gtcgtattca
    33421 agctatccta ttatcaattg atggctatag gctacatagg cgttttgatc gcctcatttt
    33481 tcaccagttc cgaatttatg ccgattgctt ttgattcggg gggagtgact actggtccag
    33541 taactgttcc atttatcttg gcccttgccg gagggatgac tagtatgatc aaacaaaaga
    33601 aaaatgaaaa tgacagtttt ggaatggtcg gtattgcttc gttagggccg attcttgccg
    33661 tgatgatttt gggggtgatc ttccaatgag tttctggcag caagtgatag atggaatcga
    33721 aatcgttacc atggaagtca ttttggcagt ggcacctatc gtaatcctat ttgttttaat
    33781 gaatatcatt agttttcgta tgcgcaaaaa gcgtttcttt gacattatga aaggtttctt
    33841 gataacgact attgggttga tccttttcct gcacggagtc aatatggctt atgtaccggt
    33901 aggacagcat ttaggcagtg ctattgcggg attgccttac aattatatac tggttcccct
    33961 tggcttttta atggggttct tagtagggtt tgctgagccg gcgattcatg taatggtcaa
    34021 acaggttgaa gaattgagcg aggggagaat ccgatcgaaa atcatgctga tggtaatttc
    34081 aattgggatc ggcgcagcag taggattgtc tatgtggcgt ttgctcgtcg gattctctct
    34141 ttattatttc ttgatcccag gatacgggct agcatttatt ttaggacgaa aagtggacaa
    34201 gatcttttta gcaatggctt ttgataacgg tggagtagca acgggcccga tgtgttcgac
    34261 atttatttta tcaatgagcg tggcaatcgc tgcacaaatt gaaggaagag atccgatcat
    34321 tgacgggttt ggtgtggttg caatgattgc attgacacca atattatcca ctttagtact
    34381 aggatatatt tataaacgaa aagatgaaaa agacagaatc gaaaaagaaa gattgcaaaa
    34441 attgggggaa agcaaatgaa agatcttgta gcattgaatc ttcacatgat tgtgacgatc
    34501 gttgacggag gaatgggcga agatgttatc gacttttcaa aagaagcagg tgcagcaggc
    34561 ggaacgatct tacatggaag aggttctggt gtacatgatt cagggaaatt tttaggtttg
    34621 gacatttcgc cagaaaaaga tattgtgttg acattagtac ccgatagttt gacgaataaa
    34681 gtaatggatg cgattggtca aggcatcaac atcgatattc caggtaatgg tatttgtttt
    34741 agtattgatg tagacaaagt cattggtatc actaagattc atcaatatcg agaaattatc
    34801 ggaatgcaag aacttttaga tgaaaataac gatagagaaa agtctgaatc atagacaagc
    34861 gtttgaagag gaaataaaca caaacaagaa ggcggatgag cgttatttcc gcttttttgt
    34921 ttgtattttt ctcgtgaata cttatgaaat agtaaatttt tatgaaattc tactctaagc
    34981 atcagacttt cttttcaaaa tatgctattt taaaaatgat gaaaaaaggg ggagaaaaat
    35041 gaatttttta gttgttttaa aagatactct gattgagcga tctacatttc tatatgttca
    35101 attgcaggaa aataaaacat tgctgcattt ttcaccagag ttccatctag aggggctaac
    35161 attaggagaa atctatcaag cagaagggct gtcggctgtt ttgcactttt ttaaggtaga
    35221 attggaatta ccagtaaaag attatatcga gctttctctt gaatcttggg atcgagcagt
    35281 ggttgaactg tttccagaag gtttaatgat cgatacaaac gatgggaaga tccatctgac
    35341 tgcagcagaa ctgcaaaaac aaatgcgtta tcaaatagat gacgaaaatt ctttttctat
    35401 ttttagacgg cagcaaaaga ttctaaaaag ctttttaaaa gtgatcagta aaaaacggaa
    35461 tctgatcaaa acgacacagc tgttgaaaaa atatccaggt ctgcttcaca cgtctattca
    35521 atttcctcaa ttgattacga tgatccatcg ttttatacgt atgcaacagc taccatctaa
    35581 gaagctgttt cttccattga cggacacatt tcgagtagtc aacagagaag ataaaaagaa
    35641 agtgattgtg attgacttta ccagaaatcg gaatgtcctt caccaagttg caatggaaaa
    35701 ggccaattaa ggctttttct ttttggattt ttttgtcata ataaatatta gatgatggag
    35761 gagataatat gatgcaaaca tggttagaaa ggctaatacg atttcagcag aactatcaca
    35821 aacttatgca gggaagatat gcgaaatttg atcagttgaa tcgcggtcta ttgatcgctg
    35881 cgcttgtctt tagtttgcta agtcgttggt tgccctatag aatcggacaa ttgttgttca
    35941 tcgtcttttt cggatggatg atatttcgtt tcctatcaaa aaaaatctac cccagactaa
    36001 atgagaatca acgttatttg aaatatcttg atagagtcaa agaaaaatat catcagataa
    36061 aaaacaaaaa gcaagtcaaa atggatcgaa aaagttatac tttttttgag tgcccgaatt
    36121 gtcatcaaaa gcagcgtgca cctaaaggaa aaggacgaat acgtgtgaca tgcaaaacat
    36181 gtggtataaa attcgaaacg aatgtttaag ggagaaaaga atgaaattag tattattacg
    36241 tcatggcgaa agcgaagcaa attttgaaaa ttactggaca ggatggctag atgtcgcctt
    36301 aacagaaaaa ggaaaagaac aagcagcagt tgccggtcaa aaaatgcttg aagccggact
    36361 tcaattcgat gccgtttata cctctgtttt gaaacgtgcg attcttaccg gacaagtcgc
    36421 actagaagaa atgggtcagt tatggcttcc tataataaaa acatggcgtc taaatgaacg
    36481 gcattatggg gatcttgtgg gtaaaaacaa agataagatg aaagaaatat acggaaaaga
    36541 tcaagtaaaa acatggcgca gaggatatac ggaagttccg ccgttaaccg cagaaaatag
    36601 atttgaccgc agatatgatc acttggatcc gcatttgatc ccatacggag aaagtctgga
    36661 aatgacagtg aatcggatca ttcccttatg gcaagatcat ctagcaccaa aactaaaaga
    36721 tcggcaagat ttactagttg tgggtcatgg taatagcatc cgagcactga caaaatattt
    36781 agaagatgtt cctgaagatc aaatggatac gatcgatatc ccaaatgcac aaccaatcca
    36841 gtatacatta gcagatgatt tgacaattct ggacaaaaaa atactttaaa gaacaccttt
    36901 atctaaaagt gagagataga aaaaagaagc aaaggaatgg aacaaaagga acatgaaaaa
    36961 ttatatatgg gactttgatg gtacattgtt tgatacgtat cccgcaatgg tagatggtgc
    37021 atggcaagca ctaaaagatt ttggtatcag tatggacaaa aaagaaatct acttcaaaat
    37081 gaaaaagtat tctactagtt atttgatcaa tgaatcaaac ttgaatgcca gagagtttaa
    37141 tgaattattt caccgatatg aaaaagaaag tacggaagtt tcccgtccat ttccagaaac
    37201 gaaacaagta ctggaaatgc taaaagacaa cggcggtcgt catttcatcc tgacccaccg
    37261 tttgacagaa tcaacatggg gcctgttaaa agaacatcga ttggcacatc tgattgagga
    37321 agtagtaggg atcgatcagg actttcctcg taaacctgat ccagcatctt taaattattt
    37381 gatcgatact tttcatttag aaagaacaga cacgatgatg atcggcgatc gtcgtttaga
    37441 tatcgaagca gggaaaaatg caggagtcgc tacttgtctt tacgacatcg atcattttct
    37501 gggtgagatc ccggcagatt atgtggtagg aaatctgaat gaaatcctta ccttgcagag
    37561 catcattaaa aagtagtatc ccctttcaaa ggagatatgt tataattttc ataaaggagt
    37621 gataaaatta tatggaaatc agaagacaaa aagaagttgt agaagcattt cattatgata
    37681 tgagattgcc gaaccaagaa gtagaaacag atttgaaagt aggcttctcg cctttaaaag
    37741 aaacgatcga aaactatccg aaagaacata gcatcgtagg agcacgctta gagttccgtt
    37801 tagtgtttga tacatatgtt ttatctggaa gtgtgagtca gatcaatcat atcctcaatc
    37861 ggaaaattga aggtcaaaat gatgtcacac aagaagaagt ggacgagcta gtagcaccgt
    37921 tattcgatat cgtacgccga atgtcttatg aggtgacaga gatcgcctta gatcagcctg
    37981 gggtaaaatt gaattttcaa tcagaaaaac aagaatagac agtaaaaaca tacctgcgat
    38041 agattaaagc tatcacaggt atgttttttt attactaatt gtaagcaaaa agaatgtcat
    38101 ttcgataaaa atcgtctaaa atataaaagt cactgaaaaa aattttttca ggaggatggt
    38161 tgaagaatca ttctcgggaa cctttacttc cttcgatgga cttgtcatta ataatatgag
    38221 gaggagaaaa atgacaacac tcatcacagt tttagtggtt ctagtttttg cggcattatt
    38281 atatgtgttc tatcgtatgc aaaagcgcca tacaaaattt tcaacacgcg tctttacagc
    38341 attaggagtt ggtatcgtct ttggcggtct gcttcaattg atctttggtg tagacgacaa
    38401 agtcgttact caatcaatgg aatggatcgg gattgtaggt caaggatatg tatcattctt
    38461 gcaaatgcta gttatgcctt tagtgtttat ttcgatcatt ggtgcattca caaaaatgaa
    38521 agtttctgaa aagctaggga aaattagtgc gactgtattg acttctttac ttggaacaac
    38581 agctattgca gcattgatcg gaattggttc aacaatgctg ttcggactcc aaggagctaa
    38641 attcacacaa ggagctgcag aaacagcacg aatctctgaa ctggcaacac gtcaggaaac
    38701 agttgaaaac ttatcgattc cacagcagat tttatccttt atccctaaaa acgtttttgc
    38761 tgatttagca ggtagtcgtg caacaagtac gattggagtc gttattttcg cggcatttgt
    38821 cggaatcgct tatctaggcg tgaaaagaaa agcgccaaaa gaagcagcat tcttttcaga
    38881 tttagtcgac agtctgtata aaatcattat gcggatcgtg acgcttgttt tgcgtttgac
    38941 accttatgga gtatttgctt tgatgacaaa cgttttggca acaagtgatt ttgcagctat
    39001 cgtcaattta ggtaaatttg tcgttgcttc ttacgtagca ttgcttgctg ttttgattgt
    39061 ccactcactg attttgattg gtgcaaaagt caatccaatc aactatttca aaaaagcctt
    39121 tccggtattg agctttgcgt ttacttcacg ttcaagtgct ggagctttgc cattgaatat
    39181 cgagactcag acaaaagctt taggagtaga tgatgcaaca gctaatttct cagcaagttt
    39241 tggtttgtct attggacaaa acggctgtgc tggtgtttat ccagctatgt tggcaacgat
    39301 cgtcgcacca gcagctggag tgaatgtctt tagtttggaa tttattttga tgcttgtggc
    39361 aattgtaaca atcagttctt ttggtgtagc tggagtcggt gggggcgcaa catttgcttc
    39421 actgatcgtt ttaggggcaa tgaacttgcc agtagcaatc gttgggttag ttatctctgt
    39481 tgaaccatta atcgatatgg cacgtacagc agtaaacgtc agcggtagca tggtagctgg
    39541 tatcgtgaca agtgcacgca tcaaagattt agatcgcgat gtcatcaatg atgattctaa
    39601 agtgatcgat gtgaatgcat agtcaaaaag aggttgtgat agaagtggtc agctttaaga
    39661 aataagaaga agtttcctaa aaatagtttc tcaaattttt gtgaaatttc ggcttatttc
    39721 cgagaagcta cttctgaaac accgtgaata agaggttgtg aaaaagctgc cctgcatcaa
    39781 gtaataagaa cagccaaaca aaaatagttc ctcatatttt acgtttggtt gagcttatta
    39841 ccgcagatgc aagcttttga acaccgtttt atttgtctca cacccttttc cttcctactt
    39901 gtaaccttgc tcccgtgtta gaaacatgtt acactaaata tgtgaaatta ttaacaagga
    39961 gcgtcgttat gcgagaaaaa acagatacac tgccaaaagc aactgcgaaa agattacctt
    40021 tatatttacg ctacttgaag atgctggatg actcaggtat aagtagaatc aaatcaaatg
    40081 agttcagcga aatcacccaa attccttctg caacgatccg cagagacttc tcgcaattag
    40141 gcgagctagg cagaagcgga tatggctatg atgtcccttt tcttatcgat gtattcaata
    40201 atatccttaa tactaaagaa gaaaaacgaa tcgcattagt aggatatgga aatcttggca
    40261 aagcattgaa acacaacaat tttagaagaa atgaaaatct aaatatcgtg tgcgtctttg
    40321 ataatgatcc agcactgatc aatcgagtaa ttgatggaga aatgatctat ccgattgatc
    40381 gtttcgcgga gatagccaaa gcaaaaaatg tgacagtagc aatctctacg gtaccaagta
    40441 aatattcaca atctgctatt gatgaaattg tcaaaggaaa tgtaaccgct atacttaatt
    40501 ttgcgcctga cagagtaact gtcccagctt atgtcaatgt tcaatacatt gatttgacaa
    40561 ccgagttgca gacgcttatt tactttgatg agaattactc agaagttttt tcttaggaaa
    40621 atttgagtag gcttattgat cttgcgagaa ctcgtcgtaa ttctttcaaa aattttttag
    40681 tactaaatga agtggttttt tcacgaccac ttgttgacta aaaaaaaaaa ccattgtaga
    40741 atgaagcata acgctgaaca agaggagtaa ctggcacatt cattcttaga gagagaacta
    40801 tctgctgaaa ggttctcaat gacaaccagt gaaggtagct tgggagtttc tgtcttgaat
    40861 ggagtagaga tagacgagtc gtgatcgtta actcacttga gaggcaataa tgattgatta
    40921 ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga atacgaaagt attttaggac
    40981 gttttttagt ttaaaggagg aaaacagcat gacggaagaa aataacctat caacaaaata
    41041 taatccgcaa gaagttgaag ctggacgtta tcagcattgg ttagaacaag atttgtttaa
    41101 accgagcgga gataaaaaag caaaacctta ttcaatcgta atcccgcccc caaatgttac
    41161 tggaaaactt catttagggc atgcttggga tacgaccttg caagatatgc tgattcgtca
    41221 aaagagaatg caaggatatg atactttatg gctgcctggt atggaccatg caggaatcgc
    41281 gacacaagca aaagtagaag cgaagcttag agagcaaggg atttctcgtt atgatctagg
    41341 tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa gagtatgcga gccacatccg
    41401 cgaacagtgg gctaaaatgg gcttatcttt agattacagt agggaacgtt ttacattaga
    41461 tgatggatta tctgaagcag tacgtaaagt ttttgttacc ttatacgaaa aaggtttgat
    41521 ttatcgtggt gaatatatca tcaactggga tcctcaagca cgaacagctc tatctgatat
    41581 cgaagttatt cataaagata tcgaaggcgc cttttatcat atgagctatg aactagcaga
    41641 tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa acgatgttgg gagatacggc
    41701 gattgctgtt catccagaag acgagcgtta tcaagcgctc atcggcaaaa aagtgattct
    41761 acctttagtc aataaacaaa tcccaatcat tgcagatgaa tatgtagata tggaattcgg
    41821 gacaggtgta gtaaaaatca ctccagcaca tgatccaaac gactttgagg taggtaatcg
    41881 tcatgatctt ccacgcgtaa atgttatgga tgatgatggt acgatgaatg aactagcagg
    41941 aaaatatgct ggaatggaac gttttgctgc tcgtaaagca atcgtgcaag atctgaaaga
    42001 aatcggtcga ttgattaaaa tcgatacgat gatccacagt gtgggtcatt ccgagcggac
    42061 aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt gtaaaaatgg cgccgttagc
    42121 gaaaaaagcc attgataacc aagaaacaga tcaagcagtg gaattcttcc ctccaagatt
    42181 caatcaaacg tttttacgtt ggatggaaaa tgtccatgat tgggtaatct cacgacaatt
    42241 atggtgggga catcaaattc ctgcatggta tcacaaagaa acaggtgaaa tgtatgtagg
    42301 gatggaagca ccagcggata gtgaaaactg ggtacaagat cctgatgttt tagacacttg
    42361 gtttagttca gctttgtggc ctttctcaac aatgggctgg ccagatacgg aaagcgaaga
    42421 ttataagcga tacttcccta ctagcacttt agtaaccggt tatgacatca ttttcttctg
    42481 ggtaagcaga atgattttcc aaagcttgga atttactggc gaacgtccat ttgagaatgt
    42541 cttgattcat ggattgattc gtgacgaaca agggcggaag atgagtaaat cattaggtaa
    42601 tggaatcgat ccgatggaag taatcgaaaa gtatggagca gatgctctcc gctggttctt
    42661 atcgaatggt tctgcaccag gacaagatgt ccgcttcagc tatgaaaaaa tggatgcttc
    42721 ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc gttatcatga atatcgaagg
    42781 aatgaccgta gaagagatcg atttatctgg agaaaaatca gtggctgacc ggtggatcct
    42841 tactcgtttg aatgaaacga tcgaacgagt aactgagcta tttgatcgtt ttgaatttgg
    42901 tgaagcaggt cgacaactgt ataactttat ctgggatgat ttttgtgact ggtacatcga
    42961 aatgagtaaa gaagtacttt atggagatga tgctgtttca aaacaaacca cccgtagcat
    43021 cttagtccat acacttgatc agatcctacg tttattgcat ccaatcatgc cattcgttac
    43081 agaagaaata tgggaacata ttcctcatca gggaaattct ctagtagtgg cagaatatcc
    43141 tgttgtacgt ccagagtttg atgatgaaac agcttctaaa gggatggaag tattgaaaga
    43201 attgattcga gccgttcgaa atattcgttc agaagtgaat acaccattgt ctaaaccaat
    43261 tacgttgcta attaaaacaa acgatgctaa aatcgatcaa tttttagttg aaaatacaag
    43321 ctatatcgaa cgcttctgta atcccgaaga gttgacgata tcaagtgaga tcactgcgcc
    43381 agatctagcg atgtcagctg ttctgacagg tgcagaaatc ttcttgccgt tagcaggatt
    43441 gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa ctggaaaagt ggacaagcga
    43501 agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt gtttctaacg cgccggaaga
    43561 agtagtagag gcagagcgtg caaaagaaaa agattattta gagaagcaaa cggctgtcaa
    43621 agaacgaatc gaaagtttgc gtactattca ataagaatag aaaaagagag tgtgaaatca
    43681 gctagctatt actaaaagct agctgattga acatcgttac aggaaagagt ttgagcctca
    43741 ctcttagctt cgattcataa ggagatgtga cataaatttt gtcgcatctc ttttttagtc
    43801 tactcttatt atgtaggaga aggagggagg tggatcatgt atcaagagtc aataaagtag
    43861 ttgataaaat cgttcctctc tctgtttctt ttctctttgt catactttta tttttcttat
    43921 gtgttctatt tgttataata aaagagaaat gggggaattg ttgatgacga ttgaagaagc
    43981 aatagaatgg atccatagtc gattaccatt tggttcacgt cctggccttg accgagtcga
    44041 agctttatta gaagtggtag ggcatccaga gagaaaagta aaaacgatcc atatcgctgg
    44101 aacaaacgga aaaggttcta ctgtagctta tttgagaagc ctattagaag aggctgggct
    44161 gacagtaggt acatttactt caccttatat cgaaacgttc aatgaacgga tcgcaatcaa
    44221 tggcaaaagc attccagaca atgatttgat ccagcttgta aaaaaatatc agccactgat
    44281 caaacaactt gatcaatttg aagcagtagc tggtattact gaattcgaga cattaacagc
    44341 aatggcattt gattattttt tgatgaagaa agtggacgta gcgattatcg aagttggctt
    44401 aggcggctta ttggattcta ccaatgtagc tgtaccaatg ctgacgggaa taacaacgat
    44461 tggacttgat cataccgaaa tacttggaga tacaattgaa gaaatcgctg cacaaaaagc
    44521 tggaatcatc aaacaaggcg ttccagtagt aacaggaaat attgccccag aagcttttac
    44581 tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt tatgtatacg gggaaaatta
    44641 taaaataaac tatctgcatc ctgatgagat gtggggagaa gtatttgaat tttttaatga
    44701 aataggaaag atccctcaat taaccactcc gcttttaggg caacatcaag ctgaaaatgc
    44761 aggagtagcc atcgaattgt attttgttta ctgtcaattg gaaaagctgc ctttccgaga
    44821 aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca ggaagaatgg agcgtttgag
    44881 taacgagcca ttagttgttt tagatggtgc ccataatacg catgcaatga accggcttgt
    44941 tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac attttgtttt ccgccttacg
    45001 aactaaaaat gttagagaaa tgctagaacg attgcagcaa gtaccaaatg tccatattta
    45061 tttgacgacc tttgaacatc caaaagccat cgaattaagt gaagtatcag atttagcaag
    45121 tgagagagta tcaatcgttt cattgtggca attcggatta gcagatatat tggaaaagat
    45181 gacaaatgaa gacgtattag ttgtgaccgg ttcactttat ttcatttcag aagttcgcca
    45241 tttattaata caattaggag gagatcatga agtataatgc agtgattttt gacatggatg
    45301 gtttgctgtt cgacacagag ttagtgtatt acgaagccac acaagtagta gcagacaaga
    45361 tgggttttcc ttatgataaa gacctgtatt taaaattctt aggtgtttca gatgaagaag
    45421 tatgggcgaa ttatcatatg attttctcgg aatttggcaa aacaagtgta caaagattca
    45481 tagatgattc ataccaggaa acgcttgaac gtttttcgca aggagcggta caaatgaagc
    45541 ccggtgtgtt ggaactcctc tcttttttag aagaacatca gattcccaaa gttgtcgctt
    45601 ccagtaacca acgagcagtc attgaacttt tattgaagaa gaacaatctg tttgaacgat
    45661 ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa accagaccca gaaattttct
    45721 tgttagctaa tcagaagcta ggtacagcga agaaagaaac attgatttta gaagattcac
    45781 aaaatggtat tttagcagct tctgcggctg agatcccggt agtcatggta ccagatctgc
    45841 ttccaccatc ccaagaattg atcaaaaaaa cagctggggt tgtttcttct ttacatgaaa
    45901 tccctgcatt gttaaaataa aaatccatct cttttcgtat cttattttag ataagataga
    45961 aaggagatgt ttttttggga aaacgattga tcaaagaagt acctactagc tctttaccaa
    46021 gggaacgtat ggaaatttat ggcgcggaag ctttatcgga tcaggagctt ttggcaatct
    46081 tactgcgtac cggtcaacac ccttatagtg tgatgtcgat tgctggaaat ctgttaaaaa
    46141 cgtttggagg actggcctct ttacgacagg ctactttgca tgagttagaa gagattcgag
    46201 ggataggacg cgtgaaagca atcgaaatca aagccttgat cgaattaggt cgacgaattc
    46261 agacggatca tgaaagagcg gctccaccgg ttcgtaccag ctacgaattg gcccagcaat
    46321 tgattctgga aatgaaagat cacaaacaag aacatttggt ttgtatttat ttggacacga
    46381 agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc gctgaatcag tcagtcgctc
    46441 atcctagaga aattttccat tatgctgtac gttattgtgc agcaaggatc gttttagcgc
    46501 ataatcatcc ttctggtaat gttattccat cgcagcagga tatgaatttt accaaaagga
    46561 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    46621 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    46681 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    46741 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    46801 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    46861 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    46921 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    46981 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    47041 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    47101 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    47161 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    47221 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    47281 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    47341 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    47401 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    47461 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    47521 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    47581 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    47641 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    47701 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    47761 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    47821 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    47881 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    47941 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    48001 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    48061 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    48121 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    48181 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    48241 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    48301 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    48361 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    48421 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    48481 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    48541 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    48601 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    48661 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    48721 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    48781 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    48841 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    48901 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    48961 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    49021 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    49081 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    49141 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    49201 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    49261 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    49321 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    49381 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    49441 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    49501 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    49561 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    49621 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    49681 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    49741 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    49801 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    49861 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    49921 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    49981 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    50041 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    50101 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    50161 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    50221 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    50281 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    50341 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    50401 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    50461 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    50521 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    50581 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    50641 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    50701 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    50761 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    50821 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    50881 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    50941 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    51001 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    51061 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    51121 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    51181 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    51241 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    51301 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    51361 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    51421 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    51481 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    51541 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    51601 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    51661 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    51721 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    51781 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    51841 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    51901 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    51961 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    52021 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    52081 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    52141 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    52201 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    52261 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    52321 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    52381 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    52441 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    52501 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    52561 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    52621 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    52681 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    52741 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    52801 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    52861 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    52921 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    52981 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    53041 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    53101 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    53161 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    53221 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    53281 aagtgtacga attaatttta aaaaaagta
//
LOCUS       DAEGIQ010000008        51803 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000008 DAEGIQ010000000
VERSION     DAEGIQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.008"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..734
                     /locus_tag="KMT45_000410"
     CDS             <1..734
                     /locus_tag="KMT45_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003898.1"
                     /translation="LAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSF
                     GKPFFLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTY
                     VNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNK
                     KTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSV
                     QKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(974..1057)
                     /locus_tag="KMT45_000411"
     tRNA            complement(974..1057)
                     /locus_tag="KMT45_000411"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1021..1023),aa:Leu,seq:gag)
     gene            1112..1291
                     /locus_tag="KMT45_000412"
     CDS             1112..1291
                     /locus_tag="KMT45_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003899.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1440..2657
                     /locus_tag="KMT45_000413"
     CDS             1440..2657
                     /locus_tag="KMT45_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBE7003900.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2841..3677
                     /locus_tag="KMT45_000414"
     CDS             2841..3677
                     /locus_tag="KMT45_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7003901.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSQDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYFGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3753..4901
                     /gene="nagA"
                     /locus_tag="KMT45_000415"
     CDS             3753..4901
                     /gene="nagA"
                     /locus_tag="KMT45_000415"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBE7003902.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5122..6180
                     /locus_tag="KMT45_000416"
     CDS             5122..6180
                     /locus_tag="KMT45_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBE7003903.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6184..7218
                     /locus_tag="KMT45_000417"
     CDS             6184..7218
                     /locus_tag="KMT45_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBE7003904.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7320..8036)
                     /locus_tag="KMT45_000418"
     CDS             complement(7320..8036)
                     /locus_tag="KMT45_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBE7003905.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPDESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(8005..9585)
                     /locus_tag="KMT45_000419"
     CDS             complement(8005..9585)
                     /locus_tag="KMT45_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBE7003906.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYDQEIIGAVCV
                     GLTNNTIDNQVKDGQKMKKVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILL
                     INTPAKNIFYQTEQLLDFEVGKKISDNLYDVLFKETIEHQTTVRTQSMYLNGIETISS
                     VTPLFSNDRFLGAVATLRNRSEVMKLMEELGGTKQYINSMRAQTHEFMNKLHVISGLI
                     DLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVPAIAGFLLGKINEAKEQSVEMLLESD
                     SFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQKHERNGQVRIHLNYDTEAEVFIVTVC
                     DDGVGISEEISEKIFTLGFSTKGINRGFGLNLIHSIVKRHGGFLEFSKQTNGGTIVYL
                     ELPKKEDQNDDKCPDNRR"
     gene            9760..10284
                     /locus_tag="KMT45_000420"
     CDS             9760..10284
                     /locus_tag="KMT45_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBE7003907.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10300..11799
                     /locus_tag="KMT45_000421"
     CDS             10300..11799
                     /locus_tag="KMT45_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBE7003908.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11887..12489)
                     /locus_tag="KMT45_000422"
     CDS             complement(11887..12489)
                     /locus_tag="KMT45_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBE7003909.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12593..13549
                     /locus_tag="KMT45_000423"
     CDS             12593..13549
                     /locus_tag="KMT45_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBE7003910.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILARIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13854..14810)
                     /locus_tag="KMT45_000424"
     CDS             complement(13854..14810)
                     /locus_tag="KMT45_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE7003911.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNISE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14984..15544
                     /locus_tag="KMT45_000425"
     CDS             14984..15544
                     /locus_tag="KMT45_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003912.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15537..16361
                     /locus_tag="KMT45_000426"
     CDS             15537..16361
                     /locus_tag="KMT45_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBE7003913.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16371..16988
                     /locus_tag="KMT45_000427"
     CDS             16371..16988
                     /locus_tag="KMT45_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBE7003914.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17006..17689
                     /locus_tag="KMT45_000428"
     CDS             17006..17689
                     /locus_tag="KMT45_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE7003915.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPVIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18372..19655
                     /locus_tag="KMT45_000429"
     CDS             18372..19655
                     /locus_tag="KMT45_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE7003916.1"
                     /translation="MKKSMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19671..20558
                     /locus_tag="KMT45_000430"
     CDS             19671..20558
                     /locus_tag="KMT45_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7003917.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20575..21411
                     /locus_tag="KMT45_000431"
     CDS             20575..21411
                     /locus_tag="KMT45_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7003918.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVNIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21414..22037
                     /locus_tag="KMT45_000432"
     CDS             21414..22037
                     /locus_tag="KMT45_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003919.1"
                     /translation="MKKPIEEMFLYKLNYYVCLIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRICFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22034..23326
                     /locus_tag="KMT45_000433"
     CDS             22034..23326
                     /locus_tag="KMT45_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE7003920.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23685..23782
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23898..24479
                     /locus_tag="KMT45_000434"
     CDS             23898..24479
                     /locus_tag="KMT45_000434"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBE7003921.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24482..25789
                     /locus_tag="KMT45_000435"
     CDS             24482..25789
                     /locus_tag="KMT45_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBE7003922.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26082..26570
                     /gene="purE"
                     /locus_tag="KMT45_000436"
     CDS             26082..26570
                     /gene="purE"
                     /locus_tag="KMT45_000436"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBE7003923.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILEEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26563..27687
                     /gene="purK"
                     /locus_tag="KMT45_000437"
     CDS             26563..27687
                     /gene="purK"
                     /locus_tag="KMT45_000437"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBE7003924.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27700..28995
                     /gene="purB"
                     /locus_tag="KMT45_000438"
     CDS             27700..28995
                     /gene="purB"
                     /locus_tag="KMT45_000438"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBE7003925.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29175..29552)
                     /locus_tag="KMT45_000439"
     CDS             complement(29175..29552)
                     /locus_tag="KMT45_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003926.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29808..30536
                     /locus_tag="KMT45_000440"
     CDS             29808..30536
                     /locus_tag="KMT45_000440"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBE7003927.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30536..30790
                     /gene="purS"
                     /locus_tag="KMT45_000441"
     CDS             30536..30790
                     /gene="purS"
                     /locus_tag="KMT45_000441"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBE7003928.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30792..31463
                     /gene="purQ"
                     /locus_tag="KMT45_000442"
     CDS             30792..31463
                     /gene="purQ"
                     /locus_tag="KMT45_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBE7003929.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31464..33686
                     /gene="purL"
                     /locus_tag="KMT45_000443"
     CDS             31464..33686
                     /gene="purL"
                     /locus_tag="KMT45_000443"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBE7003930.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33671..35110
                     /locus_tag="KMT45_000444"
     CDS             33671..35110
                     /locus_tag="KMT45_000444"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBE7003931.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35133..36185
                     /locus_tag="KMT45_000445"
     CDS             35133..36185
                     /locus_tag="KMT45_000445"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBE7003932.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36182..36760
                     /gene="purN"
                     /locus_tag="KMT45_000446"
     CDS             36182..36760
                     /gene="purN"
                     /locus_tag="KMT45_000446"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBE7003933.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36757..38292
                     /gene="purH"
                     /locus_tag="KMT45_000447"
     CDS             36757..38292
                     /gene="purH"
                     /locus_tag="KMT45_000447"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBE7003934.1"
                     /translation="MTRALISVSDKNGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFVQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38308..39582
                     /gene="purD"
                     /locus_tag="KMT45_000448"
     CDS             38308..39582
                     /gene="purD"
                     /locus_tag="KMT45_000448"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBE7003935.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39704..40618
                     /locus_tag="KMT45_000449"
     CDS             39704..40618
                     /locus_tag="KMT45_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBE7003936.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40751..42316)
                     /locus_tag="KMT45_000450"
     CDS             complement(40751..42316)
                     /locus_tag="KMT45_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBE7003937.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42309..43760)
                     /locus_tag="KMT45_000451"
     CDS             complement(42309..43760)
                     /locus_tag="KMT45_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBE7003938.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMNTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43774..44790)
                     /locus_tag="KMT45_000452"
     CDS             complement(43774..44790)
                     /locus_tag="KMT45_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE7003939.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRSGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45010..46098
                     /locus_tag="KMT45_000453"
     CDS             45010..46098
                     /locus_tag="KMT45_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBE7003940.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46152..47678
                     /locus_tag="KMT45_000454"
     CDS             46152..47678
                     /locus_tag="KMT45_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBE7003941.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47680..48885
                     /locus_tag="KMT45_000455"
     CDS             47680..48885
                     /locus_tag="KMT45_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7003942.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49142..50119
                     /gene="guaC"
                     /locus_tag="KMT45_000456"
     CDS             49142..50119
                     /gene="guaC"
                     /locus_tag="KMT45_000456"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBE7003943.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50383..>51803
                     /locus_tag="KMT45_000457"
     CDS             50383..>51803
                     /locus_tag="KMT45_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBE7003944.1"
                     /translation="MWEKVEKKKGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLT"
BASE COUNT        16518 a         8826 c        11361 g        15098 t
ORIGIN      
        1 gcttagcaga attaattttc tctttgacaa ctccagggaa tgcattacgt gctgcacatg
       61 aaattaataa atggtttcct gaatataaaa atttcaactt tcttaataag ttagatttag
      121 gtatttcatc atttggaaag ccgttttttt tagacatgaa tatcttattt ttattgctat
      181 ttttcttaat tttccttcta acatatagga aatgtcaaaa ctattatgtg cgtatattga
      241 ctgcattacc gtttttctta aacctgatta tttattttgg taatacaatg ggtcaaagct
      301 ttacttacgt caatggaaat aaaagggcaa tgatttggag tagtagtaac ctgaataatc
      361 tctttacaga gttaggaacg aaactgtcac ttttttatcc aggtacttgg atagctactt
      421 tagtagtctt ggcattatta ctatgtttaa ttgttggaat ttatttaagt tttgacaata
      481 agaaaacttc tattttttta gttattttga tgataatggg attttgttca aggttaatta
      541 tgggattttc tcccacagta tgggcttccg gaatgcggac gtactatata ctttatgttg
      601 ttatcgccat cttagtacta atggcagtta aagagctaat gaaaagcatg agtgtacaaa
      661 aaaatgaatt tatgcaattt ggattaaccg tgcttggtat ttgtaccttt ataataacgg
      721 tgataaacag ataatataca aaaaatccct cgctttagta ttaggtgagg gattttactc
      781 gtttttcgat taattgtttt aacacttgca agtttctacg agtagcttgt ttccgacctt
      841 catatactca cctcaggctg tttacatgat tgtacgttct ttttgtaaag gatacagtga
      901 tatgtatata atggcataaa gaaacgttca aacaaaaaaa ctcgtgaagc aaaattaact
      961 tcacgagttg tagtgcagat gatgggactc gaacccacac gagctaacgc tcacatgacc
     1021 ctcaatcatg cttgtctgcc aattccaaca catctgcgta ataataacac actaatgata
     1081 gcgaggtttt acatagttgt caagctcaaa aatgggagga cttattaaga tggagaaata
     1141 tgacggagaa ttttcaggac tgggaatgat cttaggtgta ttgattggac ttgcattcgg
     1201 acgttttttg cttggtttta tgctaggaat catttgtgga attgcaatgg attgggcagc
     1261 gaatctatgg aatgactatc atgacaatta agttctcaac gcttgtttgt gtttttttaa
     1321 tacttacgtt tttcctaaat aagtggcaaa attttctagg atggagtatg atattgaaat
     1381 agtaaataac aaaaaatttg gaaagttcta actttttgaa atgagggatt ggctaagtga
     1441 tgagagaatt tgaaaaatcc aacaaattag aaggtgtcag ttacgatgtt cgcggtcccg
     1501 ttctagaaga agcggagcgg atgcaagaag agggcattag tattttaaaa ttgaacacgg
     1561 gaaatcccgc accttttgga tttgaagcac caaacgaagt cattcgtgat ttgatcatga
     1621 atgcccgtga ctcagaaggc tactcagatt ccaaggggat cttttctgct agaaaagcaa
     1681 ttgagcaata ttgccaactg aaacattttc cgaatgtgac gatcaatgat atctacaccg
     1741 gcaacggagt gagcgaattg atcacgatgt gtatgcaggg gttgctggac aatggagatg
     1801 aagtactggt tccaatgccg gactatccgt tgtggacggc ttctattgca ttagctggag
     1861 gcacacctgt tcattatatt tgcgatgaag aggcagaatg gtatcctgat attgatgata
     1921 tcaaatcaaa aataacgagt cggacaaaag ctattgttat catcaatcct aataatccta
     1981 ctggggcact ttatccaaaa gaattattag aagaaatcgt tgaagtagct cgacaaaata
     2041 acctgattat ttattccgac gaaatctatg atcgcttagt gatggatggt ctagaacata
     2101 ttccgattgc tacattagcg cctgatttgt tcgttgttac gctgaacggt ctatccaaat
     2161 ctcatcgtgt agcaggattt cgagtaggct ggatggtcct aagtggagat aagtcacacg
     2221 tcaaaggcta tatagaaggg ctgaatatgt tgtcctccat gcgtttgtgt tccaatgtat
     2281 tgtctcagca aattatccaa accgcgctag gcggttatca aagtgtcgat gagctgctcc
     2341 tgccaggcgg aaggatctat gaacaaagag aatatatcta taatgcaatc aatgatattc
     2401 ctggtctttc agccgtaaaa ccaaaagctg ctttttatat attcccaaaa attgatacaa
     2461 agcgattcaa tattttggat gatgaaaaat tcgttttgga ctttttacat gaacatcata
     2521 ttctacttgt acatggtggt ggatttaatt ggaaccagcc agatcatttt cgaatcgttt
     2581 atcttcctaa aatggatgat ttgaaacaaa cagctaagaa aatgagagaa ttcctagcaa
     2641 cctatcgcca aaaatgattt taaaaaccaa atttttcagt aggagggcca cagacaaaca
     2701 cactgaggct ctctttctag tggagttttc ttaaataact aaagtatatt atttagttgc
     2761 aagtcttttc ttgaagtttg cgacttccta aagtaaaata cgaatagaat gcgaatacgc
     2821 atattttggg gaggatactt atggaaacgt taatcgagtc ctttggaacg atcattaaag
     2881 agattcgaaa agaacaaaaa atgacgcaaa aaatgctttc gcaagatatt tgttcacaaa
     2941 gtgtattaag tcggatcgaa aataatgagg aactgccgaa tgtattggtg atgatgcaga
     3001 tttgtcaacg gctaggggta acgattgatc atgtgatgaa tctctctcga aaaactactc
     3061 ggacaaacga taaattattt gaactgttgg atagttgttt ccaaaaacgt gattatcgga
     3121 agttagaaca agtactaaaa tcttctgata tttctgaaaa tctgtatcaa gcaactgact
     3181 ttcaacgcta ttattattat ttcggtgttt gcgaattcac gttgcgtcaa aatatctctc
     3241 agtcccttta ttatttgaaa gaagcattaa gctactctag ccaaaaagaa agaatccatg
     3301 tttctgacac agaaatacaa ctgattagtt gtatcggaaa aatatatggt gtggcaggaa
     3361 aaacggctga agcacgctac tatttgagtc gaagcatcca actgctgcat caatcagctg
     3421 atgaacgatc aaaaagtgaa ttttcgcaaa ttttttataa ttatggaaat ttcttattcc
     3481 atcaaaatga aatcgacgaa gcgttagaac aggtgaatca aggaatctat tgggctcaag
     3541 agaagaatag ctactattac ctcaatgatt tatttgtgct taaaagctta atatacaagc
     3601 gtaaagaaga accagaaaga gcagcattct atgaaggctt agcacaagct gtgaaacaaa
     3661 tttcaaaaaa tttgtaaatg gtgtagacca ttcttgactt cttctctaaa taagagtact
     3721 ttagaggtag aaaaacaaag ggagagaata gaatgagaac ttttatctat gctgacaaat
     3781 tctttttagc gtcaggtgtc aaacatgcag gctatcttga aattaccgat ggactttttg
     3841 gtgaatacac caaagaacta cctgagggag aagtcaacat cattgaccaa agcggcaaat
     3901 ggattgctcc tggtttggta gatacacata ttcacggcta tatgaatcat gatgtaatgg
     3961 ataatgatgc agaaggcatc aaagtgatgt ctgaaggtct gttatcttgt ggagtgactt
     4021 catttttacc aacaacattg acgtcaagca aagaacgttt aaaagatgta gctgagacaa
     4081 tcggcaaaat gtatcaggaa gcaccaggag ctaaaatcaa aggaatctat tttgaaggtc
     4141 ctttcttcac agaagaacac aaaggggcac aaaacccaag ttatttcggg gatcctgatt
     4201 tggatacttt ccatgaatgg caagaagctt caggtggttt aatcaaaaaa attgctttag
     4261 caccggaacg tcaaggtgta gaagagtttg tcaaacaagt gactgaagaa ggagtagttg
     4321 tttctctagg acacagtaat gctacactag aagaagcgca aaaagctgtt gaagcaggcg
     4381 caagtgtatt tgtccatgct ttcaatggta tgcgtggatt gaatcatcgc gaaccaggta
     4441 tggtgggtgc gttattatct ttagaacatg tcttttctga attgatttgt gatggccatc
     4501 atgtacatcc aaatgctgcg ggaatcctaa tggaaaaagc aggacatgat catgtcgcat
     4561 taattacaga ctgtatgatg gccggtggga tgcctgatgg aaattacaat ttaggcgaat
     4621 ttccagtagt agtaaaagaa ggaacagcac gtctagaatc tggcaactta gctggaagta
     4681 tattgaaatt gaaagaagca attaaaaatg ttgtagactg gggattagca acaccagaac
     4741 aagcagtcat gatggctagt ctgattccag caatcagctg taaaatcgat gatacatgtg
     4801 gaaaaatcgc tcaaggacgc gatgcagact ttatcgtatt gaaccctgat atgacattgg
     4861 aagcaactta tctagatggc gtggaacgtt tccacgcata atataattta agttaacctt
     4921 aaaactaaac ttcttaagtt tggttttaag gtttttttgt gttctagtaa aaatcacttt
     4981 acataagttt caaaagtttc tttaatttcc tattgttcaa taagtaacaa tgtaaagata
     5041 aaattaaaaa tgttaatacg tattagcaaa ataaaagcgt ttttttattg tgaataaatg
     5101 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
     5161 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
     5221 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
     5281 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
     5341 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
     5401 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
     5461 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
     5521 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
     5581 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
     5641 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
     5701 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
     5761 cagactgcac cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg
     5821 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
     5881 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
     5941 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
     6001 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
     6061 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     6121 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     6181 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     6241 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     6301 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     6361 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     6421 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     6481 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     6541 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     6601 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     6661 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     6721 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     6781 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     6841 atcaatgaag gatttttagc tcaaatgaaa aaaggcagca tcttgattaa tacagcccgt
     6901 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     6961 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     7021 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     7081 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     7141 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
     7201 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
     7261 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
     7321 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
     7381 cggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
     7441 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
     7501 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
     7561 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
     7621 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
     7681 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
     7741 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
     7801 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgtca
     7861 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
     7921 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
     7981 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
     8041 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
     8101 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
     8161 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
     8221 cccgacaccg tcatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
     8281 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
     8341 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
     8401 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
     8461 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
     8521 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
     8581 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
     8641 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
     8701 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
     8761 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
     8821 aatcgtctct ttaaacaaaa cgtcatatag attatcactg attttttttc ctacttcgaa
     8881 atccaatagc tgctctgttt ggtagaaaat gttttttgca ggtgtattga tcaagaggat
     8941 agttttatca gaagagatgg ctaagattcc ttcatctact tcattttcga taatcgtttt
     9001 ttcacttaac tgttctgcta tttcttgtgg ttcaagtcca agcagtactt ttttcatttt
     9061 ttgtccatct ttgacttgat tgtcaatcgt gttgtttgtt aagccaacac aaactgcacc
     9121 aataatctct tgatcataat aaatagggac gaaatagcgg aagccgaacc ctaacattcc
     9181 ttctttttca gagaaaaatt cttgtcctcc aatcactttt ttcacatctt catgacttga
     9241 gaaaggaaga ccgatgtaac ttgggtctgc gtgagaatat cgaatgcctt cagggtccat
     9301 gacaacgata aaatctaaat tcgatgcaat catgatttca tttgttattc cttgaatcgc
     9361 atgtgtcggg tcaccagtct gcagcttttt ttggatgagt ttatcattgg caacgatttt
     9421 agcaatgcta gagattttat ctgacacact agatcggttc atcgaaagaa taaaattcct
     9481 gatcaatatg gcagaaacaa tcaaagaaac gaatgtggag aaacaaacga caaggatgat
     9541 cgtgttctgg agctttaatg gttttttctt aaaaaaagga aacataggct acctcgaaag
     9601 aatttattgt gaaaatttta acatgaaaac aaaaatttaa caaattgtca acgataaaca
     9661 caaccttcct ataaaaaaca ccactgagtc ggtaaaagaa gctatacaga attttcaaac
     9721 attggtatga tagacgtaga aatggaagga ggcgtgtata tgataggggg aatcgcactt
     9781 gagacgattt attggtatac attgctgatt tgtgcaggat tggcagtcct attgattatt
     9841 tttggagatg tattcgattt tgatggcccg cttgatccga tgcttattat cccttggtta
     9901 actttcacaa gcttatttgg ttttctaggt gagcgtttaa cagatcaatc atctttattt
     9961 ttatttattg ctagtggagg catatcgata gtgttggtat ttttgcttaa tttctatata
    10021 ttgatgccga tgaaacatgc cgaatcaacg atgtcgtctt ctgaaaaaac tctggaagga
    10081 caagtagctg ttgtgatcac accgatccct gtacgaggta tgggagaaat tcagttgaaa
    10141 agtgttaccg gttccattag tcgtccagct tgtttttaca gcccccaaga acagacagct
    10201 gagcggggaa cgaaagtatt gatcattgag atcaaggaaa gaatctgtta tgtcatgcct
    10261 tatgaaagca tgataaaagc ttaaaggagc gtgtgttaaa tggaaatacc agttgttttt
    10321 atcgcgatcg caatcgcagc tttgatttta ctgatgttgc tgatcgtctt tgtagcgaag
    10381 taccagacag caaaaccgga tgaagcgttg atcatcagtg gaagttattt aggaagcaaa
    10441 aatgtgcaca ttgatgaaag caataacaaa atcaaaattg tgcgtggagg tggcgcattc
    10501 gtcttgccag tttttcaacg ttcgaatcgg atcagtctat tatcgagtaa attggacgtc
    10561 tccacccctg aagtgtatac ggaacaaggc gtaccggtga tgtgtgatgg tacttcgatc
    10621 atcaaaatcg gttcatctgt agaagagatt gctacagcag cagaacagtt tttaggcaag
    10681 acaagagaag aactggaaaa tgaagcaaga gaagtattag aaggacactt gcgctcgatt
    10741 cttggttcta tgactgtaga agaaatctat caaaatcgcg ataagttcag tcaaagcgta
    10801 caagaagtcg cttctgtaga ccttgcaaaa atggggttga tcatcgtttc tttcactatc
    10861 aaagaggtca gagacaaaaa cggttatctt gactctttag ggaaaccaag aatcgcccaa
    10921 gtcaaaagag atgcagatat agcagaagcg gaagctctaa aagaaacgcg tatcaaaaaa
    10981 gctcaagcag aaaaagaatc acaagcagct gaattacagc gacaaacaga gatcgcagaa
    11041 tcattaaaag aaaaagaact gaaattagca acctacaaac aagaacaaga tgttgctaaa
    11101 gcaaaagcag accaagccta caatttagaa agtgcccgag cacaacaaca agtaatcgaa
    11161 caggaaatgc agattaaaat cgtagaacgt caaaaacaaa tcgaactgga agaaaaagaa
    11221 atcacacgtc gtgaaaagca atatgattcg gaagtgaaga aaaaagcaga tgccgatcgg
    11281 tacgctaaag aacaagaagc acaagctcaa aaagtcaaag aagtgactga agccgaagca
    11341 gaacgattcc gtgtggaagc actggcagaa gcggaagcca ataagacacg tttagctgga
    11401 caagcagaag ccgaagcagc actagcaaaa ggaaaagcag aagccgaagc aaaacaaaag
    11461 atcgccaatg ccttcaagga atatggcgaa gcagctgtct tgagtatggt tatcgatatg
    11521 ctaccgcaat tgatgcgcga agcagcacag ccgctaggaa atatcgaaaa aatctctgtt
    11581 gtcgatacag gaagcagttc aggagaaacc agtggagcga accgagtgac aaattatgcc
    11641 acaaatttgc tttcttctac tcaagaaaca ctaaaagcga caacaggact tgacttgaag
    11701 gaaatcgtag agaatttgtc gacacgtggg actactagcc aagtaaatat gtatgaagcg
    11761 ccaaaagagg cagaacaaaa ctcgatgaag gaagaataaa gtagtacact tcaccgttca
    11821 atcaaaaatg gaggaagact ttttaaagtt cttctctcca tttttttatt ttattgcaaa
    11881 agaaaattag tcctcgctat ttttcttttg tttttggatc ttagcttcta tctcttgtaa
    11941 atttgttctt agcaatcgtt ctttcttttg gagttcgacc aaatcttctg atagttcttc
    12001 cttcttttct gtttcaggag gacgaagaaa gaaattgata ccgattgcaa tgaatgtccc
    12061 tataaaagta tctagtacac gttggatagc atacaaagcg gattcgcctt gagggatgct
    12121 caacgcaatc agcaacaacg tagcgattgc ggaaatgata ccggaattat tgttaatacc
    12181 atctgacacg acgatcacaa taataactag aataggaagc aagatcaatt ctaccaaaaa
    12241 atcattgtga aaatagcttt tgacaaaaaa ataaatgata gctaatgtac cacctaatgt
    12301 attgccgatg atcctagact ttccaaaaga aacggttgta gtcaagtcct gacgcaaaga
    12361 aaaaacagca gataatgcag caatcatcgg gattcctcgt tgagtcaaat gaaaaaacaa
    12421 gatacataaa aaaacagcta aggcactttt gatcgtacgc atacctagtc gaaaacggcc
    12481 aaattgcata gagattctcc tttttggtct ttttactatc atacctaaac ttttgtctga
    12541 tagctagttt ttcattattc aagtataatt aaaataaaaa agggggaagt aaatgcgtat
    12601 aggaatgcga acagtcaaaa cagtgatcag tgtcttcttt tcgatggtca cagcgggtag
    12661 cttatcgctg ttgtattggc cagctgcagg gatcattgca ttattaagtg taggaaatac
    12721 caagcgctct acattgcgta cgggcatgaa tcgaatcgca gcctttatat tagctacggc
    12781 aacagcactg ttcagttttt ttgctgttgg gtatactatt tttggcttca gtcttttttt
    12841 attcctgttc atcccgcttg cctctcgctt caaattaacc gaagggatag tagtaaattc
    12901 ggttttggtc actcattatt tagtggagga aaacatgtct tggcagctga tcggcaatga
    12961 agcgggatta atggcgattg gattagtatt tgctttgctt gcaaatgttt acatgccaga
    13021 tcgtacgaag cagctgaagg aaaaccaaat acagatcgaa aaagaatttc gaaatatttt
    13081 gagacaaatg gcagaatttc ttttatcaga aaataaagaa gatgtgcaga tcaaatgcga
    13141 acatttactg acattcattc gcgaaagcca agaagatgcc agagaacatc aagaaaacta
    13201 ctggctcaga cagccgctat actacgaaac ttacttctct atgagaagag cacaggccaa
    13261 tgtgattaaa gacatgctgg aaaatctaga gagaatccag caaccagctt attatggaaa
    13321 acatatctat ggtttattga tttatactgc agaaacattt tcagagtcaa atgatggcag
    13381 acaaatttta gcacgtattg aagaagttta tgtattgtat cggcaaatgc ctctgccgac
    13441 tagtcgccct gaattcgagg atcgagcaga attgttccag tttttgcagt cttttaaaag
    13501 ttttattgaa atcaaggcag aattctccca acaaaaaatc caaaaataga ggttgtgaat
    13561 tcagctaata ttcaggtcaa aaagctgatt tcacaacctc ttctttaaac ggtgttctcc
    13621 tgtcaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ttttttgaaa
    13681 ttgctccttg tgactcggag ctgaacggta ggctcacaac ctctttctta aacggtgttc
    13741 aaaagctttc ctctgcggta ataagtccag ccaaacgaaa aataggagga gctatttttg
    13801 tttggctgtt cttattactt gatgcagaac agctttttca caacctttta aaattacata
    13861 tttttccata gaacttgcat ttgatccaag atttcgatga catcttttgt caagaagaga
    13921 gagcgattgg gcaagtcacc ttcaatcatg tgtaccatat tctttatttc gtaattcatt
    13981 gcttcagata tattcccgct ttcgatgaat tcctttgtac catctgtaaa taagatctcc
    14041 gcacgatcag cacgcgggta atcagcaatc gtcacataac cgttctcaaa ggcaacgata
    14101 ccgacttttg gcatttttgc ctgaaaagtc aagctgacag cagcaagttc attttctttg
    14161 ttacgcaaga tagtgacgga ttgttcgtcc acgcctgttt caaaaggaac cattgtagag
    14221 gcaactactt caggttgaga gctcaaaaag aaacgagcga aagatacagc atacgtaccg
    14281 atatccaaca gagctcctcc agctaattct ggattgaaga accggttttt agggtctggt
    14341 tctttgtaac tgccgaaagg tgcttggatc atttttaaag cccccagttt tcctgtatcc
    14401 attattgagc gtaactgctg ataaagtggc atgttgaaga ttgtcatcgc ttctgctaag
    14461 atcaaattct tttcttctgc taagcgcata gcgtcagcca gttctttttt attcatcgtg
    14521 atggcttttt cacatagaac gtgtttgcct gcttccaatg ctgccaagat atggtcgata
    14581 tgctgtctat ttggtacagc aatgtacacg atgtcgattt cagcatcgtc taacatttct
    14641 tgatagcttc cgtaagcttt tggaatattg taacggtgag cgaagttttc cgctttttct
    14701 gaagttctgg aagcaactgc tgttagttcg ctcgtctctt gattgaagct ttcggcaaac
    14761 tcatgtgcga tggtaccaag accaacgatt ccccattgat attttttcat ctgaaaacgc
    14821 tccttcatag ttggattgag tttcttgtct gtctattatt ataacaaaaa tcacgaagaa
    14881 taaagaggag aggacttgaa acattaaaaa aaatgaaagc agcgatttaa gaggtgcttt
    14941 ttgatcaaaa cttaagtaga atagttaaaa ggaggggata aacgtgcatc aagtatatat
    15001 tgatcttatt atgagtgtat tattagaaca gtttgaaact gaaaaaaatt ttttagatga
    15061 atacttgctg atcagtccag aacaatggca aaaatggaaa gatggcgaga aaaatctgcc
    15121 atctgaagcg atgcagaaag taaaaaatct tttcactgat tatgaatgga tgctaacgca
    15181 aaagattttg cgtcaaacga ttatttttcc cgaaaaacga aacgttgccg ttgcagagta
    15241 caagcaaatc aaaacacgca tcgcgcaaaa atggcttagc agcaaagcag caaaagtcga
    15301 gctgatccct ttgaaaaata atcagcaggc tggtagatac cttgacttga aagtaagtat
    15361 tcaatatgat gaatggggct atgatgatat tttgaatttt cgtctgccag cagatgttca
    15421 aaaacagatc gaaggagaaa aaatcgaact tctcgattgg gtgaatgaaa atctggaaga
    15481 aacttacgtt catgaggcga atggggaacg tgaagaacaa cgaaaggatt tttcaagtga
    15541 ataaattttt aggatttgga gcagttgtga ttatgatcgc agcaatgggc tatgttattt
    15601 ttagcgatga tgattcgcat gttcaagccg gcaatcaagc agtcgaacga acaagcacca
    15661 aaacaactgc tagtaaagaa aagaacagtc aaacaaccag cagcaaggta aaggacaaag
    15721 aaacaaaaga tcgtacgtcc aaacaattgc cagatgttaa aacagacgat tggaagctag
    15781 ttttggttgg tccagatcac aaattagaaa aagaaatcga tgagtctact caattggcat
    15841 cattaagtaa cggatacttg atcgacaaac gcattgctcc agaatatgaa gcatttgcca
    15901 aggctgctga agcagctgga tttccattag tgatggtctc agcttaccgg tctgtatcat
    15961 cccaacaaca agttttcgaa caaaatgtcc aagatgtgat gagtcgtcaa ggtgtatcag
    16021 aagaggaggc gacaaagatt actaaagaaa cgattacgga gccaggatac agcgaacacc
    16081 acacaggtct ggcagtcgat gtcgtcgatc aaaactggta caacagttat ccttctactg
    16141 tattggatgc aagttacgga gatcagcctg gagctaaatg gatcgcagat aatgctgcgc
    16201 agtatggatt tatcgttcgt tatccaaaag gacacgaaga tataactaag atcacatatg
    16261 aaccttggca cttacgttat gtcggtaaag aaaacgctga gtatatcacc aaacaccaat
    16321 tgacgctaga agaatatctg aatgaattaa atgaaaagta ggaattgatc atggcgaaaa
    16381 aaaagtggaa aaagcaaaag cgcaagaata tccgttggcc agcagtggtt gccggtgtag
    16441 cagtgatact cttagccttt gttttttcct tgaagaatct gtcttctcct tttactgata
    16501 atcagcaaga aaatgaagcg caatcccatc agcaatttat tgaacggctt gttccgcatg
    16561 cccaagaact acaagatggt tatggagtgc ttcctagtat cattttagga caagctatat
    16621 tagaatcaaa ttggggaaaa agtaccttgg ctagcaaata caataatctt ttcgggatca
    16681 aggcttatgg ggatcagaag aaagtaagcc tggaaacaaa ggaatttgtt aacgaagaat
    16741 ggataactat ccaaggagac ttcaaagtgt atgattcatg ggaacagtcc atggatgacc
    16801 atacacaact gttcgtccaa ggagtagatt ggaatcctgc gctgtatgaa aaagtgatta
    16861 cggccaccaa ctatcaggaa gccgcacaag cattgcaaga tgcaggttat gcgacagacc
    16921 ccggttatgc gcaaaaaatc atccaggtca tcgagacgta tcaattgaat cagtatgacc
    16981 ggagatagca aaggagaagt ggctgatggc agaacaattt acgccagaaa tcatgacgga
    17041 attgagaaaa gatatcgtaa gagtaccgga aatcatttta gaagcaagcg ggatcaagat
    17101 atttggaaaa ttgatccgtt cgatcatctt tactacggat attgcgatca tacgtaatac
    17161 aaatgcgaat gcagtaattg ctgtgtatcc ttttacacct catccagcga ttactaaaag
    17221 cattattgag gcagctgaca ttccagtttt ctccggtgta ggtggtggtt tgacacaagg
    17281 tgctcgttct tcctacatga gcatgttcgc agaagcgcaa ggttcgttag gggtcgtttt
    17341 gaacggtcct acgcctttag agacagtaaa agaagtttgt cgagtagttg atattccagt
    17401 tatcagtacg gtaacgtcga aatatacaga aatagatgaa aagttagcag ctggtatttc
    17461 gattatcaat atcagtgcag gtaaagaaac tgcagaagca gtacgccatt ttcgtaaaaa
    17521 atatcctgaa cttcctgtca ttgcgacagg tggtccaacg gaagaaagta ttgctgagac
    17581 cattcaagca ggagcaaatg ccatcacata tacgccacca agtagcggag agctttttag
    17641 tcgtaaaatg gaaaaatatc gggaaactga gaaaaagaat catgaataac aggtgagaat
    17701 caaaagacga ggaagagcag atagtggctc ttcctcgtct tttgtactcc agtactattt
    17761 gttcagaatt caaaaaaaag agaaattttt ttgttttttt atctgtaatg tacataagaa
    17821 gagttaaaac cttttgttat tattagcatt ctaacaaaaa aatgtcttga tttattgaag
    17881 cgctttctat agaatgaaaa acgaataaaa actaaaaaag gcacccgaca atttagtagc
    17941 agctaaattg tcggggcttg aaaagtcagc gggaactgac caatcaagtt gccatagtca
    18001 atgcttgtgc atcgacgtgt atattgtacc taatttcata cagaattact agttattttt
    18061 ctgtttttta gggaagagcg cacacgaaga agcatgctta tttagctatg tcaaggatat
    18121 tgggcagttg tctgatatcc ttttttcttt gcgtaattat cctcccatca ttgaagcaag
    18181 aagtgttttt agcgatgaaa gaagaatcag cactatctaa ttttccttga aagtattgag
    18241 ttgttcatcc taggcagaac gataaggcaa gtcttggttt ttattcccct tgaaaaagat
    18301 cgtttaataa atatgttctg tataaaaata cagtatattg aaaaataaca atggaagtag
    18361 aggtgcagag aatgaagaaa agtatgagaa tcatcttagc tttaagtgtt atgttattct
    18421 tcttgacggc ttgtgggcaa gaagatgaca aaaaaatcac gctgaacatg gcttggtggg
    18481 gttcgcaagt ccgacacgat gcaacagtaa aagtaatcga gctttatgaa gaaaaaaatc
    18541 cccatgtgac gatcgattat gaattttatg attatgaagg atattacaca aaattaggta
    18601 cactagcagc atcagatgat atctgggaca ttttccaact gaacaatcaa tttcctcaat
    18661 acatcaatca gattgaacca ttagatgaat atgtggagca agggataatt gatatttcta
    18721 atgcagatga atcctattta gcaacgacga cttacgacgg gcaattagtt ggactgtcta
    18781 acggggtgaa ttcgtttgcg attgcttaca ataaaaatat atttgaacaa ttaaatatcc
    18841 ctgaaccagc ttcaaattgg acatgggatg aatttgaaca aatcagctat caaatcactg
    18901 aagaattagg aatctatgct atttcaagat tcgaagattt tatagctggc tgtattattc
    18961 aaataccaca agttgagaaa ggattgaatt tttataatcg ggaagatcta tctaacagct
    19021 tgggatttga aaatccagac tatctaacag attttttcca gctgcgtaaa aatctggtag
    19081 acaaaggggc ccatccagaa ccaggtggtg gagctgcaag tacgacggat ctgtcttttc
    19141 aggcggttag ggatagagaa gcggcaatgg tgtttctatc aagtaaccag ttgacagaaa
    19201 tattgtcagg ggctcctgaa gatatggaga ttcagttgat taatccacct cgcagaaaag
    19261 cagatggaga atctggtatc cctttacgtt cttcgcaaat gctgagtatg gcaaaaagct
    19321 cagcccataa agaagaagct gctaagttta ttgatttctt tcaaaacagt gaagaagcca
    19381 atgaaatttt aaaaggagaa cgtggtgtac caatcatgtc gaatattcga gaacagctcg
    19441 ctcgtgcaga tgatgcaact ttgcaagcga catttgatta tttagaaatg atttccgata
    19501 tggacaacgg cgaagtgaat gtctttgata gtgctttgaa tccggaaatt caagaccaat
    19561 acaatctttt agttgaaaaa gtcatttatg aagaagtaac agcagaagaa gcagcagaga
    19621 gtctttatga gtttgcgtca aacattctag gatagcagca ggaggaaggc atgaaaaaag
    19681 aaaaactaaa acggttcttg gatcaagaca atgtggtcgg ttacgtattt gcttctccat
    19741 tcatcctagg atttctattg ataacagtga taccgatgat cttgtcactt tattattcgt
    19801 tcaccgatta tcgtttggga aatccagtct catggatcgg cttggataat tatataaaga
    19861 tgctgagtga tgcccgtttt ttgaactcca tccgagtcac ttttctctat gtatttgcat
    19921 ctgtacctgt gaaactgatc tttggtctat tgattgctta tcttttaacg aggaatatca
    19981 aaggaacgac tttctttcgt tctttgtatt atgtgcctag tttgatcggt ggcagtattt
    20041 ctatcgctct agtttggcgg gagttgtttt ctaaaaacgg gttagtgaat attttcctgg
    20101 aacgattagg cgcacctgcg ctttcctggt ttagcgatca aaaactggtc atgatccctc
    20161 ttgttttgat gtctgcttgg caattcggtt cggcaatgat catctttgct gcaggtatca
    20221 aagagatccc aggctcgtat tatgaagcgg cagagatcga tggagcgaac aaatggcaga
    20281 cgttttacaa gatcacgtta cctcttcttt cgccgatcat tttgtataac ttggttatgc
    20341 agacaatcgc ggcgtttatg acttttacgc aggcatttat cattacccaa ggtggtccga
    20401 atgatgccac gaacttttat gctctttatg tgtataacca agctttttct tttaattcta
    20461 tggggtatgc tagtgctatt tcgtggatca tgctgttgat tatgggagct gtcacctttc
    20521 tgattttcaa atcatcaaaa aaatgggtcg ttgtgtgaat gagaggagga agaaatgaat
    20581 aatacagtaa cgaaatggtt tcaatacggt tttattttgc tattaggtct agtcatgatt
    20641 tatccggtct tatggatgat cgcaggttct tttaaaacgg atcaagagat tatgagtggt
    20701 tcgctgaatt tgattccaga gacttttcaa tgggaaaatt acgcaaaagg atgggcgggg
    20761 tttgctggaa tttcattttt tactttcttt aggaattcgt ttgtcatctc tgcagtttcg
    20821 acgataggga cagttctgag ctcgacttgt attgcttacg cattatcgag aatcgatttc
    20881 aaaggaaaaa gattctggtt cgtcgtcatg ttagtaacga tgatgattcc tgcacaagtg
    20941 attttgattc cgcagtttat tatttacaat cggctgaatt tggttgggac gtatgtgccg
    21001 ctgattttac cacatttttt tggacaagca tttttcattt accagatcat gcaattcatg
    21061 gtcaacatcc ccaaagagtt agatgaatca gcgattattg acggatgcag caagtatagt
    21121 gtctttacca aaattatttt tcctttgctg aaaccttcga tcatcacgac gatcatcatc
    21181 cagttttatt ggaaatggga cgacttcatg ggaccattga tttatttaaa caagccgaga
    21241 tcttacacgg tatcgattgc catcaaatta tttgcagacg ctggatctag tacgacttac
    21301 gcgagtatgt ttgcgatgtc tacgttgtcg ctgctaccgg tttttctgat tttcttattc
    21361 ttcaataaat atcttgtcca ggggatcagt acaagcggtc tgaagggctg aatatgaaaa
    21421 agccaataga agagatgttt ctttacaagc tcaactacta tgtctgtctg atttttttaa
    21481 ccagtcttat cagtaatgct cttttcgctt tagttctagg atgtttgttc actttaggat
    21541 tagtgctgtc tgctcgtgtt gtctatctga tttatttttt gatgatgtat ccttcttatt
    21601 atctatctgt ccattacttg gcagaaaaaa aggtatgttg gaggaaagga aaaatcaaac
    21661 ggatatgttt catcattacg ttgtttcagc tgtttgtcat tatttttgca gttgattggt
    21721 atttattagt gggaaagatt ggagcttggt atctgattcc ttttctattg tgtctgttgc
    21781 tttttgtatc ccatagtttt tttagtacgc taattgatat ctgcaaagaa gataaagcag
    21841 atagtcttgt ttatttgatc aaaaggaata tctggctgtt gtcaaaaaac gtcaaggggt
    21901 ttctgtggta tttatgcctt ttgtatggac tgttctggtg tatacagcat gattttttac
    21961 tcactgcatt actgtatcca gggctgccat ttgtcacagt attttgctta aaagaaaagg
    22021 agaatgactt taaatgaaga aaaaatatgt acaagtcgga cttggtggga gagcgcgttt
    22081 tttttatgag gcaattgcct ctacgtatca tcaaacttgt gaactagtcg gtttttgtga
    22141 tgtatctcaa acaaggatgg attatgcaaa tcggatactt gtgaacaagt atggacagaa
    22201 aaaagtaaag acatgtatgc catcacaatt tgagcaacta ctggatgacg tacggccggc
    22261 tgaagtcatt attacgtcag tagatcgaac gcatcatgat tatatcattc gtgcaatgaa
    22321 aaaaggatat gatgttattt gcgaaaaacc tctgactatc gatgaggaaa aagctcaaga
    22381 aataataacg gtccaaaaac agacgggaag aagcctgcga gtcactttta attatcgtta
    22441 tgcaccgtat cacacgaaga taaaggaact gatagaaagt gatgtgattg gtgatgtgtt
    22501 ttctatccac ttcgaatggc tgttaaatac gcaacacggg gcagattatt tcagaagatg
    22561 gcatcgaaat aagcacaata gcggcgggct tcttgttcat aaatctaccc atcattttga
    22621 tctagtgaat ttttggttga acagccgtcc ccaaaaggta tttgcattcg gtggattaaa
    22681 cttttatggg atgcataatg cggagaaacg cggagtagaa atattttaca gtcgatgcta
    22741 tggaagcgaa ctagctaaag ctgatccatt tgctatagac ctgtcagcca atgaggaatt
    22801 gcgagaaatg tatttagaag cagaaaaaga cagtggatat attcgtgacc gcagtgtatt
    22861 ttccgatgat atctctattg aagatacact aggcgtaacg gtcaaatatg aaactggtgc
    22921 gattttgacg tattcgctaa acgcgtatat gccgtgggaa ggattcaatg tttctttcaa
    22981 tggcagcaaa ggacggatcg aaatgaaagt gatcgaaaag tcttacatca atgcaggtgg
    23041 agataaaaag gatgaaggag cagccgttct tcagtccatc catgtctatc cgatgttcgg
    23101 tgagccatat gaggtaatcg tcgaacaaga agcgggcgga catggcggag gagatccgcg
    23161 tttattagat gatctatttg gtgaagaaaa agaagaagat ccgtggaatc gggcagcaac
    23221 tcacgtagat ggtattttgt cgattttgac aggaatcgca gcaaatcatt ctattgcttc
    23281 aggaaaagca gtagtgattg acgagcttgt cagcttaaaa gaataagaat agatttacgg
    23341 aaattacttc ccgtagttta taaaatcttg ccttattgcc gataggctgt cttttgaaca
    23401 ctatttattc ggaaaaaggg gctatgacat gctttttgtc atgccttctt tttatgtggg
    23461 ctgctttttt agataggaga aaataaaaat aactatttaa acccgaacaa aaaggaataa
    23521 tgcattaaaa atcattagat tttacaaaaa aataagtaaa ccacgttgtc agtttcagaa
    23581 agtagtgtta gactgttttc aatatttgaa acaacatttt ttattcaaat atggcaaact
    23641 gccttcacgt gcaaaacagt tgcaagaaaa tgaatttaca ctattattag ttttacatac
    23701 ctacttatat atcgtcataa tatggatgac agtttctagc cagtaccgta aatgctggac
    23761 tataagtaaa agattggcta tttcaccctt cttttcttat gataaagtgg agggtgtttt
    23821 tctttaacag aaacatagta ggtttttgca caaaagcggt tatttcggta ttaataaatg
    23881 ataaaggagc aaataaagtg aaagaattag ttgaacgtat tcaaaaagac ggacgtatat
    23941 taggcgaggg cgtattaaaa gtcgacagct tcgtcactca ccaagtagac cctgtattga
    24001 tggaacaaat gggaaaacgg tttgcagaag tatttaaaga acaaggaatc acgaaagttg
    24061 tgaccattga agcttctggg atcgcaccag ctttatttgc tgcaagagaa ttagatgttc
    24121 caatgatctt cgctagaaaa gcaaaaagtt tgacgatgga tgaagaatta ttgacagctt
    24181 ctgtttattc ttttacgaaa caagtgacaa gtacgatttc tatttctcgt aaatttttat
    24241 cttcagaaga caaagtattg attatcgacg atttcttggc aaacggacaa gcggcaaaag
    24301 gattgatcga attatgccag caagctggag cgcaagtaga aggaatcggg atcgtcatcg
    24361 aaaagtcttt ccaagatgga cgtcagttac ttgaagaaat gggattgaga gtcgtttctc
    24421 ttgcgcgtat tgcttccttg acgaatggac aagttgagtt tcttgaggag gacgcataat
    24481 cgtggaaaaa gaaacacaaa atggaaaagc tgcggtttta gggctgcagc atctgctagc
    24541 gatgtatgca ggagcagtag cggtgcctct tttgatcggt acaggactag gctttaatca
    24601 agaacagatg acttatttga tttcaattga tatctttatg tgtggattag cgacattatt
    24661 gcaattgaca gttaaccgat ttttcggaat tggcttgcct gtcgttttag gatgtgcgat
    24721 ccaagcagta gctccactga tcttgatcgg aaccaatgaa ggagtaggag ctatctacgg
    24781 atctattatc gcttctggga tctttgttgt gttagtttct ggtgtctttt ctaaaattaa
    24841 aaaactattc cctcctatag taacaggaac ggttatcacc gttattggtt tgactttgat
    24901 tcctgtagca atcgagaaaa tgggtggagg taatgcaaca gcagctgatt ttggtgacaa
    24961 aacaaatctg ctacttgcat ttgtgacgat tctcctaatt gtaggcgtgc aaatgctggc
    25021 aaaagggttc attcgttcaa tcgcggtatt gattggttta gtcggaggaa gcatcttagc
    25081 tgctttcatg ggaatgattg atctgagcgc gattggagat gcgccgttgt tccatgttcc
    25141 acaacccttt tattttggta aaccaacttt cgacgtatgg tcgattttac taatgatcat
    25201 catttccatg gtcagcatgg tagaatcaac aggtgtatat tttgcacttg gtgacattac
    25261 tgggaaaaat gttggagaag aagaattgaa aaagggatat cgtgcagaag gacttgccgt
    25321 tatcttaggc ggtattttca atacattccc ttatactggc ttttctcaaa atgtcggatt
    25381 ggttcaactt tcgggaatca aaacaagaaa acccatctat ttctctgcat tcttcttgat
    25441 tgttttagga ttattcccta aaatcggtgc gttagcacaa atcatccctg agccggtact
    25501 tggtggagga atgttagtca tgttcgggat ggtggcagtc caaggaatcc gtatgctgtt
    25561 ggaagttgat ttcagcaatg ataaaaatct attgattgtt gctgtttctg ttggatttgg
    25621 gctgggtttc aatgttacgc cgacattatt cagtcaaatg ccgcaaacat tgcaaatgtt
    25681 cactggaaat ggcatcgtaa tgagtagcat tacggctatc atattgaatt tgatctttaa
    25741 tggattgaaa aaagatgaag aaatattgga agaagccttc aagaagtaat agagcaaaag
    25801 aaaggttgtg aaatcagcgt tttgacctga ttgttagaga aacaaacgga aaaatagttc
    25861 ctcgtatttt gccgtttgtt tgagctaata ccgagggtca gctgattgaa caccgtttat
    25921 ccataaaaag gaatgtgacc taacttttgt cacattcctt tttttcatct ttaataaaca
    25981 cgaacaataa aatgtttttc tttgttaaca ttcgaaattt ctttgacatc aaaaaaagtg
    26041 attgttaaaa tatgaatgta gaaaaactgg gaggaaaaac aatgacagca aaaatctctg
    26101 tgattatggg gagtacttca gattgggaaa caatgaaaga agcatgtcaa atattggaag
    26161 aattcggtgt gtcttatgaa aaaaaggtcg tttctgctca tcgaacccca gatttgatgt
    26221 ttgaatatgc acaaaacgct cgaaaaaaag gaatcaaaat cattattgca ggagcagggg
    26281 gagccgcgca tctgccgggt atggtcgcat caaaaacaac tttgccggtt atcggtgtac
    26341 cggtccaatc acgaacatta aatggtttgg attcgcttct ttcgattgtc caaatgcctg
    26401 gaggagtacc tgtagctact acagcaatcg gaaaagcggg agcaattaat gctggactac
    26461 ttgcgataca gatgctttcc atgtatgatt tagaattgga ggctaaattg gctgaaagac
    26521 gttcaatgtt ggctaaaact gttatagaaa gtagtgatca acttggttaa acctttgttg
    26581 cctggttcaa cgatcgggat cgtcggtggc ggtcaattag gtcgtatgat ggcgattagt
    26641 gcaaaagaaa tgggctttaa agtaggtgtg ctggatcctg tgagcgattg tccagccgca
    26701 caagttgcag attggcacat tgaaagtgtc tatgatgaca cctttgcgct gaaagagctt
    26761 gccagaagaa cagatgtcat tacctatgaa ttcgaaaacg tcagtgtaga ggcgttaaat
    26821 gcgattttac ccatgtcatt tattcctcaa gggacagact tgcttgcgat tacgcaagac
    26881 cgcctgttag aaaaatcttt tttggaaacg aacaacatcg tgattgctcc ttatgcaacg
    26941 atcgtcagtc cgacggatat ccaagatgcg attgacggga tcggctatcc ttgtgttttg
    27001 aaaaccactc gaggcggcta tgatggcaag gggcagtatg ttttgaaaag tcgggcggat
    27061 ctagctcctg ccatggacct tttaagagaa ggaacctgtg ttttagaagc atggatcccg
    27121 tttgaaaaag agatttcgat catggtggca ggaaatggtc aaggagattt cacgactttt
    27181 ccggtcgtgg aaaacatcca ccacaacaat attcttcatg aaacgattgc tccagccgcg
    27241 attgatcagg atgtgatcga ggaggctgag cgtattgccc gtgtgattgc agaagcagtg
    27301 agcctttcag gtgttttagg ggtagaaatg tttttaacaa aaacaggcgg gctatatgtg
    27361 aatgaattag ctcctcgtcc tcacaattct ggacattatt ctattgaagc ttgttctatg
    27421 agtcagttcg atgcccatat tcgaggtatt tgtggttggc cattaggaga aaaagtacgt
    27481 ttgataagca atgcagtaat ggtcaatgtt ttgggagaac aactgctgga aagctatcgc
    27541 ttgattgaga ataagccaga ctggcaattc catttctatg gaaaaaaaca agcaaaaaaa
    27601 ggtcgcaaga tgggacatat cactatcttg acagaagaca tccagcaaac gctggaagaa
    27661 atcaaacaaa cgaatatttg ggattagagg agaaaaaaca tgttagaacg ttatacacgc
    27721 cctgaaatgg ggaatatctg gacggaagag aacaaatacc gtgcgtggct cgaagtcgag
    27781 attttagcag atgaagcttg ggcagaactt ggtgaaatcc cgcaagaaga tgtgaaaaaa
    27841 attcgtgagc atgcatcatt tgatatcgat cggatcttgg aaatcgaaaa attaacacgc
    27901 catgatgtcg ttgcgtttac acgagcggtt tctgaaacat tgggagaaga aagaaaatgg
    27961 gtccattatg gtctaacaag tacggatgtc gtagatactg cttatggcta tctcatcaag
    28021 caagcaaatg atattttacg ggaagacttg aaacgtttta cggaaatcat tggcgaaaaa
    28081 gcaaaagaac acaaatatac agtgatgatg ggtcggacac acggtgtcca tgctgaacca
    28141 actacttttg gattgaaact tgccacatgg tattcagaaa tgaaacggaa catcgaacga
    28201 tttgaacatg cggcaaaagg agtagaagca ggaaaaatct caggagcagt ggggacgttc
    28261 gcaaatatcc cgccatttat tgaagagtat gtatgtaaac aattggggat ccgtccacaa
    28321 gaaatctcta ctcaagttct ccctcgggat ctacatgctg aatatttctc agcgatggcg
    28381 cttattgcta caagtattga acgttttgca acagagatac gcggattgca aaaatccgaa
    28441 acacgtgaag tagaagaatt ttttgcgaaa ggtcaaaaag gttcttctgc tatgccgcac
    28501 aaaaggaatc cgatcggttc agaaaatatg gctggtctag ccagagtggt gcgcggacac
    28561 atggtcactg cctatgaaaa tgtcacatta tggcatgaaa gagatatttc tcattcttca
    28621 gcagaacgaa tcatcttgcc tgataccacg attttattga attacatgtt aaatcgtttt
    28681 ggcaatatcg ttaaaaatct aacggtcttt cctgaaaaca tgaagcggaa tatgtctgca
    28741 acatttggtt tgatttatag ccagcgcgtt atgctgaaat tgatcgataa gggaatgaca
    28801 agagaagaag cttatgatct tgttcagcca aaaactgcgt acgcttggga caaccaagtg
    28861 cagttccgcc cgctgttaga agaagatcca accatcacat cggtattatc taaagaagaa
    28921 atagacgatg cctttgatta tcatcatcat ttgaaaaacg ttgacgtgat attcaaacga
    28981 gtaggtttgg aataaagaag agtgagttgc aaaattcaat taaaatagaa tagcaaaaat
    29041 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
    29101 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
    29161 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
    29221 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
    29281 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
    29341 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
    29401 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
    29461 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
    29521 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
    29581 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
    29641 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
    29701 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aacacgaaca
    29761 aaaaacgata tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
    29821 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
    29881 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
    29941 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
    30001 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
    30061 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
    30121 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
    30181 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaaac
    30241 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
    30301 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
    30361 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
    30421 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
    30481 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
    30541 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
    30601 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
    30661 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
    30721 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
    30781 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
    30841 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
    30901 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
    30961 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
    31021 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
    31081 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
    31141 tgaaagtcaa tcccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
    31201 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
    31261 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
    31321 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
    31381 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
    31441 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
    31501 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
    31561 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
    31621 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
    31681 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
    31741 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
    31801 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
    31861 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
    31921 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
    31981 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
    32041 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
    32101 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
    32161 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
    32221 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
    32281 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
    32341 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
    32401 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
    32461 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
    32521 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
    32581 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
    32641 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
    32701 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
    32761 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
    32821 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
    32881 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
    32941 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
    33001 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
    33061 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
    33121 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
    33181 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
    33241 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
    33301 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
    33361 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
    33421 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
    33481 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
    33541 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
    33601 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
    33661 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
    33721 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
    33781 aggacaagaa ggtgcgggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
    33841 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
    33901 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
    33961 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
    34021 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
    34081 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
    34141 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
    34201 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
    34261 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
    34321 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
    34381 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
    34441 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
    34501 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
    34561 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
    34621 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
    34681 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
    34741 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
    34801 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
    34861 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
    34921 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
    34981 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
    35041 tcctacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
    35101 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
    35161 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
    35221 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
    35281 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
    35341 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
    35401 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
    35461 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
    35521 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
    35581 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
    35641 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
    35701 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
    35761 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
    35821 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
    35881 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
    35941 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
    36001 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
    36061 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
    36121 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
    36181 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
    36241 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
    36301 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
    36361 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
    36421 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
    36481 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
    36541 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
    36601 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
    36661 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
    36721 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
    36781 tcagataaaa atgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
    36841 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
    36901 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
    36961 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
    37021 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
    37081 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
    37141 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
    37201 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
    37261 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
    37321 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
    37381 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
    37441 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
    37501 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
    37561 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
    37621 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
    37681 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
    37741 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
    37801 ctgacagtag actttgtgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
    37861 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
    37921 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
    37981 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
    38041 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
    38101 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
    38161 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
    38221 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
    38281 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
    38341 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
    38401 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
    38461 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
    38521 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
    38581 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
    38641 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
    38701 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
    38761 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
    38821 aaccagcatc gatttgggga tagcagcaaa aaaatcgtcg tagaagagtt tttggcagga
    38881 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
    38941 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
    39001 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    39061 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    39121 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    39181 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    39241 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    39301 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    39361 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    39421 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    39481 aaaaaaatct acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    39541 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    39601 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    39661 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    39721 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    39781 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    39841 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    39901 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    39961 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    40021 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    40081 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    40141 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    40201 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    40261 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    40321 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    40381 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    40441 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    40501 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    40561 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    40621 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    40681 tttcagcttc gagatattct gtctttttta attgcaaatg gaaagagtat cattgtacgg
    40741 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    40801 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    40861 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    40921 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    40981 tgaaatgaga gggacttaat gcaacttgtt gagcaaccca agttaaggac atatctggat
    41041 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    41101 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    41161 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    41221 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    41281 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    41341 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    41401 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    41461 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    41521 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    41581 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    41641 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    41701 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    41761 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    41821 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    41881 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    41941 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    42001 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    42061 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    42121 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    42181 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    42241 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    42301 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    42361 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    42421 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    42481 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    42541 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgcggt aatacgaata
    42601 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    42661 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    42721 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    42781 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    42841 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    42901 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    42961 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    43021 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    43081 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    43141 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    43201 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    43261 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    43321 acagaatcta cctgattgat gatctcttca ttggtattca taaaatcttt tgccaagata
    43381 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    43441 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    43501 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    43561 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    43621 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    43681 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    43741 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    43801 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    43861 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    43921 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    43981 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    44041 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    44101 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    44161 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    44221 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    44281 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    44341 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    44401 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    44461 tgatcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    44521 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    44581 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    44641 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    44701 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    44761 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    44821 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    44881 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    44941 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    45001 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    45061 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    45121 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    45181 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    45241 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    45301 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    45361 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    45421 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    45481 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    45541 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    45601 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    45661 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    45721 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    45781 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    45841 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    45901 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    45961 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    46021 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    46081 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    46141 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    46201 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    46261 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    46321 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    46381 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    46441 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    46501 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    46561 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    46621 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    46681 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    46741 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    46801 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    46861 gcgggatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    46921 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    46981 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    47041 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    47101 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    47161 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    47221 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    47281 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    47341 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    47401 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    47461 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    47521 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    47581 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    47641 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    47701 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    47761 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    47821 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    47881 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    47941 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    48001 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    48061 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    48121 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    48181 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    48241 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    48301 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    48361 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    48421 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    48481 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    48541 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    48601 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    48661 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    48721 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    48781 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    48841 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    48901 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    48961 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    49021 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    49081 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    49141 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    49201 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    49261 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    49321 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    49381 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    49441 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    49501 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    49561 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    49621 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    49681 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    49741 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    49801 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    49861 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    49921 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    49981 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    50041 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    50101 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    50161 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    50221 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    50281 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    50341 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    50401 aaaaaaaaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    50461 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    50521 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    50581 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    50641 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    50701 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    50761 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    50821 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    50881 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    50941 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    51001 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    51061 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    51121 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    51181 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    51241 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    51301 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    51361 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    51421 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    51481 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    51541 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    51601 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    51661 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    51721 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    51781 attctagaaa agtggttaac aga
//
LOCUS       DAEGIQ010000009        48703 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000009 DAEGIQ010000000
VERSION     DAEGIQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.009"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(33..1481)
                     /locus_tag="KMT45_000458"
     CDS             complement(33..1481)
                     /locus_tag="KMT45_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013189598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HBE7003945.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPDEYVLAINSMTKPNPTFKKEMKSIKNARGEDDILFII
                     DVEPSISHVIFNSNGDCFLRIGDKSVHQTHDQISKLEYDKGQRMFEDIEILDSTLDDI
                     DESLMDSYKKLKKAEAISTETILTGRGLMRNGHLTNAGMLLFGKNTFLALPNSRIRFL
                     RYDGKRNETGRRLNIVKEFDYEGAIPKLIESITRDVKTQLREFQYLNDDGKFSIIPEY
                     PEFAWFEGIVNALTHRDYSYKGDYVRINMYDDRLEIFSPGALPNIVTLQNMRRTRYAR
                     NPRIARTLSEFGWVKELNEGVNRIYDEMQLLFLNEPKYSEPNNNSVLLVLENSITSRH
                     LRERDRLDDYLGEFEVDSLSTSEKMIVSHLFNNEKISVKEASEVLKKGVGYSRKVLKE
                     MLRKKIIQWHGTSTKDPTQYYSLNRVKQSKIE"
     gene            complement(1627..3123)
                     /locus_tag="KMT45_000459"
     CDS             complement(1627..3123)
                     /locus_tag="KMT45_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBE7003946.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKSFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(3295..3540)
                     /locus_tag="KMT45_000460"
     CDS             complement(3295..3540)
                     /locus_tag="KMT45_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7003947.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRIRLEKGIGQTELIRDLQLHKIA
                     ITRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            3645..4418
                     /locus_tag="KMT45_000461"
     CDS             3645..4418
                     /locus_tag="KMT45_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003948.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSETSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAKLYTEKVEQFFKSGANKDFSKISDYSENFFNETQDEIMNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLEKPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEVEMVIKIF"
     gene            complement(4648..4896)
                     /locus_tag="KMT45_000462"
     CDS             complement(4648..4896)
                     /locus_tag="KMT45_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBE7003949.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(4995..5345)
                     /locus_tag="KMT45_000463"
     CDS             complement(4995..5345)
                     /locus_tag="KMT45_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003950.1"
                     /translation="MIFENESEEIGRMTEENKVDLLETLVINKKEFEQLFLDFFVQYD
                     RIYDFNSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDADYYQPQEVFR
                     EFRNKIIGFSQTTK"
     gene            complement(5386..5880)
                     /locus_tag="KMT45_000464"
     CDS             complement(5386..5880)
                     /locus_tag="KMT45_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003951.1"
                     /translation="MFKVYFPFQWLVKGIFFVLLGLILFGSYLTIKARYFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESGKSETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFNL"
     gene            complement(5873..6175)
                     /locus_tag="KMT45_000465"
     CDS             complement(5873..6175)
                     /locus_tag="KMT45_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003952.1"
                     /translation="MSDPYIKVYCSKETKKLFDSFYEKNKGTLGTKANMFRVMVSNLP
                     MLASQSNKKFSDPESIKLEQTISELESSIMKKLDGIDQKLSCSLKTKYKTEEKKDV"
     gene            complement(6198..7535)
                     /locus_tag="KMT45_000466"
     CDS             complement(6198..7535)
                     /locus_tag="KMT45_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBE7003953.1"
                     /translation="MGSIIKIASKSRSIHNTISYMLNKQEDSELVTGIGVSNDLNAIE
                     EFKRTNEIFGDKGKIKLWHFTQSFDPLEDISAEKAHELGVKWAEKMFPNQSILVVTHT
                     DKGHVHNHFGISPNDQLTGKKMDIRKNDIRVLQAWNDNILIDNGINPLDMYKYNHLVA
                     DRENWKEREFRTENKISNREKIKLAVSEVAQYAKNYDDFHAELKKNHSIETYRFAKNQ
                     RIGYKLLDSEGELILKIGGKRLGQNYDMNYLDDLFTENKKAEQIQSKEVLTEIAIRHL
                     GKSLNGRADSRFNWQERAQRFELDNENIPFLRSSKDYEEFYDKYKFEIEDRIENLLDV
                     FGENKNQFFGFAVEEEPLKYKKKCVNLVYKKVVYDIAESLSTDYDQFNIQDTEPALKR
                     NFKKRYIQETKRVLYLFNRAIDDGTIEKARREYEETARGIDYQKQQNQYDMDF"
     gene            complement(7520..8017)
                     /locus_tag="KMT45_000467"
     CDS             complement(7520..8017)
                     /locus_tag="KMT45_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003954.1"
                     /translation="MAIDVSKLPLEERRKRYKRNEDSRRQNRPIKFRVDDYQYQELEK
                     TADIFGLSVPMYVKKIAVDNAGKSLKYTDRQYKEINFHLLAIGRNLNQLVKTANYLRV
                     QLNKKDTEIQKEEHQSVYLSFTTKQERLKRLYAEKNDLMIQIKSISDPLNRLEKELNK
                     LWARL"
     gene            complement(8166..8504)
                     /locus_tag="KMT45_000468"
     CDS             complement(8166..8504)
                     /locus_tag="KMT45_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003955.1"
                     /translation="MARKKQKSIADLKKELAELERKLYPELGKYVYEKTGISSVKEFE
                     ETFEINQKGTQEKWQKAVNFANSEFTLVHEFLASDQLMETKFQQFLRRKGETAYRIFY
                     REEAQEESVR"
     gene            complement(8508..8693)
                     /locus_tag="KMT45_000469"
     CDS             complement(8508..8693)
                     /locus_tag="KMT45_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003956.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     MMKNELMNELSHIQKGA"
     gene            complement(8707..11259)
                     /locus_tag="KMT45_000470"
     CDS             complement(8707..11259)
                     /locus_tag="KMT45_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003957.1"
                     /translation="MKIIKKKIDNRKINWLLISFLILIAMIIFTSNVAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLNTVVIGLMVLSLVIIGYKMVTGKGTVDLKSVGMNVVMSIVLI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     MIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENSTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISTNIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNSGGNDQTEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMAS
                     ERATKPFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPIVAKNKEDETGKYADSMPI
                     RLSDRKDGAKIENADRIMSSSERMREAMKNIAESNTNYASAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKN
                     SNTGLEKSVKENLQNSTSSQDRRSVDVRENLHNTTSSQSGNRTVDVRENVQSSSTGQT
                     RTVDVRLNLQDTTSSQSGNRTVDVRENVQSSAPNQPKTVEVRETRAEASQGDKTQKIN
                     IVEERKERPNEKVIVEQEVKEKKRQRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            complement(11246..11464)
                     /locus_tag="KMT45_000471"
     CDS             complement(11246..11464)
                     /locus_tag="KMT45_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003958.1"
                     /translation="MIENHLVDKIYCLDHNADTLELIESICSNTIVLDLRAVNDDYVT
                     HIIVNKKMAQKLSESLKKWAEGGNNENN"
     gene            complement(11457..12197)
                     /locus_tag="KMT45_000472"
     CDS             complement(11457..12197)
                     /locus_tag="KMT45_000472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003959.1"
                     /translation="MREKLKKLGDKKRHTFQGTFEFNGYKKDKYKFGQEYENATFMLS
                     NLKIVQGDNLELVTDHIWMNLTKQFLKFGWLKKGDMVQFDGRIKSYSSKKGINYKIER
                     PSKVKVFRKGTEINESSALKISTRYEIVEDIKRQNREYYEARDFFFENYFSVPRYFKL
                     KEKWPEIQAVVAINENRYTSEQFKQVFKDRDSLVDLYKKIQETDKYNLQKEFYNGLVS
                     NKNRIDLNEVEGYLHKIENFDYGRGYFD"
     gene            complement(12330..13205)
                     /locus_tag="KMT45_000473"
     CDS             complement(12330..13205)
                     /locus_tag="KMT45_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HBE7003960.1"
                     /translation="MAEKRMLAKSFLNNASFMKMSDSAKLLYVYLNTNADDDGIVEAY
                     PYTKMLGTPDDDVKILFTKGFVFFLNEDLLAFLPHWLQNQNIRIDRYKPSIYRFKLLN
                     LKPEFFSKLKPIKFDMILQSGQVEPLRVDLMEKWYPAGIPCDVKSDTQLSQDQNSQDQ
                     TNQDKDSIEEINQDQNSFFENIINQKGIQIITRLGDRYTEVQREKYLKLIASMKDSIE
                     REYKIEIRGQDYFDITNKMLTRLFNSINEKLNLGEEIDEMKFINTTARNHWEDIAKQE
                     KASRLDMLETLKKIG"
     gene            complement(13375..13656)
                     /locus_tag="KMT45_000474"
     CDS             complement(13375..13656)
                     /locus_tag="KMT45_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7003961.1"
                     /translation="MEQIGVSVMLNTDQTNQLKTHVFEVTKEAIDQARIDSGLERPFL
                     KGKEMAKFLNVSYGTFLKFKRLGLPIIVVDTIELYSKEEVKKWMLQHQI"
     gene            complement(13711..13908)
                     /locus_tag="KMT45_000475"
     CDS             complement(13711..13908)
                     /locus_tag="KMT45_000475"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7003962.1"
                     /translation="MFQINEDFSIRIRERRGKLMITKTKAAELIGISRQTLKKIECFE
                     VQSVSKNVYQKIIGWLLKSDY"
     gene            14075..14449
                     /locus_tag="KMT45_000476"
     CDS             14075..14449
                     /locus_tag="KMT45_000476"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7003963.1"
                     /translation="MTQKNRIKETREEQNLTLKELSNLSDIPYQTLRNYEIGKRKPRN
                     DEVWWKLGTILNVRGPYLKGEDSFKTNDELHIYYLKKSHTNFELSKKEEIIDIIDKLN
                     EDGLDEALRQLALLSKIDDFKR"
     gene            14539..15705
                     /locus_tag="KMT45_000477"
     CDS             14539..15705
                     /locus_tag="KMT45_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE7003964.1"
                     /translation="MSIQKYKTKSGTRYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDMGYHRPSNDLFRTVYEEWLPSYKNRVQETTYQRTKDLFRLHILPKFG
                     DKSISKITPIYCQNAINEWAVKYTNIKQLKSYSSQIFEYAIFAELLQRNPMTNTKLPK
                     QEKKESENYFSLEELKEFLEILNKEESLKHILIFQLLITTGVRKGELSALRWADINFE
                     EQLLHIGKSYATIRTEDPNATRKTIRIQKNTKNVSSERILPIDTQTIEMLKLWKKEQA
                     EVLLQFGINANTKKQLIFTYVNANGDVNQPLHADYSNNIMRRLEKKYKFKHVTIHGLR
                     HTHATLLLEGGASIKEAQDRLGHKNAETTLNTYSHVTEKAQRNAVDKFIAYTGL"
     gene            complement(15804..16196)
                     /gene="rpsI"
                     /locus_tag="KMT45_000478"
     CDS             complement(15804..16196)
                     /gene="rpsI"
                     /locus_tag="KMT45_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBE7003965.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16212..16655)
                     /gene="rplM"
                     /locus_tag="KMT45_000479"
     CDS             complement(16212..16655)
                     /gene="rplM"
                     /locus_tag="KMT45_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBE7003966.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(17174..17641)
                     /locus_tag="KMT45_000480"
     CDS             complement(17174..17641)
                     /locus_tag="KMT45_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBE7003967.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(17761..19299)
                     /locus_tag="KMT45_000481"
     CDS             complement(17761..19299)
                     /locus_tag="KMT45_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBE7003968.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            19379..20065
                     /locus_tag="KMT45_000482"
     CDS             19379..20065
                     /locus_tag="KMT45_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBE7003969.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(20266..20793)
                     /gene="msrA"
                     /locus_tag="KMT45_000483"
     CDS             complement(20266..20793)
                     /gene="msrA"
                     /locus_tag="KMT45_000483"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBE7003970.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(20877..21554)
                     /gene="pgmB"
                     /locus_tag="KMT45_000484"
     CDS             complement(20877..21554)
                     /gene="pgmB"
                     /locus_tag="KMT45_000484"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBE7003971.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(21551..22681)
                     /locus_tag="KMT45_000485"
     CDS             complement(21551..22681)
                     /locus_tag="KMT45_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBE7003972.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(22681..24864)
                     /locus_tag="KMT45_000486"
     CDS             complement(22681..24864)
                     /locus_tag="KMT45_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBE7003973.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(24861..25886)
                     /locus_tag="KMT45_000487"
     CDS             complement(24861..25886)
                     /locus_tag="KMT45_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE7003974.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(25931..26758)
                     /locus_tag="KMT45_000488"
     CDS             complement(25931..26758)
                     /locus_tag="KMT45_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBE7003975.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(26755..27822)
                     /locus_tag="KMT45_000489"
     CDS             complement(26755..27822)
                     /locus_tag="KMT45_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBE7003976.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(27848..28684)
                     /locus_tag="KMT45_000490"
     CDS             complement(27848..28684)
                     /locus_tag="KMT45_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7003977.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(28681..29550)
                     /locus_tag="KMT45_000491"
     CDS             complement(28681..29550)
                     /locus_tag="KMT45_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7003978.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(29620..30903)
                     /locus_tag="KMT45_000492"
     CDS             complement(29620..30903)
                     /locus_tag="KMT45_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBE7003979.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(30917..32602)
                     /locus_tag="KMT45_000493"
     CDS             complement(30917..32602)
                     /locus_tag="KMT45_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBE7003980.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            32877..33902
                     /locus_tag="KMT45_000494"
     CDS             32877..33902
                     /locus_tag="KMT45_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7003981.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(34497..38171)
                     /gene="rpoC"
                     /locus_tag="KMT45_000495"
     CDS             complement(34497..38171)
                     /gene="rpoC"
                     /locus_tag="KMT45_000495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBE7003982.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(38229..41846)
                     /gene="rpoB"
                     /locus_tag="KMT45_000496"
     CDS             complement(38229..41846)
                     /gene="rpoB"
                     /locus_tag="KMT45_000496"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBE7003983.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            42574..43542
                     /locus_tag="KMT45_000497"
     CDS             42574..43542
                     /locus_tag="KMT45_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE7003984.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            43560..44465
                     /locus_tag="KMT45_000498"
     CDS             43560..44465
                     /locus_tag="KMT45_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7003985.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            44462..45268
                     /locus_tag="KMT45_000499"
     CDS             44462..45268
                     /locus_tag="KMT45_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7003986.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            45404..46393
                     /locus_tag="KMT45_000500"
     CDS             45404..46393
                     /locus_tag="KMT45_000500"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBE7003987.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(46710..47234)
                     /locus_tag="KMT45_000501"
     CDS             complement(46710..47234)
                     /locus_tag="KMT45_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBE7003988.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            47521..>48703
                     /gene="scm"
                     /locus_tag="KMT45_000502"
     CDS             47521..>48703
                     /gene="scm"
                     /locus_tag="KMT45_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBE7003989.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SA"
BASE COUNT        14097 a        10148 c         8302 g        16156 t
ORIGIN      
        1 tcttactctg tcttactcta tcttactctg tcttactcta tcttactctg ttttactcta
       61 tttaaagaat agtattgagt cggatctttg gtactagtac catgccactg aattattttt
      121 ttccttaaca tttcctttaa taccttacgg ctatatccga cccctttttt taatacttct
      181 gaagcttctt ttaccgaaat tttttcgtta ttaaataaat gtgaaacaat catcttctca
      241 gaagtagaca aagagtctac ttcaaattct ccaagataat catctagtct gtctctctct
      301 cttaaatgtc tagaggttat actattttct aagactaata aaaccgaatt attatttggc
      361 tcactatatt taggttcatt taaaaatagt aattgcattt cgtcatatat tcgattcaca
      421 ccttcattaa gttccttaac ccatccgaat tcagataatg tcctagcgat tctgggattc
      481 ctagcatacc tagttcggcg catattttgt aaagtgacta tattaggtaa agctcccggg
      541 ctaaaaattt ctaacctatc atcatacata ttaattctaa cgtaatcccc tttataagaa
      601 tagtctctat gcgttaaagc attgactata ccttcaaacc atgcaaattc aggatattca
      661 ggtatgatac taaactttcc atcatcattt aaatactgaa attctcttag ttgcgttttt
      721 acatccctag ttatagattc gattagcttt ggaattgcac cttcatagtc aaattcttta
      781 actatattta atctccgtcc agtttcatta cgcttaccat catatctcaa aaaacgaatt
      841 ctagaattag gtaaggctag aaatgtattc ttaccaaata gaagcatgcc agcatttgtc
      901 aagtgaccat ttctcattaa ccctcttcca gtgagtatgg tttctgtgga tatcgcttca
      961 gcttttttta attttttata tgaatccatg agagattcat caatatcatc aagagtactg
     1021 tctaaaattt ctatatcttc aaacattctc tgtcctttgt cgtattctag tttggatatt
     1081 tggtcatgag tttgatgaac acttttatcc ccaattctca agaaacaatc accgttcgaa
     1141 ttaaaaatca catggcttat gctaggttca acgtctataa tgaataatat atcgtcttct
     1201 cctctagcat tcttaatact cttcatttct tttttaaaag ttggattggg ttttgtcatt
     1261 gaatttatag ctagtacata ttcatctgga tcgatcgcac cttgaaattt aaacccagaa
     1321 atagtcccat catcttctat accgattacc agcttacctc catcggcatt tgcaaacgct
     1381 actatgtgtt ttataatatc tctaggctta atccgtgcac tttttctatc aaaatactga
     1441 tcttcttttt cacttataaa cttttgaatc tcataatcca tttaaaattt cccttcttaa
     1501 atttttttat tatagagccg tatattcaat ttccacaaaa aaataatcta aaacaaaaca
     1561 gacaccttca ttttgatatg ttctcccaca tatgaatcgg tcgctataca gcattacata
     1621 tagtaattat tcatactgcg aaataaatag ctccaaatct ttagctggcg tagttaagct
     1681 taaattcgaa atgaaagttt cattgactat tccactttca tcaaatccct tcttcaacca
     1741 atcagagttc cacaacgtaa atcctttagg attagaaact ttcttacata attttgcact
     1801 ataggatttc tctcttgaat gaattaaaga aagtctttgt aaccaaattt caactatatc
     1861 cgtattagga gtattaacaa attttcttaa gattttatcc aagtataagt tgatttcttc
     1921 tacagcaata aactcaaaaa gcttacctaa aatcacaata caagcctcca aagttctagg
     1981 attcttttgc attatattaa caagaatact aatcaattgg tagatatcat ctggtctttt
     2041 gtttaaagta gaaactctat gtgtatagac ttcacttagc gctttactta aactcccgca
     2101 atttggatat ttgtctccta aaagctttac ctgtagcaaa tgcttttgaa ggctaagctt
     2161 aaacttccac ttgttattac ttttctctgt aaaacttgca tgtttaagat cccagtaaat
     2221 tttatcaggc ttaattgcat ctaaaattat attatccgat aagaatgttt tatttgaatt
     2281 tagcttcaaa tttagatccg ataatacttc agataacaat ctgataatca cttcagcttg
     2341 atcttttcga accgaaaaaa ttctataatc atctctgtaa cgaagtattt tgaactcttt
     2401 aatctctgtt ttttctatct tgttcagtaa cattttatca gcatacccaa gtactatttc
     2461 agctacaaaa tccatcaata catttccttg aggtataccg ttcgtttgtc cattttgtaa
     2521 atatcgtatt ttagaatcta ttaagtttcc aacgccttga gtatcttttc tatgtgtctt
     2581 agcccattct tttgaatgta ctgcccacgg gatagtatgt gtgtacattg agctataaca
     2641 atcggtaata tctgaatgaa tgcagtagtc atactccaaa gcataactaa tctgtgcttg
     2701 ttcaaggttc tcccaccaat ttaaaatagt ttccgcagta tcgctctttg aagaaataga
     2761 ttccacagga agtgatatac attcaattcg cttgtcggct tggaattctt caaacttatt
     2821 tttgatgtga ttccaattat ttttctgtgt aatcccgttt actaagtcta catatactaa
     2881 tggatgtaat atttgaaggg gtctccaagc ataattacca tctttattag ctaatattcg
     2941 atgattaaca tcaactgcct cagagagcga tctcttatgt ctaactagtt cagtaaatga
     3001 tttgtttttt aataaatcca ctgctttctc aatgcctact gataaatcaa tataattggg
     3061 caacgatgta ctaaaaaaac ttgatgtttt taaaaagaat tgctttgctt cttgatgatt
     3121 catctctaaa atactttttc tagtcctagt caaattcaaa atctcctaga tcaattaagt
     3181 tgtaatgtaa gaggataccc taacgttaca tatctcattc attattatac cagtatttct
     3241 tcatgacaca taagggtgta acataataat tttaaacgaa atgactacaa gtagtcattg
     3301 aagtaagtct tcataagata cctgtaggat atccctaatt gctctcaatt gatctaactt
     3361 aatgtgttgc cggccacctt caattttaac caatgtttcc ctagttatag caattttatg
     3421 aagctgtagg tctctaatca gctcggtttg accaattcct ttttctaatc ggattcgtct
     3481 aatattgcta ccgattacat tttctttatc aataattttt tcctctttca agaatagcac
     3541 accttttgga ctaaaattag tcctttttct tattaatctt aaagattcgg tatgctacaa
     3601 tgggactagt attagtccac attcaatttt aaggaggaat ttttttgaag aaatttgcaa
     3661 tcattctaac ctgcgcttta ctcatcggaa ctataagcgg gtgtaccact tctgaaacaa
     3721 gttctacaag tacggaaact agtacaacaa cctcagaaaa gaattaccca atacattttt
     3781 ttccggttcc agatggattt gaagcttatt atcatgataa aaaactagat gtccctttat
     3841 cagaaactga atctactgaa aagattttgc caattgaaat tgaaagaaat caagacgata
     3901 taaaactaaa agctgatctt cccttactga acagcacaca aattgataga catgaaaata
     3961 ttaaagttaa tattgaaaaa ggagcgttag caaaacttta tactgaaaaa gttgaacaat
     4021 tttttaagtc tggtgccaat aaagactttt caaaaataag cgattattcg gagaactttt
     4081 ttaatgaaac ccaagatgaa atcatgaact caacttttat ggctgaagat atgtacaata
     4141 tgacattaaa aaaaataaaa gcttacgacc atccgttaaa tctgacacta aataatggac
     4201 tgctttcttt tgtattagca ggttacgttg actatgaata ttcaccagat tatttggaga
     4261 aacctcttca acacgagaac aatgatatta attttagatt tgtctacgag tcaaaaagta
     4321 aaacatggtt agttgatcaa ttaagcacga taaacacaag cttccatgaa ttacctaaac
     4381 ctgaaaatga agttgagatg gtcattaaaa tcttctaaaa aagaagcgtt gaaggagaaa
     4441 tattcctttc aacgctcttt tttatatgct tagaaacata aataaattgt atactttctt
     4501 tttagcccct attacttttc acgaaaaata tctttttttc gacgctagcg tttctctact
     4561 tctaatactg tacacagttt ttgtattatt gaaaaattac tcattattta gcattaatcc
     4621 acatcgtttt atcaatcgac cttcatacta aactacttta aagtcatctt ccttctcgtt
     4681 tttcattaaa tcagacacat aagcccatgt acccgttcgt tgtaaataaa gttcttcttg
     4741 taacaactcc cattttctct tagaaatcat aatgacgcca tcgttcaatc catttttaga
     4801 atccctagta atttctattt cagaatttga atgaactact tcttgcaaca ctttacctat
     4861 attttctttg aagtttgttg gagtaatagt atccaaaatg aacacctctc ttctctatac
     4921 ctctattatg aagcgcccat caaataaagc aagcgttgag gaataattct atcaacactt
     4981 gctttattaa gctttcattt tgtagtttga ctaaaaccaa taatcttatt tctaaactct
     5041 cgaaaaactt cttgtggctg ataatagtct gcatctattt caaatgtttc tgagaataat
     5101 tcattgaatt tttctactga aataatgatt ttaccattct tctctcgact gttttctatt
     5161 atttctgctt ctaattcaaa taaatctagt gacgaattaa aatcataaat cctgtcatat
     5221 tgaacaaaaa aatcaagaaa aagttgctca aactctttct tatttatcac tagtgtttca
     5281 agcagatcaa ctttattttc ttcagtcatt cttccaattt cctcactttc gttttcaaaa
     5341 atcacaaaaa tgatattaac ttaaaagcca ctaacaaaat aagacttaca aattaaaggg
     5401 taatccaaca aaataaccat tcgctctacg tactacgatt tggtagtcct gcctttcaat
     5461 cggtaattca taatagtaat ttactgtttc tgatttacca ctttcaatat actcaggatt
     5521 atccaatatc atagcatact cataaagctc ttcaccatcc ttattttcaa tcgaccattg
     5581 ttgcggacgt atcggatttt ctgtatcatt tttcattgta taggtcactt gtaatacacg
     5641 atcatgctgt gcaaattcat ttcttttttc ggtcagttca atatttttaa atgttagttc
     5701 aaccccatct gcctgataag tttcacctat ttttaaatct tttgttacaa tttttattgg
     5761 cttttcttct ggaaaatacc tggctttaat tgttagataa cttccaaaaa gaatcaatcc
     5821 tagtagcaca aaaaaaattc ctttaacgag ccactgaaac gggaagtaaa ctttaaacat
     5881 cttttttctc ctccgttttg tatttggttt ttagagaaca cgataacttt tgatcaatac
     5941 catccaactt tttcattata cttgattcta gttctgaaat tgtttgttca agtttaatgc
     6001 tttcaggatc actaaatttt ttatttgatt gactagccaa cattggtaaa tttgaaacca
     6061 tcacacggaa catatttgct ttcgttccaa gagttccttt attcttctca taaaagctgt
     6121 caaacaattt ttttgtttcc tttgaacaat atactttaat atacggatca gacattcttt
     6181 tccctctccc tgtttactta aaaatccata tcatattgat tttgttgttt ttgataatca
     6241 attcctcttg ctgtttcttc atactctctc cgagcttttt caatcgtacc atcatcaatt
     6301 gcacgattaa ataaataaag aacacgtttt gtttcttgaa tataacgctt cttaaaatta
     6361 cgttttaaag cgggttcagt atcttgaatg ttaaattggt cataatcagt tgataaactc
     6421 tctgcgatat cataaacaac ttttttatac accaaattta cacatttctt cttatatttt
     6481 aaaggttcct cttccactgc aaatccaaag aattggtttt tattctcacc aaaaacatcc
     6541 aataaatttt ctattctgtc ctctatttca aatttatatt tatcataaaa ttcttcataa
     6601 tcttttgatg atctaagaaa tggtatattc tcattatcta attcaaagcg ttgcgctcgt
     6661 tcttgccaat taaatctact atccgctcta ccatttaacg atttaccaag atgtcgaata
     6721 gcaatttcag tgagtacctc tttagattga atttgctccg cttttttatt ttctgtgaac
     6781 aaatcatcaa gataattcat atcatagttt tgccctaaac gctttccgcc aatttttaag
     6841 attaactcgc cttcagaatc taagagtttg tacccaatac gctgattttt ggcaaaccgg
     6901 taagtctcaa tagaatggtt tttcttcaat tccgcatgaa aatcatcata atttttcgca
     6961 tactgagcaa cttcagaaac agctagtttt attttttctc gatttgaaat cttattttca
     7021 gttctaaatt cacgttcttt ccaattttca cgatctgcaa ctaaatgatt atacttatac
     7081 atatcaagtg ggttaattcc attatcaata agtatattat cattccaagc ctgcaaaact
     7141 cgaatatcat tctttcgaat atccattttc tttcctgtta gctgatcgtt tggcgaaata
     7201 ccaaagtgat tgtgaacatg ccctttgtct gtgtgtgtga caaccaaaat tgactggtta
     7261 ggaaacattt tttctgccca cttaacaccc aactcatgtg ctttttcagc ggaaatatct
     7321 tctagtggat caaagctttg tgtaaaatgc caaagcttta ttttcccttt atcaccaaaa
     7381 atttcattgg ttcgtttaaa ttcctcaata gcatttaaat cattcgatac gccaattcct
     7441 gtcacgagtt cagaatcttc ttgtttattt aacatatatg aaattgtatt atgaatactc
     7501 ctactttttg aagcaatttt tataatcgag cccataactt attcaactct ttttccaagc
     7561 gattcaatgg atcgcttatt gattttattt gtatcattaa gtcatttttt tcagcatata
     7621 gccgctttaa tcgctcttgc ttagttgtaa aagataagta aacactctga tgttcttctt
     7681 tttgtatctc ggtatctttt ttatttaact gaacacgcaa ataatttgct gttttcacta
     7741 attgattcaa atttcttcct attgctagta aatgaaaatt aatttcttta tattgtcgat
     7801 cggtatattt tagacttttc cctgcgttgt ctactgcgat tttcttcacg tacattggaa
     7861 cagataaacc aaaaatgtct gctgtttttt ctagctcttg gtactgataa tcgtctactc
     7921 gaaactttat cggcctattt tgtcgtcgac tatcttcatt acgtttatat cgctttcgcc
     7981 tttcttctaa tggtagttta ctgacatcaa ttgccatgcg cacttctcct tttcaatatt
     8041 atttttaagt tcaaaagaag caagcgaggg gaaagggaac ccttccccgc ttgtcgaggg
     8101 agtaaactcc ctacgagcac cctcatgctc cgcattttat cgagattaga tagccgataa
     8161 atcagctacc taacactttc ttcttgcgcc tcttctcgat agaaaattcg atacgctgtc
     8221 tctccttttc ttctcaaaaa ttgttggaat ttcgtttcca tcaattgatc gcttgcaaga
     8281 aattcgtgta ctagggtaaa ttcgctattc gcgaaattta ccgccttttg ccatttttct
     8341 tgagtacctt tttgattaat ttcaaaagtt tcttcaaact cttttactga tgaaattcct
     8401 gttttttcat agacatactt tcccagttcg ggataaagtt ttctctccaa ctcggcgagt
     8461 tcttttttca aatctgcaat tgacttttgt tttttccttg ccatttttca tgctcctttt
     8521 tgtatgtgac taagttcatt cataagctca tttttcatca ttaattcttc ttgaatcatc
     8581 agttcaatat aatttgtgac cgtgattttt tcatttgttg ctttctttac tgattcacat
     8641 aattcgtcaa gttctaagaa atcatgtgta ctaatctgta gaattaattg catgacaatc
     8701 acctctttat tttttaaata gactatcaaa cagcggattt ggtttatcag aaatatcctg
     8761 aaacagtgga ttattttcat aagtaaatcg ttgacgtttc ttctccttca cttcttgttc
     8821 tacaatcact ttttcgttag gtctttcttt tcgttcctcg acaatattta ttttttgcgt
     8881 cttatctccc tgacttgctt cagctcttgt ttcacgtact tctaccgttt ttggttgatt
     8941 tggcgcacta ctttgtacat tttctcgtac gtctactgtt cgatttccac tttgactact
     9001 tgtagtatcc tgtaagttta atcgaacatc taccgttctt gtttgacctg tactgctact
     9061 ttgcacattc tctctgacat ccactgttcg atttccactt tgactacttg tagtattatg
     9121 caagttttct cgaacatcta ctgagcgcct atcttgacta cttgtagaat tttgtagatt
     9181 ttctttaacc gatttttcta atccggtatt tgaattttta gcttcttgga ttctttcttt
     9241 ggtcaacgct tctttttcgt ccatattagc tcctgtacgc ccttctacac gtttccttaa
     9301 catttcctca cgattcattg cttcaggcgt gtattttgct tcctgtcgct tctgattaat
     9361 taattcttca gcagacttag cttgaccgtg catagcattt ttacgttctt caatatcttg
     9421 ttgaactttt tgctgaattg cacttgcata attagtattt gattcagcaa tatttttcat
     9481 tgcttcacgc atacgttcag aactagacat aattctatcg gcattctcaa tcttagcccc
     9541 atcttttcta tccgaaagac gtattggcat agaatcagca tattttccag tttcatcttc
     9601 tttgttttta gctactatag gtttgctatt tttattaata gcggacacgg taccatcatc
     9661 taaaccttct ttgaatttat tcttggtatc attagccata tcacgcatac tcttaaatgg
     9721 ttttgttgct ctttcactag ccattgtagc tccatccgaa gctaagcctg ataatccacg
     9781 ttcacgagca tacccaagcg ttttccccgt tttattagct ttttgagaaa gtgattgacc
     9841 aaagttgcgt tcaggttttc gttcttcccc ttccgtttga tcgtttccac cactattttt
     9901 cccaccttta gatcggttca tgccacctgt tcctttacga aacagcattg cacccattcc
     9961 aaaagcagaa gcaagttgcc catagccttc tttgagtcca atatccacac caaagtaacg
    10021 catgactgtt ccactgcctt tgattaaaac aaacacagca cagatataag caattgtttt
    10081 tataaaaata tttgttgaga taccttcgcc agtatttaag taatttatga acattgcaaa
    10141 catagataat ccaaaccctt gaaatccgac tgtcaaatag cactgtaaaa tatctgacaa
    10201 aacttgtttt gatcgttgtc ctgtttctaa gtcagtagca aatactaaaa ctccgagtac
    10261 acgtttgaat gctagctcga gtattgctgt aattataatg aaagcactaa aaacatacgc
    10321 taccgctaaa gcaatcaatc caaatgaaat tccccacagg ttcgcttgat agcgataata
    10381 tcctgatttg ataccatcaa caaaggttga caaaaaatta tctgaaaatt tagtagcaac
    10441 aaattcatta tcagaatttt caactaattc ataacgcagg ttatcagctg ttgagttctc
    10501 tttttccatt ttatcaatta cattatctgt caatacttgc gtaagatccg ttttccaaaa
    10561 attttctttt gttagattgt tccgatcatt cgttttatcg atcatggaat attgatccgt
    10621 tttattgata taagctaaat cagtaacccc ttgttcaatc agcgaccatg ccacaccgcc
    10681 atcttgattg ttaatactag ttgcgtcatt ataaagagtc tttgcaaaca ttattcctga
    10741 aagtttttct gttgatccaa tcaaagtcgg cattaaaaga attaatacta tcgacattac
    10801 aacattcatt ccaacagatt ttagatcaac tgttcctttt ccagtcacca ttttataacc
    10861 aataatcact aatgagagta ccattaaacc aataacaact gtatttaaaa ctgactgata
    10921 gactttgttc aatccagcac tttccacaaa gctaaacaaa tctaaaacct cgggaatcat
    10981 attttgaatg ccatcgacaa cagcgaacat tgattttata acagaccaac ctatccacat
    11041 accgaactgg ctgtaaaaat ttccgttata ttttttaaga taattctgat aaagtggatt
    11101 agttaaaaac tcagtttgac tctcagtatc atcaaaccag ttgaaaatac tgccttctgc
    11161 aacgttactc gtaaaaataa tcatagctat gagaataaga aaactaatca acaaccaatt
    11221 tatttttcta ttatcaatct tttttttaat tattttcatt gttacctccc tcagcccatt
    11281 tttttaggct ctcagaaagc ttctgagcca tttttttatt aacgatgatg tgagttacgt
    11341 aatcatcatt aacagctctt aaatctagaa caatagtatt tgagcagata ctctcgatta
    11401 actcaagagt atctgcgtta tgatcaagac agtaaatctt atctactaga tgattttcaa
    11461 tcaaaataac ccctcccgta gtcaaagttc tcaattttat gaagatatcc ctctacttca
    11521 tttaaatcaa ttctattctt attgctgact aacccgttgt aaaattcttt ttgtagatta
    11581 tatttatctg tttcttgaat ctttttatat aaatcaacca gggagtccct gtctttaaaa
    11641 acttgcttga attgttcgct tgtatatcgg ttttcattaa ttgcaacaac tgcttgaatt
    11701 tcaggccatt tttctttcaa cttaaaataa cggggaacgc tgaaatagtt ctcaaaaaag
    11761 aagtctcgtg cttcatagta ttctctattt tgtcttttta tatcctcgac aatttcgtat
    11821 cgtgtagaga tcttcaaagc tgaactttcg ttaatttctg ttccttttcg aaagactttc
    11881 acttttgaag ggcgttcaat tttataattt atcccctttt tacttgaata acttttgata
    11941 cgaccatcaa attgaaccat atctcctttt tttaaccacc caaattttag aaattgttta
    12001 gttaaattca tccaaatgtg gtctgtgacc aactctaagt tatccccttg aacaattttt
    12061 aaattcgata acataaaagt agcattttca tactcttgac caaacttgta tttatccttt
    12121 ttatatccgt taaattcaaa ggtaccttga aatgtatgcc gtttcttatc tcctaatttt
    12181 ttcaattttt ctctcaaaat aatcaactcc atcatttaat atatttttgt gatggtagat
    12241 actctcgatt aacacaagag tatctgcgtt gtaatcaaga caataaattt gatatattaa
    12301 ccgtttttca atcatattat taatcctttc taacctattt ttttcagtgt ctctagcata
    12361 tccagtctac tagctttttc ttgcttagct atgtcctccc agtggttcct agctgtggtg
    12421 ttgataaact tcatttcatc aatttcttca ccgagattca gtttttcatt tatagaatta
    12481 aaaagtcttg ttagcatttt gtttgttata tcaaaatagt cttgacctcg aatttctatt
    12541 ttatattctc tttcaattga atctttcata ctagcaatca acttaagata cttttctctt
    12601 tgaacttcag tatatctatc acctagtctg gtaattattt gaattccttt ctgatttata
    12661 atattttcaa aaaaggaatt ctgatcttga ttaatctctt ctatactatc cttatcttga
    12721 ttagtttgat cttgactatt ctgatcttga cttaactgtg tatccgattt gacgtcgcat
    12781 ggtataccag ctggatacca tttttccatc agatcaactc ttaatggttc tacttgacca
    12841 gactgcaaaa tcatatcaaa ttttattggc tttagctttg agaaaaattc tggttttaag
    12901 ttcaataact tgaatcgata aatagaaggt ttatacctat cgattcgtat attttgattt
    12961 tgtaaccagt ggggtagaaa cgctaacaaa tcttcattta aaaaaaatac aaaacctttt
    13021 gtaaaaagta tctttacatc atcatcgggt gttccgagca ttttcgtgta tggataagct
    13081 tcgacaattc cgtcatcatc tgcgtttgtg ttcagatata cgtataataa ttttgctgaa
    13141 tctgacatct tcataaaact tgcattattt aaaaatgatt tagccagcat tcgtttttct
    13201 gccataaaaa tcacacttct tcctatttga atgttaaaat gtagtcaaat aaagtttagc
    13261 tagaacttta ttattgagcg ttcctagtgg tctgcaaact aacgggaaca ctctttttta
    13321 ttcttattaa aaccagcaat actagttcac cttctttcat ccttttacat ccaattaaat
    13381 ttgatgctgt agcatccatt tttttacctc ttcttttgaa tatagttcaa ttgtgtcaac
    13441 cacgataata ggtaatccta accttttaaa cttcagaaaa gttccatatg atacgtttag
    13501 aaattttgcc atttcttttc ccttaagaaa aggtcgttct aatccgctgt ctattcttgc
    13561 ttgatcaata gcttcttttg tcacttcaaa cacatgtgtc ttcaactgat ttgtttgatc
    13621 agtattcaac attacactga ctccgatttg ctccattaaa aattcccact ttcatatttt
    13681 catcaataag ccaactcgcc aagttgtttg ttaataatct gatttcaata accaacctat
    13741 tattttttga taaacatttt tcgacacact ttgaacttcg aagcattcaa tttttttaag
    13801 agtttgcctt gaaattccaa ttaattctgc tgctttagtt ttcgtaatca tcaactttcc
    13861 tcgtctctct cgaattctta tactaaaatc ctcattaatt tgaaacataa acatccctcc
    13921 atttttattt ttgcatttat ccgtttatcc gatgtcggat aaatagattt tagtatatat
    13981 tattttgata gtcaattaaa aatatacatt ttggataaaa ttgttcattt ttttatccaa
    14041 atcatatatt atagatagta agaggtgaac ttcattgact caaaaaaaca gaattaaaga
    14101 aactcgcgaa gaacagaatt taacacttaa agaacttagc aatctgtcgg atattccgta
    14161 tcaaactttg agaaactatg agattggtaa aagaaaaccc agaaatgatg aagtttggtg
    14221 gaagttaggt acgattttaa acgtccgtgg gccctactta aaaggagaag atagttttaa
    14281 aacaaatgat gaattgcata tttattatct aaaaaaaagt catacaaatt ttgaactgtc
    14341 taagaaagaa gaaattatag atataataga taaactaaac gaagacggtt tagatgaagc
    14401 cttacggcag ttagcactat tatcaaaaat agatgatttc aagcggtaac ttacttatca
    14461 tcatgtagac tatcaggtat cataaactaa ttcctcaatc agccaactcg ccaagttatc
    14521 agattggaga gattagttat gtcaatacaa aagtacaaaa caaaaagcgg tacacgatat
    14581 agtgtccgct tatatgttcg taaagatgaa aatgggaagc aagaatacta tgtaaaaaaa
    14641 ggcttcaaaa ctgaaaaaga agccaagtta cacgaagcaa gaaaaaaggt cgagattgaa
    14701 gatatgggct atcacagacc ttctaatgac ttatttagaa cagtctatga agaatggttg
    14761 ccgtcttata aaaatcgtgt acaagaaacg acttatcaac gaacaaaaga cctttttcgc
    14821 ttgcatatcc tgccaaagtt cggagacaaa tcgatttcta agattactcc tatttactgt
    14881 cagaatgcaa tcaatgaatg ggctgtaaaa tatacaaata tcaagcaact taaatcctac
    14941 agctctcaaa tatttgagta tgcaatcttc gcagaattgt tacagcgtaa ccctatgaca
    15001 aataccaaac tgccaaaaca agaaaagaaa gaatccgaaa attatttctc actagaagaa
    15061 ttaaaagagt ttttggaaat tctcaataaa gaagagtctt taaaacatat cctgatcttt
    15121 caacttttaa ttactaccgg tgttcgcaaa ggtgaattat cagccctacg ctgggcagat
    15181 attaattttg aagaacagtt actacacatc ggaaaaagtt atgcaacaat tcgcactgaa
    15241 gatccgaatg ctactagaaa aacaatcagg attcagaaga atacaaaaaa tgtctcttcc
    15301 gaacgaattt taccgattga tactcaaacc atcgaaatgc tgaaactttg gaaaaaagag
    15361 caagcagagg tacttttaca attcggcatt aatgcaaata ccaaaaagca acttatcttt
    15421 acctatgtaa atgcaaatgg cgatgttaac caacctttac acgcagatta ttccaataat
    15481 attatgagac gtttagagaa gaaatataaa tttaagcacg ttactattca tggattgcga
    15541 cacactcacg ccactttact tttagaaggc ggtgcgagca ttaaagaagc acaagatcga
    15601 ttgggtcata aaaatgctga aactacgctc aatacttatt cccatgtaac tgaaaaagcg
    15661 caacgaaatg cggtagacaa gtttattgct tacactggat tatagacaaa aaagttgccg
    15721 tcattacctt tttcattacc acaacgaaaa aaagcacctt ccaactgaat ggaaagtgct
    15781 tgaaatcgtt ggtataacaa cgattaacgc ttagagaatt gtgaagcttt acgagctttc
    15841 ttaagacctg gttttttacg ttcgaccata cgtgcgtcac gagtaagtaa gccagcacgt
    15901 ttaagagcta aacggaagtc aggatcaact tctagcaatg cacgagcgat accatgacgg
    15961 atagctcctg attgtccagc gtatcctccg ccgtttacgt taacaaatac gtcatatgcg
    16021 ccttttgttt cagtaacagc gaaaggttga ttgattactt cacgcaagtc agcatgtgga
    16081 atgtattctt caacatcttt tttatttaca gtaattttgc cagttcctgg tactagacgt
    16141 acgcgagcaa ctgcgttttt acgacggcct gtgccgatat attgaacttg tgccaatgaa
    16201 attccctcct attagattaa gtttgtgatg tctaaaactt ctggttgttg tgctgcatgt
    16261 ggatgttctg ctccaccgta tacgttcaat tttgtaaatt gtttgcggcc taaagtgttt
    16321 ttaggaagca taccttttac agaagtttcg atcaaacgac gagaattttt agcacgcaat
    16381 tcgccagcag tgattgattt caatcctcct gggtagttgc tgtgacggta ataaattttg
    16441 tcagtcgctt ttttacctgt taatttcact ttgtctgcat tgatgacgat gacgaaatcg
    16501 ccagtatcca aatgaggtgt gaaagttggt ttatttttac cacgtagtac agatgcaaca
    16561 actgttgaaa gacgtcccaa tggaacatca gttgcgtcta ctacatacca tttacgttct
    16621 acttcgcctg gtttggccat atatgttgta cgcacgttat tttcctccaa tttaagttct
    16681 gatgtttgac atacacttcg agtttccggg gctctttgtg gggcaaacaa taccatttaa
    16741 catattaccc gctaatctag tcaatgtcaa cgtttttctg cttgattgaa aaatttatat
    16801 gaaactttac ttcttttctt tcttttatat aaaagggtcg tttcctgtca aaatgaaaaa
    16861 caaaaagagg ctgtgacaaa agtcttgtca caacccctta ttttgaataa acggtgttct
    16921 cctgccaact cctcggtcac aagtcacaat atccataaaa cataagaagc tgttttttaa
    16981 aattgtttct tgtgactcgg agctgaacgg caggcttaca acctcttggt aaacggtgtt
    17041 caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgac
    17101 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt
    17161 tcaacaacag tttttacgcg tcgatctctg gcgctttgcc caaatgagct tggatcatcc
    17221 agatacgttt ttctgtggct gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc
    17281 cttcttctcc agaaacttcg attccttttt ggtaaagatc tgctagataa cgatagcctg
    17341 caacaagtgt ttccaaacgt tctggaatag tacgatccca gcggccaatt tcatcaggga
    17401 tttttgtgtt atctgcaaat tcacgcaatg tagaataagg tgaaccgtct aatgtgatca
    17461 agcgttcgga tacttcatct aactgagcgt tcaaatcatc catgaactca tccatcattg
    17521 ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg tgtttggtga acaacaacag
    17581 acatttggct tagatctgca actaattgat ttaatacttc tttggtttgt tggaatttca
    17641 tctttcttgc ctcctaacat tttcttacac ttaaaataat agtagacttt caatgaaaag
    17701 tacaacaaaa tcatttacct gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa
    17761 tcatttttca gaatcagcta attcttctcg cagttgagca aattgctgat aagtctgtgc
    17821 taaatgcttt tgtgtcgaaa caaataatgg atagattttc tgatattttt tatgatcagt
    17881 ttcttgtact ccaacttgct ctttcaattc tatcaattcc tgtgcttcac tgatactttg
    17941 gatctttccg atgcttctcc agcctaaaat cacagctcct aatgcggatg cttctgtgac
    18001 agtagggaaa acaagcgggt aacccagtac atcagccatc atctgacgaa aaatcggaga
    18061 ttgagcaaac ccgcctgtag cacggatctc tgtgagctga ttttcctggc aaactccttt
    18121 taaaatccga taaaggttga gacagatccc ttctagaatc gccttcatca tttctttttc
    18181 tgtatgttgc cgctgtaaac cgataaaact gccaaacgca tctgcgtccc aaagcggtgc
    18241 tcgctcacca agtaaatagg gatgaaaaat cagaccattg gcacccgctg gcacaaaagc
    18301 gatagccgaa agcagtgcat catatggatt ttctcctttt tctttagcag attgaacgac
    18361 ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc
    18421 tcccactacc caatgtttct gatccagaac ataacaaaac gtctccattt ctggatggaa
    18481 ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt cctacggtca aagcggcgat
    18541 cgtatcacca aaagcaccta accctagatt agaaagcggc ccgtctgccc ctcccaaaac
    18601 aaatgatata tcatttggca aacctagttc tgctgctgtt tttgatggta gacctgttac
    18661 ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt cgaatgttca agtagtcaag
    18721 cacttcttct gcccattgga attcatggat atcaaaatat ccagttcctg aagctgttcc
    18781 gtagtcgctg atcagttcgc caaacaatct ataaaagata tagtctttga taccgacaaa
    18841 ataagcagtt tttttgaata attcaggttg gctgttttgc agccacttga ttttgttcaa
    18901 tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa aattctttgc caagcacggt
    18961 atatttcagt ttttctgctt cgtccgctgc tcggttatct gcccaagtga tcattcgaga
    19021 taacggtgtt ttctcttcat ccaacaatat caggccatgc atcgcagagg agaaggagac
    19081 aaattgaatg attgtgtctg aaggcaactg tttaattact tcttttataa cggtgcagac
    19141 accttgaaag atcaattcct gatcttgttc tgccatccct tgtgtttctt ggatcaaagg
    19201 gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt
    19261 ttgtgtggtt ccgatatcta atccgattga aacgttcatt ttttcacctc aaattttatg
    19321 tatgtacaaa aatttagttt aagcttgcgt atcttggttt tgaaaagggg gagatggcgt
    19381 gttactttat tttatcattg gttgctgtat cggatcattt ttatgtttaa ccgcccagcg
    19441 gattccgtta ggtcactcta ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc
    19501 gctttcttgg tatgaattga ttccgattct ctcgatcatc gtccaacgat ttcgctgccg
    19561 ctattgcagg tgtcggttgc ccttctacta tttgttggca gaagccttat gtggtggtct
    19621 ttttgcctgg ttctttactt tttcttccgc aagaaatttt tcgacgttca tatggatgct
    19681 ttcggctttg ctattttcgt tgatggattt attttacttc atggtccatt ctcacacgct
    19741 attctgttct tgggcaattt tatggatttt ttggttgcag gccggcgtct tccaatggca
    19801 aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct
    19861 cggagctggc gatactctgt tgatgctttc ttggagtggt ggcttgtctc tagaagagtt
    19921 gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc gttttttctg tttactttat
    19981 ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct tttttgagca gtgcgttgct
    20041 gattgtcctt catcttaggt attgaaaaaa ggttgtgaca aacgacttgt cacaacctct
    20101 gatatatagt gttctcttac caaatccttg tgacttggag ctaggcgaca ggttcacaac
    20161 ctctccacta aatggtgttc aatcagctga ccctcagtat tagctcaaaa tgctggtttt
    20221 acaacctctg atatacggtg ttccataagt agctttttcg gcttcttatt tcttgacgcc
    20281 ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct cgataaagat tgtaatggag
    20341 cttgtttttc ttgtaataat cctgatggta atcttctgca ggataaaatt cgcttcttgg
    20401 ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga
    20461 acgctctgca atatttcgtt cttcttcatc attatagtag atcactggac gataagaatc
    20521 accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc
    20581 caccaactga tcataagaaa taacttctgg atcatattcg atctctactg cttctgtatg
    20641 ccctgttgtg cctgttgtta cttgttcata agttggattt ggtacatgcc cgccagtata
    20701 accggatgtc acagaaatga tccctggctg ctcttcaaat ggtttgacca tacaccaaaa
    20761 gcatccgcct gcgaaaactg ctttttttgt catataaaat cccccttttt tctcatcata
    20821 ccttgatttc caaaaggagg ataacgtttt gcttgtgaca gaaaaaacgg aaactattat
    20881 atacgtgata cggcttctga taaatgccaa aacgaacgta cttgatcaaa aacggttaaa
    20941 tcaggaatca catattcagc agaaggaaga tttatgtttt cacctattcc caatacatgc
    21001 atgttaccag ccaaagctgc ttgacaacca gcttctgaat cttcaatgac caagcatgca
    21061 tttggcggta cattcaattg ctgtgctcct ttgagaaaaa cttctggatc aggttttgct
    21121 tttgatactt gcgtgccgtc aataaggaca tcaaaataac ttgtcaaatt cgttttttct
    21181 aaaatgaggc gtgcattttt gcttgctgat cccaatccga tcttgatgtt tgtttttttc
    21241 aagtactcaa gaacagctat tgcaccaggc aaaacggatg tttcatccat tttttgaatc
    21301 gcttgaagat atagattgtt tttttgctca gcaaaggctg atttctcttt ttctgagaat
    21361 ttctgttcca tttttccaaa tgacagcaat cgctctagtg actcggttct ggatattcct
    21421 ttcagttgct cgttttcttt ctcagtgaat ggaatggaca gctggttagc taaaaccagc
    21481 cacgcttcgt aatgataacg agctgtgtct acaagaacgc catctaaatc gaatagtgct
    21541 gctttgattt tcatcgatta cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt
    21601 ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag
    21661 ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc tgctgctttt tcgcttcctt
    21721 taccaacact tccgcaaata gctatcacag gaacgccctc tggtactctt ctagcaatcc
    21781 caactggcgc ttttcctttt agactttgtc catctagacg tccttcgcct acgattacga
    21841 ggtctgcttg ctgtacccgt ttatcgaact ccagtaaatc caatacgtag tcgatgccag
    21901 agactatttt ccctgaagca aatgctaaca gtcctgcacc cattccacca ccagcactac
    21961 tgcccgatgc ctcgattact tgaggggcaa atctcatata aaaatcatgc atggcttggt
    22021 cagccactgc taattcattt tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc
    22081 cttcttttcc gcataaaaga ttagtaacat cagtaatgac agtgattttg gctttaatca
    22141 gctcaatagg cacgttcgtt ttatcaactg tagcaatttt acctagattt tctcccagtg
    22201 cttctacttt ttgattatct tgatcgtaaa aagcataacc tagaccttct gccatgccga
    22261 tgccgccatc gttggtacta cttccgccaa cgccgatgat gatgtgtcga acaccttgtt
    22321 gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga taacctaagt ggctgttgtt
    22381 ggtttagtgg gacatctttc aatccgacga tttctgccat ttcaaaaatc gcagtttgac
    22441 cgtctgaagc gtactggatt tctttgtttt tgccaaatgc attagtaacg actgctgttt
    22501 gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt tccttctcct ccatcgccta
    22561 taggtagcag atcatattca gcctgaacaa aaacttcttg aaaacctgat tgaatggctt
    22621 gtgctgcctc taaggctgtc agactttctt tgaaagaatc tggcgcaatc agaatacgca
    22681 ttattttccc ctccgttcca agatcagttc tttttccagc tggtattggt ctccattgac
    22741 aaaaataggt aatgtttctt tcgtctcttt ttttattact atttgttttt ttgtcaaaac
    22801 aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa attttttccc atgctgaagg
    22861 aagtcttgga gaaatagaaa gagttccatt cgttactgta atacctgaaa aaccaaatat
    22921 aactgctagc catagtgctc ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg
    22981 atctggtcct aaatcaatca ggcacgcttc ttggaataaa tgatacgccc attccatttc
    23041 ttggatacgg caagcctcta tggcgtgaat cgctttactc aacgatgagt cgtggattgt
    23101 tttgttttca tagtaatgca aattcttaga aacgattggt tgagaaaaat actcaggtaa
    23161 taagtataac agcatgacaa gatctgcttg cttgctgact tggagttcat ttacttcgct
    23221 acgggcataa tcaagtaaaa tagactggct gccaggacgt tctttgtaag tggtcaaatc
    23281 aataactggc ttactgagat acgtgtcatc ttgcggaatc agctgttctt tgttaggcac
    23341 tggcaaatat aacttatcta aaaaattttg acattctttc aaaaaaagcg gatcgccagc
    23401 ttgatattta acagctaagg ccacattata atgcgccaga tagttcgtat atgcattatt
    23461 gtcgatatat tctgtgtatt catctggacc aatgactctg tgtatctcta aatgatcatc
    23521 gacttttaca gctctgctca gccaaaaagc tgctgtttca tgaatgagcg tttttccctt
    23581 tgtttctaaa aacgattgat cctctgttgc ttggacatac tcttgaacgg cataagcaag
    23641 gtctgcactg atatgatgtt ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt
    23701 gatattcaat gcagcaaatt tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg
    23761 gaaaagtgca ccttgatagt gataatctct tgctttttgc cgtgcgcctt ctaatcgatc
    23821 ataacggtac agcagcaaat tctttgctac ttcaggaaac tgatataaaa agaaaggcag
    23881 gataaaaatt tccgtatccc agaaaacatg ccctttataa ccttcgccag ttagtccctt
    23941 cgcccctaca ctaacacttg gatcattgcg gggagtcata atctgtaaat ggtaagcagc
    24001 gaagtccatc gctaactgat cgaaagaaga atcgccttgg atcgttatcc gatgctgatg
    24061 ccaaaaatct gtccaataag acatcgactg ctctaaaatt tctgtataac taacgctttt
    24121 ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc tgaagagagg tagagagcac
    24181 gatgatcttc tccagcgtaa aggacgtatt ttttctgatc ttttctgaaa aggaagcggt
    24241 gatccggcga ttttttgctt ggaatacacc tgctttattg aatttagctg caatcccaat
    24301 atcaatttta ctttctgttg tctggtagga tgcactgatc aaatcgtcac caaatactcg
    24361 cagttctttt tcaaccaact gctgtgtacc aaaattggtt tgctgtccat cgatacccgt
    24421 tttcacatct acttggcagt cttcggtcaa cggcttgatg accactttca gggctgcctg
    24481 atggagatta ttttgatcag caacacgaca agtaatgact tccacttttt ttccttgagc
    24541 agtttcaatg agcgcatagc gtttaagctc tcccatttct aaatctagtg cccgctcgta
    24601 tgcaagaact ttcatcgtct caagagaaaa atgttcaccg tcgattttta tttcccatct
    24661 gatgacatct gggagattca ctaactcggc tgattctgct cctaaaggtc ggttatatac
    24721 accagctgca aacattcccc ttacttgatt ccgatagtct tcctcctggc ttcctctgat
    24781 accaaaaaat ccatttccaa tggtcatctg actcgcatac ttattgatat actctaaatc
    24841 aaactgtttt tcttctactt tcataaggta ataacttctc ctgtttctgc cgaacgatat
    24901 aaattttcca ccagattctg gatgacatat ccttgccagc catctgcaat catctgagtc
    24961 tggtcaccga gcgcccgatt cacaaaatga gccatactta gttcatgtgg gtcattaact
    25021 ggcgtggtct tttgtaagga aaccagcttt ccatctacat cttcgaatag ttcgcctgga
    25081 tacagattag cacctgcctg atcgccacaa aaagccacat tcagtttgtc ttctctgata
    25141 ttcaaagcaa acgaagtatt cagttggatg atcttgccgt tttctaattc cagtgtgcca
    25201 aaaattgcat cttctactgt gaatttgctt ggatcccatt tcccgaattt ccctgaattt
    25261 tttttcgtac caattttttg gaagctgtac gcccgaacgc tttttacttt aggaaatcca
    25321 gtaatataca tcgctgtatc aagcatgtga atgccataat cgatcaaagg cccgcctcct
    25381 tgaagttctt tgtcgataaa cgttccccag cctggaacac cagaccgacg aagcgcattc
    25441 gcttctacgt aataaatatc cccgagaaca tcaagatgat tcttgatcat tgttgcttcg
    25501 tcagaaaaac gatgctggaa tccataagcc aaaatctgcc cgtttttatc ggctgtctct
    25561 gccatctttc ttgcttgatc agctgtcata gctggtggtt tctcgcacat cactgaagca
    25621 ttagcttcga gggcagccaa tacatgttcg aagtgaaagc gattgggtgt acagacactc
    25681 acgatatcgg gagaaatctc attcagcatt tgtgtaactg atgtatacgc atgagaaaat
    25741 cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg gatcaaccac agcggccaat
    25801 tctaagacat ctgatctttt ttgataataa gcagcatgta ctttttctgc tacttgtcca
    25861 gcgcctatga ttgctacttt cttcatttat ttctccctcc tttgcttatt tacttattgt
    25921 tattctttct ctattctttc tctaaatcat ttaaatgacg cgctgtttcc agacagtttt
    25981 tcaagtattg caatgattcc tgataaattt tcacttcatt ttctcctcgc aaacgacatt
    26041 catataccat ataaccttca tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat
    26101 caattgcgcc agatcctggt tggtagcgat gattatctgc taaatggata tgacccacat
    26161 actctttttg cgtagtcaat gcttttgaaa tatcatcttc ttcaatattc atatgataaa
    26221 aatcagcgat gatctgcaca tgtttcaatt gattagagtc aatgtaatga cgcgcatccg
    26281 ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg ctccaaataa ataaccgtac
    26341 ctgtttcttc ggctacttta tctaaataac gaagtgattc gctgactgct ttatagtctc
    26401 cttctttgct tcggggtgaa accatcggcg gtaatcgata agtgaacatt ccccaagctg
    26461 ctgggataac gattcctttg cctccaacct cgcttagtgc acgcaaaatc tcagcaattt
    26521 gacgtacacc attaagacgc cgttcttcga taaagtcacc gatccaccca tcgtagccgt
    26581 tacaagctgt cactactggg atccctgttt ctttgattgc ctgtttgact gtgtcgatat
    26641 tttctattaa caacttaccg tcgatttcgt attcttcaaa tccacagtct ttgataaatc
    26701 taaatttttc taaaatgtct cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg
    26761 ttctcctcct cggacttgaa cgcccatttt gatactttct tctgggtgct ggtctacata
    26821 acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact ggatcaatga tttcttcaca
    26881 atccaagtaa ccgttcatca ataattccca acatgtgtcc tcgattcgtt tacggttcca
    26941 tcttgggtaa tctgggtttg gttcactaga tgctcgagca aagacgattt ttgcattatt
    27001 gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa ggtttagcaa aggcaacata
    27061 agaaatgatc ccgccatagc cgatccctct taaagaagct tgtaatgcgg cagcactgcc
    27121 actcgtttca ataactgaat ctactccacg tttatctgtc aattccttta gacgaaaacc
    27181 gacatctgct ttcataggat ctagcgtgta atcggcaccg tatttttctg ctaattctct
    27241 acgctgcgct atgggatcga tcccaacgat ggtagtagca cctgcttttt tagctaattg
    27301 aatcgcaatt tgcccgatag cgcctaaacc gaagattgct acagcatcac ctgctcgaac
    27361 atgtgcttcc cggataccac ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt
    27421 ccattgatcc aaccgatcaa gtttacgtag tttataatta tcaacaccgt ttgtaatcag
    27481 cgtttcacga atgggtccat aagtgcagac taactctcct ttttgatact ctgtcaccaa
    27541 actgcccgtt tcttcgatcg taccgacaat catatttcct aattgaaatt cgccaaatac
    27601 cacaccgcgt tggcttcctt cttctcttgg caagaatagg cgccattctt ctgaaaattc
    27661 ttcatcgata aatggactta atcctctaaa gtcgacaatt tctgttcctg gttttggagt
    27721 agcgaaaatt actcggatac gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt
    27781 gaatccggct attcttggtt ctaatgcaac taattttttc atcttttctt tcctctttct
    27841 tttcttttta tccttttacg ccgccttcga ctaaatcacc tttaataaat cgttcagata
    27901 acgcatacat aatgatgact ggtaaagcgg tcaccaatga agcggccatc attcgtcccc
    27961 aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat tggtaaagta aatttttcgg
    28021 aggtcgacag gaaaatcgaa gcaaataaat aatcattcca cgaaaccata aagcaataaa
    28081 cgaagacaga aatgattcca gaaatagcca atggtataat gatccgaata atgatctgga
    28141 tccggctaag accatcgatc attgctgctt cttcaatatc agtcggaatc gtgtcaaaat
    28201 aactttttag catgaatatc gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg
    28261 ctgtattggt atcatacaaa cctagaccgg aaatgatttt aaataatgga acaacgatca
    28321 aaataccgga aaacatataa acggtgtaaa agctagcatt gattttcgag cgagctttga
    28381 agcgtagttt tgacaaagcg tatgccccgc tgatcccaat cactactgca gttacggagg
    28441 ctatcaagga gatcactaaa ctatttttga aatacatgat gaatggaaaa atctcagcat
    28501 tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg
    28561 caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat
    28621 tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat tttcctgctc tttttatttt
    28681 tcatttcaag tccaccttct ttcgtgagag caagacgacc gctgcaataa tgatgaacaa
    28741 tagaatcgat atagctgctg cttttccaag atcattgaac gcaaaggctg tctgatacaa
    28801 ataaacgcct aagatattga ctttattcgt cagtagatag acatctgtaa acatatagaa
    28861 catccagata gatcgtaaaa caatcacagt tgctagtact ggtttgattg ccggcagtgt
    28921 cacgatcttg aacttctgcc aagcatttgc accgtccatt tcagctgctt cataaagcgt
    28981 tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac gcatacggat agtatctcca
    29041 gatagcaaac aaaacgacca taaagaagct agcaactggc tggtcgaacc acagcggtgc
    29101 ttcgttgaaa agctgcaacc tatctactaa cagatcattg acgattccat aactattatt
    29161 gaacatatat ttccaagcaa aaatcaaaga aatagatggc gtaacataac ttaataaaat
    29221 cagtgaacgc accgttttcc taaatctgaa cttgcggtta agcaaaatag ctacggctaa
    29281 tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga
    29341 acgataaaat tcactatcac ttaaaatttc aagataattg cttaatccga taaaaacggc
    29401 gtctgagtta ggattcagcg gcatatcgaa aaaactgatc tgaatattgg aaagcattgg
    29461 aatcagcact aaaacagcta ataaaagcat gcttggtgct aaaagtccca tggctaattt
    29521 taagtcagaa tgtttccttg gacgattcat tgaaacctcc ccctgataat ctgccaatag
    29581 ctatctactt ttttcgaaca ccattggcag aacttcttgt tatttcgtat ttttttgtgc
    29641 gtcagctaaa gcttgatcga catcttcgtt tccgacagtt acttggttca ctgcttttcc
    29701 gatcacacct gagcttgtga tatctcccat tttcgtgaaa ttcttttccc cgactagtcc
    29761 aaagacttgt acttcatcaa aagcactagc aatctcagat ggcagctcgc caaaagactt
    29821 gatgacttca ttttctttgt aaccatccag ttcgactact tgtttattca ctggttgagc
    29881 accaccagga gacatcaaga cccacttttc catgtttttc ggttgagata agaaagctgt
    29941 gaatgcttca gcagcctctt tttgtttctc gtctagacca gaagaaatcg tcaatccaga
    30001 tactgtgcca tagacagctt gatctttttg tgttgggata gcaaaaccga tattttctga
    30061 gttccccgct tcataaacag aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt
    30121 tccattcatg aaagcatctt tgatttctgt cacatcatta gagcctggca tcgtgtattg
    30181 ggataagttt tgataaaacg acaatgcttc tctcatcttt tccgtatcga tcgttacctc
    30241 gccttcatca tccaaaacat ttgctccatt tgacaaagca aattgagaga acgcttgctc
    30301 actcattgtt ccttcagcag taggcatagc aattccatat tgtttattct ctttatctgt
    30361 gaaatgttga gcaattttta aaatatcatc ccaattttct ggttctgaaa aaccagcttc
    30421 tgataatttt tctttgtcat accaaatacc ttgaacccag ccgctaattg gtgcagcaat
    30481 atagcttttt ccatcttctg aacgaacaag gtttttggct ccgtcataat aattatcttc
    30541 tccagcttcc tcgataacgt tttggactgc cgtctgatca atcagttcat ctttatccat
    30601 cactttagca aaatcctgac tgacttccat tacttctggt aatttaccgg aacgagctaa
    30661 cgtaacgatt tttgtattga acgctgattc ttctaccggc acttgcttga ttttgatgtc
    30721 tggattttct ttttcaaaat cagcaaccag ttcattgatc acacttaatc gttcctgttc
    30781 gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat tcgttagagc tttgactttc
    30841 accacaagca gtaagaagaa atccagtagc tagcagacag gtagtaacaa taattttttt
    30901 catttttttc cacctcttat tttattattt tcagccacat atattcatac ggctggaata
    30961 tcaactttcg ttcttgactg caatggataa tttgtccaga aaacaggtct aggtaatctc
    31021 cttttggtaa aagataacct gctgattgat tggaaaaatt atggataact aagactttct
    31081 cttccttcgc ttcgcgaata aaactgataa aagaatctgc tgagtccagt aagtaaatca
    31141 aactattcgg atggaaggct ttttcttttt ttcgaataga tagcatctgg gtcaattcac
    31201 catatactgc tgttcttaat ggatctgtct gcaactcttt ctcaatatct gctaacaaaa
    31261 atttttgccg attgattttt cgattctctc ctgttttttc tacgcctttg taatcatttc
    31321 gacttcccag cacactttga atataaattg ccggcacgcc tggtaacgtt accaaaaatg
    31381 aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt atctgttgga gaacttaacg
    31441 catctagata cgtagcgttt atctcatatg gactggtcgt tccatctggg tttttcttgt
    31501 agtttactcg ggcaccctct ttcttcagct gttccactaa ttcaaggatc gttttttccg
    31561 gcagaatacc tcgtaccgga ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa
    31621 aagttgtttt tttcgatgcg aaatcgatgg tttttaacca ttctttcaac ttacttgtat
    31681 tacccgaacg aatcgcttga agggtcaaag gtggtagcga aaactgatag accatctgtg
    31741 cttcattctc gccattccca aaataactga tattatcttt gtgtggcaca tttgtctcag
    31801 tgatcaatac cgttcctgga gctgcttcat cagcgattgc acggaataat tggatgattt
    31861 tatgcgtttc tggcaggtgt atacaagaag ttcctagttc tttccataaa aatcccacag
    31921 cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa caaaagaata tcgatcattt
    31981 tcaataaaac ttctggattt ttaaaattca agtctacttg atcttcgcta aaagtcgtcc
    32041 atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg
    32101 gtctagtcac gctgcttgta tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg
    32161 tatcattcaa gtttaaatat tcctgaaacc aagtactttc ggcagacata tgattgcaga
    32221 caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc tatatcttcc catgtgccca
    32281 aacggtcgtt gattgcctga taatttacaa cggagaaacc atcatcagat gtatatggga
    32341 aaaatggcaa gaaatgtaca actctaaatg tatctgccaa atacttttga taaaatttct
    32401 taaatgtacg caaagttggt tgctgctctt caaaaaattg atccccataa gtaatcaaga
    32461 aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc tttcttctgc tgataaacac
    32521 tgactttttg ttcaatcata gaggcggttt tctcagacaa atctgagtcg cgataaatcg
    32581 cttgtaaaag ttttgtatac atcggattcc cctttctttt ttgggagcgt tcccaacgat
    32641 ttgatcatat tatggaaaac gcttactgtc aatagcattt ttttaaataa ttttgatata
    32701 cacaaaaata caaataagaa agcttatgta tcaataatta atagcttctt aaaaaaatat
    32761 ggtctctcat gatcatgcaa gctattccta aattttcaaa atcatagaca aaaaagaatg
    32821 tctattgtac tatggaatta ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga
    32881 aacctactat ttatgacatt gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag
    32941 tattaaataa ccagccaaat atctctgata aagcaaaagc aagtgtactt caagcaatca
    33001 aagaattgaa ttatgaaccg agcaaaattg caagaggact gtcaggaaat ggttttgacg
    33061 cgattctagc attatctcat cgtacttctc atacgaccta tggcaatcct tttttttcag
    33121 atgttattca atccatttct gccgtttctg aacaaaataa cttcgacttg attctacaaa
    33181 cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga
    33241 tccgaggaat tattatttta agctctacga tggatgaaac acttcttttt gaattggatc
    33301 aatacaatat tcctattgtg gtaatcggaa aagtcggaaa cgactacacg aacatctact
    33361 cagtggacac ggataatttc caggatagtt acgatttggt ggaaacactg atcaagaatg
    33421 gacaccgtca tatcggctgt ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc
    33481 tcaatggtta ccgtcaatgc ttatttgatc accacctaga tatccgtaat gaatggatta
    33541 tagacggcgg ctttcgctta gaagatagca tggcagcaat gaatgattta atgaagctac
    33601 gagaaagacc aactgcgatt ttttctaccg atgcattaaa atcattgagt atttacaaag
    33661 tggcagaaca atctggaaag cagatacctg atgatctttc gatcgcagga ttcagtgatc
    33721 ccttattgtc ctccctattt tcgccacagt tgactcggat agacattccg acaaatcagt
    33781 taggaatcaa agccacagaa cttttattcc aactaatcca ccaccaatac cctgaagaaa
    33841 aaagaatcat tctttcaacc gagattactc tcacagattc gatccaacaa caagaaatgt
    33901 agaacagaat tataaaagaa cagaaacact gaggataagt cagttactct gttctttttt
    33961 caatcactca tccctcgtta ccccctcccg gtattttagc gaatttttct tattttccat
    34021 ctgttttaga tagctgtgga taactataat gtttgtgtta aactattttt tccacaggga
    34081 agaaaaaact atttttaacc acgttgtgta taagttttta tagttcgttt ttttaaacac
    34141 atttaccctt atttcaatcc atgttatttg gggatactta tctctaaaac ataaatagaa
    34201 taacagacat gtctactggt tttgttttat tcgaggaatt attcaataat agctagcaaa
    34261 atattaaaaa taaccgctct atcttcccat caatttattt tgcattcaaa aaatgatcta
    34321 tctttacttt tgttgattga cttctcttgg aagatgatcg ttccgatacc ttaattggat
    34381 acgaaccgat gttgttaaga tcctttccca cttttcttct agttagacaa attattttag
    34441 acaaaccaaa aaagaggttg tgacaataat ttgtcacaac ctctttttta aaatatctat
    34501 tttgtttgat tgttcattgc gtcttctgca gccatttgcg cttcaatatc tgaaatactg
    34561 tagacatttt cgcttgctac accgacttct tttggttcca tgtcacgata acgttgcata
    34621 ccagtaccag ctgggatgat cttacctata ataacatttt ctttcaaacc aagaagagga
    34681 tctttcttac cgcggatcgc agcatccgtc aagacacgag ttgtttcttg gaatgatgca
    34741 gcagataaga aactatttgt ttctaatgat gcttttgtga tacctagtaa gacaggtcga
    34801 gaagttgcag gaacgcctcc tgagactagc gtattgtagt tttgatcttt aaagtctgcg
    34861 atgtctaata atgtacctgg taagatatct gtatcacctg gatccattac acgaacttta
    34921 cgcagcattt gacgaaccat tacttcgata tgcttgtcgc cgatttctac cccttgcata
    34981 cggtaaacac gttgtacttc acgaagcaag tagttttcaa cagataatac atcgcgaact
    35041 tgcaacaatt gttttggatc gattgatcct tctgtcaatg gtgcaccacg gtggatcata
    35101 tcgccttctg ctactttcat acgtgctgta taaggaacag tatacgtacg tgtatctgtt
    35161 tttcctttga ttgttacttc tttggtacgt gttgcaggat cttctgagat atcgatgaca
    35221 tcccctgtta cttctgtgat cactgcttgc cctttcggat tacgtgcttc aaagatttct
    35281 tggacacgag gaagaccttg agtgatatcg tctccggcaa ccccacctgt atggaatgta
    35341 cgcatcgtca actgagtacc aggttcaccg attgattgag cggcgatcgt accaactgct
    35401 tctccgactt caacgtcaga gccagttgca aggttgcgtc cgtaacaatg tttgcagaca
    35461 ccatgttttg tattacatgt gaagacagaa cggattgtca ctgtttcgac acctgcatca
    35521 acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat caattcattt
    35581 gtttcaggat gacgtacttc tttacgggtg taacgtccca ctaaacgatc ttctaatgat
    35641 tcgatgattt catttccctc gcggatggct tggatttcaa gacctcggtc agtaccgcaa
    35701 tcttcttcac ggatgattac atcttgggcc acgtcaacca gacgacgagt caagtaacct
    35761 gaatcggcag tcttcaaggc tgtatcggtc atccctttac gagccccgtg agttgagata
    35821 aacatttcca agacggatag tccttcacgg aagttcgaaa tgattggcaa ttccatgatt
    35881 cgaccattag gagcggccat caaaccacgc attccggcaa gctgtgtaaa gttggaaatg
    35941 ttaccacgag ctccagaatc agacatcatg aagattggat ttttcgcttc caaaccttcc
    36001 atcagttttt gttggatgct gtcttttgca gcattccaaa cagcaatgac acgttcataa
    36061 cgttcgtcat ctgtgatcag accacgacgg aattgttttg taatagtttc aacttgttta
    36121 tgggcatttt caatgatttc ttgtttttca ttcagtacac tgatgtcagc gatccctact
    36181 gtgataccgg catgtgtcga atgtttgtac cctaagtctt tcatacggtc aagcatctta
    36241 gaagtttctt ttactttgaa tcgtttgaag acttctgcga tgatgtttcc taagtttttc
    36301 ttcttgaacg gtaatactaa ttcttgctct ttgatatgtg caggaatatc agtaccagct
    36361 tcaacaaagt atttgtccgg tgtttggacg gtcaagttga agtctgttgg ttcattcaga
    36421 taagggaatt caggaggcat gatctcgttg aagatgatct taccaactgt tgtgatcatc
    36481 gtacgttctt tttgccattc agtaaatggt ttttcaccta atgagttagg gttcacaccg
    36541 atacgtgaat gtaaatgaac atatccgttg cgccatgcga taacggcttc gttttgatct
    36601 ctgaagacca ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct
    36661 agaaccatat cttgagaagg agtaacaact ggtttaccat ctttagggtt caagatgttt
    36721 tgagcagcta acatcaacat acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg
    36781 acagccattt ggtctccatc gaaatcggca ttataagctt cacatacaag tggatgcaaa
    36841 cggattgcgc gtccttgtac taaaactggt tcgaatgctt ggataccaag tcggtgaagc
    36901 gtaggtgccc ggttcagcaa tactgggtgt tccttgatta cgtcttctaa aacatcccat
    36961 acttcgtctt cttgacgttc gattttacgt ttcgcatttt tgatgtttga agctagttcg
    37021 cgttgaacaa gctcacgcat cacgaatggt ttgaataatt caatggccat ttcttttgga
    37081 agtccacatt ggtacatctt caagaaaggt ccaacaacga taacggaacg acctgagtag
    37141 tcgacacgtt tacctagcaa gttttgacgg aaacgacctt gtttcccttt caacatatga
    37201 gaaagagatt tcaatgggcg gttacctggt ccagtaactg gacgtccgcg acgaccgtta
    37261 tcgatcaatg catctactgc ttcttgcagc atacgttttt cattttgaac gatgattcca
    37321 ggtgcattca agtctaacaa acgtttcaaa cggttattac ggttgattac acgacggtaa
    37381 agatcattca agtcacttgt tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa
    37441 tctggtggaa taacagggat aacgtccata accatccagc ttggttgatt tcctgaagcg
    37501 cggaatgctt ctaaaatatc caaacggcgg attgcacgtg tacgtttttg tccttgtgca
    37561 gatttcaatt cttctttcaa ttcggctgct tctgcatcta gatcaacatt atcaagtaat
    37621 tgtttaatcg cttctgcacc catagcagct tggaattctt gtccatattg ttcacgtttt
    37681 tcgcgatatt cgcgttctgt caacaattgt tttttctcta aattcgtatt tcctggttcg
    37741 atgactacat atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct catgtctaat
    37801 acaagaccca tacgtgaagg aatcccttta aagtaccaga tatgtgaaac aggagctgct
    37861 aattcaatat gtcccatgcg ttcacggcga actttcgaac gagtaacttc cactccgcat
    37921 cgatcacaaa cgattccttt ataacggata cgtttgtatt ttccgcaggc acattcccag
    37981 tcttttgtag gaccaaaaat ccgctcacag aacaaccctt cgcgttcagg ttttaaggta
    38041 cgatagttaa tcgtctcagg tttttttact tcaccgtaag accagcttct gattttttcg
    38101 ggagaagcca aacctatttg catactttcg aatttattta catcgatcaa aaggggttcc
    38161 ctccatttca tttaatggca aaccgcaaga cggctgatct tcgaccgcct tgcagctcat
    38221 agaccggatt agtctaaatt atcttctgcg gtttcaaagt tttggatgac atcgtctttt
    38281 tcatcttcca cttgttcagc agctttcttt tctaattctt tggcagtttg ctgttcggca
    38341 aattttgtca aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt
    38401 tcgatctctg tttcttcgat atccaagaca cgcatgtcca gacctagtga ttgcaattct
    38461 ttcaccaata cgcggaatga ttccggtaca cctggttttg gaattggttc acccttgacg
    38521 attgcttcgt atgttttcac acgtccgact acgtcatctg atttgtatgt caagatttct
    38581 tgtaaagtat aagccgcacc gtaagcttcc agtgcccata cttccatttc accaaaacgt
    38641 tgtccaccaa attgagcttt acctcccaat ggttgttgtg taaccaatga gtaaggtccg
    38701 attgaacgtg catgcaattt gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca
    38761 ccgacagaca cacgtccatc aaatggttca cctgtacgtc cgtcgtacag aacagttttc
    38821 gcatcgctgg ccataccggc ttcacgaact gtttcccata cgtcttcatc acttgcacca
    38881 tcaaatactg gcgttgctac gtggatacct aactgacgtg cagccatacc caagtgtaat
    38941 tccaatactt gcccgatgtt catacgtgaa ggcaccccta aaggattcag catgatatcg
    39001 attggtgttc catcaggtag gaatggcata tcttcttccg gcataatacg ggatacaacc
    39061 cctttatttc cgtgacgacc ggccatttta tccccttcat ggattttacg tttttgaacg
    39121 atataaacac gaactaacat gtttacgcct ggtgacaatt catctccagc ttcacgagta
    39181 aagattttca catcgtgaac gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg
    39241 cggacttcac gagctttttc gccaaagatc gcatgcaata gacgttcttc tgcagataat
    39301 tctgttaccc cttttggcgt gactttccct acaagcaaat cgccatcttg gacttctgca
    39361 ccaatacgga tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg
    39421 atttcgcgtg tgatttcttc aggtcctaat tttgtatcgc gggcttctga ttcatattct
    39481 tcaatatgga tagaagtgta tacgtcatct tttaccaaac gacggctcat gatgatcgca
    39541 tcttcatagt tgtacccttc ccaagtcatg aaaccaacta aaacgttttg gcctagagcc
    39601 atttcgcctt gttccataga tggtccgtct gccagagtat cccctttttc gactttctca
    39661 cccaaatgaa cgattggacg ttggttgtag cttgttccgg agtttgaacg acggaatttt
    39721 gttacatcgt atttatctaa tgcaccgttg tcgcggcgta cgcggatttc agaagcatca
    39781 acgtattcaa cgacaccatc atgtttacat aacaaagcag ctcctgagtc atgcgctgat
    39841 ttgtattcca taccagtacc aacccatggt gcttgaggat tgatcaacgg cactgcttga
    39901 cgctgcatgt tggcacccat caaggcacgg ttggagtcat cgttttccaa gaaaggaata
    39961 catgctgtcg caacggcaac tacttgttta ggtgatacgt ccatgtagtc tactttatca
    40021 atagatactt ctaagttttc actttgagca cgagccataa caacgtcttg agcaaatgtt
    40081 ccatcttcat tcaatgggct gtttgcttgg gctacgatgt aatggtcttc gatatcagct
    40141 gtcaagtagt cgatttggtc agtaacacga cctgtttcac ggtctacacg acgataaggt
    40201 gtttcgataa atccgaattt atttactttc gcataactgg acaaactatt gatcaacccg
    40261 atgtttgggc cttcaggagt ttcgatcgga cacatacggc catagtggga gtagtgaacg
    40321 tcacgaactt cataaccggc gcggtcacga gtcaatccgc caggtcctaa ggcgaaaaga
    40381 cgacgtttgt gtgtcaattc gccaagaggg tttgtttgat ccatgaactg tgacaactga
    40441 gaagaaccaa agaattcttt gatacttgcc acaactggac ggatattgat caattgttga
    40501 ggtgtcaatg tttcagtatc ttggatcgac attctttcac gaaccacacg ttccatacgt
    40561 gctagcccga tacggaattg gttttgtaaa agttcaccga ctgaacggat acgacgattt
    40621 cctaagtggt cgatatcatc gacattgccg ataccttcca tcaagttcaa gaaatagctc
    40681 attgaagcaa tgatgtcggc tggacgaacc gtttttacag ctgcttctgg atagccgttt
    40741 ccgatcacgt ttacttcacg ttcagggtct cttggtgaaa atactttgat gacttggaca
    40801 gtcattggat ctgtgaccac accgtcttct gatggatagt aagtcactga atttaggcca
    40861 ttatcaatga aaggagctag tgtttccatg acttggtgag tcagaactgt tcctttttca
    40921 acgatgatct cgcctgtttc tggatcaact aatgtttcag ctaatgtcag attcaataga
    40981 cgtgttttca aatctaattt tttgtttact ttgtaacgac caacgtttgc taagtcgtaa
    41041 cgttttggat cgaagaaacg agcattcagc aagttacgtg agctgtctgc tgtttttggt
    41101 tcgcctggac gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt
    41161 gcgtttttat gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt
    41221 tcaaagatcg tatcatcgga accaaatcct aacgcacgaa ctaatacggt taaaggaatc
    41281 ttacgtgtac ggtcgatacg tacataagag atgtcttttg catcagtttc catttctagc
    41341 caagctccac ggttagggat aacagtagaa ccgaatcctt cttttccgtt tttgtcgact
    41401 tttccatgga aatagacacc tggtgaacgg actaactgag aaacgattac tcgctctgcc
    41461 ccgttgatga tgaatgttcc ttgttctgtc attaatggga aatcaccgaa gaatacttct
    41521 tgtgctttga tttcgccagt ctcgcggttt gtcaaacgca atgttacatg taatggtgca
    41581 gagtagtttg catcatgcgc acgagcttct gctactgtat actttggttc tttcaattca
    41641 taatctacaa attccaaaga tagattgcca ttgaagtcat cgattggcaa aatgtcttca
    41701 aacatttctc taagtccctc atctaagaac cattggtaag agtctgtttg aatttcgatc
    41761 aaatttggta attccaacac ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt
    41821 ccgtatttta ctacgtgtcc agccaagctc ttcacccctc taaaatgtca ttcaaaaaga
    41881 ctacccgatc aattgttaca ataatatttc aaatattatt tttaggtaac aacagacatt
    41941 ttttgcggct tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa accttacttc
    42001 gtgagcttct acttttgttt ccttatatta aagccgaaac ggacaatatt tcgtttctgc
    42061 cttttcttca tcagatagtt tttgcaactt catatactac acttattttc tataaaagtc
    42121 aacacttcct ggcgtttctt ctgctttttc gcaacatttt cttaatgttt tcaattttat
    42181 gttttcaatt atctgacaat tcttaattga cagacctttt tatctcggtt atactttaga
    42241 aaattcattt tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg caccaacaca
    42301 actgaataac ttttacttta gctactttag ataggtttgt attcatcatg atggatacaa
    42361 acgttaaacg acagtttgta tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc
    42421 acgtagacaa aatgctaagt ggctgagcag tgaaaataca aagtaaaagt atcaagcatt
    42481 ctactttttc aatttatttt ccgacttttc aatccatttg gacattttgt ctgaatggat
    42541 tttttgtttc ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat catcggatta
    42601 ggcgctgccc tgctactagc tttaggaggt tgcgcacaaa aagacaattc cgcagaggca
    42661 tcagatggac aaaaaacaat tggcgtattg caacttgtac aacataactc gttagatgct
    42721 gcctataaag gattcaaaga aggattagca gaagaagggt ataaagaagg cgataatcta
    42781 acaatcgatt atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa
    42841 ctcgtcaaag aagatcctga tttgctactt ggaatcgcca ctccagcagc acaatcttta
    42901 ttaaatgaaa caaccactat cccgatgact gtcacagcgg tcacagactt ggaacaagcc
    42961 catttagtca catccaatga aaaacctggc ggcaatgtga ctggcacgac agatatggtg
    43021 ccaatcgaaa aacaaatcgg cttactgtta aatatcgtgc cagacgccaa aacaatcggc
    43081 atcatgtata acaacggtga agcaaactct aagatccaag ctgatttggc agaaaaagaa
    43141 ttgaaaaaag cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa tgatgtccaa
    43201 caagtgacga aaagtctagc taaagatgta gatggaatct atattcctac tgataacacg
    43261 ttcgcaagtg cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt
    43321 gctggcgcag ttgatcaagt caaagacggc ggtctagcaa cagtaggaat cgactacgaa
    43381 agcttgggca aacaaacagg gatcatggca gcaaaaatct tagacggcaa gaatccaagt
    43441 gaacttcctg tcgaaacagc gaatgatttg gaactgtatg tcaataaaga catggccaaa
    43501 gcattaggca tcaatccaga cagcatcaaa gaagctgaat aaaacacagg aggattctca
    43561 tgacaaattt gctctctctt ttcttatcat ctgcatcgca aggggtctta tgggccctac
    43621 ttgcgattgg tgtatacgtc actttccgta tcttggacat tgccgacttg actgctgaag
    43681 gaagctttcc tttaggtgca gggatcgcgg cagtaagtat tacaaatggc tatcatccat
    43741 tgacagcttg tcttctaggc tttttaggga gtgcggcagc agggcttgtt tccggactgc
    43801 ttcacacaaa attgaaaatt cccgcccttc ttgcagggat catcaccatg actggtttat
    43861 attctgtcac atcccgtgtg atgggtgcgc cgaacatctc tttattgggt cagccaacga
    43921 tctttcattg ggtcgagtca ttcggcataa caaaagacaa cgctgtactg atcgtcggtc
    43981 tgctttttgc tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca gaaacaggac
    44041 ttgctattcg tgcaactggc gataatctgg caatgagcga agcaaacggg atcaacacag
    44101 ataatatgaa gatcatcggt tatatgattt ctaatggatg cattgctctt tctggctcct
    44161 tgcttgcgca aaacaacggc tttgctgatt taaattctgg tattggaacg atcgtaattg
    44221 gacttgcttc tattatcatc gcagaagttt tgttacgtaa ccagccacta tttctccgtc
    44281 tcttgactgt cgttttaggt gcggtcattt atcgttttat tttagcgtta gtcttcgaac
    44341 tgaatgttga accaagtgat tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt
    44401 ctttcccgca gatccagcaa aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca
    44461 aatgaatgac gtcttaacat tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa
    44521 cgaaaatcat gtcctgaaag gcattaatct ttccttagat cccggcgact tcgtgacgat
    44581 catcggtgga aatggggctg gtaaatctac tttgctgaac agcatcgccg gtacattttc
    44641 tgtcgatcag ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga
    44701 acgttcgaaa tccatcagtc gcgtattcca agatcctaaa ctagggacag ccgttcgttt
    44761 aacggtagaa gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg
    44821 tggtgtcaaa ccacaagacc gaacattttt caaagaccag ctggttcggc taaatttggg
    44881 gttggaaaat cgtttatcct cagaaatcgg tttgctgtct ggaggacaac gtcaagctat
    44941 tacgttattg atggccacat tacagcgtcc agaactgatc ttattagatg aacacactgc
    45001 agcgcttgac cctgctactt ccaaaacggt gatggaattg acagaaaagc tgatcactga
    45061 gcagaatctc actgctttta tggtcactca tgacatggaa gacgcgatcc gctatgggag
    45121 tcgactgatc atgctgcatc aaggaaggat cgtcgtagat atccagcaag aggaaaaaca
    45181 gcagctcaca gtgccccaat tattagcgtt gttcaaacaa aacagcggca cggcattaaa
    45241 agacgatcaa gtacttttat cttcataatc agaaagagga tgggacaaaa aatgaacgcc
    45301 caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca accgttgttt
    45361 cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa aggagcttga
    45421 tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag attttctttc
    45481 cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga aagccatcaa
    45541 agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc gttacctgcc
    45601 ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta ttcctgattt
    45661 gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac tggcagcagc
    45721 tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag ctcgtggacg
    45781 ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc tcttgctcca
    45841 tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca gtgcctgtgt
    45901 gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca atgatatcta
    45961 catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata tggaaagcgg
    46021 caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc aaaatcattt
    46081 tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag ctagtgtcac
    46141 aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt ctcacccaga
    46201 aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa
    46261 tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat
    46321 cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc tcacatctat
    46381 ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca gtgtcctttt
    46441 ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc tctatcctct
    46501 agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa atgaatcaat
    46561 gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc cgcttccttt
    46621 ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt gcgacttatt
    46681 ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa acgtctcgca
    46741 tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag acgcattcct
    46801 cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga aggttccagc
    46861 cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag cagatcttct
    46921 atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc ttgttcgtta
    46981 tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat tggagaagtt
    47041 cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc cagccaagtc
    47101 attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc ctcaatagag
    47161 acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc gcatagcata
    47221 tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt tcctaattct
    47281 aggataccta cttatatgtt actacacacc cttatttttt acactttaac agtcaccatc
    47341 atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc
    47401 aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca aacgaacagt
    47461 ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa
    47521 atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat cttttcgtat
    47581 ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat ttataatgat
    47641 ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt tgctaaaaat
    47701 aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact tgatgcaaaa
    47761 aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa ttatatacaa
    47821 aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag aatgaataaa
    47881 tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac agtcaatgga
    47941 tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg gcatgaatat
    48001 attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct tgcaacgatc
    48061 actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg gacaatcgat
    48121 ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga agtgttaacg
    48181 atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt taatgtcatc
    48241 aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat
    48301 gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga gatgactgat
    48361 ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac tatccgcatt
    48421 actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc agctaccaat
    48481 cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatactac agacacaagc
    48541 gacagtacag atacatctac cacaagtgat agctcagatt catcaacttc aagcgacagt
    48601 acagatacta cctctaccac aagtgatagc acggatacat cagcttcaag cgacagtaca
    48661 gatactacct ctaccacaag tgatagcacg gacacatcag ctt
//
LOCUS       DAEGIQ010000010        47467 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000010 DAEGIQ010000000
VERSION     DAEGIQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.010"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(52..786)
                     /locus_tag="KMT45_000503"
     CDS             complement(52..786)
                     /locus_tag="KMT45_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBE7003990.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1113..1523
                     /locus_tag="KMT45_000504"
     CDS             1113..1523
                     /locus_tag="KMT45_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBE7003991.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1516..2217
                     /locus_tag="KMT45_000505"
     CDS             1516..2217
                     /locus_tag="KMT45_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBE7003992.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2217..3803
                     /locus_tag="KMT45_000506"
     CDS             2217..3803
                     /locus_tag="KMT45_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7003993.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3822..4043
                     /locus_tag="KMT45_000507"
     CDS             3822..4043
                     /locus_tag="KMT45_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBE7003994.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4040..4801
                     /locus_tag="KMT45_000508"
     CDS             4040..4801
                     /locus_tag="KMT45_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBE7003995.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5181..5690
                     /locus_tag="KMT45_000509"
     CDS             5181..5690
                     /locus_tag="KMT45_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBE7003996.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5765..6304)
                     /locus_tag="KMT45_000510"
     CDS             complement(5765..6304)
                     /locus_tag="KMT45_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBE7003997.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6444..7055)
                     /gene="rpsD"
                     /locus_tag="KMT45_000511"
     CDS             complement(6444..7055)
                     /gene="rpsD"
                     /locus_tag="KMT45_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBE7003998.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7317..8168
                     /locus_tag="KMT45_000512"
     CDS             7317..8168
                     /locus_tag="KMT45_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBE7003999.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8196..8831
                     /locus_tag="KMT45_000513"
     CDS             8196..8831
                     /locus_tag="KMT45_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBE7004000.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8851..9624
                     /locus_tag="KMT45_000514"
     CDS             8851..9624
                     /locus_tag="KMT45_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBE7004001.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10032..10829
                     /locus_tag="KMT45_000515"
     CDS             10032..10829
                     /locus_tag="KMT45_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBE7004002.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10807..11220
                     /locus_tag="KMT45_000516"
     CDS             10807..11220
                     /locus_tag="KMT45_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBE7004003.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11204..13849
                     /gene="mutS"
                     /locus_tag="KMT45_000517"
     CDS             11204..13849
                     /gene="mutS"
                     /locus_tag="KMT45_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBE7004004.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISSVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13867..15975
                     /gene="mutL"
                     /locus_tag="KMT45_000518"
     CDS             13867..15975
                     /gene="mutL"
                     /locus_tag="KMT45_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBE7004005.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15997..16557
                     /gene="maf"
                     /locus_tag="KMT45_000519"
     CDS             15997..16557
                     /gene="maf"
                     /locus_tag="KMT45_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBE7004006.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16642..18630)
                     /gene="treP"
                     /locus_tag="KMT45_000520"
     CDS             complement(16642..18630)
                     /gene="treP"
                     /locus_tag="KMT45_000520"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBE7004007.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18853..19566
                     /gene="treR"
                     /locus_tag="KMT45_000521"
     CDS             18853..19566
                     /gene="treR"
                     /locus_tag="KMT45_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBE7004008.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19643..20089
                     /gene="msrB"
                     /locus_tag="KMT45_000522"
     CDS             19643..20089
                     /gene="msrB"
                     /locus_tag="KMT45_000522"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBE7004009.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATEQPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20259..20861
                     /gene="ruvA"
                     /locus_tag="KMT45_000523"
     CDS             20259..20861
                     /gene="ruvA"
                     /locus_tag="KMT45_000523"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBE7004010.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNEKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20874..21875
                     /gene="ruvB"
                     /locus_tag="KMT45_000524"
     CDS             20874..21875
                     /gene="ruvB"
                     /locus_tag="KMT45_000524"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBE7004011.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21904..22482
                     /locus_tag="KMT45_000525"
     CDS             21904..22482
                     /locus_tag="KMT45_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE7004012.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22534..23016
                     /locus_tag="KMT45_000526"
     CDS             22534..23016
                     /locus_tag="KMT45_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBE7004013.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23133..23507
                     /locus_tag="KMT45_000527"
     CDS             23133..23507
                     /locus_tag="KMT45_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004014.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24185..25537)
                     /gene="brnQ"
                     /locus_tag="KMT45_000528"
     CDS             complement(24185..25537)
                     /gene="brnQ"
                     /locus_tag="KMT45_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBE7004015.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25684..26274
                     /locus_tag="KMT45_000529"
     CDS             25684..26274
                     /locus_tag="KMT45_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBE7004016.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26402..27751)
                     /locus_tag="KMT45_000530"
     CDS             complement(26402..27751)
                     /locus_tag="KMT45_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7004017.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27919..28659)
                     /locus_tag="KMT45_000531"
     CDS             complement(27919..28659)
                     /locus_tag="KMT45_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBE7004018.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28672..30264)
                     /locus_tag="KMT45_000532"
     CDS             complement(28672..30264)
                     /locus_tag="KMT45_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBE7004019.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30832..31128)
                     /locus_tag="KMT45_000533"
     CDS             complement(30832..31128)
                     /locus_tag="KMT45_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004020.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31304..32029
                     /locus_tag="KMT45_000534"
     CDS             31304..32029
                     /locus_tag="KMT45_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBE7004021.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32022..32864
                     /locus_tag="KMT45_000535"
     CDS             32022..32864
                     /locus_tag="KMT45_000535"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBE7004022.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32867..33388
                     /locus_tag="KMT45_000536"
     CDS             32867..33388
                     /locus_tag="KMT45_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBE7004023.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33641..34204)
                     /locus_tag="KMT45_000537"
     CDS             complement(33641..34204)
                     /locus_tag="KMT45_000537"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBE7004024.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34455..34724
                     /gene="rpsO"
                     /locus_tag="KMT45_000538"
     CDS             34455..34724
                     /gene="rpsO"
                     /locus_tag="KMT45_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBE7004025.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34912..37038
                     /gene="pnp"
                     /locus_tag="KMT45_000539"
     CDS             34912..37038
                     /gene="pnp"
                     /locus_tag="KMT45_000539"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBE7004026.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37548..37814
                     /locus_tag="KMT45_000540"
     CDS             37548..37814
                     /locus_tag="KMT45_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBE7004027.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37991..39949
                     /locus_tag="KMT45_000541"
     CDS             37991..39949
                     /locus_tag="KMT45_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBE7004028.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40303..41604
                     /locus_tag="KMT45_000542"
     CDS             40303..41604
                     /locus_tag="KMT45_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBE7004029.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41734..43104)
                     /locus_tag="KMT45_000543"
     CDS             complement(41734..43104)
                     /locus_tag="KMT45_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBE7004030.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43286..43609)
                     /locus_tag="KMT45_000544"
     CDS             complement(43286..43609)
                     /locus_tag="KMT45_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBE7004031.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43934..44542)
                     /locus_tag="KMT45_000545"
     CDS             complement(43934..44542)
                     /locus_tag="KMT45_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBE7004032.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44872..45588
                     /locus_tag="KMT45_000546"
     CDS             44872..45588
                     /locus_tag="KMT45_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBE7004033.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45601..46296
                     /locus_tag="KMT45_000547"
     CDS             45601..46296
                     /locus_tag="KMT45_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBE7004034.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46380..47009
                     /gene="udk"
                     /locus_tag="KMT45_000548"
     CDS             46380..47009
                     /gene="udk"
                     /locus_tag="KMT45_000548"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBE7004035.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15390 a         8788 c         9877 g        13412 t
ORIGIN      
        1 aaaaaacttg gtacgcaatc gctgtaccaa gtttttttat ttagcttttg attaagccat
       61 aaataataat ttatataaga atactgctac gacaccagca cagataggtg ctacaactgg
      121 tacccatgca tatccccatt gagaagaacc tttatgtttc aatggaagaa gtgcgtgtac
      181 aagacgtgga gcaaagtcgc gggctgggtt tagtccagga cctgtaggac caccaagtga
      241 agcaactaat gtccatacaa ggaaaccaag tgctaagtga gctgtaccaa gattatcttg
      301 gaagaatggt gcttttgtaa tacctagggc accaaagaat aaaacgaatg aaccaaagaa
      361 ttcattgacg aaaccattga atttagaacc aacgctgttg attgttgaga atgtacctaa
      421 cacatggttt acgtcttctg tttggtcgta gtaaggtttg taacaagcta ctacgatcaa
      481 ttgtccaatc gctgcaccaa tcaattgtgc aatgatataa ggaacaactt cagcccaagg
      541 gaatagacca ttagatgcta aagcaattgt aaatgctggg ttgatgtggt taccgctgat
      601 tccaccaaaa atcattgctg gcatcataac cccgaatcca tacccgacag caatcagcat
      661 ccagtcactg ccatatcctt ttgtaccttt taaatctacg tttgctacag caccgttacc
      721 tagaacgatt agtaatgctg taccaatcat ttccgccgca agacgggtca ttagtgagta
      781 atccataaat acctccaaaa gttataaaaa tgcaaaaaaa aaagaaacat caacacgaaa
      841 agcatcggtg cagtgtttcg tgtcggaagg atttctgcag tcttaacaaa tgtgaataaa
      901 aaaatgaatt ccattactta taagaaacca gaccaacagt aaaactccaa ctctttttaa
      961 aagttctcca caaatcacca agcttaacta taaatatacc aaaaaaaaaa cgtttttttt
     1021 ctcaaaaatt gtatcttaga aacagcatgt tttgaaactt tcagaaaagg tttgttagta
     1081 aaatgaaagc tatacgtaag gagtgttgtt tgatgaaatt ttttcaagag tttttcatta
     1141 ttattttctt ttctgtatta ggagaaggca tacggatttt tgctcattta cctattcctg
     1201 gaagtatctt aggtattctt tttttatttt tagcatttga aatgcatctt gttgatcctg
     1261 aaaaaatcgg agcaactggt gattttttat tgaataatct agccatttta tttgttcctg
     1321 ctggtgtagg tttattggaa tattttgatg atattgcttc gatatggcct gttttactgg
     1381 gagcagtcgt cgtgtgttcc atcgtcacga tggttgcagc gggaaaaacg gcagaaggtg
     1441 tagaagcttt gctgggctat gtccgtaaga aaaaagcaga aagggtatct gtaaacgaaa
     1501 tggaggagaa tcaaattgac tgagtttttt gtgaatccat tatttggctt aagtttgact
     1561 ttaggagtgt acgtattatc tgacaaattt ttgcggaaat ataaactgcc tgttttgaat
     1621 cctctcgtat tatcgattgt tgttattatc ttatttttgc atttttctgg gatcagctat
     1681 aaagactata atatcggggg caattttttg actatgctga ttactcctgc gacagtggca
     1741 cttgccatcc ctctttataa aaacttccgt ttactgaaag agaattttta tcctgtaatg
     1801 gctgcgattt tagcaggaat agtcgcaaat tgcttggtta gtgtagggtt cggttacttc
     1861 ttctctcttc ataaagagat ggtgatctca ttactaccta aatctgtaac gacggctatt
     1921 tctgtcgatt tatctcatac gatgggcggg gtgaatgccg tgacacttgc tattgttgtc
     1981 tctacaggta tttttggttc gctgatcgcc actcatattt tccgcctatt caagatcgaa
     2041 agtccagttg ctcgcggtgt ggcgttagga agtacttctc atgctattgg tacagcaaaa
     2101 gcaatcgaga ttggtgaaat agaaggaatc atttccggac ttgcgatatg cgtcaacgga
     2161 atattgaccg ttttattgct accgttattc tttcagcctt ttgctggctt gttttgatgc
     2221 gaaaaccact attttatgct acaataacca aaacgttcga gcataaaaaa ggagggaaag
     2281 acgtgtattt gtcttccgag tatcttaaac aatttgataa agatctatat tataagattc
     2341 tcctcctaga aagttttgag gatcaagctt ggcataccgc tgcacaatta gcgcaagttg
     2401 ttcagctgga tgcccgcagc gtgtcgaaat acctgaacga gctgtctaaa aactatcagc
     2461 agtttagcgg taagacacac cctttgttta caaaaaatca ccgttctggt tataactttt
     2521 atgatacgct cgattcaatt gaacatgagc gttttttgat ctatctcgtg caaagtacgc
     2581 tgaaattcca gttgctgcac gacatttttt ttgaagaatt ccatacaatg taccaatttg
     2641 cgcaaaagca ttacattagt gaatctactg ctcatcgaaa aatcaatgaa tggaaacagc
     2701 aactgcaaac ttacggaatt cggctgcaac gaggtacata tatcgctcaa ggcgaagaag
     2761 aaatcattcg actttacttg catatgacat tctggcagtt gtttcgaggg aaaatctggc
     2821 catttgaaac gatttctcaa atggacgtaa agaatatggc agaacatatc atggcttttt
     2881 ttaatgttcg tctgaacgag atcaaaaaaa gacgattgga atatatgcta ggtgcctttt
     2941 ttttaagaaa atcacagaag cattatgttg tattgaatga gaaaaaaaga cggctgattt
     3001 ctgacaatct gttgttccag cgattttgcc aggtcatgga gccggtattt cctaattatt
     3061 ttcaagtaga agacgagtta ggcgcattat ttcttgtact gatgactcgt gaagaatatt
     3121 atagtgatcc taagatacgg aagaaaattt ttgattttca tcaacaagca aaaacaccgc
     3181 cctttacagc cctttcagaa gcaaaagccg ctctttctct ttatcaggaa gagcaggggc
     3241 tgccagcaga aaatctaacc ttcgaggcag aaaactattt attcagcagt cattttttcg
     3301 cctatctttt tcctaatgcg aaagaaacga ttgatggcaa tagtagtgat tttattaatc
     3361 atttagttat tgaaaacaag gaattgaaac aatggctcgt acattttttt gaaagtagac
     3421 acaaacatcc caatcatctg gcatttaaaa atcatgcgtt tttgatgggg cggtatctga
     3481 ctgtgtttaa aacattagga gcctttactc cacagcttcc aaagatcacg atccttttaa
     3541 tgacagattt cccattattt gaagagcaac tgttagagga gggattacgg acatttttcc
     3601 gtaatgagta tcagctgatt tttctgccaa cagattatcg ggggagagaa gttgatcttc
     3661 tgatttcgac aagtaaagtc caccgcaaac catgggcgga tcttgactat tttattgtga
     3721 ctgaagaact aaaattaatt gattacatcc aactatctca aaaatttgaa atgattcaaa
     3781 aacaaaaact attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt
     3841 taccagaatt gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt
     3901 atgttgttct cgtatccgat gatgaatttg atggctgtcc ggtgacgccg tacttattga
     3961 tcaaaacaag tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag
     4021 atgattgggc gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt
     4081 tatcacagga gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga
     4141 tgcttttgta tttggattag atcaaaagtt cggtaaaatg gaagaaattt ctttgcgatt
     4201 tcctgaaact tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc
     4261 ggtatcgaaa tgccaaaatc attttggtga agttacgatt ttactgaata cagcggggat
     4321 attggatgcc tacaaaccct tgatggaaac tgacgaatcg ctgtgggatc tgatttatga
     4381 gacgaatgtg aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgcca
     4441 aaaaagcgga acgatcatca atatggcttc gattgctggc ttgattgctg gtggaggtgg
     4501 gatcgcttat acaagcgcga aacatgcgat tgtcggattt accaaacaac tcgctctgga
     4561 ttacgcagga cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat
     4621 gaatgcggct gattttgaag ggaaaggtga gatggcagag tgggtggcaa atgaaacacc
     4681 agtcaagcga tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc
     4741 tcaagcaagc tacatacaag gaacgatcat cccaatcgac ggcggatggt tgttaaaata
     4801 accttgtgtt tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg
     4861 ataaaaaaag tcgatgactg ttcccgacat tcgatgccga acagccagtt caaaaaattc
     4921 tttttaataa tcgttaatat tcgaaactaa gcggcttttc acgacctctt gatataaacg
     4981 atattcaaaa gcttgcaact gcggtaacaa gtccagccaa atgaaaaata taaggagcta
     5041 tttttatttg accattctta ttacttgttg cagggcagct ttttcactac cttctgcgac
     5101 acttcagttg gaattttctt cccatccttt ggtcgcggtc attaccaaga taaaattatc
     5161 ttgcaaagga gaaatagaac atggaaaaaa ataatacccg ttcagccaaa cgctggacaa
     5221 cgacagatgt gactcggatg gcgctagtca ctggtttata tgtggcagtg acgctggttt
     5281 tgtctgtctt tagctttgga gtgattcaga ttcgtctctc ggagatgttc aactatctgt
     5341 ccttgtataa caaaagatac atcgtcgcgg taacagcggg agtagctttg gctaatattg
     5401 cttctcctct tggactgata gatgttgcag taggaagcgt cagcacgctg cttgtattgc
     5461 ttatcaacta taaaatcact agtcgcatca aaaacatgaa aatcaaaatg gccgtgacag
     5521 catgcgtatt tgctttttcc atgtttacag tagctggtca gctaacgatt ttatatcaac
     5581 tacctttttt cttgaattgg tgggtgattg ctttaggaga attggcatca atgattgtcg
     5641 gtgggataat tatttattgg atcggtaaaa aagtggattt gtcaaaataa agatctgttt
     5701 aatttaaaat agggcagcat ggaagagact gaatgttgag tcttcttcca tgctgctttt
     5761 ttgattaatt tgtatgaaga aatcgattta gccgtttttt catcaagata ccgatataag
     5821 ctgctccagt tgccttgatt gcttccggca gtaaaaacgg cagtagtcca ctggcaaatg
     5881 cagttgtaaa agtaagatca ccacttattt tcagccagat cgttccgcag atcaatgcag
     5941 caagagagcc cattagattg gcaaagattg catataagta atgagcatcc gttctttcga
     6001 tcatccaacc agttatccac gtttgtaaga gaaatccaat caaatatccg ccagttggcc
     6061 caaataaaac agctatacct ccagtcatcc ctgaaaatac aggaagacca atcaagccaa
     6121 gtaaaagata aactccaaca gatatcaatc ctagttttct tccgagaaaa gttgctgtta
     6181 atccaatagc aaatgtttgc cctgtcaaag gaaccaaacc aaaaggaatg gtcatctgag
     6241 aaaggacaga aataatcgcg gcaaattccg cagcaagtat caattctttc aatgttcggt
     6301 tcataacgcc ctcttttcgt taacttaata tttttatttg gttaacgaaa ttgtaaaaca
     6361 aagtgattac tttgtaaata gaaaatataa aaaaagacca gctatccaat agatagcggt
     6421 ctttttcaag aaagcttttg aaattataag ctttggttgt agtattcaac gacaagagct
     6481 tcgtcgattt ctggatatag ttcgtcacgt tctggtaaac gagtcaatga accttccaat
     6541 ttttcagcgt caaagcttac gaatgctggg cgtccaacag ttgcttctac agcttctttt
     6601 actgttacga tgttttgtga tttttcacga actgagatca cttgacccac ttctacgtgg
     6661 tatgaaggga tatcaacgcg tttgccatca actaaaatgt gaccatggtt aactaattga
     6721 cgtgcttgac gacgagtcgt tgcaagtcct aaacggtaaa caacattgtc taaacgttgt
     6781 tctagtaaga tcatgaagtt aacaccatgt ttaccttctt tgattttgct agcttttacg
     6841 aacaagttac ggaattgacg ttcgttcatt ccgtacatgt gacgaagttt ttgtttttca
     6901 gttaattgca tgccgtattc tgattgtttg ccacggctat taggtccatg ttgacctggt
     6961 gcgtaagggc gacgtgctaa ttcttttcct gtgcctgaca atgagatgcc taagcgacga
     7021 gaaattttcc atgatggtcc tgtataacga gacattaaaa tttcctccaa tgaatataag
     7081 tttttggagt aaaataatcc gttgaaaaat tcataatcat atagtttgtt cttcaatctt
     7141 cacctttgca gccgcggtta cacaatcgaa cccgagacgg ggcaacaaac tagggatgag
     7201 ttattatttt tctgctgcat tattttacac aaattacagt atatgataga tcatccacga
     7261 atgtcaagat aattagtaca taaaaacgct ttacaaacct aggctgtctt tataaaatga
     7321 gagggtatgt gcaaatggaa aaaagcgaag gaagtttaag gagaaaatca atgaatccgg
     7381 tatcaccatt atttgagaaa atcgatgctt caattgaaaa gaaggcgaat ctttttgaaa
     7441 acagcttttc acgttacgct gttcgagcgg ttctcgcctg tttgtttttg actttaggaa
     7501 cggctgtggc atttgcgatc gcgatgaagg gggaagatat cgcgcatggt ctaggaaaga
     7561 ttttatacgc atttatgttc agctggggtc tggtcatgat tctatacatg aacgccgaat
     7621 taggaacatc gaatatgctt tatatgaccg tgggtgtttt tcggcagaga cttgctttaa
     7681 gtaaggcagc caagattcta tttgcctgta tttttttcaa tatgattggc ggtatattat
     7741 gtggatttct aatttctatg actacgccat ttcaagattt aacgttggac aactttatgt
     7801 tagaatcaat agcagtaaaa ctagcaaaac cttcagcaac gattttggtg gaagggatgt
     7861 ttgcgaatat cgttgtcaat accgctgtac tgatcagcat gcggatgaaa gaggatgtcg
     7921 gaaaagtgat cacagtcgta ttcatcattt tcatttttgc ttttttagga tatgaacacg
     7981 tgatagcgaa ttttcctgcc tttacgctta cttactttgc ttctcacgga cagatggatg
     8041 gaatgacagt cagcaatgtg ttacataatt tgttttttgc tttggcagga aattatattg
     8101 gtggcggttt agtaatggga ctgggatatg cttggctgaa tcagtcaaaa tcatcctatg
     8161 tggactaaaa agtcgttcat taaaggggaa gtgccatgga agttttagtt tttccaaaaa
     8221 atgaggaaaa agcgttacaa atggaaaagt acatgaaaaa tcttttttcc tttgctggag
     8281 tacctaagcc ggaacgcgct cttttacaac aaccgttact ggatgagagc aaaaatttat
     8341 ctatttctga agttttagct tttgttcatt attattatga acaaatcgaa agagagtatc
     8401 agtatgtagc gatcgattta gtccagaaaa acatcaaacg gcttacattt gaagacttag
     8461 tcgtattgtt gccactagtt cagaaaaaag aatggtggga cagcatcgac tcttggagaa
     8521 aagtatacag cacatggtgt aaagcctacc cagagcagcg tgaagaagtc tttcaattgt
     8581 ttgaaaaaaa ggaagatttt tggctacgtc gaatagcaat cacgttgcaa ttaggttata
     8641 aacagcaaac agacacagga ctattgatcc gagcaataga cgaagatcgt caaacaaagg
     8701 aattctttat ccaaaaagca attggttggg cattacgcga ttatagtaag accgatgccg
     8761 attgggtaaa aagacagctg acaacagatt tgagtaagtt agctgttcga gaggcaagta
     8821 agtatttata agaaagcgag acgacaagag atgagacgca aattatttgc ttttgatatt
     8881 gatggaacgt tgttagattc aaacaaacaa gctttagata gcacaagaga agcattggaa
     8941 aaattacgca aagccggtca tctggtcacg attgctacag ggcggagccg atttcatgcg
     9001 caagagatca ttcgcgatct tgattttacg aactacattt tatgtaatgg agcggccggt
     9061 tttttagacc atgaacaagt atataagaat ttactagatc gcgaccagtt ggatgcgttc
     9121 gttcaagaag cgcatcatga gcagatcgat acagcttttg tcagcttaga caatatcaag
     9181 cgttcttctt ccaatgatgt aacggtgatg gaagaagcga tgcattcttt cggtgctgtc
     9241 ttgcctgaat tagatcagtt ttttgcagag gaacaagaaa tataccaagc gttagcattt
     9301 ttcgatcaaa catatgatgg gaagttctct tcatatgata aaattcgatt tgtccgctgg
     9361 catgagaaca gtgtagatgt cgtacctaaa aatggttcta aagcagcgac aattctgcac
     9421 ttagcagaac aagtaggcat ccctcatgaa gacatcatca gctttggcga tggacaaaac
     9481 gaccgtgaaa tgttgcgcat gtcaggaatt gggatagcta tggggaatgc tgtgccagaa
     9541 gtacaagcag aagctaagat ggtcactgat acaaacgatc aggatggaat ttggaaggca
     9601 ttgaaagagt taagcgtgat ttaaaagagg ttgtgaaaaa gctgttctgc atcaagtaat
     9661 aagcccagcc aaacaaaaat agctcctcct atttttcgtt tttctgggct tattaccgca
     9721 gatgcaagct ttcgaacacc gtttaaggaa gaggtcgtga cagaagtggt cagtttcaag
     9781 aaataagaag ggatttacga aaattgcttc tcaaattttt gtaaaattac ggcttatttc
     9841 tgaagaagta gatgaaacaa cgtttattcg ggaaatggga ctgtgacaag atgtttgtca
     9901 tagtcccatt tttttatttt atttttcaaa actaagaaat accaggaaag agttcctttt
     9961 gtttgacctt ttctttctta ttgaaaacaa ctataatata gagtgatgtt aaaagagaag
    10021 aggaatttag tgtgcgggta ttgtttattg gtgatgtagt aggttcaatg ggacgagaga
    10081 tgattactga atatcttcct agattgaaga agaagtaccg tccgcaagta acgattgtaa
    10141 atggtgagaa tgccgcttct ggacgtggaa tcacagaaaa aatctataaa aaatttttgc
    10201 aagacggcgt agatgtcgtg acgatgggga atcatacgtg ggacaaccgc ggaatctttg
    10261 agtttgtaaa tgaagctaaa aagatggttc gtccggcaaa ttttccggaa gggacgcctg
    10321 gacaagggat ggtctttgtc aaagtgaacc aagtcgaact ggcagtgatc aacatgcagg
    10381 ctcgttcttt tatggtggac ttggatgatc cttttcgtaa aatgaatgag ctggtagaag
    10441 aagccagaaa acgtacgccg atcatttttg tagattttca tggagaaaca acaagtgaaa
    10501 aacaagcaat gggctggttt ttagatggga aagtttcagc agtggtcggt acacatacac
    10561 acgttcagac aaatgatgcg cgaattttgc caagaggaac agcgtatctg acagacgttg
    10621 ggatgacagg tccctatgat ggtattttgg gtatgcgcag agaacctgta atcgaaaagt
    10681 ttctgactgc attaccaaaa cgttttgaag ttgtggaaca aggccgatcg attttaagcg
    10741 gctgtatctt ggaattggat gatacgacag gtcatgcaaa agaaatccag ctgattcaga
    10801 tcaacgatga tcgaccgttc atggagtaga aagagaggca gttttttgca gcaggagccg
    10861 gaaattcaaa aagaagtaag gaaactgaca aaacttttaa gagagaatga aacgatcatt
    10921 cgctacaaag aacttgaaga aaagatccag caaaaccagt atttagctga gttgagagaa
    10981 aaaatcaaac aagcgcaaaa agatgctgtt cattttgccc actatgacaa accagcagca
    11041 gaaaaagaag caatcaaaca agcggatcaa ttcatgcaag aatttgatca gcatccgctt
    11101 gttgtagctt atagaaaaca gctgttggaa gcagatgatc tgcttcatca tttaacaact
    11161 atgatacaag aagaaatcaa tggacaaata gaggaggaaa aacatgcctc aaaaaactaa
    11221 aaatactcct atgatggaac aatatctaag catcaaggcc caataccaag atgctttttt
    11281 gttctatcgt ttaggagact tttatgaact attttacgaa gatgcgatca aagcgtcaca
    11341 gcttttggaa ttaacgttga cgagcagaaa tcgaaatgca gaagaaccta ttcctatgtg
    11401 cggtgtacct caccatgcag cacaaggata tatcgatgcc ttgattgaaa aaggctataa
    11461 agtagcgatt tgcgagcagg tagaagatcc aaagacgact aaagggatgg tcaagcgcga
    11521 agtgatccag ctagtcactc ctggtacagt catggacagc aaaggattgt cggcaaaaga
    11581 caacaatttt ttaacagctg tagttagtca aggaaatgaa tttggctttg cttatgccga
    11641 tttaagtacg ggagaattga agacggctcg gttgcatgat gaagaagctg ttttaaatga
    11701 agcctctgca ttgcagacaa aagaactcgt attaggcagt gagatcactg atacccttcg
    11761 tgaacagcta agcgggcgtt tagggctagt cttttctcaa caaaatgaga tggaagaaaa
    11821 cgcggaattt agctttttga caagcgaact agtcgatccg ctggaaaaag aagcaaccgg
    11881 caaactattg acttatttat cggtgacaca aaagcggagt ttagctcata tccaaaaagc
    11941 agtagaatac caaccagatc attttttgaa aatggattat tattcaaaat tcaacctaga
    12001 attaagccaa tctattcgga ctgggaaaaa gcacggcact cttctatggt tgttagatga
    12061 aacaaaaaca gcaatgggtg gccgtttact aaaacaatgg ctagaccgtc cgcttatcca
    12121 ggcaaaacaa attaaagcaa gacaagaaat ggtcgcttct ttgctagaca gctattttga
    12181 acgtatcgat ttgcagtcag cattaacgaa agtctatgat ctggaacgcc tagcaggtcg
    12241 agtagctttc ggtaatgtca atgggcgaga cttgatccag ctgaaaactt ctttagaaca
    12301 agtgccaatg atcagagggt tgattgaagg aatcgatcaa ggtcaatggc aaagtctgct
    12361 ttcagaaatg cagccgatgg accatctcgt ccaattgatc gaccaagcta tccaagatga
    12421 tccgccgtta caaataacgg aaggaaacat catcaaagat ggattcaatg agcagctcga
    12481 tatgtaccgt tctgctatga gaaacggaaa gaaatggttg gcagaacttg aagcaaaaga
    12541 gcgtcaagaa acaggaatca agaacttaaa aatcggattt aaccgcgttt tcggttatta
    12601 tatagaaatt acaaaagcaa atcttggaaa tgcagaactg gagaagtatg aacgaaaaca
    12661 aactttagca aatgcagagc gtttcatcac accagaacta aaagaattgg agacacaaat
    12721 cctcgaagca gaagaaaaat ctgttgattt agaatatcag ttgtttttag ctgtgagaga
    12781 agaagtcaaa aaggctatcc agccgctaca agtacttgca aaagcaatca gttcagtaga
    12841 tgtgctacaa agttttgcaa cgatcagcga acgctatcag tatgttcggc cagaacttgt
    12901 cagtgacaag catcagcttc tgatcaaaga cggacgacat cctgtagtag aaaaagtatt
    12961 aggacatcag gaatatatcc ctaacagcgt agaaatggca gaagacgaaa tgatcttgct
    13021 gattacagga ccgaatatgt ctggtaaaag tacgtatatg cgacagttgg cattaactgt
    13081 tctgatggcg caaatgggct gtttcgttcc ggcaaaacaa gcagttttac ctatttttga
    13141 tcggattttt acccgaattg gtgcaagtga cgacttaatt gctggacaaa gtacttttat
    13201 ggtagagatg atggaagcca atcaagccct gcgttatgct acgccaaaca gtttgatttt
    13261 gtttgacgaa ctagggcgag gaacagcaac ctatgacgga atggcgttgg cccaagcaat
    13321 catcgaatat atccatcgtc atgtacaagc aaaaacactc ttttctactc attatcatga
    13381 actgacggta cttgagaaag aacttccgca gcttaaaaat gtccatgttg gagccgttga
    13441 aaaagacgga gaagttgtct tcttgcataa aatgatggat ggacctgcgg ataaaagcta
    13501 cggcatacat gtggcaaaga tcgcaggatt gccttccaat ctactagaac gagcagcaga
    13561 tatcctgcat acgctagaaa atggcgataa cggacaccat actgttccag ctgaagtaga
    13621 agagaaaact aaaacagcca aacaggagca aataaaacca cctgcagaaa aagtaaatca
    13681 agaccatata caagaccgtg taaaagaaga aaaccaacag ctttctttat tcagtgaatt
    13741 atccgaaaat gaaacagaag tcattgaatc actgaagcaa ataaatttac tagaaatgac
    13801 gccaatggat gcactaaaca cattatatga attacaaaaa caactgtagg aagaaggcgg
    13861 atatcgatgg ccaaaatcca agaattatct gaacgtttag ctaatcagat cgctgcggga
    13921 gaagtagtgg agcgaccagc ttccgttgtg aaagaattag tggaaaatgc aattgacgcc
    13981 ggcagtactc agattgatat cttagttgaa gaagcgggac tgcggacgat ccaagtgatt
    14041 gataacggag aaggcatttt ggcagacgac gtagagaatg ccttcaagcg gcatgcgaca
    14101 agtaagatcc ataatcgcga cgatcttttt cgcatacgaa cgctgggctt tcgaggggaa
    14161 gctttgccaa gtattgcctc tgtttctgaa atgactctgg aaacggcgaa tcaggaagaa
    14221 aaacaaggca cctatctgtc gctgaaaggc ggagaagtta ttgagcatcg accagcacca
    14281 ctgcgtcaag gaacgaagat taccgtcagc aatctgtttt tcaatacgcc agcccgcttg
    14341 aaatacgtga aaacactgca cacagaatta gcgaacatcg gggacatcgt caatcgactg
    14401 gcattgagtc atccatcgat tgcttttcgt ttagtacatg atggaaacaa aatgctggca
    14461 actgctggaa atggcgatct gaaacaaaca attgcgggaa tctacggatt atctaccgca
    14521 aaaaaaatgc tgaaaatcga aggaaaagat ctagattttg agttatttgg ctatgtttca
    14581 ttgcctgagg tgactcgtgc cagtaggaat tacttatcga cgatcatcaa tggacgtttt
    14641 atcaagaact tttccttgaa taaagcaatc gttgcaggtt atgggtcaaa attaatggtc
    14701 ggtcgtttcc ctattgctgt attggaaatc aaaatggacc cgcttttagt agatgtaaat
    14761 gttcatccga cgaagcagga agtccggctg tcgaaggaaa aagaactaac agagctaatt
    14821 agtaacgcca ttcaaaaagc attgtctcaa gaaaatctga ttccaagtgc agcggataat
    14881 ctacggttta agaaaaaagt acctgatcag cctaaaacag aacaaatcga aatcgatttg
    14941 tcctacgctt ttgaagaaga aaaacctaaa aagccaagta gtttgaattt tgatcgagag
    15001 acaggtacct ttttcgtaga agaactaaag aacacatttg ttgacagaat tgttgataag
    15061 tctgtggaaa atgttgaaaa ctattctggt ttttctgaag aaaacaccac ttatccagaa
    15121 aaaacagcga aaacggtgga taagtttggt gaaaatgttg ataactttgt agaaaaaaca
    15181 gtggaaaaat ctgaaaagaa agaatttccc attgtggata acatggagaa aacaaatgaa
    15241 gaacaagcag ataaatccgt agaagaggat gaaaagattt acgaggaaga attgacactc
    15301 catcctgaat ttgatttcac tcaaggaaac gacaaaacat tagaacaagc catcaagaaa
    15361 ttagaaacgg aaaaaccagt acaacgtttt cctcagttgg aatattttgg acagatgcac
    15421 ggaacgtatc tttttgccca aagtagcgat gggttgtaca tcattgatca gcatgctgcc
    15481 caagagcgca ttaagtatga atatttccgg gaaaaaattg gggaagtatc tgatgatctg
    15541 caagaattgc ttgttccatt tgtgttagac tatccaaaca gtgatgcttt gaaattaaaa
    15601 gagcaaaaac acttgctaga agatgcaggg atttatttag aagaatttgg tcaaaacagc
    15661 tttattgtac gggcccatcc tacttggtat cccaatggag aagaagaatc gattattcgg
    15721 gagatgatcg atatgctgct gacaactgga agtgtgagtg taaaaaaatt ccgtgaagca
    15781 acagcaatca tgatgagttg caaacgatcg attaaagcca accattattt gaatgaagca
    15841 caagcacgtg tgctgctgaa ggatctggca cgatgcgaaa atcctttcaa ttgtccccat
    15901 ggccgtccag tattgattca ctttaccaat tcggatatgg aaaggatgtt caaacgaatc
    15961 caagacccgc attaagtcaa agagaagagg gaaaacatga agattgtatt agcatcccaa
    16021 tcaccaagaa gaaaagagtt attggctcat atcgtttcag aattcgagat tgaacctgct
    16081 gatatcgatg aaacgccatt tccagacgaa cagcccgtcg attatgttcg tagaatggct
    16141 gaagaaaaag cacgaactgt ctgggaacaa gatgaaaaag aagaccattt agtgattgcc
    16201 agtgacacga ctgttgttct caatcaagag atcatgggta aaccagaaga tctggctgag
    16261 gctgaattca tgctgaaaaa actaagcggg gaaacacata ctgtatatac agctgttgtt
    16321 ttaaaaacaa aggaacgaga agaacggata ctagcagaag cacatgtcac tttttatcct
    16381 ttgacagacg aagagatcaa acgctacttg gagacggggg attatgcgga taaggcaggg
    16441 gcatacggca tccaaaatgc tgcgagtgta ttcgtcaaag aaatttctgg agattattac
    16501 agtatcgtag gatttccgat tggtgctgtc aatcaggcac tgaaaacatt catttaaaca
    16561 aaaaagcgga ctatctgatt ttcgattgaa aatccatgat agcccgtttt ttttattgaa
    16621 aagattactt atctactctg actaagattt tacttcagct ttaccgactg cttcgcccgg
    16681 gcgaacaagt cctgattggt caagagaaag tcctgcaact ttttcattat tggtcaaagc
    16741 aacaataatg tcagttttct tgccagcttg ttctatttct ggcaaatcca tttcagcaat
    16801 cagggtctct ggtgtgacaa ggtatccttc tttcacgaat acattgaaag gaacaccctt
    16861 tagttcaaca gtatctaatc ccatgtgaac aagtacttcc agcccctcat tagaaagaat
    16921 accaatggca tgttttgttt caaagacact cgtcacttta ccgtttactg gtgcatagac
    16981 tttgccgttt gacggttcga cagcataacc gtcacccatc attttttgtg agaaaactgg
    17041 gtcatttact tcagttattt ccttgatggt ccctgcagcg actgcgaata atgtttcttt
    17101 tgttgcaact gccgtaccag ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc
    17161 gccatttgct gtttgaagtt gtacatcagc agcattttca ggaacagcag gatcaatctt
    17221 attcaaaatt ccctgacgtc taaagatgat cgtcaagcca aatggaatga tgacagcaat
    17281 gatcattgcg atgatgaaag gtacccatgt ttctgcttgg atagctagta ttcctggcaa
    17341 accgcctact ccgatcgcgt tagctcgaac acccatcaag ttagcaaaca tgccagctaa
    17401 tccagagccg accattgcag cgacgaaagg atagacgtat ttcaaattga ttccgaacat
    17461 agcaggttca gttacgccta agtaacaaga aatcattgct ggaatcgaga tttgttcttc
    17521 ttttttattc ccacgatgta agaagatgat ggctaagact gcagagcctt gggcgatatt
    17581 tgataaagca atcatcggcc ataaattcgt tgatccgaaa tctgcaatca gctgcatatc
    17641 gatggcattg gtcatatggt ggagaccagt aatcactaaa ggagcataca ggaatccgaa
    17701 tacggcacta aacaaccagc tgatagaaga cgtcaatcca gtatttacga tatttgagat
    17761 ccagcttccg actgtccagc cgataggacc taaaataacg tgtgcagcta aaacagttgg
    17821 taacaatgaa aacaatggaa caaaaatcat cgagatcgac tgagggatat atttgcggaa
    17881 gaagatttct agatacgcta acataaaccc tgccagcatg gctggaataa cttgcgcttg
    17941 ataaccaatc atctggactt gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc
    18001 tgtggaagct acactataag catttaataa ttgcggagaa actaacgtaa ttcctaaaac
    18061 gattcctaat atttgtgtag tacccatctt ttttgcaata ctccaagtga tacctactgg
    18121 aagaaaatgg aaaattgctt cacccggtaa ccacaaaaag gcattgacac cattccaaaa
    18181 cttcgagtgt tcaacgattg ttccaccaag actttcaaac tggatacctt caagtacatt
    18241 tctgaatcca aggatcaatc caccgacaat gattgcagga atcagtggtg taaagatttc
    18301 tgctaaaacg gcaatagcac gttgaacggg atgaaggttt tgttttgcag ctgcttttcc
    18361 ttgttctttg gaaacccctt caactccaga tactgcaacg aagtcgttat aaaaagtaga
    18421 aacatcgttt cctatgatga cttggaactg tcctgcattc gtgaacatcc cttttacgct
    18481 aggtatatct tcgatcgctt tttcgtccgc ttttcctgga tcattcagga caaaacgcat
    18541 acgagtcgca caatgactaa ctgcagcaat attttctttt ccgccgatat catgtaacag
    18601 cttctcggca tctgcttgat attttgccat atcaataaac ccctttctct taacttgtct
    18661 atgcaagttg tttcttcatt tactatactt tttttcggag agaattgcaa gcgttttatt
    18721 aaaaagtacg agagaaaatg atatcgtttt aaaaagctta aaataagctt ttcttaaaaa
    18781 taggaacatt gaaatacaaa aagagctgta gtatagtgaa gaaatgatgg aagaacgagt
    18841 gagggaaaaa acatgaataa gttcaatgaa atttttttag atttggaaca gaaaatttta
    18901 gcgaaagaat atcctccgca ttcgctttta cccagtgaga accaattgat caaaatttat
    18961 ggtgtttcac gagaaacaat tagaaaagcg ctgaatcttt tgactacgga aggttatatt
    19021 caaaaaaaac aaggaaaagg ttcgatcgta ctggatcgga atcgcttcga ctttcctatt
    19081 tctggattga ctagctataa agagctacag gagacccaac gtattccaag tgaaacgatc
    19141 atccatacgt tagaagaaac agaagtgaca aaagcattga acgagatcac tggctgggag
    19201 atcggtgcgc ctgtttggca tttgatacgc gaaagaaaaa tcaatggcga agtagttata
    19261 ttagacaccg attatctatt aaaggaaatc gttcctattt taactccagc acaagcacaa
    19321 ggatcgatct atgaatattt tgaaaatgag ctttctttga cgattgctta cgcgcaaaag
    19381 gagatcacag tagaagaagt aacggaaatc atgaaaacag cgatggatct tcacgatgac
    19441 acttatgcag tagtagtcag aagcctcgtt catctggaag atacccgctg tttcgagtat
    19501 tctgagtcgg ttcaccgatt agataaattc cgtttcgttg agtttgcccg acgccgaaaa
    19561 gtgtaaatag ttcaaaagaa agcaaattgt tggcatctgt ccactttggc gctatgatag
    19621 gatcacaaag ggggcatttt taatggataa accaataaga ccagaaaaag aagaactgaa
    19681 agaaaagtta tcacccatgg cttatgcagt cacacaagaa aatgcaacag aacaaccatt
    19741 tagcgggaaa tatgatgatt tttacgaaaa ggggatctat gtagatatcg tcagcggtga
    19801 accgttattc agctctgcag aaaaatatga tgcaggttgt ggctggccat cattcagcaa
    19861 accaataacg aaaagacaag tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat
    19921 agaagttcga agcaaagaag cagattctca tctcggacat gtgtttaccg atggaccagt
    19981 agatcaaggt ggtctacgtt actgcatcaa ttctgcctca ctgaaattca ttccatatga
    20041 aaagatggat gaattaggtt atggggaatt caaatcactt gtagaataat ggaaatacac
    20101 aaagagcggt acaatacggg gatttactcg atcgtccgct ctttttttat tcaagcgatt
    20161 gctatttgaa agaaacgctc cctagactcc ttttttcaca aggtgaaatg tgctacaata
    20221 aagggaacgt atgtgtgcaa aagaaagagg actagaatat gtatgaatat ctgacaggtg
    20281 tggttacctt tatcaacccc tattacatag tagtagaaac caatggtatc ggctaccaga
    20341 tcgcactagg aaatccttac cgctacagta gcaaactaaa tgaaaaaata aaaatttatg
    20401 tccatcaggt aatacgtgaa gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa
    20461 agcaactgtt tttgcgatta gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa
    20521 tcatggcaag cgaagaccac tccggtttga ttcaagcagt agaatccagt gatgtcactt
    20581 atttgacgaa atttccagga gtagggaaaa agacagcaca acaaatgatc ttggatctaa
    20641 aaggaaaatt cggtgagctg agtatcgata caccttttag tctatttgat gaagcaaaca
    20701 caaaagacgc tacagctcta tctgaagcaa tggaagctct ctcggctctt ggttacagtg
    20761 atcgagaaat caaacgtgta gaaaaacaat taaaagaagt agataatcag acaacggatg
    20821 agtatctacg ccaagcactc aagctaatga tgaaaaaata aggaggagac caaatgacag
    20881 atgaatactt gttatcacca gaatctggcg aaaatgaact gagtttggaa aaatctcttc
    20941 gaccacagct gctttctcaa tatatcggac agagaaaagt caaacaagaa cttcagattt
    21001 acatcgaagc tgccagaaat cgggaagaac ctttagatca tgttctgttg tacgggcctc
    21061 ctggattagg aaaaacgacg atggccatgg tcattgctaa tgagatgacc gtaaatattc
    21121 gtacgacgag tggccctgct atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac
    21181 tggaagcagg ggatgtcctg tttatcgatg agatccatcg attacctaga gtggtagaag
    21241 agatgcttta ttcagctatg gaagatttct atgtagatat catggtagga caagggccaa
    21301 ccgcacatcc ggttcatttc cctcttcctc cctttacttt gattggcgcg acgacacggg
    21361 caggtatgct ttctgcacct ttacgagatc gatttggcat catttctcat atggaatatt
    21421 atgacgaaca ggatctgcag gagatcgttt tacgttcttc ggatattttt caaacagaga
    21481 tcattgaaga aggggcattg gaaattgctc gccgatcacg aggaacgcct agaatcgcta
    21541 atcgcttgct caaacgggtt cgtgattttg cgcaagtcca aggagacggg aaaatcgatc
    21601 gtcctatagc ggatcaggca cttactttgc tacaggtcga tcaggctgga ttggattatg
    21661 ttgaccagaa attgttgcga acaatgatcg agttatatgg cggcggacca gtagggttaa
    21721 gtacgctttc agtaaacata ggagaagagc gtgagacggt agaagatatg tatgaaccgt
    21781 atctgatcca aaaaggattt ttaaagcgta cacctagagg gcgaattgct actgcttatg
    21841 cctatgaaca ttttggttac gactatcata tatgattttt gcttttagat tggaggaaaa
    21901 tgaatgagtg atacaacacg tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc
    21961 caaacgcaat cttttcgaaa aatcagcgtc caaaaaatcg caaatgaagc aaaaatcaat
    22021 cgacaaacat tttattatca ttttacagat aaatacgatt tgcttcgttg ggtctattgt
    22081 gaggatgcat tgcattattt agatccacaa tgtctttcac ttgataattg ggaagagcaa
    22141 gcattgaaaa tgctgaaagc aattgctgag aagaaagaat tttatgcaaa tactgtgctt
    22201 tctgacccag aaattttaca aaaagagttt tctcaactga tccaaaaatc atttatcaag
    22261 atttttgaac aaatggatga agaaagacaa ctttctgcaa gagacaaact attttatgct
    22321 cgtttttttt cttacggttg cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa
    22381 gaaacaccga tggaaatcgc tatgcagctt ttccgtttag ccaaggatac ggaattatat
    22441 tcttatcgtc tttatgcaaa agaagagttg gaagaacagt aatagaaaaa ttttttgttg
    22501 gaaaagcaat aatcgctaaa aggagatggg gaaatgacaa aaattttatt tgtttgtttg
    22561 ggaaatattt gccgttcacc gatggcagaa ggcttgctga gaaaacgaat agcaatagaa
    22621 ggaagggaag cagaattttt tgtggattct gctgctacaa gcacgtatga agttggaaag
    22681 acaccgcacc ctggaacgaa aaaaatatta aaccaagaaa aagtagatat gacgaatatg
    22741 gttgctcggc aaatcacacc ccacgatttt gaaacttttg attggatcat tggcatggat
    22801 caagaaaatg tagaagaact gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat
    22861 ttgtttttaa gtagcattcc tggaaaagaa aaagaaaatg ttcccgaccc atactatacc
    22921 aataactttg atgagacata tcacttgatc acagaaggcc tgaatcattg gtggaatatt
    22981 tggattgacg aaaataaaat aacaaacaat ttgtaaagtt gacgctttct ttttaaggcg
    23041 atgttagagc atttccttgt ttttttgtaa aatgttaggt aaaataaaat taattaagta
    23101 acactttctt agctaaagga ggagtgcatt cgatgaacga agagacagta tcgccaatga
    23161 agaagcttac gaagttagtt actcatactt cgtatcaact ttttgattct tttgtaaatg
    23221 acactgaaga acatgctaag aatgtacgat tgcaaggaaa atttattctt gagctgcaaa
    23281 aagctgctac tgaacatagt ctagttatct tacaagtaag agcgaatcgt ttgagtagca
    23341 agtatgaaac gatctcaggc tgggtggcga caaaaacggt cgaaaaagac cgagtgatga
    23401 tccgcctaca aaacgaagag cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa
    23461 taacaatcct ttcaccagca ggggaacagg aacgattatc aaaataattt ataccagata
    23521 gaaaagcacg tatgagcttg taccatcaat cattactaat caaaaataac cgaatgcttt
    23581 aagctgatat cagtaaaacg gaacgtttta ggatgagtat cacagcatga cagcattggg
    23641 ttgattagtt gaatcgattg gatggtacaa gtttttttga taaaaaaagg tcctgctagc
    23701 cggggagcta gcaggataag gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg
    23761 tatgcttata taatagtcac tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt
    23821 taagtttttt tgcgaaaact atttttatat catatatatg tctaaaatct atattataaa
    23881 agaaatgttg tttgatcagc ttctcttcgg tattagttca aaacaaaacg gcaaagtata
    23941 tggagctact ttgccgtttt gtttctctaa tactcaggtc agtttataga cgctcaataa
    24001 acggtgttca aaagccagtt tctcggtatt aagtcaaatt tatgcaaaaa tgtgagaagc
    24061 cattttcaaa aaatatttct taatactcga aactgagcgg ctttttcacg acctctcaat
    24121 aaacagtgtt caagagacag cctatcggca ataaggctga acgtctcttt cacaacctct
    24181 tttatcaatc tgctacttct tctttcttag cgaaactcca aatcagtccg ataacaaaac
    24241 cgataactgc tggtacgatc caccccatcc ccaatttgaa aaacggcaag tagctttccg
    24301 caacatataa aagattttga acaataggtg tttgtttgat agtttctggg caagcgttca
    24361 acccatcaaa aatcgaagca atcaatgtaa aataagtagt cattcgataa acagccgtac
    24421 gctgcttaaa caaaggacca actaacacta gtaaaatcag tgtcattgct aatggataga
    24481 tgaacatcaa aacaggcaac gaaaattgaa tgatattcgt caagcctacg tttgccgcta
    24541 aacaaggaag gatactagca agtgcaacaa agaatgcgta atttctttgc ggaaacaaat
    24601 ctgtgaatgt ttctgcaaaa gctgtaatca aaccaatacc ggtcttcaag caagctaata
    24661 taacgataaa tgctaataag atactgccat aagtacctaa ataatgatca gcaatctgag
    24721 ccagcgtgat cccgccattt tcacttagag caaagctacc taagctcata gcacctaaat
    24781 aagcgagcaa agtataaatg attcccatca atataatgct gatcgcacca gatttgatcg
    24841 tgtctttagc gatttcagca ggttttgtta cacccatgtt ttggattgtt gtgacaatga
    24901 tgatcccaaa tgctaaagca gcaagcgcat ctaatgtgtt atatccttga gtaaaccctg
    24961 tgaaaaaagc atgctcttga taatttggct gaataggcgc ttgatttaca gaacctagtg
    25021 gattcaaaaa agcaaggaga agcaaagcac ctaataaaac caaaaatgca ggattcagga
    25081 actttccaac ataatctaat atctttgtcg gttttcttga aaaccaccaa gccagcacaa
    25141 aaaatacaat actgaaaatt gctagatata gcgtctgtct gctaggatcg ataaatggtg
    25201 ctaaaccaat ctcgaaagat gtggttgcaa ggcgaggtaa agcaaaaaac ggtccgataa
    25261 ctaagtaaag caagatggta aaaaccatcg catatgtttg atttatccgt tttgctaatt
    25321 catatacacc tgagcttttc gatacaccaa tggcaatcac ccctaagaag ggaagaccga
    25381 taccggtaac caagaaacct agattcgccc aaaaggtagc agcgcctgct tcttgaccca
    25441 aatgaacggg aaagataaga tttccagcac caaaaaatag accgaaaagc atggagccta
    25501 taaaaagata gtctttaaat gataattttt ttgtcataca taactccttt acggtttttt
    25561 taaaagtaac aaaatttcga aaaatatgca acaattttca gaaaatttcc tatataaaac
    25621 gacggttatt taataacgta agtgataaaa taaaagcgct gaatcatctg aggaggaatc
    25681 atcgtgacaa agtcgagatt agaagcattt actgatggag tggtagccat tgttttgaga
    25741 gttcttgtat tagagatcaa gatccctaat acatctagct ttcattcttt atgggtcatc
    25801 cgaaatacat tattagctta taccatcagc tttatatttg ttgcggtgat ctgggtgaac
    25861 catcatcgta tatttcagat ggcagaacga attaattacc gagtggtctg gtcgaatatc
    25921 tttttgctct tttggctgac tttatgtcct tctgttacta gttgggtcgg gcgtaaccct
    25981 gaagcttttt ggcctgagat ggcttatgtc ttagtataca cgatgtggag tttttctttt
    26041 ggtatcttga tgaggcaaat catgaaagca aataaaccag attcccatgt cgtgagagtt
    26101 ttgtcaaagg accgtcgaag tcttatctcc atgatgatta acttatgctt gattgccggt
    26161 gtcttttttg taccgttgat cgggttattc ggacgttttt ttgtatctgg tatttggatt
    26221 ttttcctata aaaaagcaga tgcctattat cagcggttgt ttccggggaa atagaaaaga
    26281 ggctgggaca gaagctttta actccaagaa ataagccgaa atttcacaaa aatttaagaa
    26341 gcaattttcg taaattcctt cttatttctc gaagctgacc acttctgtca caactacttt
    26401 cttacttctt acgttcatat ttcatatgat agattagtaa tagaagcaag aaccacacag
    26461 gtgttagtaa taaagcgatt ctcgtatctt ttcctaggaa caagacgaca cacacaaagg
    26521 ccatgtaaag aaataccaga taattactaa atgggtacaa cggcatttta aatgaatgag
    26581 cttttttgcc gaggatactt ttacgatatt tcatgtgcgt gtagacaagc atactccaga
    26641 taaacaagaa acaagtagtg gcaatactgc ttactagtgt gaatacttca ctaggcatca
    26701 cgtaattcaa gataacagaa atgaaaatga cgatagtaga aaatgtcaaa gcattccctg
    26761 gcacatgatg tgttgttaac tttttgaatt ttttaggggc actgccttct tgcgctaaag
    26821 aacgaagcat acgtcctgta ctatatattg cactgttgca ggcagaagct gctgcactta
    26881 atacgactaa attgatcaag gaagcagcaa tcgtgatccc aatttccgaa aatacttcta
    26941 caaatggact gttatctgga ttcaagctgt tccatggata gattgccata atgacaaaaa
    27001 gagatcctaa gtaaaataag atgatcctga taggaatatt attgatagct tccggcaaca
    27061 ctttttcagg attttctgtt tctccagcga ctaatccaac taattctatc ccagcaaaag
    27121 caaaggttac catttggaaa gctaagataa atccttttgc accagttgga aagaatccgc
    27181 catgagacca tagattcgtg atactggctg tgccaatatc tgtcgtatag tgggtgagga
    27241 tcatataagc tcctacgagg ataaaggcaa tgatcgctac aactttgatc aatgcaaacc
    27301 aaaactctaa ctcaccaaac aatgagacag ccaccatatt caagcaaaga agaagaccta
    27361 atgcaatcag ttcaggaagc cactgaggaa caccaggcgc ccagtagcgt acatacattc
    27421 cgatggctgt taaatcggcc attgcaatcg atacccagca gaaccaatac gtccagccag
    27481 tgatgaaagc cgcttttttg ccaagatatt ctgcaacaaa atccagaaaa gaatggttat
    27541 tggtgtttga taacagaagt tcgcctaacg cacgcataat gaaaaaacag atgcatccag
    27601 taataagata ggcgagcaat atcgaagggc cagctagctg gatagacttt ccggaaccta
    27661 aaaacaaccc cgttccaatc gctccgccaa tagaaatcag ttggacatgt ctatttttca
    27721 gtccacgctg cagttcttgc tcttcattca tttttaaatc atcctaacta aaaaagttta
    27781 attacttcca tattaaaaga gttttctgaa aaacacaact aagattccaa aattttcaga
    27841 aatttacctt ttatgagtaa ttcataaaaa agaactgcgg taaacaccgc agctctttgt
    27901 cccaatcagc tattgatcct aagcatttaa aatacgtgaa aggaattctt ttgtacgttc
    27961 ttctttcgga tggacaaaga tatcttctgg tgtaccttct tctgcaatca cacctttatc
    28021 cataaaaatg acacggtcag atacatcttt tgcaaattcc atctcatgcg tcacgataag
    28081 cattgtcaag ccagtatgtg ctaagtcttt gatcgtatta agcacttcgc caaccatttc
    28141 agggtctaac gccgaagtag gttcgtcgaa tagcaataca tctggattca ttgataatgc
    28201 ccgagcaatt gcgacacgct gtttctgtcc accagataat tgtgctggtt tggcttcgat
    28261 ataacgatcc ataccaactt tttccagatt ttcgattgct acttttttgg cagtttcttt
    28321 atctcgtttc aggacagttg tttgaccaga catacagttt tccaagacat tcatattgtt
    28381 gaacaggttg aaggattgga aaaccatacc tagattcgtt cgatatttag gtaaattata
    28441 tttaggagct aatacatttt ctccattata aatgatctcg ccgcctgttg gtttttccag
    28501 taaattgata cagcgcaaga aagtagattt accagaaccg gaagaaccga tgatcgtcac
    28561 aacttcacct ttattgactg ttacgctgat atctttcaat acttcatttt caccgaagct
    28621 ttttcttaga tgtttgattt caataatttt ttcattcatg attcttcctc cttattcctg
    28681 caaatttgaa ttgtcgattt cttcaagctt ggtataagct gaaggaccat cgattttctt
    28741 ttctaccgcc cgcaggattc tggtgatcgt aaaggtcatg actaagtaga tgatcccgac
    28801 gatcgtaaat gtttggaaga attggaaatt cgcaccggaa gcagagtttc cttggaagaa
    28861 caaatcagct acgccgatta cacttaaaac tgctgtatct ttgatattga taacaaactc
    28921 atttcctgtt gctggcaaga tattccgtaa aacttgtgga ataacaactt ttctcatcgt
    28981 ttggccatgg gtcataccaa tggcttgcgc agcttcgaac tgcccttcat ctactgcaaa
    29041 aatcccccca cgaacgatct ctgtcatgta agctccagta ttgagagaga caataaacaa
    29101 agcggcaatc gttcggttca atgaaatgcc aaaagcgagt gccagaccgt agaagatgac
    29161 cattgcttgt accatcatcg gtgttccgcg gaatacttcg acatagatag aaagaagtgc
    29221 atttccgatt ttttggaaga agcgagtcag tgcatttcct gattcaggta ccgaacggaa
    29281 gacaccaatc agtaatccaa gtgtagtacc gacaactgtc ccgatcaatg cgatgaacaa
    29341 ggtaagtcca gcacctcgta agaacatatt gccgtattgt ttccaaatcg tagtgaaatc
    29401 agaaatgatg ccaggttttt cttcactatc agttgcagca ggctgatctt tgactgcttg
    29461 gtccataatt ttggttcgtt catcgttaga gatgccagaa agaatctggt ttactttttg
    29521 gatatccggg tcgcctttac gcataccaac agaaatctga acatcttccg ggtttgtctg
    29581 gaagccatct gcaccgctga attcaagcat ttccaagtct ttgttcacac tagttgccgt
    29641 tacaccttcc ggacgttcgc tgacataacc atcgatcatt ccagaagcta aagctgttct
    29701 cattgctgag aaattatcca ttgcttgttg tttctgcaca tctggtatct gatcgatcac
    29761 agtatagtgg aatgtattta actgagcagt aatctttgct ccagaaagat ctgctaaact
    29821 ttttgctttt gcatatttgc tatctttttg aactactact actaattgtg attcgtaata
    29881 aggatcggta aaatcaattt cttttcgtcg ttcagcagta gggctcattc cggcgatgat
    29941 cgcgtcgatc ttgcctgatt gaagggcagg agccagacca tcccatttcg tttggacgat
    30001 gactaatttt ttccctaaac catctgctac ttttttagcg atttgaacat cataaccgcc
    30061 ggcgtaacta ttttggcctt gaattggcaa ggcattattc tcatctgttt gttgagacca
    30121 gttgaagggt gcatacccag cctccatacc tacgcgaaac tcatcgctcg gtgttttttc
    30181 atctgcattt gccagagatc ccacactgaa tagtgagaag acaaaagcaa atagtacggc
    30241 aactgaaacc atattttttt tcattttttt ctccctttct gattatatga tacacgagcg
    30301 gtctagatga aatgcggtaa agctttatct gaaaaaataa aaagcgaccg atttatagat
    30361 ataaaaaggc cgctaaaatt ccataaggtg ataaacctct cacaaaacat agcgcaactt
    30421 agcatcactg ctaagacagt ccgcaagctc ttaacttgcg tcccaacata cactccaagc
    30481 aaaaaagtgc acatttcggc gatcctccct attcctgctt ctccatgttt gcttcactcc
    30541 acagggttaa tgattctcgc gacctctacc tcattgattg aggcatctcg tattcagtta
    30601 tttatacaaa aaaagtttac gatactcgag aggctttgtc aatctataac tgctgttttt
    30661 tgaaaaaata ctgataaata tagaaatctt tacttttacc gaaaaaacaa tcaaaatgtg
    30721 aatcatctat ccattgcaaa cgatagtata attatacaca aaaatgtatg taatacaata
    30781 aaaaaagaac tagaacagaa aatttcctgt gtccagttcc atctcgatca tttaagctgc
    30841 cgctttcttt ggctcacgtc ctaggatcgc ttgcaaaaga acaacgacgc cataaatggc
    30901 taatattaga taaatcaaac ttgagtattg ggtgattccc cagccccatg ttcgatatac
    30961 ggctaatgta atggctaaaa caattgcggc aatcacgttg aataaagcaa aagcccaaag
    31021 atttccattt tctttgcgta caagataaaa aacagagaaa taaacgctta acgctaacca
    31081 ggcgatacaa gccagaataa cgccccaata aataaagaat ggtaacatat cgattcacct
    31141 cacttttata cagcagtatt gtaacacgtt ttcattcttt tgtcagtgaa cattgtgata
    31201 aattgttcaa atgcccataa cactttttta ttcccatttt tctgactttt caacaataaa
    31261 tttgttcgag tatgggctat aatggaaagt gaggtgacaa aacatgaaac aattaacagt
    31321 agacgaagca gcaaaagaga tattagctta tcagcaaatt acatttttga ccggtgcagg
    31381 ggtatccaca ccttctggag tgccagacta tcgatctttg tcaggaatct accacgggat
    31441 ggagcagccg gaatacttgc tgagtcatca agcaatggtg aatgaaccgg ataaattcta
    31501 tcaatttgtc aaaaagattt atcatcctga agcaaaaccc aatatcattc atttggaaat
    31561 ggcacatcta gcgaaagaaa aaaatgtttg ggttgtttcc caaaacatcg acggtttgca
    31621 cgcaaaggct aaaagcccgc aactcgtcaa ttttcatgga agtttgtatc aatgtcattg
    31681 tcgaaaatgt caacaaacgg tagactggaa agaatatctt cacagcgaca aacatcaagc
    31741 atgcggagga caaatacgcc cagacatcgt gttatatggt gaagggtttc aagatggagt
    31801 catggagcag gcggcttatg ccgtcagcca agcagagctg atcgtcattg ttggaacgag
    31861 ttttcaagtt catccgtttt gtgatctggt acagtttcgc cagccgtcag ccaaaatctt
    31921 agtaatcaat caaacgcctg tttatttagc ggagccttat acgtttattc aagaagacgg
    31981 agcggttgtt ttcaaaaaaa tacaagaatt aggagcagat tatgactgaa ttcaaaaccg
    32041 aaaaagaaaa aatgattgca ggcgagcttt attttgccaa tgatcctgag ctggttgccg
    32101 accgtatgtt tgcaagaagt caaagccaaa tcatcaacca agcagaatca gcggaactgc
    32161 gatcccaatt gttgaaagag acttttggcc gtacagggaa aaaaatctat atggagccag
    32221 ttatcaattt tgattacgga tataatatat ttgttgggga gaatttttat gcaaatttca
    32281 attgtacatt tttagatgtc agcacgattg aaatcggtga taactgcatg ttcgctccga
    32341 atgtccagct ttacacggcg acgcatccac tacatccagt gaaacgtaac agcggtctgg
    32401 agtacgcaaa acccattaaa atcggtaata atgtttggtt aggcggagga gttatcgtca
    32461 cgccgggtgt cactctaggg gacaacgtag tagtcggcgc aggttcggtt gtgaccaaat
    32521 cttttcccga caatgtagtg attgcaggaa atcctgctcg tatcatcaag acagtagagg
    32581 aagaactaac agaagagtct cttgaaacat tacgtcataa gatcgatatg atcgatcgag
    32641 agttgattgc tttattagaa aaaagaatgg atacggtaac taaaatagga caagtgaaaa
    32701 aagaagaaaa ccaagcagtt tatgatgcaa aaagagagca gcaagtattg gataaagttg
    32761 ttagtctgct gaaaaataag ggatataagg aaacaataac agatacgtat gttgatttga
    32821 tgaaacactc gcgcgaatac caaaacaaaa tgaaggagga ataagtgtga tcgaaggaga
    32881 gacacggcga aaacaaatca tgcaaacctt ggcaaataca gaaaaaccag tgagtgctag
    32941 caagtttgcg aaaacttttg gagtcagccg tcagatcatt gtaggcgata ttgctttact
    33001 acgagctgca ggtaaaagta tcgtggcaac agcaagaggt tatatcctcg aaacagaaga
    33061 aaccaatact gggcacatca gtaaaatcgc tgtccagcat ggaaaagatc aaacagaaga
    33121 agaactgaga ctaatcgttg aaaatggtgg tgaaatcatt gatgtaattg tggaacatcc
    33181 cctttacggt gagttagcag gaactttgca catcaaaacg ccacaagata tcgattcctt
    33241 tatgagacgt tatcaaaaaa gtaatgctac tttgttatcc gaattaacag gcggcatcca
    33301 tctgcatacg atccgctacc cagaaaagca tatgctgaaa aacatcaaac aaaaactagc
    33361 tgaagcagga atcctctacg aaggataaca tcgtagctgc tactttttat cctagaaact
    33421 attctatcat ctctgagata aacacccata tggaagaaat aagaaaaaat aagaaaagat
    33481 gcgtatccaa tacacagaat tttttggaat tgcgatttct agatgtctat ctaaatttcg
    33541 attagtggag aaacataaaa aagggaagtg cagcttttga gcactcccct tttttataaa
    33601 gctttcttgt agcatagtat cgaaggaacc tgccaattct ctactcgatc accaatacgc
    33661 cttcttttag tgcaaatgga ttttgatcgt tgatgtggtc atagaacatc acaccgttga
    33721 tatggtcgat ttcatgttga acgacgatcg attcataatt cttcaaacga atcttgtgtt
    33781 tttcaccatt catatcataa taagacacag tgattttggc atggcgaaca acatatcctg
    33841 gtacttctcg atcaacagat aaacagcctt caccttcccc taaacaagca tcttgaacag
    33901 aatggctcaa aattttagga ttatacataa cagtacttag agaaggtcca tctgcctctg
    33961 ggtcaggact tggtacgtga acagcaatga ttcttttaga aatatctaat tgaggagcag
    34021 ctaagccaac accgcctcgt aagtgtagtt cttcagcttt gattggatct tggctgtttt
    34081 ttaagaactc aagcatctct tttcctaaag agatatcttc ttcgcttaat ggaagagaaa
    34141 cttcttttgc aacttcgcgt aaggtcggat ttccttcacg aatgatatca tccattgtaa
    34201 tcatggaata tgtacccctt tcttaaaaga ctgtgtttca agtctatttt cgcattatag
    34261 tttaccacaa aaatggaaaa gtagtagtaa gattgtttga aaactacctt ttcttaatga
    34321 ttaactactt gattttcaac ccggtttcag gtaaagtata gctgtgtgct ctaggcacat
    34381 gacctgccac ttggttaagc gaatcaccaa cgttttactc agtcgcaggg gaataatcga
    34441 aagaggtgaa agaaatggca atttcaaaag aacgcaagaa tgaaatcatc aacgaatacg
    34501 ctcgccatga aggggatact ggttctccag aagtacaaat cgctgtatta actgaagaaa
    34561 tcaaccactt gaatgaacat gcacgcgtgc acaaaaaaga ccatcattca taccgcggcc
    34621 taatgaaaaa agttggtcac cgccgtaact tgttagctta cttacgtaaa actgacgttc
    34681 aacgttaccg cgaattgatc aaacgtttag gcttgcgtcg ttaattttag tcaaaaagag
    34741 tgggattcac atgaatctca ctttttttac tcgtatatca attaagtaaa attccgtgat
    34801 caagctagat ggactcctac taaccaaatg aaagctgctc aaaagacggc tttcggcagt
    34861 tttcatttgt ttagtagcgt cttgctttca cgagaaggaa aaggagaagt tatgtcagaa
    34921 aaacaagtat tcaaaacgac atggggcggc cgtcccttac aaatcgaagt cggtcagcta
    34981 gcaaaacaag caaacggagc cgttttagtc cgttatggag atacagtagt cttaagtgca
    35041 gctgtggcct ctaaagaagc aaaagataca gactttttcc cattaacgat caattatgaa
    35101 gaaaaaatgt atgcagcagg gaaaatccct ggaggtttca tcaaacgaga aggccgtcca
    35161 agtacggaag caacattgac tgcccgtttg atcgaccgtc caattcgccc gatgtttgca
    35221 gaaggcttcc gtaatgaagt ccaagtaacg aacatcgtga tgagtgtaga aacagactgc
    35281 tcgccagcta tggcagcaat gctaggttct tctttagcac tatcaatctc agatatccca
    35341 tttgacggcc caatcgcagg cgtagaagtt ggtcgtgtaa atggcgagta cgtcttgaat
    35401 ccaacagtag aacaagcaga acaaacagat atcgaattga ctgttgccgg aacgaaacaa
    35461 gccatcaata tggttgaaag cggcgcgaaa gaagtttcag aagaagacat gcttggtgca
    35521 ttgctgtttg gttttgacgc tatcaaagag ttagttgctt tccaagaaga aatcgttcaa
    35581 gcagtaggga aagaaaaaat ggaagtgact ttactgcaag ttgatgaagt attgaaaaaa
    35641 gaaatctttg atgcttctta cgctacaatg aaagcagcag taatgactga agaaaaactt
    35701 gctcgcgaag ataatatcga gcaggtgaag atcgatatcc gcgaagctta tgctgagaaa
    35761 tttgcaggtc atgaagatga agatcacttg ttgaaagaag ttaaacaaat cacagaagat
    35821 cttgaaaaag atgtcgtacg tgaattgatc accatcgata agatccgtcc tgacgggcgt
    35881 aaattggatg aaatccgtcc attgtcatca gaggtcagct tattgccacg tgtacatgga
    35941 tcaggtttgt tcacacgagg acaaacacaa gctttatcag cttgtacact cgcacctcta
    36001 ggagagcacc aaatcattga tggtttaggg gtggaagtca gcaaacgatt catccaccac
    36061 tacaattttc ctcaattttc tgttggttct acaggacgtg caggctctcc agggcgtcgg
    36121 gaaatcggac atggcgcatt aggtgaacgt gcgttagcac aagtaatccc ttcagaagaa
    36181 gaatttcctt atacgatccg tttagtagca gaagtattgg aatcaaatgg ttcttcttcc
    36241 caagcaagta tctgtgctgg cacattagca ttgatggatg caggtgtacc aatcaaagcg
    36301 ccagtcgccg gtattgcgat gggacttgtt tctgatggtg aaaactatac gatcctaact
    36361 gatatccaag ggctggaaga tcatcttggc gatatggact ttaaagttgc cggtacaaaa
    36421 gatggtatca ctgctttaca gatggatatc aagattcaag gaatcacaga acaaatccta
    36481 actgaagcat taactcaagc aaaacaagca cgtatggaaa tcttagaaga attgacttca
    36541 acgattgctg ctccacgtga agaattaagc caatatgcac caaaaatcga aatgatccag
    36601 atcgaacctg ctaaaatcaa agatgttatc ggtaaaggcg gcgatacgat caacggaatc
    36661 atcgatgaaa ctggtgtgaa aatcgatatc gatcaagatg gaaaagtaag tattgcttca
    36721 gctgatgcag aaatgatcaa aaaagctatc aagatcatcg aagatttgac caaagaagtc
    36781 aaagttggtg aagtttatct agggaaagtt gtccgtatcg aaaaattcgg tgcattcgtc
    36841 aacttgatca aaggaaaaga tggacttgtc catatttccc aattagcaaa cgatcgtgtc
    36901 aataaagtcg aagatgttgt taaactaggt gatgagatcc ttgtgaaagt aactgaaatc
    36961 gataaacaag gacgcgtaaa tctgtcaaga aaagcaatgt tgaacgaaga tggttcaacg
    37021 aaaaatgaag aaaaataatt taattcatag gaataatgta cttgtgatcg tgaaggtgac
    37081 agggggacaa taattattcg aaaaaagggg actgtgacaa aagccgtgtc acagtccttt
    37141 tttttcgaat aaacggtgtt caaaagcttg catctgcggt aataagtcca gccaagttaa
    37201 aaacattagg agctgttttt atttggctgt tcttattact tgatgcagaa cagctttttt
    37261 acaacctctc caataaacga tgttcaatca gctgtccctc gatattagct caaaaaacgg
    37321 caaaatatga ggaactattt ttccgtttgt ttctctaata ctcaggtcaa accgctgatt
    37381 tcacaacctc tgattcacgg tgttccagaa gcagcttcta cggaaataag ctgaacactt
    37441 ttgttacaac ttcttttttt ctggagtttc ttggcgcata tttttggagt ccttctgaat
    37501 aaagcgtaaa ataaaagtga gaaatatgga taaaggaggg aaaggagatg ctgaattttt
    37561 tatggtcgtt atttatcggc ggtatattag gtttgatagc tggcgcaatt ttaggaaagg
    37621 aagtcccggg aggcgtatct ggaaatatca ttatcggttt tttaggtagt tggctcggcg
    37681 aatttttact aggaccactg ggaccggaac tcggcggttt ttatttgata ccagcattat
    37741 tgggtgcaat tttttgcctt gctctttatt catttgtggc aagacgtatc agagaacgtc
    37801 caaagaaaga ataattttat tcaaataagt tgacacttca gtgtgttact tggataatta
    37861 gaagcaattt aggaaagcgg gtgtcatgga taagaatatc atctatcatt caagcttacc
    37921 taaaggatat ccatataaca agaaatgagg gaaaaacgtg gcaacaacgc acaataccaa
    37981 aaagatatca atggctggcc tgcttgtagc gatgggagtc gtctacggag acatcgggac
    38041 aagtccgctt tacgttatga aatcgattgt tgaagataat ggcggattgt caggaatcaa
    38101 tcctgatttt gttatcggat cagtctcatt gattttttgg acattgactc tactgacaac
    38161 aatcaaatat gtcgtgattg cattgaatgc agataaccat ggtgaaggtg gaattttctc
    38221 actctacaca cttgtccgaa aaggtgggaa atatttgatc attcctgcaa tgatcggagg
    38281 agctgcctta ctagcagatg gggtattgac ccctgctgtg acggtaacga ctgcgattga
    38341 aggcttgcga ggaattccgc agttttttga ccggttcggt aataaccaga acgtgatcgt
    38401 cattatcact ttagttatta tttttgtttt attcatggtc caacgctttg gtacagatat
    38461 tgtcggaaaa gcattcggac caatcatgtt cgcttggttt acttttttag gcgtaatggg
    38521 attaatgaac ttcagtcaag actggacagt tatccgtgca ctgaatcctt attatgctat
    38581 ccagctgctt tttagtgcag ataacaaatt aggcttgttc attcttggaa atattttcct
    38641 ggctacgaca ggtgcagagg cgctttattc cgatcttgga catgtcggca aacgaaatat
    38701 tcgtctgagc tggccttacg tcaaaatttg tttggttatc aattattttg ggcaggctgc
    38761 gtggttgttg aatgtttatc aaaaccctga agcacagcaa atcaagaacc tgaatccatt
    38821 cttccaaatg atgcctcaaa gttggatcgt gatcggcgtt gcttttgcaa cagtcgctgc
    38881 agtcattgct tcgcaagcat tgattacagg ttcgtttacg ctagtttcag aagcaatcaa
    38941 gttgaaactg ctgcctcggt taaagatcat gtatcctggg aacagtatcg ggcaaatgta
    39001 tatcccagca gtcaatctga ttttatggat cgcctgttct ctagtggttg tcacattccg
    39061 aacgtctcat catatggaag ctgcttacgg tctatcgatc actgtgacga tgttgatgac
    39121 aacagcactg ctttactttt atcttatcca aaatgggcat tctcgttggc ttgcatattt
    39181 agttaccttt ttcttcggtg cgatagaagt cgttttcttt atctcaagtg ttgtcaaatt
    39241 cttccgaggc ggttttgtag ccgttctgat tggtttgatc atcttagtcg tgatgtttat
    39301 ttgggaacaa gggaacatta ttcgcgaatc gtcaaccgag gatgtgtctt tgagagatta
    39361 tatcccgcag cttagagaat tacgtgatga tacttcttta ccaatctatc agacaaatgt
    39421 ggtcttcttg gttcctgata tggctgaaga taaagttggc cgacagttca tctattcgat
    39481 tttagataaa cggcctaaaa gagcacgagt ctattggttt gttcatgtgg aagtgacgga
    39541 tgagccatat acgaaagaat atcaagtaga tatgatggac actgatttta ttgtccaagt
    39601 gaatctgttc ttaggtttca gggtccaaca ggaaatcaac gtatatgttc gccaagtcat
    39661 ccatgatttg atgaaacaag gccgtttgcc aaaacagcca caaagatact ctctgacacc
    39721 agggcgagaa gttggcgatt tccaatttgt cctgattgat gaagtagttt ccaatgtgac
    39781 aactctagga aaatggcaac gtcaaatcat gcaggcaaaa ctggcaatca aaaaaatcgc
    39841 tacctcgcca gaaacatggt ttgggctaga gtacagcgaa gtaaaacatg aaactgttcc
    39901 gcttatcatt ggaaatactc ggaaaacatg gttgaaagag cggttgtgaa agtagcgctt
    39961 tgacctgaac taatgtcgaa tgtcagctac ttgaacaccg tttatcagag gtcatgaaaa
    40021 agctgtactg caacaagcag taagaacgag cagataaaaa cagcttctca tgtttttcat
    40081 ctgtttgggc ttaatgccgt agatgcaagc ttttgaatac cgtttttatc agaggtcatg
    40141 aaaaagctgt ttagcaacaa gtaacgcaat gattttgaag tgataaggag atgtggctga
    40201 aaaatgccgc atctcctttt ttattcctaa atatgaatac gcattatttt caatcttttt
    40261 gtagaaaaag ggtacaatag aaaaagagaa aggcgggaga atatggttat caatttcatt
    40321 attttatttt tgatgattgc aattacttca ttttttgtag caagtgaatt tgctttagtg
    40381 aaaatcagac gatcaagatt ggaacaacta gaaaaggaaa atgtaaagaa cgccaagcta
    40441 gctcttcatg ttacacacca tttagataac tatttatcag ctagtcaatt aggaattacg
    40501 ctaaccggtt tgatcatcgg gtgggtcggt gaaggatctg tcgcggcttt actagaacca
    40561 gtgatcggta aactaccttt tagttcggct attagcagta caatctctgt ggcgctggga
    40621 tttattttag ttacgtatat cgatgtggtt attggcgaac tgcttcctaa gagctatagt
    40681 atcgtcaata cggagaaggt cgtcttgttc gtagttaaac cacttcatta tttttataaa
    40741 gtaatgtttc cttttatttg ggtattgaat cattcagctg cagggctagg gaagctatta
    40801 ggtgtgcgtc tcgtatctga aggagaagag acattgtctc aagaagagct gacattagtc
    40861 gcattgaatt cttatgaaaa aggagagttg accaaagaag aatattatta tttggaaaac
    40921 gtgtttgaat ttgatgatac cttggcaaaa gatatacaag tagaccgtac gtccatgcaa
    40981 gtattcgaag cttcagaaac cgtcaaagaa gcgataaaag cttcgattga acaaggacat
    41041 actcgctatc cggttatctt agaatcaaaa gacaatgttt taggttatgt caccttgcca
    41101 gatttgatca agcaatctta taaagatgac cagctgacag tagaacagtt gattgaagag
    41161 ccgatcgtaa caactgaaac gatccctata aaaaaattat tgacgatcat gcgaaaaaag
    41221 ggtaagcata ttgctatctt aaaagatgaa tacggaggga caagcggctt agtgacgatt
    41281 gaagatattt tagaagaaat cgtcggggaa atccgagatg agacagattt ggacgaagca
    41341 ttgatagcgg aacagtcaga tggctcttat atcatctccg gcaaactaac attagatgat
    41401 ttccagcgtt attttcatgt agagattcca gagtttgaag aaacgaattt tactacactt
    41461 gcgggctttg cttctagccg atataaagaa ataaaagcag gaacaatcat tgagattgca
    41521 tcattccgtt tcacggtatt agaataccag catgcacata tcgattattt caaagtagag
    41581 tccacggaaa gaaaaacaga ataaaagaaa aaaggctgtg acattttttg tcacagcctt
    41641 tttgttatct aaaaaacctg ttttcggtaa acgaagaaga tattttcaat aaaagttttc
    41701 atttttagcc gcaacgattt cttttaatca aggctatctt gcttctgcat gttctaatgt
    41761 gaaatgttcc tcttcaccca tgtgtttcaa ttgtttcatc gaacgcatat aagagtaaag
    41821 caacacggca acagaaccga tccaagctaa aggagaagct gctgctgctc ctgggtatcc
    41881 ccacattcct gtaagaacga tagctgcaaa agaacgcata agcaattcca tcataccggc
    41941 gatcgtaggg atcttgcttt gtccaagccc ttgaagtgtg tagcgtaaaa tgaacaggac
    42001 tgctaagatc cagtaaagag acccattgat attgaagtag gtttgtgcta attcaaatac
    42061 acgtgtttct gatgggctaa caaacaattg aacaaagaaa tgaccgaaga aaatgacgat
    42121 agcacctgct aagagactaa atccgacact catcagtaaa gtttgacgta ccccttgcaa
    42181 gattcggcca tattctttcg caccatagtt ttgtgcagtg aaagttgcca ttgcaatacc
    42241 aaatgacatc atcggctgat ttgcaaattg gtcgatacga ccagcagcgg cttgagcagc
    42301 tacgacatct gtccctagac tatttagtgc tgcttgtaac acgatagcac caacagcgat
    42361 gatcgaagat tgaaacgcca ttggcaaagc agcatttagg tgtacagcaa actcttcttt
    42421 ttcaaatttg aaatcttttt tgcgtaactg taacaaaggc atttttttct tgatatacac
    42481 tagacacagt acgctggaag aaacttgtgc gattactgta gcaatagctg caccttccac
    42541 acccatgcca aaatagataa taaagatcaa atcaagtacg acgttgatga tgaccgcaat
    42601 gatcagaaag aatagtggtg tgcggctgtc accaagtgcg cgaatgacat tagaaagcaa
    42661 attgaaactg actgatgcaa agatccctaa taaaataatt gaaataaatg tttgtgcttg
    42721 atcaagtatt tcaggaggag tttgcatcaa ttgaagcatc ggacgaataa acaggagact
    42781 taggacagtt aaaatcacag tgacgacaag actaatgaca acactagcag caaaactttt
    42841 cttcagaccc cgataatctt ttgcaccgta acgttgtgcg gtaataatgg ctaaaccagc
    42901 tgttaaacct tgggcaaagc caatgatcaa aaatgtcagg cttccagttg caccaaccgc
    42961 tgccagcgca tttttaccta gcgtctgacc cacgatgatc atatcgatca tattgtagaa
    43021 ctgctgaaaa acatttccga tcagtaaagg aacagtgaac atcaaaatca gtttagcggg
    43081 gcttcctttt gttaaatcgc gcatctttta aactccttat atctttaaaa tttagttagt
    43141 agtatatagc gtttatcctg agaataccaa ttagaaatca atgataattt atgaaactcc
    43201 ataaaaagaa gccaagaatt gagtcaaaga atcaacaact tggcctggtc tgtaaaataa
    43261 gggacatcca cgtttatcta tcaccttaat attgtctaat ctcacttggt aaaaggatcg
    43321 tttctctcgt accgtcatca taggcgtata cttgagcagg aaattggttc gaataggcaa
    43381 acggcaaatc aatatccatt tccacattgc ttcctgcttg agaaaaatgc atcgtcactt
    43441 ttccacgatt ttcgatcaaa tcaaaggtta atatattttc taatggaatg actcccttaa
    43501 gatctaggtc aataataaac catatactgt caatcaattc atctggcaaa cttgatacga
    43561 caccaactga agcgtaacgg ttgcggctgc tatcaaaact ttcaaacatc tgtttaacct
    43621 ccttcgaatc aacgaatgtt ttatttttat aattaatcct aggataaact agaaatactt
    43681 acttcaacga gggttcattc taataaagaa tcaggaaaaa tgcaacaata attgaatact
    43741 ttttttcaaa gtataaattt ttataaaggt acgataaaag tatgcgagga aaagcttggt
    43801 aaattaggat ttttcgtttg taaagccatt tacaaaagtg gaagccccat atgaaaataa
    43861 acgattagaa ataaaaaagg cgtgttaaaa gctgctgacc cgccgcttct aacaccacct
    43921 gattgtttca gtattattct tctgttgaac cgatcacttc tggttcgcca ccttcacctg
    43981 tgctttcttc ttcttcactt tgagcgacta cagcagcgat ttgctcttca ctgtcggtca
    44041 cgaatttgta atcgtcgttt tttggaagat cggctactgt aatagaatca cctaattcca
    44101 agtttgtcac gtcaacttca attcgttcag gcaatttgtc tggtgtagca gatacgacta
    44161 ctgtatacaa gttttgagct aacactccac cagatttagc acccactgat tcgccaacaa
    44221 gaacgacttc tgtttcgact tctgtttctt ccgacatgtt tacagataga aattctgcat
    44281 gttcgaaatg attggtaaaa gtgttcgttt ggatcttgtg aaccaatgta ttgacttttt
    44341 gtccatcgat gtccatcgtg atgactgtgt tcgctccatg ttcacgcaac aatttgctta
    44401 gtgtcaaacc atcgaatgag ataggggtac tttctacttt atacccataa acaacagctg
    44461 gaactttccc ttcgtgacga agtttgtttc ttaatgaacg gggacgtact tctcttttac
    44521 ttacttctaa tgatactgac ataaccaaaa ttcctcctta aaatgtgact gtctgctttg
    44581 gtaacggaca gattttattt aagtgaattc taaaggctaa tctcgtgcac atcatcgaag
    44641 gcagggcgct taaaaaagcg caaaaggaga gatgcgcgca tctcccctta gaaagtcagg
    44701 attcaacttt tctccaccag gtcattacgc tgtgtatgag aaatccaatt cactttacta
    44761 tttaaccgta acgtgaactg ttaattaagt caaggaataa ctatgatgat gtatttgcaa
    44821 gcaagaatat tcaaggtata attaattagt agtaataaga aaggaaacag catgcgttta
    44881 gataaattaa tagaagaaaa attagaaacg acacgcaagg aaatgaagcg gctgtttgct
    44941 ttgaagcaag tgatgataga tggcacagtc gaatttcgac aaaatcgaaa tgtagatagt
    45001 ttactgcatc aaatcgaagt agcaggtgaa cacttagaaa caaatgagtc ctattatctt
    45061 ctcaataaac ctgaaaaagc tgttacagct gtatcagatc cagaaaagac gacagtaatc
    45121 gatctgattg caagtgccga tcgtttccaa ccattatatc cagtgggaag actagataga
    45181 gatacaacag gactattgct gatcacttcg aatggacaat tgggatatga aatgctactt
    45241 ccagaaaaaa aggtttcaaa gacttatcaa gtagttgtga acgaaagggt gacaccggca
    45301 gacgtaacag catttgaaaa tggaatcgta ttcgctgatg ggtatcaatg taagccggct
    45361 gatttaaaaa tactcaaagc agacaagcac caaagtacgg ttttattgac gatccaagaa
    45421 ggaaaattcc atcaagtcaa aaagatgttc ttagcccaag gaaagaaagt gaccgcattg
    45481 aaacggctaa gcatgggacc attggaatta gatgaacagc taggagtagg cgagtatcga
    45541 tcgttgacat tagcagagat gaaaaagtta agtatttatt ttaaatagga ggtcaaaaag
    45601 atgaaatata aaactttatt gtttgatgta gatgatacat tgttagattt tcaattaact
    45661 gaagccaatg ctttaagtgc tttgtttgca gaacaaggaa tcacattgac accagaaatc
    45721 gaaaccagct ataaggcttt gaatcatcat ttatggcagg agtttgaaaa aggcagacta
    45781 tcacgagaag aaataatagg cagtcgtttt gggttacttt tccagcaatt tgggaaaaca
    45841 gtagatagtg atcaaatgga aaaacgctac cgccactact tgaaccaagg tcatgatctt
    45901 gttccaggaa gtctagaaat cctgaaaaag gtccatccgc atgctgatct ttatattgtg
    45961 acgaacggtg tgtcgcacac gcaacatcgg cgattaacag atgcaggaat gcttgattac
    46021 ttcaaggata tctttgtctc agaagctgtc ggagcgcaaa agccgatgaa agaatttttt
    46081 gatcatgtct tttcccacat tcctaatttt gataaaaagc aaactgtgat cattggtgat
    46141 tctttgactt ctgatatcaa aggaggaaat atggcaggga tcgatacgat ttggttcaat
    46201 agaaataaat taccagaaat cgaagaaatc cagcctactt atcgaatcga ttcattagag
    46261 gaactttatt cattattgga aattgaaaag gattaatttt cagaaaaata tagcattttc
    46321 gtgttttttc gctataatag attagaatga aaaaacggat acgaaagagg ttttagtcaa
    46381 tgacaaaaag caagccaatt atcattggtg tgacgggtgg atccggaagc ggaaagacaa
    46441 gtgtcagccg tgcgattttc aacaacttcc cggaccattc catcatgatg cttgaacaag
    46501 attcttatta caaggatcag agccatttga gttttgaaga acgattgaac acaaattacg
    46561 atcacccatt tgcctttgat aatgatctgc tgattcagca tgtaggcgac ttactgaact
    46621 ataaagccat tgaaaaaccc gtgtatgact atgtagcgca tacaagaagt caggcaacga
    46681 tcattcagga accaaaagaa gtgatcattc tagaaggaat cctgatttta gaagacgaac
    46741 gtctgcgtga cttgatggat atcaaagtat acgtagatac agatgatgac attcgtatca
    46801 ttcgtcgtat caaacgggat atggaagaac ggggacgcac attggactcc gtgatcgaac
    46861 aatatttgac agttgttaaa ccgatgtatc atcaatttat cgaaccaacg aagcgctatg
    46921 cggatatcat tgttccagaa ggcggagaaa accacgttgc tattgatttg attacaacaa
    46981 aagtagctag tttcttaaac cataaatagt gcgcagcgaa acagttgaac ggactgcgac
    47041 cgtttgaaaa gcaaggattc tgctttttga acagtcacca gccaactgtt tttttattta
    47101 taacaagagg ttgtgaaaac agcatcttga cctaagtatt aaaaagaaga aacggaaaat
    47161 agctgtttat attttaccgt tttttgagct aataccgaag gtcagctgat tgagcaccgt
    47221 gaagtagagg ttgtaaaaaa gctgtctttg catcaagcga taagagcatc caaataaaac
    47281 agctcctcac gtttttcgtt gggttgggct tattatcgta gatgcaagct tttgaacacc
    47341 gtttaacgag aggttgtaac agaagtggtc agatgcaata aataagaaga aacttacgaa
    47401 aattatttct cgaatttgtg tgagatttcg acttattttt ttgaagaagc tcttttgtcg
    47461 caccccc
//
LOCUS       DAEGIQ010000011        45401 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000011 DAEGIQ010000000
VERSION     DAEGIQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.011"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            259..1449
                     /locus_tag="KMT45_000549"
     CDS             259..1449
                     /locus_tag="KMT45_000549"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBE7004036.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            1551..2504
                     /locus_tag="KMT45_000550"
     CDS             1551..2504
                     /locus_tag="KMT45_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBE7004037.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            2573..3922
                     /locus_tag="KMT45_000551"
     CDS             2573..3922
                     /locus_tag="KMT45_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7004038.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(4105..4428)
                     /locus_tag="KMT45_000552"
     CDS             complement(4105..4428)
                     /locus_tag="KMT45_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004039.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            4557..5720
                     /locus_tag="KMT45_000553"
     CDS             4557..5720
                     /locus_tag="KMT45_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBE7004040.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            5790..7139
                     /locus_tag="KMT45_000554"
     CDS             5790..7139
                     /locus_tag="KMT45_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBE7004041.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7184..7615)
                     /locus_tag="KMT45_000555"
     CDS             complement(7184..7615)
                     /locus_tag="KMT45_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7004042.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            8003..10645
                     /gene="alaS"
                     /locus_tag="KMT45_000556"
     CDS             8003..10645
                     /gene="alaS"
                     /locus_tag="KMT45_000556"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBE7004043.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            10845..11441
                     /locus_tag="KMT45_000557"
     CDS             10845..11441
                     /locus_tag="KMT45_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBE7004044.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            11563..12711
                     /locus_tag="KMT45_000558"
     CDS             11563..12711
                     /locus_tag="KMT45_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBE7004045.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(12749..13162)
                     /locus_tag="KMT45_000559"
     CDS             complement(12749..13162)
                     /locus_tag="KMT45_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBE7004046.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            13241..13672
                     /locus_tag="KMT45_000560"
     CDS             13241..13672
                     /locus_tag="KMT45_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBE7004047.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(13771..14004)
                     /locus_tag="KMT45_000561"
     CDS             complement(13771..14004)
                     /locus_tag="KMT45_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBE7004048.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            14187..15857
                     /locus_tag="KMT45_000562"
     CDS             14187..15857
                     /locus_tag="KMT45_000562"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBE7004049.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16004..16390
                     /locus_tag="KMT45_000563"
     CDS             16004..16390
                     /locus_tag="KMT45_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004050.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            16377..17075
                     /locus_tag="KMT45_000564"
     CDS             16377..17075
                     /locus_tag="KMT45_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBE7004051.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            17080..17544
                     /locus_tag="KMT45_000565"
     CDS             17080..17544
                     /locus_tag="KMT45_000565"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBE7004052.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLERKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            17541..18452
                     /locus_tag="KMT45_000566"
     CDS             17541..18452
                     /locus_tag="KMT45_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBE7004053.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            18687..19226
                     /gene="pyrR"
                     /locus_tag="KMT45_000567"
     CDS             18687..19226
                     /gene="pyrR"
                     /locus_tag="KMT45_000567"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBE7004054.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            19240..20523
                     /locus_tag="KMT45_000568"
     CDS             19240..20523
                     /locus_tag="KMT45_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBE7004055.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            20536..21462
                     /locus_tag="KMT45_000569"
     CDS             20536..21462
                     /locus_tag="KMT45_000569"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBE7004056.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            21481..22761
                     /locus_tag="KMT45_000570"
     CDS             21481..22761
                     /locus_tag="KMT45_000570"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBE7004057.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            22766..23842
                     /gene="carA"
                     /locus_tag="KMT45_000571"
     CDS             22766..23842
                     /gene="carA"
                     /locus_tag="KMT45_000571"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBE7004058.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            23847..27032
                     /gene="carB"
                     /locus_tag="KMT45_000572"
     CDS             23847..27032
                     /gene="carB"
                     /locus_tag="KMT45_000572"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBE7004059.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            27086..27862
                     /locus_tag="KMT45_000573"
     CDS             27086..27862
                     /locus_tag="KMT45_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBE7004060.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            27859..28791
                     /locus_tag="KMT45_000574"
     CDS             27859..28791
                     /locus_tag="KMT45_000574"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBE7004061.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            28788..29504
                     /gene="pyrF"
                     /locus_tag="KMT45_000575"
     CDS             28788..29504
                     /gene="pyrF"
                     /locus_tag="KMT45_000575"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBE7004062.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            29511..30140
                     /locus_tag="KMT45_000576"
     CDS             29511..30140
                     /locus_tag="KMT45_000576"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBE7004063.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            30128..30442
                     /locus_tag="KMT45_000577"
     CDS             30128..30442
                     /locus_tag="KMT45_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004064.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            30480..31121
                     /locus_tag="KMT45_000578"
     CDS             30480..31121
                     /locus_tag="KMT45_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBE7004065.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            31164..31856
                     /locus_tag="KMT45_000579"
     CDS             31164..31856
                     /locus_tag="KMT45_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBE7004066.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            31853..32755
                     /locus_tag="KMT45_000580"
     CDS             31853..32755
                     /locus_tag="KMT45_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE7004067.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(32913..34619)
                     /gene="efbA"
                     /locus_tag="KMT45_000581"
     CDS             complement(32913..34619)
                     /gene="efbA"
                     /locus_tag="KMT45_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBE7004068.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            34809..35432
                     /locus_tag="KMT45_000582"
     CDS             34809..35432
                     /locus_tag="KMT45_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004069.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            35882..36931
                     /locus_tag="KMT45_000583"
     CDS             35882..36931
                     /locus_tag="KMT45_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE7004070.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            36928..37809
                     /locus_tag="KMT45_000584"
     CDS             36928..37809
                     /locus_tag="KMT45_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004071.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            37812..38582
                     /locus_tag="KMT45_000585"
     CDS             37812..38582
                     /locus_tag="KMT45_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7004072.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            38861..39715
                     /gene="aroE"
                     /locus_tag="KMT45_000586"
     CDS             38861..39715
                     /gene="aroE"
                     /locus_tag="KMT45_000586"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBE7004073.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANSIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            39893..41056
                     /locus_tag="KMT45_000587"
     CDS             39893..41056
                     /locus_tag="KMT45_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBE7004074.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            41173..41583
                     /locus_tag="KMT45_000588"
     CDS             41173..41583
                     /locus_tag="KMT45_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE7004075.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            41757..43985
                     /gene="topB"
                     /locus_tag="KMT45_000589"
     CDS             41757..43985
                     /gene="topB"
                     /locus_tag="KMT45_000589"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBE7004076.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            44156..44440
                     /locus_tag="KMT45_000590"
     CDS             44156..44440
                     /locus_tag="KMT45_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004077.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            44600..44836
                     /locus_tag="KMT45_000591"
     CDS             44600..44836
                     /locus_tag="KMT45_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE7004078.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            44833..45231
                     /locus_tag="KMT45_000592"
     CDS             44833..45231
                     /locus_tag="KMT45_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBE7004079.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT        14895 a         7871 c         9858 g        12777 t
ORIGIN      
        1 acgaaagggc ttctatcaat ggttgtgttg tatttatctg cccaggtaga gtaaaataga
       61 tcattatcta aaaggtaatt gttcatacta tataagtcat ttgaaagtaa aaagatactg
      121 taaataaaaa atgtggttaa ataattctca taatcagtgt aagcgttatc aaaaatatag
      181 catacttgct ttgtagatga atgacacgat atatgtttgg tatgttctgc aagacagatg
      241 aatatggagg tggagacaat gtcttccaaa gaactaacaa tgtatttaga gaaagaattg
      301 gatcaactga aagaaaaggg gttatacaat acgatcgaca tactagaaag tgaaaatggt
      361 gcttgtatca tcgtagacgg aaaaaagatg atcaatctag catccaacaa ttatcttgga
      421 tttgccaatc gcgaagaatt gaaaaaagca tgtattgaag caaccgaaac ttatggtgta
      481 ggagcaggag cagtccgaac gatcaatggt tcgctgaaaa tccatcagca attagaagaa
      541 aaaatcgcag aattcaaagg gacagaagca gcaatcgcct ttcaatctgg attcaactgc
      601 aatatgggcg ctatctctgc agtaatgaca aaagaagatg ctatcttatc ggatgaatta
      661 aaccatgcat ctattattga cggttgccgc ttatccggag caaaaatcat ccgaattaaa
      721 catcaagata tgaaagacct tgagaaaaaa gcaaaggaag cgatagaaag taagaaatat
      781 aaaaaaatca tgtatatcac agatggtgtt ttctccatgg atggagacat tgcacgattg
      841 ccggaaatta ttccgattgt tgaaaaatat ggactgatca cctacgtcga tgatgcgcac
      901 ggctcaggcg taactggaaa aggtgcagga accgtcaagc attttggatt aagcgacaaa
      961 atcgatatgc aaatgggcac gttatcgaaa gctataggtg ttgttggggg ttatgtagct
     1021 ggatcgaaaa cactaatcga ctggttgaaa gctagaagtc ggccattcct attttctact
     1081 tcgctaactc caggtgcagc tgcagctgca ttggcttcga ttactttgat gcaagaacat
     1141 ccggaacttg tcgaaaaagt ttgggaaaat gctaattatt ttaaagaaga attgaaaaaa
     1201 gtaggctata acatcggtat gtctgaaaca cctattactc cagtcatttt gggagacgaa
     1261 aaagtgactc agactttctc taagaagctg atcgaacatg ggatttatgc taaaccaatc
     1321 gtttatccaa ctgtgcctct tgggacaggc aggatcagaa atatgccgac tgcggaacat
     1381 acgagggaaa tgcttgatga agcagtagca gtttatcaaa aaataggaaa agaaatggaa
     1441 attatttaac taaatttagt tttttcgtct atttaaaatt agagaacatg ttcacgcata
     1501 tattgttaat catctgaaga tagtagtaaa aaagatcagg gaggatcatc atgaaaaaag
     1561 tattggtgac aggatgttta ggacaaattg gttcagagtt gatcacacgg ctacgtcaag
     1621 aactaggcag tggaaatgtc attgcgacag atatacgcat gcctgaagtc attgagaaaa
     1681 acgatatctt tgagatccta gatgtaatgg attacgaaaa aatgcgcagc atagcagaac
     1741 aatatcaagt ggacacattg attcatttgg ctgctctttt gtcagctgtt gcagaagcaa
     1801 aaccacaatt agcctggaat ttgaacgtga ctgggctggt caatgcattg gaagtcgcta
     1861 gagaattcca gctgaaattt tttacgccaa gttcaatcgg cgcgtttggt ccaaatactc
     1921 caaaagatca taccccacaa gatactcttc agcgtcctac aaccatgtat ggggtaacga
     1981 aggtagctgg agaactacta tgtgattatt attatgtgaa atatggcgta gatactcgtg
     2041 gtgtccgttt ccctggattg atttcttatg caaccttgcc aggaggcggt acgacagact
     2101 acgcagtcga tatttattat tcagcaatca aagaaggaaa gtacactagt ttcattgatc
     2161 aaggtacatc aatggatatg atgtatatgc cagatgcgat cgatgccatt ataaaactca
     2221 tgaatgcaga tcctgatcga ttggttcatc gaaatgcctt caatatatcg gcgatgtctt
     2281 ttgacccgga agagatcaaa gcatctattc aaaaaatcat accagatttt gaaatggatt
     2341 atgatgtgga tcctgtacgt cagtcaatag cagaatcttg gccaaattca ctagatgttt
     2401 cttgtgcgca agaggaatgg ggcttttcgc cgaaatacga cttagatgtg atgacaaagg
     2461 atatgttgga aaaactgaaa gaaaaattgg tagagacggt ataaaacaag aaccacatcc
     2521 aaaaagtttt ttatactttt gggattggct atggtatgaa aggggacaag ggatggaaga
     2581 atatcaagcc acgccagtaa aagaagtaaa aatagaaaat gaattaaaac gtacgatggg
     2641 attttttact gctttatcga ctgttatggg cacagtgatc ggtgcagggg tattttttaa
     2701 agccgcaagc gtagcagaag ttacaggatc cgccagtttg catatgtttt cttggtttct
     2761 cggtggtatg atttctgtct gtgcaggact aacaggagct gaactggcag ctgctattcc
     2821 agaaacagga gggatgatta aatatatcga acggatttac ggaaatacag cagctttttt
     2881 attgggctgg gcgcaagtcg tgatttattt tccagcaaat gtagcagcct tgtcgatcat
     2941 tttcggtaca cagttcgtca atctatttgg tctttcccag tcaatgattg ttcccgtagc
     3001 tgttacagct gctgtatcga tcctgttgat caatttttta ggttctaaag ctggaggggc
     3061 ttttcagtcg attacgcttg tctgcaagct tattcctttg tttgtgatcg tcattttcgg
     3121 tttgttccga caagaaggag tagacttcca actgtttccg atccaagcag gagaaaatct
     3181 ttcatttttc tcagccttgg gagcgggctt gctagcaacg atgttcgcat acgacggttg
     3241 gatacacgta ggaaatattt cgggagaatt gaaaaagccg gcgaaagatt tgccaaaagc
     3301 aatttcttta ggaatcatcg gaatcatgat cgtttattta ttagtaaatg cggtgttctt
     3361 aagaaccgca tctatcgacg gagtcgcagg aaacagcaat gcggcaagtg acgttgcaaa
     3421 aatgattttt ggcggcttcg gtggaagact ggtcacagtt ggtatcttga tttctgttta
     3481 tggaaccatc aatggctata cccttactgg aatgcgtctc ccttatgtta tggcaaaaga
     3541 aaacaacctg ccttttagca agctctttgc taaacttcat gataaaacta aagttcctgt
     3601 agccgcaggt atcttagagc tagtcatcgc tattggcatg atgatgatcg gtggttttga
     3661 tacactaacg gatatgctga tctttgtcat ttggattttt tatacgatgg tctttgttgg
     3721 tgtcattctt cttcgtaaaa aagaacctga tttgtttcga ccatacaaag ttccaatgta
     3781 tccgtttatt ccactagtag caatcattgg agggactttt atcgtatcaa gcacgcttat
     3841 cactcagacc tttttagctt cgatggggat agtattgaca cttgctggta tcccgattta
     3901 tctctattta aaacgtagat agctaaaaca ttcgaatttt attagaactg cacttctctg
     3961 ccaaacaaaa tattttattt aaatgaggag tacgtggaag aaatgtcaaa agtactcgtt
     4021 aattttgatc acgtaaatgt cgttttgtct acgatgaaat agcaaagtaa aagacagaaa
     4081 tcgggctcaa ccgatttctg tcttttactt tgatttgcgt aactgaacta ttcggcgtac
     4141 gccgcgataa atatttaata aaaccaagca accagaaatg aaaatgggaa gccaaatcag
     4201 atagacaggg tagccaagca ctttagacaa agcatgtgga gtcattttat ccatttcaag
     4261 aagtgcttgc tgccaagtct gaagattgaa gactagcatg aaacccgaaa taatcaggag
     4321 aataaatcct aaataatgaa ttgtagttct ttttcttaca taagcgatcg tagcataaag
     4381 gaacagtaaa aaaataggta cgccaaaaac aaagtacata ttcagcatct cgacaccccc
     4441 tttgtgttta tcttacttta ctttgaaaga aatgagaaat aatagcctcg ttacttgctt
     4501 gaaacagcaa aggactgcta agataagaga gatacaaaga atcaaggagg agtcatatgg
     4561 caagttatat cgaaccgatt cgttttgcgc tgattgtttt tccattttta gccttagcga
     4621 tctcatctgt tttttttatt tatgaatatc gaaagtatgg aacatttcta gtaacaaggg
     4681 cagttatttt gtattctttc gttttttatt tattgtgtgc gtattttttg gtgatcttac
     4741 cacttccggc aaaggagacg gttgcccaaa tgacagggcc aaagatggag ctgcatttat
     4801 ttgcttcatg gcaacatttc agagacaaca cggtattggt cttgtctgaa cctagtacct
     4861 acctgccggc aatgaagcag agtgtatttt tggaaccagc ttttaatgtt ttattactca
     4921 taccatttgg catttattta agatattatt tccggctttc ttttatgaaa acgatgattg
     4981 ctagtttctg cctttctcta ttttttgaat tgacgcagct aagtggactt tattttatct
     5041 acccaagacc ataccgctta tttgatgtga atgatttagt gacaaataca ttgggtggaa
     5101 tcattggttt tgtcgttacg ccgttattta catttatgct tcctacaaga agacgaatgg
     5161 atgaactttc ttatgaaaaa ggaaagactg tctcattgac tcgacgcttg gtcgcttatt
     5221 tgatcgactg gacattttta tctcttatta atttggtagc ggcgattttc tttaaaattg
     5281 cgttcgattc aggggcagtt acttctcata tctggtggct tttattagaa gtaggggtat
     5341 actttatgat tgtaccgttt ataaccaatg gatggacact tggcaaaaaa attgtgcgta
     5401 tccgtttggt ggaagaagac cgcgaacgaa tttctttaaa agcattgatg atccgctacg
     5461 ggtatctgta ctttcttttt tatggtttaa gctggttggg gcagttgctt cagcaaatca
     5521 tgaattcgac aacgaatacg gtgttgcaga tgagtgcagt attcatttct ctcggtgtct
     5581 tgttactaca aggtattttt attttaaata tcttgctatc atttttgaga aaaaaacgga
     5641 ttctgtttta tgaaaaagct agtcacacat atactgttag cacgattggc caaacaaagg
     5701 atgatgattc tcgaaattag ctgagcaaaa acgctttgtc tgtgttataa tgccaaagtc
     5761 gtgataattt aaagagaaga ggtgcacgaa tgaataattt tgatatattc cgcttcaaac
     5821 cgtttatcaa tgaagcgtta accgcaaagg gcttcaaaca gcccacagag gtccaggaac
     5881 gtttgattcc tttaattgca aaaggaaaaa atatcattgg gcaatctcaa actggtacag
     5941 gaaaaacaca tacgtttttg ttgcctttgt ttgaaaaaat caatgcagaa aaagaggaag
     6001 tacaagttgt tattacagct ccaagtaggg aactagctgc acagatctat gaagcagcta
     6061 cacaaatcgc taaattttct acaccggaga tccgtgtctc gaattttgtc ggcggtacag
     6121 acaaacagcg tcaattaaat cgtctgcatc accaacagcc acaggttgta gtaggcacgc
     6181 ctggtagaat attagacatg atgaatgaac aagcgctgaa gattcacacc gcatttgcat
     6241 ttgttgtcga tgaagcggat atgacattgg atatgggttt tttaaatgaa gtagaccaaa
     6301 ttgcaagtcg attaccagaa aagctgcagt ttttagtttt ttctgccact attccagaaa
     6361 aacttcgtcc attcttacga aaatacatgg aaaatccaat cattgaagaa atcaaaccaa
     6421 aagcagttat ttctgaagac attgaaaatt ggctgatttc aacaaaaggt aaagatgaga
     6481 atcaaatgat ttatcaactg ctgacaattg gtcatcctta tttagcaatc gtatttgcca
     6541 atacaaaaca gcatgtggat gaaattgccg attacttgaa aaaccaaggc ttgaaagtag
     6601 caaagattca tggagacatc accccaagag agagaaaacg agtgatgcga caagtacaga
     6661 atctggagta tcaatatgtt gtagcgactg atttagctgc tagagggatc gatatcgaag
     6721 gtgtctctca tgttattaat gcagaaattc cagaagatct agacttcttt atccatcgtg
     6781 tcggacgtac tggacgtaat ggcttaaaag gaacggccat tacgttatat tcgccaaatg
     6841 atgagcaagc cattgatgcg atagaaaaat tgggcgtctc atttgaacca aaagaaatca
     6901 aaaacgggga aatcgtcaaa acatacgatc gtaatagacg tgccaaacga gaaaaatcaa
     6961 aagatgtatt agatcccacg ctgattggtc ttgtgaagaa gaaaaagaaa aagatcaagc
     7021 ctggttataa gaaaaaaatc gattgggcaa ttgcagaaaa aaataaaaaa gaacgcaaaa
     7081 tcgaacgcag acagcaaagc cgtactgctc gtaaaaataa gaaaaattca cataaataaa
     7141 aaacaagagg ttgtgacaaa aggcgtgtca caatctcttg tttttaagct agactttttt
     7201 tcattccgat acaaagtact tcatcttcaa taaaaggttc tgtgcaagta tgataccctt
     7261 gtttttcata aaaatctaca gccgtcattt cagctactac ataactagtc tgacaacctt
     7321 ctgaaaccgc ccgtttttca gcagcttcaa gcaactgacc gcctaaccct tttcctcggt
     7381 attcttttgc tacgcaaaat cgttccggct tgaagcaagt aggtgttttc ttttcataac
     7441 gcaaagtagc tactggtctt ttttgatcaa gaaatagata ataggtttgt tgcgggatat
     7501 cttttccgtc gaattctagt tctgcaggaa tcccttgttc taatacaaaa acttgatatc
     7561 tcaaaaagca gctagctgcg cgtatccatt catccgctcc aactattttt ttcatcatat
     7621 tcctccttgt catgaatagc agagatgtat tgattccttt attcttatcg taccaaaaat
     7681 cgtggagtat gaccaaaagg aaatgaaaat aaaaattgag atattgacag tgatcaatag
     7741 aatgcgtata ctaaataagt acgaaaagga tatgatttgt tttttaagac tcacagaaaa
     7801 ttctttcttg gctgaaaaaa gaatagtcac taatcaaatt gctccctttt tctattttgg
     7861 tagaaatacc gacgcagctc agcgttatcg agtttaagag gtaatgatta cacatcattg
     7921 caatcaaggt ggtaccgcga gttttcgtcc ttggctgtga tggtgttttt ttgtatttaa
     7981 tttatagaaa gcaggtaatg acatgaaaca attaagtagt gcacaagttc gacaaatgtt
     8041 tcttgatttc ttccaatcta aagaacatac gattgaacct agtgcgtctt tgatccccgt
     8101 gaacgatcca accttattgt ggatcaactc tggtgttgca acattgaaaa aatattttga
     8161 tggttcagtt gtgccagaaa atccaagaat cacaaatgcc caaaaatcta ttcgaacaaa
     8221 tgatatcgaa aatgtcggta aaacagcacg gcaccatacg atgtttgaga tgctgggtaa
     8281 tttttcgatt ggtgactatt tcaaaaatga agccatccac tgggcatggg aatttttgac
     8341 tgatccaaaa tggatggcat tcgatccaga aaaactttat gtaacagttt atccaaaaga
     8401 tacagaagca aaacgcatct ggcatgaaga agttggcctg ccactagatc acattattga
     8461 tgtagaagac aacttctggg atatcggtgc aggcccatgt ggtccggata cggaaatttt
     8521 ctatgaccgt ggagaagctt ataacgattt agcagaagac gatcctgaaa actatccagg
     8581 aggagaaaat gaacgttatt tagaaatctg gaacttagta ttttcagaat tcaaccataa
     8641 gcctgataac acatttgaac cacttcctca taaaaacatt gatacgggga tgggattgga
     8701 acgcatggtc tcaatcgttc aagatgcgtc aacgaacttt gagacagact tgtttatgcc
     8761 tattatccac gccgtagaaa agctaagcaa acaagtaaaa tatggtgaaa acccagtaac
     8821 agatatttct tacaaagtaa tcgcagatca tattcgcgcc ctttcatttg ctattggcga
     8881 cggtgcattg ccatcgaatg aagggagagg gtatgtctta cgccgtttgc ttcgtcgtgc
     8941 tgtcatgcat gggaaaaaat taggtattga acaagcattc ttatatcgtt tagtccctgt
     9001 tgttggggaa atcatggtaa gttattatcc tgaagtactt caacaaaaag aatttattga
     9061 aaaagttatc cgtacagaag aagaacgttt caatgaaaca atcaatgaag gtttggaaat
     9121 cttaaatcaa gtgatccaag aagtaaaaaa cgaaaacggg acgactctag caggaaaaga
     9181 catcttcaaa ctatacgaca catatggctt cccggtagaa ttaacggaag aggtggcaga
     9241 agatgaagga ctgaaagttg atcatgctgg ttttgaaaaa gaaatggatg cacaacgcga
     9301 acgtgctcgc tctgcccgca gtactgaaaa atcaatgggg gtacaatctg ctttattgac
     9361 agatatcaaa gtagaaagta aattcattgg atacacagaa caagaatcag atagtcaatt
     9421 attggtcatc gttaaaggtg agtctctttt gaatgaagtc gcagaaggtg aagctcaaat
     9481 gatctttgat caaacacctt tctatgcaga aatgggtggg caagtagcgg atcaaggatg
     9541 gatcatgaat gaagtaggcg aaatcgtagc agttgttaca gatgttcaaa aagcgcctaa
     9601 tggtcaattt atgcacaccg tagaagtaaa agcaccaatg agtgaaggac aaacctatca
     9661 tctattgatc gatcaatcga tgcgcaatcg gatcatcaaa aaccatacag caacccatct
     9721 actgcacaag gcattgaaag aagtacttgg cgagcatgca aatcaagctg gttctttagt
     9781 cgctcctgga catttacgat tcgactttac ccactttggt caaatcactt cagatgaatt
     9841 agctgaaatg gaaagaatcg tcaacgaaaa aatctgggaa gctatcccag ttgaaacaat
     9901 tgaaacagat atcgatacag ccaaaaacat gggtgcaatg gctttgtttg gtgaaaaata
     9961 cggacatgac gttcgagtag taaatattgg cggctggtca atcgaactgt gcggaggcac
    10021 acatgtagca aatacagaaa acatcggaat cttcaaaatc gtttctgaat caggaattgg
    10081 tgcaggcgtg cgacgaatcg aggctgtaac aagtaaagaa gcgtacgaac tacttgaaga
    10141 agaagaaaaa caactaaaac aagtagcaac aattgttaaa tcacctcaat tgaaagaagt
    10201 tgtttcaaaa gcggaacaat tgcaacagca agtaagagag ctacaaaagg aaaatgaaca
    10261 attagctagc aagttagcga atcaacaagc tggtgatgta ttcaaagatc cagaaacgat
    10321 caatggaaaa acaatcattg cagcacaagt aaaagtgaaa gatatgaatc agctgcgtca
    10381 attagctgac caatggaaac aaaaaaatat ttcagatatt cttgttttag caaacgaaca
    10441 agaaggaaaa gtcagtctgc tagcggcaat gacgaaagaa tcaaatgaag ctggactaaa
    10501 agctggcgac ttgattaaag caatcgcacc gaaagtaggc ggtggcggtg gtggacgtcc
    10561 agacatggca caagctggcg ggaaaaatcc tgctggtatt ccagatgcac ttaaagctgc
    10621 gaaagaatgg gttgcgcaac aatagggcgt ttacgtttga cagaaaaaca aaagttcaaa
    10681 cattgaacga aacatgatta tgggggcgaa gacaaacaag tcttgccctc atactttttt
    10741 caattggatg gcgcatctaa tcaaatggtc ggattcggat ggtctttagt tgacgaaagt
    10801 gacagaaatt tttacgatta gtataaataa aaaggggtga aaaaatgaat agtagaaata
    10861 agacctttcg actagtctta cgtgcgatct tgttagcaat catcatcgta caagcgatgg
    10921 ttccttggct ggggtttatt ccattaggat tcattagttt aacaattatc catatcacag
    10981 taattattgc tgcagtagta ttaggcccaa aagatggcat ggtgattggc ttgttttggg
    11041 gaatcgctac aattgttcgt gcctatgcca tgccaacaac gccatttgat acattagtgt
    11101 tcacgaatcc gattatctcg gtagttccca gagtacttgt tggcttagta gcaggcttgg
    11161 ttttccactg gatttatcaa aaaaataaat caataacgat cagctcagcc tttgcagggg
    11221 tactgggttc attggtcaat acagtcttag tgttaggatt catgggatta ttttatacag
    11281 gtgcaacggc agatgcctat ggtgtagact ctgctctttt atttaaaacc ttagcagggg
    11341 ttgcagcaat caatgggata ccagaagcaa taggtgcggg aatcataaca ccattactag
    11401 caaaagcgtt atttgcagca acaccgttga aaccagaata atgaccatct tttaatagta
    11461 ataggacgca cgatagatag aaaaaggaag agaggagagg aaaacaatcg tcctctccat
    11521 ctttttacct gagcaacaag caaactagaa ggggtaaaaa acttggctaa gaaacaagaa
    11581 gaaaaaaaga aacaaatacc gcaacagcgg acaaaaaaag gagtactagc gggtatctta
    11641 ggattggtta cgaatctgat cttgtttgtc tctaagctaa tgatcgggct tttttcagga
    11701 agtgtctcca ttatggctga tgcagtgaac agtctatcag atactgcttc atccatattg
    11761 actttgatcg gatttaaaat tgctgcaaag ccagcagatc aggaacatcc ttatggacac
    11821 gaacgcttcg aatatatcag tgggttattt gtttcgatca tcattactta tgtaggattt
    11881 caatttttag agtcttctgc tgaaaaaatc atccatccag aaaatgtctc tttaacaccc
    11941 atcgtctttt ttgtcctaat catttccatc tttttgaaat ttgcacaagg caaaatgtat
    12001 caggttattg cgcaatccat tgattctgaa acattgagag ctacagcaac agatagttat
    12061 aatgatgtct ttactacact ggctgtattg ctttcagcag caatcgaatg ggtaactgga
    12121 tggcgtattg atggatatat aggttttgct ttagcattat atatccttta tagtggattg
    12181 acgatgcttc gaaattttgt atacgaacta ttaggaagtc gcccaacgcc agcagagatt
    12241 gaagaaatgg aagagcattt aaatgcttgc gatgatatac ttggctatca tgatttattg
    12301 gtgcataact atggacccaa taaacgcttt gcatcagtcc acatcgaagt agatgaatca
    12361 atgaatttga atgtggctca taagattatt gatctgatag aaaaagactt taaaaagtct
    12421 ttggatgtgg aacttgtctg tcacttggat ccaatgcctg ttgataacga aaaatactat
    12481 aatatcttgc ggcagttaaa gcaaattctt cttgaaacag gtacagggct taatattcat
    12541 gattttagag taattcatca aggaaaaacg ctgcaatttg atgtagtggt ccctgagaaa
    12601 ttccagtttg ttgatacaga actagaaaga atcttacgta gtcaaataga agaaaaaatc
    12661 ggagattacc aattggaaat tacgtttgat cacaattatc ttttatatta aatgagaaac
    12721 tataaaaaga ctccttaaag tttttaattt aaagagtctt tttttctttt aattgtttat
    12781 ggagagcctg acgtgcaaga ttatctgctc ctttattttt gttttcgggc aaccattgca
    12841 ggatcaataa agaaaattcg gatagaagtt cttggattct ttgaaggtag ggaaggaaga
    12901 gctcgttctt cgttgcgttt ttgtcgaccg tagaaactag tgttttgctg tctgaaaaac
    12961 agaaaatcgt ttcattctgt aagtcgtttt tctttagaaa ttctaaagtt tttaaaaaga
    13021 cttcgaactc tgcttgatgg tttgtcccta tagaaagcgg gatagatagc tgttcttgtt
    13081 taccttgata gacaatcaag attccacctc cactaggacc agggttgcct tttgtagagg
    13141 catcaatgta tacttttaac ataaaaagag attctccttt ttgttcaaac taatgttatg
    13201 gtaagattat atcattactg aatcggtgag aggttagaga atgaaaaaaa atattcgatg
    13261 gcagcctgaa ctggcacata cgatcatcta ttggtcattg accttgatca ttctgttttt
    13321 tagtctgatt ctttctttag aaaacacagg tccttattgg aaaagcaatt ttgttttagg
    13381 tatttttctt gtttttctat ttctaggttt gcggcgttct tttattttaa aatcaaactc
    13441 tgtgattata cgttatgctg ctttttggaa agaccgagaa atatctgtcg gacagattcg
    13501 atctttgacg ccaactaata ctggggtgca aatgatgttg caagatgaga aagccccatc
    13561 tgttttcatg atgcgaaaaa aagatcaagt agtccttttt gatcattttg aaaaaaacgg
    13621 acccaaatca gaatcaattc cttatcctta tacgaaaaaa agaaaaagct aattttaaaa
    13681 atcagaacct aaaaaaacga atttttacag gaaaagaaaa gagtgagacg tacggttcat
    13741 cccgtttagc gtctcactct atttgtttat ctattcatgc atactagtat cggtagtctg
    13801 aacgttgcct ttcttgacat gggcagcttg tgttcctcga ttcccttcga tgatctcaaa
    13861 ttcgaccact tgattttctt ccaaagtttt gaatccctct tcttcaatcg cagtaaaatg
    13921 tacaaaaatt tcttctgtgt catcataact gataaagccg tatccttttt tgttgtcaaa
    13981 ccattttaca attcctgtgg tcaaagcact cacgaccttt ctaaagtctg atcatagcct
    14041 tcattataaa gagtatttat aagtacgtca aataaattaa aaacctgtaa ttttatgttt
    14101 tttttctgaa taaaaaaagg tataattggc atatcttaac aaagaagata ttttgttgaa
    14161 taaagttcgt gaaaaggggt aatcaaatga agaatgatct gcaaatttca caagaaacag
    14221 agctagaacc gattgttgaa gtggctagta aattagatct taaggaagac gatttggaac
    14281 tgtatgggaa atataaggcg aaaatcaatt tttctggaat caatcgatca aaggataacg
    14341 ctgaggggca tttgatctta gttacttcca tcaatccaac accagctggc gaaggaaagt
    14401 caacgatcac tgtagggtta ggagacgcgc tcaataaaat caataaaaaa tctgttattg
    14461 cattgcgaga accctcactt ggtcctgtga tgggaatcaa aggtggtgct gcaggcggag
    14521 gatatgcaca ggtattgcct atggaagata tcaacttgca ttttactggt gacatgcatg
    14581 ctatcacaac agctaataat gcattatctg ctcttttaga taatcatatt catcaaggaa
    14641 atgaattgaa catcgatgcc cgccgagtga tttggaaacg ggtagtcgat ctcaatgatc
    14701 gtgaactgcg taaagtaatt gttggcttgg gtgggccaat ccaaggtgtt ccaagagaag
    14761 atgggtttga tatcacagtt gccagcgaaa tcatggctat cttgtgtctt gcggcagacc
    14821 ttgaagattt aaaggctcgt cttgcaagga tcgttgtcgg ctatacattt gaccgtcagc
    14881 ctgtgactgc tggagacttg aaagcagaag gcgctctggc attactgctg aaagatgcaa
    14941 tcaagccgaa tctagtacaa acaatttacg gaacaccagc ttttgtccac gggggaccgt
    15001 ttgccaatat cgctcatggc tgcaatagta ttttagctac caagacagca ttgagattag
    15061 cagattacgt tgtcacagag gcaggatttg gtgcagatct tggcggagag aaatttttag
    15121 atatcaaagt acctaacttg aaaaaagcgc cggatgctgt ggtgattgtc gccactgtcc
    15181 gtgcattgaa aatgcatggc ggaatgaaaa aagatgaact taaaaatgag aatcttgatg
    15241 ctttgaaagt aggttttgca aatttaaagc gccatatacg taatatggaa caatatcagc
    15301 tacctgtgat cgtcgcaatc aatgaattcg taacagatac agacaatgaa ctcacactgt
    15361 tggagcactt gtgtgaaaat caggggattc ttgccaagag agcgagtgta tgggcagatg
    15421 gagcagaagg cggcgttgat ttagcaaaag ctgtagtacg attgattgat agaaaagaag
    15481 ctaattacaa gccattatac cgcctagaag aaacgataca agaaaagaca gaaacaatcg
    15541 tcaagaaaat ttacggagga aatggtgttg ttttcagcaa aaaagcgcaa aaacaaatag
    15601 aagagtttac gaagaatgga tgggacaaac tgccgatttg tatggcaaaa acacagtatt
    15661 cattttctga tgatccttca ctgctaggtg caccggaaga tttcaccatc acgatccgcg
    15721 aaatcatccc aaaattaggg gcaggatttt tagtagcctt gactggagat gttatgacga
    15781 tgcctggttt gcctaaaaaa cctgctgcat tgaatatgga cgtgacaaat gacggagaag
    15841 tgttgggatt gttttaagct tgattataat ctaccatcga aggagctgta ttttgactac
    15901 agcttctttt tttagacgat agggaattaa ttttgtttac actaggatca ttctttttct
    15961 ttattgtatt ttgagctata ctaaagcata ataggagggt atgatgaaaa cgacatttat
    16021 aagaaaaacc ggtttttacg gtatgggtag ctcgattcag ctaagaatca acaatgaaaa
    16081 acgcagtgtt ctcagtcatg aacaaaagat aacgcttgat ctggaagtgc cctttacgat
    16141 gcaggtcacc ttttattggc taaaaagccc cgtttataca gtgaaagaag caaagcctgc
    16201 ttatgtgatc acaatgaatt acttgattct tcaaatctat ccgtttttat ttctagcaac
    16261 tgggttgggg gccgttttcc tacaaaattt atttttcagt ttttttgcag ttattgtgtt
    16321 gattgcgttc tttttctata taaaaaaccg ggcatatttg atcaaggagg aaaattatgg
    16381 caaactctga tcttttttta atcagtttca atcgaatcga aaaatggatg agagacgaga
    16441 tgggaaatgc acgaaatatg ggatttaccg aacttgtcag acgcctagct caaagaaaac
    16501 aattaatgat cagaaaatac gaagatgatc ttttacagct tgctcagtta aggaatgcga
    16561 tcgtccatga tcggatcgca gtcgattttg tcattgcaga gccaaatgat tggacagtca
    16621 agcgaatcca gctgatcgaa caagaactga ttcagcctga aaccgtgctg cctcgatttg
    16681 caaaacgtgt aacaggtttt gaacaagatc ttccgttact tgaactgtta aagatagtag
    16741 cagaaaaaag gtattcacaa tttccgttat acaataaagg aaaatttgta gcattgatta
    16801 ctttacgtaa tatcgggttt tggctagcaa aagaaagcca aaaaggtccg gttgatttaa
    16861 caaataaacg agcgttggat ttgatcatcc aaaacggaaa gtacacaaat tatcattttg
    16921 ttccagctaa aacacatatt tacgaggttg aagcgatgtt tcgtgagcaa gggactttgg
    16981 aagccgtact tatcactaaa gatgggaatc ccgatggtaa tctgctcggg atcgtacgac
    17041 ctcgtgatat ttataaacaa atagaaaagg attgattttt tgttaattat ttattggata
    17101 ttcagcgctt tgctagtagg agtggatcag ttggtcaaat ggtggatcac tgataatcta
    17161 gcgcttggag agacgaaaaa tcttatacca ggtattttgt cactgaatca tattcgaaac
    17221 actggagccg catggagtat gctagaaagg aagatgtggt tctttactat agtgacactc
    17281 attgcagtcg ttgtgattct cacattgatg attaaaaatc gtgaaaaagg taatcgctgg
    17341 ttcatgattg gattgagttt gattctagct ggtgcgattg gcaattttat cgaccgtatt
    17401 cgtcttggct atgtagtaga tatgttccag acagatttta tgagctttcc gatttttaac
    17461 gtagcagatg tgacacttgt atgtggagtg atttgtatgt taatttatat tattttagat
    17521 gaaaaggatc aaagaaaaaa atgacagaac ttaaaacgac gattcaccat gaatctggta
    17581 ggatcgataa agtattgact aatttattca ccgattacag tcgttcgcaa attcagctgt
    17641 ggctgaaaaa tggagcggtt tctgtcaatg gacagccagt caaagcaaat tacaaagtaa
    17701 aacaaggaga cgagattact gtcttgatcc ccgaaccaga aacattagat gtggtcccag
    17761 aaaatatccc gttgacgatc gtttacgaag atgaagatgt agcggtgata aacaaacctc
    17821 aaggaatggt cgttcatcct tcagcaggtc atacgcatgg cacaatggtc aatgcgctga
    17881 tgtaccatat caaggatctt tctacgatca acggcgttat tcgtccaggt attgttcacc
    17941 gaatcgacaa agatacttcc ggactgctaa tggttgccaa aaatgaccag gcacacgaat
    18001 cattagctca gcaattaaaa gataagacct ctttgcgcaa atatgttgct ttagtccatg
    18061 gagagattcc tcacgaaaaa ggaaccatca atgcgccaat cggccgttcg aaggaaaacc
    18121 ggaaaatgca ggcgataaga gaagatggga agtcggctgt cacacatttt actgtattgg
    18181 aacgtttcga aggatttact ctggttgaat taaaactaga aaccggaaga acacaccaga
    18241 tccgtgtgca tatgaagtat atcggttatc cgttagctgg agatccagta tacggaccga
    18301 agaaaacttt gaaaggcaat ggtcagtttc tccatgcgca aatgttaggt tttacgcacc
    18361 cgactacaaa cgaaaagatg atctttgaag cacctcttcc agaagttttt gaaaaaactt
    18421 tagaaaaatt aagaaaaaac attgcatttt aatttagata acggtatagt aattaacaac
    18481 gaaagcataa aacttaatag aattccttta agtttagtcc cgtgaggcta agaagggtgg
    18541 gagcaaggat acgtatggtg cgccataagt agattgctca ttgacatagt atagctatca
    18601 gtgtatcagt atacgctcag tctaaacatt cctcctgtcc tcttagggca ggagtttttt
    18661 tatgcaaaaa agtaggggga atgaaaatgc aagcaaaaga agtagtagat caggtaacca
    18721 tgaagcgagc attgactcga attacttatg agattatcga gagaaaccat agtatccaag
    18781 atatcgtatt ggtaggtatc aaaacacgag gaatctacat tgcatcaaga attgccgaac
    18841 gcctgaagca gctagaagat atcgatattc ctgtaggaga actggatatc actttatacc
    18901 gagatgacaa aaaagaaaat ccagaagaac cggaacttca ttcttctgac atcccagttt
    18961 ccttagaagg aaaagaagtt attttgattg atgatgtttt gtatacagga cgaacgattc
    19021 gagcagcaat ggacgcagta atggattttg gcagaccaag aaaaatttca ttagcagtcc
    19081 tggtcgatcg tgggcatcga gaattgccaa tccgtgcaga ttatgttggg aagaacattc
    19141 caacatctcg ggcggaagag attttagtag agatgcaaga gcttgatgga caagatcgca
    19201 tcatgatttt gaaagaggaa gattagagga gagagaaaca tgtcggagaa aaaaggattt
    19261 cataatgatg acgtggtgtt agatatccac gatcgcccaa ccccaggaca ttgggtagga
    19321 ttaagtttac aacacttgtt cacgatgttc ggagcaaccg tgttagtccc catcttagta
    19381 ggtatcgatc caggtattgc gctagtcagt tcaggactag gtacattggt gtacttattt
    19441 actactaaag gaaagatacc tgcgtactta ggaagcagct tcgctttcat tgcagcgatg
    19501 cagcttttga tgaaaacaga tggttatcca gcgattgcac aaggagcagt cacaactgga
    19561 ttagtctatt tgattgttgc gctgatcatc aaaaagatcg gttcagcatg gctggataaa
    19621 atcttgcctc caatcgtcgt cggtccagtc atcatggtta tcgggttggg acttgcggca
    19681 aatgcagcaa caaatgctat gtataatcag ggcacgtatg atttcaagta tattgctgta
    19741 gcgctgatca cattaggatt aacaattttc ttcaacatgt ggctaagagg ttttctcgga
    19801 ttgattccga ttttactagg tattattagc ggatacatcg tagccttgct gtttggaatc
    19861 gtggatacta agccaatcgt agacgctgcc tggtttgctg ttcctaactt tgagattccc
    19921 tttgtacaat atgaaccaaa gctatatctt ggagcaatca caacaatggc acccatcgct
    19981 tttgtgacga tgacagagca tatcggccat cttatggtat tgaataaact aactaaacgt
    20041 aatttctttg aagaaccggg tcttcataag acgttgacag gtgatggatt agcacaaatc
    20101 gtggcagcct ttgtcggagg tcctcctgta acaagttacg gtgaaaacat tggtgttctg
    20161 gctatcacac gtgtccatag cgtgtttgtg atcggtggag ctgcagtatt tgcagtcatt
    20221 ttaggattcg taggaaaatt aagcgctttg attttaagta tccctggacc agtcatctca
    20281 ggaatcagtt ttatcttatt tggagtaatt gccgcaagtg gaatgaagat tttaatcgag
    20341 aacaaaatcg attttgataa aaagaaaaat ctgttgattg cttcagttat cttagtagta
    20401 ggaattggcg gactcgtctt tgaagtaggc acatttacat tgtcggcaat ggcattagca
    20461 actgtacttg gcatcatttt aaatcttatc ttaccagaaa cttcacgaag cgaagaaaac
    20521 taaggaggaa acattatgat catcaaatca gaacgtatca gcttaaagca tttattgacc
    20581 gttgaggcac ttactgatca agaagttatg ggcttgatcc gcagagggca ggaattcaag
    20641 caaggtgcta attggactcc gcaaaagcgc cagtactttg ctacaaacct ctttttcgaa
    20701 aatagtactc gcacacataa aagcttcgat gtagcggaga aaaaactagg gctagaagtg
    20761 attgaatttg aagcaagcac aagctctgtc caaaaagggg aaacactata cgatacagta
    20821 ctgacgatgt cagcattagg cgtagacgta gcggttatcc gtcatggtga tgagaactat
    20881 tatgacgaac tgatacaaag taaaacaatc cagtgttcga ttatcaatgg aggagacgga
    20941 agcggacagc atccaaccca atgcttgctt gatttgatga ccatttatga agaatttggt
    21001 cattttgaag gtctgaaagt tgccatcgta ggtgatatca ctcactctcg cgtagctaaa
    21061 tccaatatgc agatgttaaa acgtctaggc gcacaagtat tcttctcagg tcctagagaa
    21121 tggtacgacg atgaattcga agtgtacgga cattatatgc cattggatga actactagat
    21181 caagttgatg tcatgatgat gcttcgggtg caacacgaac gccatgatgg aaaagaaagt
    21241 ttctcaaaag aaggctacca tcaggaacat gggttaacag ttgagcgtgc gaagaagttg
    21301 caaaaacatg caatcattat gcacccagca ccagttaacc gtgatgtaga attagctgat
    21361 tcattagtgg aaggattaca gtcgcggatc gttcagcaaa tgagcaatgg agtattcgtc
    21421 agaatggcaa tcttagaagc agtgctaaat ggaaaagcgt aattatagga ggatgaaaaa
    21481 atgaaaacac tcattaaaaa cggtcagatc aatacacgaa aaaatatgac gacaccagca
    21541 gaaatttgga tagaagatgg gcgaatcaaa gcaatcggta caggattctc agaagccgaa
    21601 tttgatgaag tgtttgatgc aaagggacaa ttgatcacac ctggtttagt ggacgtccat
    21661 gttcatttga gagagccagg atttacatac aaggagacga tcgaagctgg gactcgctca
    21721 gctgctcgtg gcggtttcac tactgtatgc gcgatgccga atttaaatcc tgtacctgat
    21781 acggcagaaa agctgagaca agtatatgac atcattagaa aagatgcagt tgtgaaggta
    21841 ttgcagtatg cgccaatcac agaaaatctg cgaagtgaga aattagttga tcaagaagca
    21901 ctgattgaag aaggtgcttt tgctttcaca aatgacggtg taggtgtcca aacagctggg
    21961 acgatgtatc tggctatgaa agaagctgca aaaaacaata aagccctagt agcccataca
    22021 gaagatgaat ctttattatt tggcggcgtt atgcacgctg gcaagaaggc agaagaactt
    22081 ggtcttcccg ggatcttaag cgtaaccgag tcttctcaaa ttgctagaga tctgttgcta
    22141 gctgaagcaa ctggtgttca ttaccatgtt tgccatgttt ctactaaaga aagtgtccgt
    22201 gtaatcagag acgcaaaaaa agcaggaatc cacgtaacag cagaagtttc accccatcat
    22261 cttattttga tcgacgaaga tattcccgaa gatttcggct tttggaaaat gaacccgccg
    22321 ctaagaggac gggaggacag agaagctttg atcgaaggct tgcttgacgg tacgatcgat
    22381 tgcatcgcta cagaccatgc cccccatgga ttagaagaaa aaagccaaag tttcatgaaa
    22441 tcaccatttg gtatcgtagg aagcgaaaca gcgtttcaac tgatctatac tcattttgta
    22501 gaaactggtc gattcacgtt ggaacaagtg atcaattggt tggcagttaa accggctgag
    22561 atttttggtt tgaatgcagg aacgctaacg gttggtgccc ccgctgatgt agcagttttt
    22621 gatattacac aaacttgcac gatcgataaa gaagactttt tatcaaaagg ggagaataca
    22681 ccttttatcg gctggaaagt aaaaggcgaa acacaaatga cttttgtcaa cggaaaactt
    22741 gtgtggcaga agggagaata aaagtatgga acgtttgctt atattagaag atggaacagt
    22801 tttcgaagga aaggcttttg gtgcagatgc tagtgtcttc ggagaaatcg tttttacaac
    22861 aagtatgact ggttatcaag aaactatcac tgatcaaagt tttaacggtc aaatcattac
    22921 atttacctat cctatggtgg gtaattatgg ggtaaataga gatgattatg aatcgatttc
    22981 tccaacatgc aaaggagtag tagtaaaaga acatgcacgt gtagcttcaa actggcgaaa
    23041 ccaaatgacg ctagattcat ttttgaaaca aaaaggaatt cctggaatat caggtatcga
    23101 tacgcgcgcc cttactcgca aaatccgtga agtcggaacg atgaaagcta gtatagttga
    23161 tgctggagat tccttcgaac atgcatttga tcagctgaaa gcaaccgttc tagcaaccaa
    23221 tcaagtgcag caagtctcaa ctaacaaacc ttatccaagt ccaggaacag gtcggaaagt
    23281 cattgtcatt gacttcggct tgaaacatag tattttacga gaattgagta aaagaaactg
    23341 taacctcaca gtacttccct ataacacaga tgccgagacg attttagcgc tatcaccaga
    23401 cggagtcatg ctgacgaatg gacctggtga tccaaaagat gtgccagaag caatcgaaat
    23461 gatcaaaaaa atccaaggaa aagtaccgat ttttggtatt tgtctcggtc accagttatt
    23521 tgctttagcc aacggtgcag atacctataa aatgaaattc ggacatcgag gattaaatca
    23581 cccggtacga gaagtagcga ccggaagaat tgattttact tctcaaaatc atggctatgc
    23641 agtcgatgaa gccacgattg atccagaaaa actcatcgta actcatgtag aagtaaatga
    23701 cggaacagta gaaggagtca aacacagaga ttatcccgct ttcagtgtgc agtttcatcc
    23761 agatgctgcc cctggtccgc atgatgcact acatttattt gatgaattta tggaaatgat
    23821 ggacgcagga aaggagcagt aaacagatgc cgaaacgtac agatatcaag aaaattatgg
    23881 tgattggatc tggtccgatc atcattggtc aagccgcaga atttgactac gcaggaacac
    23941 aagcctgcct tgccttaaaa gaagaaggat atgaagtggt tttagtaaat tctaatcctg
    24001 cgacgatcat gacggataaa gaagtggcag atcaagtata tatcgaaccg atcacacttg
    24061 aatttgtatc aaggattctt cgaaaagagc gtccagatgc tctccttcca actttaggcg
    24121 gacagacagg cctcaatatg gcaatggagc tggcagcatc aggtatttta gaagaattga
    24181 acatagaact tctaggaaca aagctttctg cgatcgatca agcagaagat cgtgatctat
    24241 tcaaacagtt gatgaaagaa ttgaatcagc caatccccga gtctgaaatc gtcaatacag
    24301 tagaagaagc tgtagatttt gcaaataaaa tcggctatcc tgtcatcgtt cgtccagcct
    24361 ttactttagg cggaactggt ggaggtatgt gtgaaaatga agaagaatta cgtatcgtat
    24421 cagaaaatgg attaagtctt tcccccgcta cccaatgttt gattgaacga agtattgcag
    24481 gttttaaaga aattgaatat gaagtgatga gagattcggc tgataatgca atcgttgtct
    24541 gcaatatgga aaattttgac ccggtgggta tccatacagg tgattctatc gtatttgccc
    24601 ctagtcaaac attgtctgac catgaatatc aaatgcttcg tgatgcttct ctttcgatta
    24661 ttcgagcact taagatcgag ggaggctgta atgtccaact cgctcttgat ccacatagtt
    24721 ttaattatta tgtgattgaa gtcaatccgc gtgtttctcg ttcatctgcg ttagcaagta
    24781 aggcgactgg ctatccgatc gctaagctag ctgctaaaat agcgcttgga ttaacattag
    24841 acgaaatgaa aaatcctgtt acaggaacga cttttgcaga atttgaacct gcattagatt
    24901 atgttgtagc taaaattcct cgctggccgt ttgataagtt tgaaaacgga gaacggcttt
    24961 taggcacgca gatgaaagca actggtgaag tgatggctat cggtcggaat atcgaagagt
    25021 ctttattgaa agctgtgcgt tctttagaaa tcggtacaca ccatatcgaa cttcctgagt
    25081 tggcagacat cacggacgat gacctaatcc aaaaagtggt tcgagcacaa gatgatcgcc
    25141 tgttttatgt agctgaagct attagaagag ggtacaccat agaagaatta gcggatttga
    25201 caaaaatcga tttattcttt ttagataaac tgttgcatat cgtagaaatc gaacagcagt
    25261 tgaaacagca gccgttggat gacaagatat tgaaaacagc gaaacaaaac ggttttacag
    25321 acaaaaaagt ggcggagctg tgggaaataa acgaagaagc cgttcgccag atgagaaaag
    25381 aaaatgattt gattcctgta tataagatgg ttgacacttg tgcagcggaa ttcgaatcaa
    25441 aaacacctta tttttatagt tcctatgaat atgaaaatga aagttcacga tcagaaaagc
    25501 cctcaattct agtactggga tctggtccta tccgtatcgg acaaggggtt gaatttgact
    25561 atgcgactgt gcattctgtc aaagcaatcc agcaggcagg atacgaagca atcatcatga
    25621 acagcaatcc tgaaacagtt tcgactgact tttctatttc ggataaatta tattttgagc
    25681 cattgacctt cgaagatgtg atgaatgtga tcgagctaga acagccgata ggagtaatcg
    25741 ttcaatttgg tggtcagaca gcaatcaact tagccgaacc tttgactgct gctggtgtaa
    25801 aaatcttagg gacaacgatc gaagacttgg atcgtgcgga aaatcgtgat ttattcgagc
    25861 aagcgctgaa agagttggat attcctcaac ctttagggga tactgctaca agtaaagaag
    25921 aagccgtggt tatcgcttcg agaattggtt atccagttct tgttcgtcca agctatgtat
    25981 taggcggacg tgcgatggaa atcgttgaaa accagcgaga tttagaagat tacatggaac
    26041 atgctgtgaa agcatctcca gaacatccag tactagtgga ccgttacttg cttggtggtg
    26101 aatgcgaagt ggatgcgatt tgtgacggtg aaacagtact catcccaggg atcatggagc
    26161 atattgagcg tgctggtgtc cattcagggg attcaatggc tgtgtatcca cctcaaacat
    26221 tatctgaatc gatcaagaaa acgatcgaag attacacgat ccgcttagca ttaggtttga
    26281 attgtgtcgg gatgatgaat atccagtttg tcatccatga aaatgaggtt tatgtgatcg
    26341 aagtcaatcc tcgagcaagt cggactgttc cattcttaag taaggtgaca ggaattccaa
    26401 tggcacaaat tgcgacgaaa gctattttgg gagagaaact aaagacactt ggttatgaaa
    26461 acggacttta tccagaaaca aaagaagttc atgttaaagc acctgtcttt tcttttacga
    26521 aattgcagaa agttgatact tacttagggc ctgaaatgaa atcgactgga gaagtaatgg
    26581 gatctgacag acaattagac aaagctttgt acaaggcatt cgaagcttct ggattacact
    26641 tgcctgaata tggagccgta ttatttacga ttgcggacga aacaaaagaa gaagcactag
    26701 aattagctaa acgttttaca gaaattggct atagtctgat aggaacgaaa catacagctg
    26761 cttatttcga aaaaaatggt ctggtcgttg aaacagtagc gaaaatttct gaggaagcag
    26821 cagaaaaaaa tgtagtcgac ttaataagag atggtaaaac acaagtggtt gtcaatacga
    26881 ttgataaaga tcggggaaat gcttctaaag acggtttcat catccgaaga gaagcagttg
    26941 aacatggtat tccattgttt acttctttag acacagcaga tgcgatcgtt cgagtgatgg
    27001 aatcccgtgc tttttcaact aaccctattt aacggcgagc acatgttaag acaaaaagac
    27061 gaaaaaccgt tgcaggaggg gacagatgaa acaagaaatg atggaaatcg ttcagcaaaa
    27121 acagctggca ccaaagattt ttgaaatgac attgaaaggt gatctagtaa aagaaatgca
    27181 gcaaccagga cagttcttgc atattctcgt gccgcgtgca gacttgctat tacgccgacc
    27241 gattagcttg aatcatatcg accacgaaac aaatacttgc cgtatcattt atcgggtgga
    27301 aggtgagggg actagagtct tctctactct ttctgccgga gaccatttag atgtcatggg
    27361 tcctttaggc aatggttttg atttgtccaa tcttcataca ggagacgaag tatttataat
    27421 cggtggagga atcggtgtcc ctcctctata tgagttatca aaacagttag tcgaaaaagg
    27481 aattaagcca acgcattttt taggttttgc cacacatgaa gtcgtttatt atgaaaaaga
    27541 atttaatcgt ctaggagaaa caagaatcgc tactgacgat ggaacatacg gtatgcacgg
    27601 aaatgtcggt aatttattgt taggaacgaa gacggaacct acagctgttt ttgcatgtgg
    27661 ttcaaacggg ttattgaaga cagtggaaca gctgttttct tcgcatcaaa atgtccagtt
    27721 atcattagaa tcccgcatgg catgtgggat aggggcttgc tatgcttgcg tttgtcatac
    27781 gccggaagat gaaacaggaa caaaaagtgt gaaagtttgt gacgaaggac cgatttttaa
    27841 aataggagag gtaattatat gaatgagaat cctttagcgg tcaaacttcc tggcctagaa
    27901 ttaaaaaatc ctatcatgcc ggcaagcggc tgtttcggtt ttggcaaaga atacggaaaa
    27961 tactatgatt tgaatcagct tggatcgatc atggtcaagg caacgactcc aaatgcccgc
    28021 tttggcaatc ccacgccaag agtggcggaa acaccatctg gcatgttgaa tgcaattggt
    28081 ttgcaaaatc ctggtctaga cgttgttatg cataaacttc ttccagaatt agaggagtac
    28141 aatgagttac ccatcatcgc aaatgttgct ggtgcttgtg aagaagacta tgtagaagtt
    28201 tgtagcaaaa ttggcgaagc accaaatgtg aaagcaatcg agctgaacat ttcttgccca
    28261 aatgtcaaac atggcggaat tgcttttgga acagattctg atgttgcttt tcaattaaca
    28321 caagcagtaa agaaagtttc ttctgttcct gtctatgtca aattgtcccc aaatgtaaca
    28381 gacatcgttc caatcgctca ggcaatcgaa gctggagggg cagatgggtt tacgatgatc
    28441 aatactcttc ttggtatgcg gattgacctt aagacaagaa aaccgatttt agccaatcaa
    28501 acaggtggac tctccggacc agcaatcaag cctgtcgcta ttcgtttgat tcaccaagta
    28561 gcaagtatat ccagccttcc aatcattggg atgggcggcg tgcagacagt ggatgatgtt
    28621 cttgagatgt ttatggctgg agcaagtgct gtagctatag ggaccgcgaa tttcaccgat
    28681 ccgtatatct gtcctaaact aattaaagag ctccctgtgc gaatgagcga actaggcatc
    28741 gaatcgctgg aaagattaat caaagaagtg agagaggaac gtgaacgatg aatcaactag
    28801 accaacgacc aattattgca ttagattttt caactaggca agaggtagaa gactttcttc
    28861 gatttttccc gaaagaagaa aaattgtttg tgaaaatcgg aatggaattg ttctaccaag
    28921 aaggacctga aatcgtacgt tatctaaaag aggctggtca taatgtattt ttagatctga
    28981 aacttcacga tattccgaat acggtcgaaa aagcaatgcg gggattggca aagcttggtg
    29041 ttgatctcac ctgtgttcat gccgctggcg gtattcgtat gatggaggcg gcaatgcgag
    29101 gattagaaga ggggactcca gaaggtggaa aacgtccgct acttctagct atcactcagc
    29161 ttacttctac aagtgaagaa gaaatgcatg ccgatcaact aatcgaagtt tctttagaaa
    29221 aaagtgtgat ccattatgct agctgtgcca aaaaagcagg attagatggt gtggtttctt
    29281 ctgcttggga agtagaagcg atcaaagaaa tggcaggaga cgaatttgtt tgcttaactc
    29341 cgggtataag accagaagga acagtcgcag gagatcagac aagagtagtc acaccttctc
    29401 aggcaaaaaa gatcggctca acttttatcg ttataggtcg accaatcact cagtcaacta
    29461 atccttataa agcatatcaa acgattcaaa cagaatggag ctaacctaaa atgaatgttg
    29521 aacaaagtat cgcaaaagac ttattagaaa ttgaagcagt atttttaaat ccaagtaatc
    29581 cttttacatg ggcaagtgga atcaaaagcc caatttactg tgataaccga atcacgatga
    29641 gctatccaaa agtccgaaaa gagatcgcaa aaggattagc aagcaaaatc aaagaagctt
    29701 accctgaagt acaagtgatt gctggaactg caacagcagg aatccctcat gccgcatggg
    29761 tagcggaaat actggatttg ccaatggtct atattcgaag taaggcgaaa gatcatggga
    29821 aaggtaacca aatcgaagga cgtatatttg aaggtcagaa gatggttgtg atcgaggatt
    29881 tgatttctac aggcggaagt gtcttggaag cagctgaagc agcaaaaaga gaaggtgcgg
    29941 atatccttgg tgttgcggct atctttacgt atgagttgcc aaaaggaaaa gcaaatttcg
    30001 aaaaagccga aatccctctt atgacgttga ccaattattc tgtattgatt gaagcagctt
    30061 tagaagatcg ttatatcgat gagcaagaat taactctgct aaaagaatgg aaaaaagatc
    30121 ctgaaaattg gcagatttaa gcgcgcttga tgaagataga gaaatccgat ttgtgtctcc
    30181 agaatcatca attggaatag caaaaatcca aaaactgatt gcgagaagca tcttttcatc
    30241 aggtatgaaa agaaacttct atcgtgatca gtttttgatt tttttaaaat tcatttttct
    30301 aagactggtt agcagaaaga agagaataat ctcttctctt ttttctatta tatatagatt
    30361 ttctctattt tttggtaagg gagctgtttc aaaaatggta aagagcggaa atgttgcgaa
    30421 tatcagttat ttaatgatat gatattcgga aaaagaaatc tcaaagggag gtaactttta
    30481 tggggttgaa tgaatttatt ttaggattga acaatttaga gacgatcaca agggcacctg
    30541 gattttttaa atatacagag catacagttg cagcacattc gtatcgtgta gcatctattg
    30601 cgcaagttct aggagacatt gaagaagcga atggacaacc gatcgattgg aaactgctct
    30661 atgaaaaatc attgaatcat gactacacag agcgttttat tggtgatatc aaaacgccgg
    30721 tgaaatacgc aagcaaagag ttaagaggga tgctgcaaac agttgaagaa aagatgacgg
    30781 acgaattcat caaccaagag attccagctg agttccaaga agtttatcgc cgtcggttgt
    30841 tcgaaggaaa agatgaaacc gtagaaggag aactattagc catttcagat aaggttgacc
    30901 ttctttatga atcttttgat gaaatcatca aaaataaccc ggaaaaagtt tataaggaga
    30961 tgttcattga ggcagtgact acgattaaaa gctttgaaca tcggccttct gttcagtatt
    31021 tctttaaaga gatatttccg gaactattga accaggaatt ttatggaaaa gaggcgtttt
    31081 tagaaaccgt ctcaacgatt gtaaagaaag aagaaaaata aaaatgatat gagatgcagg
    31141 gggaagtata gttgaaaaag aatatggatg tcgaatgggg ctattctgga gacaaaggtc
    31201 cggaaaattg ggcgagttta tgtgattggt ttgcgagagg tgcggaattt ccattgcagt
    31261 cgcctattca tttgaccaag ggggaagaaa ccaaaataga aggagacacg ctttcctttc
    31321 attatcaaaa gcaacggttt actgacaagg aattcaaaaa cacgattcat ttagtacctt
    31381 ttgaccaact cagttatgta gagttcaaga aagaacgcta ttatttgaca gatattcatt
    31441 tccacttgcc ttctgaacat atcatcaacg gaaaacagga agatattgaa tttcattttg
    31501 ttcatatgaa cagcaaaaaa gagaatctag ttgtgggtgt catgtaccag ctaatggtac
    31561 aggaaggttg gctgtataat caagaaaatg gggaaagttg gaacttagaa aaacatgagc
    31621 attgggtaaa tccggatgtg ttctttccgg agcaaaaaag ccatttccat tatattggct
    31681 cattgacaac tccgcctacc agcgggccaa tccaatggtt tgtaatggat catgtaggaa
    31741 aactgaacca agagtttctt ctccccttcg atggtcagta tgctcgaccg aacaaccgac
    31801 caatccagcc cttgaatgga agagaaattt attatttcga ggagtttgag caatgaatat
    31861 tcagcagatg aaatacgtgg tcgctgtagc aaacaatggt agtttcagag aagcggcaaa
    31921 gaaaatgttt gtctcccagc caagtctttc agctggtatc aaagaattag aaaccgagtt
    31981 agggatcacg ctgtttactc gaacgaatcg aggggcatat ttaacagaag aagggcaaga
    32041 cttcttaaat cgtgccgaga aaatattggt ccagcttgat acattagaaa ctcactatct
    32101 aacgagtgaa aagagagaca atttttcgat cgctgcccag cattatgatt tcttaggtcc
    32161 tttgaccgca cagctactcg aggaattcaa agacgagatc aaacaatttc ggatcatgga
    32221 gacgacaaca gcgagagtga tcgaagaagt caaagagcag cacagtgaga tcggcattat
    32281 ttatatgaat gagcataatc aagctggtat cagaagatat ttacaacaag gagagctaag
    32341 tggccgcagc attggaaaat tcaaaacgca tattttttta aggcaaggac atccattagc
    32401 tgaacacgca gagatcacca aagaagaatt gttatcttat cctcaagttc gttttacaca
    32461 ggatggaaat aatttcccgt atttttatga agaccttatc gagatccctg atcaagaaac
    32521 agtgatttat acgagtgatc gaggtacttt gatgaatatc gttcttgaaa ctgatgctta
    32581 cgcttcagga tctggtattg tgattggagg gattcgcgag cacttgcggt taatcccttt
    32641 ggcagaaggc gaactaaatg aattttatat catttattcg gcgaaacgtc cgctcagtga
    32701 aatcgctcaa agatttatca aagagcttac tcggttactg gaccagcaga gctaagaaaa
    32761 gcagatgaga acgctgttta ttgaacaccg ttgatcaaaa aagaggggtt gtgacagaat
    32821 tttgtcacaa ctcctctttt tgtagcaaac aatttataac taattgctgt tgtgggaaag
    32881 tacgcaaata tgattctaga aacactcatt gattacctgt tttgtttcat agctgtaatt
    32941 ttttcttctt ctggtgtcac gataatcgtc ttttggtttt cataaataac ataaccaggc
    33001 ttggcgccat ttggttttcg gatatgcttt acctgtacaa gatcaaccgg cacttgggca
    33061 gagtatcggt attttgaaaa ataggcagct aactcggcag cttctgtaat tgtttcatct
    33121 gagggcttgt cacttttaat gatgacgtgc gaaccgggta tattttttgc atgaagccaa
    33181 tagtcggttt ttttagctgt cttcattgtc agctggtcat tttgaagatt gtttttacca
    33241 acaagaatga gcgttccgtc agaagaaaag tattgatcag gctgagatgg tttcttacgt
    33301 ttttgttttt ttaagttctt ctttttcagg tatccttctg aagtcaattc ttctttaatt
    33361 gcttcgatat ccattgggcc ggcaatttcc agttgagata agacagattc aagatattgg
    33421 atttcatctt tggcttcttg gatttgttcg ccaatcagct taacagcgtt cttcagcttt
    33481 tggtatcgat ggaaatattt ctgtgcattt tgatttggtg tcaatgccgg atctagtgct
    33541 attttgattg gccgatcttc ttcataataa ttaggtaaaa caacttcttt tgcgccgcgt
    33601 ggtacttgtg tcagaaaagt agtaagtagt tcgccatttc tacggtaatt ctcagcgttt
    33661 tcggattctt tgagtgtttg ttcacgtttt ttcaatttat ttttatttcg ttttagttca
    33721 ttttcgattt ttcgaatcag ttctccgcct tgttgttttg cccgatcttt ctctgctttt
    33781 tcgtgataat agctatctaa taattgactg agtgttggat aagctgttac agcagatgcc
    33841 tgttcggaca atacctgata agcaatcggt gtgaaaaaat ctttattttc cgtttcgtaa
    33901 aaagtaggaa taggctgatg gctgatggct gagaaaaatt cgtcccatac aaccatcttt
    33961 tcattaggac gttccagtaa tcttttggtc agctcttgag cgatatcgaa accgattccc
    34021 tgaaactgac gttggattgc ttttggatct agttcagttt gggaaagccg ctgaaagatt
    34081 ttttctttct cggagctaaa tgggttcaag acctcttgtt ttggcggagc aacatattct
    34141 acaccaggaa gcaaagaacg ataagtgttt tgagagcttc caatgtgttt gatcgcatcc
    34201 agtatttttc cagtttctcg attaactaag acgatcgtac tatgacgtcc cattaattca
    34261 acgatcaata tgatattttg tagatcaccc aactcgtttc gtttagcaaa gtggaaatgg
    34321 atcactcgat cattttcgat ttgttcgatg tcttctaaaa tagcgccatc caaatatttt
    34381 cgaagcatca tcacgaagtt aggcggatta tcaggattct gataatcgat ttgggtaatc
    34441 tgtacacgag cataacttgg gtgcgcagat aataataatc gatggttttt gccgcgtgaa
    34501 cgaatcacca gcacgatttc attttcataa ggctgatgga ttttcgagat ccggccagat
    34561 agtaacgttt cacgcaattc gttgaccatc gcgtgggtaa atacaccatc aaaggacatg
    34621 gttcattcct catttctttt cataagtata ttcattataa cgaaaaaaag aagaaaaacc
    34681 aatttggccg aatagtaaag caaaagtcta gaaaaatctt ggactaatta cataaaaaac
    34741 agtgtttcta tcgttgaaat ccaaattttt aggtataata aaaaatagga gaaaaagtag
    34801 aaaagaaggt gtttgttatt tgggaagtag ttaaatcgag agtacaagaa atgaatgaac
    34861 atttctcagt gtcattcgca gaaaatccaa gtaagtattt tcgacgtctt gtttttctgc
    34921 tcatcttatc attgctgtta acagttgcaa tgtttttatt gccggaaaaa atccgagaag
    34981 agcaagtaaa tagtctcgtt tattcgtcca taccaaccag caaattgaaa ccttttgatt
    35041 acagcaaagc tgatcagaaa atcaaacaaa gccgggcgat atcggtgatg ttcagcaaac
    35101 cgaacgggaa aacctatcag gacatgcttg atgtttttaa tgatcctgag cttatggaag
    35161 aattgaatcg accgatcttt tattatccga tcgtttatga tgtacatgaa ctggaacaaa
    35221 aatataacat acgaacggat gaagtgacat ttattttctt tgatggagga aaagaagcaa
    35281 atcggataac tgcgggtaag ggaggaataa ctgattttga taaggagttg atccctgaac
    35341 tgaatcgact tccgttagcc aatatcaaac aattagaaga agaattggat agaacgggag
    35401 taacttccca aaattccgag attgctaatt gagacattgg cgcgatgaaa cggtgggtct
    35461 atatagcgct atttttaatt atcagaacaa gtgatgtttg atcttcaaaa taacaaataa
    35521 aaaattaaaa aatatagttg acgaaattca aaaattcgat tagacttaaa tcaattctta
    35581 aggaaagggt gacctgtaat gaacttatca aaacgtattt cagcagaaaa caaaaaaaat
    35641 tggcgcagct ggcgtagata agttgtatta atggtgagac tttcccatag atacaacttt
    35701 ttagagtgca tttctaaagc ttgtatctat gctagacatg aacattgact gccgcataga
    35761 ttagaatcta tgcggttttt tgtgtaaaga caatgaccgc tttggccatt gtcttttttt
    35821 gtagactgga tacttgcctg attataataa ataaaaagat tgactttgca ggaggaaaaa
    35881 aatgaaaaac aaacgattaa taacagttgt agtaattatt ttagtttatt tagcaggtgc
    35941 atttatttat ggaaaagcct caggcgaaaa agaaagtact tcgacgaacc agacaaaaaa
    36001 aacaacagtt gttggggtat tgcaatacgt gagccatccg gcattagatg agatttatcg
    36061 aggaatcaaa gatggtctga aagaagaagg attaaaagaa ggagaaaatc ttgagatcaa
    36121 atttcaaaat ggacaagccg atcagagcaa gctagccaca atgagtcaac agctagtaca
    36181 atctgatcca gatgccttga ttggaatcgc aacacctgcc gctcaatcat tagcaaacac
    36241 gacttcatcg atcccagttg tgttaggtgc tgtaacggat cctgtggggg ctggattagt
    36301 cgcttcttta gagaaacctg gtggtaatgt aacaggtgtt tctgatcagc cgcctgttac
    36361 ttcacaaatc aagcttgctt ctgatttgtt gccaaatgca aaaaaagtcg gaatcttgta
    36421 tgcttcttca gaggataatt caaaatacca agtagcacaa gcagaagaag ctgcaaaaaa
    36481 agaagggtta gaatcagtaa aatatgcggt gccttccact aatgagattg cacaaacggt
    36541 tcaagtgatg agccgacaag tcgattttat ctatgttcca ttagataata ccattgccaa
    36601 tgcgatgcaa acagtggtaa aagaagcaaa caaagcaaat atccccgtca ttccatctgt
    36661 ggatacaatg gtggaacaag gtggactagc aacaatcggg atcaatcaat atcaattagg
    36721 gcttcaaagc ggtaagatgg cggcagaact tgcgagtgga aaagaaaaac cggaaacgac
    36781 gcctgtttat acatttgatc aaggggatac agtgatcaat caatctcaag ctgatcgctt
    36841 aggaatcatt gttccccaat cgatgaagga aaaagccaaa atcattacag atgactcgca
    36901 gcaagaaaca tctaaggagg atgacaaatg atcgtatcag ctatttcaca aggcatgctt
    36961 tgggcaatat taggattagg gattttcatg acttatcgga tcttagactt tcctgatatg
    37021 acgacagaag ggtccttccc attaggtgct gctgtctgtg tgacagctat tactcatggg
    37081 gtagcgccga ttgtagctac attattaggc gtaggtgctg gcatgatagc aggactcgtt
    37141 acggggttac tttatacaaa aggaaagata ccggttattt tagcagggat tttagtgatg
    37201 tcaggattga actcagttat tttgtttgtg atgcgaacac caaatctttc tttattgaat
    37261 catccgattc tacaagacag cttccatcgc tttgggttac ctgattactt tgacacagtc
    37321 cttcttggct tcatctcttt gggcatcttg attgctgtac tgctattctt cttcaataca
    37381 gatttgggtc aaggctatat tgcgacagga gacaacgaaa cgatggctcg gtcgttaggg
    37441 ataaaaaccg atcgtatgaa aattttaggt ttaactttgt cgaacggtgt cattgcatta
    37501 tccggtgcgc tgattgccca gaacgacgga tatgcagatg taaataaagg gattggcgta
    37561 atcgtcatcg gtcttgcttc catcatcatc ggtgaagtga tcttccaaga attgacgcta
    37621 gcagaaagat tgattgctat tgttgtcggc agtatcattt atcagctatt gattttagta
    37681 gtaatcaaac tagggtttga cacgacttat ctgaagttgt tctccgctat catcttagct
    37741 atttgtctga tgatcccgca attgaagaaa gcactgaact taaaaacggg ttttgagaag
    37801 gaggcatagt aatgactgat atattgacaa tcaaaaatgg cagaaaagtc gtaaacaatg
    37861 gaatgaatga agagaaagta ctattggatg aaattaacct tacaatcaga gaaggggact
    37921 tcatcactgt ccttggcggg aatggtgctg gaaaaagcac actgttcaat acgatcgctg
    37981 gtacgcttca actcagtcaa ggatcgatct cttttaaaga tcagctgatc acaaaagatt
    38041 cagaagaaaa aagagcacga ttcctttctc gagttttcca agaccccaag atgggaacgg
    38101 ctcctcggat gacagtagca gaaaatttgc tacttgcaca aaaaagaggt caaaaacgga
    38161 aattccgttt acgtaacttg aaaaatcaaa aagaagattt tttcagacta tgtcaagaag
    38221 tcggtaatgg cctagagcaa cacttggata ctccagcggg tgaattatct ggtggtcaac
    38281 gccaagcatt aagcttgctg atggcgacga tcacagctcc tgagttacta ttattagatg
    38341 aacatactgc agctttagat cctaaaacag ctaaagcatt aatgcgatta acaaatcagc
    38401 ggatcaatga acaaaaattg acttgtctca tgatcacgca tcgaatggaa gacgccttga
    38461 aatacggaaa tcgtctgatc gtactccaaa aagggaaaat cgttcaagat atttctgaaa
    38521 aagaaaagca acatttgaca atgggcgacc ttttgcagtt ctttgaagaa gtagtggatt
    38581 gatttcgaca gaaaaattcc tttcagtaga aggacttgaa aactcattga catcttcctt
    38641 tgattcatcc tatacttaaa acaataaaca attaaacata tttgggtgtt ttgtaggatc
    38701 acgtagtcaa tgattttaac aattaaatag aatattcttt gctctgacaa gtaaacgatg
    38761 agaaaagaat ttgctacttt gccacctaga cgtttcgtac tttttcgtat caaacgctaa
    38821 gtggcttttt ttattgaagt aagaacaaag gaggaataag atgatttctg gacaaaccaa
    38881 attagctggg ctctttgctg atccggcttc tcacagttta tccccattga tgcacaatac
    38941 tgcttttgaa gcaaacagca tcgatgcggt gtatttagct ttccaagtaa atccaatgaa
    39001 tttgaagcaa gcagtcgaaa gtattcgaac ttttgatatg ttaggtgtaa atctttcgat
    39061 gccgaataaa acagcggtta ttccttatct ggatgaggta agccaggaag cgcaattgat
    39121 cggagcagtg aatacgatcg tgcacgaaaa gggtcgtttg attggctata atactgatgg
    39181 tgcgggcttc atgcaatccg tgcaagaagc tgggatttcg atcaaaaaga aaaaaatgac
    39241 tctactggga gctggaggtg cagcaaaggc agtcactgtt caagcagcgt tagacggcgc
    39301 tgaggaaatc gtagtttata aacgaaacaa ccaaacattc gctcaagtaa aagaaacatt
    39361 cgaacaactc agctatcgaa cttcttgtaa gataaaagtt cgtgactttg ccgatacgca
    39421 tcaattaaga aaagacttga aagaaagctg tcttctagtc aatgcaacag atatcgggat
    39481 gggaaagaaa atcaatcagt caccgctctt agatataagc atgatgcatg agaatctagc
    39541 ggtttttgat ttgatttatg cacctagaga aacacgttta ttaaaagaag caaaaaaagt
    39601 aggagcaaaa ggatacaatg gacttggcat gttgatctac caaggagcaa tcgcgtttga
    39661 attatggaca aaggaaaaga tgccgatcca tgtgattgaa aagctgtttg attaaaaaag
    39721 gataatagat aaagatcttt tattctcgca taaacggtct caagaaaagt aatcctttat
    39781 gtatcgaagc atttatttcg ttatttttta ttaaattatc aaaaaatgat aggcttttgt
    39841 cgattggtta tcgtatcctt tatttatcag taagaaacga ggaggaacca caatgagtcg
    39901 gatggatcgc tataaggaca tccataagaa ggcgaaacct ctgaagcggg atacggggag
    39961 tatttttgct agaagagaac gaaaacgaac gcgtatggaa aatgaggaag atgctacccg
    40021 tatttatgaa agagagcagc attcatttga acatttgaac caatacgagc aacagatata
    40081 cagagatgca ccaagagcag aagaacaaca aaaaaataaa aaatttttca atcggcaaaa
    40141 gaagaacaaa ccgttaaaac ctcgaagaaa gcgatcgtgg aaaaaaatca ttctatcgat
    40201 tttgcttgtt ttgatccttt attcaggtat ctcatttttt attggtaaat atgtagctga
    40261 acatgacaca agcttagagg cagcgacttc agaaaccttt catgggcaaa aagcgacgaa
    40321 tggtgcgcat aatattttga tccttggaag tgatacacgc ggagaagatg ccgggcgagc
    40381 cgatacgatc atggtcttac agttggatgg tccttcccat aagccgaaac tgatttcttt
    40441 tatgcgtgac agcttcgtta cgatcccagg ggtaggacaa aataagatca attcagctta
    40501 tgcatacgga ggagccgatc tagtgcgtca aactttagta gaaaattttg gaatcgattg
    40561 tcaatactat gcgaaagtcg atttcaaatc gtttgaaaaa gtgatcgatg ccttattcat
    40621 gaacggagtg aagatcgatg cagaaaaaga tctgaatctg gatggtgtag atattaaaaa
    40681 agggggccag aagatggatg gacatgttct tttgcagtac gcacgtttcc gaatggatga
    40741 acaaggagac tttggccgtg tcagacgcca acagcaagta atgaacgcta ttttcagcca
    40801 attgaagaat ccattgaatc tgatccgtgc accttatgca gctggtaaag ctttaggata
    40861 tacatccaca gatgtcactg cttggttctt gacgaaaaac ctcttttcga ttgcacgagg
    40921 agctggtggc attgatcgaa taagtgtgcc agtagacggt tcgtggaatt atgggcaaag
    40981 tgattatgct ggcagtattc ttgaaattga taatcaaatg aatcaatctg ctattacaga
    41041 ttttttgaat aaatgatcat aaaaaagatc aagaagaagc tgcgcaaggc ttttgtcatg
    41101 aatcaatcaa acgattcact cgtaaaaaac gctttcttat ggtagattta accaagagag
    41161 gagctgttta cattggatat ctatctgaac ttcaaaaatg agtcgcgtga agccatcgct
    41221 ttttatgaaa aagtttttca gactgaagca tcaggtatca ccacatacgg tgaaatgcct
    41281 ggaatagaac aaatgaactt gccagaagaa gcaaaaaatc taatcacgaa tgcacggctg
    41341 aacatttatg atacattagt tatgtttgca gatacgccag aaggaattgg tcctgaatta
    41401 gttgtgggaa ataatgtttc tttagtcatc cagacaagaa atgaagaaga aacgaagcgc
    41461 ctatttgaaa gcttagctga gggtggtaag attgagatgc cactggatgc tgttccgtgg
    41521 gctcagttgt atgggatggt aactgatcga tttggcatca actggcagat taaccaagag
    41581 tgacagctaa agatgaatat tttctggaaa catcaagcag caaacggatc atcgtttgtt
    41641 tgcttgatgt ttttgtgtct atttcgacta tacggtatct tttttttatt gccagatgcg
    41701 aaaatatgtt ccataataga gaagtccgca aaaaattaag taacggaggt cagaagatga
    41761 agacggtgat actagctgaa aaaccttcac aagcaaaagc ctatgccgaa accttccaac
    41821 agcaaaagcg gcatgaaggg tattatgaaa tcagcgatcc attgtttgct ggtgaagtaa
    41881 ccatcactta tggttttgga catttggtag atatggttgc tccaggagct tatgaagagc
    41941 gttgggcaaa atggtcttta gaaaatttgc ctattttccc agaaaccttc cgttacgaag
    42001 ttccaatgga caaacgtgca caattttcga tcgtaaaacg agaattgcag tcagccgata
    42061 ccatcattat tgcaacagat ggtgatcgag aaggtgaagc catcgcgtgg tcgattattc
    42121 gacaagcaaa cgcattttct aaggaaaaga actatttacg tttatggatc aattctcttg
    42181 aaaaagaagc aatctatgaa ggttttaaga atctccaacc tggaaagaac tattttccaa
    42241 aatataaaga agcgcaagca aggcagaacg ctgattggct gattggaatg aatggaagtc
    42301 cattatatag cttgctactt caacgaaaag ggattgatgg cagtttttct cttggtcgtg
    42361 tacaaacacc gacactctat atgatttatc agctgcaaga agaaatcaaa cacttcaaaa
    42421 aagaaacgta ctttgaagga gaaggtttaa ttacgacaaa caaagggcag ttttctggaa
    42481 aaatcgtacc aaaaaaagca tttaaaaccc aagaagagtt aacgaaggaa atagaaaaac
    42541 ttggagcgca tttaggtaag caaaacggac ggattcttga agttacgaaa aaggaaaaga
    42601 ggttgcatag tccacgttta ttttcgctat ctagtttgca gacaaagatg aatcagctaa
    42661 tgaaagcaag tgccaaagct acacttgatg ctgctcaagg attgtacgaa agcaagtttc
    42721 taagctatcc aagaacggat tccttttaca ttacagaaaa tgagcatcag tatctggtta
    42781 aacatctgca aaattataaa aaatttttag gaatagaaga agtacagaca actgagatca
    42841 aagcaaagaa acgatatgtc gatgcgaaaa aagttcaaga acatcatgca attattccga
    42901 caaagacgat tccgactcct gcacgctttg cgaagctttc caaattacag caagcaatct
    42961 atttgcaagt tttgcgcact actgtggcta tgtttgctgc agattatatc tacgaagaga
    43021 cgaccgtaat gacgggcgta cagcaattaa agctaaaaag catcggaaag atcccattac
    43081 agcaaggttg gcaggaaatc ttacgcatca agtcaaaaaa gaaagaagct cagactcttc
    43141 ctcctatcgt agaaaatgaa gaagcagaag tagaactgaa aagtatggag aaagaaacac
    43201 aacctccaaa accctatacc gaaggaacgt tgatcacagc aatgaaaact gcgggaaaaa
    43261 cgctggataa tgatgaagcg caagaaattc tcaaagaagt agaaggaata gggacagaag
    43321 ctacacgagc caatatcatt gaaaatctta agcagaagca ttacatcgaa gtcaataaaa
    43381 acgaaataac tgttacgcca aaaggaatca ctctttgtaa ggcagtagcc aatgaaccat
    43441 tactgacgag cgcagaaatg actgcgcgct gggaagggta cttgagaaaa attggtaaac
    43501 aagaggggac accatctgtt tttttagaga atatcaaaaa attcatccta catttattgg
    43561 cagaagttcc taatcaaatc gacaaggtag acttttcaga tgagattagc gggacacgtc
    43621 agataaaagc gatggagaag aaaaacgatc aattaggaag atgtccaaaa tgtaaaaagg
    43681 gaatagtcat gctttatcct aaaatcgcta catgcttgaa tccggaatgc gacttcaaat
    43741 tgtggccaac agtagctaaa aagaagctga ctaaaacgaa tctcagagat ttgctttcaa
    43801 aaggaaagac aagtaaagtt gtcaaaggtt ttactggtaa aaaagggaaa ttcgatgcag
    43861 ttttagtatt aaaagaagat atgacaatcg gtttttcttt tccatggatc gaaaacagtg
    43921 gaacaaaagc agttgatgga aacaaagaaa catcaagaaa aaataatgaa aacgaagtag
    43981 agtaaatgaa aacaatgaaa tagcggaata tttattccga aaagtgagaa tataaatacc
    44041 aataaaacta aaaaatatca gtgaatcaag caaaaacgtt ggggtaggct gattttatat
    44101 agatagcaag gagttttttt atttgaaaaa agtgcattca tttttaaatt atcttatggg
    44161 agcaatcacg attgtggctg tgtaccaagc atttctaaag tatcctgatt acgggacgaa
    44221 gttgttgtca ttgacgcaca atcaaacagc atattcacca acgttgattt tatcgacttt
    44281 gactgcagga gctaaaagcg ctacttattt ttttcataaa acgacaagca tgctaaagcg
    44341 aaagaaacag cgaaaacaat tgttggtggt ggtagcggct atgatcatta tttattttct
    44401 accagttatt acacaagtat ttcatcaata ttttggttaa gaatacgaga gtttgtgaca
    44461 aaagtcatgt cacaaacttt tttctttgtc attttttcgt aagaaaaagc aaaataaaag
    44521 tatatacatt gattaaaagt atatacttgg atataatgta cttactatag aatgagaatg
    44581 gtatagaggg tggcgaaata tgaaaaaaat gaaaaagtat aaaattagaa aatctggcaa
    44641 tagcgatgta acaactatcc caccagaagt aaaagaattc atgggtgtgc agacaggtga
    44701 cgcaatcagt tatgtttttc aatctgatgg ttctgtacga atgatcaaag cacaagaaga
    44761 acctgatatt gattcattag ttgattcgat catgaatcaa tatgaagacg cgttaaaaga
    44821 tttggttgat ttatgaggta tctttcgact aaagaaatta tcaaaatcaa cgcgaaagtt
    44881 attttacgtc actcgccagg cgaaatgatc ggtgtaaaag atgcaaatgc attagatatg
    44941 gccgtcaaac agccttcaca agcagtattc aatcaagaac tctatcctga aatctatgaa
    45001 aaagcggcga ttttagcaat taatttagct aaaaaacatc cttttcataa tggcaataaa
    45061 cgaactgctc ttgttgccat gttgttgttt cttcaattga ataattgtcc agttgctttt
    45121 tctcgtcaag aagctgttga ttttatcata atgattacca ctagttcatt ggatttcgat
    45181 tcactaaagt ccaagataac cagttattta cgacaaaccg caataaaatg aacgaattaa
    45241 aaactttatt tctcaattag atcagcatgt cattaaggaa tatgctgatt tttttatgtt
    45301 taatcataca aaatagtttt ttcataaaaa tatggaaaat atggattaat tttgaaataa
    45361 ttgatttaaa aggaatacaa aagagtgaat agtaggaaat a
//
LOCUS       DAEGIQ010000012        44941 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000012 DAEGIQ010000000
VERSION     DAEGIQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.012"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            212..604
                     /locus_tag="KMT45_000593"
     CDS             212..604
                     /locus_tag="KMT45_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBE7004080.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="KMT45_000594"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="KMT45_000594"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBE7004081.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="KMT45_000595"
     CDS             3165..4775
                     /locus_tag="KMT45_000595"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBE7004082.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="KMT45_000596"
     CDS             5067..5936
                     /locus_tag="KMT45_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBE7004083.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="KMT45_000597"
     CDS             6101..7372
                     /locus_tag="KMT45_000597"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBE7004084.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLITTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="KMT45_000598"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="KMT45_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBE7004085.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="KMT45_000599"
     CDS             8763..9026
                     /locus_tag="KMT45_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBE7004086.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="KMT45_000600"
     CDS             9471..9920
                     /locus_tag="KMT45_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE7004087.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="KMT45_000601"
     CDS             9977..11395
                     /locus_tag="KMT45_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBE7004088.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="KMT45_000602"
     CDS             11395..12255
                     /locus_tag="KMT45_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBE7004089.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="KMT45_000603"
     CDS             12429..13202
                     /locus_tag="KMT45_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE7004090.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="KMT45_000604"
     CDS             13349..13714
                     /locus_tag="KMT45_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBE7004091.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="KMT45_000605"
     CDS             complement(13862..15070)
                     /locus_tag="KMT45_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7004092.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="KMT45_000606"
     CDS             complement(15089..15367)
                     /locus_tag="KMT45_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004093.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="KMT45_000607"
     CDS             15521..16216
                     /locus_tag="KMT45_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBE7004094.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="KMT45_000608"
     CDS             complement(16658..17455)
                     /locus_tag="KMT45_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBE7004095.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="KMT45_000609"
     CDS             complement(17452..18636)
                     /locus_tag="KMT45_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBE7004096.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="KMT45_000610"
     CDS             complement(18661..19908)
                     /locus_tag="KMT45_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBE7004097.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="KMT45_000611"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="KMT45_000611"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBE7004098.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="KMT45_000612"
     CDS             21888..22202
                     /locus_tag="KMT45_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBE7004099.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="KMT45_000613"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="KMT45_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBE7004100.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="KMT45_000614"
     CDS             22887..23531
                     /locus_tag="KMT45_000614"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBE7004101.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="KMT45_000615"
     CDS             23546..23875
                     /locus_tag="KMT45_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBE7004102.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="KMT45_000616"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="KMT45_000616"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBE7004103.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="KMT45_000617"
     CDS             24863..25687
                     /locus_tag="KMT45_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBE7004104.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="KMT45_000618"
     CDS             25680..26027
                     /locus_tag="KMT45_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBE7004105.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="KMT45_000619"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="KMT45_000619"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBE7004106.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28180
                     /gene="dinB"
                     /locus_tag="KMT45_000620"
     CDS             27077..28180
                     /gene="dinB"
                     /locus_tag="KMT45_000620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBE7004107.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPL"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="KMT45_000621"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="KMT45_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBE7004108.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="KMT45_000622"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="KMT45_000622"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBE7004109.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="KMT45_000623"
     CDS             31657..32451
                     /locus_tag="KMT45_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004110.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="KMT45_000624"
     CDS             32441..34483
                     /locus_tag="KMT45_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004111.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="KMT45_000625"
     CDS             34764..34913
                     /locus_tag="KMT45_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBE7004112.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="KMT45_000626"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="KMT45_000626"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBE7004113.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="KMT45_000627"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="KMT45_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBE7004114.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="KMT45_000628"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="KMT45_000628"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBE7004115.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="KMT45_000629"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="KMT45_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBE7004116.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="KMT45_000630"
     CDS             39330..40412
                     /locus_tag="KMT45_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBE7004117.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="KMT45_000631"
     CDS             40412..41215
                     /locus_tag="KMT45_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBE7004118.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="KMT45_000632"
     CDS             41285..42025
                     /locus_tag="KMT45_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBE7004119.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="KMT45_000633"
     CDS             42036..43040
                     /locus_tag="KMT45_000633"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBE7004120.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="KMT45_000634"
     CDS             complement(43085..44929)
                     /locus_tag="KMT45_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004121.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14452 a         7837 c         9349 g        13303 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat tactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt tgagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgccggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 gattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatga gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 t
//
LOCUS       DAEGIQ010000013        38793 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000013 DAEGIQ010000000
VERSION     DAEGIQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.013"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(26..328)
                     /locus_tag="KMT45_000635"
     CDS             complement(26..328)
                     /locus_tag="KMT45_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7004122.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="KMT45_000636"
     CDS             complement(353..2446)
                     /locus_tag="KMT45_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBE7004123.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="KMT45_000637"
     CDS             complement(2935..3699)
                     /locus_tag="KMT45_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBE7004124.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="KMT45_000638"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="KMT45_000638"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBE7004125.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="KMT45_000639"
     CDS             complement(4704..5780)
                     /locus_tag="KMT45_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE7004126.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="KMT45_000640"
     CDS             complement(5804..6397)
                     /locus_tag="KMT45_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBE7004127.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="KMT45_000641"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="KMT45_000641"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBE7004128.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="KMT45_000642"
     CDS             complement(8067..8366)
                     /locus_tag="KMT45_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBE7004129.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="KMT45_000643"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="KMT45_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBE7004130.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11033)
                     /locus_tag="KMT45_000644"
                     /pseudo
     CDS             complement(10121..11033)
                     /locus_tag="KMT45_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            11153..12010
                     /locus_tag="KMT45_000645"
     CDS             11153..12010
                     /locus_tag="KMT45_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE7004131.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12197..12559)
                     /locus_tag="KMT45_000646"
     CDS             complement(12197..12559)
                     /locus_tag="KMT45_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBE7004132.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12905..14344
                     /locus_tag="KMT45_000647"
     CDS             12905..14344
                     /locus_tag="KMT45_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7004133.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14351..16903)
                     /locus_tag="KMT45_000648"
     CDS             complement(14351..16903)
                     /locus_tag="KMT45_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBE7004134.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17017..18348)
                     /locus_tag="KMT45_000649"
     CDS             complement(17017..18348)
                     /locus_tag="KMT45_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7004135.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18314..19792)
                     /locus_tag="KMT45_000650"
     CDS             complement(18314..19792)
                     /locus_tag="KMT45_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7004136.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20097..21932)
                     /gene="tdc"
                     /locus_tag="KMT45_000651"
     CDS             complement(20097..21932)
                     /gene="tdc"
                     /locus_tag="KMT45_000651"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBE7004137.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22402..23829
                     /locus_tag="KMT45_000652"
     CDS             22402..23829
                     /locus_tag="KMT45_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7004138.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23921..24286)
                     /locus_tag="KMT45_000653"
     CDS             complement(23921..24286)
                     /locus_tag="KMT45_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBE7004139.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(24283..24648)
                     /locus_tag="KMT45_000654"
     CDS             complement(24283..24648)
                     /locus_tag="KMT45_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBE7004140.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24840..25088)
                     /locus_tag="KMT45_000655"
     CDS             complement(24840..25088)
                     /locus_tag="KMT45_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004141.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25087..25215
                     /locus_tag="KMT45_000656"
     CDS             25087..25215
                     /locus_tag="KMT45_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBE7004142.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25272..26966
                     /locus_tag="KMT45_000657"
     CDS             25272..26966
                     /locus_tag="KMT45_000657"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBE7004143.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26970..27089
                     /locus_tag="KMT45_000658"
                     /pseudo
     CDS             26970..27089
                     /locus_tag="KMT45_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27617..28750
                     /locus_tag="KMT45_000659"
     CDS             27617..28750
                     /locus_tag="KMT45_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBE7004144.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28781..29101
                     /locus_tag="KMT45_000660"
     CDS             28781..29101
                     /locus_tag="KMT45_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBE7004145.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29130..29594)
                     /locus_tag="KMT45_000661"
     CDS             complement(29130..29594)
                     /locus_tag="KMT45_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7004146.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29726..30979)
                     /locus_tag="KMT45_000662"
     CDS             complement(29726..30979)
                     /locus_tag="KMT45_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7004147.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31008..31325)
                     /locus_tag="KMT45_000663"
     CDS             complement(31008..31325)
                     /locus_tag="KMT45_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBE7004148.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31351..31671)
                     /locus_tag="KMT45_000664"
     CDS             complement(31351..31671)
                     /locus_tag="KMT45_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7004149.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32154..33569)
                     /locus_tag="KMT45_000665"
     CDS             complement(32154..33569)
                     /locus_tag="KMT45_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBE7004150.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33586..34902)
                     /locus_tag="KMT45_000666"
     CDS             complement(33586..34902)
                     /locus_tag="KMT45_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7004151.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35052..35750)
                     /locus_tag="KMT45_000667"
     CDS             complement(35052..35750)
                     /locus_tag="KMT45_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBE7004152.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36039..36995)
                     /locus_tag="KMT45_000668"
     CDS             complement(36039..36995)
                     /locus_tag="KMT45_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBE7004153.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37108..37941)
                     /gene="msrA"
                     /locus_tag="KMT45_000669"
     CDS             complement(37108..37941)
                     /gene="msrA"
                     /locus_tag="KMT45_000669"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBE7004154.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38116..>38793)
                     /locus_tag="KMT45_000670"
     CDS             complement(38116..>38793)
                     /locus_tag="KMT45_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004155.1"
                     /translation="TSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLILTMIFFKNRR
                     YNKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMDYFL
                     YDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYL
                     GTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFF
                     KIVFHKR"
BASE COUNT        11682 a         8050 c         6593 g        12468 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc gggggggaaa ttcaatcgtc
    10261 agatccattc ctaattcttt tgccaagtcc atcatttggt tccactgcac agggaaagct
    10321 aggttttcca acaaatcttt acgaatctct tctgcgtcct gagtggctct tccatccgta
    10381 ttttttatat acaaacatct agcatcagat agatggacag actgcatata cggcttgaat
    10441 ttcttgaccg tatcattcat caagagacag tgagatggca cagggacttt cagcagcttc
    10501 gctgtttgcg cttggtgccg tttggcttct gccaacgtaa cggataaacc ttcgatactc
    10561 ccagataacg tatgttgcat cggacaattt tcatttgaca gataaactgg atggtcagga
    10621 tcaaatgttt ttgctacaat ttcctcagct tcgcttctcg tcacacctac tactacgccc
    10681 attgcataac cgcttggata agcttctttc attaagactg cgcggtgata gactagttcc
    10741 gctgcattct caaatctcaa agatcgacaa gcaacagctg cacaaaaagc accaatcgaa
    10801 tgtcctgctg caatatcagg ctgaataccg tttttttcca tttcatctaa ataaaatact
    10861 gcttttgtca gtaatgctag ctggataaaa accgtatctt gatagttttc ttctttatca
    10921 actaaccgat accccgtcac attttctaaa cgttccatgt acatttcagg gatatccgct
    10981 aacatcccta acttctgact gccttgtcca ggaaataaga aacctgtact catgagcctc
    11041 gcctcctcag cacttacttt ttccactgct atcttagtca atttctgcta taataaaaaa
    11101 tgccaaaaaa agaaaaaacc ataacaaaaa ggtatggatg ggatggataa ttatgaaatt
    11161 gcttcatctg cagtatttta aaaaagtatt cgaaacaaaa aatgttaccc aagcagccaa
    11221 agaattattt atttctcaac ctgccttgag ccgtgcgatc aaacatttgg aaaacgaatt
    11281 aggtgtccct ttgttttacc atagcggcag aaacattgag acgaccgttt atgcagaaga
    11341 attttatcca tatgcagtca agacattaga cacattagaa gaaggaatca aattattgtc
    11401 ttcggtcaat gagaagatcg ttacttccgt catattgtat ttagaggtag cctctgtttc
    11461 tattccgaac ttggtccgaa tttttatgga aaagcatcct gatatccaat taacgatcat
    11521 gcaacatcat ttaccagatg atattaccga tcctgtactg tacatcactt cggagatgaa
    11581 gcctggcatg accaatattc ctatcatgaa agaacctgtt tatgtcgcca ttcctaagaa
    11641 acatcctctt gctaaaaaag aacatctgca attagaagat atccaacgca cccccatctt
    11701 gatgctgtca aagagcaacg ccttccgtca tacactggat gcagcacttg aagcgaaaag
    11761 tatcgaattg acgatcagtt cgacgacaga tgatcctgcg acattacgtt ctattgtcaa
    11821 gcaaggatta gctgttagct ttttcccaaa agtttcttgg tcttacgata aaaatgatcc
    11881 tttcgtcttg cgggaaatca aagatttgcc cttgacacga acaatttatt tatcttctag
    11941 cttcacagaa gatcatcctt tgatcaaaat gattgcaaaa acgatgcggg aattttattt
    12001 aggaaaataa aaaaaaaaga gctgggacaa aattcttgtc gcagctcttt tttcgaacaa
    12061 acggtgttcc agaagtaatt tcttagaaaa taagccgaaa tttcccaaaa atttgagaag
    12121 caattttcgt aaattccttc ttatttcttg aagctaaaca tttctgtcac aacctcttct
    12181 ctattgtcct agacattcat tgcgttgatt taaaacttcc atcaatcaaa tctgctagtt
    12241 catctagctt ttctatttta cgtagatcaa tagagatcca atgttcttta ttcatgtgat
    12301 aggctggaaa atacccctct tttcctcgca aaattccgcc aagttcagga tcgaccttca
    12361 aatcaagaat attgatgatc ctatggtcac ttgcatcatc atagattttt tcttctggaa
    12421 cagacatcag gaccccaaac cattttttgc ttttttgatg tctgaatacg caataatcag
    12481 gaaatttttt aaaaatatat tctggagaca aattgtatgt ttcattgatg tgatcgatta
    12541 gttctttttt cgttagcatc tgcttccatc tctttcttat tgattcgctt tattcaaaga
    12601 taccacgtaa gaatagaaaa atgaatacaa gtagttgcta atcaaatact tcgatatttt
    12661 tcttttttat caactcttaa atgttcaatc cgcctgtgtt ggtcttgcta ccatctacta
    12721 ttaaaatgtt tattattgtt tgcccaccta ttttttgatt ttagtggtat catttttcaa
    12781 gttcatgatg aaattcttat catcagtaaa tgctccagaa aaataaacaa tgattgaaaa
    12841 atcagttgaa acttgaccac cgtttatttt ctccgtattc ttagaataga agggagtctg
    12901 attgatgtat tcactaatga aaaaaatcat taccgaaaaa gatattcgac gtcatgtttc
    12961 tttagtagaa caattgctga atcatacaaa gataaccgta aatgaattag ctgaaatcat
    13021 agggacaacc gaacgaacga ttttttctga ccttcaatct attcgctctc agctgcccga
    13081 aggctgggac atcatcagcg atcaagcagg aatcagtttg caaaaccagc aaaatttatt
    13141 aacaaatgac ctttgggaga tttttttcaa acaatcagtc agtgtcgaac tgctgaaaaa
    13201 tctgcttttt acaaaaaaag ttgctgtccc tgattttcta gccgaccatg ggctttctta
    13261 tgggacattg aaaagacatg tgactaaaat caatcagcgt ttagcaagtt acgacttgca
    13321 gatcgatttg acgaaatata cagcttgtat tcttggaaaa gagcgtgcga ttcgtacttt
    13381 ttatcatcga ttgctgattc ctttcactca taacaactat tttttcgagg attattcgat
    13441 ccatgaatcc cattattttc aatttttaag aaacctcagt caaactgaac tagcggttga
    13501 aacagaagag attttcggaa catgttggtt ctttatcaat acgatccgta taaaagcgaa
    13561 ttgccgatta gattcttcta tccataccaa cagcacactc tcctccctgt atgactctgc
    13621 attgaaaaaa ttgtatttaa cggaaggtat ttatcttaaa gacactgagc tttcttttgc
    13681 ttctttctgc tttctggaaa gttggaatta caacaataac tatggacaag aaaccgctcg
    13741 ctgcctgcat cattctccat ttttagaggt cctcgaaacg tttgtagagg aactagcttc
    13801 agagctgtct cttgatcaac taaaaaaaac atctctgact gataacttgg ctctcctcat
    13861 attaaaatat catgaatcgc cgatattgat tgaagcattg gaccgtcaat accatcgttt
    13921 tttagaaaca tatgatcagc aatatgcaca cttgtatcaa cagaaagaga atttactgaa
    13981 cgatctaaaa aaagaaatag aaatcaatga accaaattac tttctccaac tcttgtctct
    14041 actgatccaa caaacgattt tttctatcaa accgaatttg ttcaatgtct attttatttt
    14101 ccaaggagaa ccttcatgga aggcgttctt gcagcaggaa ttaaaagatt acttaggaaa
    14161 acgtgtctgc ttcttaccta tcgaactaac cgatctttcc actatttctt ttgaaaaaac
    14221 agatatcttg atttccaatt tccctctaga ttctctagat atccctattg tatacatttc
    14281 ttctattcca acgaaaaatg agctcaatcg tttgactgaa ctcactttca aaactttttt
    14341 atagtaacgg ctagcgactg tattctctag ctgttttttc ttttttgcga aacagtttgt
    14401 tcatgatccc tttttctatt tctacaacta agaatagaag aatcacattt gctccaatca
    14461 ataactgttg tgtcatcgtt aaacttactg taccgatcag ctgctggccg actggtagaa
    14521 aaataaccat cccttgcaat aaagcaagga tacctaatga aagaaaaagc gctttgtttt
    14581 gcaaaagacc ttggttcaat gacggcttgg acaactctct gcaattgatc atgtaaacag
    14641 cttgagctaa gacgatattt tgcagaagaa tggtttgctg taagctttgc ccttcaaact
    14701 gcatagccaa taaataggca ggaaccatga tcagtaggga gacatagatg atccgaaaca
    14761 aatgataacc agataggatt ccttgctttg gatctcttgg tgggcgcttc atgatttctg
    14821 gatctggcga ctcgaaccct agtgcataag ataaagtgat tgtcgttacc atattgaccc
    14881 ataaaatctg tactggggac aaaggtagcg ggcgattcag cataagtgcc caaatcaaga
    14941 tcaatccttg agctaatgca gttggcaaga agaaattgat ggttttttgc aaattatcca
    15001 aaatccgccg gccctctttt acggcttttg caatcgtatg gaaattatca tctcctaaaa
    15061 ccatatcagc tgcctgtttg cttacttcac tacctttgat tcccatagca attcctacat
    15121 cggatctttt caaagcaggt gcatcattta cgccgtctcc ggtcattccg acgatttctc
    15181 catttttctg caaagcttta acaattcgca gtttatgttc tggtgtcgtt cgagcaaaga
    15241 catcgactat tggcacttgc tgaatcaact cttcatcaga cataagatcc aaatcgatcc
    15301 cttctaaaac tttggcagta tgttttaagc caatttgttc cgcaatcgct tttgctgtgt
    15361 ctttgtgatc ccctgtaatc attttcactg agatccctgc ttgttggcat tcctttacag
    15421 ctttgactgc actttctttt ggcggatcga tcaatccggc gatccctgca aaagttaatt
    15481 gggttaaagt ctcatgcgtg agttcctgtt tcgaatctac ttttttataa gcaaatccta
    15541 aaacacgctg accttttctc gcaaattctg cagctgtctg actccatgct tcttgttctg
    15601 cagaagaaag agttgattta gcaaataata cttctggtgc acctttgacg taaatcaccg
    15661 ctcctttttc ctccgcatgc atcgttgcca tatatttgta ttcagaactg aacggaatct
    15721 ttgcttctat ttttttcaaa ggtagattgg ctgtttctgt gtgtcgaatc agcgcaactt
    15781 ctgtcggatt gccacttaaa ttttctgttt tctgttcttc ttttgttttt aattcctgac
    15841 agtttttcat aatggacaac acgcaggctt cttcttttgc agctacatcc acaactgtca
    15901 tttcattttt tgtcaatgtt cctgtcttat ctgaacagat cactgtcata gaacctaatg
    15961 tttctaccga aggaagtcct ttgatgatcg ccttttcttt tgccatttca tgtactccaa
    16021 cggatagaat catggtcaaa acagctggca atccttcggg taccattgac accaccaaag
    16081 caatcgtagc cgaaagaagg atatgccaat ccataccata tcgaaatgtc gtaaaaataa
    16141 ctaagaatag gctcaaacaa agaatgcctt gaaaaatttg tttgtttaat cggttgattt
    16201 ttttaatcag aggcgtcgtt tgttgttcga ccgattgcaa tgcttgattg attttaccga
    16261 tttctgtcgt gtttcctgtt tctacaacga caccgactgc agaaccagat tgtacaagcg
    16321 ttcctgaaaa acccatattt ttctggtcgc cgatcgattc ttcttctgtc aaaacggcat
    16381 gacttttttc agatgcttct gattctcccg tcaaaatcga ttcttccact aataaatcat
    16441 gtgcttcgat taatcggata tctgctggaa cgacatctcc ggcacgtaag acaatgacat
    16501 ctccttttac cagttctttc gcagggattt tttcttgcat cccgtttgat agtactatcg
    16561 cttcttgtcc catcagctgc ttcaaaccat tcaatgattc ttccgctttt ctttcttgcc
    16621 agtaactaac gagtccattg acgatcacta cgaataaaat aatacttcct tcaatatatt
    16681 cactagaagc aaatttcaaa aaagaggcaa tgatcaacac gatcatcagc agatcagtaa
    16741 aatgctttgc tatttttttc catcgttttg tctgctgcct tgcttttatt tcattatatc
    16801 cttgatcagt caggcgtttc tgtcgttctt cttgcgacaa gccttctgtt gatgtttgat
    16861 agtttttcaa tacatcttct accgtcaatc tgtaccagtt catcctatcc cctcctgttt
    16921 aattgatact gttattatac gcagggaagt tttttcaggc gtatcgatta tttcaggggg
    16981 gtctgaaata attcgttttg caaaatgtat ctgggcctaa ctgatatttc tattgcttat
    17041 tcttttatat acaaaattta agaatacaaa ccagataacc aataaaaaag gaataaagcg
    17101 tgtcgtgctt tcgaataaca agctgaaagc aattgaagta aaaaacacca acaaagcaag
    17161 atctttatat ctttgtgtag tgttccgttt cttttcttta ataaattgaa catgtgcaca
    17221 caagatcgaa gaccacacaa atagaaaaca actcgttgtc atgcctgcca aaagctgaaa
    17281 aaacaatcga ctatcagcta caaaaaaatg tagacaaaga ccggcaaaaa ataacattcc
    17341 tgtaattaaa ataccgtgta aaggtacgcc tttcttagaa agcgcacaaa accaatgggg
    17401 caaacttttt tcttttgctg tttgatataa aatcctgcta gtactgtaga ctgcactatt
    17461 tgctgaggac aaagaagcaa ccaaaatcat tagattgaca attgctgctc cgccttggca
    17521 atggatcact tgcagcatag tcactaaagg actttctttt aaatctaaac aatgccatgg
    17581 aacaacaaat aaaatcgaca aaatcgttcc tatatagaaa aagagaatac gccaaggcag
    17641 acagttgatt gcttttttca aattcttttg cggatcactt gtctcgccaa cggttaagcc
    17701 gatcatttca attccagcaa aactgaataa aaccatagga aatgagacta ccattccagc
    17761 aaagccaaac ggtagtaatt catcgatctg tccaaaattt tctttgattc cgatagactg
    17821 ggtatgaaaa tctgagacac tcaatcctat acccaataag ataaaaagga aaatccccaa
    17881 gatcttgatg attgagaaaa aagattccaa ttcaccaaac cacttgactg caacaaggtt
    17941 gactgccatt aagatcatta gtgcgctacc tccgcttatc aaagctggta catgtacaaa
    18001 ccagaaatgg atatacaatc ctattgctgt taattccgca agtccagctc caagccagca
    18061 aagccaataa gtccaattga caaaaaatgc ccactttttg cctaaataat agtcaacgaa
    18121 atcactgata gagttaaatg tactatttgc taaaaataaa tcacccatgg cccgcatcaa
    18181 accataaata gccaaaccgc atatcaaata aacgagaata acagctggcc ctgctgatgc
    18241 aattgccttt gctgaaccta aaaataatcc tgttccaatt gcgccaccta atgcgattaa
    18301 ctggatattt cgattagtta gagtccgttt cattgccggt ttttccatca agttcacctt
    18361 tttctttttc aagcagcatg gcgacttcct gttcccaaga aggtttcttt attttttcaa
    18421 tacagagtgg tactacaaac aaaatgacac tgatggctgt gactgcaatt agattgatcc
    18481 atgttccagc tttcatcgtc agtccgacag aaaatccgat tgccgcaaat aaaacaataa
    18541 cgacagtcca aactccaaga ttgctctttt tctctttttt tccaattcta aatggtcgat
    18601 tcaactttgc atgtgtataa cgcaatctca aaactgcgat ccccatgatg acatagacga
    18661 tacagtagat aacagtagtt gaatttacga gcatcaaaaa tgcttgattg acgccaggaa
    18721 tcagcaaata aaaaagtgca aagattgaga taatgacaga ctgagtcaaa ataacggatt
    18781 tggatacgtc aaattgattc gtcttccaaa aattgaattt tggtggatac gcaccttttc
    18841 tagcactttc tgtcactgtc tttgctggtc ctgaggccca agcagataat tggactgcta
    18901 caccaatgaa gacaagtaaa ctaaacagat tcacaataag atgtggcaaa ccaagaattt
    18961 ggcaataaat cgaaatagat tgcgcgatat tattcaattc catttgtcct tttggtacga
    19021 catttgctac aactaaagca ttcaaaatat tgacgaaaaa tataaaaatt aacgcggcaa
    19081 atactccgcg aatatacgtt ttaactggat tttccaatcg agtgatatac acagaggaca
    19141 tttcgatacc agtaaaaata aacatgatcg gcgcaaaata aacaaatgag gatgcagtcg
    19201 tagtatctgg tattaatttg tcccatgaaa aactgcctaa tgttccggtt ggtacgatcc
    19261 caactttgat ccacgcagca aaccctaata agagcatcat cgtcaaaggc acgtacaatc
    19321 ctaaccaaac accgactttt ccgccgattt ttgccatatc aaatttcaaa ttcaaaaaag
    19381 tgatgatcca ataaacgact aaaatgacaa ctaatgtaaa tttgctgttc aaaccaagcg
    19441 gcacattatc gataatatta ccaaacaacg gagcgagtgc tgaagcgacc atcaccatcc
    19501 caggaaacat ttggacccat aacagccatg agacaacgaa cccccatttt tttcctagag
    19561 cattcgatac ccaaagttct ggaccacctt tttggggaaa cattccagca aattcaccag
    19621 agatcaatga gattggaaaa gcgtacaata atactgcaac cagcatgtaa aaaatcatcg
    19681 tccagctcgt tgctgctaca tctggaatat ttcgtacact tgctacaagg gcgcaggtca
    19741 ttccgataaa ggtcatggtc gtgatttgct ttgatgaacc agtatctttc atctttattc
    19801 cttctttcaa tagcgacata tgtttttgtt ttcttttatt tttgttcaag aacagaaatg
    19861 acaaggtttc atcgattcag tacaaaaccc agtatcaccg aaacatccat tgaacgatct
    19921 tagtgaactg ggtttgatac atgaaacgaa cgatccaact tatagaacgt tttataaaaa
    19981 agaatatctt ggcgaggaat atatgaaaaa cagggggaaa tacaattgtt ttgatgaagc
    20041 aaataattgt ctgttctcca tatgtctctt gttattcttt cacacaaaaa tcagttctat
    20101 tggatgattt cgtttagttt cttttccatt gctttggcga ttttttttgt ataaaaattg
    20161 aaaattcgat catcattcaa ataaggagtc atgatagccg cacgtagcag agtgattttc
    20221 tgttcttttt tccattcttc tttcgttaga cccattcttt caacaaaacg aatcggagaa
    20281 tcaccgtaat cttcttgtgt aaatgtagta tgagaagtaa taaaccgttc accataaaca
    20341 tcgccatcca tataagaaga ccgatcgaac attttttcat ttaattcatt gatagcgttc
    20401 aaatctgtac agccttgttc tttgaatacc caattgacca tattaaaatc aggatgattc
    20461 aatggataga cttcgattgt ttttccttta acagtaaaag tcagatgatc gagaaattct
    20521 ctgaatcttt gagctgcctc gattgaagcc ccaatcaatt gaccatatcc ggtcacattc
    20581 aatggcaata cacgatgtgc tgtccagact gcggcggcgg ttgctccagc ttttgatcct
    20641 tccaaaatat aagctcctaa catatctgga gcctttactg atttttcaaa cacatatggc
    20701 gcgaaataag aaatgatatt tctcatattt ttatgtttga ttacgattcc tccagcagca
    20761 taagggacat agcccatctt atgaggatct atcgtgacag aatccgcttc tgaaatagct
    20821 ctgaaccctt catatacctc tttgtcaatc gttacgcagt ggtggaaaac atgatgttct
    20881 tcaaagaatt cagctaagga agcatacggc acaaattctc ctgcttcgtt tagaaacagt
    20941 gaacgcgcat agccgccata tgcggcatca acatgtaaat agaaatagat cccttcatct
    21001 tttaaccttt ctcttaattg gacgatttta tctacactgt ctacttgacc ttcttctgtc
    21061 gttcctacga cagcaaccac acctagaatt gggatttttt gtccagctaa ttcacgaatt
    21121 gttttttcca aagcattaat atccattcga taatcctctt gaactgggat agcaaccatc
    21181 tgatctaagc cgactccgca aatatctaac gccttcatcc aagaatagtg ttttgtttga
    21241 gggaccagcc atttccctaa tctttggata ttttttccgc tacgggaaga agctgctttg
    21301 acctcatcca tttcttcgtc cgtaaagctt tcggtcattt ccaaaatttc ttcaacagat
    21361 aaattcaaaa gttcccactc agacattttt ttcacttttt caggcagcac ttccttgaca
    21421 gctaaaggaa tcgatttgat gcaacgggcg taccataatc cttcaagatt agcaatagaa
    21481 ccatctgctg caatgtgtcc ccatccgtct gtcatattga ataatgaagc aaaatcctgt
    21541 cctacttctg cttccatttg cgaagttgcc atagaagatt ccaatgcaac attatttggg
    21601 ttccacagca tcgcatagtt gtaagctaat aaagcaggca tcaatgtttc agcattcatt
    21661 tgtccccagt aacggcctgc tgaatgccat gggatcgatc ctgcgcgtaa tcgctgactg
    21721 acttcatcca agacctctat catatggtcg cgggtggcta gataatctgg agtgagctta
    21781 tctccttcgc taattgcagg catatctgaa ggaatataat tttctctcca tccaagatgt
    21841 tcatctacca tcttgttcaa caacatttta taaactggtc cattttcagc tttgtcgcct
    21901 ataaagacgg ccttgatatc catatctttc atttttcttc cctccatatt ttgttggtca
    21961 atgtcttgca gatacatgtg tttatctgta acacctcttc actatagcgc caaaaatgag
    22021 aaattttttt ttataacatc aaatccacgt agatttttac aaacaaaaat aaataaagtc
    22081 ggatttttct gataattcaa cgcaaaaaag cgaacaattt ttcaataaat cagtcttcta
    22141 aaatagataa cctaaaaaaa gcgaacaaac aaataaaatg actgtccttc attcactaaa
    22201 catattttta tttttttata ttgttaaaaa ttcacaattt attttttaat agaattatct
    22261 gttttccaaa ttttataaca taaaaaacct tttttaacgg catttatatg ctttttcaga
    22321 aattaactga tgatttcaaa aagaaaacat gtcataatat ttcccatatt gtgcaaaaaa
    22381 atacaaggcg gaggctctac tatgattgaa gaatatattg agaaaaatat tttaagacag
    22441 ctgtttttat gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat
    22501 ctcctgcatg tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt
    22561 gaagaacaga tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa
    22621 catatacctc gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact
    22681 tgtctgcttt atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc
    22741 atctctgtca gcaaagctta ttcattaaaa aaacaagtat tagcttactt taatgactgc
    22801 gggatcgaaa tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt
    22861 cttaatgtgt cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt
    22921 gaacgtgccg atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa
    22981 gaaaataaag aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt
    23041 gctgtgacaa tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat
    23101 gtcgaatcag catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc
    23161 tttattttga cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg
    23221 gaagactttc aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta
    23281 gttgctacct ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt
    23341 gcgctgattc atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa
    23401 ttttatcttt taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc
    23461 agctggtcca gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt
    23521 gtgatcgaat tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc
    23581 acaaattcgg atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc
    23641 gattttcaag ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag
    23701 cagtcctcaa accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat
    23761 gaaaatatta ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa
    23821 aacaaataag gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca
    23881 tttctgtaac aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta
    23941 ggggtccgat ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg
    24001 ctagttggaa aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa
    24061 caagaatttc caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct
    24121 cgcttactcg gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga
    24181 ttgatagaca aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa
    24241 gtttttcgaa atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct
    24301 tctttgtttt tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat
    24361 gataataata agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat
    24421 gatatcagca acgactagta cttccaagct taacaaaata taacttgcta gcagttttct
    24481 tgtatctgca ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa
    24541 aagtttgaat agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa
    24601 aatagcaatg atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta
    24661 ctttctttta atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt
    24721 taaaagttgt tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt
    24781 ctccctccat ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct
    24841 tagtccgtca tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt
    24901 ctaaaaatag aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt
    24961 acaggaaaat cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc
    25021 atttggcatt ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg
    25081 tttttcatga gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc
    25141 gctgcatatc taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa
    25201 ttcgaatcaa tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg
    25261 gaggaatcac aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg
    25321 cagcggctgt ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt
    25381 cattggatga tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt
    25441 ggaataaaaa ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc
    25501 ttaattaccg tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg
    25561 agccagggat gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg
    25621 acaaagcacg tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca
    25681 ataataaaga ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa
    25741 aactagacaa tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa
    25801 acttctggtt tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat
    25861 tgcgtcgcta tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg
    25921 tcaatcgaac acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga
    25981 aagaacaagg atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa
    26041 caccgatgca agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag
    26101 aagaagaata cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta
    26161 cagattcagc tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg
    26221 cagcttctag tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca
    26281 aattctttgc tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc
    26341 atttgttgtt gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt
    26401 catttatttc aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctattg
    26461 tcgtgcatca tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt
    26521 atttccttta tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg
    26581 gaaaagaaat gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga
    26641 atattcgtga aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata
    26701 tgccttatgc ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc
    26761 ctaaacattc gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga
    26821 tctttactga acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta
    26881 ttcctatgtc ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc
    26941 gtgccgcaaa aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag
    27001 taataagaac aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac
    27061 cgcagatgca agcttttgaa caccgtttac caagaggtta tgaaaaagct gccctgcatc
    27121 aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat
    27181 taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt
    27241 ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact
    27301 taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt
    27361 tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata
    27421 caactatttc aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa
    27481 atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta
    27541 agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct
    27601 gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag
    27661 aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac
    27721 agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg
    27781 gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac
    27841 attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt
    27901 ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg
    27961 gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag
    28021 agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa
    28081 agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt
    28141 aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa
    28201 attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct
    28261 agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc
    28321 tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg
    28381 aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg
    28441 cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt
    28501 aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga
    28561 tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat
    28621 gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga
    28681 agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt
    28741 gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa
    28801 tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg
    28861 cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat
    28921 tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta
    28981 tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa
    29041 catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg
    29101 agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga
    29161 gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa
    29221 aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt
    29281 ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac
    29341 agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc
    29401 gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc
    29461 ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt
    29521 gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag
    29581 ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa
    29641 gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat
    29701 cacttgttct ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga
    29761 tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg
    29821 ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta
    29881 cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa
    29941 cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga
    30001 taggtaaccc aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt
    30061 ttgttacaac catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac
    30121 ctaaagttgc acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga
    30181 taaacggtgc gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat
    30241 tgattgtttg catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga
    30301 atggtgtgat aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg
    30361 gttcttggac cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa
    30421 ttgcagcaac taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg
    30481 cttttgcaac tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc
    30541 ttaaacgtac aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt
    30601 ttgagctcat acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg
    30661 cggaaacacc gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca
    30721 agacagaaag aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt
    30781 caccaccaaa aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca
    30841 ttactgctaa tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg
    30901 caactaaatg ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa
    30961 atgcaacaaa tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat
    31021 cgacgataca gatcaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga
    31081 tgatcttgac tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt
    31141 gtcagcatct ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt
    31201 ttttcatctg ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg
    31261 ctttttgcat ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct
    31321 tccatttatt gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc
    31381 gagtactttt tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat
    31441 gcctttaggt gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa
    31501 aagtaaaacg tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc
    31561 aaagatgtct gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag
    31621 acttgtactc attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac
    31681 gttcctttct atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt
    31741 taaaaagttg atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga
    31801 aagcgcacac taaaaaagaa agcgcacact aaacacacaa attaattttt attacctttt
    31861 ttcttttaac gtgcacaaaa aaagttgtga cacaaaactt gtcacaactt ttttcaaata
    31921 aacggtattc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataagat
    31981 gttgttttta ctttgctatt cttattactt gatgcaggac agctttttca caacctctca
    32041 ataaacggtg ttcaatcagc tgaccctggg tattagctca aaacgctgga ttcacaacct
    32101 cttttatttc aaataaccag taaatacaat gaacatctac aaaaaacttc tatttattta
    32161 tagatttcgt caggcaaatc caattctctc ttatcaatca aagtctgata ccacgaagca
    32221 gattttttca atgttctgct tcgttcatcg ttcaattcga ttcgaactaa tccatatcga
    32281 ttcttaaatg cattcattgg tgacacacag tcagtgaatg cccataacat atagccttcg
    32341 caatttgcgc cttcttctgt tgctttgatc aagtaatgca aatgctgtcc gataaattcg
    32401 atacggtaat catcttgcac gattcctttc tcatccatat actgttcttc atattctctt
    32461 cccataccgt tttctgttat cagccaaggg atattatgat aattttcttt tagatacatg
    32521 gctatatcat acatgatttt cggatagatt tcccatcccc tggaattatt cattttcttc
    32581 ccaggtaaat caaagacttc ataatatcgt tcaggatgga aaggtgtctc ttcattccat
    32641 tgataacgag gggcgcgaac gcgtttagga taatattgat tgattccaag gaaatctact
    32701 gtatgatgct gaatcgtttc taaatcctct gttgcaggat cgaaataaat cgattgtttt
    32761 tgacacaact ccaggaactc ttctgtgtag tatcctttta ccatcggatc aaaaaatact
    32821 cgattataaa acaaatcata catataagca gctttttggt cttctggaga attggatcga
    32881 gcatagacca tttcaggatt taaaatacag ccaatccttc caccataatt tccgctgcgg
    32941 taaatctcta ctgctttagc cgttgctaaa actttatgat aattccacat catccacttt
    33001 tttgtgtttt gttcatgcgg ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg
    33061 attggttcgt tgaatgtaaa ccagtttttt accctatcac cgtaacgatc aaaggcaatt
    33121 tgagcatact tagcataaaa atcaactact tttcttgaag accagccatc atatttttcc
    33181 aaaagaaaag ctgggacttc ataatgttcc aaacaaatca tcggttctac gtctgcttcg
    33241 atcaaagagt cgatcatttc acttatatgc tgagcatatt cttcatctac tatacagttc
    33301 tcataatcag taaaaaaacg agcccaattg atagaagtcc gataatgtgt cagatggatg
    33361 gatttcatca gatccacatc ctgctggtat tgttccataa aattagttgc tacggctggg
    33421 ccatacccat tatgccaaac aaaatgttca tttttatacc agctatctat aaacgaatcc
    33481 tgtccttctt tttttccttt ccaaccttcc gtttgccaag cagaagatgc agcaccaaga
    33541 ataaaatttt ttggaatagt aattttcatg atttcctcct gttttttaga tcccatcatt
    33601 ttccacacga tttgctactt ttacaaatgg cagatagatc aaaatagaaa caataataca
    33661 aacaagttgc gtaataactg caccgatatt tcctgctgtt gccagataag cattgacgat
    33721 cggcggcatt ggccaaggga ccattacaac tgctttcgcc gcaaatccta aagcggttgc
    33781 aaaataacca atcgttccag tgataagagg cgttaagata aacggaatag ctaaaattgg
    33841 attcagcatg atcggcatac caaaaataac tggttcattg atgttgaaga tcccaggagc
    33901 aagggataac ttggtgattt gtttcatatc ttcccgtttt ccaactaaca atactgcaat
    33961 cagtaaaccg atcgtgactc cagacccgcc catactcata tacatatccc aaaatggcat
    34021 agtaataatg ttcggtactt cttttccagc ttcaaaagct tccgtattta cagcaattgc
    34081 cgctaaaagc aacggttccc gtatcggctt caccatttga tttccatgga taccaatcac
    34141 ccaaaagact tgcgcaataa acatcaatag caaaataccc ggtaaccctt gtacgacacc
    34201 ttctaatggg cgttgtacga cattgtaaat gatatcataa aggtacattc ctgtaagttc
    34261 tttgatgata aatcccgcag ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc
    34321 agaaaaacta gcagaaacat tcgttggcac ggtatcaggc attctcagct gtagtttctt
    34381 ctgtttcctc aaccagcaat aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa
    34441 taaccctttc gtatctgtat attgtttagc caatacgttt tctactataa cagtctgatt
    34501 ttcattcact aattcataaa cagttggatt aacagataaa taagacatca ctgcgatgat
    34561 accagggaat acaccatcca ctttattcag cttggctaat tcgatcccta tcagaaaaac
    34621 agcatagatg gtcaagaaac ttagtgtgac atagctgatg gcagatgcga taggctttaa
    34681 ttcttctaaa aaacgcaaga aacttatctg tgctaagcca ttttccgagt caaagaccat
    34741 tgctgaaaac aatgtgccaa aagcacccgt aataattacc ggaatcaatg cagcaaatgc
    34801 gttttttatc accatgatat accttaaatt attgattctc gttgccacgt tccctaaaac
    34861 attggctaat ttatccataa aactcattcc agatcccctc attcctgctt cttctccttg
    34921 acgtcatctc tttttgtcta ttagtcagcg cttacatttc gatgaacaga aactatttac
    34981 tcctcttttt taccaaatat aaaacaaaaa ttcatctgat gtatttatca taaaatacat
    35041 cagatgaact gtcaattagt ttctttttct cctattattt tctgtaattc tctccgattt
    35101 attgccatat ggacagtcat tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc
    35161 tgaacgatct ctgtatgaat atcgatcgtc tcgattttca gatgctggat atcataattt
    35221 ttattaaaca aagcaattga ttcattaatg attgggataa tatgcagcaa ggctacgttt
    35281 ccgctagctt ctgcaatcat cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg
    35341 ccttctcgaa aagaagtttc aatcttttgc gttaattttt tcatttggac gatttgattc
    35401 ttcgttgcat attccgctgc caaacctgcc atcttaggct ccaataaata tctcagttca
    35461 aataagtctc gtatcatccg atcctgatct tcaatcaaag aaaaaccgag tgggtcattg
    35521 gcaatccctc gcttatcact gacaaatgtc cccgctcctt gccgaacttc aaggatattc
    35581 ctggaagcta gagcccgcac agcttctcgc accgtgcttc gccccacctc taaataatcg
    35641 gacaattcat attcattcgg cagtttatct cctacttgca actgcatttc ctttaaatat
    35701 ctaaggatct tgtcagtagt tacttctact agggttttat tttcttccaa accacatacc
    35761 tccttttcat ctgatgtatt ttttcgtaat tattataaca cgtttgaatc atctgatgat
    35821 tttttgtata caaaattgat taatccgttc atagacatac aaacgaagaa gatatttacc
    35881 ataaaaaaga ctaggataaa attcactcct agccttcgat catttttatt ttgaacgaca
    35941 aaaaatatga tgttcaataa cagacgatct tcattcgatt cttgatgtca tcttttgttt
    36001 tttttaatat atggataaaa agtttgatta catcgtttct attctagaat ttggtcgatt
    36061 ctcaattctt cttctggtga gaaatctaaa ttatccatca ttcgaacact ttcttgtaac
    36121 tgacttaacc gacttgctcc gaccagtaca cttgtaacag atttttgtcg caaattccat
    36181 gcaagggcca tttgcgctaa tgattgtcct cgtgatacgg cgattgtttg cagtgctttg
    36241 attttttcca gagttgatcc aacttgttct tctgaaagga atggtatttc cttacgatgt
    36301 gctctagaat cttcaggaat accatgtaga taacgatttg tcagcaaccc ttgagcaagc
    36361 ggactgaacg tgattgcccc tagcccttct tcttctaata ctggagatag atcgtcttct
    36421 agtagtgcac gagaaaacat attgtagcgc atctgatgaa taatgaacgg tgcttccttt
    36481 ctttttaaaa tttcagtcat tttttttgta tcttctccat tatagttgga tataccaata
    36541 tataatgctt ttccttgtct tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt
    36601 gtatcagggt ctgggcgatg atgatagaag atgtcaacat aatctagccc cattctttgg
    36661 agactttggt cacaacttga aatgatggat ttacgagagc cccattcccc atatggccct
    36721 tcccacatat gataacctgc tttgctagaa ataatcagtt catcacggta ggcttgaaaa
    36781 tcttctctaa aaatacgccc aaagtttttc tctgcagctc cagcagggcc tccataatta
    36841 ttcgcaaggt cgaaatgggt aattcccatg tcgaatgcgc ctcttaaaat ctctctttga
    36901 ttatggatag gatcataatc accaaaattt tgccataatc cgagagataa aatcggtaat
    36961 ttcaaacctg aatgaccgga tttacgatat tccatttttt cataacgtgc actatctgca
    37021 atatacaatt tttctcctcc tcatcaaatc tttttaaaac gctttctttt ttctattttt
    37081 agtttactac attttaaaga aaaggagtta atcaaaatga ttttttcgta tatttttttg
    37141 ccatttgttt tttactcgtt gatagatcag aaattgatta cgtgcttgct ggattttttt
    37201 ataacgtttc ggttgttttt tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt
    37261 accggctggt tcgatttttg tgacgattgg ttggtggaaa gttcctgaac tgtctagttt
    37321 ctgttttgat tgttccgcta attctttttg tcgttcgact tgataaaaaa tcactggacg
    37381 atattgcttc cctctatctt gaaattgtcc aaatgtatct gtgggatcag taatttgcca
    37441 atagattgtc agcaactcgc tataacttat ttctcttgtg tcataaatga tctccacagc
    37501 ttccacatga cctgtgtaac caccacttac ttgatcataa tttggttttt caacatgtcc
    37561 gcccgtgtat cctgacaaaa cagataggat cccttttttc gattcaaacg gctcgaccat
    37621 acaccagaaa catccccctg caaatactgc acgttcttga tagtcagcat cttgcatggc
    37681 atgcttggaa aagtcatatt tttgctcctt atcaaatcct tccgtaatca ataaataaaa
    37741 gtccattaca tcaggcgtca gattcattcg gatagctaat ggacgtagtg ttgcttctag
    37801 ttttgataac tgttctttaa cggatatatt ttctcttatg tcattctttg tctgaatcaa
    37861 tacttttcgt tcccaatctc gtgtattcgg attcaaaatc agattataaa gtgtttgaat
    37921 gaatatttgt tcctcatcca tagattttcc tcctttccga agaagacaag ctagtgactt
    37981 tagctgtaat ttatcataaa gtgaggaaat aagcgattac ttcatccccc tacagatttt
    38041 tcgtataaaa aagagagaat ccttatcgac agattctcct ttttaatcaa tttattttat
    38101 aaatgaaaag ttaatttacc ttttatggaa aactatttta aaaaataaac ataaatatgc
    38161 agttaatgta acaagataag aaataatagt cgttactgta taacttgtgc ttactttaat
    38221 atcagcaact cctttgactc cgcttatttg taccaatcca ttagcattca cctgctcctt
    38281 tacaggttga tgatttattt ctacattcgt tcctaaataa gcatatacag gaagtgtgat
    38341 tacttggctc ttctcattag ttactctata tatgatagaa ctaccagttg ttttataagg
    38401 aaggtttacc cattgactgc caatctttgc ttcgtgtttt attatatgct ccttattctc
    38461 caatgaagcg ataggagaat aatcatataa aaaataatcc attcctttag tggtatacgc
    38521 ctctactgtt tctttgttta caacaatatg tcccggctga gaaattattt tttgattgaa
    38581 atttttttta ctagaaacag ttaaagtgat taataacaaa acagtcataa ataaataaaa
    38641 tagttctctt gtcttattgt atcttctatt tttgaaaaat atcatcgtta atatcagaga
    38701 agagaatatt atttgcaagc ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt
    38761 attttgcaga agataccatg gcaccaaact agt
//
LOCUS       DAEGIQ010000014        38433 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000014 DAEGIQ010000000
VERSION     DAEGIQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.014"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="KMT45_000671"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="KMT45_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBE7004156.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="KMT45_000672"
     CDS             complement(2204..4390)
                     /locus_tag="KMT45_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBE7004157.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="KMT45_000673"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="KMT45_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBE7004158.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="KMT45_000674"
     CDS             complement(4612..5052)
                     /locus_tag="KMT45_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBE7004159.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="KMT45_000675"
     CDS             complement(5126..5665)
                     /locus_tag="KMT45_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HBE7004160.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6280..7746)
                     /locus_tag="KMT45_000676"
     CDS             complement(6280..7746)
                     /locus_tag="KMT45_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7004161.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8057..10138)
                     /gene="cadA"
                     /locus_tag="KMT45_000677"
     CDS             complement(8057..10138)
                     /gene="cadA"
                     /locus_tag="KMT45_000677"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBE7004162.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10662..11021)
                     /locus_tag="KMT45_000678"
     CDS             complement(10662..11021)
                     /locus_tag="KMT45_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBE7004163.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11051..11392)
                     /locus_tag="KMT45_000679"
     CDS             complement(11051..11392)
                     /locus_tag="KMT45_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004164.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11389..12063)
                     /locus_tag="KMT45_000680"
     CDS             complement(11389..12063)
                     /locus_tag="KMT45_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBE7004165.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13240..14538)
                     /gene="asnS"
                     /locus_tag="KMT45_000681"
     CDS             complement(13240..14538)
                     /gene="asnS"
                     /locus_tag="KMT45_000681"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBE7004166.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14578..15768)
                     /locus_tag="KMT45_000682"
     CDS             complement(14578..15768)
                     /locus_tag="KMT45_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBE7004167.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15789..16316)
                     /locus_tag="KMT45_000683"
     CDS             complement(15789..16316)
                     /locus_tag="KMT45_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBE7004168.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16363..19152)
                     /locus_tag="KMT45_000684"
     CDS             complement(16363..19152)
                     /locus_tag="KMT45_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBE7004169.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19299..19499)
                     /locus_tag="KMT45_000685"
     CDS             complement(19299..19499)
                     /locus_tag="KMT45_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBE7004170.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19951..20271)
                     /locus_tag="KMT45_000686"
     CDS             complement(19951..20271)
                     /locus_tag="KMT45_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004171.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(20652..21770)
                     /locus_tag="KMT45_000687"
     CDS             complement(20652..21770)
                     /locus_tag="KMT45_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004172.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            22241..23548
                     /locus_tag="KMT45_000688"
     CDS             22241..23548
                     /locus_tag="KMT45_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBE7004173.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAENTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            23667..24866
                     /locus_tag="KMT45_000689"
     CDS             23667..24866
                     /locus_tag="KMT45_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBE7004174.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIFYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(24893..26161)
                     /locus_tag="KMT45_000690"
     CDS             complement(24893..26161)
                     /locus_tag="KMT45_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBE7004175.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(26324..26971)
                     /locus_tag="KMT45_000691"
     CDS             complement(26324..26971)
                     /locus_tag="KMT45_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBE7004176.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27379..27882)
                     /locus_tag="KMT45_000692"
     CDS             complement(27379..27882)
                     /locus_tag="KMT45_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBE7004177.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28005..28769)
                     /locus_tag="KMT45_000693"
     CDS             complement(28005..28769)
                     /locus_tag="KMT45_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBE7004178.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSK
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNIYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28876..29151
                     /locus_tag="KMT45_000694"
     CDS             28876..29151
                     /locus_tag="KMT45_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBE7004179.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29828..30778
                     /gene="yidC"
                     /locus_tag="KMT45_000695"
     CDS             29828..30778
                     /gene="yidC"
                     /locus_tag="KMT45_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBE7004180.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(30957..31931)
                     /locus_tag="KMT45_000696"
                     /pseudo
     CDS             complement(30957..31931)
                     /locus_tag="KMT45_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
     gene            complement(31928..32386)
                     /locus_tag="KMT45_000697"
     CDS             complement(31928..32386)
                     /locus_tag="KMT45_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE7004181.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKDVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTKIATILTKEIGEDI"
     gene            complement(32383..33846)
                     /locus_tag="KMT45_000698"
     CDS             complement(32383..33846)
                     /locus_tag="KMT45_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBE7004182.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAVDTLEPEVMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEKGRAGDLS"
     gene            complement(33828..34778)
                     /gene="pfkB"
                     /locus_tag="KMT45_000699"
     CDS             complement(33828..34778)
                     /gene="pfkB"
                     /locus_tag="KMT45_000699"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBE7004183.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHSFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTDFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRVLQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAEND
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIGEERFERWENIN"
     gene            34921..35670
                     /locus_tag="KMT45_000700"
     CDS             34921..35670
                     /locus_tag="KMT45_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBE7004184.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVIAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARLLLDAYVLRMGEN"
     gene            complement(35944..37077)
                     /gene="wecB"
                     /locus_tag="KMT45_000701"
     CDS             complement(35944..37077)
                     /gene="wecB"
                     /locus_tag="KMT45_000701"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBE7004185.1"
                     /translation="MKIKIMTIFGTRPEAIKMAPLIKAIENDERFESIVTVTAQHRQM
                     LDQVMDIFDLKADYDLNIMKDGQTLTDITSRVIKELDSVLVEAKPDIILVHGDTTTTF
                     AASIAGFYHQIKIGHVEAGLRTWNKYSPFPEEMNRQLTDTLADIYFAPTVMSKSNLLK
                     EGRPEESIFITGNTVIDAMEYTIKQNYSNELLDNLAGKRIILVTMHRRENLGQPMTNV
                     FKAINRLVEKFEDVHIVFPMHKNPKVRKNAEETLSDSEQVHLIEPLDVIDFQNFSNNS
                     YMILSDSGGVQEEAPSLGVPVLVLRDTTERPEGIEAGTLKLVGTEEDKVFEEATLLLS
                     DKEEYEKMSQASNPYGDGNASERILDAIAYNFGIQQEKPIDFQ"
     gene            complement(37289..38433)
                     /locus_tag="KMT45_000702"
                     /pseudo
     CDS             complement(37289..38433)
                     /locus_tag="KMT45_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT        11012 a         8171 c         6683 g        12567 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagggg ctatttttgt ttggctgttc ttattacttg atgcaggaca
     6121 gctttttcac aacctctgat tcacggtgtt ccagaagtag cttctgcgaa aaacaagccg
     6181 aaacttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     6241 ccacttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa ccactgtttc
     6301 tggttcagtg atttctttgt ttttgtctcg acgagcaatt gctggcaaga tcatacctaa
     6361 taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc atgctttagg
     6421 atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg cagtgaaggc
     6481 aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt tatattcaga
     6541 attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc ataggtaacg
     6601 cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat tcatatcttt
     6661 gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca gaagataacc
     6721 attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa attctggatc
     6781 cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg cggagatttg
     6841 tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac catagtaatt
     6901 acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag gaatattatc
     6961 gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc ctaccattgc
     7021 tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag agttgacata
     7081 aggggatatt ttttccgctc ctccaactgc aaatactagc atcgaaacgg ttgtgaaata
     7141 actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg tggcaaattc
     7201 catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga tcgacatgac
     7261 gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc cttttgttga
     7321 taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt gtactgacat
     7381 cgtatttacg atatttccat ttccttggac tgcccaaccg ccagcaatca ggattgcttg
     7441 aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg catagtatgc
     7501 cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt tactatcctt
     7561 gaaggctgac cctaattgtc caacgatcaa tgcgtatgga ataaaataaa tggccaaaat
     7621 caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa ctacattccc
     7681 catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta attgtttgtt
     7741 gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc atattcaatt
     7801 tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt aatacacatc
     7861 gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc atttacctta
     7921 ttgaatttca agctattttg aattttctgc aaaattaaaa acattagtta ttttataatc
     7981 ctatctattt tctttacatt tttcattaag aaaaggatat aaaaaaaaga ggacgttcat
     8041 tcgtcctctt cattgatcaa cgtttatttt ttacatgcaa gcttcgcatg gcattcaaaa
     8101 cagctaatac tgtcacccca acatctgcaa atactgcatc tcccattgat gcaaaaccaa
     8161 aagcacctaa ggcaagtacc aaaattttta tcccaatagc aaagacaatg ttctgtttga
     8221 cgattttcaa tgttttcttt gctaaatgga ttgcttctgc aatcttcgat ggttgatcat
     8281 tcatgatgac tacatctgct gcttcaattg cagcatcgct tcccaatcca cccattgcga
     8341 ttcctacatc tgctcttgct agcaccggtg catcgttgat tccatctccg acaaatgcgg
     8401 tctttttatt gtttctttgt aagatttcct ctaaccgctg cactttatct tctggaagca
     8461 attcgctata tatcttgtcc acgcctactt gcttcccgat atgatcagca atttttttcg
     8521 aatcgcccgt caacatgact gtgttcttca caccagcctt ttttaattga accaatgcgt
     8581 ctacggcatc ttctttcaag gtatctgcaa taacaagata accactgtaa ctttgatcga
     8641 tcgctagata aagcaatgta ccgatttctt gacttgaagg aaaagagatt tcaaatcttt
     8701 ccatcaattt gtgattacct gctaatactt gtttgccatc aacttcagct aagatccctt
     8761 cacctgcaaa ttcttggata ttagctgttg gtaaaagcgg acctgcgtag ccttctaaaa
     8821 cagattgagc aatcggatgt gtagaaaact gttctgcact cgcaacatat tgcatgaata
     8881 tctttggatc gatttttgta tcgatcgtct ggatcgaaaa gtcacctttc gttaatgtgc
     8941 cggttttatc gaataccact gtctcagttt gtgcaagcaa ctccaagtaa tttgaacctt
     9001 ttactaatac accgatttta cttgctcctc cgataccgcc aaagaaacta aggggtacgg
     9061 agatcactaa tgcacatgga catgagatca ctaaaaatgt caatgctcga tagatccatt
     9121 cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac agctaataaa acggccaatc
     9181 ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat gaaattttca gctggtgctt
     9241 ttttactgct ggcattttct actaattcta ataatttgct gatagtagaa ttttcataag
     9301 atttttctac ttgaactaaa aatggtttat ttttattgat aaatccactt aatactgttt
     9361 caccaacata tacagattta gggactgatt ctcctgtaag tgctgacgta tcgactaatg
     9421 attctccttc gatgatcgtt ccatctaacg gtacacgttc accaggcttc acaagtacaa
     9481 actgaccgat ctgcacgtct tcagggttca cggtcatcaa tccttccgtc gtctgcacgt
     9541 gagctgtttc tggacggatc gccatcaatt cgcgaatcga ttttcttgaa tgactgactg
     9601 ccaatccttg gaaatactcg ccgatttgat aaaatagcat cactgctacg gcttctggat
     9661 attcaccgat caacatagcg ccaatcgtag caacagacat aagaaaattc tcatcaaaga
     9721 tttttccttt gacgatattc atcactgcct tcttcactac atcatatcct atcagcagat
     9781 aaatcaataa ataccctatc aagcgaattg gttcattaaa atcaaataat agcaataaag
     9841 cgatacctgc aagggaaaat aaaatccgga acagcgtatt tctctctttt tcatttccat
     9901 gttcagatac tttttcctta tctatttctg ttactttgac atctggttct aattccttca
     9961 ctactttttt tatttcttgt tcaatcgtag ccaaatgatc ttcctctgcg tcaatcgtta
    10021 gttttccaga agtgaagttt accgttgctt ccttgactcc attgatcttc tgaactcctt
    10081 tttcgatttt cattgcacag ttcgcacaat ctaaaccttc taatctgtaa cttttcattt
    10141 taatgacctc ctcgcctcaa catatgaata tctcttcata actaattata aatgaacggt
    10201 tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac atctgcatcg gaaaatttct
    10261 tccatacaga tgttttggct tattctttct ttttcttttc gatcattgcc actgctctta
    10321 tccaacccgc acttgccttc ttgatcttga caggatagca actaagtttg aagcctgttg
    10381 ctggcaattt gtctaaattc attaattttt cgataggaca atatcttttt tcaatccctg
    10441 caaaatgtcc ttcccagata ttcgaagctc cactacgctt tcaaaaatat agaaagagtt
    10501 ctttaatgaa aattatttca actttccata aacagtaacc ccatataaat aaaagacgta
    10561 aaagtcacat ggaagaactt atacaggaga acgcatagag tcaggtgaaa tacatgtatc
    10621 ccacctgact ctatgcttta tctaaccttt attattctct tttatacaat ttctgcaccg
    10681 aatggcatca acgccaatcg agcaagtttg aaagattgct tggcaaaagg tatccctatg
    10741 atcgtgatac atagaaggat gccgctgacg acatgtgcga gagcaattgg taggcctgaa
    10801 acgatcagcc agatgatatt aacaatcaat gacatgccag actggtgata aattacttct
    10861 ttgccaaaag gcgcaaaaga aagggaggcg atcttaaaac attgcatacc gattgggata
    10921 cctatgatcg tgatacacca aagtatcccc gctgctaacc agctaagacc accgattagt
    10981 ccgccgaata taaaccagat gacatttcct aaacaactca ttgtatttct ccttctatta
    11041 ctgtttttga ttaaaactgg gcaaaaaatg attttattct tcttttttta gcttccagta
    11101 tcaaatattc tgcaaatttt ttttggattt cttccgtatt ttcttttgtt actaatgtgt
    11161 gttggattag ttttcctgat ggtcgctgat ccaatcgttc ttctgactgt tgcacgtccg
    11221 cttgcgcaaa tccgataatc cgtttttctt ttgttacagc aacgagcaat agtttattct
    11281 tatcatttag gtcagctgcc gtttttgctt ttacgaatga ttctggatga tcagtaatca
    11341 gctgattgat ttgaggtaga tcttctttta ctgcccaccg tatttccatc atttctcact
    11401 tttctttcgc tcatttttat ctccactaaa aaaatcgact gcggcaattg catctatcca
    11461 gctttgtgca atgatctcat gtccgccaat cgttgggtgg accccatctt ctcttgtata
    11521 tttcgaaaaa ccataaacaa tccctaaagc attgaatata ccatctaaag gaaccagcaa
    11581 tgcgccatat tccttagcaa gtttacgaac aatttggatt ttagggtcta agtctttacg
    11641 ccagccatga cggtcgtcgg ttataggaag tacaaacggc tccatcaaga tgatgttgca
    11701 gattcccact gtttgaagtt catttagtaa ttttcgataa acggattcaa attgctgagc
    11761 agatttctca gttccgaata tagcctgatc gctagtatta tgccaggtat cattgattcc
    11821 gatcaaaatc gatacatagt caggttgtaa agctaaacaa tcttcttgcc agcgtacttg
    11881 cagatctgca attttgtctc ctcctatccc acggttcatg cattcaaatt ccatcgttgg
    11941 atattgggag attagttttc cggcaatcaa aagtggataa cctttaccta gatcagttgc
    12001 ttctgctcgg tctcttcctg cgtctgtaat gctatcaccg ataaataata tacgtttttt
    12061 catttgtcaa aaactccttt ttgaataaaa cgatgatttt cttccttata ttatcccata
    12121 agccctatca agacgaaaga gaaaatatat gtataaaaaa tgacaccagc ctagtccctt
    12181 caaacaattg aatgaacgct catcaagtgt ctagtttttt atgctatttt tttcttaacg
    12241 tgccggattc cccttgattc cttcgttgtt tggtccagag agatcgatca cacctaagct
    12301 tccgtcaatc agaccactga tgacgccatc agaacatata tttataggct catctaagac
    12361 ttctagtaaa tgtcccttta ctaatatctc ttctagattg cttgggatag attggtcata
    12421 actttatctg gtccgatgat ttcaagggtg aattgaactt acatatggat tattgcatgc
    12481 tgaaaatacg gacctactcc atcttcgaat gttactatta gtttgttttc aggtgctaga
    12541 tgctgcttta tttcaacagc agaagcatca tcaaatcaga tttccatttg aattaccttc
    12601 tttgtaaatg tttgattgat tgcgatttcg ttatagaacc ttgtgaatca aatagcaatt
    12661 tcttttgctt tcttctcttg tctgctggaa acttccttta tcccgtattt taaaaaaaga
    12721 actgcgagag ttcgcagttc ttttttagaa taaacggtgc tctccggcca actcctcggt
    12781 cacaagtcac aatagtcatc aaacatgaaa aactgttttt tgaaattact ccttgtgact
    12841 cggagctgaa cggcaggcgc acaacctctt gatataaacg gtgttcaatc agctgaccct
    12901 cgatattagc taaaaaaacg gtaaaatatg agaagctatt tttccgtttt gtttctctaa
    12961 tactcaggtc aaggcactga tttcacaacc tcttccttaa acggtgttca aaagccagtt
    13021 tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc catttccaca aaatttttct
    13081 taatactcga aactaaacag ctttttcaca acctctccac taaacggtat tcaaaagctt
    13141 acatctgcgg taataagtcc cgccaattca aaaacatgaa cagctgtttt tgcttggctg
    13201 ttctaattac ttgatgcaga acagcttttt catcacctct tatggataaa ttcggttcaa
    13261 caaacgtggg aatggaatag cttcacgcac atggtcgata ccagcaatcc aagtgactgt
    13321 tcgttcaagg cctagaccaa aaccagagtg cggaacagaa ccgtatttac gtagatcaag
    13381 ataccatgaa tattcttttt catccaaacc gtgattacgg atttgttcta gcaagaaatc
    13441 atagtccgtt gcccgttcgc ttccgccaat aatttcaccg taaccttctg gtgcgatcat
    13501 atctgcacaa attaccacgt cttctcttgt tggatgtggt ttcatgtaga atggcttgat
    13561 agcttttgga tagtttagga taaagacagg ctgttcgaat gaattcgcaa tgaacgtttc
    13621 atgaggtgaa ccaaagtctt cgccccattc gatgtcatca aatccatttt tcttcagtaa
    13681 ttcgatcgca tcatcataag taatacgagg gaatggcaat tttgtataag tttttaagac
    13741 ttctttgtct cgtcctagta catctaatgc gtattcgcaa tgatctaaaa cattttgtac
    13801 taagaatgct acatattgtt cctgtacttc taaactttct tcttgatgag tgaaagccat
    13861 ttctggttcg atcatccaga attcaatcaa atgacgtcgt gtttttgatt tttcagcacg
    13921 gaaagtcgga ccgaatgaaa agacttttcc gaaagccata gcagcagctt ccatgtataa
    13981 ttggccactt tgagataaat aagctttttg atcaaagtaa tttgtttcga atagatccgt
    14041 agtgccttct ggtgcggaac cagtcaagat cggcggatcg attttgacaa aatgatgttg
    14101 attaaagaat tcatacgttg cacgaatgat ttcatttctg atctgcataa tcgcatgttg
    14161 tcttgaagaa cgtagccata aatgacgatg atccatcaag aagtctgttc cgtgttcttt
    14221 tggtgtgatc ggataatcgt ggctctcgct taccacttcc acatttgtaa ttccgatttc
    14281 ataaccaaat tttgaacggc tgtcttcacg gatttctcca gttactaaca cggatgtttc
    14341 ttggtgcaga tttttagctg tttggaaaac ttcttccggc acttcgcttt ttacaactac
    14401 accttggaaa aaggctgttc cgtctcttaa ttgtaaaaat gcgatttttc cgcttgatcg
    14461 cttatttgcg acccaagcgc cgattgttac tgtttctccc acatggtttt tcgaatcgat
    14521 gatttgaatt tgttccacgt atgactctcc ttttatattc cttgaatttc ttcaggatta
    14581 attttgactt ttactctcaa taaattgctt gattctgcgt accgcttctt ctaacgtagc
    14641 gcgatcagtg gcataactca tgcggacatt ttctggtgcc ccaaagcctt cgccagtcac
    14701 aagcgctacc cctgtttctt ctaaaagatc ttctacccat tgtgtgacat tttcatattt
    14761 acacatgcgt agcgtttctt ttacattagg aaaaagataa aaagctcctt gtggcttttc
    14821 aagagaaaca cccggcagtt ctgccacaag cggatataac gtattcaggc gttcttcaaa
    14881 ggcgttccgc atttcttcca ctgtatcttg ctcgcctttc aatgcttcaa cagctgcata
    14941 ttgactgact gctgtcggat tgctagttga ttgggaagca acagcgatca ttccattgat
    15001 gatcacagga tttcccaccg cataaccaat gcgccagcct gtcatcgcat atgttttaga
    15061 cacgccgttg atgatgatgg tctgattttt gattgcttct gaaatcgttg caattggagt
    15121 aaactcattg ccattataaa ctaaacggcc ataaatatca tcagcaacta tcaaaatatc
    15181 attttttact gcccattcac cgattgcctg cagttcattc ttgctataaa tcattccagt
    15241 cggattcgat ggagaattga tgattacggc ttttgttttt tctgaacgtg cttgttctag
    15301 ctgctcaaca gtaactttcc agctattgga ttcttctcct ttcaaaaaga caggaagtcc
    15361 ttctgccagt tttacttgct caccgtaact gacccagtaa ggcacaggaa tgatgacttc
    15421 atcttgcggg tcaaggatcg tttggaaaag ggtatacaaa gcaaatttcg ctccgtctgt
    15481 tacgactgtt tgtgaaggtt catagacaag tccatagttt ttcttcagat aatcaacgat
    15541 tgcctgtcga agctctggga ttcctgctgt tggtgtataa tagctagctt tcccattgcg
    15601 gattgcttcg attgctgctt cttggatgtt ttctggtgtc gcaaaatccg gttctcccac
    15661 agtcaaactc agcacatctc tgcctttggc ttttaatgcc ttggcctttg ccgcagctgc
    15721 caaagtgaca gacggttcta atcgttttgt tctttttgat aactccatcc cattcattcc
    15781 tttacctttt aaattccctt gattgtcttg acttcatctc cagattcaaa gccaatcagg
    15841 taataattca gtttcccatt tgtttctttt cctactactt cccacacagg ctgatcatca
    15901 tacatcccta aagcagcttt ttcgatttgg atttcaggat gggccgcagc aattgtctgt
    15961 tttgcttcat tttcagtcag tccctttgtt tgatccaata cttttacttt ttcaccagat
    16021 ttaggaatga taacggcgat ttcttttcct ttatcatttg tacctgtcag tgaaaagtat
    16081 gtcttctttc gagtaaacca ataaaaatga tcggcttcct caagatttgc atatttcttt
    16141 gcgatttcta ctgcttcttt cttcgcctgc accgccggac gactggaacg caagtaaaaa
    16201 atcgagctga tcacgatgac cgctaataaa aatacgatca agccaagtaa cacgcgatta
    16261 atcgtttttt cctttgtctg ttttttcttc aagggtcgtc gctcctttat cttcattcct
    16321 tttcattata gcagatttta attatgaaaa aagacagctc ctctaacttc cttcttcatt
    16381 tttctttaag aattcatgga tatcctgata gatttcctcc atagctagct ctttgatcgg
    16441 tacttttttc ggtaatgcat ggatgatccg tgcaccatat tttgcagtaa ctagtcttcg
    16501 atccaaaata agcaatacgc cacgatctgt ctccgtcctt atcaagcgtc ctaatgcttg
    16561 gcgtaacttc aaagctgctt taggtacgga ttcctggtaa aaaggattga tcccctctga
    16621 ttctaaataa gcgttcctag ctttgaccat cgggcgaagc ggattatcga agggtaaacg
    16681 tgtgactaca acgatctgca aagcatctcc aggcaaatct acaccttccc aaaaactgtc
    16741 agcgccaaac aatacgcttt tttctgacaa aatgaagcgt ttcaaaagct tctcccggct
    16801 tccgccaaac ccttgagcta gtatttctcg tcctttttct agcatagaca gatgcatttt
    16861 ctgatagacc ttctgcaaaa catcatgaga agtgaataac acaagtattg gccgatcttc
    16921 ttgttcgatc agacctgtaa gtgctgtctc taaataatcg atatatgctt gattgctgac
    16981 ttgatggacg gcaggtgcct cttttggcac atagatcctt gcctgtttct cataatcata
    17041 aggagaggca atcactttaa gaggtgctgc ttcaatgcca agccgcttag agagatagtg
    17101 tcgattaggg ccgatcttca gcgttcctcc aatataaaga atacgatcat atcgaggata
    17161 ccaaacagtc gacggcaaca tagccgcttg aaaatcatgg agatgaaaaa tcatctgctg
    17221 cgctgtgttc ccaggaacga accaatggat caaatgatct cgccagtctt ccagccattg
    17281 atccatcagc aagtactgct tgtctaattg atcaaataag ctatataaac ttgctaaatc
    17341 gatccgcatc tggtttgtcc agctttcttg gataatctca aaagtcagac gcatttgtga
    17401 ctgtagctgc tggatctcct gataaaaaag ctgcaggtga tcaatggctg tcccagattc
    17461 cagtggcagc tgatccaaca tctcttttgt cgtcatgact tcttctggat actgtccgtt
    17521 aggttgcttg ccgctcacta aactcattaa tccttctgcc atacaaatct gtgcttcgca
    17581 tagcgcagaa agttcttctc gataaatcga taaccagcgt aaggcttcat gttctttcgg
    17641 taaatagctt tgtattcggt caaacagctg atttggttct tctaattgct ggatttgttt
    17701 atggaaatag ttaatactta atcgtttgtc atttactttt tcagcgattt ccggcaaatg
    17761 atgtacttca tcgatcaaca aaaatggact agctggaagc aatggttctt ttcgctgtgt
    17821 ttcttgtatt aaaaacgcat gattgacaat caaaaaatta ctttgcctga ttttcttttg
    17881 caagaaacga acaaagtctt gttcgtaaaa tgactgattt cttgctaaaa atccagtgcc
    17941 tctgtgcgcg atatctgcaa agaatgaatg gttcaaattt gttaaatgta attcatccaa
    18001 atcaccggtc ttcgtttgcg tcagccaaac taagaccgcc atttgataca aagcatattg
    18061 tttttgttct ggcggttcca caagtgtgcc tttaaaacgc tgtaaatcaa tatagtggcg
    18121 atagcttttg ataattacag gttgtatggg ctgatctagc aattgattca gcaatggaat
    18181 gtctttttct atgatctgct gctgcaaaac tagcgaaacg gtactgatga cagcgggttt
    18241 ttcaggtgta gccaaataac tcaatggcag cagataaccg attgttttac ccattcctgt
    18301 agctgcttcg atcatcaaat cttttgattc atcttttgtg aaatggtcgt agaccgcatt
    18361 catcagtctg ttctgttctt ttcgatacat cagtgtttta ccaaacattt tttcttttgc
    18421 ttgttttttt cttggatatg tccgctcgcc ataatggacg tatatgaata gttccacttc
    18481 ttttttctgc aaagctagcc catcgacgat ttctaatgag ggatcaagtg gctctggttt
    18541 ttctttcatt tcttcaatca catgataaat gaaacgactc gtatccatac ctgtgtgtct
    18601 acttaatcgc gcaatctgct ctaacgtgat gattggcaat tttctcatcc gttcttcgat
    18661 cagcaaaaga agttgacccg tcacttcggc atcactatct gcctgatgag gattctcatg
    18721 aataaatcca aggctttctg acaaatcagc taagcgaaag cttggttccg tcggcaagaa
    18781 aatttgtgca agttctactg tatcgatgcc tggtatcttc aaactaggaa gtccgcatcg
    18841 catgagttca tggttcaaaa aattataatc aaaataaata ttatgcgcga caaatatggt
    18901 gtctgccaaa agattgtaaa tcgtttgggc gacatcttcg aaataaggcg ctttttggac
    18961 gcgttgattc gtgatccctg ttaaatgctg gatttgtttt gaaatcttcc gccctggatg
    19021 aatatcgatc gaaaaacgtg aagtgatccg acctgattca atcatgacgc agccaaattg
    19081 gatgatccga tcttcttttg gattcgtccc agtcgtttcg atatcaacga ctgcataagt
    19141 tcttggtttc aaaatatctt cctcttctcc tgcctctaat gttcttttga aattatacta
    19201 caaagtgcag aaaaatactc tcatcgcttt ttaaagaaaa tagacaaaaa cgcccagaca
    19261 cgcaaatgtc tgggcgttta ttttttagta taggttaatt aagctttagt aacgtttgtt
    19321 gcttgaggtc cgcgttggcc ttcttcaaca tcgaaagtta ctgcttggcc ttcttctaaa
    19381 gttttgaatc cgtcgccttg gatagctgag aaatgaacga atacatcgtt tccgttttct
    19441 gcagtgatga atccaaatcc tttttctgag ttaaaccatt ttactgtacc tgtttccata
    19501 ataaaaatcc tccttgtgta tcaacacata ttttgcaatt acttgaatgg tgtatatctg
    19561 gaagatgttt atttgaaaca agctaccaca gattaccgaa caaaaactac tcctatagta
    19621 tatcataccg tttacaaaaa agctagagtt tttcgacttt ttttattaaa aaaagttttc
    19681 tcagcgatta cataaagtga aaaaccgaac tttttttaaa ataaacgaca tattgttcag
    19741 ttggtgtttt tttgctctga tgttcttttt taaactaaaa aaattattct ttctgactac
    19801 ttatcggttc tgtttcaacg gtcgaggctg gggaagattc tttagtaggt ctgatgttct
    19861 catggtattt ctgatagcct atgacagtta ccagaagaac aaggcaaacc aatacgatca
    19921 acgagattag tttttctttt ttgctcatat ctatacctca taaataacag gatgatgtcc
    19981 atcatatttc tgagtatgag taaaagcaaa agctgcagct gtagaatgta agaaatgcca
    20041 gcaactttca taatcacagg tcggacagta ccaaaagtaa ccaaatcttg gctgcaaaga
    20101 ccgttcttct gtttcaggaa tcgcagaatc aagatctggg gtctgtacat aaacattttt
    20161 gtacagacgt gtgcctgtaa taggagaccg ctgtgtttct tcagcaaaaa ctggctgact
    20221 tgttaaactt agggatagaa acagcaaaca ccataacagt ctttttttca taaagacctc
    20281 cttatcgata agtttttccc aattaaaatc atatctcaag aacgcccgtt attcacattt
    20341 tgttcataaa tgatgttttt tgtatcataa tcgtcaaaaa gaggtcgtga ccacgtcaca
    20401 acctcttttt taaacggtgt tcaaaagctt gcatctgcgg taataagccc agaaaaacga
    20461 aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt
    20521 cacaacctct catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga
    20581 aaaatatgtg aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt
    20641 cacaacctct tttaataacg aagaattgct gtcagactct tttcatctgg cgcatctttt
    20701 tgatccaaaa taaacacttt tccgccatta atgatcacat catctgctaa tgcattcaac
    20761 acttgacgcc gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac
    20821 gtagcgataa ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac
    20881 tgatcgatga atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt
    20941 tcacttgaca gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa
    21001 gcagaaatcg caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc
    21061 tgattttctg gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc
    21121 ttcaagtacg tatcaactgc ttggtaatag ttgacccaat cgatctctac ttcttcatct
    21181 tttggattta cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag
    21241 aagttcaaac cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc
    21301 ccttctggta gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag
    21361 tcgcggttca atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc
    21421 agtaaataag gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg
    21481 atataggttt ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac
    21541 gaaggatctc ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa
    21601 tattttttct caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct
    21661 ttttccacat cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta
    21721 gcgccttctg aagtcaattg tgacaataat tctttatcct tttttgccat aaacgtaccc
    21781 ctcctaaaaa aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat
    21841 acaataaaac gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt
    21901 ttaagttatc ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga
    21961 aatgctgata aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt
    22021 ttcagtttat taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg
    22081 gaaacacagc ctattcctgt aaaagtacgt aaaaacgacc gctaaccttc cataatgtgc
    22141 gttcttatcc taaagttttt tcagaaacat tgaaactctc ttccctctct gataaaattt
    22201 taatttgtag cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg
    22261 tctagtccta ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc
    22321 tgtgcaagct gaaaatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca
    22381 aagcggtaag attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac
    22441 aaaaatcatc ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tatcatggga
    22501 tgataaagtt tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa
    22561 tgttcctttg cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat
    22621 ccagtcagcc aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa
    22681 attcactgaa cgaatgaaag aacaattgaa agattgggga atcaaagatg caacgatcgt
    22741 taatgcatca ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga
    22801 aaacgacgaa aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt
    22861 ggatttccca gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac
    22921 gcaatcacca gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa
    22981 ggaaggcgtg gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg
    23041 tacgatcact aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc
    23101 ggaaaacccg agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa
    23161 ttggtcagaa aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga
    23221 tgtaaaagac ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg
    23281 ggtacgaaac gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca
    23341 agtcactgac aacgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac
    23401 gatcaccttg gcagacgata aattaggtta tttagaagac aacaatgcgc catcgacaga
    23461 gatcattacg gataaaacag tggaaaaagc gaatatgttc gtgattggct ggcgacgcgt
    23521 cgcagaattt ttcggcaact tgttttaatt gaacaataag aaaagcatct ttccatcgga
    23581 aagatgtttt tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata
    23641 ctgtataaga aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc
    23701 tctatcttat tggggtattc tctatctttt attggcgaaa acagaagaag gatccttcct
    23761 tgttctttta cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg
    23821 aaatttttta ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt
    23881 tttcttattt cacagaaaaa cgaaggctgc tgaatggtct tttattcaat gttttcctca
    23941 tcagtttcgg catctacttg tttgtcttgc tgtatgaaac gcaaaatatt ttccttggcg
    24001 gactgatcgc cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat
    24061 tgattgtttt tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag
    24121 ctaatctttt aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt
    24181 tcttgttaaa atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct
    24241 tgatctatct atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca
    24301 accggcctcg atacaatcaa gacttcatcg ttgtgttagg cgctgggttg atcaatggag
    24361 aaacagtttc tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc
    24421 aaagccgtgc tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg
    24481 aaaaagtacc agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa
    24541 gagacattct tgtcgaaacc aattcgacaa ccactttgga gaacatgctc tattcaaagg
    24601 aaatcatgga tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc
    24661 acatttttag agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg
    24721 caaaaacagc tttctactac ttgcctaatg cgtttttaag agaatacatc gccatcttga
    24781 tgatgaacaa aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct
    24841 tatcgctgat tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct
    24901 tgtttttttc tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta
    24961 cttgctgagc agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca
    25021 taaagaagta tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa
    25081 tcggtgtatt tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa
    25141 cagaaacatg gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg
    25201 gtgtcacttc tcccccttga taccccgcgc ctaaagacaa aactgtgaag aataacttcc
    25261 caataaaatc atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca
    25321 atccgagata acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta
    25381 tcgtatttcg ccaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga
    25441 tcgaacggct gaatacccag ccaatcaaac caaaagcaat cccagcaaca aaaactttga
    25501 agaacagcaa catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac
    25561 catagctttc tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt
    25621 ctgttcggac tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg
    25681 tgccaaatac agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg
    25741 atttatccaa tcggaacaaa cgtgctgtat tatctgctaa cgctcccccc atttggactg
    25801 ctgttccttc acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg
    25861 taagtggaat aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta
    25921 agttgtttcc acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac
    25981 cactaactgg tagtagataa aggagccaag gatgtgatag tcggatatct gtcacaactg
    26041 tcaggctatt taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga
    26101 tcaccagcca cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca
    26161 taaaaaattc ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt
    26221 agtaggcgtc attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac
    26281 ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta
    26341 cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac
    26401 tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg
    26461 tagaaagaaa aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta
    26521 tcgttggtga taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga
    26581 attttttctg cgtacctgga tcattccagc cgatgtacgt atgtgcttgc aagtcgattt
    26641 cattccaagc tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt
    26701 tgctaggctg cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt
    26761 gcgcttttgt cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt tctggcagtt
    26821 cgtgatctaa tatgtctcga atcttttcag ctgctagtgc ctgaactgtt tgtaccgttt
    26881 ttggatcatt gacagatgag tacgcattta ataactgttc tacttctctt attataaaaa
    26941 aatattggta tggttttatt gtttgttcca tgattttaaa gtcctttctt ctaaccaaag
    27001 tgcgtacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga agatcccgga
    27061 ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgc ttattattct
    27121 atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgaacagat agtcaactgc
    27181 aaacagaaaa aaggactgtg acaaaagtcg tgtcaaagtc ccttgtttcg aatgaatggt
    27241 gttcaaaagc tggcctctgc ggtaataagc cgaaacttca caaaaatttg agaagcaatt
    27301 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct caatatggtc
    27361 tattattggt tttacctatt aaatcgagga ttctttccca aagtaacgtt gccatctctt
    27421 acgcatactt gcagacatgg gctgagccgc ttctttgact gcgcagtatt tgtccacgaa
    27481 tgtcacgata tatccttctt tgtacttagg cggtgcgatc gttgcacccc acatgtgctt
    27541 caaaatgata tctcgctcaa gatcagacaa atcggtgatt ttttctgcat tctttacagc
    27601 aattcttgga tggatatacg catgtgtccc ctcgtcaaat ttcgtcgtac gccaatcata
    27661 gtagaacaga tcatgcaaaa gtccagcacg cgctgttgct ttagcattgc caccccattt
    27721 ttttgcgagc agataactat agtaagaaac actgatggaa tgctccaaac gagtggaatg
    27781 aacatgctgt gtgtaattcg ctaatttttt cactgcttct gtttctaaaa gatcttgtac
    27841 ataagacata tattcttcgt cttcacgcca tttttctgtc ataattatcg atctgccacc
    27901 tttttgaatt tcattttctt ctgaaattga tctaagtata ccaccagtgt tcggattatt
    27961 ctagtcgctc tagataaaat agctaaagct taatagttcc ttaatcataa agaaaaatga
    28021 taaagaacta tccctgctgc gactccgaca ttcaatgatt ccgcatttcc tttcattggg
    28081 atatatatgt tctgatcagt caaagacaag atttcctgac ttactccctg accttcgttt
    28141 cccatgatta tagcgaaatc ctctgttttt tctactttgt ctaatgcatg tgcttcttca
    28201 tttaattctg taccgtacac cgtaaaccct ttgcctttca ttacagggat cacttctgcc
    28261 aaatcttttt gtaacacagg caaatgataa ttgcttccct gcatgcttcg cagcaccttt
    28321 gtactataaa tatctgccgt tcctttacct aataaaacac cagccatacc aaacgcatct
    28381 gccgtacgga tcatcgttcc cacatttcca ggatcctgta cattatctag taaaagccag
    28441 cctcctgaaa aatctggcgt atctttagat gttaacgtca tcactgctaa gatcccttgc
    28501 ggtgtaggta gatctgacat agcggtcatg acctcatctg aaactagaaa agtagaaatc
    28561 tctttgttta cagccagcca gtctccccat tctttcatac ctctctgtgt cacaaaaagt
    28621 tcttttattt ctgcatcaga cgacgcagct tcttcgatca gatggaaccc ttccaaaaga
    28681 tattctgcct tttcttctct gtatttcttt ttatgtagtt tttttaattc tttgattctt
    28741 ggattttttg ctgattggat ctctttcaca ttcctcacct caactgatta tatcatggaa
    28801 taggtgcttg agcttgtttc atcctaaaaa aatcggtatc attgatttat aaaacaggaa
    28861 agaaaggtga cgaacatgcg caaagttaaa atgaatgtcc aaggacgtgt ccagggcgtg
    28921 ggatttcgtt atatgaccaa gatggttgct gatcagttag gcgtgaccgg aacggtaaaa
    28981 aacgaagacg acggctcagt cacaatcgaa gcaatcggaa atgacgacat catccaaaaa
    29041 tttatcgaag aagtcaagaa gtctccgagc ccaagtggac gagtgcagta tgtggatgtc
    29101 caagaagatc cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtttt
    29161 tgaagcgctt tgcctcgagt attaaggaga aacattttcc aaatagcttc tcatattttg
    29221 aaatgtaatg acttaatacc gagaggcagc tgataaaaca ccgtcaataa ggttgtgaaa
    29281 aagctggtct gcatcaagca ttaaggactg gttgtgaaaa atagctctcc atatttttca
    29341 acaggctgga cttaatgccg tagatgcaag cttttgaaca ccgtcgataa ggttgcgaaa
    29401 aagtcgctta gttttgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc
    29461 ataaatttga cttaatacca agaaattgac ttttgagcac cgtcgatcag gttgtttttg
    29521 aggcgttttg cctcaagtat taaagatagg tagtaaaaaa gctgatctat aacttgcaat
    29581 aaatttgctt aacccatatt ttccagacag aacaaaaaag tttttagttt tttaaataag
    29641 aaagaaacac ttggaatcac tcttttcttt ctaagtattt ctttcttgat tttttgtctt
    29701 ttttcaaaat ttttcgctac gaaaaagaac gaatatttat atgaaatttc tatgaagttg
    29761 attgaattcc atgcgctttt ttagtatagt taagtttgtg taaaaattat agaagaggaa
    29821 gtattctatg aataaatgga aaaactggtt attgggatcc ggacttgtca gtatcctgct
    29881 ctttttatct ggctgtgtgc ggatggattc aaacggaaac cctgacactt caggaatcgt
    29941 ttatcgagta ctcgtccatc ctatgggcca agcgattaca tatctagttg agaatttcaa
    30001 ttggtcttac ggctgggcag ttattgcaat gactgtgatc gttcgtatca tcatcttgcc
    30061 actaggtatc agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc aagcgttaaa
    30121 accacaagtt gaagcagcac aacaaaagct aaaagctgca acaacacgtg aagaacaaat
    30181 ggcagcacaa gcagaaatgc agcaagtata tcgcgaaaac ggattgagta tgaccggcgg
    30241 aatcggctgt ctgcctctat tgatccaaat gccgatcttc tctgccttgt attttacggc
    30301 tcgctacaca gaaggtatcc gtgaatcatc tttttacggc atcgatctag gctcaccaag
    30361 tatggtgctt gtggccatcg caggggtcgc ttatctgctt caaggatata tttcaacaat
    30421 cggtattcct gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct ctcctgctat
    30481 gatcgtcttc atgtcgatca gtgcgccagc cggtgttaca ctttactggg tagtcggtgg
    30541 tgtgttcagc tgtctgcaaa cattcatcac aaacgttatg atgaaaccac gtttgaaagc
    30601 acaagtggca gaagagttga aacaaaaccc gccaaaacaa gtcgtcactc caagaaaaga
    30661 cgtgacagaa tcagcaaagc cagctgacaa caaacaagca acaaaacaaa tcaaacaacc
    30721 aaaaaacagt acaaacaaca aaggccgcaa cgcaggaaag cagcagaagc ggaagtagat
    30781 agtaaataaa agtgtaataa aaattcatta acagttttag caattaaaaa tatcgaccaa
    30841 gatttttgaa aaagaaatct ggtcgatatt tttatttaag tgaatgttta atttgaatca
    30901 tcgattgatc gctatctaat tgcagcagca tctttttatg aagaaacaac tttttcttat
    30961 ctatcaacgt agacaggtgt tgctgtttct tttaataatg tacttaattc cttaatattt
    31021 tgttttcctt cttctaacat ccaactactc tctttttttt cgccttcggt aacaaaaggt
    31081 gccactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    31141 tgttttgcaa aacgtaatgt atcttgaaat agcgtagcat tgactcccgc acttaaaaaa
    31201 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    31261 ctataacaag tctcttgccc atattcttcc acaaatctca tattcactgg cacttctact
    31321 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aataacttta
    31381 tggggtttta cttttgcata ggcaagagag gacggatcgc caatagttgc atcataagaa
    31441 aggatctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    31501 actatttgtt tttgcacatt aatctctgct ggttcatcca cgtcatagta caataaaaat
    31561 ttacaagcat ccgcaccagc ttcttttaac cttttcaccg accatatatt caataaatca
    31621 ggtaatcttc ccggcatttt cgtatcatag ccggtttttt cataagctag cagcaagcca
    31681 cattctttct ctcgaagcgc tgctgcaggc aggccgtatt ctggatcaag taaaatagct
    31741 gaggcatagg gagtcaggta ttgagaaacc atctgtttaa attggatgat gtcttctgat
    31801 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgctggtc gattactaac
    31861 gcgctaataa ttccagctta atccgtcaat cgcttcaaac gttcctgctt ttctatactc
    31921 atcttcttca tatatcttca cctatttctt tagttaatat tgtggctatt tttgtttttg
    31981 attttgcttt tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    32041 acaataattt caaatgagtc gttcctgctt ctgaatctgg aataaataac gcaaaaataa
    32101 aatcaactga ctgtctatct aaactgttcc attcaattcc tttgttcagt gtgacgataa
    32161 tcatactagg ttcgttgatt gtttgagact tcgcatgagg aatagcaaaa cctgacatta
    32221 ttcctgtagt accttctttt tctctttcta gaagtttttg atatacatct ttttcgtcat
    32281 gagcaattcc taagcagaat gtttgctttg ctaaaaactt aaatgcttct tcttgactat
    32341 ccaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata agtctcctgc
    32401 tcttcctttt tcgataactg tacgatttgc tgctttatta tccatcttct tgtctttatc
    32461 agttactctt ttcttttgga ttaatcctag tatcactcca ccaacgatcg aaccaataag
    32521 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccaccatg
    32581 tggtgctgga acttggacgc caaacaaata agttaagaca gcagcaattg atgagcctaa
    32641 catcatcgtg ggtaacactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    32701 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tggtatcaaa
    32761 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tacaagctgc
    32821 tgacacacca gccataaaag tcgtgtttcc ttgtgccaaa agagctgttc ctgttacata
    32881 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacacc
    32941 aagaacaagt ggattcgaat gttcaaatcc agctaaaaag ctcatcattc cttcatttat
    33001 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    33061 tggatataag aagattgcct tcaagccatc tagttgttta ggtaaaattg tcagtagctt
    33121 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    33181 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    33241 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    33301 gccaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcaaaaga
    33361 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    33421 caacatatga gagacaccat tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    33481 actcatcact tctggttcta aagtatcaac agcttcatta tctgctaaga cagtaccttt
    33541 tcctgccaat gcctcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    33601 atcaatgact cgttttcccg caaatctttc tgaatggaca tctttgtctg cggcaataat
    33661 gacagcatcc gcctgtttga tttcttcagc cgttaattca ttttcgatcc caatttgtcc
    33721 atgggtctct actttgatcg tgatgccttt tttcctagct gcttgttcta atgcctcttg
    33781 cgccatgtag gtatgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    33841 tcccatcttt cgaacctctc ttccccgatt ttgatttgtt tacttaattc ctccacatct
    33901 aaaaaatctg ttaatccttt gcgaaaagcg gttgagctgc ctgctgctat gcttttacgt
    33961 aacgtttcat caagcggtcg tcgattcatg tatcctgcta aaaaagcgcc taacatggta
    34021 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctct aaacaccctg
    34081 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaatcatt ttctgcacct
    34141 aactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    34201 aaccaagaag aaagttcttc ctcgttcggt ttcagcaaaa atggtcgata gggtaaacaa
    34261 tccatggctt cttgatcact actatcaata atcaaatgaa tccccttttc ctgacaaatc
    34321 cgactgattt ctattaaaac ctttggagac agccctcttg gtaaactccc agaaacacaa
    34381 agataatctt ctgcttgtaa tactcttatt tgatttaaaa aattatggac agctgtctgc
    34441 ggtatttcag gtccttgatt cactaatttg tattcttctc ccgtttgatt gacttgtgta
    34501 aatacattga tacgcgtcat accaggcact tcaataaaat ctgtttggat ttgtttctgc
    34561 tgtaaatagt cttctatata ctttcctgtg aacccagcag aaaaacccaa tgccgtactt
    34621 gggaaatcta gcatctttaa tactagtgac acattcactc cttttccgtt tgcctgaatg
    34681 tcatcctctt ttgtgcgatt taccataaaa gaatgcatct cctctgtttc aataaataga
    34741 tcaattgcca aattcatcgt gcatgtgtag atagacataa ccaatgacct ccttgactct
    34801 attatgaaat acgcgaaaga gaaaatgttt tcttaatcgt tccaacacaa agaaaacctt
    34861 tacatttata aactcgtcgt gctatttttt aactaacttt tggtgaaaga aggaaagaat
    34921 atggatcaca aattaagtga aacagaacag tacctctgga actttatcga acaccacatc
    34981 ctagagatcc ctaactactc catcgtgaaa ctaagtgaac aagcaaatgt ctcaacagcc
    35041 accattgtgc ggacaatgaa aaagaaaggc tatgaagggt ttacttcgtt taagcatcat
    35101 ctaaaagaag gagaaaacaa gaacatcaat ttttctttct tagataaagt agacaaaggt
    35161 atccgccgag ccattctaaa aaacgaacaa gaagtggtac gtacaatcaa tatgttggaa
    35221 atcggaaata ttgaggatgc gatacaaaga atcaaatttg ctgaccgggt ttttgtcttt
    35281 gcccgaggat tttcagaaat gattggccaa gaaatgctgg taaaacttca attaacagga
    35341 aaaaattgtc aaatgcatac tgatccagag attatcaaaa gcatcagtcg gaaactaact
    35401 aagaaagaca gtgttatttt tgtttctttg aacggcgaga caaaagaact cgtcatcgcc
    35461 gcaaagaatt gctatgatgc agagattggt tcaattctag ttacagccaa tcattcctct
    35521 acactcaaag attattgcga gataaatctc gtaggtttca aatcagaagg ttcttatttc
    35581 cctgattatg aagtacggtc acgtttgcct ctccagatca tcgcgagact cctgcttgac
    35641 gcctatgtat tgcgtatggg ggagaattga ggatatttga tttgcgattg ataacaattg
    35701 ctagaaaaac tgtaaaaaat gctttacgta tctcgctact ataatttatt agtttcattt
    35761 cccattttag ataaaagcta tcgtgtactt tagcatggct aaaaatacta caaaataaat
    35821 agacaaaagg aaataataaa aaaatctgga acagaagctt atgctttctg ttccagatta
    35881 gggttgatga aaattaaatt atttgaggtt catatttttg tacataaaaa ttctaaaaat
    35941 aacttattga aagtcaatcg gcttttcttg ctgtatccca aaattataag caattgcatc
    36001 taatatacgt tcacttgcat taccatcgcc atatggattg gacgcttgcg acattttctc
    36061 gtattcttct ttatcgctta acagcagagt ggcttcttca aaaactttgt cttcttctgt
    36121 tcccaccagt tttaaagtac ccgcctcaat tccttccggt ctttcagtgg tatccctcaa
    36181 gaccaatact ggcacaccaa gagaaggtgc ttcttcttgt acacccccag aatcagataa
    36241 aatcatatag ctgttattag aaaagttttg aaaatcaata acatctaaag gttcaattaa
    36301 gtgcacttgt tcagagtcac ttaatgtttc ttctgcattt tttcttacct taggattttt
    36361 atgcattgga aatacgatat gcacatcttc aaatttctca actaatctat tgattgcttt
    36421 aaaaacattc gtcattggtt gacctaggtt ttcccgacga tgcattgtga ctaaaataat
    36481 tctttttcct gctagattat ctagcaattc attagaataa ttttgtttga tagtgtattc
    36541 catggcgtct atcactgtat ttcctgtaat aaatatagat tcctctggtc ttccttcctt
    36601 gagaagatta gatttactca tcactgtagg tgcaaaataa atatctgcta gtgtatcagt
    36661 caattgtcta ttcatttctt caggaaatgg agaatattta ttccacgttc tcaatccagc
    36721 ttctacatgg ccaatcttta tttgatgata aaatcctgcg atagatgctg caaatgttgt
    36781 tgtcgtatca ccatggacca aaataatatc aggtttagct tctaccaata cgctgtctaa
    36841 ctctttgatt actcggctcg taatatccgt caatgtttgt ccatctttca tgatatttaa
    36901 atcataatct gctttaagat caaaaatatc cattacttga tctaacattt gccgatgttg
    36961 tgctgtgact gttacgattg attcaaagcg ttcatcattt tcaattgctt ttatcaatgg
    37021 agccattttt attgcttcgg gacgagtgcc gaaaatggtc atgattttta ttttcatttt
    37081 taactcctaa ctaaagtcat aatttattgg atttttcctg atatcttgaa tagttctcaa
    37141 agtatccctc tgaataacca tactaacaaa caacaatatg atgtttaata gaataatata
    37201 ttttaaatta taggaattca ttatacccgc aagtaagggt agcgtcaaga attatgtgta
    37261 aatggaaaat ccattccgtt aattacagtt aaatcattga ctctaatgta tcctgaattt
    37321 ctttaaatcc tttgtgaatc ctgcctaaaa atttttggtt atattgatta aattgcgaca
    37381 ctaaaaatct ttctaaagag tcttcatttg gaaattgttc ttttcttcaa gtataccgtt
    37441 ttaattgttt attaaatcct tcgattaagt tcgttgaata gatggttcgt ctgatagtgg
    37501 gaggaaaatt ataaaatgtg actatggcag gattcaagag cagattgaca actcttggat
    37561 actgctttcc ccattttttg accataaagt ccacttgagc caatgcctct tctttagacg
    37621 ttgcttggta aaccgctttg aactcctcac aaatttcttt tcgatctcga atacgcactt
    37681 tatgtgcgat atttctagat acatgtacac agcattgttg aaattgtgca ttcggataca
    37741 cactatggat actttcttcg ataccactta aaccatcggt taccactaat aaaacctctt
    37801 ctaacccacg gtttttaaga tcttgaagaa cctctttcca aatatacgcg gattccgttg
    37861 gggcgatagt gaacccaaga acttctttgg ttccatccaa tcggatacca atcgtaatgt
    37921 acacggcttc ttttgaaacg gtttgtctct tcaaaggaat atgtgtcgca tccataaaga
    37981 taacggaata actggcttct aacgttcttt ctttaaatgc taaaacgtct tcagacacga
    38041 gtttactcat gttggagatc gtttgtggcg tatagtgatg cccatacatt ttttcaatta
    38101 attcggaaat ttcagccatc gtaatgcctt tttgaaataa ttggataacg gtcgtttcta
    38161 acgaatcatt ggttctctta tatgctggga gtgtttgttt agaaaattcg ccattccgat
    38221 ctctaggaat agttaaattt agctctccat attctgtttt aaatgtacgt gaataattcc
    38281 cgttacggga atttcctgaa ttgaatctat ttcgatcata tttttcgtag tctaaaaaag
    38341 cagttagctc tgcttgtaga agtgaattaa tcgctcgttc taaatgatcc cgaaataatt
    38401 catctaaatc gcctttattg agtagtgttt gca
//
LOCUS       DAEGIQ010000015        35575 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000015 DAEGIQ010000000
VERSION     DAEGIQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.015"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            256..2022
                     /locus_tag="KMT45_000703"
     CDS             256..2022
                     /locus_tag="KMT45_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBE7004186.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2124..3383
                     /locus_tag="KMT45_000704"
     CDS             2124..3383
                     /locus_tag="KMT45_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBE7004187.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3530..4831
                     /locus_tag="KMT45_000705"
     CDS             3530..4831
                     /locus_tag="KMT45_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7004188.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4834..5685
                     /locus_tag="KMT45_000706"
     CDS             4834..5685
                     /locus_tag="KMT45_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7004189.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5698..6513
                     /locus_tag="KMT45_000707"
     CDS             5698..6513
                     /locus_tag="KMT45_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBE7004190.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6664..7830
                     /locus_tag="KMT45_000708"
     CDS             6664..7830
                     /locus_tag="KMT45_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE7004191.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8060..8707
                     /locus_tag="KMT45_000709"
     CDS             8060..8707
                     /locus_tag="KMT45_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBE7004192.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8791..9735
                     /locus_tag="KMT45_000710"
     CDS             8791..9735
                     /locus_tag="KMT45_000710"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBE7004193.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9870..11321
                     /locus_tag="KMT45_000711"
     CDS             9870..11321
                     /locus_tag="KMT45_000711"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBE7004194.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11342..12178
                     /locus_tag="KMT45_000712"
     CDS             11342..12178
                     /locus_tag="KMT45_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBE7004195.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12178..12837
                     /locus_tag="KMT45_000713"
     CDS             12178..12837
                     /locus_tag="KMT45_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBE7004196.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            12963..13652
                     /locus_tag="KMT45_000714"
     CDS             12963..13652
                     /locus_tag="KMT45_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004197.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13658..14614
                     /locus_tag="KMT45_000715"
     CDS             13658..14614
                     /locus_tag="KMT45_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBE7004198.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14658..15170
                     /locus_tag="KMT45_000716"
     CDS             14658..15170
                     /locus_tag="KMT45_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7004199.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15426..15974)
                     /locus_tag="KMT45_000717"
     CDS             complement(15426..15974)
                     /locus_tag="KMT45_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBE7004200.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16063..17244)
                     /locus_tag="KMT45_000718"
     CDS             complement(16063..17244)
                     /locus_tag="KMT45_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBE7004201.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17480..18988)
                     /locus_tag="KMT45_000719"
     CDS             complement(17480..18988)
                     /locus_tag="KMT45_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBE7004202.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19147..19338
                     /locus_tag="KMT45_000720"
     CDS             19147..19338
                     /locus_tag="KMT45_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004203.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19541..20458
                     /locus_tag="KMT45_000721"
     CDS             19541..20458
                     /locus_tag="KMT45_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBE7004204.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20451..21524
                     /locus_tag="KMT45_000722"
     CDS             20451..21524
                     /locus_tag="KMT45_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004205.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21672..22190
                     /locus_tag="KMT45_000723"
     CDS             21672..22190
                     /locus_tag="KMT45_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004206.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22171..22563
                     /locus_tag="KMT45_000724"
     CDS             22171..22563
                     /locus_tag="KMT45_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBE7004207.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22639..23574
                     /locus_tag="KMT45_000725"
     CDS             22639..23574
                     /locus_tag="KMT45_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBE7004208.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23707..24486
                     /locus_tag="KMT45_000726"
     CDS             23707..24486
                     /locus_tag="KMT45_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBE7004209.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24479..24796
                     /locus_tag="KMT45_000727"
     CDS             24479..24796
                     /locus_tag="KMT45_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBE7004210.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25040..26650
                     /locus_tag="KMT45_000728"
     CDS             25040..26650
                     /locus_tag="KMT45_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBE7004211.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26807..27655
                     /locus_tag="KMT45_000729"
     CDS             26807..27655
                     /locus_tag="KMT45_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBE7004212.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27886..28365
                     /locus_tag="KMT45_000730"
     CDS             27886..28365
                     /locus_tag="KMT45_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004213.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28398..30626
                     /locus_tag="KMT45_000731"
     CDS             28398..30626
                     /locus_tag="KMT45_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBE7004214.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30755..32398
                     /locus_tag="KMT45_000732"
     CDS             30755..32398
                     /locus_tag="KMT45_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBE7004215.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32471..33016)
                     /locus_tag="KMT45_000733"
     CDS             complement(32471..33016)
                     /locus_tag="KMT45_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBE7004216.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33039..33524)
                     /locus_tag="KMT45_000734"
     CDS             complement(33039..33524)
                     /locus_tag="KMT45_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBE7004217.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33514..34326)
                     /locus_tag="KMT45_000735"
     CDS             complement(33514..34326)
                     /locus_tag="KMT45_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBE7004218.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPILAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34465..>35575)
                     /locus_tag="KMT45_000736"
     CDS             complement(34465..>35575)
                     /locus_tag="KMT45_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBE7004219.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11636 a         6398 c         7117 g        10424 t
ORIGIN      
        1 tacgttaaaa actgttaaaa aacttttact ttagaaaatt ggagaaattg atcgcttaag
       61 ataaattcag gacactaaat agtacaggaa gtagattaaa acttttaaaa gttttaagtc
      121 tatctatttg gcaagtttaa aaaataaaaa ttcggtatca aaaaactgtt accgataaca
      181 tttttgaaag cgttgtcttt tgtttctcta tcacttatag tgatgacgtc atattaaaac
      241 acaaggagga aatcaatgga tacagctgca atttatcatc gaccggaaag tgaatatgca
      301 tttctatata cgaatacaag atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa
      361 aaagtatatg tgctttgtgg agatccatat accataacaa ctgaaaaatg gtatcaaaaa
      421 caacgaccaa tgaaaaaatg tctaagtacc aatgttcatg attattggga aattgaagtg
      481 acgagcgaaa caaggcgctt acagtatgcg ttccatgttg ttggggaaga taacacggac
      541 tgtttttacg gtgatcaagg aatttttcct tatcgagaaa atgtgattac tgaaccaaat
      601 ttttattttc gaatacctta ttttcatcaa atcgatcgat tcactattcc agaatgggta
      661 aaagaaacag tatggtatca aatttttccc gaaagatttg caaacgggga caaagcaaat
      721 gaccccgaac acactttacc ttggggaagt aaagatccag accgagaaga cttcttcggg
      781 ggagatttgc aaggtgtgag ggatcatttg gattacttga cggaccttgg cgtcaatggc
      841 atctattttt gtcccatatt taaagccaca tccaatcata aatacgatac gattgattat
      901 tatgaggtgg atcccgcttt tggcgataaa aaacttttaa aaaacttgat tgatgaagca
      961 cataaaagag ggatacgtat catgttcgat gctgtattca atcatatggg tgtccattca
     1021 ccacagtggc aagacgttct caaaaacgga gaaaaatcga tctataaaga ctggttccac
     1081 atccgttttt tcccagtaga ttcttatcag atgactgact taccagaaac tgctgaaaat
     1141 atcccatatg atacttttgc ttttactcca ttcatgccga aactaaatac agcaaatcca
     1201 gaagtgcaaa aatatttgct ggatatcgca acttattgga taaaagaatt cgatattgat
     1261 ggatggcgtc ttgatgttgc taatgaagtc gatcatcatt tttggaaaaa attcagagaa
     1321 gcagttacag aaatcaaacc agacatctat atccttggag agatttggca ttcctctcaa
     1381 gcttggctgc aaggggatga attccatgca gtgatgaatt atgcattcac agattcgatt
     1441 aaagattatt ttgcaaaaaa gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat
     1501 cagcaaatgc tttacagaga tcaggtgaac gaaggaacat tcaacttatt agattctcat
     1561 gatactgcca ggatcttgac cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg
     1621 gcctttatgt ttcttcaaaa agggtctcct tgtatctatt acgggacaga aatagggatg
     1681 actggtgaag atgacccgga ttgccgcaaa tgtatgatat gggagaagga ggaacaagat
     1741 ctagagctat tcggttttat aaaagaactt gtttctctaa ggaaacagtt gtctaagatt
     1801 atttctgaag gttctactca gtggctaata gtatatgatc gtgaagataa gctctatttc
     1861 acacgggaac tagagggaca gatcatctat gtttacttta atcagtcaaa agaaccttgg
     1921 gtagtagaac aggaaaatga ggtgattctt agtcaaaatt gtcagttgtt agaagacggg
     1981 aaagcagaga tacgacaata cggatttttg atcatgtgtt gaaaactgtt accggtaaca
     2041 acaagaaagc gtttgcaaat gaagatttgt gcccattata ataagggtgt aacagcacgt
     2101 tgaatacaca ggaggaaatg aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt
     2161 taatctcttt aggaatctta ggcggattag cggcttgtgg caatggtaac tcttcagcag
     2221 aaggggaagc cgatgaagga aaaacgttaa cagtttccgt tgattctgga tataaagatt
     2281 atataaacga aataaaaaac actttcgaaa aagaaaacga tgtgaaaatc aagctagtag
     2341 aaaaggacat gttcgatcag ttggaatcat tagcgttaga tgggcctgct ggaaagggac
     2401 ctgacgttat gatggcagca tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg
     2461 cggaagtaaa actgggaaat gaagcagctt acgatgaaac agacaaagcc caagtgactt
     2521 atgatggaaa aatctacgga gaacctgcag taatcgagac ccttgttttg tactataaca
     2581 aagatttagt cgatacagca ccatcaacgt ttaaagattt ggagaatcta tccaaagaca
     2641 gtcgttttgc ttttgagtca gaagcaggaa aaaatacagg atttttagct aaatggactg
     2701 acttttatta ttcctatggt ttaattgcag gttacggtgg ctatgtattt ggtgatgatg
     2761 gcacaaatcc ttcagatatc ggtttgaata atgcaggtgc tgtagaagga atctcttatg
     2821 caacagattg gttcaaaaat gtttggccaa agggaatgca agacataaaa agtgcaggag
     2881 acttcgccag ccagcaattc atgtccaata aaactgcagc aatcattgac ggtccatggc
     2941 aagcgcaaac atacaaagaa aacaatatca attatggtgt agccaaaatt cctactctaa
     3001 ataatggaca gccttatcaa ccgtttggcg gaggaaaagg atgggtagtc agcaattatg
     3061 ctaaaaacaa agacctctct caaaaatggc ttgattatgt aacgaatcag gaaaatcaag
     3121 aaaaattctt tgaaatggta aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa
     3181 aggctaaaaa cgatgaatta acaacagctg tgattgaaca atatgaaaca gctcaagcga
     3241 tgccaaacat cccagaaatg ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg
     3301 cagcatcagg ttcaaaaaca ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg
     3361 aagccataga acaaaaatac tgattaaaat gataaccgtc catttttata aaaaatggaa
     3421 caggtgcttc agataaaagt ggcaagagcg agatggaaaa ttatcttgct cttgttgcca
     3481 ttatcttaaa agcagtttgc ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga
     3541 taaaacaagc aatatcaacg aaaaaaaaat cacgctttgt tcacttattc cagggatggg
     3601 gcagtttgta aatggccaag cttttaaagg tatcgttttt ttcgccatat tcgtcctatt
     3661 cattggtcag atgcttctgg gcggatttca tgcattggta ggattggtta ctttgggaag
     3721 tataccaatg gaagatcatt cattatttat tttgattgaa ggcactttgc aattcatcat
     3781 tacaatcatt ttcttgattt tttacggttt gaatctttta gacgcttatc gagtagcgaa
     3841 gatgcgtcaa gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac ataatatagg
     3901 tgatgaaggt tttccttatc tattgacact tcccgcttat cttctcatga tttttacgat
     3961 cattttccca gtcctagtta cattgttcac tgcatttact aattatgact tcaatcatat
     4021 tccaccagcc agtttaattg attgggtagg ggctaaaaac ttttttagta tcttcttttt
     4081 aagctcatat cgtaatactt tttttgcagt cttttcgtgg accgtgattt ggactttatg
     4141 tgcaacaact ttgcaaatca cccttggtat tctcacagcg gttgttgctc accagaaatt
     4201 tattaaattc aaacgtattt tcggtatcat tttcttatta ccatgggcgg tcccagcttt
     4261 tattactatc atgagttttt cgaatatatt caacgatagc gtaggtgcca tcaatactca
     4321 agtgattcct ttcataaacc agcttccttt tgtagatatc agtgcaatct cttggaaaac
     4381 ggacccgaac tggacgaaag tagcaattat cttagtgcag ggctggttag gatttcccta
     4441 tatctatgtg atggttacag gaatcttaca atcgatttca gaagacttat atgaagcagc
     4501 aaaaatcgac ggagcaaatg catggcagcg cttttcaaac atcacgcttc ctgctgtctt
     4561 cctagtggca gcgccaacat ttattacaca gtatacgggg aatttcaata atttctccat
     4621 gatctatctg tttaacaatg gtggtcccgg aagcgtaggc ggaggagctg gttctactga
     4681 tattcttatt tcctggatct ataaacttac gactggaact tctccgcagt attctatggc
     4741 ttcagcaatc acgttgatta tctccgtcat cgttatctgt gtgtcattgc tggtatttaa
     4801 aaagacaaat gcgttcaata tgggggatta acaatgaaaa aatttcattc gagtgctcga
     4861 ttccaaaaac gactttctca cttttttact tacttttttc ttttaatact gtcagtcatt
     4921 atcatctatc cgcttttgat cacagcaagt actgccttca agtcaggaaa tattgcagct
     4981 tttagtttag atctttcaag tgactggact ttaggaaact ttactcggct attcagtgaa
     5041 acactttacg ggacttggta taaaaataca ttgatcattt ctgtcctaac aatgatcgta
     5101 caagtgactg tagtaacttt ggcgggctat acttatagtc gctatcgttt tatcggccgc
     5161 aaatcaagtt tgatgttctt tttagtgatt cagatggtac cgactatggc agcattgact
     5221 gccttttatg tcatggcttt cttattagaa gcgctcgata agtactggtt cttgacaatg
     5281 atctatatag gcggtggaat tccaatgaat acttggctga tgaaaggata ctttgacact
     5341 gtaccaatag atttagatga atctgctaaa cttgatgggg cagggcattt caggattttt
     5401 ttccagattg tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt
     5461 atgggacctt ttggggattt catgttagct aagttcctgc ttcgaacacc tgaaaatcag
     5521 actgttgctg tcggcttaca aacatttatt tctgatgtga caaaccaaag agtctctctt
     5581 tttgctgctg gggcaatttt gattgcacta ccgatttgtc tattattctt ctttttacaa
     5641 aagaactttg ttaccggcct gacatctggc ggaacaaaag gataggagca gagtatcatg
     5701 caaacatttc cgttaaacta tttttccgca ttacgtacgc caactcaagt gtttgcggga
     5761 cgcaaactat tatcatggcc taaatttttc ttgatatttg tatttttagt ttccttgatg
     5821 gtcatgcctg tgactctttt ttatgccaat cagatccaag cgattccatt agaacaattt
     5881 ctatccgttc attcgctgat cgatgaacaa ggaacccaaa aattttctga acttgaatta
     5941 tctgaaactg ggttacaagc atcgcaacaa acgatagctg tgacccctga aattttggta
     6001 ggtgtgtctt tatctgagaa acaacaatcc gatcatggaa cttttattga ttttgagaag
     6061 gaacaatggg tgatccaaca aaaggataaa tctggtatac gccgctatac gatgaattat
     6121 agcccatctt tccaaccaga ttctgtacgt acgcccgaag atttccaacg atttttagaa
     6181 agagaatttt atgccagtaa tcgtccaaca atcattttat cttatagttt aagtcttggt
     6241 cttgtcctat ttgtcatgac gagtctcatt ttattcgggg cttccttttt cttgtggatg
     6301 acaagaaaaa gccaactttc atccattcat acattcaaag agagtgcaaa tttgatgctg
     6361 aatgttatgg gaataggttc catggcagca gcagttgtag ggtttatcca ctttgacttt
     6421 atcttgatgc taagtgtcca aacaacgata actgtcttgc tgcttttatg ggtttttgtc
     6481 aaaacaagat tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg
     6541 aatgtgaaaa aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc
     6601 ttagttgatt cttttctttt ttagtattaa cgtaataggg tagttttttt aaggaggaaa
     6661 atcatgttag agaaaacttt atacaatcaa ttattttcac gttctttctc gctccctgtc
     6721 gaagtaaatt attgggacgg tacaacgaaa caatacggag atacggacac tcctccccaa
     6781 atcaaaatca atatccatga agaaatccca atgaaagaga ttacaagtaa cgcttctttg
     6841 gcactaggag aagcctatat ggatcatcga atcgagatcg agggaagtat ccaagcattg
     6901 atcaacgatg cttatcagca agcaaacagc tttttacgag gaaaagatta tctaaaatgg
     6961 ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca tgcgcattat
     7021 gacttaggta acgactttta taaactttgg ctagatccaa ctttgactta ttcttgtgct
     7081 tatttcaata cacctgaaga tacgttggaa caagcacaaa tcaataaagt ccatcatatc
     7141 ttagacaaac tctttatcaa agaaggcgaa acgttgcttg atatcggttg tggctgggga
     7201 acattgatgt tcactgctac caaagagtat aatgtcaagg caactgggat cacactaagt
     7261 gaagaacaat acgatttgat taccaaaaga ataaaagaag aacatttaga agataaatgc
     7321 cgtgtattat taatggatta ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt
     7381 gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag gttatttcaa agtggtaaaa
     7441 gatttattga aaccaaaagg aacagctttg atccatggaa tcagccgaca acaaggcggt
     7501 gcgacaaatg cttggatcaa taaatatatt ttccctggcg gttatgtccc tggtgttact
     7561 gaattagttg ataatattac aaaaaacgat ttgcaactga ttgatttgga aagcttacgt
     7621 cgtgactacc aattgacatt agaacattgg acaaagaatt ttcatcaagt ccaagatcaa
     7681 gtcacgaaag aaatgggtga ttccttctat cgaatgtggg atctatattt acaagcttgt
     7741 gcagcttcat ttgaatcaag taatatcgat gtgattcaat atttgcttgt tcaacctgga
     7801 aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt aaagactatt
     7861 taaaaagaag ctaagtttta tgatgctcca gaagtgaact tttttgtcta acttctagga
     7921 ttgtctctaa agcttagttt ctttttcttt atattttcat aataaaagtt tggttcttta
     7981 atttttaaac atgacagaaa attttttctt cgttatactt gtaatagaat actattttat
     8041 aacaaagggg atcgaatcaa tgaaaaaaac tgttatttat actagtttat ttgcagcttt
     8101 gtttttagct gcttgtcaat caaataccaa tcaaggcaca tctgggaata cgtcttccag
     8161 tagcgaacag cgctcttcac aggtatcgga ctcctcaaca aaagcaagca catcttcgtc
     8221 tagccagaca aaaagttcta gtgaagcaag tactcagtca gatatcaaag tgtctgcagc
     8281 tcgtgcgatc gaattgttcc aagataaata cccagatact gcaattacta gtttggaatt
     8341 agatagtgat tggggatctt acttctacaa gatagaaggt gtcgatgatc aaaaagaata
     8401 cgaagcaaga attgatgctc aaaatgaaaa aatggagaca gagaatccag aaactctaga
     8461 tcgagatgaa caaaatggtc aaaagaaaaa agaagatggt ctggatgttg ataaattgat
     8521 cacgattgag gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta cggcaacaga
     8581 ttgggatttg gataaagatt taggtactac ttattgggaa gtcaaagtaa aagacggcaa
     8641 tcaaagtaca tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa ctgaacaaga
     8701 tgattaagaa tttcttcgga aaacaagagt tgatttttct tttagtcatg atacactaga
     8761 caaaataact tgtttgaagg agcgatttac ttgagtttag aaacgacctt tgccaatcat
     8821 atctttgcca atcctttgat gaatgcatct ggtgtccact gcatgactac ccaggaatta
     8881 gatgaactgg ctcattcaga agcaggagca tttatcacta agagctgtac gatcaatgaa
     8941 cgaaaaggaa atcccgaacc acgctatttt gatgttcctt tagggagtat caattctatg
     9001 ggacttccaa acttaggttt ttcttattat ctagagtatg ctcttgcgta tgaaaaagtg
     9061 caggaaaacc aaaatcaacc gttatttttt tccattgccg ggatgagtgt ccaggaaaac
     9121 ttggaaatgc ttgagaaaat agaaaaaagt gggtttaatg ggatcactga attaaattta
     9181 tcttgtccca atgtgcctgg aaaaccacaa ctagcttacg attttgaggc cacttatgaa
     9241 acactgaaag aagtattcag catcttttct aagccgctag gaatcaaatt gcctccttat
     9301 tttgattttg ctcattttga tcagatggct gatatcttga atcagtttcc attgacctat
     9361 gtcaatgcta tcaacagtgt gggcaatggt ttgtatatcg atacagaaca agaagcagta
     9421 gtcatcaaac caaaagaagg gttcggcggc attggcggag agtatatcaa gccgactgct
     9481 ttggcgaatg ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat cattggtaca
     9541 ggtgggattc ggacaggaca agatgcattt gaacatctgc tatgtggcgc aagcatgtta
     9601 cagattggta ccgagcttca taaagaaggg ccggaaattt tttctagaat cataaaagaa
     9661 ttaacgcaga ttatgtcaga aaaaggatat acgtcaattg acgaatttaa aggcaaatta
     9721 cgcaccattt catagcaaag agacgaaggt catgattgat cttcgtcttt ttttataaaa
     9781 aacactcgtc tgttcattcc tgtgcaagcg tactcattgt atgttagacg aacttgtggg
     9841 aaaataacag aggcaaaaag gaggataata tgaatgatgc aacagaatta gcaaaagagc
     9901 ttaaagaaaa agaatttagt tttcaagaac tagttacgga tatacagaaa aaaataaaga
     9961 agcaaaatca tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt aattcccacg
    10021 agctattgat tccccagtca gaaaaagggc cattggctgg tatcccattt cctttaaaaa
    10081 tgctcggtca agaaaaaaag ggatggctag ctacttcagg atctcgttta tttgaaacac
    10141 accgagcttc tcatacatct aattatgtgc ggcaagcaga agcgataggt ctagttcctt
    10201 ttggacaaac caatgcgcca gagtttggtt tcaaaaatat tacagaccca gtcatctatg
    10261 gcccagcaag aaacccttgg aaccttgaat gtacaccggg cggatcaagt ggaggagctg
    10321 ctgctgcagt ttcatcagga atcgttcctt tagctggtgc tagtgatggc ggtggttcta
    10381 ttagaatacc cgcttcgttt tgcggattga ttgggctgaa accttcgcga gggacgatgc
    10441 cagttggtcc atatgcttgg agaggatggc aaggagctgc tattgatttc ggcttgacag
    10501 tttctatgag agacacggag gctttatttt atgggatgag aagtatccat tccggtgcgc
    10561 catatcaagt atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg ttgaagatag
    10621 ctttttgtgt tgattcaccg atccactcac agattagtga agaggcagta cttgctgtaa
    10681 aaaaagctgt agatttcctt ggaaaagaag ggcatgattt gacagagatt ccttacccac
    10741 tagatggtcg taaactgatc acgtcttatt atcagatgaa tggcgcagaa acagcagcaa
    10801 tgatccgctc gattgaaaca ggcatccagc gagctgtcag aaaagaagaa cttgaaccca
    10861 tttcttggac tttgttccaa tatggtcaga agctctctgc cgctacttac atcgattctc
    10921 tgcatgtatg ggatgaagca gcaattgtca tggaaaacct acatcaagaa tacgatctat
    10981 tcctttcacc aacgactgca tttaccgccc ctaaaatcag cgaagatttg caaagtgatg
    11041 cgataagagc acagatggaa cacatcagtg attgttctga agtagaaggt ttagacgttg
    11101 tttcttccat gtttgaggag agcttgaaga tcacacctta tacacagttg gctaatctga
    11161 ctggacaacc agcaatcagc ttgcctactc atttgacaaa agaaaacatg cctctaggaa
    11221 tccaatttat ggctgccaga ggaagagaag atttgctatt tcaaatcggc tatctatttg
    11281 aagcaaatca tcagttccat ttaccaaatc catggaaata gcagaaacaa ggagaagaag
    11341 aatgaagaaa aaaagattgt ttgctctaat aactagtgcc ttttttattt taagcgcttg
    11401 cggtacagct gccgctaatc aggaagataa cagctggagt aatgtagaaa aaaagaaaga
    11461 attagtagta gcgacttctg gcacactgtt tcccgcatct tattataatg atcaaaatga
    11521 attagttggt tatgatgtag atttggcaaa agaggtggct aaacggttaa atgtcaaaat
    11581 ctcctttaaa gaatacaacg tggatggaca aatcacttct gtttcaaaaa gagaatcaga
    11641 ttttgctgcg aacgattttg gtatcacaaa agaaagagaa aaaaaattct ccctttcaga
    11701 gactttcaaa tattcatttg acagtatgat cgtacgaaaa aaagatgatt caggaatcca
    11761 ctcacttgaa gatcttaaag ggaaaaaagc agctggcgaa cccaatacta gctatatgcg
    11821 tttagcggaa aaacacggag ctaaattagt cacatatgac aatgccacga atgatcagta
    11881 tttaagcgat gtagcaaatg gtcgaacaga tgttattcta aatgattatt atctgcagaa
    11941 aatggccgta tctgctttac cagatatccc tgtaaaaata ctggagaacg tttatttcaa
    12001 tgaaaacgaa agtggttttt tattgaaaga aggaaatgat gctctgaaaa agaaaatcga
    12061 tggcgtatta aaagagatga aagaagatgg gacgacaaag aaaatatctg aaaaatattt
    12121 tcaaactgat gtttcagtca aaccgaaaga aacgatggct gagtcggtga gtgagtgatg
    12181 tactttcctc taattgattt ttccctcatg tggacttcac ttcctttcgt actgcaaggc
    12241 ttggtataca cattagggat tggcttcgtg tcgtttcttt taggaaatct ggtagggttg
    12301 cttttgacag ttttaggtct tttggattgg ctgcctctga atgtatttat tcgtttttat
    12361 ttatcgttct ttagaggcat tcccgcacta gtcctgctgt ttctgttata ttttgggtta
    12421 ccttatcaat taagtgcctt aacggcttca gtcatctgct ttacgattac cagcagtgca
    12481 tttattggtg aaatctatcg aggatcacta gcaggcgttt cttcaggtca gtgggacgct
    12541 ggtttagctt ctggtttttc tttctttggg gtattgaaat atatcgtttt gccgcaagct
    12601 tttcgaatct cagtaccggc actcagtaac gtagctatgg atctagtgaa aggtacttcg
    12661 ttagcagcaa caatcacagt gccagaaata tttcaaaaag caaagatcat aggcggtcgg
    12721 acattcgatt atttttctct ctatatttta gttgcactga tttattgggt catttgtatc
    12781 gggatcgaaa aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc aatctgaaaa
    12841 atagaaatgg tgctagcgtg gctggcacca tttctatttt ttgtttcttg tatattctgc
    12901 ctagtatttt aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg gaggtagaaa
    12961 acatgaagac cccgctaacc atcgaaatag aatcctcgtt ataccaatat tgcatcgagc
    13021 aaggcggctt ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt gtagatacgc
    13081 ttagctgctt gacaaaattt gatggaagcc gagaatggcg atgctatgag ctgaaagtga
    13141 caaaagcaga ttttcgctct acagccaaac tgtcatttgt tggacattat aattattttg
    13201 ttttaccaag taaactatat gaagaagtaa aaactgaaat ccctacagga ataggtgttt
    13261 tagtgtatcg tccgtacacg gttgaagagg agatgcctgc ctctggtaca tttctgattg
    13321 cgaaaaaagc agcaaagaaa gagcttgctg taccggaaga agctttgaca aaccgcttca
    13381 tggcttcttt attcagggaa gtaagaaaag caaagcaaat ggagcgaggc gtaaaatatt
    13441 ttccaacaga tcaattgtac aaagaattga aaagacgttc agaaaatcaa gatccatttt
    13501 ctaacgaacg atattatgaa cggtttttag aggaaatagc tagtcaggca atcaatgagt
    13561 tgcaagaaga agttatcgcg cttcagcatg attatgaata tttgaggcaa caacagcaga
    13621 tccgtcggtt gcctacagag ccattggagt gaaaaatatg tactatcctt atttaagagg
    13681 aaaacaattt gacctattag cattaaaaga agcactgtcg cgaggattgc tctccaataa
    13741 aatccaacca gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg tgatcgaatt
    13801 atttcaaaaa aagcaccatc gaatcgctgt gatccaaaat ccccaagtag gacaattcaa
    13861 attattcgat cagcatgtgc acagttggac agtcaaagaa aatagctctg tcgtccctgc
    13921 acagatctta acgccagaaa atctcagtga agtactggat tcacctccag cgtttcttgt
    13981 tttcgatgga cagcactacc caaaagatac agaggtgtgg aaacagttgg caggagttga
    14041 ttcgaaattt ttgataccag atactagtcg ctttcgcatc tggctgcccg aaaacaaaat
    14101 tgtgatccgt gactcttttc aaacgagaaa gcatgttgaa agttatgcag ataaaacaga
    14161 tgactttttt agtgatgact atcttttttt ccacgcagac ggatatattg gattttctga
    14221 tttcacgatc gaaggtagcc gttatttcga taaagggttc cctagccgag cgcttgctat
    14281 ccatctaacg tatattgatg cttacggcaa tatacgcgtg aaacattttg tctctgacag
    14341 taacgactct gctaaagacc aagcattgaa gtttcttgaa gcaggagata aaatgaaaaa
    14401 atggatcatg cgtcaccatc atcaactctt gattacttca ggaatgattg aattattggc
    14461 tctttaccag caacaaaaat tccctggact gggagtgttg aaaaagtggt cacttatgca
    14521 tcatttggaa cttattagtc acctgctaga tcatccaaaa gattggctta aatattattc
    14581 aaaagttcag gaactatatg aagtgatcca atgattagat aatgtaaatg aaaaaggaat
    14641 agaaaagagg aaactggatg tatattcggc aaacaaggaa agaagaagta gaagaagtgg
    14701 tagaaatcat taatgccgca gcggatttca ttgcttcgca aggctctcct caatggcaaa
    14761 acgggcagaa accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa gaaagctatg
    14821 tattaatagt ggaagatcaa attgctggaa cagctgcatt agtctcaagc gtggatcccg
    14881 tttacacaaa aatagatggt agttggaaag aatcaactgg tccttattta tcgattcatc
    14941 gtgtcgcttt agcagaaagt actcgaggaa aaaaactagc aaaaccgttc ttagaacatt
    15001 tgttgacgat tgcagccagc aaaaatatcc aagatatccg tatcgatact tatccagcta
    15061 atgaaccgat ggaaaagacc atctattctg ctggattcac ttatcaaggg atgattgaat
    15121 ttccttttga acacggcgaa agaaaagcat atcaaataat catcaattga ctataaaatc
    15181 atctgagcaa agataaagac attaacacta ctgaaaaaaa caatcatatg atagccaaga
    15241 ggttgtaaca gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa atgcttctca
    15301 aatttttgtg gaattttagc gtattttacg aaatagctgc tttctgaaac tcccttcatc
    15361 gagaggtcgt gacatagctt ttgtcacgac ctctcggctt atcctacgag tattattcga
    15421 tacatttata tcaataatcc tttttttatt ttatctgcag cttcttggaa cgctccatcg
    15481 tttgacacga ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc taaatcttct
    15541 acataaaccg ccagtctggc tgaaatctgt tcagatgaat ctccacgttt tttcaggcgc
    15601 gattgcaatg tttctttaga gatcgtaata aagataggac ggactaccgt cccgaaatac
    15661 tcatataaat gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc tactgtcagt
    15721 ttctttttaa tttctgcttt actggaacca tatttatgtt gagcatattg gtcccactct
    15781 gcaaactcat ttcccttgat catctgattg aatgtattgt cagaaacaaa ataataatcc
    15841 tcttgatctt tttccccagg tcttggtgat cgattcgtgt aagtaatgat cttttgttcc
    15901 ggctgaaaaa tagcttctgc gagtgaagtt tttccagaac cagaaggacc gataaataca
    15961 tagcaataat tcattgtcat ttctcctttg atgattgaac aagtgaaaaa gctttggaaa
    16021 atatttccaa agcttttaaa agtaactttg cttttttgta tattatacca gcaagccagt
    16081 cacaatagca cttaaaaagc tgactaaagt cgctgttagc aaaattttca atccgtgttt
    16141 tgcgacaaga tttccttttt cttcatttaa tcccttcatc gcaccagaaa tgatcccgat
    16201 agaagagaag ttggcaaatg aaacgaggaa gacagatata attgctgttg tacgctcagt
    16261 aaaatggaaa ttaccatttg ctaattctgt catagcaaca aattcatttg taaccagttt
    16321 agtcgccatg atactaccgg catccactgc ttctttccat gggattccgc tgatgaaggc
    16381 aagaggggca aagatgtaac ctaggatctc ttggaaagtg atgccgaata tcccgtggaa
    16441 gatagcattg atcatggcaa tcaaagcaac aaaaccgata agcatagcag caaccgtgat
    16501 cgccacatgg aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg tctgttcttt
    16561 gttttcttca atgatcagct catcttcttt ttcttctaat acatagggat ttacaattga
    16621 agccagaata taaccgccaa ataagttcaa aacaagggca gtgatcacat atttagaatc
    16681 gatcagtgcc atataagatc cgacgatcga catagaaact gtagacatag ccgatacact
    16741 catggtgaac aaacgtttct cagataggaa gggtaattcc ttttttatgg aaataaaaac
    16801 ttccgattgt cctaaaatag cagaagctac tccattataa gattccaatt tgcccatccc
    16861 gttgatttta cttaatccta gaccaacagc tttcataaat aatggaagaa tcttgatata
    16921 gcgtagaata ccgataatag cagaaataaa tacaattggc atcaatacgg aaagaaagaa
    16981 aggtgcactt cctttatttg ctacaccggc aaaaacaaag tcaacacctt gcccagcaaa
    17041 agcaagaaga tgatcaaaca tttttgccaa tacagaaaca accgctgtac caaaagttgt
    17101 acgtaataat acgaaaccaa gaacaagctg taaaacgaac atcacaatta gtggtttcaa
    17161 tttgattgct tttttgttac tgctgatcaa aaaagcaagc cctaatgtga caaataaacc
    17221 aataatacct aataaataac tcaaataatc actcctattc caaattccgt tttattttac
    17281 ctgttttgca aataaaatac taatctttag tgaaattttc attacttttt tagagtgaaa
    17341 cgcattcatt ttccattaga aaataaagcg aacaaaataa aaggtttaaa aagtgtaacg
    17401 tcttagtaaa gtctataaaa aaacgaaact atcgattaag ctaatagttt cgttttcaac
    17461 atttataagt aaactttttc tattggtaat atagattttc ggaagggtag acaggtgcgc
    17521 aggtaatatc gattcctagt tttttcaatg tttgttcatc atttttattg accatcacag
    17581 tagagtgggc ttgtgctccc tcaagttcag ctagtttatc ataagcaact tgagcgattg
    17641 gattcgttac tgcactgatt gataaagcga tcaagacttc attgatgctt agtgcatcca
    17701 aagaggaatg aagtgtcttt gatttcaatc cttgtattgt ttcaagaact aatggtgaca
    17761 ataaaggaat gtcatctgcg atatgggcta aatgttttac tgcatttaag ataactgccg
    17821 ctgacgcatc catcaatgaa cttgtacgcc cggtaatgat gacttcatct ggtaattcga
    17881 ttgcaatcac agcagaagga tcttttgtag gactttgcgc ttgaatctta tctgcatagt
    17941 catgggcagg gactaccacg tttcggtctt caggacgtaa accgacttct tccataatca
    18001 gtttgattcg attaactgct tcttcgtcga tcaatccttt tttgaagtcg cattctgttt
    18061 ggaaataacg acggatgatt tcttgtttag aggcttcttg gacaacggta tcatccacga
    18121 tcccaaatcc tacacgattc acacccatgt cagtaggaga ttgataaaca gattctttac
    18181 cagtaatttt ttcaataata cgtttgatta caggaaatgt ctcaacatcc cgattatagt
    18241 tcacagctac tttattgtag gcatcaaagt ggaaggaatc gatcatattg acatctttta
    18301 gatcgactgt ggctgcttca taagcaatgt tcagcggatg tttcaacgga acattccata
    18361 ctgggaaagt ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt tcatgataca
    18421 gttggttcaa ggaagtagcg agctttccac ttcctgggcc aggagctgtc actacaacga
    18481 ttggtttagt tgtttcaata tactcatttt ttccaaaacc atcttctgag acgatttttt
    18541 ctacgttgac tggatagtct tcgatttctg agtgtttata gaccttgatg tttcgtcttt
    18601 ctagtttatt gataaagact ttcgtggcag gctgtccact atagcgagtg ataacgacac
    18661 tgttgattaa taagccataa ccacgtaatt catcgattaa ccgcaagata tccatatcat
    18721 aagtaatccc ataatcacca cggatcttat tacgttcaat atctccagca tagacgcaaa
    18781 tcagtatttc agcttgatct ttcaattttt gcagcaattt gattttcgca tcttcttcaa
    18841 atcctggaag tacacgtttc gcatgcttgt cgtcaactag cttcccacca aattctaaat
    18901 ataatttgtc atagtggttc actcgttcca aaatatacgc tgactgctct tcaatatatt
    18961 tttctgaatc aaaaccaatt tttttcacta ggttccctcc ttaagtacta aagcattttt
    19021 tcattttttt gaggattcgt caactggaag agcgaaattc ctcaaaaaaa caaagaaaat
    19081 ggtttaaacc aacttgtcta gatagttatt cattggtatg atagtttttg ataaggaagg
    19141 tgctacatga aaaaaagaca gaaaaagaaa aacgcatata aacactatat ccgttcgatt
    19201 tttaccggtt atgaaagaat gcttgaagat cccgaattgg aacaattgac gtttacttat
    19261 ctaaacgaag aaacgcagct aactcgtgat gagcatcagc gcattcattt cacaacaaga
    19321 gatctgccta gtaaataaaa aataagataa aggtcatcgg attcgattct gtcctatcag
    19381 atggaaagac tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag cctgttttat
    19441 ggagcattgc ccgctctttt ttataacaat tttttacaga aaatggtact attgaagtat
    19501 taataagtga aaaacagatg agtaagagga ggcaagtagt atgaaagagc cagatacaaa
    19561 tgcaaattat gagccagacg aactagagga gacgaatgcg gatcaggata caggacaaga
    19621 atcgtcttct ttttacgata tcaaagaaaa agcaagcatg aatctttccc aattattaga
    19681 agacttacag gcttttgaaa aagctgtccg cgaagaagat atccctgaga tctaccgttt
    19741 atacaatggc cgattgaatg aagaattaaa aagaagctca aatgaaaatc acgagatcga
    19801 tcaattgctg atgcgtaaga tccatgatcg ttttttacag gaatttcctt ttatggaaca
    19861 agtagaaaac atctcgccaa cgatgagcta ttacaaaatc ggcacttatt accatgatcg
    19921 gcccacggtc ggtattgatg caagtctgcc agaaattttt gttttgccta agatcgatga
    19981 agagtgggag aaatattccc aaccggagtc aacggactat gacaaaaaaa tcaatgagct
    20041 agacgcaaag gtgatcactg ctcagacaga aattgaacgt ttagatgaac aaataaaaga
    20101 aatcaatcga caaatgactg atttgaacga caataaagga ttcttgaatc gtaaaaaaat
    20161 cgaggaagaa attcaagaat tagagaagaa gaagcaagta ctatctaatg aaaaattagg
    20221 ctggcttcct tatattgaga cgcctgaaac aatccagcag caaaaagaag cattgaagca
    20281 agaagcaaga gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag
    20341 acgctatttc ggtagcaaag agggattcgg tcaagcaatc catgaattct tgatgaatta
    20401 tcttggcaat gagaatccta ataatcaatc taatgaagga ggttccgagt atgaatgaac
    20461 acgatgaagc agaatggaca aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca
    20521 cttcattgga agaacaaaca agtaatcctc tggatgacac agaagaaatc cctatgctag
    20581 aggaaacaaa aatacacgaa aaagaaaaag taactccgac gaatacgaat actgcagaaa
    20641 acacccatgc ttacgaaata actgaaaatc ccgggtattt agaaggaaaa gagcaagatc
    20701 atcaagatat cgtcagtttg attgaaacag cggaagcaga attagcggat ttacgtatga
    20761 gaaaagattt gatcgaatca gattttccac aattattaga atcatatgat cgttttatat
    20821 tgaatgaaga accaatctcc acgatcggaa agacaagcct gatcgaatac ttcacgtatg
    20881 aattactgaa accgttgaat gatattggtc tggaacggtc aaattcagct tatgacacat
    20941 ggaaaacaag acatttttcg atcaattata cgttgaatgg acaaaacgaa ttagtctttt
    21001 cctttgtatt accgaccatc gatcaacgat accaagtgga atcgatgagt ctcctaaacg
    21061 tatcaccaga gacaatggaa atcaatgtcc aagatgaccg ggtgctttct ttgatccgac
    21121 actggtcagt ggatcgagta ttctctgcag ggcagatcac gatattcaat cacaaattga
    21181 atcaattatt agcccatgca agaacacttg gttttaccgt taatcagaca ttactagaca
    21241 atacgaagcc acttcgtttg aatcttcaga gcgaatttga attgacgaat gaagtattgg
    21301 atgatatttt tatcgtggca atgaaaaatc catcctacga tatggaaaag atcaacgaaa
    21361 ctacttatca agtacgatta gataaaggac agtctttaac gataacaaca aacgaaaaag
    21421 atcaaacagt cttgtcgatc tcatctggag actacccacg atctgtcatt gatttcttta
    21481 tcaattacga attcttggtc ccattaatgg tgagaaagtc ctgattggat aagttggatt
    21541 accagatgtc attcagataa ttctgttcca aaggacttgc ggcagttatc cagaataatt
    21601 gtcacaagtc ttttctgtat gtactaaatg gacgtgaata acttgctgat gaaggaggga
    21661 tttcattagg aatgtccaca aataaaatag cacagcttag cttattgtct gctgcttgtg
    21721 tagcaggtag gatcgctttt caatttattc cgaatttcca gccaatgact gctatttttt
    21781 tatttatagt actttactta agcttaaaag atgcgttaat tgtcatgagt ctttctattg
    21841 cgatcagcag tttttatttt ggtgttggac catgggtcat cggccaatgg atcagctata
    21901 ctgttgttct gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat
    21961 ggttaaaggg agtctgtttt tttctggcgg gcatggttta cggaataggt atgaccgttt
    22021 ttgatacgct gttatatcgt ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt
    22081 ttgatttgat gcacagtata ggcaatgttg tgttctttct tgtttttctg ccagtcgtca
    22141 aacgttttta caatcattca ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca
    22201 atgagtgtca gtatactcat tttgacaagt tgcagtacaa cctatacaca atcggcttcc
    22261 aaccaaaagg aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt
    22321 caaaactttt cggaaaaaga agtgttcttc aaaaaaggcg atgatttact gagtattttg
    22381 aaacgaaatt ttgaagtcaa agaagataac ggcttcatca cttcgcttga agggcactca
    22441 caggatgaga aaaatcaact ctattggatg tttactgtgg acgggaaaag tgctactact
    22501 ggtgccaaag agatcaaatt gaatgatgga caatcggtca ttttcaattt gtctaaacta
    22561 taagtttcat aaaaaactgt taaagggcga atattgccat aattcagtga gttcagttga
    22621 tgaaagaagg aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct
    22681 gctctagggg ctaatattct agttgctatc agcaaatttg tcggctatgc aatcagtggc
    22741 agtgccgcca tgctaaacga aagcatccac agtatcgttg attgcagcaa tcagattttg
    22801 ctgttattcg gtgataaaca agccaataaa ggacagagcg agttgcatca atttggtgaa
    22861 ggacgagcaa aatacttttt cagtacaatc gtcgcaatga tgcttttctt cggcggtggg
    22921 gcacttgggg tgatggaagc ttttgaaaag ttgacgcatc catcccatga agtgggtaat
    22981 ccgatcattg ttatagcaat actgttattt ggtttggtaa tcgaaggaag ttcgttacgc
    23041 gtggcaatga aagaaatcaa agaattgaat actgaaaaac tttccacgat gcgctttttg
    23101 cgcgaaagtc gacacagtga aatactgatt atttttacgg aagacttgtg tgctgtaatc
    23161 ggtttggtct tggctttagc aggaacaata ttgacttatg caaccgggat tgctgcattt
    23221 gatgcgatca gcggattgct gatcggtttc ttgctgatgg gagccgccat tttcctagca
    23281 aaagaatttt atagtctgat catcggtgaa agcgtgacag caagcgattt atcaaaaatc
    23341 aagttagctt ttgaacgtcc tgagatcagt cggctgatcg atataaaaac agttcattta
    23401 agtccaacgg agattttagt tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa
    23461 gcttactcgg tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag
    23521 atgtatatat acattgaaac agataaatat gacccaaatt attctcgtaa ataaaacgtt
    23581 tattcatgaa ttgaagcaaa aatgagaaaa atataatctt gtttttaaat aaaacggcta
    23641 caatttagcg aatttatgtt acaatgagtc cgctgtttat ttgttgtaat taaaggagat
    23701 attttaatga acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc agtgatgcca
    23761 ctttgtataa gctatatacc agtaggatta gcttgcggag tattgttgca aaaagttgga
    23821 tttgatcctc tcttatcagg actgttatcg attctggtct tttccggtgg cgcgcagttt
    23881 ttagttgcat ctatgcttac gacgcaagca tcatttgcaa cgacattatt gatggttttc
    23941 tttttagaat tacgctatac attattagga gcaagtcttg ccggttttat gaaaaaagaa
    24001 aagcggacgt ttttggctgt cttttctcaa tcactgaatg atgaaaacta tgcggtgaac
    24061 tacttaaagt tttcaactga cccttcctgg aataaaagaa aggctttata tgtcaattgg
    24121 ttctcaatga ctgcatgggc cggtagcaat atgatcggta attttttcgg ctcggtgatt
    24181 cgagtagatg ctgaccttgt ccattttgct ttgacggcca tgttcatttt catgtttatc
    24241 atgcagatga aaagcgcact actgatcttt acaggtttat tctcaggcgt attaggggtc
    24301 gtattcatgg tattattcca aaatactttc ggattgattg cagcaacagt cattgcttca
    24361 tttgccggat ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa actaagagac
    24421 cgcggtaaag atgtcaatga gccgctcttc aacttcccgg aacaggaggc caatcaccat
    24481 gactaaacta tatttgctac ttttagttgc ttgtctgttc atcgtggcat atatcccgcg
    24541 cttgttcccg atgctttatt ttactcatcg aaaagtgcca tcctggttca gtgactggat
    24601 gaaatatgta ccggtggcat tatttgcggc gttagctttt aaagatgttt tcattactca
    24661 tgaacatctg gatattgctt ggaatatcaa aatagctgca atgattctag tagcaggcgt
    24721 ggcttacaaa acacgttcga tggcgttgtc agttttgaca ggacttgcgt ctgtcttttt
    24781 gctgtctatg ctttaaactg ttttatttta ttaaaaacag agattgcgac ataagttgtt
    24841 caaactgatc tatccgaact gcatttgcaa cagtcaattc tggatgcaag tttattcgta
    24901 gggataaaaa agattagtgt tggcttatga tgcaagctct tttttatttt aggaattaga
    24961 gagggatttg tccttctatg attttttgct ataataggga agatgtttat tttatgaaag
    25021 aatagttgag ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt ggctaacctt
    25081 tgctaatctt ctatgtaaag ttttaggggt agtctatttg attccctggc taagtatgat
    25141 ggggaacaat caagatggta tgcttgctac gacattgtat aatgttggct atcttcctta
    25201 tggattgttc ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa aaaaagtagc
    25261 tgtagccaca aaagaaggag ataacgctgc ttgtcggctg atttttagaa gtacgatcaa
    25321 tatcatgttt gtcatcggta tcgcctctgc tgcactgatg tatttatttg ctcctttact
    25381 agccggaatc agtccgattg ctaacgttaa taatgggata ctagctatca gaagtttatg
    25441 cccatctttg attgctatcc caattcttag tgcaatgaga ggatatttcc aaggaaaaaa
    25501 tgatctgcgg ccttatggaa cttctttgat tatcgaacag gctgtgcgag tgatcgttat
    25561 cttggctggt acttattact tgcgcgtatt gactgacgga accatattag aagctgtatt
    25621 gatcagtaca gtcgcttctt tcttcggtgg gttagctgcg attatccata tgtatatcgt
    25681 cggacgcaga aaagacttct tcgaacttaa agattttttg atttccagcc gttatttcga
    25741 aagagaaaac cgatctgcct ctgtctctat cattcgggaa acactaccgt ttatttttgt
    25801 cggctcagtc attactatcg ttcaaatgat cgaccaagta acgatgaaac cactgcttca
    25861 ctttttccgt ccagagatcg cagatcagca actggaattc ttattcagcc gtgcttctgt
    25921 caatccaaat aaattgacat tgatcctgat ctccatggta ggaacagtag ctataagcag
    25981 cttgccaata ttaagcacga tgaagaaaaa agatcggata caaatcgaaa aaacagtagg
    26041 agatagtttt tcgattgcgt tattgattct tcttccttca ttgactggaa tgtctttatt
    26101 agctggacca ctatacacat tgttttttgg ctatgatcct gaaagtgtcg gatatttcca
    26161 aatggctttg ctagcttctt tgtttttctc tttattcacg atcctatcta cgatgcttca
    26221 atctttgaat caccatcgtt ttgcgatccg attaactgta gaagcaatta tcctgaaagt
    26281 catcttccaa gtaatcggat taggattagt tggcggatac gggatgagct tatcaaatgg
    26341 cttagcattc ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag aataccgaat
    26401 agcaccttta gcaaagatca gcaatttttt cttaaaaact ttccgctcca ccctgattat
    26461 gcttgcagta tgttttgccg ttttcttttt gctgaatcag cgattgtcaa tggaatcaaa
    26521 aagctatgca atgatttatt gtgtggctgt aggaagtatt ggcggcttag tatttttatt
    26581 ttctcaattc ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc atagcagaag
    26641 caaagaatag gttaactaat cgttttttgc tgtcacttga aaaacggcaa ctcgtatgat
    26701 tacgcttgtc gtttttctat tttccaattt tagtttcata tctaatctta cttatgatat
    26761 agttataagg tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga aacttgcagt
    26821 agttacagat agttcggcat atctgccgga acgtatcaag aaccatcccg acttattcgt
    26881 tattccaatc cctttgatta tggatgggaa aatttataac gaaggaatcg atatcgaagc
    26941 ggatgaatat tatggacttt taaaaaacag taaagatttt cctactactt cacaacctgt
    27001 cgtaggggaa gttttgggtt tatatgagga attgaaagag aaagggtatg acacgatttt
    27061 aagtatccat ctttcttcag ggatttctgg ctttgtcaac acattatttg cgatgaaaga
    27121 tgacatcagc ggagtaacgg tcattcctta tgactctaag atcactagta tgccgatggg
    27181 acacatggta gaagctgcct tagacttaaa tgatgaaggt cagtcccttg atgaaatact
    27241 tgcacatatc gatcgaatcc gagataatac ctacgcttat ctgattgtag atgatttgaa
    27301 caatttagta cgaggaggac gtttgacaaa tggtgcagcg ctgattggca gcttattaaa
    27361 aatcaaaccg atcctgacat tttctgaagg gaaaattgtt ttattcgaaa aaatccgttc
    27421 aagtaaaaaa gcatttgctc gagctgaaga aattattggt agaagaaata acgaaataca
    27481 accgccagtc aaactttacg tgatacatgc aaataattta gaagtagcga ttgcggagaa
    27541 agagaaattg cagaaaaaat atccaaatgc agaaatcgaa attggtcatt tcggaccagt
    27601 cattggtaca catttaggtg aaaaagccat cggcattgcc atttcagcgc aataattcct
    27661 tttattcaaa aaacatacca aaaatattcc aacatcatct tggactttct acatgttact
    27721 aatgagcgtg taaaagaacg agatggtgtt tttatttcta tcaatgaagc tctttttctt
    27781 tgcaatcctc acttcaatcc tctataatgt cgcatatagt ttagtaaatg agtaaactac
    27841 taatttactt atttactaaa aataaaagga tgaggagtta aaataatgga aataccaaca
    27901 aatttaaaac ataaaccagt aatcgttgta gaagactatg ataaagttga tggaagaaat
    27961 gcattaaata cggatgcaaa agggctttca ctgggccttg ctcagtggaa tgatcgtgga
    28021 aaagtcgata tctcaggcaa ggtatggcga catactggag aaaaatggtc acgacagtcg
    28081 gaagaattac caatcacacg tattttagat ttagcgatct tagctgcaca aggaagttta
    28141 tacttccaag atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc agtcgtagac
    28201 atcattggat tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa tccaaagatc
    28261 gatgaagaca tcattttgta ttacgataca ctacaaaaag acggagaact gattggggaa
    28321 cgattccgta ttttaaaacg tcttttagat gagctaggat attaatagac agagatgatc
    28381 aaaaggggaa gagaagcatg gagaatttct cactaacaga aaaccgaacc ttttcacggc
    28441 tagaaaagca aatgatctgg aagaaaccga aaagccatcg gacaagtgca gaagaatggc
    28501 ggatcgcctc cgaaatcaaa gggaattggc aagatcctga aatgaaaatc agtaacgtac
    28561 ttttggaagg agatgccggg tcggggaaga cacaattagc caaagcacta tctgctgatc
    28621 tgcaacttcc ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca gatgttttcg
    28681 gagccttatt acctgtagta gattctaata aggaacaaga ccaagaatta ctagaagcta
    28741 tttaccaaac agatacactt gaagctgtac tgacactgat ccagaaccat tacggcattg
    28801 atcctttttc tgctaaagaa aaattagcag agctagttca aaggatcgaa gctgattatc
    28861 cagatgcaat ccattacagc tattaccctt ctgaaatcgt tcgagcgatt gaaaaaggat
    28921 atttattaga gattcaagag ccgactgtca tccgagatgc ctctgttctt gtggcactta
    28981 actcagcgct cgaaccaaat gggatgttga atttaccgac aggaatcatt cgtcgccatc
    29041 cagattgcgt cattgtgatc actacaaatc gaaattacca aggaaatcgc ccattaaatg
    29101 aatctttaag agatcggatg caacatgcgg aaaaaatgga tcttcctgcc ttagaagtca
    29161 tggcagaacg tgcgatggcg aaaactatga tccaaaaaca agaacttctc ttaaagatgg
    29221 cagagattat ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa ggtgtagcag
    29281 gaatgcgttc ttatttttat tgggcaaata cctataagca aggacaagat ttactccagt
    29341 cgatttatcc aaaagttttg tataaattat ccactgattc agatgaattg gcaattcttg
    29401 aacaagcttt gtctgacagt ggattgctac tggaacttca ggacatattg agaaaagaaa
    29461 aatggggtca tcctcaatct gagcatacaa aaggaagaac cattagcgcc gaagaagcaa
    29521 atgaacgaaa catcactgaa aatgctgaag aaattctgcg cttagatgaa aagaaatcgt
    29581 tgtctgctga agaaccaaat gagggagaag agaaacaaga agaaacaata gaaaaaaacg
    29641 agattcctcc tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa aatcaaaaag
    29701 aacaacaaac aggagatgat tctcaagaaa tgtctgcttc tgacatggaa gcacatgata
    29761 aggaaatcaa gaagcaactg aataaagaag cgcgacaaat catgaaggaa acgattcatg
    29821 aaaaagaagg tctcatcgtt caccgaccag ctttccaagg aaaaacgccg gaagcacgtg
    29881 agctacacat gcaagtaatg ccaatagtag aatcacttag ccggcaaatt ttagagttac
    29941 tggataatga acaaacagag acgtaccaaa aaggaaaata cgaaggtcag cggttcaatg
    30001 caagcaaagt agcatatggt gatttgagaa attttgacaa aaagaacccg ccacatgagc
    30061 aaccttcttt agcggtagct gtacggatcg atgaatctgg gtcaatgata cgggatgatc
    30121 ggatcgaagc ggcaaaaaaa gcagcgattg ccattgccga attttctaag aaagtcgata
    30181 tcccattgtt gatttacgga gatacagcag accgttcgtc aagagaaaag acttctttgt
    30241 tttcttataa agaatttgaa gacggatttc aatggttaaa tgaaaaatta gtcactatga
    30301 aaccaaggca aaacaatcgt gatggtgctg cacttcgttt agcagcagaa aaattaaaca
    30361 gacaatccgc aacaaccaaa ctgttgctta atattagtga cggtcagcca aaagcgttgc
    30421 cggattatac aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa gaatatgaac
    30481 gtcaaggaat cctatttgtc tccgcagcta ttggccaaga caaagaagaa atcaagtcga
    30541 tctacggtga aagtcgtttt gtagatatta ctgacttgaa tgaatttccc aagcaaatga
    30601 ttcaactgat tgcccgctat ctatgataaa ataaagctca acgaacgcac ttagcagatt
    30661 cgttgagctt ttcgtttcta gttaccggta acaaggatac ttctaaaata gaatcggtta
    30721 taatcaagag ggaccgttaa aggagtgaaa cgatatgata caagaacaaa aatggtggaa
    30781 agaagcagta ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg gcgatggtca
    30841 aggagacatc aatgggatcc gtgaaaaact taattatttg aaagatcttg gtatcggctt
    30901 tatctggatc aatccgatct atcaatctcc atttgtcgat aatggttatg acgtaagtga
    30961 ctatcaagat atagaagaga gattcggaac gatggaagat tttgatttat tgctaaaaga
    31021 agcacatgaa ttagggatca aagtgattat ggatcttgtc atcaaccata gttcggatca
    31081 gcatcaatgg tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg attattacat
    31141 ttgggtcgac ggggtagatg gcaaagaacc gaataattgg acatcgattt tcggcggttc
    31201 tgcttgggag tattctcatg aaactggaca gtattatctg catgtttttg caaaagaaca
    31261 accagactta aattgggaaa gcgagaaatt gaaagaagaa ctattcaata tgatccgatg
    31321 gtggttggat aaagggatag atggatttcg tttagatgcg atttctcatg taaaaaaaga
    31381 tgaatattca gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg tctcaggaat
    31441 cgaagaacat ttgaccgatt tgaaacatgt ttttgaagag tacgatatca tgactgtcgg
    31501 agaagcaagt ggtgtcactg cagaagaagg accacaatgg gtagggaaag acggttattt
    31561 cgatatgata ttcgaatttg atcatatcca tatctggcag caagaaaaag aagggcaatt
    31621 agatgtcctt aaattgaaac ttgcattaag cgcttggcaa acttcattgg atggtatcgg
    31681 atggaacgct ttatatatgg aaaaccatga tgttcctcgt gccgtttcag tgtttggaga
    31741 tactcgtccc gatttttggg ctatgtctgc aaaagcaatc gctatgatgt atttcttttt
    31801 gcaaggtacg ccatttattt atcaaggaca ggaaatcgga atgacgaata tgccctttga
    31861 atcgatcgat caagttgatg cagtagattc gaaaagactt tataaaaggc tgcttgcgga
    31921 aggtaagaca agagaagaag cattggatat cattcgtgaa acaacgagag acaacagcag
    31981 gacacccatg caatggactt cggaacaata tgctggtttt tctacacatg aaccttggct
    32041 gatcactaat ccaaatacca aaacgatcaa tgtagaacaa caagaatatg aaccagagtc
    32101 ggtcctgcaa ttctataaga acatgatccg cattagacaa accaataaag gtctgatata
    32161 cggttcttat aaagaatatc ttcatgaaca tccacaactt tatgtctatg aaagatattt
    32221 agaagatgaa caatatctta ttatggtaaa cttaacagaa tcacttgctg actatgaact
    32281 tccaaaagaa gcagatcagt cctggacatt actcttgtca aattcctcta gcggagaatt
    32341 tgaagctaaa ggcatactag caccttatga agctcggtta tataaaacaa acaagtaaaa
    32401 cttcctcgtt acagttcatt agttggactt tgcgaggaag tttttcagat tgctctgtca
    32461 attactcatt tcaataattc gctctaggac caccgatata tttaggacga gagcgaaggg
    32521 ctgtcacgtg agcttgtcca agggttttca aacgaggtct gacaggtact tggacatgtt
    32581 tgatatgcat accgattgat gtgtcaccga tatcgatacc ggcttgtcca actacgtgtt
    32641 ccacttcgac aggatcattg aactggtcaa aagcagcaac agaacaagca cctccagcat
    32701 gtagtgcagg aacaacagag acaatttcga aattttttgc ttctgccact gttcgttcta
    32761 ctactaatgc tcggttcaaa tgttcacaac cttggacagc tagcgcaatt tctttaggat
    32821 ccaacaactc ttttaacgta cggatgatcc attgaccaac ttccgcactg gaattttttc
    32881 caatatgtcc tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga tcaccttttt
    32941 tcaagtgtgc ctcagccaat atgtcattaa ctatttcagt caattggtct tttaattctt
    33001 tttctgacaa cttcattttc taaaaccttc tttttcaatt aatattgctt ccgtgcttga
    33061 atacgttgaa gacttgcagt tataggaaag gcaagtccca ttccaatcag attttggaag
    33121 atgtttcctg gtatagaagc cactccagcc ggccaatcat ataaaatcca accggcgata
    33181 ccatagccga tgatcataac taagcttcct aataaaagcc ctaatcccca catacgagta
    33241 gaagatttgc tgaaagaacc agcaacatat ccttgtaacc cgtggatcac taatgaaaaa
    33301 actgcccact gcggatatcc tgaaatcaaa tcgatcaata gaccactgga agcaccaaca
    33361 actaatccac ccgttggacc ataaagaaaa gcagcggtat agatcccggc ctcgcataat
    33421 gtcacaaatc cattcgtagc cggtacaggg atcaaaattg ttaatgaaag ggcaactgtc
    33481 aacgcaatca gcatagcata gatcgttaat tgcttagttt tcatttctca ttcctccttt
    33541 ttgaataccg ccagaccaga cacttccggt atgatcgttc atcagcacgc cattagaaat
    33601 acaatgggca acaaattttt tactttctgc tactgctttg gtaaaatctg cccctaacac
    33661 aagattcgct gttattgcag aagaaaaggc acaaccggcc ccgtttactg ttgtagtgtc
    33721 gattttttta tgagaaaata cctcataatg atcaattgaa tagagaagat ccacagcttc
    33781 atctccagcc atccttgtgc cgcctttgat cacaatgttt tttgttcctg aagcagtgag
    33841 ttctttagcg agatggatca tttctgttaa cgaagaggga agagcttgac cagaaagtaa
    33901 agcagcttct gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt cttcgatgag
    33961 ctgcgaaata tacaattgat tgatcacagc ttcagtttct ttgaatgcca aaatagggtc
    34021 taaaatgatt gggatatttt gttttgtttt tatgaagtct cgaacaacca tgattgcttc
    34081 acttgaattc aataacccaa ttttgatacc atctaatgaa gtcattttat caatcgaaac
    34141 taactgtcgt ttaagcaatt ccaaagatat agattccagc ataaatcgct cattaatatc
    34201 tagtgcccct atagaagtca aggcgcttag tccgaataga ccaaaattct caaaagtttt
    34261 taaatctgtt tggattcctc caccagcaaa tggatcagaa ccgccaattg tcaagattct
    34321 tttcataaca ttttcctcca tgaataagag tataacgaaa ttttcaaaaa agaaaaacag
    34381 ccaattggca tatttttatc tcatccaatt taaaaaaaca aaaagaaaag aaagaccaaa
    34441 atggatcttt ctttccttaa atagttagga cttttgtgat tttaattctt tctcgatttg
    34501 aagccgttgc actttcattg tagctgttcg cgggattgca tcaaaatcac ggatgatagg
    34561 gacgttcaag cgaggaagtt catggacttg ctcccaccat cgattccaat ccatttcttt
    34621 atcaggagct aaagcaataa tcggttgtgg agaatgattt ttatccctta caatcactac
    34681 ttctgccaaa aaatctaacg aatctaataa aaagtcttca atagctaatg tactattgat
    34741 cgtttcgatc aaatccactt gccgatcttt caaaaatagg tggccatgct tatccataaa
    34801 accgtaatct ccgctatccc accaatcgcc ataaacattt tcctggaacc gtgcatcttc
    34861 cttgtaataa gtcaaagccc gtcctttaga cagtaattgt atatgaccat ctgtgttttc
    34921 aggtaggaca ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt ccagtccaac
    34981 gcccatatct cttgcatcac ttgttttcaa actctctagt gtatgagcct taagaatcat
    35041 tggtccgcat tcactttggc catatacttg aagaaatacc gcatcctgtg ccatcgacgt
    35101 tcgcaaaaat gtcttcattg tctggttgtt tatagcatca aaagtggagt gatagtatcg
    35161 gatacccgaa aaagcttctg gatgctcctt agcagtaaaa cgccactgaa caaagttatt
    35221 cggatgggtc tctagtgcaa ttggccggtg agcttccaac atatgaacaa cttttgaact
    35281 ttgtgcgttt gctaaaggca tcataggaaa tcccatggac atcaaagaag agataccaat
    35341 attgaatctg gaatgcactg gagagatgtg aaaagcgatt aatttctttt ccgaaatttt
    35401 tgtgaaaact gttttttgcc atttcgtccg ccatcccata gaatgagcag aatgacaaat
    35461 aagtttaggg ataccagttg taccagaagt atgggtcata taagaaattt catttttgtc
    35521 taaaaatgat tgagaaacag acaaagcagg ttgttccatt aaataaagaa tagga
//
LOCUS       DAEGIQ010000016        34788 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000016 DAEGIQ010000000
VERSION     DAEGIQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.016"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            160..840
                     /locus_tag="KMT45_000737"
     CDS             160..840
                     /locus_tag="KMT45_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBE7004220.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="KMT45_000738"
     CDS             930..1964
                     /locus_tag="KMT45_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBE7004221.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="KMT45_000739"
     CDS             1978..2313
                     /locus_tag="KMT45_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004222.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="KMT45_000740"
     CDS             2297..4777
                     /locus_tag="KMT45_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004223.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="KMT45_000741"
     CDS             4833..6392
                     /locus_tag="KMT45_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7004224.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="KMT45_000742"
     CDS             6389..7753
                     /locus_tag="KMT45_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBE7004225.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="KMT45_000743"
     CDS             7924..8994
                     /locus_tag="KMT45_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004226.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="KMT45_000744"
     CDS             9009..9677
                     /locus_tag="KMT45_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004227.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="KMT45_000745"
     CDS             9697..10275
                     /locus_tag="KMT45_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE7004228.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="KMT45_000746"
     CDS             10676..11587
                     /locus_tag="KMT45_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBE7004229.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="KMT45_000747"
     CDS             complement(11965..12882)
                     /locus_tag="KMT45_000747"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBE7004230.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="KMT45_000748"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="KMT45_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBE7004231.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="KMT45_000749"
     CDS             13579..14127
                     /locus_tag="KMT45_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBE7004232.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="KMT45_000750"
     CDS             14242..16602
                     /locus_tag="KMT45_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBE7004233.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="KMT45_000751"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="KMT45_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBE7004234.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="KMT45_000752"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="KMT45_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBE7004235.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="KMT45_000753"
     CDS             19108..20007
                     /locus_tag="KMT45_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004236.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="KMT45_000754"
     CDS             20000..21262
                     /locus_tag="KMT45_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004237.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="KMT45_000755"
     CDS             21423..23321
                     /locus_tag="KMT45_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBE7004238.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="KMT45_000756"
     CDS             complement(23651..24370)
                     /locus_tag="KMT45_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBE7004239.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="KMT45_000757"
     CDS             24707..25039
                     /locus_tag="KMT45_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004240.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="KMT45_000758"
     CDS             25026..27029
                     /locus_tag="KMT45_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBE7004241.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="KMT45_000759"
     CDS             27048..27518
                     /locus_tag="KMT45_000759"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBE7004242.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="KMT45_000760"
     CDS             27539..28123
                     /locus_tag="KMT45_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBE7004243.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="KMT45_000761"
     CDS             28138..29124
                     /locus_tag="KMT45_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBE7004244.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="KMT45_000762"
     CDS             29114..29425
                     /locus_tag="KMT45_000762"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBE7004245.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="KMT45_000763"
     CDS             29447..31228
                     /locus_tag="KMT45_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBE7004246.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="KMT45_000764"
     CDS             31221..32597
                     /locus_tag="KMT45_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBE7004247.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="KMT45_000765"
     CDS             32604..33239
                     /locus_tag="KMT45_000765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBE7004248.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="KMT45_000766"
     CDS             33269..34624
                     /locus_tag="KMT45_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBE7004249.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        11500 a         5988 c         7458 g         9842 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataa
//
LOCUS       DAEGIQ010000017        34660 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000017 DAEGIQ010000000
VERSION     DAEGIQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.017"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(313..2205)
                     /locus_tag="KMT45_000767"
     CDS             complement(313..2205)
                     /locus_tag="KMT45_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004250.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="KMT45_000768"
     CDS             complement(2202..3929)
                     /locus_tag="KMT45_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004251.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLSSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="KMT45_000769"
     CDS             complement(3945..4595)
                     /locus_tag="KMT45_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBE7004252.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="KMT45_000770"
     CDS             4799..5095
                     /locus_tag="KMT45_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBE7004253.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="KMT45_000771"
     CDS             5287..5907
                     /locus_tag="KMT45_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBE7004254.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="KMT45_000772"
     CDS             complement(5999..7165)
                     /locus_tag="KMT45_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE7004255.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="KMT45_000773"
     CDS             complement(7177..7530)
                     /locus_tag="KMT45_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBE7004256.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="KMT45_000774"
     CDS             7658..8680
                     /locus_tag="KMT45_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBE7004257.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8699..8860
                     /locus_tag="KMT45_000775"
     CDS             8699..8860
                     /locus_tag="KMT45_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004258.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9119..9553)
                     /locus_tag="KMT45_000776"
     CDS             complement(9119..9553)
                     /locus_tag="KMT45_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004259.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="KMT45_000777"
     CDS             complement(9663..11522)
                     /locus_tag="KMT45_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBE7004260.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12799)
                     /locus_tag="KMT45_000778"
                     /pseudo
     CDS             complement(11640..12799)
                     /locus_tag="KMT45_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13106..14530)
                     /gene="araA"
                     /locus_tag="KMT45_000779"
     CDS             complement(13106..14530)
                     /gene="araA"
                     /locus_tag="KMT45_000779"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBE7004261.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14546..15244)
                     /locus_tag="KMT45_000780"
     CDS             complement(14546..15244)
                     /locus_tag="KMT45_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBE7004262.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15201..16862)
                     /locus_tag="KMT45_000781"
     CDS             complement(15201..16862)
                     /locus_tag="KMT45_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBE7004263.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17080..18480
                     /locus_tag="KMT45_000782"
     CDS             17080..18480
                     /locus_tag="KMT45_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBE7004264.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18732..19811)
                     /locus_tag="KMT45_000783"
     CDS             complement(18732..19811)
                     /locus_tag="KMT45_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7004265.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19897..20856)
                     /locus_tag="KMT45_000784"
     CDS             complement(19897..20856)
                     /locus_tag="KMT45_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBE7004266.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20868..22379)
                     /locus_tag="KMT45_000785"
     CDS             complement(20868..22379)
                     /locus_tag="KMT45_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBE7004267.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22401..22964)
                     /locus_tag="KMT45_000786"
     CDS             complement(22401..22964)
                     /locus_tag="KMT45_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBE7004268.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22970..23833)
                     /locus_tag="KMT45_000787"
     CDS             complement(22970..23833)
                     /locus_tag="KMT45_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7004269.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23847..24743)
                     /locus_tag="KMT45_000788"
     CDS             complement(23847..24743)
                     /locus_tag="KMT45_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7004270.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24918..26207)
                     /locus_tag="KMT45_000789"
     CDS             complement(24918..26207)
                     /locus_tag="KMT45_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE7004271.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26179..27696)
                     /locus_tag="KMT45_000790"
     CDS             complement(26179..27696)
                     /locus_tag="KMT45_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBE7004272.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27894..28784
                     /locus_tag="KMT45_000791"
     CDS             27894..28784
                     /locus_tag="KMT45_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBE7004273.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="KMT45_000792"
     CDS             complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="KMT45_000792"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBE7004274.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29693..31165)
                     /locus_tag="KMT45_000793"
     CDS             complement(29693..31165)
                     /locus_tag="KMT45_000793"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBE7004275.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31802..32164)
                     /locus_tag="KMT45_000794"
     CDS             complement(31802..32164)
                     /locus_tag="KMT45_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBE7004276.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32387..33139
                     /locus_tag="KMT45_000795"
     CDS             32387..33139
                     /locus_tag="KMT45_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBE7004277.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33279..34202)
                     /locus_tag="KMT45_000796"
     CDS             complement(33279..34202)
                     /locus_tag="KMT45_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7004278.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34217..34489)
                     /locus_tag="KMT45_000797"
     CDS             complement(34217..34489)
                     /locus_tag="KMT45_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004279.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10229 a         7212 c         5980 g        11239 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gaggacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    12061 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    12121 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    12181 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12241 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12301 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12361 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12421 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12481 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12541 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12601 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12661 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12721 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12781 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12841 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12901 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12961 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    13021 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    13081 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    13141 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    13201 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13261 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13321 tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt
    13381 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13441 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13501 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13561 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13621 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13681 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13741 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13801 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13861 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13921 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13981 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    14041 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    14101 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    14161 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    14221 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14281 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14341 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14401 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14461 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14521 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14581 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14641 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14701 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14761 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14821 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14881 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14941 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    15001 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    15061 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    15121 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    15181 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15241 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15301 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15361 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15421 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15481 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15541 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15601 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15661 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15721 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15781 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15841 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15901 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15961 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    16021 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    16081 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    16141 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    16201 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16261 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16321 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16381 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16441 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16501 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16561 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16621 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16681 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16741 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16801 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16861 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16921 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16981 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    17041 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    17101 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    17161 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    17221 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17281 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17341 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17401 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17461 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17521 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17581 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17641 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17701 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17761 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17821 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17881 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17941 gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg
    18001 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    18061 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    18121 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    18181 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18241 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18301 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18361 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18421 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18481 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt
    18541 cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc
    18601 agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg
    18661 atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa
    18721 aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca
    18781 tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca
    18841 tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg
    18901 cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt
    18961 tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct
    19021 aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc
    19081 gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg
    19141 ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga
    19201 cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct
    19261 aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg
    19321 ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta
    19381 ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc
    19441 aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca
    19501 ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca
    19561 ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat
    19621 gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc
    19681 cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt
    19741 gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga
    19801 tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc
    19861 aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc
    19921 accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt
    19981 aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaaat
    20041 atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt
    20101 tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag
    20161 attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc
    20221 agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt catttactga
    20281 aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa
    20341 cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc
    20401 ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact
    20461 gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc
    20521 tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt
    20581 atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc
    20641 ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc
    20701 atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac
    20761 cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg
    20821 ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt
    20881 attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct
    20941 attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc
    21001 aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt
    21061 tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg
    21121 ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa
    21181 acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc
    21241 atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt
    21301 attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata
    21361 gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg
    21421 ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca
    21481 tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta
    21541 gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta
    21601 tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag
    21661 ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat
    21721 tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca
    21781 tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc
    21841 ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca
    21901 aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa
    21961 ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg
    22021 aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa
    22081 tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct
    22141 acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct
    22201 aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt
    22261 tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag
    22321 ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact
    22381 tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt
    22441 ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca
    22501 aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat
    22561 gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc
    22621 caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg
    22681 attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta
    22741 aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt
    22801 cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg
    22861 atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa
    22921 accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca
    22981 ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta
    23041 atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc
    23101 ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca
    23161 atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag
    23221 taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa
    23281 tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga
    23341 taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa
    23401 gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca
    23461 gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc
    23521 ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat
    23581 ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat
    23641 agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag
    23701 aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg
    23761 ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc
    23821 tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac
    23881 acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat
    23941 gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt
    24001 tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc
    24061 gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta
    24121 atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag
    24181 ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa
    24241 acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc
    24301 ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata
    24361 gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga
    24421 caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata
    24481 atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct
    24541 cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag
    24601 aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat
    24661 aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc
    24721 caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca
    24781 atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa
    24841 cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc
    24901 ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc
    24961 atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg
    25021 ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt
    25081 ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc
    25141 aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa
    25201 tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca
    25261 tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta
    25321 gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt
    25381 tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct
    25441 ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca
    25501 ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac
    25561 atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta
    25621 ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt
    25681 tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt
    25741 acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct
    25801 ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct
    25861 ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt
    25921 ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt
    25981 tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt
    26041 tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca
    26101 gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa
    26161 gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca
    26221 tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt
    26281 tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt
    26341 atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg
    26401 aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc
    26461 atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt
    26521 tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa
    26581 tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat
    26641 aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt
    26701 gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg
    26761 aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt
    26821 ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc
    26881 gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg
    26941 agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc
    27001 gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga
    27061 acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc
    27121 ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat
    27181 ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct
    27241 taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc
    27301 atctactcca cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca
    27361 ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc
    27421 caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac
    27481 aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac
    27541 gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata
    27601 tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac
    27661 acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt aatttataca
    27721 tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt
    27781 ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt taatataact
    27841 tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac
    27901 tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca
    27961 agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg
    28021 gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca
    28081 aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca
    28141 gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc
    28201 actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg
    28261 ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct
    28321 ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat
    28381 tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt
    28441 ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg
    28501 gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc
    28561 aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac
    28621 ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa
    28681 tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg
    28741 gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat
    28801 tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat
    28861 ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac
    28921 catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca
    28981 atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca
    29041 agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat
    29101 tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca
    29161 aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct
    29221 gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct
    29281 ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca
    29341 gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt
    29401 gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt
    29461 tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa
    29521 gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc
    29581 aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt
    29641 ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt
    29701 ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc
    29761 agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt
    29821 cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt
    29881 cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc
    29941 gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc
    30001 gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag
    30061 tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact
    30121 tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac
    30181 caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt
    30241 tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga
    30301 tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg
    30361 ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg
    30421 ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca
    30481 cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt
    30541 ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta
    30601 cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat
    30661 ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt
    30721 tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg
    30781 tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa
    30841 aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat
    30901 actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat
    30961 tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc
    31021 cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac
    31081 ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac
    31141 tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct
    31201 acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca
    31261 catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt
    31321 attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga
    31381 agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat
    31441 cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta caacataatc
    31501 aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag
    31561 aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat
    31621 accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta
    31681 agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt
    31741 gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt
    31801 attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt
    31861 tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt
    31921 ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt
    31981 tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg
    32041 ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca
    32101 ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct
    32161 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
    32221 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
    32281 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
    32341 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
    32401 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
    32461 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
    32521 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
    32581 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
    32641 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
    32701 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
    32761 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
    32821 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
    32881 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
    32941 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
    33001 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
    33061 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
    33121 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
    33181 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
    33241 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
    33301 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
    33361 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
    33421 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
    33481 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
    33541 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
    33601 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
    33661 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
    33721 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
    33781 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
    33841 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
    33901 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
    33961 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
    34021 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
    34081 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
    34141 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
    34201 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
    34261 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
    34321 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
    34381 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
    34441 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
    34501 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
    34561 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
    34621 ttataatgat actgtaaata tatctttatt attagtgaat
//
LOCUS       DAEGIQ010000018        34500 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000018 DAEGIQ010000000
VERSION     DAEGIQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.018"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            214..498
                     /locus_tag="KMT45_000798"
     CDS             214..498
                     /locus_tag="KMT45_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBE7004280.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="KMT45_000799"
     CDS             complement(779..1198)
                     /locus_tag="KMT45_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBE7004281.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="KMT45_000800"
     CDS             complement(1200..1784)
                     /locus_tag="KMT45_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBE7004282.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="KMT45_000801"
     CDS             complement(1831..3306)
                     /locus_tag="KMT45_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBE7004283.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="KMT45_000802"
     CDS             3487..3798
                     /locus_tag="KMT45_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004284.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="KMT45_000803"
     CDS             complement(3913..6249)
                     /locus_tag="KMT45_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7004285.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="KMT45_000804"
     CDS             6391..7059
                     /locus_tag="KMT45_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7004286.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="KMT45_000805"
     CDS             7062..8105
                     /locus_tag="KMT45_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBE7004287.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="KMT45_000806"
     CDS             complement(8342..9223)
                     /locus_tag="KMT45_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE7004288.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERALRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="KMT45_000807"
     CDS             9382..10110
                     /locus_tag="KMT45_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBE7004289.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKSCGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="KMT45_000808"
     CDS             10266..10583
                     /locus_tag="KMT45_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBE7004290.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="KMT45_000809"
     CDS             10576..11184
                     /locus_tag="KMT45_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBE7004291.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="KMT45_000810"
     CDS             11239..12030
                     /locus_tag="KMT45_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBE7004292.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="KMT45_000811"
     CDS             12168..12656
                     /locus_tag="KMT45_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBE7004293.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="KMT45_000812"
     CDS             12707..13579
                     /locus_tag="KMT45_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBE7004294.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="KMT45_000813"
     CDS             13755..14117
                     /locus_tag="KMT45_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBE7004295.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYIQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="KMT45_000814"
     CDS             14126..14878
                     /locus_tag="KMT45_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004296.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="KMT45_000815"
     CDS             14892..16694
                     /locus_tag="KMT45_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004297.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="KMT45_000816"
     CDS             16971..18011
                     /locus_tag="KMT45_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBE7004298.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="KMT45_000817"
     CDS             complement(18063..20207)
                     /locus_tag="KMT45_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBE7004299.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="KMT45_000818"
     CDS             complement(20564..21937)
                     /locus_tag="KMT45_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBE7004300.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="KMT45_000819"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="KMT45_000819"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBE7004301.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="KMT45_000820"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="KMT45_000820"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBE7004302.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="KMT45_000821"
     CDS             complement(23385..24398)
                     /locus_tag="KMT45_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBE7004303.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25081)
                     /locus_tag="KMT45_000822"
     CDS             complement(24479..25081)
                     /locus_tag="KMT45_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBE7004304.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITNYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25266..25967
                     /locus_tag="KMT45_000823"
     CDS             25266..25967
                     /locus_tag="KMT45_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004305.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25977..28688
                     /locus_tag="KMT45_000824"
     CDS             25977..28688
                     /locus_tag="KMT45_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004306.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANIGNGSVDYFVYLPESAFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29034..30662
                     /gene="treC"
                     /locus_tag="KMT45_000825"
     CDS             29034..30662
                     /gene="treC"
                     /locus_tag="KMT45_000825"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBE7004307.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30722..31903)
                     /locus_tag="KMT45_000826"
     CDS             complement(30722..31903)
                     /locus_tag="KMT45_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE7004308.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32155..33678
                     /locus_tag="KMT45_000827"
     CDS             32155..33678
                     /locus_tag="KMT45_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBE7004309.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33747..34475)
                     /locus_tag="KMT45_000828"
     CDS             complement(33747..34475)
                     /locus_tag="KMT45_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7004310.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT        10900 a         6177 c         7004 g        10419 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctaag ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaatctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatatacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaag gcacttggat tgtagtttgt gatagtcaac atattgtttg
    24781 tatctttcaa ggaatccttc aacaacaagt cagtgaattc tgccggagca gacaaagcag
    24841 atattcctcc agcatctgtc tgatgactta gataagagaa agtagtgatg tctacattca
    24901 ctaaaacttt caaggcagcg gtacgtttca atgtttttgc taacaaatca ttgtttgtca
    24961 gaatagattg tgcagtgtgt ggagagagtg taccgttatt tacgatccct ttttgcatgt
    25021 cgatcgcgac aaaagcagtt tttgataaat caaaaatcat attttccttt aaatcattca
    25081 tgatgttcct tctttcaaat aacaatgttt tttatgtaag taaatcataa atcgtttatt
    25141 tgtcaactaa ataaaggatt cctttcttta gtctagagaa attcagtggt aaaataaata
    25201 agtgtgagaa aacgcattcg atgcatagat atagcgagaa atatcgtgaa aatggaggaa
    25261 tcagagtgaa gtatgtcgaa gtaaaaaatg aatacaaacg atacaaaatg ggagaaacga
    25321 ccattacagc aaatgacgat atttcttttt caattgaaaa aggcgaatta gtgattatac
    25381 ttggtccaag tggagcagga aagtcaacga ttcttaatat tttaggtggg atggatacac
    25441 ctgatgaagg tcagatcatt attgatgaca cagatattgc gcaattttca gataaacagc
    25501 ttactgctta ccgacgaaca gatgtagggt tcgttttcca attctacaat ctcgttccta
    25561 atctaacagc aaaagaaaat gtggaattgg caacggaagt ttctcctgat gcgcttgatc
    25621 ctgtagaggt attgaggcaa gtgggcctag ctcatcgatt gaataacttt ccgtcccaat
    25681 tatcaggagg cgaacagcag agggtttcaa ttgctcgtgc actagctaaa aatcccaaac
    25741 ttcttttatg tgatgaaccg acaggcgcgc tggattttga aacgggcaag caagtattga
    25801 aattattgca aaacgctagc agacaacacg gcaataccgt tttgatcatt acacataatt
    25861 ctgctttagc gcccatcgct gatagagtca ttcacattaa tgatgctaaa gtaaggtctg
    25921 ttgaattaaa tgatcatcct tcatccattg atgagattgt atggtagggg gattttatga
    25981 aaacaagaac atatcttaaa gcaggttttc gcgaaattcg gcaatctaaa ggacgattca
    26041 ttgcaatcat ccttattgtc atgctaggaa cacttttatt tgtaggtgtc aaaactgctg
    26101 gacctgtcat gcaaaaaacg atggatcact atgttggtac agcaggactt tctgatttgc
    26161 aaatcgtttc tactggtggc ttgacacaaa aagatattgc tcaagcagaa aagatcccag
    26221 atgcaaaagt agaaacagga aaacagatct attattccaa tactggcaaa aacgaagtga
    26281 ttcaaatatt ttcttataac aaaaataaca agcagaacaa gctacaatta actgatggaa
    26341 aactcccgga aaaaccaaac cagcttgtat tggatgaaaa agcggaagat gaagggtata
    26401 aaatcggtga tatttatcag attgattcag atgaattgaa agaaaaagag tataccatca
    26461 cgggctttgt ccgttctcca ttgttcatca acaatttgga gagaggatac gcgaacatcg
    26521 gaaatggtag cgtcgattat tttgtctatt tgcctgaatc cgcttttaaa acagatattt
    26581 attctgtctt atatcttgat tttacaaacg taaagaattt gaatacgtac agcaaagctt
    26641 acaaagataa aatggagcaa aatcaagaaa aggcagaaaa gtatttacaa gatcgacccg
    26701 aagaacgctt agaagagctg aaaaaatcgg ccaacgaaga attagaacca gcacagcaaa
    26761 aaataacaga aggcaaagca caaatagcac agggaagaac acagttagaa gatgctaaaa
    26821 aacagttagc gcaacagtca gcagctatcc agcagcttcc tgaagcgcaa cgaacaatcg
    26881 ctgttcaaac attatctcaa caagaagctc aacttgctga acaagaaagg caattagttg
    26941 agaaagaacg tgaactggcc tctgcagagg aagaattgaa tcgaaatgca gaaaaaattg
    27001 aaaatttaac taaaccatca tacctataca atgaacgttc agaaaatgtc ggttttcaag
    27061 aatttggtga tctgtctgat cgtatcgctg cgattgctaa tgtttttcct gttttctttt
    27121 tctttattgc cgcattgatc acctttacga caatgacacg tatggtagaa gaaaaccgca
    27181 gggaaatcgg gacattaaag gcgttaggtt atacgaaagt tgaaatcgca aaaaaatatg
    27241 caatctatgc aagtctagcc tcagggatag gcattgtttt aggaacgata ttaggaacga
    27301 atcttttgcc aaggatcatt tttgagctgt ctaacgagcg gtatgatatt gggagtgcgc
    27361 ttatttttta tgactggccg ccgattttac aagcagctgc cgcattcttc attgccgcat
    27421 ttggcgctgc aatgatcgtt ttattcaaag atttaagaga acggcctgct gcattgcttc
    27481 aaccgaaagc accgaagcca gggaaacgca tttttcttga atacatcact ccgctgtggt
    27541 cacgccttag ctttaatcaa aaaatcagtt accgaaatct cttccgatat aagtcaagaa
    27601 tgcttatggc gattatcgga attgctggat gtgccgggct gatggttgca ggcgtgggat
    27661 taaaagattc tctaagttct gtctcagcca aacaatttgg accgatcatt gactatcaag
    27721 caatcgttac attggattca tccacggaag atagtcgtca aaaagcagcg gatatgctgc
    27781 agaagcaaga aaaagtaaat gagatcctag ctattcatac tcaaaccgta gaattgagaa
    27841 aaaaaggaca tgcttcgcaa agtctgacag tgatcgtacc aagtaaaaca gctgatttgg
    27901 atcgttttat ccatttaact gatcttgaag agaacccacg ctccttgcct aaaacaggaa
    27961 tcgctattac gcaaaaagca gcagaactat ttgacttatc tacaggagat catatttcgc
    28021 tttatgatga agatcaaaaa gaatggaaag tcgaagtaaa agaaatcgtg aaaaactatc
    28081 taggtagctt tatttatatg tcccctgaat attatgcttc agttactgat caatcgatga
    28141 tggataatgc atttttgctg cgtacagata agatgacaac gaaagaagaa gaaatgcttt
    28201 ccgagaaact gcttgccaca gacgctgtga caaatacgtc tttcatgtcc aaacaaatcg
    28261 aaacgcaaga agaatcaatg gcgaacttag actcggttgt cttaatcttt gtcgtattat
    28321 caggactgct ggcttttatc gtcttgtata atctaactaa tatcaatatt tccgagcgtg
    28381 tacgcgaatt atctacaata aaagtccttg gattttttga taaagaagtc acgatgtaca
    28441 ttgttcgaga aaatatcatc tttactcttt taggaatcat cggaggattt ggtgtaggat
    28501 atatattgac tgacttcatc ttgcaacaag cgtctatgga gaatgtcatc tttccattag
    28561 tgatcacatg gtgggcttat gtcttatcag caggattaac tatcgtattc acggttattg
    28621 tgatgatcgt tacccatttc aaattaaagc atatcgatat gattgatgcg ttgaaatcga
    28681 atgaataaaa acagaggttg tgaaatcagc ggttgtgaaa aagctgtcct gcatcgagca
    28741 aaaagaatac tcaaataaaa ttagctcctc atgttttaca tttgagaaga ctgattgccg
    28801 tagataaaag cttttgaaca ccatttatag aagaaacaag aatattttgc tattttaaac
    28861 aagaggatag acaaatacat gtctgatctc ttgtttttta ttgggcaaca gacctcttag
    28921 aatgtataaa gataaatttt acaaacgctt tcggttcagt tataataata aatgataact
    28981 tgtatatata agttgtggta ggtaataaat cagttgaatg gaggtaaata tccatgacat
    29041 ttaaagacaa agtcatttac caaatctatc ccaaatctta caaagacagt aatggtgatg
    29101 gaattggaga tttaaaagga atcaaagaaa aactcccgta tctgaatgat ctaggcatcg
    29161 acatgatttg gctaaaccct atttatccaa gccctcaaaa agacaacggt tatgatatct
    29221 ctgattacac agccatcgat cctatctttg gtacgatgga ggaattcgaa cagctaatag
    29281 ccgaagcaaa gaaataccgg atagaaatca tgctcgatat ggtgctgaat cacgtttcga
    29341 cagaacatga atggttcaaa aaagcactag caggggacca gcattatcag gacttcttca
    29401 ttttaagaga agagccaacg gattgggtat caaaatttgg tggaagtgct tggtcgccat
    29461 ttggagatac ggggaaatat tatttgcact tatatgatcg gacacaagct gacttgaact
    29521 ggcggaacca agcagtaaga gaggaactat tcaaagttgt tcgtttttgg atggaaaaag
    29581 gggtaaaagg attccgtttc gatgttatca atgttatcgg taaagacgaa caattgaaaa
    29641 ataataaaga aaatgatggc aaacctgaat atacggacaa accaatcacc catacgtatc
    29701 taaaagaatt gaaccaagca acgtttggga cggattccac tatcatcaca gtcggtgaaa
    29761 tgagctctac gacgatcgaa aattgtatct tgtatacaga acctagtaga gaagaattat
    29821 caatggtctt taatttccat catttgaaag tcgattatga aaatgggaat aaatggtcta
    29881 ctcctccatt tgattttgca gaattaaagc gactatttca tacatgggga gaaaagatga
    29941 gtgagggtaa cggctggaat gctctatttt ttaataatca tgatcagcca cgtgcgctca
    30001 atcgatttgt agctatcgat cagtatcgaa aacaaggagc agagatgcta gcaaccatga
    30061 tccatttgaa ccgtgggacg ccctatattt atatgggaga agaaatcggc atgatcgacc
    30121 cagactattc atcgatttcc gactatattg acgtagaaag tttgaatgct tataagttac
    30181 tccttgaaca aggattcaca gaaaaagaag cgtttcgtcg gatcaaagca aaatctcgcg
    30241 ataactctcg aacacccatg caatggtcag acacagaaca agcaggattt actacaggga
    30301 aaccttggtt aaaagttgga ggcaagctag aagagattaa tgtagaaaaa gaactatctt
    30361 ctgaagattc tattttgtcc tattacaaga aattgattcg gctgagaaaa acatatccaa
    30421 tcgttgcaca aggagattat catgcttatg aagcaaatca tccggaagtt tatggctttc
    30481 ttcgccaatt tgaaggacag cagctgcttg tattaacaaa tttctatgat aaagatacaa
    30541 ggatcgccat cccagaagga ttcacagaag ccaatattct tatcgataat tattccgata
    30601 ctgttcttca tcaaagtctg gagctaaaac cataccaggc aattgctttg ctgaaaaatt
    30661 aaaacaaaaa cacaaaacgt cgttcctacg agctattctc gtaagatcga cgttttgtgt
    30721 tttatttaaa ataatcttct tcagaaaatg aagagttttc atactctcct ttatcgccat
    30781 aaaatttatt gtagcgttgt ttgaatttac ggaaaataaa agtaacgagt aatttgacta
    30841 acgtatagat aggaatacca aaaataacgc ccacaattcc cattagatca cccatgacaa
    30901 gtaaaacgat taagatcgta atcggatgga tctgtaaacg atcacccatt actcgaggaa
    30961 tcaccaagtc accgtggaga agttgtacgg caaaccagac aatgacaaat ttgacagcca
    31021 taaaagtaga gtcttggaat gcaatgatca aacctggaaa gaaggcgata aacggcccaa
    31081 tatatggaat gatacataac aacccagcag aaatagctaa aacactagca aattctaatc
    31141 cgatcagtaa gaaacaaacc cagtagactg ctccaaggat aaatgaagca atgatttgcc
    31201 cctttagaaa tgaacccaat tgttgattag caatctttgt caaattttta aaatctgtac
    31261 gaaatgctgg tgggacaatc gctaaaactg actgataaaa tttttgagga tctctcaaaa
    31321 ggaaaaaagc gacaattgga ccagtaaata aagtaataaa aatagctgta acagttgaaa
    31381 aaatgttgct gatccgttgt gcgccacttt cccaataatt tccaataaat ccagctatat
    31441 cagacgtcac gtcgtcaata gaagcgaaaa attgattaaa tgaatcggca aaaggtgttt
    31501 tctccaaaaa ttgtcggaga ctctcttgaa aatcttgaaa caaatctggc aaactactga
    31561 tcaatgtctt tgcctgttct tcaagaaaag gaataagcca tatgcttcca agccccaata
    31621 aaaataaaat aatcacataa acgataatga ctgacaagtt cctcgaaagg tgcttgctca
    31681 aacgtttgat gactggttcc aaaagataat acaagatcag tgcaaacaat aatgggggca
    31741 agatagtagc gataatgatt ccgacaggct tgaatataaa cgagatctga tggaaaaaat
    31801 aaatgatcag cgcaagtaag ataactattc ctaaaacata gtaagaggtt tttcctccta
    31861 aaaagcggat caaacgattc gttgtatctg tttctttttt cactcttttt cctcctttat
    31921 ctataacttc attatagaca aaagcgggta taaaaggaaa atgatagagg ttgattgcaa
    31981 aaatatcgtc aatcattcat ataaactttc tcatcaatta aaataagaaa acgcttgaca
    32041 gttcttttaa ccatatgata aattaaatcc tgttgctaaa acgtaactat tacggtttgc
    32101 gaaaagcgaa tcggaacatt caaatgaaag aagatgagaa gaggagtgtc ataaatgaaa
    32161 cgattgatag gtatattggc catgctggtt ttagcaggta tgttaactgc ttgcggggca
    32221 agtgggaaag ccgaggacag taaagaaaaa ttgagcgtga tgacgacctt ttatcccatg
    32281 tatgatttta caaaagcaat cgtcggggat gaaggagaag tagagttatt gattccagca
    32341 gggactgatt cccacgatta tgaaccttct gctaaagaca tggcaaagat ccaagataca
    32401 gatatctttg tttataatga tgaaaacatg gaaacatggg tccccgcaat ccaaaaaaca
    32461 ttacaagaag gcaatgtcca tacgataaaa gcgacagaag gtatgctttt acttccggga
    32521 agtgaagaag gacatgatca tgaccatgaa cacggggaag aaggccatac acatgagcta
    32581 gacccacatg tatggctagc tccaagttta gcaatcaagc aggtagcaaa catacgtgat
    32641 cagctgattg aagcttaccc agaaaaacag gaagtatgga caaaaaatgc agcagcctat
    32701 accgagaaac ttcaagcact ccatcagctt tatcaagaaa cctttaagca agcaaaacaa
    32761 agatcttttg tcacgcaaca tgctgcattc aactatctag cgttagaata tggattgaat
    32821 caagtatcga ttgctggtct ttcttctagt gaagaaccgt ctgcggcgag aattgctgaa
    32881 ctgaaacact ttgttaaaga acatggaatc aactatattt attttgaaga aaacgccaaa
    32941 gatagtattg ccagaacgtt agcaaacgaa gcaggtgtat ctttagaagt attgaatcct
    33001 ttggaaggat tgacgaatga acaaatagaa aatggagaaa actatctttc tatcatggaa
    33061 gcaaacctcg aagcgttgaa aaaaacgaca gagacagaaa acccgctaga agatactttg
    33121 acccccaaaa aagaaaagag cgtctacaac ggttattttg aagacgagga tgtacaagat
    33181 cgctctcttt cagattgggc aggaacttgg caaacagtag atacgtatgt agaagatggg
    33241 acttttgatc ctgtttttga atataaagaa aaattgaatc aggataagac agcacaagaa
    33301 tataaagaat attatacgaa gggctatcaa acagatattg cttcaatcaa gatcagtgag
    33361 aaagagatgg catttacgtt taagaatgga acaacgaaac gttcaaatta tcgttatgta
    33421 gggaaagaaa tcttgaccta cgaggcagga aaccgtggcg taaggtatct attcgaagca
    33481 gaagataagg aaagtggcgc ttttagatat attcaattca gtgatcacac gattgcaccg
    33541 gctgactccg aacattttca tttatatgca ggaaatgaaa gtcaagaagt attgctgaaa
    33601 gaaatggaga attggccaac attttatcca gatacaatgg atggacgtga aattgcacag
    33661 gaaatgcttt tgcactgata ggcaaaaaag aaccgaaggg tttggatcgg ctggtcattt
    33721 agtcggtcca aactattcgg ttctttttat gaaatcaatt tctttaatcc acggatagag
    33781 gctgggatgg tctcgccgtt tgccaacgtg acttctctgg tttttttatt aatcgaccgc
    33841 acattttgtg tattgataac ataggattta tgacaacgga taaaattccg atgcattttc
    33901 tcgatttctt tgatcttctc atagaattgt aattgggcat gagtcaaatg gacttccagt
    33961 ttatgtggcg ctcctagcga agtagaaaaa tattggatgt ctttgatcgg taaaaatttg
    34021 atctcatcat taaactctat tttgaagata tctttttgga tttgggacat ttgaaaatct
    34081 tcgaaaattt tgctcaggat atgctcgaca ttttctttta aatcataaag atcttctttg
    34141 acgatataat cagtcggttc gatgttgctt tttaagattt tcatcgacat ttccgtatgg
    34201 gaagtcacat agattatttt tcctaaagga tctaattcac ggatcaattt accgatcgct
    34261 acaccatcca tttcactgtc ttctagttcg atatctaaaa agtaaatacc aaaagaagaa
    34321 gttctgatct tggctgcatt gagaagttct tccggatcag aggtagaaat atggacataa
    34381 ctgtctaagt tttcaatcat gattctattt ttgatcaccg ttgataaaac ttctaattgt
    34441 tttggatcgt cttcacaaat ataaatggga atcatatcat tactccttta ttttatttgt
//
LOCUS       DAEGIQ010000019        33919 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000019 DAEGIQ010000000
VERSION     DAEGIQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.019"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1152
                     /locus_tag="KMT45_000829"
     CDS             <1..1152
                     /locus_tag="KMT45_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBE7004311.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="KMT45_000830"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="KMT45_000830"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBE7004312.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="KMT45_000831"
     CDS             2728..3003
                     /locus_tag="KMT45_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBE7004313.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="KMT45_000832"
     CDS             3009..3776
                     /locus_tag="KMT45_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBE7004314.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="KMT45_000833"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="KMT45_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBE7004315.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="KMT45_000834"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="KMT45_000834"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBE7004316.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="KMT45_000835"
     CDS             7920..8222
                     /locus_tag="KMT45_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBE7004317.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="KMT45_000836"
     CDS             8225..9388
                     /locus_tag="KMT45_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBE7004318.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="KMT45_000837"
     CDS             9414..12842
                     /locus_tag="KMT45_000837"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBE7004319.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="KMT45_000838"
     CDS             12863..13987
                     /locus_tag="KMT45_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004320.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="KMT45_000839"
     CDS             13977..14414
                     /locus_tag="KMT45_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBE7004321.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="KMT45_000840"
     CDS             14398..14736
                     /locus_tag="KMT45_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBE7004322.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="KMT45_000841"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="KMT45_000841"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBE7004323.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="KMT45_000842"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="KMT45_000842"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBE7004324.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="KMT45_000843"
     CDS             15893..16936
                     /locus_tag="KMT45_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBE7004325.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="KMT45_000844"
     CDS             17095..17823
                     /locus_tag="KMT45_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBE7004326.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="KMT45_000845"
     CDS             17939..18184
                     /locus_tag="KMT45_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBE7004327.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="KMT45_000846"
     CDS             18181..18399
                     /locus_tag="KMT45_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE7004328.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="KMT45_000847"
     CDS             18535..19074
                     /locus_tag="KMT45_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBE7004329.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="KMT45_000848"
     CDS             19067..21340
                     /locus_tag="KMT45_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBE7004330.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="KMT45_000849"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="KMT45_000849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBE7004331.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="KMT45_000850"
     CDS             complement(22513..22767)
                     /locus_tag="KMT45_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBE7004332.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="KMT45_000851"
     CDS             complement(23167..23436)
                     /locus_tag="KMT45_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004333.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="KMT45_000852"
     CDS             complement(23906..24361)
                     /locus_tag="KMT45_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBE7004334.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="KMT45_000853"
     CDS             complement(24499..26427)
                     /locus_tag="KMT45_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBE7004335.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="KMT45_000854"
     CDS             complement(26624..27457)
                     /locus_tag="KMT45_000854"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBE7004336.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="KMT45_000855"
     CDS             complement(27649..28353)
                     /locus_tag="KMT45_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBE7004337.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="KMT45_000856"
     CDS             complement(28376..29002)
                     /locus_tag="KMT45_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBE7004338.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="KMT45_000857"
     CDS             29328..31703
                     /locus_tag="KMT45_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBE7004339.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGVYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="KMT45_000858"
     CDS             complement(31727..32218)
                     /locus_tag="KMT45_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBE7004340.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="KMT45_000859"
     CDS             32405..33325
                     /locus_tag="KMT45_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBE7004341.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="KMT45_000860"
     CDS             33479..33823
                     /locus_tag="KMT45_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBE7004342.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        11244 a         5930 c         7036 g         9709 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggatt tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccaaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggtgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcacccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt gtttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttat
//
LOCUS       DAEGIQ010000020        33412 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000020 DAEGIQ010000000
VERSION     DAEGIQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.020"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..581
                     /locus_tag="KMT45_000861"
     CDS             <1..581
                     /locus_tag="KMT45_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBE7004343.1"
                     /translation="YIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTLEQLKEAN
                     ADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYELGEEKQFG
                     EGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRVSVVIPGA
                     KRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(670..1929)
                     /locus_tag="KMT45_000862"
     CDS             complement(670..1929)
                     /locus_tag="KMT45_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBE7004344.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(2100..2546)
                     /locus_tag="KMT45_000863"
     CDS             complement(2100..2546)
                     /locus_tag="KMT45_000863"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBE7004345.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(2557..4770)
                     /locus_tag="KMT45_000864"
     CDS             complement(2557..4770)
                     /locus_tag="KMT45_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBE7004346.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(5003..5767)
                     /locus_tag="KMT45_000865"
     CDS             complement(5003..5767)
                     /locus_tag="KMT45_000865"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBE7004347.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(5768..6715)
                     /gene="prmA"
                     /locus_tag="KMT45_000866"
     CDS             complement(5768..6715)
                     /gene="prmA"
                     /locus_tag="KMT45_000866"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBE7004348.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(6728..7216)
                     /locus_tag="KMT45_000867"
     CDS             complement(6728..7216)
                     /locus_tag="KMT45_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBE7004349.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(7182..7853)
                     /locus_tag="KMT45_000868"
     CDS             complement(7182..7853)
                     /locus_tag="KMT45_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBE7004350.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            7991..9277
                     /locus_tag="KMT45_000869"
     CDS             7991..9277
                     /locus_tag="KMT45_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBE7004351.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            9349..9543
                     /gene="ssrS"
                     /locus_tag="KMT45_000870"
     ncRNA           9349..9543
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KMT45_000870"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            9621..9905
                     /locus_tag="KMT45_000871"
     CDS             9621..9905
                     /locus_tag="KMT45_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004352.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            9978..10457
                     /locus_tag="KMT45_000872"
     CDS             9978..10457
                     /locus_tag="KMT45_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBE7004353.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            10680..10823
                     /locus_tag="KMT45_000873"
     CDS             10680..10823
                     /locus_tag="KMT45_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBE7004354.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            11401..12984
                     /locus_tag="KMT45_000874"
     CDS             11401..12984
                     /locus_tag="KMT45_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBE7004355.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(13421..14605)
                     /locus_tag="KMT45_000875"
     CDS             complement(13421..14605)
                     /locus_tag="KMT45_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBE7004356.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14630..15637)
                     /locus_tag="KMT45_000876"
     CDS             complement(14630..15637)
                     /locus_tag="KMT45_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE7004357.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(15809..16168)
                     /locus_tag="KMT45_000877"
     CDS             complement(15809..16168)
                     /locus_tag="KMT45_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004358.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(16140..16511)
                     /locus_tag="KMT45_000878"
     CDS             complement(16140..16511)
                     /locus_tag="KMT45_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBE7004359.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(16528..16830)
                     /locus_tag="KMT45_000879"
     CDS             complement(16528..16830)
                     /locus_tag="KMT45_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004360.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(16847..17317)
                     /locus_tag="KMT45_000880"
     CDS             complement(16847..17317)
                     /locus_tag="KMT45_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBE7004361.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(17292..17570)
                     /locus_tag="KMT45_000881"
     CDS             complement(17292..17570)
                     /locus_tag="KMT45_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBE7004362.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(17567..18616)
                     /locus_tag="KMT45_000882"
     CDS             complement(17567..18616)
                     /locus_tag="KMT45_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBE7004363.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(18573..19502)
                     /gene="tadA"
                     /locus_tag="KMT45_000883"
     CDS             complement(18573..19502)
                     /gene="tadA"
                     /locus_tag="KMT45_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBE7004364.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            19634..21388
                     /gene="ade"
                     /locus_tag="KMT45_000884"
     CDS             19634..21388
                     /gene="ade"
                     /locus_tag="KMT45_000884"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBE7004365.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(21486..22148)
                     /gene="trmB"
                     /locus_tag="KMT45_000885"
     CDS             complement(21486..22148)
                     /gene="trmB"
                     /locus_tag="KMT45_000885"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBE7004366.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(22218..23000)
                     /locus_tag="KMT45_000886"
     CDS             complement(22218..23000)
                     /locus_tag="KMT45_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBE7004367.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(23204..24415)
                     /locus_tag="KMT45_000887"
     CDS             complement(23204..24415)
                     /locus_tag="KMT45_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004368.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(24408..25145)
                     /locus_tag="KMT45_000888"
     CDS             complement(24408..25145)
                     /locus_tag="KMT45_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004369.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            25283..25717
                     /locus_tag="KMT45_000889"
     CDS             25283..25717
                     /locus_tag="KMT45_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBE7004370.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            25710..26054
                     /locus_tag="KMT45_000890"
     CDS             25710..26054
                     /locus_tag="KMT45_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBE7004371.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            26234..27244
                     /locus_tag="KMT45_000891"
     CDS             26234..27244
                     /locus_tag="KMT45_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE7004372.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(27313..28257)
                     /locus_tag="KMT45_000892"
     CDS             complement(27313..28257)
                     /locus_tag="KMT45_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBE7004373.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(28241..30961)
                     /locus_tag="KMT45_000893"
     CDS             complement(28241..30961)
                     /locus_tag="KMT45_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBE7004374.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(30958..32178)
                     /locus_tag="KMT45_000894"
     CDS             complement(30958..32178)
                     /locus_tag="KMT45_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBE7004375.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(32459..33304)
                     /locus_tag="KMT45_000895"
     CDS             complement(32459..33304)
                     /locus_tag="KMT45_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBE7004376.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         9939 a         6868 c         5816 g        10789 t
ORIGIN      
        1 tttatatcca ctttccggat gaaagcacac caaaaaatga atcagttgct acattgcacg
       61 aattaaaaga agcaggaaaa atccgagcag tcggtgtgtc aaacttcacg ttagaacaat
      121 tgaaagaagc gaatgcagat ggatatgtcg atgtagtaga agataaatac agtttgatcc
      181 atcggcaggc tgaaaaagaa ttattccctt atcttgaaaa aaacaaaatc agttttgttc
      241 cctattttcc tcttgcttcc ggtttgctga caggaaaata tgaacttggt gaggaaaaac
      301 aatttggtga aggagatcct cgaaaaagaa atccagattt ccaaggagaa cgtttcagag
      361 aaattttaac cgctgtcgat gtacttcgcc ccattgctaa aagataccaa gccactcctg
      421 cgcaacttgt tttagcttgg tacatgaaga atccacgagt gtctgtagtt atacctggag
      481 ctaagcgtcc agagcaagta tctgacaatg tccaagcttt agatttgcat ttatccaatg
      541 aagattatca aaccattgat gaagcatttc gtggattttg atcaattgac cttatcaatt
      601 tatagtaata tgaaaaaagg aagatcagca ctgtttgatc ttcctttttt tcgataagtt
      661 tatgctaatc taattcttgt aaagtagcgc tcggctattc ggataagtcg gaaactgatt
      721 gaatggaatc gtccaatctt gttcaggtac agagaactca aacttattta ctagctgatc
      781 ggaaaacaca cgtagactat gaagagtgat ccattctcct gcacagcgat gcatttgtcg
      841 aaaatttccc ccgccttgtg caatcatctc atattcttct ttataggaaa tatcttttgc
      901 tttccctaca taccgtttga tcataaagga atctggcgct tcaactgttc gttcatcatg
      961 gtctgttcca tataaatcta aaataaccca actgccttct ggtatacgat aaccatctac
     1021 ttctacttct ttcagcgaaa tcgcaggtag cattgggaag aaaggataat agcgtctcat
     1081 ttcttgaata aacggatctt gtaacgtcga aaagtcattt tttaattgct gatacaaatc
     1141 tggtctactg aaaagtgcat gtcccatcaa tgccgcccac acagttagag caaccgttgg
     1201 acggatgatg ttcaataatt caacagctgc cacttccaga ggtaaaagct gaccatctaa
     1261 atctgtggca ttggcaaaag catataatgc cacattttct tttccagcca caggattttg
     1321 tcgagcttct tcaatcaagc cttgcgccca tttttctgat tttttgcgat tttcgacacc
     1381 ttttatgtga tcaatcggag aagtaaacgt tccgctgatc atagagattt gatattctgc
     1441 taatttttcg acctcatctt tagaaatcgc tgataaattg atacctgccc actcgcaaat
     1501 agatgtaaag aaaaccatct tacttaaatc aaatagttca aattgaccat gctgtgcttc
     1561 aagtgcttgt gtcagattct tatctaagat acgatgataa tcttccattc tttccggtgt
     1621 cattagatcc ataaaaatat ttttccgatg gtgatgagca gctccatcca aagtctgtac
     1681 tccaccttga ccaaaaagtg tcttcaatac taattttggc atagcacctt ttcgtttgaa
     1741 attccttgga tcataaaata cttttgctgc tgatgatccg tagatcgtga tagcttcttt
     1801 attgaaaatc tttgctttga cgacgggagc gtccgcttca tggcgtaact cctccaacat
     1861 gttgtatcct ttttgataca atttttttaa atctgtgatt ttgatatcta ctactggtac
     1921 ttctttcatc ttcagcaacc tcctataaaa aataacttgt ataatttaag cttaactttt
     1981 tttccgactg ctgacaaaag aagcgctttt tgcagacaaa aaagccactt aagttagagt
     2041 ttatcagtct aacttaagcg acgtgtttca tagtttttac tagtgaaaac cagattactt
     2101 tatttgtttt tagaatcaat aatgatcgtt acaggtccat cattgacgag agaaaccgac
     2161 atatctgctc cgaattgtcc ggtagcgact gtgatgccgc gacttctgat tccttcattg
     2221 aatgattcgt ataaaggaat agctgtttct ggtcgtgcgg cttccacaaa gcttggtcga
     2281 tttccttttt tcgtatccgc aaacagtgtg aattgagaga tacttaaaat ttgtcctccc
     2341 acatcttcta cactaatatt catttttcct tgttcatctt caaaaatacg catttgtgca
     2401 atttttttta ccagatagtc cacatctttt tgcgtatcgg tttcatgaat gcctaataaa
     2461 acaagtaggc cgtagccaat ttttccaatt tcttgctgat cgactgacac agcagcttta
     2521 cttacccgtt gaacaactgc tctcatatct gtcctcctat ccattcgtcc ggcgtacact
     2581 gtagacatca ggtactgctt tgattttatc cacgatactc tttaggtggg ataaattctg
     2641 gataccaacc gtcaaatgga tcgtcgccat tttatcttta ttcgtccgtg cttcaacgct
     2701 gttgagattt ttcgttagcg cattgactgt ttgaagtaca tcattcaata agcctgaacg
     2761 attatagccg taaatttcca aatctgcatc atactctttt cgtgtattcg atgtatcttc
     2821 ccattcgact tcaatcaaac gttctgctac atttggtttg tcaggctgaa cattcggaca
     2881 atctcgacga tgaatggata tccctctgcc tttagtgata taaccaacga tatcatcacc
     2941 aggaatcgga ttacagcagc gactgatacg aattagtaag ttttctaccc cttgaatgac
     3001 aacgccacct tcatgacgta ctttcatttt gtcaggttct tttttcggct gattcatgat
     3061 ttcttctgct tcttgctttt gctgctcgat tttttgttct ttacgttctt tttcagtcag
     3121 acgattggcc atcgtcaagg ggctaacttc tccataccct acagctgcat agagatcatc
     3181 ttctgtttga taattaaaac gatcgagtgc ttcttgcagt ttgttcttcg tcaaaatatc
     3241 tttaggcgta aatcctagat ctgtaataca tttgaccacg gattcgtggc ctttgatcac
     3301 attttcttct cgatcttggg ctttgaagaa acgtttgatc ttatttcttg ctttgctagt
     3361 agcaactaat ttcaaccagt cgcgacttgg gccaaatgaa tttggagaag tcatgatctc
     3421 aatgatatct ccgtttttca atttgtaatc aagctgcacc attttgccat ttacttttgc
     3481 accagtggtt ttgttaccga tatctgtatg gatactgtat gcaaagtcca atggtccgga
     3541 tccttttggt aactcagtaa cgtctccttt tggtgtgaag acgtagactt tatcactaaa
     3601 gatatctcct tttacgcctt ccataaattc agaagcatca tagctttcgt cttgaagttc
     3661 gagtatctca tggaaccagc ttaattgttt cgtcatacca tctggttcta ctttttcatt
     3721 ttttccttct ttataggccc agtgtgcagc aaccccgaat tcagcgattt catgcatttc
     3781 ctgtgttcgg atttgaattt ctaccggatt tcctgccgga ccgatcacgg tagtatgcaa
     3841 agattggtac atattagctt ttggcatggc aatataatcc ttaaatctgc cgggcattgg
     3901 tttccatttt gtgtggattg ctcctaatac agcataacaa tccttgattg aatctacgat
     3961 cactcgaata gctagcaagt cataaatttc gttgaactgc tttttctgat ctttcatttt
     4021 acgatagatt gaataaatgt gttttggccg tccatagatt tctgcaaaaa tccccagctc
     4081 ttctgtcgct attcgaatat cttcaacagt accacttacg tatttttcac gttcttctct
     4141 cttcgtctgc atcaagtgga cgatacggta atactgtttt ggatttagat aacgaagtgc
     4201 tgtatcttct aattcccatt tgatccggct gatccctaga cgatgtgcaa gaggcgcata
     4261 gatttctaaa gtttcctgag cgatccttct ttgcttatct tctcgcaggt gcttcaacgt
     4321 acgcatgtta tgtagtctat cagctaattt gaccatgata actcgtaaat cttgagccat
     4381 tgctagcagc atctttcggt ggttttctgc tagctgctct tcgtgggatt tatattttat
     4441 tttccctaat ttggttacgc cgtccactaa catagcaacg tcatcgccaa attcttctct
     4501 cagatcttct aaagtgattt ctgtatcttc aacaacatca tgtaagaagc ctgtagccac
     4561 tgtatgggga tccattttta aatctgctaa tatacctgcg acttggatag gatgaataat
     4621 gtagggttcg cctgattttc taaactgtcc gtcatgtgca gcagtcgcgt attcgcatgc
     4681 tttctcaaca aatgctacat gttcgggacc catgtattga ctaacgagct ttatgacacc
     4741 gggtcccgtt aaaatttctt cttttgtcac aggaatcgat ccccctttct tttttcggta
     4801 aaacgggaat cgggacaaat atctgtttga taattgtccc aaaatccgtg cagttatacg
     4861 tttttttacg gaaatgttcg ctttttaaaa gctttttcaa acgatttttc gtatccattg
     4921 tataaataat tgtttctatt atacaaattg ttgactattt tttcaattct ttctgtcgat
     4981 actcttttat taatgaagaa cgttagctga aagaaggatt cattaattcc cattgatagc
     5041 tagcagcact taataaataa agaggtgctg tttctgtacg aaggattctt ggtcctaatc
     5101 cacaaagtat ccctttgtgt tctacaaaat aatcgacttc atcgcgggat aatccacctt
     5161 ctggtccaaa gacagctaag attcgactat ctggttttgt ttcactaaga attttggctg
     5221 catttgattt ttcgccttgc tttgctgatt cctcataagc aaccaagacc acgtcataat
     5281 catctagccg ctcaaataaa tcttgtttcg tttccaataa ctctactgat ggcacgatct
     5341 gccgatggga ttgttccgct gcctcttgag caatcttttc aagccgttgt tgtcgtttca
     5401 tacgtttttt tgcatcccat ttaacgacag aggctgatgc cgggaaacca agaaaagctt
     5461 gcgctcctaa ttcagtggat ttctgaacga tccaatccag cttttcccct ttaggatagc
     5521 cactcgctat ggtaatcgaa cttggcagtt ccttttgatt ttcttctttt ttaatttctt
     5581 tcagataaac agcctcttca gtaatctccg tgatttctgc aacaatggct atttgatcat
     5641 tgaatgccaa aaaaacttca tcctcgggtt ccatcctcat aacccgtaca atgtggtgat
     5701 agttttcccc ttctaaaaca aaatagtctt ttgattcata tgattctttt aaaaaatacc
     5761 gctgcatcta ttccacctct ggttttttca aaataattgc ataccagtct ttttgctgga
     5821 aaatttgatc cacgataaac ccttgttcta acatggcatc cagaaccatt tgcttcttct
     5881 cttcaatgat tcctgaaacg atcagcgtgc ctgtttgctt cagtaaacgc caagcatctg
     5941 ggatcatcaa taagatgata tctgctaaaa tgttcgcaac gattacatca ctttcaatct
     6001 cgattcctgt caaaagatcg ttagctgata catggacatc tttggctatt tcattcatat
     6061 ccatattttc tttcgccgaa cgaacggcta cttcgtctaa atcaaaggca tagacatctt
     6121 ttgcaccaag atattttgct gcaatactta gcacgcccga cccagttccc acatctagca
     6181 atgtttcacc accacgcaag actgtttcta gtgctgtcaa tgtcaaggaa gtcgtgggat
     6241 gagtgcctgt gccaaatgcc ataccaggat ctagtgtgat aattttttct agaggatctt
     6301 ctgtctggta gttttcccag cttggcgcaa ccgtcagcat tcgcgtaata cgcaccggat
     6361 gatagtattt cttccaggct gttgcccagt tgctttcctc tacttcacta actgttactt
     6421 tattttttcc aatagctaga ccaaactcag gtaattgtgt gatacgatct ttgataaaag
     6481 gcaagatttc cggtaagaaa gtcgtttccg gaaaataagc catgactagt gcgccttctt
     6541 tcagcgatgt aaaatcattt ttatctaaaa tctcaccata ttgatccgac tgaaaatgtt
     6601 caacatctaa agaatcttca atcgcgacac cactcgcccc tgcctccatc aaaatattag
     6661 caactgcttc gactgcttca ctttcagttt ctacttttac ttcattccat ttcatatctg
     6721 tcactcctta ataacttggg tacggtaaat aagtatctgc ttcattttgc tgttttactt
     6781 ctactaattc tgctagttgc tgattatccc agtgaagctc gaatacagct tcgtcatcgt
     6841 cttcatccaa aaaatcaagc aaatcgcttt gcccttgagc taatacttca tttagatatt
     6901 ccactaaaga atcagctact gattttgcca accctttttt tccttcataa gggattactg
     6961 ctagataatt gcgttcatca aaaacagatt tctgaggatt aaaaagaagt acgccgtctt
     7021 caaattcgat aattggttct tgcgaaacga tcccttctgc atcatcaatc tcaaattgtg
     7081 ctttattttc tgcaaacaag cgaacaacga tctcgatcgt atgatttttt gtatcccaat
     7141 ctaaagctac atcatagtct gtcacttgtt tttcaattat ttcatctaaa tatgttaata
     7201 gatccttctt tttcattttc tttcctccaa ccccaatctt tctcgatttg gttttttctt
     7261 tgtaaagaga tataaggatt tcccgacaca acaaaacgca acggcttttc tgtccataca
     7321 cctttgtttg ggataccgat tctaggcaaa gcattgatct tttttggtgt cttttttttc
     7381 tcaaaaacta agcgtaacga actatctgct atagactgac cataaagttc ttgaggaatt
     7441 gctaatgctt ccactaattt tcccggaccg ttgctgatat ctggaccagt ttttccgcca
     7501 cgatttttac tcatttgatc aatcattgcg gcgggttcga ttgcacgtat cattactcct
     7561 tgaggaatcc cttctggctg agtaatgatg ttcaaaatcc gatgagtatg catcgtgtaa
     7621 aggtagatcg tacctggttt ttcatacatt gcccgcacac gcggtgttct tcttaaacca
     7681 tagctatggg cggcctcgtc ttctggacct aggtatgctt ccgcgtctac aatatatcca
     7741 gctaatcttc cttctggtgt gtcatgctcc aaatacatcc ccaataaatc ccgtgccact
     7801 tctggcgtcg ttttattcca aaaaaattca tctatctttt gtttatccgt catttttctc
     7861 acctacttta tcatacacaa tattagttgg ataagctatg aaaacaaaac aacagaaggc
     7921 tgttctttct aggcatcttg ctaaatatga tataattaag ggacgtaaaa acaagaagga
     7981 ggaaaatcct atgcaacagc cattggctta tcgcatgaga cctcgaacga tcgatgaagt
     8041 cgttggtcag cagcatctag tcggggaagg aaaaatcata cgccgcatgg tcgatgctcg
     8101 catgctctct tccatgattc tatatggacc tcctggtact ggaaaaacga gtatcgcaag
     8161 tgcgatcgcc ggctctacta attatgcatt tcgcatgcta aacgccgcta cagattcaaa
     8221 aaaggatcta caagttgtag cggaagaagc aaaaatgagt ggtacagtga tcctcttatt
     8281 ggatgaagta caccgattag ataaaacaaa acaggatttt ctgcttcctc atttagaaag
     8341 tggccgaatc atcttgatcg gagcgactac tgagaatcct tatattacca tcaatccagc
     8401 gatccgcagc cgtacacaga tctttgaagt caaaccatta acagaacaag acatccagtt
     8461 ggcagtcgaa catgctttaa gggataaaga acgcggcctt ggacaacaag caatccagtt
     8521 agacgaagat gctcttcttc acctttcgcg tgcaacgaac ggcgatttgc ggagtgcatt
     8581 gaatgggctg gaactggcta cactatcgac tccttcagat aaagaaggaa ggattcatct
     8641 tacactgtcg atcattgaag aatgtgtcca aagaaaagct cttacccatg acaaaaatgg
     8701 agatgctcat tatgacgtta tttcggcttt tcaaaaatcg attcgtggaa gtgatgtgga
     8761 tgctgcactt cattatctcg cccgtttagt tgaggcaggt gatctggcaa gtatttgccg
     8821 gcgtctgatg gttatcggtt atgaagatat aggtttaggt aatcctgctg ctgcggctcg
     8881 tacagtgaac gctgtattag ctgctgaaaa attgggattg cctgaggcac gtatcccact
     8941 agcagacgtc gtcgtcgatc tttgcttgtc gccaaaatcc aattctgcct acatggcatt
     9001 agatgcagca cttgctgata ttcgagaggg caaagctgga gatgttcctg atcatttgag
     9061 agatagccat tacaaaggtg cgaaatcact caaccgtggc gtgggttatc agtatcctca
     9121 tcattttgat caggcttggg tcaatcagca atatttacca gataaactga agaatgctca
     9181 gtattatcag ccaaaagata cagggaaata tgaacaggct ctgggtcaac agtattaccg
     9241 tataaaagag tggaaaaagc atcctccaaa aaactagaca agaaaacggt tctgtttctg
     9301 cgagctctct cattgctctt tttaaattca tatgctacta tagaggtggg aaatctgtga
     9361 tgttcgaatt gtcctctttg tgtgttgacc gaacattttt attgatatct tgggacccgt
     9421 cttgtgtcac tgctggtaac atgccttatc atttggtagt tcgaagctac gaccatggga
     9481 cccacctgct tgaattcgcg ggatcaatac tacaggacaa acaacggcat taacggagat
     9541 taccatcttt taaagaataa agcaatttgc tcttggcaaa ttgctttttc tatttgttgc
     9601 ttattttgga ggatttgttc atgattagga ttttaatgat tatcgccact ttagtattat
     9661 tattcgttag ctattattta tttaataaac aggatatctt ttttgtattg atcaacaaaa
     9721 atgataaaaa tcaaggattc ctacaatttt acggagctgc atatgctgtt ttaggcgtaa
     9781 tgggtatttt aacagctttt ttcaatcaac gctttattgc cttggtcttt ttactgatcg
     9841 taattattgt ttctgccacc ttcagtatcc gatttgcaaa aaaaattgct gaaccaaagc
     9901 agtaaataat tctctttttc cctaaagtgt tctacaataa aagagtacaa tacgattgac
     9961 ttgaggagtg aatgcttatg ttacaacaat acaaaaaaat catggttgcc gtagatggtt
    10021 ctgatgaagc tgaattagct tttaaaaagg cggtcaatgt agctatccga aataacggag
    10081 aacttctatt agcacatgtc attgacacac gttctttcca aaccgtttca tcctttgatg
    10141 gaatgcttgc agaacaagct actgaaatgg caaagcaaac attggcagat tatgaaagca
    10201 atgcgaagaa agctggcttg aataatgtaa cctctgtcgt agaatatggc tcaccaaaac
    10261 aaattattgc cagagaaatc ccagaagata atcaagtaga tttgattatg ttaggagcca
    10321 caggattgaa tgctgtagaa cgtttattta ttggttctgt ttctgaatat gttattagaa
    10381 acgcagcttg cgatgtgctt gttgtccgaa ctgatttaga aaaccaattg ccagctaaag
    10441 accacaccaa agcttaacac gtagacgacc tgccaactat cggcaggttt ttttgcacaa
    10501 aaaaccgtga taaaaacaaa agacgcggca agcactcatc ggtctttata atgtttaaaa
    10561 taaagggaaa acagtcaatg agttcaagta aaataaaaaa aagtgaacac cagggcgatt
    10621 agtcgccctg gtgtggttta aaacgaccta agcatgccaa agcttattgg ttcgtcaatg
    10681 tggaagtttc cgatgaatct gatacgaaaa aatatgaagt atcgatggcg gataccgatt
    10741 ttatcgccaa actaaaattt tatttcgggt tcgggtaccg cgagaaggca agtgctacat
    10801 tcggtaaatc attcaagaat tgatgaaagg ctaccgcttg ccattgcaac cacaacgata
    10861 ctcctgaacg cgatgttggt gagatttcca aagagaagag ctgtttaatg caacggtatt
    10921 aagcaaatgg caaaaggaag tcatgtagac aaagtttttt atcaaacacc atatgatttc
    10981 gcctgaacgc tggtttagct cagaatacaa tgatgtggtc cacgagacat taccctcagt
    11041 cagataaaaa aagctctctg acagaattac acatcgataa atattaagta aatgtcagcg
    11101 ctagttgaac tttctgacac tttcaggtac actggaaaag acaactacac gaaggaggga
    11161 aaatactatg atgcgcatga ctaaaataac aagtaatgaa aattaccaac gataccatat
    11221 cgcatttagc catgcaattg gttttacagt tgctgttgac gggacaagtt tgtccaaaac
    11281 agttgaattc acgtaaaatc aatggccatc tcaaggtatt tttctcatga taatctgagt
    11341 ttaaataaca caggccatgg ataaccatgg cctttttgag tggtctcaag gagagagatc
    11401 atgatcatac aagcaatttc tattaaaaaa acaatcggcg ggaatccatt atttgaggat
    11461 ttatcctgtc aaattgaagc tggtgaaaaa attgccttag tcggtatcaa cggaactggc
    11521 aaatcaacct ttttacaaat cttgaccggc agagaaggtg tcgacagcgg tgttatcagt
    11581 cgcaaaaaag gcttaaagat cgggacggtc gaacaagaac ttacagtttc cgaagcgacc
    11641 gtcaaccact acttgctcca ttctgccgct gagatacagg acctcaaaca gcaaatgagt
    11701 cgctacgaag cattgatgac agaaccagat acaaacttag agaagtgtct ggcccgttac
    11761 ggtgagctgc agcatcgctt tgaagaacta ggcggttacg tattagaaga ccggattgca
    11821 gcgatcttgc aaggcttagg gattccacac cgtcaggaag cacggttaac agaacttagc
    11881 ggaggcgaac gagttaaagt cgctttagcc aaaattttag cagccgatgc cgatttactt
    11941 ttactcgatg aaccgaccaa tcatttagat cttaacagta ttcgctggtt ggaaaactat
    12001 ttacagaata caaaaaaatc ggttctggtc gtttctcatg atcggcaatt tctcgatcaa
    12061 gtcaccacaa aaacatggga actggaagaa ggtgcattga ttgagtatcc cggaaactac
    12121 agccgttttc gagtcttgaa agaagctcgc ttggctgagc tgacaaaaaa ttacgaactg
    12181 cagcaaaaag aagtccagcg gttgaaagtc atgattcgtc gttttcgtca gtgggcacat
    12241 gaaggagaca atgagtcctt cttcaaaaaa gccaaagaac tggaacgccg cctggcgaaa
    12301 ctgacactcg tcaaaccgcc acctcccccg aaaaatcggc tgcaaagctt gtctaatggt
    12361 ggaaaatctg gcaaagaagt tttcatcatt caaaacttgc accagcagta cgctgatcaa
    12421 gtactattca aagacagcag ttttgcagtc tatcgtggag atcatttagc catcatcggc
    12481 gacaatggtg caggaaaatc aaccttactc aaattattac tcggtgagga aaagccttac
    12541 ggtggcacca tcaaattagg tagtagcttg caaatcggtt atttgccgca gcagctgcag
    12601 tttgccgatc ccgatagccg tttgctggct tacgccatca cattgactgg aaacgaagaa
    12661 gtcgctcgac ggcaattagc caaaagtggc ttttatcaaa gcgatgtggg taaacggatc
    12721 aaagatttat ccggtggtga aaaaatccgt ttggcgttgc tgaaactctt tttagaaaaa
    12781 atcaacgtcc tgattttgga tgaaccaacc aatcacctcg acagctatgc tcgagaagaa
    12841 attgaagaaa tgctgcaaga ctatcaagga acgctcctag cagtcagcca tgaccgctac
    12901 tttttgcagc aacacttcca agaagcactc gttattgatc aggagcagat cagccgccag
    12961 ccgctcggcc aactgattga ctaggacgtt ttttcgcaac aaaaaacttg attttcatac
    13021 ctgtgctttc acgattcagg caaagtacag gtggaaaatc aagtttttat tattcgatca
    13081 atgaatgttt cggatcggtt ttctagctaa acataaagcg aagaataaag tcgacagccc
    13141 acgaggctga tctaaaaatg ttttttcttt ttacatatgt aatgtccctt ataaagatga
    13201 atgaaaataa aatattggga ttttcctgaa aatcaataaa taaaatgcca gtcttttccc
    13261 ttttgaccac ttttccaagc aaaattccct aagaagatcc tgtaaaaaag aagggaaatt
    13321 gaagttaaat taccattttg cggtcttctc cgcaaaaaaa acacatgcaa gaattccctc
    13381 ttgcatgcgt ttcatctttt atcctaacga atgctttggc ttagccgcgc agacgctcca
    13441 catcacgggc aatcattagt tcctcatctg ttgggatcac taatactctt acttttgatt
    13501 cttttgtaga gatatctact tcttcaccac gcaattcatt caacgctgga tcgatttcac
    13561 aaccaaacca agacatacct ttgatgattt cactacgtac atgtccgtca ttctcgccga
    13621 taccagcagt aaagacaatt gcatcaacac cattcatcgt tgtcacataa ccgccgatgt
    13681 attttcggat gcggtccacg aaaatgtcat aagctaaacg aatatgttct ttttcatagt
    13741 tcttttcaag atcacgcata tcactagaca atcctgttag gcctagcaga ccagatttct
    13801 tgttcaaaac attgatcatt tcatcaatat caatctctaa tttttccatt aaatatggga
    13861 gaacagctgg atcgatatca cctgaacgtg tacccattgt caccccagct aatggtgtga
    13921 atcccataga agtatccatg gatttacctc catcgactgc tgtgatcgat acaccatttc
    13981 ctaagtggca agtgatgatt tttagttctt cgatcggttt gttcaataat tttgccgcac
    14041 gttccgacac ataacgatga cttgtgccat ggaagccata ttttcttact ttgaaatctt
    14101 cataataaga caccggtaag ctatataaat aattttgctt tggcattgtt gagtggaaag
    14161 atgtatcgaa tacagccaca ctcaaaatgt caggtaaaat ctttttaaac gctttgatcc
    14221 ccattaagtt tgcaggatta tgcaaaggag caaattctgc taattcagca atgcggtcca
    14281 ttactttctc atcgatcact actgaatccc cataagtttc tcctccgtga acaacacggt
    14341 gccctactcc ggtgatttca tcataagagc ttaaaatttg cagttcaatc aactgatcta
    14401 acagcatttt tactgctact tcgtggtttt cgatatccat gatttgttcg aatttttgat
    14461 tatcgccgta cttgatggta aaaatagaat cttgtaaacc gatacgttcg acgatccctt
    14521 tcgcaactac ggtttcttct ggcatttgat ataattgcca tttcaaactt gaactcccag
    14581 cattgattgc aattgtttta gacatatgac tctccttctc gttgaatgat tacagagcag
    14641 atgatttcca gtttttaaac tcgttgaaaa attccgtcac cttctctggt tcttttaatg
    14701 aagcaagttt cacaagcaag acttctttta cttgttgtgc ttgtggtcct tttttctgta
    14761 agatcaaaat acttttttgc gattgtttat tacggaacaa ttcatctggc aattgaatca
    14821 ttccttgaag ataaccttct tctttaaacc attttttgat ttcttcactt tgatccgttt
    14881 ctaaaaatcc actcggcatc aaaaacaaac caaaaccatc cggcttcaca tatttcattg
    14941 attgctctag caataaatga tgtgcataac tatggccttc ttcggtactt gttaaaaact
    15001 cttttgcttt ttcgtcattc ggataataac cgattggcaa atcgctgatt gcaaaatcga
    15061 caggatctat taataattcc tgcaacccgt cttgatgaaa gtattgaaca tttgcttgtg
    15121 tcaaatcact tgtagaagct gcgacagcca acaatgtatc atcaatatcc acaccaattc
    15181 cttctgtttg gtaccccgca ttcgataggt ttaaaagaac tgttaataac aagttgccca
    15241 ttcctgccgc aatatccaga atcttaaccg gtgctttttt atttgtgtat agttgttcaa
    15301 taagaaaaac aaataaaaaa ccgatactat caggagtgag ttgatggttc gcctgcaaat
    15361 gttctgtctg gctgcccttc aataaaagta attgagacag tctgcgccat tcttctggct
    15421 cgaaagaaag tttcttcaac tcttcataaa gagctgtgat ccgttgcgtt gtctcttttg
    15481 tcggcactcc atccaccaca cgtacttgat aatcatctat taaattttct gcattttcga
    15541 cataggcttc taaaaaggaa gttcccaatg cattctgtaa aagctgaata gcttccaggt
    15601 tttgattaaa cccttgttcc attttttctg gtaacatggt gcacctcatt tctagatata
    15661 ttataacaat tttttcacct gtttaattct atcgaacaat tgacaaaatt tcaatcgttt
    15721 tcactgtttt ttatttgtga aacggcaaac atttttttat taaaaacagt gttataacag
    15781 aaaagagggc gttttcacga acattccatc attctgtact agtatctgta tcatttttta
    15841 cttcttctgt gaaccgaaag ataaacggtg agacttccac ctctaagaat agggactgtt
    15901 gtacccactg atatgttaca gatccagttg tgtagtaaaa aactccttcc gaactttcgg
    15961 caagcgtaga ctgttccgat aaagccatct gcttcattat ttttgctata taataatttt
    16021 tgattcgtat attaagatcc attgacaaac ggtagctttc taatgctgcc atcaataaaa
    16081 acgtacataa aagaaataaa agcaagacag aaaataaaat agagccctta taacattttc
    16141 tactgatcgt tttttgtcca tatggcatat ccatcttctc cattttcaaa gtgtacataa
    16201 aaagaaattg tgtgttcttt ttccaataat tgaagctcag tgatacttgt taacataggt
    16261 tgatgtccgc cggaagttct tcttttacgt atcattgcct gatatctttc gatgatatac
    16321 tgtttatcct cgatcgtgta gtgcagttgc tgatttttca cagagacatt gtgactgctc
    16381 ttgagttctt cttctaattg tgctaaaaat attagccact ctttttgatc tttacgctgc
    16441 aaaaattcac ttacttggat ggtttgctga atcaaaagat tgaatgtgag caatagactg
    16501 ctcaatatca tcagtccaat tagacattca atcagcgtaa atgctggaat tacttgtttt
    16561 ttcaatactg atcgcttctt tctccatatt gacaaacact ttttcgatta ctccattatt
    16621 atcgacaatt tggatatcgt atgggatcat ttcttccctt cgctctggtc ttttgtgata
    16681 catccgctgc gtcttgatat cttcataaat cattcgaata aatagcagtt cctccccact
    16741 tttcttttct gcctgaagca aaaacagctg gccaaagctc actaagtaga cacagacaga
    16801 agcgatcata agcgctgtaa gggactccaa caaaacaaat cctgttttat tcaattcttt
    16861 ttgtatagcg accactccct atctggaatt gatacgtaat cgtccgctct ttttcttccc
    16921 aataaaattg ataagctttt agtgaacttt cattcccggt tctagcagca aaagtaatag
    16981 aagcatggcg tttcaaggta atctcatcag gaatatctaa tacttcccaa tttattttat
    17041 ctggattcgg aacatgaaaa atgatttgat tctcttcact attccaataa aagcctgttt
    17101 ggatctgatt cacaatagcg attttttgcg tagaatagat tcgtttttca aattggccga
    17161 aaaattgata gactgccaat tcattcttcc atgcagaaaa tgcgagaact ggaaaagaaa
    17221 ggatcgttgt aagtaataaa atcagtaaga gtgattcaaa caacgtatat cctgatgcta
    17281 aaaccttacc ttcaaggccg ctgatagatt tctgcatgtt ctcgtttgat atagccttca
    17341 ttcaacaatt cctccacagt aggaactctt ccattatttt gaagcgcata gagctctttt
    17401 tgtgtgtcta ccaactggat gatcgcttca cgactttctt ggtctacatg attcctataa
    17461 cgcgacaaat tagggacgaa caacaacact aatacactga tgatcagtaa gacgattagc
    17521 atttcgatca atgtaaaacc gctataataa gatatttttc tctttttcat ggcaaaacct
    17581 cctctatgct tccgtaaaca ggtaataaaa cagcggcata aactaataaa atcaaagccg
    17641 ctacgcccaa aaaaatgatt ggctgaataa aatgcatcca gtgatgaaga cgtttgaaaa
    17701 gctgttttct tgtccattcg ctgtaaaaaa tcagttcttt acctaaactt ccctttgcct
    17761 ctccctgtaa aacaatttta ctgaattctt ctgatagaaa acgataattc tggaactgct
    17821 cagccaatga catacctgcc tccaatcctt gaatcaactt agaggcaaga tgctgaatca
    17881 gactttcttg tttcgtttcc tttaagcaga ctatgatttg ccttaattcg aaaccttcat
    17941 gaaaaagctt tccaatctct agtgacaaat aagccgattg atacaaggaa tatatcggtc
    18001 caatcaataa ttgtctgctt atccattcac tctgatcaac aggagacaac ttcatcagac
    18061 gataccttac tagtaaagcc aatagagtaa ccgctaggat gaatagtaga acataccaat
    18121 gaaagacttg tatcagctga ataccccaat gctcttgcat gaccattcct gaaccaataa
    18181 gctgaggcaa aacgaacaac cgcatactaa acaagatacc tagcaaaaag gtgagcaata
    18241 gcatcggata tgcaagcagc tttttcaaat cattgcgaaa tccttcaaca agccgaaact
    18301 gtttggcaat ttccaacaaa gttgtcggca gattcccatg acgttcagct aactcgatct
    18361 gtaccagttg ctctgctcca tatcctattt gcgaaagtac ctcactaaac gcttgccctt
    18421 cctttagctg atgttggata tttgccaaaa taacaggtgg aaacgaacgg atcgtgagta
    18481 ataaataaag ggattcttgc agactaaaac cagataaaag cagttctccc atggtttgtg
    18541 caaactggtt ttgctggctt cttgttaatc ctttagaata gttctgactg ccaaatattt
    18601 tcctgagctt cttcataact ttcctcccat gattttttta ctgattcttc ctccataaat
    18661 tgataactcc ataaaacaga aggatggtga ttggcatctt ccaaaagttc ttgataaatg
    18721 acaccagcta aacattcaga tagctcttct tttttctttg tcagttcatg gattctgacc
    18781 aatgtacctt caagattgcg tgcatgtacg gttgcaaaaa cccggtgccc tgttagtgct
    18841 gctcttaccg cagcatgtgc agttgattca tctcttatct cgccaatgat tagcaagtct
    18901 ggacgatgcc gtaatgcgag tttcaaaagt gtatcataag tctgtgctat tttcggattg
    18961 atctgtagct gcagaaaaaa cggttcttct atctctacag gatcttcgat agtaatcact
    19021 tgcaattttt cttccaaagc caaccgatac attagagaag tcttgcctga tccgactggt
    19081 ccgctgaaaa ggtataatcc acgtctgacg gttttctgta aaatagttgc aaaatctcga
    19141 gaactaaagc aacgatgggg tgtttcaaaa gtaccgtata atagccgaat cactaaactc
    19201 tctcgttgaa ggtaatctcc tacagaagac aaccgcagac gaacttcctg tttttctaaa
    19261 gaataagtaa atgcacctaa ctgagctttt cttttttctc cgatatccat ctgtcccaaa
    19321 tacttaaatc ttgagatcag ttgctgtcca tctgccgaag agactttctt ccagaatgtt
    19381 cgtttttttc catagcgaaa atagatgtaa aatacttctg aagctggcaa tatatatacg
    19441 tcttgggcat gtttggcaaa actttcactt atcatttgat ccgataatcg ttttatttcc
    19501 attattctcc cctccacaaa aaacatacgt ctttttgatc tttttgtttc aaataaaatg
    19561 aatttacaaa aaaatattgg caaaagccga atttatgtta tacttataaa aatttatctt
    19621 gggaggcact ccattggaaa caacattaag gaaatatatc gaagtagcaa gtaaaaaaaa
    19681 gcctgctgac ctagtgatca agcatgctaa gatcgtcaac gtctttacga aagaaatcat
    19741 ggaaaaagat gtagcaatct gcgaaggaat gatcgtagga atcggtgaat acgaaggaaa
    19801 aagcgtacat aatgctaatg ggcaatatct agtgccagga ttcatcgatg gccatgtgca
    19861 tatcgaaagc tcacttttat caccaagcga attttcaaaa gtatccttgc ttcatggagt
    19921 tactactgta gtgacagatc cccatgaaat aggaaacatt gctgggagta caggtcttga
    19981 gtttatgata gaagatgcaa gacaaacact catgaatatc ttttttatgc taccttcttg
    20041 tgtacctgta accccttttg agaccaatgg tgcaaattta aatgcaaaaa aattagcccc
    20101 ttttttacaa aaacccgaag ttttaggctt agctgaagtc atgaactatc aagcagtagc
    20161 aaataatgaa acagacatcc tagaaaagat ccaattgatg caccagcata agaaaaaaat
    20221 tgacgggcat gcagctggta tcggcatgga agagttaaat gtttatcctg cagcaggtat
    20281 tcggacggat catgaagcta caactgcaaa agaagctaaa gaacgtttgg atcttggtat
    20341 gtatttaatg gttcgagaag ggaccgttgc aaaagatctg gcatcgctac taccggcgat
    20401 cacacctgaa aattcaagaa gatgcttctt tgtcaccgat gataaactga tcaatgattt
    20461 agtaaaagaa ggatcaatcg atcatatcgt ccgtcaagct attgcacttg gaatcgatcc
    20521 attacaagcc attcagatgg caacattgaa tgcagcagaa tgttttgatt tgaaaacact
    20581 aggtgcgatt gcccctgggt atcaagctga tttcttctta acagacgatc ttaccaagct
    20641 gccaattcat gaagttttct ataagggaac attagttgtt caatctgggc aactccaaga
    20701 ccatttattt gctgatcatt caaattcata tacgtgttgt ctgccaaaac taaacgctca
    20761 accattaaat ccacattcgt tagagctgct tctttcttct tcacaagccc atgtgatcga
    20821 aattatcccg aatagtctag tgactaaaga tttggtagaa aaagtggacc gacaaggcga
    20881 acgattcatt ccttcaatcg aaaaagacca attaaagatt gcggtgatcg agcgtcacca
    20941 tcaaacggga aacattggat taggcatagt gaaaggattt cagctgaaac aaggagcgat
    21001 agcgacaacg attgcccatg actcgcataa tctagttgtc gttgggacaa acgacgagga
    21061 tatgctgtat gctgctaatc ttttgattca aaaaggtggc gggatgaccg ttgtcaacaa
    21121 acagcaggaa atagcttgtg tttctcttcc tataggcgga atcatgacac aggaaccttt
    21181 ttcgatcgtc tatgaacagt tgaatgaatt aataactaaa gcacatcaac taggagcagc
    21241 taaaaccttt gatccttttt tgacactttc tttcttaaca ttatctgtca tacccgaatt
    21301 gaagatcacc gacaaaggat tattttcatt ttctcgcttc caattgattc caccttgtgg
    21361 aaaaaccgat gaaataaata cattttaaat caaataaatc ccagaaaata cattacatct
    21421 gtaatgttca ttttctgaga tttatttatg gatattaatg tttttatgaa agcagaaagt
    21481 tagtttcaca gacttttctt tttggtctta tcttgaaatt gagcttctac tcgataaata
    21541 cgttgacctt ttgaagaaaa tttttcttca tactccgtca taatattgcc ttcaaattga
    21601 ctttgatgta aatctaacca tacctgtttg aggatcatgc catattgaga aaaacttgcc
    21661 agagaatact caaacaatcc ttgattatct gttttgaaat ggatttctcc atttggacga
    21721 agaatctgtt catctacagc aagaaaagtc ttatatgtca atcgccgttt ttcatgacgt
    21781 ttttttggcc atggatcgga aaaattcaga tagatttgat ctacttcgct gtcagcgaaa
    21841 tactctgtca acgctgatcc atccacatgt agaagtttca ggttaggaag cggttgttcg
    21901 atcaacttgt ctagtgcaat cgaaacaaca ctgacctgca tttctactcc aatataattg
    21961 atttcaggat gggcttttgc cattccagtg atgaactggc ctttccccat accaatctca
    22021 atgtggattg gatgatcgtt ttcaaacaat tcattccatt ttcccttcca caatgttgga
    22081 tcactaatta caaaattcgg gtgagccgct aacatttccg ctgctcccgg acgatttcgg
    22141 acacgcatta tttatctcca ttcctttcct ctaattaaaa aggtctggcc aaatctccgc
    22201 agagtttggg ccaaacctca gccactgaaa attcgtttta attgtaaaat ttcttcgttc
    22261 atccgtcgat gatcttcacg tttgcaatat ctggtgattt cttggagcaa attcatggcc
    22321 gcataccaat gaaatttttc aagcatttcg tcagtcgggc gtatgccata actgatcagc
    22381 cactgattcc agctagacag tggcacataa tttcctaaaa tcgttgcaat atccaccgca
    22441 gggtctgcaa acatgattga atcccaatcg actagatata aataattttt acatactaac
    22501 caattacgat ggtttacatc tccgtgtact actgataagt tttccttatc tattgacggg
    22561 atctgctcac gaagaaaacg atggacaatt tgcagaaatt gatttttctg gagttcttcc
    22621 ggtaattctt tttcatattt ttgcagcatc ttctccggcg tcatgacacg accaccgata
    22681 cgggtcagca tggttttaag agaatttgaa tgatgtaaat ggtacaaaac atcaatcacg
    22741 tcgtttcttt gaccaatctc attaggagtc agcaagcgac cttccagcca ctcttgtgct
    22801 gtcaaagtat cgccattccc cgttcgtttt gtccaaacaa gttttggtgt aattccttcc
    22861 cttgaaagag cagccaacat cggagtcgtg tttcgtttaa taaagacttt gtcctcatca
    22921 cgcatgccta tgtatgtttt tcctgtgtcg cctttgatgg gacgtaaccg ccactcttgg
    22981 tctaactgaa actccatcat gcattctccc gtcctcttac tctttgtttt gcagtactta
    23041 ttattctagt cgcctgaaca ctacacgtca agcacatata cgttcttttt catttaattg
    23101 ccattcaaaa aaccagcttt tctcaaaaaa aatagaacga acagcattct tactgtcaac
    23161 gctctatttt tcattctcat ttttacccta cacagctttg ttttcagtct gacatcttct
    23221 tcaaacgagt tggtacatat agtttaacaa agagcatagt aacaatcaaa taagccgcta
    23281 gcggaaccca tcgctgctgc ccatctaata cgactgcagc aatcaaacca aaaataaccg
    23341 acgcgataat taatacccat gtgagtagat actgaatcgc ctgcttcttc tgattcttat
    23401 ggattggata aagctgaacg agaaccatat agcggaactg gttcgctaaa ggtaacagtt
    23461 ggaaaccgat caaatataaa aataagaaac cgataatcag actaaaataa aagtcgacta
    23521 ctccggcaat caataaccca cctataatta ctaatcggaa ataaagacca ctaaaatcag
    23581 aacctcttgc caatcttctt gcaaacaagt aaagataagt gttttttgat tctcttttaa
    23641 tatgagtcaa tataggatcc aaatagcgtc ttcttttgat ctttgccgta atttcaggaa
    23701 catctgtgaa taaattgata aattgataga tctgatgcaa cctatgttct tctacatgca
    23761 ccattttctc ccaatccaat cctttttctt gttgcttcca caaaaaccag taatatagac
    23821 ttgcctgtac taatgcacct gctattcctg cccatattag ccaataaaat gatagaaaca
    23881 aaaagatcag agaagaaaaa aaccagccta atttccaata acgaataact tgttgatcta
    23941 gtcgaaacaa ttctgctctc ttgatttgca gatgactgaa tttcaaactc cataatatga
    24001 tcagatagac gaaaaacatc gaaaaggatt gctccgtact aaccaccact aatggcatgg
    24061 ccaatccgca tgccaatatc aaaaagatca acggaaaaat gcaagaatag cgaaaagctc
    24121 gttccaaata ggtttgcatt tctttttctt taggaagtaa aaaaacaata tctgctgcct
    24181 ctgcaaaagt agccatctta cccacgaata aacagagcat ccaaaataca gagattatga
    24241 gccctccaat tgcaaacggt ggctgcaggg ttttcaacca attagaataa tataatccca
    24301 caccgccaat caagaaaaga caaactaaaa caaaatgatc gttcaataca taacgcatat
    24361 atttcatcat tttcttttga tgtcttgcta aacgtttttg aaaaaaacta ctcatgtgcc
    24421 tgctcctttg ttaaagcaat atagatttcg tccaaagaag aatccggcag ctgaaattct
    24481 tttctcaaca tctcaagaga accgtctgct cgtatctctc cgttatgtaa gaccacaaaa
    24541 cgatcacaat atttttctgc agtagctaaa atatgcgtag acatcaaaat accggctcct
    24601 tcttttttca tttctaccat caattcaagc aatgcattga tagctagtgg atccaagcct
    24661 aaaaatggtt cgtcgatgat atataaagaa ggctcgatca aaaaggcgca taagatcatc
    24721 actttctgtt tcattccctt tgaaaaatta gctgggaacc agtcaagacg attagataac
    24781 cgaaaagtct ccaacaactt ttctgctctt ttcattgcta tatccatagg tatatcgtag
    24841 gccatcgcag tgacttcgat atgttctctc aatgtcaatt cttcatataa agaaggagtc
    24901 tctggaatat acccaatttg tttgcgatat tcttccgtat ttttctttaa ggatagatga
    24961 ttgatttcta tcgttccttt tgaagcttcc agcagaccga taatgtgctt gatggtcgta
    25021 cttttccctg ctccgttcag tccaatcaac cctactatct ctccaggctg tacatcgaaa
    25081 ctgatatctt ttaacacagg gagatgtccg tacccccctg tgacatgatc gataattaaa
    25141 ctcattcttt ttcctccgcc atttattttt atttattata ccacgaagaa aatgacggta
    25201 ttttgttttt ttccttacaa tagctttaac ttatggtaaa gttaagctga agtatttatt
    25261 ctttatagaa aggatgtttt atatggaaaa ctgtattttt tgcaaaatca tcaatcaaga
    25321 aatcccaagc tataaaatat atgaagacga caaggtctat gcttttttag acatctctca
    25381 agcaacgaaa ggacacacat tagttgtgcc taaacgtcat gtagcagata tttttgaata
    25441 tgatcccgaa cttgcaggag aggttttttc gcgtgtgcct aagattgctc aagcattgga
    25501 aaaagctttt ccagaaatga aaggtcttaa tatcttgaat aacaaccgtg aactagccta
    25561 tcaatctgtt tttcattcac atatccattt agtccctcgc tacagcaaag aggatgattt
    25621 ttctattcac tttgtcaatc atcaagatag ttatggatcg gaagaactaa aagctattca
    25681 agaaacaatc gtaaagcagg tgtcctgcga tgattaaaaa ctttttcaaa ggactgttat
    25741 ttggagctgc tgctggcact gtcggaggtc tgttattggc gcccaggagc gggaatgaaa
    25801 caagaaagaa actgatcgaa gaattggaag aagcaacaac tctgacaaat gatttaaata
    25861 acagcctgaa tcattttaaa agcgcattga tcgaaacaaa agaaacagct gaatctgtga
    25921 ttcctgcttt ccaagaagca atcaaaaaag atatcgaaga ttttaaattc caagccgaac
    25981 cacgtatcac acaaatcaat gaacaagtag aaaaactaaa agctgatctg ccagagtatc
    26041 ctgaacaaat ataataatga ttattttctc aacagattta actatataag tgagaaaagt
    26101 ttgcgtattt cctaggaatg tgatgaaatt ttgaattttg tttgaaacta ccacgacaac
    26161 gtttttaaag gaaatgggat atggtatagt taatatataa aaataataac taattacagg
    26221 agtgcaaatt ttaatgaaaa aaaaatcaat tattttggca gctactagtg cgttggctgt
    26281 tttaactcta gcagcttgtt caggcgatac taataaagat atcgcaacga tgaaaggcgg
    26341 aacgatcact gtatctgatt tctatgatga agcaaaactt gaatcatcga accaatcctt
    26401 agtacaacgg atgatcattt ataaagtatt caataacaaa tatggcgata aagtaaccga
    26461 taagcaagtg gatgcagaat acgacaaaca agcgaaatca ttaggtgata cttttgaatc
    26521 acaattagaa gctgctggat atacgaaaga tacttataaa gaatatattc gtaacaattt
    26581 agcttttgaa gctggcttga aagctcacgt agatattaca gatgacgatt tgaaaactgc
    26641 ttggaaatct ttccatccag aagtagaagc acaaatcatc aaattatcaa gtgaagatga
    26701 agcaaaagat gtgaaaaaat cagcggatga tggagatgat ttctctaaat tagccaaaga
    26761 caaatcaact gatacagaga caaaagaaga tggcggtaaa gttaaattcg actcaacgac
    26821 aacaactatt cctgcagaag taaaagaagc agctttcaaa ttgaaagacg gtgagatctc
    26881 cgatgttatt actacaacaa atcctacttc ttatgcgaca gaatactacg ttgtcaaaat
    26941 ggtgaaaaac caaaataaag gcaatgacat ggataaatac aaagatcaac taaaagatat
    27001 cgctactgaa actaaactaa gtgacaatgc attcacaact aaagtaatcg gtgaagaatt
    27061 aaaagatgca aatgtaaaaa tcaaagatga tgcctttgaa aatgtgttga gtgcattcac
    27121 aacaacttca tcaagtacga aagactcaag tgaaactaca gcttcaacaa agagttctga
    27181 cacaaaatca actgattcaa caaaagaatc ttcaactgaa gaaacaactg attcatcaaa
    27241 ataaaagaaa acaaaaaaag cgaagactta tgtctcgctt tttttgtttt gaatgaaaaa
    27301 aataaatttt tgctataaat cttttggtac atagaaactt cggttgtcca taccgaaaat
    27361 ccgatcggtg tattttcctg gttctgtttg acgaatcgaa gtcaacatca tctgcatcga
    27421 agcatcgata ttgtcgattt gatgtaatat ttctgcttcc attattcttg gacgtacagg
    27481 tgagccatat tctaataagc catggtgtgc taaaaccata tgacgtaata caagaacatc
    27541 ttcgtcagct tcgctgattt tcaacgcaag acaggcttta gttatttctt cgtcaatcaa
    27601 taccagatga ccgatcaaat ttcctgcaag cgtatattct gtcgacatag gaccggaaag
    27661 ttcaatcacc tttcctaaat catgcaaaat gataccagca tacaacaatg ctcgattcag
    27721 ctcaggatat tcatcggcga ttgatttccc taagtgaagc attgtcaaag tatgataagc
    27781 taatccactg gcaaatgcat gatggtttct ttttgctgcc ggaaactcaa aaaattgttt
    27841 ttgatattta ttaagtagat agcgaacaat acgattccaa tgcgcattgg tgatctcaaa
    27901 caacaattga ttgatttctt cttccatttc ttctttcttc aaaggggctc tttccatgta
    27961 taaactaggc tctcctggct catctaccct tgttaagcgc atatgaagaa tctttacttg
    28021 agggtttcct tgataaacct ctcttttacc gtttagaaaa acgactgtgc cggctgtaaa
    28081 tttattgatt tcctcctccg aagcatccca gtatttacca tcgatagtac ctgaagtatc
    28141 ttggaaagta aaagcaataa atttttttcc gtttttagcg attcttacat ctgcactttt
    28201 gatcaggaca aattgttcaa actgctcatc gattactaaa tcacgaattt ttttcattat
    28261 ttgctccttc taattgtttt acgatttggt tcatctcatg gtaatagctt accatttctt
    28321 tatcagaaga aaaacaaatt atttgatact tttctccaaa tttagctaaa agttcagcta
    28381 attgatactt tcgctgatga tcataatgaa gccagccatc atcaatgatg atcgggcata
    28441 tcgaacggtt agcttctaat gagaggtaag caaatctcat cgccataatc agctgatctt
    28501 tcgtacctgt agataactca tagatgggaa attctgtata tccgtctgtt aaagtcagtg
    28561 tatcattttt cagtaatatc ttctgataat ttccttttgt taaaatacct aaataatcac
    28621 ctgctgctct taacaactgg ggtaattgtc tttcactcat ctctgttgaa agatcaccta
    28681 agaatgtccc ggcgatttca agtgctcccc attcctccag taattcctgt aattttgctt
    28741 tttgccggct agcttgttga taaagttcgt ccaatgtgcc gtctctttgc aactcttcta
    28801 cttgaagttg gagtcgttgc cgattttcac ttgcttcttt ttcttcttgc tggagttggg
    28861 attgcttttc tttcagttgg cgcagtctta cagataattt ctcttttgtc agctcttcag
    28921 gaaatagtgg agtaagcatt tccgataatt cattgtatct tttcatctgc tgttcggctt
    28981 gtttggcttt ttgtaaaaag acaggtactt ccgaaggata agataagcct gcttgagtca
    29041 attgttcgct gtattcttgt atccatactt tttgtttctt tttcaattga ttgatttctt
    29101 gcttcaataa agtattttgc tgatagcttc tggcaaattt catctgttcc atctcttgta
    29161 taaaacggga aagcaatacc attttttctg aaattgtctt atcttgtatc ggtaaccatt
    29221 ctttaacaaa ctcaaatttt tggtcgattt gtcgtagttt ttcttgtatt tcctgttctc
    29281 ttttatgttt ccgctcaaat acgccatata atttctgata atctatgact tcctgtccat
    29341 attctttcat gagtttgagc gtatccatct ttcctaagtg ccgatgatcc gcaaaacgat
    29401 atacttcttg ctgaagaatc tgtttttctt ctccagttct cttttgtttt tcttctgctt
    29461 cagctaattg agctaaggct agatcaagtt gtcctagttt ttcttgccac attcctttta
    29521 tctcagagaa atcattttta gttttaattt ttggttgcct tttgtagaag aaatagccaa
    29581 tcatgcctgc accaattagt aaaaggatca ctttcaaggg cattggtaca aatatacctg
    29641 caatgattat tgctccgcca gccatcagca tatacggttt agatgaagag actggtgcta
    29701 tctctgactg attttgtaat aatgctggat aggtctcttc caagtcattc acttctgctt
    29761 caatcatctc ttgctgttct ttagctattt ggagacgcac agaaatctgg ctttctagat
    29821 cagcaatcct tctttcttca gccagaagat gcttccattc ttcttcatat aaaaattctt
    29881 ccggtaacgg atttttctgc caatgccacc tttgttccaa aatcttcaat tctgaatttg
    29941 tttctctagt ctcaatttgt aatctttgcc attcttcgtt taattgttcc gctgtaaatt
    30001 gaagttgctg gagatctttt agttcctgct cctgctctaa ataaaaataa tatctggctg
    30061 actgctgatc catcccgcta tgcttttcca gtgttgcttc taattcttcc aaacgctcat
    30121 ttgtttgctg ataccgtttg gagaaagact ccagatctaa aagaagttgc tcatcaggaa
    30181 gacctgtagt gctgtttttt ttgagagatt gccattcttc atataaagaa aaattaagtt
    30241 cttgctgtct ggctaatgaa aattgtttgt tcgcctcttt taattgtgcc aataactgct
    30301 gctgttgcga ctcgtattcc tgttcttgtt ggatcaactt gccaaaataa gcttcctgtt
    30361 cttgtttttg atgaattttt tcttgcagct ctttccattc atttaatttt tgattgatta
    30421 gtaatttttg gccttttttc ttgaataatt gttgtgcttt ctgataatat tcttgccgct
    30481 tttggaatac ttgagaactg cccgtgatgc ctaaagaaat cagcgcatca tgtagctctt
    30541 tttctctcaa atgatctagt tccatcaact gctcttgctg gaaagtaaat acctgctgga
    30601 ataactcttt cgtcaaaggt gcgatcagtt tttctagtaa ctcatcactt cccacttgat
    30661 cccccagcca caccttcgat tttccgcgat tttgctgctt gtagcgctcg acagcaaatt
    30721 caccatatac aggatgttta agccatattt tgccgccata ggctgagccg tcttttggtg
    30781 tataatcctt ttttttacgg ccttttgaag ggaaaccaaa taatattgct tgaataaatt
    30841 gataaatcgt tgattttcct gcttcgtttc ccccatagat cagctgattg cctgaataaa
    30901 agtcaaaact tttttgacgc cattttccaa aagcagcaat ctcgatcctt aaaatcttca
    30961 ttcctgatcc tcctcaaata aaaaatcttg attaagcagc tgcttggctt tttcaagtgt
    31021 ttcttcttga tattccggca gttcgctcaa tattctagcc gcttcttgat gactatatac
    31081 ttcatttaaa atttgttgga aatcttgagg tgtctgatag tattgaaata actgttccat
    31141 caatttagca gaagcagcaa gtggggtctt cgtataaaaa gtattctcta tgagagaaat
    31201 acgccaaata aagaaatcat ttatcctatc tgagaattcc tctaacaaat agtctaatag
    31261 ctctcctgat tgtatccgtt ccaaaacatc tgtattgaga tggtcataat cttttaattt
    31321 tatctcaatt aaactaaatc catcctctac ttttgaaagc tgctgacgaa tgtgaacaat
    31381 gacctctttt gtcgttcttg ctttcctcaa tgatatttcc tttgtcttcc aatagacctc
    31441 tgctacttta atcggatgta tttggcaact tcctttactt aattcaacta gtagaataga
    31501 tgtagaagct tgttcctttt tagtatgacc ttgaggcgca cctggataaa caattgtttg
    31561 cttttcattt aagaccgttg gcacatggat atgccctaaa gcccaataat cgtatccctt
    31621 ttcttgcatc tttgagggtt ggaaaggagc atagtttcct ttttgagaga cgccaggttc
    31681 cccgtggtac aacccaatat ggtaatctgt gagttctcta gaaggaaatt ccatcacttt
    31741 gtctttttga atccacggtt gacgataact aaatccactg agagagacag tttctccact
    31801 ttttatcgtt attttttttg tctctaccgt ttcggatgta aataaatgga catttttagg
    31861 aaaggagaac caataccgct ctttttgata gaaatcatga tttccgaata ttataaatac
    31921 aggtatatgg ttcttttcta accgtttcat ctgttctgaa aaatgtttct gtatttttaa
    31981 tgaaggacga ttttgatgaa aattatctcc tgcaagtaaa acaaaatcaa cgttatttat
    32041 tattgcctgt tcaatgatat ttgataaaac ggtcagattc gtcttcaata gtttttcctg
    32101 tacagtttta tcaagagttg tcagtccttc aaatgaccga tccatatgta aatctgccgc
    32161 atggataaat ttcaccatta aaaatttcct cctttttttc ttttatcata ccattttcaa
    32221 tgtttttttt catcaataac cgaaaatttg ttcgtattat gcaaagaaat tacgataaat
    32281 ggccgacttc aaaaaataag tttattcgaa aaaaagattg tgacatgatt tttgtcacaa
    32341 tcctaaatat ctatgttata ttcgcttcgt ttgacactga agaagtacta tttgtactgg
    32401 aatttatgct ccatttttcc gtatttagaa ttctatgttc tatactacct taattagttt
    32461 atatggtaag aattctttca aatgcttgag atactccgtc tttatcattt gaaacagtga
    32521 cataatcagc ttttttcatg atttcttgat gactatttcc cattgcaaaa ctaatatctg
    32581 cactttcaaa catagataag tcatttaaac tatctccgat cgcgattctc aaaggtttct
    32641 ttaaattgta gattgattcc aaacgatcaa acaccacacc tttagataca ttttgaggta
    32701 cgatttcaat atttacaggt gccgattcag taacagacac atttagcatc tttttcaaat
    32761 ggttaaatat attagctttc aattgacgat taggaataaa aataaaaaat ttgtaaatgc
    32821 tgatagcatc cttttctata atttcctcaa tattattata agggattgaa caaatagatt
    32881 tttcatactc aagctcatag tttagctctt tgatagaaaa ttcactccct acctcgtgaa
    32941 aaattttttt cattttattt ttataattct gagattcata acttccgaga tttgtatata
    33001 ttttggttgg aaatttcgat aggacagaat caattttttt tacatcattt ttatcgaagg
    33061 aagactcaaa tatctttttt tctccatcaa atccaacaga accatttaac cctactgctg
    33121 gtatccttaa accagatttt ttgagaatat tattaatgtc ttctacttct cttccagaac
    33181 aaatgaatgg aaaacagtct tccggtaaat gtttcattgt tcttgcattt ttttgactaa
    33241 tgctctggtc agaattcaaa agagtaccgt ctaaatctat accaattagt ttcatttcat
    33301 ccatgtctcc tatacgttga ttataaattc actgcttatt gtaagtctaa atttctcaca
    33361 aaacatatac taattatacc taatttaagt aagtacaaac atcattttaa ga
//
LOCUS       DAEGIQ010000021        33082 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000021 DAEGIQ010000000
VERSION     DAEGIQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.021"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(109..489)
                     /gene="yajC"
                     /locus_tag="KMT45_000896"
     CDS             complement(109..489)
                     /gene="yajC"
                     /locus_tag="KMT45_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBE7004377.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(586..1731)
                     /gene="tgt"
                     /locus_tag="KMT45_000897"
     CDS             complement(586..1731)
                     /gene="tgt"
                     /locus_tag="KMT45_000897"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBE7004378.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1948..2646
                     /locus_tag="KMT45_000898"
     CDS             1948..2646
                     /locus_tag="KMT45_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBE7004379.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2814..5453)
                     /locus_tag="KMT45_000899"
     CDS             complement(2814..5453)
                     /locus_tag="KMT45_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBE7004380.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6072..7103)
                     /gene="queA"
                     /locus_tag="KMT45_000900"
     CDS             complement(6072..7103)
                     /gene="queA"
                     /locus_tag="KMT45_000900"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBE7004381.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7296..8471
                     /locus_tag="KMT45_000901"
     CDS             7296..8471
                     /locus_tag="KMT45_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBE7004382.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8475..9116
                     /locus_tag="KMT45_000902"
     CDS             8475..9116
                     /locus_tag="KMT45_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004383.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9113..10030
                     /locus_tag="KMT45_000903"
     CDS             9113..10030
                     /locus_tag="KMT45_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE7004384.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10027..10698
                     /locus_tag="KMT45_000904"
     CDS             10027..10698
                     /locus_tag="KMT45_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004385.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11239..11727)
                     /locus_tag="KMT45_000905"
     CDS             complement(11239..11727)
                     /locus_tag="KMT45_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBE7004386.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11928..12194)
                     /locus_tag="KMT45_000906"
     CDS             complement(11928..12194)
                     /locus_tag="KMT45_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBE7004387.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12194..12457)
                     /locus_tag="KMT45_000907"
     CDS             complement(12194..12457)
                     /locus_tag="KMT45_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBE7004388.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12933..13118
                     /locus_tag="KMT45_000908"
     CDS             12933..13118
                     /locus_tag="KMT45_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE7004389.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13260..15083)
                     /locus_tag="KMT45_000909"
     CDS             complement(13260..15083)
                     /locus_tag="KMT45_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBE7004390.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15680..18385)
                     /locus_tag="KMT45_000910"
     CDS             complement(15680..18385)
                     /locus_tag="KMT45_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBE7004391.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18691..19638)
                     /locus_tag="KMT45_000911"
     CDS             complement(18691..19638)
                     /locus_tag="KMT45_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBE7004392.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19828..20097
                     /gene="rpsN"
                     /locus_tag="KMT45_000912"
     CDS             19828..20097
                     /gene="rpsN"
                     /locus_tag="KMT45_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBE7004393.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20273..20635)
                     /locus_tag="KMT45_000913"
     CDS             complement(20273..20635)
                     /locus_tag="KMT45_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBE7004394.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20646..21767)
                     /locus_tag="KMT45_000914"
     CDS             complement(20646..21767)
                     /locus_tag="KMT45_000914"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBE7004395.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21783..22133)
                     /locus_tag="KMT45_000915"
     CDS             complement(21783..22133)
                     /locus_tag="KMT45_000915"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBE7004396.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22263..23774)
                     /locus_tag="KMT45_000916"
     CDS             complement(22263..23774)
                     /locus_tag="KMT45_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBE7004397.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24096..25448)
                     /locus_tag="KMT45_000917"
     CDS             complement(24096..25448)
                     /locus_tag="KMT45_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBE7004398.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25601..26677)
                     /locus_tag="KMT45_000918"
     CDS             complement(25601..26677)
                     /locus_tag="KMT45_000918"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBE7004399.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26873..28618
                     /locus_tag="KMT45_000919"
     CDS             26873..28618
                     /locus_tag="KMT45_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBE7004400.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28898..29335)
                     /locus_tag="KMT45_000920"
     CDS             complement(28898..29335)
                     /locus_tag="KMT45_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBE7004401.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29319..30692)
                     /locus_tag="KMT45_000921"
     CDS             complement(29319..30692)
                     /locus_tag="KMT45_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004402.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLRLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31014..31502)
                     /locus_tag="KMT45_000922"
     CDS             complement(31014..31502)
                     /locus_tag="KMT45_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7004403.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31524..32126)
                     /locus_tag="KMT45_000923"
     CDS             complement(31524..32126)
                     /locus_tag="KMT45_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBE7004404.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(32195..>33082)
                     /locus_tag="KMT45_000924"
     CDS             complement(32195..>33082)
                     /locus_tag="KMT45_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBE7004405.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT         9448 a         7030 c         6060 g        10544 t
ORIGIN      
        1 attcaatatt gagcaatata tcccatgctc ttatctcttt cttttttgaa taaaaaatga
       61 ggtaggacat ctttctgccc tacctcgtct ttaaataagc tttgatactt atttttcttc
      121 cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa ctgtctcagc
      181 tggttcttct gttgtttcaa cagtagtcgt tgcatcattt gttacgacag tccctggttt
      241 gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt ctaaaacaac
      301 tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga tcgttacgac
      361 tgaatcacct ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt gtttcttttg
      421 cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa gcattggtaa
      481 tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt agcagaaaaa
      541 tgctgataac actagttttt tacttaattt ttaaaagatt ttcctttaga agctttttgc
      601 gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa gcaaattatc
      661 atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata aattgtggta
      721 agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga tataggcacg
      781 tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga aatcatgggc
      841 atatttcgca tttttcacta ctagacggcc ttgagaagtc atacatgtgc catttcgagc
      901 gattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac catcgatcaa
      961 cgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga tcaacggtgt
     1021 agtgaattct aatacacgat tcatttctga tttaggttcc ccgacagata acccgccgat
     1081 tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac gaagatcttc
     1141 aaaccctgct ccttggatga ttccaaataa tccttgacgg tctggattag catgagcttt
     1201 caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt agtcgtaact
     1261 ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca atttattttg
     1321 gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt gatttttgaa
     1381 atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt gaaagccgcc
     1441 tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc cagcttcttc
     1501 tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa taacgccagc
     1561 acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg ttcctactgg
     1621 catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta aacgcgcgcc
     1681 agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca ttcatttccc
     1741 tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca agtgcttttt
     1801 gcaagttccc tcggcttcct tctactttat ttaatattct ttttttatcc tttttttatg
     1861 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
     1921 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
     1981 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
     2041 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
     2101 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
     2161 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
     2221 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
     2281 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
     2341 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
     2401 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
     2461 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
     2521 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
     2581 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
     2641 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
     2701 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
     2761 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
     2821 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
     2881 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
     2941 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
     3001 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
     3061 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
     3121 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
     3181 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
     3241 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
     3301 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
     3361 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
     3421 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
     3481 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
     3541 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
     3601 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
     3661 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
     3721 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
     3781 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
     3841 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
     3901 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
     3961 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
     4021 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
     4081 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
     4141 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
     4201 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
     4261 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
     4321 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
     4381 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
     4441 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
     4501 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
     4561 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
     4621 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
     4681 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
     4741 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
     4801 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
     4861 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
     4921 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
     4981 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
     5041 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
     5101 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
     5161 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
     5221 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
     5281 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
     5341 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
     5401 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
     5461 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
     5521 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
     5581 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
     5641 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
     5701 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
     5761 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
     5821 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
     5881 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
     5941 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
     6001 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
     6061 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
     6121 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
     6181 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
     6241 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
     6301 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
     6361 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
     6421 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
     6481 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
     6541 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
     6601 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
     6661 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
     6721 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
     6781 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
     6841 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
     6901 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
     6961 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
     7021 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
     7081 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
     7141 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
     7201 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
     7261 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
     7321 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
     7381 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
     7441 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
     7501 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
     7561 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
     7621 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
     7681 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
     7741 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
     7801 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
     7861 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
     7921 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
     7981 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
     8041 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
     8101 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
     8161 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
     8221 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
     8281 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
     8341 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
     8401 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
     8461 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
     8521 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
     8581 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
     8641 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
     8701 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
     8761 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
     8821 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
     8881 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
     8941 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
     9001 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
     9061 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
     9121 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
     9181 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
     9241 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
     9301 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
     9361 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
     9421 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
     9481 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
     9541 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
     9601 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
     9661 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
     9721 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
     9781 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
     9841 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
     9901 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
     9961 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    10021 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    10081 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    10141 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    10201 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    10261 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    10321 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    10381 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    10441 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    10501 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    10561 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    10621 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    10681 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    10741 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    10801 cttatgggca aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    10861 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    10921 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    10981 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    11041 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    11101 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    11161 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    11221 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    11281 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    11341 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    11401 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    11461 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    11521 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    11581 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    11641 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    11701 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    11761 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    11821 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    11881 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    11941 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    12001 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    12061 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    12121 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    12181 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    12241 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    12301 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    12361 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    12421 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    12481 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    12541 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    12601 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    12661 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    12721 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    12781 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    12841 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    12901 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    12961 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    13021 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    13081 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    13141 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    13201 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    13261 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    13321 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    13381 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    13441 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    13501 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    13561 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    13621 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    13681 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    13741 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    13801 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    13861 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    13921 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    13981 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    14041 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    14101 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    14161 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    14221 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    14281 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    14341 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    14401 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    14461 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    14521 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    14581 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    14641 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    14701 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    14761 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    14821 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    14881 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    14941 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    15001 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    15061 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    15121 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    15181 aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg
    15241 ctttaataca gcaaaaatct aacatttttt atgggctttt tctttgaatt ctataataaa
    15301 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
    15361 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
    15421 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa
    15481 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
    15541 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    15601 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
    15661 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
    15721 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
    15781 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
    15841 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
    15901 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
    15961 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
    16021 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
    16081 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
    16141 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
    16201 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
    16261 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
    16321 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
    16381 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
    16441 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
    16501 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
    16561 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
    16621 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
    16681 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
    16741 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
    16801 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
    16861 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
    16921 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
    16981 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
    17041 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
    17101 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
    17161 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
    17221 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
    17281 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
    17341 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
    17401 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
    17461 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
    17521 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
    17581 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
    17641 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
    17701 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
    17761 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
    17821 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
    17881 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
    17941 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
    18001 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
    18061 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
    18121 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
    18181 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
    18241 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
    18301 tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
    18361 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
    18421 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
    18481 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taaggattac
    18541 agcctttact ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta
    18601 ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc
    18661 agcttttttc gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc
    18721 agcaggagga tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga
    18781 cagattcatt gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc
    18841 aaaggcttct gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt
    18901 tttttgccgc aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt
    18961 catgatctgg atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc
    19021 tgcctgatca gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc
    19081 tcctaatttt tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc
    19141 cttttcatgg agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat
    19201 tacagtatct gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc
    19261 tggcgtgacc attccggctg cttcttccca actcatttta tctggaattt ttgctacttt
    19321 tgctgatggc aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg
    19381 atcacctaca tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc
    19441 attacctggt acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa
    19501 ggcaacatca taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc
    19561 gacttctctg ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt
    19621 aattgcaaat cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt
    19681 tattttgaca aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc
    19741 ttttttcgcg taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta
    19801 ataaaaaata attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa
    19861 aaacagcaag aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa
    19921 gattacgaag gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt
    19981 gatttaatcg atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa
    20041 ttccgtgaat tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc
    20101 aagtctggct gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa
    20161 gatacgttcg cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac
    20221 gcacagctgg tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag
    20281 taacaggagt cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca
    20341 taatgggtcg atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga
    20401 tctgctccaa caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa
    20461 tgtgagtcgg aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg
    20521 ggctaaaatg gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct
    20581 ccgatcccac gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat
    20641 cctccttatt cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag
    20701 tggatcgtac ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg
    20761 ccgataagcg tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga
    20821 tccatacata cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc
    20881 tgcatctttc gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct
    20941 tcaggagtga tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact
    21001 tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg
    21061 tgcccagagg gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt
    21121 tcatcatgcc ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga
    21181 agttttttca gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc
    21241 tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg
    21301 atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca
    21361 gtgtctgctt ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca
    21421 gctgctgctt gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct
    21481 acagtcagtg cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct
    21541 tctctcaact cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca
    21601 actgctgcct cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca
    21661 aacaattctt ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct
    21721 tttttatgta tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct
    21781 ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc
    21841 catacattgc cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa
    21901 atctcgacat cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc
    21961 tctttgcaag cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg
    22021 aatagtttca tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc
    22081 agccgagtga tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt
    22141 tctcttttca ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa
    22201 ataagtccac aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat
    22261 gtttaatcgt tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg
    22321 ccatcttttt ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa
    22381 ttaccgccgt cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat
    22441 ccttttttct gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct
    22501 ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct
    22561 aataatttat ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg
    22621 atttgttcaa ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt
    22681 aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt
    22741 ggtgtaacga aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg
    22801 tgaacgtagc tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa
    22861 atatctaatc cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta
    22921 aatgaacgca atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca
    22981 cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg
    23041 aagacgattg ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt
    23101 tcgtattctt ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct
    23161 ttgatcttca catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc
    23221 ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt
    23281 tcgatatctt ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt
    23341 tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta
    23401 cccacaacga tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg
    23461 ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt
    23521 gtttggatag ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct
    23581 ggatcgattt tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt
    23641 gcttgtccaa taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc
    23701 gttgcttctt caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt
    23761 tctttaaatt tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc
    23821 taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt
    23881 ttttatccat tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat
    23941 tagaaaagcc aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa
    24001 gttgtttcta tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta
    24061 acatagtttt tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct
    24121 accacttcaa ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca
    24181 agattttgtt tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt
    24241 agttgggggt atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg
    24301 aaaatcatgg cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa
    24361 gactctttcc ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc
    24421 agatttttaa aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg
    24481 ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa
    24541 acatgggcga gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta
    24601 tacgcaatca aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct
    24661 ccttctacta caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg
    24721 ccatttccaa taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc
    24781 aacggttcat ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc
    24841 attttggctt tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc
    24901 attgtgattg ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg
    24961 tgatccatgc ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg
    25021 taaggaagac caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca
    25081 gacaagacag acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca
    25141 acgacttctg cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa
    25201 ggatctgcta caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa
    25261 aatgaagcag atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta
    25321 ccagcaagag gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg
    25381 gtaatctttg tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt
    25441 agattcattg agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg
    25501 gttcgcatga ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa
    25561 cgcggttacg caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa
    25621 aagattgacg ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac
    25681 cgtatttcaa gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg
    25741 gcatagagtt taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc
    25801 ggctcaatcc gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt
    25861 gataaacttc ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt
    25921 ttgcttcata atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa
    25981 cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga
    26041 ttgctcgaga atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt
    26101 ctgcttttgt aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa
    26161 gcaaagaacc ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa
    26221 gtactggtac ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga
    26281 ttttatccat ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg
    26341 tctctaagaa gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa
    26401 cgatggctcc ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct
    26461 ctgtctgtcc acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg
    26521 ttagtaatgg acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt
    26581 aataattata ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct
    26641 ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa
    26701 atcgttcagt gatttactct catattttag cacaaaacaa aaataaacga ataacttatt
    26761 tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat
    26821 tatttgtccc gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca
    26881 aacaaatcca cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt
    26941 gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg
    27001 ccccaccttc atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc
    27061 cttcactcaa acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt
    27121 caaaaagacg atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc
    27181 aaaatcgcct tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg
    27241 tgacgcggta gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct
    27301 tcaactattt gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga
    27361 tctaaaaggg gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa
    27421 agaaagtcaa aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag
    27481 ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg
    27541 taaaaacttg aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat
    27601 tcaaaacaag cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca
    27661 ggtgtttgat gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt
    27721 actaacaaaa gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga
    27781 gttcggttcc tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac
    27841 catcaactac atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact
    27901 cacacaagcc gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct
    27961 tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag
    28021 ttttcttgaa cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac
    28081 acatgtcgtg acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt
    28141 gctgaaagca tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct
    28201 catggaagtc gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga
    28261 tacattgaaa caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc
    28321 tcaacagctc caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga
    28381 attgaaaaag caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt
    28441 cggtatgatt ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt
    28501 agaaaacgca gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt
    28561 cattattttt ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa
    28621 aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt
    28681 tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag
    28741 aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca
    28801 tggaacacat ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat
    28861 accttcgtta cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg
    28921 cttgacgaaa agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc
    28981 cagcttttct taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt
    29041 cgtcaaattc ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag
    29101 ctaaaccata atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat
    29161 aatccgcact atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt
    29221 tggtcgtata atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta
    29281 attttatgac atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt
    29341 ttcgccttct tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg
    29401 atccattttg ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc
    29461 gtgatcaata atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc
    29521 atcaataaaa actggtggat cacactccct ttattttgag tagtatactt gacaacgatc
    29581 cggcgatcca ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct
    29641 gttactacgt gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag
    29701 aggaccaata aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa
    29761 cgtttcaaaa tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa
    29821 tacataaaaa tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat
    29881 aaaaaaagga aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc
    29941 aacttccatt ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagccgcaag
    30001 aaaccataaa tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa
    30061 agaaacaata agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt
    30121 cttttgatgt ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc
    30181 atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac
    30241 tcaatcgctt gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc
    30301 atattgataa aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc
    30361 catgctgcac tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg
    30421 cctaatattc caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg
    30481 taactatcta gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca
    30541 taaaacagcg taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat
    30601 aaaagataag gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa
    30661 ttacgcccaa atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa
    30721 ctaatctgct cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt
    30781 cttgtaaaaa aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga
    30841 ttactcaagc agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca
    30901 ggtgataaag atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa
    30961 ccagagtcct tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg
    31021 agagcttttt tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt
    31081 tcttctgtaa tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga
    31141 ttcactcgca aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga
    31201 aaaactgaag aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa
    31261 taaatttttc catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt
    31321 aataaaaagt aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa
    31381 gttttcagca tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac ttccggccat
    31441 acttcttgta tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc
    31501 atacacccac gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc
    31561 atgatcatgc tatacacacc caccgaccac ggtttcaaga aaatcacaat ctgcataaat
    31621 tccctaaaag ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag
    31681 ggaagcagca aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat
    31741 tcatcaaatt tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag
    31801 taacggacaa gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa
    31861 agcccaaatc cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa
    31921 ataggaatga tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca
    31981 aattgctgga taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca
    32041 taaagaatca acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt
    32101 tgtaaaactg atcctttttt agacatctat attccccaca tcttttttca tttattaaaa
    32161 ctgcctcctt atgaactgaa ccccaaaagt tgaactattt aatggactgt ttccgatatt
    32221 ctactggaga tagtccattt aactttagtt ttatcctttt gttattatac cacctaatat
    32281 actcatttaa tttactttga aatatctcaa ttgatcggaa tttttctcga taaaaaaact
    32341 ctgatttaag cacaccaaaa aaattttcta ttacagaatt atctaagcaa tttccttttc
    32401 tagacatgct ttgaataatg ttattctctt ttaatttttt ttgatattgt ggcatctgat
    32461 actgccatcc ttgatctgaa tgtagaatca gtgaaccctc agttcccttt tttttaattg
    32521 cttgttgaag catttcttca atcagtttat atgttggact agttgatata ctataactaa
    32581 ttatttctcc gttaaataaa tcaagtatag gagatagata gatttttctt ccttttatct
    32641 tgaattcagt gacgtctgtc acccattttt tattgggtgt atctactgaa aaatttcgtt
    32701 ttaacacatt cttggcaatt tttcctactg ttcctttata agatttatat cgctttattc
    32761 ggatttgaca ggtaagtccc atttgggaca ttaattttct aactgtttta tgattgattg
    32821 tatatccttt catctttaac gctaaagtga ctctacgata accataagag tttcttgatt
    32881 cttttacaat cgctgtaatt tcttgcttta tcttgctata tttatctggc ttatctaatt
    32941 tttttaccca gtaataataa gttgacttcg ctaattgtgc aattgaaagt aatagattca
    33001 atttaaattc ttttttgagc tgaaggattg ttttaacctt gatttcttct tgctcaaatc
    33061 ttgttcttga atcaaggttt ct
//
LOCUS       DAEGIQ010000022        32987 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000022 DAEGIQ010000000
VERSION     DAEGIQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.022"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            15..1568
                     /locus_tag="KMT45_000925"
                     /pseudo
     CDS             15..1568
                     /locus_tag="KMT45_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1676..2758)
                     /gene="uxuA"
                     /locus_tag="KMT45_000926"
     CDS             complement(1676..2758)
                     /gene="uxuA"
                     /locus_tag="KMT45_000926"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBE7004406.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(2749..4395)
                     /locus_tag="KMT45_000927"
     CDS             complement(2749..4395)
                     /locus_tag="KMT45_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBE7004407.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4579..5241
                     /locus_tag="KMT45_000928"
     CDS             4579..5241
                     /locus_tag="KMT45_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7004408.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5385..5948
                     /gene="ahpC"
                     /locus_tag="KMT45_000929"
     CDS             5385..5948
                     /gene="ahpC"
                     /locus_tag="KMT45_000929"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBE7004409.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            5958..7487
                     /gene="ahpF"
                     /locus_tag="KMT45_000930"
     CDS             5958..7487
                     /gene="ahpF"
                     /locus_tag="KMT45_000930"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBE7004410.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            7644..7994
                     /locus_tag="KMT45_000931"
     CDS             7644..7994
                     /locus_tag="KMT45_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBE7004411.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            8093..8623
                     /locus_tag="KMT45_000932"
     CDS             8093..8623
                     /locus_tag="KMT45_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBE7004412.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            8777..9928
                     /locus_tag="KMT45_000933"
     CDS             8777..9928
                     /locus_tag="KMT45_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBE7004413.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(10037..12076)
                     /locus_tag="KMT45_000934"
     CDS             complement(10037..12076)
                     /locus_tag="KMT45_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBE7004414.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(12134..12691)
                     /locus_tag="KMT45_000935"
                     /pseudo
     CDS             complement(12134..12691)
                     /locus_tag="KMT45_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(13230..13436)
                     /locus_tag="KMT45_000936"
     CDS             complement(13230..13436)
                     /locus_tag="KMT45_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004415.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(13452..14066)
                     /locus_tag="KMT45_000937"
     CDS             complement(13452..14066)
                     /locus_tag="KMT45_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBE7004416.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(14213..14896)
                     /locus_tag="KMT45_000938"
     CDS             complement(14213..14896)
                     /locus_tag="KMT45_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBE7004417.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(15005..15463)
                     /locus_tag="KMT45_000939"
     CDS             complement(15005..15463)
                     /locus_tag="KMT45_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBE7004418.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(15547..17310)
                     /locus_tag="KMT45_000940"
     CDS             complement(15547..17310)
                     /locus_tag="KMT45_000940"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBE7004419.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(17531..18991)
                     /locus_tag="KMT45_000941"
     CDS             complement(17531..18991)
                     /locus_tag="KMT45_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004420.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(19199..20125)
                     /gene="glsA"
                     /locus_tag="KMT45_000942"
     CDS             complement(19199..20125)
                     /gene="glsA"
                     /locus_tag="KMT45_000942"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBE7004421.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(20148..21359)
                     /locus_tag="KMT45_000943"
                     /pseudo
     CDS             complement(20148..21359)
                     /locus_tag="KMT45_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(21394..23007)
                     /locus_tag="KMT45_000944"
     CDS             complement(21394..23007)
                     /locus_tag="KMT45_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7004422.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(23119..23328)
                     /locus_tag="KMT45_000945"
     CDS             complement(23119..23328)
                     /locus_tag="KMT45_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBE7004423.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            complement(24317..25531)
                     /locus_tag="KMT45_000946"
     CDS             complement(24317..25531)
                     /locus_tag="KMT45_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBE7004424.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(25659..26129)
                     /locus_tag="KMT45_000947"
     CDS             complement(25659..26129)
                     /locus_tag="KMT45_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBE7004425.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            complement(26126..26368)
                     /locus_tag="KMT45_000948"
     CDS             complement(26126..26368)
                     /locus_tag="KMT45_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBE7004426.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(26914..27444)
                     /locus_tag="KMT45_000949"
     CDS             complement(26914..27444)
                     /locus_tag="KMT45_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBE7004427.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(27626..28282)
                     /locus_tag="KMT45_000950"
     CDS             complement(27626..28282)
                     /locus_tag="KMT45_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBE7004428.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(28409..28882)
                     /gene="dfrF"
                     /locus_tag="KMT45_000951"
     CDS             complement(28409..28882)
                     /gene="dfrF"
                     /locus_tag="KMT45_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBE7004429.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(29301..29519)
                     /locus_tag="KMT45_000952"
                     /pseudo
     CDS             complement(29301..29519)
                     /locus_tag="KMT45_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29550..30764)
                     /locus_tag="KMT45_000953"
     CDS             complement(29550..30764)
                     /locus_tag="KMT45_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBE7004430.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(31105..31347)
                     /locus_tag="KMT45_000954"
     CDS             complement(31105..31347)
                     /locus_tag="KMT45_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBE7004431.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(31379..31936)
                     /locus_tag="KMT45_000955"
     CDS             complement(31379..31936)
                     /locus_tag="KMT45_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBE7004432.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(31949..32137)
                     /locus_tag="KMT45_000956"
     CDS             complement(31949..32137)
                     /locus_tag="KMT45_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBE7004433.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(32150..32716)
                     /gene="amaP"
                     /locus_tag="KMT45_000957"
     CDS             complement(32150..32716)
                     /gene="amaP"
                     /locus_tag="KMT45_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBE7004434.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         9641 a         6588 c         5478 g        11280 t
ORIGIN      
        1 aaggagcctc tttcatgcaa actcaagaag cgattaaaac gtttatcttg aagaaaaaaa
       61 gcaatacaaa attagatatt tttcttttcc tatctgaaca tcgtttcttt attactacac
      121 aatatttagc agatcatttc catatgtctg aatcaaattt tttactatac atcaaagaat
      181 tagaacaaga ctttgagcgt ctcaatctaa cagaactgca tattgataag caaaaacctt
      241 ttttaaagtt gaattttgaa ggaattgatc cggcttactg ctattatcgt ctatttggaa
      301 gatactgcaa cgaatcagtc agttatcaga tcttgacttc tttgttcagc tgtcagacaa
      361 attcgattat cagtttcagt cagcaaacaa actatagtgc ctcttatttg tatacgaaaa
      421 tgaaaaagat taatgcattt ttagcatagt acggtttgtc tatctccttt tctaataatg
      481 gcagaaagtc tttttctgga aaagaggtac aaattcaata tgcagcatta gatatttatt
      541 ggaacatctt ttcaagtact gctacagaat tttatgatga agatccgcaa gtagtggctt
      601 ttatgatgaa tacttttttt aaaaaagaag tacttgaccg actaaatagc ggtatgatag
      661 ataaattata ttttctatta aaaatatgta aagagaattt tcctgcaact tctgtgaaag
      721 acgtacaaaa agaattcaca gagtatgaat atattgactt ttttattgat ctctctgtag
      781 atattttgcg acctaatctg ccaatttgtc gcgaacaacg catcttagtg aatattttag
      841 ctcgactttc tatcacgaaa atcgaatcgg aagaaacaag cttggagcaa tacaaacttt
      901 taaaaaaagc aaacgttcct catgttcttt attcaagaga aatggtggag tccctttgta
      961 ctgtatttga tttgtttata cctgaaaaag aaaagattct ttactcattg gcattcgcac
     1021 gaaataaact ttataatgga tttcttaact tcaatcaacc aaatacgccc cttcctactt
     1081 tcatgcttta tcaagaacat agcgtattga aagaagtcca aaccgccatt gaacaatttt
     1141 atatggattt cagagaacaa aatcaacact tgttaccagt tattttagaa aaggaaaatg
     1201 tgcaatggat ggttgaagac ctagtacatt tgtatgatcg ctacaaaaaa cagccgcgaa
     1261 ttgtgattgg tgtgaactat acgcgtgatt tttatatcag taaagatctg atcgttaaga
     1321 tagaacaaat tttttcaaat gaatcgatca aaatacaaaa tgattttatg gagaactgca
     1381 acatcgttat cagcgactgc cctttgaaac aattgccttc tcatatcaaa aaaatctata
     1441 ttttaaaagg aatcatcaca ccagaagact ggaaaaaagt gattatgagg atctcagaat
     1501 atatctttga attaaaagaa gaagcactca tggatcaatc gaaaaaagaa aatcaagtta
     1561 cttcctagac caaataagat acataaatcc gaatgcttat taacaacctc cggatttatg
     1621 tatcaagaat ttgaacatcc ttcttattta cataataaaa ttacagtttt tttcactaat
     1681 tatctttact tttgtttcct ttttcgattg cttcccataa tccatttaga taaacagcac
     1741 ctaatgctcg atcatataag ccgtatcctg gtcttcctgt ctcattccag atcattcttc
     1801 catgatcagg acggatgtat ccgttaaatc cgttgtcata cattgttttt agtatgcggt
     1861 acatgtctaa tgaaccgtat tcggatttat gtggtgcctc atagaaatct cgattagcta
     1921 aaaatttaat atttcgaaca tgtgcaaatg ggattcgatc tctttggcaa aattctgcta
     1981 aaatcccata tacatcattt tctggatctt ctgcaatact ccctgtacaa agagtgattc
     2041 cgttataagg gctgtctact atgcgacaca accaatctaa atcttctcga ttctttacga
     2101 ttcgagctaa tccaaaaatc gagtatggcg gatcatctgg atgtacagcc attttgaccc
     2161 ctacctcttc acatgttggg atgattgatt ggagaaaata ggcaaaattt tctcgaagtt
     2221 tttcttcgtc tactgatgca taggcttcaa gcaaggattt gataggagct aagcgctcag
     2281 gttcccatcc cggcaatcca aattcattgc tggaatgttc gatgcgcgcg acgatttctc
     2341 ttggatcatc tggaatatca tcagatataa aagctaacgt ggatgagccg tcttctaagc
     2401 ggtagtcaag gtcagatttc acccagtcaa atacaggcat aaaattatag cagatgactt
     2461 ctattccata ttctgccaag tttcggattg tctgcttata gttttcgata tactgctcac
     2521 gggaaggtaa tccaatttta atggcatcat gcacattgac gctttcaatt acttttagtg
     2581 ttaacccacg agactcaatt tcttctttta gttctttgat cctctcccta ggccatacct
     2641 ctcctactgg tatatccatc aatgtgccta caatcccttt gaccgcagga atctgtctga
     2701 tttcatctaa ggtaatcttt tcttcctgtg atccgtacca tctaaaagtc atttccatga
     2761 taaatttcct ccatgttctt gtaagtaatc ctgtaatgtt tttcgtacgg catttggtcc
     2821 tgctaccatc ttgatgaaca tttcttctat ttttctgcca agctctgctt ggtaaagatc
     2881 tacaccaaaa atttctttat ttgacaaaat aggagccatt ttctcatgaa tatgtgatgt
     2941 atcttgcgta cccagttgaa tatcggaaag cttatgctga aggtctgtca atagtgggtc
     3001 tggactcggg atgaatggtt ttccttgatc atcaatgcca ataaggtatc gaagccaagc
     3061 agcgagagtc aatggtatga atacaagtgt ctgcgggtct ttaacttcta tttccatata
     3121 ttttttaatc gtctcaccat aacgaatcgc caccttttga gaggtatccg tggcgattcg
     3181 ctgaggcata ttaggaatat ttggattaga aaagcgcaca gtaattactt cttccagaaa
     3241 gatctttgga tcaatgatac ctggagactc cacaactggg aggccttcat tgtacccgat
     3301 ttcagaaact agcagcgata aatctggatc tttcatctcc tcagctatgc tcctataccc
     3361 cagtaaacaa cccataatag ctaaagacgt atgcaaagga ttaaggcaag tcgtcacctt
     3421 catcgtatct gctttatcta ccgttgcacg atcagtcaag aataccccag ctttttcgaa
     3481 tttcggacgt ccattgggaa aatgatcttc catgattaaa taatggatgt cctctgtatt
     3541 gacaaatgga gcaatattat ttccttttgc ggtttggaaa atcgacatat ttgaaaagcc
     3601 ctcttttgcc aacattttgc taactgacaa ggaaggattc ggggtgatac gatcaatcat
     3661 cgtccatgga aacgcaacaa tttcttcatt cgataagtat tggataaatg tattttgtac
     3721 ataaccttcc ttttgccatt cttttgcgat cgttagcaaa gaagtacgta attgactacc
     3781 gttttgtgaa aaattatctg tagagacaag tgcaatcggc caagctccag ataaaaaacg
     3841 tttataaagt agcacagcaa gaatactcat tgtatgtgat ggcttctttg gaccattttt
     3901 tatatcgact gcaatcggtc ctatatattt attatttgta tctttcaatc gataaccttt
     3961 ttcagtaata gcaaaagtaa ctaattgtaa actaggattt tcaaagtgtc gaatcatctt
     4021 atcaaagtgt tcagaacaat ctggatgaca atacagactg tcagctgtcg cagctaatat
     4081 acgtttgacg aactctccac tgcttggcat gaccacttca agaatcaaat catcaaaatc
     4141 ttggtaagcc ctttcaatta tttcttcatc gaatgtttca caaacaatga caccagtgga
     4201 caattcgcct agatccgcta aacgttgagc gatttctgca tgaaatcctc gataaagatt
     4261 tccgccaccg aaatggatcc agacaggatt tttttgtcca ttttctctta atgatgcacg
     4321 gtcaaaatga ggtatttcta cttctttttg agataattca gggataaagt tcctccagtt
     4381 gtttaaatca atcatttttc ccctcctaaa ctaacatatt agtttttgta ttcaagtgaa
     4441 ttttatgcaa taaaaatcgc tatgtcaacc aaaattttac tatttataca aatttactag
     4501 ttaatgtgac ataccagatt gtttttcaga cctcttctgt tatactacgt tcatagaaat
     4561 gattttggag gaataaagat gaaaagcagc ttgcaatcac aagcttatca agcgattcgt
     4621 aaacaaatta tctatgctga cttagaacct ggaaggaaaa tatctgaaaa ggcactggag
     4681 gaacatcttg ctatcggccg gactcccatt cgcgaagcat tgattcaact acgtcagcaa
     4741 gaactcgtgt atacgatacc tcaaagtggt acgtatgttt ccaaaattaa tcttgtttct
     4801 gcagaaaatg ctcgctttgt ccgagaacag ttggaagagc aaatcatgat tgaatgttgt
     4861 tccaaattgg acacacaaac acgaatagtg ctagaaacga ttttagaaga acaagaaaaa
     4921 gcagtcaaaa taaatgacga acgcgctttt ttccgtgcag ataatttatt tcatgaagct
     4981 tgtttcgaaa ttgcaggaag aaaagaagtt tggaactggt tagatgacca taatacccat
     5041 ttagaacgtt tccgctggtt acgagtgacc attcaggggc ttccatggga aacgatcatg
     5101 gaacaacatt atgatttact caaggcttta gttgataaaa aaccagaaga agctcgattt
     5161 caaacgggat tgcacttaca catgatgctg aaagaacagg aaactgttac tcaacgttat
     5221 cccgagtatt tccagcagta gcattttctt gcgacctctt ctatttttat aattaacctt
     5281 cgtttttgag taatctttat taaataacaa aatatccatt gactttgcaa ctccaactaa
     5341 attacaataa ttataaataa ataattatta ttggaggttt tactatgtca ttgattggga
     5401 aaaaaatcga agaatttaat gcacatgctt atcatcaagg agaattttta gaagtcagtg
     5461 aaaaagatct tatggggaac tggagtattc tttgcttcta tccagctgat tttacttttg
     5521 tttgtcctac tgaattagaa gacttacaag aacaatacag tacattgaaa tctttaggtg
     5581 ttgaagtctt ttcttgctct accgataccc actttactca caaagcatgg catgatacat
     5641 cagatgctat cggtaaaatc gaatacgtga tgatcggtga tccgtctcat cagatttctc
     5701 gtatttttga tgttttggat gaagagcaag gattagctca gcgaggaaca tttattattg
     5761 atccagacgg aatcgttcaa gctatggaaa tcaatgcaga tggaatcggt cgtgacgcta
     5821 gtgcactgat tgataagatt cgcgctgctc aatatgtacg tacccaccca ggtgaagttt
     5881 gtccggcaaa atggaaagaa agtggcgaaa cattgaaacc aagttttgat cttgttggaa
     5941 agatttaggt ggtggatatg ttagacaaag aaactaaaca aaatctcgaa caatatttag
     6001 cactgattga atctcctatt gttttttctg ttagtttgga taatagtgaa aatagtcaaa
     6061 aactagctga atttacgaag gaaatagcag aaatgtctcc taaaatctct tgggaaaaaa
     6121 gagattcgat gcgcacacct agtttttcta tcaatccagt tggcaaagaa agtggaatcg
     6181 tttttgctgg tatccccctc ggtcatgaat tttcctcttt tttactggct atgttacaaa
     6241 ttagtggacg ggcaccgaaa atcagtgaat ctttgagtct taaaatcaaa aaaataacac
     6301 aaacattgca ttttgacagt tatgttagct tgacttgtca taattgcccc gacgttgtac
     6361 aggcattgaa tatattagct gtattaaatc caaatattac tcatacgatg attgaaggtg
     6421 ggatgttcca aaaagaaatc gatgataaaa aaatcatggc agtgccaact gtgtttttaa
     6481 atggagaaga atttagtagc ggacgcatga cgatcgaaca aattgttgaa aaaatcactg
     6541 gccctttgat tgaagaagag ttgtttgaaa aagaacctta tgatgtatta gtgattggcg
     6601 gtggaccagc agctagtagt gcagctatct atgcagcacg taaaggaatc cgtacaggac
     6661 ttgtcgctga ttctttcggc ggacaagtcg ttgaaacact agggattgaa aatatgattg
     6721 gtacctctta cacagaaggt ccaaaattga tgcagcaagt agaaagtcat gtgcgtagtt
     6781 accctatcga cattatgatg aatcaacagg ccgtttcctt aaataaagag caacatttta
     6841 ttaatattgg cttagctaac ggtatatcac ttaaagcaaa aaccgccatc atcgcgacag
     6901 gtgcgcactg gcgttctatc aatgtaccag gcgaaaaaga gtttaaaaat aaaggaattg
     6961 cttattgccc acactgtgac ggccctttat ttaaaggaaa agagattgct gttatcggtg
     7021 gtggtaattc tggtgttgaa gcagcaatcg atttggctgg gttagcaaaa catgtatatg
     7081 ttctagaatt cttaccagaa ttaaaagcag atcaaatctt acaggataaa ctagtctctc
     7141 ttgcgaatgt cactgttatc aaacatgcag cgacgaaaga aatcagcggt acgaatcagg
     7201 tagaatctct ctcctatatc gatcgccgta cgatggagat gcatacacta gctgtttcag
     7261 gagtattcat tctagtcggc ttactcccta atacggattg gctagataga acgatcgaaa
     7321 tgaatccaag aaatgaaata gtgaccgaga aaaatggcgc aacgaatatc cccggcgttt
     7381 tcgcagctgg tgattgtaca gatagtccat acaaacaaat cattgtttca atgggttcag
     7441 gagctactgc agctttagga gcattcgatt atctgatgcg cacttgagct atgaagattg
     7501 taaaaagtca gagagcggaa aaaaggatgg tgacaagaat tttgtcacaa tcctttttgt
     7561 ttttttgaat aatcgctttt tcttctcttt ttcttatata ctataagtag taaataattt
     7621 gaaaaaaaga aaggtgactg attatggcgc aaaatcgtat ttttacgaca agttttgcaa
     7681 gcgtttatcc tatgtatgta gcaaaagcgg agagaaaaga tcgtataaaa gaggaagtag
     7741 atgaaatcat ttgttggctg acgggttatt cccaaacaga actagaatat caactacaaa
     7801 aacaagtgac tttcgaacaa ttttttgcgg aagcccctgt ccccaatcct tcacgttcct
     7861 tgattacagg tgttgtttgt ggtgtacgag tagaagacat cgaggataac ttgatgaaag
     7921 aaattcgtta tttagataaa atgatcgatg agctagcaaa aggcaaaaaa atggaaaaga
     7981 tcctcagaag ttgatttttc cagagatatt cttctccttc gctcattttg taacatttaa
     8041 tttggtaaaa tgactagttc acgttaaaat aagtcaaagg aggttttctt atatgtttga
     8101 gacgattaaa caaaatattc aaaaatacgc cttattaaga gctgctgttt atattatttc
     8161 tgggattgcg attgtgctaa acccttctgc tgtttttcat tttatcggtt atctgatcac
     8221 cgcctatctt gttttgcttg ggttgttgaa tctttttgag gcttaccgtt ttaaaaggaa
     8281 aacaggttct tggagtatcg gtctgttcag tggtcttttt ttcctgatct tagctctgat
     8341 cgtctatgct tttgcacctg caatcgtttc tatcctgccg attcttcttg gattagctgt
     8401 tatcatcaac agcttgttcc agttgttctt cagcatgaat acaaaagcta aaagttggag
     8461 tgtttatagt agtattctgt tgttgattgg cggattcgtc ttattgttca atccattcaa
     8521 aagtttgatg cttctattcc aaatattcgg ctttattttg attttcatgg gcatccagga
     8581 gattatcaat ttcatcagaa taagaaaaat gactatacgt taatcatttt tagttattaa
     8641 aatagaccgt ataaaaacca acaatccaaa aaaatctttt ggattgttgg tttttttgtg
     8701 agtatatttt cctatttttt caataatgta tgttatgcta agttcgggct atattttata
     8761 caaaggaatg atgagaatgg aatttattgg tattttatgc ctgatattga tttcaacaac
     8821 gattggctca catatctcgc ggagattagg tatcccagca gtgattggac aattacttgt
     8881 tggtgttttg ctaggccaag ctggattgaa ttgggtcaaa cctgatattc tagtccatga
     8941 cttttcggag attggtgtga tcttattaat gtttttagct ggtctggaaa gtgatttatc
     9001 tcttttgaaa aaatatttcc gacctggtat gttcgttgct ttgcttggga tcatcttccc
     9061 ggttgttttt ggctggttga caggcaaagc attccaagtt tcgggaaatg aagcgatttt
     9121 ctttgggatt attcttgcgg caacttctgt cagtatttca gtagaagtat taaaagaact
     9181 aaacgtagtg aatacaaaag aaggatcaac aattttgggt gcttctgtgg tagatgacat
     9241 tttagtcgtt ttagttctaa gtttcagcct atcttttcta acgggggaag cttctagcaa
     9301 tgcttcttta ccgcttgttt tgattgaaca gctaatttat tttgttttta ttttcttatt
     9361 agtcaaatgg attgcgcctt tcctactttc atttgcagaa aaagtgtacg caaattcttc
     9421 tattatcatc gtatcgctag ttatttgttt aggaatgtct tacgtagcgg atcttgttgg
     9481 gcttagctct gtcattggtg ctttttttgc tgggatagct gtcagtcaga caaaggttaa
     9541 agaagaagta taccacaatg tagaggcttt aggatatgca gtatttatac cagtattttt
     9601 tgtcagtgta ggattagaag ttgatttttc tgactttagt gaacaactac tctttatttc
     9661 tatattgacg cttgtagcga ttttgaccaa attgcttgga ggttattttg gtgctcagat
     9721 tgccggtttt tctcaacata gttcattgat ggtcggtgct ggtatgattt cgcgtggaga
     9781 aatggctttg atcattttac aaattggaca gcaaagtcaa ttgatcgaac cacagtacta
     9841 ttcaccttta gttatcgtga ttttattaag tacacttatt tctccacttg ttctcaaata
     9901 ttttacaaaa aaagtttatg aagtgtgagt aaatcaaaaa gaagttgtga aagagacatc
     9961 gtgaaaaccg tttatttgaa aaaaggactg tgacgataac gccacagtcc ttttactgta
    10021 cttaaaaagc aattttttat tgataatttt ggttgaggta ttgcaatagt tccgatgctc
    10081 gtttggttgc tgaatcatca tcttctttat tttccaacat gctgaccatc atataacctt
    10141 ggttatcaag attgaaagca aacaagaaac tgttctcttg cccctttaca tcctgttttt
    10201 ctttgatttc cgctgtacca gttttcgctg ccaacggaat ccctaaagca gaaagagaat
    10261 gtgctgtacc atttacatct tgcacaactt ctctcagatc tggcacgatc gtttgtactg
    10321 ctgtttcgct gatgacattc ttcttatctt ttgtctcttt atctgcaatc aatttaggat
    10381 agacaagtgt gccattgttg gtaaaaacag aatacattgc tgcttgctgg ataggagtaa
    10441 ttagaagttc gccctgtcca tatccagtat cagctagcaa gatatctgag ttaaagctat
    10501 cttcattaga aatttgtgct ggattcatac taattggcaa atcaaggtct tcaccaaaaa
    10561 tgaatttatc taagcctgta cgaaattttt tctcacccat tttcaacgtt tcttgtgccg
    10621 catatatatt atcggaatag atcaaagcag tttttaagtc tacttgtgat acatcactac
    10681 tgacacgcgt tgttttaaaa gatccccaag aactgtcttt ttgccattta agcccgttga
    10741 tcgtcaacac ttcatttgga tcgatagtgc cgttgtcgag accgattgct gctgtaatca
    10801 ttttaaacgt agatccagga gcataacctg tcgcaaatcg gctgatgaat ggatgttcag
    10861 gattttcttc ataagctttg taatcttctt gtgagatccc gtttgtcatt ttgtttggat
    10921 catagcttgg agagctagca agcgcaagaa gatcaccggt ttttggcgtt gtcgcaacag
    10981 ttgatgcagc ttttcctcct agactgtcaa aagctatttt ttgtgccttc gcatcgattg
    11041 tcaatttgat atctttgcca ttttgaactt catgttcgat cagaactttt ttctctacac
    11101 cgtctgcatc tgtaatgctt aatttcccac ctgttgtccc acgaagatcc ttatcaaaag
    11161 ccatctccaa acctgagcga ccgatttttc cattactgct taattctgga ttcttatcaa
    11221 tatcttctgc tgtgatatct cccacgtaac caatcagctg agcagctgct tcacccaaag
    11281 gataatatct gccgtctact tcttgaatgg tagctccagc tggaagttct ggcgttgctc
    11341 catcaatgat tttcaatggg acaaagtaat cgggttgtac ccagctttgt gaaatcgcct
    11401 gattgatagc atcttctgtt aagtcgaagg aggaagcaat cgctttgata ttggctgttt
    11461 tttcgcctcc atctccaagt ttacttggaa cgactcctaa ttgttttagt ttgcctgttg
    11521 ttgctaatgg ttcaccattt cgatctataa tattccctct tgctgcttct tgtgtggtca
    11581 ggcttacttt gtcatttcct tccatttctg gaaaaaccaa gttgggttgc cagttgatcg
    11641 tggtttgacc atcatttctg tctaatgttc ctttataaga aagatctttc aattcaccta
    11701 atgaggtatt catctttgct ttatatgaaa aactgtaagt agaatcgtct tttttatcta
    11761 cttttagatt cgatatctga agtcctttga catcagcagc accgtatata ttctggtatt
    11821 tatctaagat ctctttttca gataatgcac ttttatttgc cgcctttttt gatgtcattt
    11881 ctgcagcttt gttataatct cccttgttca aagcttggac aaattgctca actgtctttt
    11941 ctccagcttc tactgcttgg gtttcttgat agtgctggta gtaaaaataa ccgccacccg
    12001 cagctgctat taagatcact gcgccggcaa tataagcgcc tgttcgattt ttgccgtgct
    12061 tgtcacttct tttcattttt tcctccttct cttgcttgtc attcctttat ttcactattt
    12121 atacctgttt attctaacat atgtctttga ggaaatccat accacaatcc agaagcacac
    12181 tttctggaag gttcgtttgg atactaccaa gtaatttccc aaccgctttg ggtagcttga
    12241 atattgaagt tacgtctttc agctgctgag agatagcttt gatcacttgc tgctgccttc
    12301 tgatcctccc gaagtctcct tctttgtctt cccgaaatcg agcatactgg agtaaagtat
    12361 taccatccat cacttgctct cctttgttta tatatacgcc atctaaatct agagattttt
    12421 ctgcatcgat cttgactcca ccaggaaaca gttcattaac gcaatcgata aaatcttgaa
    12481 atgtgatact tgcataatag ggagcttcaa atttgaaatt ttcctttaat gtttgattta
    12541 ataaatcaac tcccccataa gagtaagctg catttatctt attcattccg taacctggaa
    12601 tatcgacata tgaatctctc atgatagaga tcagttttgc ttgttgtgtt ttctggttgt
    12661 attgtgcaac cagaagtgaa tccgagcgac ctccaccaat cgatcttttt tcaccgcatc
    12721 tcctcctcac acagcaatcc acttgtcagg tgaggagcga gtttgtctca tcaaataagt
    12781 cagtcacaaa aatataaaga aaaggtcaga aaccttttcg aacaagaatt ctgacctagc
    12841 atcatctcat cgtccgagtt ttagcactat acaacgtaaa gaaaacattc ttagctattg
    12901 gaaaaagcct taatccaaca gtttctgttt tacccattgg cgactttcga cgtttctggg
    12961 caacagccta tgtttgtata ggagcctcac ctaacaaggt tattactata ttctatttac
    13021 gagttcatga tagacttttc ctaatgatta ggcaatggaa tttgaaatga aatagtgaaa
    13081 tttaacaatc tttaatattt tttagaaaaa aataacaaat aagttgaaag aatcccgttg
    13141 caaattcttt cagttgcacc aggattcttt gataattttt tagttttatg tgttttttta
    13201 tatgtttttt cacttttcgg aataaatatt tatttgttaa agtaattaaa caatccgcta
    13261 atcgtttgat ctggcatttc tatttcctta ccatctaaag taacagtagt aacgacaaaa
    13321 tggtcttgca atggcggaac ttctaagtac tgcccttgca gaaaatcatt cactgtttca
    13381 tattcttttt tcatttcttt tgaatctgtc gatggtttat agcttatttt taacataact
    13441 agtgctcctt tttattcggg acgtgcaatc gtgaaaattt ctcctattgc tgcataatca
    13501 tttcctgcca aacggcttac agcattcaat tttcttggat cgatccttcc tgtcttatca
    13561 tcatagactg tttcatttaa atgatatttc tgtacttcta gcaaaaataa atcagctgtt
    13621 actttatttt tattccgtat ctcgacatgt tcatataagg atgtttcaaa acgaactttt
    13681 gcttctgtca atgctggtac tgaaatgatt actgaatcag ttaaagtcaa gtcagtcagg
    13741 tacagttcgc tttcctcatt aggcagattg gctgctgttt tatttacttc ttctacgttg
    13801 tcctcatcaa ggatatggat aacagcttct cttgtctcaa gaatattctt tgctgtgtct
    13861 tttactgcat cttgttttct ctggatcgcc aaggataaga tgggtcgatt cgaactgacg
    13921 atattgaagt aactgaaggg tgcgatattc actatatctt ttgctgattt agtcgttact
    13981 actgctactg gtctagggat gactgaacca atcagcaact tgtaattttc acgttctgtc
    14041 aattctgaag gactccgttc aaacatggtt tcgccttctt tctataaaat tcgttaaaaa
    14101 gcagatcttg aagacaaaat gaaaagtaat gcttttttca tcttcttaga atctgcttta
    14161 aactatctat caatttttct tttttgtcaa gaatctgtgc cgtgcgtgtt ttttagttat
    14221 ttgctttact tgcggcttcg acatatcttt tagtgatcaa ttgcggacgg gtgataatag
    14281 atccaactac gactgaaaaa gcacctaatt catatgcttt ctctacttgt tcaggagagt
    14341 cagtatgtcc ttccataatt actgggatat cgacttgttc tatgatttga ttcaataaat
    14401 caaaatcagg tgtctgcgtc tttcgtgaat attctgtata gcctgctaaa gtcgtagaca
    14461 aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc aaaagtcgaa atatctgcca
    14521 tagctaaccg accgtgttcg tgaatcattt ggacaagttc agcagttgtt ttttctgtcg
    14581 gacgtttacg agaagttgca tccagtgcaa tgatttctgc ttgcgtctcc aaaagttctt
    14641 ccacttcttt ttctgtcgca gtaataaaca cttctgaccc atcgtaatct cttttgataa
    14701 tccctattac cggtaaatca acgattcgct tgatttcatt gatatcttct gtcgtatttg
    14761 ctcgaatacc aactgctccg ccttcttttg cagccaaagc cattctcccc ataataaaag
    14821 aactgtgtag cggctcgttt tccaaagctt gacaagaaac gacgactccg cctttgattt
    14881 gttccagtgt atccacaata tctcctccag taatgttata taaaaataca gcttcatata
    14941 aaaatgtaac ccttttctat tcaggagaca ataatgagct atttctttca gcagaccttt
    15001 cttactaaaa gaaaattact tcaaattctt cgtatacgac cagcttctct tctgtaaatt
    15061 ctatgttcaa cgcttcttta taagcatgtt taaaaaactc agcatggact ttccgccaat
    15121 aagctaatgt ccgatcccct tctccttctt tccaagcatg ctctttcgtg acttcactaa
    15181 aaggcaagat ggtcacattt atatttttga tgatacaaac tggttcattt tttccattca
    15241 ggaccacgtc taattgacca acttttggta aaggctcatt ctcttgttta tacagatcgt
    15301 aaccacttga tgtaccagtc tttatctttt ttacaactag ttcgctcaac tcgtctgcca
    15361 ttttttctgt atccccaaat gaaaagaaac ctaatttttc tgattgataa ctaggatatt
    15421 tttcttggaa ctttttgaca tattcttcta cttgctgata cataacatcc acccctttgc
    15481 tttttataat gaaaaaacca tccagatact agtataaaca attatctgga tggtgagcat
    15541 aatgaattat tgaatattca agacacgcag actatcttca acgatttgtt ttggtgttaa
    15601 atgataacgt tcataaagct gccctacagg aacgctatcc gtgaattctt tctctgcacc
    15661 aaaatttaat acgttcattg aagaatcacc ataataacga ctgatatttt cgccaaatcc
    15721 tcctgacaaa ctaccatctt ctaatgtgat gactaattcg tgtgtttctt ttaatttatc
    15781 taatgtttct acgtcatttt ctgtgatcgt tcctggattg atcaacgttg cttcgattcc
    15841 tttttttcta aaagctttta ctgtttcttt tcctaattca agaaaaccgc ctaaagctaa
    15901 aattgctact tttcctccat tatttactgt ttgataggct ggaatactat aatcatttgc
    15961 gatagctgta ccatgttcta caccatgtgt aggaattcgg attgctacag gctgcgctgt
    16021 ttgttcgaac gcccaatcta gcatagagat catatcctct tttgttgctg gagcaagata
    16081 tttgatgttc gggatacttg ataagtaagg aatgtcaaat gttccttggt gtgttcggtc
    16141 actagctgag atcgttccgc caccgactaa aataatcact ggattctcgt tgatcgctaa
    16201 atcatgtgat aattgatcat atgcccgttg caagaaagta cttgattgga agacgactgg
    16261 acgtactccc tgagaagcca aagcagaagc gaaagtgata gagtgctgtt cagcgatccc
    16321 cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt ttcagtccaa aggcgcctgg
    16381 tatcgctgca ttgactgcgg caataggagc tgtacttgtt tgcattttct tatccaaata
    16441 atccatgatg atcatattaa atgtttctcc agaagttact tgctttgcct ccccggtgga
    16501 cagatcaaat ggtacatgcc aatgatattt cattttatta tcgattgccg gctgataacc
    16561 gcgacctttt tccgtatgga cgtgaagtac aatcggatga tcaatatctt ttacttcttc
    16621 aaataaacgg atcaccgttt ccagatcgtt tccattatcc acgtaacgat aatcaaatcc
    16681 cattgcttta aataggttat tgctgctttt accgttggtt tgacgaagct cagctaagtt
    16741 tttatataaa ctgccatgat tctcatcaat agacatctca ttatcgttta ctaatacgat
    16801 tagattggat tgtaaaacac caccattgtt caatccttca aaagctaagc caccacttag
    16861 ggagccgtca ccgataaatg ctaccacatt tcctgattct ttcttcagat ctctcgcttt
    16921 agctaaacct gttgctaagc tgatagatgt agatgtgtgc cccactgtaa aatagtcatg
    16981 tttactttca tgttggttgg aatatccgct cacttcacgg taatgtcctt tttcaaaacc
    17041 attttttcga ccagttaaaa gcttatgacc ataagactga tgggaaacat cccaaacgat
    17101 tttatccttt ggtgaatcaa atacataatg aaacgcaatc gttaattcta ctacacctag
    17161 attcggacca acgtgcccac ctacatcgct gtccttttgc aagatttttt ggcgcatctc
    17221 ttctgccaac tgtttcattt cctcaacaga taattttttg acatctgcgg ggctattgat
    17281 tgcatccaat acatgtaata ctgtactcat aatacattct ctcctccgta ctatcttctt
    17341 ttaatgaaaa tagatttgtt acactttatc tattctacta cttagagttg actctaagtc
    17401 aatagcattt taaaaaaatt tcagagaaag cttgttatat aagtagatct tcgcacgaat
    17461 caggactcaa ataaaaacct tctacagaca attaaaaagc tttgtagaag gtctcctaat
    17521 taaggtctca tcaagaaata tgttttttta ataggtgaag ttcctgagca atatgataca
    17581 aatggatgtt tgcacctttt aatgagattg gatagatctt tgtcttttca gtttctaatc
    17641 cattttgata gctagcagca tcgcacaaaa ccactctttt tttcgcacat agacattcat
    17701 caggcaattc ttctaatgaa cgaaataaaa taggatttat ttcagcgatg ccttcttccg
    17761 catacaaatg aaattgggtt cgataaaaaa attgtttgac tgaactttct gaaacaacaa
    17821 aaatttctat ttcctgtctc cccgcttgtt cactgttgat caaagaaaga cttttgacga
    17881 attttcgaat gaaattttca ttccatctta gttggatttg atttttcttt ttccactctt
    17941 ccaatatttc tacaattttt ccgtacagaa gttgttcttt ttctgataga agatagatcc
    18001 tttccggtaa aaatacttgt ccatctgccc acaacgtacg gacaaattgt aaaaaggatt
    18061 gcttgaaaat aaaattttct gcatcgtcga tttcagcgat actaataatc tgtaaaatca
    18121 attcattcag tgataggtca ttgagaacaa aatttttata aacaacttcg aaatcttttc
    18181 ttaacagctc catgttctca ttggtataat ttctactgtt gaacaaagaa aaaatataat
    18241 agacatcttc ttcttgtaag tctagaattc ctttttgctc ttccaatcct tgacagacta
    18301 attgaaataa cggcttcgtt tctgcgcttt tcttatcttc ttctttcagt ccaaggggaa
    18361 atgcctttct tccgattgcg atctctattt ctcgtatgat aaaggacttt tcatcttccg
    18421 aatatcttct tttaaaaaaa tgagtctcta cataatcaat caactcgtta caagcttctt
    18481 ctacatacgg atttttattt tcatagcctt cccaattgac ataacgtact aaagctagca
    18541 agatgttccg cgagtcaaat tctgggataa tgatttcttt atttttcaaa taatcatttt
    18601 ctttaaaaaa atccaatacg atttttttga ttgtatatac tttactcaaa gaaataaatt
    18661 caagatcagc cagttctgta ctcgtgaata taccattaaa ataataactt aaaaacttta
    18721 aaaaataaga ttgaccgtac agaaactgtg tgagctcaga aagagcaata ccatgtttga
    18781 atacgagttt atacttatgc ttctctctta cttgttcttt tatacaatat tctagacact
    18841 cggctagact ttctagatcg tgaaggatcg ttggtgtagt cacccttaga gaaatagctg
    18901 tttgttcgat gtctatttct tggaattcaa ataattgttc acacagatac acttttctaa
    18961 atatatttcg ctcgatgtaa ttttccagca ttcctgatac acgcctccag tttttcttat
    19021 gattttattt aatcatatct gtaaatcgta tttttgtttc ctttttattt agaaaagccc
    19081 tttcaaaaac caataaaaaa agaaactggg acaaaagtca aacaagctca caatttcagc
    19141 ctcattgtag gtagctttga gccgtttgga cttttacccc agcctctttc aagaatggtt
    19201 attcaaagat gtcagcgtct agttctttga ctacatcttt caacaattgg ataccagctg
    19261 cgctgttacc aaatggatct agtgccgggc tgaagacacc tatgccgtaa cgatttggca
    19321 cagctgccat caacccgccg cctacaccgc tttttgcagg tacaccaaca tggacggaaa
    19381 actctcctga ttcatcatac aaaccagcag ttgtcataat tgatttgacg attgtagcac
    19441 tttcttcagt aatgatttgt ttgccattcc aaggagcaat tcctttattt gccagcacag
    19501 cagctaattt tgctatgcct gtagcggtga cattgaggga gcattgtctg aaataagcat
    19561 cgagtatctc cggtacgtct ccttcgatca tttgattacc tttcatatag taagcaagag
    19621 agcgattgat atcacctgtt cttgattcag aatgatagat ttcttcattc aatgtgatat
    19681 taggatcatc acagatctct ttcataaatg acaagattcg ctcaaatttt tcatctgcat
    19741 ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc attaacaaaa ggattcattg
    19801 gatgtttgcg atgattgatt tccatattca tgatagaatt gaaggcaaaa ccggttggtt
    19861 ccgtgttgat tgtttgaaag actttttcta ttccgttgtc tttgattgct aataacaata
    19921 cgggaacttt tgacatgctt tcgatggcaa agcgcacatc ggcgtctcct ccctcaatca
    19981 gttgttttgt atccaaatca taaatcgcca tacctaattg tttgggatcc acttcagcta
    20041 atgctggaat gtaatccgcc acttgtcctg tttgataata gtggaaattt tttccaatca
    20101 attgttctaa ttcttttact tccatttgaa taaccagctt tcatttttta tttttctgca
    20161 tgctggaatt tatgacggca tgcgtagatc gtaaatggaa taactaagac gataagataa
    20221 gaaacaatca gaatcgtttc ataaatccca tgctgtccag ctgcgatact agcaggtgga
    20281 acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac ctaaacaagc aacgattact
    20341 tttacaactt ttccgcctgg aacctgatag gctctttcaa gactgtcatc tttaaaaacc
    20401 agcttcaaat agcccgcgaa aaacataaag tacaacatca ggtagattac aacagtcaat
    20461 gacatcgctg caagaaatga aagattattc cccccgccgc ttaatgtaaa gacggctgcc
    20521 cagattgttg caacgatccc atttgcgatc atcaaatgag caggaacacc gtgtgtgttt
    20581 tcttttgcaa aatattcagg aagtaatcct tcttctgctg ctacgtgaag tgcttttgaa
    20641 ggagaggtta cccaagaagc aatttctccg atgacaccta aacaaacaag aatcgcaaga
    20701 attttcgcaa tccatgcaag atttggatca atgtgtctta ataggaattc taacgattga
    20761 atcacccctg tattaagaga caaaccatgt tgaggtactg tagtagcgac ggtcaatccg
    20821 ccaaaagtat ctaaaatgat tgccagaata accaatacaa acattgctaa tggataatcc
    20881 tttttagggt tttccatttc atttgcatga ctggcagatg tttctacgcc catatatgct
    20941 aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg aaaaatcagg aatcaatgtt
    21001 ttcatagaca gtatcgtttg tagtggagcg cctgaagcga cataatggat ccctaagcca
    21061 aacaagacta atgctggcaa caagatacca gctacaaaac cgatccgagc aatcaatgaa
    21121 gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc agaaaataat caataggatt
    21181 gccaatttca gatatttatt gttttgtata gcaccaatac ccgttgcata ggataatgca
    21241 ccagttaaga aatacagcat ggtcaagtag ccaactgtta tctcaaacca ttggaaaaag
    21301 attgcggcaa aaccgaatcg ttttcccaat gtgcgactga cccaagtata gacaccgccg
    21361 gtttccctta gttaaaaata ttactcttgt tgattactca actcttccaa taaatcggta
    21421 atatatctca atttcctgta ttctttcgtt atcctcattc gttgttgctt catgtatgag
    21481 gattttttct atcatggcat ttaataatgg tgctgtcagt tcctttggta cggaatattc
    21541 cttgattaac tcaatccact tttcagcacc tatcatatcc tgttccattt tacttagttc
    21601 ttctcttagg cttgttatct gctgttccag ttctatctgt tctttttggt acttgctgga
    21661 caacatcaca aaatttcgct ctgttatctt ctcacaggct ctatcttcat acattttcgc
    21721 aaataaacgg tcaatctcat tttgtctgtt ctcggctttc ttcaatgtac ttgccttttt
    21781 cttcttttcc cgtattctct ctgcattgcc agctttctgt atcttgttca ataccttttc
    21841 ttcgtcctgc tgtactgcct tagcccaata ctgcaagcgt tccaatacgg cttgatacag
    21901 cacatcataa cgaatgtagt gcatagaaca attacctttg ccaaactgcc cgtagtaact
    21961 gcaagcataa taactgtaag gcgttttatt tgtcttattc gttccaaatc tcatagacca
    22021 accacaatcc gcacacttga caagccctgc aaaaatcggc gttgcctttt cctttgtctg
    22081 tctgcgtctt gattttatct gctcctgcac acgatagaac acttccttgt caataatcgg
    22141 ctcgtgagtg ttttctactc gaaaccattc actttcgggc ttacgcactt tctttttgct
    22201 cttgaacgat accgtagact gcatattgtg aacactgttt ccgatataaa cctcactttt
    22261 caatatcgcc ttgacatgag caatcgtcca ctcataacgc ttactttcgg gttttccctc
    22321 aaagatatgt gcaaaagtac cgtatcttgt aaaatttagc catgacgctg tcggaacttt
    22381 ttctgctctt aactgctttg tgatttttgc acttccgtaa ccttgatagg ctagggaaaa
    22441 gattttttca ataatccatt ttgtttcatc atcaaccaac agttttcctt tgatgtcagg
    22501 gtgtttctta tatcctaacg gagcataagc accgtaatga gccccctccg caaactttgt
    22561 cttaaatgct gatttgactt tacggcttgt atctcttgcc acccattcat tgatgatgtt
    22621 cttaaatggt gcaatctcac tttcgccttt ttcgctgtct acaccgtcat caatcgctat
    22681 gtagcggaca ttatgactag ggaaatacaa ttctgtatat tgtcctgtca taatataatt
    22741 tctgccaaga cgggaaaggt ctttcgttac aacacagttg atttttcctg cctctatatc
    22801 ctcaatcatt ctttgaaaac tcggtctgtc aaaatttgtt cccgaccagc cgtcatcaat
    22861 atattcatca atgacattca aatgatgttc ttttgcatat aaacgcaaca tacttctctg
    22921 tgtggtaatg ctggaacttt cgccctgtaa ttcatcatct cggcttaacc taagataaag
    22981 tgcagtattg taaatctgtt gtttcattgt cagttatcct cctttataac taacccgtgt
    23041 ttacaatacc tgcttgcaag taaagtataa cacgggtact ttttgtcatt caatctattt
    23101 cttacaactg caccgatttt atgcgattgt ctgctttgct ttttccagca tgacatcagt
    23161 tagaagttct tccattgttt tgcccttttc ggaaaaatgc tcttttacta caatctttgt
    23221 ctttcctctg ttgccaatgc cacacttgcc atttttcttt tgaattgttt ttacagcatt
    23281 atcaataaca cataccccct ttctgtaaaa tctttctaag caattcaacg gcttttgtca
    23341 tagcccacag gagttccacc tctgcatagt tcttatgtag ccgtacccat tgcgtgcgac
    23401 gctcttaatg ctcaagctgt ggctgtacgg gagtatcatt atctgacagt acaggtcatg
    23461 gcgataaaag aggacaaatc ttttacaaga acacaaatag gttttcgctc gctcttgcag
    23521 gggaaaggtc gtggcgtatt tgtttcaacg gctagctgtc tgtcaaacgt attgaatagc
    23581 tttattcagt tgtcaaagaa cgattgaaag aaaaaaatcg tctttcctat atatcgcgtt
    23641 ggaaaagagg agaaacgtaa ccaaaatcca aaactttttc cattattagt acaaatggaa
    23701 cggttcattt tggcaatcaa cggttgattt tctccttttt cactactatc tgtacagcaa
    23761 acgtccattt gctaagttta aatggaaaaa ttttaacttc ttttcatgca ccatataggg
    23821 atagaaaatg tcattttgct aagttgatga tgaaaaaaga atattttcct tttcacaccc
    23881 taactaccca aaccataccg ttactgccaa caatagaatg aaatattttc tttgcaccat
    23941 atagggaacg gaaaccttaa attgttaagt tgagaacaaa gtattttcat cttttcacat
    24001 caacactcaa ctgctaatgc ctgtttgaat acaaaatgat gatgtttttc ctcttttcac
    24061 agaagactgt ccactaatca cgaatttccg tcattttttc aaaacttttt tcttctttca
    24121 cttcatatag ggacagcaag ccccgattta atgacctatt ttcaaaaaaa gtttagattt
    24181 ccttttctcg caccatatct gtacgcaagt agccattttg ttgcagattt aataaaaaat
    24241 ttatttttct tctttcactt cacataggga atgaaaacag ctctttgctg actaaatctg
    24301 aaatttttca attacattaa aaaataccgt cattctcttg tggaacagcg gtatctatac
    24361 gttcttctat ggttgatttc tctaattgta ataaatgttt atgcttgtct tttagttctg
    24421 cctgctcaat catatctttc agtatcgttg tacgattttc tctgtctaat gcctgtacca
    24481 tttttatggt tggtgcgact tgtctttgta gccaatgcaa cgctttttgt aaagtgtaag
    24541 gctctggctt tgtggttaag cgtatcggct ctctgttttc ccctacaaac catgcccaat
    24601 catcatttgt tatccattga ctttttggct tgtcgtcctc tctgtcaaca aagcggacat
    24661 aatgattgat gatagaaaaa gcggtgcgtt cagcgtcccg ataggtcagt aaatctacaa
    24721 ccgcataata ggctcgttcg ttcttcatgc gtatttcaaa acggttctta atctccgtat
    24781 cttcaatttc tgtgccattc ttgacatact gctcataatt tttctgataa gcacacatat
    24841 ataattcgct acttgttgaa ccgagatata aggtttctcc tgtattcttt ggtaaatcgc
    24901 taccgcattt ttctgtaccg ctgtaatctt tctgcatacg gaagtaggac atacattcgc
    24961 cagccttgta tttttccttt aactttggaa tatccaatat tcctgcttta tcattgatag
    25021 ccaaatcaag ccgtttcatc acaccaccag ccgtcataca atccagcatg aagtcatacc
    25081 atgagcgttc ctgtgccagc agataacatt ccatttgccg acagcctttg cctttcagtt
    25141 ccaataaaac acctaaatgc tcctgcatgg aacacatgat attgatgtcg ccaagtacat
    25201 atttgctttc atatccgtat tttccataat cttcataaag catgtaatta gatttcagtt
    25261 gtaatacatc acggataatc gccaatgcgt ccgtagtagg aaagcggaca cgaaagtaat
    25321 caatcaataa gaatagtggt tcttgcggat tgaaacgctc aatctgtttt tctatgattt
    25381 ctcttaattc gtcattcaat ggctgttttc ctttttcaat gcggttgtaa tattcacggc
    25441 tgataccaca ggcaacagca agccttgttt gtgttactcc gtattcctca cgcttttgtc
    25501 ttagttcttc aataaagttt ttatcattca ttcttcaatt cctccaatca aaaaaggcaa
    25561 ctaccataat tgataattgc ctgtcattaa aaatctgtat agaaaaagca gtcaatccta
    25621 agactaactg ctttgtgtgt atggatataa aattgcttct aatcttgaat aggtatccaa
    25681 atttcaataa ctgaattatc actattccaa tgaaaacgat aatcgtattt ttcaaagtgt
    25741 aggataatat cttttatagg aatatacgat gtactaggaa taatttcttc ataaatcttg
    25801 ccataagttt catatatttt atccatagca ccaatatgct ccacaaccaa atatttataa
    25861 gacggtattt ctttatacag aaagccctct ggggtaatgg ttctttgggg aatagaacag
    25921 aaataataaa gttttccatt tcttctttcc ataaaagcat attttaccca atttcctgat
    25981 tgtgaaagat acgctttttt caaactactg ttaaactttc tccaaaactg tgtactaatc
    26041 tgaatattct ttttttgata attagtcagt tctacttcct gcccaataac tgcaaatgcg
    26101 tttaattcct ttatagaaaa atttatcatt ttaccttgtg agcagtgata acggtataac
    26161 tgtacgcatt taccgttata atacaattat caatcgttat ataccagttt tttccttttc
    26221 ttgtgatttc cgcattagaa aggctaattt tttctttgca ccattcaatt acattttcta
    26281 cattcaaaga taagttcttt ttaattctaa taacgccaag ttcagttgtg tgaagtcttc
    26341 ctaagttttc cagtaattca ttttccatat tttgatacct catttttcag caaatttatt
    26401 tgtatgtttt ataccccaat tataacattc tcatatcagc actacaataa aaataattaa
    26461 gcaccaataa tcttattgaa catctccaac cgtaaatctt tcacaccatc agtgataaac
    26521 actgagctga cagcaattac gacaactacc ataattgcct gcacttaaat ttctgcaaaa
    26581 gaaaacgaca atcaatttct tattttttga aatcaactgt ctttctcata tttactattc
    26641 aattttcata gtgatatagg tacaatttat atcactactg taaaaatggg gttttgcctt
    26701 ataaatacag cgttttccgc ttgttagtgt atagtgtttc ttgttttttg tgcccccctt
    26761 gttagataac gggggctttg aacggctcgc aagctcgccg aaccgccgaa gcgaaccgcc
    26821 cccttgcaag catggcggaa aaatcttaag gattttcccg ccaacgtggt cggatcgctc
    26881 cgtcggttta acagcttttc tgtttctctc tagttatcta cgcatataaa tcaaatcggt
    26941 tattccgtta taagtctgtg ttttacaaag ccttaacttt atttccctgt ctatgttgcc
    27001 caaaagacga accccatttc ctaacagagt aggaataaca gaaatgtaat atttatcaat
    27061 catatttttg cttaccaact gctgaacaag atttgctccg ccacaaatcc aaatatcctt
    27121 tcctgcttct tgttttagcc gttccaataa taaaacagga ttttcacttg taaaatgaat
    27181 ttgttgggaa gaattgcact ctttatgtgt taatacataa gttgtaaatt tattatatac
    27241 ccattcttgc ggagaaagtt cagtaacaac ctgatgagaa gtattccacc ccattaagac
    27301 agtatctata tctttggtaa attctgtata agtgtcgatg ttttcattgt catttccatg
    27361 accatttagc caagctactc caccattacc gtccgcaata tatccgtcaa ggctcatggc
    27421 aataaataaa actacttttc tcattttttc tcttttataa atacaaacag gaatttattt
    27481 ctctttttta ctcataaggg aacaactgat tgctacaaat aaacccatta aaatccatgg
    27541 aaatgctgct cttataaaat cactaaccat actttattcc tccttaaatt ccaatttcct
    27601 tagttctaca aactgaaatt tttgcctatg aatgtatctt gttctttata cgcaaacagg
    27661 cttctgtgtg cataatccaa tgtcttgaaa acggcatttg aattagtcca caaatatctt
    27721 tattacacca ataatcaatc agccaaattg ctttttcatt gtcgtttact acattcaacg
    27781 attctaagta tccttttctt tctttcggta tttttctttt caattcatca taagataacc
    27841 gttcaagcat tttctcagtg ctttcccata cttcgaaaat atataaatat aattcttcca
    27901 gattaagctg ttttgaaaag tctgcaatct gctgtttcat gagttcattt cctgttgtaa
    27961 tgataggtga attgatacgc tcttgataat tgcctgcaaa aaatacttgt tcatcttcat
    28021 ttatcactgt atgcacaact atatcttcaa tgcggaaaat atgccaaata gaataagcaa
    28081 tcgtcttact atgataacca tcagcgttta tgaaggggat tgagttaaaa tcttctctgc
    28141 ataattcttc tttgaaagat actaaggttt gtattaactg acttcgtaaa gtaagtaaag
    28201 tatcaatacc cctcttataa gtatctttct tttttatttg tgcctgcatt gttttattaa
    28261 gttcagacca ttccttattc atattcaatc ctctctcaaa ttctgatttt atcgccccat
    28321 acaaactgga atttgtctta caacaattta tatatgcata aaaagcaata tatttatgaa
    28381 aacaccctaa atccaaaatt tactcaattc attttttcct tacataaaat gttctcgtat
    28441 atttcacttc ctcaccaagt gtttccccta tcaaaacttc aaaatcattg atatcaaatt
    28501 ctggaaagaa tgtatctcca tcttcaatat ttaaatctac ttctgtgata tacattttat
    28561 ctactatttg caaagcttcc ttaaatagtc catatccacc agatatgtat acatctcgtc
    28621 ctttagccaa taataatgca tcttctaatg atttaactga aactaaatta tctccttgat
    28681 actctgttgt ggtggaaaca acaatattca ttctattagg caacggatga ccgatttctt
    28741 cataagactt tcgccccata ataaccacat tacccgttgt taactctcta aattgctttt
    28801 gttctccctt tattttccat ggtatattac cattcttgcc tataacatta ttctttgacc
    28861 tcgcaacaat caaacctatc atctatttca tcctccataa tatgatgtcg tacctgtgat
    28921 tccatcaagt gcaccacatc ttaacaaact caaaattatc tcgaactaac cagctcgaca
    28981 aattcctttt ttgtcttcat ctgcattata tcatattgtt ataagcattg ccatttcttt
    29041 tctgcttttg aaaagttact tatcagccta tgttttgtaa agggtgatat tcagcatttc
    29101 tcacgcaaca agcccttgac aaaccataga ctaataagtt ttaaggtaat caagcagaaa
    29161 tgaaatagtc tgctgtttca cggcaacaaa aaagagatac caacacactt atgtagtatc
    29221 tcttagtcta gtagcaatat tcttttgttt gcaagcaggt aaacatgggc tgtttcttaa
    29281 tatctttttg tttgggaaac acctttttcc catcctttca ccgttgccat ctctgctgcg
    29341 cacaatgcca cagggataaa ccatagcaaa ccgcctaata acaagaaaaa tactaagctg
    29401 aagcctgagg tcgcgaatgt gggatattca tataatgaaa cgaccattga gatggtcata
    29461 gccaaaaagc caaagaacgt taattttttt gcaggaattt tcgttttgct ttgttgcatg
    29521 aatcttcctc ttttctattt gaatttgttt caattgagtt tgttgccaaa gaagaaaaag
    29581 tccaatcctt cttcttgttc ttcttttgta gtagtgatcg ttgtaaagtg catcgccgat
    29641 tttaaaatca ctgttgtcag cacataggca tacacggcag taatgatgac accaatcact
    29701 tgaatcccta agaagtacca gctgcttgga ttcgttagta aatggctgtt agatgcaaag
    29761 atccctatca gaatcgtacc tgtgaatccg cccatgccat gaacacccca cacatcaagt
    29821 gcatcatccc attgtttctt tttacgaaaa tcaacagcag catgacagac gatacccgca
    29881 atgatcccaa tcagcatggc actttttgct tctacataac cggcacaagg cgtaatggct
    29941 gccaatccag caacggatcc tgttagtacg tcgacaaaat caaagccatt tccttttact
    30001 tttgcaatga tcagccaagt aatcatcgca aatgcaagag cgatgaatgt tgaaccaaat
    30061 gcattgactg cttgttcatc tgctcgaagt gcaccaccgg aattaaaacc gaaccagcca
    30121 aaccaaagca gtcctgtacc gatagcagct gccataagac tgctatgctg acttctttct
    30181 cctaaaggaa tctttctttg accaagtgtg aggatacatg ctaaagagcc gaatccagct
    30241 gtcgtgtgga tgactgctcc accagcgaaa tctctaaatc ccatctgatc caaaaatccg
    30301 ccaccccata tccagtggca aacagggaaa taaatcagca gattccaaaa aataacaagt
    30361 ataatatagc cttttaaatt caatctccct gcaaaagctc ctgtcatcaa aggaacggta
    30421 atcacgataa acatcagttg gtaaagaaag aacattaaaa atggaatcgt tgcaccatga
    30481 aacatgttgg ggaagcttgt aacatgtctc atcaaaaaga aatctgccgg gtttccaata
    30541 acaccgccga tatcccgtcc aaaggccaat ccgaatccac caaataccca cataatcgtt
    30601 gttatcccaa cggagagaaa agattgagac atgatcgtta gtatattatt cttccgtgct
    30661 aatccgccgt aaaaaaaagc tagaccagga gtcatcaggc aaaccattgc tgtacataaa
    30721 atcatgaatg ctgtatctcc tgtattgata ggtaatgtat tcattttttt cgcctccttt
    30781 ctacgattac gagtataaaa gaaggtgtag ttgttttttt cttctgtttt tttgaattaa
    30841 tacttgcatt tataaaagac agacaattaa taaaagcttg atgtgttgtg ctctttttcg
    30901 tttttcacac aaataaagac aaaaaataaa cggtcttctt tttatagaaa accgtttatt
    30961 ttcgtctttt tttattttta aagatatttt ttcataagga ataaagaata cttcatataa
    31021 aatatataaa aaagaaccgc agcgagtgtc gctacggttc tcaaatttgt tcttcatttc
    31081 aacataagtt tttcttcaat ttgcttataa tttacgtgca aagaatgaaa cgactaatac
    31141 taaaatgatt gcaccgatca atgatggaat gatagccatt cctgcaattg ttggtcccca
    31201 agttcctagt aaagcttgtc caattgctga acctactaaa ccagcaatga tgtttgcgat
    31261 gatccccatt gatgatcctt tattcgtgat tgctcctgca attgctccaa tgataccgcc
    31321 gacgattaat gcccaaataa atcccatttt aaattcctcc taactgactt cttacttctt
    31381 attgtacacg tggttcgtta ttttctttga ctttttgtgc acctttgcct acagcagatt
    31441 ttgctttatc tgtttggcgt gaagcaaatt gtcctgtgga ttttgctgct tctgttactt
    31501 tgtcttgtac tgtttcttga tctttttggt attcttcttg tgttttgatg tctgcaacat
    31561 tcacattcac ttcgataaca tctaaatgtg tcatattgtt tacttcagta ctgatcaatt
    31621 ctttgatttg gttgtaaata tcttcgatgt ctttaccgta ttctacgatc acatctagat
    31681 ccacagctac ttgtttctta cctacttctg tattgatacc tgctgttacg ttatctgtgt
    31741 tgactaattt ttcagccata tttgagaaaa atccgccatc taatgtcaat agaccatcta
    31801 catctttcaa tgcataaccg atgatttttt ggatgacttt gtcttcataa gtcaattcac
    31861 cattcatcat gcggttttct cctttttcat ttgccaaaac tgatccattt gttgtattca
    31921 tgtttttgtt ttccatcttt gtttcctcct atgatttaaa aaattgatca actattcctg
    31981 actgtttcaa ataaaaaccg attcctgcgc caattgctgt catgattatg atcaacaatg
    32041 tcttgaaaaa tccaatcgtt aaccataaaa cagctagcag tagtccaaga cccccaaaga
    32101 taatagggag cttgtatgct gaaaatagtt ctttcattat ttggccctcc tattccactc
    32161 gagagtgctt gtagtctctt gtatcttctt gttcttcata accgctgtat gtgactgcca
    32221 ctttgaccgt ttcttttgtt ccgagtactt tagttacttc ttcttcgata tcttgcatca
    32281 atacacctgt tttccctact aatgaagaag ttcgtttcaa gtctccgttg acatggatat
    32341 ggattcgatt tttcgttgaa cgtatccgta ctttaggtga atgaatgaac tcttcttcgt
    32401 gtaaatggga acgtaccaac ccttcgatcg cttttttgtc gattgttagt ttgccatctt
    32461 cccgtttcat aacgaaagtt cctcttgact ttggatagaa aagaacgact agcaagatga
    32521 taatagcaag aacggcaaat actacagctg cccagaaaat atattgttgc atgtagtaat
    32581 tcgttaaagt aaagaagcga aatcgttcca attgttcagg cataatatac ggcgcattga
    32641 tcaacgcaat cgctagaaat actgaaagta gcagtaaact catgataaca ccgattattt
    32701 ttaacccctt attcattttc cacctcctat aaacttattt ttaccctgta aataactcta
    32761 ccgacaatca cagtatagat agcgaacagt tagcatagca tcagtaaata aagcgctacc
    32821 aaacattgct ggatcataac ggcgttttat tgccagtctg atactgaaat aacaattgct
    32881 tacttattct ctatttactt ttgattgctg gctatactta aaggatatta ttcaagatta
    32941 aaaattgcaa agattatgct cagttattat tacaagattc tatttat
//
LOCUS       DAEGIQ010000023        32438 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000023 DAEGIQ010000000
VERSION     DAEGIQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.023"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            151..2748
                     /gene="adhE"
                     /locus_tag="KMT45_000958"
                     /gene_synonym="adhC"
     CDS             151..2748
                     /gene="adhE"
                     /locus_tag="KMT45_000958"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBE7004435.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2977..4083
                     /locus_tag="KMT45_000959"
     CDS             2977..4083
                     /locus_tag="KMT45_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBE7004436.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4100..5128
                     /locus_tag="KMT45_000960"
     CDS             4100..5128
                     /locus_tag="KMT45_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBE7004437.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5134..5613
                     /locus_tag="KMT45_000961"
     CDS             5134..5613
                     /locus_tag="KMT45_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBE7004438.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5629..6924
                     /locus_tag="KMT45_000962"
     CDS             5629..6924
                     /locus_tag="KMT45_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBE7004439.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6935..7759
                     /locus_tag="KMT45_000963"
     CDS             6935..7759
                     /locus_tag="KMT45_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBE7004440.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7924..8823
                     /locus_tag="KMT45_000964"
     CDS             7924..8823
                     /locus_tag="KMT45_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7004441.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8949..9818)
                     /locus_tag="KMT45_000965"
     CDS             complement(8949..9818)
                     /locus_tag="KMT45_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBE7004442.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9971..11431
                     /gene="rhaB"
                     /locus_tag="KMT45_000966"
     CDS             9971..11431
                     /gene="rhaB"
                     /locus_tag="KMT45_000966"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBE7004443.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11428..12684
                     /gene="rhaA"
                     /locus_tag="KMT45_000967"
     CDS             11428..12684
                     /gene="rhaA"
                     /locus_tag="KMT45_000967"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBE7004444.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12698..13543
                     /gene="rhaD"
                     /locus_tag="KMT45_000968"
     CDS             12698..13543
                     /gene="rhaD"
                     /locus_tag="KMT45_000968"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBE7004445.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13545..13856
                     /gene="rhaM"
                     /locus_tag="KMT45_000969"
     CDS             13545..13856
                     /gene="rhaM"
                     /locus_tag="KMT45_000969"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBE7004446.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13938..15089
                     /gene="fucO"
                     /locus_tag="KMT45_000970"
     CDS             13938..15089
                     /gene="fucO"
                     /locus_tag="KMT45_000970"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBE7004447.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15216..15974
                     /locus_tag="KMT45_000971"
     CDS             15216..15974
                     /locus_tag="KMT45_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBE7004448.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16074..16718
                     /locus_tag="KMT45_000972"
     CDS             16074..16718
                     /locus_tag="KMT45_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBE7004449.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16725..17732
                     /locus_tag="KMT45_000973"
     CDS             16725..17732
                     /locus_tag="KMT45_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBE7004450.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17748..18590
                     /gene="kduI"
                     /locus_tag="KMT45_000974"
     CDS             17748..18590
                     /gene="kduI"
                     /locus_tag="KMT45_000974"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBE7004451.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18610..19413
                     /locus_tag="KMT45_000975"
     CDS             18610..19413
                     /locus_tag="KMT45_000975"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBE7004452.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19662..20726)
                     /locus_tag="KMT45_000976"
     CDS             complement(19662..20726)
                     /locus_tag="KMT45_000976"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBE7004453.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20723..21808)
                     /locus_tag="KMT45_000977"
     CDS             complement(20723..21808)
                     /locus_tag="KMT45_000977"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBE7004454.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21821..22798)
                     /gene="mvaD"
                     /locus_tag="KMT45_000978"
     CDS             complement(21821..22798)
                     /gene="mvaD"
                     /locus_tag="KMT45_000978"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBE7004455.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22791..23735)
                     /gene="mvk"
                     /locus_tag="KMT45_000979"
     CDS             complement(22791..23735)
                     /gene="mvk"
                     /locus_tag="KMT45_000979"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBE7004456.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24051..24710
                     /locus_tag="KMT45_000980"
     CDS             24051..24710
                     /locus_tag="KMT45_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBE7004457.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24795..25700
                     /locus_tag="KMT45_000981"
     CDS             24795..25700
                     /locus_tag="KMT45_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBE7004458.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25702..26334)
                     /locus_tag="KMT45_000982"
     CDS             complement(25702..26334)
                     /locus_tag="KMT45_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE7004459.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26485..26610
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26654..27178
                     /gene="infC"
                     /locus_tag="KMT45_000983"
     CDS             26654..27178
                     /gene="infC"
                     /locus_tag="KMT45_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBE7004460.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27250..27450
                     /gene="rpmI"
                     /locus_tag="KMT45_000984"
     CDS             27250..27450
                     /gene="rpmI"
                     /locus_tag="KMT45_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBE7004461.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27503..27862
                     /gene="rplT"
                     /locus_tag="KMT45_000985"
     CDS             27503..27862
                     /gene="rplT"
                     /locus_tag="KMT45_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBE7004462.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28114..29451
                     /locus_tag="KMT45_000986"
     CDS             28114..29451
                     /locus_tag="KMT45_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBE7004463.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29499..31628
                     /locus_tag="KMT45_000987"
     CDS             29499..31628
                     /locus_tag="KMT45_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBE7004464.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31603..32292
                     /locus_tag="KMT45_000988"
     CDS             31603..32292
                     /locus_tag="KMT45_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004465.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT        10292 a         5838 c         7140 g         9168 t
ORIGIN      
        1 tctatacaaa cacctgtaaa taaagcaatt ttatatttaa aaataactgt gaaaaaaatc
       61 acaaaaaagt gtttacatag aaaaataaat attgtatgat atacatgtga attatttaac
      121 aaataaccat gacccattag gaggataatg atggctaaag taatagaaaa agaaaaaacg
      181 aagacaattg acgtacaagc aatgattgat gatttggcag aaaaagcaaa tgcagcattg
      241 aaagaaatgg aaagctttga tcaagaaaaa gtagaccata tcgtgcatga aatggcaatg
      301 gcagctttgg atcaacatat gccattagcg aagatggctg tagaggaaac aggccgcggt
      361 gtttacgaag ataaagcaat caaaaatatg tatgcgtccg aatatatttg gcataatatc
      421 aaacatgata aaacagttgg tgtgatcaat gaagatgtcc aaaaagggtt gattgaaatt
      481 gctgaaccag tcggtgtggt ttgcggagta actccaacaa cgaatccgac ttccacaacg
      541 attttcaaat caatgatcgc gttgaaaaca agaaatccaa tcgtttttgc tttccatcca
      601 agtgcccaaa aatcttctgc agaagcagca cgagtggtac gtgatgctgc tatcgctgcc
      661 ggagctcctg aaaattgtat ccaatggatc gaacatcctt cgatcgaagc gacttctatg
      721 ctaatgaacc atccaggtat cgcgatcgtc ttagcgacag gtggagctgg aatggtcaag
      781 tctgcttact ctacaggaaa accagcttta ggtgtaggtc caggtaacgt accagcttat
      841 attgaaaaaa cagcaaaaat taaacgtgca gtgaacgatt tgatcgtttc gaaaacattt
      901 gataatggaa tgatttgtgc ttctgaacaa gctgtgatcg tggataaaga aatctatgca
      961 gcagtaaaag cagaattcca agcacaccaa gtttatatcg taaaaccaga tgaattgcaa
     1021 aaattagaag atgcagtgat gaacgaagga aaatatgcgg tcaatccatc aatcgttgga
     1081 cattctgcga tggaaatcgc aaaattggca ggaatcagtg taccaaaagg aacgaaaatg
     1141 ctgattgctg aattggaagg cgtaggccca gattatccgc tatcaagaga aaaactttct
     1201 ccagttcttg ccatgatcaa agctaacaac acggaccatg cgtttgattt gtgtgaaggt
     1261 atgttgaatc ttggaggatt aggacataca gcagttatcc attcagaaaa cgaagagttg
     1321 catgtcaaat tcggtcttcg aatgaaagct tgtcgtattt tagtgaatac accatctgca
     1381 gaaggcggca tcggtgatat ctataacgaa atgattccgt cattgacttt gggatgcggt
     1441 tcttatggta agaactctgt ttctcgtaac gtctctgctg taaacttaat caatgtcaaa
     1501 actgtagcga aacggagaaa taacatgcaa tggttcaaat taccgccaaa aatcttcttt
     1561 gaaaagaact ctctattgta cttagagaaa atggaaaacg ttgaacgtgt catgatcgtt
     1621 tgtgaccctg gtatggtcca attcggttac tgcgatacag ttcgtgaagt attggcacga
     1681 cgtaaaaatg atgtgaagat cgaagtcttc tcagatgttg aaccgaaccc atcgacaaat
     1741 accgtatacg caggaacgaa actgatggca gacttcaagc cagatacagt aatcgctctt
     1801 ggaggcggtt ctgcaatgga cgcagctaag ggaatgtgga tgttctatga acacccagac
     1861 acatctttct ttggtgcaaa acaaaaattc ttggatatcc gtaaacggac atataagatc
     1921 gacaaaccag aaaaaacaca attcgtatgt atcccgacaa catcagggac tggttctgaa
     1981 gtaacgccat ttgcggtcat cactgacagt gaaacacatg tgaaatatcc gttagcagat
     2041 tatgcattga cgccagatgt cgcaatcgtc gatcctcaat tcgtcatgtc agttccagca
     2101 tctgtcacag cagataccgg gatggatgtg ttaacacatg caatcgaatc ctatgtttca
     2161 gtcatggctt cagattatac acgtggattg agcctacaag caatcaagtt agtctttgac
     2221 tatttagaaa aatcagtaaa aacacctgat atggaatcac gtgaaaaaat gcacaacgct
     2281 tctacaatgg ctggtatggc gtttgctaac gcattcctag gtatttgtca ctcagtcgca
     2341 cataaaatcg gcggggaata cgggatccct catggacgga caaatgcgat tcttttaccg
     2401 catattatcc gctataatgc aaaagatcca caaaaacatg cgatgttccc taaatatgac
     2461 tatttccgtg cagatacaga ttacgcagat atcgcaaaat tcttaggtct gaaaggcgaa
     2521 acaacagagg aattagtcga agctttagca acggctgttt atgaattagg aaaatctgtc
     2581 ggcatcaaca tgagtctaaa agctcaagga gtcacacaag aaacactaga tacaaccgta
     2641 gaccgtatgg ctgaattagc ttacgaagac caatgtacga cagctaatcc gaaagaacca
     2701 ttgatctctg aattgaaaca gatcatcatt gatgcttaca acggataagc aaaagaaagc
     2761 gtagaaaaaa gataagaaaa tagatgaaaa agaacttgga aaacgttgtt tctccaagtt
     2821 ctttttttaa tttccaagta actgaaagtt acttttgtta tttaacattt aggtcaattt
     2881 tagacagttt ttgttcaaaa agcgatattt atagtggacg gtattactgc tatattaagg
     2941 atataaaatg aaagcgttta aaaaggtggc gtaaagatga ttattaaaga agagattttt
     3001 gtacctaaag aattagtgaa agaaaaaatt gacctgctcg ttgaaaacct gacaacgatc
     3061 aaagacgata atggtgaatt tttgttggac tttgatggac tgaaagtaga tgacaaaagc
     3121 tggacagtgt ggaattggcc acaaggtgtc ggtctatatg gtatttataa aaattaccag
     3181 atgacaaaaa atcctcgcgc ttatcaagtg gtcaatgaat ggtttgagga tcgtatggaa
     3241 gaaggcgctc cgcccaaaaa cgtcaataca atggcaccgc tattgactat ggcttacttg
     3301 tatgaagaca cgaaagattc acgctatttg ccttatttag aacaatgggc agaatgggtc
     3361 atgaatgaca tgcctcggac aaatgaagac gggttacagc atgccacata tggaccggaa
     3421 aataagaatc agttgtggga cgatacattg atgatgacag tgcttccttt ggcaaaaatc
     3481 gggaaactgt tgaatcgccc agaatattta gaagaagctc gttaccagtt tatgatccat
     3541 atcaaatact tgatggataa acgaacagga ttgtggtatc acggctggac gtttgaagga
     3601 aatcataact atgccgaagc attttgggct cgtggaaact gctggttgac gattgctatt
     3661 ccagagatca ttgaaatctt agaattatcg aaagaagatg cattgcgcaa attattgatc
     3721 gaaacacttg aagcacaagt gcgtgcatta aaaacttatc agtctgaaag cggtttatgg
     3781 catacgttgt tggatgatcc ttcttcttac gtagaatctt ctgcaactgc aggatttgct
     3841 tacggtattt tgaaagcggt ccataaacgt tatttaccac aagaatataa agaagtcgct
     3901 tatcgtgcta tccaaggatt gcttgaacaa atcgatgaaa aaggagaagt tcaaaacgtt
     3961 tcaatcggga caggaatggg ggacagttta gatttttatc ggaatatcgg aatcacagca
     4021 atgccttatg gacagtcgtt aacggtgctt tgtttgacag aattgctcgt ctcttattgt
     4081 taaaagaaag gaaacccata tgaaaaaaac atcactgctg tttgcagcaa gttcgcttag
     4141 cctaggtctg ctaggtctaa gcggctgcgg aaataattct agtgtggcaa ctgtcgcaga
     4201 tgcagatgta aatgagatag ttaccttgcg ctttgcttat gcaagcaaca gccagccagt
     4261 aattgatgca atgaatgaat ttgggcgttt agtaaaagaa aaaacagatg gttccgtaca
     4321 gatccagtat ttcccagatg gacaactggg tggagaacgt gaactgatcg aattgacaca
     4381 gtcaggagca gtagatttca caaaagtaag tgcctcagcg ttggaaagtt tctctaaaga
     4441 ttattcgatt ttttcagttc cttatctatt caatgatgaa gaacatttct ttaaggtgat
     4501 ggataatgaa gaaataatgg agccaatcta ccaatcgacc aaaaatttag gatttaccgg
     4561 attgacctat tatgattctg gtcagcgcag cttttatatg gtagacggtc cgatccatac
     4621 gccggaagat ttgaagggaa agaagatccg tgtgatgcaa agcgaaaccg ctatcaaaat
     4681 ggtagagctt ttaggcggtt cgcccgtacc tatgggaagt gatgaagtgt acacgtctct
     4741 tcaatccaat ttgatcaacg gatcagaaaa caatgaattt gtcttgtata cagctggaca
     4801 tggtggtgtg gctaaatact attcctatga tgagcataca cgtgtgccag atatcatcat
     4861 catgaatgat gccatcaaag aacagctaac tgatgagcag gaaaaagcca ttgaagaagc
     4921 agcgaaagaa tcaacggttt ttgaaattga agcttttgca aaagcaattg aagaagaaaa
     4981 agctttagca caagaagaac atggagtcca attcaatgat gtagataata caccgtttcg
     5041 agaggcagtt gccccacttc atgatatatt caaaaacgat cctacctaca aagaccttta
     5101 tcaaaagatc caagagcaag gagcgtagct gtgatgaaaa tgattttaaa caaggtatta
     5161 gagatcctgg gagctgtttt gcttgttggg atggtcgctg ttgttttatg gcaagtattt
     5221 tccagaacgg ttttaggaaa tccaaatacg gtcacggaag agcttgttcg attcggtctt
     5281 gtctggtttg ccatgttgtc ttccgcgtat gttgtgggac aaaaagcaca tttagccgta
     5341 acggttttaa gtgaaaaatt aactggcaaa aaaagtaaaa ttttagaagt tgttgttcaa
     5401 agtttatttc tggtatttgc tgcgatgatc atggtgtatg gcggatggaa tgctgtcaca
     5461 ttgacaatgg ggcagatctc gccttcttta agtattccta tgggatatgt ttatctgtca
     5521 gtacctgtat cgggggtact catcattatc tatagcctga tcaatctaat cgatacatta
     5581 agcaacaagc cagcaattca agaaaaaata taaaaatgga ggaataaaat ggcaatagaa
     5641 gccggattag tgttattttt tgtatttgtt gtgttgcttg tattagggat gccgattgct
     5701 ataagtattg cattctcatc gatgatgact ttattgttag ttataccgtt tgatgtttct
     5761 gcttttagtt cagctcaaaa aatggtggga agtatcaata gtttttcgct gatcgctatt
     5821 ccgtttttcg ttctctcggg cattatcatg aataatggag ggatagctaa gaaactagtg
     5881 gattttgcga tgttgttcgt cgggcgcgtg ccgggggctt tggctcatac gaatgtgtta
     5941 gggaatgctt tatttggttc catctctagc tcggccattg ccgcttctac cgcgattggc
     6001 ggagtgttga tccctcagca agtggaagaa ggatatgacc ggaagtttgc gacagcggtc
     6061 aatatcgcgt cagctccgac aggtatggtc attcctccaa gtactgcttt tatcatgtat
     6121 tcacttgttg ctggtggtgc ttcgatctct gctttgttta tgggaggtta tctcgtaggc
     6181 gctttgtggg cgctaggaat catgctagta gcattcatcg ttgctaaaaa gaataaatac
     6241 cctgttgtcg aaaaaggaca gatggctcat gctggaaaaa taataaaaga ggcgattcct
     6301 agccttctat tgattgtcat cattatcggt ggtatcttga cggggatatt tacagcagta
     6361 gaagcttcag cgatagctgt catttactcc ctgttgattg ctatgtttta ctataaaacg
     6421 gtaaaactaa aagatatacc taatatgttg tttcaatcag tagcaatgtc agggacgatc
     6481 atgttcttat tggctacttc ttccatgatg tctttcgcca tggcgtttac cggtattcct
     6541 caggcaatta gttcgttgat tatgggcgta acggataata aatttattat tctgttatta
     6601 gtcaatcttg tgttattact ggtgggtatg tttatggatg tggcgcctgc tatcttgatt
     6661 tttacaccaa tatttttacc aatcgtcaca agtgtagggg ttgatcctgt acattacggg
     6721 ttgttttcaa tcatgaatct gtgtgtaggc tccatcacgc cgccagtagg tacaggacta
     6781 tatgtcggtg caagtgtagg aggcgtaaaa gcagaggcaa tgctgaaacc gcttcttcct
     6841 ttttatgggg ttatcttagt cgtcttgttt ttgattactt acttcccgca aatcgtcatg
     6901 tggctaccca atatgctgag ttaggagaat agttatgttg aacattacaa ttagaggaca
     6961 tgatctttcc gaagtccaaa cgatagaaga tcttgcagaa aaaacaaaag aacaagggat
     7021 tcatacactg cagctcgcct taggtctttc gtttcctgaa ataccttctg gcgcaaatga
     7081 gatcaattct gggatgggaa attatgtcaa acgagtttta gaaaagcaag aagtgaccgt
     7141 aggaatttta agttgctata tcaatatgat tcatccagat ttaacgatac gacaagagtt
     7201 gctgacaaag ttcgagcagt atcttcgcta tgcttcttcc tttggtgcgt cgatggttgc
     7261 ttcggaaact ggatgtgttt tgccggaaat ccaatatacg gaagaaaatt tcacagatga
     7321 agcatttgct gaagctgtgt ccgttatccg tcgcttggta aaagctggag aaaaatacca
     7381 aatgatggtg gggatagaac caggtttgaa tcatccggtg tattctttag caagggtaga
     7441 gcagctgatc caagcagtag attcggacta tctaggaatc attttagatc cgaccaattt
     7501 gatcacaagt agtgattatc aagaacaagt ccaattggta gaagaagctt ttgaacgttt
     7561 tggtgaaaag atatgtgcgg ttcatttgaa agattttcgt gtcgaacagg aaaaaatcgt
     7621 gccggtaaat ttgggagacg gtgtgatcga gtacacaaaa atcaaagaaa tcatcatgaa
     7681 gaatcgtccg tacctgtatg tggttcttga agaaacaaaa gatgatggga ttcgttatgg
     7741 aagaagtctt cttgaataat gtaaagaaga cgttggttaa ctaatattta gaatggaggg
     7801 ggcggggcta gaagatgcca cgtctcttct tttttataag agtatataaa agcagctctt
     7861 tttgattaca aaaaacgtgg tataataaaa ttgtgcataa gtgaacaaaa ggaggtgcgg
     7921 cacatgctcc aaaagaaaat cagcggtgaa caagtcaata cgatggtttc tgatcagtat
     7981 gaaatctatc atgtcaaaga tgtcgtcagc caaaataaaa cgatctatca tcatcatgat
     8041 ttctatgaga tccatgctac attaaaggga gaagcgactt tttatttgaa cggacgacaa
     8101 tttacgatca aagcaggaag tgtgctgctc atccattcca atgatttgca tcgtatcgtc
     8161 cgccaatctt cagatgtatt cgagcgtgtc tatatctttg tcaccccttc ttttctagaa
     8221 agtcgttcca cgccatggtc taatctatcg gcttgttttc aaccaatggg agagaagagg
     8281 agtcgtgtat tgcaaataga acaggaagta ttgaagcaaa aattatcatt tatcgaccat
     8341 cctcctgata aaaaaattta cggtgaagac ataaaatatg aacaagctct ggtagaatac
     8401 ttgatttttc tcaatcaagt tgtacaaaaa gaagagagtg cgcaagaaca gaatgctgta
     8461 gttcctaatg agcgtgtgga acaaatgtta caatatatct caaaaaattt atctcatccg
     8521 ctttctttag aggaaatgga aaagcagttt tttgtcagca aataccatat cacacgagaa
     8581 tttaaaaagt acactggatt tacgtttcat cagtatgtaa tgaagaaaaa gctgatctat
     8641 gccaagcact tgttgcgaga ataccgcagc tctagtgctg tatatagtaa aagtggtttt
     8701 tcttcttatc cgcattttct gaaagcattc aagagagagt tcggcataac gcctaaagaa
     8761 tttttgaaac gtgatacaca gaaacagttg gttcattatg aacattttga agatgaacaa
     8821 tgagaatgat cactaaaaaa taaccgcagt agctgctgtt gtttacatag aagcagaaac
     8881 tgcggttatt ttatttttat ctatatattt caaaatattt tgaaaaaatg acgtacatat
     8941 tatatgaatc agctgaattt acgaaagtct ctcggcgata gtcctgtttt tgctttgaac
     9001 aggcggctaa agtaagcaaa attctcaatc cctatttctt cggaaatcat tcgaaccgta
     9061 aattctgtat accgaagaag gtctttagcg taagaaagtt ttttgctgat caaataatcg
     9121 attggtggag aaccgataaa tttggaaaaa tctttgctca gctgatattt attcaataag
     9181 aattgttgtt ctaattgatc aagtgtaatc gtttctttga aatgttcgtc taagtattgc
     9241 ttcagtttgt aaacgtaggc gggaatatcc tcttcttcga aatctagctg atacttttgc
     9301 aaaatcagtt cattcagcaa ctcgtggata gctacagagg actggaaatc ggtacgggca
     9361 ttcgtgcctt gttgcatgtg gatcaattct gtgatcaaat ggtggatctt gttttctgat
     9421 ggatctcctg ttgaatggaa ggtgttatgg ccgtcttttg tgaactcctg ataaaatgat
     9481 tggacgttct gaccattcaa atggatccaa tccatttccc atggttcttc gctgatcgtt
     9541 tgataacgct gatattcttg gcagtcaata aaaaataagt ctccagcttt tagctgttgc
     9601 tgattatcat tatatgtaag cgaacctttg ccagacaaag taaactttat caagtaagaa
     9661 ggtaaatgtg ctcgttcagt atagtaaggg attggtgctt tgaagtaacc gatttcttgg
     9721 atataaaaaa ataaattcct gcttctttgt gtaggcgtca agatcactct ttttgagtct
     9781 tccgaccatg agtggttggc tttttcccag taatccatgt aaacactccc aacttgtcaa
     9841 gataatgcaa ttatatacca aaatagtgtg aagcaaaata taagattctg tattatgata
     9901 gcattgtcaa taaatgataa cgctttatta ataaagtgct aatcaacaat aaagtaaaag
     9961 aggtggagaa atgaatccaa catttgttgc catcgatatc ggtgcatcaa gcggacgact
    10021 aatgaaaagt acgagggatg caaaaggttc tttgatcttg gaagaagtac accggttcaa
    10081 aaacggcttt cggaacaaag acggtcacga tcgctggaag atcgattatc ttatcaaaga
    10141 gattttgatt ggatttgaaa aattaaagca gacagggatt gatcgccttt ttgcggggat
    10201 cgatacatgg gcagtcgatt actgtctgat tgatgaacaa ggggatcgga taaatgatcc
    10261 gattgcttat cgggacaagc gtaccaatgg agcagtagaa cagttttctg aaatctattc
    10321 tctagataag ttgtatgagc agacagggat tcagatccag cctttcaata ccttgttcca
    10381 attgtttgct gaagaaaaag aaaatttggc aaaagcacat aaattgttac tgatacctga
    10441 ttatttaggg tatgtcttta ctggtaacat ggtgacggag aaaactaatg catctactat
    10501 gcagctgctg aatgcaggga caaaagaatg ggaaatagaa cttcttgaaa aaatcggtgt
    10561 ctcgaaggaa ttatttccac cattgacaga gccgggaacg atactaggag atctgcaaaa
    10621 agaacaattt tcaaaatatg atttgccaga tgtgaccttt atcacagtag caagtcatga
    10681 tacggcgtca gctgtattag gaacgccagg tcaaggagaa cgttggggtt atatcagcag
    10741 tgggacatgg tcgcttttag ggattgaaac aaagatcgcc aatgtttctc gcaatgcttt
    10801 ttctgaaaat tatacgaatg aatggggtgc ccataacacg gtgcgttttt tgaaaaacat
    10861 catggggatg tggttgatcc aggaagttgc ccgaatgcaa gagtacgagt acagttatgc
    10921 ggaattggca gagcttgctg caaaagaaaa accgctgcag caagtgatgg aaatcaatga
    10981 ccctcgattt ttgaatcccc caaatatgat agaagagatt cagcaatact gttttgaaac
    11041 agagcagcca gtccctgtct catcgggagc tatcgctcgt tgtgtgtatg ataatcttgc
    11101 tctttgttac gcacaagagc tgatcaaagt agaacactta actggaacga aattggaaaa
    11161 gctgcacatc gtagggggcg gatcgaataa tcgtctactg aatcaattga cagcagatta
    11221 tgccggaatc attgtagaag caggacctag tgaagccaca gcaatcggga atgttgccat
    11281 gcaaatgctc agtacaggtc agttttcgaa tatagaacaa gcaagaaatg taatcaaaca
    11341 gtcgtttcca tgtgaaactt tttacccaga tgaaaataag caaaacacga tggaatggta
    11401 cgtgaataaa atcaaaggag taatcgtatg aacacgattg aaagaaatta cgaacaagca
    11461 aaagaaaaat atgcaactat cggtgtagat acagatgctg tactagaaaa gatacaggac
    11521 atcaaaattt ctatgcattg ctggcaagga gatgatgtca aaggtttctt gacaccggat
    11581 ggcgaattga ccggtggaat catggcgaca gggaattttc caggagccgc acgaacacct
    11641 gaagagctta gacaagatct ggagaaggct tattcgttga taccgggcaa acacaaattg
    11701 aaccttcatg ctatctattt ggatacagaa gaatctgtcg atctaaatga aattgagccg
    11761 aaacattttg aaaaatgggt agagtgggca aaaaaagaag aaatcggtct tgacttcaat
    11821 ccgactttct tttcgcatcc aatgatgaaa gacggcttta cattggctca tccagataaa
    11881 gaagttcgtg aatactggat cgaacacggg aaaagaagcc gcaaaatcgc ggagtacttt
    11941 gggaaagaaa ctgggcaaac aagtatcaat aacttttggg tacctgatgg gatgaaagat
    12001 aatccgattg atcgttttac cccacgggaa cgtttaatgg aatcactcga tgaaattttt
    12061 acagaagagt tagatgaacg gtatgtacaa gatgcagtag aaagcaaact atttggttta
    12121 ggcgctgaag cttatacagt cggttcccat gaattttata tgggttacgg gttgactcgt
    12181 gacaaacttg tttgtctaga tgctggacat tttcatccaa cagaagtgat ttccaataaa
    12241 ttatcttcat tagctttatt cagcaaagga atcatgttgc atgtttcccg tccagtgcgt
    12301 tgggattcag accatgtggt tttgatggat gatgaattgc aggaaatcgc taaagaatta
    12361 gtccgtaacg aattgttgga aaaaacaaat atcggtctgg atttctttga tgcaacgatc
    12421 aaccgtatcg ctgcttgggt catcggtacg agaaatacac aaaaagcact gctgaaagct
    12481 atgctggaac cagttgagcg attgaaagaa atggagctgg cttttgactt tacttcaaga
    12541 atggcttata cggaagaatt gaaagatttc ccgtatgccg atgtatggaa ttatttctgt
    12601 tacaaaaatc aagttccagt tggtttggat tggctggaag aagtccagga atatcaaaaa
    12661 gacgtgttgg aattaagaaa ataggagtgt ggctatattg aaaaaaatag atattttaca
    12721 agcgccttat gtgaaagaaa tgatcgaaac gacgtcgaat ctttatcgct tgggatggga
    12781 tgaaagaaat ggcgggaata tttcttattt actaaaagaa gaagagatca caccgttctt
    12841 ggatcctgaa aaagtgatcc gccgcatctc gatgattttt gatgccagcc gtttagcagg
    12901 atgctatttt attgtaactg gttcaggaag atattttaaa aatgtagcga gcgaacctgc
    12961 cgagaatctt ggaatcgttc gagttgctga agatggcaaa acactggaac ttttgtgggg
    13021 gctggaaaac gaatgccttc ctacaagcga gttgccaagt catttcatga gtcatatcgc
    13081 ccgtttagaa gtagacccag ataatcggat cgttatgcat tgccatgcta gtcatctatt
    13141 agcgatgagt tttacgcatg aattagacga acgttccttt agtcgtacgc tatggcaaat
    13201 gtgtacggaa tgcttagttg tttttccaga aggaatttcc atcattccgt ggatggttcc
    13261 gggaacaaat gagattgggg aagcaacagc tgaaaaaatg aaagaaactc gattggtttt
    13321 atggccacaa catggaatct acgggtcagg aaaagacatg gatgaagtct ttggtttgat
    13381 cgagacagca gaaaaggcag cagaagtcta tacgtacgtc aaagcgcaag gaccaatctt
    13441 gcaaacaatc acggacgaaa acctgtggca tttagcagat gcgtttggtg tcactccaaa
    13501 agcaggttat ttggaggaag ttcatacgaa agcaggtgtc tgacatggtg aaaaaagctt
    13561 tttgcatgaa agtttaccca gaaaaacaag aagaatacaa aaaaagacat gatgaacttt
    13621 ggccagaaat gcgtacagca cttcgtgaac acggggtcat cgactattcg atctatcttg
    13681 atccgagtac aagtttgtta tttggttact tagtgatcga agatgaaaag ttatgggaaa
    13741 aaatggcaaa tacatctatc aatcaaaaat ggtggaagta catggaagat atcatggaga
    13801 caaatccaga tgcctctcca gtcagttggg aattgatgcc agtctttgaa ctgtagcaac
    13861 aagcaactgg gcaaccggtt gcttggggtt ctttcgtttt tattgtgaaa aataatacaa
    13921 attaggagga atagaaaatg agtaatcgaa tgattttaaa tgaaacatcg tattttggaa
    13981 aaggagcgat tgagtcgatc gtccctgaat ttcagcagcg aggattcggc aaagcagctg
    14041 tcattactga caaaggattg atcgagcacg gtatagcaac aaaagtaaca gaacttctag
    14101 ataaagcagc aatcgcttat gctttatacg acgggatcgt acctaatcca acgatccaaa
    14161 atgtaaaaga tggagtggct tttgttaaag aggcagaggc agactgtttg atcgcaatcg
    14221 gtggaggttc gccaatcgat acggcaaaag ccattgggat tattttgacg aatccagagt
    14281 tttcagacgt cattagttta gaaggtgttg ctgatacgaa aaatccatgt ttacctatat
    14341 tagctatacc aacgacttct ggaacagcag cagaagtaac gatcaactac gtcatcacag
    14401 atgaagtaaa tcaccgaaaa tttgtttgtg ttgatcctca tgatatccca atcgtcgcgt
    14461 tcatcgacag cgatatgatg atgggaatgc cgaaaaaatt ggctgcatca acaggtatgg
    14521 atgctatgac gcatgctatt gaagggttta tcacaaaagg cgcttgggaa atgactgata
    14581 tgctgcattt aaaagcaatc gaaatcatcg gccattcttt ggaagcttct gtagatggcg
    14641 atcaaaatgg gcgagaaaaa atggcattag gacagtatat tgcgggaatg ggcttctcaa
    14701 atgtcggttt aggactggta cacgggatgg ctcacccgtt aagtgcatgg tacaacattc
    14761 ctcatggcgt agcgtgtgca gcacttttgc caactgtcat gaaatataac aaagaatata
    14821 ctggagaaaa gtaccgtgag atcgctcttg ttctagggat taaaggagca gctgaaatgt
    14881 ctttagaaga tgtaagagaa gcggcttgtg gtgagatcga tcggttgagc aaagcggttg
    14941 gaatcccaga aaccatttct gaattaggtg taaaagaagc agatattcca gcaattgcag
    15001 aagatgcttt gcgagatgta tgcacaccag gaaatccaag agaaacgaca gtagaagaaa
    15061 tcattgcttt atatcaaagt ttgatgtaaa aacgaagcgg atcttcaaca ttaaattgcc
    15121 tgtaaagaga aatggtagtt tatatctgga tatctctttt aaattgatgg tatttccgct
    15181 ataatttttc ttataaagaa ataggagaat attatatgga aaataaaaag ccttatggaa
    15241 ctgtattgat caaagcctct aaaatactag actatttagc ggaaaaccaa gatgtctctt
    15301 tgcagacgat cgcaaagggt gtgggaatga cctcttctac agtcttgaaa atactagata
    15361 ctttgttgat gatcggctac gtaaataaaa attcagagaa aaactatcgt ttaggtgcaa
    15421 agcttattcg ctacgcgaat aaaaacattg aacaaatcga tttggtagag ttgactttac
    15481 cgtttttaga acagctgcag caaaagatcg atgagactat ccacttaggg gtattggaca
    15541 acaatgagat cttgtacgtg aacaaactag atccaaaaca tcaaacgatc cgcatgtctt
    15601 caaaaatcgg aatcacccga ccattataca attctgcgat gggcaaagcc gtattagcag
    15661 aattcagtga agagcagtac actgattatc ttgaaaagca tccgttgatc ccttacacag
    15721 aatatacgat caccaatgca ttacggttga aaaaggaaat caaaacggta aaagaaaccc
    15781 acatcgcatt tgacgatgaa gaaatcgaac gtgatatttt ttgtatagga gcctcaatcg
    15841 tgaaaaacga acaaatcatt ggtgcattta gtgtaagtat gccgaaatat cgtttaacag
    15901 atgagacaaa agaacaaata atcagtgccc tcaagcaaac aaaaagcgaa atcgagcaaa
    15961 gaattcaaaa ataatgaaaa aagcgtttgc aatttttctt ttctgccgta taatatgggt
    16021 atagatttcc aagttataaa ataaaatttc taaataggaa ataggtggag aagatgaaaa
    16081 aaattgatat tttatctcga ttgaaaaacg ccggagtgat tgcggtggta cgcggaaaaa
    16141 gcaaagaaga agctttgaat gcttgccacg caatcatcaa aggtggtcta actggtattg
    16201 agttgacttt tacagtccct caagcagatc aagtaatcaa agaacttttg tcattctaca
    16261 aagaccaacc agaaatagtg attggcgcag gcacagtatt agacgctgtc acagctcgct
    16321 tggctatctt agctggcgca gagtatattg ttagcccttc ctttgatcaa gaaacggcag
    16381 agatgtgcaa tctgtatcaa attccttact taccaggatg tatgactatt accgagatca
    16441 aaacagcgct gaaaagcggg gtagatatcg tcaaactatt tcctggaagc gcttatggac
    16501 caagtatcat ttctgccttc aaggcgccga tgcctcaagt caatatcatg ccaactggcg
    16561 gtgtgagtct ggacaatatg aaagagtggt tcgatgctgg tgtcgtaaca gtcggagtag
    16621 gtgggaacct tttagcaccc gcagctacag gagattttga caaagtaacg gaagtcgcgc
    16681 aacagtatgc agcaaaaatg aaagaaatca agaggtagct gcttatggga aaagtagtga
    16741 cactcggaga aatcatgttg cgattgtcca cagagcaggg aacccgtatc gattcaagtg
    16801 aacgattcgc tgcccattat ggcggagggg aagcaaacgt agcaatttct ttagctaatt
    16861 acggacatga agcgattttt gctacaaaag tacctgataa cgcattggga aaagcagtca
    16921 aaaagcactt gcaatgttat ggagtagata cgaaacaggt attatctggt ggatcaagac
    16981 taggtactta ttatatggaa actggtagtg gcgaacgcgc cgcctcagtg atctatgatc
    17041 gtgccggatc aagttttgct gaaatggatc atttggaatg gtcgctagag gagttattcc
    17101 aaggaataga tatatttcat gtttcaggaa tcaccccagc tttgtctaaa aaatggcaaa
    17161 aaatgacgaa acaggtgata gaagctgcaa aaaaagctgg ctgcctgatc agcttcgata
    17221 tcaactatcg tggaaaatta tggacacagc aagaagccag cgaagtaatc catcagcttt
    17281 tacctttagt agatatctgc tcagctggaa aaatggatgc tctttatttg ttaggtatcg
    17341 agaaagcgcc agaagcagaa gaacaacctc taatttatta ttatcaaaaa atgcaggaaa
    17401 agttcccgaa tattcaggtg ttttattcga ccaagcgtac cgttcattca gcaagtgaaa
    17461 atgaattgaa ggggacattg tggatgaacc atgaatatgt ggaatcccaa gtccatagaa
    17521 tcaccccaat cgtggatcga gtaggtggcg gagatgcttt tgcaggtggc gtgctccatg
    17581 ggattttatc caatatgtct ccgcaagaaa tcattgattt tgctacggca gcttctgcct
    17641 tgaagcatac gatccatggg gactgcaacc aatttagtca agaagaagtc gcacaatttt
    17701 tagcatgcgg ctctggaaaa atcattcgat agaaggagta gaagaaaatg caagaaatgg
    17761 aaacacgtta tacacacagt ccagaagata tccgtcatta ttcaacggaa caattgagaa
    17821 aagaattttt agtagaaaag gtatttgaac cagggaaaat cagtttgact tatacgcaca
    17881 acgatcgaat gatctttggt ggagtgacac ctactgaaaa agaattagaa atcacattag
    17941 acaaagaact aggtgttgat tacttcttag agcgtcgtga actaggtgtg atcaatatcg
    18001 gtggaccagg attcatcgag atcgatgggc aaaaagaaga aatgaaaaaa caagacggat
    18061 actatatcgg aaaagaaaca cgacatgttg tattttcttc tcaagatgct gaaaacccag
    18121 ctaaattcta tattagttct gttccagctc atcataaata tccaaatgta aaaatcagta
    18181 tcgaccaagt aaaaccaatg gagactggtg aaggcctgac actgaatgaa cgtaaaatct
    18241 accaatacat ccatccaaat gtatgcgaaa gctgtcaatt acaaatgggt tatacgattt
    18301 tagaaccagg aagcgcttgg aatacgatgc cttgtcatac acatgaacgt cgtatggaag
    18361 cttatgtcta ctttgattat gccaatgaag atacacgtgt gttccacatg atgggcaaac
    18421 cagatgaaac aaaacattta gttgtagaca atgaacaagc cattatttcg ccaagctggt
    18481 cgatccattc aggtgtaggt acaagcaatt actcattcat ctgggcaatg tgcggagaaa
    18541 atatcaccta tacagacatg gatatggtgc aaatggatca attaaaataa gcataaggga
    18601 gagaaaatga tggaatttag tatggatatg tttcgtttag atggaaaagt agcattagtc
    18661 accggtgctg tctatggtat tggatttgag attgcacgct ctttagctac agcaggagca
    18721 aaaatcgtat ttaataactt gaatcaagaa tcagttgatg aaggtatagc gcattataaa
    18781 gaagcaggca tcgatgcaaa aggttatgtc tgcgatgtga ccgatgaaga agcggttcaa
    18841 gcgatggttc aacagatcaa agaagatgta ggttcagttg atattctagt caacaatgca
    18901 gggatcatca aacggacacc gatgatcgaa atggatgcgg cagacttccg ccaagtaatc
    18961 gacgtggatt taaatgcccc attcatcgtt tcaaaagcag ttattccgga tatgatcgaa
    19021 aaaggcggcg gtaaaatcat caatatctgc tccatgatga gtgaattagg acgcgaaacg
    19081 gtcagtgctt atgctgcggc aaaaggcgga ttaaaaatgc tgacgaaaaa catcgcttct
    19141 gaatacggtc aatacaatat ccaatgtaat ggaattggac caggttatat tgctacaccg
    19201 caaactgctc cattacgcga aacacaagaa aacggtgaac gtcatccatt cgatcaattt
    19261 atcgttggac gtacgccagc agcacgctgg ggcgagccaa tcgatttggc tggaccatct
    19321 gtcttcttgg cttctagcgc atctgatttc gttaacggcc atgtcttgta tgtggacgga
    19381 ggcatacttg cctatatcgg aaaacaacct taaaagagct ttagtacact gtttagtgga
    19441 gaggttgtga aatcagcgga ttgaacgaat accgaaggac agctgattaa acatcattta
    19501 ttcgaaaaaa gaggctggga cagaagcatt taactccaag aaataagaag gaattcacga
    19561 aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttttacttc
    19621 cgccgtttgt acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc
    19681 ttttctgtgc gtcttccata cttcactaag tcgatccctc ttgcttcgca ccaatccttc
    19741 actggtccgg aaaagatcag agattgttgg tgtagggccg cggtattcgt tgcgccgacc
    19801 atagtataaa gcagacgaag ctcttcttgc cattgcttca tcagtataat agtttcttct
    19861 acgccgtgat tcatcagatg cgtcagtacg gttcctgaag tacccacggc atttgcgccg
    19921 agacaaaggg ctttaaatat gtcataggca ttgcggatac ctccggaggc taggatttcc
    19981 atagaagtgt ctgcttcatt tgcttcaagc aatgaagaga ctgtcgattg cccccagtca
    20041 gctaaataac tcagttcccg cttggagcga cgggcgtttt cgatctgggt gaagcttgtt
    20101 ccgcttcgac cgctgatatc aactgtatga actcctaaag cagctagatc attgagcgtt
    20161 tctcttgtca tgccaaaacc gacttctttt acgatgaggg ggacgtcaat ggctgtttga
    20221 gtttcttgaa taagtacttt ccaattggta aaatcacggt ctccctcagg cataaccaac
    20281 tcttgaggtg cattcaaatg aatctgtaag gcatctgcgt gaaacaatcg gatagcttct
    20341 ttggctcttt cgacagaagt ccctgcaccg atgttggcaa tgattttccc gtgaggatat
    20401 tcttgtcgca taatcgtata agtatcggct aaagaaggga ctttcaaggc agcgctgact
    20461 gaaccagtgg caatcatcaa atctgcttct cttgcgacga ttgcaagatc ttggttgatt
    20521 ttttttgttt tttcgcttcc gccagtcatc gcattgatat aaaatgggct gcttagtgta
    20581 aacccaactg cctgtgtact tagatcgaca tcagaaactg ctatttgcgg caaagggtta
    20641 tggatgatcc gtacgaagtc aaattcgttt ttttgtttat catgaaaggc tttagctagt
    20701 gaaacatgtt cgtcttttcg attcatcggt tttcctttct ttgatcgcta tagacatgta
    20761 acggcagtgg agtgatttct gctttttccc atgcactcat taaaggaaga atgccagatt
    20821 tctggtcaac aattacgatt ccgcaatcgc ccccacctgc accagaagat tttgcggctc
    20881 cttcatactg ttcagctaaa ttacacaatt tgttcaaagc aggcgtttcg atgacgaccc
    20941 cagtgattgc agataaatca tgcagtaatt gtcggttttt tcgaatcatc tgttggatca
    21001 acgttacatt attttcttta aaccctttga tcatttcgtt gacacattct gtactatttt
    21061 ttaagaaaag ctgataagcc accattttat cttctctcga acggtgaact tgatcgacca
    21121 aatcagaagt agaggcaggg ctacccgtcc aaccaatcaa taaacgtaaa tcttcaggag
    21181 caatcaatgg ctcaatggat agacctggcc aatccagggc aagtaactca ctgatagaat
    21241 gctgagtttc ttgttcttgg agccaaggat ggtcgaaggt tgagaaagcg atccagccac
    21301 cataacagct agcagcgatg tcgccgcagg aaccattatc ttgaactgct aaattggcta
    21361 gtgctgcaat cttgaaaatc tccaactgag ataaattcaa cgcgtaaaaa acattcaagg
    21421 ctttgaccgt tgcaacggtc acggcaccgc tagaacccaa tccatatttc cggccatttg
    21481 aactgtctaa ctcacttgtt actttcagat cataaaagga taaaagaatg tttttttctt
    21541 gtgcatactt ttcagtcaag cgaatagcag caaggatata atgaaaagga ttttcccgaa
    21601 tatctaaaac taattctccg ttgcgtcttg tccaacgtac aggcatccca ctatattgtg
    21661 cagattggat acttccgact tttcgtgcag attctacagt gactgtcacg aattgatcga
    21721 ctgcagcgat aactgctgga tggcctgttt caacaactgc atattctccg gcaatataga
    21781 gtttgcctgg tgcagatact tcaatcataa aaatctccct ttattcaata atcgcaattc
    21841 ctggaccagc atgtgcagta attacttgtt ctttgctaaa taatttggta aatgtttctt
    21901 gtacctcaga taaatggctg ttttccacta aaactttgac attagggcca gcatccatcg
    21961 taaagtagca tggaatgcct tgctttctca attgtcgcac cgcatccatc gcttttaagc
    22021 tgtcaggaga ccagtaagtg aatggaggct gggcagctaa agtcgttcca tgcattttta
    22081 gtccgtttct ttccatcgtt tctccgagaa gttggaaatc ttttgttttg attgcttgtt
    22141 tcaattgata tagatcccct tcaacggaat ctaaccagcc ttgataaaaa ctggatgttt
    22201 cgactgtccg acgcatccca tttctgctgg acacttcttt tttctggtcg ttgatcaaaa
    22261 cgaaaaccat tgctaaatcg tcttcgaaag aatcggaagg gactggctta gcgtaagaac
    22321 ttaagtcgtc atggcctttt tcccattcga cgaaaccacc gaaaatactt cggcaagctg
    22381 aaccagaccc gcgtcgagca aatcttgaaa gagagagatc atctaatcca agcttaagag
    22441 cagtattgca agcgcctgct aaagcagcta gcccgctggc agaggaggct aatccagctg
    22501 cggtaggaac gaaattctga ctgattactg aagcttttag tgagaggccg gcttctttac
    22561 gaaaaaggtc aagaaactga ctgacttttt ttgttgcttt ttcgtcttgc aaggtgccat
    22621 ctagataaaa ttcatctacc atatagctat cagaaaagat gacttctgtc tctgtataaa
    22681 atgcatccaa tgttaacgaa aggctgttgt tcattggaag gatgagttct tcatttttct
    22741 taccccaata tttgattaga gcaatgttgg tatatgcgcg tgctttgcct ttaaacatag
    22801 gtatgtactc ctaatccttg tatccaagtt tcagcagcac ccgcatcttc aagtgcttga
    22861 ctgatttctt gggcttttgt ttttgtttgt gctaatgcaa tcatacagcc accgcgtcca
    22921 ccgccagtta atttggcacc caatgccccg gtatcttttg ctgtttggat caatagatta
    22981 agaaaatcat tgctgattgt cagcttttcc agaagtgatt gtgattcatc cattgtctgt
    23041 gccaacgttt ctggtgaatt ttcaatgatt gcttgttttg cttgcttcgt caagtatcct
    23101 aatttttgga tcatttgtcc agtttcatga ggctgggttt caaagaggtg agcgacatct
    23161 ttgacggctt ctcttgtttg tcctttgatt cctgtatcag cgacaatcaa aaaagcatcg
    23221 atgttcaaag aaaagtagtc gaaaggatgc ccttttgtaa aataaatcgg ctgcaagctg
    23281 ctagtagcgg ctgcatcgat tccactagga ttaccgtggg cgattttttc cgaaagctgg
    23341 acattttcta atagtatttc acgagacaaa ggaaatgcta agtaatcata aaaagcacga
    23401 gtgactgctg tggcgactgc agcgcttgat cccattccac gttcagcagg aatcgtgctt
    23461 tcaatggtca gttgcaagtt tttttggatg tcatgctgtt tttttaagtg aatgaataat
    23521 tttttgatat tttttaatgc atgaggagct tcggctaaat ttcctgaata gtaggaagaa
    23581 accagttgat cgtccattgc atccagctct tcaaggaatg cggtgacttt tgttgcatag
    23641 aaaggaaagg caatcgctgg ttctccataa acaaccgcgt gctcgcccat caatatgatc
    23701 tttccgcttg attctccttg gccatagttt gccataatct aactccttct cacgtaaaag
    23761 tgatttttaa gcaaaaaata aagcgcccaa aacgagttgc gctgcttcac gaattttttt
    23821 gtccactaag ggtctcaaaa aacattcctc ctttatttta ctaaaaccat ctcagaagta
    23881 aaactaagaa tacgttttct aaagaaaaaa cacttttttc ttaagaattt tttgactttt
    23941 tttcttcttt gactagaaaa aaatacaatt gacactacta gatattgggg gtatatttaa
    24001 aaaggttgga gttaaacaca atatttagtg atgaagtgag ctggaggtaa atgagcgtga
    24061 tcgttttagc aggtacaatt ggtgccggaa aatcaagttt aacagaaatg atggctgaac
    24121 actttgatag tcaggcattt tatgaatcga ttgatgataa tgaagtcttg cccttatttt
    24181 atgcaaaccc tgaacagtat gcttttttgc tgcaaattta ttttttgaat aaacgttttg
    24241 caagcatcaa acaagcaatg aaagatgaca ataatgtatt ggaccgttcg atttatgaag
    24301 attcactgct tttccatcta aatgcagatc taggaagagc aacagaaaca gaagttcgtg
    24361 tgtatgatga actgctggaa aacatgatgg aagaacttcc ctatgctgcc cataaaaaac
    24421 atccggattt attagttcat attcgtgtat cttttgatac gatgttggag cgtatcgaaa
    24481 aacgtggtcg ttcgtatgaa caattgtcct ttgatccaag tctatacgat tattacaaag
    24541 aattgaacag acgttacgat caatggtatg aagaatacaa agaaagtcca aaaatccaaa
    24601 tcgatggaga ccgctataat tttgtggaag atcctcaagc aaaagaagaa gtcttgaaaa
    24661 tgatcgaaga aaaattagca gagattcgcc aaaccaaagt agccatttga tttacagttg
    24721 ctcaaataaa gggtaaacct ttatttgagc gacttttttc tttatactgg aaacaaatgt
    24781 caggaggata gagtatgcgg gaaaaacact attttaatca agaccgtaat ttttggatca
    24841 atgtacagca aaacgatgtg ctagctgtta aaaacttaaa agaagaatat ggcattagtg
    24901 acgagatgct gacttattct ctagataaaa atgaacgggc gcgcgtagag tacgattctt
    24961 ttgatgaagc gttactgctt gtatcaaatg ttccgcacca gcaaaaagtc gataatcatt
    25021 atgaaactag tcctatcgct tttattttaa aagaagacgg tttgtttaca tttaccacgc
    25081 cgaataccga gtacgtgatt cgactaattc gatcgctttt ggaacgaatg ccagacatga
    25141 gtgtatacag tttattgttt cgtacattgt ttttgatttc tgattccttt tttcctttaa
    25201 tcgaggaagt gaatagtgaa cgtcagcggc ttaatctgaa attaagagaa aaaacaacga
    25261 acaagaatct gctgcaactt tcagatttag agatcggttt ggtttactta gttacaggaa
    25321 caaaacaaaa tgtagtactt ttagaacaaa tcaaggcatt ggctatttat cgcaagttgt
    25381 cagaaaagga aaaagaagag ttggacgatg cgctgatcga agcacgacaa gcagttgaaa
    25441 tgacgaactt agcttcgcag attttagacc agctctctgg cacttataat aatttgctga
    25501 ataacaactt gaatgacacg atgaaatttt tgacggtctg gtcgctgatc ttgaccgttc
    25561 caactattgt gacaggattc ttcggaatga atctccagtt accctttacc cattccgttt
    25621 tcggatgggg aatcgccttg atcatcagtt tggtcttatc tatttggatg cttattgcac
    25681 tgtggcggcg gattcgttga tctagtcttt gatctgcaaa ccaagggctt gggcgatcac
    25741 aagtgcttgc tcttggttga cgattagtcc cgccaaagaa tcttgagata aagcgattgc
    25801 tgtgaagaaa ttagtcgtaa agtctaatcc cttcagagaa gttcgaaacc agttcgaacc
    25861 ggtcaactga ttgtcagaca aaaaaaggtt tttccaagtg acctcataaa attcgctgtc
    25921 ttctaattga caatgatcga aggagacagc tttcaagttt gtactactaa aggaagccat
    25981 cgaagcaata caatcagtga aagtacagtc tcgaagataa ctttcggcaa aattcgtacc
    26041 tgttaattta cattgatgaa acacgacttg gtggaaactg ccaccaatcc actccagatt
    26101 ggaaaaatca catttttcaa aaatgacatt actgcattca aaatgacgta aaactgtttt
    26161 ttgcatcgta agtttttgga tatgactctc tcttaatacc atatttttgg catcgagata
    26221 gctttggtca cccccgttga tcagaagacc agagaagatc tcctcatctt ctaaataaaa
    26281 ctctcccttt gtaagaagag gaagagaagg tggaataggt tcgatttttt tcatgtgggt
    26341 tcacctcgtt tttctttagt atagcaaaga agcgggaatc actgctagaa agtctgctaa
    26401 tctaatcatt ttcagaaaag gaagacaaac tgtatgaaaa acattgacaa aaagattagt
    26461 ttttgctata ctgtgatggt tgagaaataa aagcagaagc acccgcttct cgccttagtt
    26521 gataatgtca ctgggctaga tagttttcac tggttctatt aggtgaagta cgcgcgggtt
    26581 ctgtaagaga actcgttttt ttattgccgt ttattcggta agcttttctt tctctcataa
    26641 tatttggagg tgaatgacca tagcaaagga tatgatggtt aacgacggca ttcgtgcacg
    26701 agagttacga ttgatcggtt cagatggtga gcaattagga gttaagacaa aagcagaagc
    26761 attgaatatt gcagaacaag caaacttgga tcttgtgtta gttgcccctg gtgcgaaacc
    26821 accagttgcg cgaatcatgg actacggtaa ataccgtttc gaacaacaaa agaaagaccg
    26881 cgaagcgcgt aaaaaacaaa aagtgatcaa tgtaaaagaa gttcgcttaa gtcctacgat
    26941 cgacttgaat gacttcaata caaaacttcg taatgcacgt aagttcttgg aaaagggcga
    27001 taaagtgaaa gcttctatcc gtttcaaagg ccgtgccatt acccataaag aaattggtca
    27061 aaaagtcttg aatcgcttag ctgaagaaac tgcggatatt gcaacagtgg aacaaaaagc
    27121 gaaaatggac ggacgcagca tgttcttgac gctggcaccg aaaaacgaca gtaagtaatt
    27181 tgaatgacta actggctgtt ggacagccga tagtcggata acgaaagatt ttgaggagga
    27241 aatatagtca tgccaaaaca aaaaacacac cgcggatcag caaaacgttt caaacgtact
    27301 ggtaaaggcg gattgaaacg cttccgcgcg tttacaagtc accgtttcca cggcaaaaca
    27361 aaaaaacaac gccgtcaatt gcgtaaagca agcatggttt ctagtggcga cttcaaacgt
    27421 attcgccaac aactagcaaa aatgaaataa gaagtacaaa taacagaatc tgaagattta
    27481 tttttattag gaggaattat acatggcacg tgttaaaggt ggaacagtaa ctcgtaaacg
    27541 tcgtaaaaga gtgctaaaat tagcaaaagg ttactacggt tcaaaacaca ctttatttaa
    27601 aacagcaaaa gaacaagtaa tgaattcata caactacgca tatcgcgatc gtcgtcaaaa
    27661 gaaacgcgac ttccgtaaat tgtggattgc tcgtatcaac gcagcagctc gtatgaatgg
    27721 cttgagctac tcaaaattaa tgcacggttt gaaagttgct gaaatcgaca tcaaccgtaa
    27781 aatgttagct gacttggctg ttaacgatgc agcagcattt actgcattgg ctgaccaagc
    27841 aaaagaagct ttggctaaat aagctaaaca attataaaga ggaaagaaga taacgaaaag
    27901 ttatgttctt tcctcttttt ttatttacaa tcctccatca aatgaaacag gacaataacg
    27961 tataagtcta aaatagcata attaaggttt tttaattgaa aactgaacat ttatggtcac
    28021 cctttaatga aagcgctaac tacataagtt agaatgagtt tgactgatat attagtttgc
    28081 ttatcagaaa aacaaaatgg acggaggaat aacatgagtg atattattat tggtattttg
    28141 ttagttatct cattcttttt catggtctgg tactgtgtaa aaggctacaa cttgatggtc
    28201 ggctttgcca tcatggctac tgtatggatg ggattagcgc tagtcggaaa cacgttttca
    28261 ccaaacccgg cgatggaagg gcaaggagta attgatgtgt tgacgcatat ctatacgacg
    28321 gggccagctg agtacgccaa atcgatcttg gtaaatgtct tctttggtgc ctttttcggg
    28381 cgtgttctag tagatagcgg aatcgcagct accctgattc gaaaggtagt tgaacttgga
    28441 ggggacaagc caagaatcac gatgagctta ctatgtattg taaccgctgt tatattcatg
    28501 tcgatgacag gaatcggacc agtgatttct attgcagtta ttgtgttgcc aatcttgatg
    28561 tcattaggta tttcagtacc ggttgcactg ttctcattta tgggctcgat tatggctgga
    28621 atctttgcta atatcgtcaa tttcaaacaa tatcaaacga tttatgctgg tttcaatcca
    28681 gcggctgaaa gctatactta taatgactat ttccaaattg gtatgattgg gatgattgta
    28741 agcttagttg tggtactaac ggtcgctaat atatcaatga acaaaaagaa acgctatgca
    28801 atggcagcaa acgtaccagc tgaaggtggc gacgcaccaa tgatctcatg gttagccgta
    28861 ttattaccag ttttaggggt cgttctttta gatcttccaa tcatcttagg ttttattcta
    28921 gcaggtatct gggcattgct gtttactgga aaattgcgcg gaggttataa agaaatctgt
    28981 cgtcagtttg cgaaattatt tacagatggc gcagtagatg ttgcgccaat ggttggtttc
    29041 ttgatgacct tagcgatgtt caataattca gctgcgtatg cttctcctta tttttcagct
    29101 atttttggag attggatacc gcaatcacca ttagtcttgg caatcgtatt tgctatattg
    29161 acaccactag gatttttccg cggtccaatg aatctagtcg gttcaggctc agctatttta
    29221 gctgtcgtat tagctgttaa tccaacgatg tcgccagcct ttttgttccc gttatttgca
    29281 atcacaacga ttgctcctca gcatttagat attacacaat cttgggttgc ttggggatta
    29341 ggttatacaa aagtaacttc tcgagaatat atgaagaaat ccatcccaac tggttggatc
    29401 atcggagcga tcttatgcct gatcacgttc ttattgtacg ggaacgccta gtaaacaaaa
    29461 cgtcgataaa aacgattaga tgaatggagg aaaagaaaat gaaacgtttt gtgcgaatgg
    29521 gaattgatgt tggcggtaca catacgaaag cagtcgcaat cgataatgcg acgcacgaaa
    29581 tcatcggaaa atcttcagtg aaaacaaccc acgatgatgt ccgtggagta gccgcaggtg
    29641 tcgttcagtc ttttcaaaat tgtttgctgg aaaataatat cagtccagaa gatgttgtgt
    29701 ttgttgcaca ttctaccaca caagcgacta atgcactgat tgaaggagac gtagcaaaag
    29761 tcggcgtaat cgggatggca aaaggtggat tagaaggttt tttagcaaaa cgacaaacta
    29821 gattgaacga tatcgattta gggaataaga aaaaaataga gattgtcaat gcattcttac
    29881 ctgtcaaaca cttaaatgta gaccgtgttt cagaaacgat ttcttcttta gaaagagaaa
    29941 gagcggaggt cttggtttct tctatggcat ttggagtaga taatggggaa ccagaacgag
    30001 ttgtttatga agcagcatcg gtaaaatcga tccctacaac gatggcttct gacatcacaa
    30061 aattatatgg attgacaaga agaactcgga cagcagcaat caatgcatct attttaccaa
    30121 aaatgctaga tacggcaacc tctacagaag attccgtacg agaagcaggg gtcaatgttt
    30181 ctttgatgat tatgcgcgga gacggcggag taatggaaat caatgaaatg aaaaaacgac
    30241 ctgttttaac catgctttcc ggtccagcag catcagtcat gggctctttg atgtacttga
    30301 gagcgtctaa tggtgtttat tttgaagtag gcggaacaac aaccaatatc ggggtcatta
    30361 aaaatggacg tccagcaatc gattattcaa ttgtaggtgg ccacccaaca tatatctctt
    30421 cattagatgt tcgggttcta ggtgtggcag gcggttcgat ggtacgggct aatcagtcag
    30481 gaatcatcga tgtcggtcca cgttctgcac atattgccgg tctagattat gcagtattta
    30541 cggagacaga aaaaatcaaa ggtcctaagg ttgaattttt ctctcctaaa gaaggagacc
    30601 ctgctgatta cgtcaaggta gtgatggaag acggagaaga agtgactatt acaaatacat
    30661 gtgcagccaa tgtccttgga ttagtgcaag aagaacattt ttcttatgga aatgtcccat
    30721 ctgcacgaaa agccatccaa gcattagctg attattgtca tacgacggtt gaagacatcg
    30781 cagaacagat catggaaaaa tcctatgcta agattgaacc ggtgatttta gaattggcag
    30841 acaagtatca cttggagaaa gatcagattt ctcttgtagg tgtagggggc ggcgctgcct
    30901 cattgattac ttactttagt aataaaatgg gtgtgaaata ttcgattcct gaaaacgccg
    30961 aagtgatttc ttctattggt gtagcacttg caatggtccg agatgttgta gaacgtatca
    31021 tcccttcgcc aagtaaagaa gatatacgat cgttgaaaaa tgaagcgatg aataaagcga
    31081 ttgaatcagg cgctacacca gaatcgatcg aagtccacgt agaaatcgat ccacagactt
    31141 caaaagtaac ggctatcgct actggttcta cggaagtgaa agcaaccgat ctaaccaagg
    31201 agatcacaac agaagaagca ctagagcttg ctgcagagga tatgcgatta aataaaaatg
    31261 aagtttgctt attggaaaat acacctttct tttatgtatg cggcgaacag aatcgttcaa
    31321 aaaatgctgg aagtcttcgg atcatcgatc aaaaaggatt tatcaaggtc caacgtggac
    31381 atgcttcatg tatgaaaaca actgcagcca attatatgac agctgtcgaa caactttggg
    31441 aagatatggc agtttatcaa actgaattga ttgcccgacc agaattttat ctctgcttag
    31501 gagcaagagt ctctgatttt acagcgacgg atctggaaca actacagctt ttgatggatc
    31561 tggaagtatc tactatggaa ccggaggaag aagtgatcgt cgttgccgga aatatcaaac
    31621 aaacataagt tagaagagtt actaaacgaa ttacaacaga tccccgaaga aatttgggga
    31681 ttctaccagt ttcggcgaga tttattttgg aaaaagattc ctttgtccaa gcagaaaatt
    31741 cttatacaac aatcaatcga ttgtggtatc gaaacggcat gtagcatcaa aaaaaagtac
    31801 ccttttgccg atgtaggaga aatttgtgaa caaatggctg ttccaatcgt ttcctgtgaa
    31861 tcagagcaaa taaacgaacg aatcactttt gcaacttatg cagaagatga agggattcga
    31921 ttgatgacgg agccgttaga aaaattaaaa tgttcaggac tagcttcgat ctctaaagaa
    31981 acagcgcaag ctttgattat tggacatgaa ctatttcacc acatcgaagc ttctgttaag
    32041 ggaatttata cgcaaagcga aaaaattgtg ctgtggcggt tgccttttta tacacatcaa
    32101 tcaaacattc gtgcattgag cgagatagca gcgatgagtt tttcaaaaga gatgaatcaa
    32161 agcgattttt cgccttatgt attagaggca gtcttacttt ggccatataa tgaaattcgt
    32221 agccaaggga tcttagaaga aataaaaaaa atagaaaaaa gatgtacgga gtatgattta
    32281 gattacaaat aaagagactg tgacaaaagt cgtgtcacag tccttttttt ccgaataaac
    32341 ggtgttctcc tgtcaactcc tcggtcacaa gtcaaaatat tcaacaaaca tgagaagctg
    32401 ttttttgaaa ttgttccttg tgactcggag atgaacgg
//
LOCUS       DAEGIQ010000024        30349 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000024 DAEGIQ010000000
VERSION     DAEGIQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.024"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            181..408
                     /locus_tag="KMT45_000989"
     CDS             181..408
                     /locus_tag="KMT45_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBE7004466.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            424..1590
                     /locus_tag="KMT45_000990"
     CDS             424..1590
                     /locus_tag="KMT45_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBE7004467.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1825..2868
                     /gene="hrcA"
                     /locus_tag="KMT45_000991"
     CDS             1825..2868
                     /gene="hrcA"
                     /locus_tag="KMT45_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBE7004468.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2962..3525
                     /gene="grpE"
                     /locus_tag="KMT45_000992"
     CDS             2962..3525
                     /gene="grpE"
                     /locus_tag="KMT45_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBE7004469.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3576..5405
                     /gene="dnaK"
                     /locus_tag="KMT45_000993"
     CDS             3576..5405
                     /gene="dnaK"
                     /locus_tag="KMT45_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBE7004470.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5556..6722
                     /gene="dnaJ"
                     /locus_tag="KMT45_000994"
     CDS             5556..6722
                     /gene="dnaJ"
                     /locus_tag="KMT45_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBE7004471.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7090..7827)
                     /locus_tag="KMT45_000995"
     CDS             complement(7090..7827)
                     /locus_tag="KMT45_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBE7004472.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7829..9331)
                     /locus_tag="KMT45_000996"
     CDS             complement(7829..9331)
                     /locus_tag="KMT45_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBE7004473.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9590..11584
                     /gene="uvrB"
                     /locus_tag="KMT45_000997"
     CDS             9590..11584
                     /gene="uvrB"
                     /locus_tag="KMT45_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBE7004474.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            11594..14410
                     /gene="uvrA"
                     /locus_tag="KMT45_000998"
     CDS             11594..14410
                     /gene="uvrA"
                     /locus_tag="KMT45_000998"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBE7004475.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14673..15938
                     /locus_tag="KMT45_000999"
     CDS             14673..15938
                     /locus_tag="KMT45_000999"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBE7004476.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15942..16673
                     /locus_tag="KMT45_001000"
     CDS             15942..16673
                     /locus_tag="KMT45_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBE7004477.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            16808..17692
                     /gene="rapZ"
                     /locus_tag="KMT45_001001"
     CDS             16808..17692
                     /gene="rapZ"
                     /locus_tag="KMT45_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBE7004478.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            17689..18687
                     /locus_tag="KMT45_001002"
     CDS             17689..18687
                     /locus_tag="KMT45_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBE7004479.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            18721..19656
                     /gene="whiA"
                     /locus_tag="KMT45_001003"
     CDS             18721..19656
                     /gene="whiA"
                     /locus_tag="KMT45_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBE7004480.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            20224..20880
                     /locus_tag="KMT45_001004"
     CDS             20224..20880
                     /locus_tag="KMT45_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004481.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            21059..21624
                     /locus_tag="KMT45_001005"
                     /pseudo
     CDS             21059..21624
                     /locus_tag="KMT45_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22323..22844)
                     /locus_tag="KMT45_001006"
     CDS             complement(22323..22844)
                     /locus_tag="KMT45_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBE7004482.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            23042..26722
                     /gene="nifJ"
                     /locus_tag="KMT45_001007"
     CDS             23042..26722
                     /gene="nifJ"
                     /locus_tag="KMT45_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBE7004483.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            26911..27798
                     /locus_tag="KMT45_001008"
     CDS             26911..27798
                     /locus_tag="KMT45_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBE7004484.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            27795..28964
                     /locus_tag="KMT45_001009"
     CDS             27795..28964
                     /locus_tag="KMT45_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004485.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            28988..30343
                     /locus_tag="KMT45_001010"
     CDS             28988..30343
                     /locus_tag="KMT45_001010"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBE7004486.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         9958 a         5244 c         6720 g         8427 t
ORIGIN      
        1 agattttttt atgaataaaa taaggacaaa attgtttagg aagaatcatg agtgtaaaaa
       61 aatcatccaa gaagtcgatt cttggatttt ttagtggatt tgttcgccaa atacgtttag
      121 tatgcgtata tgagaggttg taagcaaagc gttgtggtct gagcacttaa ggaggaatct
      181 gtgaaaatag cttttcatat ttttgcagat tttgacttaa tgccgaaggt cagctttgcg
      241 attaccgttt atcgagaggc tgtaaaagca ccgtgtagct ccgatgacaa taagcaagca
      301 tttttagagc taggtttcct tgattctaaa tatgcggctt atttcaatga gttggtgctt
      361 gaacgcgatt ttaagaaggt cgaaaacagg cacgactttt cgacgtaagg agaaagaatt
      421 tcaatgatag aaacaataaa tcccgcagaa aacaaggttt ctgcgtacat tcatatccca
      481 ttttgcgagc atatctgcta ttactgtgac tttaataaag tttttttaga agggcagcca
      541 gtagatgagt atgtggaaat gctgctgaat gaaatgagat taatgctgga aagtcatcct
      601 acagcgaatt tggaaacact ttatgtagga ggcggaacac caacttcttt atctgctaaa
      661 caacttgatc gactattatc aggtgctaga gagatcctgc cttttcaaaa tggtaatgag
      721 tttactgtag aagcgaatcc tggtgatttg acccgtgaaa aacttcaagt gatgaaaaac
      781 tatggcgtca atcgtttatc catgggagtg cagacatttg acaatcgctt attgaaaaaa
      841 attggacgta aacatactgc agaagatgtg tatcaaacaa tgcaattttt agaagcagaa
      901 aattttacca atgtaagcat cgatttgatt tatgcattac cgggacaaac actagaagga
      961 tatcgggata ctttggaaaa agctttggct ttggatctgc ctcactattc tttgtactca
     1021 ttgatcctcg aaaacaaaac aatgttcatg aactgggtac gacaaggaag actcgaactg
     1081 cctgatcagg aaacggaaac cagaatgttt gaagagacga ttgaagcaat ggaaaagcat
     1141 ggacgtcacc aatatgaaat aagtaacttt gggctctccg gacatgaatc tcaacataac
     1201 ctcatgtact ggaacaatga ccactatttt gggtttggag ctggtgctag cggttatctt
     1261 ggagatattc gttaccgcaa caaaggacca atccaacact atttacgccc attacgagca
     1321 aatgaacgcc cggtattgga agaagaaaaa ttgacacgaa aaaatcaaat agaagaagaa
     1381 atgttcttag gactaagaaa aaaaacaggg attactaaaa aacatttcta tgatcgctat
     1441 caaaaaacaa ttgaatcggt gtacggtgat gtgctaaggg aattagagca agaagggtta
     1501 cttgttaatg aagaagaccg tgtttatttg acaccaagag ggactttttt ggggaacaat
     1561 gtgtttgagc gctttttatt tgaaaaatag tttgaagaat atttgttttt tatcgaaact
     1621 tgaaaagaaa aagtttagaa agtttgacaa tgaggtattg aagtattgat ataactggat
     1681 ttacatcaat tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt
     1741 ctcttgacag gagaagggct tcttgctata tttataagtg aattagcact tagaatatat
     1801 gagtgctaat gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg
     1861 atcatccaac actatacgag tagtggcgtg cccgttggtt ccaaaacatt aatgtctgag
     1921 ggagtcaagg ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga
     1981 ttgctgttga agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat
     2041 tacgtggatc atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt
     2101 cagtctctag gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata
     2161 ttatctgagc tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga
     2221 ctgactggct ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca
     2281 gatatgggga atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa
     2341 ctagaaaaaa tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg
     2401 taccataaac ttcgtacgga gattccgatg attcttcata aatatttcca gacgacagaa
     2461 gggatgtctc agttatttga caccgttctt ggtcatgttt ttgaagaaaa ggtttatgtc
     2521 agtggacaaa tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg
     2581 atgttttctt ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc
     2641 aatattcata tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg
     2701 attcaagcaa gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct
     2761 actagtatgc cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca
     2821 gctaaacttg ctgattacta ccgctcgctt gacttgtcaa actcgtaatg tgaataacga
     2881 agctatttgc agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa
     2941 taatgaaagg aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca
     3001 gatgctcagc cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa
     3061 gctgaagcgg aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat
     3121 aaatacttgc gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt
     3181 gagcaattac aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac
     3241 aacctggaac gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa
     3301 ggcgtggaaa tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa
     3361 atccctgcta aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca
     3421 gcgactgaag atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta
     3481 catgatcgcg tgttaagacc aactatggtc attgttgcac aataattgtg cggatataaa
     3541 acttacactt tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact
     3601 taggtacgac taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta
     3661 acccagaagg aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag
     3721 taggggaagt tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac
     3781 gccatatcgg tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag
     3841 aaatctcagc aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa
     3901 aagtcgaaaa agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa
     3961 caaaagatgc tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg
     4021 cagctgctct tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg
     4081 accttggtgg cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg
     4141 tattgtcaac agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg
     4201 actacatggt agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg
     4261 ctttacaacg cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa
     4321 gcacacaaat cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa
     4381 tgacattgac acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag
     4441 taccagtacg tcaagcgtta aaagatgcag gtctgtcaca atcagaaatc gacgaagtta
     4501 ttttagtcgg tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag
     4561 gaaaagaacc aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc
     4621 aaggtggcgt gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt
     4681 cattaggtat cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga
     4741 ttccaacaag taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca
     4801 tccatgtatt acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc
     4861 aattgacaga tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata
     4921 ttgataaaaa cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa
     4981 aaatcacgat caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtgaaag
     5041 atgcagaagc aaacgctgaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg
     5101 atgtagacgc attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg
     5161 acgaagaaga agtgaaaaaa gcagaaacag ctcgtgacga attaaaagcc gctgttgaag
     5221 caaacaacat cgaggaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact
     5281 tgactgttaa actatacgaa caagctgcac aacaacaagc tcaagaaaat ccagaagcag
     5341 ctcaaggcgg agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata
     5401 aataatcagt tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt
     5461 gaaaaatagt ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga
     5521 cgtgaaaatg atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg
     5581 aagtcttagg gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc
     5641 tttcgaaaaa ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag
     5701 aagtatcaga agcatatgag attttaagtg acccgcaaaa acgcgctgca tacgaccaat
     5761 acgggcatgc aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg
     5821 gattcggcgg cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct
     5881 ttggaggagg cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat
     5941 atacggtcga cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata
     6001 atcgggaaga aatctgtcat acttgtggtg gaaacggtgc aaaaccaggc acacaaccaa
     6061 ctacttgtca taaatgtcat ggtgcaggta cgatcaatgt agaacgccaa acgccacttg
     6121 gacgagtgat gagccgtcaa acttgtgatg tttgtcatgg cacagggaaa gaaatcaaag
     6181 aaccttgtcc aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga
     6241 atgtaccagc tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg
     6301 gagaaaacgg cggaccattt ggtgatttgt atgtggtttt tcgtgtagaa gaaagtgata
     6361 tctttgatcg tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag
     6421 ctttggggga tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg
     6481 ctggaacaca gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg
     6541 gtggtgcaac aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg
     6601 aacaacaaag agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac
     6661 agcaagaaga cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt
     6721 agtttttcta gtaaaaatat aaagtagact tgcccaaatt ttctatttct agtaaaaacg
     6781 ctaagtcatt aataattaaa aaccagacga acaatcatac gttcgcctgg tttttttgta
     6841 gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat aagataatca tcatagtttc
     6901 tgaaggtggt atcgactatg tacttgatat cgtcaatgaa ccgatggtac tttcttatgc
     6961 gatggaacag gtagagcgat tgcttgtttg tgcaggagaa caagcgatgc ggctggtaca
     7021 gttaagtgaa aaaacaagaa actaaaaatg cccggtcgtc atgaagcgac cgggcatttt
     7081 tggtttttat taaatattta atactttatt caagaaatct tttgtccgtt cattttgcgg
     7141 attttcaaag atttgttgag gcgttccgtc ttctaaaaag tttccgtcgt caatgaacat
     7201 gacacgattg gccacttctt tagcgaagcc catttcgtgc gtaacgataa ccatcgtcat
     7261 accttgttct gctaactttt tcatgacgtt caagacatca ccgaccattt cggggtcgag
     7321 tgcagatgta ggctcatcaa aaagcatgat gtcagggttc atcgctagag cacgagcaat
     7381 agccacacgc tgcttttgtc caccggaaag catttcagga tacatatctt ttttatcttc
     7441 tagaccaact gtctgcagca atttgattgc ttttttctct gcttcttctc tagattcttt
     7501 cttcaggtcc atcggtgcta aaatgatgtt ttccaaaata gaaagatgag ggaataggtt
     7561 aaagtgctgg aataccattc cgatatgttg tctcaccaag ttgatatctg tgcttttatc
     7621 catcaagtgg gcgccgtcaa taataatttc accgtcagaa ggttcttcta aacgattcaa
     7681 gcaacgcaaa aatgtacttt ttcctgaacc tgaagggcca atgatacaga cgacatctcc
     7741 ttcgttgatg gacacattga tgtcgtttaa tactgtgtta tcgccgtatt ttttgactaa
     7801 atgctcaact aagatttttt ctgccatctt acttcacctt cttttctaaa acttttgcta
     7861 attttgtcaa tgctgtgatc aaaatcaagt acatgatcgc aatgatcaag taaacatacg
     7921 tactttgtgt tgtacgtgca acaatgatct taccagtttg cagcaattcg actacaccga
     7981 ttgcggaaat gatcgttgtg tcttttaatg agataacgaa ctgattgacg aatgatggga
     8041 tcatgatgcg tatcgcttgc ggcaaaatga ttttttgcat tgtccgatta taagaaagac
     8101 ctaaacttct ggaagcttcc atttgtccga caggaacggc gttgatcccg cctcgaacaa
     8161 tctctgcgat ataagcactt gcgttaagag tcaaggtgat aacaccagca aggaagtcag
     8221 gaatcgtaaa tccaacgata ccaggcaacc caaagaaaat aaagaacgca agaaccatca
     8281 ttggaattcc tcggatgata tcgacataaa tactagcaat tgtgcgaaga gcgcgtattg
     8341 gtgctacact gaataatcca aaaatcacac cgattactat agccaaagca aaggagatca
     8401 aagttaggag aattgttctc caaagtcctt tcaacaatgc ttgatagttg ttttggataa
     8461 gtccaagaaa tgttgattca tcagcagtgg aagactcact ggaagtttct gaaccatccg
     8521 ctacatattc tttaataatc ttatcgtatt cgcctgttct ttttaattct ttcaatcctt
     8581 cattgaacat attaagaagc tcaggatttt gtcctttttt aacggcaaat gcatattttc
     8641 cgccagattc ttttttgatc ggtgtttcca attcttgtcc ttgtttgatc gcataaccga
     8701 ttaccggata atcatccatc attgcatcga ttgcgccaac ttttaacccg tcgtacaatt
     8761 gatcagcagc atcaaaatat ttgatagaat agccatattc agattcatgg tcgcttaaaa
     8821 attcagcact ttcggtacca acttttgctc cgactgtttt tcctttcaaa tcttcataag
     8881 acttgatcga agtatctcct tttttgacag ctaattggat cccgctatca aagtaagaat
     8941 cagaaaaatc atagcttttc tttcgttcat ctgttacagt cattccggca atcattccgt
     9001 ctgcttgtcc tgattccaaa gcttgcaaag cagcagagaa accacggtag tccattcgga
     9061 tgttaaatcc ttgcagtttg gcgatgtttt ccatcaattt tacatcgatg ccttcgtact
     9121 cgccctttgc gttttgaaat tcaaatggag caaaagcaga gtcactggca attacgtata
     9181 cgtctttttt gggctctatt ttttgcattg tctcgcttgt gcttgactct gccgttgtag
     9241 tgttgtcagc ttgtgcaaca tgtccaaacg caagtgtcac aagtcctgtc attaagaaaa
     9301 caaatgagtg aaataaatgc tttcttttca taatcaacct cctaaatttc caatatttta
     9361 ttattatagg cgaaaagtgc tgaatcagac aagactgtaa gctcgaaaaa atgaaaaaaa
     9421 tgcgagtttc taacatttta tgttataaaa cgtaacatgc ataataagat aaaaaagtgg
     9481 cgaaagtatg ttcggtgtgg tataattttt tagtaaggaa tgcctttgct ttccttttct
     9541 ttttattggt cgtaaaatga aaaggaatga atgaaaagga ggacgaagaa tgattgagag
     9601 agatacctcc cgacactttg agcttgtctc gaaataccaa ccggctggtg atcagcctga
     9661 agcgattaac caattagtgg atggcgtagt cggcgggaaa aaagcacaaa tcttattagg
     9721 tgcgactgga acagggaaaa catatacgat ttccaatctt attgagaaag tgaataaacc
     9781 aacactaatt attgcccata acaaaacatt ggctggtcag ctgtacgggg aattcaagga
     9841 atttttccct aacaatgctg ttgaatattt tgtttcttat tatgattatt atcagcctga
     9901 agcttatgtg ccttcaagcg atacgtatat cgaaaaagat tcaagtgtaa atgatgagat
     9961 cgacaaatta cgacattcgg ctactagttc attattagaa agaaatgatg tgatcgtcat
    10021 tgcatccgtt tcatgtattt ttggattagg ttcgcctttt gagtaccaaa aacaagtagt
    10081 ttcgattcga cagggagcag aactagatcg caatcagttg attcgtgact tagtcagtat
    10141 ccaattcgaa agaaatgata tagattttca gcgcggtcgt tttcgagtgc gcggtgatgt
    10201 cgtggagatt tttccggctt caagagatga acgtgcctta cgtgtggagt tttttggaga
    10261 tgaggtcgaa cgtatccgag aagtcaatgc actgacaggt gaagttttag gagaaacgga
    10321 acacgtggca atcttcccgg cgactcactt tgtgacgaat gatgaacata tggagcatgc
    10381 cgtagcgaat atcaaagcgg aattagaaca gcgtttaact gtcttgcgaa atgaaaacaa
    10441 acttctggaa gctcaacgtt tagaacagcg aaccaattac gatattgaaa tgatgttaga
    10501 aatgggctac acgtcgggaa ttgaaaatta ttctcggcat atggacggac gtaaagaagg
    10561 tgaaccgcct tatacgctat tggatttctt tcctgaagat tttttgatcg tggcggacga
    10621 atcgcacgta accatgcctc aagtcagagg aatgtataat ggtgaccgag cgagaaaaca
    10681 gatgctagta gattacggtt tccgtttgcc atctgcttta gacaatcggc cacttcggtt
    10741 agaagagttt gaaaaacatg tcaatcagat catttatgtc tctgcgacgc caggaccata
    10801 tgaacatgaa caaacagata ccgttatcca acaaatcatc cgtccgacag ggctgcttga
    10861 cccagtgatc gaagttcgtc caatcatggg acaaatcgat gatctagtag gcgaaataaa
    10921 tgaacgagtc gaaaaagacc agcgggtgtt tgtaacgaca ttgacgaaaa aaatggcaga
    10981 agacctcaca gattatttca aagaattagg gatcaaagtc aaatatttgc atagtgatat
    11041 caaaacgctg gaacgaacag aaatcattcg agatcttcga ttaggtgaat ttgatgtgtt
    11101 agttggaatc aatttgcttc gtgaaggatt agacgtacct gaagtttccc ttgttgctat
    11161 cttggatgcg gataaagaag gctttttacg tagtgaacgt tccttagtcc aaacgattgg
    11221 acgagctgct cggaatgaag aaggtaaagt aatcatgtac gcagacaaag taacagattc
    11281 tatgcgtcta gcgatggatg aaacatcaag acgccgtacg attcagcaga agtacaatga
    11341 agaacatggg atcgttccaa aaacgatcat taaagaaatc cgagatttga tttctattac
    11401 gaaagaatcg gaagatgata caaaagaagc ggtacaagtc tcgtatgaag aaatgacgaa
    11461 agaagaaaaa gatacgttat tgatgaaatt agaaaaagaa atgaaaaatg cggcgaaagc
    11521 attggatttt gaaacagccg ctaatgtgcg agatatgatt ttagaattaa aagcttcaaa
    11581 gtaggaggac aacatggcta acgataaaat tacgattcac ggtgcacgtg cccataactt
    11641 gaaaaatata gatgtaacga ttccacgaga taaattcgtg gtcgttacag gtttgtccgg
    11701 ttctggaaaa agttccctag catttgatac gctgtacgct gaagggcagc ggagatacgt
    11761 agagagcctt tctgcttatg ctcgtcagtt tttaggacaa atggataagc cggacgtaga
    11821 tagcatcgac ggattgagtc cggcgatttc tattgatcag aaaacaacaa gtaaaaatcc
    11881 tcggtcgaca gtaggtactg tgacggaaat caatgactat ttacgcttgt tgtttgcacg
    11941 tgtaggacat ccgatttgtc ccaacgatca tatcgagatt accagtcaat cagtagatca
    12001 aatggcagat caagtaatgg ctttgcctga gcgcacaaag atccaagtgt tggcaccgat
    12061 cgtggtaaag aaaaaaggac aacataaaaa aatattcgaa cgaatccaaa aagagggata
    12121 cgtccgcgtt cgagtcgatg gtgagatcta tgatttaagt gaagtgcctg agctggagaa
    12181 aaacaaaaaa catgatatcg cgatcgtcat cgaccggatc attgtgaaag aagggattcg
    12241 ttctcgtcta tttgattctt tagaggcggc tctgcgattg gcagacggct atgctctggt
    12301 tgacgtgatc ggtgaagaag aaatgttgtt tagcgaacac tatgcctgtc cttattgcgg
    12361 atttactgtt ggcgaactag aaccaagact gttctcattc aatgcgcctt ttggagcttg
    12421 tccagactgt gatggtttag gtgtcaaatt agaagtcgat caagatttag tgattccaga
    12481 tagaacgaag acacttagtc aaggtgctat cgttccatgg aatccaatca gctctcagta
    12541 ttatcctcaa atgttagcgc aggcttgtga aagtttcggt atcgatttag atacaccatt
    12601 cgaagaatta ccggaagagc atcaggatat catcttgaat gggacagatg atcttttcca
    12661 tttccattat gaaaatgatt ttggtggcgt tcgcgatgta gaaacgactt tcgaaggggt
    12721 attgaagaat atcgagcgtc gttatcatga tacgaacagt gatttcacgc gtgagcaaat
    12781 gcgtctttat atgacagaat taacttgtca gacatgtaaa ggttatcgat tgaatcccca
    12841 agctctatct gttcaaatca atggaacgaa tatcgggcaa acaaatgagt tgtctattca
    12901 aaaagcagta gatttcttct caaacttaac gttatctgaa caagagaaag taattgccaa
    12961 accgatttta aaagaagtaa atgacagact ttcattcttg gaaaatgtgg gactagacta
    13021 tctaactttg agtcgcgctt ccggaacatt atctggggga gaagcgcagc gaatcagatt
    13081 ggctacacaa attggatcga atctttctgg agttttgtat attttagatg aaccatcgat
    13141 cggattgcat caacgcgaca acgatcgact gattggctca ttaaagaaaa tgcgtgatct
    13201 tggcaacacc ctcgttgtag tcgaacatga tgaagatacg atgtgggcag cagattatct
    13261 gattgacgtc ggacctgggg ccggagaaca aggcggacaa atcgtggctg caggcactcc
    13321 tgaagaagta gctaatgatg aaaattcttt gacagggaaa tatctttcag ggaaaaaatc
    13381 aatccctgta ccaaaagaac gccgtaaagg taatgggaaa gcaatcaaga tcactggtgc
    13441 ttcagaaaac aatctgaaaa atattagtgt tgaatttcca ttaggagaat ttgtagcagt
    13501 cacaggtgtg tctggttctg gaaagagtac attggtcaat agtatcttaa agaaatcgtt
    13561 agcgcaaaaa ttaaataaga attctgctaa gccaggtaaa ttcaagacaa tttccggcta
    13621 cgaaagtatc gaaaagatca tcgatatcga tcaaagccca atcggccgga cgccgagaag
    13681 taatccagcg acttatacaa gtgtatttga tgatatccgt gggttatttg ctcaaacgaa
    13741 cgaggcaaaa atgcggggtt ataagaaagg gcgttttagt ttcaacgtaa aaggcggtcg
    13801 ttgtgaagct tgtcgcgggg atggaattat taagatcgaa atgcactttt tgcctgatgt
    13861 ctatgttcct tgtgaagtat gtcatggcaa acgatataac tctgaaacat tagaagtgca
    13921 ttacaaagga aaaagcattg ctgatatttt ggaaatgaca gtagaagatg ctgtagaatt
    13981 cttcaagcac attccaaaga ttcatcgcaa actgcaaacg attgttgatg ttggcttagg
    14041 ttatgtgacg atggggcaac cagcaacgac attgtccggt ggtgaggcac aacggatgaa
    14101 acttgccagt gaattgcaca aaatctctaa tggaaagaat ttctatatac tagatgaacc
    14161 aacgacagga cttcatagcg atgacatcgc ccgcttgttg catgtattac aaagattagt
    14221 agatgctggt aacacagttt tagtgattga acacaatcta gatgtaatca aaacagcaga
    14281 ttatatcatt gatttaggac cagaaggtgg agaaggtgga ggaacgatcc ttgcgactgg
    14341 aacaccagaa gaaatcatta acgtaaaaga aagttataca ggtcactatt tgaaaaaaat
    14401 aatggtataa agaggataga tagctgagat agttaaaaaa acataaaaaa atgaattttt
    14461 gcttgtttta ttaatatcag tgttctagaa tcattacatg gtagttcttt ctacactgat
    14521 tggtatagac aaaaacttcc tataagttca atccgttcaa aaaagaattg agcaatcgga
    14581 ggagtttcct ttgattgctt ttttctttat ggtatgatat tccttgtgta gttggaaaag
    14641 aaaaacaaga gattatgagt aagtgagaga taatgatgaa cagtattgaa aatgaagaat
    14701 ttatccctat attattagga agcgacatga acgtttatgg tatggcacgt tcttttaatg
    14761 aagcatacgg caaaatctgc caagcatatg cttcggatca gttagcccct acccgctaca
    14821 gtaaaattgt caatgtagaa gttattccag gatttgataa agaccctgtg tttatcgaga
    14881 cgatgcttcg tttggcaaaa gaaagatatt ctgataagag taaaaaatat cttttgattg
    14941 cttgtggtga tggatatgca gaattgatct cccaacataa acaagagctg tcagaatatt
    15001 ttatctgtcc atatattgat tattctttat ttgaaagact gatcaataaa gtaagctttt
    15061 atgaagtttg tgaagaatat gatcttccgt atcctaaaac attgatcgtc agagaagaaa
    15121 tgttagtaaa tggacatttg gaacaggaat tgccttttga attcccagtt gctttgaaac
    15181 ctgcaaacag tgtggagtat ctttctgtcc aatttgaagg aagaaaaaaa gcttttattt
    15241 tagagactag agaagagttc gatttaattt taggccggat atacgaagcc ggttataaaa
    15301 gtgaaatgat tgtacaagat tttatcccgg gtgatgatag taatatgcgt gtgctaaatg
    15361 cgtatgtaga tgaagatcat caagttcgga tgatgtgtct tggtcatcca ctgttagaag
    15421 atcctacacc agcatcgatt ggtaattacg ttgtgatcat gccagattat aacgaaaaaa
    15481 tctatcaaac cattaaggca tttttggaaa aaatcgaata tacaggcttt gccaatttcg
    15541 atatgaagta tgatcctaga gatggagaat ataaattatt tgaaatcaat ttgagacaag
    15601 gtcgtagcag tttctttgtg acattgaacg gcttgaatct ggctcgtttc gtcacagaag
    15661 atcgagtgtt taacaaaccc tttgttgaaa cgacctatgg aacgaatcaa tcagataaag
    15721 caagattatg gatgggtgtg cccaaaaaaa tctttttaga atatgctcgg gaaaatgaag
    15781 acaaaaaact agctgagcaa atgatcaaag aaaatcgtta cggtactact gtgttttatg
    15841 aaaaagaccg gtcaatcaag cgctggttgt tgatgaaata tatgttccat aactatattc
    15901 caagattcaa aaagtatttt catgtgaaag aaggctagtt catggagaat tttttcagta
    15961 ttttaggcgg aatgggcacg atggcgacag aaagttttgt tcgcttgatc aaccaccgaa
    16021 cgaaagctac aaaagatcaa gaatatttga attatgtctt attcaatcat gcaacagttc
    16081 ctgatcgtac agcttatatt ttagatcgat cagaagaaaa tccaatgccg tttctgctgg
    16141 atgatattga gaaacaaaat ttattgcagc cgaacttcat tgtgttgact tgtaacaccg
    16201 cacactactt ttttgaagaa ttacaagcgg caacggatat tcctatcctt catatgccaa
    16261 gagaagcagc aaatgaactt gtccgtcagc atacgacagg gagagtagcg attctaggaa
    16321 cagaaggaag tatgaaagct ggaatctatg aacgcgaagt caaaaatctt ggattcgaaa
    16381 cagtgattcc tgatactgtt cttcaagaaa aaatcaatta tctgatctat catgagatca
    16441 aggaatcgga ttatttgaat caagagctat attacgaaat cttagaagaa gcggtagagc
    16501 gcctaaactg tgaaaaagtt atcttaggtt gcacagaatt gtcattgatg catgaatttg
    16561 ctgaagataa ccattatcca gtgatcgatg cccaatctat tttagcagat cggacgattg
    16621 aacgagcgct agctgaaaga agcaaggcat tggatacagc atcggaaaag taaaaaaatt
    16681 ggatttgggg caaatatcct aaaagatatt tggtccaaat ctttttgtaa aagaaaaaga
    16741 aaactggtaa tgatacgccc ggaatgatat aattttacta tgcacaatgt gagaggagtt
    16801 cgtaattatg acagataatt tgcaattagt catcataaca ggtatgagcg gtgcagggaa
    16861 aacagtagct atccaaagtt ttgaagatat gggctatttt tgtatcgata atatgccgcc
    16921 tcgattgatt ccaaaattct gggaattaat caaagaatca ggcaaagtca caaaaatggc
    16981 tttaggagtg gatttacgtt ctcgttcctt ctttgaagaa atccaaaaca tgctgatcga
    17041 aatcgaaaat acgaacttca tcgacacttc tatcttattt ttagatgctt cagacgaaga
    17101 actagtggct cgctacaaag aaacaagacg agcacatcca atggcgatgg acggtttgat
    17161 cactgaaggg atacgtaaag aacgggcgat actagacgac ttgaaagccc aagcttccgt
    17221 gatcattgat acgacgacat tgacaccacg acaattacgt gaaaaaatca atgagtcatt
    17281 caaatcaaag ggagaccaag gatttcggat cgaatttgta tcatttggtt ttaaatacgg
    17341 gttgccgatc gattcggata ttgtgatgga tgtgcgtttt ttgcccaacc ctcattatat
    17401 cccggaacta agaccattga ccggaaaaga tcagccagtc tatgattatg tgatgtcctt
    17461 tgatgaaacg caatcattct accataaatt tttggatctt ctggaaactg tattgcctgg
    17521 atacattaaa gaaggaaaaa gcagcttgac agttgcaatc ggctgtacag gcggtcaaca
    17581 ccgttctgtg gcattgactg aacgtttggc aaatgcttta gggaaaaact ataaagtcaa
    17641 tgtgacacat cgggacaagg ataaaaggaa agagacggtg aaccggtcgt gaaaatgaaa
    17701 acctaccgga taagaaaacc aaagatcgta gtggtcggcg gtggcactgg tctgccagtt
    17761 atcctgaaaa gtttgcgaaa tcaaggagca gatatcacag cagtagtgac tgttgcagat
    17821 gatgggggaa gcagcggtgc aatcagagaa tcgatcgcta tggctcctcc tggtgacttg
    17881 agaaatgtct tagtggcgtt atcggatatg ccacaattct atgaagatat tttccaatac
    17941 cgtttcaaaa aggaagatca gtttttagcc aatcatacga tcggtaatct gatcattgcc
    18001 gcagtttccg agatgcgggg aagcacttat gaggcgattc aattattagc aaaaatgatg
    18061 catgtagatg gacatgttta tccttcttca gaaactcctt tgaccttgca tgctgtcttt
    18121 aaagacggaa cgactgcagt aggagaatct aaaatcgcga tcgaccgtaa aaccattgac
    18181 cgtgtgtatg tctgcaacac aaatgataaa agtgaagcca aagcggctcg aaaagtcgtt
    18241 tcagcaatta tggacgccga catggtcgtt ttgggacccg gaagtttatt caccagtatc
    18301 cttccaaatt tagtgatacc agaaatcggc gaagcaatgc tcaagacaac tgctgaaacc
    18361 gtctatatct gtaatatcat gacccaaaaa ggggaaacgg aacatttcac agatgccgat
    18421 catatacgtg ttttacacaa gcatttgaat cagcctttta tcgatacagt attggtcaat
    18481 acggaaaaag tgcctgaaga ttatatggac ccagagatct atgatgagta tctagtacaa
    18541 gtcgcacacg acttcaacgg attgagaaat gaaggatgtc gagtgatttc caccgacttt
    18601 ttggaattga aagatggtgg tgtcttccac gatggagaca aagtagtaga agaattgttc
    18661 cgtttagttt tcggttcaaa atattaagca atttattgtt ggagaaagga ggagaatagg
    18721 atgtcatttg ctgcagatgt gaagaaagaa ctaactggtt tggaagttca tcgagaacat
    18781 gcgaaagcgg aattagctgc tttgctgcga atgaacggct ctttaggtat tgtgaaccag
    18841 cagtttattt tgaacgtcca aacagaaaat gctgcaattg ccagacgtat ctattctctt
    18901 ttgaaagatc attatcaggt acgaagtgag ctactcgttc gtaaaaaaat gaaattaaaa
    18961 aagaacaatg tctatatcgt ccggttgaag caaggcacaa aagatgtgtt gatggatctt
    19021 gatatcatgg atggtatgat gttcaatgga cacgtttcgg acgagattat gggaaattcg
    19081 caaaaaatgc gttcttactt acgtggtgct tttatggcct caggttcagt caataatccg
    19141 gaaacgagcc gttatcattt ggaaatattt tctatttatg aacagcataa ccaagacatt
    19201 tgcgatatgt taaattatta cggtctacat gctcgaacgc ttgaacggcg caatggctat
    19261 atttcttatt taaaaggagc agaaaaaatt gctgatttct taacattgat tggtgcaacc
    19321 aactcaatgc tccgatttga agatgttcga atcgtacggg atatgagaaa ttcggtcaat
    19381 cgtctagtca attgtgagac agcaaatctg aacaagacga ttgatgcggc ttctaaacaa
    19441 atcgaaaaca tcgaattgat cgaagcaaag gtcggtctac atgcccttcc tgaaaaactt
    19501 caggaaatcg cagaacttcg gcttcaacat ccagaggtca gcttgaaaga attaggggaa
    19561 atgattccct caggtgcgat atcaaaatcg ggaattaatc accgaattcg caaaataaat
    19621 gaatttgcgg aaaaactgcg tgaagaagca gtttaatcca aagaaaaaac tgatacatga
    19681 agcaggagat gcgaatgtat cagttttttt taaaagttat acttgctttt cttccgtcaa
    19741 actataattc catctttact ggagtaacat aatttggaaa ggcttctatt gtttttaata
    19801 caatcgtata aaaagcgggg agacaactga atgaagattt tagttttgta cataaatttt
    19861 tgcttaatat agaagtcctg atatagataa gatcaagtat ttatctataa ctggcctgat
    19921 taattaaaaa actggaagga actgctcgat catccttgag aactgctctt cttacttctg
    19981 gtcaagattc tcgtggttgg tggataagga tggttgataa gttcattgga ttagctagtc
    20041 gaaaacaacc aggtgtaatt aatgtagaag ttttaacaag gggatggagt gatttattca
    20101 gtgataatca taagttcata aatagtaatt gagatgtatg gttggaatac gttatacctg
    20161 aacttgaact accaagattt ggtttagaat ttgagaatcg atgtttataa agggagggca
    20221 aaaatgcgtt tttttacaac ttttactatc attatgattg cagtactttt tatttttcta
    20281 gatatagcaa agcgaaatac agcattttta ttatatcggg ttttacttcg agcaggtctc
    20341 ataacattta tatcgatcgt tggatttttt ctattcacag ttattgtatt tatttggaga
    20401 actccagcac caccattgcc agagattaca tatggagaat ttccttttag acttgagtat
    20461 gaattgaatg aagaattaca tgtgatagaa gacacgttaa tagttgaatt tgatggattt
    20521 ggaatgaacg aaggcatagg gagatatcgt agatggacaa gtcgtttagc cagcggagaa
    20581 gatttagtgt tgctgttaga agttagtgat aataagcaaa tattttattt tccaggacct
    20641 gctaattatt acatgggaga taggttgaat ggttataatc atacttttcc tagtgcttct
    20701 ttcattgaaa gggaaagagg cattactaga agggatattt tacatgacaa agaattatta
    20761 gaacaatttg gtccgcttga tcaaaataca ataaatgaag aagaattgtt aaatcaatat
    20821 aatataaggc tagtgaattg ggaaatatct gaaccgattg ttaacaattt tggtgattag
    20881 aaaacaatta cgataaatag aaacaagcca aatcatcagc aagtaaaagc tgatgatttg
    20941 gcctttctta tacgagtagt aggaattcat agcgaatatt attacgatca gtagatattt
    21001 tgatattaaa ggagtattac gaactagtat gtgagaatgt agaggtgatt tgcgaataat
    21061 gagaaattta tcaaatagaa acaaaatatt aatcattatt gttgttatag ctgtgtttca
    21121 tctgggaacg aatgcggttt taagtagaat aatattgggt cctaagccac caagacctgc
    21181 gattacccga ggggagtttg attttagatt agaatatgaa gtagatgggg aaaggattgt
    21241 gatagaagat acaattgttg cattatttga tggattttct gcagatgcag gtagcatggc
    21301 atggtatcgc acgtggcgtt tgcatttggc gagtgacaag agggcacaac aattattgtt
    21361 acatgagtta gatgatggtc gaaaaatata ttatcatttt ggaggagctg gattttatat
    21421 ggggaagtta gtagcagtgt aactgtaaca aggaaagatt tgccaggtct tggcattcga
    21481 aactccatat tttatatcga gcgtgggaga gatacatcta ttgggaacag gaaagggtta
    21541 actcttgaag agttatataa tgactttgga atccgtcttg ttagttggga acacaatcca
    21601 ccaatagaaa atcgctttga ataatcgaaa tctattaatc catttcatca gccagtgaat
    21661 gttgtaatgg aacaatttgg tttagtgttc aaaaccattg aaatactagg ctgtgatcag
    21721 caaaaatgta tctgaataaa aaagagagca actattaaat gagaggagtt aggagttgtt
    21781 agctttatac gaatcaaagt ggcaaataca gatgcgtatg catttgtgtt tgccatcttg
    21841 cgtttggtag acgagatagg tataagctaa agaaacaact tgtttaaaat aaacgaccta
    21901 gctcaacaaa cgaccatcta atcaactaac agcatacata aaagcaacta tttttgaagt
    21961 agaattgaaa tcgggataac ttaaaaagtg attgggatac tagtgtgaat caccgtacat
    22021 aaatattact cgttaaggga atttgagaaa ttttgtatga aaatttaaga attagttagt
    22081 atgcgcaagt caaaaatatg aataaattat ttggtggaga taatttgctc agcttgtcat
    22141 aagaaatgag ataatctggc agatgcaagc ttttggacac catttattcg gaataaggag
    22201 gctgggacag aagcgtttaa ctccaagaaa taagaaggag ttgcttctgc ttccgccgtt
    22261 tataggtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcctacg ctccttattt
    22321 ttttattttg tttggatcaa ttcttttaat tcctctattg aaatagattc atcaaagatt
    22381 tctttaccca agatgacagt cggaacaaat cgaatatgtg cttgctcagc ttcgttgatg
    22441 acagcttggc tcagctgttc gtctttttgt tctgtcagct ttagtttatc tactgcaaaa
    22501 tccgctactt cttggagaga aagatgtttc cactcgtctt gagtatcgaa tatttgtttg
    22561 atctctttga tcgctttttg accatcgctg attgtgagat accggtgcat cacgtttcct
    22621 cgctgcaagc tttctttttc tttatcgaac aatttgatca ctcgctgcaa ttgcccgctt
    22681 tggactgctt cttctaatag ctcataagat tcttcaaacc attgtcggca atacggacaa
    22741 gataggttga tgaattcgac cattttttta ggagcattcg atgaaccata aaggattcct
    22801 ttttgtgtat ttgttttcgt tgcatcaatc actgatatat ccatgttaaa acacctcttt
    22861 cgcttatagt ttatacatgt ctgacaaaaa aaggcaacga tttagactgt ttgtctaaaa
    22921 attagatgac caattcaaat agacatatgt gaaatatttt tataaataaa agaaaaaatg
    22981 gaatgtttta cgttataatg gaacagataa taagagagaa tatgaacaag gatgatgaat
    23041 catgcgaaaa atgaaaacaa tggatggcaa tacggcagcc gcatatattt cttatgcatt
    23101 taccgagatt gctgcaatat atccaataac acccagttcg accatggctg aattagtaga
    23161 tcaatggtca gcagcaggaa aagaaaatgt attcggtcaa ccagtaaaag tagtagaaat
    23221 gcaatccgaa gcaggagcag ctggtgtggt ccatggatca ttgaaaactg gtgcactgac
    23281 aactacatat accgcttctc aagggctgct tttgatgata ccgaatatgt acaagattgc
    23341 tggggaacta ctgccaagtg ttttccatgt tgctagtcgg gcagtgacga caaatgcgtt
    23401 gaatattttc ggagatcacg gagacgtgat ggctgcaaga caaacaggat ttgctatgtt
    23461 agctgaaagc agtgtacaag aagtgatgga tttagcacct gttgcccatc ttgcttctat
    23521 tgaaggagaa gtaccttttt tgaatttctt tgatggattc cgtacgagtc acgagattca
    23581 aaaaatcgaa gttttggaat atgaagaatt agctcctctg atcaatcagg agaaattagc
    23641 ttcatttcgt aaacgaggga tgaatcccaa tcatccgact gttagtggta cgaaccagaa
    23701 tccagacatc cattttcaac aacgggaaac gatcaacccc tattatgata aattacctga
    23761 aatcgttcaa aaatatatga aagagatcaa ccgattacga ggaacttctt atgacttggt
    23821 gacttattac ggagcagacg atgcggaaga agtcattgtt tctatgggat cagttgcaca
    23881 gacgattgaa caaacgattg attacttgaa tgcaaacgga cggaaaacag gttttttgaa
    23941 tatccatctt tatcgtcctt ttccagtgga aacattcgtg caaaagttgc ctcatacagt
    24001 aaaaacaatt gctgttcttg atcgaagtaa agaaccaggg gcaggtggag aacctctctt
    24061 attagacgtt caaagtgcgt tatatgattc tgaacttcgc ccggctgtta ttggtggtcg
    24121 ctacggattg gggtctaaag atgtgactcc cgaccaaatc tcagcagttt ttgatgaatt
    24181 gaaaaaagac ccaagcatac gcaaaaaacg gtttaccata gggatcgtgg atgatgtgac
    24241 ttatcaatct ttggaaccaa aggaaagtct ggatttgaca gaaccgcaaa cattccaggc
    24301 caagttttgg ggatttggat ctgacggaac agttggagca aataaatcag ctatcaagat
    24361 tattggagat cacaccgata aatatgcgca aggctatttt tattatgatt cgaaaaaatc
    24421 tggcggtctg actgtctctc atcttcgttt tggagatacg ccgatacgtt cagcgtatct
    24481 tgtcgagcat gcggaccttg ttgcctgtca cacaccagct tacctgcata gctatgactt
    24541 ggtcaaagga ttgaagccag gtggcatttt tttattgaat actctttgga gcgacgaaca
    24601 attagaaacg catctgccct taaaattaaa acgttacttg gctgaaaata acattagatt
    24661 ctatacgatc aatgccatga gattggccca agaagtcggt ttgggacgcc ggatcaatac
    24721 ggcaatggaa acggcttttt ttaaattagc tgacatcatt ccttttgatg aggtgcttcc
    24781 gctgctaaaa gaagaagccc tgaaaagcta tggacataaa tcaatggcta tcgtagaaaa
    24841 aaacatacaa gcaatcgatc ggacgaccga actgcttcat caagtcgcga tcccagacca
    24901 ttggagaacg ttggagatgc ctgaaaaaat caataaaagg aacactggtt atatacaaga
    24961 aatacttgaa ccagtgaatg cacaggaagg aaatcaactc tctgttggta cacttgtagc
    25021 aaatggaatg acaactggcg aaatgccttt aggaacggct tctatggaaa agcggggcgt
    25081 cgctttagaa gtacctgagt ggattagtga tcgctgtacg atgtgtaatg aatgtgcctt
    25141 tgtttgtcct catgctgcca tccgtccatt tttagcagat gaagaggaaa tggaagaagc
    25201 gccagaagga tttatcgtga gagaaatgcg aggcgcagac ggcgtaaaat accgtatcca
    25261 agtttcagtg gaagactgta caggttgtgg tctgtgtgta gaggcttgtc ccgcaaaaga
    25321 aaaagcttta gtgatgaagc cttatgaaga acagaaagaa caagctgtga attgggcttt
    25381 tgcaatgaca ttaagacaaa aagcaaatcc agtcaaagga aagaatactg tcttgtctac
    25441 acaattcaat caaccgcttc ttgaattctc gggcgcttgc tcaggctgtg gcgaaacacc
    25501 ttatgtaaaa ttattgacac aaatgttcgg tgatcgaatg atgatcgcga atgcgacagg
    25561 ctgttcttct atttggggcg gtgcttctcc tgcttctcct tatacgacca atgaatgtgg
    25621 tcaagggcct gcatggtcaa attctttatt ggaagataat gctgaatttg gttatggtat
    25681 gcttctagcg gcacagactc gtcgcgaggc tttggcaagt tcaatcaaag aagtattgcc
    25741 aaaagtatca gaagaactac gagaattgat ggaagactgg ttggtacaca tgtttgagag
    25801 cgaaggaacg cagcaaagag cagcaaaact gcaagctgcg atcgaaagcg aacgatcaat
    25861 tgtccctgaa ctggaggata tatacaagag aaaggatcta ttcgtaaaaa acagccaatg
    25921 gatgattggt ggagacggat gggcttacga cattggtttt ggcgggatcg atcatgtact
    25981 tgccagcgga gcagacgtga atattcttgt tttggataac gaagtctatt ccaatactgg
    26041 cggtcaaacc tcaaaagcaa caccagcttc ggcaatcgct aaattttcgg ctagtggaaa
    26101 gtatgcttca aagaaagatc taggaatgat ggcgatgaca tatggaaatg tttatgtggc
    26161 gcaaattgct tcaggtgcca atcaaatgca gacgataaag gcttttgaag aagcagaaaa
    26221 gtatcctgga ccttctttaa tcattgccta tacgccttgt atcacacatg gtttgcttgg
    26281 tggaatgaaa gaatcgctta aagaagcaaa agaagctgtt tcttctggct attggtcatt
    26341 atatcgatat aaccctgcaa tggtcgaaaa aaacaagaac ccaatgactt tggactacaa
    26401 aaaacctgat ttctctgcta tgcctgattt catgaggaaa caagttcgct tttcctcgct
    26461 agagaacgca aatccaaatc ttgcaaaagc tttatttgaa aagactatcc aagacgctaa
    26521 aacacgtttt tacaactatg ctgcactgac gggagaatta gaaaaaatca agaaacgact
    26581 tgaaccagag gaatctatcg atgtacccgt taagactaga aaagaaaaaa cgacggacct
    26641 tgaagcagaa gaaagaagag ctgccagagc agaacgacgt aaaaaaatgg aacaagctac
    26701 aaaagaaaaa aaggaaaaat aacagaaaaa atataatgaa taattctctg aagtttaaaa
    26761 atatttattg cagagaaaaa taaaacgcat acttgcaatt ttcctgaaaa ttatgcgttt
    26821 ttctcaagaa aaatacgagc agatcagcct ctaaaattaa aacatatgat atactagaga
    26881 ggaaatctca aaagagaaga gaggcttttt atgtcatttc aaatcgggga actttttaat
    26941 ttggactatt ggcagcaaat gttttccggt gacttgttct ctctgaattt tttggtgaac
    27001 atacttgata ttctggttgt ctggtatctt gtatacaaac tgatccaact gctgcgaggc
    27061 acaaaagcga ttcagcttct gaaaggtgta gctattttta ttgttatccg aatcgtagcg
    27121 gaaatcattg gtctgcacac cttgtcttgg ctgatgaacc aagtcattac gtacggggtg
    27181 atagctgcta tcgtgatttt tcagccggaa atccggagag gactggaaca tcttggacgc
    27241 agttcgcttt tcaaacaatc aaaaagcgaa cagcaggaag atgagaagat gatcttgtct
    27301 tttgacaaag ccatccaata tatgtccaag cgaaaaatcg gtgcgctaat tactatcgaa
    27361 cggcatactg gattagatga gtatattgag acaggaattg cattagatgc agatatcact
    27421 ggagagctgc tgatcaatat ctttattcct aatacaccgc tgcatgacgg tgcagttatt
    27481 gtaaaagaag gaaagatcgc agtagcaagc gcctatcttc ctctatcaga aagtatgttg
    27541 attccaaaag aattcgggac tcgacaccgg gcagctgtcg gtataagcga agtcagcgac
    27601 gcgattacca tcgttgtctc tgaagaaact ggggatgtca gcattacatt agataatgaa
    27661 ctgatggctg gcttatccca gcaagaatat ttggcaattt tgcgtagaga actgattgct
    27721 gaagagaaaa caaaaaacaa gaaaaacgtg attcaatctt tcctagacgg tatgactaag
    27781 ggaggggatc gcaaatgatc tcaaaagaga aacggacaag cattctttac agttttattg
    27841 cattgctgtt cagtattgcg ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc
    27901 tttctggaaa tgaagcgtat aatcaaacag ttactggggt gccaatcaag atttcttatg
    27961 attcaaagcg ctactatatc catggatatg aaaatgcagt gactgtcaaa ctttccagtg
    28021 caaaccgagt tcagttgaat gcagaggcaa acgaagatac acggatgttc cgcgtcacag
    28081 cagatatcac taagctgggc gaaggaacac atgaggttcc gctaaaaatt caaaatttga
    28141 gcagtgctgt cacagcaaag atcgaaccat cgactattac agtaacggtt gagaaaaagg
    28201 taataaaaga tttcgatgtg gaaacgttga ttccttcaac gttcacacca tctggatatg
    28261 aacttgatga tacttccgtt agtcctaaaa aagtctctat cactacaggt gacaaaacat
    28321 tggcagaaat caaacgagta gtagctgatg tggacgcttc gatggtcaca gatgatggga
    28381 ttaatagtga ggtcccaata caggcgctga atgcagcagg ggaagtatta agtatcgtct
    28441 ctgatccagt gcaagtaaca gtgagagctg attcgatcaa gccaagcaag tcggtacgac
    28501 tttatgggat acagcaagga acaccagctg ccggggtaga aagctatgat ttcagttttt
    28561 caaaactgga agcagaagta agcggctcta gcgatttgct tgcatcgatc ggtgacagta
    28621 ttgctgtacc gatcaatgtc tcaggtatca atcatcgaac aactcgtaaa attgaaatac
    28681 cagtagaaga ggggttaagt atcaagcccg aatcagttag tgtggagatt acacccgtgt
    28741 tgcaaacgtc aagcagtagc tctgcttcga cgagcagtcg gacacaagaa aatacaagta
    28801 cgagtagaag tatttcatct gctgattcag gaacggaacc atcgacaaca accatttctt
    28861 ccagttcgga atctgggaca agcagctcga ccacaagtac agaagaaaca tcagaaacaa
    28921 gcgaatcaac acaaatgagc agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt
    28981 tacaactatg ggtaaatatt ttggaacaga cggcgtacgc ggtgaagcca acaaggaact
    29041 tacgccagaa cttgcattta agctgggacg gtgcggaggt tatgtcctaa gccagcatga
    29101 agaagaagga aaacgcccac gcgtgttagt agggcgggat acaagaatct caggtcaaat
    29161 gttagaagaa gcgctgattt caggactttt atcggtgggt atcgaagtgt tccagctagg
    29221 tgtgatctct acaccaggtg ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt
    29281 catgatttct gcttctcata atccagcaca agataatgga atcaaatttt tcggtgcaga
    29341 tggttttaaa ctagtagatg accaagagct agaaattgaa gcattattag atgctgaagt
    29401 ggatgaattg cctcgtccat cagctgaagg attgggaact gtagaagaat ttccagaagg
    29461 cttattgaaa tattctcaat tccttgtaca aacgatcaac ggtgaccttt ccggtttgac
    29521 catttgtgtc gatgcagcaa acggagcaac ttcaacaagt gttaatcgtt tgtttgccga
    29581 tcttgaaaca gatttttata ccatgggcac aaatccagac gggttaaata tcaatgatgg
    29641 cgtaggctct acccatccag aacgtttagc tgagatggtc gttgaaaaag gagcagatgc
    29701 tggactagct ttcgatggcg atggcgatcg tattattgcg gtggatgaac ttggaaacat
    29761 tgtcgacggt gataaaatca tgtttatctg tgctagatac ttggcagaaa aaaatcgatt
    29821 gaaaaaagat acgatcgtta cgactgtcat gagtaactta ggattccaca aagcggtgga
    29881 aggaatcggt ttgaaagatg tcgttacaca agttggtgat cgttacgttg tagaagaaat
    29941 gcgcaaaaac gattataact ttggcggcga gcagtctggg cacatgattt tcttagattt
    30001 taacacaact ggagatggga tgctttcagg aattcagtta ctgaatatca tgaaacagac
    30061 aggtaagaaa ttatcagagc ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa
    30121 tatccgagta agtgataaaa acggtgcgat ggatgttccg gcaatcaaaa aagtgattga
    30181 agaggcagaa gctgaaatgg gatcagaagg aagaatccta gttcgtcctt ccggtacaga
    30241 accattactt cgagtgatgg cagaagcgcc tactgatgag aaagttaatt actatgtcga
    30301 taaaatcgcg gcagtagtca aagaagaaat cggactggat taagagata
//
LOCUS       DAEGIQ010000025        30131 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000025 DAEGIQ010000000
VERSION     DAEGIQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.025"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(42..938)
                     /locus_tag="KMT45_001011"
     CDS             complement(42..938)
                     /locus_tag="KMT45_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBE7004487.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(931..1611)
                     /locus_tag="KMT45_001012"
     CDS             complement(931..1611)
                     /locus_tag="KMT45_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7004488.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1612..2394)
                     /locus_tag="KMT45_001013"
     CDS             complement(1612..2394)
                     /locus_tag="KMT45_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004489.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2387..3304)
                     /locus_tag="KMT45_001014"
     CDS             complement(2387..3304)
                     /locus_tag="KMT45_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7004490.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3468..3791)
                     /locus_tag="KMT45_001015"
     CDS             complement(3468..3791)
                     /locus_tag="KMT45_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004491.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4579..7806
                     /gene="fss3"
                     /locus_tag="KMT45_001016"
     CDS             4579..7806
                     /gene="fss3"
                     /locus_tag="KMT45_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBE7004492.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7919..8233
                     /locus_tag="KMT45_001017"
     CDS             7919..8233
                     /locus_tag="KMT45_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBE7004493.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8246..8620
                     /locus_tag="KMT45_001018"
     CDS             8246..8620
                     /locus_tag="KMT45_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBE7004494.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8630..8965
                     /locus_tag="KMT45_001019"
     CDS             8630..8965
                     /locus_tag="KMT45_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBE7004495.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9048..10388
                     /locus_tag="KMT45_001020"
     CDS             9048..10388
                     /locus_tag="KMT45_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBE7004496.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10466..11140
                     /locus_tag="KMT45_001021"
     CDS             10466..11140
                     /locus_tag="KMT45_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBE7004497.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11133..11297
                     /locus_tag="KMT45_001022"
     CDS             11133..11297
                     /locus_tag="KMT45_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004498.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11373..12515
                     /gene="dcm"
                     /locus_tag="KMT45_001023"
                     /pseudo
     CDS             11373..12515
                     /gene="dcm"
                     /locus_tag="KMT45_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12505..12795
                     /locus_tag="KMT45_001024"
     CDS             12505..12795
                     /locus_tag="KMT45_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004499.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13054..14238
                     /locus_tag="KMT45_001025"
     CDS             13054..14238
                     /locus_tag="KMT45_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBE7004500.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14235..14372
                     /locus_tag="KMT45_001026"
     CDS             14235..14372
                     /locus_tag="KMT45_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBE7004501.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            15118..17028
                     /gene="ltrA"
                     /locus_tag="KMT45_001027"
     CDS             15118..17028
                     /gene="ltrA"
                     /locus_tag="KMT45_001027"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBE7004502.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            17132..17356
                     /locus_tag="KMT45_001028"
     CDS             17132..17356
                     /locus_tag="KMT45_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004503.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            17473..17970
                     /locus_tag="KMT45_001029"
     CDS             17473..17970
                     /locus_tag="KMT45_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBE7004504.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            18057..18449
                     /locus_tag="KMT45_001030"
     CDS             18057..18449
                     /locus_tag="KMT45_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBE7004505.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            18433..20880
                     /locus_tag="KMT45_001031"
     CDS             18433..20880
                     /locus_tag="KMT45_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE7004506.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            20883..23066
                     /locus_tag="KMT45_001032"
     CDS             20883..23066
                     /locus_tag="KMT45_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBE7004507.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            23063..24064
                     /locus_tag="KMT45_001033"
     CDS             23063..24064
                     /locus_tag="KMT45_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBE7004508.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            24079..24993
                     /locus_tag="KMT45_001034"
     CDS             24079..24993
                     /locus_tag="KMT45_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBE7004509.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            25238..25354
                     /locus_tag="KMT45_001035"
     CDS             25238..25354
                     /locus_tag="KMT45_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBE7004510.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            25370..27289
                     /gene="tet(M)"
                     /locus_tag="KMT45_001036"
     CDS             25370..27289
                     /gene="tet(M)"
                     /locus_tag="KMT45_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBE7004511.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            27387..28760
                     /gene="tet(L)"
                     /locus_tag="KMT45_001037"
     CDS             27387..28760
                     /gene="tet(L)"
                     /locus_tag="KMT45_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBE7004512.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            29324..>30131
                     /locus_tag="KMT45_001038"
     CDS             29324..>30131
                     /locus_tag="KMT45_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBE7004513.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERD"
BASE COUNT         9805 a         5348 c         6316 g         8662 t
ORIGIN      
        1 gataatacat ttttctacta gttctcacta atcttgtata attaaaccct ccgcaacttg
       61 acggagatgg agaagatgcc cccgtctaat ctagcggtca tctctcctcc cattccttcc
      121 accaattttt ttgacaggta aagcccgatt ccgctagatt ccgtatttgt gcggctggta
      181 ttttcggtat aaaaacgctg gaagactttt tcaacagagg tttgcgttcc tggtttgata
      241 ggattggcca ctgttaatcg aatgaatgga tcctcttttg aaaatgtcac ctttgcttgt
      301 ccatctgcat aacgcaataa attctgtatg atattttgca gcactcgcag caacatgagc
      361 ggatctgcta atacagttgt atttatcgag tgttcagcga acgctacctc gatatttttc
      421 tcagaaaatt gttcataaaa agagaaaaat acttcttcca cctctttaga aaaagaaagc
      481 gtatggagat ccatttcttt ttgcttcgtc tctattcgag caagatcata aaactgatcc
      541 gtcatactga gtaatcttgc aacagattgt ttgatgatcg ttaaatactt ctcccgtttc
      601 tccgtatcct ctacctgttc cagtaattgt acataaccgt taatagcagt caaaggagtt
      661 ctgagatcat gagaaaggcc gacgatcgat agtctgatgt tttcttccat ttcttgaaat
      721 gcttgtcctt tttgttcatt ttcatccaac catgtattca gtgcatctac taggtccatt
      781 agattctttt ctcgaaattc gactgaaaga cgaccaccat attcttgtcg ttcagaaaga
      841 gtggcgattt tcttagttaa tctcttgatt tccctttgca ggaagaaaat aaaacttccc
      901 ataaagataa caacgagaag caacagccat ttaatcaaat ctaaacccca ctccccacac
      961 cgtatgaata tacacagcat gtgcctttgc tagcttccct cgaagattgg aaatatgcat
     1021 attcactgtt ttctcactat caaaatatgg ttcttgccaa actgtttcat aaagatttgc
     1081 tcgcgaaaat acttttttcg gatgttctaa aaaaagcttc agcagatcga attctcttcc
     1141 tgtcaaatgg acagccacac cattcacatt tacatccctc gactctttgt ccagtaagat
     1201 ttccttgtaa tagatgcgtt gcgacagatc ttttacttgg acagaagtct gatgacgaag
     1261 ttggatgccg attcgtaaca aaagttcttc gatatcaaat ggtttcgtca catagtcatc
     1321 cgctcctgct gccaacaagg aaactttatc tctttgatcg ctttttgctg aaagaacgat
     1381 gatcggaaca ttcgatgtct gtcggatttc tgctaacaat gcttcacctg aaagccctgg
     1441 aagcatgaga tctagaagga tcaaatcgac aggatatgtg cttaacaata attttccttc
     1501 cgtacctgaa taagcatcaa gaagtttgta tctctgttca agaacctcct ccagtaagga
     1561 atgaacagct tcatcgtctt cgataatcaa aatagtttcc agcaggacca cctacttaat
     1621 gtcagatttt tggaaaataa acattcctag agctgatgat accacgattg ttactagact
     1681 gaccagaatc attttcggtg cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat
     1741 gagccacgtt tccgtaaacc actccgttag aaattccaag cctttgattt ttgcgaaaac
     1801 accgggaatg aaggccgttc ccatgatata aagaaaagaa agaagcaaac tgacactggt
     1861 acttctagtg atgaatgtca aagccactgg aacagataat gtccctaatg tcagcagaag
     1921 caatacagga aatactttta gaacataatt tccatagttt ttgattccct caacttcacc
     1981 aaatccgaaa gcaagtgtgt aagcaagcat ggctagtccc gtgatcaaaa ccacgccaag
     2041 tgttgtgatc aaaagcgtaa cgagaagttt cgacacataa taagtcattc gattccgtcc
     2101 taaagataac gtattgcgaa tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc
     2161 aaatgctgcg aaagccagaa gaatccatgc agccattttt tctaccatta aaatccctgt
     2221 tgcaccgttg atgactgtat ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat
     2281 ataccattcg ttccaagaat aaaaaagcgg aagtacgacc atcagtccta gtgagaccca
     2341 cattttacgg ctcttccata cttttatttt ttcagcagtt aatagattaa acatgtggat
     2401 tgcctccagt taattgtaag aaataatctt ccagtttttg cccaactagg tgaataccat
     2461 ccacaggaac ttttgcttct accaatgctt gattgatggc tgctacttgg tcaattcctt
     2521 caaaaagctg gataacacgg ctagattgta caaaataatc cctataacca agagagtcga
     2581 gaaccgttac tgcttgttcc gtcgccgaag tgtcaatttt tataaattga cgagattctt
     2641 gtgccaattc ttctttcgat aattctttga tcaagtgtcc ttcatgcaaa ataccatagt
     2701 gagtggcgat ctgtgacaat tcatctagca agtggctaga aagcaagacg gtgatgcccc
     2761 gttcttttac caaacgttgg atgatttctc gcatttctgc catccctgct ggatccatcc
     2821 cattcgtcgg ttcatccaaa atcaaaaatt ctggattcgt gatcagtgca tttgcaatag
     2881 ctaaacgctg gcgcatacct aaagagaaat tctttgcttt ctttttccct gtattttcca
     2941 atcgcatcat tttcagcagg tcttcgattt ctctatccga cacaccgccg ttcgctgctt
     3001 ggatacgtag gttatctctt gcagttaatt ctggataaag agcaggggtt tcaatcgatt
     3061 gacccatacg tcgtcttgct gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt
     3121 catcaatcga gatcaaaccc ataatagttc tcataaaggt agacttaccc gctccatttt
     3181 ccccaatcaa tccatagatc attccacgtt tgatttcgat tgatacttta tctaatgctt
     3241 tggcagcacc gtattttttg gttataccag ttgctttcaa gaccgtttca tttccaaaat
     3301 ccatacttat ccttctttct tattgatgag tgaagtatag ttgacgggta taaagaaagc
     3361 agccagaaat gtataaagaa agtgtaaatt ttccaattga atgtcaaaaa aaacaatcgg
     3421 acaatacgtg tctttcctat tatcaggcag tttgttatac gcttgtatca gtttcctgat
     3481 tctttctttt cgaccaaagt cgtttctctg tattcctctt cactgaaaat ttttgcttct
     3541 ttcagatcaa gcttgagtaa ttttaccaga ccggttataa ggataactat agcaagactc
     3601 cctcccagaa aaattagact gtctcgcaga cgtggttgcg ttgtcttcca atcaaagagc
     3661 gcaccaaaca gtgctaatcc aagtggactg gccaccatgg taatcgttgt tatcgttgac
     3721 ataagtcgtc cttgtatatg cgttggaact tctcgctgaa gccagaccat ataaaaagca
     3781 aaagaaaaca ttaatcctta aaaacggact tatgaaaaat aatacatgcc gtttgggatt
     3841 tacaattagc taccaaataa gaggttatga caaaagactt ttcacaatcc cttcttccga
     3901 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatttat caaacatgag
     3961 aagcaatttt tgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctca
     4021 gaagcggata aataatattg tttgatgata ctttatcttt ttcccgtttg tttatagatc
     4081 gaatgatatt atagaccaag aatcctacct attaacatag ctaaaaacac aaaaaaggca
     4141 ctttccaaat tgtggaaagt gccagaaaag ttaggattaa ggtaactgcg ctcagcattg
     4201 cttcgcccag tacgattctc gtaactcgtg aactcgaacg ataatcgcaa ttaacgtttt
     4261 gagaactgtg aagctttacg ggctttctta agacctggtt ttttcttcgt atcattgaat
     4321 aagtttgaaa aaccttgatt tataaaggtt ttcccgtaaa tatattttat tatatttgat
     4381 taaatttgaa atgtttgtgt tctaaaatgt gttctaattt aaaggcagta tatttcgtat
     4441 tttagattaa atttttaggc tcattcattc tcaaatccat tctattcagt caataaaaat
     4501 tttaaaaaga ctttataaac aaagatgttc aagcgagcat cttatttttt tgtgcttaat
     4561 ttgaaaggag aacatttcat gtataaaaaa caaaaaaaga aattatgtgt attaactatt
     4621 ttgagtttgg ctgttcaagc aattgttgga gcaattgctc ctacgattac ttttgctgat
     4681 gaaattaccc atccacaaac ggtaaccgta gaactagatt tagcacatca atatgctgtt
     4741 gaaggaactt ttagtgatgg tcgtccaatg tcagaagtca ctgttccgca ttatgcagtg
     4801 tacaacggct ttaaacaaga tattttctgt attgaaccag gagtcccgat ttacaatgag
     4861 ttcactcctg gctatgagaa aaacccgtta ccagatatgt ccgataaagc caaattagtt
     4921 tccgttttat ggaaaaaagt aggtacagat gcagatactc atattgtggc acagaagatg
     4981 atctggcaag aagttaatgg gtataccctt cactcaataa aacgatcaga tggtagtgcc
     5041 gtaaatatcg cagccattga ggcaaaaata aatcaagcca ttgacgatta tcagaagaaa
     5101 ccaaccttcc ataatagtac ggctaaaaca gtattagggc aatcgactac tgtgacggat
     5161 acgaataatc tgaatttgtc agagttcgat gaggttgtgg aaaatgccgc aaacattgat
     5221 tatcgtgtga atggtaatca gttgataatc accccaaatg ccaattcaaa agaaagtggt
     5281 gtgctgacgc ttaagaaatc tgctggtacc ggaacacctg tcgcctataa aaaggctggc
     5341 caacagactt taatggccgg ggcgattgat aaacctaata cctacacaat caaaattgat
     5401 gtggaaacgg agggttcttt aaagatcaaa aaagtcgata aagaatcggg tgatgttgta
     5461 ccaggaacgg ttttccattt agactttgga aaaactttac ctgcaaaaga cgtgactact
     5521 gacaaagaag gcattgctac attggatgag attcctcatg gtacaaaagt aaccattacc
     5581 gaaaaatcag taccggcgcc ttatacgatt gataccaccc ccatgactac caccattaaa
     5641 gcaggcgaaa ccatttacgt gacttcaaaa aacgcacggg aaaaagggca aatcattctg
     5701 gataaaagcg gagttgaaac agggagtgat ctttggaatg acaattattc cttagctggt
     5761 aatacgtttg ccattcgcaa agacagtcca accggtgaaa tcgttcaaga aatgaccacg
     5821 gatgaaaacg gtcatgcgga aacaccaaaa gagattgcta atgcgttgga attgggaacc
     5881 tattatgtga ccgaaactaa agctagtcat gggttcgtga atacgttcaa acctgtaaaa
     5941 gtcgaattga agtatgccaa tcaaaccgta gcccctgtta ccagtaacgt aaaagggcaa
     6001 aatcaagaag tcactggcga aaccactttg acgaaagaag acaaagatac tggggataaa
     6061 gcacaaggta aggcagtctt tgagggtgct gaatacacct tgtttactgc taaggatgga
     6121 aaagctgtta aatggagtga ggcttttaaa ccagagttag cgaagggaac gaaaggttct
     6181 gatgaaacgg tcactttgac tttagacgaa aagaaccaag ttgccgttaa acatttagct
     6241 atcaacgagt attattgcca agaaacaaaa gcgcccgaag gatatagcct tgatgaaaca
     6301 aagtatcctg tatccatcaa aaaggtggat gatagcgaaa aaaatgccgt gattacccga
     6361 aatgttacgg caaaagaaca agtgattcgc tttggctttg atttctttaa atttgctgga
     6421 tcagctgcgg gcactaccga aactggcttc aatgatctga cctttaaagt gtcgccattg
     6481 gaagggacca atgaaatcac aggtgccgaa gatgaagcga ccacagctta taacgagcaa
     6541 ttagggtttg atggttatgg caagtttgaa aatcttcctt atggggatta tttacttgaa
     6601 gaagtagaag ccccagaagg ttttcaaaag attacgccat tagaaatccg ttctactttt
     6661 aaggaaaaca aagaagactt tgtgaagagt gagtatgtct ttaccattac cgaacaagac
     6721 caaaaacaac caattaaaac ggtgaccgtt ccttacgaga agctgacgaa caaagcattt
     6781 tctgttagtt tgaaccgttt aatgctctat gatttgcctg aggaagaaga tagtttaact
     6841 tctcttgcga cttggaaaga cggaaacaaa gaattaactt ctctggattc taccgagcta
     6901 gttgataaat tgagttacaa tctacatgaa atcaaagaag actggtatgt ggtagcgcaa
     6961 gccattgatg tggatgcgac aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg
     7021 gtgattgcag aaacaagcgc aacgttggcg aacaaagaga aaacaggaac ttggaaaatt
     7081 cagcataaat taaccgccga acaagtttta aataaaacta ttgtcttatt caactatgtg
     7141 tatgaaaata aggaagcttt tgaagcaggc gataagccag tagcgaagga tgttagcttg
     7201 aataaccaag cccaaactgt cagttgtaca gtggaacacc atgtttctat ccaaacaaag
     7261 gcccacctag aaaacggttc tcagaccttt actcatggtg atgtggtgga tatgtttgat
     7321 gatgtgtcga ttactcatga tgtactggat ggctcaaaag aagctttcga aacaattctg
     7381 tatgctttat taccggatgg tacaaacaaa gaaatttgga aatctggcaa gattgattat
     7441 gaagtaaatg ataaagaatt caccaaaact gtgcttgcga aaaaagtaga taccggaaaa
     7501 tatccagaag gaaccaagtt tacttttgcg gaaatcaatt acgataaaga tgggaatatc
     7561 aatggaaaac acaatgaaga tttgaaagaa aaatctcaaa ccttaacacc aaaagaagtt
     7621 ccaaccatac taagtacgcc gaaacaaccg gaaacaccaa ctgttccaag tggctctcaa
     7681 gaatctagtc ccacagtgaa gaccttccct caaaccgggg agaaaaattc caatgtttta
     7741 ctgttcattg gttttacttt gatttttgcg acggcgggct attatttctg gaatcgccgg
     7801 aactaaggtg atgagatgat acaagtaaat cctaagaacc aacgtgcgcg accgcctcct
     7861 aaaaaagtag tcgcgctatt tttgtatcca aaactaacaa tgaaaagagg aaaagaagat
     7921 ggaattgaaa tttgtcgtgc cagatatggc tgaaaccttt ggaaagttaa gttatgccgg
     7981 tgaaggagaa attataacag aaggatatgg acggaatact acggtgattg gtcgcagtta
     8041 tcatttgtat tccagtaagc aacgggccga tgatattgag gtggtagtag ctgcggaagc
     8101 cggtgaaaag gattttgatc aagaccaacc gctaaaagcc gtgaatcccc atttggttgc
     8161 caaaggctat gagattgaga atcgtgggtt tactgattac gtgttgtatg ttgatgattt
     8221 agtgaaagca taggaggaaa agaagatgag attaacagaa ggtattgtcg tagattcagg
     8281 tttaacattt gggaagttgc gtttttccgc attacgccga gaagtacgga agcaaaatga
     8341 ggatggaaca attggtaatg aggtaaaaga acgaacctat aatttaaaat cctctgcaca
     8401 agggcgaatg attcaagtta gtatcccagc taatgtaccc ttacgcgagt ttgcttatga
     8461 tgcagaggta gagttggtca atccaatcgt ggataccgtt gccaattatg tttttcgaga
     8521 aggaactacc gttaattggt tcattaaggc ggatgattta gtcttgaagc ggcagccgaa
     8581 tcaaggaaat cctacgaacc aaaatgaaag aaagaaatag gtgaagatta tggaaattaa
     8641 tgttgaacat attttagatt gtctcgatca atatggaaaa ggcgagctga ctgaggaaca
     8701 actcgttaaa gcattaacct ctgatgaaaa aatgtttctg attatacatc aaggtttact
     8761 tggagaaaat agcgacagtg aagaagactc tgatgtacta gactggttag gagaagaaga
     8821 gtctttcttt atagttgtcg aagtaaatga aaacttatgt cggcaggcag aatccatttt
     8881 ggaagaaatt ggtgtggaga tgccggatgc tattgaaggc tttttaaatc aattggttga
     8941 aacgaaacaa ctcccctcct cgtagcagta aatgactagc ataacttttt gaaattgcct
     9001 aatgaaatcc tattgggctt tttcgattgg aggtggaaac catcaaaatg tacaaaggac
     9061 atcgcatacg cgcaggagat cagcacttgg tttatcactt tgttcttggc tggctactcg
     9121 ctctgttcat cggttggatg agcgtctttt attttcaaga gtttagacaa tttgatattt
     9181 ctaagctatc actttctact attgaaatcg tttggtctat aaaagattta gtatgtctgc
     9241 ttggaagtct cgctttttca ggagctatga tattgcttta tattcacttt tttcttgatc
     9301 attggcgaag cttgtggcat cgacaaaagc tagctcggat gattctagag aaccattggt
     9361 atgaagtgaa acaaactcaa agtgaaggct ttttcaaaga tttgaatagt agccgaacca
     9421 gagagactat cagttacttc cccaaaatct gttatcgaat gaaagatgga ttactgtcta
     9481 ttcgcgttca aatttcactt gggaaatacc aagatcagct tttaaaattg gaaaagaagt
     9541 tagaaagcgg actgtattgt gagttagtag aaaaggaact caaagactcc tatgtggagt
     9601 atactttact gtatgatatg atcgccaatc gaattgggat agacgaagta gtggcagaaa
     9661 atgggactct gcgattgatg aaaaatcaag tatgggctta tgattcttta ccccatatgt
     9721 taatcgctgg tggcacgggt ggagggaaga cctatttcct cctcaccatt atcgaagcac
     9781 tattgaagtc agatgcagaa ttgtttatcc tcgatcccaa aaatgcagat ttagctgatt
     9841 taggtacggt gatgcctcat gtttattcac aaaaggaaga aatttctgct tgtgtggaag
     9901 atttctatga acgtatgatg gctcgtagca aggcaatgaa agaaatgtct aactacaaaa
     9961 caggagagaa ttatgcctat cttggacttc cacccaactt tttaatcttt gatgaatacg
    10021 tggcttatat ggagatgtta acgacgaaag aaagtgcggt gattttgaat aagctcaaac
    10081 aaatcgtgat gttgggtcgt caatctggtt tctttctgat tttggcttgc caaaggccgg
    10141 atgcgaaata tttaggagac ggaatccgcg atcaattcaa ctttcgcgtg gctttgggtc
    10201 gtatgagtga actcggatat tccatgatgt ttggggaggt cgaaaaaaac ttctttatga
    10261 agcacatcaa aggtcgaggt tatgtggata caggtggcag tgtgattagt gagttttata
    10321 cgccacttgt accaaaaggg tatgatttct tagattcaat tagtaaatta gaaaagatga
    10381 atgattgagt gatcttcaat actgacttta tacttcaagt aacgtataat acttttatat
    10441 agtaggaaga aaggggataa ttaatatgga aagtaatgat tcttatttag agtatgcaaa
    10501 agcaaataag gataagttta tttcttatat tatcaaaggg aaatcccccg aagttggacg
    10561 agacgcagtt tttatggcag ggagtcctgg ggctggaaaa tcagaggttg ccattggatt
    10621 agctgagaat tatgataacc atgtgattat tgatgcggat tactttcgca cccagtttcc
    10681 tgattataat ggaaaaaact ctagtctctt tcaaaaagca tcttcttggt tggtagagca
    10741 ggcatttaga tatgtcttag atcatggcta ttcttttatt ctggatgcaa cttttgcaat
    10801 attaagtgct gaaaaaaata tctcgcgggc gttgaaaaaa cattttcgag tgacgatttt
    10861 ctatgtttat caagacccta aaatcgcttg gaaatttaca caagaacgtg aaattgcaga
    10921 gggacgtcat gtgccgaaaa aggcatttat taacgctttt ttcaaatcaa gagagaacat
    10981 tgcaaaagta aagagtaaac atccagatgt attgcttcat atcataataa aagattatac
    11041 gaataatatt tccgaagttc attatgctac ggataatcta gaattatttt taccactagg
    11101 gtatagtagg aaagatttgg aggttcaatt acatgactga gaaagaacaa gtaacgaaaa
    11161 ttgtaaagaa atacaataag agcattgcgg atttatcgga aaatgctact gcaaaagagt
    11221 tcaaaacggt gattaaatat gtagccgatc aggccaatga aaaacaacgg aaattagttg
    11281 gattaaataa aaaatagtag gacaaattaa taatgtatga gccaagagca aaatgttctt
    11341 ggcttttttc tttgcaatga atgaggtaat gtatgagatt tttagattta ttcgccggta
    11401 ttggtggctt tcgtttaggt atggaacagg cgagccatca ttgtatcggt ttttgtgaaa
    11461 ttgataaatt tgctagacgt agttataaag caattcatga tacaagtaag gaggtggaaa
    11521 tgcatgacat cacaagtgta tcagatgagt ttattcaatc cctcggacca gtggacatcc
    11581 tttgcggcgg atttccgtgc caagcttttt caattgcggg aaagcggcaa agattttccg
    11641 atactcgagg tactttattc tttgaaatcg ctcggttcac cactcttctc cgacctaagc
    11701 ttttattcct tgagaacgtc cgaggattgc tcaatcacga aggaggggct acgttcgaga
    11761 cgatcctccg aacgctggat ggattggggt atgacgtgga gtggtaggtg cttaactcaa
    11821 aggcctacat tccccaaagc cgggagcgga tattccttat cggacattct cgagacgcat
    11881 gtaccgaaca agtatttcct atcattggac cgtcttcaac atctgatcaa aacatcagaa
    11941 atttggtaaa tatcaatcct tccaatcgag gaatgggtgg acaagtctat ggttcagatg
    12001 gcgtagcgcc aaccttaaca ggtgatgaag gaatcaaaat cgcactgcca gcaagggatg
    12061 gaatctctgt agcaggacta ttaccgggaa agtttgaaca aggtaatcga gtttatgaag
    12121 ttactggaac cgcccccacc ttgtcaacga aacaaggtgg tacaaaaatc atgattcgaa
    12181 aaaatgaaac agaatatcaa gaggtgaagc cagaagacag tgtgaatctg gcttttccta
    12241 attccacaag tcgccgaggt cgtctgggaa aacagagtgt tcatacctta ctaacaggag
    12301 accaacaagc gatcgtcacg gatcagtatc aaattcggaa gctaacacct agagagtgtt
    12361 ggcgacttca gggctttccc gattgggctt ttgatcgaga ttctcaagtc aattcagata
    12421 gtcagttgta taagcaagcc ggaaactctg taacagttcc ggtcattttt gatattgcca
    12481 gaagattaaa agagttggat gtacatggtt tatgatgagt tgattggaga aatttattgg
    12541 gtgattggaa agattcagtc agacccagaa ttagaagaac aattgcgacg gttaaaattt
    12601 gatattcgca agaatggtgt caaagtcgct ggcgatccat atttaatgaa tgaggagacg
    12661 gatgctcgaa ttgaaataaa tcaagtaatt gcagaatttg agcggattgc ggaccaagca
    12721 aaagaaccag atattcgtca atacttgtta gaaatgaaag cagagttgga agtaaatggt
    12781 attacagatg agtaacctaa accaacacaa ctgactgtta ggcgctgacc gcggaggatt
    12841 cgcgaagcag aaatcctccg tggtgctgtg cctgttcatg gacggcgtta gccgaccatg
    12901 atgtctcacc cccgtatcta acaggggggt acaaaaacac aaaaaataag taagcaacca
    12961 ttaagaatgc ttgttgtatc aaagatagta agtaatttgt ataggtgtta actcatttga
    13021 aaaagttaac accttgaatt tggaggtgag aacttggcac aacggaattt agattatcgg
    13081 ctattaaaag accgacgcaa cgaatacggc gtttcacaaa ataaattggc tacggcttgt
    13141 ggacttagtc ggccgtactt aaatcagata gaaaatggtg gcgtaaccgc atctataaaa
    13201 accatgagga aaatttttga ccaactggaa agctttaatc ccgatttgcc tttgacgtta
    13261 ctgtttgact atgtaaggat tcgctttccc acaacggatg cacgaaaaat tattcaagaa
    13321 attctccatt tgaaatttga gtatatgctc catgaagatt atgcctttta ctcctatcaa
    13381 gaacagtatg tcatgggtga tattgtggtg atgttgtctc atgaagaaga taaaggtgtg
    13441 cttttagaat taaaggggcg aggttgccga caatttgaaa cttttttact cgctcaaaag
    13501 cgtagctggt acgacttttt cgaggattgt ctaaaggccg gtggcgtgat gaaacgcttg
    13561 gacttggcaa tcaatgatcg agtggggcta ttggatatac ctgagctaac gaaaaaatgt
    13621 cagaaggaag aatgtatctc cttgtttcgt acctttaaaa gttatcgttc cggggaactt
    13681 ttaaaggctg atgaaaagga cggcatgggc aataccttat atatcggcag tctcaagagt
    13741 gaggtttatt tctgtttgta tgaaaaagac tatgaacaat atatcaagtt agggattcca
    13801 ttagaccaaa ctgagacgaa aaatcgtttt gagattcgac tgaaaaatga ccgtgctttt
    13861 catgcgattc aagatttgtt aaaaggtcgt agcattgaga gcaccacgtt ttccattatc
    13921 aatcgttact tgcgatttgc ggataaagta gaaggtaaaa ggcgaaccaa ctggcccttg
    13981 aatgaacaat gggggcgttt tattggacgg aatcggaaag agattcaatt aacttccgag
    14041 ccaaaaccgt ataccattga acgaaccttg aattggctag gccgacaagt cgcccctacg
    14101 tggaaaatgg ctaaggagtt ggatcgattg aatcggacaa cctatatcca ggatatggta
    14161 cagaatgcac gcttatcaga ccgacataaa aagattttgg agcaacaaag tatggcaatt
    14221 gaaaatttga ttatatgaaa agaggaatcg tcatgggaat tgtaaaagat atttttttgg
    14281 tatttggtgg cagtatgctt ggtgttactg tgatgtgctt gatgaatgca agtgcagcag
    14341 cggatcgagc aatggaaaag aaaggaaagt gacaagatga atttcggaca aaatttatgt
    14401 gcgactcgtt ccttagtgaa aagctaaggc actaataaaa gaacgaaaaa gtttggttat
    14461 taggagaact gataatctga caggtggaat gatgaagtaa cgctctgaaa cgcctgccct
    14521 gagcctccga caagcattgt atgaggaaac tcagaatgtt tgaagctcgg taaagacggc
    14581 tgaaagatac cgtaatagtt gatgaacata tatcttctat cgaaaactat ccagaggtgg
    14641 gtagcgtatc caacaggtcg ggggtctata aaatatctat ggttagaatg ttctataagg
    14701 aactgacgaa acagcgaatg tacgggtcta aatttgaact ttgtagaaat gcaaaggtac
    14761 gagaaatcgt agctggtgtg gcaaagtaag aatataggtt atgaaatgcc atataacgtt
    14821 acaggcggta tcaagccagc aggcttatag ctagtgacct aaggatatat atgtatagat
    14881 aagattatcg gaacgaggaa aggtattggg ttctcatcaa gagaataaac tgacgaagaa
    14941 caataagcag ttgaactaat gctgaaaagc agagggcgaa cctaagaaat tcttgtaatg
    15001 agaatggagg aatgccctca agtcgtgtat atattagaaa agtattatgt tgtttcaata
    15061 aggattacga gtaagataaa aaagctcact caatttcaag taaaggagtg aagccttatg
    15121 cctaaaaata aaaatgaaac attgtgtgta gaagatttaa gacacgctga atattacgaa
    15181 atgcaaaaca cctttgatga tttatatgcc aaaagtaaga atggagatat tttcactcat
    15241 cttatggata ttatcctatc gagagaaaat atattactgg catatcgaaa tatcaaagca
    15301 aatgcaggca gtaaaacagc aggaacagac ggtacaataa ttaaagatat tggcaaattg
    15361 ccagcagaaa cagttgtcaa aaaagtaaga tacattgttg caggcagtcc acatgggtat
    15421 cgacctaagc cagtgaggcg aaaagaaata ccaaaaccaa atggtaaaac cagacctcta
    15481 ggtattcctt gtatgtggga caggcttata caacaatgta taaaacaagt attagaacca
    15541 atctgcgaag ctaaatttag tgaaaacagc tatggtttta gacctaatag gtcagttgaa
    15601 aatgcaataa aagcaaccta taaccgccta caaatcagcc agctacatta tgtgattgaa
    15661 tttgatatta agggtttctt tgacaacgtg aaccattcca agctgataaa acagatatgg
    15721 gctatgggaa tacgggacaa gcacttgata ttcattttaa aaaggatatt aaaagctcca
    15781 atcaaaatga ctaacggtac aataacatat ccagagaaag gaactccgca agggggaata
    15841 atatccccac tactagcaaa cattgttctt aatgagttag accattgggt agaaagccaa
    15901 tggcaggaaa atcctgttac aaaaaactat gtggttcata tcaataaaag cggtagccca
    15961 tgtaaaagca acgcttacaa agaaatgaag aaaacaaagt taaaggaaat gtatatggtt
    16021 cgttatgcag atgattttag agttttctgc cgctacaagg aaagtgctga aaaagccaaa
    16081 atagcaatca ctcaatggat tgaacaacga ttaaaattgg aagtatcaca agaaaaaaca
    16141 agaattgtaa atgttagaaa aagatactcc gactttcttg gtttcaagat taaaatgata
    16201 ccgagaagaa agaagttagt tgttaaatca catataagtg ataaacaatt caaaaaccaa
    16261 aaagacaagc tggtatcaca ggcaaagaaa attgcttctc cctcaaaagg gaaaacggaa
    16321 ttagaagaaa caagacttta taattcaatg gtattaggta tgcaaaatta ttatcagatt
    16381 gcaacgaatg taaatctgga ttgtatgaaa ctgaatagag ctgtaatggc tatcttcact
    16441 aacagattag gtacaaacaa aaggggcaga ttgagaaaaa caggcagaca acttacgaca
    16501 aaggaggcaa caagatatgg taaatctaaa atgcttcggt atgttgctgg cgagggcgaa
    16561 cctattttcc ctgttggata tatccaacac agaaaaccaa tggcaaaggt gtatttagcg
    16621 aattgctaca ctctagaggg aagaaaattt atccacacca atctaagtgt tgataaatta
    16681 ctgatgtacc agttaatgaa attatctttg gaaaatcgca caatagaata cgcagacaat
    16741 cgaatttcgc tattttctgc tcaacatgga aaatgtgcta taactctgga agaatttcaa
    16801 caagctaatg aaatacattg tcatcatatt gtaccaactg gagctggtgg aaatgacgat
    16861 tataaaaatc ttattttggt aaaagaggcg gtacaccgtc taatacacgc taaaacagaa
    16921 gaaacaatac aaaaatatat agctctattg aagctaaata aagtccagct cataagacta
    16981 aataaatata gagtattagc tggcaatcaa gaattaaact tgatataggt ctactacata
    17041 aatacttttc aatataaaca cgatggaacg cggagtgcgg tgaaagtcgc acgctccgtg
    17101 tgaatggggg gaaaagtttg agatatttat aatatcagga acttacctat ccatatataa
    17161 ctggtttcta tcaaacgctc aatcactggt gcttttagca atcgttgtga ttggcttgta
    17221 tcttggcttc aagcgtgagt ttagcaaact gattggcttt ttaattattg cgattattgc
    17281 ggttggctta gtcttcaacg ctgctggagt aaaagacatt ttactagagc tattcaatcg
    17341 cattattggt gcttaaataa aaccgttctt ttgtggaata taagtggttt tcttatgttc
    17401 cgcaaaggaa tggtacacca aacgaagtgc ggtagggatt tttgaatctc tacaaagaaa
    17461 ggacgtgaat atatggacga tatgcaagtc tatattgcga atttaggcaa atacaatgag
    17521 ggcgaattgg tcggtgcgtg gtttaccttt cccattgact ttgaggaagt caaagagaaa
    17581 atcggcttga atgatgaata tgaggaatac gccattcatg attacgagtt accctttacg
    17641 gttgacgaat acacttctat tagcgaactc aatcgcctat gggaaatggt atcggagttg
    17701 ccagaagaac tacaatcgga gctatctgct ctgctcactc atttttcaag tattgaagaa
    17761 ctaagcgaac atcaagaaga tattatcatt cattccgatt gtgatgatat gtctgacgtg
    17821 gcacgctact acattgaaga aacgggtgct ttaggcgaag tgccagccag tcttcaaaac
    17881 tatattgatt atgaatccta tggtcgggat ttagaccttt cgggaacgtt tatctcaacc
    17941 aatcacggga tttttgaaat cgtctattaa atctgtcggt acattcttac tggcagattt
    18001 tcttgtttac ggggtagtca aaagactatc cccattttta tgaaaggatt gattacatga
    18061 agaaaatacg aagctatacc agtatctggt ctgttgagaa agtgttgtac tccatcaatg
    18121 actttagact tccgtttccc ataaccttta cgcaaatgac atggtttgtc gtgtcactct
    18181 ttgcagttat gatacttggc aacttgcccc ctctttctat gatagaggga gcatttctca
    18241 aatactttgg gattcctgtg gctttcacat ggtttatgtc tacaaaaacc tttgatggta
    18301 aaaagcctta tggatttttg aagtctgtca ttgcttatgc actgcgacga aagctgacct
    18361 atgcaggaaa aaaagtaacg cttggcagaa accagccaca agaagccatt acagcagtta
    18421 ggagtgaatt ttatggcata tccaattaaa tatattgaaa acaatctggt ctggaataaa
    18481 gatggggaat gttacgctta ctatgagctt gttccctaca attactcatt tctaagtccg
    18541 gaacagaaaa tacaagtaca tgattctttc agacagctta tcgcacaaaa tcgtgatggc
    18601 aagattcatg ctttacaaat cagtacagaa tccagcatac tttctgcaca agaacgttcc
    18661 aaaaatgaag tcactggaaa gctcaaaacg gttgcctatg acaaaatcga ccaacagaca
    18721 gacgctttaa tatccatgat tggcgaaaat caagtggact accgtttctt tatcggtttt
    18781 aagttgcttc tcaacgatca ggagttttct atgaaaagtc ttaccgttga agcaaaaaat
    18841 gcttttactg attttgtcta tgatgtgaac cataagctga tgggcgattt tgttagtatg
    18901 agtaatgatg aaatcctgcg ttttcagaag atggaaaagc ttttagaaaa taaaatctcc
    18961 cgtcgtttca aaatccgaag attagataag gatgacttcg gctatctgat tgaacacctt
    19021 tacggacaga caggcactgc ctatgaagag tatgagtatc ctctatcaaa gaaaaagctg
    19081 gaacatgaaa cactgattaa aacctatgac ctcattaagc ctactcgctg tctggtggaa
    19141 gaaaaacagc gatatttgaa aatccagcag gaagacgaaa ccgcctatgt agcttacttt
    19201 accatcaaca gtattgtcgg cgaactggac ttcccgtcct ctgaaatctt ctactaccag
    19261 caacagcaat ttacctttcc gattgatacg tcaatgaatg tggaaattgt agcgaatcgt
    19321 aaagccctat ctactgtccg caataaaaag aaagaactga aagacttgga taaccacgct
    19381 tggcaaagtg ataatgaaac cagctctaat gtggcggaag ctctggaaag tgtgaacgag
    19441 ctggaaacca atttagacca aagcaaggaa tctatgtata agctgtccta tgttgtaagg
    19501 gtatcagcaa atgaccttga cgaacttaaa cgtcgttgta atgaagtgaa agatttctat
    19561 gatgatttga gcgtgaaact ggtacgacct tttggggata tgctgggctt acatgaagaa
    19621 tttttacctg ccagcaaaag atatatgaat gactatattc aatacgtgac ctctgatttc
    19681 ctcgctggtt taggttttgg tgctactcaa atgctgggtg aaaatgaggg gatttatgtt
    19741 ggctacagct tagatactgg acgcaatgtc tatctgaaac ctgctcttgc cagtcaaggg
    19801 gttaagggtt cagtaaccaa tgcgttagcg tctgcctttg tcggttcgct gggtggtggt
    19861 aaatcctttg cgaataacct tatcgtctat tatgcagtgc tttatggggc acaagcagtg
    19921 attgtagacc caaaagcaga acgtggcaga tggaaagaaa ccttgccaga gatttcccat
    19981 gaaatcaata tcgtcaatct gacttctgat gagaaaaaca aaggcttact tgacccttat
    20041 gtaattatga aaaataccaa agattctgaa tcactggcta ttgatatttt gacattcctt
    20101 acggggattt cctctcgtga tggggaacgc ttcccaatcc ttagaaaagc cattcgtgca
    20161 gtaaccaata gtgaagtgcg agggttgatg aaagtgattg aagaattacg ggttgagaat
    20221 acgccactaa gtaccagcat agccgaccat atcgaaagtt ttacagacta tgactttgcc
    20281 catctgcttt ttagtgatgg ttatgtagag cagtctatca gccttgaaaa acaactgaac
    20341 attatacagg ttgccgactt ggtacttcct gacaaggaaa cttcctttga ggaatatacc
    20401 acaatggagt tactatcggt agcaatgctg attgtcatta gtacctttgc gttggacttt
    20461 atccatacag accgaagtat tttcaagatt gtagacttag acgaagcatg gagcttctta
    20521 caggtggcac aagggaaaac actatctatg aagctggttc gtgctggtcg tgctatgaac
    20581 gctggggtat atttcgtgac ccaaaataca gatgacctct tagatgaaaa actgaaaaat
    20641 aacctcggct taaaatttgc ctttcgttcc actgacctta acgagattaa aaaaaccctt
    20701 gccttttttg gtgtagaccc agaggacgaa aacaatcaga agcgattgcg tgatttggaa
    20761 aacgggcaat gccttatcag tgatttatat ggtcgtgtcg gtgtgataca gttccaccct
    20821 gtatttgaag aactgctcca tgcctttgat accagaccac ctgtgcgaaa agaggtgtaa
    20881 atgtgaaacc atcaatatta aacagaataa aatcaaactg gacgctgaaa cgtctaggta
    20941 aagtggcaat gacagtggct ttcacacttg tgattgccat ttttctttta gccatgctgg
    21001 gaacggtggt tcaagctgcg ggcttggtag atgatacggt caatgtggca aatgaataca
    21061 gccgataccc acttgaaaac tatcaactgg atttttatgt ggataatagc tggggctggc
    21121 taccgtggaa ctggtcggac ggaattggaa aacaagtcat gtatggacta tatgccatta
    21181 ccaattttat ttggacaatc agtctttatg tttccaatgc gacgggttac ttagtacagg
    21241 aagcctattc cttagacttc atttccgcta cagcagattc cattggcaag aatatgcaga
    21301 cccttgcagg ggttacgcct agtggactat caacagaggg tttctatgtt ggattccttt
    21361 tactcttgat tttggttctt ggggtttatg ttgcctatac aggactgata aagagagaaa
    21421 ccacaaaggc aattcatgcc ctcatgaatt ttgtgctggt ttttatccta tcggcttcct
    21481 ttattgccta cgctcccgac tacattaaaa aaatcaatga cttttcatca gacatcagca
    21541 acgctagttt gtcactgggt acaaaaattg tcatgcccca ttcagatagt caaggcaagg
    21601 acagtgtgga cttgatacgt gacagcctat tttccataca ggttcagcaa ccgtggctac
    21661 tgcttcaata taacagttca gacattgaaa gtattggtat tgaccgtgtg gaaagcctgc
    21721 tctcaaccag tccagattcc aacaatgggg aagacagaga aaaaattgtt gcggaagaaa
    21781 ttgaagatag aagcaatacc aatatgacga ttacaaagac gattaaccgt ttaggtacag
    21841 tcttcttcct atttgtcttc aatattggga tttccatatt tgtattccta ttaacaggaa
    21901 tcatgatttt ctcgcaggta ctttttatca tctatgctat gtttctgcct gtgagcttta
    21961 ttttaagcat gattccatca tttgatggta tgtcaaaacg agccataaca aagctcttta
    22021 ataccatttt gacacgagct ggaatcacat tgattattac gacagcattt agtatttcaa
    22081 ccatgctcta taccttatcg gctggttatc cgttcttttt gattgctttt ctacagattg
    22141 tgacctttgc aggaatctac tttaagctgg gcgatttaat gagtatgttt tctctacaga
    22201 gtaacgattc tcaaagtgtg ggaagtcgta tgatgagaaa acctcgtatg cttatgcacg
    22261 ctcacatgca ccgtctacag cggaaacttg gacgctccgt gactgcttta ggggctgggt
    22321 ctgccattgc cagtgctacg ggtaaaaaag gacagtcggg ttcgggaagt tctgcgagta
    22381 cacaagcaga tcactctcga ccaaacgggc aggaaaaatc aacactagga aagcgtatcg
    22441 gtcaaaccat cggtacagta gctgatacca aagacagaat ggtagatact gctggtaatt
    22501 tgaaagaaca ggttaaggat ttgccgacca atgcaagata tgcagtatat caaggaaaat
    22561 ctaaagtgac ggataatgtc catgatttaa ccagaagtat ttctcaaacc aaagcggata
    22621 aagccagtgg acgcaagaaa cagcaggaac aaagacggaa aaccattgcg gagcgtcgct
    22681 ctgaaatgga acaggtcaaa cagaaaaaac agccagcttc ttccgttcat gaaagaccag
    22741 ccactaagca agaacaatct catgatggac agacttcaag gcaaacaact gtacaggctt
    22801 cctataggga atctcaacaa gccaaacaag agcgtccaac agttaagtcc gattcttcaa
    22861 gtttaaaaac ggaacgtcaa agtaatacag ttcaagaaag aacggttcaa aagccagtaa
    22921 cttcaactgc accaacagat agagcttcac aacgttcaat cacaaaagaa cgtcagtcta
    22981 ctgttcaaag agtaccacta caaaatacaa aaagtagacc accaatcaaa accgccacca
    23041 ttaagaaagg cagtaagaaa ccatgaagct gaaaacttta gtgattggtg gttcgggatt
    23101 attcttgatg gtcttctcac tgcttctgtt tgtcgccatt ttattttcag atgaacaaga
    23161 tggcggtttt tccaacattc actatggcgg tgtggatgtt tccgcagaag tgctggctca
    23221 taagcctatg gtagaaaaag ttgccaaaga atatggaatt gaagaatacg tcaatatact
    23281 tcttgcgatt atacaggtgg aatcgggtgg tacagcagaa gatgttatgc agtcctcgga
    23341 atccctcggt cttccaccta attcattgag tacagaagaa tccattaagc aaggtgtgaa
    23401 gtatttcagt gagttattag ccagtagcga aaggcttggt gtagatttag aatcggttat
    23461 ccagtcctac aattatggtg gtggtttctt aggatatgtg gctaatcgtg ggaataaata
    23521 tacctttgaa ctggctcaaa gtttctcaaa agagtattcg ggtgacgaaa aagtgtctta
    23581 ccccaatccc atagccatac ctatcaatgg gggctggcga tacaattacg ggaatatgtt
    23641 ttatgtgcaa ctggtaacgc agtatcttgt cacaacagag tttgatgatg atacagtaca
    23701 agccatcatg gacgaagctc tgaaatttga aggctggaag tatgtctatg gcggagcttc
    23761 cccgactacc tcttttgatt gtagcggact gacacaatgg acgtatggaa aagctggaat
    23821 caacttacca cgaacagcac aacagcaata tgatgtaacc cggcatatcc cattattgga
    23881 agcaaaagcc ggcgatttgg ttttctttca ttctacctat aacgctggct cttatattac
    23941 tcatatcggg atttatttag gcgataaccg tatgtttcat gcaggcgacc ctatcggtta
    24001 tgccgactta acaagctctt actggcaaca gcatttagtg ggagcaggac gaatcaaaca
    24061 ataagaaagg acgattcaat gatgaaattc ggaaaaaatc agaatacaga aaaacagaca
    24121 ccaaaggaaa agaaacctcg tgtctataag gtcaatcctc gtaaaaaggt tgtgattgtc
    24181 ctatgggtac ttttaggact tagtttcagc tttgcgatat tcaagcactt tacagccatt
    24241 gatacccata ccattcatga aaccaccatc atagaaaagg aatatgtcga tactcatcat
    24301 gtggaaaatt ttgtagagaa ctttgcgaaa gtctactatt catgggaact aaacgacaag
    24361 tccattgata atcgcataga aaacctgaaa gcatatctga cagaggaact tcaagctctg
    24421 aatgtcgata cagttcgtaa agatattcct gtatcgtctt ctgtaaaagg atttcagata
    24481 tggacggtag aagcagatgg cgacaatcaa tttgatgtaa cctacagtgt agaccaactc
    24541 attacagagg gggaaaatac aaagaccgtc cactctgctt atatagtgag tgtctatgta
    24601 gacagtactg gaaatatggt cctcattaag aatccgacca ttactagcat accaaagaaa
    24661 tcagactata aaccaaaagc tattgaaagt gagggtacgg ttgattccat tacaaccaat
    24721 gaaatcaatg agtttttaac gacgttcttc aagctctatc ctacagcgac agccagtgaa
    24781 ctttcctact atgtgaatga cgggatatta aaaccaatcg gaaaagagta catctttcaa
    24841 gaactggtaa atcctattta caatcgtaag gataatcaag tcacggtatc gctgacagtg
    24901 gagtatatcg accagcagac caaagcaacg caggtatctc aatttgattt ggtacttgaa
    24961 aagaacggga gtaattggaa gattatagaa taacaaatat tggtacatta ttacagctat
    25021 tttgtaatca cgtactctct ttgataaaaa attggagatt cctttacaaa tatgctctta
    25081 cgtgctatta tttaagtatc tatttaaaag gagttaataa atatgcggca aggtattctt
    25141 aaataaactg tcaatttgat agtgggaaca aataattgga tgtccttttt taggagggct
    25201 tagttttttg cacccagttt aagaatacct ttatcatgtg attctaaagt atccggagaa
    25261 tatctgtatg ctttgtatgc ctatggttat gcataaaaat cccagtgata agagtattta
    25321 tcactgggat ttttatgccc ttttgggctt ttgaatggag gaaaatcaca tgaaaattat
    25381 taatattgga gttttagctc atgttgatgc gggaaaaact accttaacag aaagcttatt
    25441 atataacagt ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga
    25501 taatacgctt ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca
    25561 gtgggaaaat acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga
    25621 agtatatcgt tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg
    25681 cgtacaagca caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat
    25741 cttttttatc aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat
    25801 taaagagaaa ctttctgccg aaattgtaat caaacagaag gtagaactgt atcctaatat
    25861 gtgtgtgacg aactttaccg aatctgaaca atgggatacg gtaatagagg gaaacgatga
    25921 ccttttagag aaatatatgt ccggtaaatc attagaagca ttggaactcg aacaagagga
    25981 aagcataaga tttcagaatt gctccttgta ccctgtttat catggaagcg caaaaagcaa
    26041 catagggatt gagcagctta tagaagtgat aacgaataaa ttttattcat caacatacag
    26101 aaagaagtct gaactttgcg gaaatgtctt caaaattgaa tattcggaag aaagacaacg
    26161 tcttgcatat gtacgccttt atggcggaat cctgcatttg cgggattcgg ttagaatatc
    26221 ggaaaaggaa aaaataaaaa ttacagaaat gtatacttca ataaatggtg aattatgtaa
    26281 aattgataag gcttattccg gggaaattgt tattttgcaa aatgagtttt tgaagctaaa
    26341 tagtgttctt ggagatacaa agctattgcc acagagagag agaattgaaa atccgctccc
    26401 tctgctgcaa acaactgttg aaccgagcaa acctcaacaa agggaaatgt tacttgatgc
    26461 acttttagaa atctccgaca gtgacccgct tctacaatat tatgtggatt ctacgacaca
    26521 tgaaattata ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca
    26581 agaaaagtat catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc
    26641 gttaaaaaat gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc
    26701 cattggttta tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt
    26761 ttctcttgga tacttaaatc aatcatttca aaatgcagtt atggaaggga tacgctatgg
    26821 ttgcgaacaa ggattatatg gttggaatgt gacggattgt aaaatctgtt ttaagtatgg
    26881 cttatactat agccctgtta gtaccccagc agattttcgg atgcttgctc ctattgtatt
    26941 ggaacaagtc ttaaaaaaag ctggaacaga attgttagag ccatatctta gttttaaaat
    27001 ttatgcgcca caggaatatc tttcacgagc atacaacgat gctcctaaat attgtgcgaa
    27061 catcgtagac actcaattga aaaataatga ggtcattctt agtggagaaa tccctgctcg
    27121 gtgtattcaa gaatatcgta gtgatttaac tttctttaca aatggacgta gtgtttgttt
    27181 aacagagtta aaagggtacc atgttactac cggtgaacct gtttgccagc cccgtcgtcc
    27241 aaatagtcgg atagataaag tacgatatat gttcaataaa ataacttagt gtattttatg
    27301 ttgttatata aatatggttt cttgttaaat aagatgaaat attttttaat aaagatttga
    27361 attaaagtgt aaaggaggaa ttaattatga agtgtaatga atgtaacagg gttaaataca
    27421 accaaatttt aatttggctt tgcgttttat ctttttttag cgtattaaat gaaatggttt
    27481 tgaacgtctc attacctgat attgcaaatg attttaataa accacctgcg agtacaaact
    27541 gggtgaacac agcctttatg ttaacctttt ccattggaac agctgtatat ggaaagctat
    27601 ctgatcaatt aggcatcaaa aggttactcc tatttggaat tataataaat tgtttcgggt
    27661 cggtaattgg gtttgttggc cattctttct tttccttact tattatggct cgttttattc
    27721 aaggggctgg tgcagctgca tttccagcac tcgtaatggt tgtagttgcg cgctatattc
    27781 caaaggaaaa taggggtaaa gcatttggtc ttattggatc gatagtagcc atgggagaag
    27841 gagtcggtcc agcgattggt ggaatgatag cccattatat tcattggtcc tatcttctac
    27901 tcattcctat gataacaatt atcactgttc cgtttcttat gaaattatta aagaaagaag
    27961 taaggataaa aggtcatttt gatatcaaag gaattatact aatgtctgta ggcattgtat
    28021 tttttatgtt gtttacaaca tcatatagca tttcttttct tatcgttagc gtgctgtcat
    28081 tcctgatatt tgtaaaacat atcaggaaag taacagatcc ttttgttgat cccggattag
    28141 ggaaaaatat accttttatg attggagttc tttgtggggg aattatattt ggaacagtag
    28201 cagggtttgt ctctatggtt ccttatatga tgaaagatgt tcaccagcta agtactgccg
    28261 aaatcggaag tgtaattatt ttccctggaa caatgagtgt cattattttc ggctacattg
    28321 gtgggatact tgttgataga agaggtcctt tatacgtgtt aaacatcgga gttacatttc
    28381 tttctgttag ctttttaact gcttcctttc ttttagaaac aacatcatgg ttcatgacaa
    28441 ttataatcgt atttgtttta ggtgggcttt cgttcaccaa aacagttata tcaacaattg
    28501 tttcaagtag cttgaaacag caggaagctg gtgctggaat gagtttgctt aactttacca
    28561 gctttttatc agagggaaca ggtattgcaa ttgtaggtgg tttattatcc atacccttac
    28621 ttgatcaaag gttgttacct atggaagttg atcagtcaac ttatctgtat agtaatttgt
    28681 tattactttt ttcaggaatc attgtcatta gttggctggt taccttgaat gtatataaac
    28741 attctcaaag ggatttctaa atcgttaagg gatcaacttt gggagagagt tcaaaattga
    28801 tccttttttt ataacaggaa ttcaaatctt tttgttccat taaagggcgc gattgctgaa
    28861 taaaagatac gagagacctc tcttgtatct tttttatttt gagtggtttt gtccgttaca
    28921 ctagaaaacc gaaagacaat aaaaatttta ttcttgctga gtctggcttt cggtaagcta
    28981 gacaaaacgg acaaaataaa aattggcaag ggtttaaagg tggagatttt ttgagtgatc
    29041 ttctcaaaaa atactacctg tcccttgctg atttttaaac gagcacgaga gcaaaacccc
    29101 cctttgctga ggtggcagag ggcaggtttt tttgtttctt ttttctcgta aaaaaaagaa
    29161 aggtcttaaa ggttttatgg ttttggtcgg cactgccgac agcctcgcag agcacacact
    29221 ttatgaatat aaagtatagt gtgttatact ttacttggaa gtggttgccg gaaagagcga
    29281 aaatgcctca catttgtgcc acctaaaaag gagcgattta catatgagtt atgcagtttg
    29341 tagaatgcaa aaagtgaaat cagctggact aaaaggcatg caatttcata atcaaagaga
    29401 gcgaaaaagt agaacgaatg atgatattga ccatgagcga acacgtgaaa attatgattt
    29461 gaaaaatgat aaaaatattg attacaacga acgtgtcaaa gaaattattg aatcacaaaa
    29521 aacaggtaca agaaaaacga ggaaagatgc tgttcttgta aatgagttgc tagtaacatc
    29581 tgaccgagat ttttttgagc aactggatcc aggagaacaa aaacgatttt ttgaggaaag
    29641 ttataaatta ttttccgaac gatatggcaa gcaaaatatt gcttatgcaa cagttcataa
    29701 tgatgagcaa acccctcaca tgcatttagg tgttgtgcct atgcgtgatg gaaaactgca
    29761 aggaaaaaat gtgtttaatc gtcaagaact gttatggcta caagataaat tccccgagca
    29821 tatgaaaaaa cagggttttg agttgaagcg tggtgaacgt ggctctgacc gtaaacatat
    29881 tgagacagct aaatttaaaa aacaaacttt ggaaaaagag attgattttc tagaaaaaaa
    29941 tttagcagtt aaaaaagatg aatggactgc ttatagcgat aaagttaaat cagatttaga
    30001 agtaccagcg aaacgacaca tgaaaagtgt tgaagtgcca acgggtgaaa agtccatgtt
    30061 tggtttggga aaagaaataa tgaaaacaga aaagaaacca accaaaaatg ttgttatatc
    30121 ggagcgtgat t
//
LOCUS       DAEGIQ010000026        29846 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000026 DAEGIQ010000000
VERSION     DAEGIQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.026"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            274..1143
                     /locus_tag="KMT45_001039"
     CDS             274..1143
                     /locus_tag="KMT45_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBE7004514.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1352..2377
                     /locus_tag="KMT45_001040"
     CDS             1352..2377
                     /locus_tag="KMT45_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBE7004515.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2416..3366
                     /gene="manA"
                     /locus_tag="KMT45_001041"
     CDS             2416..3366
                     /gene="manA"
                     /locus_tag="KMT45_001041"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBE7004516.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3460..5163
                     /locus_tag="KMT45_001042"
     CDS             3460..5163
                     /locus_tag="KMT45_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBE7004517.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5165..7198
                     /locus_tag="KMT45_001043"
     CDS             5165..7198
                     /locus_tag="KMT45_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBE7004518.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSGRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7209..7649
                     /locus_tag="KMT45_001044"
     CDS             7209..7649
                     /locus_tag="KMT45_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE7004519.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7849..8976)
                     /locus_tag="KMT45_001045"
     CDS             complement(7849..8976)
                     /locus_tag="KMT45_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004520.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8979..9656)
                     /locus_tag="KMT45_001046"
     CDS             complement(8979..9656)
                     /locus_tag="KMT45_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004521.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9920..11686
                     /locus_tag="KMT45_001047"
     CDS             9920..11686
                     /locus_tag="KMT45_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBE7004522.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11701..12138
                     /locus_tag="KMT45_001048"
     CDS             11701..12138
                     /locus_tag="KMT45_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE7004523.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12151..13302
                     /locus_tag="KMT45_001049"
     CDS             12151..13302
                     /locus_tag="KMT45_001049"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBE7004524.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13364..14062)
                     /locus_tag="KMT45_001050"
     CDS             complement(13364..14062)
                     /locus_tag="KMT45_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBE7004525.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14055..15407)
                     /locus_tag="KMT45_001051"
     CDS             complement(14055..15407)
                     /locus_tag="KMT45_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBE7004526.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15630..17030)
                     /locus_tag="KMT45_001052"
     CDS             complement(15630..17030)
                     /locus_tag="KMT45_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBE7004527.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17191..17928
                     /locus_tag="KMT45_001053"
     CDS             17191..17928
                     /locus_tag="KMT45_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBE7004528.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18053..18532
                     /locus_tag="KMT45_001054"
     CDS             18053..18532
                     /locus_tag="KMT45_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBE7004529.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18529..19296
                     /locus_tag="KMT45_001055"
     CDS             18529..19296
                     /locus_tag="KMT45_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004530.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19526..20095
                     /gene="lepB"
                     /locus_tag="KMT45_001056"
     CDS             19526..20095
                     /gene="lepB"
                     /locus_tag="KMT45_001056"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBE7004531.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20199..20801)
                     /locus_tag="KMT45_001057"
     CDS             complement(20199..20801)
                     /locus_tag="KMT45_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBE7004532.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20907..21376
                     /locus_tag="KMT45_001058"
                     /pseudo
     CDS             20907..21376
                     /locus_tag="KMT45_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21482..22066
                     /locus_tag="KMT45_001059"
     CDS             21482..22066
                     /locus_tag="KMT45_001059"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBE7004533.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22069..23142
                     /gene="prfA"
                     /locus_tag="KMT45_001060"
     CDS             22069..23142
                     /gene="prfA"
                     /locus_tag="KMT45_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBE7004534.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23135..23974
                     /gene="prmC"
                     /locus_tag="KMT45_001061"
     CDS             23135..23974
                     /gene="prmC"
                     /locus_tag="KMT45_001061"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBE7004535.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23967..24146
                     /locus_tag="KMT45_001062"
     CDS             23967..24146
                     /locus_tag="KMT45_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004536.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24168..25193
                     /locus_tag="KMT45_001063"
     CDS             24168..25193
                     /locus_tag="KMT45_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBE7004537.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25368..26612
                     /locus_tag="KMT45_001064"
     CDS             25368..26612
                     /locus_tag="KMT45_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBE7004538.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26765..27712
                     /locus_tag="KMT45_001065"
     CDS             26765..27712
                     /locus_tag="KMT45_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE7004539.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27846..28475
                     /gene="upp"
                     /locus_tag="KMT45_001066"
     CDS             27846..28475
                     /gene="upp"
                     /locus_tag="KMT45_001066"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBE7004540.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28614..28856)
                     /locus_tag="KMT45_001067"
     CDS             complement(28614..28856)
                     /locus_tag="KMT45_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004541.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28977..29049
                     /locus_tag="KMT45_001068"
     tRNA            28977..29049
                     /locus_tag="KMT45_001068"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29010..29012,aa:Thr,seq:cgt)
     gene            complement(29168..29806)
                     /locus_tag="KMT45_001069"
     CDS             complement(29168..29806)
                     /locus_tag="KMT45_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBE7004542.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10147 a         5271 c         6075 g         8353 t
ORIGIN      
        1 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
       61 taggaggagc tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca
      121 acctcttttt ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt
      181 tctcacattt tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta
      241 aaataaagct tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc
      301 acctatttaa aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac
      361 aagaacgttg ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca
      421 gaagaagctg gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg
      481 ggggtgatca aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca
      541 gatatccttt gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat
      601 ggacgtatgg ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa
      661 gagaataaga acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga
      721 aaatctgaag aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta
      781 aatgttacta acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta
      841 cataatgaag aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca
      901 gaagctagcg gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg
      961 aaattcatga tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca
     1021 gttgatttct tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc
     1081 gaatggtggt tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct
     1141 taactttttc tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat
     1201 cgcttattct agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg
     1261 cgcagttttt ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg
     1321 taagaatctg atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt
     1381 gaaaaagatg gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca
     1441 agtagaaaca tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga
     1501 agatctgttg tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt
     1561 acgtctatta gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga
     1621 atttgaaaat cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac
     1681 atggatacca aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct
     1741 agaaccaggt gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct
     1801 tgggttgatg ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt
     1861 attggaacca ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt
     1921 tcccacagaa caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc
     1981 aagagttttg gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat
     2041 tacttttgtc caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga
     2101 agagacgatt gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt
     2161 aggatgggac ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta
     2221 tacaggtacg tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa
     2281 tcgcgtacat cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga
     2341 aaactttttg aaagagaaag cggtattacc gaaataatgc tataatagct atgaaaaatg
     2401 agaggagttt gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt
     2461 tggggaggaa gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga
     2521 gaagattggg cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc
     2581 aaagggaaaa gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca
     2641 gaaccagtgt ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa
     2701 gtacatccag atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc
     2761 tggtatatca tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca
     2821 agagaagaac tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta
     2881 ccagttaaaa aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa
     2941 ggaatcatga ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac
     3001 gacagaaaag atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta
     3061 acaactgtcc ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca
     3121 tcgatcgtca cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga
     3181 atcacatctt tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga
     3241 gaccttgtcg ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca
     3301 aatggcgttt ctgagtgggc ggtccaaggt gaactagcaa tcattgcgtc tgaaccaggc
     3361 aaatagttcc cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat
     3421 aaaatccgtc catcaataaa tagaagtctg gtgaaaaaga tggaatacaa atggaaagca
     3481 aacaacagca gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa
     3541 atgatcatgc caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa
     3601 ggtgtgtctg gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg
     3661 cttttaccca tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca
     3721 gtcgccggtg caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta
     3781 ggagcgatga ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc
     3841 catgtgaaat tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact
     3901 ggtagcgtta tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac
     3961 ttgttgctcg cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt
     4021 ctgatcttag aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg
     4081 acacctttag gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag
     4141 gcaaatcctg gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag
     4201 gaaaaagtaa atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc
     4261 tattttccat ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca
     4321 agcggcacgt tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg
     4381 tcactactcg tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg
     4441 atagcaatca gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat
     4501 aaagaaaaag agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt
     4561 cattccgtca tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg
     4621 ctgaaaaaga aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat
     4681 gaattgaata accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca
     4741 agagaaaaag tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc
     4801 ttgctgtcac gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata
     4861 gcagatatca cagaacaaaa aacgtcgcct gctaatattg tatttttgta taaaaacaac
     4921 agacgcggct cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa
     4981 aaacagtttg ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt
     5041 tatatcgcac aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta
     5101 gttctagttg aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata
     5161 taagatgtat ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc
     5221 acctgtatct atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg
     5281 cgaattagat aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc
     5341 tagaggctca tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac
     5401 agatgggaaa agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt
     5461 gttactgacg acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa
     5521 taccactttt tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga
     5581 aatcattcgg aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac
     5641 agcgaatgta ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga
     5701 tgcatcactg aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa
     5761 agtaatcggg gaagaattga tccaaaaaaa gaccaatcta agtggtagaa agcttgaaca
     5821 tttggttttg atgctgacat tgacgatgga gcgtgtgacg aaaaatcatt tactgacaaa
     5881 tgagacatat aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc
     5941 acggatcgct tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg
     6001 attattaaat gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac
     6061 acagcttgct tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca
     6121 tttttatgaa gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc
     6181 aagagccgtt ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca
     6241 atacgaggaa gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa
     6301 attatccgaa gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag
     6361 tccgaaagaa agcaatatca gtattgcagg attttcgcca agtggattag cttctactag
     6421 catgttggaa ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg
     6481 gattgctgat ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc
     6541 tattttacca ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga
     6601 agtcaagcag ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac
     6661 gtataacagc aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat
     6721 ggatcaaatc aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac
     6781 gatttccgaa acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga
     6841 agaggtggtt catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca
     6901 tacaaatatg gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat
     6961 tttcaatttg aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc
     7021 ccgtatgtta gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg
     7081 aaagatcagt ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa
     7141 taaagaaatc gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag
     7201 gtgaaaagat gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt
     7261 ggaaagaagc gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg
     7321 catatatcca atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg
     7381 tagccttgcc acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag
     7441 tccaagtgcc tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct
     7501 tatttgtagt ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt
     7561 tctgttcaga catcgaccaa gtaatggcgc tcagtgatgc ggaaaccatt gaagaagtcc
     7621 aaatgatttt tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc
     7681 atcaagtaat aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct
     7741 tattactgcg gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat
     7801 aggggacggt gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt
     7861 ttttatatag atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt
     7921 caagatacca ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc
     7981 tatgatcaca ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat
     8041 ccatccaatc gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa
     8101 aagattgact agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa
     8161 gagcacccac aacaacagga acagaaagct ctggaaaaga tactcataca tcggcaggat
     8221 tcgtatctcc tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt
     8281 gattattact agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc
     8341 ataataaatg gtaaaacgga tgctatgttt ctttaccata ctatccggta atagattgac
     8401 tagagagatt acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt
     8461 tatacataaa atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc
     8521 taaagagaga tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat
     8581 ataacggagc aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc
     8641 agaaatcagt cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg
     8701 gacattccct gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa
     8761 actttccaca aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat
     8821 ctcttcatag ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa
     8881 aagtgggaat gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt
     8941 tagtaagaga ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga
     9001 taaatagcta aaatctcttc catatttgag gattctgtga cgatttctcc attttttaga
     9061 aaacatgctt tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc
     9121 gtcttcccct ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg
     9181 tctaatccgt tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca
     9241 gcaagcaaga catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac
     9301 tcagtcatct tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat
     9361 ttgatcaaag tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct
     9421 agaaaaaaga aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac
     9481 aattgatcat aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca
     9541 aatccgttcg gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt
     9601 cgaatcacac gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat
     9661 aggcctccaa tataaaattt aataacataa tagaatggaa aacatctaaa ttaaatgcta
     9721 acaaaagtta ttgtttgcat attataaaaa aattaaaata acagttttat ttaaaattga
     9781 agttttctga taccgaaatc tttttatttt gctgacatac tcaaaccttt ggcacaaagt
     9841 ttcgtgccaa agtacgacta tttctctttt tagtaagcgg atacaattag agtatcaaaa
     9901 ggagagaggg agagacaaaa tggaacggac gattcccaaa aacaacattt cgctaaaagc
     9961 gagggtacaa aaattaggca gtactttatc aagcatggtc atgccaaata ttggagcact
    10021 gattgcctgg ggtgtattaa cggctctatt tatcccagat ggttatttac caaatgaggc
    10081 tttcgctacc atggtaagcc caatgctaac ttatctgatt cctttattga tcggttatac
    10141 tggcggaaaa gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg caacgatggg
    10201 tgtgattgtc ggaacagata ttccaatgat gctaggcgcg atgatcatgg gacctcttgg
    10261 tggatatgcc atcaagaaat ttgatcagct attccaaaaa cggatcaaat ccggttttga
    10321 aatgctagtc aataattttt cagcaggtct tatcggtttt ggattagcgt tattaggttt
    10381 ttctgcgatt ggtccagttg tcgatgcatt gactcaagca atggcaaaag gtgtagagat
    10441 cattttatct gcccatttga ttccattgac aagtattttt attgaaccag caaaaatctt
    10501 atttttgaat aatgcaatca atcatgggat tttgacacca ttgggaactg aacaagtagc
    10561 agaggctgga aaatctattc tattcttgct tgaagcaaat cctggtccag gacttggcgt
    10621 attacttgct tttactttgt ttggcaaagg agcagcaaaa tcttcagcac caggagccat
    10681 gatcattcac tttttaggcg ggatccatga aatctatttt ccttacgtta tgatgaaacc
    10741 gttattattc ttatcagtta taggcggagg aatgtcagga agtttcattt tccaattgat
    10801 ggatgctgga ctaagagcgc cagcttctcc agggtcgatc attgcgattc tagcgatgac
    10861 accgttcaat tcttaccttc ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt
    10921 tggtatttct gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa
    10981 atctgttcaa gagacaaagg cagctaagca agcttccaaa ggtgcccaag gtattcctca
    11041 aggagctaca ttctctggcg tacaaaaaat catttttgct tgtgatgcag gaatgggatc
    11101 aagtgcgatg ggcgcttcga tcttgagaaa aaaagtacag gaagcagact tgcctcaaac
    11161 ggtgaccaat caagcaatca gccaactgac agatgactcg aatacattga tcgttactca
    11221 ggtcgaacta caagaaagag caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga
    11281 aaactttttg aattcacctc gatatgatga aatcattcaa gaattaagca gcaagcagaa
    11341 aacatcagtt tctgaaattg agaatgagcc agcagaagag tttctttcag caaacatcaa
    11401 acaaatcaaa gagattctac ttgtccacga tgatcgttcc ggatcagcaa caatggggat
    11461 gaccgtgctc aaagatatat tagacaagaa ccatttaact atcccgttaa gaaaagtcaa
    11521 tatccaagat ttaaaagatg aacctgattc actgatcgta gccaaagaaa gtttagcgga
    11581 aaaagtacag atggctgcac ctcaatccat tcatttatca gtcaacagca tggtcaatcc
    11641 gcaaaaatac gaatcgattg cacaatattt gaaacagaca gcttaaggga ggaaaacagc
    11701 atgcaaagtt tgccattcga catgatcgag ctcaatcgat cttttaaaac caaagaagaa
    11761 gcgatccgct attgcggtca gatgcttgta gaagcagggt gcgttgatga ggaatatatc
    11821 gatgccatgg tagatagaga caaaatgtta tctgtttata tggggaattt tatcgcgatt
    11881 cctcatggaa cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa
    11941 gtaccagatg gtgtagattt tggtaccgaa gaagaagaaa agattgcgac tgttttgttc
    12001 ggtattgcag gagtcggaga tgagcatttg caacttgtac agcaaatcgc actttattgc
    12061 agtgatatgg acaatgttgt ccagctagca gacgcgctca gcaaagaaga agtaacggaa
    12121 aatctagcaa ttgcttaagg agagagaaaa atgagagcag tacattttgg tgcaggaaat
    12181 atcggtcgag gatttattgg agaggttttg gcagctaatg gatttgagat tactttcgta
    12241 gatgtgaatc acacgatcat tgatgcttta aatgaaagaa aagaatacga aatcgaatta
    12301 gcagatgaat caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa caatcaaaca
    12361 aatccagaaa aagtaatcca agaaatcgct gaagcagatt tagttactac cgccattggc
    12421 ccgaatatct taccttttat tgctgagttg attgcaaaag gtatccaaga aagagaaaag
    12481 gaaggaagca caacatcttt agatatcatt gcctgtgaaa atatgattgg cggttcagca
    12541 tttctagaaa aagaagtgta taaatatctt ccggaaacat catttactga caaatatatc
    12601 ggctttccaa atgcagcagt cgatcgaatc gtcccattac aacaccatga agatccttta
    12661 ttcgtccaag tggaaccttt caaagaatgg gtcattgacg atagccaaag aaaaaataaa
    12721 aaaatccaat taaaaggggt tttatatgta gatgatctag aaccatatat cgaacgcaaa
    12781 ctatttagtg tcaatactgg tcatgcgact gtcgcttata caggtgcctt gttaggttac
    12841 cagaccattg atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa atcagttttg
    12901 caggaaacag gaagtttgct gattgccaaa tgggggtttg atgcagagca gcatcatgcg
    12961 tatattgaaa aaatcattca tcgcttccaa aacaaaaata tctctgatgc gatcacacgt
    13021 gtcgctcgga caccattacg taagttaggt tatcaagaaa gatttacacg tccagtgaga
    13081 gaactacaag aacataactt gacttgccca catttgacgg caacaatggg gattattttt
    13141 aattactatg atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa
    13201 aacttggaac aattgattca agaagtcaca ggaatcaatg atccgaaaac aatcgggaat
    13261 atcaagcaaa acgtgaaccg ttatgccaag caagtcgctt aaaataggat acgaaaaagg
    13321 ggaatgtgac gaaatttttg tcacattccc ctttttcgaa taactaggct gtttgtcctt
    13381 caagataggg cagatccaat tctttaccgt aacgttgttc taaagctagg cggatcaaac
    13441 gagctgccag gttctcattt cttgcaagga taggtccatg gaaataagaa ccgaagacat
    13501 ttcggtaaat gacaccttca gattggtctt caccattatt tccttgacct tgtaccactt
    13561 tccccaaagg tcgttcacct tcacctaaga aagtccgacc attgtggttt tcaaatccat
    13621 aataagtttc attgaattct tcgttatgga taacgatatc accgataaac cggttatttt
    13681 cctgacttaa cgtgtagtga tccagtgcac tgattccttg gatcttttct ccattagctc
    13741 ccatgtagta gtgacctagc aattggtagc caccacaaat ggctaacatg acgccatcat
    13801 tttcgatata gttgatcaaa gaatctttct ttttctggat gtcttttgaa atgattaatt
    13861 gctcaaagtc ttgaccaccg ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt
    13921 catgtatact aacgatctcc gtatggcaag aaacccccat tttttctgct atatatttca
    13981 acatcaaaag gtttccgtta tcaccatatg tgttcatcaa atttccataa aggtgggcga
    14041 tattgagttc ataattagcc acggttcatt cctcctttga taaatccttg agcagctagt
    14101 tctttacgaa gctgcaatac agcagtgtaa gtagctaaaa tatagacatg ctcagttggc
    14161 aattctttga ttttttcgat tacttctggt aattcttttg tttctgtcaa tttattttct
    14221 ggaatcccgg caacttttag acgtaaagtc atatctgtat gacggtctcc tccagaaata
    14281 acagctggga tatccatatc ggcaaagttt tcaaaattac catcccaaat ccagctgaca
    14341 tcgattccgt ctgcgtaatt ggcatttaat aaagccacta aagaaaatgg ataaggagct
    14401 agaccaatca tatcgattac ttgattgata cctacaggat ttttgacaag tactaatgta
    14461 cattttttcc cattgatatt gatgacttct tgtcggccga atactttctc gtcatatcct
    14521 aaaccttgtt tgattttatc aggatcgaca ttgaagtact ctgccactgc agtagctgct
    14581 aaagcattgt agacattata cataccgcca acttcgattc cgtattcatg accatcgatc
    14641 acgaaatctg ctgacacatt gtccatccgt accatatcag ttaatcgata atccaattca
    14701 ggacgtttga agtcacaatt tggacagtaa tatttcccta agtttgcata agtgatcatt
    14761 ttataatgca agatgtgatg acaatttgga cataagacac cgtctgtatt gtaatgagcc
    14821 atttgttcac gatcttcttc atggtcaaag ccataatatt ttctaggatt gactgtttcg
    14881 atagaattga agattggtga atcaccgtta ctgataatcg ttgcatttgg tgactttgcg
    14941 gcaccgtcta caatcatttt ataggtcgta tagatttctc cataccgatc catttgatca
    15001 cggaaaatat ttgtaaataa gaacagttct ggtttcatat attctgtgac tttgctcaaa
    15061 cttgcttcat ctatttctag gacagcaaac tttttctgtc ctttttttgg ttttgcctgc
    15121 aagaaagtcg atacaattcc ctgtaccata ttcgcaccag tagggttggt tagaacttca
    15181 tcgaattgct ggcgcaaaat attgactgtt aaagcggtag tcaatgtttt accgtttgtt
    15241 ccggtgacaa cgacgacttg gtagtctttt gccaaactat ctagtatatg tggatcgact
    15301 ttcagcgcta gtttacctgg taaacttgat ccgcctttaa aaaaagtttt taatgcccat
    15361 tgcgacgttt ttccaactgc aatcgcaaaa tgactgcgta ttcccattat gtaacctcca
    15421 aaaacgattt gctgacgaat aaattcaact aatcatacca tatgttgaaa aaaaggttaa
    15481 attgcttttc ttattcttaa gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat
    15541 cgcatgattt aacaaaaaaa cagcttgaaa caaaatgatt gtttcaagct gctgtgattt
    15601 tattattccc tcttacctat attcccattt taaccgataa taattttctc gcttggatat
    15661 tcatagccta gatctcgtgt ttcttttggc acgaataaca tcaatgtata tagcattccg
    15721 atccgcccaa taaacatcag tgtagcaatc gtgattttgc ccacaacaga caagtctccg
    15781 gtaatcccta aagataaacc agttgttcca aacgcagaag ttacttcgat gatgatcgag
    15841 atcagcgttt gttcttctgt agcagataaa aaaacgatac aaaagaaaca cataccaaga
    15901 gaaagcataa agacaacgac tgattttcgc acatcatctt gatcgattct ccgtccaaaa
    15961 atattgatgt tatcttcact tttgaggaag gaatatagat acaaaccgat gattgctaca
    16021 gtagttgttc gaatcccgcc gcctacagaa ctaggactgc atccgataaa catcagtaaa
    16081 gaaaaaatga tcagtgttgt gatttgaaaa tcacccaaat cgtgaatctg cagaccagca
    16141 ttacgagtcg taatcgaata aaacatcgaa ttgatccatt tgacactcat attggaatct
    16201 tggaataaat ggtccttttc caatagataa atcaagacag ttcccgaaac aaacaagata
    16261 acaaaagcta acacagctaa tttggtaaac aaggaaaaac gaaacggaag cttcgcattg
    16321 gaacgtttgt ataatagcca ttcccggcac tccatcaata caggaaaacc aattcctccg
    16381 acaaaaatca aaaacatgat caggatcaag aaaaaatagt catgtgcaaa gggcttgatg
    16441 gaatccccag tgacgtcaaa cccagagttc gtgacagccg atatcgcctg ataaaaaccg
    16501 taaaaaacgg catcacgcca acgatcaaaa tagcctctgt aataaaaata aatagaaaag
    16561 aatgtcccaa aaagtaattg aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg
    16621 acgataccag acaaacgcgg ttgattcata tccgtcatga tcagctgtcg ctgtttcagt
    16681 gtgatccgtc gtttagaaaa aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca
    16741 ccaacttgaa ataatatttc cagcagtata ataccgttat cattgaacac agaattgata
    16801 tcaaaagtcg atagtccagt cacactcact gtactgattg ccatgaacag catgtccaca
    16861 aaagaaacat ggctatctgg ttcgcgaaaa atcggcatat aaaatagaac gagtgataag
    16921 accgtcatca atatataata aaaaacgatg atttggatcg atgagaaatg attggcagca
    16981 aacttctgtc ctcgccgctg cagccttcga aagaataaat caacatgcac agtactatcc
    17041 tcctgacaat agtcgtttct accagtatac aaggggaatt aggaatactc aaagaaatta
    17101 tcaaacagat aacaatacag ctttcgatta aaatagaaat ggcttataat aaggaaaatc
    17161 ctagaaaaaa gaaggaggag gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa
    17221 atgaaaaatc aaaaaataaa ttatgataca gttctgaaaa aaatgatcat gcaatgggag
    17281 aaagaagaag taaagcctaa gatattaata catacatgct gtgctccttg cagtacgcac
    17341 acattggaat tcatgtctca atatgcagag atcacgttat ttttttcaaa ttcgaatatt
    17401 catcctaaaa gtgagtattt gcgaagatta catgaacaaa aacgatttgt agaccaattt
    17461 aatgagaaaa ctgaccatca tgttgcgcta atcgttgatg aataccgtcc aaatgaattc
    17521 accaaaatgg ttttaacaaa ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt
    17581 gcatgcttca atatgcgatt agatcttgtt gcgcaaaaag cgcaagaact aggatacgat
    17641 tactttggaa gtgccctgac catctcgcca aagaaaaatg cagctttgat caatcagatc
    17701 ggaatggata tccaaaagat ttacgggaca caatacttgc caagtgattt caagaaaaac
    17761 aaaggctatg aacgctcttt gcaaatgtgc aaagagtatg atatttatcg ccaatgttat
    17821 tgtggttgcg tgttcgctgc caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa
    17881 gcaaaagctt acttaaaaac atcaaccgta gtttttgagg aaaattaaag aaatctttcg
    17941 gaaaaacgta agaaaaaaat aaagaattta tggtagaaat aaggtagact gtataatttg
    18001 tccgtagtaa agtaaggaaa caagaataaa tttcaatgaa ggaagagata agatgcgaaa
    18061 agtagcacct attttgaagc caatcgctgt attaacaatc tcgacgattg tcgcgactac
    18121 tacaggtatt gtgaatagtt ccagctaccg tgcaccaagt tcaagttcta gtactgtaga
    18181 agaaacaaca ccaactactt cagtctcgac atctgaacca acaacaacgg aaagtttgaa
    18241 tgaaaccact acgacaagta catgggacga tacaacaact agttcgtcgt atgatgagac
    18301 atatacaact gacagctcaa gcgaaacaag taatacggta ccagatggca ctccatcgga
    18361 ttcaaccaat ccaagtacaa gtccggatac aagtgctagc tccgaaacga taccaagcag
    18421 tacgactgta gatgataaca cgcaaaatga tgatgcagca cgtacagcac agcaaaacca
    18481 agattcagga aatacccaaa cgtataacca gcaagcaggt gattctcaat gacattttta
    18541 ctagacctta tcaacggggt gcataaattc cttggctatt tagacatcag tcctaaatat
    18601 cttaaccgtg gatatacgat tttaagtgtg attcccacgc tttatttatt gagaattgtt
    18661 tatggacttt ggcaaaacca aaactatctt caatttttct tatatggact tgcatttcta
    18721 gtgcttgttt attttactgt tttaaatgta ttttattact ttttagataa aaacacgaaa
    18781 gccgatgtca ctcagctttt tgtgaagtat ttacctgatg aagcgttcaa catccagaca
    18841 gaagaatcga tagataatgg aaattcgatc gataaagtga atacacaaga ggtcatggtt
    18901 tcttatgacg aagattatca attgaaatta gccgagaata tgcggtattt gattggaaat
    18961 ggtgagatca aaacaaatga actcggtcgc atggatggat ttctggtcga tagaaataca
    19021 ttgtatcctt attactatgt caagaaaaca ggagagaaag agtatgttct aaatattgga
    19081 cgaagctact cggacatgga aaaagtaggg accattcgac ttgactcaac agaagacaca
    19141 ttggaacctg tcggattgtt catcgtcggc ggtgattttg tcaaagacgg gttacgctat
    19201 catgagcctt atcgtctgaa attgattgca aaaaaggcac agtcgcagga agaaccagtg
    19261 gtttatagtc gtagccaaag aagaagacat tcataatgtg atgaaacgcc gtgtatcagt
    19321 ggttgcgata aaaacgttta ttcggaataa gggggatgtg acacgtcttt tgtcacgttc
    19381 cttttttata taacgagaaa gataagacga acacatcact tattctttcc tttcatttat
    19441 aaacattgta gaataaagaa agtacaacga agtattgtga aaaaacgtaa taaataacga
    19501 ggaaaataca agtaggaggt aagctgtgaa agaatttttg aaaaattggg ggattttgat
    19561 ccttgtatta gcggctattt tattagctag agtgtacgtg tttacaccag ttactgtaaa
    19621 cgggcattcg atggacccta cattaagtga tggacaacga ttgatttcat ctaagatctc
    19681 aaactatgaa cgaatggata ttattactac aaaggaaccg ggggatgaag aacggatgat
    19741 tgttaaacgg atcatcggta tgcctggaga tacagtcaag atggaaaatg accagttgac
    19801 cattaatggc aaaaagtatg atgaacctta tctggatgag ttcaaaaaag aattttcaga
    19861 agataagtta caaggagaat atgcttatag ctcaggattc caagcacaag cagaaagttc
    19921 atccaccttt acgagtgact ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg
    19981 cgataaccgc ttgatttcaa aagacagccg aatgtttggg ttagtcgaca aagatatgat
    20041 ccaaggaaaa gttgttttcc gttattggcc tctttcagaa attaaaatca tgtgatgaaa
    20101 aaataaaaag aagctgtgac atgttttttg tcacagcttc tttttccgaa aattccttct
    20161 tatttcttgg agttgctcac tttttttgca atctcatttt aagctttttg gctgatggat
    20221 aagaaaagcg aagcgttttt gttcataaat agcttttccc cacgatggaa ggtatgacta
    20281 gtagcagaga caaagcccgt tttttccaat cgattattca attcttcttg gctaaagcca
    20341 ttatggactt tcggatgact aatttgttca ttcttgtcaa agtctacgat gatcaattga
    20401 ccgccaggag ctaaaatttc ataaagtttt gtaagaatat tctccgtatc aggaatgtgt
    20461 aaaagtacaa gtgatagaag gatgagatta gcttggacag caggaaattc gacagaagca
    20521 tcagcgtgga tcgttcggac atttttcaga tctgctgctt ggatcttttc ttctgccatt
    20581 ttcagcatag tttctgaagc atcggcaatg atcagttctt tgaatctttc cgcaagcggt
    20641 aaactaacaa gcccagttcc gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc
    20701 ggtagataag tagttagttc ttcgcgaata atcttggcta atgcttgacg gtcaggggaa
    20761 tcataatggt tagcgatgga atcaaaaata ttttttgtca tcaaatagct ccttacgtaa
    20821 aactttgaaa ttttaactta ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat
    20881 tcaaaaaggg agaatctaga aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa
    20941 aaggaaagtg tgtccgagaa atattactag acttgcctga gtggtttggg cttcctgaat
    21001 caacggaaaa atatatcgag gaatcaagta aactaccttt atggtgtgaa aaaagaaaag
    21061 aagaatattt aggctttatc actctttcac agacaagtga agatactgcc gaaatctatt
    21121 ctattgtatg ggaataaaaa aagactatca tcaccaagga ttaggaacaa aactttttaa
    21181 agaagcagaa ggttacgcag caaaacatta caaatatttg caagtgaaga ctgtagacga
    21241 ggggcactac tctatatatg atcaaactat ttgtttctac gaatcattag gattttcaag
    21301 gttggaagtt tttccaaatt tatgggatga atggaaccca tgtttagttc ttgtcaaaaa
    21361 attggaacaa aagtgagcaa tagatttaat actaatctgt gcttgatgaa aattctcact
    21421 gtaaaaaatg ctgaaaatca agtataatac cataggaaca aactggaagg tgaaaaagag
    21481 catggcacaa ctatttttta agtatggcgc aatgaatagc ggcaagacga tcgagatcct
    21541 aaaagtcgca cataattatg aagaacaaaa taagccagtt gtcttaatga caagcggtat
    21601 tgatacaaga gatggtttag gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat
    21661 gccgatattt gaagaaacag acgtatttga cgtaattcag aaaatggaaa gcaaacccta
    21721 ttgtgtgctg atcgatgaag cgcaattctt aaaaaagaaa catgtcattg cttttacgag
    21781 aattgtggac gaattggata ttccggttat ggcatttggt ttgaaaaatg attttcgaaa
    21841 cgagttattt gaaggatcaa aatatttatt gctttatgca gacaaaatcg aagaaatgaa
    21901 aacaatctgt tggttctgcc acaaaaaagc aatcatgaat ttacattata ttgatggaaa
    21961 ccctgtttat gaaggcgatc aagtgcaaat tggcggaaat gaagcttatt accccgtttg
    22021 tcgaaaacat tactttcacc catcaatgat tacagaagga gaataagcat gtacgatcaa
    22081 ttacaagcaa ttgaagaccg ttatgaagag cttggagaac ttttaagtga cccagaagtg
    22141 atttctgata caaagcgttt tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca
    22201 gtcgaagttt atcgtcgtta taaagaagta gtacaaggaa tcaccgatac agaagaactg
    22261 cttggtgaaa aactggatga tgaaatggct gaactagcaa aagaagaatt atctgaatta
    22321 aaaaaagaaa aagaagtctt agaagaaaaa atcaagatct tgcttttacc aaaagatcca
    22381 aatgacgata aaaatatcat catggaaatc cgtggagcag caggcggaga cgaagcagca
    22441 ttattcgctg gcgatctttt caatatgtac caaaagtatg ctgaagcaca aggctggaaa
    22501 acagaagtga tggaagctaa cgttaccgga atcggtggat ataaagaagt gatcatgatg
    22561 atcaccggag acaacgtata ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag
    22621 cgtgttcctt caactgaatc gcaagggcgt atccatactt ccaccgcaac agttgtcgtg
    22681 atgccagaag ctgaagaagt agagatcgat attgaagaca aagatatccg tacagatatc
    22741 tatcatgctt caggtgctgg cggacagcac gtcaataaaa cagcatctgc tgtacgtttg
    22801 actcacttac caacagggat cgttgttgct atgcaagatg aacgttctca aatcaagaac
    22861 cgtgaaaaag caatgaaaat cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca
    22921 caaagcgagt atgatgccaa tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg
    22981 atccggacgt ataatttccc gcaaaatcgt gtgactgatc atcgtatcgg attaactatc
    23041 caaaaattag atcagatttt agcagggaaa ttagatgaga tcgttgatgc attgatcatg
    23101 tatgatcaaa catcaaaact ggaagctatg caaaatgggt aaaatgacct atcgagaagt
    23161 cctgtcacgg gcttcttctt ttttagagga tcatgggaaa gaagcatata gtatccaatt
    23221 tttatttttg gaacgaaaac aatggaaaaa acttgattgg ctccttcata tgaacgaaga
    23281 gatttcagaa gaagaacagc ggttgatcga aaccgatctg cgacttctgc ttgcagatca
    23341 tccacctcag tatttgctag gatatgcaga tttttatgat catcggttaa aggtaactga
    23401 agcaacactg atccctcgac cagaaacaga ggagttagtc gaatggtgtt tagacgagac
    23461 gcctggtgta ccgttagaag tgatagatat tgggacaggg actggagcaa ttgctattag
    23521 tttgaaagca gcaagaaaaa attggcatat atctgctgtg gatctttcag aagaagcatt
    23581 agaagtagcg aaagaaaatg ctcagaaaga aggaacgaaa atcagttttt atcatggaga
    23641 tacattagga ccagtaatgg atcagcaatt tgatgtgatt atttctaatc cgccgtacat
    23701 cagtcggaat gaatgggaac tgatggatga aagtgtccgg tcattcgaac ctaaaatggc
    23761 cctttttgct gagaacgatg gattagctat ttatgaaaaa atagcagttg aagcaccaag
    23821 cgtactaaaa tcaaatggaa agattttttt agaaatcgga tttcgccaag gtgaagcagt
    23881 aaaaaaaata tttcagcaag catttccaga taaaaaagtg gcgataaaaa aggatctttt
    23941 tggcaatgaa cgaatgatcc aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt
    24001 gtttttttat tataaaaaaa cacttatttg acaaggatgt aaacgacttt ttggtttatc
    24061 aacaagttta tccactattg acaaatagat tatccacaaa cagtggaaaa cttgaggata
    24121 aactcagaca aaataagaat gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac
    24181 gatatgatga aacagaattg caagaggcag ccgaagcact gaaagaagga gaacttgttg
    24241 cttttcctac cgaaacagta tacggattgg gcgccaacgc tcttttacca aacgctgtca
    24301 agaaagtgtt ttctgtaaaa gggagaccgc aagataatcc attaatcgtc catgtcgcct
    24361 cttttgaaca agtaaaagag tatgtggaca actttcatcc ggcagcagag aagattgtga
    24421 agaacttctg gcctggacca ttaactttga tatttaagat caaaaaagac actttgccct
    24481 cagttgtgac gggaggatta tccacagctg catttcgtat accggataac aaaaaaacac
    24541 tagaagtaat cgaactttcc ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa
    24601 aaccaagtcc aacgactgca gatcatgtat atcatgatct acaaggaaaa attaccggga
    24661 ttattgatga tggagctacg cgaattggtg tagaatccac tgttcttgat ttaagtgatc
    24721 caacagccat gcctatgatt ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag
    24781 tcatcgaatc tcctgttgct attgatcagc atcttgtaaa agaaaatgaa acaccaaaag
    24841 cccctgggat gaaatacaaa cactactcac ccgatacacg tgttctgatg gtcagagatg
    24901 gcgattggtc aactgcagtt caatgggcaa aaaataagaa gatccgtgca ggtgtcatag
    24961 caagccccga aattgcggat caagtgcgga cagatactgc agctgtttat atgtataacg
    25021 acaactcagt agaagcggcg gctaaaggat tgtttgcagg tctaagagga ttagatgaac
    25081 caacacttgg attagatttg atttttgtac aagtgtatcc agaaactgga ttggggaatg
    25141 cttacatgaa tcgcttgaaa aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg
    25201 gttattgtcg cattctttta gtatgataca atgctatcaa gtaaatcttc tatcagtgaa
    25261 cgaaaaacta agcgtaacta atcgtaatag aagagatacg aatatttggc aatttgtttt
    25321 tgatgggaaa aataaaattg taggaaaata aaaaggagct gttgtttgtg gtagattaca
    25381 aaacgtttga ccctgatctt tgggctgcga tcgccaaaga agaagaaaga caagaacata
    25441 atttggagct gattgcctct gaaaactttg tctccgaagc agtgatggct gcacaaggaa
    25501 gtatcttgac aaataaatac gcagaaggtt atccagggca tcgctattat ggtgggtgtg
    25561 aatttgtcga tatcgttgaa aatttagcga ttgaccgtgc aaaagaatta ttcggagcga
    25621 aatttgcgaa cgtccagcct cactccggct ctcaagccaa cacggctgct tatctcgctt
    25681 tggtagaacc aggggacacg atcttaggaa tggatctttc cgcaggcgga catctaacac
    25741 atggatcacc agtcaacttc agtggcaaaa cctatcactt tgttgcttac ggtgttgatc
    25801 cgacaacaga agttattgat tataatgtgg tacgtatttt agctagaaaa catcaaccaa
    25861 aattgatcgt agcaggtgcc agtgcttacg gacgaacgat cgactttgcg aaattcagag
    25921 aaatcgcaga tgaagtaggc gcaaaactga tggtagatat ggcacatatc gcaggattag
    25981 tagcagcagg acttcatcct aatccagttc cttatgcaga tatcacaacg actaccactc
    26041 ataaaacatt gcgtggccct cgaggcggca tgattttgac aaacgatgaa gcattagcaa
    26101 aaaaaataaa tagtgcggta tttcctggta ttcaaggcgg ccctctagag catgtgattg
    26161 cgggtaaagc agtagcattc aaagaagcat tagatcctgc atttaaagaa tatagtgaac
    26221 aaatcatcgc caatgcaaaa gcaatggtca aagttttcaa tcaagcaatt ggaaccagag
    26281 tcatttcagg ggcgacagat aatcatctga tgttgatcga tgttcgcgag ttaggtataa
    26341 atggaaaaga agcagagagc attttggaca gtgtgaatat cactgtaaat aaaaactcga
    26401 ttccttttga aacactaagt ccattcaaaa caagcggtat tcgaatcggc acgccagcaa
    26461 tcactactcg cgggttcaaa gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag
    26521 cattacaagc aaaagatgat aacgctcaac tagacgaagt caaaacaggt gtacgcgaat
    26581 taactgaaaa atttccgttg cataaaaaat aaaaatttaa attccattag taaacagaaa
    26641 agttcaatgt tgggcttttc tgtttatttt ttgggaaaac ggattctttg acaatatcgg
    26701 tgatgctctc tagtatatga ggtgtaattt tagtcttcac actaaatctc agggaggaaa
    26761 agtcatgact gatcgtatct cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc
    26821 gcctcatccg cgtatctatg aactagcacc ggaagaagga agagttttac tagaagaagt
    26881 acaagaatca cctgtgaaca aatattccgt cgatattgaa gacttgatgg tggatactgg
    26941 cgaatgggga aaactcaata ttcgtctcgt acgaccagaa gggaatatgg ataaattacc
    27001 agttattttt tatattcatg gagcaggctg ggtttttggc agtgctcaaa cccatgataa
    27061 attgatcaga gaacttgcgg ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg
    27121 atcaccagaa gcaaaatatc caacagcaat tgaacaaaat tatgctgtgt tgcaacagct
    27181 gaataaatta gccaagtcaa aaaatctgga tctcgagaat ctaatcgttg caggagattc
    27241 agtaggtggt aacatggcta cagtcatgac gatcttaaca aaagaacgaa agggcctccc
    27301 aatatccaag caattgctgt attatccagt cacaaacgct gattttgata cagattccta
    27361 tcatgaattc ggagaaaact attttttgac aagagaagga atgatgtggt tttgggacca
    27421 atatacgacg aatgaagaag agcgtgctga aatcacagct tcgcctttga gagcaaatat
    27481 aaatgaacta aatgatcttc cttcagcaat gattttaacg ggagaagcgg atgtattaag
    27541 agatgaaggt gaggcttatg cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt
    27601 acgcttccaa ggaatcatcc atgattttgt catggtaaat gctatggatc agacaaaggc
    27661 aacgcgtgca gcaatggcat tatctacttc ttggatcaag aaaaaagaat aagctttgct
    27721 tgtccgcagg aaaacttctt ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac
    27781 tagtcaatca cttgattttg ttttacaatt aaaaagaaac gacagaacaa aggagagctt
    27841 aaaaaatggg caaatttcaa gtgatcgatc atccattgat tcaacacaaa ctaactatta
    27901 ttcgggacaa aaactgcgga acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt
    27961 tgatggcata tgaagtatca cgggatatgc cattggaaga catcgtgatc gaaacaccga
    28021 tcactgaaac aacacaaaaa acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc
    28081 gcgcaggtat tggaatggtt gacggtatct tggaattgat tccagctgct aaagtgggac
    28141 atatcggtct ttatcgagat gaagaaacat tggaaccaca tgaatatttc gtcaaacttc
    28201 cagaagacat tgatgcacgt cagttgtttg tagtagatcc aatgttggca acaggcggat
    28261 cagcaatcat ggcaatcgat gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt
    28321 gtttggtagc agcacctgag ggagtaaaag ctttgcaaga agcgcatcct gatattgaca
    28381 tctatacagc ttctctagat gaaaaattga atgaacatgg ttatatcgtt cctggactag
    28441 gggatgcggg tgaccgtttg tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac
    28501 aacttttgtc acatcctttt ttggtcattt ttaacgtttg atgcagaaca gctttttcac
    28561 agctttcgtt tgcatataaa aaaaccatcc caaaaatgca gagatggtca aattcaaatc
    28621 ttttttagtt ctttgtagtt aacgattact acacccttac tttgcgtcat caagttcgca
    28681 ttttctgcag tttcatcaat ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt
    28741 ccagtttgcg acttgccgtg aattgtgaac attacgtgag tattgatctt gaatttttca
    28801 ccaatttccg atggtacgat agttgtatca aattttccaa aagttgccat tttcataaaa
    28861 cctgcctttc acatcaatta taccacgttt tatgaaaaat atcatgaaat taaactaatt
    28921 ttccatcgga ctctttacaa tcagccaaat agagtatata atttcttttg gataaggcca
    28981 gcttagctca gttggtagag caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta
    29041 tagctggcat taaaagagaa gacagatata tcaaggattt ttaatccatt gatacatctg
    29101 tctttttgtt taaaggaaat aaagtatgct ttcttagcaa tagagcatgt attatttcta
    29161 taaaaattta ggagaataaa acgactagca atatagctgt gaaatcaaat atcacatgaa
    29221 ctgcccaact tgtaatgatc gagttgcttt taatggcagc ttggttgaat aacaatcttg
    29281 ccaccccttg tatcaaaagg atgtgtgcta cagtgtgtaa aatatttcca ttatcataag
    29341 tactgtaatg gataaatcca aagatgattg ctgacaagaa actagcgaca ggaatgctga
    29401 acttactaga aaaaatagcg agaaaataca ctgagaacaa ttcttctcca agtatcatga
    29461 taggcaaaat aataaataat aacgggctga aagcctcatt tattggattg cctgttaaac
    29521 cccatttcaa aatttgttgc aaaaataccg agactgcggc actgattact acattgataa
    29581 aaaaatattt ggctatattt ttgacaggag cgacaggttt cgagaacatt tttttcgtat
    29641 cttttttacc aaaaacaagt gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc
    29701 taataatgga tggcgcaaat agcaacccta acaaaccgag tacaacgaca atagtgccca
    29761 atatcccttt tttaagttct attttttttc ctaaaatgtt attcatgata ataactccta
    29821 ctattcagtt tttgaagata gagtca
//
LOCUS       DAEGIQ010000027        28270 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000027 DAEGIQ010000000
VERSION     DAEGIQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.027"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            7..1347
                     /locus_tag="KMT45_001070"
     CDS             7..1347
                     /locus_tag="KMT45_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7004543.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1542..1985
                     /locus_tag="KMT45_001071"
     CDS             1542..1985
                     /locus_tag="KMT45_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBE7004544.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2247..3665)
                     /gene="nhaC"
                     /locus_tag="KMT45_001072"
     CDS             complement(2247..3665)
                     /gene="nhaC"
                     /locus_tag="KMT45_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBE7004545.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4069..5292)
                     /locus_tag="KMT45_001073"
     CDS             complement(4069..5292)
                     /locus_tag="KMT45_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBE7004546.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5324..6163)
                     /locus_tag="KMT45_001074"
     CDS             complement(5324..6163)
                     /locus_tag="KMT45_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBE7004547.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAVFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6180..6860)
                     /locus_tag="KMT45_001075"
     CDS             complement(6180..6860)
                     /locus_tag="KMT45_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004548.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(6862..7935)
                     /locus_tag="KMT45_001076"
     CDS             complement(6862..7935)
                     /locus_tag="KMT45_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7004549.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(7994..8232)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8275..8508)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8585..9256)
                     /locus_tag="KMT45_001077"
     CDS             complement(8585..9256)
                     /locus_tag="KMT45_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBE7004550.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KKWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9344..12022)
                     /gene="mgtA"
                     /locus_tag="KMT45_001078"
     CDS             complement(9344..12022)
                     /gene="mgtA"
                     /locus_tag="KMT45_001078"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBE7004551.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWFFITVVAYLALVTAVKKIYIRRFQELL"
     gene            12682..13119
                     /gene="ndk"
                     /locus_tag="KMT45_001079"
     CDS             12682..13119
                     /gene="ndk"
                     /locus_tag="KMT45_001079"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBE7004552.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13141..14007)
                     /locus_tag="KMT45_001080"
     CDS             complement(13141..14007)
                     /locus_tag="KMT45_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7004553.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(14241..16319)
                     /locus_tag="KMT45_001081"
     CDS             complement(14241..16319)
                     /locus_tag="KMT45_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBE7004554.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            16812..18029
                     /locus_tag="KMT45_001082"
     CDS             16812..18029
                     /locus_tag="KMT45_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBE7004555.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(18488..19111)
                     /locus_tag="KMT45_001083"
     CDS             complement(18488..19111)
                     /locus_tag="KMT45_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004556.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(19267..20664)
                     /locus_tag="KMT45_001084"
     CDS             complement(19267..20664)
                     /locus_tag="KMT45_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBE7004557.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(20686..21657)
                     /locus_tag="KMT45_001085"
     CDS             complement(20686..21657)
                     /locus_tag="KMT45_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7004558.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(21664..23964)
                     /locus_tag="KMT45_001086"
     CDS             complement(21664..23964)
                     /locus_tag="KMT45_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HBE7004559.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(23966..25291)
                     /locus_tag="KMT45_001087"
     CDS             complement(23966..25291)
                     /locus_tag="KMT45_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7004560.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            25522..25947
                     /locus_tag="KMT45_001088"
     CDS             25522..25947
                     /locus_tag="KMT45_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004561.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(26445..27146)
                     /locus_tag="KMT45_001089"
     CDS             complement(26445..27146)
                     /locus_tag="KMT45_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBE7004562.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(27162..27602)
                     /locus_tag="KMT45_001090"
     CDS             complement(27162..27602)
                     /locus_tag="KMT45_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HBE7004563.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(27602..28177)
                     /locus_tag="KMT45_001091"
     CDS             complement(27602..28177)
                     /locus_tag="KMT45_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBE7004564.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         8198 a         5972 c         4872 g         9228 t
ORIGIN      
        1 ttatttatgg aagataaaaa attgaaaaaa caacttagct cccgacatat caccatgcta
       61 gctttaggtg gagcgattgg tgcaggtcta tttaaaggaa gtggcgaagc gattggaatc
      121 gccggccctt ctgtattgat cgcttttttg attggcggca taattttata tatcgtgatg
      181 aaaggtctgg gaaaaattgt tttaagtggt ggtgatacgc accacggatt atctggattg
      241 attcgtccat atctaggtgc tcatgctgcc gattttaccg attgggtcta ctggtccatg
      301 tggattatca atatcattgc tgaagctgtt gcagctgctt cctttttaca gttgtggttc
      361 ccccacatcc cagcttgggt atttgttttt atgttggctg ttttaactac tatcatcaat
      421 ctttattcgg tacgattatt cgcagagaca gaatattggc tggcctttgc taaaatcagt
      481 gtcattatcc tattgatcat ttttgctgct tatcttgtcg gtcagcaaat gttgggtact
      541 ggtgttttcc ctacccttca acaattaact gatcatggtg gttttactcc ccacggtatg
      601 aagggaatca tcagttcttt gcttgtagtt atctattctt acggcggttc tgagttgatt
      661 gctatcacag taagcgaagc agatgatcct aaacgtgcga ttcctaaagc aatcaaaggc
      721 gtcatgggaa gaatcatttc cttttacatt attcctttat ttttgctttt gattattttt
      781 ccttggaata ctttagcagg cacgaacgtt agtccttttg tgatggtatt tgaaaaaatg
      841 aatattccat ttgcggcaga tatcgtcaat ttcgtgattg ttcttgctct gttctcgtct
      901 atcaattcag gtgtatatgc ttcttcgaga attttgttct tccgcttgaa agatcggaaa
      961 ggttcctcta gaaaactagc tgttttaaac aagcatcaag taccgcaacg tgctgtgctg
     1021 ttttgtacca gtgttcttta tctaggtgtg gctctttctt attttgtcgg tgacaaatta
     1081 tttggctatc tggcaggttc tctttcctat acagtattat tgatctggat cattatcagt
     1141 gctgcgtcct ttgtactgac actaaggaaa ggaacgttat gggacaaagg aatcagcttt
     1201 tttgcattag cagtcttatg gttgattttc cttggtatcc tattcacgaa ctccattggg
     1261 gtaactgtat tgaccggttt gctttacctg ttcatttatt tcagttatca aaaaaagaat
     1321 gatgctttcg tcttgacgaa tgaatgaaac caatagagaa ttttattcta tttatattga
     1381 atatctggag ctattgaaaa aaaggagctg cgacaaaaat tgttgcagtt cttttttccg
     1441 aataaacggt gttcaaaaac catctgtgca gattaattga ttgttctcca cgctcatgac
     1501 atgatagaag aaaggaatga agaaaaagga gtttgtttta aatgaaaaag tattggaagt
     1561 ttttgttgat gattggtgtt tcgacggtag tcatgtttgg tatgatgtat ttgaatgttt
     1621 atgcgcttga tcatatcttc ttcagtcgca cacgtgtatt catggcgttg atgatgggtg
     1681 cagtcatggc agtcatcatg ttgttgttca tgtggaaaat gtatgaaaac aaaaagataa
     1741 atgctatgat tttagccgct agtcttcttg tttttgccgg ttccttattt atggtacgaa
     1801 gccaaacaac tgtcaacgat acggcgtgga tgaaggcgat gatccctcat cactctattg
     1861 ctatcttgac aagtaaacga gctgatttaa aagatcctga agtaaaaaaa ttagcggaag
     1921 aaatcattca ggcacaagaa gaagaaatcg acaaaatgaa aaaaatgatc aaagaaatgg
     1981 aatgaccatt atccatccaa ataaaaaaag aggatgttcc agaagtgatc aacttcaaga
     2041 aataagaaga agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa gctgccctgc
     2101 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttaggct
     2161 tattaccgca aatgcaagct tttgaacacc gtttattcgt aaaagggagt gtgacaaaag
     2221 tcttgtcaca ctcccttttt acgtttttat tgttctaatt gcgctgtctt gcttactgct
     2281 tcttgcggtt tcacaaattt ctttgagatc ccccaacctg taaaggcact gaccatcgaa
     2341 aacacaggtg aaagcaaact gaaaaacacg aatggcaagt aagtgagtgt aggaatgcca
     2401 aatgtacttg ctacgaaact tccggcgatt ccccaaggca ctaagtagtt gattactgtt
     2461 ccaccatctt ctagtgttct ccctaataca gttggatcaa atcctgcttt ttcgtaagtt
     2521 tctttaaatg cattaccagg caggataaca gatagaaatt gttctccaat aaagatattt
     2581 actccgatgc ttgtcatcaa tgttgtaaaa atcaattttg gggtgttcgt catctttctt
     2641 gatactcgtt ccataacggt tgagatcaac ccaaattcga tcaacaaacc acctaatgac
     2701 aatgttagaa taatcaatga cacagttggc atcatgcttt cgattcctcc acggctaagt
     2761 agtgaatcga tttgttggtt tccagtattt gaaacaaatc ccgattcgat gacccctgcc
     2821 aacttcgtta aggaagtttg tgggttttga ataaagatca ttcctactgc tacagcaata
     2881 ttcaacaaaa tcgtcaaaat tgccggtacc tttttccatg cacacaagag cattaaaaat
     2941 agaggaatca gtgaccagat cgaaatagta aagtttgctt ctaaaatatc agtcacttca
     3001 cgaatagatg aaagacttgc ggctttactt gtgtgtccta aaatcatgaa taaaaagaaa
     3061 gaaacaacaa atgccggaac agttgaccac atcatatgct tgatgtgttt gaacaaatcg
     3121 gcatcaacaa tagctgctgc taaatttgtt gattcggata atggagacat cttgtctcca
     3181 aaaacggcac ctgaaacaat ggctccaaca acaagggctg gatttagtcc tagtgttgtc
     3241 ccaataccga aaaatgcaat cccaatcgtt gacataacag taaaggcact ccctacagcg
     3301 ctacctacga ttgcacaaac aacaaataca gaagggacga accactggac actaatcaat
     3361 ttaaatccaa tgaccatgag ggttggaata attcctgctt gtatccatac agcaatcaat
     3421 gaaccgacta aaataaaaat aaagattgga atgatccccg gtttgatgcc attgatgatc
     3481 ccttcgtgca gccaatctgt cgggtatttt tttatcactg cgtacaacat gaccattccg
     3541 atagctgcca aaatcgtgat attaggtgaa acctttaatc cgacaacgcc aatcgcaatg
     3601 cctaaaacaa tgaccatcaa tacaagtgtt gcttctaaaa aacttggtgt tccttttttt
     3661 tccatgataa tctctccttt tttagttgtt cttatgttct aaaaaagtta agatatcgga
     3721 ttactccgcc acaattcatc cgcgattcct cctttataaa aataaaaaga cctgctttca
     3781 actaaggacg aatgactccg cggtaccacc ttaattaaaa acagatctgt ttttcacttt
     3841 caaacgatta ttcaaatgta tggactttcc atgtaattcg tcaattaggc ttgatcggtt
     3901 cacagcgacc accgactttc tgaacaaaac acctaaccac tactgcgtga aaagttgact
     3961 tgtatgaagt ttaccttcca taagatcatt tgtcaatgaa aaagaaaata ttttttcatt
     4021 tttctgaaaa ttatttttat aactttcgcg cacaacttta tctttttact agaaagtagc
     4081 gccctcttgt ttttttcttg ctactatttc ttgatgttct tgtttgattt tttctaatag
     4141 cttgggttct ctcaataaat ctagagctgt attggcaagt aattccgctg caatcgcaat
     4201 agaagcaagt cctttttcac ttttagcagc agctttgaat tcttctgtat gtccggcaat
     4261 atatgtctca gagattgcta cagtaggctg tatcgttgga ataacttggc taacatttcc
     4321 tacatcagaa gaacctagac ttgttctttg ttccgtttct atttcttcat caggaacccc
     4381 tgcttctttt gcatgttgga agaataagtc atcgaaagaa ggcgttacga tgacatcatc
     4441 gactgcattt tggaacaatc caaactcaaa agtcgtaccc gtgcttaatg cagcacctcg
     4501 tacgatattt tccacttttt catatacttt gtctaatgtt tgtcgatttc ctgcacgtaa
     4561 atagaaacgt ccagcagcgt agtctggtat gacattggcc gccacgcctc catctgtaat
     4621 gattccatgg atattgacat cttttggcaa ttgcaagcgt agagcgttga ttccgttaaa
     4681 cacttggatc aaggcttcca aagcatttac tcctttttca ggtgctgctg ctgcatgaga
     4741 agcgacaccg taaaatttga tatcgactgg atcatttgct aaagattctg tcgttttccc
     4801 ataacgaaaa gctggatgga cacataatgc tgcatcgaca tcctcaaaaa aaccttcccg
     4861 aacgaagctt cccttagctg atccattttc gcctccttct tcccccggag taccatacac
     4921 acgaatttct ccgccaatct catcaatgaa ttgtttcact atgctggctg ctaatacgga
     4981 gtaagtacca aataaattat gtccgcatgc atgaccaata cctggtaaag catcaaattc
     5041 tgccaaaaag gcaattgtcg gacctggttt attggaaata taacgtgcat caaagcctgt
     5101 tcggtgccca gctacttctt tttctaccca gaacccttct ttttccaatt gtttgattaa
     5161 agctgctgag gaataaaatt cataattact tacttcagga tggtcgtgga tagctagtgc
     5221 aagtgcttga tattctggta aacggtattg aatcagatcc ttcaattgat ttgttgagag
     5281 tgtggttgtc ataaaaattt cctcctatgc tagtcgtttt ttcttatttt ctcccgaatg
     5341 tttcccacgc aggtacgctt gaaccttttg atgttttttc gattactttg gctgtttctt
     5401 ctgtttgata ggcatctact attttttgat acgttttatt gtcttcatct tcttttcttg
     5461 caacgatgat attgacataa ggacgtgcat gctcatcgac tggttctaag aagattgcat
     5521 cagaagcggg attaaataca gcatctacag ctacaccgct attgatgact gaaatatcta
     5581 catctgacaa agcacgtgct gtttgggaag catctaactc agaaatgtct agattttttt
     5641 tattctcggt aatatcagaa acggtaggcg cttggccttt tgccttgtcc actttgatca
     5701 atccagcagt ttgcaataac agaagagctc tgccgccatt cgtgacatca ttagggattg
     5761 caacttttgc tccatctttg atatcagcca catcttttaa cgtattggaa taaattccca
     5821 acggtgcatt aaccgtattc ccaatcgata ctaaatcagt tccgtgttct tcattgtaat
     5881 tgtctaaaaa gaattgatgt tggaaggaat tcaaatcgat cgatccatct gctaaagccg
     5941 catttggctg attgtaatct gtaaatttca catattccaa atggatgttt tcctttttca
     6001 aacggtcttt cacatcgtcc catacatctg tatcttcccc aatgatccct agttttacgg
     6061 tcgtttcttt tttttcagtt gtagctgctt ttccttggct gcatcctgcc aaccaaataa
     6121 ttgctacaaa tgtaatgact gcaaaacgaa taattttttt cattttttct cctccatttt
     6181 caatgtgtga cttttttaat cagttgatta ctgataaatt gactcaaaaa aactaagacc
     6241 agaatcaata atgtagcgac aatcgttaca tctgtttgga atcgattata tcctctcgat
     6301 atagcaagat tccctaatcc tccggcacct actgctccag ccatggtagt cagtccgatc
     6361 acattgatga tcgtcaacgc tgagacccga atgataccag gtaacccttc taacaaataa
     6421 acacgtaaaa tgatgccgat tgggctcgtg cccatcgctt ctgctgcttc gatcacccca
     6481 ggatcaactt ctagcagtgc attctcgatt tgacgggcaa aaaacgggac aacgccaaaa
     6541 atcaaaggga ctaaagctgc tttggcacca atcgtcgttc caactaagaa acgagtagtc
     6601 aaagctaaaa gagaaagcaa gataatgaaa gggatggaac gaatgatatt gatcaactta
     6661 tcaagtattt cgaacagcag cctgttagct agtaatccat ttggacgtgt cactactaac
     6721 gtcacaccta agatgattcc gatgatccca ccaatgaatg ccgtccaaaa agacatatat
     6781 agtgtttgct ttgtcgcttc gatgaattca tccggaatca aatagacatt cggcaaatat
     6841 gttttaaaaa attcagtcat tttagtttcc tccttcgatg actttgatac ggttatctaa
     6901 ttcatttttt tgtaaaatag ttacttttac accagattta atcaaataat ccaatgcttt
     6961 ttgccgctgt ttggtttctc cagctaaagt aacgatcaaa cttcctaagg gtgtttcttg
     7021 taatagctct acattaccgt ataagatatt cgctgttact tggtacttgc tgtaaagatg
     7081 agcgattaga ggttcgttcg tctggttgcc gatataactt aattccacca accatttatt
     7141 tgcaatctga tcggcaaaag cagcatgggc actgattgtt tccaatgctt ggtcaaggtg
     7201 tgtcgctgtc cgaataaaat ctttagtcaa ttcttcttca ggatgactga aaatctggat
     7261 gctgctacct ttttcaatga ttttccccgc ttccataacc gctaccttgt tgcagatttc
     7321 tttgacaact tgcatctcat gagtgatcag gacgattgtt agacctagct gacgatttag
     7381 tttttttaac agtgcaagga tctgaagggt cgttttcgga tcaagtgcac tagttgcttc
     7441 atcgcataac aacactttag gttcatttgc cagagctcgt gcgattgcca ctcgttgttt
     7501 ttgccccccc gacagttgtt taggatacgc agatgctttt tcttccagac cgacaagttc
     7561 cagtagttca ttcacttttt gacgacgttc ctgttttgat tttccagaat actttagaga
     7621 aaaatctacg ttgtcaaaaa tcgttcgact ttccattaga ttgaaatgct gaaagatcat
     7681 accgatggcc tttctttctt ttcgcaattc ttttggagag agctggctta aattcgtctg
     7741 attgacgatg acttctcctg ctgttggttt ctgcaacaaa ttcatcacgc ggactaacgt
     7801 actctttcct gcacctgagt aaccgacgat cccataaaca tccccttttt caatcgttaa
     7861 atctacagaa tctactgcct gtatctcttt ttctttttgt ttgaaacata cagaaatatc
     7921 tcgcaattca atcattaaca ttcctccttg tctataaaaa aagcatcctc aagaaaatga
     7981 aaatagtcat tttcttgagg acgccttttt gcgtgttacc acctcatgtt caagctgtgt
     8041 tcacacacaa ctcctcttca agtaccatca tactctagca agatatcggt cgcagccgtc
     8101 accgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc
     8161 ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt
     8221 caatgcaaat aatattcaaa aaaaaaatcc ttttactaac ataaactatg tcagtaaaag
     8281 gacgacgtat cgtggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc
     8341 aaaaagagag attggcgttc gttttaacgg tcgaacattt ccgaatttgc ctacttatcg
     8401 acaaattatt ctccaaggcc attttcagct tttcttactt gactcttcac accagccaga
     8461 gcctctctgc aaagtttcca agcttacttt ccttctcaac gaaatcaata ttttgttgtt
     8521 gggaataatc tacaggagga aaactgaaaa gtcaagcatg aaatccaaat tattttttct
     8581 atatttattt ttctgttttt ttatctaaca tttcaataac agttcgaaga tcttttcctt
     8641 ccgcaagaag gttcaatttt tccttctctt cgacgctata atttcgaaca aaactggaat
     8701 cgggaatcaa aacaacgcct agtcctgcac gtgaagcggc tgtcgctcct actaaagaat
     8761 cttccactgc aaaagctgaa agagcttcta atcctgttcg ttccaaagct gtcagataag
     8821 ggactggtga aggcttgttc tccgatactt catctccgaa cgtaaacgta tcaaagtaat
     8881 cggctaattg aaagtgatcc aagatatctg ttgctctttt cgtaacagtt gtcgtagcaa
     8941 tccctagttt catatgacgt tcttttaagt ctgtcaatat ttcttttgca tagggtttca
     9001 attggatagc tccacttgtc agctgttgat aaaaatattc ttcacgcttt gctcggactt
     9061 cctgcacttt ttcaggactg cctgctgcct gcactaaaat atcaaaactt tgtttccatt
     9121 tttttccgcc ccaagcgtca acgatttctt tcgggatatg aatatcaaat tgctggaatc
     9181 cccaaagcca accatcccga tacgttcttt ccgtatccac taacaaacca tctaaatcaa
     9241 atattgcacc tttcatctat acattaatcc ttcccaaaac aatacaccta gtttagcatg
     9301 actgatccaa aatgaacaga agcatagagc ccagccgcat cacttataag agttcttgga
     9361 atcgacggat atagattttc ttcacagctg tcacaagcgc cagataagcg acgactgtta
     9421 taaaaaacca taaccagtaa ttgccaggga gcggcatcaa gcctaactca gcaccaaatc
     9481 ctgtgaatgg aaggactgaa ccgacaatga ttcctaatgt agtgataacc agcatggcaa
     9541 atgttgcatt gctttggata aatggtattt tcggtgtacg caatgcatgg agtactaatg
     9601 tttgtgtcca cagggattcg acaaaccatc ctgcatggaa taatgcgata aaaatcactt
     9661 gttgtgagct atccaatgta tggaaacttc caccaactac agcaggacaa ataacaaaat
     9721 acatcaacaa gtaagtagtg atatcaaaaa tcgaacttgt tggaccaaaa taaaccatga
     9781 attttccaat actagatgca ttccatttct ttggctcatc caaatattct ttatccatcc
     9841 gatcccatgg taaggaaatg caagaaacgt catagatcag attcaaaaat agaagttgca
     9901 aaggcaacat tggtaaaaat ggtaagaacg tactggcaat cagtacggaa aacatattgc
     9961 caaaattaga acttgctgtt gccttgatat atttcatgat gtttccaaaa gtttctcttc
    10021 cagaaagaag gcctctttcc aaaatcgtca aatctttttc taaaaggatc acgtctgctg
    10081 attcttttgc gatatccaca gctgtatcca cggaaatacc gatatcagcc actttcattg
    10141 ctggagcatc attgatacca tctcctaaaa atccgacagt atgcccattt ttacgtaaag
    10201 ctgtcgtcaa tcgtgctttt tgctgaggat tcaattttac gaatacttcg tgttcttcag
    10261 ctacttgcgc taattcatga tcattcattt ccgagatttt ctcaccagta atcaattcat
    10321 tgatttctag gcctacttga cggcacacag accgtgtgac taattcattg tccccagtca
    10381 gaacttttac agctacacca tgttctttta aagctttcaa cgcttgtttt gttgtttctt
    10441 ttggtggatc aaggaaagca agataaccaa tcagaaccat gtctgactca tctttgactg
    10501 aaaattctcc aacaacgctt ggattggtct tttgtgcgac accaatcact cgtaagccgt
    10561 cttcattaag tgattgtact tttgcaagca ccgcttcttt tcgttctttt gttaatggtg
    10621 cgacagtacc accaagatca ataaatttag aaatgttcaa catttcttca atcgcgcctt
    10681 ttgtgatcat ctgtgttttg ccagccggat cagcgactac aacactcata cggcgacgtt
    10741 caaaatcaaa aggaatttca tctacttttt gataattgat tgtatctgta tctaaagttt
    10801 ctgctgcttc atcaatgata gccacatcta acaaattttt cagccctgtt tgatagtagc
    10861 tgtttagata agcatggcgc aaaacacgat catcttcatt tccttcacaa tccatatgat
    10921 attcaagaat gattttgtct tgtgtcaatg ttcccgtttt atccgtacat aacacatcga
    10981 tcgcaccaaa attctggata gcatttaaat ttttgatgac tgttcctttt ttggccatca
    11041 cacttgctcc tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca ggcgttaaac
    11101 caactgccac agataatcca aaaagaaaag cttctaacca atctcctttt gtaagcccat
    11161 tgataacgat aactgctgga gccatcaatg acataaattt gatcagcagc atggatgttt
    11221 tgtggatgcc taattcaaaa cttgattgga tgggtttttc agatagcgtt tctgctacat
    11281 gcccaaatag tgtgtcattt ccgacagaaa caatgattcc ttccgcgctt ccactgatga
    11341 cattgcttcc cataaagaca agattttcat aacttgtttc actttgctga tcgtcttcac
    11401 atactttcgc atgcttttct actggatagc tttcccctgt caatgctgct tgagaaatga
    11461 atagatcctt tgcggacaat aaacgcatat ccgctggtat catatctcct gctgaaagcc
    11521 gaacaagatc tccacagacg atttcttcca taggaatctc tgtatactcg ccatttcgtt
    11581 tgacagctgc cgttactttc acaagtgatt tcagtttttc agctgcttga ttagaacgca
    11641 cagactggat caacgtcatc gtcccactga cgataaccat agtgaagata atggctacac
    11701 caaagagatc tttatctcct ggagcggcta atacgtattc cgtaatgaat gaaataattc
    11761 ctaatgcaat caacactaat gtaaagggtg tgatgtatgc tttcaatatt tctataatga
    11821 atggtgtttt tttgccatat gaaatcgcat tttcaccata agtttctctt gaacgctcga
    11881 tttgttcttc tgacaatcca tttggtgatg catggaacaa ttcaaataat tcttgcttcg
    11941 tttttccagc aaaagctgca tagatttttt gtgtatcttt ctttttttcc atctcgatct
    12001 ctcctttgtg tccaaaagac agctgttctc gctgtttttg gtcttttgct attcatcgga
    12061 tcgatagaga aaatgctgaa aggggctgag aacagataga taatagccct ggcctttcct
    12121 ctaaatcctt gtttttcgaa tgactgtcat ccatttgttc tcatctcctt tactaagtaa
    12181 ttttaattca tgacttccaa tcatttttcg aatagttatt ttcccaactc gctcacctcc
    12241 ctttttgttt tttcacctat gggatttgcg gcaacaaaaa aacctctcgc caaatactca
    12301 gcgaaaggtt tgaaaaagca aatcaaaaaa agcttctcag atctcatcgt tgagttttga
    12361 cactgtacaa cgtaaaaagc agatatctgc ttttagctac cttatgaaaa gccttatctc
    12421 gacaattcct gttctaccca ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg
    12481 tataggagcc tcacctaaca aatcctattc ggttttccct cgttaatata acaactaaaa
    12541 aagaggatgt caaatcaatt ttccatgttt ttcatacttt tttaaaatac acgcgttata
    12601 tttcctgtct cacgcagttt tatttgtctc attttccacc tcccgctata atagaattat
    12661 agtaggaaac gaggtgctgt tatggaacga acacttgtta ttatcaagcc agacggcgta
    12721 agacgccatt tggtcggaag catcatccaa cgttttgaag caaagggact ggccattgct
    12781 gaaatgaaat ttgcaacgat gacacctgaa ctagcaaaag aacattatca gcacttgact
    12841 gaacgtagtt tttttgatga gcttatcgat tacatgactt ctggtccggt cgtttatctt
    12901 gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga tggtcggagc aacaaaaggg
    12961 gcggatgccg ttccaggtac gatccgagga gattatgcac tgccggggac tgaaaacatc
    13021 atccatgctt ctgattcccg tgatgctgca acaaaagaga tcgcccgctt tttcccgcca
    13081 ttcgatacaa acaaaccgtc tgaaaaagcg gcgaagtgat acaaatccta aactgttggt
    13141 ctaactgctg ggtttagcaa tggccagcag ttttttctct aaagaattcc tttgattctt
    13201 ccctaatgta tattcgatca ctgcgaaaaa ctcttggtga ttttccgatt tttcttcccc
    13261 taaagcagca gaaatcagtc gttggtaata atccgccatc aatgtttcat aaatcactgt
    13321 acttctttct ttatgcgcta aactccgttt catatcttca ttcacgctac gtgccttttt
    13381 taagcgacca ttttctatat aaaacaaagt caagttcgtc aaaaaagtaa agagatactg
    13441 ctcgtgatta ttcagttgcg cgatccgtcg cattcttttt ttgattttca aataattatt
    13501 ctcaataaaa cgatcactga atatccaagg aaaatctgct agaatagata cttcaaattg
    13561 cccatactct ggtacttgca tcaaatattc ttcaatgata cgacattcat aacgcaaaaa
    13621 ttcaaattcc gtctcagata cttctgtttt tttggacatg tgcagattca aaaccaaata
    13681 gtacagatct tctgttttct tatagtgcct gcggttatta gcaatttctt tgtaaggatc
    13741 aaagctgtct tcttgaatcc gatctagcat tcgtcttttt ttctttaatt tttttggctc
    13801 atcaagctga tttcgtatcc attcatattc ggcaagtgtc atgttcattc gctctaaaaa
    13861 tgatttcaat gttccaaacg atactttttt cactgaattt gtctcgatcc cgactagcga
    13921 agttttagaa cagattcctt ccgctaattg ggtctgtgtc agctttttgt tgactcgaag
    13981 cttcttaatc aattctccat catacataat agttctcctt ttttaattga aattaaccct
    14041 tggcttttcc agaatgcttg ccaataagag gaatgattat ttcaatttaa attttccgca
    14101 ttcaataaat agatacgtta aaaataaaaa atacacgaaa aaaataaaaa aatgtttttc
    14161 tcataaaaaa taccaacctt caagcatgag actgctgtct aatccctgaa ggttggtttg
    14221 attgtttatt gatcattttt ttactgctcc gcattggttc ctgttactgg atataggata
    14281 gtcgttgttt tattgccgtt ttttgaaaag acactggtcg aatgttcacc tttttgcttt
    14341 tcgattgctt cattacgttc cgcttcgttt ttatagtcat attttttcgg atcaatcgtc
    14401 tcaaatccgt ttggcacata aaaccgcaag agattttcct gattcagctt atctgaaaga
    14461 cttaacgcag tcttgacttt ttcctgatct ttggagattt cctcgtcgat tcctgctgct
    14521 ttcgtatcga tgatttctcc tgtctgatta ttgtaagctt ttcctcctag gacggtgtat
    14581 ttgggcgtca cgaagttccc atttcgaaaa gcaactactt gatcgtgctg agaagataat
    14641 aagtctgtac caaaatgaag ataatcttta tcatcgattc ccaatagatg tagtaatgtc
    14701 ggcaaaacat cgatttctcc accatattca tgattgactg tccctttagt gtaaccaggc
    14761 atatggatca tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct
    14821 ggatctttac caagagcact tgctaaatct ttattatctg tattcgagat cccaaaatga
    14881 tccccgtaaa tcacaaagat tgaattttgg taaataccag attttttcaa atacgtaaaa
    14941 aattgttcca atgattgatc cagataatgg gccgttcgaa cataattatc tactgtgcta
    15001 ttgccagtat tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg
    15061 ttggttacgg aaagaaattt cgtgtaaaac ggctgttgta gatgttcaag atattgggca
    15121 gattctctga ataaatcttt atctaatatc ccataaccta acgtttcatc ggattcatca
    15181 aaataagatc gatcaaagaa attgtcatac cctaaatttt tatatacatg atctctattc
    15241 cagaaactag ctacattccc atggaaaaca gcacttgtat agccttctgt ttgaccaagg
    15301 gtcgctggtg ctgcttgaaa gacattatct gacccaagtt ctgtgaataa tgagccttga
    15361 ggtaaaccaa atgtgccagt ttccaacatg ttttcagcat cacttgtctt tccctgtcct
    15421 acttcatgaa agaaattgtc aaaactgatc gttgcttgat tctggaaaat actatttaaa
    15481 aacggcgtga cttcttgtcc gtctaccttc atattgatca aaaactgttg aaaactttct
    15541 aaatgaagga caatcacatt ttttccttta gcaatcccaa atgtttcagg atttggctct
    15601 gcataatgtt tctttgtata gtctaaaact tcatcaattc cattactgct tgcatgcgcg
    15661 cgaacactgc tagtcattcc tgttttgatt ccgtcataaa tagtgtacgc atcgattcct
    15721 agatatttga caatatagga acggtcaaat gtccgctgta gcaattgggg acggttggct
    15781 tcgctcaacg ctaaatttcc tgaaaaaatc aatcctgaaa gacaggtaac agcgatagcc
    15841 atcggtttgc tgacagggtt gcttttgata ggtattttct tgaccttcaa ccaaaccagt
    15901 agcccaatga acacagcgat atccaaccaa tagatgacat catggggttt catcaaagaa
    15961 aaggagcttc ctgacaaccc ttgtgacact ttcgaaaagc ctaatacaga tttgactgta
    16021 atgaaatctg tgaattcccg ataaaaaata atattcaagt acaacaaaag cgtatttaaa
    16081 atatcaatga tcaaaagaat ggctaacgct ggctttggct ttttaatata caaagaaagg
    16141 ccaaaaaaca ataaggtagt cgcaatcggg ttgatccaca agataaagta ctggatcgta
    16201 ccttccacac ctaatgaaaa atctgcatag taggcaatga tcgtcttcag ccagaaaaag
    16261 aaaacagctg tcaaaacgat tgctaaacga ttttggcaca attttttaag ttgtgtcacg
    16321 tatatcactt ctctctattc acattttttc ttattttgct cctgaaaaat gaaaatttcc
    16381 taaaaaagtt gttatcttaa gattttttta ttctttctaa agattattcc attcattttt
    16441 cacagcataa aatcgtttcg gtgtaagcgg ttaaaatagt aaggagataa aaatcttgtg
    16501 gtgaccgatt tactctatag tattcaaaag tagtttctcg gtattcgtac aaaaaaaaca
    16561 tactaagatt agaggggaac atcattgacg aatgatgttc tcctctattt tctattttta
    16621 gattatagtg aagagtgaaa gaaagttcga acaaagcctt gaggactacg agtccctatc
    16681 aaaaaccgaa cttctttact gtcattttag aatcaggttt acgtatgttg gggccatgag
    16741 cccgtgtata ggaaattgga atcgaaatga agcagtaaag ttctttcaac acttaaaaag
    16801 gaggaaaaca aatgccttac attcttgcat tagatgtttc aatgggacac agctacagtg
    16861 tcctatacaa agacgacacc tgcttatttg aagacgagat cgaacacact caaagaggat
    16921 ttcatgcatt actcgaagag atccagcact tgcctgaacg tccattgatt gtttttgaag
    16981 caacgggtat ttattccaga ccagtcgaaa agttctttca agatcatcac ttttcttact
    17041 gtttgttaaa cccgttagaa gcgaaaaaac agatggaaga aaacacctta cgcagttgga
    17101 aaacagataa atcggatgcc catcggctcg cacagactca tcttaaaaat caacgtcaac
    17161 caaaagaagt acaaagcaat ttttacttag agatgcgcga tttagcccgt ttttatcaag
    17221 aaattgagaa gaaaatcact cgactgcgca tggatttaca caactgcctt cagttgactt
    17281 ttccagagct tgaacagttc ttttcaaaca ggctgacccc ttacgcattg acattgatcc
    17341 gtctttttcc ccatccagat tttgtattgg catctactcg gacaaaaata aaaaataaac
    17401 tgatcaacga aacacgggaa aaaatctccg cgaatcgtgc cgagcaaaag gcagatcaaa
    17461 tcatccacta tgcacagtgt gcctatccag ctgttgaaaa agacagtatt cactgtcaaa
    17521 aaacgatcta ttacgccgaa cttctccaag atttactcga acaaaaaaaa gcactggcga
    17581 cacaaatgat caaaaaagcc gaagcctctc cttgcttttt gttgtaccaa acgttccctg
    17641 ggattggcgc actcacagcc gctttattgc tcggtgaatt aggagatatc acacgattta
    17701 aaacccataa acaattgaat gcctttgtcg gaatcgacat ccgacggtat cattcaggta
    17761 agtatactgg ccaagaccgc atcaataagc gtggtaatcc caaagcacgg aaaattattt
    17821 tcttcaccat tcgaaatatg attcgccagc aacgcgcagc tcctaatcat attgtcgatt
    17881 attactacaa attaaaaaag caacctatcc ctaagaaaga taaggttgcc acagttgctt
    17941 gtatgaacaa gcttctgaaa tgtatgcacg ctatggttag ggcacataca gagtacgact
    18001 atgcgtacgc ggtctcagtg gaccattaac taaaccactt acagtatacc acttctttgc
    18061 ccagatcgaa aattttttac gaacggggct ttatttagtg tgccttgatt ttgtcgcttt
    18121 cgtttacgag ctatttttct tgcacctttt acttgactaa tcgtaggaaa gaggctgtga
    18181 caaaattctt gtcgcagctc cctatttctt gatgcaggac agctttttca caacctctcc
    18241 actaaacggt gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag
    18301 gagctatttt cccgtttttt tctctaatac tcaggtcaaa ccgctgattt cacaacctct
    18361 gattcacggt gttccagaag tagcttcttc ggaaataagc cgaaatttta caaaaacttg
    18421 agaagcactt ttcgtaaatt tcttcttatt tcttgaagct gaccacttct gtcacaactt
    18481 ctgtttttca ttgtccatag gtttgatatt gtccttctga tgactcattc gtaatgattg
    18541 aggcttgatt ttcttcattt cccgttatcg tttgtccact gccagaatct gaagggctta
    18601 gttctgtcga ctcgtttttt tgagaagaag cagttgtttt cttgacttgt tcgttggtct
    18661 ttttccattc cgctagtcga tcggccacca cttttttgtc cactttttca gaataagctg
    18721 taaccacttt tccatttttc aaacgtgcta ccttgtcgcc atcatgagtc aaaagcaaaa
    18781 cgaccaaatg tttgtctttg aattgatatt gtctgagaga aatcgcacta agtacgtcat
    18841 cgatattgat aaattgattt tcttcttttc cttttggcaa atagatgtcc aattcacctt
    18901 tttcgtaatt ggcagttaat ttatattcct cccaccaata agttgagaat tcttttttga
    18961 aattatctac tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg
    19021 ctgtcatttt tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc
    19081 aaaagagaag aaacggtctt tttttcctca tgccttcctc cactcctatt taattttgac
    19141 tattctatta tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat
    19201 aattgaactt ctttttcctg aagcggtaag aacgggaatt ttcatacatt ctctcttggc
    19261 tattctttaa gcttcaaatc catttctgtc tgatacatct ttgaaccaat gacccgattt
    19321 cttgatcgtt ctttttccat cttgtgctaa atctactgaa ataaaaccgt aacgattctt
    19381 ataggcattg gtccacgacc agttatccat gcatgtccac atatgataac ctaaacaatt
    19441 ggcaccttct tgaatggctt gatggacata tttcagatga gattgaacaa attcgatccg
    19501 ataatcatcg tggatcatac catcttcctt gataaatcgt gcttcatttt ctacacccat
    19561 gccgttttca gaaataaaac agtcgatatt cccataatga tctcgtacat tgatcaaaat
    19621 atcatagatt cctttttcat agatctccca tccgcgataa ggattcattt tacgaccagg
    19681 catttcataa ttgtcgaagt aatcctcagg cataggaccg tttttagtat cgattggtgt
    19741 ttcttttgct tttattcggc gtggttggta ataattaatt cccaaaagat ctactgtgtt
    19801 ttctttaatg atcgtcaaat catctggatg aatctccggc attagatcga gttcttttat
    19861 tctctcaatc aattcactgg gaaactcgcc ttttacagct ggatcaagga atgaacgatt
    19921 gaagaacgca tctgctattc gtgctgcttt aacatcttcg ggatttgatt cgtctcttgg
    19981 ataactcggt gtcaaattta agatgattcc gatcacaccg ctttgatcca tttcatggta
    20041 agctttgatg gctaatgctg aagccaacgc ttcatgataa ccaacttgga ttgccttttt
    20101 catatctgat tcttccggat aatgccatcg atacaaatag cctgcctcga ctggaacgat
    20161 tggttcatta tgggtaaacc atttttttac tttcgagcca aacaattgaa aacaactttt
    20221 tgcgtatttt tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag
    20281 atacatcggc atatcgaaat gatacagatt aacaaagggt tcgataccgt tttcaatcaa
    20341 ctcatcaaaa tagtgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc
    20401 aggtatcaaa cggctccatt ggatagaggt tcgaaatgaa ttatgcccag tctgtttcat
    20461 caactgtaca tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg
    20521 atcaaagaac ttttgcggtt cgtttttgta ccaataatca aaaatattct ctccttttcc
    20581 atcgcctgaa attcttcctt ctgtttgtgg accacttgct gcagagcccc accaaaaatt
    20641 ttttggaaat tgatatcttt tcatcaaagc acctcttttt cttttttagt tgattccatg
    20701 tatgatattt tccatcacaa tcactttttc attttcttcc tcttcttttt tgattcgttt
    20761 caacagctgt tctgctgctc gttccccgat tttatctgtt ggtggcgtga tcgtgatttt
    20821 tggaaggata ctcagatcgt ttttatctaa ataatcatag tgactgaacg caatatcctt
    20881 cccgattctg acaccttctt gattgaaaca ttcgatggca ccaatcgaca tgttgtaatt
    20941 gctgacaaaa atagcagttg cccctttttc taataatgct tttgccccgt aatagccgct
    21001 ttttctagaa tagtctcctg taaagatctc taaatggata gacggatcct tttgtaccgc
    21061 ttctttggct ccagctaaac gttctcttgc tacgtaatct gtttgagcgg cagcaataaa
    21121 tcctattgtt ttatgtcctt gtccgccatg atgagcaatc acttttgcta cactttctcg
    21181 atcattcact actattgtat caatacctgc tggcccaccc ggacaattca atgcgacaac
    21241 tgggatactc atttttgtca gcatttcaaa tccagaccac tcttcttctg ataagaagac
    21301 gatcaatcca tcgatcgcat gcttcatcag gtattcgatt ttttgttcga ttttttctgg
    21361 ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta ctctttcgat
    21421 ccctgaaaca acctccgaac caaatcggtt agatatcccg tttatcaaca gtccaataga
    21481 gaaacttcga ttattcttca atcctttagc aatgatgttt tctttatagt ccaacgctga
    21541 aatcgcttct gatatttttt ccatattctt ttccttcagc tgctgaccgt ttaaatagcg
    21601 ggaaactgtt cccactgaaa caccagccat cttagccacg tcacggatcg atgccataca
    21661 ttttcactcc tgccagttac atttgatttc tttttcttgt tgttcgacca tgatgaccgc
    21721 tttctttgca taatgccgct tgtcatcaat atccatataa ctaaagtctt cagaagtgag
    21781 ttcaaaaaca agcagtcttt cctcttgaga ccgcaattca tctttacgaa aagctttcaa
    21841 taatttcgtt ctttggatga caacgccgtc aattaatcgg acatagacaa gagtacttgt
    21901 tttacctgaa acacttcctg tatttttaac tttaattgat attattactt tgtcaatatc
    21961 cgttttgact tccatctgac tgtattcaaa tgttgtatag cttagaccat gtccaaaagg
    22021 ataaaatgga cttccgctta aatcataata attctcttgt ttagaagcag cacgctgata
    22081 gtaataaaca ggtagttgcc gactatttct aggataagac aaactcaatc gtccgcttgg
    22141 gttatttctt ccagaaatca tctgcgcaat ggcttttcca ccctcctgtc caggaaacca
    22201 tgctgttgca atggcattcg taaatgactc gattttcatc aaatcgtagg gtcttccttg
    22261 cacaagtatc gtgatgattg gcttgcctag ttgatacaat tgttcaatca gctgttcttg
    22321 gtatccacct aaagaaagag aagcaacatc tacattttct ccagaatcca tgttgatccc
    22381 tttcgatgaa actgccccgt tacgaagaaa ttccatatca aagttccgcg cacttgatcc
    22441 acctagaact gcaatgatca catctgagtt ttgagcaagg gtcaccgctt ctaataattt
    22501 ttcttcctga tcctcctgat ttcttacttc acatccttgt gcataagaaa cctttgattg
    22561 gggaaactct gtttttatct cttgtactat cttttttttc aaacggtctt ctgtttgtgg
    22621 tgcagagtag tctcccaaca agtggtaaac atcgtgagca tttggaccta taacagcaat
    22681 gttttttact tgttgatcca acggcaatat accgtcattt tttagtaaag tgatactttc
    22741 ttttgctgtc tctaaattta agtgctctga ttcctccatg agatgatccc aatactcctt
    22801 tggatcgttt gcaaatggct gttcaaataa acctaataaa aatttgacag ataaaactcg
    22861 gcgaactgct tgatctagta gcttttcatc tacgacttga ttatcaatgg cttctgcaat
    22921 cttggtatag gtctcatccc acaaactgag atcgattcct gccgctaatg catatgctgc
    22981 agcagtcttt ttatctgtaa aaacatcgga taaacgatcc aaagccacac cgtctgccat
    23041 aacgattcct tgataaccga tctcttttct taatgtttgc tcaaaaagcg ctcgattgat
    23101 atgacaaggt atcccgtcga tgtcattata agcagccatg acacctactg catttcttgt
    23161 actcttcaat aatggtccgt aaatatcgga gaactctctg cgtccaatcg ttactgtgcc
    23221 tgaattatga ccgcctaaag cttctccttg tgcgatacag tgctttaaca caacaccgat
    23281 ctgatccttc ttcttattga tttcctctac tgtctgatca agaaaagaaa ctttttctga
    23341 aataatctct ccttggaacc ctcgaacgac cgcttcagaa aaacgagctg agagaatcgg
    23401 atcttcccca aagcactctt ctgtccttcc ccaacgagga tcttttgcca gatccaaagt
    23461 cgacaccagc gctaaatgaa cacctttagc agccagttct tttgccatca atcgactcga
    23521 ttgttctaat aatgctacat tgaaagtatt tccacggccg atattcgttg gataagaaat
    23581 cccatccaag ccttgatgac catgtggaca ttcttctaca agcaaaactg gaattttcca
    23641 tcgcgaatgc tgtagtacat attcttggac taggttagcc accttccagc tttcttttgc
    23701 aggaatccca ttcttataat ctgttttaga ccacggatca gagcgaaaca gaccgtataa
    23761 ggcacccaga ccgcctcccc atttaacatg ttcttttaac ttttctgtta attcgatctt
    23821 gtctccgttt ttttcataac attcccaacc atacaaatgc tggtttacct gtcctacttt
    23881 ttcagcaata ttcatcctcg aaagtaaatc ttctactctt tggcggatgg ttaactttgg
    23941 gttttgatac gggtaatcgt acaccttaat atcctccttc tgccaatttt gccttattag
    24001 cggctaatac aaatggcagg aataaaaata catctagaat gataagtatt actgttaaga
    24061 ctgctgctcg tatatcgcct cctgtagcaa taaaagattg tataaccggt ggagtgatcc
    24121 aaggtgcagc agcaactgtt ttactgatga ttccaagact tgtggctgcg taggcaataa
    24181 ccaagttaat agatggtata atagcgcatg gaatcatata tattggattc atgactactg
    24241 gtaatccaaa cataattggt tccgtaatat taaataaaca agtagtcaga ccaaatttag
    24301 cgattgatcg ctgttcttga cgtttagaag cccacaagat agcaataact aacggtaaaa
    24361 tacacccgcc acctccgatc aatccaaatg cattggtaaa gggctccgta ataatattag
    24421 gaatttcttt tcctactgaa aaagcatcca tgttttcctg aatagactgt aacaaaggtg
    24481 cttgccgtat aggagccaaa atactcgaac catgcatacc aaacacccat aagaaatcag
    24541 agaaaaactg taaaaataag atgcctggga cggataaaac aaaagctttc aatggtgtct
    24601 gaatcgctaa ttcaattaat tgcggaacat tcaaaccagt cgtatggttt aaagtaaaaa
    24661 cgacaactga aaaaaataaa atgacaacta tttctggtac taaagaatta aatgacgcag
    24721 ctactgccgg tggaactcct tctggcatcc tgattttaaa actcttcatt ttcgctaatt
    24781 tgataaataa ttctgtactg acaagggcag caatcatcgc cgttgctaag ccagacgaag
    24841 aagtcagact ttgtaagaaa acccctgtag cttctgctgt atttccgtcc atcaaagtga
    24901 ctgtacttga taaagacatc aaaataaaca ttacggctgt acttagaatt cctgcatgaa
    24961 caggactaaa tgatggatca tcaatcacgc ctgtttggat atagtgggtt gccaaattcc
    25021 agccgatatt gtaacacacg ataatagcta aaatactcat cgtaccatta ttcacgattg
    25081 aagcaagttc agccaatgga cttagatcaa gtattctttg gactgttgca tttgaaaaaa
    25141 tcagagcatt cagcaaaacc atgacagaag cagccattac taacggcata gtaacgataa
    25201 aactgtctcg aatggccagc aagtgtcgct ggctactcat cttagatacg attggtagca
    25261 ttttttcaga tagtctgtct acgactttca ttatttcatc tcctagattc taaaattgaa
    25321 ccggttcatt tttaatatag cacttttttt aaagattgca acacattttt ttaaaacgct
    25381 ttctaaaggt ggtggatttt ttatgacttt tgaaaaatta ttctatttaa tggtattttg
    25441 tgaatttttt ctcttccatc cgtttgaagt tttactttcc aatggtataa tttctttatc
    25501 ctatcaaatg ggggaagtat tttgaagaaa ttaatcgtac tatttatttt gtctgcggga
    25561 cttttgacat tgactggctg tgatttgttt ggcggctctg accaatcctc ggacgaaaca
    25621 accgattctt ctgtcttaaa agcggaaaaa gcagatatca ccaacagtgt atatacgcta
    25681 aaagtacgta aagatatgga tttatccgcg ggacagttga catttacaga taaaaaaatg
    25741 gaatggacac gcacgtatca gacacaagga gctgattctt ctgaatcatc tgaagaaaaa
    25801 acagtgctga cagatatcaa gattactacc aaagatgacg aatatattat ttcaggaaaa
    25861 gaaaataaaa aagaagcaac agtcacattt accaaaatcg gtaattaccg tttgaaggat
    25921 aaggatggca acatctacag tctatagtgc catctgttgg atcttctttt gtttctcccc
    25981 ttcttttctg ctatagtaaa gaagcaagat gactgtaaag gagacgaaat ggatgatcga
    26041 tagtcctgat tattgtacat gtaataacaa caaaaatatc catacagaat ttaatgatca
    26101 aggaagtttg agtatatgcg acaattgcca caaactagtc aaagattctt ttaaatcgtt
    26161 tgaatcttat cctgtcaaaa attatgtaaa aagcaaaaga agataagcaa aatcaagcaa
    26221 aactcactat ggctgatttt tatgaacttc aaactcagct tcttcctttg atcaagcttc
    26281 aaaaactgga atcagtaaaa caaacagtgg atcatttcag ttcacaaatc aaagagtaac
    26341 gttttattta tttcacatct ttgaatataa aataaccaaa cgatagaaag acttattcta
    26401 catcacgtag taatttttct ttcccgtcgt ttggtttttt acttttagcc taattcttga
    26461 cctttcgcat gctgattatc tgtaaaatat tttccagcag gctttccttc caattcagct
    26521 aatttttctt ggatctgacg aatcaccttt tttgcatctt ctggattttc tacaaaatcg
    26581 aatgtatccc catccacttg tattttagga catacatcaa aatcctcata ccattgatta
    26641 tagcgggtgt tcagtgtttc gtaataggtg tataacgttt catcgctttc tagctgttca
    26701 tagtcacgcc cgcgtttttt gatacgttct agcatcgtgt ccaaagaaac tttgatatgg
    26761 accatcaaat caggacgttt ttttggagca acatcatcta actcgtttag catcgtatct
    26821 aataagctat cgtactgctg tacttcgata tccgttaccc gacctaaatc agcatttaga
    26881 tggaacaaca gactatcttc gtagatggag cggtctagca cattgtcatc gttcactaaa
    26941 gcatttttca ttgctaaaaa tcgcttattc aaaaagaaga tttgcaataa aaatgcgtac
    27001 tgctcaggat ttgaataaaa aagcggcaat acttcgttat cttcgacatt ttcataaaat
    27061 ggtttagaat caatctcttt tgcaagcata tccgtcaaac tgctcttacc tgcaccaatc
    27121 gttcctgaaa tcaaaatcac tgacatacaa cccttcctct tctataagta ctcgcctacg
    27181 taagtttcaa caggcaattt ttcaaaatca tattcacgta cagcttggtc tgattttaaa
    27241 tacgtgtgaa gactttgtgt aatcatcaag ttcgtaggta ccgtctcttc ttgtacgatg
    27301 atcatcggtt ttttcaacat agttgccaca cctaattcgt aagccgttcc tggatcgatt
    27361 gtttgatgct caaagtcaag gatagccaaa acaaattctg catttgtcaa ctcttccata
    27421 tctttttcgt atacttcctg cgcccatcca gcgctgaata gttcataatt gctttcttgg
    27481 tgatgacgtg gagagtagaa acttgtgatt gttttgtttt cctccaacgc tttttcgata
    27541 cgacttactc gatcgatttg ctcctctgaa aaaaatggtc ctgctaaata tatttgactc
    27601 attattttgc ctccttcaat aataactgat ttttaataga tactgctcgt aattcattga
    27661 ccagttccaa tggattgttg gaaaagatca cttgatccaa ccatttttta tccataaaat
    27721 cctcttttgc aaaagtttct aataattgtt gcaagccgct gtaataaccg ccaatattca
    27781 atattccgat tggcttatca ggtgtcaggc cgatcgctat ccaagaaaga agttgcgaaa
    27841 attcctccaa tgtacctaga cctccaggga aaacgaggac agcatctcct ttttcaaata
    27901 ataatacctt tctttcatca attgtctctg taggaataaa tgtagtaaca tctttcccag
    27961 gcagctcagt ttcaaaaagg ccatttggat agatacctgt gacttttccg ttattttcca
    28021 gtactgtttt actaatcact cccataatcc cagagtatga accaccgtaa accagttcga
    28081 tttcttcttg tgccatatat tttcctaacg tttgcgcaat aaatttgtaa ctttctttat
    28141 tgccaaaacg ggaaccgcaa aaaacagtaa gtttcataaa aaaacactcc ctaactcaaa
    28201 agtctatctt cattctacac gtgttttttt aaaataaaag actagatttt gtagtttttt
    28261 agtggtatat
//
LOCUS       DAEGIQ010000028        27872 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000028 DAEGIQ010000000
VERSION     DAEGIQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.028"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(378..1322)
                     /locus_tag="KMT45_001092"
     CDS             complement(378..1322)
                     /locus_tag="KMT45_001092"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBE7004565.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1618..2562)
                     /locus_tag="KMT45_001093"
     CDS             complement(1618..2562)
                     /locus_tag="KMT45_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7004566.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2783..3997
                     /locus_tag="KMT45_001094"
     CDS             2783..3997
                     /locus_tag="KMT45_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7004567.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4019..6034
                     /locus_tag="KMT45_001095"
     CDS             4019..6034
                     /locus_tag="KMT45_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBE7004568.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6117..7490)
                     /locus_tag="KMT45_001096"
     CDS             complement(6117..7490)
                     /locus_tag="KMT45_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBE7004569.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7516..7800)
                     /locus_tag="KMT45_001097"
     CDS             complement(7516..7800)
                     /locus_tag="KMT45_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7004570.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7802..9817)
                     /locus_tag="KMT45_001098"
     CDS             complement(7802..9817)
                     /locus_tag="KMT45_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBE7004571.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9996..10301)
                     /locus_tag="KMT45_001099"
     CDS             complement(9996..10301)
                     /locus_tag="KMT45_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBE7004572.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10390..11421)
                     /locus_tag="KMT45_001100"
     CDS             complement(10390..11421)
                     /locus_tag="KMT45_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE7004573.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11628..12503
                     /locus_tag="KMT45_001101"
     CDS             11628..12503
                     /locus_tag="KMT45_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBE7004574.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12655..13497)
                     /locus_tag="KMT45_001102"
     CDS             complement(12655..13497)
                     /locus_tag="KMT45_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBE7004575.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13589..15466)
                     /locus_tag="KMT45_001103"
     CDS             complement(13589..15466)
                     /locus_tag="KMT45_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBE7004576.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15463..16884)
                     /locus_tag="KMT45_001104"
     CDS             complement(15463..16884)
                     /locus_tag="KMT45_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBE7004577.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16887..20276)
                     /locus_tag="KMT45_001105"
     CDS             complement(16887..20276)
                     /locus_tag="KMT45_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBE7004578.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20533..21924
                     /locus_tag="KMT45_001106"
     CDS             20533..21924
                     /locus_tag="KMT45_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7004579.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21997..22644)
                     /locus_tag="KMT45_001107"
     CDS             complement(21997..22644)
                     /locus_tag="KMT45_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBE7004580.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22652..23338)
                     /locus_tag="KMT45_001108"
     CDS             complement(22652..23338)
                     /locus_tag="KMT45_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004581.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23344..24417)
                     /locus_tag="KMT45_001109"
     CDS             complement(23344..24417)
                     /locus_tag="KMT45_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004582.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24639..25313
                     /locus_tag="KMT45_001110"
     CDS             24639..25313
                     /locus_tag="KMT45_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7004583.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25318..26658
                     /locus_tag="KMT45_001111"
     CDS             25318..26658
                     /locus_tag="KMT45_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBE7004584.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26807..27793)
                     /locus_tag="KMT45_001112"
     CDS             complement(26807..27793)
                     /locus_tag="KMT45_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBE7004585.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8127 a         5262 c         5050 g         9433 t
ORIGIN      
        1 tttttccatc aaaatctgct tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt
       61 tttttgtcgt ttaattgaat gataaaaacc agtttcttta ttcagttaat caaatatgaa
      121 cgaattagat atgaatcatt gtatttgccg ttgcggaagg attttttaat taaccacaga
      181 taaacattga taacgattcg cttattgtaa tcaacagatt aaatttcata tgttcatatg
      241 tacggataca ttttcggacc gattaatttt aaccattctg tttatttgct tacctattag
      301 atatcaataa aaaactgctt aacagctatt tcagctgtta agcagtttta ggatttttag
      361 ttcatattca ttgtttctta taatacagtg ttcaatactt ctcttaaagc acttgttgat
      421 ttgctgaaca ttgctttttc ttcttcgttg attgataatt caacaacttc acgaacacca
      481 ttttcgtcaa cgattgatgg cacacctgta aagatatctt tttcgtcgta ttcaccagtt
      541 aaataagcag atacaggcaa tacagcttgt tcgttattca agatagcttt tacgatacgt
      601 gcagtactca taccaatacc atagtaagtt gcttttttac gatcaatgat ctcataagca
      661 gcattacgga ctttatcagc aatcacatct aattcatctt gggcaatacg atggtctttt
      721 tctacaattt caaacactgg tttaccaccg acagttgtat gtgaccatgc agcaacttct
      781 gaatcaccat gttcacctaa gatatagccg tggacgctgc gtgggtcaac ttttaatttc
      841 aatgcgattt ctttacggaa acgagttgta tccaatgttg ttccagtacc gataacacgt
      901 gatgttggca gaccagattc gttccaagcg atatatgtca aaatatctac tgggtttgaa
      961 gcaacaacga taataccgtc gaatcctgat cccatgattt cttttacgat ttggcgcatg
     1021 attgacgcat ttgttttaac taaatctaaa cgagattgac caggtttttg gttgatacca
     1081 gctgtgataa caacgatatc agcatcttta cattcttcat agccacctga ccatacagcc
     1141 acattttcat cgccccaagc cataccatct aaaaggtcca atgcttcccc ttctgctttt
     1201 tcttgattca catcgatcaa tactaattca ttagagattc cttggttgat cattgcataa
     1261 gcgatacttg ttccaacaaa acctgtaccg acgattacta ctttacgact tgtttttttc
     1321 atgaataaac agctcctttt cataactatt tgttactttc atgaaaagta tagtcttgtt
     1381 tattcatttt atcaacatgt taattataat agttgttatt ataatttttg atggaaacga
     1441 attcctgaat acgcataaca gataaatgac aaatgtttac acctatatcc cctgataata
     1501 caaccaaaag tttagaactg aaaataaatc caaaaaaatt cgctgcaacg atataataag
     1561 acagcaatcg cacctaaaac aattttcctt catatgcttt agtgaaagat tttcttctta
     1621 atgtttattt ctccattcat tgggagtaca tgttgaatac ttcttgaata atttgaataa
     1681 agaagtcgtt tgtttatatc cgatacgatt agcgatttct tccaacgaaa aactcgtttc
     1741 ttgaatcagt tccaacgcaa agtcgaattt tttctttaga accagttctt gaaaggaagc
     1801 atcaaaatac attttcaatt tattactaag atagaccgga ttgtagccaa aatgagctgc
     1861 tgtttctttt aacgtacaag aagtaaaatg cttatcgata taatctaata ctctaagcat
     1921 ctcattttct gcgcatccat ctgctgatga aaagctttgt tgttctaata aatgagtcaa
     1981 ttcgatcagt aatagtgatg tatagagatt taacgaacgg atcttatgac tgtatgtatc
     2041 aaaataaatc aacagcatat tttcaatcaa atgcgtcaaa cgacttgttt ttccgccgcg
     2101 aaaatgaatg aattgtgtat gttccccttt ttcttttgat gcatttatca aaaaagaaga
     2161 aagtagatta tttgtgcttg ccaaatgatt caatatctct gtagataagg tttcttcttt
     2221 taataagatg ttgattaaaa tatcctcttg ccctgtgtat tcgattcttt gagtagcttc
     2281 tttatcaatg atcaccacgt cactttcttc catctctatc ctttctccat taataatttg
     2341 taaacatgta ccgctataca tataattgat ttcaacaaaa tcatgtgtat gctcgggaac
     2401 ttccgcatat cgacggtgtt tactgatatg aatatttccc tgttgaaata gatcccctcg
     2461 gtggattttt ggcggtgtct gaagatggta agctaagttt aaatcattga tattcattcc
     2521 ttgttcacgc tgcagagttt cgattgattc ttcgttacgc aaaaaaaatg tctaattcag
     2581 ctagttccaa ttgactccct cctttttgta agcggtttct ttcgcttatt agttcacgct
     2641 gtactgctat tatatgcaaa gctatttttg gtttcataaa gactttctat ggttgggaca
     2701 tgaacttcct gtgattccgc tattgagaga ataaccactt catactatat ttttgataag
     2761 cgcttacaaa aatgaaagga acatgcttat gaataaaaag atgatgaaat tttctctgct
     2821 gtcagtctct cttattttga cttctgctgg tgcagtcacg tctgcaattc ccagtatgtc
     2881 agctacttat gccacgcgtt ctttatctgc tgttgaatta ttaacgactg ctccctcttt
     2941 tatggtcact atttttgttt tgattagtag ttttattgct aggaaaatcg gacaaaaaca
     3001 aactgtgatc atcggattag tgattgcatc gatttgtggt atcctccccc tcttctcaca
     3061 aagttacagt attgtcttag cttcaagagc tggactaggt attggctttg gattgatcaa
     3121 ttctttggca gtcagcatga ttgctgcctt ttatcacgga gatgaacgag cctctatgat
     3181 tggttttcaa agtgcatttc aaggatttgg tgcagcttta atgacatttg ttgcaggaca
     3241 gctagtacag ttcggttggc aatatacctt tttagtctac gccatcagct tacctattct
     3301 tgtcttgttt gttttgtttg ttcctacacc tgaaacaact gaacttcaat cgcatggcac
     3361 agccccaaca agacaagtaa tcaaaacgaa tcctaaaatg attagttatg gtatttttct
     3421 cttgctggtc atcgtggtat ataatgctgt atcgattaaa ttagccacag tcctgacgac
     3481 ttattctcta ggaaacgaaa ctaatgcagc gaatatctta acaatcatgc aattggcaag
     3541 tatggctgcc ggagtttgtt ttggctggct tcagacaaaa gcacatcgct atatgcttac
     3601 actttcttta ctactgatgg gaatcggctt tgtattgatt gccactgtga taaacctgta
     3661 tgtgattggg tttggtgcga tcctaacagg aatttctttt tcgttattca ttccttactt
     3721 attcaaccaa gtaagtatcc aagcgcccaa gtacgcggag agtttcaata cttcaatctt
     3781 attggttggc gctaatttag gtactttcat agcaccatat ggtttgctct ttctctcttt
     3841 tgcttctctt gggagtaaaa cacttgctgt tttcgtcaat ggcagtatct tgctgttgat
     3901 tagcgcgctt attagccttc tagtagtaca caggacgaat agaaagacag tgataagagt
     3961 agatgaagaa ataaaagaac aattgacaac aaaataaatt tgatggaggt cgtgtaaaat
     4021 gacaaatgaa aaacataaag atagatggct ttggtatcct ggagactttg agatccgcca
     4081 tggactgcta caaaatttcc aacgggaaga acgaggtttt gactggccag cgtactggta
     4141 catggatgat tgccatcgga acgtcaagtt taagagatat tactttcttg atcaaccttc
     4201 catgtttaaa gtgacgatcc aaggagttgg gtatgtagaa attaatggac aaaaacatcc
     4261 atgtggaaaa tggctgactt gtccagctgg taaagcaaaa attcgtatct ttgtaggaca
     4321 tacatcaggg ctaccagcga tgtttatcga gggagacgaa gtaaaatcag atattggctg
     4381 gacagcaagt aattttattg aagaatatcc agctggctgg agtccgcttt atgtcgatat
     4441 cgcaaaagat ccaaatcaaa tctattatca aaaagaatgc gtccatccag tcactgaaac
     4501 ggagattggc ggaggaatct tattcgattt tgggcgagca gtgaatggct gtgtagaact
     4561 aatatctctg gatgaccata tcgaatctat cactctatgt tatggagagt ccgatgtaga
     4621 agctatggat atcgattatt gctactataa gcaaatagga atgacaagaa aaatgaaagc
     4681 tcgtaaacgc gcttttcgct atttgtttat accagaggtt tctactggaa tggttcaact
     4741 tcaagttttt catgaatttt tatctctttc gaaacaagct gctttttcaa gcgattcttc
     4801 tttactgaat cagatctggt cagtatcaga agaagccttc aagttatgta gtgatttgtt
     4861 ttttatcgac ggtatcaaac gagatcgctg gatttggtct ggagatgcgt atcaatcatt
     4921 catgatcaat caatatcttt tttttgacga ggaaatcaat aaacgaacga tattagcatt
     4981 aagaggacaa aatgaaatca agcagcatat gaatactatc gttgactatt cgatcttgtg
     5041 gttgattggt atcgaaaacc actatatgat gacgaaagac tatgaatttt tacgcatcgt
     5101 ctaccctaag atgcaatcat taatgagata cttgatggag caaacgaatg aattagggtt
     5161 tatttatggg agagcaaaag attggatatt tattgattgg tctgaaatgg ataaagaagg
     5221 aacgcttgct gcagaacaag tcttattact gaaagcctat cattccatga tcacttgcgg
     5281 agatatctta gctgaaacat atggagagaa cgaaaattat catactcatc tctatcaaga
     5341 aaaatatcaa ctattgaaac aaaacctatt tgactacttt tgggatgaag aacttggcgc
     5401 ttttatcgat tgctatgaat ctgggagaag aaatgtgacg cgtcatgcga atattttcgc
     5461 tgttttattc gattttgcta acaaagacca acagcaatcg atcttaaaga atgtcttatt
     5521 gaatgacact atcacacaga ttactactcc atatttcaaa ttctatgaac aagatgcttt
     5581 atgcaaactc ggacagacga atatcgtcta ccaaacgatt cttgattatt ggggagggat
     5641 gttatctcat gatgcggtaa ctttctggga agaatacgac cctactcaaa caggaaatga
     5701 aaagtacagt atgtatggag atccttatgg aaaaagctta tgccatgcat ggggagcaag
     5761 cccaatctat cttttaggac gctattttat cggtttgtat ccaacaaagc caggttatga
     5821 agaatttatg atacaaccta atctagattc atttgaaaca ctgaattgta cgctacctat
     5881 caaagatgga aagatttcgc ttgaaaaaac aaaagataag atcgtgatta cttctgatag
     5941 aacaggcgga acagtgaaaa tgaatggtaa aagttatcct cttctagcaa aacagccact
     6001 tgtcttaaca agctattcaa aaataaatgc ataaaacaaa aaactgcttg cgacatatca
     6061 gaagacttta aatcttcttc tgatattgtc caagcaattt ttattttgaa gtttgcttaa
     6121 ttctgttctt tagaaacaat gtttgttctt tttccggcaa aaagaaagct agataatacg
     6181 acaagtaaaa agaaagcgat gattccaact gtcaaggcta tttgaccgta ttctcctagt
     6241 ttcccaagta caagtcctgg tactagataa tcagtatcgg caaatgtaga agaagcgatt
     6301 ttaagatctc ctaatactgg aaggataaat aatggcagcc aactaatcaa taagctattg
     6361 aaagcagaac ctaataccgc ccctcttcta ccgccagtcg agttgccgaa gacaccagaa
     6421 gcagctccgc agaagaaatg tcctactacc ccaggaatga taactgtagt ttgcaagcct
     6481 atcatagcaa acatggatag tgttcctaca aagaagctaa caaaaaagcc aattaataca
     6541 gcattaggtg catatggaaa aacgatagga acatctaatg ctggtttaga atcgggtacg
     6601 agttttttag caatcccttg gaatgctgga acgatttctg ccaacaccat tcgaacccct
     6661 tgaaggacga taacaaaccc agctgtgaat gttccagctt ggatcagtgc aaagattaca
     6721 gcgttgtctc cattgcttag ttctctttca acaaatccag gaccagctaa caaagcaagt
     6781 gcgacatata cgatcgacat caagagcatg atcgatacag tagaatctcg caaaaagctg
     6841 aaacttttag gaatctttat gtcctctgta gaacggtctt tgtttccaaa aaactttccg
     6901 atccatccac tagaagcata accgatattt ccggtatgcc ctaacgcaac ttgattgttt
     6961 ccagtgatgt tcttcataaa cggttgacaa agagcaggcg taatagttaa tagtgttcct
     7021 tcgaagaccc cgcctaataa gatcatccac aaatttgcag taaaccccgc gcttactaaa
     7081 atgactgctg ttagacaaga aacataaagc attgcttgac ctgtaagaaa aatgaattta
     7141 tatcttgtga atctagcaag taaaatattc acgatcatcc ctactagcat gatcaaagca
     7201 gtaggtgtac cgtattgttg caaagcaatc gccactactg cttcattgct aggaactact
     7261 ccttgagtat gcagagctgt ttggaacatc gaagcaaaag gggttagtga attactgatt
     7321 aaaccagcac cgccgatcag tactaggaat ccgacaaatg ttttgatagt tccttggatc
     7381 acttctgaaa atttcttctt ctgaagaacg agaccaagca gtgcaatcaa agaaacgagg
     7441 atggagggct gactgacgat tgacacaaac aaattcaaca atttatccat tgatagttcc
     7501 tcccttgaat catttttatt cttttgacaa gatcttcgtt actttttctt ttacttcttc
     7561 gctgtcaatg atcgattgca aaggtacgag cttttcatca ggtatagaag ttagcgtatc
     7621 tttcaaagaa tgctcaatga agaaataatc agccatatcg ctagatacac ttccaacatc
     7681 cgtatgactt acttcaaact cgtccacatc tgctccaaga tctgcaagtg cttgtttgat
     7741 gttcatctct accataaaac ttgttcctaa tccactttga catactgcaa aaattttcat
     7801 atcattcgtc tccttttctt atgatcgcta atatttcttc ttttgttgat gcgtgtaata
     7861 aatcttgaag attttgttta ttagaaagta gctttgttaa atttgccaaa gcccgaagat
     7921 gtgcctcatt atcgatagca gctaaacaga tgaacaaact aatgggatgg gctggattat
     7981 caagcagatc cacaggttgt tgaagtttca gcaaagacat ccctacttcg tttacgccat
     8041 cttctggacg agcatgaggt aatgcgatgt gatcgccaat atggataaac gcaccatact
     8101 gctccacgcg ctcgatcata gctttgatat aactttcttc tattttattt tctgataata
     8161 gcggctgtgc ggcaaatgtg attgcttctt gccagttttt tggttgatcc gataattgga
     8221 tcatttcttc tgttaataat tcagatagca taggacgttg atcctccttc ttttctaaaa
     8281 gtttgttcat tttacgattt aatacttgat agagcttttc ttcgtctacg ccttctttta
     8341 attcgatata aggcagtagc gccgacaaca attcttgggc agaaggcatt gaaaatcctg
     8401 gtatcagcca atctctttgt acttgattga tcagctgatt tttttccaag tcagtcatga
     8461 ttggatgcag aacatagaga gactttttct tatcagtaag tgtgagaggt acagtagaaa
     8521 aaataatatc gtaagaagtt tcgtcaacct cgtgtaagcg gtcagctgaa ttagtctctg
     8581 taaaaaagat catcgggaat aattgctgaa gttcactttt taagatcaat gaagagctaa
     8641 tgccacttgg acagacgatc acggctctta tttttctatt tctttcttct gcgtccgctt
     8701 ttctgatctc tccgccaaat agtatagtga aaaaaccaat ctcttcttca gggatgctct
     8761 tccctattcg tctttctaac ggaagtaatg cttttcttgt catttcgtag atggaagcat
     8821 attgatgctt aatttgatcg atcatcacat taggtaaata aaaagaatag gaaatacgaa
     8881 aataggcagg gaccaagtga taaaagacat tcatcaataa ttcacgaaac gaagcaaatt
     8941 ctacggccat caggaattcc atgcgatgga tgacttctga agaacattcc agcaaaaaat
     9001 caagtgcagg ctcctggaca tctccttcaa cgatcgatgc taagcaaaga gagaaaaata
     9061 taatctccga attttgatgt tcggaatagc caaggccagt caatacttgt cgactaatgt
     9121 cagtgactgc aagagaagcc aacagttcag catcttttac cagttccatg gatgtttttc
     9181 caattcgaat ggttgtcaaa ctcaaataat aagctaattc atccattcga tttggtgcga
     9241 atcgaagatc tgatagtgaa atcgttttat ctagcaaatc tctaactttg gcgtaataat
     9301 caatggaaag ggttgcggtt aaatacgtga gcccaaatct tccagcaagt gactgcatca
     9361 aacgagcagt gcgatttttt gcttctcttc ttaatattgc ttctgaccct agcagcctaa
     9421 aaccagtttt cctagtatac gacagctgac aatcttccac ttctaattca tttcttatct
     9481 ttttgatatc tgacaggact gtccctttag aaacctgaag aaaatcttga aaatgaaaaa
     9541 cagaaacaga cgttgcagtc gaataaacca tcaaataaag aagatctctt cgctcttgtt
     9601 cggtatacac agcttcaaat cgagtaatat ccaaaaattt ttctgcccag tcgattgctc
     9661 ggaatttctt tatacggata cgggtatcat ccacatattc ggcaaaagga gttaattgtt
     9721 tgatcgcctc gcttgtattt acttcgatga tctctgctgc tttttctaga ctgatttctc
     9781 ttggtgtttt caggagataa ctaattttgt agttcacagc gctgttcacc tcctacttat
     9841 attctagtat ggtagcgctt ttaaatcgag gggtgtttgg tacaacttac agttggattt
     9901 tttttacgac tgtaccgaac gatttctatc gaatggatac agaaaaaaag caggtgacca
     9961 aatgtctcct gcttttatga attgatttcg tccgatcaat gatgatcatg tttatttttt
    10021 gaggtcagtt cttcaaattt ttctttgaca tcttcaaccg ctttttcgat atctttttca
    10081 aagtgattgg gctcattagg ctttgtttcc tcttctacag aatttgaacc agataaagct
    10141 gtttctttat tttctgtttc accagagttt gtgccagcta atgattcttc tttgtcttct
    10201 gtttcaccag aattagttcc tgctaaagcc acttcttctt ctgttttagg ggccattgaa
    10261 cctcttaatc gtttgtgttt ttcaccgtca taatttgtca tgttggttcc tcctagtagt
    10321 tctatacttt ttcatcgtac aataagacag tttatgcgtc aaaaaacaaa cccgattctt
    10381 acaaatatgt catatttttc cttctgattc ttgaatgctt ttgattcctt cttccaaaat
    10441 tttcatttgt aagattgttt cttcatcttt gatgactttt tctgcgccat taactaatgt
    10501 ctcgtaaatc ccttcgtaga cacggctgta atcaccaacc tcggaaatta ctttttcttc
    10561 atgataactc ccttgttcat ctatataggt caatacacca taatgttcgg gcaaatcgat
    10621 accaaagtct tcatgatcag gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt
    10681 tgtttgtttc acgaacatcc ctttttttcc gtaaacgacg aaacttggac gttctttgat
    10741 gcggaaataa ctagatttta tcgaaatttt catatttcca tagaaaaaat ccagatcgaa
    10801 gtaatcattc attcgtcctt tccctaaaag ttgacgtaca tcatagctga tatcatctgg
    10861 ttcgccgaaa taagaaagaa cttgatctac tgtatgacaa gcatgtccat ataaatagct
    10921 gttcgcagta gaaaattcat gcgtattttc tggtacttcc ggtctgaaat aatcataatg
    10981 catttctact tctaaaatat cccctaattt tccgctttcg attacttttt ggactgtcaa
    11041 gaagtcagaa tcgaaccgtc tgttttggta acattggata aatagacctt tttcttttgc
    11101 taatgcaaat aactcttctg cttcctttga cgtttctgca aaaggtttct ctactagtac
    11161 attcttacca tgttcgagtg ccatttttcc atattcataa tgaaattgac taggtgtagt
    11221 gatgacaact aattggatct caggatcatc ccaaagatca gacagctgat cagtatattc
    11281 gatgtcttcg aatgttttcc acactggttc agatggggaa aaaatttttt tgatattgat
    11341 tttgttcgta agttttcttg aaaaaggcaa atgatagcga tttgtactct ttccattgcc
    11401 aagataagcg attgtaagca tgagtggtgc ctccttttat tgatgttttc agtatatcct
    11461 agaaaagcgc agaaagtatt tataatagct attctggtaa aaaagttcag aaaaacactt
    11521 gttatcattc ggtttggtga aattaatgtt caaagtaagg ataaaaatca aaaagatagt
    11581 agcggaacca agtgtttatc agtaagatag aatttggagg tcagatcatg cttattgaag
    11641 aaaaactaac aaaacaagag ttgtttacca cgacagagaa acggattgtc gattacatcc
    11701 gtaggaatat cgaagctgca gtctacatga cgattgaaga attagcaaaa gctacgtata
    11761 cttcccattc agctatcatt cgtttatgca aaaaaatggg attcagcgga tttaaagaat
    11821 tccgtttcga gctggggcgg gaagttcatc aattgattgc tcagttcaat caaacgattg
    11881 atcctaattt tcctttttca agtgaagata gcactcagca aattgcaaag aaaatggcgg
    11941 atttatcgat ccagtcaata aaaaaagcac aattgcagat tgaacatgaa gatttagatc
    12001 aaatcggtaa agtattgaac aaggcacagc gaatctttct ttttgcaaaa ggtgattctc
    12061 aaatcacggc aaggaaattc cagaataaaa tggtcaagtt gaataagttt ttgatcatgg
    12121 ctgaagaata cagtgattct tcttggaatg cagccaattt gactagcgag gattgcgcca
    12181 tttttattag ctacagcggt cgcatccatc attatgaacg aataatgacc tatttgaagc
    12241 atgtagaggc ccctaccttg ctgattaccg gaaatcagca ttcagaaatg gccaaacagg
    12301 ctgatatgtg tcttgttata tcgcaggagg aatatgattt tgctaaagta gcgactttct
    12361 cctctcaaat tgccttcgat tacgtattaa atacgttatt ctcagtgata tattctcaaa
    12421 attttgaaga gaatatccgc aacttgaaaa acaaacaaga attgatgcaa aaaggccttc
    12481 ttaaagaata tcggaaaact taagaaaaga tcctcattta gtaataagaa agatagtgtt
    12541 tgataggctc tctttgatat catcataaaa acaggctgaa gttttctttt tcaaacctca
    12601 gcctgtttgt ttacggaacg attcgaatag atatattata gaaatgagga atagctaact
    12661 tctgtttctt attttgaacg attgacgaaa aagatatata agaaggagga aaatcagtat
    12721 acatgcagct aacaatccat acaggaaaaa tcgtttgttc tcggatactt tctgtacttc
    12781 tttttgaact tcttttgcat gataaggaac ccggtgccct ctgacaagta agcgatgact
    12841 gttgaccata taaggtgtac aagttaggag tgtcacgaaa tcctgccctt tttcgattct
    12901 cagatctttt gtatcagtcg gttctaccgt cttgatctga tcgacttgat aagcgagtgt
    12961 ccgtccattg atttcgatat agaaagaatc acctaaagtc agtttcggta agtctgtgaa
    13021 caactttgct tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat tttctccccc
    13081 cgtaggataa gacgttcctt ccaataatga ggcacctttt tctagcagca cagtattcgt
    13141 ttcatcaaaa attggcaaat ggacattgat ctcagggatc gtcaaaatac cgatcgtatg
    13201 tttctcaaaa taggttctat cctttttcgc aggttctttt ttcttagtaa agggatctgc
    13261 acctggattt ccaccttctt ttgctaattg ctggtttttc tttgtcatgt tttcttggat
    13321 tttagccatg acttcttcat tttctttgac cgcttgttgc tgataatgag agatgatctg
    13381 ctgatccaga taattattca gcgcgtcgct aacaaatgga taagctagta tccctattcc
    13441 tgagagaagc aagaaaagca tcagcagatc tagaagtttc cttttctttg gtttcatttg
    13501 tgttctcctt gtgatgactg tttgataata aaagaaaaag gagcatttgc gtaatccgct
    13561 gctccttttt ccaattcttg caaaaagact aaatctgact gtgcttgcgt ctagcaaagt
    13621 acagtccagc aatcagtaga aggactactc ctgcaccgat atacacatag attcccttac
    13681 cgcctgttga aggaagtgta cctttgtgtt tatttggtat tttttcagga gaaatcaact
    13741 gtcctgctgt tacataagat tgttcatcga tagtaaatgc tacacggttt gttaatggaa
    13801 catatttttc tggcgctttc gtttcttcca gatagtacgt gccatatttt agacctgtca
    13861 catcgattaa accgttactt gtggttgtaa aaaccgttgc tgattctttc gccgataccc
    13921 agctgacggc ttttgtggat gggtcgatcg ataaatattt cgctgtgtca ctatcttgat
    13981 cacgaacgac gaattctgct ccagcaagtg tttggtctga tgtaacgtca ccatctactt
    14041 taacaaatcg tttgccccca gtaacgacat cgactgacgg tggtgtttga tcatttgtat
    14101 gcccgttatc gacatttgct tggttagaaa atcctttggt tggatctgct ttttcgttca
    14161 aatgcatata gtaaacgaat ttcaatgtac cgccaggagt taatgaagga atataattcg
    14221 gatcaactga aaccgtgaat ccgtctgttt gctcagtgac agaataattt actgggtcga
    14281 tttctttatt tccatcatat aaggcataag catacgttcc tgaagaatca ttatcaaatg
    14341 ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt gtacttgttt tgcgttcctt
    14401 ctttatcagc gatacctaat gggatattga cagaaatctc atattgaatt ttttcaccga
    14461 ttgcgacatc ttttccattc aattgaggtg ttgttttatc aactttagac gtatcatttt
    14521 tgatggtctt ttctactggt gtttggctat ttgccaggat ctcaaaaggc gtgattgttt
    14581 gcttttcaat catttccgca ttatcaggag cttttacttc ttctaaatta tattttccaa
    14641 cttctaaatg attaacgatc aactggcctt tttcaataga tgcctcggca aagtcattgt
    14701 tgccgatgtc ataagaatga ccagtaatga aatgtttggc tttggtttga tcagttgtcc
    14761 aagtgtacaa tccatctgtg acactttgga tgtatttttt gacgcttggt gttccttctt
    14821 ctttagaaat aataaattct gctccattta gtgcttcgtt ttcggcagta ccgatttttg
    14881 taactttcaa cgttccatca ttaccgactg tatttttagg gtagagatgg atagtatcta
    14941 gttcttctgt cccgtattta taagagccat ctgcttgttt gatcatctca tatacaggga
    15001 aagctaaaac catgtttgcg gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt
    15061 agactgcatc tttcccattt tgttttttag gtaaagataa agtgacattt ccatcagcat
    15121 ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga ctggactgct tgttttgctg
    15181 catccacgga cgctccttta tctcgttgcg cataaaattc ttgagtgacg ttataaactg
    15241 aaaatgaaat atcggctaat ccttggtatt gatcgaattc gctcatttct ttcccgctgt
    15301 tttggattaa aggatcgggt aaatcagtca ttttcttttt atgaaggatg acttgagctg
    15361 ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa
    15421 ggaaaaggac tcctaacata acgtttattt ttttatgatt tttcattttt tcactcctgt
    15481 tctttttttg ataaacaacc agattgcaaa gcttgcgaat accataccaa caacgcctaa
    15541 aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt cttttttatt
    15601 gactactttc aatatactgt ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa
    15661 cggaatttct gtttcactta aaatataacc agaaggtgct tcgatctctt ttaatcgata
    15721 acttcctgtt ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag
    15781 cgcatctgat tcgttctttg tccacttgta tccgttggct gtttgctgga ggtaatttcc
    15841 tgctttgttt ttaataagga atactgcgcc agctaatcgt ttatctggtt tatttgtctc
    15901 gattttgaca aattgcttac tccctgtata tacagagact ttctttgtac tgacaccaaa
    15961 accaaaatcc agtgttccgt tgttgttgat ttccttgttc gcctgtgctg tactgcttag
    16021 ctgcatctga taagaaatgg tcagtttttt tccagcatat ttttccaacg aagggatcga
    16081 aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca aagccttctg tcagcttttc
    16141 tccattcgat gaaatcgtta aactattagg taaaaacgtt aatgcttcat ccgcactatc
    16201 tgacaattcg aacttttgat agtcaaggat atttacagga attgtcgtat gcaaggaata
    16261 agtgattttt tcaccaatcg taaaatcatt tctaggctct tctaatacct tctcaaaagg
    16321 tggatcaggg tagtcgtttt cttcattttt aggatacaaa tggatagttg aaagtttttg
    16381 attgttttgt ccatgtacag gaagaacaac taccatattc tctgcctttt ctttgacgac
    16441 ttctggtact ttggattcaa tgaaaagata cgctttatct cttttttctt gatctttagc
    16501 ggccagagaa aaagaagcga taccatcttc gttgttaaga gttgtagttg ttgcttctgc
    16561 ggtaaacata ccggaagacg caccgttttt tgcgatctct gcttgtgctt cttctaccgt
    16621 tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac
    16681 accatttaat cctcgatacg tttgtaataa agctttttct tgtccgtcat ttggatgatt
    16741 tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt
    16801 ttctgtttct gttgctacct gagtaaaagc tggtttaaat agtaacaaga agagacacat
    16861 actaagccaa ccaagttttt tcatatttag ctgccccctc tttctttttt caaaaaaaga
    16921 taccaaatag aaaacgcaca accaatcatc cctactagat agattcctaa acggccaatt
    16981 cctcccgttt caggtaatgg tacttttgcc tgattcgtga tgtctaaaga aatctggttg
    17041 tttttggctc ctggtgacag aacagattca tgatcttgtc catccacttg aattgaccca
    17101 tcttcgtgta taactatagt aactggctct tttagacctt ggtatccttc tggtgtaaaa
    17161 gtttcggtca gcgtataagt tccaggtttt aaattttcga atagaaaggt atctgtttct
    17221 tgtccatctt ttggcgattc taagtccatt tctggtccct gcagtctgaa ttttgctcct
    17281 tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga
    17341 ttttgatgag tgagcgtcca tacattattt gtcatctcgg tattttttcc cgtgtaaatg
    17401 atttttccat atttatcgat ttggaattca taatttcccg gcgaagtatc atatccggca
    17461 ggaccagctg tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa actggcaaga
    17521 cctttttcat tcgttgtttg gctgtcaatt ggctgataag taccatttgc ttcttttttc
    17581 tgtagggaaa atgctgctcc tttaagattt atctctttgt ccgtcccttg catcgcatac
    17641 ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca
    17701 tcggaagtag tgactgtttt tccatcaatg gttaccgtac catcagaagc gatcttgatt
    17761 tcccaagtag tctttttgct taatccatgc ccttctggag cttttgtttc tgtcagcgta
    17821 taggtcattt ctttttgcaa tttgacattt tctggaagag aataggttcc gtcgccatga
    17881 tctgttagta aagtatcaat agaatctcct gttaatttga aaacagcgcc aataagatcc
    17941 ttttcacctg tagaggaatt tttcgttatt ttcaagttta acggtgtgaa ttgtttagta
    18001 tttttccatg tcattgcatc tatttgttga gaatcgtatc ctggtacagg taattctttg
    18061 attgtttggt aactgaatgc ttgaccttga ttattgtatt gaggtagtga taatatctct
    18121 tgataacttt ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt
    18181 gttgtatctt tagctggttt aatgattcga atatatccgt ttttccaagc tgcagcattt
    18241 gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg gacgatcagc tagattgttg
    18301 tcaaattctt cccataactt tttgatgtga agactgactc cgggagcttt agcagatggt
    18361 ataccgaatt cagctaattc attggtatca atgtttggct ggaaagttgt cctgccgttc
    18421 atttgatacc agaaatttgg atggaagtcc tcattttctg tttggattct cacttggtaa
    18481 tggatttgga tttcttggtc ttttcctaaa tagatctgat tgctcttgat ggtatttcct
    18541 tctatggaaa tagatggagt gaccgttgta ggacttgtcc ccacactctt gactgaaagt
    18601 gtaccaggct gataaatgaa tggttctgcg attggatcat ttatttgtcc attgcttaca
    18661 gttgctgaga tctgtacagc ttttttggct aggtattcag agatatctgt tgcatgatca
    18721 gctgattcat agtaaagatc gcctttttca tctgatgaaa ccatttgtcg catacgagac
    18781 tctacttctg cttttgagag accagctgcc ggatcgcttt gaagttggat gccaagacca
    18841 tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc gataaacgta
    18901 ctgtcgattc ttctggatag attgttttgg tcaggtgcat catagattct tgagattaga
    18961 gaagtatttc ccggccgatc ttgcgtatta gaaaactgag ttccgtaata attgctgctt
    19021 gattgtgcgt gtacccgctg tactttatag gaaaatgttg gtacaccatc cgtcagcaaa
    19081 acgatcactt ttttatgtcc atttggaacg gatagcatgc ttcctgcatc tcttagtgct
    19141 ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa tggattttac ttgatttttc
    19201 actgaatcaa atgaacccat ctgtactgca ccgttactgt agctatatcc ttcgcttgag
    19261 tagccgacat atcccatatt gattttgtct gtgataccgc tatctgctaa agtatcgaca
    19321 aaacgatcga caccaatctt tacttcaccg atccgattat tgtcgttcat acttcctgac
    19381 cagtctacga ccaatacgag atcaagaggc gtgatatctt tttgtacatt tccacgagca
    19441 ttcaaataaa catcaaacaa tccgggtgta cttgtttcct ttgcatactt tcgtatcgca
    19501 tagtctgctt gattaccggt tcctccatat tcgatatacg aatgggtccg atcattagga
    19561 tctccattcc aagaatttat accatcccat tgatttgttc cgtttttatt gccttgatga
    19621 tcaagaacat ttgtatttcc tgtaggttgc caactggctt taggataagt gccctgatca
    19681 tctgttgtat attctgtagg aatcgtttga ttagatggca cactcacagc tggcaaggat
    19741 aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt cttttgatga ttcttcttct
    19801 atttcgtctg gctttggtac tgacacatct gtatctttct tattttctgt ctcatcaata
    19861 atgtcatatt ctttattatc gacgatcagc ttgtttgcct ggctgtggta aagtgctgga
    19921 tcgattggaa aactcagatc gatagtttgt ttagtgtttg atgagatttc caacgtaatt
    19981 ttcccgctat ttacctgata agcatagcca tctccttctt ttctttccac atcacttaag
    20041 taattttcga ctagaccaat ttctttttct atcttttcag cactggtgtt attcgcattt
    20101 agcttgatgt tcagtgcttt ttttccagac tgatccgaaa tgacttcagc tgaaattgtt
    20161 tctaaaactg ttggatcatc tgtagtgtct gctattgcag ataatggcac aaagtttgat
    20221 aaactcaata ttatcagcat gaaaataact ttcagtttct tccctgtggt cgtcattttt
    20281 cccctccttt ttcttttttt ctgttttgac gtgaatagtg aaaattatta aaataaacag
    20341 gcgttacata attttattta ttttaatatg ataattatta tacgaagggc gctaaaggga
    20401 atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt cgattaatga
    20461 gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt gttggaatta
    20521 aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa gtggatgact
    20581 aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag gtatatcgaa
    20641 gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa attggggtat
    20701 cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat gtcgcaaagt
    20761 tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac gattgagtat
    20821 tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa acaatgcaat
    20881 caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca gttaactagc
    20941 cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag ttactacgga
    21001 agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat ggtcaagaat
    21061 aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt ctttattatg
    21121 tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg gaaagctgga
    21181 gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt gaaaaatctc
    21241 ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt caatacgatt
    21301 ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg
    21361 tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa
    21421 ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac tcatttgacg
    21481 atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct atatgctgga
    21541 tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat ttattattta
    21601 tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta tacttctgat
    21661 aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat tgaagaatat
    21721 atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat tactgaagat
    21781 cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca acccattttg
    21841 aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa tgaagaagaa
    21901 ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa ccaccctgat
    21961 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
    22021 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
    22081 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
    22141 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
    22201 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
    22261 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
    22321 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
    22381 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
    22441 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
    22501 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
    22561 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
    22621 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
    22681 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
    22741 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
    22801 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
    22861 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
    22921 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
    22981 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
    23041 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
    23101 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
    23161 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
    23221 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
    23281 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
    23341 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
    23401 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
    23461 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
    23521 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
    23581 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
    23641 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
    23701 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
    23761 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
    23821 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
    23881 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
    23941 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
    24001 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
    24061 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
    24121 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
    24181 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
    24241 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
    24301 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
    24361 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
    24421 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
    24481 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
    24541 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
    24601 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
    24661 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
    24721 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
    24781 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
    24841 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
    24901 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
    24961 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
    25021 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
    25081 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
    25141 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
    25201 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
    25261 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
    25321 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
    25381 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
    25441 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
    25501 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
    25561 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
    25621 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
    25681 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
    25741 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
    25801 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
    25861 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
    25921 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
    25981 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
    26041 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
    26101 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
    26161 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
    26221 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
    26281 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
    26341 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
    26401 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
    26461 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
    26521 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
    26581 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
    26641 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
    26701 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
    26761 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
    26821 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
    26881 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
    26941 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
    27001 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
    27061 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
    27121 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
    27181 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
    27241 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
    27301 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
    27361 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
    27421 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
    27481 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
    27541 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
    27601 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
    27661 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
    27721 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
    27781 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgttactt
    27841 ataaatttat tctataggag acgattgttt tc
//
LOCUS       DAEGIQ010000029        27703 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000029 DAEGIQ010000000
VERSION     DAEGIQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.029"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            414..947
                     /locus_tag="KMT45_001113"
     CDS             414..947
                     /locus_tag="KMT45_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBE7004586.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="KMT45_001114"
     CDS             complement(1437..1961)
                     /locus_tag="KMT45_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7004587.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="KMT45_001115"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="KMT45_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBE7004588.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="KMT45_001116"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="KMT45_001116"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBE7004589.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="KMT45_001117"
     CDS             complement(3683..4363)
                     /locus_tag="KMT45_001117"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBE7004590.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="KMT45_001118"
     CDS             4489..5358
                     /locus_tag="KMT45_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBE7004591.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="KMT45_001119"
     CDS             5384..5899
                     /locus_tag="KMT45_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004592.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6539)
                     /locus_tag="KMT45_001120"
     CDS             complement(5946..6539)
                     /locus_tag="KMT45_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7004593.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="KMT45_001121"
     CDS             complement(6564..6743)
                     /locus_tag="KMT45_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004594.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="KMT45_001122"
     CDS             complement(6820..7329)
                     /locus_tag="KMT45_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004595.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="KMT45_001123"
     CDS             complement(7425..7805)
                     /locus_tag="KMT45_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE7004596.1"
                     /translation="MNLTTIHPIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8135..8884
                     /locus_tag="KMT45_001124"
     CDS             8135..8884
                     /locus_tag="KMT45_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBE7004597.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9068..10345)
                     /locus_tag="KMT45_001125"
     CDS             complement(9068..10345)
                     /locus_tag="KMT45_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004598.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="KMT45_001126"
     CDS             complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="KMT45_001126"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBE7004599.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11267..12211)
                     /locus_tag="KMT45_001127"
     CDS             complement(11267..12211)
                     /locus_tag="KMT45_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBE7004600.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12213..12899)
                     /locus_tag="KMT45_001128"
     CDS             complement(12213..12899)
                     /locus_tag="KMT45_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBE7004601.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13372..13692
                     /locus_tag="KMT45_001129"
     CDS             13372..13692
                     /locus_tag="KMT45_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBE7004602.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            13853..14758
                     /locus_tag="KMT45_001130"
     CDS             13853..14758
                     /locus_tag="KMT45_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7004603.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            14755..15528
                     /locus_tag="KMT45_001131"
     CDS             14755..15528
                     /locus_tag="KMT45_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBE7004604.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(15705..17129)
                     /locus_tag="KMT45_001132"
     CDS             complement(15705..17129)
                     /locus_tag="KMT45_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7004605.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17340..17786
                     /gene="nrdI"
                     /locus_tag="KMT45_001133"
     CDS             17340..17786
                     /gene="nrdI"
                     /locus_tag="KMT45_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBE7004606.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(17856..19838)
                     /locus_tag="KMT45_001134"
     CDS             complement(17856..19838)
                     /locus_tag="KMT45_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE7004607.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(19852..20748)
                     /gene="murQ"
                     /locus_tag="KMT45_001135"
     CDS             complement(19852..20748)
                     /gene="murQ"
                     /locus_tag="KMT45_001135"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBE7004608.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(20751..21812)
                     /locus_tag="KMT45_001136"
     CDS             complement(20751..21812)
                     /locus_tag="KMT45_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBE7004609.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(22024..22590)
                     /locus_tag="KMT45_001137"
     CDS             complement(22024..22590)
                     /locus_tag="KMT45_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBE7004610.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
     gene            complement(22618..24120)
                     /gene="eat(A)"
                     /locus_tag="KMT45_001138"
     CDS             complement(22618..24120)
                     /gene="eat(A)"
                     /locus_tag="KMT45_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBE7004611.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(24378..25499)
                     /locus_tag="KMT45_001139"
     CDS             complement(24378..25499)
                     /locus_tag="KMT45_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBE7004612.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            25625..26566
                     /locus_tag="KMT45_001140"
     CDS             25625..26566
                     /locus_tag="KMT45_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBE7004613.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(26709..27605)
                     /locus_tag="KMT45_001141"
     CDS             complement(26709..27605)
                     /locus_tag="KMT45_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBE7004614.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
BASE COUNT         8024 a         5833 c         4929 g         8917 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta taggatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     7981 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     8041 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     8101 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     8161 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg
     8221 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     8281 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     8341 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     8401 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     8461 agaatggaat cataatatgg cttcaaaaat ccgaaaatta actggaaaaa gctggtttat
     8521 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     8581 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     8641 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     8701 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     8761 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     8821 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     8881 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     8941 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     9001 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     9061 ctatttttta tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt
     9121 cttctaactt ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt
     9181 ttcctttcca tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta
     9241 gttgccattt ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc
     9301 gttctttacg caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga
     9361 acccttctgt aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt
     9421 ggatgaatcg ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt
     9481 gacgttttgt ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg
     9541 gcacttcttc atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca
     9601 agaattcttc tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta
     9661 tctgtcgctt ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca
     9721 atgcttgata atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct
     9781 gagctaattt tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa
     9841 aacagtattt ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata
     9901 attcgtctaa ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg
     9961 gagagacatg caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt
    10021 ttggcgattt aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt
    10081 gctggcttcg ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg
    10141 ttgcagctaa ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat
    10201 cgactagttc ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg
    10261 tgtattgttt agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt
    10321 caaagacgga ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg
    10381 ctactaacat aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg
    10441 catttccgga aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag
    10501 aagcaatgtc ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg
    10561 gtgagaatgg cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat
    10621 gaagcaaacg atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg
    10681 ctggtagacc atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt
    10741 aagcggctgg accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag
    10801 ggattcttgc ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac
    10861 ctgcttgtcc gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta
    10921 cttgactgct tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga
    10981 tttcatctgg catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg
    11041 gatcaaaacc tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata
    11101 tggttatctt ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta
    11161 aataacataa cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg
    11221 ttagaagagg ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa
    11281 tcatataagc aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca
    11341 ctccgtattt gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca
    11401 tcggtgtcag cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg
    11461 gattcgctcc atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg
    11521 ccgcaaatgc attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga
    11581 tgccgatgtt cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc
    11641 caactccagc gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta
    11701 acggccctac tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat
    11761 ctcgagaaaa gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta
    11821 aagcacctaa attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg
    11881 gaataaaaat tttcattcct agtttgtctg acattttttc catatattct tttgatggaa
    11941 gccggctttc tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca
    12001 agccaccgtt cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg
    12061 tccagaaaaa ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc
    12121 cagtgtaaat caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta
    12181 ataaaatatc aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc
    12241 ttcgctaatt tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata
    12301 gccactggaa cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc
    12361 agtgttgatt ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac
    12421 cagcagacat tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg
    12481 acttctaacc cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca
    12541 aatccagcaa ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca
    12601 taaatatcta caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct
    12661 gattctttca gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc
    12721 gccatcccgc gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca
    12781 agaccgccag tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat
    12841 ttaaatgcaa acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata
    12901 tattttcctc cataaaatga caatattttt ttaattataa agcaaaacac ataaaaaata
    12961 tagttgtttt ctcacgaaat agacactatt tttaaatatt tttcgttatt tttttattat
    13021 ataattcacc ttacttattg taagataaag ctttttttaa tttattgcct gctaatatat
    13081 aaaaataagg tctagttact gtttttttaa atgattaata aataacaact cctttctatg
    13141 aaattgcaaa gcattcatct tcttttctgc taacgatata tccgtcaatg cttgttacac
    13201 tttactaaaa agtatgagat tgccgatatc tggataggtg tgctatacta cagaaaaaga
    13261 tatacgatct gccaccgggt agtaaaaaag aggatgatca ttccattagg tctttgaagg
    13321 gatgattctc ctctttttgt gtatttttta tattgaagga ggaacgtatg tatggcatgg
    13381 atggaattaa tcatagcagg aattttggaa gtattctggt caacgatgat gaaatggagt
    13441 gatggttttt ctaaaatcaa ttattctttt tacacagtga ttggaatgat tgctagtttt
    13501 tactttttgt caaaggcaat caaaagtttg cctatgagtc tctcttatcc tatttggaca
    13561 gggattggcg cagttggttc ggtcattatt ggtgtggtct tgtttcatga taagctgaat
    13621 ctttccactt ggttctttgt cgggttgctg ctcatcagca tcattggcat caaaatcact
    13681 agcggtcact agatacttag acaagcagac aaggactgat cagaaaaagc ggggcttttt
    13741 atttgatatt cctctttcta tgaaaaaaca agaaaataag actaatgacc tagcgacttc
    13801 tctactcatc aggtgtatgc ttgtactatc tatagaaaag gagtgaatat ttttgaaaag
    13861 tctttcggtc caagaattat ccaagaactt tggtcacaaa caagccctca gcgatgtcag
    13921 ttttagctta tctcctggca aaatcgttgg attagtaggt cctaatggcg ctggaaaaac
    13981 aacgatcatg aaagctattt taggactgat ccattactca gaaggaacga tccgtattgg
    14041 tgaaaatgag atttctccct ctagtcataa aggattagaa caagtaggag cattgatcga
    14101 atatccgggt atctatccat tcttaacagg ttatgatcat ctgaagttat tctctgaaac
    14161 aaacgatgta tctgctatcg acactatcgt gaaacagctc aagatggaaa aatacattca
    14221 caaaaaagcg aaatcctact cgctgggaat gaaacaaaaa ttgggaattg ctcttgcctt
    14281 actgaaccat ccgtctatcg ttattttaga tgagccgatg aacggtttgg atccacaagc
    14341 aacaagagat gtgcgaaaaa tcattaccga cttggctact cagggcacct ctttcttgat
    14401 ctccagtcac ttactcagcg agcttgaaaa aatggtcgat gagctgatcc tgatcaatca
    14461 aggaaaaatc atcaaacaat gtacaatggc tgaattgaac caaacagcgc aagatttcat
    14521 cgttatctct acgacaaatg atcaagctgc tcgaacgatc ttgacagacg ctggatatcc
    14581 cttggaagat cctgataaaa tcaagcttct aaaaaaaaac gaagagttat taggaactgt
    14641 tatccagtta ttgtctgcag aaaatgtcca tgtgacagat gtccagcatc tgacgaatga
    14701 tttggaatct tccctattgg aaattctgga ttcagtaaag ggggaagaag aaaaatgacg
    14761 ttgcttataa aacaagaatt atttaaattg atcaagaaaa agtcaactgc tgttttatct
    14821 gttctattag ttgctttgct cataggaaca gcacttctag ccaaaaaata cacgacgatc
    14881 attgatccag ttgaaatgac tgctcagctt ttcagtgcca catcttggat tgtctttatc
    14941 atgattgctg ctgcaagtac gatcatttca atggaatcac aatatggtac attgaaaaat
    15001 ctcctttatc gaaagtattc tcgaggagaa atattagtaa gtaaatggat cacattagtt
    15061 atttattcta tttatttata ccttttagct attattgtca ccgtgctgat gaaactgatc
    15121 cttttccctt cgatttcctt tacggagaag atttctactg gtcagacact gatccaaagc
    15181 ttattcactt atactttggg tagttatatc ggtttatggc taattttaag tttagtgttg
    15241 atgattgctt gttttatcaa tagttccggt gcctccatct cagcagggat cgtcttttat
    15301 tttgcttcat caatcatatc tggtatcctc atcgcactca ttcaaaaatg ggagtggatc
    15361 aaatggaatc caatcagtat gctgaatctg caaaaccaaa ttggcaacga agagattatg
    15421 aagcaattga ctcatctttc tactaatcaa atgctgtttg gcaatttagc ttacattgct
    15481 ttgttcttag cattgggata cctcgtattc aagcgaaaaa atgtatgatg gttcattaac
    15541 aaagaagttg tgacagaagt ggccagtttc aagaaatttc ggcttatttt tagaagaagc
    15601 tacttcttga acaccattta ttcaaaaaaa gaagcgatga cacgaatgtt gtcaccgctt
    15661 cttttttgaa taaaccacag gcttcacagc ctctctttat acttctatac gtgaagcatc
    15721 ccagtggcaa ataaaaccat tgcgattact ccgaccatcg tcacaccgat aagtaaaatc
    15781 ttatctaagt ggatatgttg tacaggttcg ttttgttctt tacgtgcttt gaggtaaaaa
    15841 taaatacctg gtaagaaaag aacaagaaca agcatcactt cattaaaggc tacaactagc
    15901 atggcccata cttgaaaagc acttgctaaa aaccctacta aaaattggac ccactctttc
    15961 cgctgataag aaagcttcat ttggtacagc cctaagaaaa gccacgtgaa gaaaatcgct
    16021 gctgtaccaa gtgtataagc aaagttatat gcactgtcag tgaataataa tgaaagtaaa
    16081 aagacattcg tacataacgt cgtcaatacg atagcataag ttggtgcccc atttttgttg
    16141 agttttcccc atgctttagg caaaagtccg tcctcagcca tattccccat tgtttcaatc
    16201 ggcaagattg tccatgagag ccaaacccca actaaagaaa tgatcaaacc gccgttgatc
    16261 acgaccgccc cccatttacc aaccatcatt tcaaatacat atcccatagc aggttgcgct
    16321 ttgatcgttt gcagctgatt catcgtcatt gtgccatatg gaagcatgga aaccaagacg
    16381 taaataatga tcaaaccgaa tattcctaga atcgttgctt tcccaacatc cgattgtttt
    16441 tttgctcgtg aacctacgac agaagctcct tcaataccaa cgaataccca gagcatcacc
    16501 atgatgcaac tcaatacttg actagaaaac gggatcggct gcccattcgc attcaatatg
    16561 atatcgctag aaaagttatt aatgaattgt tggatcgaaa aggtgctgat agctactaca
    16621 ataaataaaa ataatggcac taatttacaa aatgtgacga tagcattgac aaagctagca
    16681 ttttcaactc cacgattcac tagccatgca tatccccata agaagatcgt actcatcacg
    16741 atcgatgcca cattttgacc accttcgaat acggggaaga aataacctaa tgcactcatc
    16801 aacaatgttg cgaacgccac gtttcctaac cagcatgtga gccagtaacc ccaaccgctg
    16861 ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac taaagatccc gccttttaaa
    16921 tctggacgtt ttttattgat attattgatc gataaggaga gcatcaacat tgctgttcct
    16981 acgagtaccc agctgaagat agctgggcca ccagccgatc cggagatgtc cgtcataata
    17041 ccaaatacgc cacctccaat cgctgaacca atcactaagg cagcaagtgg aatcaaacca
    17101 attccttttt tctgttgctc tgtatccatg taagcatcct ccaaagtaaa accgctttta
    17161 tcaaaaagta taacaaacct tttttatttt tacttaatta ataaaaactt taatagaaat
    17221 ataattctac cgactttttt ctcattatac aagcaaagcg cctagtccct ttccaaatta
    17281 caccaccaaa aaaacttaaa aagatgctat actaaaggga aagaattggg ggatgatcca
    17341 tgaacatttt atatatatcg atttcaggaa atacgcgtgc ttttgccaaa catctagcag
    17401 aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa
    17461 tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt ttttactttc gttcctactt
    17521 atttagacgg cgggaatggg ttagacaacg gtgacacaga aatcttgacc gaaacgatgc
    17581 gcgaatatct ggaatacgaa gacaatcata aactttgcct tggtgtagtc ggtagcggta
    17641 ataaaaactt caacaatcaa tattgtctga cagccaaaca atacgcacaa agattcggct
    17701 ttcctttctt ggcagattat gaattacggg gaacaccatc tgatgtgaca cgcgtttatc
    17761 aaattttggc agaaaaaaat aaataacaca aaagaagaag ctgaaaaata aatttcggct
    17821 tcttcttttt atgttctcta ttgtttctat tcttattaag ctttgacatc catcacaggt
    17881 gcttttgctt tcgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg
    17941 gttaccaaga cgatcatgga ggtttctttg tctgctgctt tgattgcttc taagtcagct
    18001 tctgctacaa gcgtatcagg tgtcacttgc tgtccttctg ttactttcac atcgaatggc
    18061 gtaccgccta agtcaactgt gttgatgccc atgtgaagca agatttctaa gccatttggt
    18121 gtcgtaatgc cgatagcatg ttttgttggg aaaacagaac ttattactcc agtaaccggg
    18181 gaatagatat gtccgttttc tggttctaca aagtaacctt cacccatcat tttttgtgca
    18241 aaaactgggt cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgataag
    18301 atagtcacac tttcagctga agcttgaggt actgcttttt caggtacttc atttactgtg
    18361 gctgtttcgg tcgctagttc ttctttttct aaacggtctt taggaatacc gaagaagaat
    18421 gtagcaacga agccaccagc gtaagcagcc aataaaccaa ggatatagcc taaccattta
    18481 ccatcagaga tcaatggaat caaagcggct cctgaaggtc caatagcgat tgctccgaca
    18541 tttccgatca ttccgatcac tgccccaccg atacctccac cgatacaagc agtaataaat
    18601 ggacgtccta gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taagattccg
    18661 actggtaaag cccctttgat catttctgtt aattctttgt cttttttgca gcggatccat
    18721 aatgccaatg ctgcacccac ttgtcctgct ccggccatcg caaggattgg aagcaataag
    18781 gtacgaccag tttcatcaat catttgtaca tggattggcg tcaagatctg atgtagtccg
    18841 aacatgacca ttggcaagaa taatgtacct agaacgaagc cggcaaatgc tcctccaaca
    18901 ccaagtaccc agttgatccc accgaccatg ccgtcagaaa tgaaccctgc taaaggcatg
    18961 atgaagaaaa tttcgatcag accgatggca atcagggaaa gagttggcgt aacgatgata
    19021 tctaccgcat caggtactac tttatgcagt tttttctcaa cgattgacaa taaccaaact
    19081 gcgaagataa cacctaagat cccgccttgt ccagttgcaa gagcttcacc ggtaaacaag
    19141 tttttcagtg gtgcttctgg attcatccct gtcagcatca caacagcccc gataactcca
    19201 ccaagcgttg gcgttgcacc aaaaacttgt gcagcattga tacctgtata gataactaga
    19261 tagctaaaca tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttcct
    19321 gcgtctagtg ttccagctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc
    19381 ccagtgccga caaatgcagg gatcattggt acaaagatat tagagatgtc ttttaatacc
    19441 gatttgattt tagagggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt
    19501 tcgtttacac ggtcttttcc agatgcagaa gaacttgctg tagttgcact agcagaagga
    19561 agtttttctc ctaatttcac gcctgctaag tcaaccattt cctgcgctac tttgttcact
    19621 ttcccaggtc cgatgatgac ttggagctgt tcatcctcta ctacgccaag tactcctgga
    19681 atcgctttta attccgagac atttacaaga tcatcattga tgaccttcat acgtacacga
    19741 gtcatgcagt ggacaatgct ttcaacattg cccataccgc ctgctccttg ataaatttgt
    19801 tttgccagtt cttccaaagt caattcgttt tttgccatgg tcttttcccc ctcataaggt
    19861 tccttttaca aaaccgtttg cttcagctaa ttttgcttga gctgcctctt tatcactatt
    19921 tgtcaaaatc atgacgatag ccagtttgac attttgatct gcttcctcaa agtacttttc
    19981 agctgtttcg tagtcagctt ctgtcgcttg catgatgatt cgttttgagc gttcaattag
    20041 tttttcattt gttggtttga cgtcgaccat caagttattg aatactttac cgataccgat
    20101 catactgata gtagaaatca tattcaaaat cagtttttgt gctgttcctg acttcaatcg
    20161 agtagagcct gtcaaaaatt ctggtcctgc atcgacttcg attgggaaat ccgcatgctc
    20221 gctgatttct gcttgtttat tgcaagaaat cgttcctgta acagcaccaa tcgattttgc
    20281 gtaatctaat ccaccaatca catatggtgt acgaccgcta gctgcgatac caataactat
    20341 atcatttttt gttaaattca gcttgattag atcttcttgc cctaattcat aagaatcttc
    20401 ggctccttcg acggctacag tcattgcttc ttctccacca gcaattaggc ctatgaccat
    20461 ttccggttct acaccgaatg tcggtacaca ttcagcagca tctaatacac caagtctacc
    20521 actcgttcca gctcccatat aaatcaagcg accacctttt ttaaaggatt caatcgtgtt
    20581 ggcgatcact ggctcgatac ttggcagttc tttttcaaca gcagcggcca ctttctgatc
    20641 ttctttgttc atgagttgga ctgcttgaga aatactcatt tcatctaatc caaaagtttc
    20701 ttggtttctg cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct
    20761 tcttttctta taaattgata gttcatacca gctttgattt ggttgatcag cggtagttct
    20821 tctttgatga ctttggctac tacattcact ttttcatccg ctggcaaatc atatttagtt
    20881 aattggatct cacccatata gcgcaaatac ttttcgttat ctaaagtaat agatcctttc
    20941 atacgcttag ctgtatttct tactgggatt ggcggaatct ctcgaaaacg cgcatctgcg
    21001 cttcgtatga catctcttgc atcatcttgc cgattcgtat gttttcctaa tatatattcg
    21061 taaaaaactt catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa
    21121 gcaaattgct cctgaacgtg ttctgacaga cctgcatctc cgatataaac aatatccgtg
    21181 ttacttgcta atagatcaag agcacctgcc agaggatgga tccctcgatg ttcttccaaa
    21241 gttggtaatc cttgatacag tggacctctc aactctgtat ctcctggtac aaagccttgt
    21301 acaaggaatc cttgagtttt gagccattga tttttttcct gataccattc tttgtccaac
    21361 cctgtttcaa gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagccgct
    21421 gcttctttca actcatcgat atcattaggc gtgatcgtgc tggcattcaa actgattttc
    21481 aattgatgtg accagtcggc gatctgctga ttagaaatat gataatccat tcttaaccct
    21541 gttacaccaa ttgctttcaa gggtgttaga tcttctaatg aaaagccagc tcgtttcaat
    21601 gcctctcctg aaatatcgac catcaactct agtttattct ttttcgcttc gcttcctagt
    21661 tcgatcaatc gattttgata agccgaaaca tcatcttccg gtatatgcat ggaagtgaaa
    21721 ataccgacaa agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa
    21781 gaatcattca taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt
    21841 catttctatg actagagtat atatctaaag aaacaaaatt tcaatacttt tgttaaaata
    21901 attaaatttt atttctaaaa attggattta taatgccaaa agaattgaca cagccacaaa
    21961 aaaggcactg ctaagcaaaa gcagtgcctt gaaacatttt atttaaataa cacaaaagtc
    22021 taattactca taagaaatta attctgtatt gtctaccatg aatgtaaaga acccttcatt
    22081 aggcattgga tgaaaataag cattgatcac tcttatcaag gcattatgac agactaacaa
    22141 gtagacattt tcttcgtctt ccatgcattc tttcaataaa ggaacgatcc gtgcgtacac
    22201 atctaagacc gactctccat ttgggaaacg tacagcaaac gctaaacgcg ctttttgaaa
    22261 gttttcatct ttgcttggca gtccgtcata atccccaaaa tccatttcta ccaatcgctc
    22321 atcagcggtc atcggcaaat tgagacgatc aactactgct tgtgctgtat ctcttgctcg
    22381 ttgaagaggg gaatggatga ttttcgtgat tggaacaggc aaatctacta ccttttctgc
    22441 tagtgcctgt gcctgcatct gtcctttttc agtcagcggc acatctgctc gcccgcaaac
    22501 taatccttgt gcgttccaag ttgtttctcc gtgtctcgtt acataaagca tgtgattctc
    22561 tccttcgttg acaaagttct ccttaaccat accttgttgt ctttgagatt acaaggttta
    22621 gattttctca agagaaataa tctcagtaga tactttatct agaaaggttt gatcatgttc
    22681 cactagtaac atagcaggtt tcacgttcaa aatcagttgt tctaattgtt cttgattgaa
    22741 gacatccaaa taattcaatg gttcatccca tatatagagc tcagctggtt gggataaaga
    22801 tttagccaat tccacttttt tccgttgtcc catactcatc tgttcgattt tattatgaaa
    22861 aacatctctt tccatcccaa gctttcggag attattcaaa aatgcttgat agtctactcg
    22921 gtttttcttc gcgaattccg ccaatgtccc tcgattgtct tcataatttt ggcgtacgta
    22981 actaatggtc aaatgtttgg gctgggattt ttctcctaca accttgccgt tgaatgtccc
    23041 cagaagataa tggataatgg atgactttcc cgcgccgtta ggacctgaaa ttgccatccg
    23101 ctgatgaggt tcgatcgtaa aatgaactgg ctcgaataac agattttcat accctaattg
    23161 aagattttct acgcttaaca gccgtttatg atgagacact tggctattca tcgtcaaagc
    23221 atcgatatac tcgatatctt ttaataattt ttctttctca ctgatctggg tttccatccg
    23281 attcacgatt gctttagatc gtttcatcgt ccgtgcagca tcagcgccta ctgctccttt
    23341 attcactcgc ctagattccg tatcgatgaa tccgactttt ttctttgttt tgtccccttc
    23401 tcgggaacga gaccattctg ctttttcagc tgctgttttc tttagcctgc tgacttcttt
    23461 tttcaatttt tcatttttag ctatttcaaa ttcatcacga agttttttct gttcttcata
    23521 gatagagaaa ttcccttgat aaagttccag ttggctcttt tcgattgcta aaacatggtc
    23581 cactacttca tcgataaatc cccgatcatg actcaccacg atgaatcctt gttttttctt
    23641 tttcaagtaa gccgctactt gttttctgcc agagatatcc aaatgattcg ttggttcatc
    23701 gattaacggg aaatgagtgt catccacaaa taagagtgcc agtaggactt ttgtcttctc
    23761 tcctcctgat agtgtgctga attctctcca taagatttca ggatctgttt gcatcagttg
    23821 gagctctctt tcaatttccc atatctcaaa atccataatc tcatttaaca cataataggt
    23881 caaacgctct ttatcttttg tttgctgcgg gaaatagaca aacgcttgct gatggatcac
    23941 ttgcccttga taagggagtt tgttttgcag gatattcagt aaagttgtct ttcctcgacc
    24001 gtttcgtccg ataagtccta gtttccattg tgtgtcaaag tttagattgg cttgttcaaa
    24061 taataatgtg ccttggctgt catagccgaa tgtcagattt tttatttcga ttttagacat
    24121 tattccatca cctcgtaaaa aaagaccagt cctattttca gaactggcta aagaggatgc
    24181 aagggttggg gcacacaaaa agacccatac atcgaaagaa ccaagccaat cccgaaaatc
    24241 tgcacactgc tcccttgtct ccacaaggta cagtctgaat gcagctttca tcaatcgact
    24301 taatttttca atgcacggat ccctcacttt gtcctatatt tgaaattacg atagcataaa
    24361 gaaattgttt ttgtcaacta cttcgggaga atgttgaaaa atctgctgag tgtctgtcct
    24421 aagatttctg ttttttctaa tgtttgcctg tgttccattg cttcctctaa atcgattggt
    24481 cctaattgaa tgcttaagac aatggggaga aaatcaaaga gtccttccag ctctcggtct
    24541 ctacttccgc aaatagcgat tattggtttt cgatattttt ttgcaagcac tgctacgcca
    24601 tatggaactt ttccgtggat tgtttgctga tcaatccgcc cttctcctgt aatgatgaca
    24661 tccgcttgtt cgatctgttt ttctaatcca gtgatttggc tgaccaactc aaaaccagag
    24721 accatcttcg ctcctaacaa aaaaagggct ccgccaatac cgcctgctgc tccgcttcct
    24781 gggatgtcat ctaaatccac ctcccaaaca tcttttatct gctgagcaat cttttcagct
    24841 tgtttatcca agaaagctaa cgtcgtatcc gttcctcctt tttgtcttcc aaattgccaa
    24901 gcagctcctt gttgacctgt atatgggttg gtcacgtctg cagcgatgat cacttttact
    24961 ttagaaacaa gaacgttcct tcgatccaaa tgaagttttc tagtagataa caaaggattt
    25021 ccttctgtgt acccttctaa agaagcaccc aaagattgga gtaatcctag accaccatcg
    25081 ctacatccgc ttcctcctaa agtgacaatg atttgttcca cttcttgaag cagtgtatct
    25141 ttgataagct ctcccaaggc ataactagaa gcaagtcgga tggtttgatc cgttggttta
    25201 tcaagtaaat ctaatcctaa tacttgtgca gattcaatga ctgcggtatt ttttccttgc
    25261 cagttcgtca ataaataact gatttttcgc tgacgaaata agagatccat cccatcaatc
    25321 gtacgccagg ttcccccgag cgcgtaatag attgcttctg cagtattttc tccgccatct
    25381 gcaatcggca cattgatttt tcttccttta cggtcttccc aaccatttag aactgcctga
    25441 tttaattcaa atgaagaagc acttccttta aatgaatcga ttgccgccaa aatatccatc
    25501 tatatccctc actttcttct taagtaagac actttttttc acttttttca aacaaaacaa
    25561 tgctattttc ttctgttttt ctatatacta aaagggaact cattgaataa aggagagaaa
    25621 aaacatgaaa atcgcaatag ctggagccgg agcaatgggc agccgaatcg gattaatgtt
    25681 gcatcaaagc ggaaatgagg tcgtcttgat cgatcgctgg cctgctcaca ttgaagccat
    25741 tcgtacaaat ggattgattg ctgactttaa tggcaaagaa gtagttgcaa aactgccaat
    25801 ctattcacca gaagaaatca tcgaaagcaa caaacacgtg gatttgatcg tagccttgac
    25861 aaaagcgaat caattagatg atatgtttcg ttcgatccaa tccattatta ctgagaatac
    25921 ttatgttttg tgtttactga atggattagg tcatgaagat gtactggaaa aatatgtacc
    25981 aaagaaaaat atcttatttg ggatcacgat gtggacagct ggtctagctg gtcccggaaa
    26041 agtcacatta ttaggtgatg gagaaatcga actagagaac ttggaaccag aaggcgaagc
    26101 atttacaaaa aaagttgtag aagtctttca agaagcaaaa ttgaatccaa tctatagtca
    26161 taatgttcgt tattccattt ggcgaaaagc ttgtgtaaac ggaacattaa atggattatg
    26221 cacgatattg gattgtaata ttgctgaatt aggtgcgcaa aaagcggcag agggcatggt
    26281 tcgaacgatt gtatctgagt ttgcttctat cgcagcaaaa gaaggaatca cattagatca
    26341 agaagaagtg taccaacata ttgcttctac ttatgatcct gacaacatcg ggttgcatta
    26401 cccatctatg taccaagacc tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa
    26461 tggtgccgtt tggcgaaaag gacaaaaata tgatattgct acaccatatt gtgccttttt
    26521 gacccaatta gtccatgcaa aagaaggaat ccttggcgca gaataaaagg ctctctgatg
    26581 agaatcagct ggatcagcag ctggttctca tttttttacc cccatgaaag ttcaaaaaga
    26641 aaaacacaat tctcatgttg ccataagaat tgcgcttgtt tattttcatc tatgattggt
    26701 tggcactttt atttattttt aacagaggaa agatcgtatt catattgttc ataaatagcg
    26761 gagtcagtag tgaaaaaacg ttttgcccaa ttttctgcat caaaaaattc caacagttct
    26821 gatgggattg gttgaaatgg cgtatgcaaa aattccggta attcatctaa atcacgttct
    26881 tgaagcaaaa aaatattttc agaatgataa aactgataat gagctatggc ttgattatct
    26941 gtcactagtt tcttttgaaa atacatagat tcaaacgttc gcaaagtcac tccttgctgc
    27001 ccttcccgca acagttctag aatccccctc gattccccgg tttttttcag atattcttca
    27061 taagtaaggt atccaggttg ttcatttcct cgaagcggaa gaataaagta attggtcgta
    27121 acacctcttt ttgtaaattc ctgcttatat ttttctgccc gttcttttct gccgttattt
    27181 gttgcaccaa aaaatagatc cgtctttggt tcgctagaag aaaaatccat atgtttcgaa
    27241 taaaaggaac tattatgttt tagtccaagt tctttcgctt ctaaaaaatc aaaatgataa
    27301 atctcatcga ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg ttctgcttta
    27361 aagtggttcc aaaaaaataa cacgattttt gcattggtaa atggacgcac gcgtcgagcc
    27421 atctccatat caaatccata atctaaaaaa acgatcagct gataatttgc caactgttgt
    27481 cgagctgatt taatgaattc cgactgtagt aaatcttttc cggaccaaac atctaaagag
    27541 cggatggaag gaaaaaaata aagtgattta tttttcgcaa ttagcaatac gtcgtttttg
    27601 ttcatctgtt ccgcctcact ttctctgtca ctttttccta tgatagcctg tttaaatagt
    27661 aaaaagccag taaaacgctt cctattattt ttataaaaat tta
//
LOCUS       DAEGIQ010000030        25656 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000030 DAEGIQ010000000
VERSION     DAEGIQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.030"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            81..2141
                     /locus_tag="KMT45_001142"
     CDS             81..2141
                     /locus_tag="KMT45_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBE7004615.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2224..2793
                     /locus_tag="KMT45_001143"
     CDS             2224..2793
                     /locus_tag="KMT45_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7004616.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2968..3774)
                     /locus_tag="KMT45_001144"
     CDS             complement(2968..3774)
                     /locus_tag="KMT45_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004617.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3912..5426
                     /locus_tag="KMT45_001145"
     CDS             3912..5426
                     /locus_tag="KMT45_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBE7004618.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5829..6812)
                     /locus_tag="KMT45_001146"
     CDS             complement(5829..6812)
                     /locus_tag="KMT45_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBE7004619.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6817..7464)
                     /locus_tag="KMT45_001147"
     CDS             complement(6817..7464)
                     /locus_tag="KMT45_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE7004620.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7549..8181)
                     /locus_tag="KMT45_001148"
     CDS             complement(7549..8181)
                     /locus_tag="KMT45_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBE7004621.1"
                     /translation="MKNTKNFTLTAMLLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8197..8748)
                     /gene="coaC"
                     /locus_tag="KMT45_001149"
     CDS             complement(8197..8748)
                     /gene="coaC"
                     /locus_tag="KMT45_001149"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBE7004622.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8745..9482)
                     /locus_tag="KMT45_001150"
     CDS             complement(8745..9482)
                     /locus_tag="KMT45_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBE7004623.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9479..10468)
                     /locus_tag="KMT45_001151"
     CDS             complement(9479..10468)
                     /locus_tag="KMT45_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE7004624.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10726..11268
                     /locus_tag="KMT45_001152"
     CDS             10726..11268
                     /locus_tag="KMT45_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7004625.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11285..12370
                     /locus_tag="KMT45_001153"
     CDS             11285..12370
                     /locus_tag="KMT45_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004626.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12382..13188
                     /locus_tag="KMT45_001154"
     CDS             12382..13188
                     /locus_tag="KMT45_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004627.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13189..14016
                     /locus_tag="KMT45_001155"
     CDS             13189..14016
                     /locus_tag="KMT45_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004628.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14013..15086
                     /locus_tag="KMT45_001156"
     CDS             14013..15086
                     /locus_tag="KMT45_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE7004629.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15514..18210
                     /locus_tag="KMT45_001157"
     CDS             15514..18210
                     /locus_tag="KMT45_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBE7004630.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18287..18775
                     /locus_tag="KMT45_001158"
     CDS             18287..18775
                     /locus_tag="KMT45_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE7004631.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18787..19281
                     /locus_tag="KMT45_001159"
     CDS             18787..19281
                     /locus_tag="KMT45_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7004632.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19302..20111
                     /locus_tag="KMT45_001160"
     CDS             19302..20111
                     /locus_tag="KMT45_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7004633.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20108..20929
                     /locus_tag="KMT45_001161"
     CDS             20108..20929
                     /locus_tag="KMT45_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE7004634.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            20980..23079
                     /locus_tag="KMT45_001162"
     CDS             20980..23079
                     /locus_tag="KMT45_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBE7004635.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23076..24194
                     /locus_tag="KMT45_001163"
     CDS             23076..24194
                     /locus_tag="KMT45_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBE7004636.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24233..25111
                     /locus_tag="KMT45_001164"
     CDS             24233..25111
                     /locus_tag="KMT45_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBE7004637.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25302..25454
                     /locus_tag="KMT45_001165"
     CDS             25302..25454
                     /locus_tag="KMT45_001165"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBE7004638.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8108 a         4460 c         5427 g         7661 t
ORIGIN      
        1 tgattttatt tatataatga aatcatctta tgaaaattac tacttcatgt ttagacaaga
       61 gtagggagga ctaagaataa atgtgttcaa aaagacgttc aaaacaacta aataaacacg
      121 cttgtcgttt agataaggaa cggtcgtgtt ctgaaaaaca ctacgttaag tttatgatgc
      181 gcacgatgct gatcagttta tctgtaggtg tctatatgca tgtacccgtt caggcaacga
      241 cacaaaccat caaggtcgta cgtacctctg tttctgagac cactgattcc cctatcacta
      301 ttactcatac tttcactgat gcaaatggaa aacagctact tcctgcattg acttcctata
      361 ataaagtcag ttatcctcca acgataaacc actactggtt aattcctaac aaaacattat
      421 ggacatcgga aaccggaagc agtgtgccgc tttctatctt ttccgatatg actgatccta
      481 caaaaggtga tcagttaaaa cagatcactg atattttgaa tcatgctttg acagatataa
      541 aggacggtgg aagcgcacaa gtttcttatg tatatgaaaa agattattca cagtttgaaa
      601 gaaagaaaat cgatgttctg gcagggacaa aagtttcgat atctgatctg gtagaaaggc
      661 tgaagaacgc agatggaaca caaggagata tgtccaaagt aatttcaaac aatgggaata
      721 tcgtggatac gactaataca ggaacatcta ctatttcctt gtcctatttt gatccaattg
      781 ctttaaaaac aatgactgca tctgctgtga tccatgttcg tgctgatcaa acagctatca
      841 aaggaaaaga tcttcactta agaacaggag agagttacgc ccctaaagat cttgttgaat
      901 atgtaacaaa aagcgatgga acttcaggaa ataaagaaga aatcaaaatg aacgggacag
      961 tagatactac aactccggga gaatatcccg ttgtattaac ttatatagac tcgaaaacat
     1021 caaatgtggc agaaacaaca gctgtagttt atgtagaagc atctgctgct tcatcgactg
     1081 tcatcactca ttttatagat agtcaaacaa ataaagaact tcaagcagaa acacatatga
     1141 gtagtgtaaa tcgtattcct gatttgcgcc tacctgctga attttctgat cgagtgccaa
     1201 atatagattt atcgacttat gaacaaaatg ggaaagtcac ttctatttct gattggatgc
     1261 aagcaaacgg attagacaga aaaagtcact ggtcggagat ccttgaagta cttcactcag
     1321 aactgatgaa taataggaag caatcaggag gggaaattac ctttacttat gtttatatac
     1381 cagacaatcg ttcattagaa ggtcatgatc ttattgttca tccaaacgaa aaaataaaca
     1441 aagaagagtt gatccaatca gcaacaaatt ccttaggtaa acccgtaccg ttaaaggatg
     1501 tatcggttac aatcaatgaa gtaccattgg aggatggtta tttgaccaaa caaacaggtg
     1561 attttacgat tatgtatagt tatcatgacc cgtattcact ccaagaactc catgctcagt
     1621 cactattaca tgttttacca ttaaacaaac agatagatca aaaagaacaa aaaagacaag
     1681 aagaaagctt gtcagaaaaa aaacaacaaa tggcccatgc agtttctcca acagggaaag
     1741 taaaacaaac aagaagccta gagttagcaa gaatatctag aaaaatctca gaatctaata
     1801 aaacgataag gcaagaagct catttggccc cacaagtttc tggattggaa aatgaaattt
     1861 tagagcctaa aataaaacaa caatcagaaa aagaatttgt tgctaaacag tcggaagatc
     1921 agaaggaatc agatgaccag aaggaaacga agaaaaaacg cttgtcaact attgattcca
     1981 ccgatgagca tctgcagccg caagtaccac ttcctagtcc gggaacaccc gcacctggtg
     2041 gaggtattcc ttcttctgct ttaggagatt tcatgcgagg tatggcagga acatgggtat
     2101 acgctaatcg aaatgaattt gacgcagatg actggttctg atttatatct ctatctgtta
     2161 tagtcaaata agtagctgag tcatatgtta tttggcataa tggagacgag gagggataga
     2221 aaaatgttag ataaaacgtt accttacgca gaaatctgga tgacaagacc acttcaacaa
     2281 aatgttccaa tcacgccttt agcggaaggt tttgaatttc gattttatca aaaagaagat
     2341 gaatctgctt gggcggagat tgagacttcc gttggggaat ttcaaacgac agatgaagca
     2401 cttgagtatt ttcaacaaaa ctttgctcct tatcctgaag aacttgaaag aaggatgttt
     2461 tttgtagaga caaaaaaagg agaaaaagta gcaacctgta ccgcttggcg aaaaaaatca
     2521 agagagcaag tgtatccggt ttttcattgg ttagcagtga aaccagatta ccaaggaaaa
     2581 gggctcgcaa aagcattgac tgccagagtc ttgcaagaat ttcctaccct ccatacggac
     2641 ggaccaatct atcttcacac acagacatgg agccatcagg cgattgctct ttataaaaaa
     2701 atgggtttta cttttattgc tgaaaatcta gacggcacac ctaatccaga ttatgaaaaa
     2761 gttatgcagg ttttaagtaa attagataaa taacgaaatg gtgaaataag tcctcagtat
     2821 tgggataaaa aacgaaatag tcattcctca tatcttactt ttttgagtta atactatcgg
     2881 ataactaatg gcatgccata cattcaaaaa agaaggggca gtgacaaaag tattgtcact
     2941 gccccttctc ttgtttttta gcatgtttta tcgtttgatt tttttaggaa taaaattctt
     3001 gatgaacgta aatttttggt aatcgtttcg actatagagg taaccaaata cactgaaaac
     3061 aactgctcca ataaaattaa caaataaatc cttcatagta tccatgattc ccacgtctaa
     3121 gtaaccattt tggatgattg ttttatctgt tttaccatct gcattttgac tttcaatgat
     3181 tgtccgagtg atatctggaa catgatagac agtgtttccg tctttactta actccacact
     3241 attgatttgc tggatgatcg tatctttttg catatcgaat tgcagtaatt tatctgctga
     3301 aaattccaca aactcccaca taacaccaac tgtcatcgaa aaacagaagg aaacaactgc
     3361 tagtaataaa ggagtcaagt tcatggtttt tgtattttta ttgagcaaat aaacaaggga
     3421 aaagccgaca cccccagcta aaaatccgct gaatgcatgt agaaaagtat cccacattga
     3481 aaaatagcca taaaaatcac ttaactctcc taaaactgtt gaactaaaaa taaaagataa
     3541 caaaatgatt tctagcatat ttggaatctg tatctttaat atcttttcca gaaaataagg
     3601 gatttcaaat aaaattaatg tcagtaataa aagcattgta tttccccagt gttggttcca
     3661 gacctgatcc actagcataa taacgacgat gatacgtaat aaaatataga ttccgtttgt
     3721 gattctacct gcagtttttg tactgttcgt aaaaaaggtt ttcaaatgat tcataaaata
     3781 actccttcat gtatatcctt aattatacta agaagcgtgt gtttaccaaa gctatatgac
     3841 ttaaagaaaa acagagttga tattgtgaaa aaaatcactt aatatagtat aaggagaaaa
     3901 gggggataca aatgaaaaaa ttagttgctg gtttattaac agccgttgca cttcttgcta
     3961 tgagcgcatg cggcgcagat cagaaaaata cagcgcagac aagttctagt acgaaggaat
     4021 ctgccgaagt atcatcaggc gcttctgaac aagtatatac agatccaaaa gaaatgaaag
     4081 aatcttatga tgtcgtgatt attggagctg gtggtgcagg gatggcagca gctatcgaag
     4141 caaaagatgc tgggttgaat ccagtaattt tggaaaagat gcctgttgcc ggaggaaaca
     4201 catctaaatc atcaagcggt atgaatgctt cagaaacaaa atttcaaaaa gaagaaggaa
     4261 tcaaagactc gaatgatgcc ttctatgaag aaacgctaaa aggtggtgga ggaacgaatg
     4321 ataaagaaat gcttcgatac tttgtcgatc actctgctga tgcggtcgat tggctagatc
     4381 aaaacggtat caaactggac aacttaacga tcactggagg gatgagtgaa aagaggacac
     4441 atcgtccgtc tgatggctcg gctattggtg gatacttagt ggaggggtta ttgaataacg
     4501 tccatgagcg ggaaatccct atttttgtca acgcagatgt gacagacatt aagaaaaatg
     4561 gggaaacggt caatgaagtg actgtacatg ttcaaggaga agaaccaaaa gaggtcaccg
     4621 gaaaagcaat cgtagttacc acaggcggtt ttggtgccag caaagaattt attgaaaaat
     4681 atcgtcccga tttagctgat tatattacga caaatcaaga aggatcaact ggagatggta
     4741 tcaccatgat tgaaaaatta ggtggacaaa ccgttgatat ggataaaata caaattcatc
     4801 caactgtgca gcaggatgag ggggtcttga ttggtgaggt tgttcgcgga gaaggagcaa
     4861 tcttggtcga tcaagagggg aaaagatttg tcaatgaaat ggatacaagg gataaagtat
     4921 ctgcagcaat tacagcattg cctgaaaaat cagcttattt gatttttgac caaggcgtgc
     4981 gtgatcgtgc cacagctatt gaattttatg accaaaaagg atatgtcaca actggaaaga
     5041 caatggaaga attagcagaa aaaatcgatt taccaaaaga aacactagca aaaacagttg
     5101 atggatggaa tacggatgtt gcggagaaaa aagatgctca attcaaccgt gccacagcca
     5161 tggaacatga cttatcgtct ccgaattatt atgcgatcaa aatcgcacca ggaatccatt
     5221 acacgatggg cggtgtgaaa atcaatacga acactgaagt attagataaa gaaaaccagc
     5281 caattagggg gttatatgct gctggtgaag tcacaggtgg cttacatgga aacaaccgga
     5341 tcggtggaaa ttcagttgcg gagatcatta tttttgggcg acaagcgggc atacaagcag
     5401 ctcgatttgc ttcggaaaat gaatgaaata gaacgtaaat aaaaagaggt tgtgaaatca
     5461 gcgttttgag ctaataccga gggtcagctg attgaacact gtttagtgaa gaggttgtga
     5521 aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
     5581 aacttggttg gacttattac cgcagatgca agcttttgaa caccgtttat tcggaaaaag
     5641 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     5701 aagcagaaga aattccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     5761 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct tttttgatca
     5821 gcatgatttt attcagttat cttcaaaatg atttttccga tgtttttgtt tgcttccata
     5881 tattcatgcg cttgtttgac tgcgctgaat tggaatgtac ggtcaatgat tggccgaatc
     5941 ttgccatttt ctagaaaagg cccagctttt tgcataaaag tttgtgcgag ctctgcttta
     6001 tactgatcac ttctaggtgt caataatgta ccaagcagtt ggacacgttt cccaatcaat
     6061 acaccaaggt ctatctcagg cacgatggtc ccacctagca taccaatcaa gacccaacgt
     6121 ccatcaacgg ctatagaagc taaattcttt tcccagtaag aagcgccgat gaaatccaaa
     6181 ataacatcga ctccccgacc ttcagtgatt tttcgtacct cttcttgaaa gtcttgtttt
     6241 ttatagttga tcaattcatc tgcaccaagt tctttacaaa aggcgagttt ttcttctgat
     6301 cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag ctaattggat cgctgctgtt
     6361 cccacaccac ttgctcctgc atggatcaag acagtttcgc cttcttgtaa ttttccaagc
     6421 cagtaaagtg tttgataagc agtcaaaaaa acttctgaga ttccagcagc agaaacatag
     6481 tctagcgtat cagggagaag gattgcacga tcggcaggca tcacagcata ttcggcataa
     6541 ccacctagat tgactaatcc gtatactttt gtcccaggag taaaagtgga gatatcactt
     6601 ttattctcaa ctacctctcc agctacttcg actcccaaaa tagggtaggg aggtttcaaa
     6661 gagactgatt ctcttttcat aatatctgtt cgattgacag ctgctgtatg gactttgatc
     6721 agtaattcgc cttgttttgg tttaggaaca ggcacttcaa tttgttctaa tacatctaca
     6781 gaaccaggat gcttgattga aatggcacgc atggatctat tcctcctttg cccattcttg
     6841 gaatgctgta ttcaactctt tgctaccttc agaaagtact agagtcgtac gagaaatatc
     6901 cgtttcttcc actagagcgg caagtacagc agcaactgta gagcgagtga tcgtttttga
     6961 tgccttctca ttaggcttag ttaattgaat ggaagtaact tcttcatcgt tagtcaatgt
     7021 gactggttga agaatgacaa aatctaagtt agttcgtttc agccattcat ctgcataatg
     7081 tttagtaata taataatctg tcatggactc aggccatttt gctggttcat ctgcataaac
     7141 agcactgacc atcagatagc gagagacatg cgcattttca gctgcgatca ctgttttgat
     7201 tgcgccatcc aagtctactt gcagaagatt ctttccttga gagccggcag caaaaatcaa
     7261 tacatcgatg cctttgaatg cttcggtcat tttttccacg gaccaagtta aatcaaaaga
     7321 gacatacgaa acattctctt tttttactaa tgtttgggaa gcaacatctc tcacccctgc
     7381 aaaaatttca tgggaacttg gagctaaatc ttttatcagg tgtcggccaa tttgtccgtt
     7441 tgcacctaca acaaaaattt tcatttggat catcctttcg acgtcaaatc tttccattca
     7501 atagtaacac caaaagagaa aaaaatcaga gatcggctac gatgactatc agttagtaag
     7561 agaagggtgc ttgaagtatc ttaacaagac attgcatact gctgcagtcg caagacctgc
     7621 gacgattcct tcaggtatac cacttgtaaa aataaccgct agaatagctg aataaacagc
     7681 atttaaattt gttccgatga ctttggcata atcttgctgg aataaaaagt agatcatatt
     7741 catcactaaa atcgtattgg tcaatgaacc ggaaagtcca gctaagaata aagaaacgga
     7801 atctgctttt tgtttcatta gtttgcgcag acctttataa acgaaataag gaaccacccc
     7861 aatcaagatg cgaggaataa acgcgatcag caaggctttc cagcttccat gttctgttcc
     7921 gatgactggg ataaacggag aaaagacaaa agacaaaggt gtttggataa ctgtgctacg
     7981 gatcatgctg atcagtccaa atgtaccgcc aagaaaagca cctagtcttg gtcctaaaac
     8041 gatcgaagca atgattacag gaatatgcat ggttgttgca ttgattggac cgatagggat
     8101 aaaacctaaa ggcgtaacag ctaataaaat caaaatagct aaaagcatcg cagttaatgt
     8161 aaagtttttt gtatttttca taagtactcc ttttttctaa gcatttcttt cattaagagt
     8221 cagcatgacc tgttcgatga tcgtctgaat atcagctaag gcaccttttc caaaatctcc
     8281 acaagctaaa agagattctc ttggttcaat ttcctgatag ccgacagatc tcaatgtttc
     8341 cagattacgc tgagtgatcg ggttttgata catattcgtg ttcatggcag gggcaatcaa
     8401 ttttggtgtt cccactggaa gcgccattgc gactgttgaa agaagatcgt ctgcaatgcc
     8461 gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg atgagaaaca agtcagcttg
     8521 ttttgctagt tcgatatgat tgattacctg cggatttgat tcttgcatga cgtctgtatg
     8581 gactgcgcgc ttcgacagag attgcaaggt taatggcgtg ataaaatgtg tgctgctttt
     8641 agacataatc acatctacct gggcacctaa tttgacgaat tgacttgtaa gatcagcgct
     8701 tttgtaagca gaaatacttc cagtaacgcc tagtaagatc tttttcattt tgattcctcc
     8761 agtctaattt tctcagtaat caatgcagca atctcagatt tactttgagc ttcttccact
     8821 gttccatctt tagataagag atagccgtgg tgtgtctcgc catggatttc cgtcaaatca
     8881 tttgcgaaaa taaaatcagt tttgttattt atcagactat tttttgcgac tgccagaaga
     8941 gattcctttg gtacatctac taatagtttg aaaccaatga tttttgcttt tggctgccat
     9001 tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa cgagcacaag atgctcggtg
     9061 ttagaagaga tctttgaaac agtggtatct gtctgttcat tttccgcaaa ccaattgtcc
     9121 agttcttctt gcgttgaaga agtgggtagg ttttttgcca gctgttcttc tgaaaaacta
     9181 aaagcaggag taaaatcact gactgccata ctgtggataa tcgcatcgta gtgttctttt
     9241 ttcgttagtt cttctaattg taaaaataag tccagtgcag attcgatcgt ataaatcgtt
     9301 atattggagt gtcttttcgg tttcagtgct tgtttcgtag tcacgtaatc gattgtggta
     9361 tccggattgg cagctaaata atcagctaat gcttgcccta atcgtcctgt cgaatgattt
     9421 gtaatagcac gtacttgatc gattttttca gtcgttcctc cagctgtgat tagaatcttc
     9481 atttatggtc acctacattt actagtgttc ttccttggtg tgttccagca agtagtttag
     9541 atgctgtttc aggcaattga tccagagtaa tctcttggac gaccagttca tctgcaatct
     9601 gcaaatccgc catctgttgc cataaagaaa gacgttgatc tattggtaca ttgacagaat
     9661 caattccaat caactggatg tttcgcagaa taaaaggcaa gacagtcgcg tttagtttga
     9721 ttcccccagc attgccacat aaagctactg caccgttata agagatcaat ggaagaagac
     9781 tgcttagctg ctcgccacct actgtgtcga tgacatagtc gatttgctgt tttcctaaag
     9841 cttttgttgt ctctggcaga aattcatcag gtcgtacgac ttgcaaagca cctagacttt
     9901 ttagccatgt gacagactct tcttttctgg aaaaggcaat gattgactgg aatcctagtt
     9961 tatgtaacag ggctatcgct gtactgccta caccgccaga agcaccagta acgacaactt
    10021 ttttatcgga ggtcatacct tgacggatta aagcgttcac acacaacata gcagtaaacc
    10081 ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt ttttggtaga ggaactaacc
    10141 agtctcctgg tactttttga tattggctat aaccgcctgg atgggtgacg ccaagcccaa
    10201 aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg attgttactt tggacgacag
    10261 ttccggataa atcaatccct ggagtcatcg gatatgttcg tatcacctga ccagactcgc
    10321 tcattgccaa tgcatcttta taattgacat cagaataggc aatttttacc aagacttcat
    10381 tttcagccaa ctcagtaaat ggcagatcac tgattgctgt atggaaaaca ggttccttta
    10441 ttacctgaaa agatcgaaaa gcgttcattc gtgagacact ccttttaaaa ttttctttta
    10501 tcataccata aagcctactc ttttattctt gttcttccgt aaatttagac aaaaattagg
    10561 tgaaaaataa agaatcaaac aactagaatt tattccaaaa gataaagaaa tattcagcaa
    10621 aaaaaatttt acaaagggtt gcctttttca ataaatttcg tatactaata cgagtggtag
    10681 taaacaggaa gtttactatt agaaaacttc gattcggagg aaattatgga aattggtgag
    10741 aagttaagaa atttgcgcat ccaaaagaat ttgacacaag aagaactagg cgaacgcaca
    10801 gatttgtcaa aagggtatat ttcccagttg gaacgagatt taagttcacc atcaatggaa
    10861 acattctttt ctattcttga agtcttaggt gtgactccag aagaattttt tcatcaagaa
    10921 gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata cggtttattt cgatgaagaa
    10981 aacgggtatg aattggaatg gctcgtggct gattcgaatg aaaaagaaat ggagcctgtc
    11041 ttgctgactt ttgaaggtgc aggagaatat aagacgtttg aaccttcatt atctgaaacg
    11101 tttatttttg tattagaagg ggaattagag ttaagcttag gagagacggt ctatgtggca
    11161 aaagcaggac agtcgatcta ttatcaagcg acaaaacaac atcaattaag gaatcatctc
    11221 aaaaaacgaa caaaagtatt gattgttgca acgcaatcat atttgtaata tagggggtaa
    11281 aaacgtgggg aaaccaatca tttcatttga tcaagtagtg aaacgctatg atgatgaaac
    11341 agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa ttttatacac tgcttggtcc
    11401 ttccggctgt ggtaaaacaa cgatcctacg tattatagct ggattcaccg aagctacaga
    11461 aggggacatc tattttgaag ggaaacggtt gaatgatttg ccagctaata agcgacaagt
    11521 aaatacagtc ttccaagatt atgcactttt tcctcatatg aatgtttttg ataacgttgc
    11581 ttttggattg aagatcaaga aaatgtccaa atcagatatc gaaaagaagg taaaagatgc
    11641 tttgcgtatg gtccagcttc ctggatacga aaaacgggag attagtgaaa tgtctggtgg
    11701 tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat gaaccgaaag tattgctatt
    11761 agatgaaccg ttatcggcgc ttgatttgaa actacgtaca gatatgcagt atgaactgcg
    11821 ggatctgcaa caacgattag gaatcacgtt tatttttgtt actcatgacc aagaagaagc
    11881 gttggcaatg agtgatgaga tttttgtcat gaataaaggg aaaatcgtcc aaagtggtac
    11941 acccgtagat atttatgatg agccaatcaa tcatttcgtt gctgattttg ttggggagag
    12001 caatatcgtg gacggtgtaa tgatcgaaga taatctagtt gagtttgttg gtaagcaatt
    12061 tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt gaagttgttt tacgcccaga
    12121 ggatttgacg atcacgacac cagataaagg aaaactagtc gtaacggtcg atactcagct
    12181 gtttcgtggt gtccattatg aaatcatctg ttatgatgaa cagcaaaatg aatggatggt
    12241 ccattcaacg aaaaaagcta aagaaggaag caaagtagga ttggcctttg agccagaaga
    12301 tatccatgtc atgcgtttca acgagtctga agaagaattc gatgcacgtc ttgacagcta
    12361 cgaagaatag gggggacaag aatgaaaaca atgcgcagga tctattccgt cccttatatt
    12421 ttgtggctcg gtttattcgt gatcgcgcct gtattaatga tcatttatca atcttttttt
    12481 gatatgaatg ggcaattcac gctgaataat tatctatctt attttacgtc ggggacatcg
    12541 ctttctatga cgatcaattc tgtatggtat gcatttttga tcacgttttt cacgctgatc
    12601 atcagttacc cgactgctta ttttttaaca aaactgaagc acaagcaatt atggctgatg
    12661 ctgattattt tgcctacatg ggtgaatctg ctgcttaaag cttacgcatt tattgggatt
    12721 ttcagtgtgc acggcagcgt gaatcatttc tttgatttta tcgggatcgg gccgaaacag
    12781 atattattca cagacttcag ttttctgttc gtggcaacgt atatcgagat tccatttatg
    12841 attatgccaa ttttcaatgc attggaagag atgaatcctt ctttgatcag tgctagtaga
    12901 gatttaggcg cgaatagctt tgagaccttc cgacgagtga tttttccgtt atcattaaat
    12961 ggtgtgaaaa gtggtgtcca agcagtattt attccttctc tttctttatt catgctgact
    13021 cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg ctatcgaaca gcatttcttg
    13081 gttacacaaa actgggggat gggttctaca atcggtgtga ttttaatcat tgctatgttc
    13141 atcgtgatgc tcctgacagg cgagaagaaa aaaggaggtc gctcataaat gaaaaaattt
    13201 aaatggtcgt atctttattt agggatcgtc tttttattct tatatgcacc gatcttctat
    13261 ctgatttttt attccttcaa tgaaggagga acgatgaaca gctttacagg atttacttgg
    13321 gaaaattatg aagctgtttt tgaagataca cgtttgatca ttatcgtatt gaatacgttc
    13381 atgctggctt tcttatctgc attgatcgcc acgatcatcg ggacatttgg ggctatggga
    13441 atctattaca cgaaaaaacg acgtgcacgg acagcgttgt tgagcatgaa taacatctta
    13501 ttagtctctc ctgatgttat tatcggtgct agtttcttga ttttctttac actactagga
    13561 ttcggattag gttttcagag tgtattgctt tcccatattg ctttcagcat tccaatcgtc
    13621 gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt caatggtcga tgcggctaga
    13681 gaccttggtg ctaataattt ccaagtagtc aaaaatatca ttttgcctta tctttctcca
    13741 ggaatcatag ctggatattt tatggcattt acctattctc ttgatgattt tgcggttaca
    13801 tttttcgtca caggaaatgg ctttacgacg ctctcagtag agatctattc tcgtgcaaga
    13861 caaggaatca gtttagaaat caatgcgcta agtgcgttag ttttcttgtt ctccatgata
    13921 ttggtcattg gatactactt tattagtaaa gaaaaccgtc caaaacggct gaagaaaaac
    13981 agaagagtac gcgagggggt ggcacggctg cgatgaaaaa attacaatct ctgtttttag
    14041 gtattctcat cattattgcg gtgctgctgc ttggtgttcg tcaacttgaa aaaacaagtg
    14101 gcatgagtgg agcaaaggta cttaccatct ataactgggg agattatatc gatcctagtt
    14161 tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa ttatgaaaca tttgattcta
    14221 atgaagcgat gttcacaaaa atccagcaag gtgggacaaa ctacgacatc gctataccaa
    14281 gtgaatatat gatccaaaaa atgatcaaag aaaaactagt acttccatta gatcattcga
    14341 agatcaaagg attaaataat attgatccac gatttttaga cttggatttt gatccagata
    14401 atcagtattc gattccatat ttttggggaa ctttagggat cgtatataac gataaattct
    14461 ttgatgcaaa tcaaattaag cattgggatg atttgtggaa gccggaatta aaagacagtt
    14521 tgatgctgat tgacggtgca cgagaagtta tggggctttc tttgaatagt ttaggctatt
    14581 ctttgaatag taagaatatg gcacagctga cagaagcagc aaataaattg aatcatttaa
    14641 caccaaatgt caaagcaatc gtggcagatg agatcaaaat gtatatgatc aatgaagaag
    14701 cctccgtggc agtgacattt tctggcgaag ctgccgatat gatgtgggaa aatgaacatt
    14761 tacattatgt gatcccacca gaaggatcca atctttggtt tgataatatc gttatgccaa
    14821 aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa ctttatgcta gaaccggaaa
    14881 atgcggcaca aaatgcagag tatattggtt attctacacc aaatgaagaa gcaaaagcgt
    14941 tattgccaaa agacatttcg tcggacgaac aattctatcc atctgatgac acgatctccc
    15001 atcttgaagt ctacaaagat ctaggatcga aatacctagg catctataat gacctattct
    15061 tagaattcaa gatgtatagg agataatggc aaaactcagt aagaatgggg acttatcaac
    15121 gttctaaaga gctaaaaatc gtcctttttc tatctgagtt ttccaatctt ctaccaaaag
    15181 cggatgaaac gccgcttttt ttgtgttatt ttattttttc taccacgttt ttctaccaga
    15241 gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg ggctagtaaa tctaagattg
    15301 aaaaagttac agaaggataa agtaattgga aactatgcaa atatacttaa cccttctgta
    15361 atctattaat aaaactaata tatgaatcca gttcaatatt tgtagctatg aaaaaacata
    15421 aaaatcatat tagataaaaa ctaaattggt agataaatta ttttgtcaga tatctgaggc
    15481 aaatattcga ctatttgtgt ttcaaagtaa aaattggaat acaaatatag aatagacata
    15541 ttgcagccga tagtttatta tgcttttaga cacattaaac tggaggaaaa aatgaaaaag
    15601 agcgctaaag atcgagtttt tgatgagctg atacgactaa cagggaatat gtcttctgtc
    15661 actgccgaga aaatttcgaa gaatctaggt atctctcgtc agaatgccag ccattatttg
    15721 acgagattag tggaagataa aaaggtcgag aaattaccgg gaaagcctgt tttgtggaag
    15781 cctttagatg aatatgcggt tgtagataat acggagcaaa taaacgaagc ctttcattct
    15841 gtagttggtc atgacggaag tctgagagag gtcattcaaa aatgtatagc cgctgtcaaa
    15901 tatccgccta acggactcag tgtattaata aatggagcca cgggtgttgg aaaaagtttt
    15961 ttagccacaa aaatttttga atacgctatc catgagcaaa ttattgaaaa agatgcacca
    16021 tttgctatct taaactgtgc cgattatgcg gacaatcccg aactgctttc ggcaacactg
    16081 tttggataca agaagggggc ctttacgggg gctgaaaagg ataccgatgg tttattggca
    16141 acggccaaca acggctatct ttttctcgat gaggtccatc gcctatcgaa agaaaatcaa
    16201 gaaaagctct ttctatttat tgatactgga aactatcgtc cagtcggtga aaatgtaaat
    16261 tggcattctg ctaaggttcg ttttattttc gccacgacgg aaaaaggtga aaattattta
    16321 ttagacacct ttgatcggcg tattcaaatt agtgttatgt tgccaacttt tgaagatcgc
    16381 cccattcgag aacgattaga gctgattcaa ttattttttc aaaatgaagc ggatgtttta
    16441 caaaaagata ttattgtttc aagagaagcc ttgtctttaa tgttatctca tccctttagt
    16501 gggaatattg gtaagctgaa gaatatcatt aaaatcagtt gtgcggatgt gtatagtcga
    16561 acacaagaag aaccattaac gattgggaaa aatgagatct tgattcagtt aaatatgctg
    16621 gaatccgaag tagaaagctt gccgattgct agtttattga taaagaaaaa tcaaaagata
    16681 aaaacggatc gaatcaaacc gtttgattat gattcgattt tggaaaaaat tactcagctg
    16741 ataaatgaag gtaatttcaa tgaaaatttt tcttcgataa agaatgaagt acagcatttg
    16801 gtgcatcaca ttgataagga ttctttttta tcaatgagta gaaaattgat gattcagatg
    16861 tttagaaagg tctgggtatc cgttttagga aaaacgtacg gattgacgac gagtggacct
    16921 gttgcagaaa ttattagcag atcctatgtc atttccccaa gctataagac agacttaacc
    16981 gggataaatc gtacattagc tgcaaatcta cctcggacga cttacttatg taatcagttc
    17041 ttggagcatt taccaccgct ctcagaaaag gacgagaact ttctaagact gcttattgtc
    17101 gtgactttat ctgattatgt agatgaatcc atcgagctga agggcctgtt gcttgctcac
    17161 ggagatagta cggctagcag cattcaagca gtagtgaatc aattatgcgg caattatgtt
    17221 tttgaagcgt tggatatgcc agtagagagt aatgtgtcag agattgtcga atcgacaagg
    17281 aatttcgtga aacggcaagt gaatacagat agtctagtgc tgatcgtaga tatgggctct
    17341 ttaaatcaaa tttacacaca gatcaagaac gatttaaaag gggagttact tcttctgaat
    17401 aatttgacga cttccattgc acttgatatt ggcttgaaga tgacgatgaa tgcgccattt
    17461 aaacagattg cggaggatgc agcagacaag tattcaatca atgttcaata ctttgaaggc
    17521 ttttctcaaa gtagcaacat tatcatttcc tgtatgtcgg gtttggggat ttcggacaaa
    17581 ttgaaagatg tttttcgtca tcatttatcg gatgaatcca tcgaagtttt cacgaaggac
    17641 tacagagaac tacgtgaatt aatcgaccaa aatgatgaaa cttattttga caagacaaaa
    17701 ttggtcatca cgacttcgga tcttccacgt tccttttcaa ttcctaatat taatgtttat
    17761 gacatcttag acggtgatgg agcagccaat ttggagcata ttttgagtcc tgaaatgaag
    17821 ccggcagaat ttaaccgctt gattcaggag ctagtaaaat tcttttcgtt ggaaggaatt
    17881 gcggaccgtt tgaactttct gaacccaacg atcgtgatga gtgaagtgga aacagtcatt
    17941 tctaaatatg aaaattacta tcatttcact ttaaatggcc gtgtaaaact aaatctctat
    18001 atgcatacgg cattgatgat ggaacgttta ttcttggctc ggacagaaaa atctgaagaa
    18061 ccgaattatg atttgaaacc agatgaagat gaattttatt atgtttctag aagtattttt
    18121 caatcaatgg agatgaaata taattttaaa gtcaatgctt atgagctttc gctgctctat
    18181 gaattattag gaccgtatat tcaaaaataa agtgtctaaa aaagtgtcta atctgttttt
    18241 tttaggttag acactttttt taatgtgaat taacgtgatt aaaccaatgt ttttaaaagt
    18301 tggcacagga attgcttata taaaagtgaa cagaaagaaa ggaattaacc atatgaagaa
    18361 aattctaata gcgactcacg gtcacttagc ttcaggcttt cttagcagta ttcaattact
    18421 gacggggaag gttcaagaga ttacggtgat taatgcttat gtggatgagt gtgattttga
    18481 acaggagttg aatgtatttg taaaccaggt gaacgagcag gatcagattt ttgtttttac
    18541 cgatttattt ggcggaagtg tcaatcagaa gattaccaaa acctttttgg agaaagagat
    18601 tgctgtaact attttgtctg gctttaatct gccaattctg ctcgaaatag tattggcaac
    18661 caaggaatta acaaaggagg atattcagga actggtaaat aaatgccaac aggaattaaa
    18721 gatgaccatt cttgatgagg tacatgtagt aaatgaagag gaagattttt tttaggagga
    18781 agaaaaatga ttaaagcagt gcgaattgat gaacgattaa ttcatggaca agtagcgatg
    18841 acttggacaa agagtctaaa tttaaccggg ttagtagtag caagcgatga ggcggctacc
    18901 aacgatattc aaaagatgac attgaagatg gctgcaccat cgaatgtcaa agtcattatt
    18961 aaaacagttg atgacgcgat tcatttgtta aaagatccgc gggcgaagga catgcgcttg
    19021 atggttttag tccctaatgt caaagatgcg gtaaaagttg cgaaagcttt cccaacggaa
    19081 atcgaattaa tgaacgtggg gaatgctggg aaaatgacac cgccatcgga agataagaaa
    19141 accttatcaa aggaagtgat gttaacgacg tcagaaattc aagacttaaa agaattagtt
    19201 gaactttatc cagataccgt ttttcagcct acaccggcga tggaaaaacg ttcagctatc
    19261 tctattttga gtgattttta gaaaaaggaa ggagaaaaat tatgtatgat gcatttgtag
    19321 tcgctttggc cgtttttatt ggagttgctg gacatgaaat gttcgggatg tccatgctga
    19381 gtcgtccgat tgttgttgct ccgttagcag gagcgttgat gggggatttg cagacggggt
    19441 tgattgtcgg ggcgtcacta gaagccatct ttatgggtgt cgtcaatatc ggaatctcta
    19501 gtacggcgga gccttctttg gctgctgggc tggctacagc gttcgcaatc aaaatgggag
    19561 gaaacgtgga tgcgattatt cccattgtct ttccactagc agtattagga ttacagttaa
    19621 tgaatatgat tttttctttt gtatgtggac cgatggctgc taaatttttc aaatttgcaa
    19681 aatctggtga tcaaaaagga atcatcaatc tgcacttctt tatgtggttt gtccactatg
    19741 cgctttatgc tcttattcca ttctttgccg ttttatttgg agcagatgtt gtcaaaagcg
    19801 tcttggataa tattccagag gtcatcatga acggattaac agtcgctgga aatctgctgc
    19861 cggcagttgg gatggcgatg ctgttacgga tgctttggag taaaaatatt gctgtgtggt
    19921 ttttctttgg ggccgttgtt atggcgtatc tcgagttacc attgattgca attgccgcag
    19981 ttggaacgat tattgcaatt gttatagccc aaagagatct acaactaaaa aatatatctg
    20041 ttcatgcagt tcaaagcgat gctgctgtaa atgcaacgct tgaccaagaa gaggaggatt
    20101 tctttcaatg aaattagctg atggaatttc taaagatgag aaaaaattgg ttcggaagat
    20161 gttttggcgc tcgatgacta tgtatgcttc ggtgaatccg gtgacgatgg gcggcggtgg
    20221 tttcgcctat tctatgatgc ccttcattaa tcatttctac aaaagtgaag aagatcgagc
    20281 tgcggcgctg gaacgtcata cacagtattt ttcgacgacc attccttgcg cgtcctttgt
    20341 catggggatt gcggcatcga tggagaagga aaattctgaa aaagctggtt ttgacgtgga
    20401 aagtatcaac gcgatcaaaa cgagtttgat gggaccattg gctggaattg gcgattcatt
    20461 gttctgggga gtttggcgtg tcgttaccgc gggactagcg atcaacttag ccaatgccgg
    20521 aaatattttg gcaccaatta tctttttagt tatgttcaat atacctaatt acttgatgcg
    20581 atactatggc ggcgttttag gctattcatt aggctctaaa tatattgaaa agctgtatgc
    20641 gaacgggtta attgaaattc tgacaaaagc tgctagtatc ttgggtctga tcatggtagg
    20701 tgcaatgacg atcagtatgg tcaaattcaa taccgttttg agcttttcaa tgaagggcga
    20761 agaactaatg aatgtccaag aaatcctcga tagtattttt aaaggaattg taccgttatt
    20821 ggtaacattg agctgtttca aattactaga taaaaaagtc ggcgtcatca aaattatttt
    20881 tggaatggtc gtcttgggaa ttttattgtc attacttgga atcgcatagt aggaagctgc
    20941 agctactagg ctgcagtttt tttagcaagg agggaaaaga tgactattaa tgaagtaatt
    21001 tattcaccca aacacccatt ttttaatgct gagttaaacg acggaaatgt gactacttca
    21061 aacgaataca gctactatcg gaatattcct gatgtaaagg gattgacctt cgatgtaagt
    21121 aatcaaaaga cattagcaaa ccttgatgtt tgcgggacga ttcaaacgtt gaccttttat
    21181 cgagaaaatt tactaactga ggaaaaacca ggtgtttggg tcaataaaca gctctctcaa
    21241 accaattcgt tatccttaaa ggttgaagta aacgggattg tctaccattt gtcagaatcg
    21301 gatcatcgta ttgaagtcga tgtgattcaa gactgtttgc ctagatatat tcatcattat
    21361 tcgacgttta aggtcattgt agtatccttt gctcccatac tggctaataa gcgtttaagt
    21421 atgcttgttc aacaggttta cattgtgaat gaaacacacg aaagccttca agtttcagtg
    21481 aaagatgtgc cttcgtatca agagaagtat tcggatcagc aaaatgtgtt gatttctcaa
    21541 aaaggaaata aggacacgat cgcacaacag gaagtggcaa gcttcgccat tgcttttgtg
    21601 gacccaaatg cttttgaaga agtaatgact tttcaggaat ctgatatcga aaaatggctg
    21661 aaggaaacat tgaactattt tgctcagcta tttgggcagc tgtcaatgcc aaacgataga
    21721 gttgtccatc tctatcgtcg tgcactttat caaagctttt ctagtttcgg tatgaatcga
    21781 cagggacagg tcgtcggcag caattggggc agctatcctg ctactaatcg aatttggaat
    21841 aaggatatgt attacgcatc attgccattc accatgtttg aaccggagtt atgtcaaaaa
    21901 acggttctat ggtttgatca atatggcgtt aagtttcctg gttcaaaatt tacaggaggt
    21961 atcaatcatt ctctgagtaa ttcgttatcc agtgcgttac tggctagtct ttattttgaa
    22021 catactgggg atacaaaatt ctttgagcgg tgtccgcagg ttctactgaa tgcttcaaga
    22081 attattgagg acatattagc tcaacgtcgg tcaggggaac caatgctttt tccatctgtt
    22141 tggttatcag acgcctttgc acttggaaag tatcataccg gcagcaatct ttgtctttgg
    22201 aaggcctgta ttggacttgc agaaattttt gaagccctag aacaactgag tcttagcaag
    22261 cgatacaaaa acattgcctg taaactaaag gaatcaattt gtaaacaaat gactatcgat
    22321 ggtccttttg gtgagcaatt tttggaggga atcggtgatg aggagaaaac aacctattca
    22381 gtgaagcact atcaaaaacc gattcttgag caagggttaa tctttttaag tgatgtgatt
    22441 gtggatggaa aaattaattt attgatgcat gatggagaag aatcagacac tacgttgatg
    22501 cctttttatc aatttttaga taataaagat catttatatc agaacaccat ggccttttct
    22561 gctagttctt tcaatccaac ctatagcgag gaaatcaaag gcatcacttg gggattagaa
    22621 tccggcgcca cttttcctgg ctttatcact gttttaatga gcgagctgca caaccctcaa
    22681 gctttcgtaa cccgattaga agaactaatt tgtttggcag atttagacgg ttcgtggtgg
    22741 tggtggccgt atcgcttaga ggctcaacaa ggagaagtcg tgcgagactt tggctgcggc
    22801 aaatgcggct gggcatcggg tctttttgtc agcctaatga tcagtcaata tttcggattg
    22861 aaattcaaaa aaggagtatt gcaaattgat cctgttgata atttaacgtt ttcttggaag
    22921 aatttgaagt tcggtcaagc cttcatttcg attgaatgta cgcaatccga gttgagtatt
    22981 tcaaatttgt cagaaaagcc attgaaaata agtgatacga aaaaaattct tcacgtagaa
    23041 aagggaaaaa cgattcacat tcaaaggaga aaaagatgag tatactaaaa aatattaccc
    23101 aatttaaaaa aatccagagt cgagcaatca ctcccgaaaa tccaacaggc gagaaaggta
    23161 aagctgcgat gttgccaagc gagctaggac caagccgcaa aggtcgtggc gcaattccgc
    23221 tgacttctgg tgaagaaact gtgattgcgg atattactgg aacgggaata attcgtcata
    23281 tgtggatgac gattcgtgat cgaacagaat ttggcagttt tgttttacgt gatttaatct
    23341 taagaattta ttgggatggg tcggatagcc ctgctgttga aagtccttta ggggatttct
    23401 tttgtaatgg gtttggagag cgttgcgagg tgaactcgtt gccgatagtg gttaatccta
    23461 cagggggctt taattcctat ttcgaaatgc ctttccaaac atccgctaaa attacgatcg
    23521 agaatttaca tccgaaggat atgtcctcat ttttttatac gataaattac gctttggtgg
    23581 acgaattgga agaaaatagc ctatattttc acgcaaattg gcatcgggaa cgcattacca
    23641 aattacagga ggattataag ttaattgatc gattaaaggg tcaaggctat tacgtgggta
    23701 cttaccttgc gcttacctgc ctagaacgtt attggtgggg cgaaggcgag tttaaattct
    23761 acattgatgg ggatagtgag tatccgaccg taacctcaac aggttcagaa gattattttg
    23821 gcggggcgtg ggcctttcat gagtacgatg agttcggacg tccacatgcg aaaaactatt
    23881 caacagcctt tttagggtat ccttaccaat caaatagaga tgccacaaga gatcgttttg
    23941 aaacgggaaa attgaatgcc gttcatgcct ttggtgacga cgcaatgcca cgacatggtc
    24001 tttatcgttg gcatcttcaa gaccccatct gttttaccga agatttatcg gtcactttac
    24061 aacaaatcgg caacgatgat gttcgcttat ttgagcggca agacgatgtt gctagtgtcg
    24121 cttactggta ttcaacagaa gcttcaggaa atactaaacg tcatttcact gatcgagaac
    24181 gcttgccgcg ttaatgaagt gtttttcaca tattcgaagg gaggaaccta tgatgatata
    24241 tggggcaatt gaagctggag ggactaaatt tgtctgtgca gtcggtactg aggaattaac
    24301 gattttggaa agagtcagtt ttcccacaac aactccagtt gaaacaatga agcaggtcat
    24361 tgattttttt aaacctttta aggaacaatt ggcaggcatc ggggtcggct gctttggtcc
    24421 aattgatatt caatgcaaat caacgagttt cggtcatatc acatcaacac caaaacttgc
    24481 ttggcaaaat ttcaattttg taggaacgct aaagcaatat ttcaatattc cgattgaatg
    24541 gacaacagat gtcaatgcgg cttgctatgg tgaatatgtt gctggttctg gcaaagggtt
    24601 agcaggtgtt gcatacttca ctgtgggaac aggtatcgga ggtggtgcct tgttcaatgg
    24661 gactttcgtg gaagggttca gtcatccgga aatggggcat atgttgatca agcgacatcc
    24721 aaaggacaat ttttcaggaa actgtccctt ccaccaagat tgtttagagg ggatggcagc
    24781 aggaccagcg attgaaaaac ggttgggtgt aaaaggccag aatttattgg ctgacgattc
    24841 gttttggcag attgaagcct tttaccttgc ccaatgtgca tacaatacaa cactgatgtt
    24901 ttcgccagat agaattattt ttggcggtgg tgtaatgaaa caagaacaca tgaagaaaaa
    24961 agttcaggat aaatttgttg agcttataaa tggatatgtt gaaataccac caattgatag
    25021 ctacattatt acgcccgaat taggtgataa tgccggaata atcggcggat tagcattggc
    25081 cagaaaagcg gtaagaaata agcaaccata attaatgaat gtttcattga tactcatgtc
    25141 aaaagtattt aaccgagtaa tcaaatgatc aaatgaagtt ggataaagcg aaaatgaatt
    25201 caaataaaaa ggctctaagt caaatcgtgt tgttttcatt aaatcactga aaggaaatgc
    25261 ttctgcgttt ccttttttct ttttgttgtg cgctgtatac cttgaggata ttcactggtg
    25321 gcgtacactt aaatagtaat atattgtgtt actttatgtc tgtggtaata gttatagttg
    25381 cttaccaatg tatgttcatt tttcgaaatg ttgacttcat aatccttggc ttaatgtttg
    25441 aatttttttg gtgatagatt ttttcagccg tataaaataa atcgtaattc tattatagag
    25501 agttgtgatt ataaaaagat agcaagaaaa aaaagaaagt ttctatacat tttaaatatt
    25561 ttattcctat ttctattgct aaaaaagtag aaattctata tttaccttta actctcgctt
    25621 ctcttgcaaa tgtagttagc attttaatcg ataata
//
LOCUS       DAEGIQ010000031        24809 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000031 DAEGIQ010000000
VERSION     DAEGIQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.031"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            29..661
                     /locus_tag="KMT45_001166"
     CDS             29..661
                     /locus_tag="KMT45_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004639.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLIFSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(795..920)
                     /locus_tag="KMT45_001167"
     CDS             complement(795..920)
                     /locus_tag="KMT45_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7004640.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1054..1947)
                     /locus_tag="KMT45_001168"
     CDS             complement(1054..1947)
                     /locus_tag="KMT45_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBE7004641.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2129..3055)
                     /locus_tag="KMT45_001169"
     CDS             complement(2129..3055)
                     /locus_tag="KMT45_001169"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBE7004642.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3140..3901)
                     /gene="pflA"
                     /locus_tag="KMT45_001170"
     CDS             complement(3140..3901)
                     /gene="pflA"
                     /locus_tag="KMT45_001170"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBE7004643.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4145..6376)
                     /gene="pflB"
                     /locus_tag="KMT45_001171"
     CDS             complement(4145..6376)
                     /gene="pflB"
                     /locus_tag="KMT45_001171"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBE7004644.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6653..9103)
                     /gene="parC"
                     /locus_tag="KMT45_001172"
     CDS             complement(6653..9103)
                     /gene="parC"
                     /locus_tag="KMT45_001172"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBE7004645.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDSSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9115..11160)
                     /gene="parE"
                     /locus_tag="KMT45_001173"
     CDS             complement(9115..11160)
                     /gene="parE"
                     /locus_tag="KMT45_001173"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBE7004646.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLTVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11326..11760)
                     /locus_tag="KMT45_001174"
     CDS             complement(11326..11760)
                     /locus_tag="KMT45_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBE7004647.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIADDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11887..12525
                     /gene="plsY"
                     /locus_tag="KMT45_001175"
     CDS             11887..12525
                     /gene="plsY"
                     /locus_tag="KMT45_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBE7004648.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12657..13532)
                     /locus_tag="KMT45_001176"
     CDS             complement(12657..13532)
                     /locus_tag="KMT45_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBE7004649.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13614..14405)
                     /gene="codY"
                     /locus_tag="KMT45_001177"
     CDS             complement(13614..14405)
                     /gene="codY"
                     /locus_tag="KMT45_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBE7004650.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14423..15823)
                     /gene="hslU"
                     /locus_tag="KMT45_001178"
     CDS             complement(14423..15823)
                     /gene="hslU"
                     /locus_tag="KMT45_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBE7004651.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNIAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15837..16385)
                     /gene="hslV"
                     /locus_tag="KMT45_001179"
     CDS             complement(15837..16385)
                     /gene="hslV"
                     /locus_tag="KMT45_001179"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBE7004652.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16422..17327)
                     /gene="xerC"
                     /locus_tag="KMT45_001180"
     CDS             complement(16422..17327)
                     /gene="xerC"
                     /locus_tag="KMT45_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBE7004653.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17525..18901)
                     /gene="trmFO"
                     /locus_tag="KMT45_001181"
     CDS             complement(17525..18901)
                     /gene="trmFO"
                     /locus_tag="KMT45_001181"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBE7004654.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18870..20948)
                     /gene="topA"
                     /locus_tag="KMT45_001182"
     CDS             complement(18870..20948)
                     /gene="topA"
                     /locus_tag="KMT45_001182"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBE7004655.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21070..21927)
                     /gene="dprA"
                     /locus_tag="KMT45_001183"
     CDS             complement(21070..21927)
                     /gene="dprA"
                     /locus_tag="KMT45_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBE7004656.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISQ
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKVTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21983..22750)
                     /locus_tag="KMT45_001184"
     CDS             complement(21983..22750)
                     /locus_tag="KMT45_001184"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBE7004657.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22743..23603)
                     /gene="ylqF"
                     /locus_tag="KMT45_001185"
     CDS             complement(22743..23603)
                     /gene="ylqF"
                     /locus_tag="KMT45_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBE7004658.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23863..24261
                     /locus_tag="KMT45_001186"
     CDS             23863..24261
                     /locus_tag="KMT45_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE7004659.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWVMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24498..>24809
                     /locus_tag="KMT45_001187"
     CDS             24498..>24809
                     /locus_tag="KMT45_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBE7004660.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LY"
BASE COUNT         6512 a         5275 c         4276 g         8746 t
ORIGIN      
        1 atacttatca atttaatttg gagatattat ggaaattcaa cgtagaacca ctaatttttt
       61 acatttaggc tctttttttg caactttgac gatcttattt tttctatgtt attcatacct
      121 taagacctca catgcgcctt ctagtgcatt aggctggttg cttttggatc attctgatat
      181 cacagaagcc gaagaaaatc cgtttttttt gattttttct tctctttgcg gcttactgtt
      241 ttctgtttat ttcggtgcct atttttttca tcgacatcct ggtacgttaa acgaagcttc
      301 aagcgtattg attgcattta aaacgttttc ttttttagca attctttttt attcgtttaa
      361 tcacaatacg cttttcttct ttatcatgaa tggtttagtc gtgattttat ccatttttac
      421 ttattttatt ttttcactca tttttagcca attgaaatgt ccgctcttct ttcgattagt
      481 tcctgtcacc tgttttattt acagcttgtt ttcctttttt gtccttgctt taatgccact
      541 gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg gatatgctct tcatcgtcag
      601 tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat aaaaagcatt ttgaagcctg
      661 actattacga cttcataaat gcttttttag ttttaaacac ttcaatctaa aagaataacc
      721 caagattgga taaattatta atgactcgcc attcgtttct tagtgattct ggattatcaa
      781 atggacttcc taatctaatc agctagttct tcccataaaa tgccatactt gactgaatca
      841 taatattgat tatttaaaaa tctaactttt cttatcacta cttcctttgt cattccacat
      901 ttctcaccat ttttttgcat tcctttgttt ccagaccatg tagtgaatct aataggtggt
      961 agataggcaa aatactcaaa taattctttt aaccataaag gaagagaaag gctcctcctg
     1021 caagagtcag tataattttt gcttactgct ctcttaggtt ttactcacta aaactgttcc
     1081 atattctttg atataagaaa atgcatgcgg tataaatttc ttgctcagct cagaaaagat
     1141 ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa ggatccaatg ttggtaattc
     1201 aatctcaaaa ttgttttttt cgacaatagg taattctaat gtgaatgatt cttcttcctt
     1261 tatagtattc gtcacaagta gagactgatg gaaaatacta ggccggcgat ttttatattt
     1321 attcttacgt atcctgtcaa cgataacttc tatttgatca aaaaagtacg attcatttac
     1381 gtctccaata agttcggcta attctttgaa tgccttcgtt acaggaagct gattcttctg
     1441 tacaaaaaag taattagcca aagtaagcgg gaagtgttct atagaggaaa gtgtgataaa
     1501 acggtacttc tcatttcctg cagttgtcct cagcaattct gtctctcggc tgtttcttac
     1561 tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa tacgcccaaa taacttctcc
     1621 catcgctaat ttttgttcat caacagagag tctcttcaat tgttcagcta tggtttcagc
     1681 agccgaagtg gcttgttgca agtaatcttt aggatcaaat attggaaagt tcaaatgata
     1741 tcttcgttct tccctcttga ttaatcctgc atcgatcaat aaatcaagaa aatgggaaaa
     1801 tttattttgc gaaaattctt ttttcaattc tcttagaatc gtttcttgtt ctcgatgttc
     1861 atatagataa tcggtcaatg aagaaaaaag cggatcatcg gtcagttttt gataaaagtt
     1921 ttccttattt tttgaataat agttcatata tgttctcttt tctatccgtt ttattttact
     1981 taaaaggctt ttacgtaaaa aggaggaaga acaagagtca taattgactg ttattctcct
     2041 ccatttcttc ggcctttagt atgttctatg tttcttttta tatcaatcgc ttatccattc
     2101 attttgaatg cttcatacta ccatttgatt agctaaatac agccgtcaat tgaggtacga
     2161 cttgtttttt acgtgaaacg acacctttaa gcaaagcacg gttgttggtc aaagttgttt
     2221 ggaaagcttc ttctactttt tcttttcctt taccagcaac aagtaattct gaatcactgt
     2281 ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc atttgctttc atttcatctt
     2341 ccattgctgc ttctagctct gcttgtcgat ccatcacttc attaaggtcc actgtattga
     2401 cttgagcgat acgaacgttg gaactgccca ttgggaaact ttttgcatcc aaatcaagaa
     2461 gaaccgcagc tgatttatca cttagatttg ttcctgcttt caacatttca agtccataag
     2521 tgtttagatc gacttcagcg attgctgcaa gttcttttgc agctgcttca tcttcaggcg
     2581 tacatgttgg tgatttgaac aacaatgtat ctgaaatgat agctgagagc atgattcctg
     2641 cgatttcttt aggaattgtt acttggtttt ctttatatag ttttaaaata atcgtacttg
     2701 tacatccaac tggttctgca cgatagtaca atggattagc tgtttcaaag tttgcaatac
     2761 gatggtggtc tactacagct aaaatattta cttcgctgat atctgaaata ctttgttgga
     2821 attcattatg atccacaagc atgacatctg cattttctgc tttagctgac tctactactc
     2881 gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt ttcttcatta ggtgtaccta
     2941 gagcaactgc ttctgtttct ttacctaatt cattttgcaa gtgtgaaaac gcaatggctg
     3001 ccccgattgc atctgtatct ggattttggt gaccaaaaac taaaattttt gacatgtcat
     3061 tcgctccttt gtgatttctc ctatttaata atagcaaaaa aacggaaaga agcaatactt
     3121 atcttctttc cgatgcgacc tatcgcgtta aatattttgt atactcttct gtatgaagca
     3181 atcgtttagc attttcgaca cgttcttttg taggtggttc gatcccttct aatggatatt
     3241 tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg ataaggaaga atctctactc
     3301 tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg gatcaaatat tcatcataat
     3361 cacttcgctc aggaactagt acatggcgaa tccaaaccgg tttgttgatt tttgacaaat
     3421 attgagccat ttctaaaata ttttcattgt cccaatgcgt tagtttttta tgctcttcat
     3481 tatcgatgtg tttgatgtca aataacagta aatctgtata cttcatcaat tcttgaaaac
     3541 gtgaaaagaa aggttcttca tacgtaaaag gttttccgca tgtatccaat gtagtatgca
     3601 tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa atcgatctgc aatagaggtt
     3661 ctccaccact aacagtaata ccacctttat ctccccagta aggtctgaag cgttctgctt
     3721 tatctaaaag ttcatctgct gtgtattctt tccctccgcc aatattccaa gtatctggat
     3781 tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa cacgacaaag cgcagtccag
     3841 gtccgtctac tgaaccgaaa gtttcgatag aatgaacata gccgattgtt ttttcttcca
     3901 tttaagttca catcttttct ttgatttcat tttaagtaca agttagctgt cgaagcgaag
     3961 ctttctgaac aaaatttttc gcaattgccg cttcgttatg tgtaaaattt acagggacgg
     4021 aacatacaag tcctgcccct gtaaatcgtt tctttcaaat aagcatactg attattcgat
     4081 tttgatgcga tggctattca acgtcctttc gacgctaagt cgaaaaactt ttttcacgaa
     4141 ctttttacat tctgtcgtgt gaagttctca tcaagacatc taattgttgt tctcttgtca
     4201 agtcacggaa tttaactgca taaccagata cccggatcgt caagttcgga tatttcattg
     4261 gattttccat tgcatcacgc aataaatcat tactgaatac attgacattc aaatgataag
     4321 ctccttttga gaagtatcca tctaagacat tacgcaagtt ttcgattctt gtatcttgtt
     4381 cttttccaag acctgaagga ttgattgttt gggtatttga aattccatcc aatgcacagc
     4441 cgtaattcat tttagctgtt gagttcaggc tagctaataa cccactttgt tccgcattgt
     4501 aacttggatt tgctcctggc gcaagtggtt ttccagcttc acggccatca ggagtatttc
     4561 ctgtattttt accgtaaaca acatttgaag taatcgtcaa taatgaagtt gttggttttg
     4621 agttgcggta agttttatga cgtttgattt gtcccatgaa gtattccaag atccatgaaa
     4681 ctaattcatc tgcacggtca tcatcatttc cgtatttagg gaagtcgcca tccacttcaa
     4741 aatcaactgc tattcctttt tcattacgga ttgcttttac ttttgcatat ttgatcgctg
     4801 acaagctgtc tgccgcatga gagatcccag caatacctgt agcaaatgtg cgttgcaagt
     4861 cagaatccat caaagccagc tgagctgctt cataagaata tttatcatgc atataatgaa
     4921 tgatgttcaa cgtattgaca tatagttctg ctaaccaatc catcatattc atgaagttat
     4981 gtttcacttc ttcaaagtct agtatttctg atgtgattgg acgatattct ggtccaactt
     5041 gggcgccagt cacttcatcg actccgccat tgatggcata taatagtgtt ttagccaagt
     5101 tagcacgagc gccaaagaat tgcatgtctt ttccgacaac tgttcctgat acacagcaag
     5161 caatcgcaca atcatcagaa ccccattgtt cacgaagctg attatcattt tcgaattgaa
     5221 tcgaagaact ttcgatagcg attctgctag catatgttct gaatccttct ggaaggtgtt
     5281 cagaatacaa aacagtcaag tttggttctg gtgaaggtcc catatttgtt aatgtatgaa
     5341 gcagacggaa atcattcttt gtaactaatg aacgaccatc taatcccata cctgcaatgg
     5401 ataaagtagc ccagataggg aatcctgaga ataattggtt atattcaggt gtacgcgcga
     5461 atttgaccat acgtaatttc attactaagt ggtcgatcaa ttcttgtgct tctttttctg
     5521 tgattactcc attatccaaa tcacgttgga tatagatatc aaggaatgct gagatacgtc
     5581 cgattgacat cgcagcacca ttttgtgatt tgattgctgc taaatatccg aagtatagcc
     5641 attggacagc ttcttttgca tttgcagctg gtttagaaat atcatagcca tatttcgcag
     5701 ccatttcttt catttgcgct aatgcacgat attgttcagt gatttcttca cgtaaacgaa
     5761 tcacttcttc actcattgta tgataaccag tcatatcatg atcttttttc ttttgttcca
     5821 tcaaatagtc gattccatac aatgccacac gacgatagtc accgatgata cgtccgcgac
     5881 cgtaagcgtc tggaagccct gtaatgattt tgtttttacg agcagcacgc atttcagcag
     5941 tataagcgtc aaatacacct tgattatgtg ttttgcgcca gtcagtgaaa atatgtgtca
     6001 attcatcgtt tggtgtatag ccattgcttt ttagcgcatt gttagccata ttgattccgc
     6061 cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg tagaccgacg attttttcca
     6121 aatctttatc caaatagcct gcatcgtgtg aagtaatagt tgatacgatt tctgtgtcca
     6181 tatcatagac accgttacgc tcgaattgga tatcatttaa cttttgcaat tcatcccata
     6241 atttttctgt tgcttctgtt gggccttcca aaaagctgtc atcaccgcga tattcagtgt
     6301 agttattttg aataaagtct ctagtgtcta cgccttcttt ccatttggta cctttaaatc
     6361 cattccattg ctccattgta tggacctcct aaatttctgt tttatgtaac agtttttacg
     6421 tgattagtat aacacagcac cctatctttg caagggtttt cttgtttttt atttaacaat
     6481 ttttatgttt tatttacata tatttaaatc aacgttgaca taccaacatt tcagcattgt
     6541 gaacactttc atcttttttt gcaaacgtat aaggatttat taaaaatgaa tgaaaaagag
     6601 acgccacctg tattaataca gatggcgtct cttcattaaa atactacaac agttacgact
     6661 cgatgaaaat ctcaggagca tcgataactt cgatgacttc tccacctttt ttctcatcca
     6721 tcgcaaatga tccattcgat gttcgatcac ctatagggaa atcagttggg tcaatcgtta
     6781 actgctgccc tttttgattc atcaaggtta tttcttttga tcttccttcc gacatataga
     6841 taatacggtg cggatttttt ttcagctcac gcatgaccgt cactccccgc ttcgcacgtg
     6901 aagtttggct gatttcttga gcaagcatgc gtttaacagc tcctcgctga gtaacgatta
     6961 cgatcggcgt atcaccttct gctaacacaa gcagcccgtt gataatccta tctccatctt
     7021 tcaaattcat agacttcact ccagctgcct ttgctccaac aacgggaaca tctgttaaag
     7081 gatagcgcaa acccattcct tgatgcgtta ctaagaaaac atctaagcct ggctgatctt
     7141 ctttcagata cacagcaatc agttcatctt cttccgcctt tagcttcata cagctgagcg
     7201 gacggctctt ataggtgcgc catggttcga actctgtcat tcgtgtttgc ttgatcaatc
     7261 cattcttgct gatgaataca aatgttttct ctgcttgaat tttttgatat gggaaaacag
     7321 ctaaaatcga ttcgtctaca gacagattgg agattgtttg agaaatgtgc tcaccagcat
     7381 ctttccattt caaatcgggt agttcatgga cagggcggta gatcacattt gctttatttg
     7441 tgatcagaag aacatggtcc aatgtactca attcacctga gtatagcaag tagtcgcctt
     7501 ctcgcatccc gatttcttct ggtttggaag cactataaga acgaaggctt gttcgcttca
     7561 catagccttc tcgcgtcaca ctgacaatta cgtcttcttg tgccactaag acttgcgtat
     7621 caattttgat ttcttcgatt tcgtcttcga ttgtcgttag acgagcggaa gaataatttt
     7681 ttttcacttc tcgcaattct tttttcataa cagagaaaag ctctttgtca tttgaaagga
     7741 ttttattcaa ctctgtgatt tgcgcaatca agctttcgga ttccttttga agctctgtga
     7801 tatccgtgtt cgtcaaacgg tataattgca atgtgacaat tgcttctgct tgttcttctg
     7861 taaattgaaa gacgtcgact aaattctttt tggcgtcttt tttgtcttta ctttcacgaa
     7921 tcgttgcgat tacttcatcc agaatcgata aagctttcat caatccttct acgatatgct
     7981 gtcttttttg agctttggct aaatcgaatt gactacgttt tgttatgact tgctttcgat
     8041 gctcgatata acttcttaaa atgtctttca gtccaacttg atgcggagtc atatggtcga
     8101 tagcgaccat attgaaattg taattgatct gtagctcagt gtttttgaac aaataattca
     8161 aaattccttg agcattggcg tcttttttca attcgacgac aatttgcaga cctgttctat
     8221 cactttcatc tcgtacttca gctattccat cgattttctt attcaaacgg atctcatcca
     8281 tttttttcac caaggtcgct ttatttactt catatgggat ttctgtgatc acgatttgct
     8341 gtttattacc cttcattgcc tctatctttg ttttcgaacg aagaataact tttcctcgcc
     8401 ctgtttcata agcttttttg atttcatctt ttccttgcaa gatccctcct gttggaaagt
     8461 caggtccagg aatatattcc atcaattttt caaggctcgc ttgtggatga tcgatcatat
     8521 agattgtgcc atcgatcact tcagctaagt tatgagtcgg gatttctgtt gcatatccag
     8581 cagatatacc tgtcgatcca ttgacgagaa ggtttgggta tcgagctgga aggactgttg
     8641 gttctttttc tgtatcatca aaattccaca cgagatcgac tgtttctttt tcgatatctg
     8701 ctagcatctc tccacttaac ttggataatc tggcttcagt ataacgcatc gcagcaggtg
     8761 gatcaccgtc catactcccg ttattcccgt gcatttcgat cagtacttcc cgcaacttcc
     8821 agtcttggct catgcggacc atcgcttcat aaatactgct gtcaccatga ggatgataat
     8881 tccccatgat atttccgaca gattttgctg atttacggaa tcctttgtca tacgtattgc
     8941 catctttgtt catggcaaat aatatccggc gttgcactgg cttcaatcca tcacggatat
     9001 caggcagtgc acgttcttgg ataatatatt tggaataacg tccgaagcgg tcacccatca
     9061 cttcttctaa agttaattct tgtacttctt gtcgattttc catcaggtga cctcctattc
     9121 tacttcaaat aactgattgt tttcattttt atctgctcgt tcttcatcta ataaatcatt
     9181 agaaactgaa ggagagattt cagtatcctc ttttttatct aagatactgc catcttcttc
     9241 gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga ggttctactt tgtcacccat
     9301 cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg atacggacgc ggatcaacgt
     9361 acgggaagtc ggatccatcg ttgtttccca caattgttcc gcattcatct ctccaagtcc
     9421 tttatatcgc tgcagcatat atcctttgcc gactttttta atcattgctg ccaattcgtc
     9481 atctgtccac gcatattcaa tgacttgctt ctttccttgt cctttggata ctttatataa
     9541 aggcggtaac gcgatatata ctttcccagc ttcaatcagc ggtttcatat aacgataaaa
     9601 gaaagtaagc agtaatactt gaatatgggc gccgtctgta tccgcatcgg tcatgatgat
     9661 tactttgtcg taattacagt cttcgataga aaactcagga ccgactcctg caccaatcgt
     9721 atagatcatc gtattgattt cttcattttt caagatatct tgcattttag ctttttcggt
     9781 attgatgact tttcctcgta aaggtagaat tgcttggaac tttctgtctc ttccttgttt
     9841 agcagatccg ccagcagaat ccccttcgac taaatacaat tcatttttct tagggtttcg
     9901 ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg ccttttttac gcttcttccc
     9961 atttcggctt tcttctctgg ctttacgtgc tgcttctctc gcttctctgg ctttgattgc
    10021 tttacggacc aacatctggc tcatttcgct attttcctgc aaataaaagc ccatttgttc
    10081 actgatgacg ttatctacta cggtacgagc aactggcgta ccaagttttt cttttgtttg
    10141 cccttcaaat tgcagaagat tttcaggtac acggatagaa agtacagcag ctaacccttc
    10201 tctgaaatca ctaccttcca gattcttgtc ccgttctttc agcaaaccaa cttttctcgc
    10261 atattcatta taagatttcg tcatcgccgc tttcatacca gcttcatggg ttcccccatc
    10321 ttttgtccga acgttattga caaaagataa aacattttct gaataaccgt cattgtattg
    10381 ataagcgacc tctacttcga taccttcttt ttctcctgaa aaataaacaa ctggtgtcaa
    10441 tgtatctttt tcttcattca gataatcaac aaattctttg attccttctt cgtaatgaaa
    10501 aatttccttg actggttctt ctccacgaag atcagaaaga gagattttaa cgccttttag
    10561 aagaaaggca gattctctta accgttcggc taaaatttca tatgaaaatt tcgttgtact
    10621 gaatattgta tcatctggta aaaaatggac agtcgtcccg tttgcttttt ttgtttttcc
    10681 tatctttttc aacgtaccgt caggtttacc gccgttttta aatttctgct gatactctac
    10741 gccatctcta acgatcgtta cagttagcca tttcgataag gcatttacca cactggctcc
    10801 tacaccgtgc agtccaccag aagttttgta ccctccttga ccaaactttc cgccagcatg
    10861 cagcacagta aaaatcactt caactgttgg tattccggaa gcatgcattc ctacaggcat
    10921 cccacgtccg ctatctgcaa cagttatact attgtcttca tggatggtca catcaatctc
    10981 actgccataa cctgataatg cttcgtcaac tgcattatcg acgatttcat agactaaatg
    11041 atgcaagcct cggctgtcag tggatccaat gtacatccct ggtctttttc taactgcctc
    11101 taatccttcc aatacttgga tggacgagtc attgtattcg ttgtttactt tttttgccaa
    11161 ggcaaacgct ccttcaataa tactttgcgg caaaaccgca ccacgattat catactagaa
    11221 aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt taattatctc ctaaccgctt
    11281 tttcgacatt cccctctatc gatcatctct tctttgtggt catttttatt tttggttttc
    11341 tttcaactta ccaagttcaa tcttaataca acgatccatt acgatatctg tacgtcctgc
    11401 ttttcttagc atctcagctg cttctttgtt ttcaatccct aattgtgccc agaacacttt
    11461 agcatcagtt ttcaaaaaat catctgctat ttcaggtaaa aattcactcc gtctaaatac
    11521 atctacgata tcgattggat catcgatttc tgtcaaattc gcatagactt tctcgcctag
    11581 aatttcttca ccagccaata atggatttac tggtatgatt ttatatccat tgtcttgcaa
    11641 tacctgtgca accatgtaac ttgttcgttc tggtttattg ctcaagccta cgacggcaat
    11701 attttttgct tcttccaaat agttgaaaat cgtttcttgt gttggatttt ttacagacat
    11761 ttctgccacc ccttgctttt ctttttattc catctattat acttgtatct ttcatttttt
    11821 caattgttcc tgttacctat tcatgctaaa ataaaggata gttttcaact tgaaagaggg
    11881 taaaaaatga aattgatctt attacttatt ttagcatatc ttttaggttc tattccttcc
    11941 ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc gacaacacgg gagcggtaat
    12001 actggaacga ccaatacatt tcgtgtatta ggcaaaacag ccgggattgt tgtcctgctt
    12061 atggatattt taaaaggtac attggctact tgtttgccga tgatcttcca cctttctggg
    12121 attaaccctc tttggtttgg ggtatgcgcc attcttggcc atacatttcc gatttttgct
    12181 gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa tgcttttagg atttaatcct
    12241 gtattttttg tttattcttg tatcatcttt atcgttagtt tattttgtac aagtatggtg
    12301 agtttgagca gtatgatcag tgcagtattg atcactttat ctacgatcgt ccttccttat
    12361 gtagctcctg tgattttggc taagcccaat tggttactga cgatcattgc ctttttagtt
    12421 tcttccttta tcttttatcg gcataaagac aatatcaagc gtatcagaaa ccatactgaa
    12481 agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat cctgatactt tttcattcca
    12541 aagagataaa acgggagtgt gacaaatcga tgtcacactc ccttattact tacaggatag
    12601 cttttttaca caccttggta aacggtgttc cctgctaact tctcggtcac aacctcttac
    12661 ttcactgtga tggaatattt tgtcttgaag atttcatggg cacctagttg attgatccct
    12721 actttttcca tcaagtttcc actactgttc accgtgtcag cgattccgca ccacggttcg
    12781 atacaaacga aaggcgcttc ttttggataa ggagaccaga tccctacata aggcatatct
    12841 ttatagctta aagttacgct atgggaagat ttttcacttc gaatggcgta cgcatttaga
    12901 ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt cacgattcaa agccagattc
    12961 gtatttgtct gacctaacgt cttttgatcc atatcgatga atgggccttt caatggtagg
    13021 cgtaatcttg atttcaatgg agaaaatgcc agatagaaat cttcaaatga caatccttct
    13081 tcaagaggta cattgaaagc aggatgtcca ccgatagaaa agaacatttc tttttcaccg
    13141 gtatttgcca cttgataatg aaccgtgatg ccgtctcctc ctaattcata gctgatcact
    13201 aattcaaaat caaatggata cttttctaat gtctccggcg tactccttaa gatgaaacta
    13261 gccatttctt gtccatgttc aatgacttca aaatccatat cccgagcaaa tccgtgttgc
    13321 cccatttgat atgttttccc ttgataaaca tactgatcat cttttaaacg tccaacaaaa
    13381 gggaataaaa ccggtgcatg tctgccccaa tacgctggat caccttgcca gatatactct
    13441 aaaccatgtt cttttgattt cagactgatc aattccgcac cgtgttcagc gatttcggcg
    13501 atcagctggt cattttgaat aataacagtc attgccattc ctccaacttc gacttacttt
    13561 ttaaaatggg aaggctggaa caaacatttc tatctgcccc agtcccctta cttctaataa
    13621 ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata cttttaaata agttcccttc
    13681 atgcctaacg aacgagattc aatgattcca gctgattcca attttcgtaa agcattgacg
    13741 atcacagaac gagtgatgcc gatactatcg gcaatattag aagcagtcag acgaccttca
    13801 tctccatcca atgcttcaaa gatagcttgt acagccttca attcgctata agataatgtg
    13861 ttgattgcca tttggacagc cgttgcactc cgtacatctg cttcgatgct tcgagacttc
    13921 tgataaagga tctgcatacc gacaaccgta gctccatatt ctgctaagac tagatcgtca
    13981 tctgcaaacg attgttccat acgagataag ataatcgtac ccaaacgttc gccagctcca
    14041 aagatcggaa cgacagtcgt cagaccaaat ggatatttct ctcttgactc taccggaaat
    14101 gcagtaatat cactagtaat tgggatatta gcttcagttt tcatcaactg tgccactgta
    14161 tcagtataag attcaggaaa ttgtttctca acaaacattg ttttgattcg ttcattgttc
    14221 acatcgtgac gttcattata acctagtaat actccgtcac ttcctatgat ataggtgttg
    14281 ctgtcaagaa taccacctag tgtgacagcc attttatcgt aaggcaaatc tgcttgcatg
    14341 tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc gggttttcgt tagcaagtct
    14401 gtcattgtcg tcctccgatt ttttataaaa tatagcgact taaatcttca tttgccgcaa
    14461 ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt tatttctccc atttgcatat
    14521 ctggtgcttc aaataataga tcttctagta gtttttccaa aatggtatgc agtctgcgtg
    14581 cgccgatatt atctgtgtca cggttgacat cgaaagcgat ttgagcgatc cgttcaattg
    14641 cttcttttgt aaacgtaact gacacatttt ctgtaccgat caacgcgacg tattgtttta
    14701 ctaaagcatt atttggttcg gtcagaatct tcacaaaatc ttcagctgtc aaatcatcta
    14761 attctacccg tatcgggaag cgtccttgaa gttctggaat tagatcactt ggtttagaca
    14821 aatggaaagc tcctgaagca atgaatagga cgtgatctgt ttggatgatc ccgtattttg
    14881 tgtttacttg tgaaccttcg acaattggca atatgtctct ttggacgcct tctctggata
    14941 cttctccaga ttgctgtgat ttagaagtga tcttatcgat ttcatcgata aaaataattc
    15001 cactttcttc cgctaatttg attgcctcac tatggatatc agcatccttg actaattttg
    15061 ctgattcttc cttgatcaat aactctaaag cttctttgac tgttaccgtc cgttccactt
    15121 ttttcttcgg tgtcaaagca cctagagttt catttaggtc gatccccatt tgctccaatc
    15181 cattgttcat cattggtgca ggtttctttt ggtcttcaac ttcaatggtt acttcacgac
    15241 tatccagtaa acccttttgt agttgttcca agactgtacg gcgattgatt cgaatatctt
    15301 ctgtcacttc ttcttgcggt tcagcgatat tttgctggct agcattaaaa agattcatca
    15361 tttgttcaaa ttgattagtg gattgtttct gttctttttt gataccaggt accaagattt
    15421 ttaccaaacg tttttctgca ttttttttag cacttgtgta tacacggctg tactgctctt
    15481 tttctacgat ttggatcgca ttttctacta aatcccgaac cattgattct acatctcttc
    15541 cgacataacc gacttcagta aattttgttg cttcgacttt gacaaatggt gcattgacga
    15601 tttttgccaa acgtcgtgcg atttctgttt taccgacccc tgtaggtccg atcatcagca
    15661 tattttttgg tgtaatatct tgttgcattt tttcatctaa ctgcaaacgg cgatagcggt
    15721 tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg tcctacgata tattcatcta
    15781 attcacttac gatttgtctt ggtgtttgtt ttaattcata catatctact cacctcttac
    15841 agttcttcta cgataatatt atgattagta aagatacaga tatctgcggc aatatttaac
    15901 gcattttctg caatttcttt cgcactcatg tcgttttgag catagttttt cattgctcgg
    15961 gctgcagcca acgcaaaatt ccctcctgat ccgatagcta agataccatc atctggtgtg
    16021 ataacttctc ctgtacctga aacaagaagc atttctttat cattcattac gatcagcatc
    16081 gcttcaagtt tttgcataga ttgctgtgtc cgccattctt gagctaattc gacagctgcg
    16141 cgttgtagat tcccgttata ttcgtttagt ttgccttcaa atttttcttc taaagtaaac
    16201 gcatctgcaa cacttccggc gaaacctaca acaacttctc cattgtagat acgtcgtact
    16261 ttctttgcag tgcctttcat gacaacagat tcacccattg ttacttggcc atctccagcc
    16321 atagcaaatt tcccgtcttt ttcgacggca cagatagtgg ttgaatgaaa tggtgattcg
    16381 accatgtttg tttcctccta aatttgttct tttccttatt tttacgctct tggatggaaa
    16441 gaacgatagt ttttttgtaa actttctttt gttacatggg catagatctg ggtagtcgat
    16501 aaattggcat gtcctagcaa ttcttggact gtccgcaaat ctgccccatt atttaataaa
    16561 tgagtagcaa atgtatggcg cagcatgtga ggatggatat cactatccaa actgcttttt
    16621 ttgatcattt gattcaagac atattcaatt ccagtaggcg tgattggttt tccatgatga
    16681 ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca tcaatacctg gcggccttcc
    16741 ctgaaatatt cttgcagagc atcttgagca aaagaaccta gaggagcata tctctctttg
    16801 ttccctttac catggatcaa catgacctct gaatcaaaat caatgtctga taccgacaaa
    16861 ctgctacact cacttaaacg gatgcctgac ccgtaaagga cttccagtaa agcgcggttc
    16921 ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa gtgcttgcat ttcattttca
    16981 taaaaaaaac gtggcagtct caaatttttc tttttgagat gaacgtaaga aaatggattt
    17041 tcttttatca cttcttcttt gagaagatac tgataaaacg aacgtaggct ggctatcttg
    17101 cgactgatag aatttctgct atagctagga cgatgatcag ctaatgcact caaatagaca
    17161 cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc cagattcctc caaaaaatgt
    17221 ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt tttctgaata tcctctttcg
    17281 acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt ctgtcatttt ctcaccttcc
    17341 ttttgtccca gagaataatt tagcacagcg tttataaaaa gacaaataaa ttgtcagaat
    17401 ttaaccaata tttctaagtt tgttcacaat tggcgcaatt cctcacattt tgtttgagaa
    17461 attcttaaga aaaacgcaag aaaaagagcg agactatccg tctcaccctc ttcatggagt
    17521 ttccttactc atttgggatt gcagtttttt ctaattctgt ttttattgcc tcattagctt
    17581 ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt gtctcgaatg cgctctggca
    17641 gttcaggaaa cagaccatag ttggcattca tcggttggaa atgttttcct tccgcatgag
    17701 taatgtagta agccatactt ccaatagctg tttcttgtgg aaactcgact ggttccattt
    17761 cttttgctaa gcgtgcggcg ttgattccag caagtagtcc gctcgctgca ctttcaacat
    17821 agccttctac accagtcatc tgacctgcaa agaacagatc ttcgcgtttc gttgattggt
    17881 aagtcggttt cagcaattct ggcgaattca taaagctgtt tcggtgcatc acaccatagc
    17941 gaacgaattc agcattttct aacccaggaa tcatccggaa aacacgtttt tgctcgcccc
    18001 atttcaagtg agtctgaaag cccacgatgt tgtataaaga agctgcagca ttgtcttgac
    18061 gcagctggat aacagcataa ggacgtttgc cagttttagg atcttccaat ccaactggtt
    18121 tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc cattacttca attggcatac
    18181 atccttcaaa atatttttct ttctcaaaag atttaagttc tgccacttcg gcattgatta
    18241 gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg acagttcaag taagctgctt
    18301 ctcctttgtc atagcgggac ttcaaatata ctttatccat atcgatcgta gctttgtcta
    18361 cgattggtgc agctgcatca tagaaataaa aaccatctga accattgaat gctttgatgc
    18421 tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac tacggtgatt ccttccggta
    18481 tttcagtaat ttcttcgttt atcactgtta ctaatggatg ctctttgact tttcgggtaa
    18541 ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc accagctggc actgctgttt
    18601 catctgccga agtgattact accgaattca atcggcgcat ttcttctttc aataacccta
    18661 ctgcattagc caaactgttt cctcttaatg aattggaaca tactagttca gcaaaattac
    18721 ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc atataaacgg acagggactc
    18781 ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag ccctgctcca acaacattta
    18841 ccattttcaa tgaaagaacc tcttttctat tatttttgga tgtcttcttc gtaatctcca
    18901 tttacacaga ctacttgttt accgcctttg actttcttct cgaccagata tccgtcacat
    18961 tttggacaat ttctgcccac tggtttatcc caagaagtga attcacattc tgggaaacgg
    19021 gaacaaccat agaatagtct gtttttctta gattttcgtt caatgacttg tccttcatga
    19081 catactgggc aagtcacacc gatttctttt acgattggtt tcgtattccg acaatcagga
    19141 aaattactgc aagcataaaa cttcccatat ttccctaatt ttatcaccat cggatgtcca
    19201 cagacatcac aatcgaatcc tgctggttca tctttgattt gaattttttc gattttttct
    19261 tccgcattcg tcagttcttt ttcaaacggt ttataaaaac gatcaaccac tttgacccac
    19321 gcttccgtgc cttcttcaat tttatccaaa tctccttcca tttctgctgt gaaatggata
    19381 tcgacgattt gagggaagaa ttcacagatt aatgaattga caatttctcc tagttcagtc
    19441 ggctcaaatc ttttttgtgt caattttaca tagtagcgtc tttggatcgt atcaagtgtt
    19501 ggtgcataag tggaaggtcg ccctaccccg ttttcttcta gtgcacgaat caacgtagcc
    19561 tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg gttcaatatc aacagattgg
    19621 actacatcgc cttcttcaag ttctggaaga atattttctt tctcttcttt gccgtcatct
    19681 ctaccttcca catacacttg catgaatcct ttgaatttga tttttgaacc gttcgcaatg
    19741 aagatgacac cattttgttc taatgtcact ttcattgtat ctaaaatagc tggtgtcatc
    19801 tgactagcca caaaacgtga ccagatcaat gtataaagtt tcaattgatc tttatctaaa
    19861 tattgtttga gttcatctgg tgtacgaagc acactcgttg gacgcacagc ttcatgggca
    19921 tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat gtctagaaaa ttcttttcca
    19981 tacgtatctt caataaatgc tgcggcctct gcttttgctg tatcggcaat ccgggtagaa
    20041 tcagtacgca tgtatgtaat caagccgact gttccttgtt tacccaaagt gattccttca
    20101 tataattgct gtgctaccat catggttttt cttgttcgaa agtttaactt acgggcagct
    20161 tcttgttgca ggctacttgt agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt
    20221 tctacttttt taacttcgaa gtctgttcca tcaattctag cagtgacttc ttttacactt
    20281 tgtgcatctg gtaatttctt tttcttaccg tcgattcccc agaaatttgc tttgaatttc
    20341 ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt cttctggaac aaattctcgg
    20401 atctcttttt cacggtcaat aataattttt aaagcgacag attggacacg tcctgcactt
    20461 aaccctttct tgaccttacg ccagagaata ggactgatcg aataaccaac gattctgtct
    20521 aaggtacgac gagcttgctg tgcatctact aaatctacat cgatcgttct tggttcctta
    20581 aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga ccacccgatt tttttctttc
    20641 agatccaacc caagtaggta cgctaaatgc caagcaatcg cttctccttc tctatccggg
    20701 tcacttgcca agtaaacttt ttgagctttt tttgctgcgg ctttcaggct tttgatgaca
    20761 tcgccttttc cgcgtataga aatataatgg ggttcataat tgttttcaaa atcgataccc
    20821 attttacttt tcggtaaatc tcttatatgg ccaacactgg ccattacttt atagtttcgt
    20881 ccaagatatt tctcaattgt cttggctttt gcaggtgatt ctacaatcac taaatattta
    20941 tacgccatta ctgaatggac tcctttccat tgtcgtgttc ctactattaa aagaggattc
    21001 atacaaatcg tagctaatca tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa
    21061 agtatggtgt tattctttaa attctgccaa gatatcttca cccgaaaccg tgcaaactgc
    21121 accgtcttgg atcaaatgat gacatccggt agaatgttct gttaaaatat ttcctggaac
    21181 agaaaaaact gttcttccaa gatccaatgc ttgctgtgct gttatcagag agccgctttt
    21241 tttcttggct tcaaagacac atgttacttt agacaaagct gcgataatcc gatttctcat
    21301 tggaaaacaa tgctttttcg ggccggattg aggaggatat tcactcaaaa gaagatgatc
    21361 atttgcaatc acctcttgca agtcacgatt ttcagcagga tagacttgct ctatccctgt
    21421 tccaatgact gcgattgtcc gtccaccctg agagattgct gcttggtgac cgtatgtatc
    21481 tatccctttc gcgcagccac tgacgatcac atatccagct tctatcactg gttttagtaa
    21541 ataataaact gtttttttgg ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat
    21601 gttacaagaa agtaaagata gatcaccatt ataaaacagt acaattggcg gataggtcaa
    21661 gtgaagcagc tgagggggat atttgggatc aaagcaagtg ataaacgact gtttcgattg
    21721 gattttgccc cagttagcgt gaatttcctg ccaacttttg cgaaacagct cccgatgttt
    21781 ggttatttta cctatcttaa tgatgtcttc aaatccgatt tctttataag gatactgcat
    21841 catccagtta gcaagtgccc attttccttg actactgatt ccttttgtat aagcaagttt
    21901 caataaattt tcttctattt gatacatcat aaaaccgcct ttcgtctctc tttctaataa
    21961 gatacgtaaa agcggctgtt ttttattgta aatatttttt tacaggtgca aaacttttac
    22021 gatggattgg cgtaattcct tgtcgttcaa gtccttctaa gtgttccttt gttccataac
    22081 ctgcattatg cgagaaacca tatccaggat aaagcacgtc atattctttc atcaattggt
    22141 ctcgatatac tttagcaatg atacttgcag cagcaatcga cacagatcgt gcatcccctt
    22201 tgattatttt ttcctgctga ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc
    22261 ttggtacaat ggacaatttt tctactgctt ggatcatcgc taatttactt gcttcataaa
    22321 tattcacatc atcgattgtc tgttgatcaa cgatcccgat cccgattgcc agggcttttt
    22381 cttgaacttg ttgataaaga agttctcgct ttttctctga taattgtttg gagtcattca
    22441 gccctaatat gatctcattt ttcggcaaga taactgcagc tgccacgaca ggtccagcta
    22501 atggtcctct tcctacttca tcaatgccgg caatccattg gtgaccttgt gcgtaccctt
    22561 gcttttcaaa gcaaaacatt tcttcatatt tttccttaat taatgcttcc tttttcaatc
    22621 gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg tggatcattt cttaatgatt
    22681 ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg ttctttgata ctttggatgg
    22741 attcagtcat cacttttttg ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt
    22801 tttccttgac gaatatcatg cacgactaat tcgctgccac gatcatagtc ttctttatac
    22861 ccacgttttg ctgtaatatc catcagtaat tccggtgcca atttttcttc ttgctcgatt
    22921 gataaaccat aacgatcgct gattcttcct gaataatatc gggcaaaaaa gtctaatccg
    22981 taaatcgcta aatcatctaa atggacaagc tggtctttga tcgcgcctgt cagcgctagt
    23041 tttttgccga tttcttcatc ttcaaatttg ggccataaaa tccctggtgt gtctaacaat
    23101 tccaactcat tacctaaacg aagccactgc tgaccttttg ttacgccagg cttatttcct
    23161 gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg tagatttccc aacatttgga
    23221 atacctaaga ccattgcccg aatagcgcga ggcttgatcc cccgagcagc atctcttgcc
    23281 cgtttttctt tcaatgcttc tttagccgca ggtactagac ctttaattcc tttactttct
    23341 tgtgccgtga tagctaatgc aagatggcct tgtttacgaa aatattcttg ccacagcttt
    23401 gtctgttgtg gatctgccag atccgcttta ttcaataaaa caaggcgagg tttttgctga
    23461 atgatctgat ctagcattgg attacgagaa gataaaggta atcgtgcgtc taccaattca
    23521 aatacgatgt ccacatattt cattttttca gaaacttctc gtcgggcttt tgccatatgt
    23581 cccggaaacc attggatggt cattgttctc tccattcctt tcataccgga atattccccg
    23641 gctttacttg tttttttctt tttcaatata aattcacttt tttattataa agaaattcca
    23701 tctattagtt aagtgaaagt gaaccagtcc tcattctatc acatcttcac ttttttgatg
    23761 atgcaaattt ttattttggt ttctatatat gaatctaaat ttttgacagc gtttcctaaa
    23821 taagagatga tagacgaaca aaacagaaag gagacgaaac aaatgaatag tatggttttt
    23881 gttaattttc ctgttgctga tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt
    23941 aaaaaaaatg aagaattttc tactgaagaa gctggttcaa tggtatggga tgacactttt
    24001 tgggtaatgc ttctctctca tgatttttat aaaaaattta taaaagataa agaaatagct
    24061 gataataaaa aaacaagtgg tgctttgatt tcgtttagtg ttgaaaacgc ggatgaagta
    24121 aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt tccacgaagt agatatggga
    24181 atgcctgaag aagaaatgtt ttcattagaa gtccaagatc tagacggaaa tacattagaa
    24241 cccgtatgga tgaagatgta agcagataac taatctgacg ttttttataa caaaaaaaca
    24301 aaggcggatg atacgataag tattctccgc ctctattttt tttattcgta caatctcttt
    24361 tttacagttt ataaaagcag tcttctcacc ctttttttaa ttgttagatg ccagtgtatg
    24421 taaatcacca atgtgtcatg acacattggt gattttatgg tatcctttta ttattaactg
    24481 acaaaggaga gaatattttg aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa
    24541 tgaatgcaag attgttgtca acctctgctc ttttattagc ccttgatgtc atcttataca
    24601 agatagcgat tggtcctgct tattttcaaa tcacattcgg atttttaagt atggcgctta
    24661 tgggttatct atatggaccg atctgggctg gtttgcttga aatgttgtcg aacactttaa
    24721 atttcacgat atttggttca ggaacgtttc aagctgcttt tcttatcacg gctttccttg
    24781 gtggatttat taatggctta tttttatat
//
LOCUS       DAEGIQ010000032        24350 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000032 DAEGIQ010000000
VERSION     DAEGIQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.032"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            184..501
                     /locus_tag="KMT45_001188"
     CDS             184..501
                     /locus_tag="KMT45_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004661.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            504..736
                     /locus_tag="KMT45_001189"
                     /pseudo
     CDS             504..736
                     /locus_tag="KMT45_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(851..1330)
                     /locus_tag="KMT45_001190"
     CDS             complement(851..1330)
                     /locus_tag="KMT45_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004662.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            1450..2787
                     /locus_tag="KMT45_001191"
     CDS             1450..2787
                     /locus_tag="KMT45_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBE7004663.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            2810..3337
                     /locus_tag="KMT45_001192"
     CDS             2810..3337
                     /locus_tag="KMT45_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004664.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            3324..3887
                     /locus_tag="KMT45_001193"
     CDS             3324..3887
                     /locus_tag="KMT45_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004665.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            4309..5046
                     /locus_tag="KMT45_001194"
     CDS             4309..5046
                     /locus_tag="KMT45_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004666.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(5063..5479)
                     /locus_tag="KMT45_001195"
     CDS             complement(5063..5479)
                     /locus_tag="KMT45_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004667.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            5872..7119
                     /locus_tag="KMT45_001196"
     CDS             5872..7119
                     /locus_tag="KMT45_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBE7004668.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            7387..9777
                     /locus_tag="KMT45_001197"
     CDS             7387..9777
                     /locus_tag="KMT45_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBE7004669.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            9826..11661
                     /locus_tag="KMT45_001198"
     CDS             9826..11661
                     /locus_tag="KMT45_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBE7004670.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            11730..12593
                     /locus_tag="KMT45_001199"
     CDS             11730..12593
                     /locus_tag="KMT45_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBE7004671.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            12583..13692
                     /locus_tag="KMT45_001200"
     CDS             12583..13692
                     /locus_tag="KMT45_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBE7004672.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            14031..14831
                     /locus_tag="KMT45_001201"
     CDS             14031..14831
                     /locus_tag="KMT45_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004673.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            14919..15725
                     /locus_tag="KMT45_001202"
     CDS             14919..15725
                     /locus_tag="KMT45_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004674.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            15957..16826
                     /locus_tag="KMT45_001203"
     CDS             15957..16826
                     /locus_tag="KMT45_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBE7004675.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            16846..17193
                     /locus_tag="KMT45_001204"
     CDS             16846..17193
                     /locus_tag="KMT45_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004676.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            17206..18468
                     /locus_tag="KMT45_001205"
     CDS             17206..18468
                     /locus_tag="KMT45_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004677.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            18548..19924
                     /locus_tag="KMT45_001206"
     CDS             18548..19924
                     /locus_tag="KMT45_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBE7004678.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            19921..20550
                     /locus_tag="KMT45_001207"
     CDS             19921..20550
                     /locus_tag="KMT45_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004679.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            20569..21048
                     /locus_tag="KMT45_001208"
     CDS             20569..21048
                     /locus_tag="KMT45_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004680.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            21550..21603
                     /locus_tag="KMT45_001209"
                     /pseudo
     CDS             21550..21603
                     /locus_tag="KMT45_001209"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21607..22206
                     /locus_tag="KMT45_001210"
     CDS             21607..22206
                     /locus_tag="KMT45_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBE7004681.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            22222..22698
                     /locus_tag="KMT45_001211"
     CDS             22222..22698
                     /locus_tag="KMT45_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004682.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            22715..23617
                     /locus_tag="KMT45_001212"
     CDS             22715..23617
                     /locus_tag="KMT45_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004683.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            23690..23884
                     /locus_tag="KMT45_001213"
     CDS             23690..23884
                     /locus_tag="KMT45_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004684.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            23989..>24350
                     /locus_tag="KMT45_001214"
     CDS             23989..>24350
                     /locus_tag="KMT45_001214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004685.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVA"
BASE COUNT         9115 a         3433 c         4890 g         6912 t
ORIGIN      
        1 tatttacggc gaagtatatt catattcgct tgaaggtgag aatattgagg atttacggtt
       61 gattggtgag tagaaaaaaa cagaaatatc aaaatggaga taaaaattaa tagcatgtaa
      121 agatagtcat gcttgggtta aaaatgttaa aattcaaaaa aatgagtcag aagggataaa
      181 agtatgaaaa gagaggaata caaacaaaga ttaaacgaac tgcttgaaga ggacgaaaca
      241 ttaactcatg gttcgcctga tgaaattcta tacatgattg ataatatggt gatctttggt
      301 ggatatgaac ttggtaatag gtcagttgat cataatattt tagaatttga tgatgtatca
      361 tgggaagaaa ttcttgattg gggaattttg gcggtacctg aaactaaaac ctatattagt
      421 gacactatgg ttcctttttt tgaggaattg gattataaaa gacttcccaa aaatgaaaat
      481 catatactgg gaggaaacta acaatgttaa gtgacttatt tattttactg atctatctag
      541 gtgtaagtga aattattgcg tattttttgt ttgtcattgc taattatggt atttccagtg
      601 caatcgttca aatgtttaga ttaccagtaa gtgaactaaa aaaatgatga gattatcagc
      661 atatctttta atgtttttga atgtggtcac gttaatcttt gtagtaatcg gtgtgacttt
      721 cgatttaatg gaatagtggg aaatctataa tggaaaaaag agacacgaat gaacatattt
      781 tttgaatcaa tattgttgat ggtttttgcg atcagcgctt accagaaatc taaaaaagag
      841 taatgattgt ttaatcatca ctcttttttt gtagggaaat tgaaatatgt ggagcttgta
      901 ttggtttagt taggattgct tggctataag gagctagttc tttatcaaaa ccatcaaagt
      961 aggttttcag tagcgattta actgatcgct catcttgttt gatttggata atttcagcat
     1021 ccaaatcggt tgtgagatca ggagcttttg atgcgattac ctgatttttt ttatcttcca
     1081 actctcttag gctgtttaac aagttgtaaa aagaatcatt ttgattgttc attccaacgt
     1141 gagttgcaat tttaacagaa gtttcgtccg tattgtcatc aaaagaactc attggaatat
     1201 caagtagtaa cgaggatgtt tcttttactg actttttctt tacagcattt tttgtttttc
     1261 catctttttt gctctttact cgagatgttt ttggagctag tttaggtaaa tcacttgttt
     1321 tatcagtcat ttattagctt ttcctttcta ttaatttatg ttattatatt atcataataa
     1381 cataaaaaag tacacttttt gggggcgtag ttattgaaaa aatctaagag aaagattgcc
     1441 acattgctta tgttaatggt gcaatttaca gggattgcaa tgagtgtaat cacaataaat
     1501 ccgcaagtga tacatgctga tgggatagct acttatgatg cacaatttaa ctgtactact
     1561 gaaggtagtg ggtcaggaac agatagcgat ggaaacgata ctggaagtgg cgctgtaaca
     1621 ggtgatgtag ccaaaaatat tgaagatatt tacaaataca tgaatggaga atatggctta
     1681 tcaggtgcta gtattgcagg tattttaggt aactggaaac aagaatcagg tattgatcct
     1741 cgagcagtag aagggtatta cggagatgga cagagagaag ttgacttcat caaacaattt
     1801 actgatggtc tgatgggtat tggctatggt caatggacag ctgggcgaca tacagcatta
     1861 gttgattggt ctaagaaaaa taatggcggt tcttggtgga ctacagaagc ccaattggac
     1921 tttatgttca aaggtgatgg gggattcgta ggaatattaa aagattatgc tttaaatgct
     1981 tctgatgatg tcgtagaaaa tgcagtaact tttcataaaa actgggaaat ttcagctgat
     2041 tccgaatcag ctgtaatagc tgtacgtggt gaaaatgcaa aagaaatctg gaagtacatg
     2101 aaaagcaagg gtatggacgg tgcaaaagat gaaagtaaga tcaataaaat atccggaggt
     2161 tcaagtaccg gagatggatc gaataattca tcaagttcag catcaggaac agtagaagat
     2221 gcctgtgcaa ctacggatga cgctaattct ggttctggat caattgggaa agaaggaaaa
     2281 attaacggta aaaaaggtca agtaatagcg aatatgactt atgaagaagc tttgaaaaaa
     2341 tatgggaaag cgatcacgct accaaaattc aaagaaccag attggtcaac ttcacctttt
     2401 aacggaatat atcaaggtca gtgtacagag ttgacgtggg cttatatgag ccaattatac
     2461 gatgggaatc aaccgactac tggaaatggt ggttatgtat gggaaagcta caaatcggca
     2521 ggagcaaaaa taacgaacaa accaatcgta ggttatgggt ttagtgcttc ggatggatgg
     2581 ttatgggctg ttggagatgc agggcatact ggtgtagtta tagcggtgtt tgaagatgga
     2641 tcatttctat gtgctaactt taatgttcca ccaacccaag ctccaacaag gggagtttcg
     2701 gtaacgttga ttgatggagt ggacggatca gataatgtgc atttctttag cggtgtagga
     2761 aaagctaaat ttgaaatcga aaaataaatt ggaatgaagg acgatataga tgaatagtaa
     2821 agtgaaagat acaattatca tttctttgct ttctggtatg ttattagcac tgcttgttgg
     2881 aatcctattc ttcaataagc agaatcagga catagaagct gaaaaacaca gtgtgcatat
     2941 tgaagataag ctgacattct ttaaagaggt taaaccaaat gaggtggatg gattacggaa
     3001 agatttagaa aatgatattc aagaaatgtt gaatggatca gttcaaaata agtttgcaga
     3061 tcaatcaggt gaagaatttt tgagaaaact atttattgta acaggtaaac caccaattac
     3121 tgataagagt agtgagaaag aaaaaatcga gtactataag ccttttactt ttaagctaac
     3181 aaactttgga attaagacat ctagtagtgg gttgtatgaa gcattgaccg attttgaagt
     3241 tcaatacaat ggaaaaaaag tgtatgatac cacattaacc atcacactaa ataatgataa
     3301 gaaagtgact gggggtgctt tttatgcaaa atcatgaaat cactactgct aaaaaggcga
     3361 tcggaatact ttccataatt gtgatccttt gttgtgtggt actatttcaa aagaaaaatg
     3421 cttatgataa taatgtcaaa gaattggaag accttaaaag taaacaaatc gaagtagtca
     3481 agaataacca gaaagtgaaa gctatgcagg aagctcaata caaagaattg ggaaatgctg
     3541 aaattaagga acaaagtagg aaattcttta atttatttta caactggaat tcatgggaaa
     3601 aatatacgaa gaatatgaag gaaatacaaa aagaatttcc tcagatagta aagtctagtc
     3661 ctgttgatat ttctggcacg atggtaggca ctggtaatag tccaatatct agctatactg
     3721 ctgattacta tacaacaaat caaaaaggta aaatggttga attgattaca cagacaagaa
     3781 atactccaac aacagcaaca acaacaattt ggcgtggagt aacaagcata ggagaaaatc
     3841 aattgtttta tttggaggat ttagatagct atgtaagagc agagtaaaat aaaaaaagag
     3901 ctaacggtac caacgttagc tctttgtgct gacgatgata acaaaaccta ccgaaaaagg
     3961 attgtttcat cacaaacaca aacagaaaag aattgataaa aaacccgaac ctcagaattt
     4021 ttattaatca attcatttag attataataa attgagctga acattacaat acgtatatgt
     4081 tgtaaaacgt aaaaaaagtc aaaagtaaat actttcttaa aaagtcaaat aaaactatta
     4141 aagacaatat atatggttta tttgacataa gaaaatggtc tagatgataa ttgtaatata
     4201 gaattgtaga cttaaataaa gcataaaata ccttttatga taaggtatag ctcttaattt
     4261 taagcagttt tagatataag attgaaagta aagtggggga gaataataat ggcagatcat
     4321 aaattagaaa agttattaga agaaacgtgg aatccaaaag aatttagtga attttttatg
     4381 gagaattttg aaactgactt agcagtgatt gttaaagatg ctcttagaga gcaagggtat
     4441 cctgaaactg caaactatat aaacattaat tttactttgt atactgaaaa taaaggaact
     4501 tgggattttt gggctacttt agcaaataag gagttaagtg ataaatcaga cacaggaatt
     4561 cgtaactttt ttgaatcaaa cagagatgat tatatgtatg ctaacaatca aaataaattg
     4621 aactttagag tagagtttga tgaaacacct gaagaattta tagaaagaca accgcctaaa
     4681 gaaaatgtag caaaggtatt ggaagatcgt tggaattcag atgaaatagt ttcaacaatt
     4741 agcgaactag gcggacaata tgaaccgcta gtagaagcgg ttagagaaga gctaagactg
     4801 aataagtttc ctgatgttca aaatattgat gtttctcaaa ttgaaataaa cgtcaagatc
     4861 acgaataaac ttgattatgg tagttgggca gatatagcat tagaaaaata catctactca
     4921 acgttaaaag agtttatcga aaatagaatg gatatcatgt atctacagca tccacagtat
     4981 ttaaactttg gtgttgaaat agctacacct cttgaagaat ggaaaatgga gcaagggctt
     5041 gactagcctt tgctccattt ttttatttta caaatgaata cagttcgtcg tttttaccaa
     5101 gaatgattgt ttgcccaact ttgagattgt caggtgatcc ttcagcggtt tgtaaggaga
     5161 acgttccatt gaacttatcg gataccgtta taggtagctt taattctgat ccttcctcat
     5221 ctttgagaat tgctgttttt cgttcatcaa aataaacagt ctttattaaa taatttttcg
     5281 tagtgatctc agcagatttg tctttttttg tagtagtacc agtttcttta gaattagagg
     5341 tattttctac gactattcca tctttggcat ttgagcttgt gtttatttga ttgaatattt
     5401 tcagcgacaa gataacgcta agaatagtca atatagaaac aagtgtaatt aatactgata
     5461 atttcctttt tttgttcatt ttttcttaac atcctttttt gttttgatta gttagatatt
     5521 ccttatctaa caagcctttt taggctatta aatgaagata aaccccaaaa aaatattgca
     5581 attatgacag tgcaatggta ctattattat acaataaatt ttgcgaaagg ggtaggtcta
     5641 aatcgcaaac aaaaagaaat tgaacctaga caaatacaac ttgtataccg caagggaagc
     5701 aagtttgatt ttgggtaaag gtagaaatta tgttacaaac ataatcaact attatccaaa
     5761 tcgtttgcct aaaggttcgt tcaagaaagt gggtgacaaa acctatatta taacggaaga
     5821 aggaatagaa gcattgcgat cacaaacaga aaataaaaac aaaaagaagg aatgaatatg
     5881 aagagaaatt cgcttggtat ggttttattg gcatcagctg tacttgcggg gttatcagta
     5941 ccagtaacct cacatgctga tgaatggact gcaaacacac cggatcaaat ccaaattaca
     6001 acaggacaat caagttatgc gttcaaaaaa ggagatacta tttggtcaac tgtagagaag
     6061 cttaaaagct taggttacaa gactactgtt gctgagtttg ccgaattgaa caatgttgat
     6121 ttggcaaaag gagaacaacg gactatcccg attggacgaa ctttcaacat cagtaaagat
     6181 acattaacag ttaaggaagc agatggcgaa gtagttagtc aaactgttca aaaagataaa
     6241 cctgatccta ttgaaaatac ggagcaagag aacaaaaaat ccgagactac aaaagaatcg
     6301 gaaccaacag caaacacttc tgataagaca gaaacaagtg cttcaagtgt tgatggagca
     6361 agttcatcta atacaaataa ttctacaagt gagaaagaag catcacaggg aacaagtgaa
     6421 agtagcacta cggaagctga tactggaaca tcaacaaata actcacaatc aacatcaaat
     6481 gatgcaaatg aatcaaccag tacatcttct agtgatcaat cacaaacagg ttcgagtgat
     6541 gaaactgaca gttctacttc accaagtaaa cccgaacaac caactaatgt tgtgctaggc
     6601 gttccattta ttagtcaagg taacacaatg ctttgcgaag gaacatcttt gctagaagcg
     6661 ttacattata aaggtgttgc aacaaatcaa gatttaatga cttttgttaa atcaatgcca
     6721 ctatctcctg acaataatcc aaataatggt ttttctggtg aatggagaca caatgtggat
     6781 ggaacttatc aaggtatgaa tccggctcct gtagtttcat ggggtgttgc taatggcgga
     6841 aatgttgtag atatttctgg ttcatcggta caaggattaa aagatgagat tgtaaatgga
     6901 aatcctgtag tagcgtgggt aacttatgcc tttgaacctg ctcaacataa accaatgttt
     6961 tggggcgatg ctatttggaa tcgacatgta gtagccgttg atggatttaa agacggtttt
     7021 taccatgttg tcgatcctgt gtttggggca agttggatta atgcaggatc atttgaaaca
     7081 gcatataacg tatctcaatt agcggtttcg gtacgttaat aaaagatatc gtttatatac
     7141 taagtagtta tatagtcttt atttgtcctt gcttaactac ttagaaaact tgttgctcat
     7201 ggtgggtaga actaaaaggg gtgatgctat gaatactaga gcgttgaaac agcttgcttt
     7261 caacgaaaaa aatgattaat aggtgtggtg attttaactt aggaatgggc gcgaaggtca
     7321 ttaccacacc gacatatcgt ggctcatttt cttcgcctaa ctggtataaa gaaggagtaa
     7381 cgagagatga agcatgaaaa aacgataatt ttaaaatcaa aaataattta ttgttttgcg
     7441 actttagtaa tgatgttatc tacgcttata atgccttttt cagcaataag agctgaagca
     7501 gatgtcacaa aaccgacaag tgcaaaattt atagaaactg gcgtaaaaac agacggttat
     7561 attagggtta atatgccgaa tcatccaaat gaatggatga tttcaagtca atttaaagat
     7621 tctcatggaa atattggata ttgtatggat tcagaattgc catccccaac aggaagtgga
     7681 gccggaagct tgaagtacaa gggagctggc agtgatgagt tctatcgtat gtttaaaggt
     7741 ggttttccat caaaaacagc aaaggaactg ggtgcaggaa atgatacaga agcatggtat
     7801 gcaacacaat tagtttcatg ggttttagcc ggaaacttta aagtttcaca gattgtatgg
     7861 tcacatccaa accataccgc cgcagaaaca gctcgtgtaa aaaaagcatt tgaaaaaatt
     7921 tatgactatg ctaagaatgg aaaagatacg cccaacactg agttttctat cacagcttct
     7981 aagacagctg acgaaggaaa atatcacact ttcacatata aaacagcatc aaataagact
     8041 ggtaatgcga agctcacttt tacatcagca aaaccagctg gtatgaagat ttatgatgca
     8101 gatggtaaag aaattactaa caacactgtg aaattaaatt caagttttac tattaaagta
     8161 cctgtgacta caccaagtgg tactttgtca tttaaaggta ctgctaatgt tagcacaacc
     8221 aatcctttta cttttgatgg acgaggtgtg tatcaagatg ccgtagttat gatcactact
     8281 tcagaaacaa aggactctaa atctttgagt gctaaatgga caagagctgt tggtcaggta
     8341 acagttaaga aagttgatca agatggaaat gctttaaaag gagcggagtt tactctaact
     8401 gacaataaca atgctaaatc tgttaagaca accggagatg atgggttagc tacgtttgat
     8461 ttgcaacaag gcttaacata cagcttagaa gaaacaaaag ttcctgaagg ttatcaaggt
     8521 tcatttaaaa aggatggtat tactgttaat accgatggac aagaatttag ttacactgct
     8581 acgaacacaa ttaaaaaagg taaagtaaaa gttaaaaagg ttgaccaaga tggaaaacca
     8641 ttagctaatg ctgaatttac cttgacggat gattcatccc aaacacaagc agaaaaaaca
     8701 gatgaaaacg gacttgcaag cttcgatatt gtagcaaaca agacctacag ccttgaagag
     8761 accggagtac caactggtta tacaggcgta ttcaagcaag aaggaattac cgttaaagat
     8821 gatggacaaa cttttgaata tacagctaca aacactttga aaaaaggttt agtaaaagtt
     8881 cataaagtcg atcaagatgg aaaacctttg gcaaatgcag agttcacttt aaccgatgaa
     8941 aaaggtgcaa cacaagtaga aaaaacagat gaaaatggac ttgcaagctt caatattgta
     9001 gcaaacaaga cgtatagcct agaagaaacc ggagtaccag taggttacac aggtgcattt
     9061 aagcaagagg gtatcaccgt taaagacgat gggcaaactt ttgaatatac agctacaaat
     9121 aatttgaaaa aaggtgtagt aaaagttcat aaagtcgatc aagatggaaa acctttagca
     9181 gatgcagaat tcaccttaac ggatgaaaaa ggtgtaaaag aagttaaaaa gacggatgca
     9241 actggtctgg ctacgttcaa cattattgcg aacaaaactt ataacttaga agaaacaaaa
     9301 gtaccaacag gttacacagg cgcattcaaa caagaaggaa ttaccgtcaa agatgatgga
     9361 caagtctttg aatatactgc taaaaacact ttgaaaaaag gtgtgatcaa agttcataaa
     9421 gtggatgatc aagggaaagc attagcaaac gcagaattca ctttgacaga cgacaaaggt
     9481 gtaaaagttg tacaaaaaac ggacagcaat ggcttggcaa catttaatat tgttgccaac
     9541 aaaacgtaca aaatcgaaga aacacaaaac cctaatggtt atacagggaa atggtctaaa
     9601 gaaggcatca ccgttgctga cgatggacaa acctttgaat atacagtaat taatcacctt
     9661 gtacctgaaa cagtaagaac aggcggaatc ggtacatcag ccttccttct tgtatttcta
     9721 attgcaggat tgagtatggt gctactgtat tatcgagaac agcacaaagc aaaataaaaa
     9781 agtgttaatg gaaaagaagt aaatatgaaa aaagaaggct gacgtatgaa atcaaaaaaa
     9841 attttttcaa atttaatgct atttggtact ttgctaggag tgggattggg cgcaagctca
     9901 atctcttcta gccttggact aggggggcaa gtagcatatg cacaacaaaa ctataattat
     9961 caaggaactg gaacgattca tatccataaa gtaacaaata ctaatgggga tgggaaccct
    10021 gtctcagata ttgataacaa agggaaagaa atagctttac caaatggtgt aaaacctctt
    10081 gaaggcgttg agttctctta tgtgaaactt actaaagatg aagctaaatc cttgggatgg
    10141 acggatagtg atactacaaa aggtcaacat attgatgatg ctaaagcacg attattacta
    10201 aacaaaaaat acagtgaagt aagtagaatg aagactgatt cacaagggaa tatagacata
    10261 cctatacaag taaatacaga cgatttaaca gataacttgt atttgattgt tgaaacagga
    10321 acagtcaaag gtgtagttga agaagcgtat cctatgattg ttaacgtacc tgaactaaac
    10381 ggatcagatg atcaatactt taattatgat gtacatgtct atcctaaaaa ctatttccaa
    10441 ttaaactcag cagagcttga taaaaaagac gaagatggaa aggctcttgc cggaactgtt
    10501 tttgaattgc gaaaagatga tggatctgat caggttgttg gaacttataa aacagacagc
    10561 acaggtaaat taactgtgga tggtttggaa ttaggtgact attatttcct agaaaccgat
    10621 ttgggaacag atgctaatag aacaacctat ttactagatg gtaaaaaatt caaatttagc
    10681 gttaaacgtg gggagactgc taaagtagtt cttgatgtta ccaactacaa acgacctgta
    10741 attactaagg taattgataa agaaaccgtt gatactggcg aagatgttca atatacattg
    10801 aaaccatcaa ttcctggtaa tattggtaat tacgaaaaat atgttgtcaa agatgttcta
    10861 ggtgcagagt taacttatga ttcatcagaa gtgatcgaag gaatgacttt agatactgat
    10921 tacagtatcg aacgtgacgg acaaacgaca aactatgtct ttactgaatc aggtatggat
    10981 aagcttcaga aacaatttga tgcaggggat cgcttcttta caattttgat gaacgctcat
    11041 actaatagta aagctaaaat ggatacacca cttaagaatg atgtttcaac gatctacaac
    11101 aatggatatg aacccgaaga cgaaacacca gttgatcacg cttatacaat cattggtgat
    11161 caccaattca ttaaaacaga cggagataca agcaaagcat tgagtggagc aaaatttgta
    11221 gtgaagaaca aaattgatcc gaataaagtt acttacctaa aacaagatcc aacaacgaaa
    11281 gctgttactt ggattgcaga taaatctaaa gctactgaat tcgtttccgg atcagacggt
    11341 aagttccaaa ttgaaggttt gcaatatacc tatgatgtag attttgataa acaaggtaat
    11401 ccaacaatca cagataaaaa aatcaatgat tatgctcttg aagaagttgt tgctccttca
    11461 ggatatacac caactagaaa cgatgttcca tttgtggtat cgaatgatga tgcttctagc
    11521 gaagtaacag acatcaagaa ccttaaaaca cctgaaacag ttcgtacagg tggaattgga
    11581 acagtagtat ttctagtggt tgcaggtgtg gcaggaacag ggctatatgt tgtctacaaa
    11641 cgagaacaac acaaagctta atgaaataaa attccctaga gtgtttttgg ctctagggtt
    11701 tctttttaaa aagtgtgtga aaagtaggta tgagcatgaa aaagaagaat atcaagttga
    11761 tcatcttaaa aattgggatg ttgctatgtt taggagtagg tctatatcct gtaattactc
    11821 aggcttatac ctactaccaa actcaacaaa cactaaactc ctataaggaa aaatatgatg
    11881 aagcttctga aaaggagaat gaagaagtct tcaagagata ttcagaagtt aataggaaac
    11941 gaaaagacac agaacaaacc tcagtcacga ttcctgaaga taataacaat attccgtcaa
    12001 atggaaatct tcagaaggat aaattaggtg aattagtggg tgaaattctt ataccaaaga
    12061 ttaatgtgga tttgccaatt tatgatggtg taaacgattt ccaactatca gcaggagcag
    12121 gaatattaac cggaactgat tatccaactg gtgggattgg aaaccattct gttatatcag
    12181 ctcattcagg cattcctaat aaaaaatatt ttacagattt agagttgctc aaacttggtg
    12241 ataaattttt tattgatatt tcagggaaaa aacttgctta tcaagtagat cggataaaaa
    12301 caattctacc aaccgaattt gaggatttgc ttcctgtggc aggaaaagat tatgtaacac
    12361 ttatgacatg tacacctgtt ggagataatt ctcataggtt actcgttaga ggggttagca
    12421 taccttacca aaaaagtatt gttgagaaag aaaagaagaa agaagattta aaggtaaagg
    12481 cgcagatgaa ctggtatcct atcgtagttg taggggtttt attaataata gctgtgatag
    12541 tcattattaa aaaaatagta gcaaagagac gaggaatgaa aaatgaagaa taaaatacta
    12601 ctttttttga ctcttatttg cactttggtt ttaacaattt atcctgcaaa tcagctctat
    12661 catcaattca aaacaaaaga gaatgtggcg gaaatccaag agaaaattga gaaaaaacaa
    12721 actccttcaa aagaggaagt ggcaaaaaaa gtcgaacaaa taaaatcgaa cagcggtaac
    12781 caaggattag acaatactca gaaaacagat aaaggagtag acaatactca ggaaacagac
    12841 ggtggatcat ttgatattcg tgaagctgaa ggaaaggtca atccatacac aggagatgtt
    12901 ttttcaaaaa atcaaacgaa agattttgaa ccatctacct attcaggagc ggttggttat
    12961 gtctatattc caaaaataaa tgtgctagag cttttgtttc taggcgcttc acaggagcat
    13021 ttatatagag gtgtggcaca aatgagtgct actagtctac ctttatcagg taaaggtcat
    13081 caaacagtaa tagcaggtca tcgtggcggt tatacgtcac cacaatttct ttatgttgat
    13141 caattagtag ccggagacca catttatata gattatcttg gtgaaaaagc agtttatacg
    13201 gttttccgaa cagacgttat tagaaattac ggtcagaatt caacactgac tgatattcct
    13261 gatttagata tgttaacact ttatacttgt catccatatc ctcagaacta cgatcgttta
    13321 ttagttagaa gtctaagaga tgaaaaagca aaatacatca cgccacaagt aacagttggc
    13381 aataccgcat acgctgatgc cattgatagt gaatccgttt cagataagcc agaaactgtt
    13441 acatcttcta atgatttatt tgaggatgtt gatcgtgcca acaaggaatt agatggaaat
    13501 aatgatctta atattccgga acaaggtagc gtagagatgc aagacagaac gtttgctgaa
    13561 aaggcaagtt caatatttac aggaattacc aataaacatc agaaaatgct tttagtcatt
    13621 tggactgtat taggagcatt agcaagtcta aaggttctta ttcttttaat caaagagttt
    13681 agaaaagcat aatgaagatt ataaaaattg atagagtaca gtaaaaaggg attgatacta
    13741 ttattgatct cttttttatt ttgaatagag ctagattcac ttttaaataa aataaaaact
    13801 gatcttaatt ccaaagaaaa agtcaagaaa aaaatacaac aaattttttt atcagaatat
    13861 ttatcagaat attattagaa aagataaact caaaagctta atttaatagg gttattggtt
    13921 gtatttagaa ataatgtatg gaaaagctat aaagcaatag ataagatgta gaaaatctta
    13981 tttgtgaatg aaaaacaagt catcgtaaaa tattaatagg aggtgtagta atggtggaaa
    14041 tatcaaaaac agctttagac tcattagcta aactatttag tagcaggttt gatggtgcag
    14101 aatttgttga atatctaagc caaaacgaaa gtggaaaaac tctgatcgaa acactgaaaa
    14161 aagactattt gaatgaaaat gactttccga catggacgaa tattgaagat gctacatggg
    14221 atgtagtgtg tacgatatat tctccttggg taacgattgc tgattttgtt ttagataaaa
    14281 ttggagggga tacataccct gatgccaaat ctcttgctac aagtgacttt aaaacgttct
    14341 atggttatct aatgggtcta ataggcaatg gaatagattt gaaagttacg ccaaaacaaa
    14401 cacctgaaga aattgaaaaa gaaaaaggag aggttcaaat ggcttatgca gatgacagag
    14461 aaaaagtaaa attattggaa ggtaaattat ctgaagaaga acttggaaaa atggtgcaag
    14521 aaatcaatga agacagcaac tattttcctg aattgaaggt atatcaaaac gacgaagatt
    14581 tctttaatga aatgtttagt aatagtccat gggaagcagt gagtaaaaca aatttaggag
    14641 aatggaactt tcaagatgat ctagttgcat ttagcaatga tgggtattta cgatcatatt
    14701 cagaaagtga gtatagagct gaattaaaaa cgtatgaaac ggatattctg atgagctatt
    14761 taaatctatt agagagtggt cagggagagt taagagataa agttgaagaa ctattaaaag
    14821 aagagctata gggattattt caacataaac acaagcagaa aactaccaaa caaaacgtac
    14881 catgcaaaaa gccggttaaa ccggcttttt gctctattat gagaggagat caaaccatga
    14941 gtgcaacaac cgatatggct ttagaagcag taataaaaga taatacagga aatgtcgaaa
    15001 atgtagaaga ctttagcgat attgcaatca atctttcagc atggatttca gctgtaggag
    15061 aagaaaatat cgaatttgag atcaagaata tggatgactt aaaagcaaaa tttcctttta
    15121 tcgcatcgaa actaagtcaa tttgccgagg agaaatcgtg gaatgaatta gttagttttt
    15181 ccgaaaaaat ttatgatatt acgcaagaag atgagtgtgt ttcatgggaa atgaataatc
    15241 aatacaagaa aattgatgct ttcgtcaaat cacgtataga aggaacagct tttgagtttg
    15301 aaggtataga taccgcagat aattggttaa ataaagaatc ctttattaaa agtatagcgg
    15361 atgatcttga aaaacaatgg tctaaggaag actatgcaga gtacctaaaa acagaaagag
    15421 atacagaggt gaaagaaaaa ctcactgagg tatgtttgga tgaacatatt gatcccaata
    15481 tcgtaaaaca agaagactat gaagtggtta ttgataaagt tcctactgat tttgaggaag
    15541 ttgtaaagta tatagttgaa gaagcattat cgcaatactc ttcagatgag gaagtggacg
    15601 aagaaactgt tcgtaccaga gtttggcaac tggtttttga tgacaaagat tataaatatt
    15661 tgagtgatca aaactcggtt aattggagtg tacgattatt aattgatcaa aatgatattg
    15721 aataaaatag agctgaaaag ctctattttt tttgataaaa aaactagcta tttttttaaa
    15781 aataatttgg ttcaaaattt atttttttaa cttgataatt ttttgccatc cttttttaaa
    15841 ttggctatat atcaaggttt atagtgggga taaataaaaa tacatttgac aaaatttaca
    15901 atatagttta ttatatattt atcttattat gataacacaa taaaaggaat gatggtatga
    15961 aaaagaagaa gtttagtcga tcgttgttag gaattttagt tttagttgcg gttggtctta
    16021 gtggcaaggg ttactttcag aataacagca ctcaggaaca tgattttcag atcaaaagta
    16081 gtgttcaggt aggtaataaa gcagacagtg aatcaaaaga atttttaggg atacgttatg
    16141 atccaaacaa atatccaaat aatatcgtaa acattaatga tgggaaatat acactgacaa
    16201 aagaagacca tgaagcatta tcagaaaaag gcagtcaaac cttttgggta aattatgcag
    16261 atttagatca acttggaaga gcaggagatg ttgaagcgtt gatcacctat gatgctataa
    16321 attctcactc atctaaaaat gtggaacgtc catctttcaa aagttctgtc catattgcag
    16381 gagaatacaa ggatggagta tttgatcaag agagagatac ttgggtcggc aaagattcaa
    16441 acaatgaaat cactcagcta gaaggatata aaggttatct ctataacaaa agtcattcat
    16501 tagcatggtc gctaggagga gatatggagg tacacaattt aacacttgga acgagagcgc
    16561 aaaatgttgg gaaaaatgat cagaatggag ggatggctta ctcagaaacg attgtacgtg
    16621 atgcaatcta taaaaataag aatgaaaaag tattgtataa agttgatcca atttataaag
    16681 ggaatgaatt ggttcctcgt ggttctcatg tcgaagcttt ctcagttaac gataatggaa
    16741 aaacggtaaa ccttaatgtt tatgtattta acaatcaaaa aggaatcaac atcaactatc
    16801 aaaatggtac ttgggtaaga gattagaaat tgagaaggtg aagaattgga aacaataagc
    16861 atcaatggat tagataaatc agttgcaaat cgaataaaaa aaggaaatgg ctactatctt
    16921 ctgaagaagg gtaaatattc agttgctaga gagttagaaa caatgagtat cgtgtatgta
    16981 gctaagaatc attttgacgt agaacaggct atttttgatc cagattctga aatagggaaa
    17041 tgtgttgaat tatcagagaa aactgttagt acaaaagggg cgatgatcat tgagagagaa
    17101 atgttagacg aaatcagagt aatcattcct aaagatacgc agatgtgtga ggacagcata
    17161 agtgacgatg aaccaaaatc agaaagaaaa taaggagata ttcagatgag cgaaaaatcc
    17221 attgaattaa aggaatataa gcgaagcgat ggagaaaaat attatcctgt agccagtttt
    17281 tctaaatcag tatatctggt tggacgtgca ttggtagttg gtgcaattgg catatatatt
    17341 ttgcattggg taacaaaggt tgtacaactg cttgtagata ccttagtaag tattcttgtc
    17401 tcgttaaatg atgttaacgg aacagatgat ttaggattaa aagaaatgtt taaagagtta
    17461 ctcaatgcgt tttttcccaa ttatttaagt tattcatata atgtacaaag cttttttgca
    17521 aagctacact tggtagacat ctctatttta gtattagaag caggaacggt attctttgtt
    17581 ggatatttgg ttactttgat gttcaggcat cactatggag aacaaagacc attttttgat
    17641 gatatggaaa gtagaaggtt gaaaagagaa gctctagaag ctatggatgt gaagtatgat
    17701 ccaaacaaga ttataggaga tgaaacgaac aacaaaaaaa gaaaacgaaa caaaacgcaa
    17761 ttagaatccg atgtgctatc aacgcttaaa aatatcaaaa tttctgttca tacaagacgt
    17821 gatctaagtg tcaatgaaga tattcgagaa tatatcgtaa aaatcccgca accaaaaggg
    17881 aagaaagtta gagaaacagt tttacgacaa attcaagatt tagggtcgat cctgacaaga
    17941 gcaacaagcg gacgagccaa atttggtgaa aaagaagatg aagtttcagg cgattatttc
    18001 attttccgag catcagtaaa tattgaaaga gaacgtaagg aaagagtaag taccaaaaac
    18061 aaagaaacgg aaagagcaac atcttcaatc gcagtttcaa attcacaatt ttcttttcca
    18121 atcaatctct ttgccgataa ttccaaaaat atccaaaacc aaaccgaaaa agctagaaaa
    18181 tatgccgaaa ctcaaaagca atctttagaa ctttatctat cttctgctga cgtacctgca
    18241 agtctaacag atatgcacgt tgggaataca tctattgaat atttgtttac tttatctcgt
    18301 accagaaaaa ttcaactaag ctcaatgaaa gaagaaattg aaacttactt gcatatatca
    18361 ggtgtaatcg tgcggatcaa cgcagggcgt attcaaatta ctctaccatt aggagaaaat
    18421 gatcgtattc caattgatgt accatcaatg atcaatagcg tgttttaaaa gacatcacaa
    18481 atagaaacag aaaatacaaa ctaccagttc acaaacagaa aagtaccgag aaaagtgagg
    18541 aatagaaatg gctatgaaat tattagagaa agtcaaaaag agagccgaaa aagctgaatc
    18601 tattttgcta gataaagaac ctaatataaa gcttaatgga aaattcagtg tcaatatgtt
    18661 gaacacaaag gctaaagacc cgacagatgc tatttttgga attaagcctg atggcacaat
    18721 tgctcatttt tgcatcagcg aaatgccaca catgttggtt gcaggaacga caggatcagg
    18781 gaaatcagta ttactgaatg aaattcttgt tacagcaatg tgtcactcaa cgcctgatga
    18841 attaaaaata ggaattgtag acccaaaaag ggttgaattt ggaagataca aaaaattgcc
    18901 gttcatgttg gctgatccaa ttacagatat ggatgaagct tatgattttt ttgaatatct
    18961 tgtgatcttg atgcatgaac gttacaaact gatggaaaaa gcagggttac aaaacatcac
    19021 gctatataac gaatatgccg aaaaaaatgg attagataga tttccctatg tgatcttatt
    19081 agttgatgaa tattcacagt tggttggaac acataaagaa gtagaaggtt tgattgttca
    19141 attaggacaa atggcaaggg cagcaggtat tcatgttatc ttagctacac aatctcctcg
    19201 atcaacagtt gtcactggta ttatcaaagc caattttccg tcacgtgttt gccttatggt
    19261 agcaagtgat ttagaaagta ggatcgtatt agatgaaggc ggtggagaaa gcctaagtcc
    19321 aaagggagat atgattatta agctcgttaa tggaagtatg gttagagcgc agggagctta
    19381 catcagtaac aaagaaattg aaacaatttt caatcacctt agaaatacta tgccagaacc
    19441 agaatatgtt gattaccgag caatcgtagc tgaatctcgt ggtgagggtg gagaagacga
    19501 ggattttggt ggaattggta aagctcctgt aagaaaatct gctaaaagaa atttgaacac
    19561 acctttagca aattcatcat ctaaagagcc atcaattgaa aagaagagta cgtttgctct
    19621 aaaacttgaa aaaaacaaag aagaaaaaga aaaacaagga gctaaaacac tagaaaaact
    19681 tcaagccgaa aaattagaaa aagttttaaa agcattagat aaagctgatt ctgaaccgca
    19741 aacctcaaac aacacgaata atgaacagcc gatttcatct aaaactaaac cgaaaactac
    19801 tgatccgaaa gtttcagtaa acgtgaccat cacaaccgat aagtctgaga agaaacaagt
    19861 aaacggaacg acttcaccta tcatgcaaaa aatgttgaaa gccaaaaaac aggtgcataa
    19921 atgatcattg tcagttcttt tttgaggtgg cttacgccat ataaatatac agatgaagta
    19981 gttgtgaaac gaacgaagaa aagagataaa aaagcggtac taactcctaa acagatgctt
    20041 gatcatggca ttttaggggt gaaggtttta gaagtgaata aaccgtttga atcaaatagt
    20101 gcaagagaaa agttgcttgg agctgaagcc gatgtcgcat atttttacca tgacaaaagc
    20161 aagcatcaaa tagctattaa attcaatcca aaatcggcat ggcggtatca agcaagagat
    20221 gacttgaaac gtaggaaact tagaccaatg ctctatccag aaggaaaaga agcatttgat
    20281 agaacccaca tcgttcccat tggttttcat ggtacagaat cagataaccg cctattagta
    20341 ggttggaaca gcaatcaaaa tagagttcag atcaataaat ttgagaagaa agtaaggaaa
    20401 ttcaacgatc gaaaaacgat cctatggttt gcagatattt gtaaacagcc ggatggaaca
    20461 gcaaagtggg aagcggtgat atttgacgaa aatggtaaaa aagtaatgtc tgaaacatgg
    20521 catgataaag ctaaatttgt ttggcagtag cagtagaggt gaaaaagatt gctagaactt
    20581 tttgaagcaa atagaacagg ctttaatcaa aagttagaag tgggagaaac tatagaatat
    20641 aaagataaaa actatattat tatcggaatc tataacacga gaattaacag aataggtgat
    20701 ccacgtataa ccagtgactt ggtttgtcaa tctgttaact attcacctga tctgacaagc
    20761 aggtataaga aatacgtgtt attagaatat agacacaaga ttactgatga aattggtgta
    20821 aacaacacac gaaacatatt taagatcgga acagtgattc cttatagtaa caacgaagaa
    20881 aaaatatttt atcagattta cggaatagaa aagtttgaat atgaacatgt cgatctgcta
    20941 gtgacgtacc aaatgcgttt aatagaacct tggtcacagg cagaaattga taaagcagta
    21001 aaattgaacc gattatcaaa atttaacgta ttaggaaatg ccttttaaaa atcgcaatac
    21061 acaaacagaa aaataccaaa aaattagcaa tgaacgtagg taagaacatg acaataaata
    21121 ataataaccc taaaaaatat aatgctcttg tccaatgcta tatcgaaaac aaaattacaa
    21181 tgcaagaagt cgctgatgaa ttaggcaaat caaagagcct agtatcacac gattttaagc
    21241 acaatgtttt tccgaacccg attgagctga ggaaaaaatt tcaaaagaga gtttatcaag
    21301 aaataggtaa gtggattgat gaagggaatg caattgaccc tttaattgaa aaatatcctt
    21361 ttttaaaaag caaaaataaa attcaaaggt taattcgtca aggcgatatt caagctaaag
    21421 aacctgtttt gacaaccaat gatttggcta aaataaatct tcataaacaa attattgaag
    21481 actttgaaaa aaatctttca acaactgaaa ttttaaaaaa atacaatatt ccgagaggaa
    21541 agctaacatc gactcttttt agagaatatg gagaatcggc agttactaaa gcaaaggaag
    21601 tagcgaatga gagaaaaaaa caaaaatagt agtcgatacc tagtagcgac tgtagttttt
    21661 gtttgtgtat tgattttcct gtttgatttt ttgcagattg gaaaaagcat aattggaaat
    21721 acattgagtg caaaggaaat ggttgcttta ggtggtttgt atgaaggaca cttgttcccg
    21781 aatatattca tttctatcgt ttctcatatt aacttgtacc atttaatttc taatttattg
    21841 ttcttagtag ttcttgaaga agcactgaat gatcagtatg agtggttaac attattgatc
    21901 gttttaatca tatcaggatt tctaggaagc ttagttcctt ttctgctaca cagcaacatc
    21961 gtaagtatgg ggcttagtgg aggtattttt ggatggattg ctttatttgc gattgcatac
    22021 atccgttttg atagcaagaa acatttatta ctaagtgttt ttgtattatt gataatcggc
    22081 attttactgg tatctacttt tgaatcaggc gagacaaacg tcttgtcaca tgtaatcgga
    22141 tttgtctgtg ggatgatctt gggatttatt ctaaagaacg tttcattgat caaacaaaag
    22201 aattaaaagg gagtgctaaa aatgccaaaa aaaaaagaaa agaaaattcc agtgtttttg
    22261 tctgtctcag aaatgttatc acttcaaata ctgttaagaa aaaagattga aacaatagac
    22321 gaagaagtta gaaataaaag ggctatgtac gaacaacgtg aagcgctaag agaacgaatc
    22381 cactattttg atgatataca cactaaaatc tatgaagcac gtacaaatca cgaagacgaa
    22441 agcaaattag gattagctca acaacgaata tttgaatggc tgaatacgaa tactgagcaa
    22501 gaaccgttaa gagcaattag taatcttttt gaatttggaa cgaagcattg gtttaacgct
    22561 gtagaaaagg atgctgactt acaagaagcg ttcgatacac taagcgagga tagcaaatcc
    22621 atgcgagact tattgatcac ttatctaaat gagcatgaat ttgaacagac gaaagttaaa
    22681 aagaaaaata aaaaatagaa agttgtgatg tatcatgctg tttaccttat taggttattt
    22741 atgtttagtt cttgcttttt ttggagcaat tgctatattt gtcggtcagt ttcaagaaaa
    22801 taatccgata atgcgtaaca taggcattgg aattgttatc atatccttga ttgtatttgg
    22861 tgccacaaag atttttggga gtaatgatca gcagtttggt atttctggag ttgataatag
    22921 ttatgctcca gattcattgg gaagagtagt gatagatgga acgtttgaga atgtaacgag
    22981 ctacaaaaat gtttcattga cagtgaaaga agatgacaag aaagcatcag atgacgttct
    23041 cgctacgatc aagccgtctc aatcacagtt tgaagtgttt tatacagtca aagagaataa
    23101 ccctgtacgc aagctaacat tagtttttaa cgttgacgga gaagaatact ataaagatgt
    23161 aaaaatcaat aaagcatcaa cgaaaaagat tgtcgaatca gcgaaaaaag aagtagctac
    23221 agaagaaagt acaactgaat caagttctac tgtcgaagtt gaactaccag ataaaattga
    23281 ttttgatcct gaaaaagtca aatcgtttga atctgtttct ggttctatgg cagatcggtt
    23341 taacactgaa gttttcaaag ctatcaaaac tgttaaattg gatactgaaa agcaaaggat
    23401 taatgttacc ttattcaatg cagttgaatc tatggatcag gataaaaaag ttacggttgc
    23461 aagagaacta gggaaaagag taattggatt gtatcgaggt acggcaaaac cagattcaga
    23521 cggttcagat ttgccagttc acgtagttta tgaagacggt aaagtttttg gagactctac
    23581 agcaaatgaa gcactaacga ttaaattagc taaatagccg gaaaattata ataaacaagt
    23641 ggtgtcacac caccatcgca taggtgtgac accttggaga tgattactga tggtagtaaa
    23701 taaagaaaaa gtaagaaagt atgttacttt aaaaatagct tctgttgaat atttagaaaa
    23761 gcgagctaaa gaagaagaaa aaacaagagg taagaaggta acagtttcag agttgttaga
    23821 agaattaatt gagagtgacc aaacaataag acgtgcattt accaataggt ctgttaaaag
    23881 ttagggtgat actatcttta atttcaacca aatttctaat atatgatgaa tagtttcctt
    23941 gtacaaaata tctaattaag agataaaagt taggaggatt cattcaaaat gattatccaa
    24001 aagtttgact ggaaaatgaa attaggaatt agtcttgtaa tattgggatt tattattttt
    24061 ttggggataa aagcacaaaa aaatcaatct acttttgaaa ctatgtcttc tcaaattatt
    24121 gaggaatgtt ccaaaattta ttattgtgag aacaaaactg aagaatctaa tggcttttat
    24181 tatatcgatc aaccacatga tgttgttata aaaatggagt cacatgaagt aaacgaatgg
    24241 attcaaagaa gtactaatgt atcaaaaaag ttagaaagat attctcaaac gtttttaagt
    24301 aaagtcattt tttcaaattg caaaaatgaa gaaacgtctc aaagagtagc
//
LOCUS       DAEGIQ010000033        24333 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000033 DAEGIQ010000000
VERSION     DAEGIQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.033"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..835)
                     /locus_tag="KMT45_001215"
     CDS             complement(<1..835)
                     /locus_tag="KMT45_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBE7004686.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YF"
     gene            complement(1059..2999)
                     /gene="thrS"
                     /locus_tag="KMT45_001216"
     CDS             complement(1059..2999)
                     /gene="thrS"
                     /locus_tag="KMT45_001216"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBE7004687.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3336..3515)
                     /locus_tag="KMT45_001217"
     CDS             complement(3336..3515)
                     /locus_tag="KMT45_001217"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBE7004688.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3562..5385)
                     /gene="cadA"
                     /locus_tag="KMT45_001218"
     CDS             complement(3562..5385)
                     /gene="cadA"
                     /locus_tag="KMT45_001218"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBE7004689.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5663..7942
                     /locus_tag="KMT45_001219"
     CDS             5663..7942
                     /locus_tag="KMT45_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBE7004690.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8035..8682)
                     /locus_tag="KMT45_001220"
     CDS             complement(8035..8682)
                     /locus_tag="KMT45_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004691.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8861..9580)
                     /locus_tag="KMT45_001221"
     CDS             complement(8861..9580)
                     /locus_tag="KMT45_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7004692.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9711..10862)
                     /locus_tag="KMT45_001222"
     CDS             complement(9711..10862)
                     /locus_tag="KMT45_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBE7004693.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11079..11819)
                     /locus_tag="KMT45_001223"
     CDS             complement(11079..11819)
                     /locus_tag="KMT45_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE7004694.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11823..12146)
                     /gene="rsfS"
                     /locus_tag="KMT45_001224"
     CDS             complement(11823..12146)
                     /gene="rsfS"
                     /locus_tag="KMT45_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBE7004695.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12203..12796)
                     /gene="yqeK"
                     /locus_tag="KMT45_001225"
     CDS             complement(12203..12796)
                     /gene="yqeK"
                     /locus_tag="KMT45_001225"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBE7004696.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12780..13430)
                     /locus_tag="KMT45_001226"
     CDS             complement(12780..13430)
                     /locus_tag="KMT45_001226"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBE7004697.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13440..13751)
                     /gene="yhbY"
                     /locus_tag="KMT45_001227"
     CDS             complement(13440..13751)
                     /gene="yhbY"
                     /locus_tag="KMT45_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBE7004698.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13767..14876)
                     /gene="yqeH"
                     /locus_tag="KMT45_001228"
     CDS             complement(13767..14876)
                     /gene="yqeH"
                     /locus_tag="KMT45_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBE7004699.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14873..15400)
                     /locus_tag="KMT45_001229"
     CDS             complement(14873..15400)
                     /locus_tag="KMT45_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBE7004700.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15580..16365)
                     /locus_tag="KMT45_001230"
     CDS             complement(15580..16365)
                     /locus_tag="KMT45_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBE7004701.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16358..17227)
                     /locus_tag="KMT45_001231"
     CDS             complement(16358..17227)
                     /locus_tag="KMT45_001231"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBE7004702.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17228..18607)
                     /locus_tag="KMT45_001232"
     CDS             complement(17228..18607)
                     /locus_tag="KMT45_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBE7004703.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18615..19034)
                     /gene="fabZ"
                     /locus_tag="KMT45_001233"
     CDS             complement(18615..19034)
                     /gene="fabZ"
                     /locus_tag="KMT45_001233"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBE7004704.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19059..19535)
                     /gene="accB"
                     /locus_tag="KMT45_001234"
     CDS             complement(19059..19535)
                     /gene="accB"
                     /locus_tag="KMT45_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBE7004705.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19538..20773)
                     /gene="fabF"
                     /locus_tag="KMT45_001235"
     CDS             complement(19538..20773)
                     /gene="fabF"
                     /locus_tag="KMT45_001235"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBE7004706.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20792..21529)
                     /gene="fabG"
                     /locus_tag="KMT45_001236"
     CDS             complement(20792..21529)
                     /gene="fabG"
                     /locus_tag="KMT45_001236"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBE7004707.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21541..22458)
                     /gene="fabD"
                     /locus_tag="KMT45_001237"
     CDS             complement(21541..22458)
                     /gene="fabD"
                     /locus_tag="KMT45_001237"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBE7004708.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22565..22789)
                     /locus_tag="KMT45_001238"
     CDS             complement(22565..22789)
                     /locus_tag="KMT45_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBE7004709.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22842..23804)
                     /locus_tag="KMT45_001239"
     CDS             complement(22842..23804)
                     /locus_tag="KMT45_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBE7004710.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23814..24254)
                     /locus_tag="KMT45_001240"
     CDS             complement(23814..24254)
                     /locus_tag="KMT45_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE7004711.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6651 a         5425 c         4242 g         8015 t
ORIGIN      
        1 taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt
       61 tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa agtgaattag
      121 ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt acatcggcta
      181 tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt
      241 tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa acaagccaaa
      301 gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata tctttttcaa
      361 aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact actaaagaag
      421 aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct acgacttttt
      481 ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt gcattgttac
      541 caagtttgtt tttgatagag taaaaggact catttgcttc tatatcacta aaaccttcat
      601 cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac
      661 cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct tctgctaatt
      721 cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg gccaactttt
      781 tttctgctga agaataatct tccatcatac tttcaatacg actaattacg ctcataagtt
      841 cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt attcacggtc
      901 gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttat cccatcaatc
      961 ccacagattc tattgagcat ctgtgtcttt gaggaatacc tgttatctat tgttcggatt
     1021 gctttctatc tactactttt ttacaggaaa tttacctatt agttttctct gctgtaattt
     1081 ttgacttctg cttccatagc atcaataaag atattcaaat cttctacagc tgtttcttta
     1141 cttccataac gacgtacgtt cactgttgca tcttccaatt ccttgtctcc aacaactaat
     1201 tggtaaggga tcttttgtgt ttgagaggca cgtatcttat agcccatttt ttcattacga
     1261 tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc ataagcataa
     1321 tcgccatgtg catcaacaga aacagggatg attgttgctt gtataggtgc caaccatgtt
     1381 ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa aacgttccat tgtagaaaca
     1441 attccgcggt ggataactac tggacgatgc gtattttccc cgtcttctcc aacatatgtc
     1501 aaatcaaagc gctctggcaa taagaagtct aattgtatag ttgataatgt ttcttctgta
     1561 ccaagagctg ttttgacttg aacatctagt tttggtccgt aaaaggctgc ctcgccttct
     1621 gcttcaaagt aatctagttc taattcatcc atagcggctt tcagcattgc ttgggctttt
     1681 tcccacattg cgtcatcatc aaaatattta tctgtattgt ttggatcgcg atagctcaag
     1741 cggaaacgat aatctgtaat attgaagtca gcatatactg ccaccatcaa ttcaagcgta
     1801 cgtttaaatt catctttgat ttgatcagga cgaacaaatg tatgaccatc attcaacgtc
     1861 atttctcgta cacgttgtaa acctgacaaa gcacctgatt tttcatagcg atgcatcatt
     1921 cctaattcag cgatacgaat tggtaattca cgataagaat ggatcgtgtt tttgtagacc
     1981 atcatgtgat gagggcagtt cattgggcga agaacaagca tttcgccatc tcccatatcc
     2041 attggcggga acatgtcttc ttgataatga tcccagtgtc cagatgtttt gtacaactca
     2101 acatctccca ttattggtgt atatacatgt tggtaaccta gactaacttc tttgtctacg
     2161 atataacgtt cgatcgtgcg tcggattgtt gccccttttg gaagccagaa tggcaaacca
     2221 gaacctactt ctggggaaac catgaacaag tcaagttctt ttcctagttt acgatggtct
     2281 ctttcttttg cttcttcacg gagacggata tattctttca aagctttctt gtcaaaaaag
     2341 gctgtcccgt atacacgttg catcatttga ttgtttgagt ttccacgcca ataagcacct
     2401 gccactgata ataatttaaa tacttgtata cggccagtgg atggaacatg tggtccacga
     2461 cacaaatcga tgaattcgcc ttgttgataa gcagtaatca cttcttcttc tggcaattca
     2521 ctaatcagtt ctactttgta cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa
     2581 agaacacggc gttcgatcgg cagattttct ttgacgattt tcatcatttc tgcttcgatt
     2641 gccggcaaat cttctgcagt tacttggttt tgtccattat ctgtatcgta gtaaaaccct
     2701 gaatcgatag ctggtccaac gccaaagtgg atatttggat agaggcgacg catggcttgg
     2761 gccatcaaat gagctgctga atgacggact aagccaagtg catcttcatg gtctggtgtg
     2821 atgatctcaa tacttgcatc ttcctcgatt ggacgagtca aatcaacgag ttttccgttc
     2881 actttaccag ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc aattgccaat
     2941 gttgttgttc cagactcaaa ttctttgact gcaccatctg gaaaagtaat ttttatcata
     3001 atagtttcct ccttgaaatg ttttgattga tcagatcatc aagctgaaaa acaaaaaaat
     3061 cctagcatct cttgaaaaga gatactagga cgaaaagttc gtggttccac ctaatgttca
     3121 aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt
     3181 tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt cagcctagct tttcctctct
     3241 tatcgaagac tctcaaattc agttggcttt ctcatcaatc aattatgtgt ttatttaacc
     3301 actaataaat agaaaaatca agtgggaaac taaacttatt ttttccattc cccatttaca
     3361 ccgtatgttc cttttttcat ctcattgatg atgacatgga tgtgttcttt tggtgcctgc
     3421 gtgtttttat gaacagcttc cgtgatatct ttcatcatat tttctaattg ttccggactg
     3481 cgaccttcta ctaattcgac atgtacgaat ggcataatgc tctctcctct ttctgatcgt
     3541 tcttttttca actcgattct atcatactat gtttgtcttg ggtactgaat caggaaaaat
     3601 ggctggatct ttttttgctc tcaaggcact caaaatagag accgtatcca cgacttcttg
     3661 caaaacagct ccgaataatg cggggattag tcccgtactt gcaattaaca tcaaaaaaat
     3721 acaaataaag atgccaatca gtacagactg tttcgctatt ctcatcgtat ctcttgaaat
     3781 cactactgct tttgctacac gcaagagatc atctttcaag atgaccgcat ctgcactttc
     3841 actagcagct gtagaaccat gagcccccat tgcaataccg atatctgcag cagcaagtgc
     3901 tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt tagggatact
     3961 ttctaaaatg gtcagtttat cttgaggcaa gctacgggca tgtacagaat ctaattggac
     4021 tttttctgca atcgtttgag caatggcaga tccatccccg gtgatcatca tcaaattttt
     4081 cacatcaagt tttcttagct ccttgattgt ctttgctgct tctgggcgaa tctgatccga
     4141 aaataggatc gttcctgcaa aagatcgatc aattgttaca tagattgctg tattggacgt
     4201 ttctatttct tcagctgaaa caaaagaggc acgtccaatc tttacttcat gattattgac
     4261 tactgcttgg attccttgac cggtcacttc tttcagttga ctaacaggat acagttggct
     4321 gtttttcacg ttgttcacta acgaacgagc taagatatgg ctagattcct gttctgcact
     4381 agctgcatac agaagtagct cttctttttt tattccattg actggctgaa tcgcctctac
     4441 ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc
     4501 gagcttttca atcgttgttc cagtttttac aacgatcccg tttttacttg aacggctcat
     4561 tcctgcaact aaagcaatgg gcgcagctag aatcagtgga caaggtgatg ccactaccag
     4621 tacctcagca aatcgtaaag ggtcttttga gaaccaccaa gaaataccac cgatcaaata
     4681 agcaatcaac gtgaaaggga ctgcataacg atcagctaaa cggacaaatc gggcaggctt
     4741 ttcttttgat tcttcgacta gtttaataat catttgatac tggctgtctg cggcacgttt
     4801 ttcagcgatc atttggatcg atccgtctcc atttaaagac cctgacataa tctgatctcc
     4861 aggtttcttg gtttccgggc gggactcacc tgtcaaagaa gattcatcga ccgaagattc
     4921 tcctttggtc actcgaccat ctgcgggtac aagttcgcca ggcttgacta gtaactggtc
     4981 atcaatattt atttcatcga ttactatatc ctgcaatttt ccttctttga atcgatgggc
     5041 aattcttggg gtgttatcta acaaagactg cagttcttta cccgctcgtt tattcgcgta
     5101 atcctctaga ctatctccgc ccgtaagcat gatcaatatc atcaaactag cccagtattc
     5161 tttgacggct aatgttgcaa ggattgcggt aatcgctaat acatctacgc catatcttcc
     5221 tgaacgtaac gtcttgatca tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa
     5281 tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca aattcactta ataaagctat
     5341 cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt tttctcctct
     5401 tttatgtgac ttagagaggt tagtaataaa tttccagctg cctctttcat cttaagcata
     5461 gtccttgact agcacttttt aaaacaatag gccttaattg aaaacaacta tcactatcag
     5521 cgtttccaaa attcatcagt tctacaaaca aaaatattca gcaaggatta ctttttcgat
     5581 tcatcagtta aaaagtacct tttttcgaaa aactatgcta tacttttaat tcgttattta
     5641 cggcggaagg agcaaacatg cgatgaatga gaaacaacta gaacaaacgc acgtggatga
     5701 aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat acaaaacaac aagaattgac
     5761 tcaggaaatg aatcattcga caaaagacac agctaatcac aagattcgag ctggttccaa
     5821 tgaagctttt tatgaatctg ctgttgaata tcgccaacat gagcaggaat tgcttttgaa
     5881 ataccatact cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag
     5941 tccttatttt gcccgtatcg actttaaaga agaccgtgaa acagaaacat tatatctagg
     6001 tatcgcttct ttaagagatc tggcagaaga aacaatcgtt attgactggc gcgcaccaat
     6061 tgccaatctt tattatgaag gtgagattgg tcccgcattt tatgaaactg aaacagatag
     6121 aatagatgtc gatctgcagt tgaaacgaca atttaaaatc caagaaggac gtattttatc
     6181 tatggtagat acgtcggaag taatcaatga tgactttttg ctagagatat tggatgaatc
     6241 atcaagcaac catatgaaaa acatcgtctc tacgatacaa aaagcacaaa atgcaatcat
     6301 tcgggacaca acaagtaaag taatgctgat tgaaggaatc gccggaagtg gaaagacatc
     6361 cgctttgctt caacgtattg cttttctgtt gtatcataat cgcaagtggc tcgaggctga
     6421 aaacgttctt ctattttcac ccaaccattt gttttctgat tacatctcga ctgtattgcc
     6481 ttcattaggt gaaagcggcg tgcctacaca gacattcaaa aattatttgg tgcagctgct
     6541 gccagagttt tctttactag atgaacagca acaagaaagt ggttttttgc taggggaaaa
     6601 agatccgata caagtgatga aaagcggatt gaccttagtc gatcagattg atcgctatat
     6661 ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat
     6721 cctatcaaag gaatcgattc gccaatggta taaagaaaca aatgaacaat tacctttaca
     6781 tcatagactt tctctattac aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga
     6841 cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa cagctccaag aactatacgc
     6901 aacagatcca aatcttgaat atacagaaca aaaggagcgt tcattacgta aaaaactggc
     6961 caaccaaatc gtacgtaaaa gattccgtcg aatccatcga gggatcatga attatcaatt
     7021 tgtcaatata acgaaacaat atctccactt tctacaaact gtgccacttt ctcttctaac
     7081 acgacataaa atcagcgaga ccaggtggca ggaacatttg acaactgttc gtacacactt
     7141 gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc tttttactga tgcgccgaac
     7201 atatccagtg acggttacac acaaagctcg ctatatcttt attgatgaaa tgcaggattt
     7261 cgcaccatca caagtggcat tgctgcaagc cttgttccca aaagcaaatt tgactttctg
     7321 tggagatcta aatcaaaatg tctttggaaa tgaaacgatc gttggatcat tggaagacct
     7381 tttcgtggac cgtgaagtca ctcgtttcca attaacgaca agctatcgct ccacaaaaga
     7441 aatcactgat tttgctaatc attttattgt gaaagaacat caagtagaaa caacagcaag
     7501 aaaaggagac cttcctttgc tcatccaagg aacaacagat gatatcaaat tgaattggtt
     7561 agcagatacc attcgaacga ctcagggaaa agcccggtat tggcgtacag caatcatagc
     7621 taaaacacaa gaagaatgcg aagctcttta tgctcaactg ccagacgagt tgaaacaaca
     7681 agtccagctg attttaaatg agagtgactt tatgaagcgt tccattattt tacttccagc
     7741 ctttttagca aaaggtctgg agtttgaccg agttttcttg tgggatgtag atgcagaaca
     7801 tttttcttct cagcaagaca aattgatcct ttacaccatg tgtacacgtg cgatgcatga
     7861 attgaccctt tttacctcca ataatgaaag tcctttgtta aacaatatgg atatttcatc
     7921 ttatgagaaa aaagtactgt aagtaaaaag acaactgcta tcattaatgt tagcagttgt
     7981 ctttttattt acaataataa ttgcagaaaa agtaattcaa tcatttaagg tcatttattc
     8041 tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt
     8101 cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc
     8161 gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa
     8221 attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc
     8281 actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt
     8341 ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc
     8401 ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt
     8461 ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata
     8521 ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg
     8581 cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct
     8641 taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct
     8701 tccacatact tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat
     8761 tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct
     8821 tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat
     8881 tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg
     8941 tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga
     9001 ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta
     9061 cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt
     9121 tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg
     9181 tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa
     9241 atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca
     9301 ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg
     9361 caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag
     9421 cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg
     9481 ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag
     9541 cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga
     9601 tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg
     9661 tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg
     9721 attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca
     9781 cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa
     9841 atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag tagtcgaaga
     9901 taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa
     9961 gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc
    10021 tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc
    10081 tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc
    10141 caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc
    10201 acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct
    10261 atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt
    10321 aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga
    10381 tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt
    10441 ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct
    10501 tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt
    10561 ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa
    10621 tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt
    10681 tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc tctttgcaaa
    10741 aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg tgccatttcc
    10801 acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc
    10861 atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca
    10921 aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat acttctgcga
    10981 cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag
    11041 atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag
    11101 aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact
    11161 ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct
    11221 tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag
    11281 gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca
    11341 aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac ttgataggtg
    11401 gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac
    11461 acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt
    11521 gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat
    11581 gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac
    11641 cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt accgcatgcc
    11701 agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc
    11761 tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata
    11821 tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag tttttccaga
    11881 ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc aagatcgatc
    11941 aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct
    12001 tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag
    12061 atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct
    12121 gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact atctatgatg
    12181 atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg
    12241 tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa
    12301 atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa
    12361 aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct
    12421 tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg gtgattccta
    12481 attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca
    12541 ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt
    12601 cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt
    12661 ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc
    12721 tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac
    12781 tatagtccat attcatacaa cccttcattt tgaatataat caattacttt atctggaaca
    12841 aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg
    12901 atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga
    12961 cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa
    13021 tattctacca tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc
    13081 aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca
    13141 atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg atgttttgca
    13201 tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg
    13261 tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga
    13321 ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct
    13381 tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct
    13441 tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat
    13501 aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact
    13561 tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct
    13621 cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg
    13681 atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct
    13741 cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca
    13801 ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg
    13861 gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat
    13921 gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat
    13981 tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac ataagcaata
    14041 aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt
    14101 tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc
    14161 aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca
    14221 atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac
    14281 tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc
    14341 gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct
    14401 tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca
    14461 tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt
    14521 tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca
    14581 ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca
    14641 ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc
    14701 gtcaatgaga catcttgggt ctcattataa tggcgtaatc tgaaacaacg ctgacaatag
    14761 acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg
    14821 ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga
    14881 ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt
    14941 cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga
    15001 tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca
    15061 ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta
    15121 aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac
    15181 gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa
    15241 tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta
    15301 aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga
    15361 tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa
    15421 aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa
    15481 aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa
    15541 taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc ggaacctctt
    15601 gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca atctttcaat
    15661 caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt
    15721 ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag taattttcat
    15781 caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg caaatccttc
    15841 tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg ctaaacctag
    15901 agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg gtacctttaa
    15961 atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct cttctgctcc
    16021 tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat tgaacttttc
    16081 cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag aaccaaaatt
    16141 tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg ttaccggttt
    16201 gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata aacgatcacc
    16261 atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc
    16321 tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct atccatcctt
    16381 tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg cggggaacaa
    16441 tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct tctggaagtt
    16501 cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag ctttggggtt
    16561 ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg gtcgtcgggt
    16621 ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca gcagcagaga
    16681 tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg
    16741 tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg gtgatctttt
    16801 ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact ccaataatga
    16861 agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt cctgttttat
    16921 cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct ttggttacaa
    16981 gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga cgctgccatg
    17041 caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg
    17101 tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga acagatggcg
    17161 agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt ttcttaaaca
    17221 gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa aattcttctt
    17281 gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc aaatccatct
    17341 gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct cgctgcatct
    17401 tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc attgaatcat
    17461 aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt cccatcccac
    17521 ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg aaagcaggat
    17581 tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct tcttgacaca
    17641 cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg attcctgtga
    17701 ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg aaataaaaat
    17761 cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg actgctttag
    17821 ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc actacagaag
    17881 gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat
    17941 gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga gcaggataaa
    18001 tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct tgctgagctg
    18061 aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct ttcccgccac
    18121 cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt tccaatgctt
    18181 cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt gcttcttgca
    18241 tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt ttaggaccaa
    18301 taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca gataaaaaac
    18361 caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt acttcctgaa
    18421 cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca tctgctaact
    18481 gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg atccctagtt
    18541 cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc aatactttag
    18601 aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac ttttttaccg
    18661 tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa tatctcaact
    18721 tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt gtccaatccg
    18781 ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct
    18841 tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg aaaatgtcct
    18901 tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc tccttcaacc
    18961 atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat ttcttttatt
    19021 tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg aatcctaaag
    19081 agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt gatcactccg
    19141 tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca gataacttct
    19201 cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc tggttgcaga
    19261 taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc cgattctttt
    19321 tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac tggctccgaa
    19381 actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt atttttattc
    19441 atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat
    19501 tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag cgtttcatgc
    19561 acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa gtaaaacttg
    19621 caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt ttttctacat
    19681 tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag gcttcgatcc
    19741 cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg taagtatctc
    19801 tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca ttggctggtg
    19861 tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca gcttcttcca
    19921 tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct gtcatatggt
    19981 aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca ccgcgagctt
    20041 gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc acaaaaccat
    20101 tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct gagagtgctg
    20161 ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt cctccagcaa
    20221 gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt gaattcgttc
    20281 cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca cgcaaagcaa
    20341 tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa actcgttttg
    20401 cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca ccgatcccag
    20461 atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa ccgctcatct
    20521 ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt cgtttgctat
    20581 cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct aaggtaatcc
    20641 ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc
    20701 tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag ccagtaatta
    20761 ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat ccgccatcga
    20821 cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct gtttgtgcaa
    20881 tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt tccttgactt
    20941 tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg gcattacatg
    21001 taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact acgccagctt
    21061 tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca ctagagagat
    21121 taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc gtcatattga
    21181 aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc attcgcatca
    21241 caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat ccaagctttt
    21301 cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca ccggaaactc
    21361 ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga atctctccgc
    21421 gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc gctttgccga
    21481 ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata acgttcctcc
    21541 ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca gaactggtat
    21601 tgttttatcg atttttttca taaatccgct taatactttt ccggggccga cttcgatgac
    21661 ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa caggttgcat
    21721 gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg ttgttgtgtt
    21781 gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct tcgccagttt
    21841 ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta gagggatcat
    21901 tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt tttcttcgcc
    21961 accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc cgtacttgct
    22021 agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg caaccatttt
    22081 cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta ctaacgcaac
    22141 tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc ctaaactcag
    22201 tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat acgcgatgct
    22261 gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa
    22321 gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg tctgctttac
    22381 aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt ggccactaaa
    22441 taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt gattcttcct
    22501 attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa cctaatttta
    22561 atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt
    22621 cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt tggaataaat
    22681 ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt tcttcagttt
    22741 cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata ataatatatt
    22801 cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc catgagaata
    22861 cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg ttttcctaag
    22921 gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc agcagatgta
    22981 ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac ttttcgggca
    23041 attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac tggtttgtct
    23101 ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg
    23161 ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg atttttattt
    23221 tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa tttttctttc
    23281 agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc gccaaacaac
    23341 acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc accaatcacc
    23401 agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc ataaacaaat
    23461 cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa tttcccttgt
    23521 acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa
    23581 tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac taaaatgcat
    23641 aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt ccttgacgaa
    23701 atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat aatcttttca
    23761 ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata tcctcactta
    23821 tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg ctgttcctcc
    23881 gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg atggacccga
    23941 aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc atcttcactg
    24001 cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt caaggtccca
    24061 acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta catcccgatt
    24121 gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt tttcagctca
    24181 gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt tactgttttt
    24241 aaatctggtt ccatgttcca ccgtccacta attagtttga ttatcaaatc ttttgatgtt
    24301 caaactatat cttaactttt ttcaaaatgc aac
//
LOCUS       DAEGIQ010000034        24122 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000034 DAEGIQ010000000
VERSION     DAEGIQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.034"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            70..552
                     /locus_tag="KMT45_001241"
     CDS             70..552
                     /locus_tag="KMT45_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004712.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            749..1639
                     /locus_tag="KMT45_001242"
     CDS             749..1639
                     /locus_tag="KMT45_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBE7004713.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1926..3200)
                     /locus_tag="KMT45_001243"
     CDS             complement(1926..3200)
                     /locus_tag="KMT45_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBE7004714.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            3473..5944
                     /locus_tag="KMT45_001244"
     CDS             3473..5944
                     /locus_tag="KMT45_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBE7004715.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6047..7783)
                     /locus_tag="KMT45_001245"
     CDS             complement(6047..7783)
                     /locus_tag="KMT45_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBE7004716.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7780..8484)
                     /locus_tag="KMT45_001246"
     CDS             complement(7780..8484)
                     /locus_tag="KMT45_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7004717.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8615..9118
                     /locus_tag="KMT45_001247"
     CDS             8615..9118
                     /locus_tag="KMT45_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBE7004718.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9078..9416
                     /locus_tag="KMT45_001248"
     CDS             9078..9416
                     /locus_tag="KMT45_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBE7004719.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9437..10855
                     /gene="ffh"
                     /locus_tag="KMT45_001249"
     CDS             9437..10855
                     /gene="ffh"
                     /locus_tag="KMT45_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBE7004720.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10967..11545
                     /locus_tag="KMT45_001250"
     CDS             10967..11545
                     /locus_tag="KMT45_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBE7004721.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11549..12070
                     /locus_tag="KMT45_001251"
     CDS             11549..12070
                     /locus_tag="KMT45_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBE7004722.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(12149..12703)
                     /locus_tag="KMT45_001252"
     CDS             complement(12149..12703)
                     /locus_tag="KMT45_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBE7004723.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12956..13231
                     /gene="rpsP"
                     /locus_tag="KMT45_001253"
     CDS             12956..13231
                     /gene="rpsP"
                     /locus_tag="KMT45_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBE7004724.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13243..13494
                     /locus_tag="KMT45_001254"
     CDS             13243..13494
                     /locus_tag="KMT45_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBE7004725.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13618..14409
                     /locus_tag="KMT45_001255"
     CDS             13618..14409
                     /locus_tag="KMT45_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004726.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14579..15100
                     /gene="rimM"
                     /locus_tag="KMT45_001256"
     CDS             14579..15100
                     /gene="rimM"
                     /locus_tag="KMT45_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBE7004727.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15090..15851
                     /gene="trmD"
                     /locus_tag="KMT45_001257"
     CDS             15090..15851
                     /gene="trmD"
                     /locus_tag="KMT45_001257"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBE7004728.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15987..16334
                     /gene="rplS"
                     /locus_tag="KMT45_001258"
     CDS             15987..16334
                     /gene="rplS"
                     /locus_tag="KMT45_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBE7004729.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(16466..17602)
                     /locus_tag="KMT45_001259"
     CDS             complement(16466..17602)
                     /locus_tag="KMT45_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE7004730.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPNLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKMPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPKGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(17719..18411)
                     /locus_tag="KMT45_001260"
     CDS             complement(17719..18411)
                     /locus_tag="KMT45_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004731.1"
                     /translation="MKKVILGLVFGAFFLTGCSQSNVVDNTGKTNEKDSSVTLSESTT
                     QSSKNSQEVDGPLLKVGQWTKEKRDTSGKIELIGTASPQSDIPLGDIGIYISNIKLLK
                     YSNYADPSAAGYYDANNHKDSEGNFYGLQIAFKVKNNSEVDYGYNGLAHAILDNGQQI
                     DFSSDDLMHTMTTNSFFKKTESKEFYSLAYLDPSKVNDITKVTIKTGVLYNPSDYSTV
                     AESAESTFNINR"
     gene            complement(18549..19202)
                     /locus_tag="KMT45_001261"
     CDS             complement(18549..19202)
                     /locus_tag="KMT45_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004732.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     GQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(19252..19680)
                     /locus_tag="KMT45_001262"
     CDS             complement(19252..19680)
                     /locus_tag="KMT45_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBE7004733.1"
                     /translation="MEMDTINLVEELKRKYRSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIDELKNLYGLPESAYRFLI"
     gene            complement(19685..20059)
                     /locus_tag="KMT45_001263"
     CDS             complement(19685..20059)
                     /locus_tag="KMT45_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7004734.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            20344..20529
                     /locus_tag="KMT45_001264"
     CDS             20344..20529
                     /locus_tag="KMT45_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7004735.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            20542..20790
                     /locus_tag="KMT45_001265"
     CDS             20542..20790
                     /locus_tag="KMT45_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004736.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKITPKEFIEKHLVDL"
     gene            20821..21000
                     /locus_tag="KMT45_001266"
     CDS             20821..21000
                     /locus_tag="KMT45_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004737.1"
                     /translation="MGKFNRALVFSTPFIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(21043..21513)
                     /locus_tag="KMT45_001267"
     CDS             complement(21043..21513)
                     /locus_tag="KMT45_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004738.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            21600..22298
                     /locus_tag="KMT45_001268"
     CDS             21600..22298
                     /locus_tag="KMT45_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBE7004739.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22295..22468
                     /locus_tag="KMT45_001269"
     CDS             22295..22468
                     /locus_tag="KMT45_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004740.1"
                     /translation="MELKRKEVDHSEYTFAALCRIHHTEKHKIGVTNFKNKYQIKGIK
                     LNQETIKKLRIGG"
     gene            22471..22698
                     /locus_tag="KMT45_001270"
     CDS             22471..22698
                     /locus_tag="KMT45_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004741.1"
                     /translation="MINLNAVAFEQTWRTKYKKMSPRDKLFLEIMTFAFIGTQAEQSD
                     ISVEKIKTNRLVNGITETCYQYTIIVVDEEE"
     gene            22701..23528
                     /locus_tag="KMT45_001271"
     CDS             22701..23528
                     /locus_tag="KMT45_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replisome organizer"
                     /protein_id="HBE7004742.1"
                     /translation="MAERRMFAKTIIDSDAFLDMPLSTQALYFHLSMRADDDGFINNP
                     KKIQRMVGCGDDDLKLLMAKRFILVFESGVIVIKHWKIHNYIRNDRYKPTLYQDEKAL
                     LADKDNKAYTFAEELSKHDEKLGIPDDNQAVYQMDTQVRLGKDRLGKDSKEIKDLTPS
                     KKSKGKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESSGKTYKNYL
                     ATIRSWARKEKSEPKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            23540..23809
                     /locus_tag="KMT45_001272"
     CDS             23540..23809
                     /locus_tag="KMT45_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004743.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            23971..>24122
                     /locus_tag="KMT45_001273"
     CDS             23971..>24122
                     /locus_tag="KMT45_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004744.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIA"
BASE COUNT         7894 a         4216 c         4809 g         7203 t
ORIGIN      
        1 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
       61 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
      121 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
      181 ggcctgatcc aaggaagcgg atttgtggtt tctaggaatt ttccagcttt gattgttttc
      241 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
      301 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
      361 ttgattatcg gccctaatgg tttgagcgag agtggattga aggatttagc ttattcctcc
      421 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
      481 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
      541 aataactatt gatggtggat catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt
      601 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
      661 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
      721 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
      781 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
      841 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
      901 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
      961 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     1021 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     1081 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     1141 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     1201 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     1261 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     1321 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     1381 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     1441 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     1501 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     1561 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     1621 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     1681 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     1741 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     1801 aaaaaagagc tacgacatag aactgtcgta actttttttg gctcttcgtc aaatcgtgtt
     1861 ggtgaagaca aatttgaata aaacttagtc cgatatgcgg cgcgttctgt gcgccgcata
     1921 tttttttagt tttcaaagat cagaccttgt tgtaaataga tccgtgcccg aaagttataa
     1981 aagttgcgat aaccaaacgc aattcgttta atcagcttga tcttgttatt cagcccctca
     2041 gtgacgccgt ttgagtggga caccttaaag gcatttgaaa tacccgattg gtagcaatgg
     2101 aagatcgtga atttcttttt aaacgactga ggtaacgtag atgaaattgt tttaagggtt
     2161 tcttgaaagc cttccgtacc tcgtttaacg aaataatagc gtaataattg acaaaaatga
     2221 tacgcagttc gcaattcttc gttgtagctt aacagctcat caacgatttc tgtttgacta
     2281 attggtcgtt taaacagtcg atgataatgc cgtttgagtg gctcccatgt cgtggaatct
     2341 ttgagtaaga gtttccaata tcgcttgagc cgacggtact tcttctgatt ttcagagtgt
     2401 gaggttcgaa aacgattcat gatctggaca cgtaattgat tgaagctgcg attgatgtgt
     2461 tggacaatat gaaagcggtc agttacgatc tccgcatttg gaaagactgt tttaagcaac
     2521 tgacaatagc tggcattcat gtccatcact aggtacttga cctttaaacg ggcctttcta
     2581 ggaaagcgca ggaagtattg agttagtttg taaagtctgc gatcttcaag gatcgtaaat
     2641 agctgattgg ttgtcccatc aacacaaata aaactcatgg aaccagaaac agaattcatt
     2701 gatttgaact catccataca caacaccgtc ggaaggaagt tcatacgtgg ctggtaatgc
     2761 ttcgttgctt cacgcaagat ccgtgtcacc gtcccatccg agacgaagta cttcttagcg
     2821 attagtttac gtgattgatt ttcgtacagt tccatgagaa tcgcctgctt cagttccttt
     2881 gttagttgat gatgttcatc aactaagtcc gttttcgcag aaaaggtcct tccacaatca
     2941 tggcagagaa aacgggtacg ttttaattct aaaatagtta acttttcttt gactttcaac
     3001 atctgagtgc gtgtcgtata ggaaccgttg cggataatac gaaccgatcg acagtgttca
     3061 caataggaag aagagtcatc caattttcct ttaataaact gcgcttgaac gcctttatat
     3121 tctttttcaa tcagccattc ttcatcaaaa aataaatgtt tatctgttaa tccgagtaat
     3181 tttctagtat gattatccat tgagagactc ctttggtagg atgacgtttt ggtcgactaa
     3241 attttaccaa aagtgagtct ctttttgagt tgaaacgaag aaaaagtgtt agtagaaaat
     3301 cgccttcgat tttccaccaa cacaaaatat tatagaaccc tttttttttg ctatatgaca
     3361 ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt tccatatctg ttctatactg
     3421 gataggagaa gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc acatgaagaa
     3481 taaatggata aatcgaagta aaaaaaagaa agtaacacaa agatcatcag ataaagtcat
     3541 ccgtcttttt ccatcgtata aagaaggatt aacggcagat caagtcaagg aacgaataga
     3601 aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa acaaatcagc aaatcgtatt
     3661 ggaaaacata tttacgtatt tcaatttgat ctttcttatc ttagccattt tgctttgttt
     3721 agtagaatcc tataagaact tgactttttt accagttatc atagccaata caggaatcgc
     3781 catttttcag gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt taaatacagc
     3841 aaaagtcaat gtcatacgag acgggataga acaaacaata gatatagaag accttgtaat
     3901 agatgacatt gttattttag agactggata tcaaattcca gcagatgcag aggtcgtgga
     3961 agggaaactg caagttaatg aggctttatt gacaggcgaa gcagatgaaa taaccaaaga
     4021 aataggtgat ccattaatgt ccggcagttt cattgtttcc ggaaaagctt acgcccgatt
     4081 agacaaagtt ggagcagatt cgtatatttc ccagctgaca gtcaaagcca agacaatggg
     4141 agaaggagaa cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat ggattgggat
     4201 catcattatt cctatcggta tcctgctttt ttctcaaagt tactttttta atggaaacac
     4261 gatcaaagaa agtgtcgttg caatggaagc agcattgatc ggtatgatac ctgaaggact
     4321 gtacttgctg acgacgattg cattagcatt aagtgcgact aaactagcaa agcaaagagt
     4381 tcttttacac aatatgaaaa gcattgagac attggcaagg gtaaatgtcc tttgtgtaga
     4441 taaaacagga acgatcacag aaaataaaat gtctgttcaa aaaatgatcg tctctaaaaa
     4501 acaagaaata atcagtcagt cagatcaatt ggaagcatgc attgctgact atgcaaaagc
     4561 gatggcagga gacaatgcaa caatggaagc tgttaaagca tattttacaa aaaccactga
     4621 tcaatcctat caccatgtta ttcctttttc ttctgtccaa aaattcagca gtgtagcgtt
     4681 ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc aattgccgga
     4741 atatgcggaa gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg tctttggtgt
     4801 gtacgatggg attcttgaag aaccagtatt gacagaagca gtcacaccat taggctatat
     4861 tctgattgct aatcctatca gaaaagaagc aagagccaca tttgattact tcaaaaaaca
     4921 gcaagtagcc atcaaagtga tttctggaga taatccgctc accgtatcca atgtagctgt
     4981 ccaagcaggg atttcacatg ctgatcagta cgttgatgca tctaaattat cagaagaaga
     5041 atatgaagca gctgtcgaaa aatatacagt ttttggacgc gtcaagccag aacaaaagaa
     5101 aatcttcgtt caattgataa agcgtaacaa tacagtagct atgacaggtg atggcgtgaa
     5161 tgatattcta gccatgaaag aagcagactg cagtatagcg atggcttccg gaaatgaagc
     5221 cgccatgcag gcctcacaag tggtcttact tgattctgac ttttcacgca tgccagaagt
     5281 tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga tcagccagct tgtttttagt
     5341 gaagaatatt ttctcctttt tgctctcact gttttcagtg atctttgctc tgacctatcc
     5401 tttagaacca tcacaaatca cattgattag tttgtttact atcggacttc catctttctt
     5461 gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga atgtgatgga
     5521 aaaagctgtg ccaggtggac taacagatat gatcgtagtc ggtgcgttgg tgatctgcgg
     5581 ggtgactttg aatctaaata aaacagatgt ttctacagca tctaccatgc ttttgattgc
     5641 agtagggttc ctcgttctct ataaaatctg ttcaccgctg aataaattca gaagtcagat
     5701 cattcttttc tgtgctagcg gtaacttttt ttcggtcatc tttttgcata agctgttttc
     5761 aatcaccggt atctcagctg tatcactttt gctgttgagc attctatttt tctcggcaga
     5821 ttcgattttt aggcatttga ccacattggt agaatatttg tttgaacgta aaaatgaaaa
     5881 agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta tcgatattta aacgagaaaa
     5941 ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta tctttcgaag
     6001 caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac attttcggaa
     6061 tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc gtaccattaa
     6121 ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct gaatgtcggc
     6181 ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc tcaatgtcga
     6241 tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga tagcggataa
     6301 taatctcgct gtcgcttttt gaatattgga cagcattttc aatcagattt ttgataattg
     6361 gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat cgaatcgtca
     6421 accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa taaggttctg
     6481 tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg atgatttccc
     6541 gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa gaagtcagag
     6601 tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc agtgaagtca
     6661 caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc ttttctaaac
     6721 gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct tctgaattag
     6781 taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg atctcctgat
     6841 ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt ggctcttgaa
     6901 tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt agctgcatct
     6961 ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg ccaatcatca
     7021 gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga tacatcttac
     7081 tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga tcgggtgttt
     7141 gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg ggcagcacgg
     7201 ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca agaacaagga
     7261 agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc ggtaaaaaaa
     7321 cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt ttgactggta
     7381 atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg ctacctaggt
     7441 tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca ttcaatgttt
     7501 cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc cgttcgttcg
     7561 actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg ttttcgacat
     7621 ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc tgttccacga
     7681 tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa ctaactaaaa
     7741 gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg gttcctgaaa
     7801 cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat ctcgctcgat
     7861 tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg cgaagtcaaa
     7921 attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc gtttcatgaa
     7981 ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt ctacggtcac
     8041 ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag gctcagcttc
     8101 atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac gggctaatac
     8161 ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta ggccaatgat
     8221 ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat ttttttcttg
     8281 acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat ctaaaatgat
     8341 gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg tggcactggt
     8401 taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag aaggttcatc
     8461 atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat tctgtttata
     8521 ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg tataatagga
     8581 aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg catgtttagg
     8641 cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga tggaactagt
     8701 tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac caattcctag
     8761 agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt
     8821 atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga tcaaagccta
     8881 tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa gcccttcatg
     8941 cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg gtgccggagt
     9001 ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag aatggctgaa
     9061 aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat
     9121 tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat tatgcagatg
     9181 atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca gtatatgaca
     9241 atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat cttttttcta
     9301 attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca gataagtacc
     9361 cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa gaataaataa
     9421 aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc aggcgatggg
     9481 aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga tgcgggaaat
     9541 ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg
     9601 cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg cacaacaaat
     9661 cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag cagagttaaa
     9721 taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg caggtaaaac
     9781 gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc gtccgctcct
     9841 aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca
     9901 gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga tcgtccgtca
     9961 agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata cggcaggacg
    10021 tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat tgacgcaacc
    10081 aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca acgtagctga
    10141 tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg atggggatac
    10201 tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca aattcatcgg
    10261 ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg ctagccgtat
    10321 ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact acgacgagaa
    10381 aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca atgactttat
    10441 tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca aaatgatccc
    10501 aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg
    10561 caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa
    10621 cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg
    10681 aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca aaggggatat
    10741 gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat
    10801 ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga aataagatga
    10861 aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct atcagcaatt
    10921 tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga gatgtccgtg
    10981 gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg ttcctgaata
    11041 tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg gattgagctg
    11101 gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat tcgatattaa
    11161 aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca aaggaattat
    11221 tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca
    11281 agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa aaatcgttga
    11341 ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag atgaattagc
    11401 aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct atgctttttt
    11461 agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag aagaggtcgt
    11521 acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg gtgctataag
    11581 tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg acactccaac
    11641 aggtaagcaa accacttatg aagcttttta tgaaacaatc gatacgacca tcatgggcag
    11701 aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa
    11761 gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg tcgtaaacga
    11821 agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata tctgggttgt
    11881 gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg aatggtatat
    11941 ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag gaacatatga
    12001 agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat tgcattatgt
    12061 acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt cctgtacata
    12121 gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa aaatctagat
    12181 acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta ccattttcat
    12241 caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt ggcacattca
    12301 aaaagacaag tgattgtcgt agatggtggt ttgcgccaaa gccgccgatc ccacctggtg
    12361 atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa ggtcttgagc
    12421 ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca ggagtaacga
    12481 aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca gcaggagcct
    12541 gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt acaaatacac
    12601 tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg ttgtaagaat
    12661 cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt ttcctccttc
    12721 aaactatcta taacaatctt acaaaaaata agtaactgga gcaaataaaa acacttactg
    12781 acaaaagcta catagaagat agattactta tttttaacaa aaaagaagtc taaaatcttc
    12841 tatttcctaa aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa
    12901 aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg aggtggaaat aaataatggc
    12961 agttaaaatc cgtttaaaac gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt
    13021 agctgattca cgttctcctc gtgatggacg tttcatcgaa actgtaggta cttacaatcc
    13081 tttgaaagac cctgcagaag tagttttaaa agaagactta gttcttgact ggttatcaaa
    13141 aggtgcacaa ccttctgaca ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa
    13201 acaccacgaa gctaaattct caaagaaata aggtgaccgg atatgaaaga tttaagcgac
    13261 ttagtcttaa caatcgttcg tccgctagtc acttatcctg atcaagtcaa gttagacgtc
    13321 gtagagtcga aagactttta cgagtacaat ctaacagtgg ctcctgaaga tattggccgt
    13381 atcattggaa aacaagggcg tgttgctaaa gcaatccgca caatcgttta cggtgttcgg
    13441 atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac
    13501 gtgctgtact cgaattttcg agtgcagctt ttttgttttt ttattaattg aagaaatgat
    13561 tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga acagccaatg
    13621 agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag ttcggttact
    13681 caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac cagtccagaa
    13741 aaaagtagta ccaaaacggc taccaccact gaacaaacaa tggctacctc aaataatgag
    13801 aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat gcctctagat
    13861 gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca aggttattct
    13921 attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt gaatcaagaa
    13981 acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga aacaatccaa
    14041 gttttgcagc cctttgagat cgacacaaat ggtcagcaag tggacttagg ttctcgaatc
    14101 acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga aggaaactgg
    14161 cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt agctaaagaa
    14221 atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg taaaattact
    14281 gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga accgaaaaat
    14341 gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt ttctatgaag
    14401 aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc acaggaagta
    14461 atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa aagaaaacta
    14521 gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg gatataagtt
    14581 gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag gcgaagtccg
    14641 tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg tgttaacact
    14701 attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca aacataaaaa
    14761 ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa aatatcggga
    14821 tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg aattctatta
    14881 ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta aagtaaaaga
    14941 aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa aagctgatat
    15001 attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg gagtggtccg
    15061 tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact ttatttccaa
    15121 gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat aaaggattat
    15181 tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag acagtagatg
    15241 attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc tatgataaca
    15301 tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt cttgacccag
    15361 ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa cacttagtat
    15421 tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg acagatgaag
    15481 tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc atgatcgatg
    15541 caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa acggattctt
    15601 attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat aaagggatga
    15661 aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg caattaaaag
    15721 aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt gaatggacac
    15781 ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa acagcagatg
    15841 aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg ttagtatttt
    15901 atggtgagtg caaaagcact taactattac gatattccgc tatgagaaaa gctcataaga
    15961 atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta acaaaagaac
    16021 aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt cacgcaaaag
    16081 ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc aaacgccgcg
    16141 gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg ggcgttgaac
    16201 gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc tacggtaaag
    16261 tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct cgtatcaaag
    16321 aaatccgtcg ttaatcattg ttatcagact gagaaagaac ccttggtatt caagggttct
    16381 ttttttatta tctgtttttt tatattaacg atttctattt ggtacatttt tgattaataa
    16441 aaaggggcaa aaaaggggca acaagttaca gtttttctat atcttctttc aatttttctt
    16501 ttactccttt tgtcacatgc aaataaatct tttcagtgat atcactattt tcgtgcccaa
    16561 cgcgatcctg aatagcatac agaggagttc ctagttccgc taattttgaa atatgggtat
    16621 gtctaaaaat atgtgaacta agttttttat caatcttcat gtcagcttta tgatttctca
    16681 aataggtgtt aattgctgtt agttggaaag gagttccttt cgttgtttga aatagaaatt
    16741 gacccttcgg atttaattct agaagctcat tgtaaatagc tatcgctttt tttggtaaat
    16801 caatttctcg cattccagca gcagtttttg tagaatcaga ttttttcata tcagctattg
    16861 aacgttctct atacatcatc gtcccattta taactactga tgcattatta ttggtaatgt
    16921 gtacatcgtc tttacttaat gcgattgctt ctccaggtct cattccagtc aaatatagcc
    16981 actggaaaag gagagaatat cttttattgt gtgaagttgt gaaatctact agtcttttat
    17041 attcatcatc ttctaaaaac ttatctttaa tttttatcgt ttttgactct cgtttataat
    17101 caataactac ttcgttgata ggattttttt ctacatagcc ttttttcata gcgtatgaaa
    17161 aaagaagatt caatttggat ttaattacac tgacgtactt atttgacaag tcatctttat
    17221 atatcatatc ttcaaaaata ttatttaaat ctactgtatt aataccagaa acgatgtaag
    17281 tctcaggcat cttcttcttt atagtgttta agatattatt cgtaggataa tatgtagact
    17341 ccttaacttg ccttttgtaa ataactaacc attcttcgac aagctcatga aatgttagat
    17401 taggcttctg gagtgttttt tcatttagtt ttatatcaat ttttttattt aactccagta
    17461 atgccacttt ctgagtttct cgagacttat ttttataagt aatgctcact ttttttcttt
    17521 ttctagtttt tggatcaata tatcgttcat tatatttata tacttttttt ccgtttttat
    17581 cagttttggt ttcaatccac attgttttca tctcctattt gttgctataa taggaatgga
    17641 taagtaagcc gattatagca ggtttatttt tcatcacgtc cacaaacttt ggcgagggag
    17701 ggggcgtgtt tttgtatttt aacgatttat attgaatgtt gattcagcac tttcagcaac
    17761 tgtactataa tcagacggat tatataacac tccggttttt atcgttactt ttgtaatatc
    17821 gttaacttta gatggatcta agtaagctaa actataaaat tctttacttt cagttttttt
    17881 gaaaaacgag tttgttgtca ttgtatgcat aagatcgtct gaactgaaat caatttgttg
    17941 gccgttatca agtatggcgt gtgcaagccc attataacca taatctactt cagaattgtt
    18001 tttaactttg aatgcaattt gtaaaccata aaaatttcct tcactatctt tgtgattatt
    18061 tgcatcgtag taaccagcag cgctagggtc agcataattg gaatacttga gtagtttgat
    18121 attagatata taaatgccga tatcaccaag aggaatatct gattgtgggc ttgccgttcc
    18181 aattaattca attttgccac ttgtatctct tttttctttg gtccattgac caactttgag
    18241 gagggggcca tcaacctcct gtgaattttt agaagactgt gttgtacttt ctgataacgt
    18301 aactgaagag tctttttcgt ttgttttgcc agtattatct acaacattag attggctaca
    18361 tccggtaaga aaaaaagcac caaatactaa ccctaaaatc acttttttca ttctttattc
    18421 tccgtttcta tgatattatt tttatgtagg atcttagaaa cgagatctta gtccgtgttg
    18481 cagcacgggc ttttttcttt ataacttttt tagagattat aggcaaaata gtaggacaca
    18541 aaaatatatt attgtattcc gtatttagaa aatcctaatt gaacttcacc agaagttttt
    18601 tgctgtgttg tacgcaatgc ttcttcttca gacattccat tctgtacttt ccatgcaaca
    18661 ggcgacatcc cgtatttgtt aacaaaatca gtaagtgata aagtgtcagc gtcttgctga
    18721 ccgcttgttt gttggtcttc tggattttgt tgagatgctg cttgttgttc tttctgatct
    18781 tgactgataa tattgccagc atcatctgta gtcaatccat tttcataaag ggccacgccg
    18841 aaagcttccc actctttgtt ggaccaattt gcacgatcag ctggagttga ctgtaaagtg
    18901 cgttgtttca tctgttcata tgtttcttct tgaggtgcgg tttggattgt atcctgactg
    18961 gagcttataa ctgttgggct aggttccgcc gtagcttggt tagagcttgt aactgttgaa
    19021 ctagaatctg tcttagatgt agatttgcta gtagaggaac tggtttcagt tgtttctttt
    19081 gttttactta cttttgtttc ttggttagaa gtggcatctg ttgattcagc ttttttatta
    19141 tttgaacaag ctgaaagtag cagagcagta cttaacaaca acataacgct aacttttttc
    19201 attttataat tcctctttct cgttgtaata tgtgtgctaa cacgggcttt tttatataag
    19261 aaaacgataa gcgctttctg gaagtccgta aagattcttt aattcatcga tttttttggg
    19321 atattgatca ttatcttctt tataaagaga aacaatgaga ttagcagcaa agcaattagc
    19381 ttcgctttca gatttgcttc tagatgttct tgttgataca tagtaactgg ataagccgcg
    19441 atgaaaaata gcgtgaccta attcgtgagc gcaaatgtag aatctttcct cagagtctcg
    19501 cagttcatca tttaagaaga ttattgcacg acctctaatt tcttgaaact gtcctttggg
    19561 attttcgata aaaggaacgt attgaatttt aatgcccatc ttttcacaaa tataaaaagg
    19621 attagcagac cggtatttcc gcttcaactc ctcgactaaa ttaatcgtat ccatctccat
    19681 aagctcacat ctttttgcct ttttctttgt cttctttcac aatatcccag aaggttccaa
    19741 taaggatatc ttttacgcgt tggacctgtt caggcgtcag tgtttctccg ccataagaca
    19801 tattgacatt tgaatctagt aatttatcaa gttcaactac ttcatctttt gttgcccagt
    19861 caggaacttg gtttctaccg agtaaataat cagtagttac attaaaataa tctgctacaa
    19921 gttgtagtct tttagtacta ggagttctat ttttccattg ataaatcgtg tttttaggta
    19981 tatttaattc ttcttccaat tgagttatac ttatgcctct tttatgagct aattccttta
    20041 ttctatctag taaattcatt ttcgcacctc acaagctacg aaaacactaa taaaaaaatt
    20101 agttaaaggt gttgacaact aataaaattg ttagtatact gttttcgtaa gctaaaatat
    20161 tagctaaaaa gacttgataa ctaataaaca cttcacggtc ggcaaactct gaaatgtaaa
    20221 ttactaggca tttctgtgtc ttatttagct atatctatat actaataaaa atattagtta
    20281 ttgtcaacga atattagcta attttttagc ttacagacta ttttttagaa aggagctatt
    20341 tttatgtctg agaatttaga cttaaaaatt cgagcggaga tgagaaaaag aagaatgact
    20401 ttcaaagaac tagctgcgct tgttggtatt tcaggagctt atttatcaga tattctaaac
    20461 ggcaatcgtg atggaaagaa agcacaacag catatcgaaa cagtgaaaaa aatattggac
    20521 atccgatagg gggggtaggc gatgacaaaa ctaaaaaaac aagattttgt aaaaaaatac
    20581 aattattctc catctactta tcaacgtcga atgtcggaac taaaaaatac agcaattttc
    20641 tcagcggcgt atgaacgggt cacaggacaa gaagtttgga tcaatacaga attatacgat
    20701 aaatttttgt ctttcaaatc ctataacagg ttacgcacaa gaaagataac acctaaagaa
    20761 tttatcgaga agcatttagt tgatttataa aaaacagaac attttgagag gtgaaggtta
    20821 atgggcaaat tcaacagagc actagtattc agcacaccat tcatcatcta cgctttaggc
    20881 ctctggggaa gcaggcaagc gttgatagga acgattgttt acatggtttg gatttttatg
    20941 gggcttgatg aagctgaagc tgagtacaga gcgaaaaagc caaccgaggg ggctgactaa
    21001 aaatgaatag aaaagagaaa ctagaatgta tattactatt actcagttta attctttcac
    21061 taatttctct attgggaagt ttgtattttt gatatcaaca attttgtgga tttgggttct
    21121 atttagagta aatatcattt ttgtttggtg attaaggagt ttattaaaat taattttccc
    21181 tacttcaaat gcaaggtaac aagatatagc acttttagga ggaatattta gcggtacagc
    21241 ttcagagtat attctattat catcgactga tcctccttta gcaattaatt tcttaaatgg
    21301 agtagctgac catctgcggt ttaagttagc ggatgaaagt tctaaattaa ctaacatagc
    21361 tggttctgtt gaatagttag aaatgattac ttttgtataa atcatatctg cagcgaagta
    21421 agaagcatta agttcaactt ctagttgagg gcgcattcct tttattttta tccctgttaa
    21481 aaacgtactc aatataaaac ttattattga catccattga aaaattgtta agtgaaaaaa
    21541 attcaaaata aacaccacca gtttttaact aaattatacc aaaaaggaga gaagaaataa
    21601 tgcaagaatt agtaattttg aaaaataaag aagctgtgac tacgagctta caagtggcag
    21661 aaagcttcga gaaaaaacat caacatgttt taagagatat tgatgcacta aaaaaagatg
    21721 tgtccaattt tggacagatg tttgtagaag gtaatgaacc agattcatat ggcagaaatc
    21781 gacgagtttt cttcattagt agagatggat ttttcttgct ggctatgggg tttacaggaa
    21841 agaaagctat ctacttcaaa caaaaataca ttgaagcatt caacgaaatg gaagatgtta
    21901 ttcgcaagaa tactgttcct caaacaattg aagatatgat gatctatcaa ttagaagaaa
    21961 tgaaagatgt taaaaaagat gtttccatgc ttaaagatac tatgcgaatt agcggacaac
    22021 aagagtttga aattaagcaa aaaggaaata tgaaagttat gggagttcta gggggaaaag
    22081 aaagccgagc ttatgaagaa atcagcaaaa aagtattctc aaaattttgg tctgaattta
    22141 aacgtacctt ttcaatccca agatatggcg agttacctcg taagagattc gatgatgctg
    22201 tttcatttat tgaaatgtgg ttgccagaaa ctgcgatccg tatggaaatt gatcaactga
    22261 acagacaaca gagacttttt ggtgatgaca atgaatagag ctgaagcgca aagaagttga
    22321 tcattcagag tacacatttg cagcactctg tagaattcat cacacggaga aacacaaaat
    22381 aggtgtgacc aatttcaaaa ataagtatca aatcaaaggg attaagttaa accaggagac
    22441 aatcaagaaa ttaagaatcg ggggctaatg atgattaatt taaacgcagt agcgtttgaa
    22501 caaacatggc gcactaagta taaaaaaatg agtccaagag acaaattgtt cttagaaatc
    22561 atgacatttg ctttcattgg tacacaagct gaacaaagcg atattagtgt tgaaaaaatt
    22621 aagacgaata gattagttaa tggaattaca gagacttgtt accagtacac gattatcgtc
    22681 gtggatgagg aggaataatt ttggcagaaa gaagaatgtt cgcaaagaca attattgatt
    22741 ctgatgcatt cctagatatg cctttatcga cacaggcatt atatttccat ctatcaatgc
    22801 gcgcagatga tgatggattt attaataatc ccaaaaaaat acaacgtatg gttggttgtg
    22861 gtgatgatga tttaaaactg cttatggcaa aaagattcat cttagttttc gagagcggtg
    22921 tgatcgtcat taagcattgg aaaatacaca actatattcg taatgatcgt tacaaaccca
    22981 cgttatatca ggatgaaaaa gctttactag cagacaaaga taacaaagct tatacatttg
    23041 cagaagagct ttctaaacat gatgaaaaac ttggtatacc agatgacaac caagcggtat
    23101 accaaatgga tacacaggtt aggttaggta aggataggtt aggtaaggat agtaaagaga
    23161 taaaagattt aacgccttcg aaaaaatcga agggtaagcc catccgtcat aaatacggag
    23221 agtataaaaa tgttcttttg tcagatgagc agatggagaa actcaaaaca gaattcccta
    23281 atgattacca agagcgaatc gaacgactgt cagagtattg tgaatcatct ggtaagactt
    23341 ataaaaacta tttggcaact attcgaagtt gggcaaggaa agaaaaaagt gaacctaaga
    23401 acgcaagcag tggatacaag cgcacaggaa gacgagagaa gcttcctgaa tgggcaatcg
    23461 accaagaagc ctatctcaag aaaaaagcgc tagaacgagc taatagacaa tcaaaagcac
    23521 cattttaaga ggtggaaaat tgaaaatcga ttatctagaa ctaattaatg aaatagcaaa
    23581 gtataaaact ggtgaggaaa tagaaatcct gagagacgta tatgatcaac tcgaagaagc
    23641 tggaatcgaa ggaattaata atgatcgttc gagttggagt aaactcagat actatttcgc
    23701 actctatatc gatacaacac aattaagaaa tttagcttat acaaaattac tatttgttga
    23761 ttgcgttaaa ggattgcaaa aacatcttag tgaacttgag caggtgtaat cagatggatc
    23821 taaaaacatt tacagcacag atcgaactaa tgcatcaaga agctttaaga caaagcgcct
    23881 cgtacgaaga caagtggctc aacacgttcc atggtggacg tgagagcgca cttgatcaag
    23941 tactcaaatt attgaaaggg gaacgtcggg atggataaga aagcggcaat gaaacgaatc
    24001 atcgaactga cacattctga gaattggcaa gaagacaaag aaatagttac agaagtccaa
    24061 aagctcggta aatcaatgtg gactgaaaag cccaaacgga gaacgccgag aaagattgca
    24121 at
//
LOCUS       DAEGIQ010000035        23981 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000035 DAEGIQ010000000
VERSION     DAEGIQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.035"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            30..3320
                     /locus_tag="KMT45_001274"
     CDS             30..3320
                     /locus_tag="KMT45_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBE7004745.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGPDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKDHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERLTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNQATGEVLENAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     ASYETKEILHTVDYRKPNYSTVYVTPEGSDTGNGSRQNPLSLTQALRYAYAGQTIVLA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWVLR
                     GFDVTNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVVIK
                     NSVAYRNGWVPGVEGEGNGNGFKMGDSSLSGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDFHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTHSQEGQQPVTRYANGSIHVHGLMQIKPEN
                     RQTEKGRVDGLPLSVGATFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVV
                     YVMKQRKK"
     gene            complement(3738..5645)
                     /locus_tag="KMT45_001275"
     CDS             complement(3738..5645)
                     /locus_tag="KMT45_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBE7004746.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5775..6650
                     /locus_tag="KMT45_001276"
     CDS             5775..6650
                     /locus_tag="KMT45_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBE7004747.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6730..7704
                     /locus_tag="KMT45_001277"
     CDS             6730..7704
                     /locus_tag="KMT45_001277"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBE7004748.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7883..9028
                     /locus_tag="KMT45_001278"
     CDS             7883..9028
                     /locus_tag="KMT45_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBE7004749.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSSEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9165..9509
                     /locus_tag="KMT45_001279"
     CDS             9165..9509
                     /locus_tag="KMT45_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBE7004750.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9780..11066
                     /gene="efmA"
                     /locus_tag="KMT45_001280"
     CDS             9780..11066
                     /gene="efmA"
                     /locus_tag="KMT45_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBE7004751.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11204..11803)
                     /locus_tag="KMT45_001281"
     CDS             complement(11204..11803)
                     /locus_tag="KMT45_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBE7004752.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELITQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12207..13328
                     /gene="mnmA"
                     /locus_tag="KMT45_001282"
     CDS             12207..13328
                     /gene="mnmA"
                     /locus_tag="KMT45_001282"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBE7004753.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGDMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13574..14995
                     /gene="arcD"
                     /locus_tag="KMT45_001283"
     CDS             13574..14995
                     /gene="arcD"
                     /locus_tag="KMT45_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBE7004754.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15009..16127
                     /locus_tag="KMT45_001284"
     CDS             15009..16127
                     /locus_tag="KMT45_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBE7004755.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVNPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16187..17479)
                     /locus_tag="KMT45_001285"
     CDS             complement(16187..17479)
                     /locus_tag="KMT45_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBE7004756.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMSFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            17611..18189
                     /locus_tag="KMT45_001286"
     CDS             17611..18189
                     /locus_tag="KMT45_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE7004757.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINDFIATLP"
     gene            18318..18695
                     /locus_tag="KMT45_001287"
     CDS             18318..18695
                     /locus_tag="KMT45_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBE7004758.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18628..20700
                     /locus_tag="KMT45_001288"
     CDS             18628..20700
                     /locus_tag="KMT45_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBE7004759.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLNLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERIPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            21077..22120
                     /locus_tag="KMT45_001289"
     CDS             21077..22120
                     /locus_tag="KMT45_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBE7004760.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLFYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(22182..22646)
                     /locus_tag="KMT45_001290"
     CDS             complement(22182..22646)
                     /locus_tag="KMT45_001290"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBE7004761.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            22892..23839
                     /locus_tag="KMT45_001291"
     CDS             22892..23839
                     /locus_tag="KMT45_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBE7004762.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
BASE COUNT         7639 a         4373 c         5084 g         6885 t
ORIGIN      
        1 ctttctaatt tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc
       61 taatgatgaa tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg
      121 ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa
      181 caatggagga agtaccaaga actagtgaag atatggatac ggaagaagga aatgatccta
      241 tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt
      301 ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag
      361 aagacggcgt gcggctctct tccacgaata acggcggaaa attccagtca agcggaccag
      421 atggaatgac ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga
      481 cgatcgaaat agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa
      541 gagacgcagt accaaaagat catttgtttg ggcaggcatt ttattcaaat tcatttgcca
      601 tactaggcag tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga
      661 gcggcagcaa atatacgatg cgacttggat tagggacacg ggcaatcaca ggaatatcat
      721 ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag
      781 agacttcagc agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc
      841 atgggaatct gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa
      901 cgaatactgg tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg
      961 acatcatgta tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat
     1021 ttgctgcagc aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcggatc
     1081 ctgcgacaga tcctcctggg caagaaagac ttactcgctt gattgatccg atttacactg
     1141 tgacctctag taatcattca ggaaaccaag accatactat gtcctttaga gcaaatgctg
     1201 acggattatt aacaatcaaa aatcaagcaa caggagaggt acttgagaac gcaaaagact
     1261 tggcaatcac tgcaaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg
     1321 aatttacttt cagttttaca ccagataaaa actatccgcc agaagaatac gtagagatgg
     1381 ctagttatga aacaaaagag attctgcata cagttgacta ccgtaaacct aactattcga
     1441 cagtttatgt aacaccggaa gggtcagata caggaaacgg aagcagacaa aatccgttat
     1501 ctctgacgca agcattgaga tacgcatatg ctgggcagac aatcgtcctg gctcctggaa
     1561 cttattcctt tgaaagagga ttgacgattc caaaaggagt taacgggaca agagaaaacc
     1621 cgattcaatt gattgcagag aaaaatccag aaaacaagcg acctgtactt gattttaaag
     1681 gaactggaaa tgggatgaca ctgtttagtc attactgggt tttacgtggg tttgacgtta
     1741 caaatagtgc cgctatgcaa aaaggattgc agatttctgg aaaccataat ttgattgaag
     1801 agatccatgc ttatcggaat ggaaatacag gaatccaaat cagtggtagc tcaaatgatc
     1861 ctttcgaagc atggccagcg cataacttag tcaagaactg tacatctttt gaaaatgccg
     1921 atccaggatt tgaagatgcc gatggatttg cggccaaact gacaatagga gaagggaatg
     1981 tatttgacgg ttgtatcgct tatcataatg cagatgatgg ttgggatctt tttgctaaaa
     2041 atgaaacagg ttcaatcggg aaagtagtca tcaaaaactc agttgcttat cgaaacggtt
     2101 gggtaccggg agttgaagga gaagggaacg gtaatggatt caaaatgggt gattcctctt
     2161 tatcgggccc tcatgaattg atcaatagtt tgtctttcga aaatcttgca aaaggaatcg
     2221 attccaatag cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat
     2281 ataatgtggc tctttatacc tcttcggcag gaaacacaga cttccatgct tctggtatct
     2341 tatctttccg aacagaaaat cttgagatga gagagcagat ccgcccattg aatcaaaacg
     2401 aagatcaaat ctatcactcg ttgaactact attggaatga acgagagaaa caatcagaaa
     2461 atactcttgg acataccgta tcaaaagatt ggatcgaatc tctaaataca cattcgcaag
     2521 aagggcagca gcctgttact cgctatgcga atggaagcat ccatgtccat ggattgatgc
     2581 aaatcaaacc ggagaatcgt caaacagaaa agggaagagt agatggtctt ccactatctg
     2641 taggtgctac ttttgacgga gaaacctcac cgtcttcgga gtatccagaa tatgcaatcg
     2701 tacaagaacc aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag
     2761 caaatggtgt ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt
     2821 ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc
     2881 cagaaaagat ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta
     2941 ttttttcagc accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat
     3001 ttgatgggga aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg
     3061 tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa
     3121 atgaatcacc aaatggaaaa ccaaatggaa aaccaaatgg aaaaccttct catccggata
     3181 ggacagcaaa agaaagtgaa aaaaatggca ctcaaggact cccaaaaacg ggagaattta
     3241 ataacccgtt gctagcatta gctggaggaa ttttgttaat cggtgtagtt gtttatgtca
     3301 tgaaacagcg aaaaaaataa gtggaatttc tccttttatg atgtaaaaca attgactgag
     3361 gaaagtaaaa atagaaggaa atgagaccaa tagatgtctc acttccttct atctttttat
     3421 agatgaggtt ctccttgctg cttttgattg gctgttctta ttacttgatg caggacagct
     3481 ttttcacaat ctctccacta aacggtgttc aatcagctga ccctcggtat tagctcaaca
     3541 aacggcaaaa tatgaagaac tatttttccg tttttttctc taatactcag gtcaggacgc
     3601 tgatttcaca acctctcgat aaacggtgtt caaaagccag tttctcagta gtaagtcaaa
     3661 tttatgcaaa aatgtggaaa gctattttca caaaattttt cttactactt gaaactaagc
     3721 ggctttttca caacctctta ccagattcgc actctttttt ctggcgctag atacatagcg
     3781 tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg ttgcacgttt
     3841 gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg gtattctttt
     3901 tttgcttttg tacgccaaat cgttgcccaa ttgacaaaaa attcctcgat agaaagatct
     3961 gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc aatattttcc
     4021 gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc gaattcttcg
     4081 atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca ccagttgttc
     4141 aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga aatctcatga
     4201 gcgataacag ctccgattcc accataatta gcagagcttg attgttctag actgtaaaat
     4261 ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg ataataagca
     4321 ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt ccatttgctg
     4381 aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa agtaccaccc
     4441 tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc ggggtagcct
     4501 acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc atccccaagc
     4561 cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac catttgttct
     4621 acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc aactacttga
     4681 tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc ttcgtctgtt
     4741 ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga tagataacca
     4801 ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa atgagcttct
     4861 gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac tttatctgga
     4921 gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt gtattgaata
     4981 aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc ttctgcactt
     5041 tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat tgcttctgcc
     5101 tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa gaaaacgcct
     5161 attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg aaggaataaa
     5221 gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc atctacatcc
     5281 agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctc attcaattcg
     5341 tcgaaagact gcaatgattc aactttttcg attataggtt gaagtggttg agcatgcaac
     5401 tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg tttcattttt
     5461 tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat caaaagttgg
     5521 tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc agggattttt
     5581 gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa tcgttctttg
     5641 ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa aatgaaggaa
     5701 ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt gtttttttca
     5761 atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta gtggataaac
     5821 ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag cagctgcagt
     5881 caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt tatgtgatta
     5941 tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat caagagggtc
     6001 tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct agatggatca
     6061 acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag gaactcgcaa
     6121 attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg gctaccccct
     6181 caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat caagactatc
     6241 gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat tcgttggttt
     6301 tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt ccttttcagg
     6361 ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt gcacgagcaa
     6421 ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa cctgtgatga
     6481 ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca gcgtacgctt
     6541 attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg ggcgattatc
     6601 ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa gaatttgctg
     6661 aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag aagttttggg
     6721 gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag tttgaatggc
     6781 aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg taaaagcgtc
     6841 gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat tcgaggggat
     6901 catgtgtata tcgttcaatc aacgaatgca cctgtcaatg actactatat ggaactactg
     6961 atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt attgccttac
     7021 tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac tgcgaaattg
     7081 atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga tctgcacacg
     7141 gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat gccgttgttt
     7201 gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt ttcgccaaaa
     7261 aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac attagccatc
     7321 gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga tgttcaagga
     7381 aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc acgagcagca
     7441 aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca tggattattg
     7501 tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg tatcactgat
     7561 tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac atgttcagaa
     7621 ttaatgggag aagcggtcaa acgcatccat gaaaatacac cgatgagtcc gttattccgt
     7681 ctggaagaaa aagaattcga ataaacaaaa aaagagggaa agaagttgtg acacaagtga
     7741 gcagcttcaa ggagctgcga caagactttt gtcacagctc cttttttcta tgcccatgaa
     7801 acgtcctcta gacaaaaatg agctcttctt tggtttgttc gctattttat gctagaatgt
     7861 gcctagcaat aatttggaag ggatgaatga agtgaaaact atttatctgg atcatgcggc
     7921 aactaccccc atgcatccac aagtaattga agcgatgacg gcaatcatgc aagacacgtt
     7981 tggaaatcct tcaagcatac atggatttgg tcgccacgcg catgaaaaat tggaaacagc
     8041 aagacaaatc attgcagaca gtctgcaagc aaaacctcac gagatcatct ttaatagtgg
     8101 tggaacagaa ggggataata cagctatctt agaaacagcg ttctcgcgtc aaaaagaagg
     8161 cagacatcta atcactacag ctatcgaaca tcctgctgtt cttaatacca tgaaatattt
     8221 agagacaaag gggtttgacg ttacatatct gcctgtagat gaaaaaggga atctgtcgat
     8281 cgaagaggtc aaaaaagcgt tacgagaaga tacgatcttg gtctctgtga tgtacgggaa
     8341 caatgaaatc ggaaatttga tgccaatcaa agaaatcggt gaacttttag cggatcaccc
     8401 agcttatttc catacagatg cggtacaagc gtacggaaac caaacgatcc atccagaaga
     8461 attaggaatc gatttattaa gtatctctgc tcataaaatc aatgggccaa aaggcgtcgg
     8521 tttcttatat aaacgagacg gagtaaatat ccctgcattg cttttaggcg gggagcagga
     8581 agaaaagcgg cgtgcaggta cagaaaattt agcagggatc tggggtatgg caaaagcagt
     8641 tgaactgctg tcatctgaag aacgtgaagc acgtaacaac aaatataaag gattccagca
     8701 attgattctt gatacattag aagcaaacga aatcccttat caagtaaatg gtgatttaac
     8761 gaataaactc cctcatgtgt tgaatcttcg cttaattggc gaagcaaatg atttgctact
     8821 gatgaagctt gatcttaatg gggtcgctat ttctactggt tcagcttgta cagcaggaaa
     8881 tatcgagcct tcacacgtat tagaagcaat gtatggagaa gatacaccga tactaaaaga
     8941 atccatccgt atcagctttg gtttgggaaa taccgaagac gacatccagt atttccttga
     9001 tcaattagtt cgtgctgtca aaaaataact agtgaagtca ttcttatatg aacagatgtc
     9061 tgagagaacg ccttcatttt ttagggaact taactgaaaa acctagtaaa aatcaggata
     9121 aacttttata ataaagagaa actaatttta tcgaggtgaa aatcatggca tttgaaacaa
     9181 cagcaacggt actgggcgca ccaattagct atcgtttgca tcctgatgca aaaagatata
     9241 cattgcgcga taatggattt acagaaacaa acggaggcaa ttatcaattg atccgaccat
     9301 tagatgccac gccgcaaagc aaagaaggat tcaaattaaa aatcactgta tcaaaagata
     9361 ttcaaacatt gaaaatgtcg atcactacag caaatggatt gaaagcagtc aatattttca
     9421 aagatccaaa acagaaaatg agccaagata aattttattt tctgatggat gggatgatta
     9481 gccgtgggtt attcgaaaaa gtcgaatgat ctgtggatat aaaaagacaa ctagttttga
     9541 aacggcctat tgttagatga ctaactttgt aaggacgacg caaattagtt gtctttttta
     9601 tttttaaatt taacacatgt tgttattttt aatattttgc tgatttattg tttctaagta
     9661 aagaaattta aattgaaacg attgttattt agggaaaaag gtattttttg aaaaaagaat
     9721 cccgtataat gattgttgtg caaacgctat acaaaatgaa aaagaaaaga gggatgttta
     9781 tggaaaatga acagtcggtc gttttaacga attggaagag aaattatttg ttctttttat
     9841 cgggacagtt tttgtcagga atcactagta tggtcgtcca atatgcgatc atctggtatt
     9901 tgacaagaga aaccggttcg gcgacaattt tgagttttgc tactttacta ggaatgattc
     9961 ccatggtttt actaagccca tttgtaggac cgctagtgga tcgctgggat aaaaaagctc
    10021 ttttgattgt cacggatatt attgtagcga ttttcgcgct tattttagca gtagtcggaa
    10081 cgatttcaga atccttcccg atttggctag tttttgtgtc tttgtttatg cgttcggtcg
    10141 cacaaacatt ccaaatgcca acgatccagt cgattatgcc gacaatcgtt ccatcttctc
    10201 acataacaag gacgaatgga cagttaggaa tggtccagtc agcgaatttc atcattgcgc
    10261 cagctctagg tgcggctttg ttttcagtcg ttcctgtcaa ttaccttatt ttattagatg
    10321 tattgggggc agtttttggt gtcggcttat tgatttttgt gaagatccca aaagtttctc
    10381 ctgaaatatt ggaagttccg cttactattt ttaaagatgc aaaatttggg ctgcagcaat
    10441 tgatggataa caaagggtta tggtacataa cgatcaatgg tgcatttgtg atgctgttgt
    10501 tcatgccagc aatcagtctg tatccattga tgacgctaga ttactttggt ggttccgttg
    10561 gacaggcggg agcagtggaa gtagtctacg cagtcggaat gctcttaggc ggtgcactga
    10621 tcagttttat aggaacatgg aaagaccgaa tgaaacctat tattatagcc tacatcatta
    10681 tgggcctgac gatcggtgca agcggattgg ttccaaacga tagtcaaggg ttcttgtact
    10741 ttcttatctt gaatgcagga gcaggttgtg caacgccata tttcaataca ctgctgatgg
    10801 caatgatcca gcagagttat gaatcgaatg ttttaggtcg tgtcttaggg aacttcaact
    10861 ctttgatgaa cctggcaggt ccaattggat tattatttgc aggaccatta gctgatcgtt
    10921 taggagtaga aaaaatgttc ttgttttctg gaatcgggat tttattatgc ggaatcgttt
    10981 tatttttaac gtcagctgct cgaaattatg ataaagaatt acagaaaaaa ttggtaaaag
    11041 aacaccatga gcaaaaagac gaatagtatg gaatggagtt gtgacagaag tcttgttaca
    11101 actccatttc tgatcctaat gttcctgtgg caataagcct actcaaatga aaaatatgag
    11161 taattatctt tatttgttta atacttgata tagggcagtt ttttcacgac ctatcgtact
    11221 tcctctttgc ttcatagatt tctttgattt gttgctcaga taacggagtg ccaaatggta
    11281 gtttttcctt taagagttct ctttccggtg cgtctacgcc tacattgaca tcgttctcga
    11341 taggtacgct gtaatgatgg atatgtccat gaagattgcg gatagtgtcg ttaggcccga
    11401 gcaacatcgg ataatgagtc aagtaatatt gttgatggtc gaatttaacg atggcaccta
    11461 catcataaaa ataaaatttg ggacttccgt cagtaagcct aggatcattt ttttctaaat
    11521 aacggaaaaa agcacgactg tcatggttcc ctttgatcaa gtggattgtt ccttgcagtt
    11581 gagtgatcag ttccaaaatt tctgcatttg ctttttcata ttgcggatgc atagcaaggt
    11641 cgcctagatg ataaacatgg tcggtttctt ttattactgc attccagcta gtgatcaact
    11701 gttggttcat ttcatccaca tttgcaaaga gccgcggagc aaagtcgttt tttcctaata
    11761 agcgttcatg gaaaaagtgc atatcactgg taaagtaatt catgtatctc ttgcctccct
    11821 tttttcttag agtaaacaaa atcacttatc tgagcaactc ataacatcta aaaagaaaag
    11881 gttgtaaatg agacgtgcag ctttaagcga taagaagcaa ttttgaaaaa tagtttttca
    11941 caatttacaa aattattcct tattgccgaa aggctggccc tttttatgag tatgccaatt
    12001 ttttatatcg aatgctcaaa agatgagtga ttttttggtt gactatcatg tttgtgtact
    12061 gaataggtcc agtagaacag tatttttacg acttgataat taagggatga aattttcaga
    12121 aatatgacgt ataatgtaaa atactgaaaa gttgcgacct tcaactcgta gatgtttcag
    12181 aatctaattg aaatgatggt gatatcatga cagacaacag caaaacacgc gttgtcgtag
    12241 ggatgagcgg cggtgtcgac tcatctgtaa cagcactttt gttaaaggaa caaggatacg
    12301 acgtgatcgg aatattcatg aaaaattggg atgacacgga cgaaaacggt gtatgcacgg
    12361 ccacggaaga ttataaggat gtagcgaaag tagctgctca aatcgggatt ccttattact
    12421 ctgtgaattt tgaaaaagaa tattgggatc gtgtatttga atatttccta gccgaatacc
    12481 gcgcaggacg tacacctaac ccagacgtga tgtgcaacaa agaaatcaaa ttcaaagcat
    12541 tcttggatta tgcaatggat cttggcgccg attatgtagc tactggacat tatgcgcaag
    12601 tcactcgcga tgaaaacggc gtggtccaca tgcttcgtgg agtagacaat aataaagatc
    12661 aaacttactt ccttagccaa ttatcacaag aacagctttc aaaaacgatg ttccctctag
    12721 gcggcatgga aaaatcagaa gtacgggcaa ttgctgaacg cgcaggcctt gcgactgcta
    12781 agaaaaaaga ttcaacggga atctgcttta ttggtgaaaa gaacttcaaa caatttttga
    12841 gcaactatct gccagcaaaa aaaggagata tggtcactct tgatggtgaa gtcaaaggac
    12901 aacatgctgg cttgatgtat tatacgattg gtcaacgtca aggactaggg atcggtggcg
    12961 gaggagattc gcaagaaccc tggtttgttg taggaaaaga tctagcgaca aacacattat
    13021 atgtggggca agggttccat catcctgcat tgtatgccac aagcttggat gcaagtgaaa
    13081 tccactttac gacgaatgaa ccaatgccaa aagaattcaa gtgtacagcg aaattccgtt
    13141 atcgtcagca agacgttcct gtgacagttc gcttattgga tgataatcgt gcagaagtcg
    13201 tattcgacga acctgtacgt gcaatcactc ctggacaagc agttgtattt tatgatggaa
    13261 tggaatgtct gggtggtggt ttgatcgacc acgcatatca agaaacaaaa gttttacaat
    13321 acgtttaagc gatattaaat gaatcaaaac agctatggct aacaaaagaa acaccttttc
    13381 ttttggatag catagctgtt tttttgcaat aaaattaatt tttattcatt ttatacttga
    13441 aatacttgtg taaaaagttc ataatatcca ataatatgaa taaagttgct ttttgttaag
    13501 caagagcagt tgcaaaatag aagggaatct tttgcaagtg atttgttcaa ttttataagt
    13561 gagggtgatg gaaatggaaa agaaatcgac gggtattagc aaatggggac tggttgctct
    13621 ggttgtcagt tcgtcaatcg gaagcggtgt gtttgggatc acaagtgatc tggctagttc
    13681 tgcagctcca gggccagcga tcctttcatg gataatcgtt gggattggta ttttagcttt
    13741 ggtattatcg ctgaatcatt taggagaaaa acgtccagat ctagatggtg gtattttcgg
    13801 ttacgcggaa gcttcatttg gtaaattagg tggcttcatc agtggttggg gttactggtt
    13861 gtctgcgtgg ctgggaaatg ttgcctttgc aacgatgctg atgagcgcca tgggagaatt
    13921 tttcccgata ttcaaaggtg gacaaaatgt tccttctatt ttatttgcaa gtatttttat
    13981 ttggggtctg actttattag taaacaatgg agttgaagga gcgagcgtca tcaatacaat
    14041 cgtgacgatc tgtaaattag tgccattatt cctattttta atatttgtat ttatcgcatt
    14101 taaagtgaat atattcactg ctgatttttg gggtaacgta tctgataatc tagtgaatgg
    14161 aacagggcag caaggaacga tctggttgca gattaaaggt tgtttgatgg ttatgatgtg
    14221 ggtctttgta gggatcgaag gcgcaagtgt tttagccaac cgcgcgaaga aacgttcaga
    14281 agcccagcaa gcatcgatca ttggtctgct ttgtttattg tttatctata tccttgcttc
    14341 gatcttgcca tacggcgtct tatctcaaga agagttagca actatttcac aacctgctat
    14401 ggcaaatatt ttaaaaggaa tcgtgggaaa ttggggagca gcgctgatca atatcggctt
    14461 gattatttcg atcattggtt cttggttgtc ttggacgatg ctcccagcag aaacaacgat
    14521 gttgatggct cgagacggtc tgttgccgaa aggttgggga aaaacgaata aaaaaaatgc
    14581 cccaacttat tcgttagttg ttactgcaat cttgactaat ctattcttat tgactttctt
    14641 agttactgat tatgcctatc aatttgccta ttctttatgt acagcggcaa ttttgatttg
    14701 ttatctactt gtcgggatct atcaagtcca gttttcttta aaagaaggaa tcaaaaaaca
    14761 agtagtgatc ggtgggattg ctgtagtttt tgaattgatg ggcatcattt tagcaggatt
    14821 ttcttatgtc cttttatgta gtattgccta tcttcctggt ttttgcttct actggatcgc
    14881 ttgtcgtgaa accaaacatc aaattacggg aaaagaaaaa gtaatgattg ggctgatttg
    14941 tgtggccgca ctgctttcaa tcgggttgct tgctgtaggc tggatcaaga tataaggggg
    15001 aaagaaacat gaaaatcgca aatttcggtg tagaagaatg gctaaataaa tgggaaaaga
    15061 aagcggtata tgatattgca caaagctcga tcgaagcatt gacattagac gaactcgttg
    15121 gcttagatgg gactagcgta agtgaatttt tcgaaaaacg caaaaacgaa ccattggact
    15181 atggctggat cgaaggatca gatgttttca aacaagaagt cgcttctctt tatcaaaccg
    15241 tcaatccgga aaacatctta caaacgaatg gagcgactgg tgctaatctt ttagctctat
    15301 acgcgttagt ggaagcagga gaccatgttg tttcgatgct tcctacttat cagcaattat
    15361 atgacatccc caaatcttta ggtgccgctg ttgactttgt tcatttgaag gaagaagaag
    15421 attggcagtt tgatttagaa cagctagaag ccttagtcaa acctgaaaca aaaatcatct
    15481 gtttgaatag tgcaaacaat cctacaggca cattgctaga tcggaaaact ttagaaaaaa
    15541 tcgcagaaat tgctcgtaca gtagatgcat atgtattgat agatgaagta tatgcgcctt
    15601 taacggataa aggtgaattc ttgtcgatcg tagatgtcta tgaaaaaggt atagcaacca
    15661 attccctatc caaaacctac tccattccag gtattcgtat tggctggaca gctactggac
    15721 ctgagttggc agaagttttt cgaaagtatc gagactacac gatgatttgc ggcggagtcc
    15781 tgtcagatga tcttgctgta catgcattga agaataaaga aaaaatattg gagcgaaaca
    15841 aaaagatcat tacagagaac cttgtgattt taaaacaatg ggtagccaat gaaccgaaag
    15901 ttgaacttgt cgctcccaat tacgtctcta cttctttcat taaattagcc atcgaagaag
    15961 atgaccagac attttgtatc aatttattag aagaaacagg agtactatta gtaccaggaa
    16021 gtgcctttga tttaccgaag catgcccgac taggttactg ttgtaaaaaa gaaaccttgg
    16081 aaaagggatt attcctctta tctgagttct tgaaaaaaca ggcctaaaca aaaacactgc
    16141 aaggacagtc tgtgacaact gttcaagcag tgtttttcat ttacgcttat tgtctatgag
    16201 aaggagagtg ccctgaagat atggagactc ctgaagcttt ttcttctgca gtttcttgct
    16261 gatggatttc ttgtgtattg tgtcgtacaa gcaagaagtt gaccaaaaca agaagagaag
    16321 tcgaaagaaa aacgccacga taaccaaatg cactggaaac actggagccg atcataggcc
    16381 ctactacgtt accagttgct tggaaagatt ggttataact gaagatccgt ccagcagctt
    16441 gatgaggcga atattttgtg atcaatgctt ggacagcagg gagcagacaa gcatcagaga
    16501 taccaatcaa aaaacgtaag gcagctaatt gccaaacatt cgtgacgaat gacattggga
    16561 tataaaccag aatcgcaaaa cccaaaccaa tcgcaagtat tctttcactc ccgatcttgt
    16621 cacctaagcg accaaatctt ggagcggcta tcaaagtggc aatcccaggt atcgaagcga
    16681 tgattccgct gatcaacgta acattcccat gtccatgcag caattggcga atgtacagac
    16741 tgatgattgg actaatggaa ttgttggatg cttggatgat catcgttgtg acgaacatac
    16801 cgatgacgac gtgtggatat ttcaagtctt tgaaaatctg tttggcagaa accatgtttt
    16861 cttttttcac tgggacaaat ttttcatgga cgaaaaacag actcaacaag aagacgaaaa
    16921 ataagatgat ccctgtaatg aaaaaggtag ggcgatagcc aaacgctgaa gcagagatcc
    16981 cgccaagaag cggtccaagt aatgttcctg taacagaacc agttgctaag gttccaagaa
    17041 cttgaccact tttttctttt ggcgtcccag ttgcgacaag ggcagtggca ttactgatat
    17101 aaccagaaaa aatcccttga agcagacgca atgcgactaa ttgatacaca ctagtcacgc
    17161 aacccatcaa agaaatgacg attgccattc ctaatgaagc acgcaacagc atcaattttc
    17221 ttcctttttg gtcggctaat tttccccacc aaggagagat cagcgtagtc accaagaaag
    17281 tagatgaaaa agtcaatccg ctccaaaagt tcaattgtga cgtactataa ttacctaacg
    17341 tatctatgta aagtgacata aaaggcatga ctaaactaaa tccgatacca gccataaatg
    17401 ttccaaacca taagacaaac aagttttgtt cccaagtttc tcttttccta aataattttt
    17461 cctttattct tgcagccaat tagactcctt ctttctgaaa attttcatct acagatcttt
    17521 attataaact acaatatgta gtttatcaat gaaagtctct tttttctctt tctttttcgt
    17581 ggtaaactga aagagtgaag gatttatttt atgagaggaa acatggaaat gaaaaaaaga
    17641 aatctgtctc aagagaaaat catcgactgc tttcgagaac tagcagaaga gatggatgtt
    17701 cagcaaataa cctttcagca tttagccaaa cgcttggata tcaaggctcc ctcgctgtat
    17761 aaccatttca aaaatattcg tgatgtcaaa acagcgctaa cagcgaaact tctgcaggaa
    17821 cttaatgacc agctgcgtcg ggctcttgta ggaaaaagtg gtcaggaagc actgcagatc
    17881 tatgcagaaa cttatcgatc ctttgctttt tcaaatcaag cagtgtatga gttactgatc
    17941 agtgtaccgc ataccaatga agctgtttta ttagatggaa tccatgaaac gaatcagatc
    18001 attttgcaga tatttgaggc ttttcaatta acgaaagaag aaaaagttca tcgaagcagg
    18061 gaacttcgta gtatgatcca cggttatctt tccttacgtt ttttaggata ctttacaaaa
    18121 gaaccaacaa tcgatccaga agacagctat ttttggatga tcaatgactt tatcgctact
    18181 ttgccttaaa caatcagatt catttgtaac aattttaaga tgttcattaa atgacccttt
    18241 tataagagaa aattcttgca actttcttga aaagtgacta agatgagtct agaaaagctg
    18301 aaagaaggag cacgagtatg tatcaagtaa ttaccatgtt cggggacaat gagccttggt
    18361 ggttttttga agactgggaa gaagatattg aagcagaaga aacattcgag tctttcgaag
    18421 aagcacgaca agcgtacgaa aaacaatgga acgaaatcca tcaaaattat gaatatatca
    18481 atgcaaaaag taactttctc agcgcctttt ggaatgacgg agatgagcgc tggtgtgaag
    18541 agtgtgacga tgatttacag caatacaaag gacttgcgtt gttgaaaaat catcagccca
    18601 tcacagtaga aagcggaaag gaattttatg aaacaactaa ttctagcgga aaaaccaagc
    18661 gttgcgaaag acctaagcaa ggtgcttggt gctaatcaaa agcacaaaaa ttattatgaa
    18721 ggacccaagg tgatcgttac ttgggccctt ggtcatttat taggattgaa aatgccggaa
    18781 gatctgaata aggaatggca gagctggcaa atggacacgt tgccaatgat ccctaaaaaa
    18841 ctaggaatca aaccacttcc taaaacagga catcaattaa aagcaatcaa gcagctagct
    18901 cagcgaaaag atgtctcaga agcagtcatt gcgacagatg ccggaagaga aggggaactt
    18961 gttgctcgct ggatcttaga gtatgtgcat ttcaataaac cagtgaagcg gttatggatc
    19021 tcttctcaaa cagataaagc aatcaaaact ggattcaagc aactaagacc tgcgaaagaa
    19081 tatgatgcat tgtatgaatc tgctcttgcc cgggcaaaag cggactggct ggtaggattg
    19141 aatgtgacca gagccttaac tgtgaagtac gcggataatt tatcagcagg ccgtgtgcag
    19201 acaccgacac tagcattagt gagagaccaa gaaaagaaaa tcgagcagtt ccgtccacaa
    19261 acgtatttca cgatcacgtt gcaagctgga aaagaacaag ccaaaatgct tcaaaaaaat
    19321 caatttgctt taaaaacaca agaagaagca caaaaacttg ttcaattgat cagtacaagc
    19381 aaaggaactg tcacagaagt ccaagaaaaa ttaaaaacgg aatctgcgcc attgccttat
    19441 gatttaacag agatccaaag agaagccaat caacgctatg gctattctgc aaagaaaaca
    19501 ttaaatcttg tacaaagttt gtatgagacc cataagatcg ttacttatcc gcgtacagac
    19561 agcaaatatc tgacaaatga catgaaaagt acgatgaaag aacgattgca agcagtcagc
    19621 gactttgctc ctgaggtcaa gcagtatttg aaaaacggag ccatcgtcca acagacaaaa
    19681 gtattccaag acagtaaagt gaccgatcac catgcacttt tgccaactga aaacagagca
    19741 cgttatgaaa aattaagtaa tgaagagcaa aaaatctatc aaatgattgt ctctcgtttt
    19801 ttaggactgt ttgctcaacc ccataaagtc agccaaacaa aagttacggt tgaatttggt
    19861 aaagaacgat tcatttttcg gcaaaatcgt gtcgtccaag caggatggaa aggcgaaact
    19921 gaaactgaga cggaaacagt caaatgggaa aaaggaatga gaatcaatcc cgatttcgcc
    19981 atcaaaaaag aactatctgc accgcctaaa ccattgtcag aagcaagctt gcttggtcaa
    20041 atggaaaaat acagtctggg aaccccagct acgcgtgcag agattatcga gaaactaatc
    20101 aaatctgaac tgatggaacg tatccctcaa ggactgcaag tatctcctaa aggtaagcaa
    20161 ttattagtat tggtaaatcc ctctttagta acaccagatt tgacagaaag ctgggaaaaa
    20221 gaccttgaag ctattgctgc aggaaagaaa caagcaggaa ccttcttgaa tggaatcgaa
    20281 aaagaaacaa aacgcttggt gaatgagatc aaatcaagca aacaagaata tcaagatttc
    20341 tcgattaccc aaaagaaatg cccagaatgt ggggccaatc tgcgcgagaa aaacacacgt
    20401 gacggcaaaa tctatgtctg caccaattca gattgcaact accgcagaag aaaagatcca
    20461 aaagtctcta atcatcgatg cccgcaatgt caccggaaaa tggaaatcat cgaaggtaaa
    20521 aacggtgctt atttccgttg taaatacgat ggaacgacag aaaaaatgct tgataaaaaa
    20581 gagcagaaga agaaaatgac caaacatgaa gaacgacgct tgctgaaaaa atatgcacaa
    20641 gcagaagaac cagaagaaag cccattagca gcagcactaa aagcggcaat gaagaaataa
    20701 taagatatga aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaatttac
    20761 gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt tccgaagaag ctacttctgg
    20821 aacaccgttt accaagaggt cgtgaaaaag ccgcttagtt tcgagtatta agaaaaattt
    20881 tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt
    20941 tgaacaccgt ttattcgaaa caagaggtcg tgacaagact tttgtcacga cctcttgttc
    21001 ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt atttattgac
    21061 gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa aggattcagc
    21121 ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct tcagcaggct
    21181 tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat tgggtatttt
    21241 attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat ttgctgaaag
    21301 caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc aaacgattgc
    21361 tcaaattggt ataaaaggaa ttgtttttgt gttactgcaa atgtcagtca ctatcatcgg
    21421 tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga tgatggctgg
    21481 aggaaacgct gtttgtggtt cttctgctat tgcttcgatc gccccttcca tccatgcgaa
    21541 agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg tgatgatgtt
    21601 gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa aaagtgcact
    21661 tttgggtggc acactccaat ctgttggtca agtagtcgct ggggcaagct tacttgacaa
    21721 ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc tcgttgttgt
    21781 tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg aaacgccaac
    21841 tgatacgcag aaaaaacgaa aattaccact tccttggtat gtattagggt ttgtgatctt
    21901 ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg cacattttat
    21961 cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag actttgcaaa
    22021 attcatgaaa gaaggtacac gcttcttatt ttatggatta gctgtcgggc ttctccaaac
    22081 agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg aaaaaacagt
    22141 tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg cagatatcct
    22201 tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc catatatcct
    22261 gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg caatggattg
    22321 gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc aacggctact
    22381 tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca gacattttgc
    22441 tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct ttttcccgca
    22501 aaatattctt caaactgttc ttgtgtgcgc gttaaaattt gctgattcgc tgattcgctt
    22561 tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc taaccataaa
    22621 aagccgatag gtgtgtgtat tttcattttg tttgcttcta gtcaatagac tggaacttcc
    22681 tcctttttct ctatccaaaa ctacttgcca actaataaaa attgttctta aaagtttgat
    22741 aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac ctgttctttt
    22801 acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa tgtaaaccat
    22861 atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg tcccatgttt
    22921 taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca aaggtgccca
    22981 tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag cagcaatcaa
    23041 gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa attttgggaa
    23101 agaagcggct ctatacgctg gattgcaagc ttctacagga gatttagtga ccgtgatgga
    23161 tgttgatctg caagatccgc ccgaattgct tcctgagatg atcaaccgga tcactacttc
    23221 tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg cgatacgcag
    23281 tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag aaatggtcga
    23341 tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc tagaattaac
    23401 agaatacaac cgattttcaa aaggattatt tagctgggtc gggtttaaaa cagaatatat
    23461 ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct ggaaattatt
    23521 caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga ttgcttcatt
    23581 tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta ttttgcgggc
    23641 attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct tcctgttcat
    23701 tggtggaatc caattgttat gtcttggaat catcgggaaa tatatcggga aaatcttctt
    23761 agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga agccaaatga
    23821 attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa atgaaaaagg
    23881 actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc cagaagtagc
    23941 ttcttcggaa ataagccgaa atttcacaaa aatttgagaa g
//
LOCUS       DAEGIQ010000036        23908 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000036 DAEGIQ010000000
VERSION     DAEGIQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.036"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            135..2744
                     /gene="clpB"
                     /locus_tag="KMT45_001292"
     CDS             135..2744
                     /gene="clpB"
                     /locus_tag="KMT45_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBE7004763.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2895..3581)
                     /locus_tag="KMT45_001293"
     CDS             complement(2895..3581)
                     /locus_tag="KMT45_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004764.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3559..4053)
                     /locus_tag="KMT45_001294"
     CDS             complement(3559..4053)
                     /locus_tag="KMT45_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBE7004765.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4073..5293)
                     /locus_tag="KMT45_001295"
     CDS             complement(4073..5293)
                     /locus_tag="KMT45_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7004766.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5440..7275
                     /gene="lepA"
                     /locus_tag="KMT45_001296"
     CDS             5440..7275
                     /gene="lepA"
                     /locus_tag="KMT45_001296"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBE7004767.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7471..8382
                     /locus_tag="KMT45_001297"
     CDS             7471..8382
                     /locus_tag="KMT45_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7004768.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9378..9629
                     /locus_tag="KMT45_001298"
     CDS             9378..9629
                     /locus_tag="KMT45_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004769.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9712..9984
                     /locus_tag="KMT45_001299"
     CDS             9712..9984
                     /locus_tag="KMT45_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004770.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            10074..10313
                     /locus_tag="KMT45_001300"
     CDS             10074..10313
                     /locus_tag="KMT45_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004771.1"
                     /translation="MIDITLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10460..14743
                     /locus_tag="KMT45_001301"
     CDS             10460..14743
                     /locus_tag="KMT45_001301"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBE7004772.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14842..15207
                     /locus_tag="KMT45_001302"
     CDS             14842..15207
                     /locus_tag="KMT45_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004773.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15209..15661
                     /locus_tag="KMT45_001303"
     CDS             15209..15661
                     /locus_tag="KMT45_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004774.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16275..17585
                     /locus_tag="KMT45_001304"
     CDS             16275..17585
                     /locus_tag="KMT45_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBE7004775.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17608..17919
                     /locus_tag="KMT45_001305"
     CDS             17608..17919
                     /locus_tag="KMT45_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004776.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18280..19536
                     /locus_tag="KMT45_001306"
     CDS             18280..19536
                     /locus_tag="KMT45_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBE7004777.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19533..20078
                     /locus_tag="KMT45_001307"
     CDS             19533..20078
                     /locus_tag="KMT45_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004778.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            20079..20579
                     /locus_tag="KMT45_001308"
     CDS             20079..20579
                     /locus_tag="KMT45_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004779.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20660..21216
                     /locus_tag="KMT45_001309"
                     /pseudo
     CDS             20660..21216
                     /locus_tag="KMT45_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21236..21580
                     /locus_tag="KMT45_001310"
     CDS             21236..21580
                     /locus_tag="KMT45_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004780.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21599..22510
                     /locus_tag="KMT45_001311"
     CDS             21599..22510
                     /locus_tag="KMT45_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBE7004781.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22589..23581
                     /locus_tag="KMT45_001312"
     CDS             22589..23581
                     /locus_tag="KMT45_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBE7004782.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            23578..23796
                     /locus_tag="KMT45_001313"
     CDS             23578..23796
                     /locus_tag="KMT45_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004783.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
BASE COUNT         8264 a         3795 c         5031 g         6818 t
ORIGIN      
        1 atttatataa gtcgagaaaa aaagtcaaaa aactatgtgt atattttgac caaaattgac
       61 caaagcgata taatagtttt tgtaaagatc aaagaaataa gtgatcttac ctttaattag
      121 gagggtatac ctgtatgaat attgagaaaa tgactacgac gcttcaagaa gcgattgctg
      181 aagctcaaca gattgcggtc actcgaaaac atcaagatat cgacattgcc catgtatgga
      241 aaatcttttt acaatctaat cattttggtc gaaatttcta tacggatgcc ggattggatg
      301 tcgaatcttt tgaacatgaa attgatcgtc tactggatga gtatccagtt gtttctggtg
      361 gaaacgtcca atatggacaa aacctcagcc agaatttctt tcgattattg aatgaagcag
      421 accaaatacg tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt attttagcgt
      481 tgatgaaatt aaaaaattat ccgttaactg tttatttgaa taaaaacgga ttatctgaaa
      541 aagaattgcg taaaaacatc gaagagatga gaggaggaga aagagtgacc tcacaaaacc
      601 aagaagaaca atacaaagca ttagaaaaat acggcgttga tttagtacag caagtcaaaa
      661 gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc atccgtattc
      721 tttcacgaaa aacaaaaaat aatcctgttc tgataggaga acccggtgta gggaaaacgg
      781 caatcgttga agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa aatttaaaag
      841 ataaaacgat tttttcatta gatatgggtg cattgatcgc tggagcaaaa ttccgtggtg
      901 aatttgaaga aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat ggacgtatcc
      961 tcttgttcat cgatgaaatc cacaatatcg taggggcagg aaaaacagaa ggaagtatgg
     1021 atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga attacatttg atcggtgcca
     1081 caactttaga tgagtaccgt caatatatgg aaaaagacaa agcgttggaa cgtcgctttc
     1141 aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat cagtattctg cgtggattaa
     1201 aagaacgttt tgagatccac catggtgtta acatccatga caacgcatta gtagcagcag
     1261 ctactttatc tgaccgttat attactgatc ggttcttacc ggacaaagca atcgatttga
     1321 tcgatgaagc cagtgcaacg attcgggtag aaatgaactc tatgccaacg gaattagatc
     1381 aagttactag acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa aaagaatcag
     1441 atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa cttcgtgaag
     1501 aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat tctgtttcag
     1561 caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga agatgcagaa aataactatg
     1621 atttagaacg cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa aaagaattga
     1681 aggaattaga agctaaagca aaagacagcg agattaagat ggttcaagag tccgttaccg
     1741 aaaatgagat tgctcaagtc gttggcagat taacgggtat ccccgtaacg aaacttgtag
     1801 aaggtgaacg agaaaaacta attaaattga atgaaacact tcataaacga gtgattggtc
     1861 aagatgaagc agttgatgcg gtcagtgacg cggtaattcg ttcacgggct ggattgcaag
     1921 acccaaatcg tcctctaggt tctttccttt tcctaggacc aactggtgtc ggaaaaaccg
     1981 aattagcgaa agcgttagct gagaacttgt ttgactcaga agatcatatg gtacggatcg
     2041 atatgagtga atatatggaa aaacatgctg tgtctcgtct agtcggagct cctccaggct
     2101 atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat ccttatacaa
     2161 tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc ttgttacaag
     2221 tcctagatga cggacgcttg actgattcaa aagggcgagt agtagatttc aaaaatacgg
     2281 ttctgatcat gaccagcaat atcggttcac agttactgct ggaaggcgtg actgctgatg
     2341 gaacgattcc agaagcagtg gctgaacaag tcaacacgct attacgtgga aacttcaaac
     2401 cagaattctt gaaccgaatc gacgacacga ttttatttac accattgagc ctagacaacg
     2461 tgaaaggaat cgttgacaaa atggtggcgc aattggcaca acgtctggaa catcaagaaa
     2521 tcctgctgac aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct tatgaaccag
     2581 catatggtgc aagaccattg aaacgattta tcactaagga agtagaaaca ccactagcta
     2641 aagaaatcgt tgcaggacat gttatgccta aatcaaaagt aacgattact ttacttgatg
     2701 gacaacttca tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc attgagacta
     2761 taaaagaaaa aaggggtctg acacaactag tgtcagacct ccttttttca aataaacagt
     2821 gttccagaag tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc ccgcttctgt
     2881 cacaacctct tttattaggt atttttcact ttttctaatt tataactttc aaagtaacgc
     2941 tcatcttttt tactttgaga ccatgcaaac aatcccatta aggcagcacc tatcgtattt
     3001 gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc cgacaaacag tgtgccatca
     3061 gaagcagcaa aacgttgaag attcatgcct aagaactggt cgcataagaa ttcccagaac
     3121 tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga ataagaggaa caaccaagga
     3181 ctggtttttg atatctcagc atctttcatc aagtaaccaa tcaatccata gccaagagct
     3241 gtcaggacca ttggactcac cgcatgtaag attttgtccc aaaaagagat gatactgatc
     3301 aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga aaaaccagta aaacaaaacg
     3361 attgctgctg gtattttaag tttgaaaact ttcaaaatga cttgtggcat gaaaatcaat
     3421 gcaatcccta ataaagattc cacgataaat gtgattcctt gagaagtcga atatttttgt
     3481 gtgataaatt cataaaaatt atagcagaca acgaatacac caaaagcaag taaagctatt
     3541 tgcacctttt tgatttcttt aatcactgaa agtccctcca tctaaaaatc tttttacttc
     3601 tgaaagcaac tcaatttctg aagaaaccac ttttccgttc aatttgatca aacctattgt
     3661 atataagttc aggtagtgaa attgattttc agctgtttct tgtagtgcag cgattttttt
     3721 cgggtgggtt ccgccttgct gtcgagtatc agtatataag ccgatcactg ggatgccttt
     3781 cgcataagcc acaccgattt ccgatgctac cccggcatcg atcgtcagcc catctagtaa
     3841 agcaatcatt aaatcactag ccaacacttt ttctgtgtca gcaagagcaa tcattcgact
     3901 gtctgcataa gcagttttgt cattgattgc ttcattttct tgaggaagat agatcgaaag
     3961 ctcagaagag agctctctga tttctttaac taactgttgg ttatataaca aatcactttt
     4021 agcaaacatt ggtgcggcaa aataaatttt catctaaaca tcatcctttt tatcaaaaat
     4081 aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc tagtgacttc agtgtttctt
     4141 catctgtttc taaccgttcg attcttgcca aataagaagc acggcgataa ttcatcatca
     4201 agcaagtcaa tgtttggata tccagtcgaa cggattttcc tataggttca tttaagacac
     4261 ggacttgtcc ctgttcatcc catgttaaag cgaagacacc attattccaa ggagcgacag
     4321 gatcttcaat gatgaaatga aaagcatcag ctgttccaac aaacggaaac cgttgaagga
     4381 attcttttac atcaacgatc cgagccataa aatatggttc gatctgctct ttgatctgac
     4441 tatcttcaat caaaaatgct aaaggctcat ttttataaat atctccatgt acccagtaga
     4501 tcatggaaaa atgtgcggaa ataaagttcc ataagccatt tcgagcttct tgatcaagat
     4561 agaacatttc ttttacgtga aatatttctt cagctaccca gtagaacaga acacctcttg
     4621 gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc atcttcattt tcgaaacgcc
     4681 aatactcttc ccaatgaaaa tcactgcgga ataaagcacc atggttttgt tgggcaaatt
     4741 tgtcataaac ggaaaatacg tctggatgat caactggtaa tcgctcaacc ataccactta
     4801 ctgggatttg tttaggtagc tgtgtatctc tgattttaaa ggaaagttta tcagacatga
     4861 tttcccatcc tttacgccga tagtagggga tactgtaagg atacaaataa gagatccatt
     4921 gcttattctt gcgcatatta tccaatgcaa cgataattag atcttgcatc aatccatggt
     4981 tagcatattc tggataagtt ccaactccag tcacaccacc cattttataa cgtgcaccgt
     5041 gaatattgac ttcacaagga tagatagcga tttgagagat cagtttattt tcgtggaacc
     5101 atccgaacac tttcgatact tccaaaatcg gttttttgga acggataaaa gctcgtttat
     5161 tctcaaaccc gctttcctcg atgtctgctt cagtcacttg gaacacatag gaaagcaatt
     5221 cattgaattg atcgatatgt tcttgagtga ccggttttaa ttcaagattt tctctaaaat
     5281 ctttttgatc cattttttca tctccattta ttagcttgat aacagtatag caaaattcta
     5341 caaaaaaaac ttctatagct gactaattaa aaactcttta aattactgct tttcgttggt
     5401 ataatggaaa gtgctgatta tatgaaaaga ggagacgcta tggatataaa gaaaatgaaa
     5461 gaacgccaag agcagattcg taatttttcg atcatcgctc atatcgacca tgggaaatct
     5521 acattggccg atcgtatttt agaaatgacg cacactgtga cgtctcgcga aatgcaagat
     5581 cagctgttag actcgatgga tttggaacgt gaacgtggaa tcacgattaa attaaatgct
     5641 gtagaactca attacacagc gaaaaatgga gaaacatata tctttcactt gattgacacc
     5701 ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg tgagggtgcc
     5761 gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa cattggccaa tgtttattta
     5821 gcgttagaca atgatttgga gatcctgcca gtcatcaata aaatcgacct acccgcagca
     5881 gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc tagtgaagct
     5941 gtattagcca gtgccaaatc agggattggg attgaagata ttctcgagca gatcgtggaa
     6001 tatgttcctg caccaagtgg agacttagat gcaccgctaa aagctttgat ctttgactct
     6061 atttatgata gttaccgcgg tgttgttcta aatgtccgga tcacggacgg tatggtcaaa
     6121 cccggagaca aaatcaaact gatgagtaat ggcaaaacat ttgatgttac agaggtcggg
     6181 gtcttctcgc caaaagcagt tgctagagac tttttgatgg tcggtgatgt cggttatatc
     6241 acagcaagta tcaaaactgt ccaagataca cgagtagggg atacagtaac gttagcagac
     6301 aatcctgccg ctgaagcttt accaggttac cgaaaaatga atccgatggt ttattgtggt
     6361 ctttatccaa ttgatacttc tcgttacaac gatttgcggg aagctttaga aaaactgcaa
     6421 ttgaatgatg ctgctttaca attcgaacca gaaacctctc aagcattagg ttttgggttc
     6481 cgctgtggat tcctcggact tcttcatatg gatgtcgtcc aagaacgctt ggaacgggaa
     6541 ttcaatctag aattgatcac gactgcacca tccgttattt accatgttaa taaaacggat
     6601 ggtacgactg ctacagtaga taacccggca gattttcctg aacctgtaac gattcaggat
     6661 gtagaagaac cattcgtcaa agcacagatc atggtcccaa atgattttgt cggtgcagta
     6721 atggaacttt ctcaacgaaa acggggagaa ttcattacga tggattattt agatgattac
     6781 cgagtaaatg tcgtctataa tattccgctt tctgaaattg tctttgactt tttcgacaaa
     6841 ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg aaatgtctgg ttatcaaaaa
     6901 agcaagttag tgaagatgga catcttgctg aatggagaaa aagtcgatgc attaagcttt
     6961 atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa actgaaaaaa
     7021 ctaatcccac gccaacaatt tgaggtgcca atccaagcag ctattggaca aaaaatcgtt
     7081 gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta tggtggagat
     7141 gtttctcgta aacgtaaact cttagagaaa caaaaagaag ggaagaagcg gatgaaacaa
     7201 atcggctccg tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat ggatgagcaa
     7261 gatagtgtga aataacttat aatcaatcga aaaacctatt aaataaacct ttttgagtag
     7321 atgcaacact gaggattatc tgtataaaag attttaaact gtcatttggt tatcacggcg
     7381 atactaaaat taacaaaatt ggaatatttt tcttgaagat ttttagctgt cagtatatga
     7441 tgaaagcatc tcataggagg tgcttttttc atggttgata attcaagtgg aagggttctt
     7501 aaatctctaa gaaaagagaa aaaactatct caaaaaaagt tagctgattt agcaggtatt
     7561 tcacaaagta ctttagttaa atatgaaaaa gggtcaagaa aaatccctaa agacgtagat
     7621 aatactctaa gcaagattct gaatatagaa actctaataa aagatgagga agataagatt
     7681 gagattctca tcggtaaact aattgcatac agagatatga ataagttact taacaaagag
     7741 ttagctgata atataggaat tagtgaagta ttactcagtt atgtgttaaa tagaaagaga
     7801 aatccgtcaa aagaaatgca aaaaaaaatc gatatttttc tattaagtaa tgaaaaagaa
     7861 attttgaagg agattaatcg tgatagtgag atatttagtt tatcaaaaga cgataaaatt
     7921 gttatgggaa aaagaatacg agaagtccga aagaaccggg aagagacgct tgaaaaattt
     7981 ggtaaaaact ttaccatcta tacaggcaaa aatgttataa gtagatggga aaaaggtatt
     8041 aatattccag atattgaaaa actaatgaat attgcctatc ttggtaaggt cactgttcct
     8101 taccttatgt acggagaaga ttataagaat atacttccaa aagatgaaag agttagcgat
     8161 tttaaaaaga taaattcttt tagtatggga ttaaggatga ggaaaattag gaaagattat
     8221 tatctagaaa gggaggagtt tggaaagctt ttttctcctt caatttcaaa atggtccata
     8281 gataggtatg agaatggtag agatataccc aatactaatc gaattatcca atatgcttat
     8341 ataggaaatc tttctttaga atttttgatt tacgggatat aaaaatagtt tacgtcgata
     8401 cttattaagt tatcgacgtt ttttattata gaaaaaattt ataaaaggag gtttcttgat
     8461 atgataagaa acaaaaaggg tttgttgaaa gttgctttac tggcagcttt tttctaagta
     8521 actttttgac aagctcaatg gcaacacaag cggaagaagt aatacaagaa ctaactgtta
     8581 tgaataacag ctagataaaa tgggctatgt atgttatcca acaatttagt aatcactaga
     8641 agcctagcga aagttcgctt gataagttat agatgagtat tgatttgtag tgtttccaaa
     8701 tcaaaagggt acagagccac atgaaatatg ttgtcctctg cagcgtcagg atgggaatcc
     8761 ataccatgga gattcggaga tctgtgaggg aatacttacg tggttaatat gtcagttgtg
     8821 ttggacgtag taatggtcat agtgggtaga ttgactgaat tcttatatga gaaaatgaaa
     8881 ccaacaagaa gatagggtta tttatacaga attggggatc ttttctggaa aagtaatcgg
     8941 ataatgcgag attcgtacgg tcttttttaa atttctctaa aaaaaacggc atcgaatggg
     9001 ttgggtgatt gataagcaat gaatcataga ctgatataga ataaaaaatg attctattta
     9061 tctttttaca tctatcgttt tttcaagtag ccgcaaatta tatcagcatt caacaatttt
     9121 tgtatacaag ttctagtaaa aggatctgaa gaaaaagttg gttgttgtga agataaaaaa
     9181 agaatcaggt tttactgagt ttttcttcaa aatttgtgtt attaggttag gacaaagata
     9241 agaagaagta tgaagattta aaaaaggaat gaggaaaaga tagtaatttc cgattattgg
     9301 agttttttta tttataaaga agaagcgaat aagttgtgat tcctagattt cgcgcgtgat
     9361 taattaatga gcaaaaaatg cggaaagttt tagtgtttaa tacacgagga caaagtttct
     9421 taggtattga aggcacacgt gtaacggttg atgggtggtg tgattgtttc gaggttggaa
     9481 aacatgttga acttatggca tttactgagc taaaagataa gaacaacgtg gaatttttcg
     9541 agagtgatat tggttgggac gatcatgaag aagtttatgg aaaatttatt ttgcggaagg
     9601 gaaattctta tacgaatggg gaagcataag tagcgatttg ttagagatca ttgataggat
     9661 tgaaattgtc gggaatattt atgagaatct agagttattt gaggtgatag aatgagtgat
     9721 ggttttgaac aagtatcaat aattaaaaat caagttcggg agattcttag aaaaaaaggc
     9781 tatttggtag atagttattt tgaaggagat tacgaaacat gggtaggtgt ctatgctcgt
     9841 ccagagaata aacctaccta tttagatcct acgacgtcag aagatgccta tttacaaaat
     9901 aggtatcgag tagatggttt taagcaagac tttgcggagt ggtttgaatg ggaaattgaa
     9961 aacggagaag taaaagaaga ataagagtac ttgttgtatc aaaataagcc agtagaacaa
    10021 ccaaaccagt agctagtaat gacaataata ctgaggaatc ggatcattat ggtatgattg
    10081 atattactct tccagaagat cttaagccag atcctgtaac aattggcaat agtgtcatga
    10141 tttttgattc attagatgaa gctatgtcat atggttggaa agaaattaag gatgaaggta
    10201 gtaattggta tggcatgaga tttggtgatg gtgaagttgc caaaaaagct ggtattccaa
    10261 ttgggaaatg gacagttgat ttccacgaag caggttcttt ttttgttctg taattatagt
    10321 gggagggtaa aatcattgat gaaatgtgtt cagataagag cgccagataa aaatagaaga
    10381 gacagttcac gtcgttatca cttttggtaa cggcgttttg ttttattata taaaattctc
    10441 aggaaaagga ggaattcttg tgtatgcaaa aacgaaaatg aattggttga aagcactcgc
    10501 attggtggct ttttctcaat tctgtttctt ggtgggaact gctcaagcac atgcagaaga
    10561 aacgacggct atacaaccta cgatggaagc cattgcccaa gtcgcttctg aagtgacaga
    10621 agtccaacca gcagaagccg gggtcccagc tggtgaagcc actgttgaac aggcccctgt
    10681 gacagaagcg ccaacggtca attccaaaac gactctcgat cagacgattg gggaaacaca
    10741 acacccaggg aatagtcctg gtacttcaca accagttgaa gaaactgcct caattacaac
    10801 ccctgcttca gaaacgtcca ctctagaagg agaaaaagct gccgaagcaa cgccttctgt
    10861 gcagccagag tcttctgaga aacaagcgga agaaaagaaa cgaatagaag aaacagtgga
    10921 acaaccagcg gctactgctg acaaagaagg aaaaaatgag gaaaacgttg aaaaaacaat
    10981 ctcttcaacc gcagctagtc aaaatcaacc aacgaaacca aacaaagaat ctacggctag
    11041 tattgaaaat caaaagaaaa aagagcagaa cattccaaaa acaggtacac ctacactgtt
    11101 agtaacagta ccaaccaatc gttcggttcg tgttcgccgt gcaaccaatg cagctgtttt
    11161 aggtggtcgg aaaattgatt ggggcgaaaa tattggtgtt acttctagtc cgaacaatct
    11221 aaacttcact ttaaattata tggttcgttt tgagattaat ggacaagtgg cgttttgtat
    11281 tgagcctggt gtctcagccg cagaaggtca agtttataca gctactgctt tgaaaaacta
    11341 tttgaaaaat tcaaatgtac ggaaaaaaac aagcttgatc tcttatttag gatatgtcac
    11401 aaataaagac aaatccgatg aacaatacat tgcggcacaa tttatgattt gggaagcttt
    11461 gggcacaaaa atcccaatca aaaatatccg tgttgattat gatcgtcgaa aagcagaaat
    11521 tcaacgtttg attaataact ttggtgaaaa agcaactttt aataatcaaa cacatacggt
    11581 aaaaattggc gaaacgttga aagtaaagaa taaaaatgat tttttgaaat atgtgaaaga
    11641 cattcaaaaa ccaaaaggtg tcaatgtatc tattcacggc aatgaagtat ggattacagt
    11701 cacaaaagag gcaccggata aagcaaaaat tcaattgaat aaaattaaag ttcaaggtgc
    11761 gccattagcc tatcgtcgtc caggagctca aacaattggt gtgttacatc caacagaacc
    11821 taatgcgtct gttattaatt tacaaattct aaaacaagga aacattcgtg tgtttaaaga
    11881 agatgcggaa actggtgtga aagcacaagg tgcagcttct ttagaaaatg cggtctatgg
    11941 tttgtatcaa gcagatggga aaaagattaa agaaattacc ttgaaaaaaa ttaacggcaa
    12001 agtacaggca gaaattaaag atcttgatcc tggcaaatat gttttaaaag aaatcaaagc
    12061 gccaaaaggt tatgtgcttt ctgatgaagc aattgaaatt gttgttgaac ctggaaaaac
    12121 tgtcaatgcg accgcaaaag atcaagtgat caaaggaacc attgatttag tgaaagttgc
    12181 taataaagat ttagtcgatt caacgaatcc agataacaaa ccaaaattag ctggcattga
    12241 aatcactttg acgtcaaaaa caactggcaa agtggtgaaa accgttgtga cagacaatga
    12301 tggttatgct acttttggta aaaataccgt tgtctttgat acgtacattc tttctgaaac
    12361 caaaggaaaa gaagggtata aactatttga accacttgaa gtcacaattt ctgaaaatgg
    12421 gcaaactttc cattatgttt tagaagataa agtaattgaa caacgtctta aagtggtgaa
    12481 agtggatcaa gaaacaggaa aagtgattcc acttgctaat acagaattca agatttttga
    12541 tactttagcc aatcgttatg tcacaatgga gattccaaat gatacagaaa caacggatat
    12601 ctttaaaacc aatgaaaaag gctttttcct aactaacggt acacttactt atggaaaaga
    12661 tcgctatcgt ttggaagaaa tcaaagcccc cgtaaattac gttttgaatc aaaacccatt
    12721 agtgttctcg attaccaatg atacagaacc gatcaaagtg atccattttg cgaaccgtca
    12781 agcacggaaa agtgtaaaat tattcaagta tgaagaatgg aacaagaaga aaacgccgct
    12841 cgcaggtgtg aactttacct tgtataacgg taaaaatcaa gcaattggtg aatataccac
    12901 tgatcaaaac ggggaaattt tagtgaaagg tctagttgcc gatgactatt acttcttgga
    12961 aaatgcccca ttagaaagtt tccaaccaaa tacagataaa caagcattca cggttgcatt
    13021 caaacaaaat gcgccagatg atggggaaac gttagtggtt tctgtacgta attacttgat
    13081 tccaccaaaa ctagaaacac aagcgtcaaa taaagccgat ggtgaaaaag tggttgatcc
    13141 attagaaacg atcgtcatta aagacagtgt ttcttatacc aacttgtttg tcggaaaaga
    13201 atacaccatt tcaggagtat tgatgaacaa ggcaacgaat gcgccaattt tacaagatgg
    13261 caaagaaatc acaagtacct tggcctttat tgcagacaaa gtcaatggca caaaagacct
    13321 tgaatttgtg gttaatgcta gtgttctgaa aggtcaaacg atcgttgttt ttgaaaaact
    13381 tttccgtgat ggcattgaag ttgcagcaca tgcggatatc aacgatgaag cccaaagtgt
    13441 gaaagtgaat aatccaaccg ttcataccac ggctgtcttt gaagacggat tgacagtcgc
    13501 agacccaaca ggattgttta ccttaaacga taccgttgaa attaccgaga ttcttccagg
    13561 aaaagcatac ttggtcaaag ggatcttaat ggataaagca accggtgaac ccttgcttgt
    13621 gcatggaaat acggtagaaa cgagtgttgg ctttattgcc aaagcagaaa atgaaacggt
    13681 gatcgtacca tttacctttg atttgacagg tataaatggt cgtgaaatcg tcgtgtttga
    13741 aagtcttttt agaaatgatg aagaactagc aaaccataaa gacatcaacg acaaagggca
    13801 aacggtacga gtaactaacc caagtgtacg tacaacggct acacataaac ccactggctt
    13861 gaaagaggtt aatccattaa cgaaagtcac cattacagac gctgtgaaat accacgattt
    13921 ggtcgttgga aaaacataca cagtcaacgg tgttctaatg gacaaagtaa cggaaaaacc
    13981 agtgctagta gatggaaaag aagtcacaag taccttaacg tttgtggcag aaacaacgga
    14041 tggtacagtc aatctagact ttattctaga cgcacgtgcg ttgcgaggca aagaagtcgt
    14101 ggtctttgaa gatctgttta gagaaggcgt attattggct tctcacgcag atattaccga
    14161 caatgaccaa accatcaaaa tcagtaatcc aaccattacg acgaaagcga cgaataaaaa
    14221 tggcggaaaa gagatcttgg ccttgccaaa tcaaactgtg cttgaatggg tcaaagtaga
    14281 cggtcttgcg attggtcaaa tctatcgctt aattgttcaa ggctacttaa caccagacgg
    14341 cacaccattt gaaggcactc atgcggaaaa agtctttgcg gccgataaag aaatgatgga
    14401 attcttattt gaattcttag tggacggtcg caatcttgca ggaaaaggct tgagttttgc
    14461 ggaatggctc caagcgaaaa cggaagatga gaaagaaacg tttgaagaag tggcgaaaca
    14521 caatgtagat ctaacaaaca aagatcaaac ggttcaaatt gtggaagcgc caaaaccacc
    14581 gcaaccagtg aaaccagaac aacgggttaa accagctact gttgtaccaa tgttgccaaa
    14641 aacaggtgaa caaacaagta gtatacttat ggttcttgga ttagtagtat tagtcattgc
    14701 tttaggtata acactttata aagtacgtaa agccaaagat tagtcatgag gaacaagcgg
    14761 ttaggatttt taaccgcttg tttgattgta atgtagaaat atttagaatt tattttggtt
    14821 tttaagagga ggaaaaactt tatggcagaa aaagcattgc gtatgaagaa agagttttta
    14881 ggtcaagtat ttttattggg ttcacgggaa cctaatccac caaaggaaat taatggtgtg
    14941 tctgggcgat atgttcaatt acaagcagag tgttcgcgag aagaacagcc tgcttttttt
    15001 gtttatctca cagctgaaga ggataaaaga ttacgattac cagaaaaaga ggtgcgtatt
    15061 tcatttgaag aagcttattt tgtttttcgt aaagaacgag tgaatagtcg tgaaacaaat
    15121 gatatgggac aaacagagcg tcgaacaact ggctatcaag atgttggtgt ctttttaact
    15181 ggtgtaaaaa ttggggaggt tttgtaagat gggattaaaa tttccaaata attatgacat
    15241 tacagaagca ttagatgaaa cttgtttagg agccgtacgc tttggcgcat tagaagagtc
    15301 tgatcgtgta tttgataatg aaacaggcga ttatacggga gaaattcgat cacagcgagc
    15361 acaattagtt tttggaaatc cagatgaacc aaagattgca cgacgagtta aatttccagc
    15421 agatgtagat ttgactggtt ttaaacgacg tgatcctgtg aaattaattg atccagaata
    15481 tcatgtggta tatattccag cacaagatgg caatcgtgaa tattcagatt tagtgattta
    15541 tgcgaaagga ttagagaaag tagaagctcc aacaaataat cctaatatga aacaatcaca
    15601 agaaaaaggt ttagatcaaa aacaaccaag tgttgattct aaggaaaaag agaaaaaata
    15661 aggttttttt gaaagaatag gaaggagtct tcctattctt tttatctaag atagaaggaa
    15721 ggggagattt tgaattcaga taaaaccaat caaatacttt gagagattgt gcttgttatc
    15781 aaaatatcca acaattgatt gagtattttg gagacaatcc aacgattttt gtacaaacaa
    15841 atgcatatat ttgtgcggtt attgggcagt gtatgttttt acgtgatctt gttcgttttt
    15901 cattaccgta ccagtggaaa caagatcatc aggaagtgat ttggattcgt atagtacgaa
    15961 tatgtaatat gtgtgcacac ccttataaaa atcgattaaa tctttttcga ttatgttttt
    16021 ttactaaatg tgatattcct gtattagttg ctttttgtca aaagttttta gcacaggaaa
    16081 agtaaaaaca gaaaggagga atgtgacaat tgaaagctca ttttttcaag tatcgtggtc
    16141 ggcgaattcg ctatttcaat cgttatttat ggttttgcta ttatggtttc tttttgtttc
    16201 catttttgat tctgttaggt tggtttagtt ggaaagtatt ttatccacgt ttaagtacgt
    16261 tttcgaattg gcaaatgtat cttattcctg gaataagtat tgtgttgtat ttgttgctgg
    16321 ttagtgggtt aattttaggg ttgatgcatt ggtcgcgact aaaagaagga tattttgcct
    16381 ctgtttattg gcgtcagtta cttgttcgaa tgttaattga caatcggttt gtttatacaa
    16441 agaaacaagt tagtgagaaa caaacaaggg aaaaaatgag attaccaaaa gtttattttt
    16501 atcaaaagaa acaagaatta gtggtttctt ttcctttaga tggtgggcgt tttcacgaac
    16561 gatttttaaa aatgggtcat ttgttatctg aagtattttt gcgtgattta attcgtgaag
    16621 aacgagaaaa agcccgaatc aattatgtat tcctttcaga ttctggtcgg attcctattg
    16681 atcaatgtat agcagaaaat ggacgtattc atttaatgaa aactttggat tgggtatatg
    16741 atcaatcacc aaatatgttg attgctggtg caatcggtgg tgggaaaacc tatttattgt
    16801 attctttaat tcaagcttgt ttaagtgttg ggacagtaga tatttgcgat ggaaaagcgg
    16861 cagatttagc agcattaggt gacgtcgatg tattcaaagg acatgtgttt tacaaaataa
    16921 atgaagaaat gattatttgt ttacgaaatg cattaaaaga aatgaatcaa cggtatgcct
    16981 atatgaaaac catgaaagat cctaaatatg aaccagggaa aaattatgct tattatgggt
    17041 taccaccgca ttttatcttt tttgatgaat gggcagcttt ttatggttcg ttggatatgg
    17101 aagcgagaaa tagagtcgat aaattgattc agccattagt attaaagggt cgtcaagcag
    17161 ggatgtacat tattttagca atgcaacgac cagatgcaga atattttcca agtggtgttc
    17221 gggataattt aacattccgt atttctgttg gtcgattgtc tcccattggt tatttaatga
    17281 cttttggaga agattttaaa aataagccat tttacaacaa agatcaacct ggtcgtggat
    17341 atgcttttcc aggatttgat gtacctgtag agttttttgc gccatttgtt tacaaagatt
    17401 atcactttat caaagaattt gagaaattta ctgaaatgat tgtctatgat tttacagtaa
    17461 aagaggatag tcaagatcaa acgatgggag atgataatca gttgttacaa gaacaactag
    17521 agcgccaaat gtatttagca aaagatccaa aaggaacaag tttttctgat gcgcatattt
    17581 tgtaatcaac taaggaggtg aagttaaatg gcagagagtt gttattatag agtagctaca
    17641 gcaatacgaa tgattaatcc ttctctttct tccagaacgt tttatgattg gttgaatcgt
    17701 attgaacaag taactgatta ccgctttttg cgcaaagaac gtgtttttac aggaaaagtt
    17761 attaatcaag tattgctaac gaaaaaagat atagaacgat tgactcgatt gtatcattat
    17821 cgagtagatt tagaagagga tttaactttg tcaatttatc gcgttttttc tcctgaaaaa
    17881 tatagtgaaa taacaaaatt agatcattta attttgtagt tattatctat cgggagggct
    17941 ctagacgtag tctggtagcc gcactctgcc tggcggtgcg cattaaggct gcgaagcatg
    18001 ggcctataat gcgcactcgc tagcagcgtg tgcagctcac aaagaggtcg tgcgcttgcg
    18061 cacggcctct ttgtgtaaag ccccccgata tataatatgg ggggcagaaa taacataaca
    18121 catagaaaaa agttttataa acccttttgt atcaagtgat tatctaaggt cagcagttgt
    18181 actacttttc ccaaaaaacg agggggacta gttgatatat cggtgtttct aaagggttaa
    18241 aaaaaatcga ttttgttatt ttataaaagg agcgagaaaa tgacaccgat tgagctaaaa
    18301 agctacagaa agaagttaag tgcaattcat aatataagtg gagataaaaa gccgctatca
    18361 caagcaagat ttgcgcaatt gatgggctgc tcaaaagatt acattagaaa gatggaacaa
    18421 gggaaaagaa aaatcccaga ggattttgaa caacgaatca aggaacgctt tcgattgtat
    18481 atgaaaagcg gaatagatct tcgagcaatg attgattatt tgcgtctttc cttttttggg
    18541 acagactaca aaacagtgat tcaacgtgta ttacgaatgg atctgacaga atttgaaaca
    18601 cgtccaacgg gcttgtatct ttttgactgt ctttctgttc gtgggaatat ctgggtgttc
    18661 tggcattcaa aacgaactgt aacagaaaat gtacttgtgc aattttcagg acaaggttgc
    18721 cgggaatatg aggaagtcat gcgtgaacaa caaacagatt ggcgaacatg gctatattcc
    18781 atttggaaaa atcaaggaaa gttggaagct ggttatacac gtgtgcaatg tagccgattc
    18841 gatattgcgt tagatgaact ttggggattg gaaacggaag aacatttcga tctctttgaa
    18901 ttactagaaa aacaaaaagc aggtttatta gaaatggact ttaagagatt ccagaatatt
    18961 ggcggttgtt ttttagatga tggctatttt cgaagtgaag gattatcttt gtattttgga
    19021 tcgcgaaaga gtcctctctt ttttaatttt tatgagaaac gttttgaaat tgcgaaccga
    19081 gaaaaaattt cagaattaga agcattaacg aaatatggta tttataatcg atatgagtta
    19141 cggttagcca atgagaaagc aacacaagca gtagaagcat ttattttagg tgattcaccg
    19201 aaacgtttag gagagattgg cgttggttta attaatgcaa aacttattgt ttgggataga
    19261 ctggaaagtg atgcacggat tgttgataaa cgatgggcaa aattatttcg ttcgatggaa
    19321 gaattggatt ttacagtaaa gccagaaaaa ttctcattag atcgttcaga acgctggttt
    19381 ttgaaacaag tagctccgac attaaagttg ttacaagaag cagatcgtat tttaggaaga
    19441 gactttgtac aagaagggat tgataatgcc gaactaaatg acgagcaaca aaactatcta
    19501 gaatggatgg gaacgtcagg agggaaaatt gcatgaatgc atatacgaca acagaatggc
    19561 tgcgcctgtt ggatttaaag gctgcaatcg aagcattaaa tgaaaagatg gttgatttgt
    19621 cttattttcg ttttcgagtt ccttatattg aacaagcggt aaaagctgga cgttatcagg
    19681 aaaaagagaa ctggcaagaa attgcacgct tattagaagt tcgtaaggga tacgaacaag
    19741 aactcgaaga gttggaattt agccgaagga aaggaaggct agaattcatt cgcttttatc
    19801 gattttcatt gccaatacct gcgattttag cggttaaaaa aggctgcgat aaaatgaaga
    19861 tatatgaaaa ctgtgtggca gcacttagca gcgaaaaacc tcttatggaa gaaatttcat
    19921 tggtgactgt cacttgggaa atgagtcgcc agccaacaga agaacagaca tatctttctt
    19981 tagaagagat tgagatagaa ttagaggaaa ttggtcgcta tgccacttgt tctacctatt
    20041 gtggatcggt cattagtatt gctggtgtga ttgtctaaat gaaaaaaatt atgatttatg
    20101 ttggtgcgta ttggtcacgt gatccaacag tattagaaaa tccagaggcg gtacactatt
    20161 gcttgcgcca attattctat ctgtataaag aacgattaga atcgttgatt cgtcagttac
    20221 catatactga cagacgttta gatgaacttc ttctaaggta tccagcaatg tataaacgac
    20281 gaaaaaatag attgttacca gaagagtatc ccattgaaaa acatgaactt gaagggcgat
    20341 ttgtggctta tttctatgat gatgttcgta tgcgcttagt agaacaacga atggaaatag
    20401 aaaatgatcg ctactatttt atcaattatt gtaagaagcg taagtatcaa gtaactgatg
    20461 atttttatga ttgtatttta caagatgggg aagtgattct atccaaaatt gctccaagtt
    20521 ttcgtgaact tgtgccgatt gattttttga aaaaatgtca tttgcggatt cttccgtaaa
    20581 aaagagctat aaaaggaaag aaaagtagaa gccaaatcgg tttctgcttt tttatttttt
    20641 ttaggaagga gaagatggta tgtcactgga atttttagga gagccaattg aaaaggaaga
    20701 gttcgtttcg cattatatgg atttgtttat tcaggtgata aaaaactttg tgcgattgat
    20761 gaattttatc cacgcgaaca agactatcta gccgctgaac gaataaaaat cagtttatta
    20821 tgggaaaaat tcacagatga aaataaccga ccgccagatt atcgctattt atcagattgt
    20881 ttaacgaata atgtgctttc taaagaatac ctatttcaca aacgtgagaa aaatcaaatg
    20941 tcggttgaac atttttgtga ttgttatatt gcattattgc gtagtcaaca gttattaaca
    21001 gtagaacgat atggtgcaga gtttggttat gtcatgcaag ctgagcgtca tagagctaca
    21061 agatgctatg aaaaagaaga tcaagtgatt gatggctatg aagaattatg tattcgaaac
    21121 aatcgctatt tgcaacaacg tttatatgaa caattgatga tggatttttc aaatctctat
    21181 agagggtatg aatgttcgat gattcaagaa aggtagaaga aaagggggaa tttttatgtg
    21241 gcgattatta tttttggtgg tatgtggtat ggcattgttc tatgtgtatc gtgtctatct
    21301 ctatccacga atggaacgat ggacaagttg gtatagagat cctttaaaac aacgaaaagg
    21361 attgaagcgg tatcgttatg aattacttgt tgaattttcg gatgggcgac aagcacagta
    21421 ttattttttt gcaaactatc caaaatacaa caccagtgaa ctattgattc aattattcaa
    21481 aaataatcca tatgttgggt tagaagaaaa cggacgggag ttcttttatc gttcagataa
    21541 tattagtgcg gcgtctatga acaaaacacg aattgcataa aaaattggag gaataatcat
    21601 gaaaaaaaca acattagcag tcgtaacaac actaggaatt tcattatttt cgtttcaatt
    21661 agggagttca ttggtttatg cagatgaaac tgtagaaaca cctaagtcag aaattgtaga
    21721 aacattgcca ggggaaggat taccagatgt tgagacagag ctaaatggag gaacaacgga
    21781 aaaaccagca gaacctgaac aattgcagtc agagaaacct gaaaaagagc cagtgacacc
    21841 agaaccagag tttcctgaat caactttacc gactgattcg atcgaagaag aaggtgtgca
    21901 tagttcaaca acaacggatt caagtatagt cacaactccg gaacaatcag gaacaaccag
    21961 ttcttctgaa aagaatactg attcgtcaag tacggaacag ccaacgatgt cgtctaccag
    22021 tgaagaacaa aaagaagaac cgaaacaacc tgttgaaaaa tcagaagcaa ttccaaaaga
    22081 aaataaacct attgttccag caaaagaagt cacagtatcg gtaaccccaa gcggtgaaat
    22141 tacgacgaat acttctcaag gaatgagtgt accgattgtc actagtaatg tagaggaatt
    22201 gacccatatt ccaacaccaa ccaccccatt aaaagtagca ggtgatcaaa caattgttgg
    22261 tgtaaaagat ggtattccat taatccaaga tagtgaaggg aatttggtaa aggatttgtc
    22321 gattcctgta aagaaacttc caagtggcaa tattgaaatc aaaacagcag atggaaaaac
    22381 aaaagtctta ccaaagactg gtgaagaaat acatgttgca ctttctgttc taggaggagt
    22441 gttgacaagt attgctggat ttgttggata taaaagaaag agaaaaggaa tagaaaatga
    22501 gggtaattga atggactatt tttcagaaaa acaagcttat cgttgtcaat cgtacgtact
    22561 agtaaaaaca tactagaagg agtagcttat gaaaaaacga caactacggc ggaaagaaaa
    22621 agtttataca gagaaagtac cgaagcaaaa gaaaatccat tcaaaaaaag cagtgaaatg
    22681 gatttggtgt ttggttttat ttctttgttt ttcagggggc ttggcatttt ttcaatccat
    22741 gaaaacgaag gttcaagtgt cgtttttgaa agatgaaatc caacaattac aaaagaaaga
    22801 agtacaaagt cggtcagaat taacgtacac acctgaaatt gaatccttta tgaatcgttt
    22861 tgttgctttg tatatgaata cattcgataa tcatgaacaa gaacaacaac gtcaagaaac
    22921 attaaaaaaa gagttttatg cacaaaactt tattcaagaa ccaggaagtg tttcaggtgt
    22981 tcgaaaatta actgctgctt cgtttattgg attaaaagaa attgaaacca ttccaactgc
    23041 tagttataaa gtaacttatg atattcgctc gatgaatcca gagagtaaga aagaaacaag
    23101 tgtgacaatc acacaagtac tcaatcttcc cttttctatt tcaaacaagg gctgtcgtat
    23161 tattgcttat ccttatttta cggcactacc aaagaataca gaaaaacaac gaatgattac
    23221 ttatgatgac acattatacg aaacggttga tgcgtcggaa gcgaaagatg ttgaaggatt
    23281 tgtggaagat ttcttagata agtatgcgaa aaacaatgca aaagatatgt catacatgat
    23341 gaaagaacct gaaggactca atcaaacagc taaagttgat caagtagaga tgaaagtatt
    23401 gcgttataaa gaaacgaaaa aaccttttat tgtaaaagca acggtcgtct ttcgagatgg
    23461 tgatacgtca cttttacaaa aagaacaaat gacgattgtc attacgaaaa aaacaaatca
    23521 gtattatgtt gaaaaattaa cacataattg gttggatagg aaaggagaag aacggaaatg
    23581 acactgaagg gaattgtaga ttttatatta acgcaaggtg gctatttttt aataatggtg
    23641 gttgtttatc tgggattgcg aaattttatg aaatcaaaag taggaaatgc attaatatct
    23701 atcgcagcag gtgccgcagt ttatttcttc ttaaatgatc caacacgtgt atttgatgcg
    23761 tttggtaaaa tcattgaaaa attatttatg gattaggagg atagtgaagt ggattgggaa
    23821 ccttatgatt attcgattgt ttatgcggaa ccttatcgcg tacaacagat tacaagcaat
    23881 tggcgtcttc catttccagt tgcattag
//
LOCUS       DAEGIQ010000037        23640 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000037 DAEGIQ010000000
VERSION     DAEGIQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.037"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            299..1960
                     /locus_tag="KMT45_001314"
     CDS             299..1960
                     /locus_tag="KMT45_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE7004784.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="KMT45_001315"
     CDS             complement(2030..2377)
                     /locus_tag="KMT45_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBE7004785.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="KMT45_001316"
     CDS             2461..3402
                     /locus_tag="KMT45_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004786.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="KMT45_001317"
     CDS             3403..4446
                     /locus_tag="KMT45_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004787.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="KMT45_001318"
     CDS             4452..5522
                     /locus_tag="KMT45_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004788.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="KMT45_001319"
     CDS             5512..6465
                     /locus_tag="KMT45_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7004789.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="KMT45_001320"
     CDS             6477..7487
                     /locus_tag="KMT45_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7004790.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="KMT45_001321"
     CDS             complement(7772..8593)
                     /locus_tag="KMT45_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBE7004791.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="KMT45_001322"
     CDS             complement(8690..8968)
                     /locus_tag="KMT45_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004792.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="KMT45_001323"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="KMT45_001323"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBE7004793.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="KMT45_001324"
     CDS             complement(11724..12311)
                     /locus_tag="KMT45_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004794.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="KMT45_001325"
     CDS             12486..13808
                     /locus_tag="KMT45_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7004795.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="KMT45_001326"
     CDS             13963..14739
                     /locus_tag="KMT45_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBE7004796.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="KMT45_001327"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="KMT45_001327"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBE7004797.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="KMT45_001328"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="KMT45_001328"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBE7004798.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="KMT45_001329"
     CDS             16629..17258
                     /locus_tag="KMT45_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004799.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="KMT45_001330"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="KMT45_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBE7004800.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="KMT45_001331"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="KMT45_001331"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBE7004801.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="KMT45_001332"
     CDS             19445..19981
                     /locus_tag="KMT45_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBE7004802.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="KMT45_001333"
     CDS             complement(20021..21784)
                     /locus_tag="KMT45_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004803.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="KMT45_001334"
     CDS             complement(21787..23502)
                     /locus_tag="KMT45_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004804.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7620 a         4321 c         4799 g         6900 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
//
LOCUS       DAEGIQ010000038        23128 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000038 DAEGIQ010000000
VERSION     DAEGIQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.038"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="KMT45_001335"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="KMT45_001335"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBE7004805.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="KMT45_001336"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="KMT45_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBE7004806.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="KMT45_001337"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="KMT45_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBE7004807.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="KMT45_001338"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="KMT45_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBE7004808.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="KMT45_001339"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="KMT45_001339"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBE7004809.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="KMT45_001340"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="KMT45_001340"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBE7004810.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="KMT45_001341"
     CDS             complement(8053..8523)
                     /locus_tag="KMT45_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBE7004811.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="KMT45_001342"
     CDS             complement(8569..8994)
                     /locus_tag="KMT45_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBE7004812.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="KMT45_001343"
     CDS             complement(9061..9330)
                     /locus_tag="KMT45_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBE7004813.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="KMT45_001344"
     CDS             complement(9341..10936)
                     /locus_tag="KMT45_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBE7004814.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="KMT45_001345"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="KMT45_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBE7004815.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="KMT45_001346"
     CDS             complement(14489..15049)
                     /locus_tag="KMT45_001346"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBE7004816.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="KMT45_001347"
     CDS             15402..16376
                     /locus_tag="KMT45_001347"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBE7004817.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="KMT45_001348"
     CDS             16546..17946
                     /locus_tag="KMT45_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBE7004818.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="KMT45_001349"
     CDS             complement(18024..18746)
                     /locus_tag="KMT45_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7004819.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="KMT45_001350"
     CDS             complement(18825..19331)
                     /locus_tag="KMT45_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBE7004820.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="KMT45_001351"
     CDS             19454..21253
                     /locus_tag="KMT45_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBE7004821.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="KMT45_001352"
     CDS             complement(21321..22895)
                     /locus_tag="KMT45_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBE7004822.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6331 a         5021 c         4225 g         7551 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct ccttttagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcg
//
LOCUS       DAEGIQ010000039        22648 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000039 DAEGIQ010000000
VERSION     DAEGIQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.039"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            127..3654
                     /locus_tag="KMT45_001353"
     CDS             127..3654
                     /locus_tag="KMT45_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBE7004823.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="KMT45_001354"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="KMT45_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBE7004824.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="KMT45_001355"
     CDS             7442..7783
                     /locus_tag="KMT45_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7004825.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="KMT45_001356"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="KMT45_001356"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBE7004826.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="KMT45_001357"
     CDS             9140..11560
                     /locus_tag="KMT45_001357"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBE7004827.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="KMT45_001358"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="KMT45_001358"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBE7004828.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="KMT45_001359"
     CDS             12994..13731
                     /locus_tag="KMT45_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBE7004829.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="KMT45_001360"
     CDS             13745..14560
                     /locus_tag="KMT45_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBE7004830.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="KMT45_001361"
     CDS             14573..15220
                     /locus_tag="KMT45_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBE7004831.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="KMT45_001362"
     CDS             15236..15892
                     /locus_tag="KMT45_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBE7004832.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="KMT45_001363"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="KMT45_001363"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBE7004833.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="KMT45_001364"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="KMT45_001364"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBE7004834.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="KMT45_001365"
     CDS             18234..18752
                     /locus_tag="KMT45_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBE7004835.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARVATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="KMT45_001366"
     CDS             18859..19359
                     /locus_tag="KMT45_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBE7004836.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="KMT45_001367"
     CDS             complement(19639..20559)
                     /locus_tag="KMT45_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBE7004837.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="KMT45_001368"
     CDS             20724..21737
                     /locus_tag="KMT45_001368"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBE7004838.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="KMT45_001369"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="KMT45_001369"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBE7004839.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7542 a         3951 c         4824 g         6331 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat cagggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta cctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgtgcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 tcaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agttgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaa
//
LOCUS       DAEGIQ010000040        21940 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000040 DAEGIQ010000000
VERSION     DAEGIQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.040"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(124..669)
                     /locus_tag="KMT45_001370"
     CDS             complement(124..669)
                     /locus_tag="KMT45_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBE7004840.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(681..1373)
                     /locus_tag="KMT45_001371"
     CDS             complement(681..1373)
                     /locus_tag="KMT45_001371"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBE7004841.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1392..1676)
                     /locus_tag="KMT45_001372"
     CDS             complement(1392..1676)
                     /locus_tag="KMT45_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004842.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1689..2252)
                     /locus_tag="KMT45_001373"
     CDS             complement(1689..2252)
                     /locus_tag="KMT45_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBE7004843.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2480..3142
                     /locus_tag="KMT45_001374"
     CDS             2480..3142
                     /locus_tag="KMT45_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBE7004844.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3266..4462
                     /locus_tag="KMT45_001375"
     CDS             3266..4462
                     /locus_tag="KMT45_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBE7004845.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4563..5939)
                     /locus_tag="KMT45_001376"
     CDS             complement(4563..5939)
                     /locus_tag="KMT45_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7004846.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVIILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5976..6842)
                     /locus_tag="KMT45_001377"
     CDS             complement(5976..6842)
                     /locus_tag="KMT45_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBE7004847.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6931..7956)
                     /locus_tag="KMT45_001378"
     CDS             complement(6931..7956)
                     /locus_tag="KMT45_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBE7004848.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7974..8648)
                     /locus_tag="KMT45_001379"
     CDS             complement(7974..8648)
                     /locus_tag="KMT45_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7004849.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLVRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8832..9725)
                     /locus_tag="KMT45_001380"
     CDS             complement(8832..9725)
                     /locus_tag="KMT45_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBE7004850.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9799..11496)
                     /locus_tag="KMT45_001381"
     CDS             complement(9799..11496)
                     /locus_tag="KMT45_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBE7004851.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11489..12802)
                     /locus_tag="KMT45_001382"
     CDS             complement(11489..12802)
                     /locus_tag="KMT45_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBE7004852.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12802..16839)
                     /locus_tag="KMT45_001383"
     CDS             complement(12802..16839)
                     /locus_tag="KMT45_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBE7004853.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16852..17310)
                     /locus_tag="KMT45_001384"
     CDS             complement(16852..17310)
                     /locus_tag="KMT45_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE7004854.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17607..18125)
                     /locus_tag="KMT45_001385"
     CDS             complement(17607..18125)
                     /locus_tag="KMT45_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBE7004855.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18127..18744)
                     /locus_tag="KMT45_001386"
     CDS             complement(18127..18744)
                     /locus_tag="KMT45_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBE7004856.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18741..19595)
                     /locus_tag="KMT45_001387"
     CDS             complement(18741..19595)
                     /locus_tag="KMT45_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7004857.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19592..20503)
                     /locus_tag="KMT45_001388"
     CDS             complement(19592..20503)
                     /locus_tag="KMT45_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7004858.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20544..21878)
                     /locus_tag="KMT45_001389"
     CDS             complement(20544..21878)
                     /locus_tag="KMT45_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE7004859.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
BASE COUNT         6379 a         4814 c         3784 g         6963 t
ORIGIN      
        1 taacggaaaa agaataagtt tttttaaaat aacgtataaa aaagagcata aaacaaagtc
       61 gtttgtttta tgctcttctt ttattatttt gtgcttttta ttttatcaaa tcactttgtt
      121 tttttataaa atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc attcatgtcc
      181 aacgggatcg aaacttgcca cggaatcttt gaaaatatgg agtttgtatc ctagattata
      241 cgcatcgact gctgtatgca acacacagat atctgtacat acgccggtca aataaatatc
      301 tgtgatctgg cgttctcgca aacggatgtc tagatctgtt ccactaaaag cggaataatg
      361 ccgtttgtct atccaataaa cattttcctc ttgctcatag tcttggtata aactttttaa
      421 agagccgtac aattcccgtc cacccgttcc tatcacatta tgcggaggaa ataagcggtt
      481 ctccggatga aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga caaaatcctt
      541 ttgatcaata aacttctttg tatgagcagt cagctttttt tcaattactt gtcctgctgt
      601 cccagtagtc agctttccat cctctgcaac aaaatcatat gtgtagtcaa tcgagatcaa
      661 tgcgttcatc ttagtcctcc ttatacgaga tgtttgacaa attcgatcac catttctgct
      721 gatcgtttgc ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga gtgatctgct
      781 gtgtcgctca ttgcacgtac gatcaaaaat gggatattga actgacgagc tgtttgtcca
      841 acagcggctc cttccatttc gcaagctaat gcttcaggga aattcgttaa gatttctttg
      901 attttatctg gcgagtcgac aaatgtatca cctgtaacga tcaatccttc ttttgcgttc
      961 aaattcgttg cttttgctgc tttaaccatt tcactgcgca aatatgtgct tgcttcataa
     1021 tatagaggca ttcctggtaa ttgtcctggg ttgtagccaa atcctgttgc atctgcatca
     1081 aaataagcta ctttgtctga tataacgatg tcacctactt gtaagccttc accgattccg
     1141 ccagctgaac ctgtattgat caccatgttc acgccgtatt gttggatcaa cagacttgtg
     1201 gtaatagatg ctaagacttt tcctataccg gaacgtacga cgatcacttc gtggttacct
     1261 aatgaacctg aaataaataa agcgcctgcg cgttcccatg ataatggttc gcttaggttc
     1321 tctctcaaaa tttttacttc ttcttccatt gcgccgataa taccgatttt catcttttta
     1381 attccttctt tctatataaa tgcgattgct aaaaaaacca cacataacat taggataaca
     1441 ataataagag atttcatcaa aaaagagttc catttggtcg tcttttcccg ttcgctgatc
     1501 cttgttttcg tgattggttt attctttttg ctttctttac gataaaagct ggcaatcttt
     1561 ttttcttctt gctgataagc tgtttctgct tttcgctgtt tttttaatga ctcgcttgct
     1621 tgtgcttgtt gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt ggccataagc
     1681 cgatccctct actttccttt ggtcaatagt tcccagtatt gaatcgcata aatcgtcttt
     1741 gcatcacaaa tcaactgatc tttcatcgct tgttgcgcct cttcaagagt tagatgatac
     1801 aactccagta cttcgtcttc atcttgtgct aaaggattct cgaccttctc taccccttgt
     1861 gcatgataaa tatgaaggac ttcatttgca aaacctggtg aaagatacat cgaagtaaga
     1921 tggctcagcg acttcgcacg atagccagtt tcttcttcta attctctagc tgctgtcatt
     1981 tcgggatttt gtccttctcc tggatcgatt tttccagctg gaatctccaa aatcactttt
     2041 tctaaaggtt ttcgaaattg tttgacaaga agcagacgat cttgttcgtc aaaagcaatg
     2101 atcccgacac cgccaggatg aaataccagt tctctcttcg aagttcctcc atctggcaaa
     2161 cgaacttcgt ctacagcaac atcaataatt tttccgctat agatttcttt gcgactgatt
     2221 gttttttctt caaattgctc attgttcagc atatcttctc ctgctttctt tgtaatcata
     2281 actgcttttt tttcattttt caatttttct caaaaactcc ttattagttt accatagata
     2341 aaccttagat tttctctaaa tatagaaaat ccttctttag tccgatactc tttctagtag
     2401 tccgtatgct aaaataagta tgtaatcgaa taacgaaaag aattcaatac tcaacgataa
     2461 atgacatgga gggtttatta tgataaaacg aaattggccg tggcttctag cattggtctt
     2521 gctatttctc tttgtccgaa acaatagttt ttcttttgtt ctaggacttt tattgatagg
     2581 tgtagtttta gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa aacaagaaga
     2641 aattcctgca ctgactgacg aacttgaaac tcactatgaa aaaagcggta tgacagcgaa
     2701 tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca atcaattgca
     2761 aatgaatatg cagcagacag ccaaacttaa agcaatcgat ctccgccatg atccagtcaa
     2821 agcagcgaaa gctttattca aagaattggt gaaagaacca aaccgactgc atgaagcaag
     2881 tcagtttctt tatacgcatc tgccgaatct agttgatttg acaaataagt ataatgagat
     2941 caatgaccat gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa gcgcgcagat
     3001 catcgaccag ctgtcagctt taatcgcaca agattatcaa aaattcgttg ctgaggatct
     3061 agatgatatc gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg ataataaata
     3121 tgaaccagaa caaaaggatt aactcttttg ttcataaaag aagactcgga acattcagat
     3181 gatgtctggc tgtttcgagt actttttcta aagtagagac attcatgtaa gaatcgagaa
     3241 cagattcaac aaggaggaat tttttatgga tcataaaccg actgaaacaa aagatgttac
     3301 accagttaac gatacacttg aagatttatt gaacaacccg ttttcaactc caacagatac
     3361 gctgaccact tcacaacaag cggagattga tcagctccaa gagcaacaga cagctgctcg
     3421 tttaattgac aaactaccgg cggaacgcca agaacaagct aaacagctag cagcaaaaat
     3481 cgatgctaat gatgcacagt ctgtcatcag ctacggatcg gctgcccaag caaaattaag
     3541 tgaattctca caatcgatgt tgaatcatgt gcaagcacaa gatattggtc ctgtaggaga
     3601 ttccttaact gaactgatgt atcgtctcca agaagccaat cctgacgaac tgcgtgccgg
     3661 agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct atgaagtgac
     3721 agcaaaatat caaaagattg gtgcacaaat tgacaagatt tcagtgaaat tagataaaga
     3781 aaaagacggt ctgctaaaag ataatctgat gttagaacag ctttaccaga aaaacaaaga
     3841 ctactttgat gcactgaata tttatatcgc tgctggagaa ttgaaaatgg aagaacttca
     3901 aacaacgatc attccagaag cgatgaaaag agcagaagaa actggcgatc agatggatgt
     3961 tcagattgcc aatgattaca cgcaattcct agatcgtctg gataaacgta cgcatgatct
     4021 tcgcttagct agacagatca cgatccagca agcaccacaa atccgtttga tccaaaacac
     4081 aaatcaggcc ttggctgaaa agatccaagc atctatcgct acagccattc cgctgtggaa
     4141 aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg
     4201 ccaagtctct gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc
     4261 agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac
     4321 cactcaaaat gatttgatcg aaacaatcca agagacattg cgtatccaaa aagaaggaaa
     4381 agaaaaacgt cgtcatgcag aagtagagct tggacacatg gaagaagatc tgaaacaaaa
     4441 actgttggat ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa
     4501 cagtttatta caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga
     4561 gtctatttct cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca
     4621 ataaccatta cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca
     4681 atacaagtca agatggagac gattggcaat agcggaacga atggcacttt gaattctcca
     4741 gcttttggtt tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa
     4801 taggcgagcg ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca
     4861 aagaatacag ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga
     4921 tcgatttctg tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga
     4981 gcaagtgaat acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg
     5041 atcaccgcgc ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg
     5101 actcctagtt cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg
     5161 acagtagcaa tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg
     5221 acttcctcgg ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct
     5281 gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta
     5341 acgaaaaata ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg
     5401 gcattattca aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt gacgaatatc
     5461 actacgagca ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt
     5521 aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat
     5581 ccggaagcaa cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc
     5641 caggcagcaa attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc
     5701 actcgcgaag caaattctgc gaaacttagt ccagacaaaa tgataacgat tgcagctaag
     5761 acaaatgaaa gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca
     5821 aatattcccg taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt
     5881 tcttttttca tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt
     5941 gaagttcctc cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa
     6001 aggataccac ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc
     6061 gtttttcttt cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt
     6121 gaaagtgaga agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt
     6181 cctaagcccc ataataatcc acagatcatg tttttccaca caaactgatc gactttaact
     6241 ttacggaaag caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt
     6301 gacatcgcat ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta
     6361 gagaagatca atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt
     6421 tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca
     6481 ataataccgt atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca
     6541 atcgtattca aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg
     6601 tacttcattc catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat
     6661 ccgatcaaga aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa
     6721 aacagaaatg caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta
     6781 ccacttacta aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc
     6841 ataaccaatc tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca
     6901 caagcacttt ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat
     6961 tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat
     7021 acctaatttt tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt
     7081 gctaagtcga ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc
     7141 tgggataccg atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat
     7201 gctcacaatg atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt
     7261 gaagataaaa gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc
     7321 gtagcgaata tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat
     7381 tccttttagt ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata
     7441 atacaaaact tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata
     7501 cttttgatcg tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg
     7561 cactttgatt tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat
     7621 tgctgattgc attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag
     7681 tttttcttct tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat
     7741 gtcggctaat tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat
     7801 cagaaagacg ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt
     7861 tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa
     7921 tgtccactga tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga
     7981 acgatatatc cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt
     8041 tttcggcgta accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca
     8101 tcccacagtg ctcgcaccaa tttttcccga cttaagatct ttccattgtg cttcatcaaa
     8161 ataaataata gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata
     8221 cttccgttgt caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct
     8281 gtgtagttgt aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg
     8341 aaatgttttg tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc
     8401 gtattcctgc tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac
     8461 caataatagc catccaaaac gggaagatta atatctagta atacaagctg tggttcttct
     8521 ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat
     8581 ttctcaagtt cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt
     8641 tttgccataa aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg
     8701 tgaaacaagt tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa
     8761 tatgcgattt aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa
     8821 taattggtct attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa
     8881 caccaataac cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga
     8941 taaagatggt atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact
     9001 cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt
     9061 gtgactagat cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca
     9121 attttctcaa cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca
     9181 tcttgctcca tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg
     9241 gtaatcacgg aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc
     9301 gctcctctat ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt
     9361 ttcggaatat agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg
     9421 acatttctaa ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat
     9481 gtatgagcaa gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct
     9541 tctttatcgc gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg
     9601 ctgagagcat ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact
     9661 gccataaata attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt
     9721 ttcattttct tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct
     9781 gcagcttatt tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta
     9841 cgctccaatc atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt
     9901 agcaggaagt gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc
     9961 tacgatctga atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc
    10021 ctcacctgtt ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc
    10081 ataatctaac ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg
    10141 tccgcttgtt gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc
    10201 ccctttataa aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc
    10261 tttaagcaat tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac
    10321 tttctctggc ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat
    10381 aaccgctgtg ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata
    10441 cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc
    10501 tactccattt tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat
    10561 ataatcttgt ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata
    10621 ataagttaga ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc
    10681 gttttcaagg acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt
    10741 ttttcctctt tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat
    10801 cgttacttgt ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc
    10861 gtttttcgga tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca
    10921 cacgccatcc accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat
    10981 tctggaaata tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga
    11041 aggtgcttct tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt
    11101 ttgtttgtca atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg
    11161 aatccattca atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt
    11221 atctcgaatc aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg
    11281 aatattttct tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac
    11341 agatgagaaa ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc
    11401 ttcggctttg actagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag
    11461 tacactcatt cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta
    11521 ttaataaaac agcacctaac aataaaagaa ttcctagtaa agaaatcaac gttcttgctt
    11581 cattcgtttg cgggaatacg gaccattttc ctgattgacc tggattgctt ggaccactcg
    11641 gctctttcgg tttgtccgtt gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg
    11701 attcataggc agagactgga actttcccgg attctaattc agccatctcc tgtccgttaa
    11761 ctactacata ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg atgacctcga
    11821 ttctcttact tgcttcatcc atctcgtaac cttccacacc ttttaattct tcaaaataat
    11881 aggtgccaga aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta tccgaaatga
    11941 atttgcttac atttttgtca ttcaaaggat cattgtctgc tggtttgatc cattgatttt
    12001 tcaagtcatt agcaggagcc atatctaaat aatatttccc cccttctatc atctgatata
    12061 gagcaaaaac agcaccttct aatggttttc ctttttcagt tgttcctctt tgacgtccat
    12121 acttgaagaa gtatggatct cttagataac cgacattttt aggatagata tgaatctctt
    12181 gcagttcctc ggttgattct gtcgggtgat aaattggcaa aatagcagca attgggatag
    12241 ctgtctgatc gatatcaaca tccaatccaa cttctggatc aatcgattct tcgaaaatca
    12301 aataaactgc atcccgatct tggtttttct taggtgcttt gatacgtgca atcccgtctc
    12361 ctaatccgcc aaccgtatct ttcccagttg ttaccgaagt ccctatttgg acaagccttt
    12421 cttctgtgat tagctgacgg atattgtttc tgttcgtttc agcgatttgc tggacgaatt
    12481 ctttaggagt catgttttca gcagcttttt tttcatgata ataatcggtc atgtcgtaaa
    12541 tggcaaatgt cgctccattt agtggcaccg tctgtgaaat gatatctgct gtttcccctg
    12601 cttcctcaga gatagccaag ccatcatttt catggtattc aaattgatcg ttataatcga
    12661 tatctcgaat catccgttta tgcaacacga attccacgac atcttcccct gattcttctg
    12721 ccgacatcgc atgtggaaga aggactgctg caagcggtag taaaacaagt aattgaagca
    12781 tcaaacacaa cagctttttc attaccttgc tccctccttt ttagattgtc tgacaaacaa
    12841 accataggct gctcctgcaa gcgccatgaa gaatgcagca agcaggatat agaataatcg
    12901 tccggatcct cctgtagcag gcaacggatt ttcaggatcg ttcccgacac taagctgtaa
    12961 tctgattcga tttgtctcca aaagttcata ttctaactct tgctgtagat caggcgtctc
    13021 tgcttcttcg tttggataat acgaaatctc aacttttagt gtacccgctt cttttcctgg
    13081 aactacccga tcattttcta tgtcaggttc ttctcgataa ctaatttcta aaataaagta
    13141 acctggaagt tttctatagc tgtcaggtga cttcacttcc ttgatccgat agaccccttc
    13201 caacaagtct gaagcattga atagggaaga atcgtttgga tctggtgcca agcctccctc
    13261 gatgatttca tactctcctg cactattttg tttctcaatt gaaaattctg cgccttcaag
    13321 acgtcggcta ttcatactgt caattttcaa aagttccaaa tcgattggcg ctagttcatc
    13381 ttctaaggtt acttgtagta caccatcgat gatttcatag ccttccggca agggagcagt
    13441 ttcgtcttcg atgaccgtat cttcaatgat aaacttacct tcttcattca gtgcaaactg
    13501 gaacacttga tcagatagcc gatacccatt tggtgcgaca gattccgaga cctcatattc
    13561 accaatagac agatgcagag gttggtcgta atttaaatcg acatactttt ctactcctac
    13621 ggcacgagaa tagaagtatc cgatcccagg caaacgtttc gattcttctg gttctaacgg
    13681 attaccagtg tatgctcgat acagcgggaa ggaagaatcc cccactttcc tgatatcgaa
    13741 acctgcacca tctaatgtat ggccttcatg gtctaatttt ctaactcgga tctcccgata
    13801 atcaaacgta ttgtaagcag tgaatgctac ccataaacga tcctcttcat ctgttgtata
    13861 cgtgagatat tgccaatttt cttcacctgt ttgcttgagt tggatagtcc ctatagcatt
    13921 atcgtaaagt cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt
    13981 ctggctctca atgattttgt attctgcata gttggtagtt tcttcactat ccttataaac
    14041 aaatgaatct ggctctccga ttggatgaat aactttctca ccatccacga ttacatattt
    14101 cccgtcttca tctactgaat agaacgtgag tctccctttc tcttcctcta cttcgttcac
    14161 accagaaagc cctttcgcta ctggatatcc gatttccagt ccgttaccat ctgtttcata
    14221 aagttcaaac tctgcgccat tgattggcat attactgcct tcaattagtt tattgatatt
    14281 catgtggatt ggcttgaatt catttcgcat ttccagtttg acatacaact catcatactc
    14341 tgtgccttct atgttaggct cttgtacaac agaaagcttg tgatattctc cttcataata
    14401 ggaattcgga tctttacctt ccggtccgcc gactctcgaa ccagatgtat attcatctac
    14461 aagctcgtag tgacctaaca tctcatcttc gttggctgca tattttttga tttggaattg
    14521 atacgtctca tctactaact gataacgtgg tggtccagcg atctctctga tccaaaatat
    14581 gtcgctgact gctccagttt catctcctaa aatcggcatc ttcgtttcaa agatcgctat
    14641 tccgtccgtg gtagttttac ttgtttcgat tggttcggct tccccactct ctggattttg
    14701 gtcatataat gcaaattcga ctccagccaa cggctcgcca ttttgaccct tcttgctgac
    14761 ttggaaatta cggcctttat gttcgtaacg aatggaagaa gatgggaaat acatccgtcc
    14821 agtagtgata tcattctcat aatcctccag gttctgaata taggccatat tgttcactgt
    14881 gcaaaaattt ccgtcttgag ctcccgcatc aagttttact tggtacttga tcctgaccat
    14941 ttccccttca cctaaagaaa tcggagaaaa tttataagct tggttttccc gtccgacata
    15001 cgttgtcgga gtcgccacta cttctggtgg ggcttgtcca tgacgttctt catcccatat
    15061 ttctggctct tggtacttca tgccgttttc tcctggatct agatggtaaa actgaatatc
    15121 tgccgatctg ccagctccaa atggtgagac attaaaacca gtttcatcat aaagtgtggc
    15181 ttgctggatc gttctcttga aggtagaaga gatatcctcc aaggctgtaa caatgtcatt
    15241 cttgttgttc gcattgtaat atcgcggtgt tacgccatct tcttctaaat cagcaatatt
    15301 tttcaataca tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt ttccatcgat
    15361 tcctaaacca atggagaaga tacttggcgg gttctcttga tacttgtttt tcagccaatg
    15421 atgataagca actgtgttga tcgcagatga cttttcttgc gaagtaggac tgccattcgt
    15481 attgttatta gaagaaccat caccaaccca gtgagtcgct ttattatctc cttcttttac
    15541 ctctccataa cgatagtgta catttacttc atcttctgga cggttaaagt cagatgcata
    15601 ggtaactgga tgagtaaacc ctgcattaga agtattccca gaaaactcct caaaatttct
    15661 gaacaaaacg tcattatctt cagcaatctt atcttccatc acagtccagt tgctccaagc
    15721 tccattagtg ctcgtacgat aacttgactg aatcggtgca taactgaagg tcggtgtccc
    15781 atctcccaca acgattagga ttttttctgg atttctattt tctccgccat tgtctttata
    15841 caatgtttca tagccatttt taagccctag tgtaagcgga gttcctccga ttggtgtacg
    15901 atttaatgct gtattctcac ttcccgtcaa acgattgata tcatttgtca atggaaagat
    15961 gtccgaactg atttgctcat ggttattgat agctgtacta ttatggtaaa aattcaccat
    16021 accgattcga atatccagac tcgggtcaga gagcagatct ttactcattt cgtttactgc
    16081 ctcaatcaac gcagcatttt tagtttgact attggtccca gcggttaatt cactcatact
    16141 cgctgactta tctagaacaa aaacgatatc gattggactg atttcctttt tctcgccccc
    16201 gatgacatct agatagacat tgtaaagggt tgttgcatct tcaggatgta gatcagatgc
    16261 tgtgctgctt ggcagaacag gttctaccca ttttttgatg tacgccccgt tataatccaa
    16321 gtaactgttt tcaaaattcc gttcatttcc ccaaagctga acggtttgag ctggaacgtt
    16381 tctatcttct tcgcctgcag ttgatccatt attagtagca tagtcgtaat ttcgccagtt
    16441 cgctcccccg tttgtataat cactatcatc gatcaagttc tctccgtcat atggcgagcc
    16501 tttgtctcct tgatagcgtt cattgctttg tgtccaattt cctaacactt ttgtatagtc
    16561 attcgttgta aacttcgggt agcgatgctg ttcgttttca acttctccgc taggatagat
    16621 atattcatag gggtcttgcg tagctgttgg cccaagacgc tccaaaatac cgttgaataa
    16681 ctggcgttga atgcgctgga gtgcttgacc gctgggtacg attggttcgt ccagcttttc
    16741 catctcattt tcttcgctac gttgaatttc agcggaatct tctgtatcag tttcattttc
    16801 ttgtaattct tggttatcta ttctttcatc aaccaccact tgaagaggta gttacccttc
    16861 gttcacatcg gggatctttt ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga
    16921 ataaactttt ccgacatacc attcttgacc atcgatcgtc tcgactttta ggtcttctcc
    16981 atcttcatca taggaggata gacctgttcc acgaacgttt tttacggtac caatcccggc
    17041 ggctgccgca tcgattgcta aacgtattct accttcattg gcactttcct ttttttcaaa
    17101 agccagatgc cattcaaggg tttcttcttt ttcaatatac ttcccttcaa tttttgcata
    17161 gtcctcatct aagaatacct catcctcctt tgtttctgtt gactcagcag ctacatccag
    17221 ggtcgtcata tgatagactg actggaaata tggtccaaat agcaaactga cagttactaa
    17281 ctgcaaaagc ttcaatttta accctttcac atccttatac tcctttcaaa aatatttgtc
    17341 ttctttcaca acaatcaaca aaacaatgcc catctaatta taatttaatt taattttaat
    17401 tttaaatcaa atgatatcct ttttagttaa aaacaccaat tatacaacat ttattattaa
    17461 aattaaatta taaaatttta taacgttctc atttaacaaa acttaagtta ttaagacaaa
    17521 tgaataaaca actacgcttt cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc
    17581 actcatgctt tattcgtaca attaattcag ataacggcag cgcataggat gccaataaca
    17641 cttcctaaaa tagctcctcc taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc
    17701 gaataagcga tcaaacaagc aaataaaaag gctaatattc ctgcaactgg acttaattcg
    17761 aacaacgcag ctactgataa aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc
    17821 ttcggtttac ttgtgtaaag gacaagccga cgattttttt cataaggtct tttccgtctt
    17881 gtcgtttttt tgataaaaag caaaccaaca aaaaaataga tcaaactgcc gataagaatg
    17941 gctagaccat attctctagt atcaggtatc aagactaaaa cagcagcaag gagcaaccaa
    18001 ataaaaccac cattagagat attactgata aatttcaaaa aattgatttt tcgactatcc
    18061 gttttccgtt gcttggcgaa cttgttgata atactatcat cgattttagc aatcaaacgc
    18121 aacatgttat tcctcctttt taagaacctt ttcttgcgta cgatggataa aaagcaggac
    18181 ttgaaaataa aacaagaaac tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca
    18241 gaatgcgaca agaaggatca acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt
    18301 aaaaaacaag aaaaatgcag taatccaaga agcttctttt tgttccagtt gaagcaagat
    18361 tttgtaacta aagagaagcg cgcttaatag aagcaaaaac aacgtagtat acatcaatgt
    18421 atagccaatt cccccacccc aatagcggaa aatctgataa tcgatcaata gaagaagaaa
    18481 ataaacactt gctaaactat cttgctttcc ataaagagag aagttctcac gaaacttttt
    18541 ccagaatagt gaaccgagct ttttctcttc attttttttg taatgataaa tcatatacag
    18601 aactgttcga cttgcagttc ctaatgtgaa aacaggcaag acgcacagga accatagtag
    18661 ctgtaaatgg ataagacgtg gaatccattc aatgatttct aagaaataat cattggcttt
    18721 tttaccaatc gatcgattca tcatcctttt gaacctcctg tcaaatccat cccttcaatg
    18781 aaataacgtt gtgcaaaaaa gaacagcaaa atcgtcggga tcaagacaag tgttgctcct
    18841 gccatcagat aattccattg tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt
    18901 aacgtgtatt tttcttgatc ttggatatat aacagaggac ctaagaaatc attccaagct
    18961 gcgttgaagg aattgatcgc aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa
    19021 tgactccaaa tatacaaatg attcgctccg tcgatttcag cagcttcgat caattcatta
    19081 ttgatcgata agtaaaattg acgcatcaga aatatattga acgcattgcc gaagaacgat
    19141 ggaacgatca ggggcaaata agtacctacc cagccgatct tggagaataa tacatattga
    19201 gggatcatcg ttacaaaccc cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc
    19261 tttttcccag gaaaatcaag cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca
    19321 ttaccgatca ctactagcac agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga
    19381 aaggcggcaa tcgtatccag atagttttcc catacgattt tttctgggaa aaaggtcgga
    19441 ggataacgaa cgatttcttc cattggtttc aatgaagtaa acaccatcca aagcaatgga
    19501 atgatcaaaa tgattcctat tactgtcatg aggacataac ttagtatttt ttttgtttgc
    19561 cgattcacac tggtcttttt gaaataatcc atcatttgcc gtcacctcca gaataataga
    19621 cccacttagg tgcaagcttc aaattgacaa acgtcagaac caacacgatc agaaacagga
    19681 tcattgacat agccatcgca taacccatat caaaagctac gaacgccttg ttccacatat
    19741 gcagattata aaaaagcaaa gaatttgcag gtccgcctgt tccatttttc gtcatgacat
    19801 aagcttcttg aaaaatttgg aaagaaccga tcgtagaggt gatcacatcg taaaaaataa
    19861 taggagtgat taatggcaaa gtaatacttt tgaactttcc ccaaacgcct gctccatcga
    19921 tttctgctga ttcatataaa gatctaggaa cattttgtaa tgtagctaag taaagtagca
    19981 ttgctcctcc agaactccat attttcatca aaatcaacgc aggtttcgtc cattgtggat
    20041 caaacagcca agctggtcca gggatatgga accagcccag aaatgtgttg atcaatcctg
    20101 cagatggaga aagcagctgc atccataaga tgtagactgc tactcctgat aagactgccg
    20161 gtaaatagaa agctgtacgg aatacactga ttcctttgat tttttgattg agtactgtgg
    20221 ctaaaaacac actgaaaatc gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt
    20281 tgtataacga tttccaaaat aaatcatcgt gaacgaacat tcttttgaaa tttgctagac
    20341 caatgaaatt catcttggat gtcaaattat aatcggtgaa actaccaata actgaaaaaa
    20401 gcataggccc tagcataaat accaagaacc cgataataaa aggcgagata aataaatagg
    20461 cccatcttgc ttcttttttc tttcggatag ataaacgctt caaattttct tgcctcctct
    20521 gtacctagac agtcaaaaat ggattattga ttttgcttga ctaagttttc tactgctttt
    20581 tgtgcatctg ccaatccttc ttttggtgat tttgttccta aaagaatctg gtcaatctgt
    20641 tcattcacta atgtcgtgta atccggagca tctaaaggaa ctggtgtgat caccgtattt
    20701 ttaaagtttt cgtccgccat ttgatagatc atttttcctt tatccgttag gttgtcatta
    20761 ttcactaaat tctcattggc tgtcttgctc gccatgatat caaaactttt ttcgccgaat
    20821 ttttcttgta cttctggaga agaaagatat ttgatgaatt cataagaggc ctcaggatct
    20881 tttgctccgt atggtacttc caagacgaat ccgccacccc acgaccaatg gcctgaacct
    20941 tcttcttttt ctgggagctg catcacgcca aaatcaaaat catccggtgc attttccatc
    21001 aaactagagt aataattgat attttgggca cgcatggcta ctaatccgga aataaatgga
    21061 tcagatacac cagacccaaa ttcggcttct aatttattga tcgtattttt cccataataa
    21121 tcttgccagt cttggatcca ctccaaggct tccactttat ttttagtatc aatcttgact
    21181 ttttcatctt tgtcaaacca actagtcccg ttatctgcgt ttagcgccca tacatcagcc
    21241 cctaaattcc ataatggata aaaaccgatg cgtgtaaatt cttttccttc ttttttatct
    21301 agtttacgtg ctgtttcttc taattcatcc caagttgtcg gtagatcttg ttcactgatt
    21361 ccagcttctt taaataactt tttattataa aaaatgactt gtgtatctgt attgaatggg
    21421 atcgcataag gatcaccttc atattctact gtatcccaaa gctgcggata aaaatcctta
    21481 gaagtgtctt tttctacata tttggagaga ttagttgctt gctgtgcttc tgctctttgg
    21541 gcaacggaat ttatgtcctg tacaataaca tcgggtgcat ttccagctgt tattgcagat
    21601 aaagatttag tccagatgtc accccatggc tgatacttat aatttacatg gatctcgttt
    21661 tgtgattgat tgaaatcctc aatgatttct tcgatagctt cttttcgtgc accggatccc
    21721 caaaaggacc agaaatcaat attttttctt ccatcacttg ttttttcaga agaatttgct
    21781 gcacttgaac tggcatcgct tgcattactt gatccgcatc ctcctaataa aaacaatgct
    21841 gaacttgcca gcaaccctgt aaacaataat tttttcatat tttacctcca aattttaatg
    21901 taaccgatgc cggttttaat ataacgatat cattttgttc
//
LOCUS       DAEGIQ010000041        21868 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000041 DAEGIQ010000000
VERSION     DAEGIQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.041"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(266..466)
                     /locus_tag="KMT45_001390"
     CDS             complement(266..466)
                     /locus_tag="KMT45_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE7004860.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="KMT45_001391"
     CDS             complement(771..2003)
                     /locus_tag="KMT45_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBE7004861.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2258..2827
                     /locus_tag="KMT45_001392"
     CDS             2258..2827
                     /locus_tag="KMT45_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE7004862.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3005..3445)
                     /locus_tag="KMT45_001393"
     CDS             complement(3005..3445)
                     /locus_tag="KMT45_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBE7004863.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3603..4367
                     /gene="map"
                     /locus_tag="KMT45_001394"
     CDS             3603..4367
                     /gene="map"
                     /locus_tag="KMT45_001394"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBE7004864.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4399..5322
                     /locus_tag="KMT45_001395"
     CDS             4399..5322
                     /locus_tag="KMT45_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBE7004865.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5398..6537
                     /locus_tag="KMT45_001396"
     CDS             5398..6537
                     /locus_tag="KMT45_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBE7004866.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6530..7330
                     /locus_tag="KMT45_001397"
     CDS             6530..7330
                     /locus_tag="KMT45_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBE7004867.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7330..8157
                     /locus_tag="KMT45_001398"
     CDS             7330..8157
                     /locus_tag="KMT45_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBE7004868.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8135..8869
                     /locus_tag="KMT45_001399"
     CDS             8135..8869
                     /locus_tag="KMT45_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBE7004869.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8969..9835
                     /gene="rfbA"
                     /locus_tag="KMT45_001400"
     CDS             8969..9835
                     /gene="rfbA"
                     /locus_tag="KMT45_001400"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBE7004870.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9849..10421
                     /gene="rfbC"
                     /locus_tag="KMT45_001401"
     CDS             9849..10421
                     /gene="rfbC"
                     /locus_tag="KMT45_001401"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBE7004871.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10443..11471
                     /gene="rfbB"
                     /locus_tag="KMT45_001402"
     CDS             10443..11471
                     /gene="rfbB"
                     /locus_tag="KMT45_001402"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBE7004872.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11569..12420
                     /gene="rfbD"
                     /locus_tag="KMT45_001403"
     CDS             11569..12420
                     /gene="rfbD"
                     /locus_tag="KMT45_001403"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBE7004873.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12455..14488
                     /locus_tag="KMT45_001404"
     CDS             12455..14488
                     /locus_tag="KMT45_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004874.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14532..15812
                     /locus_tag="KMT45_001405"
     CDS             14532..15812
                     /locus_tag="KMT45_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBE7004875.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16022..16828
                     /locus_tag="KMT45_001406"
     CDS             16022..16828
                     /locus_tag="KMT45_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7004876.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16840..18060
                     /locus_tag="KMT45_001407"
     CDS             16840..18060
                     /locus_tag="KMT45_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7004877.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18050..19636
                     /locus_tag="KMT45_001408"
     CDS             18050..19636
                     /locus_tag="KMT45_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE7004878.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19675..21813
                     /locus_tag="KMT45_001409"
     CDS             19675..21813
                     /locus_tag="KMT45_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBE7004879.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6897 a         3706 c         4546 g         6719 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccat tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt
     2101 tattttttta aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag
     2161 aaaatcaaaa actaattttg catatcaaaa aaaacatgtt acaatgaaat taatcaatgg
     2221 tggaagcgtt ataaaaaaat taaggagatt aaaaaatatg gcaagaaaaa agacgattac
     2281 tagagaacag attttggcag ctgcttatga agtagctgcc accgatggat ttacacgatt
     2341 tactgcgaga aacatcgcga acaaaatgaa ttgttccacg caaccgattt atcttgagtt
     2401 taaaaatatg gatgacttga gacaagctct ttttgagcag atctatgatc atttacaata
     2461 tgaagtgttc actgttgagc atacagggaa tacgattatc gatcttgctt ttaattatat
     2521 ccattttgca agaagagaga agaagttgta tcgttcactg tacttagaag aatatggtgg
     2581 aggaaaagag atgcagaatt tctcttatac ttactttgtg aatacagtga aacgcgatcc
     2641 agcttatgtg aatctttcag aagagcagat cgaatctttg tataatggga catggatcat
     2701 agctactggg atcgcagctt tgatgtcttc aagcatcatc catccaactg atgaacagat
     2761 tgaaaagatg atccaagaca gtatagatgc tatcttgaaa ttcaaagaac caatcgaaat
     2821 cgattgaata tactcaaaag ttccacaaat cagtaggtaa caagagatgt gtcaaaataa
     2881 ggaaaagttc gtttttctta cagcgtatgc atttttaact cccgaagaac tcttccatga
     2941 atgcaatgaa taaaagagcc atacagattg aataaatctg gatggctctt ttattgcaag
     3001 tatattaaat ttttgcgact agttctttag cgaaagcttc taaattttcg atatcttctt
     3061 cttcagcatt cagatccact ttaacatttt cggctccttt tgatgcgcct gtttttgcaa
     3121 aagcagcatc gaaatcatcc accgatttgc agaattcatc gtaaaaagta tcaccagatc
     3181 cgcagacacc gtaaattttt cccaatagat ccaattcttg caagtcttca taaaagtcta
     3241 cgatctcatc aggcagatcg ccatcatcgt atgtataagt agctactacg caaatatctg
     3301 cctcctcgaa ttcatctgca tctacttggg tacattcgtt gatttcgact tcgatatcca
     3361 gattttcaaa tgcttctgca acaatatcgg cgatttcttc agtgtttcct gtcatgctag
     3421 catatacgat ttttgctaaa gccatgatgt gcctcctcgt ttataattat ttaacaatta
     3481 tgaattatac caaaacatca ttgaaaagaa aataaaacct aaagacacct ggggaatgtc
     3541 atttggctaa atgtatgccg ctatgttaaa atgagaggga tttgtaaaaa ggagacgaaa
     3601 atatgattac tttaaaatca gcacgtgaaa ttgaaatgat ggcagaatct ggagctttat
     3661 tagcagatgt acataaaaat ctgcgtgatt ttataaaacc aggaatcaca agttgggata
     3721 ttgaggtatt tgtcagaaac tatatcgaga gtcatggcgg aattgctgcg caaattggct
     3781 ttgaagggta tgaatatgcg acttgtatca gtataaatga tgaaatttgt catggattcc
     3841 ctcgtaaaaa gcctttgaaa aacggtgatt tggtaaaagt cgatatgtgt atcgatttga
     3901 aaggcggcat ttctgattct tgttgggcct atgtagttgg agaatctaca cctgagatcg
     3961 accgattgat ggaagtaacg aagaaagctt tgtacatcgg tattgagcaa gcacaagtag
     4021 ggaatcggat tggtgacatc ggtcatgcta tccaaactta tgtagaaggc gaaaatctag
     4081 cgatcgttcg tgattttatt ggtcatggcg ttggtcctac gattcatgaa gaaccagcag
     4141 ttcctcatta cggagaagct ggaaaagggt tgcggttgaa agaaggcatg gttatcacga
     4201 tcgaacctat ggttaatacc ggaacttgga aaatgaaaat ggatcctaac ggctggactg
     4261 cgtatacaag agacggcgga ctttcttgcc aatatgaaca tacattggct attactaaag
     4321 atggtccaag aattttgaca tcacaaggag aagaaggcac ttattgatct atccagtcaa
     4381 taggtacggg agaggtacat gaaagtttgg aacaaaataa aacaaaatga aaatctgatg
     4441 cgctttattg agacgactca aaagcgcatc actgattcag aaatgagtac gacctctgtc
     4501 gtcgttgctt attatttact cttgtcttta tttccattga ttattgcttt tggtaatatt
     4561 ttaccgtttc tccacatcga tcaagagact gtactgacct atttacgaga agtgattcct
     4621 gagaccgttt accagtttat cggtcctgca atcaaagacc ttttgacaca aagttcagga
     4681 ggcttgttat ccgtttctgc attagcggct ttatggtctg caagtcaaag tatcaatgct
     4741 ttgcaaattg cgatgaataa agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc
     4801 cgtttcttct cactaattgt cattgtcttg ttcatgattg ctattagtgg ggtaactatc
     4861 gttcttgggt tgggacagct gattttggat gctattcagc caatcgtaca aatacccgtg
     4921 aactttatcg atcagttcca aactttgaag tggccaatca cattggtagc tttatttgtc
     4981 atcatgttct tgatttattt aattgtccct aatgctcagt tgaagttgaa gacgatcatt
     5041 ccgggcacga tcttcgctac tgtcggctgg atgctgttat cacaagtgtt tagtatctat
     5101 gctaggtatt ttgcaacgag gatcagcggt tatcagatca taggaagttt tattgtactc
     5161 atgctttggc ttaattttgc tgctacgatc atcattttgg gcggaatcat caatgcagtt
     5221 gttcaagaat acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg
     5281 ttcaataaat tgaaaactaa attttctcat aaaaaaaagt agaaacaaga gttcttattg
     5341 taaatttata tcagtaattt ataataatta tgactcttaa ttaaagtagg tgcccatatg
     5401 cttgtttctt ttagtatggt tataagattt tttctgacaa ttttattatc actgatatta
     5461 acacctgtat tcaaaatcat ttcagtacaa acaggtatgg tcgacaaacc aaatgaaaga
     5521 agaataaata aagttccaat gccttcagcg ggcggactgc ctatctttat ttcatttgtt
     5581 atatcgactt tattcttatt ccgtaaaatt atccctcaat cgtatattgt accgatcttg
     5641 attgcttctt tagtaatcat agtcacagga gtgctcgatg ataaatatga gctgacacct
     5701 aaacaaaaaa gtatagggat cttgattgct tctcttatta tttattttgt agcagatata
     5761 cgtattgatt ccttcacgct tccttttatt ggatatatcc aactaggctg gcttagtttt
     5821 cccgtaacga ttttttggat cttcggtatc acgaatgcgg tcaatctgat tgacggtttg
     5881 gatgggctgg cggcagggat ttcattgatc ggtttaatta cgataggaat cattggatat
     5941 tttttcctcc atgcttctac agtgtatatt ccagttgtta ttttctgtct tgtagcaagt
     6001 attatcggtt tttttcctta caatttttat ccggcaaaga tttatttagg cgatacagga
     6061 gctctattcc tagggtttat gatggccgtc ctttcactac aagggctgaa gaacgtgact
     6121 tttgtttcat cgatttcttt gctgatcgtc atgggagtgc cggtaacaga cacgtttttt
     6181 gctatcatta ggagaaaagc aaaccgtgtt tctttttcta cagcagataa aaaacatctt
     6241 catcaccgac taatcagttt aggctttact cataaaggtg ctgtcctgac gatttatgcg
     6301 atggctttga tgttttcttt cacagctatg gtcatgaatt atactggaag aatcggaacg
     6361 atcatattga ttattgccat gttgtttgct gcaattttat tatttgaatt gattggtctg
     6421 atcaatgaga aacatcagtt tattttaaaa acattgcgat ttttagggaa taaagagtac
     6481 cgtacgcaag taaaacagaa atatttgaag aaatggaacc gtcatataga tgaataacaa
     6541 attaaaccag ccgatcctta ttagtattgt gacggcaaac agtgataaaa tttttcggac
     6601 actagataat tttatttcaa ctgtaaaaga tgaaacagca gtaaaaatcc gaatttttga
     6661 caacaattca cttcccgagt acaaaaaaag actaacagag tataggaaat atccctacat
     6721 cgatatctat tttcatgatg aaaattccgg atttggttat ggacataatc acaatctgat
     6781 agctagcgat tctcgctatg gcattatttg taacccagat attttagtgg atgaagagac
     6841 agttacagca ttggtcttgt tattgaaaga acatccggaa tgtgcgatgt tagcacctaa
     6901 gattcttaat gaagatggaa caactcagca cctcatccgt gagcagctgg ctgtttttga
     6961 ttacgttttg cgctttgtgc ctttcaaatg ggtcaaaaaa aagtttgaca aacgactggc
     7021 tgtgtttgaa tgccgaggat tgcctactga tgctaatagt tatgtacgga tgatctcagg
     7081 tagttttatg gtagctgata ttgataaatt taaagagata aaggggtttg atgagcgttt
     7141 cttcatgtac tttgaagaca acgacttgtg tttgagtttt gaaaaagcag gttacaagtt
     7201 attgtacaca cccttttatg cagttatcca tttttacggg aagggtgcgc atcgaaatgc
     7261 aaaactgttt cgcgtgtttt tgacttctat gatgaaattt ttcaataagt gggggtggcg
     7321 ttttttctga tgaaaccaga aatattagta gtcgttgtgc tttatcagca actattcagt
     7381 caatctccag cttatggacc attaagcaaa gcacttagcg aaaagcaaat tcagcttatt
     7441 atttacgata atagtcctca aaggcagcaa aatgagctgt ttgaaaaaga aaatatcgtt
     7501 tatcatcatg atccgcaaaa tcctggttta gcggtggctt ataattttgc gttgtctcaa
     7561 gcagatgaag gaacacgttg tctcgtcact ctggatcagg atacgaggct agtcgacgat
     7621 tattttgaac tattgcgtaa agtaaccttt acagatgaat gtgttgctgc agttccgatg
     7681 attttttctg ggagccgaca agtttctccc ctatattcag atcgttatat caatcgtaca
     7741 gctcaagcag tagaagttga aggcccaaca gctgaacgaa tcatggcaat caattcaggt
     7801 gttgcctggt ctgtcaaatt tctaaaaaag attggcgggt ttaatcctgc tttttcttta
     7861 gattttttgg atcattggct gttttggaaa gtggatcagt tggaaaaaac tgtggagatc
     7921 cttcctgtac gaatggaaca tgatctttcg gttttagact atgaaaaagt ttcaagtaat
     7981 cgataccgtt ctattttaca agcagaaaat cgattttatc atgaatatga tcaaaggcat
     8041 ttatccagtc accgacgaca attattgtta agaacagcta aacagttctt gaccgtcaaa
     8101 aaccggcata tctggcggat gactttgcgc tcatatgtta agaattggaa ggtgtgaatt
     8161 cgtgcgttcg gtttgtatag ctacctttaa tggaggcaga tatttaagag agcagttaga
     8221 tagtatttta tctcagcttt cagcagaaga tgaagtagtc atttctgatg atgggtcagt
     8281 agatgataca tgggatatat taactgaata cgcagaaaaa gataagcgaa tccagctttt
     8341 ttcggggcct cgtcaaggat tgatcgctaa ttttggttat gcgatccagc agacgaaagg
     8401 agatctaatc tttttggcag accaagatga tgtttggttg gagacaaaag tgaaaaagat
     8461 cactgactat tttgagcaga atccggatca gctagtagta gtttctgatt taatcattgt
     8521 ggacagcgag ctgcaagaga tccatgattc gtatttttcg tttcgaaatt ccaaagaagg
     8581 atggcttaat aatgtgataa gaagtcacta tattggtgct ggtatggctt tccggtcttc
     8641 tttaaaaccc gatattttgc cgatcccgtc agaagtgccg atgcatgata tgtggattgg
     8701 tttgctggga ggaaagaaga ctgggttcct aagagaaccg ttaactctgt accgaagaca
     8761 tgatttaaac gcgagtgaga ttgaaacaca ttctaaatta cggcaaaaaa tttcttggcg
     8821 tgttcatctg attacggcgt tgatcaaacg aaaatggttt aaacgttgat aaaagatgtt
     8881 taaatcctta atatttccat gacaaatata acaattgatg agataataac aaagtgaaat
     8941 ttgaaatcaa aaaggagagc agatatccat gaagggaatc attcttgctg ggggcagcgg
     9001 aacacgtctg taccctttaa caaaggcaac atcgaaacaa ttgatgccga tttatgacaa
     9061 accaatgatt tattatccaa tgtctactct gatgttggcg ggaatcaaag aaattctgat
     9121 tatttcaacg ccacaagata caccacgttt caaggaatta tttggaaacg gaaatgattt
     9181 aggactgcag atcgaatatg cagtacaaga aagtccagat ggattggcac aagcgtttat
     9241 cattggagaa gaatttattg gagatgatag tgtctgcttg gtattagggg acaatattta
     9301 ctatggtggc ggtctgtcta aaatgttgca acgagcagct tcaaaagata caggcgcgac
     9361 agtatttggc tatcatgtca atgatccaga acgctttggt gtagtagaat ttgatgagaa
     9421 tatgcaagct ttgtcgattg aagaaaagcc tgctcagcca aaatcaaatt acgctgtaac
     9481 tggcctttat ttttatgaca atgaggtaat ttctatcgct aaaggtatca aaccttcaga
     9541 acgtggagaa ctagagatta cggatgtcaa taaagcatat ttggataaag gaaaactatc
     9601 cgtagaactt atgggacgcg gatttgcttg gcttgataca ggaacacatg aatcattgct
     9661 tgaagcatct acctttatcg aaacaattga aaaacgtcag aacttaaaag tggcttgttt
     9721 ggaagaaatt gcttaccgta tgggctacat cgataaggaa caattgctgg cccttgcaca
     9781 gccattgaaa aaaaatcaat acggtcaata tttattgaat ttggccgctg aataaggagt
     9841 ccttacagat gaaagtaaca gatacaaaat taacagatgt caaaatcgta gaaatggacg
     9901 tctttggcga tcatcgcggt ttcttcacag aaagttattc aaaagcaaaa tttgcagaac
     9961 atggattgaa atatgacttt gttcaagaca accattcact atcagctgaa gctggtgtcc
    10021 ttcgggggct tcatttccaa aaaggagaag ctgcacagac aaaattgatt cgggtagtca
    10081 ctggagcagt tttagatgta gtagtggata tccgcaaagg tagcccgact tatggtcaat
    10141 gggacggatt tatcttatct gaacataatc atcgacagtt gcttgtacct aaaggatatg
    10201 cccatggatt cgttactttg acaccaaatg tgaacttcct ttataagtgt gataattact
    10261 ataatgcaga agcagatggg ggcatcgcat ttgatgatcc agatttagcg attgattggc
    10321 caattgatcc atcaagagcg attatgtcgg aaaaagatca ccgtcatccg acattaaaag
    10381 aatttgaagc agaaaatcca tttgtttacg gagaaatcta agaattaagg agaaattttc
    10441 ttatgaaaaa catcattgtt accggaggtg ccggattcat cggctctaat ttcgttcatt
    10501 acgtggtaaa caatcatcca gaagtgcatg tcactgtttt ggataaactg acttatgcag
    10561 gaaacaaaga gaacttggcc ggacttccta gcgatcgcgt agaattggtc gtaggagatg
    10621 ttgcagatgc agaattagtg gaccgtctag taaaagaagc ggacgcagtt gttcattacg
    10681 cggcagaatc ccataacgat aattcattaa aagatccttt cccatttgta caaacgaata
    10741 tcattggtac gtatacactt ttagaagctt gccgcaagta tgatgtacgc tatcatcacg
    10801 tatctactga cgaggtttac ggcgatcttc cattacgtga agacctgcca ggacatgggg
    10861 aaggcgaagg agaaaaattc actgcggaaa caccatataa tccttcaagt ccatattctt
    10921 ctacaaaagc tggttcagat ctgttagtaa aagcatgggt gcgttctttt ggcttacgtg
    10981 caaccatttc aaactgttca aacaactatg gtccttatca gcatattgaa aaatttattc
    11041 ctcgccaagt aacgagtatc ttaagcggta ttcgtccaaa attatatggc gaaggaaaga
    11101 atgttcgtga ctggatccat acgaatgatc attcttcagc agtgtggctg attttgacga
    11161 aaggcagaat tggcgaaacc tatctgatcg gtgcagacgg tgaagaagat aataagaccg
    11221 tgatggaatt gatcttagaa atgatgggcc aaccaaaaga cgcatacgac catgtaaacg
    11281 accgcgcagg gcacgatttg cgttatgcaa tcgattcgac aaaattgcga gaagagctag
    11341 gttggcagcc tgagtttacg aacttccgtg atgggttggc tgacacgatc aaatggtacg
    11401 aagaacatga agactggtgg aaaaaagaaa aagaggccgt tgaagcagca tatgccaaaa
    11461 atggacaata atacagagga aatttcactg tattaatgga aaaacacatg tgaggcaggc
    11521 gctcgcctgt ctctttttta aaataaaaat caaggagcgg ataaccatat gtttttatta
    11581 actggaggaa atggacaatt aggtacagaa ttgcgccatt tattagatga aaaaaatctg
    11641 ccatatgttt caacagatgc aaaagacttg gatatcacag atgctgagaa aacaatggct
    11701 tatattacag aattaaaacc cgatgttatt ttccattgcg cagcttacac agcagtagat
    11761 aaagcagaag aagaagcaaa agaattagat gagaaaatca atgtcgatgg cacaagaaat
    11821 gtagcacttg cagcaaaagc tgtgaatgca caattcgtct atatcagtac agactatgtt
    11881 ttcgatggaa aaaacaaaaa tgtagaatac gaagtagacg accaaacgaa tccactaaat
    11941 gaatatggtc gtacgaaatt attaggcgaa aaagcagtag aagagatttt agaagactat
    12001 tatatcatac gaacttcttg ggttttcggg atctatggac acaatttcgt cttcacgatg
    12061 caacgcctag cggaaacccg cgacaaactg actgtagtaa atgaccagtt tgggcgacca
    12121 acttggacac gtactttggc agaatttatg gtatatgtca tcgaagaaaa agcaccattt
    12181 ggtatttatc atttgtcgaa cgaaaatagc tgttcttggt atgagttcgc aaaagaaata
    12241 ttgaaagata cagatgtaga agttgcacct gttacgtcag aagaatttcc gcaaaaagca
    12301 acacgtccgc agtattcagt catgagcctg aaaaagacag aagcgttagg ttttgtcata
    12361 ccaacttggc aagaagcact tgcgcaaatg ctggaagctg ccaaaaaaat gaataagtaa
    12421 tttccttttt atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg
    12481 acgaaaagaa aagccaaaag cgaatcaggc agattcgaat ttggtgcttg acctgtttct
    12541 aaaactccgc tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa
    12601 cggaagttca cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac
    12661 tgatgtgatt tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc
    12721 tttttacctc tcacaagctg aagaggactt ttcgttggtg aacgaggatt attcattaaa
    12781 cggacaaaac atgatcattg cgtataattc tcctgtaaag gatattacgg ttatcggaaa
    12841 accattcaac tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc
    12901 gatgaaatta gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag
    12961 gaaaaaagct ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg
    13021 gtggttcatg caacatgtgg gcgatttgat tttctttact ttgggactga tgattgcttt
    13081 ttatcattat ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt
    13141 ggttattttc ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc
    13201 gtacctgctg attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg
    13261 ggatctgttg gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt
    13321 tcatttctgg ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg
    13381 aaaacgggta agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa
    13441 ttggaaaatt ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta
    13501 tcactttttt ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag
    13561 cgaacaaaag ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat
    13621 taccgttggg ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt
    13681 aagagccatc cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta
    13741 tatttggaga gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc
    13801 atggtcttat ttctatgcga ttacgcattc gaaaatggat aattattttt cggattttga
    13861 aaccatcgca gttgctgtgt tgggaacagt gattttagct tttgcattat ttggctggag
    13921 acgcttgttt tacctcgctt ttgtcgcctt agttatcgta tctggctttt tcgtcaatcc
    13981 agtcgtagaa ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat
    14041 cgatcaacaa gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc
    14101 aacattggga gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa
    14161 gaaaatcgat ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc
    14221 ttttatcgct aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac
    14281 gttgaatttt gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt
    14341 aactgattac aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga
    14401 cggttatatg atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac
    14461 tcaggaaaat cagctagtcg tacagtaata ggctagctga gaataatcct gaaatattag
    14521 tagaaaggag agtgaagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg
    14581 tcggtttctt gcttttgttg ctgacgctag tcttttttgt ttggacagca ggggtaggtt
    14641 ttccagcaac tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta
    14701 ttttgctcat tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg
    14761 cgcttgccct tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg
    14821 gagacttact gtttcctttg attattttat tttgtttagt aattaaatgg atcgagtgga
    14881 caaaatggga tcgcggtatt tatctgattg tctttacggt cttcctcgga atcacgttat
    14941 atcgtgtatt tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt
    15001 cgctttctga tatttggatc aatacgaata caatcggaag ttctttgatg actcttgctt
    15061 tattgatcag tggatttgcc agttcttttg aacgatggta tgttcgaatc atcggtggga
    15121 tagcagtcgt tgcagccttt ttaggtattt gggtctgcca atctagaggt gcattagtcg
    15181 cgttgattgt ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg
    15241 cgccatttat tgcctacacg gttacgattc ttttagcttt gccgatttct tatctggcgg
    15301 ctgtctctga aaaagtcaat ttgtttaccg gtcgagaaga tatttggcat aaattctatc
    15361 agacgctagg agaaaaatct gaacaagttt tactaggaat gaaaacattt atcttccagc
    15421 gaggaaacca atttttagga aaccacaata gttataattc tattttgaat atctatggat
    15481 tgataggatt tgggattgct gctttgctgc ttatcctttt tattggtcgc ttaacgttga
    15541 aagcagattt atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc
    15601 agtcatttat ggaggataca ttgacttcat accgatgggt tccgattatt tatatcctgt
    15661 tggctatggc cgttcatcga tatgatgaac cagaagttac ggaggaattc tatgttatgg
    15721 aagaggcata tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg
    15781 ctcattcttc caggatccaa cgctatcatt gataggcaca aaatacgaga gaggctgtga
    15841 caaccgtcgc agcctttttt ggtttattgc aattgtctag catcttctgt aaaatagaaa
    15901 cgttagagaa aatcttgtat catagaaaat taagaaagaa ataagaacgc agctcggtac
    15961 caaaaatatg gtaaaatggc acttgactgt aattcactga aaatcgcttg gaggaacaca
    16021 aatgtttaaa gatttgtatt tatttatgaa ggacatttat cagaacagaa aattactgct
    16081 acagttttcg atcaatgact tcaaatcaag atatgcagga tcgtttttag gaatcttatg
    16141 ggcgtttatc aatccgctat ttactgtatt gatttattgg ctggtatttg gtgttggact
    16201 gaaagcacaa atgatcgatg caaaataccc atttatcgta tatttagtta ccgggatgat
    16261 tccctggttc ttcttctcag atgcatttgc atcaactacg gttgtttttc gggaatatac
    16321 ttatttagta aagaaagtcg tattcaatat tcgtatcttg cccacgacga aaatcctatc
    16381 caatctgtac acccatttat tcttcattct gattggcata gctgtcgcgc ttataaatgg
    16441 tatatatcct tcagcaatgt ctttacagct gatttattat ttattttgca tgtgtgcttt
    16501 cttgacagga ttgacttggc tgacggcatc gatccaacca tttttacctg atatcatgca
    16561 attaattacg atcgtgctgc agttgatcat gtggacatta ccgatattat ggagccctaa
    16621 tcagtttgat tcaggtcttg taagcatatt gaaattaaat ccgctgtact atgttgttca
    16681 aggttatcgt gagtcatttt taagtgaagc ttggttctgg gagcatggaa catatacttt
    16741 gtatttctgg gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt
    16801 gaaaccacat ttttcagatg tgctgtaagg agaatagcta tgacagaata tgcaattaga
    16861 ctaaaagata tcacaaaatc ttataatatg tacgcaaaac cttcggatcg atttcgtgaa
    16921 gccctcaatc cttttaagaa atcctatcat gatgtatttt atgcgttgaa aaacgtgaat
    16981 gtagaaataa aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc gggaaaatct
    17041 acaatgttga aaatcattac gggagtactg acaccaacat ccggcacgat ggaaatcgaa
    17101 ggaaaaatct cagctttgct agaactagga tcaggattca atcctgagta ttctggctat
    17161 gaaaatatct atttaaatgg aatggttcta ggattttcaa gagaagaagt cgatgaaatg
    17221 gtcgatgata tcattgaatt tgctgacatc ggcgaccata tttatcaacc tgtaaaaacc
    17281 tattcaagtg ggatgttcgt tcgtctagct tttgctgtag cgatcaatgt gaatcctgat
    17341 attttgattg tggatgaagc attggcagta ggagatcttg aatttcaact aaaatgtatg
    17401 gaaaaattta cagaaatcaa gaactcggga aagacgattt tattcgtttc tcatgatatt
    17461 agttcgattc gccgtttttg tgatcgttcc tattggttga aaaatggaaa agttgtcgaa
    17521 tatggtgata cgatggaagt aacgacgaat tacgaaaact ttttgaagaa aaaatcagtt
    17581 aaaacagtag atcgaaataa aaaccaagta gaacgcttcg cagctccaga tatcgtagac
    17641 gtgacgaaag ccgaattgtt ggataaagat aaaaatccag tggatatgct ggaacaagga
    17701 gaaaaactat acgttaaagt aacctatgaa gtaaaagacg atacaatcaa aaatccagtt
    17761 ttaggaattg ctctgcgtac agtcgacaat ttttatgtgt gcggactcaa tactctacta
    17821 gatggtatca agatcccttg gaaaaaaggt gagaatatct tttatctgga gtatgaaaag
    17881 ttaggtttgt tagctggtga atattatttt gacgttgcgg tctttgaaga aaatgcaacg
    17941 gttcctttgg tatacaagac aaaatacatg aacttgtttg tcagcggttc ttatatcgga
    18001 gaaggcatcg tggtcttaga ccataaatgg gaagaaggga ctcatagcaa tgaaatatga
    18061 ttttgaaatg gacctagatg aacaaagcag tgtgggaaaa attgccgcac agatcaaacc
    18121 aggaagtaaa gttttagagt ttggtcctgg aaacggccga ttaacgaaac atttgatcgg
    18181 agcaaaacaa tgtgaagtga gcattgtcga gctagataag gaattgttcg attttgtcag
    18241 cgagtttgcc caagatggat tttatggaga tatcgaaagt tttgaatggg caaactatta
    18301 tgctggacaa acatttgatt acgttttgtt tgccgatgtt ttagaacatt tggtagatcc
    18361 tggaaaaacg ttgaagaaag ttagagaatt tttgaatgaa gaaggagaaa ttctaattac
    18421 attccctaac cttgcgcata attctgtcat gattgatttg ttcaataatc aattgccatg
    18481 ggcttcttat ggtttgctag atgaaaccca taattctttt tatactcatg atggcttcca
    18541 aaaagttttt gaaaaagcag gattatacat caatattgaa gattatttgt acttagctgt
    18601 aggtgataca gaattaaaat caacttatga agaattgccg gaagctgtac gttatgattt
    18661 caaaatgcga ccatttggcg aagtctacca atacttcttc tcattgatga aacatccagt
    18721 agcacaaagt tccattgcgc agccgcaaaa ttctaattat gtgaaagtat tagaagtaat
    18781 ccagcaaacg aaacaagatg aaataattca acaaatacca ttcaataatt tcacagggga
    18841 aaatgagaca ctaagtttcc atgtatcaga attagcagaa cgaatggtgt ttcgcttcgc
    18901 gcagcaacct agctttatcg aattcagcgc agaagctgct ggagaaaaac tcacatttat
    18961 cgattcaaat gctgtagtca aaacagtaaa tgattgttat ttgtttgatg gtaaagattt
    19021 accagagttc tttttaacgg atattgcagg gaaagaagtg actatccatt gtcactatcg
    19081 ttttatcgga gaattgacac caacaatgaa agaattgctg gaaacaatcc gtccaatggc
    19141 tgaagttaca aagcagcttt ctgagaaaaa tgatgaatta gaaagagaaa atcattatct
    19201 tattgagaat aatacaagat tagatcagac gttgaaaaca accataaatc gctattgcac
    19261 gttgttggaa agtgatgaat ggacaatcaa acatagatta gggcgacgta agaaagaaac
    19321 atcgaaaaaa atccaagaaa aagagctttc tctatgtatt gacgaaaaaa tctgggatgc
    19381 agaaacaaag atattgaaaa tcatcgggtg gggaatcgct aattcggatc gccaaccatt
    19441 gtcctataag ttatcagccg accaatcacc attttttgaa gcaattcctg ttttacgaga
    19501 agaagtcaat caagcaaaac agctagctga aggagcaaaa gcaggatttg aactgcggat
    19561 cctttgcgag aaggaacgtt cattcttagt tgaagcagtg gctcaaaatg gtcaatcatg
    19621 gatcattgaa atgtaaataa tgattaactt ataaagataa ggagtaaacg aatcgtgcaa
    19681 aaaaaagaaa ttgccgtatt tatcgatcaa atcactcgtg aacgggccac tggcgatttg
    19741 cttattgtgg gctgggcaat tgatgaagtg accaaagaaa tccctacgat aaaggtagaa
    19801 aaagaaaatg tgattgctga agcaacacat gtcgtaagat tggatatcaa tcatttatat
    19861 aatcttgatg ttaagaccca aagcggtttt aaggtacgtt tatcagggaa aatgagagga
    19921 aaagcaatct tagacttcca aacagcaaga catcaaaatg gcatagctgt gaaattaaac
    19981 ggtaaatatc cttatgatga cggcattgaa tcttcatggg aaagaaaaaa aagattactg
    20041 aaaaaaggga taaattatgc aaggactcat ggcgtaaaaa aagcaatccg ccgggtcaaa
    20101 ctggaattga aaccaggatc gatcgactat gcagagtgga tcagtcgtca tgagaaacct
    20161 gatctgaaag agcaaaggaa agaagtacaa ggatttgcct accgcccatt gatttccata
    20221 cttatgcctg tctataacgt tgaaatcaaa tggctcgaaa aatgcattga ttcggtactt
    20281 gatcaaacgt atgatcactg ggaactatgt atcagtgatg atgcttctac tgatccagct
    20341 atccgaaagt gtctagaatc ctatcaagca aaagatgata gaattaaagt cgttttccga
    20401 caagaaaatg gacacatcag tctggcaact aattctgcat tggaaatggc agaaggagag
    20461 tttatagcgc tcttagataa tgatgacgaa ttacctccat ttgccttata tgaagtggca
    20521 aaagtgctaa acgtacatcc agaactggat ttgatctaca gtgacgaaga taaaatcgat
    20581 gcagatggga atcgctttga cccgcacttc aaagcagact ggtctcccga tacattgatg
    20641 ggaaataatt atatctctca cttaggcgta tatcggacaa gcattgttaa ggaacttggc
    20701 ggtttccgta aaggatatga aggctctcaa gattatgatt tggttcttcg tgtgacagaa
    20761 caaattcctg cagaacacat ttaccacatt gaccgtgtct tgtatcattg gcggacgatc
    20821 ccgggatcaa ctgcaagcaa tggagaagct aaaagctaca tctatgattc tggagtgaaa
    20881 gcattgacag atgctctatc tagaagaaat atcaagggaa gcgtacatcc tggcagaatt
    20941 tctggttttt atgaaatcac ttatgatgtt ttgcaagaag acttggtcag tgtgatcatt
    21001 ccaactaaga atggctatga agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg
    21061 tcttatccaa attatgaaat cattattgca gataatggaa gtacagatcc taaaatgcaa
    21121 gaattatttg cagaatacaa gcatcagctg aaagaccgat ttatcgtcga attaattgac
    21181 attccattca attattcacg tatcaacaat ctggcagcag aaaaagcaaa cggcaaatac
    21241 tttttattct tgaataacga tacagaagtg attgaacctg actggatgac agcgatggtt
    21301 tcttatgcac agtttgatcg aatcggctgt gtaggagcaa aattatttta tccggatgat
    21361 acaacgcagc atgcaggtgt attattaggg atcggtggcg ttgcaggaca tgcattgaat
    21421 aactatgatc gaacacattg tggttacttt ggccgattgg tcattgacgt caattactta
    21481 gcagtcactg cagcttgtat gatggtcaag gcagctgact tcaatgctgt caatggattt
    21541 gatgaaatat tagaagtagc attcaacgat gttgacttgt gcttgaaagt ttatgaactg
    21601 ggtcgttaca atgtctatgc acatcaagca gaattgtacc atttcgaatc taaatcaaga
    21661 ggttatgaag atacaccaga aaaacaaaga cgttttgctg gggaaatcaa aaaaatgcaa
    21721 gataagtggc cttcttatat tgctcatgac ccattttata acgataatct aactaaagaa
    21781 ggaatcggcg atttttcgtt gagacctgat tgattaaaag aactgtattt tcatttttat
    21841 taatgaaaat acagttcttt taatttaa
//
LOCUS       DAEGIQ010000042        21675 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000042 DAEGIQ010000000
VERSION     DAEGIQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.042"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1174
                     /locus_tag="KMT45_001410"
     CDS             <1..1174
                     /locus_tag="KMT45_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HBE7004880.1"
                     /translation="GLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIER
                     SQTAVVELTKENRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGK
                     LAIDGLAPGTYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTK
                     IDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYE
                     LDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTV
                     LQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKE
                     NRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1598..1768)
                     /locus_tag="KMT45_001411"
     CDS             complement(1598..1768)
                     /locus_tag="KMT45_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7004881.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1962..2141)
                     /locus_tag="KMT45_001412"
     CDS             complement(1962..2141)
                     /locus_tag="KMT45_001412"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004882.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2528..3640
                     /locus_tag="KMT45_001413"
     CDS             2528..3640
                     /locus_tag="KMT45_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBE7004883.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3718..4011)
                     /locus_tag="KMT45_001414"
     CDS             complement(3718..4011)
                     /locus_tag="KMT45_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBE7004884.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            4179..4493
                     /locus_tag="KMT45_001415"
     CDS             4179..4493
                     /locus_tag="KMT45_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBE7004885.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4514..6493
                     /locus_tag="KMT45_001416"
     CDS             4514..6493
                     /locus_tag="KMT45_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBE7004886.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6506..6832
                     /locus_tag="KMT45_001417"
     CDS             6506..6832
                     /locus_tag="KMT45_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBE7004887.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6848..7366
                     /locus_tag="KMT45_001418"
     CDS             6848..7366
                     /locus_tag="KMT45_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004888.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7366..8718
                     /gene="celB"
                     /locus_tag="KMT45_001419"
     CDS             7366..8718
                     /gene="celB"
                     /locus_tag="KMT45_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBE7004889.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8802..10307)
                     /locus_tag="KMT45_001420"
     CDS             complement(8802..10307)
                     /locus_tag="KMT45_001420"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBE7004890.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10399..10989)
                     /locus_tag="KMT45_001421"
     CDS             complement(10399..10989)
                     /locus_tag="KMT45_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBE7004891.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            11168..12814
                     /locus_tag="KMT45_001422"
     CDS             11168..12814
                     /locus_tag="KMT45_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBE7004892.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12863..13582
                     /locus_tag="KMT45_001423"
     CDS             12863..13582
                     /locus_tag="KMT45_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBE7004893.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            14061..15008
                     /locus_tag="KMT45_001424"
     CDS             14061..15008
                     /locus_tag="KMT45_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7004894.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            15020..15940
                     /locus_tag="KMT45_001425"
     CDS             15020..15940
                     /locus_tag="KMT45_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7004895.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15981..17438
                     /locus_tag="KMT45_001426"
     CDS             15981..17438
                     /locus_tag="KMT45_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE7004896.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17783..18397
                     /locus_tag="KMT45_001427"
     CDS             17783..18397
                     /locus_tag="KMT45_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBE7004897.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18390..20114
                     /locus_tag="KMT45_001428"
     CDS             18390..20114
                     /locus_tag="KMT45_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBE7004898.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            20111..21562
                     /locus_tag="KMT45_001429"
     CDS             20111..21562
                     /locus_tag="KMT45_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7004899.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6998 a         3680 c         4416 g         6581 t
ORIGIN      
        1 cggtctaact acaggagaag atggaaaact ggcaattgac ggtttagcac caggagcata
       61 tcagctggta gaaactcaag cgccaatagg gtatgaatta gatgcgacac caatcgagtt
      121 tgagatcgaa agaagtcaga cagcggtggt agaattgacg aaagaaaacc gacttacacc
      181 aggcggtgtc gtgttgacaa agatcgatga ccaaagcggg gaaatcctgc aaggagctgt
      241 ttttgagtta cagaaccgag aaggagaaac gctacaaacc ggtctaacta caggagaaga
      301 tggaaaactg gcaattgacg gtttagcacc aggaacatat cagctggtag aaacccaagc
      361 gccaataggg tatgaattag atgcgacacc gatcgagttt gagatcgaaa gaagtcagac
      421 agcggtggta gaattgacga aagcaaaccg acttacacca ggcggtgtcg tgttgacaaa
      481 gatcgatgac caaagcgggg aagtcctgca aggagctgtt tttgagttac aagatgccaa
      541 tggaaccgtt ctgcagtcag gtctaaccac aggagcagat ggaaaactgg cgattgacgg
      601 cttagcacca ggagtgtatc agctggtaga aacccaagcg ccaacggggt atgaattgga
      661 tgcgacacca atcgagtttg agatcgaaag aagtcagaca gcggtggtag aattgacgaa
      721 agaaaatcga cttacaccag gcggtgtcgt gttgacaaag atcgatgacc aaagcggaga
      781 agtcctgcaa ggagctgttt ttgagttaca agatgccaat ggaaccgttc tgcagtcagg
      841 tctaaccaca ggagcagatg gaaaactggc gatagatggc ttagcaccag gagcatatca
      901 gctggtagaa actcaagcgc caacggggta tgaattggat gcgacaccag taactttcaa
      961 gatcgaaaaa gaacaagaag tggcgatctt tttaacaaaa gagaatagaa aagttgctga
     1021 tagctcagat ataagaggaa acaatccaac aagcagtgga aataagcatc ttccaaaaac
     1081 tggagaaacc atcattagcc agtttatatt gtcaatattg ggtgtgctac ttgttttcat
     1141 cagtataaaa tttagaaaga aacgaaatat atgagctaaa ataaaaaatt gaataagcat
     1201 agtttttaaa atcaactagt acccatcaat tgaatggaaa ctaccgattt tagaaggggt
     1261 atacaaacaa atattagtat cggagtagag aaggttgaac ccaaccagta aataagtaaa
     1321 gacaattttg tgctgcgttg agaagtgaaa aataaacctt tgtagtaata ctagttaata
     1381 aaaaatatac aaaaacactg cgaaaatcat tcttgagttt cgcagtgttt tttgcctaat
     1441 gactcaagtt tattgggtaa tactttgatt ttctttctac aaataatcag cgatcagttg
     1501 taattttttc tctagtttta atggctcttt gtcaataagg agaaaaacaa gtcgggtctt
     1561 ttgtctcgga aagcttcaac caactcgtga ataaaggtta cgcctccttg aaaggcgtgg
     1621 aaaaaccaag taatcgatca atcaccaatt tcgcatttgg tagaacatgt ttggtgagct
     1681 gaaagtaggt ggcgttcatg tctgtaacta agaattctat ttctttagga ttagtgcagc
     1741 taaggaaata gtttgttaat cgaggaattt tttgtgtagg taaattttct cgattaattt
     1801 ttctgtctcg ccatccgcgg aaataaaact cagtttatcc tcaacggaaa cgtgagaacg
     1861 aaattcacaa ccctcaacac tcaagacaga attcttttgg aagactttgg taaatagctt
     1921 ctcaactctt tcaatgtgcg aataacggtt gtcaaagata cctaacaact tttcgaaata
     1981 aaaaataacg acactttttc agtcagtaaa gaagtgattt tatgtctgac atgatttgaa
     2041 atcgagtgcc ttggacggat aaaataactt tgagcgttcc aatggcttcg acagttttta
     2101 caggtatagc gctgcttttt taggcgtata accattggca tatgattata ttattcaaag
     2161 tggacaatgg tttccttctt cttgtttttc accaccatta atttcctatt tccatctacc
     2221 acagcagaac cacaatttct acagacacat ggagcaggga agagaactgc agcaacgatc
     2281 aatgtttttt tcttctgtaa ggtttcgtaa gagacctctg taatcatcaa atttttgtct
     2341 attgatctca gaattttttt gatagaacca ttcataaagc atatcgttct ctcagttgtt
     2401 tattttgtgg tgatttaatc atacaagaaa acgatatgtt tttttataaa aacatgaata
     2461 ggaaaaatag ttgacaagaa aatgacaacg ccatatgatg ttccatagta tttacaacct
     2521 gtcatagatg actgtaaaaa ataaaagtaa aaagaaagga cgacaacagg tggattttta
     2581 tcagtctcta caactagatc cgtttatttt aaaacaaaaa atacgagaag cagcttctaa
     2641 aaaagaaaag tgcatgtata tttgttcatt gtttttacga tcattattca tcgtattgtt
     2701 tgccatttgt tttatcatta taattacaac attatttgaa tcgaagcata aaccatacgc
     2761 tgtggtttta ttctgtatgt tgatgagtat tcgttttgtt gactttggtt ataagatttc
     2821 ccattcgatc ataggtttag caatcgtcat gttctcacta ttagtagcac cgtatgtcca
     2881 gctgatcaaa tggtcagtta tgggcatgtt gattcatttt attttactaa gcagcatatt
     2941 aatggcaacc gctagtgatc ccaaaatggg gaatgcttct ttgtacggct tttcatatct
     3001 ctttattgtt tattcgttac ctaaagattc gttgaacaag aatttcttta cacaaacaag
     3061 tagcttatta tttttgtttt tttgttggtt ttcagttctt ctttacagaa aacatcgaga
     3121 aaagaacaaa gacaaaagct tgtttaaaga gatttttttg aaaggcatgt attcgcagga
     3181 aaaaatttgg atgctcatct atgcatttgg cattagtctt ttgatcgttg caggagagta
     3241 tgtccctttt cagcgattga tgtgggcagg gtttgcattt tcttctattg tatctagcta
     3301 tggattgatg tcgattggtt tcaaagaacg agcagtagac cgaatcattg gctcattgat
     3361 tggatgtgtt ttatttattg gtatcagcca atttattcct tttaactggg tcggtatatt
     3421 aggtggtcta gctttaggtg tttgttctac ctatagatat aaaacagttt ttaattcttt
     3481 tggtgctttg gcaatcactg ccagtttatt cggggttcca ggagcagtaa cgatccgcat
     3541 ttttgagaat atccttgggg tatgtttagg aatcatgtat attggggtga ctgaaatact
     3601 tatccgaaaa ataagagaaa aacacggttt gaatcattag atgagatcga ataaaaaata
     3661 aggaaaaagt gaagatacac atggatgata aattgctatc attccatgtg tatcttttta
     3721 cggtaaatca gtgaaacgtt cgactttcat ttttaattta tactttttac ggagttcgtt
     3781 tatttgagcc atcatttcgg atttgtgatg cttttcgatt gcttcttcat ctttccaacg
     3841 gtcgatcagt aaaagagatt ctggatcttc tagtggaaag taatagtcat atttctcgtt
     3901 tccttcttct gcacgaatcg ctttgacaat gtttctttcg accatttctt ctgcaaattt
     3961 tctggcttgt ccatttgtac ttgtatacaa aatattcact gtgatactca ctaaatcccc
     4021 tcctgaaaaa attctatcac tagtatatca cggtgccaga aattattatt caagatatga
     4081 tacacatttt ttccgcaatg ctacggaaat atatattttg aatagaaaac gcttacaaga
     4141 tatgcttggt ttataaaaat aaaaggagtg attgatttat gaaaaaagca ttgattattt
     4201 gtgcggcagg aatgtcatcc tcgatgatgg cctcaaagac aacggatttc tttaagcaaa
     4261 aaagagaaga ggtactcgta gatgcagttt cagctacaga aggcgataat atgattaaga
     4321 atagtgattt tgatttattt ctgatcagcc cgcaaacgac gatgtttttg gataaattca
     4381 aaaaattagg agaatctgtt ggaaagcctg ttgttagtat ccctttccaa gcgtatgtcc
     4441 caatccctac aggcatccag aagttagcag aggtgatcga agagaacata tgatggaata
     4501 ggagtggatg ataatgaaac agaaggaaaa attgctgttg agatatttga ttgatcacaa
     4561 gaaggagtat gtcaccagtc aacgtttagc ttctgaattg ttattatctg atcggacaat
     4621 ccgtaattac ttgcagcgga tcaaagagtt agtagaaaaa aacggaggta agatcattgc
     4681 taaaccaggt tatggctacc aactgcatat tttacaaaga ctgacatttg acttgttttt
     4741 gagtcggcaa gaaatagtac ctggatatgc tagagaaccg caaggatttt atgagtccga
     4801 agatcgacaa aagtatatat tgaaccaatt gcttttggaa gatgccattc tttttatgga
     4861 tgatttggca gaagagcttt atatcagtcg ttccagtttg acaaaggaca tgcaagaaat
     4921 caaggagcga ttgattccat attcactaaa aatcgtttct aagcatggtc aagggacatg
     4981 gatcgaaggg gaagaaagaa atcgacggca ttttatcatg gatacttttt ttggaaacca
     5041 ttatggaaat tcattgaaag aatatttagg gcaaagccgt tttttcccag acatcagttt
     5101 tgaagagcta gtcattatca ttctcgatga aacaagagaa gctaagttaa aagtttcgga
     5161 ttttatcata cagaatctga ctttacactt ggctttgggg atcaaacggc tccgagaagg
     5221 gtttgaaatc aaggaattag gcatcgaaca agaagtttct aagcgagtag agtatcaagt
     5281 ggcgcagcgg atcgttcaac gtattgaggt aatcgcaggt gtatcgtttc ccgaagaaga
     5341 aattgcttat ttgacattac atctcatggc taagtcgaat cacaaagaaa atcaaaatga
     5401 tcaggaacta tcaaaggaat tagcgaaagt tctgaaaaga ttgtctcagg aatttcattt
     5461 ttcattagta gaggatctcc agcttttcaa tggactactc gatcatttgt caccgatgat
     5521 gattcgattg agccgaggta ttcgcattga gaacccattg actgaggaga taaagaaaga
     5581 aaatcccaaa gtctttgacg cagtaaaaag gcatttcagc aacatgccgg ctttgaaaaa
     5641 ctataccatc aatgaagatg aatgggccta tttggctttg catctcatgg cggcattgga
     5701 aaaagaaaga gctgcgcata aactacatgc attgattatc tgtgcaacgg gttacggcag
     5761 tgcccaacta ttaaagaatc gcgtagtcag tgaattcggt aaaaatatta cagtggtaag
     5821 tgtaaaaggc tattatgaga tcaatgaaac aactttaaaa ggaatcgacc tgattatttc
     5881 ttctattgat ctttcgacaa tgtttttcaa aattccagtt ttacatgtca gtattttttt
     5941 gaatgaagat gatgttcgta aaatacgaaa ggtagttgga gaatctatac cctatcattc
     6001 ctcagataga ccgataccag cattttctct acagcaaaaa aagcagattt acaccgaaca
     6061 gttgtctgaa aaatttttca agacttatac gtacgaaccg aaaaaggaag aaattcttca
     6121 tgatttgctg caaaagctga gcataaatga agaagaagga tatataagag aaatgcgaaa
     6181 gcagatccag caacgtgaaa atatgggaca aatcatattc agtgatacag tggcagtccc
     6241 acatcccgct gttccagttg gaatcgaaac aaaggtagcc gttgcattga ttccttctgg
     6301 gatgcagtgg gatcaatatc aagaaattca ttttgttttt ttagtttcgc cttcttatgt
     6361 cgaaaatgaa ggaattacgg tcgtgactaa agcaatcgtt aggttagttg accagctaaa
     6421 agtacagcag gaaatcttag ctgaacctat ttttgagaat ttcagcagta aatttatcaa
     6481 actgatcgaa taaggaggaa aacagatggc tgaaaaaata aacagtgaaa cactacaagt
     6541 gacttctttt gatatcattt tccatagcgg aaatgcacgg acactgatac atgaagcatt
     6601 tcgctcgatg cgtaaagaga tgtttgaaga agtagaagag aagttgcgac aagcaaatga
     6661 agagattctt catgcgcatc agtcacaaac tcaacttctg aaagaatatg ccagcgggac
     6721 aaaaatagaa atggaaatta tcatggtcca tgcacaagat catctgatga cgacgatgac
     6781 attattggaa gttgcacagg aaatgaatta tttgtatcaa aaaaataaat agaaaggaag
     6841 gatcattgtg caggatacag taaaaaggaa aatcgaatta gaaaaaaagc aacttagttt
     6901 gaaaaacatg tatttcaatc gttatctaat cgtacgttac ctgactgcct ttttcttttt
     6961 catgaacatg caatggatga ttctgcttct atcagctaaa agtttaggaa gtagtttacc
     7021 gatgattctg attttagcta ttttgcctgc agtaggagaa caagtaaagc tttatcgaaa
     7081 acaccaaacg aacgttccat ggacaaagtg gtattttttg ttccagagtg tctttaattt
     7141 attagtgatt ccagtgttgt ttacctcagg atttacattc ttgtatcctt ttatggcaaa
     7201 taacaatgga ggacagttat ttgttttcat tcttgttgta agcggtattt ttgccagtgc
     7261 atttatacag tatcgtttaa agaaaatttc actcaaccaa gaccaacagt atatccgtat
     7321 taaacaatat gaaaaagcgt tatatttagg aaaggagaat aactaatgtt tgattttttg
     7381 cagaaatacc tgatgggacc tatggggaaa attgcgcaac ttaaaatcgt tcgggctgtc
     7441 atggcagcgg ggatggcttc tattcctttc acgattgtag gctcgatgtt tttggtttta
     7501 aatattttgc cattaccgtt cccattttta gaaggatttt ttaatgcaac ttttttcaaa
     7561 gttagtgact tgtacatgat cgtcaataca atgacgatgg ggattttatc tatttacttt
     7621 gccattgtgt ttgcttatga attaacgtca attgaacgag atgaacaaaa attaaatgtt
     7681 aatccgctca cgggggcatt actatctgtt tttgcattct ttatgtgtat ccctgaattg
     7741 atcatgactg atggtaaaat cagcttgatt gcttctatca atgagacaga aactgtagta
     7801 aatggtatta ggatgggctc ttttgtcgaa cgtttaggaa cttcaggaat ctttacagct
     7861 attattatgt cgattattgc ggtcgaattg tattgtatgt gtgtcaagag aaattgggtc
     7921 ataaaaatgc cagatgttgt tccgccagga gtttctcgtt ctttcactgc tctgatccca
     7981 acatttgtca ttgcgtttgt ggtgatgatc atcaatggat tattagttgc actaggaacg
     8041 gatatcttca aaatgattgc tatcccattt gggtttgtta cgcagttgac gaatacatgg
     8101 cttggtgtca tggtgattta tttcttgatc catgcattgt ggatcgtagg gatccatgga
     8161 gcgaatatca tcacttcttt cttgaccccg atcgtattag ctaatatggc agctaacgca
     8221 caaggcgcaa attatccgtt ggcaggagaa ttcaacaatt cttatgtaac cgttggtgga
     8281 tcaggtgcga ctttaggatt gatcatcttt attgcattcc tcgcgaaatc tgatcagctg
     8341 aaagtgttag gaaaagcctc acttgtacca ggattattca atatcaatga accaatcatt
     8401 ttcgggatgc caatcgtcta caatccctat ttagcggttc cgtttttctt agctccgatg
     8461 gcttctgcta cactcgctta ttttgccatt cggctggaaa tcgtcaaacc gatgcttgca
     8521 cagatgccgt ggccttcacc agtcgggatc ggagcatttg ttggtagtgg tggtgattgg
     8581 aaagctgctg tgttagcagt catttgtgca atcatcgcat tcattatctg gttcccattt
     8641 atcaaattct atgataataa attattaaat gaagaaagag aaaaggcagc agaattagct
     8701 agtgaaaagt taaaatgatt tctaagaaaa aaggaactgt gacaaaaaat cacagtccct
     8761 ttttttctta gaaatgggaa accactagta tataaatacc attattcttc aatcaaccgc
     8821 ttcacgattg cataatcccc ttcataaggt tcatctacac cattgatttt ttgataggga
     8881 tccacgcctt ttcccgcaat aacgactgaa tcttgcggag cgctttctgc taaagcgcgt
     8941 cgaatcgctt caggacgatc catttcataa tgaacgatca aatctggatt agtaattgct
     9001 tcattgattt cttccgcgat ttttttcgga tcttcaaatg ctgggtcatc tgccgtcaaa
     9061 aaggcgatgt cagctgtttc tgatagcact tttccaaaat catgtcgacg ggagattgct
     9121 ttgtttcctg ggctgcctaa tacaacaatt acacggccat ctggatgttc atttttggca
     9181 aattccagca atgttttcaa gcttagataa ttatgtgcat agtccacata gacatgcgct
     9241 ccattggatt ggatcaattg atccattcgg cctggtacac gagcttcttt taatccgtct
     9301 tgtgcatctt gactggaggc acctgttagt gctgcggcaa tgatcgcact ggctgcattt
     9361 tcttggttga atcctccagc cagcaaaata tcataagcag tatcgcagat agctaagcga
     9421 tcttcatgag aagataaagt aaaaccgtga gtacctttat ctgaacggat cacttgataa
     9481 ttgctttctt ccgaacgacc ataagtgatc gttggaatct ggaatagatc ggctgtttcc
     9541 ctaagtaaat caaaatagtc actttcatga tttaaaacaa ctgtttttga attcaagatc
     9601 aattgtcgct tgcaatagaa atagtcatca aatgtcggat gttcgatcgg actgatatgg
     9661 tcaggcgaga tattcaagaa gattccgaca tctaaagtca agccgtatac gcgttgtgtt
     9721 ttgtaagctt gtgaagaaac ttccatcact agatgagtca taccattttc aactgcttct
     9781 gacatcatac gatataaatc taatgactca ggcgttgtca aatgggattt gaaatatgtt
     9841 ttgccatcta acgttgtatt catagtagaa agcaatgctg tttttttatt tgttgtgtga
     9901 tccaagattg cttttgtaaa gtacgcagca gttgttttac cttttgttcc agtgaatccg
     9961 atcactttca atttattttg cggatagtcg taaaaagcca tagcaagaag cgccattgct
    10021 ttacggatat ccgttacgat gattcctact gctgaatttt cgtatggttg ttctgataca
    10081 taatattgaa taccctgttc gattgctgaa tccaaatatt cttctttgaa attcattcct
    10141 ttacagaaaa acaaagtaga agcatcagct tttcttgaat cataacttaa ctgaagaaat
    10201 tcagcttttt ctggtacatc aagatgccag cctcttgttg aaacaaattc ttttaataga
    10261 ttttctttta atagtaaatg acggatgtct tctaaagaga agctcattga ttgatccctc
    10321 gctttattca atttcttggt ttattatagc acactctttt taatatatcg tttgtttccg
    10381 ctatgacaaa atccagcttt attcaaattt tcttgacaca ggggacaagc ggatgagtat
    10441 tttcataaac aatgaactga ctgtaataga taggagaacg aatagtgtct ccaacaatcc
    10501 aaaaacataa taattcaaaa agacgataag actattggta agtaaaagtc cttgcggcat
    10561 tggaagccga aaaaatttgt ggaaaataat cggcggtatc gttataccgc catttgaggc
    10621 cccgactgtg tacaatgtgt aaaccccaag actgaacaaa agacttcctg aaatcagtga
    10681 aaagaaatgt gaggagatca tttccacttt tggaacaatc tctaaaacaa caggaaaaag
    10741 cagacttccc gctacagtct taaaaaatac tgattttcct aataggacag cggctagaag
    10801 cagcatcata agattgatgc tccaaacagt aactgagcgt tggatagaaa aggccgtttc
    10861 aactaaatgt ccgatggcgc ccactccggc ggaagctata tcgtgaggga gcaagaacca
    10921 attcaatcca actgtgataa agaaaagacc taaaaatata acgggtagtt tttttatttc
    10981 ttgtgtcatt gatatctcct ataaatgtta gccataaaag gctgtatatt tctttacttt
    11041 accaaaaaaa cgaaaccgaa ttcaaaaaaa caattgttct tataaaagtt atataaaatc
    11101 gattgccagt ttctgtttta ccaattaaaa tgatagtaat gttttgtaga aagttggaat
    11161 ctgaccgatg gataatcaaa gaaaaccagc attagatcaa ttaacaccgc aagaaaaaat
    11221 ggcacgaggc tctgcttgga tgaccgccag taatatcatt tcaagattgc taggcgctgt
    11281 ctatatcatt ccctggtatg cttggatggg cgaaaatgct aaggctgcca atggattatt
    11341 caatatggga tacaatatat atgcattgtt tctactgatc tctacagcag gaatacctgc
    11401 tgcgattgca aaacaaactg cacgctataa ttcattgaat gaatatggca cttcacgacg
    11461 attatttatc cgtgcattac agatgatggg cgggctaggc ctgttgtttg ctttgttcat
    11521 gtacatcgcc tctccttggc tagctcatgc ttctggtggc ggagaagaac tgattccaac
    11581 gatgcgttct ttgagtatcg ctgtattggt ttttccttgt atgagtgtga tacgagggta
    11641 ttttcaaggc aatcaagaaa tgatgcctta tgcgctgtcg cagattgtag aacaggtggc
    11701 acgtattttt tatatgctat tggctacatt catcatcatg aaagtcatgg atggtgacta
    11761 tgtgacggcg gtcacgcaat caactttcgc ggcatttatc ggtatgctag caagtattct
    11821 tgtccttttg tattttttga agaaacatca agcgtataca tcggcgttta ttcactacag
    11881 cgaaaacaaa ataaatattg caacgaagga attgttgtta gataccgtta aagaagcgat
    11941 tccgtttatt attgtgggtt caggggtaac gatcttcaaa ttagtcgatc agttcacttt
    12001 tatgaagatc atgtctgcat ccaccgagta ttccaatgca cagttactgg atcttttttc
    12061 gatttttagt gccaatccag ataagttgac gatggtagtt attgcattgg caacatcgat
    12121 cgcgtctact gggttaccgt tgattacaga agctgttacg ttgaaagatc gagtaggtct
    12181 ggcaaaattg acaagcggaa atttacagtt gttttcattt tttatgtttc cggcgacatt
    12241 tggtgttatg ttacttgcct atccgctaaa tacattattt tatacaccgg attctctagg
    12301 aagtaatgta ctgatccaag caagttttgt aggactcttc ctaggactat atatgctagt
    12361 ttccaacatg ctgcaaggaa tgtttgaaaa caaggcggca attaattatt tggttattgg
    12421 gtttctagtg aaactccttc ttcaataccc agccattcgt ttatttgaag tatacggccc
    12481 tttattggcg acaatgatcg gatttgctgt gtcttgtacc ttgatcttaa agagaatcca
    12541 tgaagtagcc cgctttaaac caagttttgt ctggcgcagg acattgctga tctttattct
    12601 taccttgatt atgcttttgg cttcgtggat cacaaagatg atttttggtg cttttttaaa
    12661 cgaagatagc aaattccagt ctctattgtt gattcttgta gtagcaggta taggggcttt
    12721 agtgtatggt tatttagctt tgaaaatccg cttggcagaa agactactag gaccaggaat
    12781 agcgcgttta cgacataaat taaaaataaa ataagtgaat cagaagagct agattgatgc
    12841 tcttttgaaa tgggggaaac gcatgcgttt agataagttt ttagcagata tgggacttgg
    12901 cagccgcaaa gaagtcaaag gattgatcaa aaaaggaacg atcgaagtca atggacaagt
    12961 ggtgaaaagc gataaagcgc aagtaaatga attcgaagat gcggttactt atttaggaga
    13021 aatcattacg tatcaaaaag atttttacta cattttgcat aaacctgcag gagtcatttc
    13081 tgctactcag gataatcatg atcaaaccgt aatcgattta ttggaagacc aggattatcg
    13141 ggaagatctt tttccagtcg gccgattaga taaagacaca gagggcctct tgattttaac
    13201 gaatgacggg gtgtttgccc atcagttact atcacctaaa aaacatgtgg agaaagaata
    13261 tctagctgag gttagcggta taatgacaga agaagatatc cgtttatttg cagaaggact
    13321 gattattgat ggcgatgagc agacattgcc ggcaaaactc caaattgatc aagtagatca
    13381 agagacagaa acttcaatta tccgtctgat tttgcatgaa ggaaagtttc accaagttaa
    13441 gcgaatggtc aaagcagtag gaaaagaagt cttatattta aaaagaatcc gtatgggaaa
    13501 attttggctg cctaaagatt tgaataaagg gcaatatcga cccatgacca aagaagagct
    13561 agaacaagtg agagaaaaat aattgaatgg atagctaaaa gaataagcag agtagttgta
    13621 gcgccatctg aaaagcagac ttgtccgtct gactttcgga tggctttttt gtgaaatttc
    13681 ggcttatttc cgtagaagct acttctggaa caccgtttgt tcggaataag ggactgtgac
    13741 aagacttttg ttacagtccc cattgttcct tgtgactcag agttagatag taaggacacg
    13801 atctccctaa tactcaggcc aaaacgctga attcacaacc tctgataaac ggtgttcaat
    13861 cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttcccgttt
    13921 ttttctctaa tactcaggcc aaaacgctga tttcacaacc tctcctatac tttgtttcat
    13981 tttccctata ctttgtttat ttattctcat tattgaaagc gctataatta cgttgcgaat
    14041 gaatcgaaag gggtttttag atgaaaaaac gaaaggggtt caatcatttt tttatgaatc
    14101 tatggcgtta taaagcactt gtcctgatgg ctgtaccggg tatgctttgg atgatttttt
    14161 tcttttacat acctgtaatg gcgaatgtcg tagcattcaa aaattttcat atttcagcag
    14221 atgggttctt ggctagcttg agggaaagtc cttgggtggg ttgggaaaat ttcaaatttt
    14281 tattttcttc ggatcaagca tttctcatca ctaaaaatac aattttatat aatgtagcat
    14341 ttatcctttt gaatctgctg atctcagtcg tatttgcaat cattatgagc gagttgagaa
    14401 ataaacgact cgttaaagtt tatcaaacga tgtccttact accttatttt ctatcttggg
    14461 tcattatcag ttactttgtt tacgcttttt taagtcctga caaaggaata ttcaatcagt
    14521 ggatcactgg acatggagga gaagctgtca attggtataa tgaaccgaaa tattggccgt
    14581 ttatcttggt gttcattggt acatggaaag ggattggtta taacagcatt atttactttg
    14641 catctgttat gggaatcgat ccgacttatt atgaagctgc tatggttgat ggagctagca
    14701 aatggcagca gatcaaacat gtgacgatac cacaacttat tccactaatg acgattttaa
    14761 cgattttagc cgtaggaaac attttccgag ctgattttgg cctgttttat aacattccta
    14821 ggaattccgg tgcattatat gatgtgactc aagttttgga tacgtatatc tacaatggcc
    14881 tgacatccac aggagatttt ggtatgacgg ctgctgcagg actttaccaa tcagttgtcg
    14941 gctttgtctt gttgatgatc acaaatacga tcgcccgacg ttttgatagc gattctgcat
    15001 tattctaagg aggaaaaaaa tgaaaaaaaa gaaagtggag agagtccaag tccgctcttt
    15061 caataagacg gctaattttt tctctaatat aatgattgca ctctttgcta tttcctgtat
    15121 tttgccattt atatttgtta tcgccatctc ttttaccgat gagtccatat tgtcgactca
    15181 cgggtacagt ttttggccag aaaagttcag tatatttggt tatacgtact tgttcgacca
    15241 gatgcaggat aaaattttcc aagcgctttt cgtcactgta cttgttactg ttttaggtac
    15301 actgatcaat agcacggcta cttctttata tgcttatgcg atttcacggt caaactttcc
    15361 attccgtcgt ttttttaccg ttttttgttt gattacgatg ttgttctcac cagggatggt
    15421 ggccaattat cttgttatga caaatcttct tcagttaaaa gatacgattt gggcattgat
    15481 cttgccaatg gctgtcagtc ccttcaatat tattgtgatg cgcacattct ttaaacgttc
    15541 ggtctctgat tcgattattg aatcggcacg gatcgatgga gctagcgaac tgcgaatatt
    15601 tgttcagatc gttttaccgt tagctgttcc aggtattgcg acaatcagtt tatttgccgc
    15661 attgggttac tggaatgact ggttcaatgc tttactatac atccaagatg ataagctagt
    15721 tccactacag tatcttttga tgaaaatcca aagtaatatt caatatctga cacaaaattc
    15781 aggagcagga agccaattga caggcggatt agcctcagtt ccaggagaat ctgccagaat
    15841 ggcgatcgta gtcatctcga cgttgcctat tgcgatcagc tatccatttt tccagaagca
    15901 ttttgtcaaa ggtctaacaa tcggcggagt aaaagaataa agaaacaaaa atataaatca
    15961 taaaattagg aggaagaaaa atgaagttat ggaagaaact agcctttaca ggtgtttcag
    16021 cgttgttgtt gggaacatta gcagcatgcg gaggttctgg ttctaaagac caggcagaag
    16081 ctagcaattc ggacactttg cagatgtatc aaatcggaga taaaccagat aatttcgatc
    16141 aattgatgga agtagcaaac aaacgaatcg aaaaagagat cggtgtaaaa gtcaatatca
    16201 attacattgg ctggggcgat tacgaaaaga aaatgaacgt catcatttct tccggggaaa
    16261 attatgatat cgcctttgcg aacaactatg tttccaatgc ccaaaaagga gcctttgctg
    16321 atttgactga attagcgcct aagtacgctg aaaaagctta taatgattta gatgaagcat
    16381 atattaaagg aaatctggtc aatggaaaat tatatgcttt cccagtcaat ggaaatgtct
    16441 ttgctcaaca agtacttact tttaacaaag ctttactaga taaatacgat ctttcaattg
    16501 atggaatcca atcttacgct gatgcagaaa aagtcttgaa ggaattccat gaaaaagaac
    16561 caaacacggc agcttttgcc attggacaag gcttcaaagt ccaagggaac tttgattatc
    16621 ctttagggaa tacgttacca tttgctattg atctggatgg agatgattcg aaaatcatca
    16681 accaatacga taatgaaacg tatatcggac tgttacgtac aatgcatagc tggtacaaac
    16741 aaggactcat tccaagtgac gctgcaacga gcaataagga ttatccacta gaaggaaata
    16801 cttggctaat gcgtggagaa acacaaggac cgtatgatta tggcgataca attttgacaa
    16861 atgcagcaaa acaagaactt gtctcaaaag caattactgt tccactaaaa tctacagctc
    16921 aagcacaaat ggcaaatttt gtcgtatcaa atacttcaaa aaataaagaa aaatcagtag
    16981 aacttctagg gctgcttaac agtgatccag aattattgaa tgggcttgtt tgggggatcg
    17041 agggagaagc atgggaaaaa gtcgatggtt cagatgataa aatcaaattg ctagatggtt
    17101 atcagccaaa tacacatatg tctgcttgga atacaggaaa caacaagatt ctatatacac
    17161 aagagtctat tacggatgac atgattgcca aacgtgatca atcgattgct gatgcagaaa
    17221 cttcaccgat tttaggattc agcttcaaca ctgacagtgt caaaacagaa ttaagtaata
    17281 tttctaatgt catgaatcaa tatttagatg gattgaatac agggactgtt gatccagatg
    17341 aaacattacc gaaattgaaa gacgctttgg acaaggctgg ctatgataag gtattgaaag
    17401 aaatgcaaaa acaatatgat gagttccgac aagaataata atgaataaag acaaaggggt
    17461 tgtgacaaaa gacgtgtcac aacctcttgt tccgaataaa cggtgttcaa aagccagttt
    17521 ctcggtatta agctaaatat atgtaaaaat gtggaaagcc atattcacaa aatttttctt
    17581 actactcgaa actaagcggc tatttcacaa actcttggta aacggtgttc aatcagctga
    17641 ccctcggtat tcgctcacaa aacgacaaaa tatgaggaac tatttttccg tttgtttctc
    17701 taataatcag gtcaaggtgc tgattgccca accttttttt gtataatgag aaagtataaa
    17761 cttaatgttt ggaggagaag atatgttagg gaaggttttg gaagagttat ttatccggat
    17821 ctgggtcatt ataaaactaa ccttgtattt ctggatatat acatttgccg gcgggatcat
    17881 ctttggatta ggtgcggcat ggaaaacagt caatgaacta ttttatttgt acggattcga
    17941 gtataaagaa atcacaataa aaagaggctg gaatatatat aaaagaaatt ttttaagagg
    18001 caatctgcta tttagtttat ttttatctgg tactgcttta ttgagttaca atttatattt
    18061 atctgttcaa atcaaagggc tcattttttt agctattgat tttattttac tttttgcctt
    18121 gctctgccta tttgctactt atcaatacag tatgatttta gatagtgaat atacgatctc
    18181 tgttcctaat ctgctaaagc taagttttat ttcggttttt tccagctttt cgtcttttct
    18241 aaaaataatt attggtagcg gtataatttt aggcgtgact tggcaattca aaggattgat
    18301 cctgtttggt gtcattggtc ttttgacggt ttggaatggt acgatgacaa cacattggcg
    18361 tgaagaatta gataaacagt tggaaagtta tgaataagtt tcggattcta ttttcaaaag
    18421 gaaccttact aaatcgtctg ttgcggaaat acgcggttat catgatcggt gtaacgatga
    18481 ctgctaccgt tatttttagt atccatactt gggaacaaaa tcaaaaacag gcagaaaaca
    18541 tgacaagtga tgccgttcaa agcacaagca gaatgctgaa tgataaaaca accttgagta
    18601 ggatcatcaa aaaccagctt gtagggaatt ccgaaaagat cgaaaatgtg acgacctact
    18661 taacaaaacc catagatcag tatctgatgt atgtatacga acagcaaaac agtaccgatg
    18721 aattagtttc tttcccaaat caaatcaaag atctctacgc aaattatgaa gaactaagtg
    18781 cgatatatat cgtgttgaat caactaccgg agtattatga atcttctaga gaaaacaaag
    18841 gtggggaaat aaaaaagggg acaccaaaat tactagacgc cttttatatc aagttgccta
    18901 tcacacaagg aggagctgag tcaggaagta tttttattgg ttttcaaaag aaagaactag
    18961 atatgatttt agaaaatcta acgtcattca atggactctc tttatacatg atttctggaa
    19021 cgacaaatcg actatacaca ttccatgata ataaaatagc taaagaaacg tttgaaagac
    19081 aagaaatgat catcacagag agtctgaaga aaaattcaca attgccgatt gacgatctgg
    19141 aaaaaaacaa ttttgttcaa caccaagaac tgtctgatga ttatcgtata ttagctgtac
    19201 tagataaaaa aagtgtaaaa atcgaaacgg gaaaaaatct tgccccgctg atcattggta
    19261 tcattatttt agacaatata ctgcttatct ttctttataa aacattcaaa cgatattcac
    19321 agcaagtatc tatcgtcatg caggccatgg atcaaacagc agcagggaat ttagagacgc
    19381 ggatcgacat aacaaacacg gaatatgaac tgaaggaact ttcaatcggt atcaacgaaa
    19441 tgttggatag catcaatcag tttgtagaag acatctacaa attagaaatc aaacagcaag
    19501 acgcacatat gcgggcgctt caagcacaga tcaatccaca ctttttatat aatacattag
    19561 aatatatccg aatgtatgca atcagcgaag gaagtgaaga attagcagat gttgtgtatg
    19621 cgttttccgc tttgcttcga aacaatacga atcaagagaa aacgatcact ttaaaagaag
    19681 aactggactt ttgcgaaaaa tatgtctatt tgtatcaaat gcgttatcct aatcgagtag
    19741 cctatcattt tatgatcgat cctgatttgg aaaagatcga agtacctaaa tttgtgatcc
    19801 agccattagt tgaaaattat tttaaacacg gtattgattt tactcgattc gataatgcgc
    19861 taagtgtcaa agtcttgcag gaaggcaaac gggtaaggat catcatcaaa gataatggaa
    19921 aagggatgac tgaaaaacgg ctaaaacaga ttgaagaaaa actgtctcat cctaaagtgg
    19981 aacttcacgg ttctatcggt ctgcaaaacg tgaatgaacg tttacgtgcc agcttcggca
    20041 gcagttatta tatgtcgctt gagaataatg agacaggtgg actgaccgtt tctattacat
    20101 tcaaggaggg atgagcgtgt atcaagtgtt attggtagat gatgaataca tgattttgaa
    20161 tgggttaaaa aagataattg attggcaaag tttaggcttt caaatagtag caacagcaga
    20221 gaatgccaaa gaaggactag ctgtacttga acagcgacag attgatttag tagtaacgga
    20281 tgtgacaatg ccagaaataa atggactgga atttattgaa gcggctcaaa aagaacgtca
    20341 taattttgaa tttatgattt tatcaggata ccaagaattc gattatttga aaggaggtat
    20401 gcaattaggc gccgtgaatt atttgatgaa acccgtgaat aagttcgaat tggttgaaag
    20461 cctcaagaaa atcaaaacac ggttagatca gcaaaatgaa caaaaaaatc aacaagaaat
    20521 ctatcaagaa ctgttattca gtcaatggtt gaatgaagaa ttggatgaaa taagtgaaga
    20581 agaaataata ggacaaataa gtgagaagca gcataggata gtattgattc aactggcaag
    20641 aactcatgat actcttatca atagatggtt gcaagaacgg caacaaatct tttactatca
    20701 acgaaattac ggagaccgtg tattgatcac gttgttgttg gaggcagatg aagttgatgg
    20761 cttctgttgt tttgttcaag aaaactttcc tgatcaggaa tggctgatca gtatcgggga
    20821 agagacagat tctttggaca aaatccctga aacattccaa caagcgaaag acaatctaca
    20881 gttgcatcaa ttttatggag ataaagacga gcatgttttt tacgcagtac aagcagaaac
    20941 aaaagatcaa accatcgatt tttcaagttt cagacgcgtg ctgcaaaata aacaattgga
    21001 agtcgctcaa aaaatgatca ctgatttttt tgaacagttt cagttagcag ccatgatgcc
    21061 ggaagatata cgatattcca gttttttact gtttatggag attcaacggg aattgattga
    21121 tctggaagat gaggaatatt tacaaggaat cgaaaaaatc catcaggcaa aaacggtgca
    21181 agaacttcag cagctacttc tgtcattcat caaaaagcat cagagacaga aaaagtacag
    21241 tgacaatgtg gaaaaagtaa tcgtaatcct gcaccagcat tatcaagaac cacttacact
    21301 caaagaagtg tcagaaaacc tgcatttaaa tgtcatgtat ctaggacagc tgttcaaaaa
    21361 agaaacgaaa aaaagttttt cagcttattt gaaccattta cgtatggaaa aagcgaaaca
    21421 actgcttttg cacagtaatc aaaacatcaa tgagatagca agtgagattg gctacaacaa
    21481 caccacgtat ttctctaaat tattcaagaa aatcgtcggc agatccccta aagagtaccg
    21541 agaaaacagt ggagaatcat aaaaaaaaaa gccaatgtaa tgaaataact tataaaaaat
    21601 tgtctagaaa tctaattttg ggctgttctt attacttgag gcaaggcagc tttttcatga
    21661 cctctcgata aacgg
//
LOCUS       DAEGIQ010000043        21099 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000043 DAEGIQ010000000
VERSION     DAEGIQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.043"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..767
                     /locus_tag="KMT45_001430"
     CDS             <1..767
                     /locus_tag="KMT45_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004900.1"
                     /translation="IKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWR
                     LNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTL
                     IAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELD
                     NSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKL
                     AIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(912..1652)
                     /gene="truA"
                     /locus_tag="KMT45_001431"
     CDS             complement(912..1652)
                     /gene="truA"
                     /locus_tag="KMT45_001431"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBE7004901.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1843..2478)
                     /locus_tag="KMT45_001432"
     CDS             complement(1843..2478)
                     /locus_tag="KMT45_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBE7004902.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(2625..3866)
                     /gene="sstT"
                     /locus_tag="KMT45_001433"
     CDS             complement(2625..3866)
                     /gene="sstT"
                     /locus_tag="KMT45_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBE7004903.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4064..5062
                     /locus_tag="KMT45_001434"
     CDS             4064..5062
                     /locus_tag="KMT45_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004904.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5075..7093
                     /locus_tag="KMT45_001435"
     CDS             5075..7093
                     /locus_tag="KMT45_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBE7004905.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7153..7869)
                     /locus_tag="KMT45_001436"
     CDS             complement(7153..7869)
                     /locus_tag="KMT45_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBE7004906.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8096..8638)
                     /locus_tag="KMT45_001437"
     CDS             complement(8096..8638)
                     /locus_tag="KMT45_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE7004907.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(8643..8870)
                     /locus_tag="KMT45_001438"
     CDS             complement(8643..8870)
                     /locus_tag="KMT45_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBE7004908.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8949..10829)
                     /locus_tag="KMT45_001439"
     CDS             complement(8949..10829)
                     /locus_tag="KMT45_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBE7004909.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11018..11632)
                     /locus_tag="KMT45_001440"
     CDS             complement(11018..11632)
                     /locus_tag="KMT45_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7004910.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11774..12334
                     /locus_tag="KMT45_001441"
     CDS             11774..12334
                     /locus_tag="KMT45_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE7004911.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(12654..12953)
                     /locus_tag="KMT45_001442"
                     /pseudo
     CDS             complement(12654..12953)
                     /locus_tag="KMT45_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(13207..14842)
                     /locus_tag="KMT45_001443"
                     /pseudo
     CDS             complement(13207..14842)
                     /locus_tag="KMT45_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15397..15960)
                     /locus_tag="KMT45_001444"
     CDS             complement(15397..15960)
                     /locus_tag="KMT45_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBE7004912.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(15975..17018)
                     /locus_tag="KMT45_001445"
     CDS             complement(15975..17018)
                     /locus_tag="KMT45_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004913.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(17151..17555)
                     /locus_tag="KMT45_001446"
     CDS             complement(17151..17555)
                     /locus_tag="KMT45_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBE7004914.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(17788..18423)
                     /locus_tag="KMT45_001447"
     CDS             complement(17788..18423)
                     /locus_tag="KMT45_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBE7004915.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(18689..19741)
                     /locus_tag="KMT45_001448"
     CDS             complement(18689..19741)
                     /locus_tag="KMT45_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7004916.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(19754..20692)
                     /locus_tag="KMT45_001449"
     CDS             complement(19754..20692)
                     /locus_tag="KMT45_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBE7004917.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         6691 a         4184 c         3337 g         6887 t
ORIGIN      
        1 ttataaaaaa attaactgct gatgatttat ttattttctt tcttggtgta tttgttcttt
       61 tttgttcaac caatttattt ccttggcaac tttttaacca tacaccagtt aagagtctac
      121 aatttgtctg gcggttaaat ggtttttctt ctttatttat agcctatacg atgagtatag
      181 tgatctatta tattttttct actaaaaata attcatggaa aataatggtc tttacgtttc
      241 ttgcactaat attgcatctc tcaggagtaa gcaattctat ccatgctaac aaagattcat
      301 taacacttat agcacctgaa gacgctgttg ctatagctga aaattacaat catactgatt
      361 atgcaaacaa agagtcaatc tatcatcctg atatgattaa taatgatcaa tatttactag
      421 gtaaccagga aataaatcca acaactcatt ttatgagtaa caaacttaca attgaattag
      481 ataattcgaa taataaaaat actgtcttga cgacacctat ttatcgttat aaagggcaag
      541 tggcttctat taatggcaaa ctagtacaaa caaaattatc taagtttggc acaactgaat
      601 taactattcc tccaggtata aataaagtcg ttattactta tcagtacaca aaactagcaa
      661 ttgcttcaag atatttatct atcgttactt tgattttatt tttactttat cgatttactt
      721 tttctaaaca aaaacaaccg agaagagaag tacaacattc tcactagatt tttatttaag
      781 aaagactttt tagctgtaca cataaacagg ctaaaaagtc tttttttata tttctttaat
      841 ttgcttcata cattatttaa catcgatatt tatactgtga aacatgcgtt tcatccatta
      901 tcaaagtatt tttatcgata ataaacttca tccaaaaaca gaccttgtgc agggacggtt
      961 tctcctgcat gttgccgcac tttgctttca aatatttcat cgattgtttc tatcggcatg
     1021 gcacctgtgc cgatttctag taatgtcccc atgataatcc gcaccatttt atataagaat
     1081 ccgtctccga caaaagtaaa atgaagcatt tctccctttc gttcgataga taattcatca
     1141 atcgtacgca ctgtggattt ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt
     1201 gtacccacta atttttcgca agctgcattc agcttgtcca tatccaactc ttgagggaaa
     1261 taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt tgttccaaac ataatagctg
     1321 tattttttgc ccaccgcatt atatcttgaa tggaagcgct caggcatttc ttcgattctt
     1381 ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt cgatcatttc tgccagtcca
     1441 atttctgtgt ttgttttaaa atttgctact tgtcctcgcg catgggtgcc agcatctgtt
     1501 cttcctgatc caataatttc tatctcttca tttgtcatct gatgcaatac tttttcgatt
     1561 tttccttgaa tggttttatc gtcatctcca agtctttgcc atcctgaata tctttttccg
     1621 tcatattcga ttgtcaactt gatgtttctc atattttagt ggctcctctg tcttccattt
     1681 ggtcttctct ctaccttttt aagattgtag cacaaaatat agctccttgc ttctctaaga
     1741 cagaagctat acaagaaaac tgtggaaata tatatttcag aaaaacaaaa aacagccacc
     1801 ggatgaagta tccgctgact gttcaatttg acggctcaag ttttactgtt tgagatgcaa
     1861 gatactcaac caggcaatgg gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc
     1921 tattttattc agtaatggcc aagaatttat agaaggaatt cttgccagtc cataaatcgc
     1981 cgcaaaaagc aaactgatca cagacagtcc aaggtataag tggcttacgt ccatttgccc
     2041 attttttcga tagatcaaga aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa
     2101 acctgatccg atattatgga cccatgtcga gacattccac gaagattcat gggtattgat
     2161 actgaacaaa cctgtaaaga tacaatcacc tataccatag aacgcgatgg ctgctgtaac
     2221 tagggtagct aacacacgag atgtactaac aaatgcagca tacaatgcag gcaatgacag
     2281 gatgaaaaag atccctgaaa ccacagacca aataagaaac gcttttctta caggactgga
     2341 aacctctcca aatacactga tcacgctctt catttgatct aattcaggat aatacaatcc
     2401 taatacgtac ggagtcaaaa aatcactcag cacgcctaat aataaaaaga aaaaaccaaa
     2461 tttacgtaaa aaatccatga tacgcctcca ataacaatta cctatgcttt taattgttat
     2521 tataaacgac tttatcttga tttaacagaa acctctattt acacgaacat ctatataact
     2581 cattaaaaac tcgaatcatt ttaaaaatac taaatatcta acgttcaacg ttcacttaat
     2641 ccatctaaac aatctttcaa tttgattttt tccccacttc ttttacgcgc gcctagctct
     2701 cctattgcag taaacaacag atcacttgac gaatttaagg cagtttccat tgaatcctgg
     2761 ataacaccga taataaatcc aataccgacg atttgcatcg cgacatcatt agagatacca
     2821 aacaaactac atgctaatgg aatcaataac agtgaaccgc ccgcaacacc tgaagctcca
     2881 caagcagcta aagcagacaa aatacataaa aggactttca aaacaaaagg tgcttcaatc
     2941 cctaaagaat gtacagcagt caacgtcatc aaggaaatgg taatcgcggc accgcccata
     3001 ttgatcgtag cacctaacgg gatcgatatg gaataagact cttttgtcaa tttcaatctt
     3061 tcagccaaca tcatgtttat cgggatattt gcggcagaac ttcgggtaaa gaaagcggga
     3121 agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg gattttcttt aataaacaaa
     3181 aacgtgagga atgggtacac aactaatgcg acaaacagca tcgtaccgat caaaacaagt
     3241 aaaagctgca gatactctgc caatccagcg attccagtcg tagcaattga ttgataaacc
     3301 aatcctaaaa tccctatcgg agcaaatgca ataatcactt gtacgacttg tgacaacgct
     3361 atagatatct gatccaccaa ctcttttgtc gattctgaac gtgctcgaaa agctagtccc
     3421 aacaatacag cccagaataa gacgccaagg taattcgcat caataagaga ttgaacagga
     3481 ttttgtgtga cattcgtcaa catagtagat aaaacacttt ccaaactttg cggtgctttt
     3541 tcagatactg tttcttgtaa agtaatagga acagtaaaaa ttgaacttac aataactgct
     3601 acaagtgcag aaagcaatgt tcccactaaa tataagatca aaataggctt cacgaatgtc
     3661 ttattcccaa ttttatgctt ggcaattgac gccatcgtca agaaaaaaac cagaataggt
     3721 gcgattgcct tcaagcaact gacaaacaac gttcccaata ttgaaataaa tgtccatgtg
     3781 gggaaaaaga taccaaacaa tattccgact aaaataccaa caacaatacg ttgaactaac
     3841 gacatatcca aaaacttctc taacaaatga ccaccctttc acaattttaa caatcattgt
     3901 atcattttat gcaaagaaac taaaggtata attttctaaa aattctaaca atacaaacaa
     3961 ccatttcttg ctaattttgt actttttctg tctacacgtt gttttttgag atattctaag
     4021 ttaggatgtt attaatgggc aacttatcag gaaacgtttt cttatgtttt tgtccaattt
     4081 ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag agttatggct tcctgacaat
     4141 ctttgtcgct tgtataatga tggttttaca gcgtatattg atttcagcag tcactattcc
     4201 tacatatgca acaaaccctg gtcgattcta catctataca tttttacaaa cttccgttgt
     4261 tattggtgca aatttgatcc cactttacat gggttaccaa tatcaaaaaa tcgcccgttt
     4321 aaaagtttta cattatttaa gtcgttttgc ctttatattc ctaatagcca cactagtttg
     4381 caatatcttt ttttacgtac aagctggcag cttgaatatt cgtgattatt ggcttatcct
     4441 aacacctatc agtcagaatt actttactta tgctgtttct tgtactttgc ttttttgtag
     4501 tattccttat acagttggat ttttaaataa attatcgaaa acaacaatta aatatctact
     4561 attattttta acaattggat tagttggctg ctctactcta tttaataaag acccgtttgc
     4621 attacagaat ggacagagta ttttatggat tttttattta tttttaatgg gttatggttt
     4681 gaaagaatgg aactggaaag aaaaggtccg ctataagctt cctcaagtat ttatcagctt
     4741 gattgtttta ttcggtttga ttattttgat gacacaaatt tcactcatca tcagaggaaa
     4801 tacagataca gcgctgaggt tttctaaacc atattctctc ttcgcaatgt attatacttt
     4861 ctcttcgttt ttatttttag aaatgctatc tgataaatta ggtataaaag gaagaggaaa
     4921 ttttcttgct gtatttttga ttgtcactca agttatatgt aatttccctt tagtttctta
     4981 tcgcgtatct acatttatga aacgtgaatt tccaaactca gggaaggcat ggctgataaa
     5041 tatcgggcag ttttttattt gatatttatt agcagtgtcc ctcctagtta tctttttatt
     5101 ttatatacaa aaaatccgtt tcattcatcg tttcatcgaa tacttcgctt ttgattcgat
     5161 tgaacaatta actcagcgtt ggaaaaaaag aattcattgg ctttttgttc ataaaagtta
     5221 ttttttagtt gcagcatttt tttactgctt taccttcgtg cagattttcg ttttagaacc
     5281 aaaagaaggt tggaaagaaa ctatccaggt tgccttaaaa atatttactc agcgacaagc
     5341 acccatgatc ctgacaatca tcattattat gggatttttc ttcttactat tgttgatttc
     5401 caatcgtttt tggtacgcct tgactgctac cttgatcatt aacttactgc tcactatttc
     5461 gacagtaatc aaaatggaaa tgcgggaaga gcctgtcttt ccttcagatt taaaaatgct
     5521 gaccggattg tcagaactat tatcgatggt tagcccagtc ttattaatcg tgggcggatt
     5581 gatccttctc ctattattga ttactagtat tattgtacaa cgtagactac aaaaacagta
     5641 ttccttgaag attcattgga aacgtcgatt tatcagtatc gctgtattat taggatgttt
     5701 ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct ttcttgctgt ttaatttatt
     5761 taaagtaaac aagactttct ttaaccagca aggcgccgta aaagataacg gaccagtcat
     5821 tcaattccta aacaatatcg acattaaaat catggaagaa cctgctggct attcgaaaga
     5881 aaaaattaat ggaataatga aaaagtacaa cacaaaggca gatgaaatca accaaacaag
     5941 aagtgactgg ttaaacaatc agacgatcgt tctcaattta agtgaaagct tcagtgatcc
     6001 tgcacgtgtc cctaatctga cagtacctac agaccctatt ccttacatca aaagcattat
     6061 aacagaagga acaggaggat tgatgttatc cgtcggttat ggtggcggaa cagctaacat
     6121 ggaatgggaa ggactgacag gcttgagtat cagtaacctg tctgcttcgt tagttacacc
     6181 ttatacccag ctagtggagc gtcagaaaat aagtccaaat attaccgatc tattcgatga
     6241 aaaaatagcc atccatcctt ttacagctgc tctttatcgc agaaaacaag tgtttgataa
     6301 atttggtttt gacaggttct attatttaga cagtccggat aaattaactt atacggataa
     6361 aatagaacaa agcccacgca tatcggatga ttcagcatac aaagaaacct tgaaagcttt
     6421 acaaagcaat acggaaacgt cacaattcat ccaattatcc actatgcaaa accatatgcc
     6481 ttatgatgat tactatagtg aattgaacta cactgctgaa ggaactgctg tcattcctaa
     6541 tagaagaaaa gaattacaaa cttatatgca aggccttcac tataccgata cagcagtaaa
     6601 aaaattcatc caagaaatcg acaaaatcga taaaccgatt acctttgttt tttatggtga
     6661 ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa tatggattag aacagcacca
     6721 gacagattat ttcatttata acaacaagta tagtcgtgaa caagcagcta cgttaaataa
     6781 agaagttgtt gctcctaata actttggtgc tatggctctt gagcatgcga atatcaaagt
     6841 aactccttac tatgcgttgt tgacagaagt atctaatcat ctacctgcat taacaattga
     6901 tcctacagaa agcttgtcta atcgttttaa tggaaaacag gtctttgtta cagaggatag
     6961 tcaaattatc caagaaaaaa atttaactga gaaacaaaaa gaaattctag aagattatcg
     7021 tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa
     7081 aataaaagac taacaaataa actgagcaag gggttgtgat aaaagacatg tcacaacccc
     7141 ttgtttatct cattaatttg cttgtccata aacgatttga ttaaagccat ttttcaattc
     7201 attattcgct gtctcgttga agtaaagata attatcggga ttcccctgat tttgttgtgt
     7261 caccaatact tttggctgat tttgctgaaa cccgaataga tagacgtgct ttttcagata
     7321 tggttctgtc tctgcatcgg aatagacggc tgtgatttga tagtctgctt gacctgtccc
     7381 actttctgac cattctgcag aaacaggcac tccaccaata gccatcttcc actccccgtt
     7441 caaaatagct tgagggactt taagaccata taagtccaca ctcatttgat tcgtataact
     7501 tttgtattgt tgtcccattg tttggcccca tttggtcata aatgcctcta attgtgcgga
     7561 tttgtctgca ttccacagtg tttgttcaac ttgttcgctg ctttctgaag aggaagatga
     7621 agcctctaca gaagaacttg aagtagatgt ttgagttgtt tcagtcgttt tggaagactc
     7681 tgacgaagta gaggtatctg ctgtttctgt acttgctgca gttgacttgc tctctgtgct
     7741 agttgttgta gtagttgcaa ctgttgcttt cgctgtttct tttgttgatt cttttttcga
     7801 ttcattcccg ttggtacaac cagctaacaa aaaagctaga ccaattgtta ctaaaatcct
     7861 ttttttcaat taaaaaactc ctttcgatta ctcccacttc tttattctat caaataagca
     7921 atgtgatttt attgattttt cgtattttct catcaatctt tcaaatcttc taaaaactga
     7981 gaagcacccg ctgatgttgt ctgtaataca tcagcgggtg cttcttttat tttaatcttc
     8041 gtgattttcc aatttacctc acgctttata tttgtatttg aagaaacata gacttttagt
     8101 catcatcgtc ttcctcatcc aaatcttgtc ggacagtttt tccttggagt ctttgtactt
     8161 ggtagatgat ttctttattg aaacttaaaa gttcaacaag caattgttgc aagccatatt
     8221 tttcttcttt ttcagctaat ttgattgccc gtgtgataaa gacattttgg aaatcaaaat
     8281 cttttgcaaa gttttccaac attgttgagg ctggtaaata tttgttttga ccatgttcgt
     8341 aaatttttcc ataacgcatg acttcttcta aagtagacgg aacgatttca ttttcaatca
     8401 ctaactcatg agagatttta ttgaaccatc tcgcttcttg atcagcatat tctgaaaaaa
     8461 gggtacttaa ttcttgcgaa gatgtaccca aaacatacaa ttgtgcttgg atcaatttca
     8521 tttcttctaa tttaaaattt gctaatacat gccccaccat ggcgccagca gttggtttat
     8581 gatggtcgat atctgcttgt tttgtttctt gaacaaattt ttcttccact atattcattg
     8641 atttacgcct cttttacttt tgctgattgg acttcataac ctaatttttc cacaactgca
     8701 gctaattgtt cagggtggac ttcatctgga tcaaagtttg ctttgatttt gctcgcatta
     8761 aacaaaactt tcgtattgct taccccaggc tgttgagaaa ccgcatgttc gattttttgc
     8821 atacagcttg gacaagctag tgtatctaat tgcataataa tttttttcat cgtagttctt
     8881 ccttcctttt tcttgatagt cttattctag attacagctt ccataaaaaa attgattctt
     8941 atcaagtttt acgatttttc ttctttgctt tcgtgtccaa aatacaataa acgcatcgcg
     9001 ttaaagataa cgatcaagat actagcttcg tgaacgaaca tcccactagc catgtaaata
     9061 aacccggcaa ataagccgtt gagtaaaaat aagacgacac caatggcaat cactacattt
     9121 tcacgggtat ttaaaacagt tttcttagcc aagcggtagg catgaaccaa cgattcaaaa
     9181 ttagattgca tcaagacaac gtcactcgtt tcgatagcaa catccgttcc tgatcccata
     9241 gcaatcccga tatcagccgc agccaaagag ggagaatcat tgattccatc tccaataaaa
     9301 gcaacgcgca atccttgttc ttggtatttt ttcacaaatt gaaccttttc gtctggtaat
     9361 aattcagcat ggacttcatc aatgcctaac atatcagcta cataatcagc agtcatctga
     9421 ttatcacctg tcagcatcac taactgtttt gctcctgctt gtttcaactt agctaattgt
     9481 tcagcgactt ctggtcgaat cacatctgac acacctatga tttgagtgat ttttttgtcg
     9541 attgctacaa taatgaccga gctgcctgtt tgttgtaatt gatgtaaatc ttgtacttgc
     9601 tttggtgtga gagaaagttt gtgagcggca atcaatttgg tgttgcccgc acaaactgta
     9661 tgaccatcaa tctcagctgt gattccttgt cccttgattg tctgattatc tttgactgct
     9721 aattgttgaa aatcaatgtc tttctcctga gcataatgga tcacggcgcg ccccagagga
     9781 tgatcggata acgtttctaa tcgagccact aaagctaagc tgtccccttc atcctctgta
     9841 tagttcttga tagtggatac agcagtagtt cctttggtca atgtaccagt tttatcaaag
     9901 acaaaagtat cgattttgga aaaagtatcc attgcttcac cgcctttgac taagatgccc
     9961 cgtttcgctc cattaccgat tcctgctaca ttcgaaacag gcgcaccaat gactaaagcg
    10021 ccaggacaac caagaaccaa aacagtgatt gctaaacgaa aatcacgact aatcagacca
    10081 acaaccaacg caataagtaa aacggttgga gtataatact gagaaaatcg atcaatgaat
    10141 ttttccgcat gggatttggt atcttgtgct tcttctacta attcgataat tttggcaaag
    10201 gtcgtatcat ctccgacctt agttgcttca atcgtcacgt agccatctgc taaaatcgtt
    10261 ccagagaaga cttcatctcc actgattttg acgacttgtc gtgattcacc agtcactgcg
    10321 gcttcatccg catacccttt cccgtcaatg actcgtccgt ccacaggaat agctccacca
    10381 gtttttacta agacgatatc gccttcttcc acgtcatcga tttccacttc ttcagtcgtt
    10441 ccatcttctt ggacaacact agcagtagat ggggccatct cggtcaaagc cttgattgac
    10501 tcccgcgttt tggtcaatgt tttttgttcc agatagctac caaacaaaaa caagaatgta
    10561 acgatagctg actccttgta ttctccgatc ataaaggcac caagcacagc aatagtaacg
    10621 agtaaatcaa ttgagataac tttgactttc actgcctgat acgcatggat cataataggt
    10681 atgaccgcaa taatagaagc tatggctaga gcaatgttat agaacaagac aaaaccagta
    10741 aattttccga taaaaccaaa aataatcaac acagctgata aggcggtgat atgatttcga
    10801 tgctcttgga tccacttttg taatttcatc tttctccctc ttttcctttc attcttacga
    10861 gactaagtat agatacttat ccatcataaa aaattgacca ctatcaagtt tagtcaaaaa
    10921 ggcggttttc ttttggtaga atacactaca aaaaatagtt agcaagaaga ctcactaaaa
    10981 gtgatcttgc caactattgt cgtttctatc gaaattttca ttgaatgatt ttcctgtaca
    11041 gcatacatcg gagatctatt tctttatcta agatttttat cttgctaatt ttttccttta
    11101 gtctttcaaa tccacgtcgt tcatagaatt gatacgtaca gccactatct gtaaaaagga
    11161 atatttcttt tcctttctct cttttttcaa attctgttag caacttggta ccgatgcctt
    11221 ttgttttcaa atcaggattt gcagctaaaa aaatgatctc tccatctggc gaatgcttct
    11281 ttaaatatgc tgcaagcatt tgttcatttg ctgtctcgta agctccttta ctttctttag
    11341 caaatatatt ttgtaaaaaa tcgaatatct taacatatat ttgctgtcca aaagaacgat
    11401 gtttcttttt ttctcctttt atctccgcca gcaaaacacc tgccaactcg tcctctgcat
    11461 acaaagcaag aatttgagtt gctcgattca actccatata caaaaaatat cttccgtaaa
    11521 gattcagcag aagttcattt tccatatatc tctcaaagtt cattccttca acagcaaaat
    11581 agatagcttt tttgtaatct ttcttttcta agggtttaaa agttagtttc atcatttcgc
    11641 ctcctcaact tcaatgattg tgttatactt acattataat agacacagtg tctactatct
    11701 ataatcaatc atctataagt ttgtcgatta agcgacagct ttgctttgat atgtcggata
    11761 aggagatagg taaatgaaaa agacagatag acgcgtcaaa aaaacagaaa aagctttggc
    11821 tgaaacacta tccacattat tagtaaataa aaagattcaa gcaatcacta tacgcgaact
    11881 aacagaaacg gcagatgtac atcgttcgac tttttataca cattataagg atatctatga
    11941 tctatatgat caattagaaa gcaacttttt tcgggatcta aatgaaattc tctcttatga
    12001 tccagcccat agctatgagg aactatacac tcgcttgatt gactatgttt acgcaaatac
    12061 ttttttatat cgcttattta tcctcaacca tggtgatatt tcgtttcaga agaaagttaa
    12121 acaagttatt gaagaaaact atctgaacat atggctatat gaagacaaga aaacgattat
    12181 cacagaagaa atgagattct ttactacgta ccacgtccaa ggttgtctat ccgttctcaa
    12241 tcggtgggta gaaagcaatt acaagtactc caaaaaagaa ttattagaac tcttacagaa
    12301 actgaatgat cacatagaga aaatcatgcc ttgaataaaa acatactaag cttagttaag
    12361 aagaaccgta accttctcgt ataacaactg aatcttattt agccaaattg tttactgaag
    12421 attttttgag taaaatttct ataattccta cttattgaaa agtagtttgt agtgctgctt
    12481 ctccctctct aagtgatata tcttgttgta ccaatcaaat tcacctcatc attaataaat
    12541 tacttttagg ctaatttatt acaacttata ggttgaagct gtaaccaatt tatagtcata
    12601 ttatattttt atcatccagt ttattgaatt cttctcgttt acctcaattc attttaaagg
    12661 ggaatcagct catcatctaa cattttacgc catgctcgct tcatttcttg tgggtattta
    12721 aatggtttcg cttctggatg tttggcttta tatccttcgt aatccttact gttgataatt
    12781 gctttcatat taggaattgg atcttcacct ggcatatatt gcagattaga agcatgtaat
    12841 tttttctcag aaacaaacca ctcattggaa aagacatgaa tcaaatcatc acttacttgt
    12901 gtaaaatagc gacgtttaat gacaaagata tcttcattgc tatctagtgt accttcattt
    12961 ctttgtgctt aaattcaaag ataattttct caataaagat ttttgattcc agtaagattc
    13021 ctttactcct tttacaacat agcgttgtcc tttgttttga aacatcttct tcccctcttt
    13081 gccattaaaa atagcccaat tctctataaa gaagattaag cttcattatt ataatataaa
    13141 gtttaaattt ggtttattac ttactttttc ttattctcta gtaagctaat attgaaaaaa
    13201 atgtaatcag atcattattt ttatatgttg taacttcctg ataagaaaac taattaaaag
    13261 aaagaggtaa ctacataaac taattaaaaa agctaacttc atagttggag gataaatgga
    13321 agaaacttca atgattgttt ctccttgtgt tccttcaatc gacactagtc ctattatcaa
    13381 tctctctttt ttgtccattt acttttgcga ctgttcccca ataatcaaat acaggaagcg
    13441 ttattttttg tcctttagat tcatgtactg tatagactat tttcgaatcc atcacttgat
    13501 aaggcatagt atgccattcc ttgccaattc ttacttcatg attctgcaaa tgttctttgt
    13561 attgtaatgc ttccgtgggt gcatagtcat aaagtcctcc gggaaggagt gtcgtgtaac
    13621 gaataagatt ttcttgattc attaccaagt gatctctctg agtgacaact ttatgttcat
    13681 agtttgcttt tgttgcagca gatacagcca acagtaataa aacaattgcc gttatattaa
    13741 ctacttttct ttgcttagct gatgtgctct tcatgaaaac gaacgataaa atcaacgagc
    13801 caaacagtac ttgaaagcct aagattcgcc aaggaaattg aatcatttga atgggtgtat
    13861 tttgtaataa agaccatgga aaaaatttgg tggataagag ccaagagact accgcactaa
    13921 ggtaaatcac tttttctttg cgattaaatc ctttaaaaaa gaagagtgga cagatcaaag
    13981 aaataatacc aatgattcct accgtaaagg ttgtcaggtc attattcatc gcaccaatca
    14041 aactatccga gacatttaat gcttggtgtt gtaaggtcgt tttatctggc tcattaatcg
    14101 attgaaccat gatttgtcgg aacatcggat accaaaagta tgctgttaaa agaatcgtca
    14161 ttcctactgc ctttgacaac tccacaatca accggatgct ttcttttttt cttgtttcac
    14221 ttactacaaa gccgcctaag actagtaagc caacggctat tacactcata aaagtggaaa
    14281 gaacatgtgt ataaatgagt aagctcatac tgactgctaa tggaattgca catggctttt
    14341 tctcataaat cagtgcgtaa gcataataca taactatcgg tataaccgta agtgcaatcg
    14401 cttcagcaat agcagaacga tgataaatat caatcgtacg gtacccataa aatgtatata
    14461 aacagctaaa tataaaagcc gcatcgattc tttttaaaaa ttttagtcca taatgaaaca
    14521 tcagcatgat agtacaaaca ttcaatagcc aaacatataa gatataacta acgattaaat
    14581 tattacttat ccaataaaaa atgactgccg gaaaaaaggt caagtaggga taaaaataat
    14641 tcactccgct cccataatga ttaaacgtcg taaaattgat tggaccagaa aaaattgaag
    14701 ataacccttt gactctagca aaatggaagg caatatcccc cgatgtagaa atagtatgaa
    14761 catttctaaa ataattgatt gaataaagtc cagcaataat tgctaggaga atggacaata
    14821 ttattttttt ctttacttgc attacttaat tatatactcc tttcatggat tgctttatct
    14881 agtataactt aaattttaaa taaatcgtta tttattttta ctctcactta aaaaagcact
    14941 aaaattataa gagagggagg taatggtctg aacaccaaga attgttccaa catcgaattg
    15001 cttaactaat gagttcaaag cttaattatg tggctcttat atttaccgag cgtggattgc
    15061 gatctcttta gcgaaacaaa catctttcat aaaaaaaaca gccatatccg ctttcttgga
    15121 tatgactatt tactactact ttactatctt tcattcttct tgtctcactt atgataaggc
    15181 gctgcgtatc atgagtgaat gatattagga aaacttcata aaataaatct tttatacatt
    15241 tccacttcta gatggtcatt catgttagaa gaatcaaact atttttttac tgtcgtaata
    15301 atattaaaac actagaacaa atcaattaaa ttccataaaa tataaataat agacctagca
    15361 tcatctggta taaaatggat gctaggtccg ttatatttac tcaaaattga ttctacgaat
    15421 agatcttctt atcatttcta ttttagtagt catgcgttgt tgttcttcta aaggtggtaa
    15481 tggtataagt agtttagata aatttgtacc atttacattt ggttgcccag ttccgtaaga
    15541 aattttttct aataacttcc aatataatgg cgagtctaag aagcagtcta cgtattctga
    15601 taataatttc tcaactaacc taattcgaat caagtaagaa gcaaaaacag attcctcact
    15661 tatttcctta accaagaaac tttttcccat agttcctcct gtccttgcta tcaaaatgtc
    15721 attttcttcc aatcttaaat caactagttt agaattacta atatcacagt aaggcacact
    15781 ggaccaattt acacgtccct cttgtatatc tgtgattctc aaaaacttaa cattgccttg
    15841 ttttttcgcc gaatctgtat agccatattg aatcttagta gaaatacttc ctaaataaac
    15901 ccatagtcga acatcgctag atgatttttc ttgtcgttct ttatgaacaa cagtgatcat
    15961 cttgagttgt ccgactattt tttagttgca gcaatctttt tgggacgctt atttgcatga
    16021 agcaacgttg ctaagtctag taataaaaca ttggctaaca caggattggc tttctcgcga
    16081 tacgctgcgg cgccactttc aaacaatact gctagacgat cttctacaac aacgatttgt
    16141 tccaaatacg gtggaaattt gattcgtttt aggatccggc taggagagtc ccgctcggac
    16201 actaagagtt ccgaggtata aggaccataa gaacgactgg ctaagagata atggccataa
    16261 aaagccacac cttgaatctt atcagatgtt tttctgctca ttgtcacggt tggttggatt
    16321 ttgtctttgc ggcgaattgc tgttttcatt tcagctatcg tcggcaatag atagaaagtc
    16381 aactgtcctt caccttttag tgtgaagttg ccagatacca gttgatcatt gttttgagtc
    16441 aaatacgatg cttgtggaat ctctgagaga tcagttgtca gccaataagc gattggctgt
    16501 cccgtctctt cagatgtatc tgctaagata tcgatcaaac gtaaagcggc tactcgtcca
    16561 gtagcagatc ctgtgattgt caaccataaa agttcccgtt ttgtatcata tactaagccc
    16621 cctacatgtg gccgtttagg taaaatcagc agtttgatcg gctgtcctgt ttctctatcc
    16681 agtacatgaa taatagaatg atgctgatgg tcatagcagt aggcagaaat aaacacaaaa
    16741 tcttgagaaa aagccaaccc ttgcggcgtc atccattgac aaacctcatt ttcgccggtt
    16801 tccggcacca ccattcttgt ttctgttaat ccaggaatgt tatattgttt tgcttcagcc
    16861 ttttcccaag ctgcttgaac aataggatac cgttttaact ttttttcaat atcctgatgt
    16921 atccgttgct gcttccaatg cttaaacttt aggacatagt aaacaagaag gccaatgacc
    16981 agcaacacta aaatcattat tgctacccgc tgtgtcattt ttttgcctcc ttgttctatt
    17041 cgatcaatcg tccatatacc gctattaatt accgtaacta catgtttctt ttactagcgt
    17101 ttctttatag gatctttcat aatctcattt atagtaacat taagaatccc ttattctact
    17161 atagtcactt ccacatcaat attgcctctt gttgctttag agtatgggca aaatgcgtgg
    17221 gcttcttctg cgattttctc tgcttggtcc ttctcaagtc ctggcaaaga aacttctaaa
    17281 acgaccccga tttggtagcc cccatcttct aattgaaata aagatacccg tgctttgact
    17341 tcagaatcag actcaagatt attttcttcc agcatgtgtt gaaccgcgcc gttgaagcaa
    17401 gatgcgtatc cagccgcaaa aagttgttcc ggatttgtcc cttcactatg gattcctggg
    17461 cggtcgatct tcatgtgcat acttccgttt ggtgcctcaa cttcgccatc acggcctcca
    17521 cgattgatca ttgctgtttc gtacattttt ttcattgaga tcatcctttc tttcgtcgtt
    17581 ctatattaag tgtaacgagt attttttaat ctgacaaagg taatgcttgc attcggcgac
    17641 atcagttaga caacatcgat gatcagtgat agaagcagtt tatctataca aaaaaagaag
    17701 ggttctatga tatttggtgt ccttactcgc cttgagtaac aacaccaaat atcatagaac
    17761 cacttaatca gcttcacact gctttttcta ctttcgtagt tgaaaaaaac ttaaacaaat
    17821 catcggcaaa tagttaaaga aaaaacttaa tcgttgaaag aagcccaatt gcttaaagaa
    17881 ggcaaactga ctaatcgacg gaatccgtgc taatccgtaa agacctgcaa ttagtaaact
    17941 aataactgtt aaaacaagat aaaaatggct aagcgtacct tgaccacttt gacgataaag
    18001 cagaacaagt aacaatggga aaagtagaaa gcctgcatag cctagcccag aacctatatt
    18061 atggatccaa gtcgatagat tacaagagct ttcattcgta ttgatactga ataagccagt
    18121 aaaaatacaa tcacctatcc catataaacc aactgttgca acaacaagta ttgcaagtgt
    18181 ttttgatgtt ccaacaaata ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc
    18241 agatacaact gaccagacca agaaagcacg tctgactgga ctatctacgt caccaaacac
    18301 actaattacg gtagtcattt gattcagttt gggataaaaa aggccaagga tatacggtgt
    18361 cgataaatca ctcatcacac ctaataataa aaaataaaaa ccatattttt gcaaaaaaat
    18421 cattttcttc ccctactttt tagtgattat tcgatacatc caacaatcaa attttttctc
    18481 attttaacac atatttttat tctagttgtc ttttgaacaa tcaataacat aaaggtgagt
    18541 ttcttctaca ttttaaaaaa tcgaatggca ctgaagagtt cagttgtcag catcaatctc
    18601 ctactcaaaa aggcaaaaaa acgtaagtca gatgttgttg acatctcact tacgcatcca
    18661 ttgattgaaa atttcttagt aaaatctact agcctaaaaa tttgaagact tcttctcgat
    18721 catacagttt aaatactttc tcgcataaaa attgagtaat tagtgccgca aaaacaggga
    18781 tgactaacca acaaagaaca acaagaaagc catttagccc tgcatctaaa gaagccaacg
    18841 gaccaacaag tccgaccacg ccgaaaccag cagaagcagg cgtacctgaa atagaaaata
    18901 gagcgacagg aatagctgta atcactgcac taactaaaca aggaataaga atcacaggtt
    18961 ttctaaatag attaggcatc atcatcttca tagcgcctaa agcaatcgca attgtcacac
    19021 ctgacttatt aaccttccaa gagttcacta ctaaaacaaa agtggttgca gctacgccca
    19081 tcgcagccgc accagcagaa acaccatcta gttggattgc cattccgatt gctactgttg
    19141 aaataggaga gataatcaaa aaggcaaagg aacaaccaat caagatagac attaacagcg
    19201 gttgtaaggt agtgaagtta ttaatcaatg atccaattgc agaagtaatt gctgttacaa
    19261 aagggaataa ataaaaacca agtaaacctg cacctacacc tacaacaatc ggtgttaaca
    19321 caatcgacac tgacccaaaa ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg
    19381 ctgtcagcat cgtattaata atatcgcctg taccagcacc aacgtaaaca cccgtttcac
    19441 ccaaatcagg tgtgaatttg actacaccag aaccaacaaa agaagctccg gctacaacca
    19501 tcattctcat cggatcaaat ccaaattgta aggcaatcaa accaccaatg atcaaaggtg
    19561 ttgctagttg aaaaataact gctaactgtg cgatcgttac cgcaaaggga tacgcactaa
    19621 aatattttaa aatggcacct aatactgcat taggaatcaa tccaataatg attcctgttg
    19681 ctgtaccagc gaggacttta ttcagaaaaa ttttcggtga tattttttgt gaagtttcca
    19741 ttcaatctct cctttatttt acgttcaaac tatcttcttt tgcatggaca agctcagtca
    19801 agaaatcaca ataaggcgtc gcaacaccat atttcttccc ttttctagaa attgcgccat
    19861 tgatataatc gatttctgtt aatcgatggt tcttaatcaa gtcttgatac attgaaggat
    19921 aatgtaaccc aattgtttcc gggtcaaaac aagattcaca atgtgcaatg acttctggga
    19981 catctagttc aatcttctct actgctgcta ctttggcaaa ctcattgaca atcgtcgcca
    20041 ccattttatg agcagtcgat gtttttccta gctcagccat attaacgtct aagattgtgc
    20101 atagtccgtt cattgttccg ttcacacatg ctttgcgata aatagaatag tgaatattgt
    20161 cagaaaaatg agcatttaat cctgattcag acaatttgtc tgctaatttt tttgcagctg
    20221 cttcttttcc atcacctaaa ttttgtaatt ctactgaacc acttccaaat aatttgacct
    20281 gaccaggacc ttcaagacct gccgtccaca tggtatttcc aatatagata ttttccattg
    20341 gtacaaattt ctcaataata tcttcatgcc cgataccatt taatagacat aagacctctg
    20401 tatctttttt gattaatgat tggatatcct gcagcatttt ttccagctgc atcgctttgg
    20461 taaatagaat aatcagatca acttgatctt ctttttctac ttcggattga agaacgattg
    20521 gtagttttgc ttctacctct ttaccattaa aatttgcttg taatccatgt tctttgattt
    20581 gttggacatg ttctgcccag ccatcgatta ataatacttc gttcccactt tggtgaagca
    20641 tcaacccaaa gcgacttccc attgcgccag cgcctgcaat tgctattttc attgattctt
    20701 cctccttttt ctttctatta tactcatttg agaatctttt cacaacctcg ttttgttatt
    20761 cttctcttat ttatcatgtt tatatccacc attcaagtta ttttatcagc aaatcaataa
    20821 aaactggaat agaatcccaa tttcatcgta agagaaacta gactagcaat aagaaaatag
    20881 aaacattcat agaaaaaatc tcactcctct gattataaaa gagggggcat taacctcgtg
    20941 tatactaaaa ccttccattc attcgatgga tgattttctt tttgtgaaaa aatattcatt
    21001 gtagtacgat gttgcgcaac acagacggat ggtattgctt attatttatt aaaatcgaac
    21061 aatacactta tttaaaaact tctttcccag attttcttc
//
LOCUS       DAEGIQ010000044        20962 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000044 DAEGIQ010000000
VERSION     DAEGIQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.044"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            598..1287
                     /locus_tag="KMT45_001450"
     CDS             598..1287
                     /locus_tag="KMT45_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7004918.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="KMT45_001451"
     CDS             1274..2482
                     /locus_tag="KMT45_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBE7004919.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="KMT45_001452"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="KMT45_001452"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBE7004920.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="KMT45_001453"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="KMT45_001453"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBE7004921.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="KMT45_001454"
     CDS             complement(6585..7280)
                     /locus_tag="KMT45_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBE7004922.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="KMT45_001455"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="KMT45_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBE7004923.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="KMT45_001456"
     CDS             complement(8479..8670)
                     /locus_tag="KMT45_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBE7004924.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="KMT45_001457"
     CDS             complement(8691..9581)
                     /locus_tag="KMT45_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBE7004925.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="KMT45_001458"
     CDS             complement(9568..10335)
                     /locus_tag="KMT45_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBE7004926.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="KMT45_001459"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="KMT45_001459"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBE7004927.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="KMT45_001460"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="KMT45_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBE7004928.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="KMT45_001461"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="KMT45_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBE7004929.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="KMT45_001462"
     CDS             14906..15289
                     /locus_tag="KMT45_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004930.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="KMT45_001463"
     CDS             complement(15457..16215)
                     /locus_tag="KMT45_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBE7004931.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="KMT45_001464"
     CDS             complement(16233..17051)
                     /locus_tag="KMT45_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBE7004932.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="KMT45_001465"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="KMT45_001465"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBE7004933.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="KMT45_001466"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="KMT45_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBE7004934.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="KMT45_001467"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="KMT45_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBE7004935.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="KMT45_001468"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="KMT45_001468"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBE7004936.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5992 a         4266 c         3838 g         6866 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttgttt aaatcacctt tt
//
LOCUS       DAEGIQ010000045        20621 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000045 DAEGIQ010000000
VERSION     DAEGIQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.045"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            221..427
                     /locus_tag="KMT45_001469"
     CDS             221..427
                     /locus_tag="KMT45_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004937.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            623..790
                     /locus_tag="KMT45_001470"
     CDS             623..790
                     /locus_tag="KMT45_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBE7004938.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            816..1160
                     /locus_tag="KMT45_001471"
     CDS             816..1160
                     /locus_tag="KMT45_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE7004939.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1165..1446
                     /locus_tag="KMT45_001472"
     CDS             1165..1446
                     /locus_tag="KMT45_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004940.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1549..1863
                     /locus_tag="KMT45_001473"
     CDS             1549..1863
                     /locus_tag="KMT45_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBE7004941.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1841..3535
                     /locus_tag="KMT45_001474"
     CDS             1841..3535
                     /locus_tag="KMT45_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBE7004942.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3555..4733
                     /locus_tag="KMT45_001475"
     CDS             3555..4733
                     /locus_tag="KMT45_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBE7004943.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4696..5382
                     /locus_tag="KMT45_001476"
     CDS             4696..5382
                     /locus_tag="KMT45_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBE7004944.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            5382..6542
                     /locus_tag="KMT45_001477"
     CDS             5382..6542
                     /locus_tag="KMT45_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBE7004945.1"
                     /translation="MTIKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6552..7427
                     /locus_tag="KMT45_001478"
     CDS             6552..7427
                     /locus_tag="KMT45_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004946.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7424..7735
                     /locus_tag="KMT45_001479"
     CDS             7424..7735
                     /locus_tag="KMT45_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004947.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7725..8078
                     /locus_tag="KMT45_001480"
     CDS             7725..8078
                     /locus_tag="KMT45_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBE7004948.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8068..8469
                     /locus_tag="KMT45_001481"
     CDS             8068..8469
                     /locus_tag="KMT45_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004949.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8462..8866
                     /locus_tag="KMT45_001482"
     CDS             8462..8866
                     /locus_tag="KMT45_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004950.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8878..9486
                     /locus_tag="KMT45_001483"
     CDS             8878..9486
                     /locus_tag="KMT45_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE7004951.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGAAEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     SEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELEAKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVATTATQSQ"
     gene            9506..9868
                     /locus_tag="KMT45_001484"
     CDS             9506..9868
                     /locus_tag="KMT45_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004952.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9871..10053
                     /locus_tag="KMT45_001485"
     CDS             9871..10053
                     /locus_tag="KMT45_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004953.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10071..13391
                     /locus_tag="KMT45_001486"
     CDS             10071..13391
                     /locus_tag="KMT45_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE7004954.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            13442..14179
                     /locus_tag="KMT45_001487"
     CDS             13442..14179
                     /locus_tag="KMT45_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE7004955.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFTLNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14189..16480
                     /locus_tag="KMT45_001488"
     CDS             14189..16480
                     /locus_tag="KMT45_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE7004956.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDSIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16504..18606
                     /locus_tag="KMT45_001489"
     CDS             16504..18606
                     /locus_tag="KMT45_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004957.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSINFGDYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGA
                     DYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTTPTFSPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGSTATPFQPNLLAEPYNMCREYPNENIADHTVKF
                     PIESGDHQIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLT
                     DVWSLTFTPTNVVASSPKLFRVYQYPQSTVGACRIDWLKIEKGDTRTPNISEYKYRGT
                     GMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINE
                     VDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVS
                     IYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKW
                     RRNSSYGSDYTFAITYTI"
     gene            18623..18772
                     /locus_tag="KMT45_001490"
     CDS             18623..18772
                     /locus_tag="KMT45_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004958.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            18769..19215
                     /locus_tag="KMT45_001491"
     CDS             18769..19215
                     /locus_tag="KMT45_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004959.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19217..19354
                     /locus_tag="KMT45_001492"
     CDS             19217..19354
                     /locus_tag="KMT45_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBE7004960.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            19392..19685
                     /locus_tag="KMT45_001493"
     CDS             19392..19685
                     /locus_tag="KMT45_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004961.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            19682..19906
                     /locus_tag="KMT45_001494"
     CDS             19682..19906
                     /locus_tag="KMT45_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBE7004962.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            19903..>20621
                     /locus_tag="KMT45_001495"
     CDS             19903..>20621
                     /locus_tag="KMT45_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBE7004963.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIE"
BASE COUNT         7284 a         3117 c         4366 g         5854 t
ORIGIN      
        1 atcaaagcaa tgttattttg ttgctgtcta ttgtttttta attattcaca cgataactaa
       61 aggtgggtga agagaaatga ttccattaat aatttcaatt tttgcgctct gtcttaatgt
      121 ctatatgatt ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa gaataatttt
      181 atagtgtcac tgtggcggaa agggtagacg ctaagcatgt gtgctaggtc aatgcttcgg
      241 caaccatgca aggttcgatt ccttgccagc gacattaaat gcctatgacg gttacgacta
      301 ccgaaaaaag atcgttaaga agctatacgg tgctacgtac ggcaatgtag taagtgtgct
      361 atctgtacac caccaagctt cggtcactgt ggcggaagta gaagacgcag cggtaaatgg
      421 cgagtagcct cgtgagagcc tggtaagttc tcgtgagtgg tgcaatccca ctccagcgac
      481 tttaagcaac cgaggcatcg gcggtttaaa aatatagggg tgcgcaattt cgtacgcgtt
      541 ttgtgcatcg tgcaagttac tattacatat tagatcactc tttgagtggt ctttttattt
      601 ttgcacaaag gaggaaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
      661 aatcactgtt cataaaggta atggcgaaaa agtatatgag tatagaagac caataaagag
      721 cgatacatat aaacgaaagg aaaacaatga agttattcca ttgtatggca aaagaatagc
      781 taagcattaa ataagattgc gaaaggagac ggaacatgac cgaggaattc tatagatggc
      841 tattacagtt gacaagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
      901 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
      961 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     1021 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     1081 gcagatatct tacagcatta gataaacaag agaagaagat agaaagcttt gctaacttcg
     1141 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     1201 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     1261 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     1321 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     1381 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     1441 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     1501 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     1561 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     1621 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     1681 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     1741 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     1801 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     1861 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     1921 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     1981 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     2041 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     2101 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     2161 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     2221 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     2281 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     2341 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     2401 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     2461 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     2521 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     2581 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     2641 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     2701 aactattaat tacaatgcag atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     2761 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     2821 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     2881 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     2941 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     3001 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     3061 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     3121 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     3181 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     3241 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     3301 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     3361 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     3421 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     3481 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     3541 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     3601 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     3661 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     3721 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     3781 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     3841 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     3901 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     3961 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     4021 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     4081 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     4141 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     4201 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     4261 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     4321 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     4381 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     4441 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     4501 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     4561 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     4621 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     4681 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     4741 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     4801 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     4861 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     4921 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     4981 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     5041 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     5101 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     5161 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
     5221 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
     5281 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
     5341 gcagcattaa ataaaacatt tggacaagga gatgcaaaat aatgacaatt aaaaatttaa
     5401 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
     5461 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
     5521 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
     5581 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
     5641 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
     5701 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
     5761 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
     5821 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
     5881 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
     5941 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
     6001 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
     6061 ctattacagc tttagatgct gtaactatgg gcggtttgat ggcgcgacta tcaaaattca
     6121 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
     6181 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
     6241 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
     6301 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
     6361 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
     6421 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
     6481 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
     6541 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
     6601 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
     6661 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
     6721 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
     6781 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
     6841 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
     6901 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
     6961 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
     7021 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
     7081 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
     7141 tacaaatctg gacagccgag atgtgggcac agctttggaa catgatgtat ttcaatattg
     7201 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
     7261 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
     7321 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
     7381 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
     7441 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
     7501 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
     7561 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
     7621 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
     7681 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
     7741 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
     7801 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
     7861 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
     7921 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
     7981 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
     8041 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
     8101 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
     8161 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
     8221 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
     8281 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
     8341 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
     8401 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
     8461 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
     8521 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
     8581 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
     8641 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
     8701 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
     8761 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
     8821 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
     8881 gctaaaacaa aaactgtagt aacaacgttc gataacataa gtatcaaacg aattgctttt
     8941 aattttaaga acgcaggaaa tgcaatcgca acgaattgta acggacaatt agatggcgaa
     9001 acagaaatgc aaacggtggt taaaaaatgt ggagcggcag aagtaaaatc aaaatctaaa
     9061 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
     9121 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
     9181 gtaagcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
     9241 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
     9301 gcggatgaag tagctgcgtt agaactagaa gcaaaagtta tgcaagatga atttggtaaa
     9361 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
     9421 aatctatctg ctgatgtgat taaaaaaact tcagttgtgg caactacggc cactcaatca
     9481 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
     9541 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
     9601 agattttaac acagacgagt ttgcaaaaat gtcgatggga aaaggctcta tggatattac
     9661 agttattcaa ggtgcaaaag ccgtatatgt agcttatcgt caagcgaaca tggtcgatta
     9721 catttcattc gatgaattta tagataaatg ggattttgat atggaggttg cagtagctgt
     9781 atacagtact atgatgttca aacaagcacg tgatgcctat caaaaagaat tcgaaaaagc
     9841 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
     9901 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
     9961 atctgtttag atgacatttt gcaaatggct tacaacaaga gcgcttatga aggatggaag
    10021 aactatgcaa taaatcaatc ccagaaaaac taaaggaaag gaggtaaaaa atggctaaaa
    10081 agagaacgga agcagaagta actttcatag ctaacgatga cggattgaaa tctacgttaa
    10141 aagaaatcag tgctgaatta actaaaaata gagcagaatt aaaactagaa caagctcaat
    10201 tacaacagac tggttctgaa tcagacaagt taggaagtaa attatcttct ttagaaaagc
    10261 agtatgaatt acaaagtcaa aaagttgaag taactagtca acgtttagcc aatgccaaaa
    10321 aatattatgg agaaaattcc accgaagttc agaaacttga aagagaactg attaatcaac
    10381 aaacagctca gcaaaggtta tctaatgata tagataaaac gagtaaggca ttggctcaat
    10441 ctaaaggtga atttaaaacg tatgagtcaa ctatgaaaga cttagataat gagcaacaac
    10501 atctaaagtc aagcgcatca ttagtagaat ccgaatataa aaaatggcaa gcaactgctg
    10561 gccagtctgc tacagaatct gaaaaattag caaaagcaca agaatatgtc ggcaaacaga
    10621 gtgatattgc tgaacaaaaa atcgaagtgc taaaaaaaca attagatgcc acacaaaaag
    10681 aatttggaga aacatctacc gaagctttag aaatgaaaac taagcttaat gatgcggaaa
    10741 gagaattcga agaattaagt aatgctgcta aaaacgttga tacctccaca gtagatgata
    10801 tcggcaaaaa acttgatatg ggtaatttaa tggaagctac tgatcacttg tcagtgatcg
    10861 gagataagct tattgatgta ggtagtaagt ctattgaagc agctggaaaa gcacaagcta
    10921 tgcaggccca atttaaacaa gtctttggct ctttagaagg ggaagcacag gacgccgttg
    10981 agggaatggc tgaagaattt ggaatgttac caaatacgat caagcctgtt tttacacaat
    11041 atacgtcaat gtttaaagga cttggatatg ataccaaaga agctatggag ttagctggtg
    11101 atagtactca gttagcagca gatgcagcag ctttttacga taagtctatg gatgatgcta
    11161 gtgaatctct taattcattt ataaaaggga actacgaagg tggggagcaa ataggtttat
    11221 ttgctaatga tactcaaatg gcagcttatg ctgttaagca taatttgata ccagcgactg
    11281 aaggagcaaa aaaagccagt gaagagtcat tgttagctgt tgaaaaagca caatctaagt
    11341 atgctgatgc tattaagaaa catggtgaag gatctttaga agcaagagag gctgctttaa
    11401 aacttaaaga tgcgcaagat aaaataaatg aagaattagg cccacaaacg caaaaatggt
    11461 ctgatttaga tgaggctacc aagcaagcag ttcgagttca atatgctgaa gatatgcaaa
    11521 aattagccgg tgctacaggt caggctagta gggagtctga tggtttagaa aaccaaatga
    11581 caagagcaaa gcaagcgtta gaagattttt atgcctcatt gggtgaagat atactgcctg
    11641 tgtttatcaa agggttgcaa gcaggagcga aagctttgca aggattagcc gaatggtgga
    11701 gtaaacttga tgggccaatg aaaaatttca ttttagctct tggaggaatt ctagcattat
    11761 taagcacatt agctcctgtt ataaccgcag ttgttacgat agttggcaca tttggttcta
    11821 cagttttgct gccaataata ggaattatag cgggcgttgc agctgtaata gcgattgtaa
    11881 ttacagcgtt tcaaaactgg ggcgcaatca ctgactggtt tagtgattta tggaaaaaat
    11941 ttaccgattg gttaggtgat acctgggaaa gtataaaaga aagtgcctca tcagtttggg
    12001 atggagtcaa agaaacctgg tctggatttg tagattgggt tcaagaaatt tggcaaggag
    12061 tttctgattg gtttggagag ttatggagcg gattagttga aggagcttcc aacatctggc
    12121 aaggagtcca agagacttgg caagcattcg ttgattgggt ttcaaatatt tggaacggag
    12181 tcaaagaagt atggtcgatt atttgggcag acattgtagg aattgttcaa ataccatgga
    12241 cattaataac gtcattgatt caagctggta ttaatattat cgtgggtatt tttgatgtag
    12301 ctggacagtt attaggcgca gcttggcaag ctgtttggac acctatttct gatttcctta
    12361 aaaatacttg ggatactatg acacaatgga taagcatcgc ttggaatgga attgtaacta
    12421 cattccatac tatatttgat ccagtagtgg catggtggaa tggtatatgg acatctatta
    12481 gtactacggc ttcaaatatt tggaactcaa ttagtgcaac agcttctagt atttggaata
    12541 gtatcaagaa tacaatcact agcttggtac aagcagctgc tacagtaatt caaaatattt
    12601 ggtcaactgt atctagttgg ttaggtggaa tttggaattc aatcagctct acagcatcaa
    12661 atatctggag tagcgtgact agcagtataa gcaatgctat aaacgcagct aaaagtgcca
    12721 ttcaaagtgt ttggaatagt atatcttcgt ggatcagcgg aatttggaac ggtatcaaaa
    12781 acactgcttt gaatctttgg aatggaatta caagcactat tagctctaaa gtaaacgatg
    12841 gaaaaaatgc aatttcaagc ggttggtcca atctaacagg tattgtttcc gacatattca
    12901 ataatgttaa aagcacgata gctaatatat gggaaggtat caaaaagact gttagcgctc
    12961 cgattgattg gattagagat aaaatcagta gtgtctttga taatttgaat atttcgatac
    13021 cacatattcc gttaccacat tttaaattga gcggagaatt caatccattg aaggggaaaa
    13081 tcccaacatt gggtgttgat tggtatgcga aaggtagtgt gtttaattct ccaaatatta
    13141 tcggtgtcgg tgaagcagga cctgaagcag ttttaccttt gaaaagatct gtgctgcaag
    13201 aaattgggga tcgtatcttg agtagcacat cagtttcatc tagggcacaa acgattcaac
    13261 ctgtgaataa ctacgaattc aatttcacaa ttgatggtaa cgcagatgag gttactatga
    13321 agcaaacaac tcaacaaatc attgatagca ttacaaaagt tcaaaatgat aatgcttcgg
    13381 catggcgtta aacaggagag tatttctcct gtttttttag ttttaaaaag gatgtgaaaa
    13441 aatgactgat tgtatacatt ctataatcga tggatttcct gattatttgc ataaattggc
    13501 tttagcggaa agaccaacca taccttctcc aaaaagacag agagttgaaa cttctgtttt
    13561 aggacggcta ggtggcttag tacaagatta ctcgtttgaa gacatgtcgt ttacattgca
    13621 ctataactat ttagaagatg tggaagacca tcaagcattc aagcaatcgt tttatatcat
    13681 gcgtcattgg ttaaactatg caaagaaatt agaattctct gatgatccca acgtctatta
    13741 tgttatccag actatcgata ttggggatgc agaaaacgat attgttgaat ggggagagtt
    13801 cgatgtaaat atcactgcga aaccattcgc aagagttcaa gaagatgtac ctataaccgt
    13861 agataaacca cagtcattta acttgctgaa taatagttta gaagaaagtt ttccaaagat
    13921 tatcatcact ccttcagcta cttcatgcca attcacctta aatgattatg tgtttagttt
    13981 tgaaggctta gtagtgggaa ctgacatagt cattgatagt gatttgatgc tttgctacga
    14041 agagcaatcg gacggagata ttttagatcg gtccaacaaa atgaagacca tgcaatatcc
    14101 aacgttgcaa gtggatatta atcattttaa ttgtactggt ttgagcaaaa tacaaattta
    14161 tcgtaatggg ttaaggtagg tgaaatagat gatcgataat ttactaacta tttacgataa
    14221 aaatgacgcg aataatttag ctgaacattt atatgatacg caaggtttag gcgctttgtc
    14281 agactggtta acagctactg ttagcaataa actaaacgga gccgagatat ttcagggtac
    14341 ttattcaata aacggaacta atgcagattt gattgtagaa ggacgtatta ttcagtgtta
    14401 tgtagatgaa aatcgagcaa aacagcgtct acggatttat tatgcaaaaa cttctgtaat
    14461 aggcaatacg atagaagtaa aagctgaacc tattttcaat gatataagaa aatcggtgtt
    14521 gaataaatat gacagcggaa cagaaaaaat cactgctact caggcatggc aaaacgcaaa
    14581 agttttagcg aaaccagcta tcccttcgca gttttctttc tcgtcattag tagatacgct
    14641 tgctaatgtg aagatagaaa aggcgaattt tttagaattc tttggtggaa aagagggatc
    14701 tattctagat cgatttcatg gtgagtttct aaaagataat aacacattac gtcatgaaaa
    14761 aagtctaggt acggatcata aaatcaaagc gatttatact aaaaacttaa ctggtcttga
    14821 cttagagatt gatgctcaaa gtgttttagt tggagtttat ccattcatta gcagctcttc
    14881 agaaggagaa gacgagatca ctctaccaga agaagttatt ttcacggatt acgtggatga
    14941 ttatcctgct ggatatgttt cttttgttga ttttaaagac aaagcgactg atgtagccac
    15001 attaagggaa gctgctaaag actggttgaa aacaaacata gataaacaaa aaccacaagt
    15061 gagtggttcg attgaattag taccattgag gcatcaaaga ggctatgaaa aatttgttga
    15121 tctagaaaaa gtttcgatgg gtgacggagt agatgtgtat catccacagt taaaagtgaa
    15181 tatgtcagcg agaattgtgg aatatacgtt taatgttcta accaattcat acgataaatt
    15241 agttgtagga aacgtcaaaa caaacttctt agaaaacaca gagaataatg taagcaattt
    15301 gattaatgat tccattgatc aattgaaaaa tggtggcgaa atcagtgatt taatcaatga
    15361 tattgtagat catcaaactg atatgattac tggtcaaaat ggtggttatg ttttattaga
    15421 tcctaaagaa gcgcctagtc gtattttgat tatggacacg ccagataaga ataccgcacg
    15481 gaatgtttta caaatcaaca acgctggtat tggtttttct aaaactggta ttaatggaac
    15541 atatgaaacc gcatggacgt tagatggcgg attcaatgcc tcgtttatta cagctggtga
    15601 gatagtaggg attactatta gaggtactac attaattagt gatggtgctg attatagaac
    15661 aagtatagct aatggcaaaa tgacttggta ctcaaaaaaa gttaacaaag atattatgga
    15721 gctagaagca cgtgattatg taagtgctga tgccggtatt gtatcataca ccatgaaaac
    15781 tggtggtggt ttcatgatta gaaatccaca gggtaacttg gtttttagta cgtgggataa
    15841 tggtaataac agaccgtttc tatcttttgg tgcgcccaat ttcaggtata gcaatgctag
    15901 ttatgtaact tctggcgacg gtagttcttt aagcattaat ggtagtgcgg gtaactcatg
    15961 ggaatttaag gtagctggta ggactatgaa atttactagt gatggtatgc taacgttacc
    16021 aggttgtttt tttggttcat gggaagatgg gaaacttgct aggtttgaac aatcaacggt
    16081 acaagtatat aaagatttta ctgttagagg tactaaaaac tcaactgtac caacagaaca
    16141 ttatggacaa cgactattga acgcttatga aactccagaa tattatttcg ctgattatgg
    16201 ggaagccgtt acaggtgacg atggtaaagt tcgtgttgat attgacccca tgtttgctga
    16261 gacagtaaat ctaagtcggt atatgacaca tgtgacacct acagaactag ttttgtgtgc
    16321 tgttactcat gaagatattg accatttcat cattgaaact agtaagccaa acgtattagt
    16381 tagatggaat ttagtggcac accgtctagg gtatgaagat attagattaa aagaggatac
    16441 agcatatgat agcacagtgc ttgaccaaaa acgtttttaa aacgaagaca aggaggtata
    16501 taaatggcta gcagtttata taatttggct ttagatttca gcaaagaatt aaactacacc
    16561 aaagctatta tggctcgtca gggtgataaa gggattacgg tgacggttaa accatattta
    16621 aatggcttgc agatggatac gagtggcgga acatttactt taaaaggaac aacaccatct
    16681 aaccgttacg tagatagtgt tgcaactagc gtaactagtg aagaagtcac gttttctctt
    16741 gatggcacat ttatgagtga agcaggatat tataaacact gctacgtaga atatagaaaa
    16801 gacaatcaaa ttctaacaac gcaagatatc atttttttct cactaggagt gtctgacatt
    16861 tcgcaaggtc aagccgatga atatgtttcg caattagaag agttgattcg aaagtataat
    16921 gaaacttttg atgcttttat ggctgaaact aaaggtagag tggatagctt aaatcaacag
    16981 attactgatt taactggtca agctaaaacg ctacaagaca agttagatgc tctgaaagaa
    17041 gaaatttcta agttaggtaa cttacaagtg atgtacagta acagcatcaa ctttggtgac
    17101 tatgattata gtggaaatcc taatttgtta agtaagctat catacgactt aattgaaaat
    17161 caaaatactt cagctggaac actttctaaa ggtgaaaact cgtttaaata taataagata
    17221 tcagctgaag tggaaggtgg agtagagcta tattataaac gaagaggtat agctaactgg
    17281 ctaccatcta ataaaacgct tgtaatgact gttaagctta gagctggagc ggactatagt
    17341 ccagttgacg gaaaacttat actgattaga tataggtatg ttgacagtgg aactggcaag
    17401 attgttttag acttacctat taacagtaat tcgataactc aggaatggaa agagtttagt
    17461 attactggaa ctactccaac atttagccca caagcatacc atccttggat acaatttagg
    17521 gctcaagatg ggatacttgg ggaaatagaa atgagctatg acatcaaaat cgaagaaggc
    17581 tcaacagcta caccattcca acctaactta ttagcagaac cttacaacat gtgtcgcgaa
    17641 tatcctaacg aaaatattgc cgatcataca gttaagttcc caatcgaatc tggcgaccac
    17701 caaatatatc aaggttacac agaagaagag cttatgatag gtcaaacgta tactatcacg
    17761 cttaaaggaa caaaacccgc aagtcaaacc tttgtagcgt ataatcattg gactgctcgt
    17821 ttaggagaac taaagccagt tgatgggttg acagacgtat ggtctctaac attcacacca
    17881 acgaatgttg tggcgagttc acctaaactt tttcgtgttt atcagtatcc acaatcaaca
    17941 gtaggcgcat gccggatcga ctggctcaag attgaaaaag gcgacacacg aaccccgaat
    18001 attagtgagt ataaatatcg tggtactggt atgcgtgatt caaacaatcc aaaagattat
    18061 gtttgggatc tagcaccaga atatgtcgaa gataacttgg ccacagatat taaaatttct
    18121 gaaattactg gtaaagcaaa caattatacc gatgggaaag tatcggagat taattcgcag
    18181 ttgactgctt caattaatga agtagacacc acagctaagg atgctcaaac aaaagcgaat
    18241 gctaatgcga ctgctataga tgaattagac aataagatcg atgaacgcat taatgataca
    18301 gctactacca cattaacagt tacaaacggg aataccggat cagcaaagct ttatcgtgaa
    18361 ggaaaaacag tttctatata ttttgtggct ttaaacggaa aaagcagtgg tggaaatgat
    18421 tcaacgatac taacaattcc agaaggctat cggccaccaa ttagttttga gcaactggtt
    18481 ggctcgatag accgttctac tttgaacagt gctcagttat ctattggtgc agatggagcc
    18541 attaaatggc gaagaaactc aagttatgga tcggattata cctttgcaat tacttacacg
    18601 atttagaaaa gcgtgaatcg atatgaaggc agcatataga ccaattgaac cttacggatt
    18661 cgagcaaatc attgtgaatg atgaagaaca tttaccggaa gaatgcacag aagtcgaacc
    18721 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
    18781 tggaaatatg gacgtactgg cggagagtac gcaggaaaag tattggacga catgcttgta
    18841 tccgttcctt acacagatca gccaccgctt gaaggaattc gtgctgatgg cgaaccgtta
    18901 acaatcgctg atcaaatgtt tgatcctaaa ttgaatcaat ggattatttt agcgaacgca
    18961 ctagatcaca acgatttaaa caatctcaaa gcaatgtatg agtcgttaga aaatgagaac
    19021 ggcgatttaa aacagatcaa tgccaaactc atgctaagcg atgtagcaat taaacaggaa
    19081 aatactgcat tgaaagaaaa agcggatagt ttagcacaaa tcaattcaaa aatgatgctt
    19141 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
    19201 aagggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
    19261 tttcactaac gaacaagtaa tggttttcgt tccgttgtgc attactgaag aagaagcaga
    19321 taaaatcatt agcaaagaag agagcgcatc ttaattgatg tgctttttat tttgattcaa
    19381 ggagttgtca catgattaat ttaggggaat ggggagcgat agcaggatca ataaccgcta
    19441 tcgtttcttt gattttatta gtaataaaac caattactgc atctttctcg aagattactg
    19501 agactctttc aaaagtaagt cacaatttag atttgctgac taaagattta gaatcgagca
    19561 aatcagatcg attgatgatt catgaagaac taaagaaaca cgatgaaaga ttagatacac
    19621 atgcagaaaa attggtagaa cacacgcaac aaattaaaac tttatttagg gagagaagaa
    19681 aatgaataat aaaacgttcg aagtactaaa atggttcgca ctggtaatta ttcccgcact
    19741 agctactttc gtggggttag ttggtaaagc gctcaattgg cagtacacag atatctgtgt
    19801 tgtcatcatt actggttttg gcgcgttttt agggagtgtg ttgggtgtat caaatcgaac
    19861 ctacaaaatg ttctcggctg aaagcgaaga aggaggaaac aaatgaaaaa gaaaattact
    19921 attactgcga tgagcctgtt aacggctctt tttttattac ccattaatac gtttgcttat
    19981 actattaatg acgagtataa tttagcgccg aatcaaggag actccagatt agcaattcct
    20041 aacaaaatta ttttgcacga aactggaata gatgcaccag caagaaacgt agccgccaac
    20101 atgaaaaata attataacgg aagcaatcct tatactacag atgttattgg tgacggtggg
    20161 attgtttacc gtgtgggtga gcaaggatat gtttcgtggg gagctggtaa cgctaatcct
    20221 tatgcgcctg tacagattga attacagcgc acatatgata aagcattgtt tgaaaaaaac
    20281 tatcgagctt atattgaata tacaagagat agtgcaaaaa aatatggaat tccattgact
    20341 cttgatcaag gaacttcttt atttacaaaa ggaatcattt ctcatttgtg ggtgacaaat
    20401 tatgtttggg ggaaccacac agatccatat ggttacttat cgcaaatggg agttagcaaa
    20461 gaaaagcttg cttatgattt agctcatgga tttaccgatg aaaatccaac aacttctgaa
    20521 aacaagcctg tcattgatcc aacacgagct ggtgcagcta atcctacgct gacagatgga
    20581 acaaatcacg cccacattga tcagtttggg gaaatcgaaa a
//
LOCUS       DAEGIQ010000046        20494 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000046 DAEGIQ010000000
VERSION     DAEGIQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.046"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..494
                     /locus_tag="KMT45_001496"
     CDS             <1..494
                     /locus_tag="KMT45_001496"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004964.1"
                     /translation="RENKETTKEVQEDLKEYSASLNKVLSESVKKINEIFEDNYKDIG
                     SYLKVFHRILKILNNRYESKDISHEEYKMYVGILRSQIEDYEFYIILINSLFTLRGTG
                     MGIELIETSFFGDELDIDLKQHFEHPEFLTEIIKDNFVEKKSYRESVKTRKKIENYLK
                     IER"
     gene            623..1795
                     /locus_tag="KMT45_001497"
     CDS             623..1795
                     /locus_tag="KMT45_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBE7004965.1"
                     /translation="MSLFDIFKQSIKNEDPSDWIPDFVFGDEESARAYLKIMAKSTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWAMSDVIYMEYNNEELERFTR
                     GLFEDYAELFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDKIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIADVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1782..2486
                     /locus_tag="KMT45_001498"
     CDS             1782..2486
                     /locus_tag="KMT45_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBE7004966.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2491..3651
                     /locus_tag="KMT45_001499"
     CDS             2491..3651
                     /locus_tag="KMT45_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBE7004967.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTVEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVEYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3727..4005
                     /locus_tag="KMT45_001500"
     CDS             3727..4005
                     /locus_tag="KMT45_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBE7004968.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3986..4357
                     /locus_tag="KMT45_001501"
     CDS             3986..4357
                     /locus_tag="KMT45_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBE7004969.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4373..4777
                     /locus_tag="KMT45_001502"
     CDS             4373..4777
                     /locus_tag="KMT45_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004970.1"
                     /translation="MSNVKFEGVQETIQALEKKFGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIDEVVRKNATYRNYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSEPLYFDAVGEEVKKRL"
     gene            4774..5184
                     /locus_tag="KMT45_001503"
     CDS             4774..5184
                     /locus_tag="KMT45_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004971.1"
                     /translation="MKDMLNIIYEALCLNEYIRSMTYNENTEQYRIKFYEQPETADKT
                     GPMITLRPVDTPNEAYHGSNKELSVEHMVQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFSETKRFVDARRYEGNTKIYDTEY"
     gene            5245..5832
                     /locus_tag="KMT45_001504"
     CDS             5245..5832
                     /locus_tag="KMT45_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE7004972.1"
                     /translation="MTLVGFKKMTIGIFDSTGKIPAANLYVIEGEQDKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITALKEMVFPAAGE"
     gene            5905..6372
                     /locus_tag="KMT45_001505"
     CDS             5905..6372
                     /locus_tag="KMT45_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HBE7004973.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6418..6726
                     /locus_tag="KMT45_001506"
     CDS             6418..6726
                     /locus_tag="KMT45_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004974.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6921..13685
                     /locus_tag="KMT45_001507"
     CDS             6921..13685
                     /locus_tag="KMT45_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBE7004975.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGITGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRDLATKLPASHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKMLKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKALDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDNTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDIVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPNDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPNAKGTNFHPG
                     GAAMVNDQKGPTYEELITLPSGEAFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYADG
                     IGNITTVSSTLNLDALILAINELTTVLRTQLMAETTNAKDTKLSDETKVADPIIPGSL
                     SEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGVAW
                     NKNLLNGWNSLTGTFISTINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGS
                     FIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVG
                     GMGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEE
                     YVQMPDGRGFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLL
                     DDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDGA
                     VNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYT
                     AAANYAFEKFPSKIAYVITNGRVRDRMGLSGTGSSGQWTNWPDGDHFDHIHLNGSMGS
                     GDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGND
                     GLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGK
                     GHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGDDKKRSNTT
                     NTADNSAELVTLIKQQQKQHNELMMILRAILGKDLSLKSSDIGQAANHYMGSDLNKLR
                     YTNGGV"
     gene            13689..14510
                     /locus_tag="KMT45_001508"
     CDS             13689..14510
                     /locus_tag="KMT45_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBE7004976.1"
                     /translation="MFYKLQFNQNGKLFDPQRKEKIVCKEIKRQAPVYEVNYEDFEGT
                     NGSREANASFRPFELVLTFDIFYKNKHDKELLLTEFYELIFVGYQYYISYDLSPGKRF
                     KVNPTNFEITEEENDYSTIEITFSIPSGSAESSATTLTDFNLEEEWQFSQGLVAEDYK
                     YTHQTSHFIIYNAGSFEIDPREHYLRIALEGESEGNVTIFNKTTGDRFIYYPSLSTNL
                     GQTLVLDGVIPKLNGVSCGINTNHGLINLVEGVNEIEIQNITRVKSSWDFRFLYK"
     gene            14519..15562
                     /locus_tag="KMT45_001509"
     CDS             14519..15562
                     /locus_tag="KMT45_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE7004977.1"
                     /translation="MTELIIRNYEQTKEEILVGYDKGSFYENWQQNETWEIGFTVTSN
                     SLNQEVFDLVEYESSVFYNGQEFVIKEMTRKALGQLLTKQVVATHIYYTVQDGYQYNT
                     VTGAKSISQLLTHVFSAGSRGFTWEVIDPNKKFLTVEQENFGNANYLKLINEILSDYN
                     AVVIPNNKHLTFYPASEYGQQTEEQIRYKYNTDEVSFDIDTYSLKTQIKGYGKLKDGA
                     NTENPKDSDYIFTPITYTSPESQKWGIRIQDPVKDERYTVSGNMLERLKTDLQDYPNI
                     SGSVTLKWKISPNKGDHVPFIYEPLNINTYIQVVGIKTYPAIPNKPPEITLSNTKKTM
                     TSILAEMAKKGVI"
     gene            15562..16593
                     /locus_tag="KMT45_001510"
     CDS             15562..16593
                     /locus_tag="KMT45_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004978.1"
                     /translation="MGLLKLISNRISTEWKEKFNKNIDYLNDLEKKLSDQDKSMNSRI
                     DNLVLHSGGESPNEVVDARVNNKGETFPTLHGRLVEHETLTDEQISELNTNMDSQKEQ
                     VQQLNKSVQQIIGGYNEPINIYVSKDGSDILGDGSEEKPFLTIQTAVNNVPLITTSQI
                     TIWVGSGAYLEDIMIRNLNFTSFLIRPLDNFDTIDPLKSDLPVKVRSICFTACKGYCQ
                     VAGMQIVDTANGADYGIRNEQSGYMAINKCKFSENTKSLARYNAVYVGGTSKINMYGD
                     TTFINQKVAIYAVLMGEIFVSAFGSGNDIGILCENGTVRGTVSTSFATTPTKTTGYGL
                     IITKGTVLS"
     gene            16593..18695
                     /locus_tag="KMT45_001511"
     CDS             16593..18695
                     /locus_tag="KMT45_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBE7004979.1"
                     /translation="MVYKTNESIIMIQAEATSPNRTNVVFWSHDRGTAKLRMKLVRKN
                     GIPQSLPEGTTVPIRLMLKSATAEGGYGKHDYLATIEDRVNGIVSIVLEDNILGYVGI
                     VEGSVYIDFPNDRSLDTAGRFTFDIKRSPIDDSTPELEDYYFNGFSQTIDKIEKILAD
                     GKQEIEQKITESETQIDGKLKETSNKITKANQDVATINTNIDKANDRIDQTNQQISDL
                     GKLKKMYSNSIDFGNYDYSGNPNLMANINADSFSQGSGALSVVDDGDEVVITLDPNHK
                     LEIFKAKSQPALVVGKTYTMSVEIMLEDDFTGDPSNISLRYIKMPSWLTVLYTPYTLT
                     NTKGVWQKLTVTAKMTTAIDNAESWFIMLQNKDANNSLSGKLRLRHAKLEEGSTATPF
                     QPNLLAEPYNMCREYPNENIADPKVKFPIESGDHQIYQGYTEEELMIGQTYTITLKGT
                     KPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVAMPKLFRVYQYPRSTVGA
                     CQIDWLKIEKGNTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKGLNNAVNVYD
                     PQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATFIKHGKEVVVNG
                     TVKFKKAYAFGVPLDDEVPDEFIARIVHGMLTGQSGTNSVSKAMYVQKDLGKIMTNSE
                     FAANEWFTFHGNYWVGVK"
     gene            18696..19142
                     /locus_tag="KMT45_001512"
     CDS             18696..19142
                     /locus_tag="KMT45_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7004980.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGLRADGEPL
                     TIADQMFDPKLNQWIVLANTLDHNDLNNLKAMYEALEHENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPTLKGGE"
     gene            19144..19281
                     /locus_tag="KMT45_001513"
     CDS             19144..19281
                     /locus_tag="KMT45_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBE7004981.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            19316..19558
                     /locus_tag="KMT45_001514"
     CDS             19316..19558
                     /locus_tag="KMT45_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBE7004982.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            19574..19771
                     /locus_tag="KMT45_001515"
     CDS             19574..19771
                     /locus_tag="KMT45_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBE7004983.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            19782..>20494
                     /locus_tag="KMT45_001516"
     CDS             19782..>20494
                     /locus_tag="KMT45_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBE7004984.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIE"
BASE COUNT         7284 a         3280 c         4393 g         5537 t
ORIGIN      
        1 ttagagaaaa taaagaaact actaaagaag tacaggagga tttaaaagaa tatagtgcat
       61 cattgaataa agttttaagc gaaagcgtta aaaaaattaa tgaaatattt gaggataact
      121 acaaagacat aggtagttat ctaaaagtat tccataggat tcttaaaata ctaaataaca
      181 ggtatgaatc aaaagatatt tcgcatgaag aatacaaaat gtatgttggc atattacgta
      241 gtcagattga agattacgaa ttttatataa ttcttataaa ttctttgttt actcttcgcg
      301 gaacgggtat ggggattgag ttaattgaaa catcattctt tggtgatgaa ctagatattg
      361 atttaaaaca gcatttcgaa catccagagt ttttaactga aatcattaag gataattttg
      421 ttgaaaagaa atcgtatcgt gaatcagtaa aaacaagaaa aaaaatagaa aactatttga
      481 aaatagaaag ataaatgata acttattatc tcttttgaga ataagataca cgttacaaga
      541 tggaaataaa attattataa gtaatggagt atagtcctag aaatagggct ttttatttta
      601 ccttgaaagg tggtgaaaat atgtgagttt atttgatatc tttaagcaat caataaagaa
      661 cgaagatcca tcggattgga ttcctgactt tgtgtttggt gatgaagaat ctgctagagc
      721 atatttgaaa atcatggcta aaagtacagt tcttgatttt gttgcaagaa ctatgtcaac
      781 gttagaagtt aagtttaaaa ataaagatgg gacatcagat tgggattaca ttctaaatgt
      841 ccgacctaat tctgatatgt cagcagctac attttggcaa aaattttttt atcggctctt
      901 agatgacaac gaagtgttag tgatatttac agatgataat caattgctga ttgctgatga
      961 ttttagtcga acagagtatg ccgtgtatga tgatgttttt acaaatgtga tggtgaaaaa
     1021 ttttgtcttt gaacgttcat gggccatgtc tgacgtcatt tatatggaat ataataacga
     1081 agaattagaa aggtttacac gaggtttatt tgaagattac gccgagttgt tcggacggat
     1141 tctagaagtt gcaatgcgca ataaccaaat tcgtgggtct gtctccattg attcaacagg
     1201 aacggcaaat gatgaaaaag gaaaagatgg taagactaga tctcaaagat tacaagaata
     1261 tattgataag atttggaact cttttaaaac taactcagtt gcaattgttc ccaaagtgaa
     1321 aggctttgag tatgaggaat acaccaataa acaaggcgta tcgaatcaat cactagatga
     1381 actgaataag atgaagtcgt ctttaattgc cgatgttgcc aacgccatag gagtacctac
     1441 ggcgcttatt tatggcgaaa aagctgaact agattcaaat attaaagctt ttcgtaaatt
     1501 gtgtattgct ccactagtta aaaaacttca ggacgaattg gtggcaaaaa taattagtcg
     1561 acaagagtac aaaaacgggg aacgtataga agttaaaaaa gttctccaaa aagatctatt
     1621 agagtatgct actcaaattg acaaaataac atctggtggt gcgttttata ttgacgaagt
     1681 tcgtgatgaa gttgattatg atgagttgcc aaataatgaa ggaaaaattc gtattcgtac
     1741 gaaaaactat gaagaaactg tgaagggagg tgagaacgac aatgccgaaa gctaaaaaag
     1801 taccatttca gtttactaac gaaattcaga atggtaagca cattctcacc ttgagtggaa
     1861 atgtacagaa aaaatattgg cgtgatgatg atgtcataaa cgctaaagat attcgtgact
     1921 ctctagatac tgtaaccgat gatattgtaa tcaagttgaa cagcccaggc ggtgatgtgt
     1981 ttgaagggat tgagatttac aattatttaa aagatcaccc atcaaatatt actgtagaag
     2041 ttacagggct tgccgcaagc gcagcaacgt ttattgtcgc tggtgccaat gatgtgatta
     2101 tgaatgttgg gacttctttg atgattcatg aagcttctac atttgcttgg gggaataaac
     2161 aggacatcca aaaaacgtta aacgcattgg aaacgatcga tgattctatc ttagctattt
     2221 attcagataa gactggccaa tctgttgata agttgcgtga atggatgaat gaagaaaaat
     2281 ggtttacagc agatgaagct gtcaaatatg gatttgccaa ttctgtgaag cgtgaagaac
     2341 cacaaaacga acctcaaaat attgcagcga tgattcaaga tgctgttgcc gttgcaatag
     2401 cgaatttaaa ggaacctgtt actaataaaa tcgaacaaga atcaaaacaa aaatcattaa
     2461 tcgcacgatt gcgaaaagga gaataaagat atgttaaaaa ttacagataa aactgcagat
     2521 gcaaaaaagg catttaatgc tatttcttcg aaagaagatg caacacccga acaaattaat
     2581 gctgctctag aagaatatgt aactgccatt gcagaagatg ccggcaaaca ggtgcgtgct
     2641 gaatatgaag aattgaaaaa tgtgacggac aatcgtgtat tagaggctcg tggaattcct
     2701 acactaacag tagaagaaac aaagttttat aatgaagccg taaaatctgg aggtttcgat
     2761 tctgacgtag tttggccaga aacgattttg gaacgtgttt ttgaaaatct tcaaaacgat
     2821 catcctattt taggcctcat taactttaca ccaactgtag gtcgtgtcaa agtaattaga
     2881 gcacgtcgta aaggtgttgc tgtttttgga ccattgcata aagaccttga aggccaacta
     2941 gatgctgaat ttggtgcaac tgaatttgtt caacttgcgt tgaccgcatt tttcttaatt
     3001 tctaatgata ccttagattt aggaccacga tggattgacc gttttatcaa tctttcttta
     3061 agtgaagcag tacgtgatgt ttgggctaaa aaaattatta caggttctgg taaagatgag
     3121 ccaattggat tgcttaaaga tctagatggt gcggtcacaa gtggtgtata tccagataaa
     3181 gctagcgcag ggacattaac ttttgcgaaa gagaagattg ttcttgaact tgcaggggtg
     3241 atgaaaaaat tatctaaata cacatacaaa gtagatggtg acgatgaagg tgaagtggaa
     3301 taccgttctg ttgctggacg agtgtatcta attgttaatc ctgttgatta ctgggatatc
     3361 gtagctagag ttacatttgc aaatttaaac aatgtgtatg ggactacgat gccatttatc
     3421 gctgttgatc atatcatcga atctgtagac gtaccagaaa gtaaatgcat tgcatacgtt
     3481 gataaagagt atgaagctac tcaatcaaga gctgaaaaag tctatgtata taaagaaact
     3541 tttgcaatga aacgcgcaac tttgtatgct gtcgacatgc ttggtaatgg ctatccaaca
     3601 aataatgatg cagcacaagt atatgacttg gactttacag cgggggagta acgtcccctg
     3661 caattggtga gataacaccg acgactgatg gggcagtcat taatctttcg taaatggggt
     3721 tagagtatga acactgaaga atttataaaa gaatataagg cccgatttcg tattttccat
     3781 acttccgaag atgaggatat cgggaaacag ttagagagcg ggtttgccga tataaaatct
     3841 ctaatcggca gttttgatcc ttccacttat gagaaaggaa aagaattagt ttatgaacgt
     3901 actcggtatc tacggaacga atcactagag tacttttacg acaattttca aacaatgatt
     3961 atggatgcat caattgatct ggcaggtgct ggatatgcca attaaatcaa attataagaa
     4021 gcctaaaatt gtagctggtg atctaaatac gccagttact ttttttgagt ttcaacctaa
     4081 taagggtcca gaacctggtg atgaagaaaa aaaggaactg tatttttgta cagcactagt
     4141 ttacaaccca tcaatgaaag atcgtgacat tcttggcaca aaagaaggcg ttacgataaa
     4201 aatacgggat ccccatcaat cttatattcc tactaacaaa cataaagtaa ggatattaga
     4261 ttatcgatat ggagataaag tttgggaaat aatcgatgtc tcaccggatt tggaagaaaa
     4321 ttcaattgta aagatagtat tagaattaat tacttaaatt tggtgataaa cgatgagtaa
     4381 cgtaaagttt gaaggcgttc aagaaacaat ccaagcttta gaaaaaaagt ttggagaaaa
     4441 gaaaactaag acattaacaa aaaaagcaat caatgttggc gctgaaaaag tagaaaaaca
     4501 gttgcaaacg gacatgaccg tttttaaaga caaaggttat acgattgatg aggttgtacg
     4561 taagaacgct acgtatagaa attacaaggc agaagctgaa atcggttgga atggtcctca
     4621 tcagcgttat agaatcattc acttaaatga atggggatat acacgaaaag gtaagcaaat
     4681 taggccaaga ggttttggcg taatcactaa atcgcttaaa aattccgagc cactatattt
     4741 tgatgctgtt ggggaggagg ttaaaaagag attatgaaag atatgctcaa tatcatttat
     4801 gaggctcttt gtttgaatga gtatattcgt tcaatgacat ataacgagaa taccgagcaa
     4861 tatcgtatta aattttatga gcaacctgaa accgccgata aaacagggcc aatgatcaca
     4921 cttcgtcctg tagatacacc taacgaagca tatcacggaa gtaacaaaga gctttctgta
     4981 gagcatatgg tacagattga tgttgaatcg aaatatagag caacatgcaa gcagattcaa
     5041 tatgagataa aaaaagagat gaaaaaacta ggctttggcc aaatttctgg tcaagggtta
     5101 gatgaatact tttctgaaac aaaacgcttt gtagacgcgc gtcgttacga aggaaataca
     5161 aaaatttacg ataccgaata ttagaaatta agacacgaaa ataacgtgtc tttttttatt
     5221 acccaaaatt aggaggaaaa ttttatgact ttagtaggat tcaaaaaaat gacaatcggg
     5281 atctttgaca gcacagggaa gattccagca gcaaatttat atgtaattga aggtgaacaa
     5341 gacaaagggg cgactgtatc tgctgaaatc agtgggctat caaaagaacc atcaaaagtt
     5401 tacggatcga acatcgctta ctatgtctct caaaaaggaa caggcgatgt ttcagctact
     5461 tttggattgt tagatttacc aacagaagta aatgacaaaa ttcttggtta caaaaccgat
     5521 acaaacaaaa tcagcttctt gggagaagat actgagccac catattgtgc aattttgatg
     5581 gaatcggagg atttgaacgg cgatacagca atgcttgcga tgttcaaagg aaaattcagt
     5641 cgtgaatcta ttaatcttaa cactacgaca aatgaagctt tcgaaccaga agcagaagaa
     5701 tatgtatttt cagcgattgc caatgatgtt gaaggtgatg ctaaaggcca aacagtcgca
     5761 aaatatgttg gagacgaaga agcttctatt actgctttga aagagatggt atttcctgca
     5821 gcgggggagt aactagctcc gttgttggag caatcactcc cactattaat ggggtaacag
     5881 ttgaattaag ttaggaggat aactatgcca gatacattta aaatctataa aaaaaatggt
     5941 actgaattta caaaagtcgc agagggtgaa agtccacttt ctattactgg aattgcagca
     6001 aatactcaag ttgctaaagg agactaccaa gctacgcgtg tggttggagg tcaagaatca
     6061 gtaaaagtag atattcctgc atttaccact cttccaattt cagttactgg tgtgacgttg
     6121 gataaaacga cagctgaagt cgaacaaggc ggaaccttaa aattaactcc tactgtaaca
     6181 ccagcaaatg caacagacaa aacaggaagc tggagcagtt cgaatacagc tgtagctact
     6241 gttactggag gcaacgttac agtaaaaaca gacgctgaag ttggcggaac tacagaagtt
     6301 tcatttacta ctacggatgg cggtaaagta gcaaaatgta ctattacagt tattgaaaaa
     6361 gcagcgggct agtcttcgga ctagcttttt tatttggaaa ataatggagg aatctaaatg
     6421 gcaaaagtaa gaattgaatt gaaagaagaa tcaggaaaga aagtctatga aaacatagac
     6481 acaacaggta aagattatcg aaaagcttta gaaacaatca aaaagctaaa tgaagatgga
     6541 gtaatgattt ggaatcgttt agacatttat ttggattttg ctgtaggtat cttcagggat
     6601 agcaaattga cagctgaaca aattcttgat ggcttaccat ctgagaaggt aatgagcaca
     6661 ttggacgaca tcctaggaga agtaatgggg attgaaagta atccagaccc agacgcaaaa
     6721 aagtaacacc cgaagaagca gaagaaatgt acttaaacct atgcagagag ttagtgaaag
     6781 cgggatggtc actatctgac atagaagata attcctttga aatgatgatg aaaattgcgt
     6841 gcacttcgcc taagaaagag aaacaaaaag aagttgatct taaagacttc ttgaaatcca
     6901 tgtagagagg aggtaaacat atggcaaatg gaaaaccttt aggcaatatg attataaaac
     6961 tagacttaga cagttcagct ttttcaaaag gtctagccgg agctaaaaac gctgttaacc
     7021 atcaaatgaa agcaatgaaa tcacagatgc aagtaatgaa ctcttcaggg aattctctcg
     7081 gtgctttaca agcaaaatat aatggtttag gcggagttct acaagcaaat gaaaagcaag
     7141 tagaattgct cactaaggct tataaggata gttttgactc taatggaaac gctactgctt
     7201 cgactgctaa atacgctaat caattgaatc aagcaacagc tagatcagca agttacgaat
     7261 ctcagatgaa aacaaccgtt ggacagattg ctcggatgaa agtggaaacg gaagggatta
     7321 cggggaaact aaaagctaat tctgatgtat tgattaattc tgggaaaaaa attgaagcct
     7381 ttggtaaagg agtttctggg ataggatctg ccctaactgt tggagtaaca gctccaatag
     7441 tggcaggtgc aactgctgtt actagtgcag ctatttcttg ggaaagtgct tttgcaggag
     7501 taaaaaagac aaatgatgaa gttgtagatt cgaatgggaa cgtagtttac tcctacagtg
     7561 atttagaaaa aggccttcgt gatttagcga ctaaattgcc agcaagccac caagaaatag
     7621 cgaacgttgc tgaagcagca ggtcaattag ggattaagac agaaaatgta gttagtttca
     7681 ccaagacaat gattgatatg ggcgaatcta ctaatatgtc agcagaaaca gctgcaactt
     7741 ctttagcacg atttgctaat ataacccaaa tgtctcaaaa agactttgat aaattaggat
     7801 cagtcattgt tgatctaggt aacaattttg ctacaaccga atcggaaatc actgaaatgg
     7861 gactgcggtt agcgggtgct ggtaaacaga ttggaatgag ccaaggcgaa attctaggat
     7921 ttgctactgc tctatcctct gttggtgtcg aagctgaagc aggtgggtct gccttttcga
     7981 aggtcatgat ccagatgcaa ctagctgtag aaaaaggatt tggcgctttt gatcagctga
     8041 aacagatggc agaagaacaa ggtgttccgt gggttaattt agtaaatgct gtgagagacg
     8101 gtggaaaatc gttaaaagca gtatcagagc aaatgggatt cacttcatct gatttaaaga
     8161 aaatgtataa agaagcggac aattcgaaaa gttcgcttga aaattttgcg aatgtggcag
     8221 gtgttacaag tgaacaattc cagaagttgt tcaagagtga tccttcagaa gcaatcatca
     8281 agttcattca aggactgaaa gattcagaaa aacatgggac ttcagcaatc aaagttctgg
     8341 atgacatgga tataaaagaa gttcgactac gcgacagttt attgagagct gcaaatgcta
     8401 gtgacatatt caaaggagca attgaaaaag gaaacggcgc gtggaaacaa aatacagctt
     8461 taacaaatga agctaataaa agatacgaaa caactgaatc aaagctaaag atgttgaaaa
     8521 acgaagtgat tgatacagca atcgatcttg gaggtccatt tgtagacgcg ttgcgtgatg
     8581 gtctaaatgt atcaaaaccg ttgataaaaa acttagggaa tatggcgaaa gctttctcag
     8641 ccatgagtga tgatcaacaa agaaacatta tcaaatgggt tggattagca gcagcagctg
     8701 gtccagcgct gaagctttta ggaactggta cttcagtaat aggtaaagtt acttcaacta
     8761 ctggaagatt gacgaaaagc cttgttgatt tagcagcgaa agcagcagaa aagaaggctc
     8821 tggatgcctt tgcatcgtcg gtaacaacag ctggtgctgc agctgcaaag acagcaggag
     8881 ctggaggtat tggcgggtta gtccctatga tgggaaaaac agctagtgct gcaagtactg
     8941 ctgcgggcgc tagcggaata ggcgcaatga caacatcact aggtttgtta ggtcctgctc
     9001 tacttggtat tgttggcgtt ggtggcgcac tagcagttgg ctatggagca tggaaattat
     9061 ttggtgaaga agcttggaat tcctcgcaaa gagtaaagca atggggaaca gatgtcggtg
     9121 aggaaataga taatactctt gacggagtac agaagaatat tgaaggtgca aatggtcaat
     9181 tcggtcttct caaagagggt tttactgttg atgatgcgaa taatatggcg gctaatttcg
     9241 caaccgctgg aacatctttg gaaacatctt taacaggtag aataaaggca ttagacgatg
     9301 cactaaaagg gctacctcaa tcagttcagg atgccatgaa agaagtagct gaatcagaag
     9361 aagaaacctt agggcaatct ctttctaaaa taaaagacaa caatgaagaa ataaaaaaaa
     9421 tcagagaaag agccgcaaat cagaacagag acttgactgt ttcagaacta caacagatac
     9481 aggatctgat gaaaggaact tctcaagcgt atgtggatac gctcgatgtt tctgctaaag
     9541 acagaaaaaa gatactatca gccatgaatg gtgatgttgc acaggctaca gaagaagaag
     9601 cgaagacgtg gttacaatct ttagcgaaac aaaaagctgc aactaccgaa aattatagac
     9661 aaatgcttga agaaaaaaag gcggctttaa aggaaaaggg agtaagcaaa gaattcattg
     9721 atgcattaga agatgaaatg aatgattaca tcaaaactac aaatcaagga ttcgatagcc
     9781 aaatagctgc tataacaaat aaatatccac aattgattaa cgaagtgagt ttatcaaatg
     9841 gtcaattatt tagcaaaacg gcagctgcta tggacaatac tggtcagtat acaaaagttt
     9901 ttctcgaaag taatgaaaag attttagccc aagcgaagaa aacatcggat gaaatagcag
     9961 ataatgccga aaaaaatgca gaacaacttg gtatggtagc tgatgaggct acgcatgctg
    10021 gatcaacttg gaatggatta gttttagacg aaaaaacagg aaaagtaaaa actaacgtac
    10081 aagaagtagt gaatgaagca acgaaggatt tcactaaatg gaatgaactt aaacctgttg
    10141 ttcatgatgc gaaactaaca acgaacgcaa aagatgtcat ttccatagcg gcagtacaaa
    10201 atggttattg ggatgatatg gaatggaaag aaaaagaagc attattagaa gatgagtgtt
    10261 ctaaaaatgt atacaaggct ttagaaaatg ctggtaaatg gaatgagtta gatattcctt
    10321 ctaaagaagc aatcctaact tcaaatactc ctgaagttat gggtgaaact ctcatgaatt
    10381 tgggactatg ggatacttac aaacttcaag tgaaagattt agatttgaat gcgtataaag
    10441 tatacgatgc tatcgatgga tcagaagaaa aattaaatag ttgggctcaa ttagataacc
    10501 ccacaaaaga attgttatta gataactcag atttttctac aaaacttttt aattccgaag
    10561 aaatgtttaa acgttggaat cagttatcat ctaatgcaaa gtttctttca gctaataata
    10621 ctaaatttac agaggttctt ttatcatctg acagaatgtg gaatcaatgg gtaaaccttc
    10681 ctgataatga gaaaagatta cttgcagata acgaagattt agctaccact gttttatctt
    10741 cagaagaaat atacggaaga tggactcaat taccagataa tcaaaaatat atgttagctg
    10801 acaatactga cttagcaaac aaaatatttg cttcagaaga atcatggaat gcatggaaag
    10861 caattccaga catagttaaa aacttgcttg gtaataatat tgatttactg gctaaagtga
    10921 aagatgggac tgtaagtgta gaagaatata acaaagttat gccacttctt aaaactctat
    10981 atggagataa cgttaattta aaagaggcaa tcagtggggc taaaagctct atagagaatt
    11041 acaacaaaaa tatttttcct aatgataaaa ctgcagtagg acacgatgag gcttcacaag
    11101 ctgctaaaga tgcatttgaa gcgttcaaca tcttccaaac aaaaatacca gataaaatta
    11161 ctaaaactgt atcagctgac tttataggtc ctatgcctaa tgctaaagga acgaacttcc
    11221 atcctggcgg agcagctatg gtaaacgatc aaaaaggacc tacttatgaa gagcttataa
    11281 ccttgccaag cggagaagct tttataccta aaggacgtaa tgtcgtttta gacttaccaa
    11341 gaggttcgaa agtactgaac gccactaaaa ctaaacgtct agtacctaaa tatgctgatg
    11401 gtataggaaa cataacgacg gtttcatcta cgctaaactt agatgcttta attttagcta
    11461 ttaacgagct aacgacggta ttgagaactc aactgatggc agaaacgact aatgctaaag
    11521 atactaaact atcagatgaa acaaaagttg ccgatccgat tattccgggc agtctttcag
    11581 aaaaatcaga tcagtatcta ggaattggag ctcaatggtt aaccaatcta atgaatggtt
    11641 ggaactcagc ggttcctcaa tatatgaata gtgaaatggt ttttattaca aactatctca
    11701 atgcgctaaa acttcaaaac aatcctaatt atacgcaagg agtagcatgg aataagaact
    11761 tactgaatgg atggaatagt ttaactggga catttatttc tacgatcaat tcattttgta
    11821 atcaagcaat ggtcacgctt agaaactaca acactcctat gtacaacaat ggccgaacgt
    11881 ggcagcaaaa taatcttaat ggttggaatt cattgtatgg atcattcata gcccgtgtaa
    11941 atcagcttgg taacgattcg attaacaacc ttcgttctaa aagtggtggt ttttacaatg
    12001 cagggacatt cttgttgcaa tctttaataa acggaatgaa ttcgttaggt aattctcttt
    12061 ctacaacaat gaacaatgtc gctaatacaa tggttggtgg tatgggcaaa ggggttaatg
    12121 gcgtcatatc gggagttaac tatgtgctta aggaagtcga gtcaagtaag aatatcggaa
    12181 actggccaat tccacaatac gctaagggaa ctgatggaca tcctggtgga cttgctatga
    12241 ttaatgatca aaaaggccta gttcacgaag aatatgtaca gatgccagat ggacgtggtt
    12301 ttattgccaa aggtagagac cttcttgtta atttgcctaa aggtgctcag gtcttaaatg
    12361 cttctttaac aaagaaatta aaggaacgtt taaatattcc acactacgaa aaaggtattg
    12421 gcaatttaga tattgttgat ttgttagatg atgaaaaaag aatgttagaa ttcttgacta
    12481 gcaaagttga cttttcaggc attaatgaac catggcttga tatgactaaa tcaggaacgt
    12541 cattaatgtc taaagctgca aatacaatgc ttcaatcaaa attaagtgaa ttcttcactc
    12601 atggcaattt tgatggtgca gtaaatgcca atggtgttta tcaatattta gttgatgttg
    12661 cacagaaagt aatgggtaag ttccccggac ttacagtaac ttctggatat cgagcaggag
    12721 atgcttacta tcacggaaaa cggcaagcta tagacttggc ttatccaggt atatctggag
    12781 atccgagata tacagcagca gctaattacg cttttgaaaa attcccttca aaaattgcgt
    12841 atgtcattac gaatggacgt gtacgtgacc gcatgggatt atctggaaca gggtcaagcg
    12901 gacaatggac gaattggcca gatggcgatc acttcgatca tattcattta aacggttcga
    12961 tggggtctgg cgatatattc aatggaggag ttggaggaag tggtgttgca cgctggcgtt
    13021 cttatgtgtc taaagcattg aaaatgaatg gattaccagc tacagcagct tatataaatg
    13081 cctggatgtc ccaaatccaa acggaatctg gtggtaatga gaaagcaatc ggcggcaatg
    13141 atgggctcgc tgaaggcaat gcaacaggat tacttcaaac gaaacccgga acttttgcag
    13201 ctaacgcttt tcctggtcac ggaaatatta tgaacggatt tgataacatg ttagcagcaa
    13261 tcaattatgc taagaaacgt tacggagttg caggtatgtt gcaagttatc gggaaaggac
    13321 atggatatgc caatggtgga ttgattacca aagacggact atatcgagcg ggagaaggaa
    13381 ataaaccaga aatggttatt ccattgacaa gaaaaacaag agctattgaa ttgatgggtc
    13441 aagcattagc ttttctttct ggcgatgaca agaaacgctc caacactaca aatacagctg
    13501 ataattcagc agagcttgta actttgatta aacagcaaca aaaacagcat aatgagttaa
    13561 tgatgattct tagagcgatt cttggaaaag atttaagtct caagtcttct gacattgggc
    13621 aagctgctaa tcattatatg ggctctgatt tgaataaact tcgatatacg aatggaggtg
    13681 tttgataatt gttttacaaa ttacagttca atcaaaatgg gaagttgttt gatccgcaaa
    13741 gaaaagaaaa aattgtatgt aaagaaatca aacgtcaagc accagtgtat gaagtgaatt
    13801 acgaagattt tgaagggacg aacggtagca gagaagctaa tgctagtttt cgtccttttg
    13861 aattagtgct gacttttgat atcttttaca aaaataagca tgataaagaa ctgctattaa
    13921 cagagttcta tgagctaatt tttgtcggtt atcaatatta catttcctat gatttaagcc
    13981 caggaaaaag atttaaagta aatccaacaa atttcgaaat tactgaggaa gagaacgatt
    14041 attctacgat agaaataacc tttagtattc catcagggag tgctgaatcc agcgcaacca
    14101 ctttaacgga tttcaatcta gaggaagaat ggcaattctc gcaaggtcta gtggcggaag
    14161 attacaagta tacgcatcag actagtcact tcattattta caatgctgga agctttgaaa
    14221 ttgatccacg tgaacattat ctacggatcg cattagaagg agaatcagaa ggaaatgtga
    14281 cgattttcaa taaaacaact ggcgatcgat ttatttacta tccatcactt tctacgaatc
    14341 tcggacagac attagtcttg gatggcgtga ttccaaaatt gaacggtgta agttgtggaa
    14401 tcaatacgaa tcacggtctg atcaatttgg ttgagggagt caatgaaatc gagatccaaa
    14461 atattactcg cgtgaaatct tcatgggatt tccgtttctt atataagtag gtgattgagt
    14521 gactgagtta attattcgaa attatgaaca aaccaaagaa gaaatccttg tcggttatga
    14581 caagggttct ttttatgaaa actggcaaca aaatgaaacg tgggagattg gctttaccgt
    14641 taccagcaat tcgttgaatc aagaagtatt tgatttagtc gaatacgagt cttctgtttt
    14701 ctacaacgga caggaatttg tgatcaaaga aatgactcgc aaagcacttg gacaattgct
    14761 gacgaaacaa gtagttgcga cacatatcta ttacaccgtt caagatggtt atcagtacaa
    14821 cacagtgaca ggtgcgaaat ctattagcca gttactcacg catgtattta gtgcagggag
    14881 tcgcggcttt acatgggaag tcattgatcc aaacaaaaaa ttccttaccg ttgaacaaga
    14941 aaatttcggt aacgcgaatt acttgaagct gatcaatgag attctatctg actataatgc
    15001 agtcgtgatt ccgaataata aacatctaac tttctatcct gccagtgagt acggccagca
    15061 gacggaagaa cagattcgct ataaatacaa tacagatgaa gtgtcgtttg atattgatac
    15121 gtattccttg aagacgcaga tcaaaggcta tggaaaattg aaggatggtg caaatactga
    15181 gaatcctaaa gatagtgact atatatttac tcccatcact tatacaagcc ctgaatcaca
    15241 gaagtgggga atcaggatac aagatcctgt taaagacgaa cggtataccg tatcagggaa
    15301 catgctcgag cggttaaaga cagacttgca agactatcca aatatttcgg gatccgtaac
    15361 cttgaaatgg aaaatcagtc ccaacaaagg cgatcacgtc ccattcattt atgaaccttt
    15421 gaatattaat acgtacattc aagtggtagg aatcaagacg tatccagcga taccaaataa
    15481 gccaccagaa atcacattga gcaacacaaa gaaaacaatg acgtcgatac tcgctgaaat
    15541 ggcgaagaaa ggagtgattt gatggggtta ttaaaattaa tcagtaaccg tatctctacg
    15601 gaatggaaag agaaatttaa taaaaacatt gactacctta atgatcttga aaagaaactg
    15661 tctgatcaag acaaatcaat gaacagtcgt attgataatc tcgtgcttca ttcaggtggc
    15721 gaatcgccga atgaagtggt tgatgctcgt gtaaataata agggagaaac ctttcctact
    15781 ttacacggca gattggtaga acacgaaacc ctgacagatg aacaaattag cgaactgaat
    15841 acaaacatgg atagtcaaaa agagcaagtt caacaattga acaagtctgt tcaacaaatt
    15901 attggtggat ataatgagcc aattaacatc tatgtttcta aagatggtag tgatattttg
    15961 ggtgatggat cagaagaaaa gcccttctta accattcaaa cagccgtaaa taatgttcct
    16021 ctaattacaa cgtctcaaat tactatttgg gtaggtagtg gggcttactt agaagatatt
    16081 atgattagaa atttaaattt tacatcattt ttaattcgtc cactcgataa ttttgacact
    16141 attgatccat taaaatcaga tttgcccgtt aaggtacgat caatttgttt tacggcatgt
    16201 aaaggctatt gtcaagttgc tggaatgcaa attgtggata cagcaaacgg agcagactat
    16261 ggaatcagaa atgagcaaag tggctacatg gcaattaaca agtgtaaatt ctcagagaat
    16321 acaaaatcac ttgcaaggta taatgccgtt tatgtaggcg gaacatcaaa gattaatatg
    16381 tacggagaca cgacgtttat taaccagaaa gtggcaattt acgctgtatt gatgggcgaa
    16441 atttttgtca gtgcgtttgg ttcagggaat gacataggaa ttttatgtga gaacggaact
    16501 gttagaggaa ctgtatcaac ttcttttgcc acaaccccaa caaaaactac tgggtacggt
    16561 ctcatcatca ctaaagggac ggtgttgtct taatggtata caaaacaaat gaatcgatca
    16621 tcatgattca agcagaagcc actagtccaa acaggacgaa tgttgttttt tggtcgcatg
    16681 atcgaggaac agctaagctt cgaatgaaat tagttcggaa aaacggcatt cctcaaagct
    16741 tacccgaagg cacaactgtt ccgattcgct tgatgctcaa atctgcaacg gcagaaggtg
    16801 gttatggaaa acatgactat ctcgccacca ttgaagatcg tgtaaatggt attgtgtcca
    16861 ttgtgctgga agataatatt ttaggctatg ttggaattgt cgaaggtagt gtttatattg
    16921 atttcccaaa cgaccgctcg ttagatacag ctggccgttt tacttttgac atcaaacgca
    16981 gtccaatcga tgatagtaca ccagaattgg aagattatta tttcaatggt ttcagtcaga
    17041 ctattgataa aatcgaaaaa attctagctg atggaaagca agagattgaa cagaaaatta
    17101 cggaatctga aacgcagatt gatgggaaac tgaaagaaac aagcaacaaa atcacgaaag
    17161 ccaatcaaga tgtcgcaact atcaatacga atattgataa agcaaatgat cgtattgatc
    17221 aaacgaacca gcaaataagt gatctcggaa agctgaaaaa gatgtactcc aacagcatcg
    17281 atttcgggaa ctatgattat agtggaaatc cgaatttgat ggctaatata aacgctgata
    17341 gcttctcgca aggtagtggt gccttatctg ttgtagatga tggtgacgag gtagtgatta
    17401 cgcttgatcc aaaccataaa ttagaaatat tcaaagcgaa aagtcaacca gctctggtag
    17461 tggggaaaac atataccatg agtgtagaaa ttatgttaga agatgatttc actggagacc
    17521 ctagtaatat atctctaaga tatattaaaa tgcccagctg gttaacagta ctatatacgc
    17581 cttatacatt aactaatact aagggtgtat ggcaaaaact aactgttacc gctaaaatga
    17641 ctactgcgat tgataacgct gaaagctggt ttataatgct acaaaataaa gacgctaata
    17701 acagcctatc cggcaaacta cgtttgagac acgctaaact cgaagaaggc tcaacagcta
    17761 caccattcca acctaactta ttagcagaac cttacaacat gtgtcgcgaa tatcctaacg
    17821 aaaatattgc cgatcctaaa gttaagttcc caatcgaatc tggcgaccac caaatatatc
    17881 aaggctacac agaagaagag cttatgatag gtcaaacgta tactatcacg cttaaaggaa
    17941 caaaacccgc aagtcaaacc tttgtagcgt ataatcattg gactgctcgt ttaggagaac
    18001 taaagccggt tgatgggttg acagacgtat ggtctctaac attcacacca acgaatgttg
    18061 tggcgatgcc taaacttttc cgtgtttatc agtatccacg atcaacagta ggcgcatgcc
    18121 aaattgactg gctcaagatt gaaaaaggca acacccgaac gcccaatatt agtcagttta
    18181 aatacttcgg tgaaggattg aaagacagca acaatccgaa tgattacagt tgggatgtca
    18241 cacctgaata tactgaaaaa ggcttgaata atgctgttaa tgtgtatgat cctcagagag
    18301 ttgaaggttt gaaaaacttt gccgatggta ttcaaatagc aggagataaa gtgatcagtg
    18361 aaaatgattg cactgtttat acattaacta aagacaacag tcaatcgttt atcgatgggt
    18421 atgcaacatt tattaaacac ggaaaagaag ttgttgtaaa tggtacagta aagttcaaaa
    18481 aagcttatgc ttttggtgta ccacttgatg atgaagtccc agatgaattt attgcaagaa
    18541 ttgttcatgg catgcttaca ggtcagtcag gaacaaatag tgtatcaaaa gcgatgtacg
    18601 tgcaaaaaga cttaggaaaa attatgacga atagcgaatt tgccgcaaat gaatggttta
    18661 cattccacgg taattattgg gtaggagtga aataaatgaa aaacatttgg aaatacggac
    18721 gtactggtgg agagtacgca ggaaaagtat tggacgacat gcttgtatcc gttccttaca
    18781 cggatcagcc accgctcgaa ggattacgtg ctgatggcga accgctaacg attgctgatc
    18841 agatgtttga tcctaaactg aaccaatgga ttgttttagc gaacacactg gatcacaacg
    18901 atttaaacaa tctcaaagcg atgtacgagg cactggaaca cgagaacggc gatttaaaac
    18961 agctcaacgc caaactcatg ctaagcgatg tagcaattaa acaggaaaat actgcattga
    19021 aagaaaaagc ggatagttta gcacaaatca attcaaaaat gatgcttgct tcgttacaaa
    19081 atagcaaaga catttcagaa attaaagagc aactaaatcc aactttaaag ggaggtgagt
    19141 agtatgttta gttttagcga tgtgaaaatg atgtatgatt ggggctgttt tactgacgat
    19201 caagttcgac tattcgttcc actatgcatt acagacgaag aagcagataa aatcattagc
    19261 aaagaagaga gcgcatctta agtgatgcgt ttttttgttg gaaagttggt ggaacatgaa
    19321 agaagaagcg ctccaagacg ttgtggagag attagtaaga attgaaacaa aattagacaa
    19381 ctacgaatca cttagagaaa aggctgatag tgcaaaagat ttagcagata aagcctattc
    19441 agtagcacta aacaatgcag aagacatcaa ggaaatgaag aacaataata aatgggcttg
    19501 gggctatatg attggcttag gcattacgat cattggctat ttcttgacta aattgtaaag
    19561 gaggtgagaa gaaatgattt taccagataa gtattatcaa gtcattaaat ggacagtttt
    19621 aacagtattg ccagctgcat ctgctttagt agccatgtta gggaaagcgt atggatggaa
    19681 tggaacagat atgatagtgt tgactatcaa tgcagtagca acatttttag gcgttatcac
    19741 tggtgtgtcg gcttataatt tgaaaaaata ggaggaaaca aatgaaaaag aaaattacta
    19801 ttactgcgat gagcctatta acggctcttt ttttattgcc aattaatggg tttgcctata
    19861 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaaata gcaaataatc
    19921 agtacatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
    19981 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
    20041 tttatcaagt cggtcaacct ggttatgtac agtacggtgc tggttcgtat gctaatgcca
    20101 acagtcctgt gcagattgag ttacaacaca cacatgataa agcaacgttt gagaaaaact
    20161 acaaggcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
    20221 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaaca caaaatatct
    20281 ggggagatca tactgatcca tacggttatc tttctgaaat gggcgtaagt aaagaaaaat
    20341 tagcatatga tttagctcat ggatttaccg atgaaaatcc aacaacttct gaaaacaagc
    20401 ctgtcattga tccaacacga gctggtgcag ctaatcctac actgacagat ggaacgaatt
    20461 acgcccacat tgatcagttt ggagaaatcg aaaa
//
LOCUS       DAEGIQ010000047        19658 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000047 DAEGIQ010000000
VERSION     DAEGIQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.047"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            20..574
                     /locus_tag="KMT45_001517"
     CDS             20..574
                     /locus_tag="KMT45_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE7004985.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(747..1220)
                     /locus_tag="KMT45_001518"
     CDS             complement(747..1220)
                     /locus_tag="KMT45_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE7004986.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1232..1768
                     /locus_tag="KMT45_001519"
     CDS             1232..1768
                     /locus_tag="KMT45_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE7004987.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1808..2179
                     /locus_tag="KMT45_001520"
     CDS             1808..2179
                     /locus_tag="KMT45_001520"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE7004988.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2463..3734
                     /locus_tag="KMT45_001521"
     CDS             2463..3734
                     /locus_tag="KMT45_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBE7004989.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3979..5004
                     /locus_tag="KMT45_001522"
     CDS             3979..5004
                     /locus_tag="KMT45_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7004990.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5298..6407
                     /locus_tag="KMT45_001523"
     CDS             5298..6407
                     /locus_tag="KMT45_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBE7004991.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6490..7386
                     /locus_tag="KMT45_001524"
                     /pseudo
     CDS             6490..7386
                     /locus_tag="KMT45_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7402..8226
                     /locus_tag="KMT45_001525"
     CDS             7402..8226
                     /locus_tag="KMT45_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7004992.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8258..9568
                     /locus_tag="KMT45_001526"
     CDS             8258..9568
                     /locus_tag="KMT45_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HBE7004993.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9716..10975
                     /locus_tag="KMT45_001527"
     CDS             9716..10975
                     /locus_tag="KMT45_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBE7004994.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10985..11857
                     /locus_tag="KMT45_001528"
     CDS             10985..11857
                     /locus_tag="KMT45_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7004995.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNTNIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11869..12699
                     /locus_tag="KMT45_001529"
     CDS             11869..12699
                     /locus_tag="KMT45_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7004996.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12739..14922
                     /locus_tag="KMT45_001530"
     CDS             12739..14922
                     /locus_tag="KMT45_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBE7004997.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14983..16203
                     /locus_tag="KMT45_001531"
     CDS             14983..16203
                     /locus_tag="KMT45_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBE7004998.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16200..17660
                     /gene="gtfA"
                     /locus_tag="KMT45_001532"
     CDS             16200..17660
                     /gene="gtfA"
                     /locus_tag="KMT45_001532"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBE7004999.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17737..18843
                     /locus_tag="KMT45_001533"
                     /pseudo
     CDS             17737..18843
                     /locus_tag="KMT45_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19165..19374
                     /locus_tag="KMT45_001534"
     CDS             19165..19374
                     /locus_tag="KMT45_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBE7005000.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19367..19516
                     /locus_tag="KMT45_001535"
     CDS             19367..19516
                     /locus_tag="KMT45_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE7005001.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
BASE COUNT         6713 a         2965 c         3836 g         6144 t
ORIGIN      
        1 tttaagaagg gaaacaatag tgagctataa tgtacaaccg ttaaggacaa atgaagagat
       61 acaagatttt cagttttggc taagaagaac aagtaatccg gaaagagata gttttttatt
      121 tttgtttgga atcaataatg ggttacgtat gtcagatatt ataggattaa aagttggaga
      181 tatacgaggc aaaagtaagc ctattattgt tgaacataaa acaggtaagc gaaaaccgat
      241 ttttatagat aatttgagag aagaaatttt attatatact gaaggtaaag aagaaaatga
      301 ttggttattt ccgagtcggc aacaaggtcg acatataaca agggatcgtg tctatcagat
      361 atatgcagat atagcagaaa aattaggacg agatgatata ggaacacata cattaagaaa
      421 aacttttggg tatcactact acaagaaaac tagagacatt gctaccttaa tgtttatttt
      481 taatcatagc tcgcaagcaa ttacaaaaag gtacattggt ataactgaag atgaaatagg
      541 agcatcactt cgtggattta aactaggtgt ataatttaaa aatttttata aacagtaaag
      601 acaaagatta aaagctattt ttatctagaa cgattttatt tcataaagtt cgcggaataa
      661 aagctggggt tactggtact tggtataggt tcgtgttata taaactaagt aattaaataa
      721 catttttagg ttggaaaagg atgaaattaa aaaaagagaa tcaatacggt ctcaaaaacg
      781 caaacgctgc ggaataagaa aaatactgat aactcaacgt ttagcagact atctaatagg
      841 gatagtctgc ttttttcgtt tttgaccata cttttgacct tctgcaaact tccttgcttt
      901 tcctctgtag aactcatgaa atctgcaaaa cgaattgccg tttcctctgt ctgctttttc
      961 gcgatatggg tgtatatatc atccatagtg gttttaatat ctgtatgtcc aagtcgaaca
     1021 tacacttctt tgacagacgc accagattca aagagcagac tacaatgggt atgacgaaaa
     1081 ccatgaagtg tgatatgttt taagttattc tctttaatga tgattttcaa attgtgattt
     1141 acatagtcta aataaagggg cttattttct tctgatgaaa acaaaagttg ttcaccatct
     1201 tttataggtt tgattctcat ttttagatat tatgcccgca cgtattcatc acatttctat
     1261 tgtgaatcgc tttattcgac cgacatttga cttttatcat aatatactcg gtttaaaatt
     1321 actaatgaaa acaattaatc aagacgatca taccatgtat cacctgtttt tttcagataa
     1381 tcaccaccgt gtgggaactg aactgacttt ttttgaagta caagaaggga acaaccagtt
     1441 ttttggaacc aatacgattg aacgaacgat actaaaagtt ccttccgaag catcattgca
     1501 tttttgggaa atatattttg aaacacaagg cgtttgccat tatggaatcg aatcattttc
     1561 tggacgacct atcttacgtt ttgaagggcc agatgcaaca cgattagctt tagttccctt
     1621 acgcgaattt gaagatattg atgattattt cccgtatgtc acagaccaga tccctacaga
     1681 gcatgccatt ttaggaattg atgccattca actacgcgta caatattccg atgcgactga
     1741 acgagagtta ttgtcggtag gctggtagca caaagaatac gtcagtttct tttcgggtca
     1801 tcaacgagtg acaatcttag aaaatcaaga cactcagttc tatcaagaag ttcagattat
     1861 tcaagatcgt gagaatccag ttgccattga agggattggt agcgtccatc atgttgcgtt
     1921 tggcgtagaa aataaatctg atttacaacg catcgacaaa caattacaag agcgcaattt
     1981 tataaattca ggaataaaag atcgtgaatt ttttgtatca ctttattatc gtgacccaaa
     2041 tcaattattg attgaaattg ctacagggga aggaaaccta gatgcaaaag cgtatgaaaa
     2101 tcaaagtcca cgctttgaag aaattccttt atttttaccg cagtacttaa atttcattcg
     2161 tgaacaagtg gagcaataat tagctaatca aatccatgac gaagagtaag aggttgactc
     2221 ataagtcaaa aaataaagaa aaacgcatca aatccattct aaagaatgtt gatttgatgc
     2281 ggttcctatt tttataaact atcattttcg actaataaaa ctacttatga gtcagcctct
     2341 ctttttttct tgatttttat acctctttct aggtaaacta aacatctgcc aaaaataccg
     2401 tgtctcattg aaacattctc aactatttca aaataaaggg gagtaccaaa tgaacgagta
     2461 tattgaaatt atccacgcaa gagaaacaac ttaaaaatgt cagtctcaaa attcccaaaa
     2521 ataaaattag tatttttacc agcgtatctg gttcaggaaa atcatcgatt gtttttgata
     2581 ctattaccca agaagctggt cgtcaactga atgacaccta tgatagtttt acccgtttat
     2641 ttttacctaa atacagtcgt ccagaggttg atgagattga taatctttct acggcgattg
     2701 tggtcaatca aaagaagatt ggtggcagtg cgcgctcaac gttagggacg attactaaca
     2761 tcaatgcgtt atttcgtgtg ttgttttcat gttttgcaac acctagttta ggatatgcaa
     2821 atgcttattc atttaatgat cctagtggaa tgtgttctgt ctgtcaggga atcggtaaga
     2881 cgattgcatt tttaatagga ttgctttatt tttcggctat tgataatgct gattttgtat
     2941 tttctgatcc atttaccgca gaagttttgt ctggaattga ggaacaggca aagaatcatg
     3001 gttattttgt attggtgcat aatgttaatt cagcagaaga tgttaaaact attcagagaa
     3061 attggcgttt tgaggggttc attgttgttg gtgtacgtgt aaaagaattt ttggaattaa
     3121 ataacgtttt tgatggtcct gttgtttata ttgatacgca tctttcaaaa gagacattaa
     3181 gagaacttga acaacaaatg aatcgtttat ttgtaaatac aaatgattac caagctagta
     3241 cattggcagt ggaacatttg attcattatg gtcatgagaa aatagcgttt ctttcttttg
     3301 actacattgt aggtgaacca agtgttattc aagaacgaca tcaagcttat atcgatacat
     3361 taaagaaata tcatttaacc tttaaatcgc attggatata taacaactct gaatttgagc
     3421 gaatttatca agaattagct gaatttactg caattgttgt tactggagat tatttagcgg
     3481 ctaaattagt aaaattttta aaaaataaga ataaacgagt gatggaacaa ttatcgatta
     3541 ttggatttga tgatattact tttgcagaat taatggatcc tgcgttgaca acaatacgac
     3601 taaatccagg taacaagggg gcgattgctt ttcaccaact gattggactg gccaaacaag
     3661 aagaaataaa aaatcaagta ctactattag agggagaatt gatttctcga gactctgttc
     3721 acaaaatcaa ataaaaaagc tctgtgaaat aagtatagtt aacaataaaa tatccgaacg
     3781 atagagagaa tgaactgtga tctagcagtt cctcgtcgca aattcactag aattagcatt
     3841 atttactcta aattgttcgg atattttttc ttgtttttaa ttatgattac agtgcctatt
     3901 tttaacgaaa tttgcttatt ttcaaaatgt atgattatat gttagtaaac actttcaaat
     3961 atgaaaagag gagctattat ggtaggaatt agggatgttg caaaacgggc gggtgtgtcc
     4021 attgcaacag tatcgcgcgt gctaaatgag gatccgacac tttcagtaac cgatgaaacc
     4081 aaacgaaaga ttgcagaatc ggttaatttt tatcattatg tcaaaaaaag tcccgttgca
     4141 aaaaatttaa caaatattgc attaataacg acagtttctg aagtagatga gttggaagat
     4201 ccctattttc gaacgattcg cagagggatt caagtcgaag cagaacaaag caaagtcaat
     4261 ttgaagaaaa ttattcgttt gtcagaaaca ccgttggagc aagacgattt gctaaaatgt
     4321 caaggtgtgt tgattattgg gcaggtgcta tcttctgtga ttgaagaagt atcagcaatt
     4381 aattcgaatg tcgtagtgat tgatgaccct agtattgaag ctgaagtaga tgcagtttat
     4441 actgatttaa caaaagcaac gaatcagcat ttagatcgat tgtataaaaa aggacaccga
     4501 aacattgcgt ttatcggagg aggacgtgtt cgtttagata gccaaggaaa taaacacgtg
     4561 tcagatgatg attatcgaaa aatagcctat gagacgtgga tgaaacaaaa aggattagaa
     4621 aaatatagtc aaatttattt agaaggctgg accacacttg acggaatgca ggcagcagat
     4681 ttacttttga aaaaacatgc taaagataag ttgccgactg ctatcatggt aggaaatgat
     4741 cctattgctg tgggggttta tcgttcactt caaaaaaatg gtctacatat tccagaagat
     4801 atttcgattg tgagttttga taatattgaa gttgctgaat ttttaacacc tgcattaaat
     4861 acggtgaatg tgaatacaga agaaattgga aaactagcag ttcgcatggt taatgaacga
     4921 ataaaagaag ttagaaatgt ttcgatcaaa gtgattgttt ctaataatat tattgttaga
     4981 gaaagtgaaa aaaatatcga ttaaaaaagt aaacatccaa aaaagggtgt ttactttttt
     5041 gtttactaat atttttagta aaaaatctcg acaaaacatt aagtaaacag ttatggtgtt
     5101 gttaacaaaa agaaaacgca ttcaaaacag gaggaattaa gatgaaacat ttcaaaaaaa
     5161 tagctttaac agggatagct gttttaactg ggacattact cgcagcatgt ggtgggggag
     5221 atagtaattc taaagacggg caagtaactt taagctatgc gagttgagat actgaccaag
     5281 ctccaggact aagaaaaatg ttagatgaat ttgaaaaaca aaatcctgat atcaaagtag
     5341 aaatggagac aacgccttgg gatcaatatt gggttaaatt agaagctgca acaacgggag
     5401 gtaatatgcc agatgtagta accatgcact ctagcgaatc ctataaatat atgtcacaga
     5461 atgctttgat gggacttgat gatgttatca aagaaaatga tattgacatg ggaaacttta
     5521 ctgaaggaat tgctgatttc tatacgcttg aagacgaact atacgcaatc cctaaagatg
     5581 cttctgtagt ggggctatgg tacaacaaag aattatttga taatgcaggt gtcgattatc
     5641 cagatgaaac atggacatgg gaaacactca gacaagctgc aattgattta acagatgaat
     5701 caaaaggcat ctatggtttt gcatcacata ttggaacaga gactggctac tggccattta
     5761 tttatcaaaa tgggggagaa gtatttgccg atgacaatac aaaatcaggt ttgaataccc
     5821 cagaagcaca agaagcttta gctttttatc gagatattgc acttgttgat catgcttcac
     5881 caacaattac agaattacaa gatcctgata agttaagtcg ttttcaatca ggtagattag
     5941 caatgatgat tgaaggaaac tggtttgcac cgggattttt gaaaaatgac tatctagttg
     6001 aaaatgttgc ttctgccgtg ttacctaagg gaaaagttaa tgcaacaatt acaaatggac
     6061 ttggttgggc tgccagtgcg agtacaaaat acccagaaga agtgaaaaaa ctaatggcat
     6121 ttttagctag tgaggacgcc aataaaatcc aagcggaaac gggggcatca atcccagcac
     6181 taaaaggctt tgaagacgag tgggctgctt cttcagatac aattgatttg agtgcttttg
     6241 gaaaaatgtt ggagtatgga gtccctcgtc catacaataa agatggatta cgagctgaac
     6301 aagcagaagt tcaaattatt aaccgtattc tgtctggtga agtaactgtt gaagatggaa
     6361 ccgctcaaat tgaaaaagaa gtcaatgcga tattgagcgg aaattaaatt agttaattat
     6421 acaatataca ggcgaaagga ttctatttcg tctgtatatt aatggatgaa taccaaaaag
     6481 ggggatatta tgaaaaatcg aatttttgga aaatatcgta aagatcaagt gatatggggc
     6541 tatattttta tttttccaac ggcattagga ttaattttat taaatatatg gccagcaatt
     6601 caaacagcaa ttttaagttt tcaaaaaacg gtagggttcg gtgatactga atgggtcgga
     6661 ttaagaaatt accaaaaaat gtttgcagac ggtgaagttt ttcagtcttt attaaacaca
     6721 ttaatttatg cagtggtttc gatacctagt attgttgtat tatccttgtt ggtggcagtt
     6781 ttaatgaata gaaaagttaa aggattgtcg atttatcgga caatttattt tctaccagtc
     6841 gttgctgcac catccgcagt cgcaatgatt tggcgctgga tgttcaataa tgattatgga
     6901 ctgattaata acatgttggc taaaataggt ctcgatggac cagcatggtt aacggatcca
     6961 tcgattgcga tttattctat tatcattgta ggagtttgga gtgctattgg ttataacatg
     7021 gtgttattac tagccggctt ataagaaatt cctaaagatt attatgaagc agcggaaatt
     7081 gatggtgcta gtccaatcca gcagtttttc aacattacgt taccattagt tacaccaaca
     7141 ttattttttg tagttgtaac aacagttatc aatgctttcc aagtatttga tgttattttt
     7201 atgatgatta ctcctaatag taccgccatg tttaaatcac aatcattagc ctatttattc
     7261 tataaacatt catttatttt aaatgacaag ggatatggtt cggcaatcgt tatgttcctt
     7321 ttagtgatta tattaattat tactgcaatt caaatgaagt tgcagaaaaa gtgggtaaac
     7381 tattaaggag gctatcaaat tatgaaaaat aaattttttt caaatgcgcc aatccatatc
     7441 atgcttattt taggtggtat tgcaatggca ttacctttta tttggatgat tttaacttca
     7501 gggaaaacgt tggttgaatc tacccaaatt ccacctacaa ttttccctga gaaatttcag
     7561 ctaagtaact atcaacaagt acttgattta ttaccattca tggcttttta tatcaataca
     7621 tttttaatgg ttgttggacg agtgattggc tcggttttat tttcagcaat ggctgcatat
     7681 gcttgtgcgc gtctaagttt tccagggaaa aatatccttt tcagcttagt acttattcaa
     7741 atgatgattc ccggttccat ttttattact ccacagtatt tattagcaca aaaattgggt
     7801 attttaaata caattccagc attaatatta ccaggggtag ttagtgcgtt tggtacgttt
     7861 ttattaagac aatttttctt aggtttacct gatgaattgg aggaagcggc ctatttagaa
     7921 ggtgccaata tttggacgat ctttttcaaa attatgttgc cgcttgctaa atcaggattg
     7981 gttgcacttg gaatattcac agcgttgttt gcatttaaag atttaatgtg gccattgatt
     8041 gtcaatactt caattgataa gatgccttta gctccgggat tagcaaactt acaaggacaa
     8101 tatgccacga acttcccaca attaatggca ggttcgttat tagcaatttg gccaatagtt
     8161 gtgatattca tcgttttcca aagaaaattc attgaaggaa ttgctacaac aggtagtaaa
     8221 ttataaaact attaattagt gattggagag taagtacatg acaaaaatta catttattgg
     8281 tgcaggaagt actatttttg caaaaaacgt attaggcgat gcgatgttaa ctccaagtct
     8341 acaagatgca catattgcat tgtacgatat tgatgaaaaa cgactaaaag aatcaaattt
     8401 aatgcttaaa aatattaata aaaattcgaa tgaaaatcgt gctgatattg tttcttatta
     8461 tgatcgcaaa gaagcgttaa gagaggctga ttttgttatc aatgctattc aagtgggtgg
     8521 ttataaacct tcaacagtga ttgattttga tattcctaaa aaatatgggt tgcgacaaac
     8581 tattggtgac accatgggga ttggtgggat ttttagaacc ctacggacac taccagtaat
     8641 gttagacttt gctaaagaaa tggaagaagt ttgtccaaat gcttggttat tgaactatac
     8701 aaatccgatg gcgatgttaa ccatgggtgt tttaaaagcg acgagtatta agactgttgg
     8761 tttatgtcat agcgttcaag tatgcgtacc agaattgttt gagcatttag gaatagccga
     8821 tcaatatgac ttaaatgact ttcaatggaa aattgcaggc attaatcata tggcattctt
     8881 gttggaaatt acaaaaaatg gggaagattt ctatccaaca attcgtcagt tagctgcaga
     8941 aaaaccaaat ccacatccag attctgtacg ttttgaattg ttgaaacgtt ttggttacta
     9001 tattacagaa tcaagtgaac acaatgcaga ataccatcca tactttatta aatcaaaata
     9061 cccagaattg attgacgaat tagggattcc gttggatgaa tacttacgtc gttgtgaaca
     9121 acaaattaat gattgggaaa gcatgcgaga ggacattgta aataatgggg agttaacaca
     9181 tacgagaagc cgtgaatatg cttcatatat catggattct attacgacag gaactccgtc
     9241 agtgattgca ggaaatgtct taaataaagg cttaatcaca aacttaccga cagatgtttg
     9301 tgtggaagta ccttgtttag tcgataaaaa tggcgtgcaa ccaacctatg tcggtgaatt
     9361 gccaacacaa ttagctgctt taaatcggac gaatatcaat gttcaagaac tgacagtaga
     9421 agccgcttta acactagata aatcaaaaat ttatcaagca gcgtatttag atccacatct
     9481 atcatctgaa ttgtctttaa cagatatcaa aaatttagtg gatgatttaa tcgaagcaca
     9541 tggggactat ttacctgaat ataattaaaa agaagctgaa agagtaagga attttcaatt
     9601 ccttactctt ttcttagtta aatttagtaa aaatattgac aaaacactta gtaaacagtt
     9661 acattagtgt taacaaaaag aaaacgcatt caaattgtat aaggaggagc ataaaatgaa
     9721 gtggtctaaa aaaattattt tagcttgtat agcactagga gcaacgatct ctttgactgc
     9781 ttgtggaaaa aatcaggaag atgcaagcgg taaggtagaa atagagtttt ttaatcaaaa
     9841 aaaagaaatg actcaaacga ttcaggagat tgctaaagat tttgaagcaa aaaaccctga
     9901 tattcatgta aaagttgtgg atgtaccgaa tgctggtgaa gtaattaaaa cacgtatgct
     9961 tgcaggcgat gtaccagatg tcattaatct ttatcctcaa agtatagaat tgaaagaatg
    10021 ggcgaaagct ggttatttag aggacttaac cgaagaaccg tatttagaga atatcaaaaa
    10081 tggatatgca caacgttttg ctattgagga tcgtgtatat agcataccat taaccgctaa
    10141 tgtctatggt ttttactata acaaaactgc ttttgaggaa atggggattc aagcaccaga
    10201 aacttgggca gaatttgaaa aaatagtagc tgagattaaa gatcaaaaca aagtaccgtt
    10261 tgcgattgca ggagcagaag gctggacttt aaatggttat caccaactag ctatagcgac
    10321 tgctgctggt ggtgaagagg aagcgaacaa tgtttggcgt ttctcagaag ttaatggaat
    10381 taatgctgaa agcaaagaaa tgcaaaaaga ttttaatcga ttagatttgt taagagaacc
    10441 aaaagctttg caaaacaact ggcaaggtgc cggttataac gatacagtag ttacttttac
    10501 aaaaggtgat gcgttgatta tgccgaatgg ttcatgggca atgccgatga ttcattcaca
    10561 aaatccagat tttgaagtag gaacttttcc ttttccagca gatgaagcag gtcaaagtct
    10621 aacgattggc gcaggagacc tagccttatc agtttctgca acttctcaac ataaagaaga
    10681 agccaaacgt tttgtagaat atatgacaac acctgaagcg atgcagaaat attatgatgt
    10741 agatggtgca ccgtgtgctg ttgagggcgt tatcgagaat actcaggatt caccattaag
    10801 tggtttaact gaacttgctt ttactgatcg tcatttagtt tggttagcca aagattggaa
    10861 tagtgaaaat gatttttata ccttaacaac aaattattta cacaatggca atcaaaaagc
    10921 catgattaac gcattaaatg ctttctttaa tccaatgaaa gctgacggaa ggtaggaaaa
    10981 taaaatgaaa ataaaagaaa ttttaaatcg ttactgggca atcatttttg tagcaattcc
    11041 aattattttg cagatgattt ttttctactt tccaatggtt caaggtgctt tttacagttt
    11101 aactaattgg actggtctga cttataattt tgattttgtg ggtttgaaca attataagat
    11161 tcttttttct gatccgaagt ttatcaaatc gattggcttc actttaattg tgacgctttg
    11221 catgattgct ggacaaattg gttttggtat tttgattgcg cgggcgttga acacaaatat
    11281 taaagggaaa acattcttta gagcatggtt cttttttcca gcggtcttat cgggcgtggt
    11341 tgtttcttta gtatttaaac aaatttttaa ctatggttta ccaaaaattg gtagtatgtt
    11401 aggcattgaa tttttacaaa ccagtctttt aggaacaaca tggggagcga tttttgcagt
    11461 aatttttgtt ttactttggc aaggaattgc catgccagtc atcattttct tagcgggttt
    11521 tcaaagtatt ccacaagaaa ttattgaagc tgctagaatg gatggggcga atgataagca
    11581 aattttttgg aatattgaaa tcacctactt attaccaagt atttcaatgg tatttattat
    11641 ggcattaaaa ggtggcttaa cagcatttga tcaaattttt gctttaactg gtggtggacc
    11701 aagcgatgcc acaacatctt tggggttact tgtttataat tatgctttta aaaacaatca
    11761 gtttggttat gcgaatgcta ttgctttagt cttatttatc ttaattggga ttgtttcact
    11821 ggcacaatta aaattatcga aaaaatttga aatctaggag gtttcccaat gaaaacatca
    11881 aaagaaaaaa atatttctaa atacttgctt ttattattag ggggcttcat gattttagtt
    11941 cccttagttt caacgctatt cggttcattt aaaacaacac ccgatattat gaataatttc
    12001 tttcgttttc ctaatccagc gactttggaa aatttccgaa gattgttagc ggatggaatt
    12061 ggtcattact tttggaattc ggcaatcatc acaatcattg cagtagttgc tgtgacagca
    12121 attattccta tggctgctta ttcactagca agaaaaatga ctcgtgggaa agcatacgga
    12181 ttgatgtatt tatttttaat tttaggaatt tttgttcctt tccaagtaat tatgattccg
    12241 attaccgtta tgatgagtaa aattggttta gcaaatatgt ggggattaat cttactctat
    12301 ctcacttatg ccgtaccaca gacactattt ttatatgttg gttttattaa actaaatgtg
    12361 cctatcagtc tggatgaggc tgctgaaatt gatggggcta gtcactttac aacttatttc
    12421 aaaattgttt tcccaatgtt aaaaccaatg catgcgacga ctattattct taatgctcta
    12481 tggttttgga atgatttcat gctaccatta ttaattttaa atcgtgattc taatatgtgg
    12541 acactaccat tgtttcaata taattatacg ggacaatatt ttaacgacta tggtccaagt
    12601 tttgcttcct atacagtagg aattgtcacg attacactag tttatcttgc tttccaaaga
    12661 agtattatag ctggaatgag caatggagca gtgaaataac caataaatta ttctcattat
    12721 aaaggagttc ataaacttat gattatattt gacaatcaca cacaaacatt tcatttacaa
    12781 aataaaaaaa ttagctatct attaggaatc gaagaaaata attatttaac gcatctttat
    12841 tttggaaaaa agattacgca ctattctggt gggtatcgct acccccgaac agatcgttcc
    12901 ttctcaccca acaatgcaaa tgcgcaagat cgtttattat ctttagacac attaccatta
    12961 gaatttccag gatatggaca tggtgatttt agagaaccgg cgcataatat taaattagga
    13021 aacggtagtc gtgtaaatga ttttcgttat gatagctacg aaattcaaaa aggaaagccc
    13081 agcttagcgg gattgccagc cttatatacg gaagatgatg atgaagctga aacgctgatt
    13141 attcaattga aagacgcgat tagtggattg actttacagt tgttctacac aatttatcaa
    13201 aatcacagtg taattattcg ttctacgcaa ttagtaaata atagtgatga aacagtggag
    13261 attaaccgct tagctagtca atcaattgat tttccaaatc gtccactcga gttaattcat
    13321 ttaaatggta cctgggcaag agaacgtcaa atgactagag aaaaaattca tacagggata
    13381 aaagtattag atagtaaacg cggatcaagc agccatcaac aaaatccgtt tgttgcttta
    13441 gtagatgcca aaacagatga atttcaagga gaggcatatg gatttaattt agtttatagc
    13501 ggtaatcacg aaacggtgat tcaaaaagat ccatttgacc aaacacgggt cattatggga
    13561 attaattcat ttaattttgg ttggcaacta aaaactggcg actcatttca aacgccagaa
    13621 gtcgtgatgg tttatagtaa tgaaggtctg aatacgttat cacagacgta tcatgaattg
    13681 tataatcacc gtttaattcg tggaaactat cgtttaaaag aacgtcccac gttaatcaac
    13741 aactgggaag caacgtattt tgattttaac gaagaaaaaa tcatgaatat tgtcgatgat
    13801 gcgaagaact tgggcattga aatgtttgtt ttagatgatg gttggtttgg cggtcgtgaa
    13861 aatgacttca cttcattagg tgattgggtt gaaacagaag gcaaacttgc ttgtggtttg
    13921 gaaaatttag ccaagcaagt gcatgataac gggatgaaat ttgggatttg gtttgaaccg
    13981 gaaatgattt ctgaagatag tgaactattt cgagcacatc cagattgggc attgggtatt
    14041 ccaggtcgtg accgctcaat tagtcgaaat caatatgttc ttgatttttc aagacaagat
    14101 gttcgtgata atatctatca acaaatgacg gcgatttttg atcgtgtacc gattgattat
    14161 gtgaagtggg atatgaatcg aaatatgaca gaagtctatt ctgtcttgtt ggactcaacg
    14221 atgcaaggtg aagtggctca tcgttatatg ttagggttat atgaattttt agaaaagtta
    14281 acaacgaatt atccaactat tttatttgaa agttgttcgg gtggaggcgg tcgttttgat
    14341 gcaggtttct tgtattacat gccacaaact tggacgagcg acaataccga tgccgtggct
    14401 cgtctgaaaa ttcaatatgg aaccagctta gtgtatccaa tttcaagtat gggtgcgcat
    14461 gtttcagcag tcccaaatca tcaaacagga agagaaacga gtttaactat tcgtggcaat
    14521 gctgcgatgg ctggtgtttt tggttatgag ctagatctaa cgcaattaac ttctgaagaa
    14581 aaagcagaaa ttaaagaaca agttgccttt tataaaaagc atcgccaact tttgcaatat
    14641 ggaacttttt atcgcttgga aagtccgttt gaaggcaatg atactgcatg gatgtttgtc
    14701 tcaaaagacc aaacagaagc catggtcttt tatttccgag tattagcaga agctgcgcat
    14761 ccattagtca cattgaaatt agcaggatta gcagaagaag caacttatca aatggaagat
    14821 gtaacatttg ctggcgatga attgatgaat ctcggttttt atattgactc agaactacat
    14881 ggtgattatg cgacacaacg cttttatttg aaacagatat agtagcttga atttacaata
    14941 taagtgcaac accaaaagag actcattcca aacgaagaaa atatgtttta tttacacgca
    15001 tttactaaaa agcagcctga tttgaattgg gaaaatgaag aagtacgtga agaaatctat
    15061 gcaatgatta actattggtt agataaagga ctaggcggat ttagaatcga tgcgatatta
    15121 aacatcaaaa aaaaactaga gtatggcatc tttgaatctg atggagaaga tgggttagcc
    15181 ttcattgggc cctggattct taatcagtcg ggcattgaag tgtggttaaa agaaatgaga
    15241 gagcggacat ttaaaccaca taatagtatg acggtagcag aagccgatgt tcctaatgaa
    15301 cgtttagatg aatatattgg tgaagatggc tattattcga tggtttttga ttttagttat
    15361 acggatattg atgtacctga aactggtgaa tggtttaaag atagccaatg gacatggacg
    15421 gatatgcgta ccaatatttt tacgaatcaa ttagttacgc aagataaagg ttggggcgcc
    15481 ttatatttag aaaaccacga tcaaccacgt tcaattaata aatatattcc agaagaatgg
    15541 atcaatgatt atagtaaaaa aatgttggcg acgcttttta tgttactaag aggtacccct
    15601 tttatttatc aaggacaaga actaggaatg accaacatag aaatggcatc attagaagat
    15661 tttgatgatg tagctacgca ttctcagtat aaacgggcat tagaatatgg attggcacca
    15721 gagcaagcat tgaaagctgt caataaacga agtcgggata attctcgcac acctatgcaa
    15781 tggaatacgc aaaaaaatgc cagattctca acttctgata atgtatggtt aaaagtaaat
    15841 cctaattatc cagaactcaa tgcagaagct cagttaacca atcaacagtc ggtattcaat
    15901 tattatcatc aattaattaa attaagaaaa aatagtgtat acaaagacgt gctaatctac
    15961 ggacaatttc taccagtgaa agataaaaat gaacatattt tgctttatga acgccaatta
    16021 gaagataaaa tcgtattagt tatcttaaac gttacagcaa cagaacaaga atattatgta
    16081 gatgaacagt accaacaagt attaatcaat aattatgaag atgttttatt agatcaaagt
    16141 cgaaaattgc gtttacgacc gtttgaaagt attgtattag ctaattatgg aggacaatta
    16201 tgaaaattaa aaacgaagca atgctcatca cctatgcaga tagtttagga aaaaacatgg
    16261 gcgaattaaa acaagtctta gacaaacatt tacaaggtgt tattggtggt gtacatctgt
    16321 tgcctttttt tccatcgaca ggtgatcgag gctttgcgcc gagtgattat acgacagttg
    16381 atccagctct tggaacatgg gaagatgtgg aagcattagg tgaagattat tatttgatgt
    16441 ttgattttat gattaatcac atttctcgtg aatcaaaatt tttccaagat ttcaaaaaaa
    16501 accatgagca gtcaccatac aaagaaatgt ttatccgtat tcatgaattt ttcccagaaa
    16561 atcgaccaac acaagaagat attgatttaa tctacaagcg taaagacaaa gcgccgtttc
    16621 aaacggtaga atttgcggat ggtacgacag aagaggtttg gaataccttt ggggaagaac
    16681 aaattgattt agatgtgacc aaagaagttg tgaaagaatt tattcgagaa accatcaaag
    16741 atatggcgaa ccatggctgt tctttaattc gattagatgc gtttgcttat gcgattaaaa
    16801 aattagatac caatgatttc ttcgtggaac cagatatttg ggaactttta gacgaagtgc
    16861 gtcaagaagc agcgaaatat gacgtcgaat tattaccaga aatccatgag cattattcca
    16921 ttcaaatgaa aattgccaat catgattatt atatctatga cttcgccttg ccaatggtca
    16981 ctctgtattc actttatagt ggaaaatcag aacgtttagc caattggttg aaaatgagtc
    17041 cgatgaagca gtttacaaca ttggatacgc atgatggaat tggtgttgtg gacgctcgtg
    17101 atttattaac agatgaagaa ttggattaca cctctgagga attgtataaa gtcggggcga
    17161 atgtgaagaa ggtctattca agtgccaatt acaataattt agatatttat caaattaaca
    17221 gcacctatta tagtgcatta ggcgataatg accgtagcta tctgttagca cgtgcgattc
    17281 aagtatttgc gccagggatt ccacaaattt attacgttgg gttattagca ggaaaaaatg
    17341 atattgaact gttggaagca acgaaagaag ggcgtaatat taatcgtcat tactatgatt
    17401 tagccgaaat tgaagaagaa gtgcaacggc cagtgattca acaattattt gcacttttaa
    17461 aatttagaaa tgaatctgcg gcgtttgatt tggatggaac gattgaagta gcaacgccat
    17521 cagaatcaga aatagtgatt acacgtaaaa ataaaaacgg tagtgatagt gcaacattaa
    17581 cggctaactt ggatacgaaa gaatttatca ttgagtctaa agatataaaa attttcacta
    17641 gtgttgcatt aaaattttaa cctattccat agaacaaaaa atcctttata ctagcacttg
    17701 gatgacttat ccaagaccaa taaaaaggac tcatacatgg ataagtataa aacaaataca
    17761 gcatttaata aatggttttc tgccataaaa ttaggaaaac tttctgtcaa ctcccaaaaa
    17821 tgtatttttg attacaattc gtatcgtaaa aaactgtctt ttgaagctgt gctcaagctc
    17881 tttctctacg cagttaatga agaaaaagaa agtttgcgtg atttgagtac agctcttatc
    17941 gaccgatcgc tccaactaga aaccgatgtg tcatcgatta gtcataccca attgtctcga
    18001 gcatttaatg cacttgaacc taaagtgtta gaagaaattt ttcaacaact gttagaaaaa
    18061 gttcaacatc aaatcaaacc aaccaagcga aatcatctgt atctgattga ttccagtacg
    18121 ttttcgctta gtttaaaacg tcataagtgg gcgactttcc gtcaaacaaa atcaggtgtc
    18181 aagttacact taaactattg ttatatggac gatactacga tgtatccaac tgattttacc
    18241 ttaacaaatg cccatgaaca tgatctcaat caactaccag tattggtgaa tcaaccagag
    18301 gcaacttacg tgtttgatcg tgggtattag gactttgaaa agatggatca gatgcattgg
    18361 gatggctatt tcttcgtcac tcggattaaa aaaaacacca aagttcacgt gatagatact
    18421 ctggaaacat cgccagaaac agaaattcta cgagacgagc tggttcgttt aggatctaaa
    18481 acctatctaa ccgccaattt ccgactagtc actgttcaag ataagaacgg acgtgtgttt
    18541 caatttatca ccaatcgaat ggacgtctct tcaaaagaaa tctctgatat gtatcacgct
    18601 cgttggcaaa tcgaattgtt tttcaagcac atcaaacaac acatgaccat taaaaccttc
    18661 ttttctcaaa gtgaaaaagg cgtgcagaat caattgattt taacgatgat ttccgctctc
    18721 ctcacgtttc tcatcaaatt ggagacgaaa acggaaaaat cagtgttcca aatcaaacga
    18781 ttctttcgtt atttactgtt tcagcccttt gaatgttgtc tagaaaaatt aattcccaca
    18841 tgagaaaaaa gaaatttggc tcgttacttt tggttttcag ttgaataaca gataaaaaat
    18901 tttctggaaa aagtcatcct ctgcgtcagc acagtttttt ttgacttatt ttcagaggaa
    18961 atagttttca gaatattcta aaatgtttta tgcaacacta gtgatatttt ttagaattag
    19021 atttctaatg tgcatttttt gagtattaag gagcaagtat acaaagattt ataaagctga
    19081 ttaaatttga ataaaaatat gcaggaacgc tactattttt agtagtgttc ttttttatat
    19141 ttgaataaaa aatgtggtgg ttaaatggtg gttataacaa aaaatgaatt aatgaaatta
    19201 ggttatggga agtatcagtc tgagagaatc atacgacagg caaaagcaat tatggtttca
    19261 aaaagctata cctattacga aaatagaaga ttaggactag tacctgttga atctgtagaa
    19321 gaagttttag gtatgaagat agaggtagag gaggattttc agaatcatgg ttaattttaa
    19381 aaaagataaa aatgggaaat atcattttac tgctagtttg ggattcgaca aacgaacagg
    19441 gaaaagagta caaaaaaacg tagaggggtt aaaacagtta aagaagaaaa aaagcgtata
    19501 ccgaacttgt ttctaattat ggaaaagaag cagaaacttc tcaaagtact atgttgtttg
    19561 aggagttttt ttataaatta ttcctacctt attatcaagg gaaagtagaa gaaagaacat
    19621 ataaaaatcg gttatctaca atcaaaaaaa cttttctt
//
LOCUS       DAEGIQ010000048        18937 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000048 DAEGIQ010000000
VERSION     DAEGIQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.048"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(264..1229)
                     /locus_tag="KMT45_001536"
     CDS             complement(264..1229)
                     /locus_tag="KMT45_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBE7005002.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1463..2308
                     /locus_tag="KMT45_001537"
     CDS             1463..2308
                     /locus_tag="KMT45_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE7005003.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2499..3686
                     /locus_tag="KMT45_001538"
     CDS             2499..3686
                     /locus_tag="KMT45_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBE7005004.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3692..4048
                     /locus_tag="KMT45_001539"
     CDS             3692..4048
                     /locus_tag="KMT45_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBE7005005.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            4050..4388
                     /locus_tag="KMT45_001540"
     CDS             4050..4388
                     /locus_tag="KMT45_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBE7005006.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4464..4720
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4783..5817
                     /locus_tag="KMT45_001541"
     CDS             4783..5817
                     /locus_tag="KMT45_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7005007.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5810..6496
                     /locus_tag="KMT45_001542"
     CDS             5810..6496
                     /locus_tag="KMT45_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7005008.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6520..7368
                     /locus_tag="KMT45_001543"
     CDS             6520..7368
                     /locus_tag="KMT45_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBE7005009.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7565..8338
                     /gene="sufC"
                     /locus_tag="KMT45_001544"
     CDS             7565..8338
                     /gene="sufC"
                     /locus_tag="KMT45_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBE7005010.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8351..9637
                     /gene="sufD"
                     /locus_tag="KMT45_001545"
     CDS             8351..9637
                     /gene="sufD"
                     /locus_tag="KMT45_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBE7005011.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9634..10869
                     /locus_tag="KMT45_001546"
     CDS             9634..10869
                     /locus_tag="KMT45_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBE7005012.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHMTIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VIAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFKHGTI"
     gene            10856..11326
                     /locus_tag="KMT45_001547"
     CDS             10856..11326
                     /locus_tag="KMT45_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBE7005013.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11331..12722
                     /gene="sufB"
                     /locus_tag="KMT45_001548"
     CDS             11331..12722
                     /gene="sufB"
                     /locus_tag="KMT45_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBE7005014.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12817..13959)
                     /locus_tag="KMT45_001549"
     CDS             complement(12817..13959)
                     /locus_tag="KMT45_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE7005015.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13998..14732)
                     /locus_tag="KMT45_001550"
     CDS             complement(13998..14732)
                     /locus_tag="KMT45_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005016.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIIEEFDNNILLLTSSIEEQLEEMIDEGIHSQVEDMNYPEEAVNEIIDN
                     INKLKQTISSIGKKYTKKKMKGGDTI"
     gene            14967..15242
                     /locus_tag="KMT45_001551"
     CDS             14967..15242
                     /locus_tag="KMT45_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005017.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMEQYKG"
     gene            15340..17337
                     /locus_tag="KMT45_001552"
     CDS             15340..17337
                     /locus_tag="KMT45_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005018.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(17413..17748)
                     /locus_tag="KMT45_001553"
     CDS             complement(17413..17748)
                     /locus_tag="KMT45_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005019.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            17875..18090
                     /locus_tag="KMT45_001554"
     CDS             17875..18090
                     /locus_tag="KMT45_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005020.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            18127..18489
                     /locus_tag="KMT45_001555"
     CDS             18127..18489
                     /locus_tag="KMT45_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBE7005021.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADEVKKSTEQTFS
                     EMKEQIVHGQETGKLYLIIGWVSMVVSLAFIPVVFGAVAVIMGYLYRETDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            18535..18861
                     /locus_tag="KMT45_001556"
     CDS             18535..18861
                     /locus_tag="KMT45_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005022.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
BASE COUNT         6252 a         3279 c         3956 g         5450 t
ORIGIN      
        1 acctcttggt aaacggtgtt ccagaagtag cttcttcgga aataagccga aatttcacaa
       61 aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt
      121 acgacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta
      181 caaaaacatg aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt
      241 ttcacaacct attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg
      301 ctatgttctt taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt
      361 gcaaacagac attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg
      421 ttgaagtaat gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag
      481 gctgcatagc ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg
      541 aaatacaata cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca
      601 aagtttttaa gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc
      661 ggacgtgtcg gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat
      721 aagaacgcat agcagatcaa tgaaatccaa aagaaaattt ttccaataag cgggaagaag
      781 ttcccggcag ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa
      841 attgcttcaa ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg
      901 atcacttgga agataacagc gcacagccaa atgaatttga caattggtgc aatactttca
      961 tatgaaacaa aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag
     1021 gcgatgattg gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca
     1081 aaaatgaata tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg
     1141 tgatagtctt taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg
     1201 atcggaataa ttttcaaaaa gttcttcata tataatattc tcctttttat ttaatagatt
     1261 ttagtaccaa gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag
     1321 cagacagctg attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca
     1381 tttctattat agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat
     1441 aaaaagttat ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga
     1501 aacaagaaat ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa
     1561 tcaaatcaaa cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa
     1621 agagatgctt ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga
     1681 agaatgggaa gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg
     1741 gatcgttgct tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat
     1801 caaaaggttt cctaaagtgc attttgagat cgaaatcgcg aattctcatg aatcattaga
     1861 acaagtagcg aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg
     1921 cgtacaaaga tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa
     1981 attgtggctt gtaagagaag caaactctgg tgtttttcat tatacgcagc gttattttga
     2041 agagatgaat atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt
     2101 gctgaaggaa ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc
     2161 aatgactccg ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc
     2221 tgatcagctg ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc
     2281 tcacagcgag gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat
     2341 acactaaaga taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga
     2401 aagttaattt ttcccaaatg tctatggaaa gactttccat tatctaactg gaccgctata
     2461 ataatcagca gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt
     2521 cgaacgataa tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg
     2581 gtctgctctc attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag
     2641 gcgtagggat gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc
     2701 atttgaattc caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg
     2761 taatgggact cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa
     2821 actggtttcg attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca
     2881 tcttgatgat ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga
     2941 aatcggactt ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga
     3001 tcatggccca agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt
     3061 tcttgatgtc tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg
     3121 ttggagcagg aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttacaaag
     3181 ttggcttcaa atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata
     3241 cgtcaggaat gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt
     3301 ttggtaaagg attcaacgta agcaatattt atgtgcctgt tcgtgagtcc gatatgattt
     3361 ttaccgttat cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt
     3421 ttattttaat ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct
     3481 atatcgcaag cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa
     3541 atatcggatt acttccttta acagggatcc cattgccttt cattagtcag ggaggatctt
     3601 cgattttagg gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag
     3661 cacctgcaag atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg
     3721 ttcaacatgc aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaactgt
     3781 tgatatgatc aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca
     3841 agtaccaatc cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa
     3901 agatcaagta ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat
     3961 gctgatcaag cggccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga
     4021 aaaaatttat gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca
     4081 acctttggat cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag
     4141 aagaacttgg agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag
     4201 atgaacctct gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa
     4261 cgggtacagt ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt
     4321 cagatgaaat gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg
     4381 aattataaaa tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa
     4441 tcataagcaa tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg
     4501 tttgaagaga gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta
     4561 gagcagagta agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt
     4621 atacggaaag aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa
     4681 tcaaggtggt aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag
     4741 gcttttttgt ttgaaaaatc ataagagaaa gatgtgaatg gcatgtcatt aatccaacta
     4801 acgaatgtca aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt
     4861 tcactaaacg ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa
     4921 agtacattag tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta
     4981 aacgatcaag atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc
     5041 ggtatgatct tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag
     5101 ctgccgctgg aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg
     5161 ttgcgtttag tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga
     5221 cagaaacaac gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt
     5281 gatgaggcta ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta
     5341 gcgatcaata agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc
     5401 cgcaaaatct gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac
     5461 gtattgactg tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac
     5521 atcgaaccag aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc
     5581 ggccgtttgg caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa
     5641 gcgattcgac gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa
     5701 gaaggttcct ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa
     5761 gcagaagtgt tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag
     5821 cttttcagaa acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat
     5881 ccttgataca ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt
     5941 tttgggattg atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta
     6001 tggaatcgta tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt
     6061 tttgatcatt ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat
     6121 tccagctctt gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg
     6181 tgaagtcagc cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat
     6241 catctggaag gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac
     6301 aacgatctcg atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg
     6361 aggtcttgct tggcaggaag gctaccagcg tggtaatctg acagtgacat tcgttgcgac
     6421 tttaattatt ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa
     6481 aacagacaaa agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga
     6541 tttgcagcag cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc
     6601 aaagattcag caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg
     6661 cacgcagaga ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa
     6721 atcgtcaaat tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat
     6781 gccaattact tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac
     6841 tttgtcaatg ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa
     6901 gatgtttccg aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg
     6961 ggacgcatca tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat
     7021 ctggaaacag caacattcga tgatattgaa gaaaacccta agaacttgaa gttcaaccat
     7081 acgatcgacc catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca
     7141 atcaacgcaa acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt
     7201 gaaaaggaca actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt
     7261 gacaaaatca aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca
     7321 gacaaatggg gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa
     7381 aaaagatgag aaataagtct gtaatggctt atttctcatc ttttttatac ctattctaat
     7441 ttagaaatca aaattgttta ataataaata aaagatattt gattttagta aggaaggtca
     7501 ttgcgcttca ttctcaatta gtctaaaatg agtgagaacg aaagagaatt tgggggtata
     7561 atgcatgtct gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa aagaaatcct
     7621 taaaggcgta aacctaacaa tgaaaaccgg agaaatccat gccatcatgg gacctaacgg
     7681 cacaggaaaa tctaccttat ctgccgctat catgggcaat ccaaactatg aagtaacaga
     7741 aggcgaaatt ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg aacgtgcgcg
     7801 acttggctta tttttagcta tgcaatatcc aagtgagata ccaggtatca cgaatgcgga
     7861 attcatgcgt gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt ctgttatggc
     7921 atttttgaaa aagttagatg aaaaaatggc tttactaaat atgccagaag aaatggctga
     7981 acgttatctg aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga tcctgcaatt
     8041 gttgatgtta gaaccaacat ttgcaatttt agacgaaatc gattcaggtc tggatatcga
     8101 tgctttgaaa gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt tcggtgcatt
     8161 gatcatcacc cactatcaac gattattgaa ctatatcaca cctgatgtcg ttcacatcat
     8221 gatggaaggc cgagtagtca tgacagggaa tgctgaccta gctaaacgtc tggaagcaga
     8281 aggctacgct ggtatcagta aagaattagg aatcgactat aaagaagaag aagcgtaagg
     8341 gggagcaaga atgaaagaaa aaaactggct cgattatctt gacgcggtaa atgatttttc
     8401 tttaagtaaa ggcgaacctg attggatgag aaccttccgt caagacgcac tagcgaaagc
     8461 agatgaactg ccattgccgc atatcgatcg ggtgaaattc catcgctggt cgttatttga
     8521 tgtgaaagaa acacaaacca tcagtgaaac aggcacgatt ccggcatttg atgcaatgaa
     8581 agacaatcct gtgcttgtcc aacaaggttc atggacgatt tttgaacaac tgcctgtgga
     8641 attagcagaa aaaggagtca tttttaccga cttgttcact gcaatgatcg aatatccaga
     8701 actagtccaa gaatattata tgaaaaaagc agtaaacatg aacgaagacc aattgactgc
     8761 actccatgta gcttttatga acagcggtat tttcctgtat gtgcctaaaa atgtcgtgat
     8821 cgatgaacca ctcgagtcct tattcattca agatggcgca tccgatgagc acttcttcaa
     8881 gcatgtgttg atcgtggcag atgaacatag tgaattttct tatctggaac gtttccagac
     8941 aaccaaagaa caagtagcaa aatcatcagg taatatcatc gtggaagtca tcgctaaagc
     9001 tggttcaaaa atcaaatatt cagccgtaga tcaattagga gaaaatatta cttcttatat
     9061 gaatcgccgt gggcacattt tacgagatgc ttctgtcgac tgggcaattg gtgtcatgaa
     9121 tgacggtcat gtgatcgctg actttgactc agatttagca ggagaaggtg cacacgcaga
     9181 agtcaaaatc gtggcaatca gctcgggcag acaaatccaa ggaatcgata cgcgagtaac
     9241 caataaagcc cctcatacga tcgggcatat tttgcaacac ggagtgatcc gagaaaaagg
     9301 aacattgacc tttaatggta tcggccacat cttgaaaggg gcaaaaggtg cagatgccca
     9361 acaagaaagt cgtgtcttga tgctttctga taaagcacgt ggtgatgcga atcctattct
     9421 tttgatcgat gaaaacgaag taactgctgg acatgctgcc agtgtaggac gagttgatcc
     9481 agaagaaatg tactatctga tgagtcgagg attgcataaa gaagaagcag agcgcttagt
     9541 gattcgagga ttcctcggtt ctgtcttaac agcaatccct gtagaacaag ttcggaaaga
     9601 attagtcgaa gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa gctacgcaat
     9661 gattttccta tcttatttca agacgtcaat gatgaacctt tagtttatct ggataatgca
     9721 gcgacgaccc aaaagccaaa acaagtactc caggcattag cgcactatta cgaacacgat
     9781 aacgccaatg ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca tgattatgaa
     9841 caagcaagag aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga agtgctgttt
     9901 actcgtggta cgacgacaag cttgaattgg gtagccagaa gcttcggcga ccgctatatc
     9961 aaagcaggag acgagatagt gctctcttac atggaacatc attctaatgt tatcccgtgg
    10021 cagcagttgg caaaacgaac aggtgctgtc ttgaaatata tcgaactaac agaagaaggc
    10081 ttcctggata tggaggatgc agcgaaaaag attacggaca aaaccaaaat cgtctccatt
    10141 gctcatgtat ccaatgtctt aggtgtcatc aatcctgttc aagaattagc tgaacttgtc
    10201 catgaaaacg gcggggtcat cgtagtagat ggtgctcaat ctgccccgca catgaccatt
    10261 gatgttcaag cactagactg tgatttcttt gctttcagcg gacacaaaat gtgcggacca
    10321 acaggtatcg gtgttttata tggtaaacga aaatggttag aagaaatgga acctgttgaa
    10381 tttggtggcg aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa agaactacct
    10441 tggaaattcg aagcagggac accaaatatc gctggcgcta tcgcattagg tcatgcaatc
    10501 gattatttgc aagaactcgg catggacaat atccatcgat atgaagaaga actagccgct
    10561 tatgtgttgc caaaattaca agcaatcgat ggattgactg tttatggtcc gcaagacccg
    10621 aattttcgga caggagtcat tgcgttcaat ttggatgggt tacatcccca tgatgtcgcg
    10681 acagctttag acatggaagg ggtagccgtt agagcaggtc atcattgtgc gcaaccgcta
    10741 ttgaagtacc ttgaagtatc agcgactgct cgcgccagct tttatttcta caacacaacg
    10801 caagacgccg accgtttagt tgaagcaatc atggcaacaa aggagttttt caaacatggg
    10861 actatctaaa ttagacaatt tataccgtca agtgatcttg gatcattctt cccatccaca
    10921 tcatcgtgga agcttgactg actcgacaag caagatcgaa ttgaacaacc ccacatgtgg
    10981 agatgtgatc gaattgcaag ttgatgtaaa agatggaatc atcaaaaata tcgcctttga
    11041 cggcagcggc tgttccatca gtacagcaag tgcctcgatg atgacagatg ccgtcattgg
    11101 aaaatcaata gaagaagcag aacatttaac tgtagacttt ctacagcttg tccaaggaaa
    11161 agaagtcaaa gatcccgacc aattaggcga tgctgctatg cttggtggtg tagctaaatt
    11221 tcccgctcgg atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag caatggaaaa
    11281 cgatggtacc gcagctttcc atcattacca caaggaagag gagtgagaac atgggcgtac
    11341 cagaattaga agaatataaa ttcgggtttc acgatgatgt cgaaccggtt ttcagtacag
    11401 gagaaggctt gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc gaaccagaat
    11461 ggatgcttga attccgtctg aaatctctag aaacattcaa taaaatgcca atgcaaacat
    11521 gggggcccga cctttcagac atcgatttcg atgccatcaa atattaccaa aaaccaagtg
    11581 acaaacccgc tcgtgactgg gaagacgtac cagaaaaaat caaagataca tttgaaaaga
    11641 ttggtatacc agaagctgaa cgagcttatc tagccggtgc ttccgcacaa tatgaatcag
    11701 aagtcgtgta ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc tttactgaca
    11761 ctgactctgc tttgaaagaa taccctgaat tattcaaaga gtacttttca aaacttgtac
    11821 cgccaaccga caacaaatta gcagcgctga attcagccgt atggtcaggt ggcaccttta
    11881 tttacgtgcc aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc cggatcaatg
    11941 cggaaaacac ggggcaattc gaacgtacgc tgatcattgt tgacgaagga gcaagcatcc
    12001 actatgtcga aggttgtacg gcacctactt attcaagtaa tagtctgcat gctgccatcg
    12061 tagaaatctt tacaagaaaa gatgcgtatt gccgttacac cacgatccaa aactggtcag
    12121 acaatgtgta caacttagta accaaacgag caaaagcata cgaaggcgca accgttgaat
    12181 ggatcgacgg aaacttaggt gcaaaaacaa cgatgaaata cccaagtgtt tacttagatg
    12241 gaaaaggtgc acgaggcacc atgctctcca tcgcctttgc aggagcaaac caaatccaag
    12301 atacgggtgc aaaaatgatc cataatgcac caaatacatc tagctctatc gtctctaaat
    12361 caatttcaaa agacggcgga gaagtaaact atcgaggaca agtaaccttt gccaaaaaca
    12421 gtgcgggttc gatctctcat atcgaatgtg acacgatcat catggacgaa tggtcgaaat
    12481 ccgatacgat cccattcaat gagatccaca acagccaagt ctctttagaa cacgaagcca
    12541 aagtctcaaa aatctcagaa gaacaactct actatctgat gagccgaggc ctgaccgaat
    12601 ctgaagcaac tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa gaacttccaa
    12661 tggaatatgc agtcgaactc aaccgattga tcagttatga gatggaaggc agtgtaggat
    12721 aaacttgtta taggagctag aatccttggt atataagggt tctagctttt ttattttgtc
    12781 tatatactag aaatgaccag cttttgacca gctcttttac tgatgagcca tcatatcagc
    12841 aaagtcttga cctgttttct taggtgcatt atttgtaata tgtgcataga tgtctaatgt
    12901 cactttacta ttttcatgcc ctaatcgttg ttgaactacc ttgacagatt ctcctgcttc
    12961 taataataaa ctagcatgtg tatgtctaaa cccatgaggt gtaatacgag gcaagttata
    13021 ttttttcaaa atatatctta accaatcatt aacagtgttt ggaatgtata gagtgttttt
    13081 aactgttgta aaaacatgtt gctctttaga gctagtattg tatccaagtt ttaaatactc
    13141 ttctttttgc tgtaatttcc agttgttgag gatagaaaga gtctcagtat ctaaagagat
    13201 aactcgtcta gaagctcttg ttttcggaga ttgaattata actttaccaa attcatctac
    13261 agcaagagtt ttattgacat gtagttcttt attaaacgtg tctatgtctt tccactgtaa
    13321 agcgagtact tcactctttc tcatacctgt gtatgccaat aatctgaaga aggcgtattg
    13381 tttcatgtta ccaaaatctt taaaagcatc cagtaaatta ttaagctcct cttttgagta
    13441 aaatttacga tttttctcat actctttttt tcgtggaaga agtgtttttt ccataggatt
    13501 attgtcaata tattgcattg caaccccgta gcgtaaaatt tgtgcagtct gttttctcat
    13561 ataatcgtaa ctttcatatt tactgtgcca ttcatctacc actttttgac aatatgggac
    13621 agagatattc gatagtttaa gcttaccaaa tgctggaagt atttgattat ttgcgtatct
    13681 tactgagatt gcgattgttg aagctttaac actggtacgc tgatgagtta tccattcatt
    13741 atacaggtct tcaaatgtca tatcaggtgc tttcttttta acaccgtata atatttgatg
    13801 tctgtactcg tcataatctt tttgagcttc ttgtttgcta ctaaaacctc ttctcgtagt
    13861 atttattttt tcaccagttc gtggatcggt ggcaatgtgt cctttataca tccacgccac
    13921 ttgaccgttt ttctttttgt actttgatat ttttgccatg aaatcatctc ctcttagttt
    13981 acaaggagta cttaccacta gatggtatca cctcccttca tttttttctt agtgtatttt
    14041 ttcccaattg atgaaatcgt ttgttttagc ttgtttatat tatcaataat ttcgtttaca
    14101 gcttcttcgg gataattcat gtcttccact tgactatgta tcccttcatc tatcatttct
    14161 tctagttgtt cctctatact gctagtgagc agaagaatgt tattgtcaaa ttcttctata
    14221 atagcattga tgaataattc ttctattttt aagatattga aaagactaat tttttcattt
    14281 ttaactttat tacaagtctt gttagcaata aattcttgca tctctggttc taatttggaa
    14341 tatctatcca gccatgtatc taaattagag tgtactggtg ggaactgttg acgaagatgc
    14401 tggaacaggt ttttatctgg aaatgttata ccatctagta caatgctatc tctataaaga
    14461 ataattccca aaatatattc ttctacattg ccacaaacta actcctctat actcatattt
    14521 cccaaatcag caattcggga taatcgctct ggtattggta aataggttcc cttttcccat
    14581 tcgtagatag tttttttgtc aatcaattga ccattgttaa tcataagacc aaatgctggt
    14641 tgtgttaatt ttttactctt tcttatagct tttatttttg ttccgagtat cttttttcgt
    14701 tctaaataat ttattggtgg acgctttatc atattgacca cacctcctaa aattagtata
    14761 cctcaaaaag ggggtgttgt aaataaattc acacaacata ttgacacccc ttttgtatcg
    14821 aggtattatg tgtatgtcga tgaactgata atcatttttt atttgattaa tcggttaata
    14881 acaacaaaat acttttattt tgatcaaata atacgaagat gattcttaga aaatttgttc
    14941 aagtaatcac gaaaggaggg ggaaatatgt ctctacaaat cattgatagc attgtttcta
    15001 tacaagttga acaacaagtg aaagagataa ttacagtgct aaagcctatg ctagaagaca
    15061 ttatttctgg tgttactcta gagtatatgg actatcagca ggcaagtgaa tatctaggtg
    15121 tatcagtagg aacgattaga aaatatgttt ctcaatacgg gttacctgtt ataaaaattg
    15181 atacggtcat tagattcaaa aaatctgata ttgatagttt catggaacag tataaaggtt
    15241 gagtaaacaa ataagactaa aacatatagt aataaagagt ttgtgtttat tatctgtatg
    15301 ttttagtctt taacagaatt ttataaggag gaaacacaaa tggaaacatc aatgattaat
    15361 attccaattt atgtagatca agaaaaatta ttagagaagc cagttacaaa aaaagaaaaa
    15421 actgatgtaa gagataggtg tttagataac tatagagaag ttacaagaga tgagtttgta
    15481 gagatactaa attcatcaca atcatttatt ccaagcaaga caaagaataa aggcaatgac
    15541 aaggagagtt ttgtggaaac cagagtaatt attctggatg tcgataacac tgttaaagat
    15601 gagaagggga aggtagtaga tttgagtaaa gatgatagta gatacttaag cgtagaaaag
    15661 gttttatcta tcaatttagt tcataattct gcgtttgcga tacaaaaaag tattcggtac
    15721 tctgaaaatc tagaaaaata caagattgtc tttctattaa aagaagcaat tactgactat
    15781 ggtaaaatgg ttgctgttta cgaatacttg aaaaagaaca tccccgggtg tgatgacaat
    15841 gtaaatgctt cgaatagaat gttttttgga ggatataagt cagatgcagt gataataatt
    15901 aatgaagaca acatgttaga tattactgag cttccgattg attttaccat gacatgttct
    15961 aaaaattgtc aaagtgattc agggagagag tatgaactag tcaatgaaac cgattttgtt
    16021 aagttgataa aaaatgacga caaagaagaa atgaaacagt ggtttaaaga ctgcctgttt
    16081 gattcctccg acatgtcttt taatgagatt tatgaaagat tgttaaccat tgatatgaat
    16141 aaattactta agtcaagtaa aaacttaaga tgtctgtttc acaacgatca taatccctct
    16201 gctagtattt ttaccagtaa aactgggcat tccatttttt attgtcattc cagtaattgt
    16261 gatatcagtg aaaattttat tggagttgtg atgctaattt taaagaaaga cagtcgggta
    16321 gaaacattta attggctatt agagaaccta gacttgtatc ctacacattt tcgaaaattg
    16381 aaagaggaat caaaagtact ttttgacact cttgaatcaa tgaaagacaa acttttcagg
    16441 acaataagga attatctaga ggaaatgcga cgtgtctatg atgtcttgct ttctgatgtt
    16501 aatttttttg atgatagtaa ggaaagtgtg acttgtattt tgtcggggga acaattagct
    16561 aaaaaaatga gttcttacta tggtaaaact tatgacatag ataaatgtaa taagttacta
    16621 tcacttatga cttttatagg attgataaaa aaattagatg atgaagatgt tcctttgaag
    16681 atgctcgaat acctaaatag gttaaaggaa gagaaagcgt taggaaactt taatcataag
    16741 agaagcaatg tctatcgatt agacgttttt aatgcaactt ctgtgattca aaaattgaat
    16801 gaagatatcc ttccacttct ggaaaaaatt cattttacat atcaatattt ttcttatgaa
    16861 tgggttaaga tatgttttaa cgaaaaagag gctaaaagag tatttcctca aaattcccga
    16921 tcagaactca gcaaggaaaa acaattgatt ttagatgaag cacatgaagt tttacagggc
    16981 ttatcgtggc aagggacata tctaatgaca gagaaagaac ttattaacac tgtttgtgaa
    17041 cgcctacgta atgttgggga taacagcgtg aaaaatgctt taataaaaaa cagaggctac
    17101 tttgttaaac aaggattcac tcttgaacga gcttctaagg atattaagaa actatacaat
    17161 gtgaaagcaa gtagtagttt tttaatatat ttagcgaaag gggcagggat agatgaaaca
    17221 ataaaagaat tgatattatc aggagctggg atagttcctc tgtatcaatt tacaagtgta
    17281 acaagagatg atggacggag agtaaggaag agaaaagacc caattgtatt aaactagaca
    17341 ggttatccat agctcttaat cttatttcat ctcaatacac ccaccgttaa agttcggtgg
    17401 gttatttatt tttcaatctt tcaatagctg attgcgttca ttttcttgcc tttttaatag
    17461 cttcatatag tcaattgttc gaactgcatc ttcagtgcta agagaacgat agagagccaa
    17521 aaactcttcc tcataatcta cgacaacatt gtctagaaaa tggcttacag gggttcctag
    17581 ggctctagaa agagctgtta agctatcgag gttaggctct gttacagaat tttcccatct
    17641 tgctactgcc tgttgtgtag agaagatttt ttcagctaat tctttttgtg tccacccgag
    17701 agcttttcgc ttttctctaa tgaccttcgc atattgtttc tgtttcatta atacaccccc
    17761 atctcagtat agtacaacaa gttgttgtat ttgtaatggt atttgaagtt ttttatattt
    17821 tatgagttga aatacatcat attgttgtat ataataaaac aggaggggag aatcatgaaa
    17881 aaagtaagta atctcagccg atacagaaaa gaaaaaggac tttctcaaac ggagttagcc
    17941 aaaaaaatga atgttacaca gcaatgtgta agttcgtggc aaacagggag aacgattcct
    18001 aaaccctatc aaatgaaaat gctttctgag atattaagtg taccaataaa cgagctgttt
    18061 tctgatgtgt ttaataaagt aaacagttaa ctattaagca attgaatgat tttaggagga
    18121 gaagacatgt attgtagaaa atgtggtaaa gaaataacag atgaaagtaa gtattgccag
    18181 ttttgcggag cattacaaga aacagatgag acaactggag cagacgaagt aaagaaaagt
    18241 actgagcaga catttagtga aatgaaggaa caaatcgtac atggacaaga gacagggaag
    18301 ctgtatttga ttattggttg ggtttctatg gtagtatcgt tagcatttat tcctgttgta
    18361 tttggtgctg ttgctgttat tatgggctac ttatatcgag aaacagatga gaaaatgggt
    18421 acgattttga tgatagcagg ggtagctgga gcaatctttg gtgtactttt agggatgtca
    18481 atgtactgat aagagggata agagcaaatt attttggaga taggagataa aaagttgaat
    18541 aaagtaaata tgaacaagaa gtttgtcatt ataggtatcg ttgtcttggc tattataggt
    18601 atcatttttt caaaaatgac aggtacatct ggattagaga agtcatctaa accattggtg
    18661 acacagattt tacaagaaca gtacaactta aataggtcat gtgatgatgt gacgattact
    18721 catgaaaatg gagataacac ataccgggct aaagctacgt tagacaatgg aagtgctatc
    18781 aatataaatg tcgagtacta tccaaagaaa gaccgtgtat atgtagagat accatatact
    18841 gaggtgttaa tgttaaattg atttaaatgt attatgtata ccgtccaaac aggaactcct
    18901 aaaggtgtaa gcttactaag gagttttttt gattccc
//
LOCUS       DAEGIQ010000049        18805 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000049 DAEGIQ010000000
VERSION     DAEGIQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.049"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="KMT45_001557"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="KMT45_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBE7005023.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1692..2711)
                     /locus_tag="KMT45_001558"
     CDS             complement(1692..2711)
                     /locus_tag="KMT45_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7005024.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2733..3740)
                     /locus_tag="KMT45_001559"
     CDS             complement(2733..3740)
                     /locus_tag="KMT45_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBE7005025.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="KMT45_001560"
     CDS             complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="KMT45_001560"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBE7005026.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQTGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4391..6685)
                     /locus_tag="KMT45_001561"
     CDS             complement(4391..6685)
                     /locus_tag="KMT45_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBE7005027.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6945..9110
                     /locus_tag="KMT45_001562"
     CDS             6945..9110
                     /locus_tag="KMT45_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBE7005028.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9414..10247
                     /locus_tag="KMT45_001563"
     CDS             9414..10247
                     /locus_tag="KMT45_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBE7005029.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10349..12049)
                     /locus_tag="KMT45_001564"
     CDS             complement(10349..12049)
                     /locus_tag="KMT45_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005030.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12556..13716
                     /locus_tag="KMT45_001565"
     CDS             12556..13716
                     /locus_tag="KMT45_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBE7005031.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13732..14175
                     /locus_tag="KMT45_001566"
     CDS             13732..14175
                     /locus_tag="KMT45_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7005032.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14658..15188)
                     /locus_tag="KMT45_001567"
     CDS             complement(14658..15188)
                     /locus_tag="KMT45_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBE7005033.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15293..15844
                     /locus_tag="KMT45_001568"
     CDS             15293..15844
                     /locus_tag="KMT45_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBE7005034.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15997..16470)
                     /locus_tag="KMT45_001569"
     CDS             complement(15997..16470)
                     /locus_tag="KMT45_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBE7005035.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16608..18080
                     /locus_tag="KMT45_001570"
     CDS             16608..18080
                     /locus_tag="KMT45_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBE7005036.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18077..18787
                     /locus_tag="KMT45_001571"
     CDS             18077..18787
                     /locus_tag="KMT45_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBE7005037.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5760 a         3398 c         3884 g         5763 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat ttttgtgaat
     1501 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
     1561 agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac aatgtgttag
     1621 aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag cgtatttagt
     1681 gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt gatcaagcga
     1741 tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc gcgcagcttt
     1801 tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag gtaagggtgt
     1861 gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc tggaatgaca
     1921 tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc aatgaccacc
     1981 aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc ctccggatgc
     2041 tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt ttgataaccg
     2101 aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag gatattctta
     2161 tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt atcattatct
     2221 acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa agggatgtgg
     2281 ttttggtata aataatcaat gacagtatct tgactatctg aatacactag aataaaacca
     2341 tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc aaaattttta
     2401 gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat tgcttccatg
     2461 atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg taagattacg
     2521 ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg aacataacct
     2581 agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact aggatgatcg
     2641 ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac atcttttacg
     2701 gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg ataaatcgtc
     2761 tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg gaaatgaacg
     2821 atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat ctgttttcca
     2881 ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact tttattagaa
     2941 gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga agttttggaa
     3001 agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca tgcaaggatc
     3061 tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt tgtccaattt
     3121 ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac cgttaactga
     3181 tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg gttggtcgga
     3241 ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc gttttttgtt
     3301 aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc atcttttaag
     3361 taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca aaattggaaa
     3421 gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc caatctatgc
     3481 tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc tccaaaaaag
     3541 cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt ttctgatttt
     3601 ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag aagaacgact
     3661 tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac tggtacgacg
     3721 gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga tcgtaagaaa
     3781 agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc ccacgccaat
     3841 tggttgagcg ccacttgaga ggatggcttg gataccggtt tgcgcgtctt cgatcccgat
     3901 acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat ctggcgcagg
     3961 ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg taagacccat
     4021 tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct tgattttttc
     4081 ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg gttcgatcgt
     4141 ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa attcttcctt
     4201 tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt cttctcggct
     4261 gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt cctgaccaag
     4321 ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt ctaaatcaaa
     4381 caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga cgatccgcta
     4441 aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat aactcgaaag
     4501 taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg atatggaaag
     4561 aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca tgggcagttt
     4621 tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa gtgatgtgca
     4681 agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt cgctggtaca
     4741 tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg atacttggcg
     4801 aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt tgcttttctt
     4861 cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc tgcttgatga
     4921 agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga tcgcttgtgg
     4981 gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg acaaagattc
     5041 ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat ttggctagtt
     5101 cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt tctcgcgtgt
     5161 aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata ttgttctcat
     5221 attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt gaaaaatgga
     5281 cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca tttttcaaat
     5341 attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc gcgccatttc
     5401 ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca gtgaaggtga
     5461 ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt gcttgcggca
     5521 gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct aaagcaagat
     5581 aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat ttttctcctg
     5641 tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta gagaatagtt
     5701 ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg accacatcgg
     5761 caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg atataattgt
     5821 ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct ggaatatctc
     5881 tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga gataattctg
     5941 cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg actgattttc
     6001 cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca aagttgtttg
     6061 gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca atctcattcc
     6121 aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat tttgatacga
     6181 gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa gctgattctt
     6241 ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta gaagtagtga
     6301 ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa aagtcttctg
     6361 gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc gcgataaagt
     6421 tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac cagccgacac
     6481 gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg tcccctgagt
     6541 aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt gttaaagatt
     6601 cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat ggatcaatag
     6661 caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt atacgttcat
     6721 aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat agaaaaaagc
     6781 ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt ataggtttta
     6841 tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa aacgcacgct
     6901 ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa aagatattta
     6961 gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca gttatgccgg
     7021 cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct gattggacgc
     7081 cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt ggtaatcttc
     7141 atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca ggcggagcat
     7201 ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc tttggtgtca
     7261 caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt acgaagatca
     7321 tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg ggagtattcg
     7381 tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat aattatagaa
     7441 aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt gttgttatct
     7501 tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta caggctggaa
     7561 tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt ctagcaccgt
     7621 ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat atgctgacga
     7681 ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc gcacaggcgg
     7741 ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat ttagtgaatc
     7801 ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg acgccggctc
     7861 gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg gcttttgcga
     7921 tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat ttttcagcag
     7981 cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt atgtttgctg
     8041 ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg atggcagata
     8101 tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg acaccgttag
     8161 ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg atcgcttttg
     8221 gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt gctacaccgg
     8281 gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac agcggaacag
     8341 gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa gaaaatatca
     8401 aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga gaaaaagtag
     8461 ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa gacaatggag
     8521 tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa gatctgctgc
     8581 aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga caagcaggta
     8641 gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga gaagtgattc
     8701 ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac ggctttgctg
     8761 ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca agtattttcc
     8821 ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg atccatatgg
     8881 gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa gaaggccaag
     8941 aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa aacgaagaaa
     9001 aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta gtgatcaagc
     9061 aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa ggagaggttg
     9121 tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc tcctcctatt
     9181 tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt agtggagagg
     9241 tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa tagctcctgg
     9301 tccattgagc ttattactga ggagttagca ggagaacact atttgctgga gaggttgtga
     9361 caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa ccaatgaaat
     9421 tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa ttgcaagaat
     9481 tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa atcaaccaat
     9541 tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg gtaaagaatc
     9601 aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta aaggaacacg
     9661 gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa tatgaagaag
     9721 gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc tcccaatttc
     9781 aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag atagatgatc
     9841 aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa agtggctttt
     9901 attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca ctttttttcc
     9961 taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga gaaagttgcc
    10021 ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct actgtggaaa
    10081 agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt attttcgtcc
    10141 ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat agtccaatca
    10201 tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa atataacata
    10261 taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt tggagagttt
    10321 tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat ctatgataaa
    10381 taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg aaatagaaat
    10441 agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc ttcacgattg
    10501 gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc tgtagcacag
    10561 tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt ttccacttga
    10621 ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct agtatttctt
    10681 cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga tagtcaggtg
    10741 tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat gttttccttg
    10801 cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca ttttcccact
    10861 tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga atgccaaata
    10921 tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg gcaaggtaaa
    10981 gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc aaactaataa
    11041 aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa agaaaaaaga
    11101 aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt tgaaagaaaa
    11161 taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa tggagccaat
    11221 aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag ggaggaagaa
    11281 tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc agaacaaaat
    11341 aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc tggttaaaaa
    11401 agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac agtaaatgga
    11461 tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt ttatgaagga
    11521 cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag ctagtgaatc
    11581 cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg gctactggta
    11641 cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa aatcctgaaa
    11701 taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct gcaagcaaca
    11761 cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg atcctgccac
    11821 tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta atttgttgcg
    11881 cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt agtttatgtg
    11941 tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa aatgggccat
    12001 attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata tatcctatgc
    12061 gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta caagaaaaac
    12121 ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc tccttgtttt
    12181 gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct ctcggtaaac
    12241 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
    12301 ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
    12361 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
    12421 gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg
    12481 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
    12541 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
    12601 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
    12661 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
    12721 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
    12781 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
    12841 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
    12901 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
    12961 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
    13021 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
    13081 actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
    13141 gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg
    13201 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
    13261 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
    13321 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
    13381 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
    13441 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
    13501 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
    13561 ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
    13621 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
    13681 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
    13741 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct
    13801 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
    13861 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
    13921 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
    13981 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
    14041 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
    14101 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
    14161 aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg
    14221 gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
    14281 tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14341 gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    14401 tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag cctagaaaat
    14461 atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct gatttcacaa
    14521 cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    14581 catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc tgatttcaca
    14641 acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta ttttccgcta
    14701 cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc attgtctata
    14761 cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt tggataagtt
    14821 tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa agtaacagtg
    14881 atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag tttcttttca
    14941 tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt gattttgaat
    15001 acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg gcctttgacc
    15061 attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca ttcatattcc
    15121 caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa ttctttaaat
    15181 tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac atgttttcga
    15241 ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac tgatggcaag
    15301 aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga tgacaggata
    15361 tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa aacatagtca
    15421 gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc aagtagagat
    15481 cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga aggaacaatt
    15541 ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg aatttgttct
    15601 gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta ttcagcagca
    15661 gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa cgatctttcc
    15721 tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc atgcgatcaa
    15781 tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta accgaaaaaa
    15841 ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa acgcagatca
    15901 ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt agcatctact
    15961 tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta acaactgttc
    16021 gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg tctcgttgtc
    16081 atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta aaatcgtata
    16141 gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac ggacatggtg
    16201 cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa atccgtcatc
    16261 taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt ttctaggaca
    16321 attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc ttaattgacg
    16381 tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt gatcattttc
    16441 caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc tcttgcttcc
    16501 cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg aatttaggac
    16561 taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg agtaacaacc
    16621 aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt gtcttattaa
    16681 ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt cctacattga
    16741 tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt ttcttgctag
    16801 tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt tcaagaaatt
    16861 tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt acagcaccta
    16921 attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga gtatcgatgc
    16981 cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga tcagcaatgg
    17041 ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta tctggttata
    17101 tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc gttgttcttg
    17161 tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat cctagcattg
    17221 atattttatc cgctatttta tcaacgattg gattcggtag tttactttac ggattttcta
    17281 gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt gttggtgtga
    17341 ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc ttggaacttc
    17401 gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta acaaatatgg
    17461 cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt ggagaatcag
    17521 cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct atgatgcctg
    17581 tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca gggatgttca
    17641 ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca gttttatata
    17701 tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg ccggtaacga
    17761 cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg gtaaataata
    17821 cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg ttgacgaatg
    17881 tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta gcttataaag
    17941 atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta gcgatcatct
    18001 ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct gttctaatga
    18061 aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg cttttgcttt
    18121 aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt tcggactaat
    18181 cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg gaatggaaaa
    18241 agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag gagcagatat
    18301 gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc gaacaaatga
    18361 aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag tagaaaagac
    18421 agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa acgacatgta
    18481 taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag tgaatacatt
    18541 caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt tagctgaatt
    18601 agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc aagacggtat
    18661 ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac aacgtacgaa
    18721 agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag aagcaaaagg
    18781 cgaataatat tttgaacacc atgta
//
LOCUS       DAEGIQ010000050        18107 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000050 DAEGIQ010000000
VERSION     DAEGIQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.050"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(164..388)
                     /locus_tag="KMT45_001572"
     CDS             complement(164..388)
                     /locus_tag="KMT45_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBE7005038.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="KMT45_001573"
     CDS             complement(586..2166)
                     /locus_tag="KMT45_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBE7005039.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="KMT45_001574"
     CDS             complement(2567..3943)
                     /locus_tag="KMT45_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBE7005040.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="KMT45_001575"
     CDS             complement(4071..5189)
                     /locus_tag="KMT45_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE7005041.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="KMT45_001576"
     CDS             5331..5765
                     /locus_tag="KMT45_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7005042.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="KMT45_001577"
     CDS             complement(5843..6376)
                     /locus_tag="KMT45_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBE7005043.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="KMT45_001578"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="KMT45_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBE7005044.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="KMT45_001579"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="KMT45_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBE7005045.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="KMT45_001580"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="KMT45_001580"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBE7005046.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="KMT45_001581"
     CDS             complement(10101..10679)
                     /locus_tag="KMT45_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBE7005047.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="KMT45_001582"
     CDS             11001..11471
                     /locus_tag="KMT45_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBE7005048.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="KMT45_001583"
     CDS             complement(11579..12805)
                     /locus_tag="KMT45_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBE7005049.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="KMT45_001584"
     CDS             complement(13559..13759)
                     /locus_tag="KMT45_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBE7005050.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="KMT45_001585"
     CDS             complement(13893..14501)
                     /locus_tag="KMT45_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBE7005051.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="KMT45_001586"
     CDS             complement(14586..15056)
                     /locus_tag="KMT45_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBE7005052.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="KMT45_001587"
     CDS             complement(15104..16018)
                     /locus_tag="KMT45_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBE7005053.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="KMT45_001588"
     CDS             16164..16967
                     /locus_tag="KMT45_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBE7005054.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="KMT45_001589"
     CDS             17145..18092
                     /locus_tag="KMT45_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBE7005055.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5438 a         3631 c         2964 g         6074 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaata
//
LOCUS       DAEGIQ010000051        17748 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000051 DAEGIQ010000000
VERSION     DAEGIQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.051"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            37..849
                     /locus_tag="KMT45_001590"
     CDS             37..849
                     /locus_tag="KMT45_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBE7005056.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            846..1646
                     /locus_tag="KMT45_001591"
     CDS             846..1646
                     /locus_tag="KMT45_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBE7005057.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1807..3075
                     /gene="rseP"
                     /locus_tag="KMT45_001592"
     CDS             1807..3075
                     /gene="rseP"
                     /locus_tag="KMT45_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBE7005058.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3143..4852
                     /locus_tag="KMT45_001593"
     CDS             3143..4852
                     /locus_tag="KMT45_001593"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBE7005059.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5064..9416
                     /locus_tag="KMT45_001594"
     CDS             5064..9416
                     /locus_tag="KMT45_001594"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBE7005060.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9560..10033
                     /gene="rimP"
                     /locus_tag="KMT45_001595"
     CDS             9560..10033
                     /gene="rimP"
                     /locus_tag="KMT45_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBE7005061.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10057..11232
                     /gene="nusA"
                     /locus_tag="KMT45_001596"
     CDS             10057..11232
                     /gene="nusA"
                     /locus_tag="KMT45_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBE7005062.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11254..11547
                     /locus_tag="KMT45_001597"
     CDS             11254..11547
                     /locus_tag="KMT45_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBE7005063.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11544..11855
                     /locus_tag="KMT45_001598"
     CDS             11544..11855
                     /locus_tag="KMT45_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBE7005064.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11868..14174
                     /gene="infB"
                     /locus_tag="KMT45_001599"
     CDS             11868..14174
                     /gene="infB"
                     /locus_tag="KMT45_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBE7005065.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14201..14548
                     /gene="rbfA"
                     /locus_tag="KMT45_001600"
     CDS             14201..14548
                     /gene="rbfA"
                     /locus_tag="KMT45_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBE7005066.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14656..14877)
                     /locus_tag="KMT45_001601"
     CDS             complement(14656..14877)
                     /locus_tag="KMT45_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7005067.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15325..17688
                     /locus_tag="KMT45_001602"
     CDS             15325..17688
                     /locus_tag="KMT45_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBE7005068.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6050 a         3148 c         3912 g         4638 t
ORIGIN      
        1 gatagtggga atcttttaag tataggagga aaaagaatgt tacgtttttt tccgcaaaaa
       61 aacaaatata ttcaagaaaa aagcgaattt caatttaata ccgatggacc aattcctcag
      121 cacattgcca tcatcatgga cggcaatggt cgttgggcgc aaaaccgtcg tttgccacga
      181 gttgctggcc ataaagaagg catggaaaca gtaaagaaag tcacaaaaaa agcatcaagg
      241 cttggagtga aagtgttgac gctttatgcc ttttcgactg aaaactggaa gcgaccaaaa
      301 gatgaagtaa gttttctcat gcaactacct gtcgacttct ttgatacgtt cgtaccagaa
      361 ttgattaaag aaaatgtcaa agttcatgtc atgggttatg aaaacgtatt gccagaacat
      421 acgcaagatg ctgtaagacg tgcaattgag caaacaaaaa acaatactgg aatggtattg
      481 aattttgctt tgaattatgg aagtagagca gagatcgtga ctgctgtcca agaaattgcc
      541 gaagaagtag caaaaggaga aatacacgca gaagaaatcg atgatgaatt gattgctaag
      601 catttaatga caggtttctt gcctaaagag ttacaagatc ctgagctgat gatccgtaca
      661 agtggagaag agcgaattag taattttctg ttatggcaga tcgcatatag tgagctgtat
      721 tttacaaaag cgctttggcc tgattttgat ggtgcgcatc ttgaagaagc aatcgcctct
      781 tatcaaaatc gagaccgtcg attcggcggt gtaaaaaaaa cagaagatga gaaaggggat
      841 cagtcatgaa acaacgtgtg atcacagcag ctgtggcgct agtcttgttt attccgatta
      901 tatggatcgg aggcatagcg gttgagctta cagcagcaat cttagcggta gtaggagttt
      961 acgagctgtt ccggatgaaa ggattgacac tcttaagttt tgaaggagtt ttatccgccg
     1021 ttggtgcagt gttacttgtt ttgccaaaag aaagatggtt tttctttctt ccagagaatg
     1081 taagtacatt tatgttattt tatcttatag ttatgattct tttaggaaca tcagttattt
     1141 ctaaaaatac gtatacgatt gatgaagcag gttttcctgt gcttgtcagc ttgtatgtgg
     1201 gaataggttt ccagaatttt gtcaatgcac gaacagaaag cttggctgtt ttgatatttg
     1261 gtctatttat tgtttgggca actgatatcg gagcatatat ggttggtcga cgttatggtc
     1321 agcataagtt aagtcctgat atttcgccca acaaaacgat tgaaggtgcg ctcggtggta
     1381 tcggtagtgc tgttgtagtc gcatttcttt atttattgct atacccagca aaagaactat
     1441 tcaatcatgg tatgtttgtc atgcttctga tgacgattat tttttcagca gtcggacaat
     1501 ttggtgattt ggtcgaatct gcaatcaaac gtcattatga tgtgaaagac tcgggaaata
     1561 tcttaccagg ccatggtgga atcttagacc gtttcgacag tttgctgttt gtatttccta
     1621 tcatgcattt atttggcatt atttagtaag tatacctgcg tttctttgtg aatttcataa
     1681 aagacgcagg tttttatttt tcattatatt gatactattg gtgtctttgg tatactatgg
     1741 taaaattata gtgttttatc gttgttttat aaaaaacaca aaaaacaagt aaaggagaaa
     1801 aaagctatga aaacgattct gacatttatc atcgtttttg gtatattagt gattgttcat
     1861 gagtttggtc atttcttctt tgcgaaacga tcaggaatcc tcgtccgcga atttgctatc
     1921 ggtatgggtc cgaaaattta tgggcatcaa gcaaaagacg ggacaacata tacgcttcgc
     1981 ctattaccta ttggcggtta tgttcgaatg gcaggcaatg gggatgatga aacagaaatg
     2041 gcaccaggaa tgcctctttc tcttttacta aattctgacg gcatcgtaga gaagataaat
     2101 ttaagtaaga aaatccaatt gacaaatgct atcccgatgg aattgagccg ttatgattta
     2161 gaagatgagc tgactatcac tggttatgtg aatggagacg aaacagaagt agtaacctac
     2221 cctgttgatc acgatgcgac gattattgaa aacgacggaa cagaaatcag aattgcgcca
     2281 aaagacgtac agttccaatc ggctaaattg tggcagcgta tgctgaccaa ctttgctgga
     2341 cccatgaata actttatttt ggcaatcgtg ttattcatca ttctggcatt tatgcaaggt
     2401 ggtgttcaag tgacgaacac taatcgcgta ggagagatca tgcctaatgg agctgcggca
     2461 gaagcaggct taaaagaaaa tgatgaggta gtcagtgtgg acggaaaaga aatccattca
     2521 tggaatgatc tgacaacagt cattacgaaa aatccaggta aaacattaga ctttaagatc
     2581 gaacgagaag gtcaggtgca atctgtcgac gtgacaccga aatctgtaga gtcaaatggc
     2641 gaaaaagttg gccaacttgg tataaaagcc ccaatgaata caggttttat ggataagatc
     2701 attggaggga cgcgtcaagc tttcagcggt tcattggaaa ttttcaaagc cttaggatca
     2761 ctatttacag gtttcagttt agataaatta ggcggacctg tcatgatgta ccaattgtct
     2821 tcagaagcag caaatcaagg tgtaacaact gttattagtt tgatggcgct tctttcaatg
     2881 aatctcggaa tcgtcaatct gcttccgatt cctgccttag atggcgggaa attagtcttg
     2941 aatatttttg aagggattcg cgggaaacca ttaagtcaag aaaaagaagg aatattgaca
     3001 ttagctggct ttggattttt aatgttgttg atggtgctag ttacatggaa cgatattcaa
     3061 cgattctttt tctagaaaaa gttgacaaat agggtataat aacaaacgat aactattttg
     3121 aggaaataaa ggagtatata gaatgagaca atcaaaaatg ttgattccaa cattacgcga
     3181 agttccaaat gatgcagaag tattaagcca tcaaatcttg ctaagagctg gatatatccg
     3241 ccaagtatca gcgggtattt attcatatct gccattagca aatcgtgtat tggaaaaact
     3301 gaaacgcatt atgcgggaag aatttgaaaa aatcggagcg gtagaaatgc tgatgcctgc
     3361 catcttgcca gcagaacttt gggaagaatc cgggcgttac gaaacatatg gaccaaactt
     3421 gtatcgtttc aaagaccgca atgatcgaaa aatgatcctt ggaccaactc acgaagaaac
     3481 ttttactgat ttgatgcgta atgaaatcaa ttcatataag aaattacctt taaatcttta
     3541 tcaaatccaa cctaaatatc gtgacgaaaa acgacctcgc ttcggtttgc ttcgcggaag
     3601 agagtttatc atgaaagatg catattcttt ccatgcaacg gaagaaagtt tggacgagac
     3661 atataaagat tacgaaaaag cctataccga agtattcaag cgttgtggct tagaatttcg
     3721 ctcgatcatc ggagacggcg gcgcaatggg cgggaaagac tcaaaggaat tcatggcgat
     3781 ctcagaaatc ggggaggata cgatctgtta ttcgactgaa agtgattatg ctgcaaactt
     3841 ggagatggct acaagctact atgtatcaaa aaaatcgcat gaaacacagc ttgaattgga
     3901 gaaagtagct acacctaacg taaaatcaat tgacgaagta gcagatttct tttctgttga
     3961 acctcaaaaa atcatcaagt ctgtattgtt cattgcggat gaacaacctg tccttgtttt
     4021 ggtacgtgga gaccatgaag tcaatgacgt aaaactgaaa aactttttat ctgctgactt
     4081 tttagaagaa gcaacagaag aagaagcaca aaaatatcta ggtgctgagt ttggttctgt
     4141 cggaccagtc ggtgtttcag atgacgtaaa agtatatgca gaccgttatg tccaagattt
     4201 agcgaatgcc attactggag cgaacgaaac aggattccac tataaaaatg tgaatccaaa
     4261 acgcgatttc actgtgcttt cttatgaaga tcttcgtttt gtacaagaag gagatccttc
     4321 accagacaac aacggtgttc ttgcctttac tcgaggaatc gaaatcggac atatattcaa
     4381 aatcggcact cgttacagtg aatctatggg agcaactgta ctagacgaaa atggacgaga
     4441 aaaatttgtg attatgggct gttatggtat cggtgtaagt cgtttattat cagcaatcgt
     4501 agaacaacat tcagatgaac aaggaatcaa ttggccaaaa ggaattgcgc catttgatct
     4561 gcatatcgtc caaatgaatc taaaagacga ataccaaaca agcctgacga atgaactaga
     4621 agaagcgatg acaaaagctg gctatgaagt actggttgat gacagaaatg aacgcgcagg
     4681 agtgaaattc gcggattctg atttgattgg ctgtccaatc agaatcactg tcggtaaaaa
     4741 agcagtggat ggaatcgtag aagtgaaaat caagaaaact ggcgaaatgg ttgaagtacg
     4801 taaagaagaa ttggcgaata ctttaccaat cctcttgaac caaggcgaat aataagcgtt
     4861 aaaacacaga aaataaaaat agctgaatca gctgtttttg acaatctgat gattcgcttt
     4921 taaaattgga actgcgattt gaatcgcagt tccaattttt taatgtaaaa ctcagaaaaa
     4981 acgagtatac taaaaggtac tgaaaaagaa gaggataaga tgcgatcaat tgcattgttt
     5041 ctcatcagaa agggcgtata tctttgtcag aaaaacgaga tttatttgaa aaactgttgg
     5101 aacaaatcca actagaggaa gcagaaaaaa atcatccact gcttactgct ggtgaaatgg
     5161 atagtgtagt ggtccatcgt aaatcgcgtt tatgggaatt tacgctacat ttttccaaaa
     5221 ttttgcctat tatgctatat cggtcattga ctcagcatct gactatcgct tttaaagata
     5281 ttgcacaagt caaactgaat atcttagcag atgaccagac attcgatgaa cagctcttgc
     5341 aagattactg ggcccaagct ttggaaaatc agcaatgcga tacaccacta gcacagcaag
     5401 tattgaaaac gcaggtacct gtgataaaag atcgtaaagt catcttgcca atcgacagta
     5461 caggagctat ttcttatttg aagcaacaat acttgccatt agtagaagag ttgttcgtca
     5521 gttacggatt cccaaaattt cgtatcgaac cagaagtgga tgagcagcaa gcggaacgtg
     5581 tattgaaact ttttgaagaa cgcaaacaag aacaggcaga agcctttatg aagcaagcag
     5641 ctgaaagtct agttgtccac gaacaaaaga aaaaggaaag aaaagagcaa tcgccagctc
     5701 ttgaaggacc catccagcta ggacgaaata ttccagccga tgaaccaacg acaccaatga
     5761 tcagtatcgt tgaagaagaa cgaagagtca cacttgaggg gtacgtattc gacaaagaag
     5821 tcagagaact acgttcaaaa cgcaagatct tgactttgaa aatcacagat tatacttcat
     5881 catttatcgt caaaaaattc tcgaacggcg aaaaagatga acaagtattt gatgcgatca
     5941 gtgtcggtag ctggttaaag gtacgaggaa gtattcaaga agatacgttt gtccgtgatt
     6001 tggtcatgaa tgcgcaagat atcatagaag tcaaacatac acctcgcaaa gattatgctc
     6061 ctgaagggga aaaacgagta gagcttcatg ttcatagcaa tatgagtacg atggatgcaa
     6121 caaacagcat ctctgatctt gtggcacaag caggaaaatg gggacaccga gctatcgcca
     6181 ttacagacca tggtggtgcg caagcctttc cggaagcaca tagtgctgga aaaaaagcag
     6241 gtgtgaaaat cttatacggc gtggaagcga atgtcgtaga tgatggtgtc ccaattgctt
     6301 ataatgatgc acatgaagca ttgagtgaag ccacatacgt cgtatttgac gtggaaacga
     6361 caggattgtc tgcagtatat gacacgatca tcgagctagc cgcagtcaaa atgtataaag
     6421 gaaatgtgat cgaaagcttt gatgaattta tcgatccagg acatccattg tcacgaacaa
     6481 cggtagatct gactggtatc acggatgaaa tggttcgagg gtcaaagtcg gaagaagaag
     6541 tattgcggat gttccttgaa ttttcaaaag atacaatttt ggtagcccac aacgcggctt
     6601 ttgatatggg gttcttgaat actagctacg caaggtatgg tattcctgaa gcggccaatc
     6661 cagtcattga tacgttggaa ttagctagat atctgtatcc tcaattcaaa cgtttcggat
     6721 taggtgtgtt atctaaaaag tttggtgtta gcttggaaca gcatcaccga gcaatctatg
     6781 atgctgaagc aactggtcac ttagcatgga tctttgtgaa agaggcaatg gacaatcata
     6841 acatgcttta tcatgatcag ctaaatgagc atatcggaga aggagattct tacaaaaggg
     6901 ctcgaccttt ccatgtaacg attttagcaa aaaatcaggc aggactgaaa gatttgttca
     6961 aactgatttc aatgtcgaat gtcgaatact tcgaacgcgt cccacgaata ccacgctccc
     7021 aattgaaaaa gatgcgtgaa aatctattga tcggctcagc ttgtgataag ggagaaatat
     7081 tcgaagccat gatgcaaaaa ggggtagaag aggcaagaaa ccgagcaaaa ttctatgatt
     7141 atattgaagt tatgccaaaa gcggtctatg cgcctttgat cgagcaagaa ttggtaaaaa
     7201 atgaacatga tttggaagaa attatccaaa atttggtaga aatcggaaaa agtttggata
     7261 aaatcgttgt tgcaacaggt aatgtacatt atctgaacga agaagatgcc atttaccgaa
     7321 aaattttgat caattctatg ggcggagcga atccgttgaa tcgtcatagt ttgccggatg
     7381 tccatttccg aacaaccgat gagatgctaa ctgcatttca ctttttaggg gaagaaacag
     7441 cgaaagagat cgtagtagaa aatacgaata aaatcgcaga tatctgcgaa gaagtgattc
     7501 cagtcaaaga tgaactttat acaccgaaga ttccaggttc agaagatgaa atatcagaat
     7561 tgagttacac aaaagcaaaa caaatgtacg gagatccgtt acctgagatc attcaaaaac
     7621 gattagaaaa agagttgaat tctattaatg gtaatggctt ctcggttatt tatctgatcg
     7681 cccaaaaact agtgcataaa agtaatgaag atggctatct agtaggttca cgtggctctg
     7741 ttggctctag ctttgttgcg acgatgacag gaatcactga agttaatcca ttagcccctc
     7801 attattattg tccagagtgc caatattccg agttttttga agatggtacg tatggttctg
     7861 gatttgacat gccagaaaaa caatgtccaa aatgtggtgc acgcttaaac aaagacggtc
     7921 atgatattcc atttgagaca tttttaggat tccatggaga caaagtgccc gatattgatt
     7981 tgaacttctc aggtgattat caagcagaag cccataacta tacgaaagta ttgtttggcg
     8041 aagactatgt ttaccgtgcc ggaacgattg gtacagtcgc cgacaaaaca gcatatggct
     8101 atgtaaaagg atacgaaagg gacaataatc tgcagtttcg aagtgcagaa gtcgacagat
     8161 tagcaaaagg cgctactgga gtcaaacgga caaccggcca gcatccaggg gggatcatcg
     8221 ttattcccga ttatatggat gtctatgatt ttacgccgat ccaatatcca gcagacgatc
     8281 aaaattcaga atggaaaacg actcattttg atttccactc gattcacgat aatgtattga
     8341 aattagatat tctagggcat gatgacccaa ctgttattcg gatgcttcaa gatttatcag
     8401 ggatcgaccc acaaacgatc cctacggatg acccggaagt gatgcggata tttgccggtc
     8461 ctgaagtact tggagtaagt caggaacaaa tttattccaa aacagggaca ttaggtattc
     8521 cagaattcgg cactcgtttt gttcgaggga tgttagaaga aacgcatcct acaacttttg
     8581 cagaactact gcaaatttcc ggactatctc atggtacaga cgtatggtta ggaaatgcgg
     8641 aagaattgat tcgacgagga gatgcgacac tggcggaagt aatcggctgt cgtgatgata
     8701 ttatggttta tttgatccat gctggattag atagcgggat ggcatttaaa atcatggaaa
     8761 ctgttcgtaa aggacaatgg aacaagatcc ctgatgaatt gcgagaaacc tatctatcgg
     8821 cgatgaaaga aaataatgtg cccgactggt atatcgattc ttgttcaaaa atcaaatata
     8881 tgtttccgaa agcccatgct gcagcttacg tattgatggc tcttcgtgtc gcttatttca
     8941 aggtttattt ccctattttg tattactgtg catacttttc tgtacgtgca gacgactttg
     9001 atctagtctc catgtgcaaa ggaaaagatg ctgtgaaaca agcaatgaaa gaaatcacgg
     9061 acaagggatt agatgcttcc gtaaaagaaa agaatcaact gacagtgctg gagttagcca
     9121 atgaaatgct ggaacgagga ttcaaatttg gaatgattga tttgtataaa tcggatgccg
     9181 ttaactttgt tattgaaggt gatacgttga tcgcaccatt tagagctgtc ccaagtttgg
     9241 gaaccaacgt ggcgaaacaa atcgttgaag caagaaaaga cggtccattt ttatccaaag
     9301 aagatcttgc cacccgcggt aaagtttcaa aaacattgat cgaatatatg aacgacaatg
     9361 gcgtattgaa agatctacca gatgaaaatc aattatcgtt attcgatatg ctataagcat
     9421 ctgttttcct gtctgcttgt aattttaagc agagtatggt atagttctta taggtaatga
     9481 tactaactgt gagtgagcgg atttttcgct cactcttttt aatggaatta cgcacttaat
     9541 ttttaaggag gcgaaacttt tgagtagcgt cgttgaaaca gtcacagaaa tggtgacacc
     9601 gattttagat gaacaaaatt ttgaacttgt cgaagtcgaa tttgtaaaag aaggaaaaag
     9661 ctggttctta cgagtattta ttgacaaaga aggcggtatc gatatcgaag aatgcgcctt
     9721 tgtcagtgag aaactgagtg aaaaacttga tgcagcagat ccagatccga ttcctcaagc
     9781 ttatttcctg gaggtatcct ccccgggagc agagcggcca ttgaaaaaag aaagcgacta
     9841 tgaaaaggca cttggtgatt atgtccatgt ctccttatac caaccagtag atggcgaaaa
     9901 acaatatgaa ggatttcttc aatcatttga tgctgaacag cttacattga aaatacgcat
     9961 aaaaacacga gaaaaagaat tgacttttga ccgaaaaaat attgcaaaag ctcgcttagc
    10021 tatccaattt taatcacgga gaggaatcag aaaaaaatga gtaaagaaat gttaaatgcg
    10081 ttagacgctt tagaagctga aaaagggatc tcaaaagaaa tcgtgatcga tgctttagaa
    10141 gcagcattgg tttctgcata caaacgtcac tatggccaag cacaaaacgt cgaagtggaa
    10201 tttgatcaga aaaaaggaaa gatccatgtc tatgcggtaa aagaagtaac ggaagaagtg
    10261 atggattcac agttggaagt ctcattgaaa gatgcacttt taatcaatcc agcatatgag
    10321 atcggtgata cgatccgttt tgaagtaaca ccaaaagatt ttggacggat cgctgcgcaa
    10381 acggctaaac aagttatttt acagcgtgta cgtgaagcag aaagaacgat tatttataac
    10441 gaattcagtg cttatgaaaa agacatcatg caaggaatcg tcgaacgcca ggataaacgt
    10501 tatatttatg tcaatcttgg taaaattgag gcagttcttt ctaaacaaga tcaaatgcca
    10561 aatgaatttt atcaaccaca tgatcgaatc aaagtttatg tttcccgagt agaaaatacg
    10621 tctaaaggtc cgcaagtatt tgttagccgc agccatccag acctattgcg tcgcttattt
    10681 gaacaagaag taccagaagt ctatgatgga atcgttgaaa ttgtcagcat cgcccgtgaa
    10741 gctggcgatc gttcaaaagt ggcagttcgc tcaaatgatc caaatatcga tgcagtcggt
    10801 acatgtgtcg ggccaaaggg acaacgagtt caagcaatcg tcaatgaatt aaaaggcgaa
    10861 aacatggata tcgtcgaatg gaatgaagat ccagctgtat tcatcgcaaa tgcattgaat
    10921 cctgcacaag tcatggatgt gatcttcgat gagacaaatc caaaggcctg cacagttgtc
    10981 gttccagact accaattatc gctagcaatc ggtaaacgcg gacaaaatgc acgtttagct
    11041 gctaaattga ctaaccataa aatcgatatc aaatcagaat ctgacatgac ggaattttat
    11101 gaaaatcaag aacaacaaaa agaaacagaa gaactgcatg atgaagcaat cgttcagtca
    11161 gatttaacag acgatgaata tgaaacaatt gcattcaaca atgaaacggt cgaagaaaaa
    11221 ccagaagctt aactttagta aggaggcaaa gagatgaaac aaaggaaaat tcctttacgc
    11281 aaatccgtgg tttcaggtga aatgaaacct aaaaaagaga tggtccgaat cacccgttca
    11341 aaagaagggg aagtatcgat cgatccaact ggaaaaatgc ctggacgagg agcgtatgtc
    11401 tcacttgagc cggaagaagt acaacaagct tgggataaaa aaattctcga tcgagtactc
    11461 gaaacaacat taacagatga gttttatcaa gaacttcttg actatgtcac tcaccaaaaa
    11521 gcacgtaaag agttatttgg aaaatgaata cagttaatcg gcaaaaagcc atgaatctca
    11581 tcggtctagc gatgcgcgcg ggaaaactaa tcacgggtga agaactgacc attgctgaaa
    11641 tccgcaaaca aaaagcaaaa ttggtgtttg tggcaaccga tgcaagtgag aatacaagaa
    11701 aaaagataaa agataagagt tcatattatg aagttccttg ctttgagcta ttttctgaag
    11761 cagaaatcac gcagatgatc ggaaaacctc gcaaggtatt cggaataatg gatgccggat
    11821 ttgcgaagaa gaccaaggag ctaattgaag gttaggaagg tgattgcatg ggcaacaaaa
    11881 gaatctatga attagcaaaa gaactcaaaa aatctagtaa agacgtcgtg gaaaaagcac
    11941 aacaacttgg catcgatgtg aaaaaccata tgggtgcgat tactagcagt gatgagaaga
    12001 aattgcaaca agcgttcaaa aacccaagca cgaaacaaca agctgcacaa caagcaagca
    12061 gaaaaccagc caatcaacag ccgaacgatc aacgtaaaaa agaaacaacg aacaatcaac
    12121 aaaaaaataa aaataaccgc agttatcaag atcgcggtca agggagcggt caagtgaatc
    12181 aaggaaaaaa accaacaaca aaccaaaata atacgggaaa taccagcagc caaaatcgtc
    12241 aaggaacgag ccaaggcggc aatcaaaacc gtactaacaa taacaacaaa ccaaacaata
    12301 acaatcgcgg caaatttaat aataataacc gtaatcgtta taataaaaaa ggtaaaaaag
    12361 gcaaacagca acaatcaact aaaccagctg taccaccacg caaattccgt gaattgccag
    12421 atgtattaga gtatactgaa ggtatgaacg tagcagatat tgcgaaaaaa attcatcgtg
    12481 agccagcaga aatcattaaa aaattattta tgcttggtgt catggtcaat caaaaccaag
    12541 cactggataa agatacgatt gaactgttag cgacggacta tggcatggaa ccacaagaaa
    12601 aagtccaagt tgacattgct gatatcgata aattcttcga ggcagaagaa gtcgatccag
    12661 ataaattagt ctcacgtccg ccagtagtaa cgattatggg acacgtagac cacgggaaaa
    12721 caaccttatt ggatacattg cgccattcac gtgtaacaag cggcgaagcc ggcggaatca
    12781 cacagcatat cggtgcctac cagatcgata ttgatggtaa accaatcact ttcttggata
    12841 caccaggaca tgcggccttt acaagtatgc gtgcacgtgg agcaagtatc acagatatca
    12901 cgatcttagt tgttgctgca gatgatggcg tcatgccaca aacagttgaa gcaatcaacc
    12961 atgccaaagc tgcaggcgtt ccaatcatcg tcgctgtaaa taaaattgat aaaccaggtg
    13021 caaatcctca gcatgttatg caagaattaa gtgagtatga attaattcct gaagcatggg
    13081 gtggagaaac gatttttgtt gagatctcag cgaaattcgg ccaaaacatc gaagaattgc
    13141 tggaaatgat cctgttagtc gcagaagtag aagatttaaa agcagatcca acacaacgcg
    13201 caatcggaac agtgatcgaa gcacgtcttg ataaaggtaa aggtcctgtg tcaaccttac
    13261 ttgtccaaga aggaacactt cacgttggtg acccaatcgt tgtcggtaat acttacggac
    13321 gtgtccgtgt aatgacaaac gatcttggcc gtcgcgataa agaagcagga cctgcaacgc
    13381 cagtagaaat cactggcttg aatgatgtac ctcaagcagg tgaccgtttc gtagtcttcg
    13441 aagacgaaaa gacagcacgt gcagcgggtg aagaacgcgc aaaacgtgca ttattggaaa
    13501 accgtgctgc cacaagccgt gtgactttgg acaacttatt tgaaagcctg aaagaaggcg
    13561 aattgaaaga agttaacgta atcatcaaag cagatgtaca aggttcagca gaagcactgg
    13621 ctgcatcatt gaagaagatt gatgtggaag gtgtccgtgt aaaaatcgtc cattcagctg
    13681 tcggagcaat caatgaaagt gatgtaacct tagcagcagc cagcaacgct attatcatcg
    13741 gtttcaacgt tcgtcctaca ccgcaggcaa aagtgcaagc tgatactgaa gaagtcgata
    13801 ttcgtcttca ccgtatcatc tataaagcca tcgaagaaat cgaaactgcg atgaaaggga
    13861 tgctagaccc tgaatttgaa gaaaaaatca ccggtcaaat gatcgttcgt gaaaccttca
    13921 aagtttctaa agttggtacg attgcaggtg cctatgtaac agaaggatat attcgtagag
    13981 atagcggtgt ccgtgtaatc cgtgacggaa tcgtaatcta tgaaggccaa cttgcaagtc
    14041 tgaaacgatt caaagatgac gtgaaagaag tcaaaatggg ttatgaatgt ggtgcgatga
    14101 ttgaaaaatt caatgacatc aaagtagatg acgtaattga aggatttatc atggaagaag
    14161 tcaaaaatga ctaaaaactg ataaaaggag gccaatcatt atggctaact accgtgatcg
    14221 ccgagtcggt caagaaatct taaaagaagt caatgatatt ttgcgcaaaa aggtccgtga
    14281 ccctcgtgtg gagaacgtaa cgatcacaga tgtgaaagtt acgggggact tacagcaagc
    14341 aacgatctat tacagcattt tatctgactt agcatcagat aagaaaaaag cacaagatgg
    14401 attgaataaa gcatcaggat tgattcgtcg agagttaggg cacaatctaa gtatttataa
    14461 gacacctgaa ttgttctttg aacttgatga atcagttgtc tatgggaacc atatcgatga
    14521 attgcttcgc gacttgaaca aagactaatc aaaaaatccc gcaaatcctg tgaaatcagg
    14581 ggttgcggga tttttttgtt ttcattaaga cgtattacct aagagttcca tgactttagt
    14641 ctggtagcgt gtcagctaac caaaaaagag aaaaagaaaa gtaaaaaatt cgactttctt
    14701 ttttctatag tatataaatt tttgcatata gataaagatc aagataccga taacggaagc
    14761 tagattgtaa tctattttag tgacagtcag gtatagccaa gtaactagaa gatcagtgga
    14821 acctgcacca taaaagtttg agtttgtcgg tccgttgaca gttaaaagaa aaatcacgtt
    14881 gaagttattg atatttccga ttacgccgct tcgatttggt cacgatccaa attttggata
    14941 atccctgtag aaattagcat agtagctggg ataccaattc acatattgac aaagataaca
    15001 gagactttta cataaaacgg atcaatcagg aaaggaattg ctcgatccag ccccagtttt
    15061 ccaacaatgt gttaactggc ctagcgtcat tcagcaaatt atgcatgatg aggagcgaaa
    15121 tgaactgcgg aacagccatt gtgataacga caagtttttt ggatcaatgt aaaaaacgtc
    15181 agcgaaagga aaaatttcgt ggttttttat acctgttcat ttggctagat ataaaatcca
    15241 aacaaatgaa cagaagtgct tattaggttt caattgggag tatctgcgct agaataagag
    15301 taataatgaa aaggagtgtt atgtatggat tattctagta aagaatactt tgataaaatg
    15361 acggcttggt ggcgtgctgc taattatctt tctgttgggc aactttatct taaagataac
    15421 cccttactga gacgtacact gaaaccagaa gatgtcaaaa aacatccaat cggtcattgg
    15481 ggaacgattc ctggccaaaa ttttatttat gtccatttga accgagtcat caataaatat
    15541 gatttgaaca tgttttatat cgaagggcca ggacacggcg gacaagtcat ggtatcgaat
    15601 gcttatttag atggaagtta cacagagatc tatccagaag taacggaaga tgagacagga
    15661 atgcaaaaat tattcaagcg attttcattt cctggaggca ttgcttctca tgcagcacca
    15721 gaaacaccag gttctatcca tgaaggcgga gagcttggat actctctttc acatgcagtc
    15781 ggagctgtat tagacaatcc tgaagtaatc tctgctgtcg tgattggtga tggtgaagca
    15841 gaaaccggtc ctttagctgg aagctggttc tcaaatgtct ttatcaatcc agtgatagat
    15901 ggagctgttt taccgatcct tcatttaaat ggtgcaaaaa tagccaaccc aacgatcttg
    15961 gcacgtaaat cagatggaga attagcaaat tactttaacg gacttggctg ggagcctttc
    16021 tttattgaag gaaatgatcc agaaaaatta aatcctgtta tggctgaaaa aatggatcaa
    16081 gcaatcgaga aaatcaaatc catccaaaaa gaagctcgcc tgaagacagc aacagatgtc
    16141 gtgatgccaa aatggccagt attgatcgta cgcacaccaa aaggatggac cggtccaaaa
    16201 gaatgggacg gggaaccaat tgaaggaacc ttccgagcac accaagtacc gattcctgta
    16261 gatcaagaac atatggatca tgcagatgct ttactccgtt ggctgaaatc ctatgaacca
    16321 gaaaaattat ttgatgcaca aggacgtatt ttagaagaaa tcagagaaat tgcgcctact
    16381 ggtgaccaaa gaatggcaaa aaatcctatc accaatggtg gaatcgatcc aagaccgctt
    16441 attatgccag attggaaaaa atatactttg caatttgaaa aaccaggctc cgttaaagca
    16501 gaagatatga ccgaacttgg taaattcgtt cgcgagatca ttgagaaaaa tccagaaaat
    16561 ttccgtatat ttggaccaga tgaaacaaaa tcaaaccgat tgaatcaagt tttcaaaacg
    16621 acaaatcgcc aatggatgga aaaaatcgaa ccagaaaatg atgaatggct ttcgccatct
    16681 ggtcgcgtca tcgacagtca attatcggaa caccaagatg aagggttctt agaagggtac
    16741 gttctgactg gacggcatgg tttttttgca agttacgaat cattcttgcg agtggttgac
    16801 tctatgttga ctcagcactt caaatggata cggaaatcac atgacctttc ttggcgaaaa
    16861 gattatcctt cgctgaatct cattgcaagc tcaacagtct tccagcaaga tcacaatgga
    16921 tatagccacc aagatccagg aatattgaca catttggcag aaaagaaggc ggagttcatc
    16981 cgtgaatatc tgcctgcgga tgcaaataca ttgctcgcag tcatggataa agcattccgc
    17041 tcgtcagaaa aaatcaattt gatcatctct tccaaacatc cgcgtgctca attttactca
    17101 gcagaagaag cagccgtctt agtgaatgaa ggcttgaaaa tcattgattg ggcttcgaca
    17161 gcaaaagagg aggaacctga attggtcatt gctgctgcag gaacggaatc taacttagaa
    17221 gcattagcag ctgtaacttt gctgctggaa gaattcccaa aactaaaaat tcggttcatc
    17281 aatgtcgtag atttattgaa attacgtcat ccaagtcaag atccgcgtgg gttgagcgat
    17341 gaagaatttg atcaatattt tacaaaagac aaaccgatct tatttgcttt ccacggttat
    17401 gaaacattgg tccgcacgat tttctttgat cgtcataacc atcatttaat gattcatggc
    17461 tacaaagaaa atggagatat cacgacacca tttgacatgc gtgtagtaaa tgaacttgat
    17521 cgttatcatt tggcaaaaga cgcagcttta aaaatcaaag gaagtcaagc agaagatttt
    17581 gcggaaaaaa tggatcagaa attacaagaa catcaaaact atattcgtga aaatggtatt
    17641 gatctgccag aagtattgga ttggaaatgg aaaaatcttg accaataaaa tgataaaatc
    17701 gatcccgcaa aacagaattt tttacattgt attatttgtc aaattaag
//
LOCUS       DAEGIQ010000052        17746 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000052 DAEGIQ010000000
VERSION     DAEGIQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.052"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1031)
                     /locus_tag="KMT45_001603"
     CDS             complement(<1..1031)
                     /locus_tag="KMT45_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7005069.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="KMT45_001604"
     CDS             complement(1113..1802)
                     /locus_tag="KMT45_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBE7005070.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="KMT45_001605"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="KMT45_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBE7005071.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="KMT45_001606"
     CDS             complement(2329..2832)
                     /locus_tag="KMT45_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBE7005072.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="KMT45_001607"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="KMT45_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBE7005073.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="KMT45_001608"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="KMT45_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBE7005074.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="KMT45_001609"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="KMT45_001609"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBE7005075.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="KMT45_001610"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="KMT45_001610"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBE7005076.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="KMT45_001611"
     CDS             complement(6444..7463)
                     /locus_tag="KMT45_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBE7005077.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="KMT45_001612"
     CDS             complement(7636..8541)
                     /locus_tag="KMT45_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBE7005078.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="KMT45_001613"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="KMT45_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBE7005079.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="KMT45_001614"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="KMT45_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBE7005080.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="KMT45_001615"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="KMT45_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBE7005081.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="KMT45_001616"
     CDS             9754..10398
                     /locus_tag="KMT45_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBE7005082.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="KMT45_001617"
     CDS             complement(10454..10957)
                     /locus_tag="KMT45_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBE7005083.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="KMT45_001618"
     CDS             11554..12519
                     /locus_tag="KMT45_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBE7005084.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="KMT45_001619"
     CDS             complement(12618..13790)
                     /locus_tag="KMT45_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7005085.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="KMT45_001620"
     CDS             complement(13777..14922)
                     /locus_tag="KMT45_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7005086.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="KMT45_001621"
     CDS             complement(14936..15868)
                     /locus_tag="KMT45_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005087.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="KMT45_001622"
     CDS             15985..17064
                     /locus_tag="KMT45_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBE7005088.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17690
                     /locus_tag="KMT45_001623"
     CDS             17061..17690
                     /locus_tag="KMT45_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7005089.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
BASE COUNT         5332 a         3698 c         3192 g         5524 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    17221 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    17281 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    17341 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    17401 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    17461 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    17521 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    17581 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    17641 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    17701 acacaaaaaa acctgaatga tgctccttcg catcattcag gttttt
//
LOCUS       DAEGIQ010000053        17732 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000053 DAEGIQ010000000
VERSION     DAEGIQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.053"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            86..916
                     /locus_tag="KMT45_001624"
     CDS             86..916
                     /locus_tag="KMT45_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBE7005090.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1160..1478)
                     /locus_tag="KMT45_001625"
                     /pseudo
     CDS             complement(1160..1478)
                     /locus_tag="KMT45_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1600..1794)
                     /locus_tag="KMT45_001626"
     CDS             complement(1600..1794)
                     /locus_tag="KMT45_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005091.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2299..2484)
                     /locus_tag="KMT45_001627"
     CDS             complement(2299..2484)
                     /locus_tag="KMT45_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005092.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3334..3675)
                     /locus_tag="KMT45_001628"
     CDS             complement(3334..3675)
                     /locus_tag="KMT45_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBE7005093.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3675..3905)
                     /locus_tag="KMT45_001629"
     CDS             complement(3675..3905)
                     /locus_tag="KMT45_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE7005094.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4192..4530
                     /locus_tag="KMT45_001630"
     CDS             4192..4530
                     /locus_tag="KMT45_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005095.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4735..4974)
                     /locus_tag="KMT45_001631"
     CDS             complement(4735..4974)
                     /locus_tag="KMT45_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBE7005096.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(4950..5297)
                     /locus_tag="KMT45_001632"
     CDS             complement(4950..5297)
                     /locus_tag="KMT45_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBE7005097.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(6029..6610)
                     /locus_tag="KMT45_001633"
     CDS             complement(6029..6610)
                     /locus_tag="KMT45_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE7005098.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6793..7419)
                     /locus_tag="KMT45_001634"
     CDS             complement(6793..7419)
                     /locus_tag="KMT45_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBE7005099.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7441..8772)
                     /locus_tag="KMT45_001635"
     CDS             complement(7441..8772)
                     /locus_tag="KMT45_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBE7005100.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8927..9328)
                     /locus_tag="KMT45_001636"
     CDS             complement(8927..9328)
                     /locus_tag="KMT45_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBE7005101.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9414..9620)
                     /locus_tag="KMT45_001637"
     CDS             complement(9414..9620)
                     /locus_tag="KMT45_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005102.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9715..11217)
                     /locus_tag="KMT45_001638"
     CDS             complement(9715..11217)
                     /locus_tag="KMT45_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBE7005103.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11235..11690)
                     /gene="argR"
                     /locus_tag="KMT45_001639"
     CDS             complement(11235..11690)
                     /gene="argR"
                     /locus_tag="KMT45_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBE7005104.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11788..12405)
                     /locus_tag="KMT45_001640"
     CDS             complement(11788..12405)
                     /locus_tag="KMT45_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBE7005105.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12626..13999
                     /locus_tag="KMT45_001641"
     CDS             12626..13999
                     /locus_tag="KMT45_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7005106.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14074..15186
                     /locus_tag="KMT45_001642"
     CDS             14074..15186
                     /locus_tag="KMT45_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBE7005107.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15258..16895)
                     /locus_tag="KMT45_001643"
     CDS             complement(15258..16895)
                     /locus_tag="KMT45_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBE7005108.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17063..17671)
                     /locus_tag="KMT45_001644"
     CDS             complement(17063..17671)
                     /locus_tag="KMT45_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBE7005109.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5001 a         3627 c         3111 g         5993 t
ORIGIN      
        1 tataattcgc gagcgctttc tttgaatttt ttaatctttc gtagcagaat gagaatgtat
       61 taaaaataga ttaggaggaa ttttgatgtt tagacaccag aaagaattgc agtttgaagc
      121 aaaaccagat cgtccgaatc cattaatcgc gaaatatctg caggagctga tcggcggcca
      181 atatggcgag atgagtgtag ctatgcagta cctgttccaa ggctggagct gccgaggaga
      241 agaaaaatat aaagatatgc tgatggacat tgcaacagaa gaaatcggcc acgtggaaat
      301 gttggcgaca atggttgctc gtttattaga gggtgcgcct gttgaagatc aagaagcagc
      361 gatgcaaaaa gatccaacaa tcggtgccat tatctctgga atgaatccac agcacgcgat
      421 cgtagctggc ttaggacctc gtcctgctga cagcgtgggc aatccatgga gcggtgccta
      481 tatcattgcg agtggcaact tgttagcaga ctttagagct aatttaaatg ccgaatcaca
      541 aggaagactt caagtagcac gtatttacga aatgatcgat gatcgtggcg taaaagatat
      601 gctgtctgtt ctccttgcaa gagatagcta tcatcaaaat ctatgggaat gtgcagtgaa
      661 agaattagaa gaaaaagaag gttcaatctt agtcccaagt actttcccgc gtgacaaaga
      721 acgaaaagac attgcttatg acttctataa cttctcagag ggagaccaaa gtagtgaagg
      781 ttcttgggct agtggcaaag cactggatgg cgaaggcgaa tatcaatggt tgaaagaacc
      841 taagatcgaa ggcgctgcac caaaattaga accagtaaca ccaaaaatgt atgggacacc
      901 tccattcaag aaataaatat ctacccgtag aaacacccgt tttttgttcc acacgctttg
      961 tgagcaaaac tgactaaagt cattaatcat aaccaccctt caaaagggtg gttttctcta
     1021 gagctataag ctcaaatcac cggccagcgc ctaaagacgc tggctttcat attttatgtt
     1081 caagccatat tgcctttgcc agttttgccc acctcttaaa aggtttatgt actactcctt
     1141 agcccttaaa aggatcgtct tactctcgta cactcagctt atctaacgca ctatcatgtt
     1201 tttcttgttc ttgaatgtac tttttgatgg tcgcttcatt ttgtcctact tttgtcacat
     1261 agtatccttc cacccaaaag tgtcgatacc caaatttata ttttagattt gcacaatctc
     1321 ttgatgatct cctgaatatt ggctcgatat tgattataaa ttatttttct tctatacttt
     1381 ggtgtgaata caatgtggta tttacacatc tactttgtac gggctaaact atttcccttt
     1441 attgctccac ccgcatagca agtggttttg tatttcaatc gctcggcgcg ctcaattagg
     1501 tctaaagaca ataaatctaa aaacccgctg cttcactgac agaaacaacg ggttttcaga
     1561 aaaaaatgaa atgaaaaaga tctcttacgg caatctattt tatttttttg attttttata
     1621 aatggaaaat gcttcaagaa gtccaagaaa taaagccgta taaaaacaag aacctatgaa
     1681 atctttattt attagatatt caacagaaat cccgataaca ctagccaaca atatatttat
     1741 cagaagtgta aaataaggat gctttttata gaatagaact agtgttgttt tcatagactc
     1801 tcctttcttc atatttccaa ttaatcacaa attcacggca ttactagaaa aattaaaggg
     1861 gcgcagaagt ggaaaaatac tcttcgactt tccctacaaa ccaggccatc ttgtgtttaa
     1921 taactatacc aaatctaagg tacctctaaa tctaaattcc atttgtggcg gtgacctcca
     1981 atttttaact cttctatctc ccatccagta tacataacct tgatacttcg accacttata
     2041 tatcgaacac acaaatacct atccattgca ggagtactac ctaacttagt tgctacttgc
     2101 atctaaataa cagctgaaaa gataggactt actcttagta aagtatctga actattaatt
     2161 tttaaactat catttttatc ctcaactaca tgcacatctg ttgcaacttc actttctgtc
     2221 tcatccagat aaatgattaa ggaatcggga attgcaatat cttcagctga cgctggaata
     2281 gttgagctaa attagtcatc atatctttat tataaaataa ccaacagcaa aaaatacaat
     2341 gaaaaacaaa cttggaacta atgtatcatt aataaatgtt gaaaaattca gcaactcatt
     2401 actgtatgat tttagcagcc caaccaacat ataccctata aaagctaata agatggttag
     2461 gaatccttta gaatttttac tcattcatag acacttcctt aaataacttt tattatagaa
     2521 ggtattgtaa ataataatat tgacaaaaag tgactcaaaa taaaaaaaac cttacattat
     2581 actggaatga actgtattga tagtgtacct aaaagctcta ctgacataag atcgtgtaat
     2641 cggtaaagta attttattaa ttatacggga tgtaatcaat tagagaataa actaatcata
     2701 aaaggtactt ttacattcat aattgtcgct ttgagattct actttggttc actatctgac
     2761 ccaattgcac attttcgatc attacaagta ttggtgtcgt tagaacaact aaataaaaac
     2821 tagcggtaga tttttcagaa tctatgctaa tttctgtatg gatgttccag tgatggcaaa
     2881 tttctctttt cattgttttt cttgtcgcat gatatgcctt tttgtgttat tcagttaaac
     2941 tatagaaatt ctttcctaga cagatcttga tcccaaattt tacgtttttg agcatcgata
     3001 tagtttaaaa tgacattact catatctaaa gagtttagat tttttaggac taaaactgtc
     3061 agttgttctt tttcttcttt ctgttaaagt attgtcgcga cttttcgtat acgttaagat
     3121 atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa tgacttgagg aaatcacctc
     3181 aatcattcgt ttaaaatgct atatattctt tagtttaaga tacggaaatt aatgccatcg
     3241 accacactag tagaatgaac gacatttact tggccttttt ttaatttcgt tatgtttagc
     3301 atataaaaat attatagagt ttacttacaa tgcttaaaac atatactgta taatctgatc
     3361 aattttagcc atgtcttgca atggtaagtt ttctattttt ttgagttttc gttccttaaa
     3421 atcaagagat tttaattgtg taataattac ttgtcctttt atatctagct cttcaggtaa
     3481 tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt ggacatatga ccgaaaacat
     3541 tgttttacgg ttaaattcat aacgagagac gactagtcct ggtcgtcttt tttgaatttc
     3601 ttttcctacg gagggatcaa aatcaatcca tacaatatca ccttttttag gaatataaat
     3661 tctttcttct gacactataa aatttctctc ccttcaggag ttaaatcttc ccattcgtct
     3721 ttctcataat actcggcctc ttccccacca ctaaatggat cttcaatttt tggaactaac
     3781 gtaattgttc catcatcaga atacacaacg atatattctt ggttttctga aggtttcttt
     3841 ccgttatcag aaggtaaagt taccactaca gaacttcctt gtaatctaga ttttgtaaca
     3901 agcattatca tcactccctt aataaaagta tactatataa cactaagtaa cgcaaaattc
     3961 ccctttgtaa actatctaga aaattgtttt gtttaaagta tatcttattg gatataaata
     4021 gaaataaatt gtagccagtt cattttttct ttaactaaaa aaataatcca agaaacattg
     4081 aacctatgga tccaaaagct acccaaatac tatagctaac cccccgctac cttcaatttt
     4141 gcagataaaa cgctttggga gaaaatttca aagacaacag gccatcctat catgcccgat
     4201 tataaacaaa aagctggcta ttccttttgc tctgacaaat ttcaattggg aaagttcacg
     4261 aaactagcag gtgaagaagt ttcaactgtt cggatcaaag aatgcccgct tcaaatagaa
     4321 acattggttc aacgaatttc aactagacaa gactttgcta ttgtagaacg tgagatcgta
     4381 gctatttttg tagaagaagg gattttacat gatccaactc atatcaatgt ggataaatgg
     4441 caacctctta tctataaatt cagagaatat acaactgcca accagtcgtt aactttgaat
     4501 tttagattcc aagaattcaa agagtcataa tgataaacat cgttaggtat aaataaaaaa
     4561 gactcgcagc acgagtaaaa aacaaataga agctatacat tagaaaggtc gactgtgacc
     4621 ctatagatgc agtatatgca gcttatgagc atttcttctg ttctctttgt ttttgggatc
     4681 tgtgctgaag tctttttcgt tttttgccgc tctttctttg aaaaatcgat tatgttatac
     4741 cttctccatt actaattcag gcatctgctg agtaatgatt tttcgcgcaa aagaaatatc
     4801 tgttacccaa ggttcgattt gttcttcagt caagacaagc ggcattcggt catggattgg
     4861 tgcgactgtg cggttaggtt cagtagtcat tatgattgat tctgccattt tttcatcctg
     4921 atggctacga taaaaaccgc caatatacac tattttttcc ttatttgaaa acataaattt
     4981 gtctttttct tcattccact cattgggaaa acacaacgat tttgcaaaaa tggttgatga
     5041 aaggtctttt tttcttctac tgtctctgca cgggcattaa tcatcaattg tcctttttta
     5101 aatccggtaa atccccactt catgatttcc ggagtgagga tgttttcttc ctcttttctt
     5161 aacgtcacaa caagatttga cggaaaaact tctccagctg ctaattctgc tgcctgcttc
     5221 ggttgtttcg tctgggcttc tcgataataa ttctgaagga gctctgactc tagatcaaaa
     5281 aaataacgac cacacatcct aatccctccc aatctttttt tctatcatac actgcaacaa
     5341 gaagttcgca ccaacaaaac gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg
     5401 acaaactctc cagtgaatgg tattctcctg ccaactcctc ggtcacaagt cacaatattc
     5461 aacaaacatg agaagctgct ttttgaaatt gttccttgtg actcggagtg aaacggcagg
     5521 cttacgacct ctgggtaaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
     5581 tgcaaaaatg tagaaagcta ttttcacaaa atttttctta atactcgaaa ctaagcgact
     5641 ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
     5701 ccaaacgaaa aatatgagca gctattttta tttggctgtt cttattactt gatgcagggc
     5761 agctttttca caacctctcc actaaacggt gttcaatcag ctaaccttcg gtattagctc
     5821 aaaaaacggc aaaatatgag gaactatttt gccgtttgtt tctctaatac tcaggtcaaa
     5881 gcgctgattt cacaacctct aatacacggt gttccagaag tagcttcttc gaaaaataag
     5941 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
     6001 tgaccacttc tgtcacaacc tctaattatt aaagaacctt ttgcgaagtt aacaatcttt
     6061 ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc ctgtgtgact ttcaacaata
     6121 agatacttcc ctggatttca gcaatcaacg ctttggcttg acggtctgct tcttcatcgg
     6181 acagtttttc ttcagaaagt gccaggatat tttgtttcgt atacttgacc cattgattga
     6241 ctaattctag tagcaacaga cggtattctt catccactgt actcaactca ataatcaaat
     6301 tacctacagg acagccatga taagtgatct gtgtctcatg atcagcgaaa atccagtcca
     6361 acatttcttc taatgcttct tctggctgtt ttccagcatt taagatgtct tcgaacaatt
     6421 ctgtctgcca tacgtccata tgcgcatgga tcaccgctag acaaacttct ttttttgaag
     6481 aaaagtaata ataaaattga ccttttcctg tatcagaagc tgctagaata tctcggattg
     6541 acgtttcatg atagcctttt ttataaataa gatctttggt cgcttccaag atcttttcct
     6601 ttgttttcaa tgatattctc ctttttcatt tctcatagtc ctatattata cagtaaattc
     6661 atcatttttt cttcttaatc gtttaaaaaa gactgtgcca aattatctga cacagccctc
     6721 actataaaac tgttctcata tagctagatg ttcattgatt caagaactgc gaggacaaaa
     6781 gcaattccta tcttatgata gttgctctaa tacttcctct gttgtcatca cttgatctgc
     6841 aataaagcca aagttcgtca acgctgcatc cattgcttct tggccaggag caccaattgc
     6901 atctgtcgct accacaacat tgaatccact ctctaccaat tcacgcatat gggaatcgct
     6961 acacaagttt ccgttcattc caccaagaat tactgtgtcg attccatttt ttctcaattg
     7021 gagcgccaaa tcattgcttt ctggtccaaa gattttatga ggagagctga tgatcgtatt
     7081 ttcatccaaa taaggtttca attcttcgat aaaatcggct cctgaacctt cttccactga
     7141 ttcatattga ctttttctag caaacatctg ctgctcaagc atcatatgtt ctcctgcacc
     7201 gccaaattgc catttatggt catgtgggta aaaataatga ggagaaataa agagtttgta
     7261 tcctttttct tttgcttgtt ttaccatttc aatcatattt tcgatcgtat tgtatttttc
     7321 taagatatct tttgtcaaac caaagccacc gcctgctggt ttcaagaact cgacttgcgg
     7381 gtcagtgaga actactgctg tatttttatt attgaacatg gtggattctc cattcgttat
     7441 ttattttaat acatcattta gagcttcact cattgtcgga tgagaataga tttgatctct
     7501 caataacgta taaggaagtt ttccattcat agctagcgaa atcaagttga tgacttcatg
     7561 agaatcttcc gcatagatac ttgcccctag aatcaagtca gtttcagggt cgactaaaac
     7621 tttgaataca cctttaggat cttctaatac ttgtgcttta gggacaccgc ttgtcatata
     7681 cttgaataat ttgtaattga cgccttgtgc tttggcttgt ttttctgtta aacctacatt
     7741 ggataaagtc ggagtgataa atacactgta aggcacagct ttccggttat tcgttgttcg
     7801 tttattctcg cccttcaact gatccataat gattcgataa tcatctagag agatataagt
     7861 gaactgcaat ccgcctttga cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag
     7921 gtattcatcc acagcgattg ctccgcgatc tgttagttta acatctgtat tttctaatcc
     7981 aagtgatgct gtacttggtt ttcttccagt tgcagccaaa acttttgacg cttggattgt
     8041 tacttcttgg ccatcttctg taaaggtgac ggcagctgtt gattcttggt ctgctacttg
     8101 atctacagat acacctagat ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat
     8161 ttgcgcgata tcttcatctt cacgtggaag aaattcagaa tgggcatcaa gaacgacaac
     8221 tttagatccg tattcattga acattgatgc aaattccaag ccaatatatc cagccccgat
     8281 gatcactaat ttttccggta attgttcctg atccatcgct tgcgtagaat caatcaagta
     8341 gcgacttgta ttcaatcctt tgattggtaa aatgattggt tcagaaccag tattgatgaa
     8401 aatccgttcg ccttcaacaa tcactttttc accattattc gtcacttcga tttgatggtt
     8461 agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg
     8521 ataatttttg gcgcgaagca tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa
     8581 gtcgattcct ctttctccat tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat
     8641 acatgttccg ccatacatac gtgttgactt ttcaacgact aaaaccgatt caccttttga
     8701 agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa ccgatcacaa tattttgata
     8761 tttttccatc atttttccct cgcttctttt ttacctaccg attggtacaa aaatatgata
     8821 accgataatt gtctattctc caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa
     8881 agcggtcctt tcacgttttt agtctggttc gatttccttt attcttctaa acaatcgtta
     8941 cttctacatc gatatttcct ctagttgctt tggagtaagg gcagaattca tgcgcatctt
     9001 ctgcaatttt ttgggcttct tcttcgctga tgccatcgat atgcacttct aatactacac
     9061 cgatctgata gccgccatca tctaaattat aaagagagac acgtgctttt actgtggact
     9121 ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc cccattgaaa caagaagcat
     9181 aacctgctgc aaataattgt tctggatttg ttccttctgc atggattcct ggaggagtga
     9241 ttttcatatg catcgagcca tttggtgcct cgacttctcc attgcgcccg ccatggttga
     9301 tcattgctgt ttcgtatgct tttttcattg tactgctcct ttcgattgac catttgctac
     9361 ttggctataa ttatagtgta tgacaagtaa ggcgctttct acaaaaattc tgcttattcg
     9421 aataccagaa tgccgtcttc tactattttt ccaaaaaaca aaaaaatact actcgtgaac
     9481 aacagcgtca tatttgttcc aaaataaata gcaatctttt ctacataatc ttttatatga
     9541 ttgattttaa gatacatata taccagaaag ttgaccccat aaaccaatac tgttaaaaaa
     9601 ataaccatat tcgttaccat tttttcctcc ttttgctggt ctccctacca gcactcgatt
     9661 taaccatacc actttttttg ccgtccaagc aactagcctt tcaaaaagac tggtttattc
     9721 tgctgttaat gattcaagat cagaaaaatg tccgaataat tttgctaatt ttactaacgc
     9781 caacttgttc tttccgaatt catcattttc tatctgtaat actagtaacg gctcatgtaa
     9841 gcttaaacgg atcaaaaacc agccgtttcc gtatggctct tctagatcat atcgaatacc
     9901 ttcttcattt tccgggtcga agttcatccc tttttcttct tccaacagtc gcttcatttc
     9961 ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg aacaatttta atcggatttc
    10021 ttgtacttct acgggctgtt tcaactcttt gatttgttca tctaatgtga taccttttct
    10081 ttttaattct ggcaaaagca tcaggatctt tgcgatgaca tatgcaccat catccagaaa
    10141 atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg gctagctgtg tatctttccc
    10201 aagacggtta agttctaatg ctttattgat tacatttcga tagccggaaa tataacgaac
    10261 ttggatgcct cctttttctt caatgaatct ttgtagatga gaagaagtag gggaattcgt
    10321 cacgattctt gttcctggat gctcttttaa tacgattgta cttaatactg cgatcaaatt
    10381 atttcgattg atcactgttc cagaagcact gacaactgca gaacgatcca catctgtatc
    10441 gaaaatgatc ccaagatctg cttcatttgc taaaacagcc tgcttgatac tttccatcgc
    10501 ttctttatta tcaggatttg ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg
    10561 ggaacctgtt gaatccgcac ctaagctatt caatactttt tcagcaaaga atccaccagc
    10621 accatttccg gcatcaacaa tgattttcca accatttagt ggtttgcttt ctagtgttcc
    10681 aatccccttt ttaatttttg tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt
    10741 ttctacgctt ccagtttgtt cgctttttat tttttgctgc tgtgatagga taaaagcaat
    10801 atcttctttt tctgctcctc cttctgaggt aaagaatttt attccattat aataataggg
    10861 aagatggcta gcagtcagca taaccgcagc atcgcacgag aactgttcaa attgagtgga
    10921 cataaacata gcaggcgtag tagctaattc gaaatcgatg acatgtatcc cttgttcttt
    10981 cagactttgg atcaacgcct ctttcagtgc tggtccgctt atcctactgt catgaccaat
    11041 cccaatcgtc cattttttat ttggatcggt tgatttcttc tttttcttta tccagtttgc
    11101 tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag tttacttcat atccttcaac
    11161 tggtaatgcg attcctcgaa tatccgatcc attttgcagc gctttcatat ctttcatata
    11221 aaagtcctcc tcatttattc cctcattttt tccagctttt ctgcaaatcg ctgtgcttct
    11281 tcttctgagc ggcaaataat aatgaatgta tctacaccgg ccatcgttcc gacaacttca
    11341 gggtaagcca cttcatctag ccaattcgtt accacatcgg caccatttcg ttcagtcaat
    11401 acgataacca tgaactgaac agactggata cgcaaaacct cgcgtctaat tgctgtacgc
    11461 aaacgttctt catttaaaga aacagatggc tggttaaata atgtatagcg tactattttg
    11521 ttttcatttt gactttttac taacttcatt tctcgaatgt ctcgagaaat cgttgcttga
    11581 gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct cttcctgtgt cccaataggt
    11641 tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt tctttttcat attagacacc
    11701 tctcaatgtt gattatagca aaagataagt aatatttctt catattttgg tattttttac
    11761 ttttattttt tgttttcatt caggttgcta acctttttct attttaacac gcaaaatcgt
    11821 cttcattttt tcaggtgctg attttcttgg atcgctgatg taaatctctt tgtgttcttt
    11881 agaagctctt tcatagcctt cttttgcgat gaatgcttct aatttctcaa acgttgctgg
    11941 ttcttcggca tagggcccga cgtgtaagat ttgggcacaa agaccttctt ccatcttttt
    12001 gaatactact tgttctttca agtcttccgg aagtttgcta gaacgctgaa tggcttcttg
    12061 tgctatcgct tctgtaacaa agtcaggctg tcggatcatc aattgataag aaaaatgttc
    12121 ttttttcatc actggttcat gcaaatactt ttcttgcagt ccccattgtc cttccagtgg
    12181 atagacagta tacatctgat aatcaggaat cagccaattt tttttaggcg acatgcgtat
    12241 tgcatagctg actgcatata gacaggctac tcttcgttta aagtcttcct gattcgggtc
    12301 gccgatccct ctgatgctaa agaatgattt tttaggaacc tctagtattt gcgggctttt
    12361 tttcatacta tagctcttgt ctttacgcca ttcaattttt tccatttttc ttcctccgcg
    12421 aaattccatt tagttattta ttatcatacc tctttttgag tgacttggct aatcgtgttg
    12481 acactatttt tcagtttgtg ctagaataag tgaagtaaat aaatcgtaca gataacacgt
    12541 ttcggcgtgt cgagagagag gcggtttcta tttttataat agaaatcact ctcttttttt
    12601 acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc
    12661 agtaattgca acagggatga tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt
    12721 aactttccca actcttatca gacagtttga tttaacgact gcacaagtcc aatgggtaac
    12781 aacgatttat cttttgatga tttcgatcat tgttccgctg tctacttatt tgaatcgcaa
    12841 ttttagtctc aaaaaactct ttttagcttc aaatctgctt ttcttggcag gtataatgat
    12901 cgatttcttc tctcctacct tcatggtcct attgtttggt cgattgctcc aaggtgcagc
    12961 gacaggtatc gctcttccgt tgatgttcca tattatcttg acatttgccc ccctcaatcg
    13021 acgaggcgcg atgatgggtg tcggtacttt gacaacggcc attgctccag caattggccc
    13081 cacttatgga ggcattatga cttcacattt tacatggaat catatcttcc tgtttcttgt
    13141 gcccgttttg tttctttctt tgattatcgg ctggtttgct attcccgaga tggcagtaca
    13201 aaaaagtggt tatttggatt ggataagcgt tttaggaatt tgtcttttgt ttagtggaag
    13261 tctaacattt ttgagtacgt tgggaacagt ttccagctgg attgctttaa tcatcggcgg
    13321 tattggctgg ttcctttttt actatcgaag taaaaaagtg gagcatccac tgatccgact
    13381 gtctattctt tcaaataaaa cattccgctt gtttttgatt ggcttcttag tttatcagtt
    13441 tttattactt ggtgtatctt ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag
    13501 tccttttgtc gctggattag cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc
    13561 tttttccggg aaaatgctgg atcaatatgg tccgaagaag ccaatttttg ctgggctgat
    13621 tctttctttg attggttggc tagcattatt cctactcata ggacacgctt cagtgggtgt
    13681 attgattgct tgtcatgtgt tttatatgat cggtatcgga ttatcttaca gcaatatgat
    13741 gaccaccgga atgaatgcac tgaatgaaga actacaagga gacgggaacg caatgttcaa
    13801 taccttgcag cagttttctg gcgctgtggc tacttcatta gtagcggtca ttatcaatta
    13861 cgtccagcat catacttccc ataactatga agtgtctact acattaggat caaaagcagc
    13921 attaggcgtc ttgttgctgt tgcttctcgt aagctttgct cgattcgcct attatctgtt
    13981 ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt gcatctgccg tttttttgtg
    14041 tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa aaattgccat tatcggtggc
    14101 gggatcattg gtatgaccct agccagttac ttagatacat caaaattcaa tgttgcttta
    14161 tttgatgagg ggacaggaca ggcaactaaa gctagcgctg ggattatctc cccttggttg
    14221 tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag acggcgctgc ttttttccct
    14281 aaacttataa aagatttcca acttgatcaa acgatttacg cacaatcagg gacacttttg
    14341 cttcgacctg aaaaagattt agctgaattg gctgttcttg ccgagaaacg aaaagaagaa
    14401 gcgccagaaa ttggaaacat cgaatggctt tcacctgaac agacatctca agcacttccg
    14461 ttattgaaac cagcagtttc actgcgtatc agtggtggtg gtcgtctgga tggaaaagca
    14521 tatttagaac atcttggtca gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa
    14581 gtcactttgc ataagaagag cgattcttgg ctcatcaagg gaccgtttgg agaatacaca
    14641 gctgatcgtg taattttgac acctggcccg gcacttaagc ctttattaca agaattaggc
    14701 tatgctgcag atatacgtcc gcaaaaaggg cagctcttag catttcagac atcttataag
    14761 atgagtcaag actggcctgt tgctatgctt gacggagaag ctgatttcat tcctttcatg
    14821 gatggaaaga tccttttagg tgctacccat gaaaacgatc aaggctggga tttggctcct
    14881 actagcgaag cttataaggg attgactaag agtacagaac agttcttgac tgatccagat
    14941 cagataaaaa actggccata cgagttccgt gtaggtaccc gtgcttatac gtcagatttt
    15001 tccccatttt tcggcgtact gccagatgat ccaagcttac tggctgctag cggtctaggc
    15061 tcttctggct tgaccacagg tccttatatc ggctatctat tagccaactt cttaaatact
    15121 ggtcattggc ctacggaaga ttatcaaaag gacatctcca cctatattac gaaaaatcca
    15181 tcatagaaaa acagcttttt caagtaagga tcccgagcga tattgcgcat ggattacttc
    15241 ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat tctccaaact ttcttttgct
    15301 tcatctggca agagatcttt tcctatctga gcttcaatga atcgtaaacg aatatcaagt
    15361 cgttgtttca ccagattttt ataggacgta tcctcaaaag aaacaggaaa atctggtata
    15421 ggtaaatagt ggatgtcttc aaatggacca aacgacttga attctgccgt ttgttgcacg
    15481 attctttcaa cgattgacaa tgtcgtttca ggtttgattt ttttatcatc gaaatatcca
    15541 gtattcagga catagcaggc tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag
    15601 tcttccctaa gaggatagac acggaaagga ttagcaaatg gttcaatgac tagctcatct
    15661 agctgtcttt ttgtagtgat attttccgcg tctgatcgtt ttgttgctaa tgttgcacca
    15721 aaagacgctg ctaaaactgg gtcattgatc ttgataattg gcggaagcga atcatctttc
    15781 atgatccaaa aaacagcatc gatcttttcg ctaagatgat cgactcggtc gggtgtcgca
    15841 taacgtgatt tgactgtccg cccatttccg tttcgtatat ctcctgttac taggactttt
    15901 tctcctttac ttgtacatgt cacgcccaca ttctgacaag taaggaaaga agaaatggcc
    15961 ggatcatcag acggatagtc ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca
    16021 aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca agatcgaaac agagtgacct
    16081 tccgtatgtt ttgctaaggt aatcgttgat tttccagaac cggacaaccc aaatacagcc
    16141 atcgtatagg tctggtcatt actagcatat tgtttcatcc caccgtggca ggcaataaag
    16201 ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag
    16261 taacgtaatc ctagcacagc cgctgtatta tggagagcat caaacaaaat gagtccattt
    16321 ggaaatgttt catgtgtcca ttcgggatca caaaagatat aaagatctcc ctcatcaaag
    16381 gaaacagact tttgataagt ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa
    16441 taagaataca aattgttctc aaatccttct ggaatcagca aatgactttg cacagaaaat
    16501 tcttcagata ggccaacgat tacttttgta tggtaaaact ggcgcttgcg actttgatac
    16561 acagcttctc ttaaaatttt ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc
    16621 cgccgagcac ttgctgttct tcccacaatg cttccatcat tgaatactag tgtcctaaca
    16681 tcagctggta aatctagttc ttcagcatgt gagattgtta aatctgtgat gatcgttcct
    16741 ggactccctt ttgctagttg ataagctgtc ttcctatcaa aaacttcatg aacattatta
    16801 ccgtaaaatg tcgtttctac aatcgatcga atactggata acagtggatt tccacttgtg
    16861 atttcgttta attcgagact ctcttttgta ctcatggctc ctcctcattc cctaaacaaa
    16921 aaaacgtttt ctttattata cattattcga gtttttattt gtactgctgc gctgtgggaa
    16981 gcaaacgagt tatttttcac acaaaaaaac cgagaacttg aataaacaag tttctcggtt
    17041 tcatcatttt ccttttatcg gcttagtacc agccgtttgc taaccagaat gttttagcag
    17101 catcccatga accataacga gatgttacat attgttctgc tacacgttct tgattggcag
    17161 ctgaatagtc gccgtttaag tatgaagcat ctaattgata acggccgatg taacgtccgt
    17221 tagtagctgt gtatgaaccg cctgattctt tttgtgcgat ccattctttt gcagagcttg
    17281 aagttggcgc tgttgcagca gctggggcag caggagctac ttcaactgtt tcaacaggtg
    17341 caacgtatgt atgttcttgc gttgctgttt cagttgtaga tgctgtattt tctgttgttt
    17401 tgttttctgt tgttgctgaa gaatcatttg aagttgggat tgttaattgt tcaccaacat
    17461 aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat tgcatcgatc attgagttgt
    17521 ctcctgcaaa tttttgtgag atcttagata atgaatcacc tgattttact gtataaactt
    17581 catcagcgtg agcatttgta gttcctacaa acattgcagt agcaccagca gcaaaagttg
    17641 tacctaaaac aattttctta aatgaattca tattaaactc ctttttttct ccgtttttta
    17701 ttctttcgac gctattaact ttatcattta ga
//
LOCUS       DAEGIQ010000054        17575 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000054 DAEGIQ010000000
VERSION     DAEGIQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.054"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..369)
                     /locus_tag="KMT45_001645"
     CDS             complement(<1..369)
                     /locus_tag="KMT45_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBE7005110.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIF"
     gene            763..2055
                     /locus_tag="KMT45_001646"
     CDS             763..2055
                     /locus_tag="KMT45_001646"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBE7005111.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            2070..3839
                     /gene="aspS"
                     /locus_tag="KMT45_001647"
     CDS             2070..3839
                     /gene="aspS"
                     /locus_tag="KMT45_001647"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBE7005112.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            3948..4823
                     /locus_tag="KMT45_001648"
     CDS             3948..4823
                     /locus_tag="KMT45_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBE7005113.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            5089..6972
                     /locus_tag="KMT45_001649"
     CDS             5089..6972
                     /locus_tag="KMT45_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBE7005114.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(7047..7331)
                     /locus_tag="KMT45_001650"
     CDS             complement(7047..7331)
                     /locus_tag="KMT45_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBE7005115.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(7321..8055)
                     /locus_tag="KMT45_001651"
     CDS             complement(7321..8055)
                     /locus_tag="KMT45_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBE7005116.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            8184..8807
                     /locus_tag="KMT45_001652"
     CDS             8184..8807
                     /locus_tag="KMT45_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBE7005117.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            8999..10120
                     /locus_tag="KMT45_001653"
     CDS             8999..10120
                     /locus_tag="KMT45_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBE7005118.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            10127..13255
                     /locus_tag="KMT45_001654"
     CDS             10127..13255
                     /locus_tag="KMT45_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBE7005119.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            13274..16468
                     /locus_tag="KMT45_001655"
     CDS             13274..16468
                     /locus_tag="KMT45_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBE7005120.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(16614..17321)
                     /locus_tag="KMT45_001656"
     CDS             complement(16614..17321)
                     /locus_tag="KMT45_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBE7005121.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         5891 a         3108 c         3739 g         4837 t
ORIGIN      
        1 aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag
       61 aaactcagga gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc
      121 gatgacgaca cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc
      181 ggaacggccg tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc
      241 tgtacgaact acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc
      301 aacggcattt gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat
      361 ttctgccata gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa
      421 ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta
      481 gtaggttata agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca
      541 agagaggatc atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga
      601 gatggacgag tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc
      661 tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg
      721 gtgatttttt tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca
      781 aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca
      841 gcacgtttat tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac
      901 tatgaagtga tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat
      961 gatttttatg acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc
     1021 gttcgttctt ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact
     1081 ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc
     1141 catcaaatcg gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg
     1201 gtgatggcac ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat
     1261 tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta
     1321 ccttttgaat cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc
     1381 gtattggaca gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta
     1441 gattatctaa gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca
     1501 ttggctgttc cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat
     1561 acgatttttg aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca
     1621 ggcggacgtt atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga
     1681 tttgcaatgg gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct
     1741 gcatttaatg agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca
     1801 ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac
     1861 cgtaaagcaa aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg
     1921 atcggtgaag atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa
     1981 gaaaaaagtt ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg
     2041 actttattcg agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag
     2101 tttccgaaca acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag
     2161 accttggggg agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct
     2221 ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg
     2281 tgatcgaagt gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg
     2341 ccactggtga attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc
     2401 cgccatttca aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt
     2461 atttggatct gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa
     2521 aagcgattcg tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag
     2581 ggaaatctac accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac
     2641 atttctatgc gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat
     2701 tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc
     2761 agcctgaatt tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa
     2821 cttacacaga gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat
     2881 tgccatttcc tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg
     2941 atacacgttt tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca
     3001 aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg
     3061 ctgcagatcg ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg
     3121 gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta
     3181 aattcatggg agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact
     3241 tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga
     3301 cacgtttagg aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa
     3361 ctgattggcc acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc
     3421 cattcacgat gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct
     3481 atgcagaagc ttatgatatc gtcttgaacg gttatgaact aggcggaggt tctatccgta
     3541 ttcatactcg tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa
     3601 tggaagaaca atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg
     3661 gtattgcatt aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg
     3721 aagtcattgc ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta
     3781 tggtttcgcc tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc
     3841 cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg
     3901 aaaaacactt tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat
     3961 tcgaaaattg ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt
     4021 tgatcgcatc agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta
     4081 ttactggtct agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac
     4141 cggtatcaac aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa
     4201 tcgggaagaa gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc
     4261 atattttacc ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg
     4321 ctgtgatggg actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag
     4381 atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct
     4441 acttcaatgc attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt
     4501 acagtacgtt gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa
     4561 agaagatgca ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa
     4621 actgtatgcg tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata
     4681 agcagacagt attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa
     4741 tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc
     4801 gtttctatga ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag
     4861 acagacgttc gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg
     4921 cttttttatt tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat
     4981 gggatgatag aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat
     5041 acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa
     5101 aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg
     5161 gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt
     5221 ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa
     5281 caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg
     5341 gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg
     5401 ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa
     5461 atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc
     5521 tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg
     5581 atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc
     5641 tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac
     5701 ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca
     5761 agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga
     5821 ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc
     5881 atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca
     5941 gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc
     6001 aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag
     6061 gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta
     6121 atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt
     6181 cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca
     6241 tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg
     6301 aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa
     6361 ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc
     6421 cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg
     6481 gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa
     6541 gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa
     6601 ttaaaggaac aagatttgtt gaaagacact gttctgatcg gactaggaac gaatggctca
     6661 ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg
     6721 gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag
     6781 atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat
     6841 agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt
     6901 cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac
     6961 gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt
     7021 ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt
     7081 gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt
     7141 gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt
     7201 tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga
     7261 tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt
     7321 tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt
     7381 gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa
     7441 caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat
     7501 tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt
     7561 aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt
     7621 atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag
     7681 ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga
     7741 taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc
     7801 gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc
     7861 ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat
     7921 cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga
     7981 aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc
     8041 gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat
     8101 ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc
     8161 ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac
     8221 tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg
     8281 agaattacat actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag
     8341 ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc
     8401 tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa
     8461 gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc
     8521 ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt
     8581 ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca
     8641 agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa
     8701 cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg
     8761 acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta
     8821 tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg
     8881 agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt
     8941 gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat
     9001 gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat
     9061 tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga
     9121 ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga
     9181 attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat
     9241 atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc
     9301 gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt
     9361 acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga
     9421 caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt
     9481 tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc
     9541 tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac
     9601 accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa
     9661 tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg
     9721 cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct
     9781 gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca
     9841 gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa
     9901 tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg
     9961 tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt
    10021 agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg
    10081 gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa
    10141 attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac
    10201 agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga
    10261 tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat
    10321 gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca
    10381 gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa
    10441 cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga
    10501 aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct
    10561 cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt
    10621 ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt
    10681 ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca
    10741 tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc
    10801 agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa
    10861 acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga
    10921 aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat
    10981 aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat
    11041 tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa
    11101 agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac
    11161 tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa
    11221 aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc
    11281 cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt
    11341 gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca
    11401 agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa
    11461 atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct
    11521 cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct
    11581 attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat
    11641 tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga
    11701 acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga
    11761 acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag
    11821 tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc
    11881 aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt
    11941 tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga
    12001 tagacgaaaa agcaagatgg ccaatgcaag aatagagctg gaagaaataa gacaacaagc
    12061 agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca
    12121 gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag
    12181 taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca
    12241 actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac
    12301 acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca
    12361 agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg
    12421 tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca
    12481 tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga
    12541 gacattccca gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga
    12601 aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta
    12661 cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt
    12721 acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta
    12781 tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt
    12841 aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac
    12901 cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct
    12961 gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat
    13021 ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc
    13081 attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg
    13141 aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat
    13201 aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg
    13261 gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata
    13321 cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc
    13381 agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga
    13441 ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga
    13501 actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga
    13561 accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac
    13621 acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga
    13681 gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt
    13741 ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac
    13801 agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt
    13861 tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt
    13921 attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc
    13981 agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca
    14041 gataaatgaa caaaacgatc aaggaataca attttccgat caaaacaaac ctttatcatt
    14101 caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt
    14161 ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca
    14221 acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt
    14281 gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat
    14341 cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt
    14401 tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg
    14461 cgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt
    14521 gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca
    14581 gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt
    14641 ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg
    14701 agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc
    14761 atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct
    14821 gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt
    14881 tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg
    14941 tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt
    15001 ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca
    15061 tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca
    15121 aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc
    15181 cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga
    15241 agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca
    15301 ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa
    15361 agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt
    15421 aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc
    15481 acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc
    15541 tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt
    15601 gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc
    15661 agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt
    15721 gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa
    15781 aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga
    15841 agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg
    15901 ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt
    15961 aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt
    16021 tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta
    16081 tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg
    16141 cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga atctcacggg
    16201 agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc
    16261 tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga
    16321 aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa
    16381 agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat
    16441 tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag
    16501 aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat
    16561 tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca
    16621 aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt
    16681 ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt
    16741 tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc
    16801 atatgaaatt ctttctcagc tagctgaaaa atctcttttt ctggtttttg gaaacctgtt
    16861 gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt
    16921 ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc
    16981 ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc
    17041 tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct
    17101 tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt
    17161 acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct
    17221 gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca
    17281 tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg
    17341 ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag
    17401 ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat
    17461 atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa
    17521 tcattatggt agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcg
//
LOCUS       DAEGIQ010000055        17528 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000055 DAEGIQ010000000
VERSION     DAEGIQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.055"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            240..608
                     /locus_tag="KMT45_001657"
     CDS             240..608
                     /locus_tag="KMT45_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBE7005122.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            788..1729
                     /locus_tag="KMT45_001658"
     CDS             788..1729
                     /locus_tag="KMT45_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBE7005123.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1798..2529
                     /locus_tag="KMT45_001659"
     CDS             1798..2529
                     /locus_tag="KMT45_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBE7005124.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2673..4853
                     /locus_tag="KMT45_001660"
     CDS             2673..4853
                     /locus_tag="KMT45_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBE7005125.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4873..5355
                     /locus_tag="KMT45_001661"
     CDS             4873..5355
                     /locus_tag="KMT45_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBE7005126.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5371..6132
                     /locus_tag="KMT45_001662"
     CDS             5371..6132
                     /locus_tag="KMT45_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBE7005127.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6263..7597
                     /locus_tag="KMT45_001663"
     CDS             6263..7597
                     /locus_tag="KMT45_001663"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBE7005128.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8025..8510
                     /locus_tag="KMT45_001664"
     CDS             8025..8510
                     /locus_tag="KMT45_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBE7005129.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8503..9186
                     /locus_tag="KMT45_001665"
     CDS             8503..9186
                     /locus_tag="KMT45_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBE7005130.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9312..11957
                     /gene="polA"
                     /locus_tag="KMT45_001666"
     CDS             9312..11957
                     /gene="polA"
                     /locus_tag="KMT45_001666"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBE7005131.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPKKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12002..12838
                     /gene="mutM"
                     /locus_tag="KMT45_001667"
     CDS             12002..12838
                     /gene="mutM"
                     /locus_tag="KMT45_001667"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBE7005132.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHYELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12802..13431
                     /locus_tag="KMT45_001668"
     CDS             12802..13431
                     /locus_tag="KMT45_001668"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBE7005133.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13483..13815
                     /locus_tag="KMT45_001669"
     CDS             13483..13815
                     /locus_tag="KMT45_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBE7005134.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14127..14618
                     /gene="nrdR"
                     /locus_tag="KMT45_001670"
     CDS             14127..14618
                     /gene="nrdR"
                     /locus_tag="KMT45_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBE7005135.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14641..16014
                     /locus_tag="KMT45_001671"
     CDS             14641..16014
                     /locus_tag="KMT45_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBE7005136.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16014..16940
                     /gene="dnaI"
                     /locus_tag="KMT45_001672"
     CDS             16014..16940
                     /gene="dnaI"
                     /locus_tag="KMT45_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBE7005137.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17069..17509
                     /locus_tag="KMT45_001673"
     CDS             17069..17509
                     /locus_tag="KMT45_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE7005138.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5970 a         3068 c         3846 g         4644 t
ORIGIN      
        1 ccagaggttg tgacttgttt ttagtcacga cctctttttt tatttattcc atataaatac
       61 acattattta ttccgaaaag tgaaagcgta aataacaaaa acaaccaact tatttattat
      121 taaagaaaag cgtatttaag taggacgatt ctgttgagag ctttcgtaga ttttcccatt
      181 ttcctatggt aaactgacaa agttgagaat gttagaacag atcgtttagg aggaaacaga
      241 tgtttacgaa tgaaataaaa atcatgcttt atgtggacga tgtagaaaaa aatgcaacgt
      301 tttggagagc agttggcttt gcggaaaaag atcgtcaaga aatggatggg acgcttataa
      361 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      421 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      481 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      541 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      601 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      661 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      721 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      781 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      841 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      901 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      961 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
     1021 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
     1081 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
     1141 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
     1201 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1261 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1321 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1381 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1441 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1501 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1561 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1621 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1681 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1741 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1801 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1861 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1921 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1981 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     2041 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     2101 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     2161 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     2221 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2281 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2341 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2401 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2461 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2521 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2581 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2641 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2701 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2761 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2821 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2881 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2941 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     3001 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     3061 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     3121 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     3181 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3241 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3301 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3361 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3421 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3481 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3541 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3601 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3661 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3721 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3781 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3841 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3901 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3961 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     4021 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     4081 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     4141 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     4201 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4261 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4321 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4381 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4441 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4501 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4561 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4621 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4681 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4741 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4801 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4861 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4921 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4981 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     5041 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     5101 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     5161 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     5221 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5281 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5341 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5401 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5461 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5521 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5581 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5641 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5701 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5761 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5821 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5881 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5941 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     6001 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     6061 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     6121 tcccgtactt gaccatttta gcaagcgaat cctcctttac aaataaattt ggttgaaatt
     6181 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6241 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6301 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6361 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6421 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6481 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6541 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6601 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6661 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6721 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6781 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6841 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6901 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6961 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     7021 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     7081 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     7141 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     7201 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7261 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7321 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7381 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7441 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7501 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7561 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7621 aaaaatagaa agagggggag tggcaaaaat cttgccaatc cccctttttt gcaacttatg
     7681 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaatc aaaaacatga
     7741 gaagctgttt ttcttggctg ttcttattac ttgatgcagg gcagcttttt cacgacctca
     7801 gataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaaat gaaaaatatg
     7861 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7921 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7981 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     8041 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     8101 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     8161 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     8221 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     8281 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     8341 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8401 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8461 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8521 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8581 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8641 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8701 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8761 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8821 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8881 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8941 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     9001 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     9061 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     9121 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     9181 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     9241 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     9301 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9361 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9421 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9481 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9541 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9601 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9661 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9721 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9781 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9841 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9901 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9961 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
    10021 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
    10081 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
    10141 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
    10201 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
    10261 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
    10321 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10381 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10441 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10501 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10561 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10621 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10681 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa aaccaagaag
    10741 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10801 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10861 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10921 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10981 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    11041 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    11101 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    11161 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    11221 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    11281 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    11341 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11401 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11461 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11521 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11581 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11641 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11701 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11761 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11821 ttgtcttcga ggtaccgaaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11881 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11941 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    12001 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    12061 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    12121 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    12181 tttaattttt catctcgatc attatgaact gatctcacac ttgcgaatgg aaggaaaata
    12241 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    12301 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12361 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12421 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12481 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12541 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12601 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12661 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12721 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12781 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12841 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12901 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12961 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    13021 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    13081 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    13141 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    13201 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    13261 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    13321 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13381 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13441 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13501 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13561 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13621 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13681 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13741 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13801 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13861 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13921 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13981 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    14041 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    14101 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    14161 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    14221 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    14281 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    14341 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14401 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14461 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14521 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14581 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14641 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14701 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14761 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14821 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14881 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14941 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    15001 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    15061 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    15121 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    15181 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    15241 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    15301 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15361 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15421 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15481 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15541 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15601 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15661 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15721 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15781 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15841 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15901 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15961 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    16021 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    16081 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    16141 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    16201 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    16261 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    16321 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16381 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16441 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16501 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16561 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16621 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16681 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16741 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16801 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16861 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16921 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16981 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    17041 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    17101 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    17161 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    17221 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    17281 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    17341 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17401 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17461 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17521 ttttcaaa
//
LOCUS       DAEGIQ010000056        16734 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000056 DAEGIQ010000000
VERSION     DAEGIQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.056"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            85..1785
                     /locus_tag="KMT45_001674"
     CDS             85..1785
                     /locus_tag="KMT45_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBE7005139.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1755..1973
                     /locus_tag="KMT45_001675"
     CDS             1755..1973
                     /locus_tag="KMT45_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBE7005140.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1993..2883
                     /locus_tag="KMT45_001676"
     CDS             1993..2883
                     /locus_tag="KMT45_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBE7005141.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2901..3185
                     /locus_tag="KMT45_001677"
     CDS             2901..3185
                     /locus_tag="KMT45_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005142.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3672..4286
                     /gene="gmk"
                     /locus_tag="KMT45_001678"
     CDS             3672..4286
                     /gene="gmk"
                     /locus_tag="KMT45_001678"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBE7005143.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4291..4596
                     /locus_tag="KMT45_001679"
     CDS             4291..4596
                     /locus_tag="KMT45_001679"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBE7005144.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4849..7257
                     /gene="priA"
                     /locus_tag="KMT45_001680"
     CDS             4849..7257
                     /gene="priA"
                     /locus_tag="KMT45_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBE7005145.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7272..7763
                     /gene="def"
                     /locus_tag="KMT45_001681"
     CDS             7272..7763
                     /gene="def"
                     /locus_tag="KMT45_001681"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBE7005146.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7756..8694
                     /locus_tag="KMT45_001682"
     CDS             7756..8694
                     /locus_tag="KMT45_001682"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBE7005147.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8694..10052
                     /gene="rsmB"
                     /locus_tag="KMT45_001683"
     CDS             8694..10052
                     /gene="rsmB"
                     /locus_tag="KMT45_001683"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBE7005148.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10065..10805
                     /locus_tag="KMT45_001684"
     CDS             10065..10805
                     /locus_tag="KMT45_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBE7005149.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10802..12871
                     /gene="pknB"
                     /locus_tag="KMT45_001685"
     CDS             10802..12871
                     /gene="pknB"
                     /locus_tag="KMT45_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBE7005150.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13036..13935
                     /gene="rsgA"
                     /locus_tag="KMT45_001686"
     CDS             13036..13935
                     /gene="rsgA"
                     /locus_tag="KMT45_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBE7005151.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13948..14598
                     /locus_tag="KMT45_001687"
     CDS             13948..14598
                     /locus_tag="KMT45_001687"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBE7005152.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14599..15237
                     /locus_tag="KMT45_001688"
     CDS             14599..15237
                     /locus_tag="KMT45_001688"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBE7005153.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15392..16246
                     /gene="mreC"
                     /locus_tag="KMT45_001689"
     CDS             15392..16246
                     /gene="mreC"
                     /locus_tag="KMT45_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBE7005154.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16250..>16734
                     /gene="mreD"
                     /locus_tag="KMT45_001690"
     CDS             16250..>16734
                     /gene="mreD"
                     /locus_tag="KMT45_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBE7005155.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     P"
BASE COUNT         5610 a         3028 c         3673 g         4423 t
ORIGIN      
        1 ttcttatgga atctacaaag tcgaatacga gactcatgac ctttttatga cgtactggta
       61 tacttgaaga aaaaggagag cgcgatggag ataaaaaaag tttacttttc acagaaaatg
      121 tcgctgtatt cgcctatcca gcaagcaaaa caaccccttc cattttttca aagtcatcaa
      181 gaaagtagag acgctttttg gcgttctgca aaaaagcaat gtgcttttaa agcagtatcg
      241 aaaaaatata ccgtctattt tactattgct aaaagtagag gagatgttat ttttaataat
      301 ctgttgattg aaggtgaaac ttttgaatgg aaaaaatttt ttcggtatat ggaagcttgc
      361 gctcgttttt ttattaaaga aaactgttgt tttttatttc aatctcctct atcagaagaa
      421 tggttgcgta tttttcataa aaacggctat caaggaacca cacaattaaa taaaaagctg
      481 gtctatcata cagcacttgt tttaggtgga ggaggagcgc acggtgctta tcagattggt
      541 gtgtggcagg cgctgaagga acatgatatc aactttgaga ttatcacagg tacctcggta
      601 ggtgcgttga acggcgcatt gattttacaa ggagatatga aaaaagctgt gaatttatgg
      661 aaaaagctgt cgacaagaca agtgcttgcg ttaccagaaa tggctgcagt tgaagacctt
      721 cgtgaacgct tctatcgaga aagacgccaa atgacgaaaa cagcactaat tgaaggcggg
      781 gtttcttctg ctccattgga aaagatggtc aaagacaatt tggatttgtc cttactcaaa
      841 cataatccca aaatacgttt atatacggtg tctactaaac tgccagaact agcggaagta
      901 gtgactgata tccaacagac aaaaacagaa gaaataccag attggatact cgcctctgct
      961 tcattttatc ccgctatggc ttatcgaaag atcggaaaaa ataaatatgc agacggtggg
     1021 tatcgcaaca aaatacctat tgatattgcg attaacaaag gagcaacaga ggcatttgtc
     1081 gtagatgtgc aagggccagg cccagcgaaa aaaattcgta tgccagacat ttttatccat
     1141 tggaaatgtc aaacattgtg gacactaggc agctttctgc tttttgatag ccaaaggaac
     1201 caattaaatt tacaattagg ctatctggaa atgaaaaaac gattaggcta ttactatggg
     1261 aactggtata catttcattc tgttaaacct gcaagaacat actggcgagg atttttgtct
     1321 tacttaacaa aagaaattca gttagaatta tcttttttca aaagcataaa attttggcaa
     1381 aaactgcgta atttatataa gaacagggtc gtaccagaga catgcggatt ggcgatgcta
     1441 gaacttttag caaaaaaaca ttttttactg cccaatgaaa tctatcaagt agacatgatg
     1501 attcaaatga tctgccaaca agatataaaa agtatgccgg atgatttatt ggcacaaatt
     1561 ggtcaattaa gtactgaaga atggcgaagg tatcgaaaat accagcaaaa aatacaaaat
     1621 gagcgcacga aaactgttta ttttgattat ctgctacaag gaaaacgcaa ggatcgtttg
     1681 cagcatgaat tgcttaagca accagttgac acattactga tcctatattt atattatctc
     1741 aaggaggaac aaccatggca caagaatttt catatgaaat cgtagaagaa atcgctatat
     1801 tgtcagaaaa caacaagggg tggcgtaaag aactgaacct agtcagctgg aatggtcgcc
     1861 cgccaaaatt cgatcttcga gattgggcgc cagaccatga aaaaatgggg aaaggcctga
     1921 cattgacgaa cgaagagttt gaacaactgc aaaaagcaat agaaaatatg taatgaggaa
     1981 ggaccctgtc gaatgaaaag tatgactggt tttggcaaag ccatccgcga aacaaaagcg
     2041 tatcagttag atattgaaat aaaaagtgtc aaccagcgtt ttctagatgt tcaaatacga
     2101 agtcctaaaa tcctgaactt tgttgaaaac gattttcgtc agcttattaa acagcagtta
     2161 agccgcggtc gaatcgaagt atttgtgaat ctagcttata ttggcaaaaa cgaaaaacag
     2221 atcgtcatca actgggaatt gattgattca cttatcactc aattgacaga cggaattcaa
     2281 caacgatacg gtagtcaaaa tcaagtgtca attagtcgga tggcggaacg cttcgtgctg
     2341 gacgaaaact tcgtaacggt agaagaaaaa ccggaagaga gcatggaaga attggcacag
     2401 ttagctctag atacattgaa aagcgcactt gattctattg aggcgagtcg agaaaaagaa
     2461 ggtcaagctt tagctttagt gttgaaaaag aatactgaag aattcaaaga agtactaaaa
     2521 aaactcgata gttttgtaga aatctatgaa aaggaatttc aaataaggta tcaaaaaaag
     2581 ctggaggaat ttcttggagc gaaagtcgat gagcaacggt tgttgacgga gctagccatc
     2641 ttgttggaac gcggagatat tcacgaggaa ctggatcgtc tggcgatcca tgtacaaaaa
     2701 ctagatgaac ttatcgaagc acaatgtcct gtaggtcggg aattagattt cctgatccaa
     2761 gaaatgaatc gggaaatcaa cacgatcggt tccaaatcaa atgcaatcga aattaaaaat
     2821 caagtcgtcc agtctaagac gatcttagaa aaaatcagag aacaagtaca aaatattgaa
     2881 tgacgaaaga ggtagagata atgaaatatt atgacgtaac gttccacgaa ttaagtggaa
     2941 aaactgtggt taaacgggat atcccttctg aaaaaaacgg ctttgacgta tggaaagatg
     3001 cctgtgcaga ctataatgag aacgaactgt ttattttgat caacgacggt gcttacgtga
     3061 cgatgaatcg gaaatttatt gttcgaattg acacagaaga ggtagaagat ccgacagaaa
     3121 aagcacgttc tcgaaaagat gagatcatgg gtgtagtgaa tactttgtcc aatatgggct
     3181 tttagcaagg atgctgaaca aagaaataca ataaataaac agaattggat ttcttcgctt
     3241 agcttctttg ctctaggaat acatttaaag gttgtgacaa aattcgtgtc acaatctttt
     3301 tttattttga ataagtgatg ttcaaaaacc attttctcgg tattaagcca aatttatgca
     3361 aaaatgtgag aaactatttt cacaaaattt ttcttcatac tcgaaactaa tcggcttctt
     3421 cacgacctct tatacacggc gttcaaaagc ttgcatctgc gataataacc ccaaccaaac
     3481 gaaaaacatg agaagctgtt tatgtttggc tgttcttatt acttgatgca ggacagcttt
     3541 tttacgacct cttggtacag ggtagctttt ttaccaattt ctagctataa ttaaagataa
     3601 ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa gtacaaaaag actagttgaa
     3661 aggacgtcgt catgtcagag cgtggattat taatcgtgtt gtctggtcca tccggtgtag
     3721 gaaaaggcac agtacgaaaa gcaatttttg agagtgagga taatgatttt cagtattcga
     3781 tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga aggcgttgat tatttcttcc
     3841 gaagcaaaga agagtttgaa tcaatgatcg aagctggaga gatgctggaa tacgcagaat
     3901 atgtcgggaa ttattatgga actcctttgt catatgtcaa caagacattg gatgaaggaa
     3961 aagatgtttt cttggagatt gaagtccaag gagcaaaaca agtcaaagaa aaagtgcccg
     4021 atggtgtatt catctttttg actccgccag atttagcgga attaaaatca cggatcgtag
     4081 gccgaggaac agatgcagat gaagtaatcg aaaaacgaat gaaagttgca aaagaagaaa
     4141 tcgaaatgat ggctctttac gactatgctg ttgtaaatga tgaagtgccg ctagcagttg
     4201 aacgaatcaa aaacattatt gccagtgaac attttcgagt agatcgcgta atcggtaaat
     4261 atattaaaat gttaaaggag atgtagtctt atgatgttaa aaccttcaat tgactcacta
     4321 ttagatcgtg taaattcaaa atattctcta gtgattttag caagtaaacg cgcgcatgaa
     4381 ttggatgcaa gtgcacagcc tactctagat tcttttgatt cagtaaaaag cgtcggtcaa
     4441 gctttagaag aaatcgatgc aggaaatgta gtcaatgatc cacatcctga attaaaacgt
     4501 gaacgtttga agatggaaga agaggaacgt caagcacaaa aagagcgtga acaacacgaa
     4561 ttggaatcac gtatccgtga agagcaaaaa atgtaataaa gaaaaataga gcgaacatct
     4621 aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt cgccacttat tgtctggtag
     4681 aaaaggctgt gtctgaactt tttgacacag ttcttttttt tcttatctgc tgttaatttt
     4741 ttgttaggat ttggcagaag ataagacaaa tccatcgatc tctagtaaaa taggcaagga
     4801 actttttgtc gcttgataag aaaagacaaa aaaaggagga aacgaaccat gacattaaca
     4861 gctgaagtca ttgtggacgt acctactatg cagaccgatc agccattcac ttatttggtt
     4921 ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag tagaagtccc ttttggtaat
     4981 ggcaatcgtc atgtccaagg atttgttatg gctatccaaa aatcagaaga atcagctaat
     5041 ccatctttga aggcagttat tcgtttgcta gaccttgctc cggtggtcaa tgaagaatta
     5101 ctttcattag ctgattatat gaaaaaaatt acttacgcat tcaaaatcac ttgtctacaa
     5161 acgatgcttc cgagtgtgat gaaagcagaa tatgataaat tgatctatcc tttggcagac
     5221 acaccagaag ttcaggtatt gtttgatcag cgagaagtga tttcctggaa ggaagcagaa
     5281 gaagaaggca gcctttctca attgattcgc tggcgccaag agcaattagt agatatcaaa
     5341 tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt tggtccgttc tttgttaact
     5401 gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa atgccaagaa acaaaaagaa
     5461 cttcttttgt gtttatctga actgagccaa gaagaaccaa ttgcttattt caaaaataaa
     5521 ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg gctggctgga gtttatagaa
     5581 tcagaacgtt atcgcgaccc ttataaagac cgagtatttg accaaacgac ggcattggaa
     5641 ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca cagcaggtca gcagcaaaaa
     5701 gatcaggtct ttttgctgga gggaatcact ggcagcggga aaacagaagt atatcttcag
     5761 gcaatcgctg atgttttgtc agaaaataaa acggctatta tgttagtgcc tgaaatcgca
     5821 ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg gagaatcagt tgcggtctta
     5881 catagcggac tttcccaagg tgaaaaatat gatgaatggc gcaagattga gcgggaagaa
     5941 gcgcaagtag tagtaggcgc acgatctgct atttttgccc cgctgaaaaa tatcgggctg
     6001 atcattatcg atgaagaaca tgaatcaagt tacaaacaag atgagacacc gcgttatcat
     6061 gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt gcccaatagt tctaggaagc
     6121 gcgacgccct ctttagaatc aagagcacga gctcaaaaag gcgtttatca gttattgcag
     6181 ttaaaccacc gagccaaagc agctgcacag cttccagcta ttgagatcgt agatatgcgt
     6241 gaagagtttc agaatcatcg gacctctact ttttctgcta atttgcagga aaaaatacaa
     6301 aatcgattag acaaaaaaga acaaactgtc ctactgctca atcgtcgagg atattcgtct
     6361 tttgtgatgt gtagggactg tggatttgtt cttccatgtc ctaattgcga tatctctctt
     6421 acactgcata tggatacacg ttcaatgcgc tgccattatt gcggacatga agaacctatt
     6481 cccaatcgct gtcctaattg tggcggcaat aaaatccgct actatggaac aggtactcaa
     6541 aaagtagaag aggaactccg agaactttat ccgcaagcta ggattttgcg tatggatgta
     6601 gataccactc gaagaaaagg ggcacacgaa cagattttac aaaaattcgg tgcaaaagaa
     6661 gcagatatat tattagggac acaaatgatt gctaaaggtt tggactttcc agaagtgaca
     6721 ttagttgggg tgttgaatgc tgatacctcc ctgaatttac cggattttcg ttctagtgag
     6781 catacattcc aattgcttac ccaagtttca ggacgagcag ggcgagcgga aaaagcaggc
     6841 gaagtcgtga tccaaacatt caatcctcaa cattacgcca tagaattagc taaaaaacaa
     6901 aattatgagc agttttatca gcaggaaatg catgtacggc atcgtggagg ctatcctcct
     6961 tattacttta ctgtgaaaat aaccgccagc catcctgaag aacaagtagc ggcaaagaaa
     7021 atctttcaga ttgccaatca gctaaaagaa gtgttaagcc ctcaaagctt gctattaggg
     7081 ccaacgccca gcatgatttt gagagtgaaa aaccgatatt attatcaact aatcatcaaa
     7141 tataaacatg aaccgaatct tcctcatgtt ttggatgaaa ttttgaatgg aagccagaaa
     7201 gaacagcgac aaggtctttt tgtggcaatt gacaacgaac cattaaactt tatttaagaa
     7261 agggccaaat tatgcgctat cctatattga tccaccctaa tgataaatta aaaagaactg
     7321 ctcagccaat cgatgtaatc acggacgaga caatcgcttt attagacaat ttatatgaga
     7381 caatgattgc gaatgacgga atagggatcg ctgctcctca agtaggacaa aacaagagaa
     7441 ttgctgtgat cgaagtagat gaaggcgaga aatttgaact gattaaccca gaaatcatcg
     7501 aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag tattccgcat gtctatggaa
     7561 cggtcaagcg agcagatgaa gtgaccgtgc gttattatga tcgagacgga gaagagatag
     7621 aagtgacggc ttttggctat ttagcccgtg ccttccagca tgaaatcgat cacttagacg
     7681 gtatcttgtt cattgagaaa atgatacagc agatcccgga agaagaactt gaagaatata
     7741 tggaggaaca tcttgatgac taagctagta tttatgggca cacctgcttt ttctgtgccg
     7801 attcttgaag ggctgataga atcaggttac gagatccagg cagtggtaac acagcccgac
     7861 cgtccagttg ggcggaaacg agtaattacg ccgacaccgg taaaagaagc ggctctgaag
     7921 cacggtatcc gtgtgctgca gccagagaaa atctccggtt cgccagaaat ggaagaaatc
     7981 attgaattgg tgcctgatgt gatcgtcact gcggcatttg gccaattctt gcctgaaaag
     8041 ttattgcaag taccaaaatt aggggcaatc aatgtccatg cttctttact tcctaaatat
     8101 cggggaggtg cgcctgttca ttatgccatc atgaatgggg agaaagaaac tggtgtaacg
     8161 atcatggaaa tgatcaaaaa aatggatgca ggcggaatct atgcgcaaga aagcatacca
     8221 atcactaaac aagatgatgt agggacgatg tttgaaaagc ttagcctttt aggaagaaag
     8281 ctattgctag aaacattgcc caatattttg gatggacaaa aaccagtacc tcaagacgag
     8341 tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag aagcaatcga ttggcataaa
     8401 actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc cttggccaat tgcattcacg
     8461 acctatgagg gcatacgctg gaaattatta tcggttgaag tattggcaga aactacagac
     8521 aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc tatggattgc ttgcggtaaa
     8581 cagaccgtct tagccatcaa tgaattacag ccagctggta aaggaaaaca ggccgtacat
     8641 gatttcctta acggaagcgg tcagcaagtt cagattggtc agcaggtgaa ataatgaaaa
     8701 aaatccctaa aaaaatgaaa aaatcagtac gatatatggc attactgaca ttagagcgag
     8761 tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga gatgatcaaa caaagcgagc
     8821 taaatcaaaa agatgtgggt ttatttacag aattgatcta tggtacgatt agccatcagc
     8881 gactattgga attttacgtt gcaccattga tcgctaaagc aaaaaaagtg gatgactggg
     8941 tcaagacgct tctttatctt tctgtatacc aattagaatt tttagataaa gtccctgcac
     9001 atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa aggaaatcct gggacaggga
     9061 aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg agcaccagat ctgcaaggca
     9121 ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct gcctctatgg atgacgaaac
     9181 gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct cggtttatcg ctatatcagc
     9241 ctagccatgc tagtgcccga gtagatacac gccgactttc cagagatcag gccatcactg
     9301 ttttgcaaga agaagagatc acagctgaaa aaagccaagt cagtccatac ggtatagtag
     9361 cgaaaaaagg gcatttagct tctagctcat tgttccacga tggcgtcatg acgatccaag
     9421 atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga gcctgaacat catgtcttag
     9481 atgcctgtgc agcacccgga ggtaagacga cgcatattgc ttctttttta gatccgtcat
     9541 gcggtggaga agtggtcgct ttagatgtac acgaacacaa agtcaaactc attgaagata
     9601 atgcagcacg tctaggtgtc tcttctgctg tctacgctca aaagatggat gctagagaag
     9661 tcagtaaaca gttcgaagca gagcagtttg atcggatttt agtagacgcg ccttgttcag
     9721 gtcttgggct gcttcggcga aaaccagata ttcgttataa aaaacagccg gatgaattag
     9781 cgaatttacc aaaaatccaa ttggaaattt tagaaagctg cgcaccaact ttaaagtctt
     9841 ccggtattct gacttatagc acttgtacga tcttaaaaga agaaaaccaa gacgttgttt
     9901 ctgctttctt ggaaagacac cctgattttg agaggattga tgttttgacc aatcctatgc
     9961 tacaatcatc aatcgaagag aaaatgttga ctttgtatcc gcatcagttt tacacagatg
    10021 gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga aaaaatgcag attgaatatc
    10081 aatcagatgt aggaagacga cgtaacacaa atcaagacta tgccagtgta tttaccagcc
    10141 gttcaggtct caagcttgct gtattagcag acgggatggg tggtcatcgt gccggagaca
    10201 ttgccagtca gatggcggta acgaatctag gcgaagcttg ggaaaaacaa gaagctgttg
    10261 acgatgaaaa aatcgcccaa tggttcattc aatcgattca agaagaaaat atgctgattt
    10321 atcagcgtgg acaagaacag ccggaatata acggtatggg aacaacaatc gtagcggccg
    10381 ctctgtcaga tgactggttc acgattgcca atgttgggga tagcagagct tatttgattc
    10441 gtgaagataa aatcatgcaa ttgacggagg atcattcttt agtcaatgaa ctagtcaaat
    10501 caggagaaat cagcgaagaa atggcagtga accatccaag gaaaaatatt ttaacacgtt
    10561 ctgtcggcat gcctggaaca gtcgaagtag atgtttctac tcatctttgg caactaaaag
    10621 atcgactgct gttatgttca gatggcttga cgaacatgct gtctgaagaa gaaataggaa
    10681 cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac tgagctgatt gatcgtgcca
    10741 atgaagcagg cggagcggat aatatcaccg ttcttcttat cgaatataag gaggacagtg
    10801 tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa atcactggaa atatcggtag
    10861 cggtggtatg gcaaatgtat ttttggcgca cgatttaatc ttggatcgtg atgtagcagt
    10921 taaagtctta cgatttgact ttcaaaatga ccagactgcg attcgacgat ttcagagaga
    10981 agctttagca gcaacagaac tcgttcatcc aaacattgtt agtgtttacg atgttggtga
    11041 agaagacaac atgcagtatc tggtgatgga atacgtcaaa ggaatggact tgaaacgata
    11101 tatccaaaca cattatcctg ttccttatga aacagcagta aatattatgc agcagatcct
    11161 ttctgctatt tctttagctc ataaccatca aatcatccac cgagatttga aaccccaaaa
    11221 cgtactgatc gataatgaag gtgttgtaaa aatcacggat ttcgggattg ctatcgcttt
    11281 atctgaaaca tcgatcacgc agacgaatac gatgctggga tctgttcatt atctgtcccc
    11341 tgagcaggcc agaggaagta tggcaacgaa gcagtcagat atttatgctt tagggatcat
    11401 cctttatgag atgttgacgg gtagcgtacc gtttgatggt gaatctgctg taacaattgc
    11461 tttaaaacat tttcaagatg atcttccatc tataaaagca cttgatccaa atgtgccaca
    11521 agcactggaa aacgtgattt tacgggcaac ggctaaagaa cctgcagacc gttataagtc
    11581 agccgaggaa atgtctgatg atctttctac agtattatca ccagctcgtg caaacgaaga
    11641 aaaatggcag cctcatgtga tggataatga aacaaaagtt atcacacccc ttacggaaga
    11701 tgcaccaatg cctgattcat tcaaatctat gcctctgcca aaagacaagc cagaaaacga
    11761 aaccttgcca gaggaggaag aagttctgcc tgatcctaaa aagaaaggac agaaaaaatg
    11821 gtggctgatc ctattagcta ttcttgctgt tttaggaatc ggaacagctg tattctttgc
    11881 ttctggcggg cgaggagaag ttgttgttcc tgatgtttct gatctttctg aagcttctgc
    11941 tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca aaaacagaag aaattgcaga
    12001 tgacaaaatc gaagaaggta atgtagtgaa gacagacccg gcagcgggaa cgacagtcaa
    12061 gcaaaaacgc gaagtcactc tgtatattag tagcggcacg aaaaaaatca aattagatga
    12121 ttacagtggt aaaagttatg aagatgccga agcaagtttg aaagaattag ggttttctgc
    12181 tgacttgatt caaaaagaag aggaattcag ttcagaggta gaaagcggaa tgatcatttc
    12241 gcaatctcca tcagaagata cagaagtcga tcctaaaaca gacacgatta ctttcacagt
    12301 cagcaaagga ccagaaacag tgactttgag tagtcatgta ggagaaaact acgaaactgt
    12361 tcgtaattgg cttgttacac aaggattcaa tatcaataat attagtgcta cttatgacta
    12421 tagcgacact gtaccatcag gttcgatcat cagtcaggat ccagcctcgg gtgaagttgt
    12481 agcagaagaa acttacatca attttgtcgt aagccaagga acagagccta aattgaatga
    12541 tatctccggc tatacaaaaa gcgaagcaca aagttattta gctagtgtcg gtgcagaata
    12601 tatcgggcat gagacttccg aatattcaaa cacggtggat aaagataaag tgattcgtac
    12661 agtaccaggg gcaggaacga caattaccaa aggaatggtt gtgaacgtaa tttactctaa
    12721 aggaccagat ccagcgtctt ctagttctga aaaagactct tcagacagtt ctagcagctc
    12781 aagtagctcc agtagcacaa gcaactcttc aaaagaagaa tcttccagct ccacaagttc
    12841 atcagctagc acctcagaaa gttcaaaata agtgctgaat aaaatatatc agaagggaat
    12901 gcgacattga tttgttgcat tctctttttt gtgtttctat ctcttttgat caattttcaa
    12961 gccatatact ttgagaagtt tgcatggaat aacacctact ttcctcttcg tatggtaaaa
    13021 taaacaaaaa cggaggtggc acttatcaaa ggacaaatca gaaaagcaat cagcggtttt
    13081 tactatgtat acgcaaacgg agagacctat caaacaagag gaagaggaaa tttccggaat
    13141 cggaaaatca cgccgcttgt cggagatcag gtgatctttg agagtgaaaa tcaaacagac
    13201 ggctatcttc tggaaatctt gccgagaaaa aatcaattag ttcgcccacc agtcacaaat
    13261 gttgatcttg gaatcattgt cacaagtatc gtagagcctg cattttctta taatctactt
    13321 gaccgctttc tagttacctt ggaatacgag tcgatcgaac cgattatttt tttgacaaag
    13381 accgacttgt cagacgaccc agaaaaaatc gaagagatca agcaaaccta tactaaaata
    13441 ggctatccgg tgattgtacc caatcatacg ggtgatacga cagaactgat ccgttacttc
    13501 ccagaacggc tatcggtctt tatgggtcag tctggagcag gaaaatcgac gctactgaat
    13561 caaatatcac cggaactgaa tttagcaaca ggagagattt ctgatgcact tggaagagga
    13621 cggcatacaa cacgtcatgt ggaactgatt cctatctatg gcgggttagt agcagataca
    13681 ccaggattta gctcgattga ctttttgaca attgaagcag gagaactgcc gagacaattt
    13741 cctgaatttg tagaagcttc tgtccattgc aaatttagag agtgcatgca tgctaaagaa
    13801 cctggctgcg aagtaaaaca tagagtagaa acaggtggta tcgcacagac acgttacgac
    13861 aattatttgc aattcttaca agaaatcgaa aaaagaaaac caatgtatgc aaaaaaagaa
    13921 ggtaaacgaa aataggagga aacaagaatg aagatagcac catccatttt gagtgcagat
    13981 tttgccaatt tacaaagaga cattgagtta gtggaaaaag gaggcgcaga ttacatccac
    14041 gtagatgtaa tggatggcca atttgtccca aatatcacat tagggccaaa tattgttcag
    14101 gcaatccgtc ctgttactaa attaccgtta gacgtccatc tgatgatcgt tgatccagaa
    14161 aactacattc cagcatttgc aaaagctgga gcagatatca ttactgtcca cgtcgaagca
    14221 actcctcata ttcatcgggc acttcaaatg acaaaaagct taggagttaa agctggagta
    14281 gttatcaatc caggaacacc agtctccatg atcaaacatg ttttgccttt ggctgatcaa
    14341 gtactagtca tgactgtcaa tcctgggttc ggcggacaaa gctttattga agaaacagta
    14401 gaaaaaatta cggaactcgc tgatctacgt agagagaaca attggactta tgcaatcgaa
    14461 gtagatggcg gaatcgttcc agaaacagca aaaatctgta aagatgcagg ggcggatgtc
    14521 ttcgttgcag gatcatatat ctatggagcc gaaaatccag tcggacaaat cgaacgatta
    14581 aaagaggcat tagactaaat gaaaatttta ttagctgctg gaggtaatcc gcaaaactgg
    14641 ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga tagaccgtgg tgcgctgtac
    14701 ctgcttcaag caggctaccc agtagatctg gcagtgggcg attttgattc attgtcgaac
    14761 gaagagcaga aggaagtatt tgcaaaagcc aaagagtata cccgttcccc tgcagaaaaa
    14821 gacgatacag atacacagtt aggtatctta aaaacatttg aacgttaccc gcaagcggaa
    14881 gtcactttga ttggagctac gggcggaaga ttggatcatc ttttagccaa tctctggtta
    14941 ggtatggaac ctcgcttcaa accctttgcc catcagctta ttatcagaga ttgtcaaaat
    15001 tatttgtctt attatttgcc tggagagcat atagtaagac agataccaga tatgaagtat
    15061 ttagcttatt gctgcttgac tcccgtctcg aatcttacat taaaaggcag caagtatgta
    15121 ttggaaaata aagaagtaga atatccgaca tcttatgcaa gtaacgaatt tctttcagat
    15181 accgcagaat tttctttttc agatggtatg attgcagtta ttcaaagcaa agattgatcc
    15241 ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga cagaatctat tatttgctaa
    15301 aaaaaagctt aagaggagca acttaccttt tgtttcacgg catttagtga taaaattgaa
    15361 cacaagagtc taattaattc gaggtgtctt tgtgaagaag ttcaatccta ataaaaatat
    15421 tattattacc ttggttattg ttatcatcgt ggtgacgata ctaagtgtga ccgctgcgca
    15481 gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc gttaatgaca gtgttgcaat
    15541 ggttgataaa acaatctctg ccccagtacg gtgggtacga aatggagtag attctgtcca
    15601 taatcttttt tcaacttatg ccgaaaatga gcggctaaaa gaaaaaatcg atagctatga
    15661 tgacgtttca cagcaaaaca agaattacga gagagaaatc gaagcgttga aaaaagaact
    15721 tgagttgaat cagacgctga ctagttacga aaaagtaacg gcaaatgtca ttactcgttc
    15781 gcctgacagt tggcaggaca tgctgatcgt agacaagggt tctaaagacg gtatcgaagc
    15841 aaatatggca gtcatgtcgc aaaaaggatt gattggccga gtgatcgagg tcaatacggc
    15901 ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc tccaatcatt ttccagtacg
    15961 ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa aactatgatg aaaagctcca
    16021 tgccttagtg gtgacccaat taactggtga tacggatatc aaagaagggg atgttgtcca
    16081 gacatccggt cttggaggga attctccagc taacttgccg atcggtacgg ttattaaaac
    16141 gaaaccagat agttatgggc tggatcggga agtttatgtg aaaccttatg cagaaatgta
    16201 tgacgtgtca gttgtgacga ttgtccaacg gctagcggag gattagtcca tgttgaagaa
    16261 agaaacaatg aagtactatc tgccaatcgt tttgttcttt ttgatgttga tagatggtca
    16321 tttaacaaga atgctagggg agtggtcgaa aggcacctat atgtcaaatg cccactttct
    16381 gatattggca ttattatgtt gcagtatggc gtttgaaaaa cgttatttac tgattaccac
    16441 gattgttctc ggggctatct atgacgctta ctatattggc gttatcggta tctatgcagt
    16501 agctctccct ttaattgtat ggttgatgta tgtaatgaaa gacgttatcc atgtcaacat
    16561 ctttactgaa tttttcagta tgatcatctt tgtcacgggt tatgaattgt ttacgatggt
    16621 ggtccagttg atttttaaat tagcagtagt aaataacacg tattttatta caaggttttt
    16681 aggacctaca ctgctgttga acatgattat atttgtatta ttcatttttc cctt
//
LOCUS       DAEGIQ010000057        16729 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000057 DAEGIQ010000000
VERSION     DAEGIQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.057"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="KMT45_001691"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="KMT45_001691"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBE7005156.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="KMT45_001692"
     CDS             complement(2424..2675)
                     /locus_tag="KMT45_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBE7005157.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="KMT45_001693"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="KMT45_001693"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBE7005158.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="KMT45_001694"
     CDS             3624..3842
                     /locus_tag="KMT45_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005159.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="KMT45_001695"
     CDS             complement(4130..4642)
                     /locus_tag="KMT45_001695"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBE7005160.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="KMT45_001696"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="KMT45_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBE7005161.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7538)
                     /locus_tag="KMT45_001697"
     CDS             complement(7095..7538)
                     /locus_tag="KMT45_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBE7005162.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="KMT45_001698"
     CDS             complement(7539..8327)
                     /locus_tag="KMT45_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE7005163.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /locus_tag="KMT45_001699"
     CDS             complement(8324..9265)
                     /locus_tag="KMT45_001699"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBE7005164.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="KMT45_001700"
     CDS             complement(9442..10029)
                     /locus_tag="KMT45_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBE7005165.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11557)
                     /gene="obgE"
                     /locus_tag="KMT45_001701"
     CDS             complement(10250..11557)
                     /gene="obgE"
                     /locus_tag="KMT45_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBE7005166.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAQENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="KMT45_001702"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="KMT45_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBE7005167.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWVKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="KMT45_001703"
     CDS             complement(12946..13881)
                     /locus_tag="KMT45_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE7005168.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(14044..15138)
                     /locus_tag="KMT45_001704"
     CDS             complement(14044..15138)
                     /locus_tag="KMT45_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBE7005169.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            15327..16088
                     /locus_tag="KMT45_001705"
     CDS             15327..16088
                     /locus_tag="KMT45_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE7005170.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(16203..16643)
                     /gene="fabZ"
                     /locus_tag="KMT45_001706"
     CDS             complement(16203..16643)
                     /gene="fabZ"
                     /locus_tag="KMT45_001706"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBE7005171.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         4728 a         3678 c         2921 g         5402 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gaccacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt tgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataatgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttcctg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcac cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggt aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtgttg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgaatat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaatttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcaggg tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataacc tataaaaaaa
    13981 caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc aataactaat
    14041 tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa agtcaaggag
    14101 gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt ttttcatatc
    14161 cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt caatcagtac
    14221 atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt tgtagtatac
    14281 acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac tatgaaggtt
    14341 atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta ctttcaatgt
    14401 tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac ttgcgtacgc
    14461 atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca cttgtgtcgc
    14521 aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag gccaagggcc
    14581 aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt ttctgaattt
    14641 ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag gataaaaatt
    14701 atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt caacgtaatt
    14761 cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg tcatttttgc
    14821 ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca tcttatggaa
    14881 aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat tgatatcaac
    14941 cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt ctgaaagaac
    15001 tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat acccatactt
    15061 atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg gataaatcga
    15121 gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt gtatccactt
    15181 acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt aaaataaatg
    15241 ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt aaagataaca
    15301 aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta agtattagtt
    15361 tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact tcttctagtt
    15421 cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc agtagcgtcg
    15481 atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat gaagatctgg
    15541 tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa cacgcaccaa
    15601 aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga ctgactttcc
    15661 atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat ggcactggag
    15721 gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt tataatataa
    15781 gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc cagttcttta
    15841 tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat cctcaagcaa
    15901 tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat actgtcttcg
    15961 gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa acagatagtt
    16021 cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa gaagcaaaac
    16081 aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta ggattcaggc
    16141 gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat caggttttat
    16201 agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt tttatcatct
    16261 acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat ttcaaaatga
    16321 catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt gatcccaccg
    16381 atataagctc tttttcctag aaactcttct gattttagga taagtatcga acctgcttgt
    16441 gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa gtgaccttgg
    16501 aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc tggttctaaa
    16561 gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc cattacttca
    16621 gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg ccattttcat
    16681 tactttgact atcaaagtat catattccta aatatttagc aatcttttt
//
LOCUS       DAEGIQ010000058        16143 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000058 DAEGIQ010000000
VERSION     DAEGIQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.059"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..400
                     /gene="scm"
                     /locus_tag="KMT45_001707"
     CDS             <1..400
                     /gene="scm"
                     /locus_tag="KMT45_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBE7005172.1"
                     /translation="SDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSD
                     SSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGS
                     QSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(555..1541)
                     /locus_tag="KMT45_001708"
     CDS             complement(555..1541)
                     /locus_tag="KMT45_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBE7005173.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1564..2130)
                     /locus_tag="KMT45_001709"
     CDS             complement(1564..2130)
                     /locus_tag="KMT45_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBE7005174.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2144..2560)
                     /locus_tag="KMT45_001710"
     CDS             complement(2144..2560)
                     /locus_tag="KMT45_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBE7005175.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3017..4951)
                     /locus_tag="KMT45_001711"
     CDS             complement(3017..4951)
                     /locus_tag="KMT45_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBE7005176.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5232..6188
                     /locus_tag="KMT45_001712"
     CDS             5232..6188
                     /locus_tag="KMT45_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBE7005177.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6278..7381
                     /locus_tag="KMT45_001713"
     CDS             6278..7381
                     /locus_tag="KMT45_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBE7005178.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7394..8347
                     /gene="menA"
                     /locus_tag="KMT45_001714"
     CDS             7394..8347
                     /gene="menA"
                     /locus_tag="KMT45_001714"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBE7005179.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9161..10507)
                     /gene="gor"
                     /locus_tag="KMT45_001715"
     CDS             complement(9161..10507)
                     /gene="gor"
                     /locus_tag="KMT45_001715"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBE7005180.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10641..11894)
                     /locus_tag="KMT45_001716"
     CDS             complement(10641..11894)
                     /locus_tag="KMT45_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBE7005181.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11900..12823)
                     /locus_tag="KMT45_001717"
     CDS             complement(11900..12823)
                     /locus_tag="KMT45_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBE7005182.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13044..15536)
                     /locus_tag="KMT45_001718"
     CDS             complement(13044..15536)
                     /locus_tag="KMT45_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBE7005183.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15543..16010)
                     /locus_tag="KMT45_001719"
     CDS             complement(15543..16010)
                     /locus_tag="KMT45_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBE7005184.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4741 a         3370 c         2869 g         5163 t
ORIGIN      
        1 aagtgacagt acagatacta cctctaccac aagtgatagc tcagattcat ctaccactac
       61 aagtgatagc acggacacgt cagcttcaag tgacagtaca gatacatcct ctaccacaag
      121 tgatagctca gattcatcaa cttcaagcga tagtacagac acgtcgtcta ccacaagcga
      181 tacacgtcat gcaagtgtct ccttctcaaa aacaagttcg gataacagga cgaactttaa
      241 cttaactaat aaaacaagca atgacaatgg aaacaaatca aaaagagcac ttcctaaaac
      301 tggaagtcaa tcgaataatt ggataacact cgcaggcgtt atattacttg taatcggttt
      361 aagaatcact tttatgagtt ataagaaatc gaaaaggtaa aaaaatattc aagataaaaa
      421 agagcaacgt cgctaacgga cgttgctctt tatttttgct ttttatcata aatacaagaa
      481 ctacaattca atttgtcaaa aaacacgtag atttccttct gagaaattta tttacgtatc
      541 gaacctttcc ctattcagtc tttgcgatcc aaaatcagtt gggtaaggga aaagagattc
      601 tcttttgttc gaagagaggt ggcaggtaat ttctttattt ccttcaaagc cttttctgtg
      661 taagaggccg ctaacttcct agcttcttcc acgcctcctg tatgttcaac gattttgcgt
      721 acggcgtcga catcttcttc tgtcattgcc gctttcttct ctaaataagg tagtagcttt
      781 ggtttgtttt tttgcaaaga gtagatcaac ggtaaagaat agaccccttg acgcatgtct
      841 tctagtacag gtttgccgat ttcctggctc tcttttgtat aatctaaaat atcatcgata
      901 atctgaaagg ccatcccgat agaaagtccg atatctcctg ctttttttgc gaagcgctcg
      961 cttgttccac tttcaaaagc gccaacagaa caacttaatt ggaacagttc agctgtctta
     1021 cctgaaatat tttccaaata ttcttttaca gaaagttcat aattatagcg atcatccatc
     1081 tgacctaatt cgccattcaa tactttttcc atactgcgag aattcaattg gagacttttc
     1141 aaggaagtgg agtaatcaga caatagttta aaacagcaga caaataaata gtctcctgca
     1201 tagacagaaa cgacattacc aaactgacta cggatattag gctgaccacg tcgcagatcc
     1261 gcttcatcaa cgatatcatc atggatcagt gtggccgtat ggagcatttc gatagaagct
     1321 gccaaagcga tggctttgtc tcgatcttgc tctggtccaa attgtgagaa caatagctga
     1381 taggcaggac gcaacagctt tccgccggca tggatcgttt ctttgattgc ttgttcgaca
     1441 ggtttgtttt tcaggcggac agtttcttcc atccgtttta gtgtttctgt caattcttta
     1501 cttaaagcag gatagttttc ccacattgga tgtagcttca taaaaaaact ccttgtgtga
     1561 cgattatttt ttggtaaaca ttgggctcca tttttgtttg gttccctgct cataatcttt
     1621 ttggaatcgt tgaagtgtct gcacatgtcg taaaagataa ttcgctgcga tcccgattgc
     1681 aatccctgat aaaatcccta aaactgaaag gactggcaag tacaacatga ctgtccatga
     1741 ttgtgcaatg aaagaagcca tcaaaagctg tccgacatta tgcatgaaac cgccggccgc
     1801 actaattccg atgatactga cacgttttgg tcctaattgc ttgaccaata acattccaaa
     1861 ataactaagc aaagcaccac tcatactata aaggaatgtg gaaatcgttc cgcctagcaa
     1921 agtagtcagg aatagtcgta gccaaagtag taagaagctg tctcgttttt tcatggtaaa
     1981 aatggcaacg atcgtgatta gattggctaa gcctagtttt gcaccaggcg caaaagaaaa
     2041 aggataaggg atcatatttt ccaataaacc aatgatcact ccttgtgcaa caagtagcga
     2101 aatataaatc attttttgta atttactcat ggtgtgtgaa ggtctaataa atcagactgt
     2161 cttcgtcacc tccatctgtt gaacggactt cgatcactaa tttatgtggc aaacagacga
     2221 tggtttcgcc atttttcgtt gcccagcctc gacgtacgca gatctggtca tcgcaatctg
     2281 cttcttttat ccgtactcga tcgccatcta tctcgatcaa gttataatct ccgtgttcat
     2341 ctttgtaagt atatgtgttg ctctcttctc cagccatcaa agggaatgtt ttgatttctg
     2401 tgccatccac acgcagtacc gcttcttttt ctgctgttgc ggtgttttgc tgaagaccaa
     2461 aaatcatgac tggtaaaaaa gaaagaacag tcaaaagcaa aataataaaa acatcccaag
     2521 gtttgagcag acttttttca gcaaattctt taaggctcat ttttgttctc ctttttccag
     2581 attaactcac ccaattttag cataaaaaca agaggttgtg acacctgtgt cttgctttga
     2641 gtaataagaa cgaagaatag aaaacagttt tccatgtttt ctaattcttt gggcttatta
     2701 ccgagaagca gcaggtggaa caccgtttat aacaagaggt tgtgacagaa gtgggcaact
     2761 tcaagaaata agaaggaatt tacgaaaatt acttttcaag tttttgtgaa atttcggctt
     2821 atttttcgaa gaagctactt ctggaacacc gtttataaca agaggttgta acagaggttg
     2881 tgacacctgt gtcttgctca tggaggctta tctcgggcat cttattcctt taattttaca
     2941 taaaaaggag gctgtgacgg tttgtctcag cccctttcta ttgaaatctt gcattcaaat
     3001 aacgttcttt atctatttat ttcttgccgt actgggaact tggtctaccg taccaccaat
     3061 tacctagtgt acgttgtagc catggaatta cccagcgatc caacccaatc gcacgtccgg
     3121 aaccgttcat caatgcgaaa gcaacaaaaa tgaaccaaat gtttacccag taaaacatgc
     3181 ctgataagca gaacgtgatc accaatacaa tcgttgtcgc actgcatagc catgtgaata
     3241 atccagcaat caatgctaat gccaaggcaa tttcaaagaa taccatgaat ttttggaaga
     3301 acattgctac atcattgttt ggcatgatca cttgcatgat ctttgtgaac cagtctggtg
     3361 acttctcaat cacagccatt ggttcttcac cgtatgcata actcaaacca aaatgtgcag
     3421 cttgagtcgc tgcttctccg gcacttgccc ctgctccgct ggcagctgtg gttgcatcac
     3481 cagcagcaga agcaccactg gcaacatctg ttacattgta ttcccatgtc caagggaaca
     3541 cactgtttcc agtgaaccat gatccttcac caaaccaagt acttggtttg aacacttcac
     3601 cattcccaat gatttttttg gaagcttcaa cgaaccaaac taaaccatag aaaatacgaa
     3661 gtggcacact ccataagaca ttgccgtaac gagaagaatg tcctcgagtc acgttgcgat
     3721 catctttgat atggaaaaac tcatgcatta aatattggaa catataataa ccagaacgaa
     3781 tatcaaagaa gtatttcaaa ttcacgatat gtttcattac gatagctaaa aatccgctta
     3841 aatggatctt atcaaatagg ttagctaccc cccatttggc accaatagaa accataaatc
     3901 cttgatagtt tcctttaaat ttatgctttt ctccgccttt gatcgaagcc acgatattcg
     3961 ctgcggctgt gtgtcctgtt tgttcagcag cttgaacgat ctgcggtgtc ggtgttcctg
     4021 ggaattcttc ataaaagact aaatcaccaa tgatatagat gtttttatct tcgtagcctt
     4081 ttgcctgcat atattcattg gcgatcaacc gctgtccgcg tgctgattct aatccaaagt
     4141 cagcagcgtc agaagttcct ttgacaccag ctgtccaaat cagtgtgtga gtcggaatgt
     4201 tgcgtccgtc tttcaatttg atatgatcag cagccacttc tacgattggt gcattcaata
     4261 ataattccac gttgtgctta cgcaaatagc gttcagcttt tgcagcatca tttcgatcca
     4321 acatattcaa gatcgtcggc attgcctcta cgaccatcag tgtgatttcg tttggatcta
     4381 atttataatc tttggctagg cgatttttcc agtcgatcag ttccccaatc atttcaatac
     4441 cggtaaatcc agaaccacag acaacgaaag tcagcatggc tttacgcagt tcatcatctg
     4501 gttccaatgc agcttttgct actgtcttct caatatgttc ccgaatcctt acagaatctt
     4561 caaatgacca aagggtgaat ccgtgtgctt tgacaccagg tgtaccaaag tcattcggct
     4621 ctccgcccat tccaaggatc agctgatcaa aaggatagga acctaatttg gtcttgacga
     4681 tttttcgttc tttatcgata ccgattacag tgtctgtgac aagttgtaca ttcttttgac
     4741 gagcaaacaa atgctgcaga tcgtattgga ttgcttctgg ttctacacgt ccgccagcta
     4801 cttcatgcaa ctcggtcatc atagtatggt acgaatgacg atcgatcaga gtaattgcta
     4861 catcttcttt tttcagtttc tttgccaagt attttgtagc tgaaacaccg gcatatcctg
     4921 ctcctacaac gacaatattt tgctttgcca ttgatcatcc gctccttata gactatctat
     4981 ataatgaata tatgataatt cttttagttt gatacttatt acacaatctc catatattat
     5041 atagatgttc tgttcttttg tctaactcct tttggatttt tttcaaattt taaaataaag
     5101 cgttttttca cgaaatttgc tgaaagaaaa cgtttgaaat attacatggg ttatttatca
     5161 aaaatagttg acttataggc taaaaagatt agaatgtata atgaatcaca aagaaaaatt
     5221 gtggactaga aatggaatgg tggggtatta aaatgaagaa aaaacgtttt gtaactggat
     5281 ttgccgtatt agcattttca gctctagtgt taggcgcttg cggcgcagat gacaacggaa
     5341 gctcaaactc ttcttctgaa tcatctacag cacaatcttc gactgcaaaa agcacaacag
     5401 aatcatctgc aaaagttgta gctggcggag atttgcaaga cggcacatat aaattagaag
     5461 aaaaaaatta ttcaaatggt taccgtgcag tctttgaaat ggttgtaaaa gacggcaaaa
     5521 tcactgaatc aaaatatgat aacgtcaatg aaaacggcga atcaaaaaca aaagatgcag
     5581 aatacaacaa aaacatggaa gcaaaatctg gcacaaaccc agaaaaatac attcctgagt
     5641 tgaatgacca attcttaaaa gcacaaagcg caagcggtgt ggaagtcgta actggagcta
     5701 ctcactcatc tgaaagcttc caaaactacg cacaacaatt gatccaagct gctcaagctg
     5761 gtaacacaga tacaatcgaa atcgacaatg gtgctgattt gaaagacggc acttacaagc
     5821 ttgaagaaaa gaactactct aacggctacc gtgtgcaatt cgaaatgaca gttgctggcg
     5881 gcaaagtaac tgaatcaaac tttgattata tcgacaaaga cggtaaatca aaacaagatg
     5941 atacagaata taatgaaaat atgaaagcaa aaagcggtac agaaccaaaa acttacatcc
     6001 ctactctgaa cgacgaattc gtcaaagcaa tgggcgaaga agatggctca cctgctaatg
     6061 tagaagttgt tacaggtgct acacacagct cacattcatt catcatgtac gcacaacaat
     6121 tagtgaatgc agctgaaaaa ggcgacactc aaacaatcga agtagataat atcgtaacag
     6181 aaaaataatc acataatcat actaataaaa gttggagggt gggacgatag ccagattagg
     6241 cagtcgtctc acctttcaaa atgaagagag gaaaaagatg cggaaaaaac tactattttc
     6301 cttaatcaca ctgggcatct cttttatggt gctaactggc tgcagcaact cagcaaataa
     6361 aacaaccact gaaagttcag cagctaccaa gatattgaaa gatccatatt ctgatgaaca
     6421 gttcttgtta gggacttacg ttcgtatccg tgtgtatgat gaaggcaaag aatcagcatt
     6481 gaaacctgcc ttcgatcgag taaaagaact tggtgataaa atcacgatca atcaaaaagg
     6541 ttctgagatc gatgaagtga atgagcaagc tgggatcaaa ccagtaaaag tttctgatga
     6601 cgtctacacg ttagtcaagc gggcctacga atatagccaa gattctcaag gcggattcga
     6661 tatggcgatt ggtgcaatca cacaattatg gcgaatcggt tttgatgacg ccagaaaacc
     6721 ttcccaagaa gaaatcgatc aggcattgaa attagtggat tatcataaga tcgaactaaa
     6781 tgacaaagaa aaaactgttt atctaaaaga aaaaggaatg atcatcgatt taggtgcaat
     6841 cgccaaaggt tacattacgg acgaagtagt gaaagtcttg aaaaaacaag gagtgaccac
     6901 tgccatcgtt gacttgggcg gtaatgtcta tgtcttaggc cacagcccaa gaggcgaaaa
     6961 ccaagactgg acagtcggca tccaagatcc gaacatggct cgcggatctg ttttaggttc
     7021 tattaaagaa cggaacaaaa cattagttac ctctggaatt tacgagcgtt atttagaagt
     7081 agatggcaaa aaataccatc atttgtttga tccaaaaacg ggttatccgt tcgacaatga
     7141 tatcgcaagt gtcacgatca taacagataa atcaattgat ggtgacgggt tgtctacagc
     7201 agtcttttca atgggtgtga aaaaaggact ggaatacgtc gaaagcgaat tgaataacgg
     7261 gacggaagcg atcttcgtga caaaagacga taaagtatat gtgaccgatc caatcaaaga
     7321 caccttcaaa ttatcggagg actcccatta cacgatgggt gaccgttctg aactaaaata
     7381 aggagtacga accatgacac taaaaatctt tttagaagta gttgaaatac gaacaaaggt
     7441 ggctagtgta tttccttttg cggtaggggt attattctct atcgcttttt ttcatgagat
     7501 acattggcta aacacttttt tattttttat tggaatgcta gtttttgatt tagcgactac
     7561 cgctatcaat aattacatgg attatgaaaa ggctcattct gaagtatata agtatgaaga
     7621 aaatgtcatt ggccgcagaa atctatctcc tgtattgatt cgaaacatga ttttagctat
     7681 gattgcattt gtttttgtta tcggcgttat tctaacgatc aatacgggtt ggcttttact
     7741 tttgatggga tttatctgtt gttttattgg agtattctat acattcggac cgattccttt
     7801 atctcgaatg cctttagggg aaattttcag tggggtgact atgggccttg gaatttttgc
     7861 catggtcgtt tatgtcaata cattcggttc agatgtctat gatctgacag tcaacttttc
     7921 atctggacaa tttgctttgg taggaaatct ttgggctata gcagcaatcg tattagcttc
     7981 cttacccttg gtgtttacga ttgccaatat catgttggct aataatttgc gggatcttga
     8041 ccgtgatatt gaaaatcatc gctatacact agttttttat atcggacgtc ctgcgggaac
     8101 agtcttattc cagctattga tgtatgcttg ctatttcgtg attttaatgg ggttgttgag
     8161 cggtatctat gaatggccga tcttggtcac cttcttgacc ttgccaattg tgaagaagca
     8221 tttggatcaa ttcaagcaaa cgttacccca gcctatcagt ttcagctatt ccatcaaaaa
     8281 tatggtctta ttcaatgcca gttatgtatt ggggctatta ctaacaatca ttatgcaagc
     8341 catttaacag catgaagagg ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc
     8401 aaataaaaat agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct
     8461 tttgaacacc gtttatcagg aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa
     8521 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg
     8581 cttttgaaca ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa
     8641 acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc
     8701 tgattgaaca ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc
     8761 ccttttttca cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt
     8821 aactatatgg tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc
     8881 acactttttt aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa
     8941 tattttgaac ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga
     9001 taatcaaaat ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac
     9061 ataaataact aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg
     9121 gtttgttcaa taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt
     9181 cttccgctcc tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca
     9241 tcttgatagc cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta
     9301 agccgacgat tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat
     9361 aatcatttaa tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt
     9421 cgccatattt ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg
     9481 taaagacgac tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata
     9541 aacgttctga taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga
     9601 tcacatcacc aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga
     9661 taaacccttt tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg
     9721 gtacacgtcc accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata
     9781 gcaccgtata ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt
     9841 ttattccagc ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc
     9901 taagtggtcg ttcttttcga tatgcccaca tgacttcact acctaaacca caaaatacac
     9961 ctgaaagttc agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg
    10021 ttaattcaaa aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac
    10081 ttgcacggcc tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt
    10141 cgatcgtttg actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat
    10201 ctaatcctct ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta
    10261 gaccggcgaa atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt
    10321 caattgtttc caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt
    10381 tgacacatgt tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc
    10441 ctgctctgtt tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata
    10501 tttgcatttt gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt
    10561 ttctacagaa aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca
    10621 gggatatatc agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc
    10681 tgtgcgactg gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct
    10741 tcttcattat gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat
    10801 tcgatttcat ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc
    10861 tcatcataag ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg
    10921 aataattgtt cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt
    10981 gctactttcc ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc
    11041 atataactgt ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat
    11101 ttcattcttc cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt
    11161 tcgatcattg acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca
    11221 gttaaactgt ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa
    11281 gattgagaac atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat
    11341 cgaccagaat aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta
    11401 tttttacgaa tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc
    11461 aacccttctt ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat
    11521 aaatggatcg gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa
    11581 acaatcactg cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct
    11641 tcatttcctt catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt
    11701 gcaaaagcaa ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa
    11761 ccatatgcat tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac
    11821 ttttccagtg ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc
    11881 atttctttca tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat
    11941 ctaattcacg gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt
    12001 cttgatccca gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt
    12061 ttacgaattt ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct
    12121 gataaagttc attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc
    12181 catgactatc ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa
    12241 gacctctttc acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca
    12301 gtggacgttt ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat
    12361 cgagtttttc ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc
    12421 caactgcgcc ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag
    12481 catcataaat atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg
    12541 tatcaccaac tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta
    12601 catttttcat tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat
    12661 acttgtgtgc taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac
    12721 gcaatccaaa ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt
    12781 ctgcttgttc aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca
    12841 acttcataat agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga
    12901 tttttgaaaa ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata
    12961 aaaagagatt gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa
    13021 attcagctac acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga
    13081 acattcaatg tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt
    13141 tctccgccaa gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg
    13201 attggacgtg caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat
    13261 ggtgtgattt tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg
    13321 atttggacga ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg
    13381 attcgattca agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc
    13441 atggctttgt gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga
    13501 atttgtgtcg ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt
    13561 ctacctttag aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg
    13621 tctggatgtg ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt
    13681 ttttctgtca actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat
    13741 cggcttgtac tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca
    13801 cttccaaaca tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt
    13861 ccaaggaaca tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg
    13921 cggatcgcac gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga
    13981 agtccttcca gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca
    14041 ccagtccact ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct
    14101 tgctcctgtt ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct
    14161 tgtctgcgta attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct
    14221 tgtactagtt catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga
    14281 tccaaacgga ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga
    14341 cgagaagtga tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc
    14401 tccactccat ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct
    14461 tcaggagttg gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt
    14521 tcgatatatt tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga
    14581 gctaacgccg gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca
    14641 attaaggtat gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg
    14701 atgacctttt tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat
    14761 cccatatcaa gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg
    14821 attttttgag ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt
    14881 acagggttat tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa
    14941 cgaccaacaa ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat
    15001 gaatctagcg taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta
    15061 cgacgattca ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact
    15121 tgacgggctt ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt
    15181 tcatctttta ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc
    15241 ttcacttcgc tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag
    15301 actcctttag ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa
    15361 atttccgttt cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg
    15421 accactaagg ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct
    15481 tcttcttgtg caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa
    15541 tctcacttcc catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt
    15601 gtatgatctt catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag
    15661 tttccttctt tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca
    15721 tttttctcac tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta
    15781 tctgaaatcc ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact
    15841 agataaccac gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca
    15901 cagttaaaga ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga
    15961 attttcttta tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt
    16021 ccctttgact aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaccg
    16081 ctttttgtta aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt
    16141 atc
//
LOCUS       DAEGIQ010000059        15900 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000059 DAEGIQ010000000
VERSION     DAEGIQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.060"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(52..522)
                     /locus_tag="KMT45_001720"
     CDS             complement(52..522)
                     /locus_tag="KMT45_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBE7005185.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="KMT45_001721"
     CDS             complement(681..1139)
                     /locus_tag="KMT45_001721"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBE7005186.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="KMT45_001722"
     CDS             complement(1310..2455)
                     /locus_tag="KMT45_001722"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBE7005187.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="KMT45_001723"
     CDS             complement(2468..3385)
                     /locus_tag="KMT45_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBE7005188.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="KMT45_001724"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="KMT45_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBE7005189.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="KMT45_001725"
     CDS             4477..4647
                     /locus_tag="KMT45_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005190.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="KMT45_001726"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="KMT45_001726"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBE7005191.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="KMT45_001727"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="KMT45_001727"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBE7005192.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /locus_tag="KMT45_001728"
     CDS             6911..7792
                     /locus_tag="KMT45_001728"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBE7005193.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /locus_tag="KMT45_001729"
     CDS             7789..8505
                     /locus_tag="KMT45_001729"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBE7005194.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="KMT45_001730"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="KMT45_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBE7005195.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="KMT45_001731"
     CDS             9222..10223
                     /locus_tag="KMT45_001731"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBE7005196.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="KMT45_001732"
     CDS             10220..11581
                     /locus_tag="KMT45_001732"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBE7005197.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="KMT45_001733"
     CDS             11541..12692
                     /locus_tag="KMT45_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBE7005198.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="KMT45_001734"
     CDS             12667..13836
                     /locus_tag="KMT45_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBE7005199.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="KMT45_001735"
     CDS             14011..15390
                     /locus_tag="KMT45_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE7005200.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="KMT45_001736"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="KMT45_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5202 a         2803 c         3384 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAEGIQ010000060        15864 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000060 DAEGIQ010000000
VERSION     DAEGIQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.061"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            68..751
                     /locus_tag="KMT45_001737"
     CDS             68..751
                     /locus_tag="KMT45_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBE7005201.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="KMT45_001738"
     CDS             753..1589
                     /locus_tag="KMT45_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBE7005202.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="KMT45_001739"
     CDS             1600..3354
                     /locus_tag="KMT45_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005203.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="KMT45_001740"
     CDS             complement(3630..3971)
                     /locus_tag="KMT45_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBE7005204.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="KMT45_001741"
     CDS             complement(4039..6210)
                     /locus_tag="KMT45_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBE7005205.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="KMT45_001742"
     CDS             6379..7278
                     /locus_tag="KMT45_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBE7005206.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="KMT45_001743"
     CDS             complement(7349..8206)
                     /locus_tag="KMT45_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBE7005207.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="KMT45_001744"
     CDS             complement(8191..10893)
                     /locus_tag="KMT45_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBE7005208.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="KMT45_001745"
     CDS             complement(10906..12195)
                     /locus_tag="KMT45_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBE7005209.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="KMT45_001746"
     CDS             12345..13391
                     /locus_tag="KMT45_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7005210.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="KMT45_001747"
     CDS             13393..15546
                     /locus_tag="KMT45_001747"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBE7005211.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4507 a         3308 c         2927 g         5122 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgag tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccatttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg ctttccacat tttt
//
LOCUS       DAEGIQ010000061        15825 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000061 DAEGIQ010000000
VERSION     DAEGIQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.062"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            490..1293
                     /locus_tag="KMT45_001748"
     CDS             490..1293
                     /locus_tag="KMT45_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBE7005212.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1878..2333
                     /locus_tag="KMT45_001749"
     CDS             1878..2333
                     /locus_tag="KMT45_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005213.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2452..3510
                     /locus_tag="KMT45_001750"
     CDS             2452..3510
                     /locus_tag="KMT45_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HBE7005214.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3552..5501
                     /locus_tag="KMT45_001751"
     CDS             3552..5501
                     /locus_tag="KMT45_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBE7005215.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5504..9145
                     /locus_tag="KMT45_001752"
     CDS             5504..9145
                     /locus_tag="KMT45_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005216.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            9545..9883
                     /locus_tag="KMT45_001753"
     CDS             9545..9883
                     /locus_tag="KMT45_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005217.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
     gene            9993..10235
                     /locus_tag="KMT45_001754"
     CDS             9993..10235
                     /locus_tag="KMT45_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005218.1"
                     /translation="MVFDREKMLAHANEVLMSGLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTIDRKKGVLQ"
     gene            10235..12397
                     /locus_tag="KMT45_001755"
     CDS             10235..12397
                     /locus_tag="KMT45_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005219.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYQKEEKEAKAKK
                     AKVKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEEVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLLIPDDPNMFESEVEEATDFYSAVEQLEVATN
                     GLERLAETFNSSEEVEFEALAEDIPDEKEALFADDDKPTEAENQEVSIEETQTQIQTE
                     TYQQSKIDEKPESQSDKENYVYEVEKIVSESDVPKSLDADFLEKNFEQIIADKFQPVV
                     LDEINDPVDLNEEMLSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGK
                     QMLADELLRIEQATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIENSLTDVQEKTLEIVEKTKSENEAEFHDTKEKITDVVVK
                     YFANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYRSNSEKLAEQS
                     EALREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTIL
                     GSSYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVIS
                     IDGKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            12409..12642
                     /locus_tag="KMT45_001756"
     CDS             12409..12642
                     /locus_tag="KMT45_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBE7005220.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            12632..13498
                     /locus_tag="KMT45_001757"
     CDS             12632..13498
                     /locus_tag="KMT45_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005221.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            13513..15483
                     /locus_tag="KMT45_001758"
     CDS             13513..15483
                     /locus_tag="KMT45_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005222.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            15650..>15825
                     /locus_tag="KMT45_001759"
     CDS             15650..>15825
                     /locus_tag="KMT45_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005223.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         5861 a         2223 c         3192 g         4549 t
ORIGIN      
        1 caaagttaca caagcaaaat aaactcctag atagtgttag ttttttttaa gattttttat
       61 ttttaagatt ttgaaataag agaggagtaa tcctccttta ttaaatatct gttaatatat
      121 ctgttaaata tctgttaata tatctgttag gggaggctta atctcttggg agagtaagta
      181 ttaaaggggt ataaaggtaa cacttttgga cagtaaaggt aacaaaagtg gacagtaagg
      241 gtaacagttt tggacagcaa aggtaacaaa agtggacatt tgaggtatca cactaggata
      301 agacgttacc ttcggttttc ttgatttgga aggcgaaaaa atagaaaatt agattagtaa
      361 caaatctttg ttgactgtta cctttagttg tgatttaatt taggtaacaa gcagaaaaaa
      421 ttgtttcctt taattagtac cagattaaca tgattaaagg agcgcaaaaa gaagattgga
      481 gagtaatcaa tgtctcaaga agttaccgaa aagattattg atagtgatgt agatttagta
      541 tcagaacaaa caaatgcaat tgtgcgttat aacaatggtc tgaatacaac agctttgcga
      601 ggatttaagc ctgttgaaat ggatgtgttt tgggctattt tatcaaaaat gaaacgtcaa
      661 ggaacagcag aagtaacttt tgatttcaac ctctttcgtt atttggtcaa atacgacaga
      721 agagaaaagg acagttttta ttctgtgtta aaagatttga gtgacaaact aggtaattta
      781 acctaccgtt ttgaagattc agacgtctat gaacagctgt ggctttttca aaaattccgg
      841 atagataaac agaaggaaac agttacaatt aaggcaaatg aggaatttga atttatctta
      901 aactcaattg gcaaaaactt tactcgtttt gaacttgata attttacaaa actaagaagt
      961 agctacacaa aagaattgta tcgtcatctc atgcaataca gaaataaaga gtggcgaaat
     1021 ggttattggg tagtcaaagt tgaagatttt agaagagcat taagtatacc ttcaagttat
     1081 agaatgacaa atattgataa aagaatacta gagcaggcga aagaagaatt tttagctcct
     1141 gatgaaaacg gaatacaggt atttgaaact tttgattatg aaaaaatcta tgcaaagaaa
     1201 ggaagaagag ttgatcggct cagattcact tttagtgagc cagaatcaaa cttgccaacc
     1261 atttcaatgc ataattggtt agaagaagat tgagaagaag caggttgtaa ttttaataca
     1321 acctgcttct ttaaaaaagt atgggtaaca cttttggaca ataaatgcaa gaagtttttt
     1381 tgtaagcaag tttcagtcta aataatattt gaaagaatgg ctaatctata caaattttaa
     1441 caactatggg taacactttt ggacaagtca agaaaaaaaa caatttttct taatttgttg
     1501 atcgactaaa ggtaacactt ttggacaaca cagaataaaa agtccggtga tatcagtttt
     1561 ggtaggaaac atcttaatat ctttgtgaga ttgaaggtaa catttttgga caagtcaact
     1621 atttgttaaa agttaatata gagtaattag ataagttttt tcttttcgtc tgataaatgg
     1681 ataatattag ttattttatt gctaattagt ctttcatgat gactgaaata gctaaaataa
     1741 atatctcgtt ggataattgc tgatacttta agggatatct aaatttgctg attgatttat
     1801 ttatgttatt atgatattat aataacataa atgtgttacg cccgaacctc attttaaaaa
     1861 aatgaagggg tgagacaatg aatgctatta attggcatga atttgttaac tatatggaga
     1921 gcttactaga tgatctaaaa agttttagta ttgctgatgc agtaaagcat gggttaccag
     1981 ttagtacaac gatgaaatta cgaggattga aaattccaga tgaaattatt aagctgagaa
     2041 ctaaaacaat tattgaaacc gcaaaagtcg ttgacaatat gaaccatgaa ctaaaaggca
     2101 agaaaacaac ttacgagaaa gattttgaac gttttttaaa atctgttatt ggaaaatatc
     2161 taggtaaaaa atctttcagt gaattgagag atgaagagat tgtacctact ccaatggtta
     2221 gcaaaatccg aaaaaacaaa gccgagggga attactatct tggtacaata gcaaaaatca
     2281 ttgattattt ggaaaataaa gagcaaaaga aaggaacgaa gaaaggaaga taatttttgt
     2341 gttaaatatt aatggggaag aatataccga aatgattcaa aatcccgaac ctagaattac
     2401 cgaaaaaacg tctgattgtt tgctctttta tgaaaggggg cagacaaact aatggaaaaa
     2461 aacgtcaatt caacatctac caaagatcaa tacagtatta ttggagggtt gcttggtgat
     2521 aaggataaga gaacacgtca cggattacgc attatgcttg caacttttct aggagtgctt
     2581 tttttattaa caggcttggg tagctacatt tatcaattag gaaactataa ttattatctc
     2641 gtgaacaata caacacctat aggctcttca ttggagtttg ctcgatcagg agcagagatc
     2701 actatttcag atgtgtggac agatcaaaac agagatgtaa cagtagtaaa actcggttac
     2761 tcgcaaaacg ctagaaagtt gttatctacg caaggaaaaa actacaactt atatatggca
     2821 acgaaagaaa aagatcgtcc taaaaagctt gaaatgagct atgggatgtt aagtactgat
     2881 ggggatgctt tcttattcct aaaaggtaaa ttagatgaaa gagcttatca agtttttatt
     2941 gcaaaccaag tatcactaat ttctggtgat gatacagctg acgatgatac caaagtcaat
     3001 gtgaatgaag cagattcagt tacaaaagca ctttctcaat attctatgaa tgatgttaat
     3061 aatgaaggag taatttcttt tgggaaaaaa gcaaaacctg ttaatactcc tgataatatc
     3121 aatttcagaa taaatccata ttcagattca actaatgtct atcaaggaag ctttcttacc
     3181 aaagatggcg atattgatta ttcaaaagtt gtagcagtta caagtaagaa taatgttgta
     3241 agtaagttag aagatcagtt aaatgcttca aatactaaca tcaagaaaat tgaagctaca
     3301 aaagctgagt atctggaacg tttacaacaa aatcctgacg ataaagattc gcaagaaagt
     3361 attcgtcaac tagacagtcg aatggagacc gaaattaaag caatcaatga tacacaaaaa
     3421 ttgattgatc aatacaaaaa tgctgacttt aacaaagatt cttttggaga catgaagacg
     3481 acatttaagt acctaactct gactgactag aagtaaatta ttcttatttt agaggaagtg
     3541 aacacaaaca gatggaaaat agtaccgagt tttttaagaa tattcaaact gaatttaatc
     3601 atgctctcac accaatgaaa ccaccgaaac ataagttagt gggacgtgat gacgaactcc
     3661 atgagttgtc actgatcttg aatagaccgg aaacaccgca agctatcttg ttaggtgaag
     3721 ccggaacagg taaaacagca cttgcaagtg aatgggctga aagacagcga caagatggaa
     3781 agaaagtaca agtatacaaa ttatcaatcg gacaattgcg tgttggggga gatgatgtcc
     3841 tccaaacacg tttagaaaca ttaattccaa gattgaaaga atatgagcga attttaaaat
     3901 ctgaggacaa agaaaatgaa tttgtcctct tcattgacga gtttcataaa gttgttagct
     3961 cttttggtag tggcaataaa attgggggtg attcgctaaa agaaccgtta gcaaatgaat
     4021 cacttcaatt gattaccgct actacaagaa aagaatatga aaactatatc aaaagagatg
     4081 atgccttagg gagacggtta caaccgttag aattgaatga actaaaaagc gatgttgtcg
     4141 tcgatatttt gagacaatgg ctaaatacat cctctgttaa agatgatcct caggcttctt
     4201 ttaaattatc gcaaaggatt cctgatgaat tacttcaaga aatcgtacaa gcaaacaagc
     4261 tttatcgtga ggatttgtcc gaaccggcta aatcaattgc cgtaatggag gatttgattg
     4321 caaaagatcg agtagacgga aatgcttata atcgagaaag tctattgcaa atatttggta
     4381 agtataatat tcagctagat tctgattttg atatggaaaa acttcaagag gaattaaagt
     4441 ccataaaagg gcaaccgtta gcagtttata ctataacaat gttgtttaaa cgtatgaagc
     4501 ttggatttga tcgttttaaa ggtcgaaagc aaccaaaagc aactgctctg tttacaggat
     4561 caacaggggt cggtaaaact gcaatggcta aagccgtggc aagaggactt tatggcgatg
     4621 aaaataaagt tctcttagtt aatatgccgg actacaaaac aaaagatgat gcaatgcgtt
     4681 ttagacgact cataggtact catgtaaggc atgatccttc tgcgattatt gtgtgtgatg
     4741 aatatgagaa agctggtgta gaaacactcg atgccttttt atcaatttta gatgaaggta
     4801 ggataagttt tactgaaaga ggatgggatg atcgagaagt tcacgacact gtttcattgt
     4861 caaatacaat tttatttgcg acaacaaatg caggtgcgga gatgttcgat tcgttaaaca
     4921 gatatagctc actatctcgt gttacaaaca aggaaaattc taatgaatat agcactgaac
     4981 taaaaagaga ataccgaaaa gtcgcatcag ggatcaaaca agccttgatt ggtgaaggtc
     5041 taaagcctga agttattaac cgatttggac gaatagttcc attttgggct ttatccgaac
     5101 aaactcttct ggaaattgct tataatgaaa tgttgaagag tgctgaatta gtacgaaaac
     5161 atttaaatat agatttacgt cttccaccta ggaaagaaag agtatatggt caaaattttt
     5221 atgcagacga tctctccatg cacttagttt ttgacatggc ttcacaagag gattctaaat
     5281 cagggggtgc aaggaatatc ttgcatttag ttgaatctga gtttgaagaa gcgattactg
     5341 aagcaataat tacttatcca aacgcaaaaa tattcgatgt taagttgagt gaagccggag
     5401 cgtttgaagg aaaaggtgat tctaatgctg aaattattgc aaagccttcc gttgctgaaa
     5461 gtaaagaaaa caagattgta ttttcgattg gaggggcgta gctatgaaat tcaagaaata
     5521 tttactacaa aaccttagaa taaaacgatg ggtcgtaaga ggtctagtta ttggaggagt
     5581 attagcagtt actttaggcg ctccttttgg aatgaatgat ccaactcctg acaatcaaga
     5641 tagcaaacaa gaaacagtta taaataagaa cgttgcatat gcggaaagta aaccggcaga
     5701 acaaggaatg tggacactat atgctcgtat cctaatggga gataaagaag ttaatgatag
     5761 tgccgctaaa actggacaaa tgaatttgtt aggacaaggt ggtatcagtg ccgatttccc
     5821 ttattctgac atttctaaac aaggtaattc gattgcaggg ggtaatgcag gtactcaact
     5881 agcatctact ttagcaactt atagttacta cggatatatt gaaacagtct caggaaatgc
     5941 tattgccagt caagcatcat ccattctagg aagtattggt aggacgattg gtagttttat
     6001 cggatcaatt agtttaatcc tttctaatat tgctgaatcc ataaatgaac tccttggaaa
     6061 atttttcatg agtatgaata tattttctct tatggggtgg ggagaaggtg gatcggatgt
     6121 atctaatccg gtatctaaag cattaactag cttgcttaac aatttgggat taaataatga
     6181 attatttaaa gcgatagcaa gcttatctat gctctttttc acagcaatac taggttatca
     6241 aggaatgaaa ttattgtcga aagatggtgt taactcgaag ggattatcac cagtacttga
     6301 aaaatggatt gttcgtattt ttgtattgtt tatattctta ccagtggcag gatcgctatt
     6361 gggagtttat atcaacggca tccagtcagc aaaaaaagat ggagatatac caacaaatgt
     6421 tgtttctgac tacatcatta acacaagact ttgggcttct actcaaaatc ttgctccatc
     6481 atctgatact ggtggtgatg tacctgacgg aaagcctaca tccgaaatca agttcgttga
     6541 tacagattat gaaccatcta aaaaatctgc aatgataaga gcgatcaata ctagcagtta
     6601 tgccagacaa aataatgatc tagattctaa ttcagtcggt atggctctat taaaagaatg
     6661 gtcatcgaat gatactttca atattaatac ttatgccggt gatgttaatg tcgccgaaaa
     6721 tgatgcttat atgggaagag cgcctgctgt ttggacaggc tataatttag gggatggaaa
     6781 agaaggaagt gcaattaaga aaccaaccaa tttaaatgga tatatttggt cagcaaatca
     6841 aaatgtgaca gacgacaatc gcaagcctga tggaaaagga acagcgcata actttgatgc
     6901 tactcaacct tggggcgttg ctaacaatgc aacttttagc acacagtcgg tagctttatt
     6961 gctccaatcg gcacttgata catcgggagc taagttttat gcgtataata tcgctccttc
     7021 aggaattcaa ggcagtgcta agaatatgtc aacagtaaaa acagaatggc gtacagcatc
     7081 cttagttggt catggagcta tgggtaagtt tggctcgtgg ctagcgatga acgctgaatc
     7141 aatcgtttta gcagttcttt acttagcatg tgcttgggcg atgttcaatg tgaatatcgt
     7201 gaaagcattt aaacgtgaat ttattcggat ttgggaagta gttctaacag gatcacttgc
     7261 atcaggagca ggtgtgatgg caatttcatt aggtgttgtt acaacatctc tgatcggact
     7321 acaattacct atctttctga ttacttttat tcgtgcattt ggtgatactg ctgtgggatt
     7381 cattcctaat acattagttg gcgatggcgt agcaaatatt gttcgatctg ctatgatttt
     7441 aggtgtagga tatcttttag tctttaaaaa aggcgaaaaa gtaaataatt tgattaatat
     7501 gcttattggc ttaccacttg aagtagcgtt tgggtttgat gaacgtgcta agttattatt
     7561 tggtaataga aatgatgctg tgcgagttgt ggacggcgtt tctaaagcaa tcaagaaaag
     7621 ccaaggagct aaaaatccct attatgatgg tggcaacagc tcagtaaaag atatggttgc
     7681 aggaggtctt ggagcagttg gagcgcaaaa aatggctgaa cgtttgaaca atatgggaga
     7741 tagcaatgct gatgctaagt ccattgctga taaagtaatt cctgaaacac cagaaggaat
     7801 gtcattcaac ggtggcggag ttggttccac aatcaataat acctctttca aagatttagt
     7861 tcgtcaagat caactacgta ataactccca acaatatatt gatcatttag gacgtatggt
     7921 tgatgaaaac ggaaactttg tcgatgaaaa tggtgaaata ttaaaagata gtaaatacgg
     7981 taaagatgct aaaggcaact ttattgacga aaaagggcgt ttggtcgatg aagatggaaa
     8041 ccttatagat actaatggta aaccattgga agatagtgaa gatggtaata ttggtatgga
     8101 tagcgaagga cgtttaattg atgaaaaagg tcgcttagtc gatgaagatg gaaatcttgt
     8161 tgacgaaaat ggacgcaggt tgctaaatgg tgattttggt catgatagta acggacgttt
     8221 agttgacgaa aaaggtcgtt tagttgatga agatggaaat cttgttgatg agtttggtaa
     8281 taaacttggt aaacacagcg atggatataa tgtgaatgga gatttagtcg atgaggatgg
     8341 gaatatcatt gagacgagag atgcgcttgt ggcagatcga aatagagaac tcgaacaaca
     8401 acggcatacg ccaattgctg ttgcaattcc tgagaatgaa gaagacgaga ttactttaga
     8461 tcaaactcat caagaactga ggaatgaaat gagtggtgag gacgtgtcag acagagtgca
     8521 tcgggaatta ggagatggag catctggtga aagtcagtca agagagctta acagcaactt
     8581 tgataatagt gctattcgga atgacacagt acttaaagac agagaatcgc taacaaaaga
     8641 aacggataaa gaacaatcag atgtgaagaa aaagttcatt cctactccta atgttaatca
     8701 aacagataaa tatgaaacaa atattggatc aggaatgtcg aaagacaatc aatcaattag
     8761 agaacctgaa ctgacaaata aaaataatag aacaaataat tctaaatcta cttcagataa
     8821 ttcagggaaa tctaatcaat caaccaagaa aacatcaaca gctaaatcag ccgttgaata
     8881 tctatcaaaa actggtaaaa atgtttacac ctcagcagat aaagctttag caaaagctcc
     8941 aaaaggtagt ctagcaggga taacctatga agctaaaaac gaagctatta aaataggtaa
     9001 aggaacggct aaggttgcca aggctactgc tacaggagca tccaaaactg gtaaagtagt
     9061 gaagaaatct accatgtcag tcaaggacta tattgtaaaa tacggtaatc aaaaatcaac
     9121 tgaaaataag tctgaagacg agtaaaaaga aggagcattg atttgctcct tctttttact
     9181 ttgatttcta actgaagatg tcaaggaaaa tttctattta tgcaaaaaaa ttattttaaa
     9241 ttgtctataa atgaattaaa tatttactgt tcaaagttgt ataattaaaa agggcttatg
     9301 ctggtaaaga acagaattat tatctaaaca aaaacttgat aaaccagcat ttttagccaa
     9361 taaaaaaagt gaaataattt aatggaaaat ctcttttaat catcgaaaat ttatgttatt
     9421 atgataacat aaagatgtaa atatcgagac gtctttttct tagttagatc attgacaagt
     9481 gaacaaataa gatttgtcga tgatataatt aacttattaa aaatattaaa aaggaggttt
     9541 ttatatgttc gattctatta aaaatttggt tagcggaatg acgcctgata tgaaaacttt
     9601 agtacaagtt gttttgtatt tcttggcatt cgttttttcg ttgaaaccag gattcactgc
     9661 aatgaaattt tttggtgatg caaaatgggg agatggatta aaatatgttg gggcgattat
     9721 tgcaatcttt attattcctg taattacaac cgttatgctt tacacaatgg gtactaaaac
     9781 aggtaatgat ttgaaccaaa aagctaatat catatcagca cttattccag ttgttggtat
     9841 ctatctagtt tcaaaatttc caaaaaatga acagttaggt taaaagtgaa ttgaacaaat
     9901 ttggataata tgccgatttg ttcaatttac tttttaccac aaaactacgc tccaagcgta
     9961 gtttagaagt gagggaagaa agtaaatgta agatggtttt tgatagagaa aaaatgttag
    10021 ctcacgccaa cgaagtatta atgagcggtc tgaaaggtac cgaactggca aagataatga
    10081 atatgaatgt taaccagttc tatgattatc ggaatgggag taaaaagatt gaaaaagcca
    10141 gacttgaaac gcttattaaa ttcgaaaaag catatgtata tatgctagac aaacagaaaa
    10201 gaactattga ccgaaaaaaa ggagttttac aataatggca aaattcaaag atattaccga
    10261 atctattaat tatgtggcag aaacagttcc tgtagttgca ctctctttga agccacaatt
    10321 actcgaagtg taccaaaaag aagaaaaaga agcgaaggca aaaaaagcta aggtaaaggt
    10381 atggaaagaa ttcgatgcgt tgctgaccaa agatttacca aagaaaaatt atatggtaat
    10441 tgaagatgta caaaagctca acagtgagga agttatagat gaggatgatg ctattctcga
    10501 ttccgaaaag tatgtcgttc tattgaagtc aagtttgaat tatgtagctg atctaccaga
    10561 aaaatttaaa gagaagctat tagcaggtgt ccgttttatg gatagtagtg gtcaattatt
    10621 acttattccg gatgatccta atatgtttga atcagaagtt gaagaagcta cagacttcta
    10681 tagcgcagtg gagcaattag aggtagctac aaatggatta gaacgacttg ctgagacctt
    10741 caactcttct gaagaagttg aatttgaagc cttagcagaa gacataccag atgaaaaaga
    10801 agctcttttt gcagatgatg acaaaccaac cgaagcagaa aatcaagaag taagcataga
    10861 agaaacacaa actcaaatac aaacagaaac ctaccaacaa tcaaaaattg atgaaaaacc
    10921 cgaatcccag agtgataaag aaaattatgt ttatgaagta gaaaaaatcg tatccgaaag
    10981 tgatgtaccg aaaagcttag atgctgattt tttagaaaaa aattttgaac agatcatcgc
    11041 tgataaattt caacctgtag ttttagacga gattaatgat cctgttgatt taaacgagga
    11101 gatgctatcc atagctgagt tgaaaccatt gctgttttta gctaaagaga gtatgaatgc
    11161 tcgtatttct atcgctaata ataaaatcaa cagtgaatta aataagacga aggagcaatt
    11221 gatcctcaaa ggaaagcaaa tgttagccga tgagcttttg cggatagagc aagcaacctc
    11281 attaaatggt caaaatcagt ttacaaatac attatcggtt gctaaagaga gctacgaagc
    11341 agaagcatct aagattgatg atttagtgat ggaaaaatcc agtatcattc gtaaagatta
    11401 tgatatggcg aaacaggctt atttggatgc tgaactaaag aaactagagc aacggtatga
    11461 tgctgaacat ttaccagaag ttaaacaaca agaagatgaa tatagaaaag caactgaaga
    11521 aagtcttgat caacagtacc aagaagcaat tgaatctatt aaaaacgatg cattgaaaca
    11581 aaaccaagaa gcaaaagaag ttgtggtgga acggattatc aattcattga aaccagaaat
    11641 tgaaaactca ttaacagatg tgcaagaaaa aacgttagag attgttgaaa aaacaaagtc
    11701 agaaaatgaa gctgagttcc atgatacaaa agagaaaata acagatgtgg ttgtcaaata
    11761 ttttgcgaac aaagaagaag ttgaaaagca agtagctaac aacacgaaag aattagaaac
    11821 aaaacttgta gagcaacaaa aaactatcga agaatataaa gatcgaatga aagaggtcaa
    11881 agatttagca gagggttata gatcaaatag cgaaaagttg gcagaacaaa gtgaagcttt
    11941 aagagaagac ttatctcgtt cccgaagtga tctagctcaa atggcagaaa aaacgaaatc
    12001 attagcagtt gctacagaaa atcaatcaag ttcaaaacgt gattttacaa gtaaaaagat
    12061 ggctactatc atttctgtcg ctgcaatggc ttgcggaacg atcttgggat cgtcctactt
    12121 tattagtcat aataatcaac aagcacaggc tcaaactcaa acatctattt cgagagtgga
    12181 aaattcgatt gggacaaata cgaactctgc ttcaacaaat agtagctatg aagcaacacc
    12241 aactacagag ttcaaagttg ggaaagatgt tgtcatcagt attgatggga aaacaactaa
    12301 ggctgaagtg atttctattg atggagaaaa agcagttgtg aaaactccag acggaaatca
    12361 gtacaaaatt caagctcact tatctgataa taactaagga gggctattat gcgagaagaa
    12421 aaaatagttt atccagtaat tcaaaacaat caagaaaaca tgacctcttc tcagcactta
    12481 caggaagcac agctcgtgta tcaggtaaat gaactaccac aaacgaacga gacaatgagc
    12541 aagtcaatat ctttaattgg cttgcttatt gttgcaatag tgataactgt aggtgtttac
    12601 cgtaagttga acgtgaaagg aagtatttct gatggcagat aactcaaact atggatttaa
    12661 acataactta catcaaaaac cttctgaaac aattgtcaga gttgaatggc ttgaaaagca
    12721 tgtcaatagc acctattctg ttacagcaaa aagattaatt gtatcagcta tagttgttgc
    12781 agtcgttgtt cttggatcaa ttgtttactt agtttctgat acgattaaac tgaaatttat
    12841 ttttgttcca acgttattaa tgataattgg atttcttatt ctaattagcc agtatatgtt
    12901 tattgatcca tctagtggtt cattgatttc tggttctatt agactgttca aatcagtagt
    12961 taattcagca agagtatcta tggggaaaaa gaaaccattt cgaggcacag agattttaaa
    13021 agtgaaacaa gatggaacaa tcgtatatcg agatgggagt gtagcattaa tgttcaatct
    13081 agatggggct acatcactaa ccgcttttcc agaagaagtt atggctcagg aatcgaaatc
    13141 aatacaatat caaaatacca gagatcgttc cttagctgaa atcaaaatca cttcatctca
    13201 acctcagaat acggaaagac aaactcgaaa tatgaaaata caaaaacgaa atgcaaggag
    13261 taacaaagca gtctttgaca taatagatca gcaagaacga tttgtatcag aatatattga
    13321 tggtgtgaaa acaacagtaa tccaatatct tatcttgaaa tctcaatcta agatggtatt
    13381 ggatgaaggt gtggaacgtt tagaaaacta tacgagtaga ggactttatt atagtgtcac
    13441 tcagttgtca gctaaacaaa caaaagaaat attatctgat atttatgcac tgaaatagag
    13501 gtgagagatt aaatggaaat tatgggtaga tcacaactat taaatttcaa aaaaaagaga
    13561 aaaactaaaa aagaacaaaa aactagaaat tcaaaggtag tgaataatac ctttgttagt
    13621 gatttaacat ctttagttgg ttatcaattt acaccatctt acattaggac aggtaagcgg
    13681 tggggaacaa ttgtgaaatt agtgaatact tatggaatga atagaaatta tggatttggt
    13741 tggatggtta aagctattcc tgaagtgaga gtagaagggg taaaagccta tctaattgaa
    13801 gcagataagc cagtgtcaca aaaagatcaa caagaaataa tgcacgagaa tgttggggga
    13861 tcaacgaata gttatgaaaa taatttagaa ggaaaaaatt cagatgctga aattagcaaa
    13921 actgttatgc ggattatgga tttgaataca gcatctttac tagacagcat ggcaaataaa
    13981 attattgatt ggaagctaga aatcctatta gtatccgatt ctcctaataa aatcagcgaa
    14041 cagctaatta aactccaacg gttgtatgat gaaaaaatgt atggattaaa gcttatgtca
    14101 tatggaggag atcaacgtga tctgtttgat agacttttaa gtgaaccaat cgggaatgtc
    14161 tatgagtata cgtctatgag ttcggatttt gcaggaaatg atcatgcaat tcgcaaaggt
    14221 ttagatgatg cgaatggatg ggctatcggc actatgtcta ctagctacgc aagtggtgta
    14281 gcttttatgg cactggatga ttcttttaca agtaaagttt tagtggcatc acacaaaaac
    14341 gctacaatta aaggctatga tgaaaaaatc tcagcatcat cactttgggg gcaattgatt
    14401 gctaataatg caatggcaaa tcataaacga acttttcata ttgtcttaaa taaatttgat
    14461 tactttggtg atccaactgg tagaacattt gttgccaaac cttcgttaaa cagtgtaata
    14521 gaaaaggtta atttggctga agaaggtctt aactctttgg agctttttgg taatcaaaag
    14581 gacattggtg atttatatag acggaatctc gataaactaa ctcagatttt ttatttgtta
    14641 gcaggcagaa cattagattc tgcaactaga gttttattga aaaaagaatt aggtaacttt
    14701 tatataagtc agaaactttg ggatattaat tatgaagaac gttcaaaact tttggtgaac
    14761 agtagtgata caatgccagt tcttggggat tttattagct atttgactaa tgctgaaaca
    14821 aaagccaaaa aatctggtac tgaaaaagaa gtcgatcgta ttattgtatt aaaagacaca
    14881 ttgagaacag cattagacag cttctctagg atgtttaatc aaagaacgac actgaaagaa
    14941 atcggtaaaa gtgacaaatt acagttttac tataacttag gtgatctaag agcagaaacc
    15001 aatgttatgg aagctcaatt tttaaatacc tttgatttca tcacatcaca agcaaaaaaa
    15061 gatgatattg ttatgattca cggcttcgat aagttatcta ttgaaaccgt tgggctaatt
    15121 aaaaaaagat taacaatgct tgaagaagat ggaattcgtc ttgcctttct atttgataca
    15181 atcggttcag gagaagacaa tgaatacaaa gaagatattg aaacagctga tatattcaat
    15241 acagatggaa aaatttacca aagttttgaa agtgatttta attacactat cattggaaag
    15301 atgaccagtg gagagttaat gaagtatgaa aaaaaagttg ctcctgctag aaaaggagaa
    15361 gatgatcaaa aactttctgt tgatttgaga cagtatttga caacaggaag aagtaataaa
    15421 tttcagatca ggagaccagt ggatctgact aataattttg ttattggaga gtttattctt
    15481 taagttttat ctttgatttt gagtgtataa ggaaaggata agaaaaaaca tgtatatttg
    15541 agactaaaaa cagtcttaga gaacatggta cgttttgtga aaagagaact atagaaattt
    15601 cataaagtga aaccttactg agtgaaataa ttatagtgga ggttaaataa tgaaaccaaa
    15661 cataaaaatt ttagatcgaa tatttctagg tagagacaca gaagtaatcc taattcaaca
    15721 tgaagaagga tttgaagtta gtataggtat tcagaaactt caaaaacctc attattgtaa
    15781 tcagttgtat aaaaacttta ctgatgaaga aaaagcgaga gtttt
//
LOCUS       DAEGIQ010000062        15639 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000062 DAEGIQ010000000
VERSION     DAEGIQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.063"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            139..705
                     /locus_tag="KMT45_001760"
     CDS             139..705
                     /locus_tag="KMT45_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE7005224.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            850..937
                     /locus_tag="KMT45_001761"
     tRNA            850..937
                     /locus_tag="KMT45_001761"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:882..884,aa:Ser,seq:cga)
     gene            1273..2247
                     /gene="bsh"
                     /locus_tag="KMT45_001762"
     CDS             1273..2247
                     /gene="bsh"
                     /locus_tag="KMT45_001762"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBE7005225.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            2572..4467
                     /gene="asnB"
                     /locus_tag="KMT45_001763"
     CDS             2572..4467
                     /gene="asnB"
                     /locus_tag="KMT45_001763"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBE7005226.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(4662..5108)
                     /locus_tag="KMT45_001764"
     CDS             complement(4662..5108)
                     /locus_tag="KMT45_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBE7005227.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5336..5485
                     /gene="rpmG"
                     /locus_tag="KMT45_001765"
     CDS             5336..5485
                     /gene="rpmG"
                     /locus_tag="KMT45_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBE7005228.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5573..6091
                     /locus_tag="KMT45_001766"
     CDS             5573..6091
                     /locus_tag="KMT45_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBE7005229.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6168..7124)
                     /locus_tag="KMT45_001767"
     CDS             complement(6168..7124)
                     /locus_tag="KMT45_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE7005230.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7290..8096
                     /locus_tag="KMT45_001768"
     CDS             7290..8096
                     /locus_tag="KMT45_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005231.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8093..9082
                     /locus_tag="KMT45_001769"
     CDS             8093..9082
                     /locus_tag="KMT45_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBE7005232.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            9079..10083
                     /locus_tag="KMT45_001770"
     CDS             9079..10083
                     /locus_tag="KMT45_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBE7005233.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            10215..10757
                     /locus_tag="KMT45_001771"
     CDS             10215..10757
                     /locus_tag="KMT45_001771"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBE7005234.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            10777..11475
                     /locus_tag="KMT45_001772"
     CDS             10777..11475
                     /locus_tag="KMT45_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBE7005235.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            11496..11708
                     /locus_tag="KMT45_001773"
     CDS             11496..11708
                     /locus_tag="KMT45_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBE7005236.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            11708..12670
                     /locus_tag="KMT45_001774"
     CDS             11708..12670
                     /locus_tag="KMT45_001774"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBE7005237.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            12688..13083
                     /locus_tag="KMT45_001775"
     CDS             12688..13083
                     /locus_tag="KMT45_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBE7005238.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            13246..13611
                     /locus_tag="KMT45_001776"
     CDS             13246..13611
                     /locus_tag="KMT45_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBE7005239.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            13608..15419
                     /locus_tag="KMT45_001777"
     CDS             13608..15419
                     /locus_tag="KMT45_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBE7005240.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(15481..15627)
                     /locus_tag="KMT45_001778"
     CDS             complement(15481..15627)
                     /locus_tag="KMT45_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBE7005241.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
BASE COUNT         4875 a         2627 c         3275 g         4862 t
ORIGIN      
        1 ttaattttgc aaaaaaaagt taaagaagac tgaaaaaaat ttaaaatata acgttttgag
       61 taaaatgttt gcaaaaaatg ctaaaaatag ggtacaataa aacaacatat actgcagtaa
      121 agaagtgggg gagttttaat gagaagaaaa gtctatacca aggatcaaat tctaaaagct
      181 gcttatgatg ttgttgcaaa ggaagggttt aaaggattta ctgcgagaaa tattgctaaa
      241 aagatgggga tttcgacaca gccgatttat ctggaattca aaaacatgga agatttaaaa
      301 aatacgttgc tggattctat ctttgaacaa ctaaaagaag atgttttccc tattgaacgt
      361 actggtgata aaatcattga tctcggtttg aactatatct actttgcaaa aaagcatcat
      421 agtctttaca tggcattgta cattgatgaa catggggacg gaaagaaaat gcatgatttt
      481 tcatacaatt atttccgtga aatagttaaa gaagatgaaa gatacaatca attatcggat
      541 gaatatgtcg atgctctgca tactggtaca tgggtagcag tgactggctt agcgtcattg
      601 atgtcatcaa acatcatgca tccaacagaa gaacaaatca ttgcactgat caaacggaca
      661 attgactcga ttttagcatt agataatcct gaaataatca gatagtgttc tatcagtatg
      721 aatttgatcg gtaagtccgt tggctgcaaa ctgtcaatgg acttttttgc taaaaaaagc
      781 gatttctgtt atttatctat tagaaatagg cttgacattt cgtgaaaaaa gtttaaaaat
      841 agtacttacg gaaggttggc agagtggtaa tgcaccggac tcgaaatccg gcgaaccggc
      901 taataccggc gcgcaggttc aaatcctgta ccttccttat atcaacgatt agaggttgtg
      961 aagaagctgt cctgcatcaa gtaataagaa cggccaaaca aaaatagctt ttcctatttt
     1021 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaata
     1081 aggggctgtg acaagacttt tgtcacagcc ccttattgtt gtacttttaa cctacaatat
     1141 tttttgattc cccctgagta ctatttttta taaatgctag taaatcaatc ttctttggcc
     1201 ttttttttga taaaaaaaaa tactagatgt aagataaaag tgtaaaaacg atttgtttta
     1261 ggagggaaaa cgatgtgtac cgcaatcact tatgcaacga aagaccatta ttttggaagg
     1321 aattttgatt atgagatgtc ttataatgaa gtagtcacta ttactccaag aaattatcgt
     1381 ttcgatttta gaaaggtaaa aaatttagat aaacattatg caatgatagg aatagcagct
     1441 ggggtatcca actatccact ctattatgag gctacaaatg aaaaagggtt gagtatggca
     1501 ggactgaatt ttcctggcaa tgctgattat aaagaattac aagaaggaaa agataacgtt
     1561 gcaccatttg agtttattcc atggatacta gggcaatgct caaccataga cgaagctaaa
     1621 gaattattgg ctactatcaa tctagtcaat atcgatttta gtgaaaaact acccttgtca
     1681 cctttacact ggttattagc tgataaagaa aaatcgatag taattgaaag tatgaaagat
     1741 ggacttcatt tatatgataa tcctgtcggt gtgttgacaa ataatccgcc atttgattat
     1801 caattattta atctgaacaa ttatcgctcg ctatcaaatg gaacaccaga aaatcatttt
     1861 tcaaaccaga tcagcttgaa tgtttatagt cgtgggatgg gaggattggg cttacctgga
     1921 gatctctctt ctgtttctcg atttgtaaaa gccacattta ctaaaatgaa tgctgcatca
     1981 ggtgattcag aatctgaaag tatcagtcaa ttttttcata tactaggctc agtcgagcaa
     2041 caaaaaggag tatgcgatac tggagaagga aaatacgaat atacgattta ttcttcttgc
     2101 tgtaacgttg ataaagggat ttattattat cgtacgtatg aagacagtca aattactgct
     2161 gtaaatataa ataaagaaga cttggatcgt catgaattgg tcagttatcc agtcataaaa
     2221 gagcaacaga taaactatgt caattaatta atgaatagat ccgtttggta tcatattttc
     2281 tgatacagtc ataattagac acgatcttta ggtcgtgtct ttttttgctg ttttttttgg
     2341 aaatagcatc cactaatatt tttttaacac gaagagaata aggtagcctc aaaaaagaaa
     2401 acatggtaaa ttagatattg ggactttatt ttctgtaaaa acagaaactt tcatcttttg
     2461 ttttcacgtg acgaaattgt aagagagaaa atattcgtcc gctatttatt ctatgataag
     2521 atgcatatga atgaaataaa cagtaatttt atattaggat gtgaaagtta aatgtgcggt
     2581 attgtaggtt ttgttaacga caaggacaac aaaaaaacaa ttattaatac aatgatggat
     2641 cgaatcgttc accgtggacc taatagttca ggagaatata tcgataaaca tgttgcttta
     2701 ggattcagaa gattaagtat tattgactta gagggcggta ctcagcctat ttacaatgaa
     2761 gatcggacaa agatcattat ttttaatggc gaaatctata attaccagcc tttgagagaa
     2821 gaattgattg cagctggcca cgtttttcaa acacatgcag atacagaagt ccttttgcat
     2881 ggttacgaag aatggggaac agaattattg caaaagatcc gcggaatgtt tgcttttgct
     2941 atttgggata atgaaaagaa tgagttattc ggtgcaagag accactttgg gatcaaacca
     3001 tattattatg cagaaatgaa cggaacattt atgtttggtt cagaaatcaa aagttttctg
     3061 cctcatcctg attttaacaa agaattgaac aaagaagcgt tgaaacctta tatgactttc
     3121 caatattctc cattaaacgg cgaaacattc ttcaaagatg tctatcgttt gcctgaaggc
     3181 cattattata cgtataaaga tggaaaacta gacattcagc aatattggga tgcagatttt
     3241 gaaacaaaag aaacgcattc ccgacaagaa tggatcgaaa aaatcgacga aaccgttcaa
     3301 gcttctattg aagcacatac ggtcagtgat gtagaagttg gttctttctt atccagcggg
     3361 gttgattcca gttatgtaac ctctgtattg aaaccagatc attctttctc aatcgggttt
     3421 gatgataaaa cctataatga agcgattgaa gccagaaaac taaccgaact attggactta
     3481 gataatacag cagcagtgat cgacggagac atgtcgttca aagcattccc tttgatccag
     3541 tatcacttag atgaacctga ttcaaatcct tcttgcgtac ctttgtactt tttagcaaat
     3601 cttgcttcac aaagtgtgcg ggtagtccaa tctggagaag gggcggatga actgtttgcg
     3661 ggataccaaa cgtatggatt ccatacgaat tcaaaattca tccgtgtgat tgctcaagga
     3721 ttgaaaaaat taccaaaagg aacacgctat aatttaggac gtaaaatcgg aaaaatgaaa
     3781 aatttccatg gacggatcca tttatatgaa tcacttgctc ctgcaaaaga atattttatc
     3841 ggtcatgctc gagtgttcga agaatcagaa gcatcagaag tgttaacacc aaaatatcaa
     3901 acagcaccat ctgtggatga aatcatgact gttcattacg agaaaacaga aggaatcaaa
     3961 gatgaagtca ataagatgca atatgtcgat cttcatcaat ggatgccaaa agatatcttg
     4021 ttaaaggcag ataaactttc tatggcaagt tctttggaag tccgagtacc tttattggat
     4081 atcgaagtca tgaaattggc tcaacaaata ccaagtaagt ttttattgaa tcaaaacaac
     4141 acaaaagata ttttcagaca agctgcgaac aaacatcttc cggaagaatg gtcgaatcgt
     4201 gtgaaattag gattccctgt gccaatcaaa gcttggttga aagaagaaca cggctacgaa
     4261 caagtcaaag cattattcga agcagatttt gcaaaagaat tttttgatca agaaaaaatc
     4321 atgaagttgc tagatgacca tcacgaagga cgtaaagaag agcaaagaaa aatctggaca
     4381 atcttcagtt tcttgacatg gtataaagtc tactttgtag atgaaacgat tccgcaagca
     4441 gaagcaatcg actatgtgac tgtttgatga aaatcaaata tcaataaaaa agaggttttc
     4501 tcctaccgtt taacactgag tgataagaaa caatttcaaa aaacagctct tcccgtttta
     4561 tagatattat gacttatggt tcggagttgg cagaggaaca ccattgtttc gaacataagg
     4621 gggttgtgac acgattttcg tcacaacccc tttacatatg ttcaataatc gatttctttt
     4681 tcttttgtcc catctttttt ataaatgaac aaagttgatt ctttgttttg tgctacttgc
     4741 tttccttttt cgattgcttc tttttttgta tcaaaatggt tgcttgcctt ttttgcccct
     4801 ttagatttca cgatccattg ttcttcctca tattctacga tcacatctga atcaagcagt
     4861 tttccagcac gaggattcgt atcgtgttca tccgtcttgc ttggattttt ttctttttcg
     4921 aaagttcgtt tttctgattc actagcatct tcataccatt tttttgcttg atcaatggca
     4981 attgggatcg cacggttatc agggtaccct tcatctaata atgcatttgc gatgtcgatt
     5041 gctttttttc ttgtcaattt atctaagttt ttcattgatg caggaaagtc cttcatgttc
     5101 catggcatat tagtatcccc ctttattaaa aaacataatc tatataaaca agtgtaaggg
     5161 tatttataca aaaagaaaaa gattttgttt gagaagtaaa accgttgtat ttatcgtcaa
     5221 gtagttttac ttaacaaaat cgtgaaaaac actagcaatc tagaaaaaat catggtaaga
     5281 taatcaagtc tgagagtcat cttagaggat tcttgtaaaa atgggaggga aaaacatgcg
     5341 cgtaaacatc actttagagt gtacttcttg taaagaacgt aactatctaa caagcaaaaa
     5401 caaacgtaac aatcctgatc gtttggaaaa acaaaaatat tgcccacgcg aacgcaaagt
     5461 tactttgcac cgtgaaacaa aataatgttt ttaaagttct caattccttg ctggtcaaag
     5521 aattgagact ttttttatct caaaacatct aaaatcataa ggagcggata atatgtttat
     5581 ttccatgtgg gcacaagata aaaatggtct gatcggcaaa aatggtttgt taccatggcg
     5641 attgccaaat gatatgcgtt tctttcgcga acatacaatg gatcgaattt tagtgatggg
     5701 aagaaagacc tatgaaggaa tgggggatct ttcacttcct tatcgccata tcatcgtgtt
     5761 gacaacgcaa ccaaattttc gcacgaaaga aaatgcggaa gtaatgcatt cgatcgatga
     5821 acttttgtcg tatagtcaaa cgatctcaga agatatctat gtttcgggtg gtagtcggat
     5881 ttttcaagaa cttcttcctt acactggtcg gatctggcga acattgatcg atagcacgtt
     5941 tgaaggcgat acgtatattg ggaatattga tttttcagac ttcgccctta tagaggagta
     6001 tgaggggata acggatcaag aaaatctata tgcccatcgt tttcaaaaat gggaaagagt
     6061 aaaaaatagg gttgcttcaa aagaagaata aaatctatgc gtctagtttg ttaataagaa
     6121 aaaaagcatc tcacgactaa tcgtgaagat tcttttttta gttaaaatca atttagtttt
     6181 tctttgatat cattgaccat ttcctgactg gcaataggtc cgttatatag ccaactagat
     6241 tcaggtccga attgcagtac atgattgtct gtcactgctt taagattctt ccagcttggt
     6301 tcattgaaca tggcagcatc tttgtcactg ttcacaagga tgatgtagtc agcatctaat
     6361 tcagctagct tttcagagga aacagcagac cagtcagaag tggctttttt ggagacctct
     6421 tccactagat caggaacacc aaattccaga tcgtcgtata cgattcttcc acttgaccga
     6481 tttttgctga ccataaaggc tgagttgttg gtcacccaca acactgcagc tgtcttgttc
     6541 tcgattttat ctgctagttc ttctcgagtt ttcttgatgg atttttgata atcggaaatg
     6601 attttttctg cctctttttc ttttgccaaa gctttgccga catcttttag ttgttcttcc
     6661 catgtgacat ctgttccgtt ttttacaaca taagttggcg cgattttgct atattgttcg
     6721 tatttcccgc cttcgacagt agcggaagaa gaaatcaaca gcaaatcagg ctcaaagctt
     6781 aacactttct cataaggaag gtcataagaa atcgttggga catcctttaa atcatcttgc
     6841 agataatctt gaatggaacc actgccaaca gtccactggg cgataggttt ttcccctagt
     6901 gcaatcagat agtcttctaa gtaactgccg atgatccgtt tgggcgcgtt tggaatctct
     6961 actttatgtc caagtgtatc tgtgactgta tgagtactat tggatgaagt cgtcgaattg
     7021 gcagttgtag ctgtttcttt atctgaagag ggggagcaag cgctcagtac tccagccagc
     7081 agcaatatgc tagataatgt gaatactttt gtttttaatt tcatttcttt cctcctaaca
     7141 taattgagaa tctttctcat tggttattat tcctgatatt tcgaacgctg tcaaccgctt
     7201 catttggcta tagatatgaa agtagcgctt gacgaaatgt tgaaacagcg tcataattga
     7261 gaatgatttt cagcgaaagg atgggtgaaa tgacgagatt aagagcagct gacattgcag
     7321 ccggctacga aaacaaaaaa atcattgaaa acatgtctgt tacgattcct gaagggaaaa
     7381 tcactagttt gattgggcca aatggaagtg gaaaatctac tttattgaaa agttttaccc
     7441 gtatactacc tgtgaaatca ggaaagatca ttgtcaataa ctctgatatc tctacataca
     7501 gttcaaaaga acttgcaaaa aaaattacat tattagcaca aactagtgaa caccctacag
     7561 gtatgacagt agaagaagta gtatcttacg gacgataccc ttatcaaaaa ctgttcagtg
     7621 gcatggacac gcaagatcat gaagcaatcg agtgggcgtt ggatgctaca ggtctagctg
     7681 gatttcgtat gcgggatatc gcaacgctgt ctggtggaca aacccagcgt gtctggatcg
     7741 ctatggcgct tgctcaggaa acagatatct tgatcttgga tgaaccgaca acatttttag
     7801 atccggcaca ccaattagag atcctacagt tattgactga aatcaatcaa atgaaacaaa
     7861 caacgatctt gatgtcgatt catgacatta atcatgcttc acgtttttca gattatttat
     7921 tagcaatgaa aaacggtaag ttgatcacag aaggtacacc tgaagaagtg atcacaaaag
     7981 agtgtatgaa aaaaatattt gagattgatg caacgattgt cacgcttcca agaacaaata
     8041 aaccagtttt tctaacctat gatttgttgc caaaagagca ggaggatcaa ttatgaggac
     8101 tatgaagaaa aatagactcc tgcttttggt ctgttttgtt cttttgatta ttttttttgt
     8161 tgctctttca aatggcgcag cgactgtggg aaaagcagat ttttggcagg cgttgtttga
     8221 ttttgataga tcaaaacaaa gtcaccaaat catacgaaat attcgtttgc ctcgtgtggc
     8281 gggagcattt cttgtaggga gttgttttgc attgagcggt gcattgatgc aaggagtgac
     8341 aagaaatcct ttagcagatt caggcttact aggaatcaac gcgggtgctt cgttggcaat
     8401 ggcccttagt tttgcttttt ggcctaaagc ttctgatttt tctgtgtttc taatgtcatt
     8461 ggcaggttct tttgctgtaa ctttgctcgt atttggtttt ttgcagtttt cgaaaatggg
     8521 catgaatcct ttacagctga ttttagctgg cgtagctatt agttcttttt ttactgctat
     8581 cagccaagca cttacccaat tctttaatct gcagcaggat cttgcttttt ggtttgtagg
     8641 cggagctgcc aatatcacat ggaaacaatt agcagtattg tttcctttat atatacttgc
     8701 tgttttggtt tctttttcat taggaaaagc aatatctttg attcatttga gtgaaacaca
     8761 tgcattagct cttggaagtc atcctaaacg aacgcggttg attgttttct gtctagttgc
     8821 agtgttagca gccattgccg tttcgttagt cggacctgtt tcatttattg ggctgttggt
     8881 tccgccagtt gttcgcggaa tggttggaac ggactatcgt tatgttctac ctgtctcttt
     8941 tttagttggc ggtggattgg ttatgttggc ggattttaca gcgaggatga tcaatccccc
     9001 ttttgaaaca ccttttggat tagtcattgc attgattggc gttccgtttt tactgataca
     9061 agtaaggagg gaacaacggt gaaaaaaata tattggacgt tctatttctt gctttttgta
     9121 cttgtcatcc tgtctatagg tagtttaatg attggtacgc cttttattta cctggatcag
     9181 ctttttgcca cgttcatagg gaaagaatca tcttctttgg cgctgattgt tagagaattt
     9241 cgagttcctc gattactgat cagtttatta gcaggagctt gtcttggcgt ttctggttat
     9301 atcttgcaag gagtaacaag aaatgaattg gcagattcta gtatattggg cattaatgcc
     9361 ggagcaggat ttttagtgat gctttattta ggtttctttt cgcactattc gctgccgttt
     9421 ctattgctag ttgtagcctt tctaggtggt gttttagctg cttgctgtgt ctatcttgcg
     9481 gcctatgatc gaaatggctt tttgggtatg aatcgaatct tattgtccgg tattgccgtt
     9541 aatgccggcc tttctgcatt aacattgcta tgcacgatcc aattatctaa ggaaaattat
     9601 ggatttgtga atgcatggtt agcgggatca atctggggtg cgagctggcc ttatgtttgg
     9661 gcattgcttc catgggcggt tatcttaatt ccgctaggag gactttatgc acagaggatt
     9721 ggattgctat catttggtca agaacgcgcg caaggattag gccttaacgt ggctaaaagt
     9781 caaaaaatcc ttttgttgtt agctgttgct ttggcaagcg gtagtgtagc ggtgacaggt
     9841 agtttatctt ttgtaggact actagctcct catgctgcga agctagtcgt acgtaaagaa
     9901 aataactgga cctttgttct gagcggtttg ttcggttcca tttttctgct tagtgcagat
     9961 atggttgcta gagtgattct accaagtggg gaagtaccta caggaatttt gattgccctt
    10021 tttggtgcgc catattttct gtatgcactt tttatcagac aaaaacacgc gctgaacagt
    10081 tgaaatttat taaaaaagga aggccaatca tttctaggtc ttctttttat tctatttatg
    10141 catttatttg aaagttcgat tgctattttt ctttttttcc tataccattt tatgagagaa
    10201 ggaagggtga gtttttggac caattgaaag taagattacg ccggaaagga atcgaaaagc
    10261 tgaaaaatct ggccaaaagt ggcaagaaaa aggaaaaaga acagcgaatc tataaacagc
    10321 tattcgccag cgagtactgg aagtcagcaa aagtgatagg gacgacttta tcgaatgaat
    10381 ttgaactgga tacacagccg gttatccaac gagcagaaaa agaacaaaag aaaatagttg
    10441 tacctaaaac tttgccgaaa cgtcagatgg cgttttatga aatggatgaa cagtccgttc
    10501 ttgtacgttc gagttttggt gttcttgagc cacaatcaga gaagctctat ttgccggaga
    10561 tgatcgatct cctcattgta ccaggtatta tctacaatag agccggttat cgaatcggtt
    10621 ttggcggtgg ttattacgac cggtatttaa ataattttca taaaaaaaca tgtagccttg
    10681 tttttcgtga acaattgaat gaacaatggc aaccggagag ctttgatcag aagatccaac
    10741 aattgtttat tgactaaaat gaaatgggag gttttcatga attatcaaca gcaaataaaa
    10801 ttgcgtgcat ggttaagacg accgttatgg acctatgctt ttttagggat acagacagcg
    10861 gtcttcctca tgatggaatt atttccgcga cttgagatcc cttattatgc cggaatgtat
    10921 ggtccttatc ttgttcactt caatgaatgg tggcgtttag tgacaccaat cttcatccat
    10981 tttggactga tgcatttcgt aatgaactca ctgatactgt attttatggg gcagcaaatc
    11041 gaggcaatct atggccattg gcgttttttc ttgatttata tgttcagcgg gataatggga
    11101 aatacagcaa gctttgcttt taacgaagcg aatgttctgt ctggtggtgc gagtacttcg
    11161 atattcggtt tgttcggtgc gttgtttatc ttagggtttc attttaaaca taacccagcg
    11221 atccagcagc tagtcagaca ttttctttta tttattgtgt tgacatttgt ttttggtctt
    11281 ctggatacct ctgtagatgt ttggggacat gtcggtgggc taattggcgg attagtgtta
    11341 gggaatgttt tgggtctacc gaaacaacag acaagctatt cgatacacca aaggattctt
    11401 tctacactag tttttgtatt tctttttgta atatgtattt tactgggttt aaaaaaatat
    11461 ggtttacttg tataatatga aggaagtgct taaagatgga gagcttatat gatgtacagc
    11521 agctgttcaa aaaatttggc atctttatct atgtgggtgc acgcatctat gacattgaat
    11581 taatgactat cgaattgcgt aatctgtatg atagccgttt gattgatcat gatacataca
    11641 tgacagcgtg gcgcatatta aaaagagagc accgaattga agaaagccga aagaaaggaa
    11701 atttgtaatg gacaagaaga ttattgggat tgatttaggt ggaacaacag caaaatttgc
    11761 aattttaaca ccagaaggag agattcaaca aaaatggagc atcgatacca atattttgga
    11821 tgatggtaag catatcgttc ctgaaatcat tgaatcaatc aaccatcgtt tgaatttata
    11881 taatatgaaa gccgaagact ttatcggaat cggcatgggt accccaggaa gcgtggacag
    11941 cgaagctgga acggttattg gtgcgtataa cttaaactgg acagaagttc aatttgtgaa
    12001 aaaactgatc gaggcaggaa caggaatcaa attcgctatc gataatgatg cgaatgttgc
    12061 tgctttaggt gaacgatgga aaggtgctgg agaaaacgat ccagacgttg tattcgttac
    12121 tctaggaaca ggtgttggcg gtggtatcgt tgctggcgga aatttgattc acggcgtgag
    12181 cggagcaggc ggagagatcg gccatatcac tgtggatcct gaagggttcg aatgtacttg
    12241 cggtaaacgc ggatgtttag aaactgtgtc tagcgctact ggtgtggtac gtctaggtcg
    12301 ttacttggca gaagaatacg caggagattc aaaactgaaa gcaatgttag acaacggtga
    12361 agaagtaaca agtaaagata tctttgaaat ggctcaagaa gacgatccgt ttgcattgat
    12421 ggttgttgat cgcgtatgct tctacttagg tttagcttgc gccaacttgg ggaatacatt
    12481 gaatccgtca agtattgtgt taggtggagg agtatcggca gcaggagaat ttttacgtag
    12541 ccgtgtagag aaatatttta aagaatttac tttcccgcaa gtaagagaat caacaaaaat
    12601 caaattagca gagcttggta atgaagctgg tgtcattggt gctgcctcat tagccttgaa
    12661 atttgcttaa aaaaacggag gcattacatg gttttatggg taattaatat cattttgcta
    12721 ttgattgtgc ttgcaatcat cttttgggaa gtctatctac gagtaatggc caagcgttca
    12781 gcaacgactt tgacagaaga agaatttcga gaaggaatga gaaaagcaca agtaatcgat
    12841 gttcgcgaaa aagatgtgtt tgatgctggg catattttag gtgcaagaaa catcccttac
    12901 actgttttga aagattcatt aggatctatt cgaaaagacc agcctgtcta tatttacgat
    12961 caaaagaaaa gcttgagcat ccgtacagca aataaattac gtaaagcagg ttatcaagag
    13021 atttatttac ttaagggtgg atatgacgga tggtctggaa aaatcaaaag taaaaaagta
    13081 taaaacagat gattgtatga cagctttaaa aaccgaatca atcattagat tttgaccaag
    13141 atcacatgaa cggcgcaatg gatagttgtg ccgttcattt tttttatttt gtgctattat
    13201 ctacatatat aagctaagcg aaaaggcaga aagaaggaaa caactatgat caaattaaaa
    13261 cgtgtttacc ttgatgctga aaaagaggat ggctatagaa tactcgtgga taggttctgg
    13321 ccacgaggaa tttcaaaaga aaaagcgcag ttagatctgt ggttgaaaga aattgcacct
    13381 agtaatgaag cacgcaaaac ttttgaccat gtaccagaac gatttgcttc atttgcaaag
    13441 aaataccagc aagaattgat tttcgaagaa gaaaagaagc aagctatttt taaactcagt
    13501 caacttatga aagaacatcc agtcattact tttgtttatg gtgcaaaaaa tgaacatcag
    13561 aaccatgctg ttttattgaa aaaatttatc gaaatggagg catgcaaatg aaaataggga
    13621 tcattggtgc cggacctaga ggattaagta tagcagaaag gattctccat aataatcagg
    13681 acagacattc tcttgaactt tttcttttcg atcccaatgg tcctggaggt cgagtttggc
    13741 ggttgaatca gccaccggag ttattaatga attctgtttc tcaacaagtg acgcttttta
    13801 cagatgaaac attgtccagt ggaggattgg taacacaggg acccaacttg tttcaatgga
    13861 gtaaaggacc tgctacagat tatatcgaac agacggtaac caaaaataag caactaatta
    13921 tagaagaaat caatcgtctg gaaaaagatc agcaatctac tcgatgttta tacggactct
    13981 atcaacgctg gtttttctca aagttgcaag aagaatttcc agcttctatt cagtttgttt
    14041 attctttagt aaaaaaagtc agcaaaagaa aagatggtta tcttttacag acagatcagc
    14101 aaagctattc tatcgatcag cttgttttag caagcggtca ctgggaaaat gatgagacag
    14161 aagaagaaag agtgtgggta gactacgcaa agaaaaatga tttactgtat cagcttcctt
    14221 ctaatcctgc agatgtcgat ctttcgaaga taccgcctaa agaaaatgtc tttttaagag
    14281 gtctaggctt aagttttttt gattatgtcg gattgtttac agtatcaaga gaagggcgct
    14341 tcgagaacga aaaaggaaaa cttgtctatc atccatcagg caaggaaccg attgtataca
    14401 gtggatcaag aagggggctc ccttattacc ctcgaggaag aaaccaaaag caaggcggat
    14461 caatggcatg gccacggtta ctaacaaaag aaaacctgga gcaatggcat cagtcgggat
    14521 ttttgactgg cgaaacattt tttgactatc tgaaaaaaga cgttgagttg ttttattaca
    14581 agaaaatcat cgaagaatat catttgccga ttccaaagaa aaattttgag catgattttc
    14641 tctccctctc ttcagaggag tgtctcggaa agcatccagc actaatccct tatagatggg
    14701 attggtcgta tttagaaatt cctcttacct atcaggatga gtcctttgca gaagctagcc
    14761 gagcatttat tgaagggcag ataaaagaag cggaaaaagg caattgcaca ggagcatttg
    14821 cttcaacatt tgatgcatta aaagactggc gtgatccaat ccggcaggct attgaatggc
    14881 acgttttttc tgcaaaggaa tacaaagaaa tactatgggg atggtttact catctaaata
    14941 gctttttaac gatcggtccg ccagtcattc ggacgaaaga attagcagca ctgatagatg
    15001 cgggtatttt tcatttgttc gaaccgccat ttgatattca gcaaaagcac aaccgctatg
    15061 ttatttctct acaagataaa gaaatcagca gtcgctattt gattgaagct cgattgccac
    15121 aaacaagctt ttctcgttca agtaatcctg tcataaaaaa tttgaaggaa aacaaacttg
    15181 ttcgcccttt ttcattttca gataatacag gaagttatca ttcagaagct atagatgttg
    15241 agcgaggtac gaatcgcgtg attgatgctt caggggaaat ccagtctgac ttttattgct
    15301 atggaatacc tgttgaaggt gtggattggc taactgcagc agtagctaga ccttatacag
    15361 atccatggaa tctgagacag gcagataaaa tagcacaact tatattgact gacaaataaa
    15421 aatacgcatg atgacttatc tgttctttca caagtcttca tgcgtgtttt taaataatta
    15481 ttaacgagaa acgcgtttgc gcattgcttt tttacggttt tcttcgatca ttgcttcttt
    15541 ttcgtcaatt ggatcgatca ctgttttttt cacagaagcg accaaaccag caacggcagc
    15601 ggctgttgcc agacttccta ctaatattcc agatacaaa
//
LOCUS       DAEGIQ010000063        14844 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000063 DAEGIQ010000000
VERSION     DAEGIQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.064"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(142..828)
                     /locus_tag="KMT45_001779"
     CDS             complement(142..828)
                     /locus_tag="KMT45_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7005242.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QVKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(964..1158)
                     /locus_tag="KMT45_001780"
     CDS             complement(964..1158)
                     /locus_tag="KMT45_001780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005243.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1208..1648)
                     /locus_tag="KMT45_001781"
     CDS             complement(1208..1648)
                     /locus_tag="KMT45_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBE7005244.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(1652..2497)
                     /locus_tag="KMT45_001782"
     CDS             complement(1652..2497)
                     /locus_tag="KMT45_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBE7005245.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2646..4064
                     /gene="pepV"
                     /locus_tag="KMT45_001783"
     CDS             2646..4064
                     /gene="pepV"
                     /locus_tag="KMT45_001783"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBE7005246.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4121..5068)
                     /locus_tag="KMT45_001784"
     CDS             complement(4121..5068)
                     /locus_tag="KMT45_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBE7005247.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(5291..5641)
                     /locus_tag="KMT45_001785"
     CDS             complement(5291..5641)
                     /locus_tag="KMT45_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBE7005248.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5841..6920
                     /gene="pepA"
                     /locus_tag="KMT45_001786"
     CDS             5841..6920
                     /gene="pepA"
                     /locus_tag="KMT45_001786"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBE7005249.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7064..7384
                     /locus_tag="KMT45_001787"
     CDS             7064..7384
                     /locus_tag="KMT45_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBE7005250.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            7406..7870
                     /locus_tag="KMT45_001788"
     CDS             7406..7870
                     /locus_tag="KMT45_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBE7005251.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            8075..8680
                     /locus_tag="KMT45_001789"
     CDS             8075..8680
                     /locus_tag="KMT45_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBE7005252.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(8719..10008)
                     /locus_tag="KMT45_001790"
     CDS             complement(8719..10008)
                     /locus_tag="KMT45_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBE7005253.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            10436..10915
                     /gene="rlmH"
                     /locus_tag="KMT45_001791"
     CDS             10436..10915
                     /gene="rlmH"
                     /locus_tag="KMT45_001791"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBE7005254.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            11268..11582
                     /locus_tag="KMT45_001792"
     CDS             11268..11582
                     /locus_tag="KMT45_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBE7005255.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            11598..11984
                     /locus_tag="KMT45_001793"
     CDS             11598..11984
                     /locus_tag="KMT45_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBE7005256.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            12013..13398
                     /locus_tag="KMT45_001794"
     CDS             12013..13398
                     /locus_tag="KMT45_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBE7005257.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            13401..13554
                     /locus_tag="KMT45_001795"
                     /pseudo
     CDS             13401..13554
                     /locus_tag="KMT45_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            13577..14782
                     /locus_tag="KMT45_001796"
     CDS             13577..14782
                     /locus_tag="KMT45_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBE7005258.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         4717 a         2794 c         2988 g         4345 t
ORIGIN      
        1 cagctttttc acaacctctc aataaacggt gtgcaatcag ctgaccctcg gtattagctc
       61 aaaaaaaacg gcaaaatatg aggaactatt ttgccgtttc tttctctaat actcaggtca
      121 aaacgctgat tccaaaacct cttatttgat gggtctgtcc tgaatatctt caaaataatc
      181 ggggtaatta tgaatcacgt attcttgctc ctcgataatc atatgtaaat gcatcgtgca
      241 aagaaatccg acttcatcaa agttatgatc aatcattgat tgaagcattg tctgatgttc
      301 atctaatact cgaccccagt ctaattctga aatcgtcaaa cgtaaccatc tgaaacggtt
      361 caagtgcgta ctataacttt ctagccaatc ccaaatttct cgttttccag caatttcata
      421 acaagttcgg tgaaaatcag tatccaaatc aaaaaaagct tttttatttt gagcgattac
      481 tgcctgatcg gtttgttcta gtattttttc tagtttaact tgatcttgct tggtcatttt
      541 cgcactgcat tcttgcagga tcggtttttc caacttttcc cgcgtataac gggctaatga
      601 agcagatcgg atatcgattt ttgaaacata agtgccggac tgtggaatgg tttttacaag
      661 ctcttgattg cgcagttgga tcagtgcttc tcgaatcggt gtacgcccga tagctagttg
      721 ttcttctaac actttatcgg agattttact tccaggtgct aactcggaat aaatgatttg
      781 tttacgtata gattcataag cttgctgttg caaattattg ataaccattt aaccttcctt
      841 tctatgcaga tataatctat tataacataa atcgcttaca aatcgtgtgg gcataaaaac
      901 acagtctgca aaaaaagccc tgataggcat gcctattaga gcctcgaaaa gaaaaatatt
      961 gaatcaatct ggtcgtacct cttggatcgg tttgacacga ccgccgattc ttttagcaaa
     1021 agcttcggat tcttttttgt ctttgaaaat gagcagattg tctaagtttt tttcccgagt
     1081 gcgacagcca gttttatcga tgtaaccgtg cattacttta acgacataca tggggatcac
     1141 ctgctttttt cttttcatta tacgaactac tcgacacaat acaagaaaaa tgcttagacg
     1201 gtctttatta agaatttaca tcatccaaag aagcgtacgc ttggaataac tgatctaact
     1261 ccggattgac tttgctcagc ttcactaatc gttgcgaatc agaaagtaaa aaacagtgtt
     1321 tactactgcc agtagttttt aattttttgt cttcagttcc cacaatttcg tagctaaaat
     1381 ccaaccgtgt ccccgtatac ttttggatag ttggatggat gctcactttt tctccgtaat
     1441 ggatcatttc tttatagctg caatttactt ccagaacggg gatgatgatc ccagattctt
     1501 cgattttttt atagggaaac cctagatgta ccaataaggc tacccgagcc tcttcaaacc
     1561 agcggatata attgctgtga tgaataatcc ccatttgatc tgtttcataa taaaaaggtt
     1621 ctcgcacata acctgtaaat ttctccatga ctcatgcctc gttttccatt gattttttac
     1681 aaaaataatt catccatttc ggaagtgctt cgctgatttg tgtcggcaaa acaatatatt
     1741 gttcttttgc cagctggtct ccaatgaaac tatgaagata aatagctgct gcaatcgttt
     1801 ggtcattttg aggaaactgg gctaaaaatc ctgtgatcat cccagctaaa gtgtcaccca
     1861 tgccgcccgt tgccatagca gcagaaccta aaggattggt gtagaagtgc tggtgaccat
     1921 aaatttttgt ctcatgactt ttcaaaacga gcacagcacc gatttcctgt tgcttctttt
     1981 tgttttcttt atcccgttgt tcttgtatcg gtagagaact caaccgttgc cactccattt
     2041 gatgaggtgt aagtacagtt tgttcgggat agggtagcaa aagatgatta gcagcaagta
     2101 aacttaaagc agagccatca atcaccagcc attgttcttt tgattggttt tctagtactt
     2161 gtttgaaacg ttccaaactc ttagaagaag tacccaatcc gggaccaatc aagagtacat
     2221 ctgcttgctt tattacggaa agaaactggt cagtatccgt ccaaactaca atcattgctt
     2281 ctggcagatg actgtgaaga gcactccgat ttttttcatc agttactaca gtcgtgagac
     2341 ccgctccgct gtgtacggca gctaaagcac tcataatgat tgctccgcca tattgttcat
     2401 tgccgccaat caaaacgata cgtccaaatg ttcctttatg tgaaattttt ggacgaggtg
     2461 tgatgactct tttcactagt tgttcgttta tcagcatcta aaaacctcct tttactcatt
     2521 atagcgtata ttttttttag agaaaaacag ataagaaaag gaattgtcag attttttgaa
     2581 ctattttttt ctgtaatttg tggtatcatg aacacgaaga cttaaataag tggaggatta
     2641 actttatgac aattgattgg caaaaagaag tagaagcacg taaagaagac ttgcttgaag
     2701 acttgaaaaa tcttttacgt gtcaacagcg agcgcgatga ctcaaaagta acaccagatg
     2761 caccatttgg acctggacct cgcgacgcat tgaaacatat gttagcttat ggcgaacgtg
     2821 atggatttaa agtaaaaaat gtcgataact atgcagggca tattgatctt ggcgaaggag
     2881 acgaaacgct agggatattt ggacacatgg atgtcgttcc tgcaggagat ggctgggaca
     2941 cagatcctta tgaaccagta atcaaggatg gaaagatctt tgcacgcgga tcaagcgatg
     3001 acaaaggtcc aagtatggca gcttactatg cgatgaagat cattaaagaa cttgacttga
     3061 aactatcaaa aaaagttcgc ttcgtagtcg gcagcgatga agaaagcggc tggggcgata
     3121 tggcttatta tttcgaacat gaagaagaac cagattttgg tttctcacca gatgctgaat
     3181 tcccaatcat caatggtgaa aaaggaaatg tttctttagc tttacgcttc aaaggcgaca
     3241 atgcaggaga ttatgtcttg aaatcttttg tttccggact tcgcgaaaat atggtgccag
     3301 gaacagcaac tgcagcactt caagtaccaa gtgcagatgc agctatcgcg atggaagaag
     3361 cattttacca attcatcgaa gcaaatccag taagcggaac gattgaagcg gacaatacgt
     3421 atgtaaaaat cgaattagtt ggtaaaggcg cacatggtgc aagcccacaa tctggtatca
     3481 atgcaggttc tttcttggca ttgttcttag ataactatga attcctagga tctgctaaac
     3541 aatttatcca tgtagcagct gcttatgtcc atgaagattt ttatggagaa aaactaggag
     3601 tcgcttatga agatgaaaaa atgggcaaat tgacaatgaa tgctggacta tttgcttttg
     3661 aagaaaatgg tacagaggaa gcgaacttca tcaatatgaa tttccgtttt ccaaaaggtg
     3721 taacagtaga cggattgcaa agtgacatcg aacaaacagt ggggcaagaa ggcgcaacag
     3781 ttactcgagg tgctcgcgta atggaaccac attacgtacc aatggatgat ccattggtgg
     3841 ctacattatt gcaagtctat gaagaccaca ctggagaaaa aggctacgaa caaatcatcg
     3901 gtggcggaac ttacggacgt ttgttgaaac gtggcgttgc ttatggagcg atgttcccag
     3961 gatatactga tacaatgcat caagcaaacg aatttatgag tcttgatgac ttgttcagag
     4021 caacagcaat ctatgcagat gccatctatc gtttggcgaa ataattaatt aaaaagagga
     4081 tcaagaattg caaattattt ttgcagtctt gatccttttt ttactttcta ctgtggtatt
     4141 catgataaac atgatataga ttcaattcat attctttctt agcgttcgtg acgactgtat
     4201 ccaaaatcaa ccctgttact aatgaaagaa gtgctaaagt catcaatcca gtacttaaaa
     4261 tcgcagaagg gatgcgtgta ataaagcttg tcagcatata ttcgcgaatc acaggaatcc
     4321 ccgtgaccaa tccaaataaa aagaagaaga tggtccaaat cccaaagaaa agcaaaggct
     4381 tataatcttt aaacatttta ataatcatca tgatcacttt gaatccgtca gagaatgtat
     4441 tcaattttga ttcgctgcct tcaggacgat cacgataatc gattgggatt tcaacgaact
     4501 tgaatttttt atctaaagca tggatcgtca attccgtttc gatttgaaaa ccagaactca
     4561 taataggaaa acttttgaca aagatacgat tgaatcctcg atagccggtc atgacatcaa
     4621 gaatttttgt tttatacatt ctagtaattg tatttctaac aaggttattt ccaaaatcgt
     4681 ggaaaggtct cttattctca tcaaagtagg ttccatttga taggcgatca ccaatgacca
     4741 tatctgcttc accagaacgt aatttctcga gcaaaccatg tacagcttct gcaggataag
     4801 tatcatcgcc atctaccatt aaataatagt cggcatcgat atcaaagaac atttggcgga
     4861 tcacatttcc ttttccttga cgaggttctt tttttacgat tgctccacct tgaacggcca
     4921 attcgtatgt tcgatcggtc gaattattat catagacata aatatctgct tctggtaatt
     4981 ctcgtttaaa gtctgcaatg actgttgaaa ttgttgcttc ctcattgtaa caaggaatca
     5041 gaacagcaat tttttcttgt tgttccattt ttcactctcc ttaactatag aaaacaaaag
     5101 ctaaattaaa aatacctcat aatcaaagga aatgctagtt tttcccgcta aatatcattt
     5161 atttaaaaag aaaatactgg aatatatgtt tggaaagctc tatctaaaaa accacccact
     5221 acaaaagaag gacatgatac ctctcttttg aatagtgggt gcgtattcgt tctctcactt
     5281 ttttgacgta ttaatctgtt gcaccttttt ttcttgaaat ttctaatacg gttcctgtat
     5341 gagcatctgc taaaaattca taataaacag tttcgccatc ttctattcga gaaagacctc
     5401 cccgataagc actcgaccga acggcgaatt tcctaatagg ttccttttca aaagagatcc
     5461 aagaaccttc gattgcacct tcttttagaa aagatgactt gatattttca agtacttggt
     5521 ctgctgacaa tacttttttg cgatgatacc aaatcgttga agctaccccg ctcaataatc
     5581 ctagaccgat tccaactgct aacccgcctt taaaatagct tagttcgctt tcttcataca
     5641 tgtggacata cctccttctt ctcacttgtt actccttgtg cttattttac cacactctca
     5701 tattttcttt gaaataaata accgctacca gaaaaaataa gctataataa atacgatcgg
     5761 tctgcagaaa aaatcatatt ttatccgcag atcaatataa gtctgagagg agtttagtct
     5821 gaaatttaga cgaatagcat atggaagaaa aaacatttca acgaatcaaa gagttaacag
     5881 aattacaagg aacaagcggt tttgaacaag atgttcgcgc ttatatggaa agtcatatgg
     5941 aaccgctggt agatgagtta cagttagatg gtttgggcgg catttttggt ctgcgccatc
     6001 atgaagaagc ggatgcaccc cgtgtaatgg tcgcagcaca tatggatgaa gtcggattca
     6061 tgctgacaca aatccaagaa aacgggcttt ttaaagtcgt acctctaggc ggctggaatc
     6121 cttacgttgt ttctgctcag cgttttacac ttaaaacctc tactggtaag aattatccat
     6181 gtatttcttc ttccgttccc ccacatttat tgcgaggaac aagcggacaa aaatcagtag
     6241 aagtaaccga tattttgttc gatgctgggt ttgaatcaag agaagaggca atgagttacg
     6301 gtgtccttcc aggagacaca atcgttcctt acgctgaaac gatcaaaaca gccaacggaa
     6361 agaatattat cagtaaatca tgggacaacc gctatggctg tacaatggta ttagaagctt
     6421 tagaagcgtt gcaaaatgaa acattaggtc atacattgat tgcaggcgcg aatgtccagg
     6481 aagaagtagg cttgcgtggt tctaaagctt ctgtcaataa attcaagcca gatctgttct
     6541 ttgcagtaga ctgctctgca gcagatgata cagtgacgaa aaatggcaca ttcggacatt
     6601 taggagaagg tacattgatg cgtatccaag atccaggttt gatcatgctt cctcgtttgc
     6661 gcgagtactt gctggatatc gctgaaacaa acaatatccc ttatcaatac tttgtttcaa
     6721 aaggcggtac agatgcaggt gctgctcata cacaaaacga aggaatccca agtacagtga
     6781 ttggcgtagt gggacgttat atccatacgc atcaaacgat gttcagtatt cgcgattttg
     6841 aagctgcacg agaaatgttg attcaaacac tgaaaggttt ggataaatca acagtaaata
     6901 cgatcgtata cggaaaataa tgcgatagtg aaactataaa atctggcaaa agattgcttt
     6961 ttgccagatt ttttttataa aaagaggatc aatgtttgtt agttcgaaag aaaggtgtat
     7021 aatttaattg aaaaatacat aaacagaaag tagaggacaa agaatgatta tcccaaatag
     7081 tttagaagaa ttagcaggct acgtagaaac tggaaagagt gtatttttct ttactgccgg
     7141 ctggtgcgga gactgccgtt ttatcaaacc gcaaatgcct gaaattgaaa atgacttttc
     7201 tgattggcaa tttattgaag tagaccgtga caagtacata gatgtagccg ctgaatggaa
     7261 catttttggt atcccaagtt ttgtcgtgat ccaagacgga aaagaattag gtcgtctagt
     7321 gaataaagat cgtaaaacaa aacaggaaat cgaatcgttc ctagaaagtg taacaaaagg
     7381 ctgacatctg ttaggagggg tcaagatgaa tcaagaatat aagaatattt tagtaggtat
     7441 cgacggtagt gaacaagcac ttgctgcttt caaaaaagcg gtagaagtgg ctcgccgtaa
     7501 taatgggaaa gtatatgtag caaacgtaat cgatcagcaa ttttataact ttatgggcta
     7561 tgcacctatt gatcaaaatc tagtcgatca gcaaactgaa acagccaagg aaatgatcga
     7621 agaatgtaaa gaatatggga aatctgttga ttacacaaat atagaaggaa ttattgcata
     7681 tggttctgtt cgtgaatcca tggcagtcaa attgccaaaa aaatatgaga tcgatctaat
     7741 catggttggg cagtcaggtc taaatgcagt cgaacgcttt atgacaggaa gtgtagccag
     7801 ctacgtgata cgtcattcac cttgtgatgt cttgatcgtt tctgacgaag aagaacagcc
     7861 aagtaattaa gcaagtggaa cttgtacgac ctttttaatc aattaactaa aacagtaaag
     7921 ggactgtgac aaaagtcttg tcacagtccc ttgttacgaa taaatggtgt tccagaagag
     7981 gcttcttcgg aaataaagca tgaaaaaaat ttcatgcagt gaatgcaatt ttttgttaag
     8041 ataaatctca gcaaaggata atggaggaaa gaacatgatt tttgcttata acaaagcaca
     8101 cgtcggagac accttgatgg ttattatagc cgacgataaa ggaacagaaa acactgttga
     8161 acgcaaatgg aatgttgctc ggatcaaaga tgaacaaggt caagttgttg cttggaactt
     8221 tttccatatc tccgatcatc taacaatcga agggaacggc caagtaagct taacagatga
     8281 ggaattagcg aaattgaatc gattgatcaa agaagcagga ttcgaagaac agcttgaagc
     8341 tgatcatgaa ccgaagatcg ttgtcgggta cgtaaaaaca tgcgtcccac atcctgattc
     8401 tgaccattta tcgattactg agacagaagt agacaatggt acagtattgc aaatcgtttg
     8461 cggtgcaccc aatatcgaag ctggacaaaa agttgtcgtt gcaaaaccag gagctatgat
     8521 gccagatgga atgatgatct ggccaggaac tttgcgaggg gtagatagct atggaatgat
     8581 ctgttccgct cgtgaattgc atttgccaaa tgctccagca aaaaaaggaa tcttagtatt
     8641 gcctgaggat gccgtagtcg gagaagcatt tccaaaataa agaggttgtg acaagtcttt
     8701 gtcacaacct cttttttatt aattttcact attttgattt tgttttaatg ccgattgatc
     8761 gatcgtcaat tcaacagtag ttgttttttc tttcgagcct tcatagtaag tgattttgac
     8821 agaatctccc acttttttct tatacaaagc agattgaagt tctacaccag aagaaacttc
     8881 tttgtcatcg atcttagtaa tgacgtcgta ttgtttcaat ccagcttttt cagccggtgt
     8941 agctgtttgg acatttgtca cgatgacacc gttagttaca gatgaaggaa tcttcaagat
     9001 ttgttcttgt tgctgagtag aaacggcaga taagtcgacc attgtgatac ctagagctgg
     9061 acgtgtcact tttccgtctt tttccagttg attgatgata ttcactacat cgttacttgg
     9121 aatagcaaat cccattcctt ctacgcttac attagaagta gattcagaag tacttgcgat
     9181 cttacttgag ttgataccga taacttgtcc ttcgatatta actaaaggac caccagaatt
     9241 tccagggttg attgctgcat ctgtttggat cgcattgata ttgactgttt cgttcgattc
     9301 attcgtactt gtcacttgtc ggttcaagga agaaatgatt cctgaagtga cagaatttgc
     9361 atattcagat cctagtgggg aaccaatcgc aatcgcaggc tcaccaacct tcagagcact
     9421 tgagtcacca aaagaggcaa ctgtttcgac tttgtctgaa ttgatcttca acaccgctaa
     9481 atctgaataa gcatctgttc caactaattc agcttttacc ttcgtaccgt ctttcatcaa
     9541 gacttcaaga ccttgctggc catctactac gtgattattc gtgactacat aagcagtatt
     9601 cccgtctttt ttgtagatca cgccgcttcc ttcactataa gcttccaaat tactatcatc
     9661 tgagctgcta ctttcttctt gctgcccgaa tagtccgcca aaaccgcttg attggttttg
     9721 actttgtaag ttgataacag aaacgacagc gtcttgaact ttatctaccg ctttggtgat
     9781 atcagaatct acattgactt tgacattttc cacaactgtt tcaccggcag aattctgatt
     9841 gccgcttgta gcagtagaac tattgttgcc agtgcccatc gctgcataaa aaatgccagc
     9901 tgtcagtagt cctccaacga tccctccaac aagccccaga cccaacttac gccagaggct
     9961 attattttta ttttttttca tctttggtgt cacatttttt ctatccatgt gttcctcctc
    10021 caattacatt catttatcta ctgttagtat agtcttattt gttgacttta gtctactttg
    10081 agactttgaa ctttttatga aaaactttag gaaaaatcat tgttttcaga tgaaaatatt
    10141 tgattttctt tttccacaga tattgtgtaa aactacgcgg ataggattgg aaaaataagg
    10201 gataaaacat atccacagcc tgtgtataaa gtggataaat tcttataaaa cgtatgtttg
    10261 cctaatgaat gtgatgaaat aaggtaaaac aacgctgttt tctgtggata aacctgtgga
    10321 tgttgtgcat aactgttggg gaaatctttt taacaaaaat agtcaaaata aacatataga
    10381 cgaaaaaatt gattataata aacaaagtga gcgtgaagat caaaggagag aaaacatgaa
    10441 tatcaaaatt atttcagtag gaaaattaaa agaaaaatac ttgatccaag gtatcaatga
    10501 gtatgtcaaa cggctgaatg cctatgcgaa aatcgaattg atcgaagttc ctgacgaaaa
    10561 agccccggag aatttaagcg aagcgcaaat gagacaagta aaagaaaaag aaggagaacg
    10621 gatcttagcg aaaatcaaag aacaagaata cgtctatggc ttagcaattg aagggaaaaa
    10681 tcccacaagc gaatcttttg ctaaacaaat cgaccaatta ggtatccaag gaaaaagcca
    10741 ccttgttttt gttatcggcg gttctttagg actcagcgaa gccgtcatga aaagaagcaa
    10801 tgctcagatc tcttttggca aaatgacgta tccacatcaa ttgatgcgcc tgatcctcgt
    10861 cgagcaaatc taccgtgcct tccggatcaa cgcgggggct ccataccaca aataatgata
    10921 ttttttaaga gaaaaatagc ataaaaatct agttatccgc ataaaaactg gacttatcac
    10981 actttatcaa ggtcaaaacc actcaattta ctactaattt actacttatg aatgagcttt
    11041 gatacgacga tttatccttg aaaagtgaag atataaagat acttccaata aaatttgaat
    11101 atttaatagg tagacacttc aaaaaatgag gtgtctattt ttttacccga ttttgaaagg
    11161 aagtgaactt atgaaaacaa aaaatcaaga atcaaaaggt cgttccccac tctttaagac
    11221 catcaaacat tcattcagcc aataaaaaag aaaggatagg taaaaatatg gaacttaaat
    11281 ttgtgattcc caacatggaa aaaacattcg gcaatttaga atttgctggc gaggataaag
    11341 tcgttcagcg aagaatcaac ggacggctaa ctgtcttatc aagaagctat aatctctatt
    11401 ctgatgttca aagagcagat gatattgtgg tggtgcttcc tgctgaagct ggcgaaaaac
    11461 atttcggctt tgaggaacgt gtgaagttag tcaatccacg tattaccgca gagggctaca
    11521 aaatcggcac tcgtggtttt acaaattacc ttttacatgc tgacgacatg ataaaagaat
    11581 aaagaaagag aggaaaaatg atgagattag caaatggcat tgtattagat aaagacacga
    11641 cttttggaga attgaaattc tctgctctac gtcgtgaagt gagaatccaa aatgaagacg
    11701 ggtcggtttc agatgaaatc aaggaacgta cctatgactt aaaatccaaa ggacaaggac
    11761 gcatgattca agtaagtatt cctgccagcg tgcctttgaa agagtttgat tataacgcac
    11821 gggtggaact tatcaatccc attgcggaca ccgttgctac tgccacctat caaggagcag
    11881 atgttgactg gtatatcaag gcagacgata ttgtgctgac aaaggattct agttcattca
    11941 aagctcaacc acaagcaaag aaagaaccga cacaagacaa atagtcgcta ggtagaaagg
    12001 agactttttc gcatgaaaca gcgtggtaaa aggattcgcc catctggtaa agatttagtc
    12061 tttcatttta cgatagcgtc actcctgcct gttttcctgc tggttgtcgg actgtttcat
    12121 gtgaagacaa tccagcagat caactggcag gattttaacc tatcacaagc agataagatt
    12181 gacattccct atttaattat cagtttcagt gtcgcaattc ttatctgctt gctggtagcg
    12241 tttgtattca aacgggttcg ctatgatacg gttaaacaac tttaccaccg tcaaaaactg
    12301 gcaaagatga tacttgaaaa caagtggtat gaatctgaac aggtcaaaac agagggtttc
    12361 tttaaagata gtgctggtcg tacaaaggaa aagataacct acttccctaa aatgtattat
    12421 cgacttaaaa atggcttgat acagatacgg gtggaaatca cgctgggaaa atatcaagac
    12481 caactcttac acttggaaaa gaaattagag agtggcttgt actgtgagct gacggataaa
    12541 gagttaaagg attcctatgt ggaatatact ttgctctatg acaccatagc cagtcgtatt
    12601 tctattgatg aagtagaagc taaagatggt aaacttcgct taatgaaaaa cgtatggtgg
    12661 gaatatgata agctccctca tatgttgatt gctggtggta caggtggcgg taaaacttac
    12721 tttatactga cactgattga agccttgctt catacagatt caaaactgta tattcttgac
    12781 ccgaaaaatg ctgatcttgc ggacttaggt tctgtgatgg caaatgtcta ctatagaaaa
    12841 gaagacttgc tttcttgcat tgaaacattc tatgaagaaa tgatgaaacg tagtgaggaa
    12901 atgaagcaga tgaagaacta taagactggc aaaaattatg cttacttagg tctcccggca
    12961 cacttcttaa tctttgatga atacgtcgct ttcatggaaa tgctgggaac aaaagaaaac
    13021 accgcagtta tgaataagct gaaacagatt gtcatgttag gtcgtcaagc tggcttcttt
    13081 ctaatactgg cttgtcaacg tccagacgca aaatatttag gcgacggaat ccgtgatcag
    13141 tttaatttca gagtggcttt aggtcgtatg tctgaaatgg gctatggcat gatgtttggc
    13201 agtgacgtac aaaaggattt cttcttaaag cgaatcaaag gtcgtggcta tgttgatgta
    13261 ggaacaagtg tcatatcaga gttttatact ccccttgtac caaaaggata tgatttcttg
    13321 gaggaaatta aaaagttatc caacagcaga cagtccacgc aggcgacgtg cgaagcggaa
    13381 gtcgcaggtg tggactgatc ttgctggctg gtgtggcaat agccacgcca gcacttaacc
    13441 cctcggtatc taacaggggg gtacaaatcg acaggaaaca gtcaaaaaaa cattagaaaa
    13501 tcctttggtt acaagggatt tacaaaattt cagcgtatgt caaatgggct ttaaaagttg
    13561 acatacgcct ttttgattgg agggattttt actgaatgaa caaacttggt tacagcattt
    13621 aaaagaaaaa cgcttggctt atggactatc tcaaaaccgt ttagctgttg cgactggtat
    13681 tacaaggcag tatctaagcg atattgaaac aggaaaagtc aagccatcag aggatttaca
    13741 gcagtccctt tgggaagctc tggaacgctt caatcccgac gctccccttg aaatgctgtt
    13801 tgattatgta agaattcgct ttccgacaac agacgtacag caggtggtcg aaaacatctt
    13861 acaactgaaa ctgtcctatt ttcttcatga ggactatggt ttctattctt attcagagca
    13921 ttatgcttta ggcgacatat tcgtcctttg ctcccatgaa ctggacaaag gagttctggt
    13981 ggaattgaaa ggtcgtgggt gcagacaatt tgaaagctat cttctggcac aacaaagaag
    14041 ctggtatgag ttctttatgg acgttttggt ggctggcggt gtgatgaaac gccttgacct
    14101 tgccattaac gataagacag ggattttaaa tatccctgta ctcactgaaa agtgccaaca
    14161 ggaagaatgt atctccgtct tccgcagttt taaaagctat cgcagtggcg aactggtacg
    14221 caaagaggaa aaggaatgta tgggaaacac cctctatatc ggttcattac aaagtgaagt
    14281 ttatttctgt atctatgaaa aggactacga gcagtacaag aaaaatgata ttcccattga
    14341 agacgcagaa gtaaaaaacc gttttgagat tcgattgaaa aatgagcgtg cctattatgc
    14401 agtccgtgat ttactcgtct atgacaatcc agagcatacc gcctttaaaa ttatcaatcg
    14461 gtatatccgt tttgtagata aagacgattc caaacctcgt tctgattgga aactgaatga
    14521 agaatgggct tggtttattg ggaacaatcg tgaacgatta aaactaacca caaaaccaga
    14581 gccttactcc ttccaaagga cgctgaactg gctatctcat caagttgccc cgaccttaaa
    14641 ggttgcgatt aaacttgatg aaatcaacca gacgcaggtt gtaaaagaca ttctcgacca
    14701 tgcgaaactg acagaccgac acaagcagat tttgaagcaa cagtcagtaa aagaacagga
    14761 cgtgataaca acaaaaaaat aactcaaata caaattcatt gaatatagag aggagaacat
    14821 ttttatgaat tttggacaaa acct
//
LOCUS       DAEGIQ010000064        14708 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000064 DAEGIQ010000000
VERSION     DAEGIQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.065"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(187..753)
                     /locus_tag="KMT45_001797"
     CDS             complement(187..753)
                     /locus_tag="KMT45_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBE7005259.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(753..1709)
                     /locus_tag="KMT45_001798"
     CDS             complement(753..1709)
                     /locus_tag="KMT45_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBE7005260.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1709..2365)
                     /locus_tag="KMT45_001799"
     CDS             complement(1709..2365)
                     /locus_tag="KMT45_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBE7005261.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2735..4753
                     /locus_tag="KMT45_001800"
     CDS             2735..4753
                     /locus_tag="KMT45_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBE7005262.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4823..5029
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5180..7594
                     /locus_tag="KMT45_001801"
     CDS             5180..7594
                     /locus_tag="KMT45_001801"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBE7005263.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7942..9495
                     /locus_tag="KMT45_001802"
     CDS             7942..9495
                     /locus_tag="KMT45_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBE7005264.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9876..11660)
                     /locus_tag="KMT45_001803"
     CDS             complement(9876..11660)
                     /locus_tag="KMT45_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005265.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11675..13396)
                     /locus_tag="KMT45_001804"
     CDS             complement(11675..13396)
                     /locus_tag="KMT45_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005266.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13496..14257
                     /locus_tag="KMT45_001805"
     CDS             13496..14257
                     /locus_tag="KMT45_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBE7005267.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4780 a         2716 c         2924 g         4288 t
ORIGIN      
        1 gtcctgcatc aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgatttgg
       61 ttggacttat taccgcagat ataagctttt gagcaccgtg aataaagagg ttatgaaatc
      121 aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca tatcatttat gttttgcgtt
      181 ttttatttat ctttttttct caatacaata taaaataggc ggctggttct tttggttgat
      241 aaaccgataa ttcagcacat tatatgtttc ctgtggcagt ttggaacaaa aagaatcaac
      301 catatccagt tctttttctc caccttcatg accatagtag acgacaagga tcaatcgtcc
      361 gcgaggcaca agtcgtttca atatttcttc catagctgtg cgtgtagtct ctggcatagt
      421 gatgattgct ttatcacttt ttgggagata ccctagattg aaaatccctg ctttgagatt
      481 ttgttgttca gctatcactt ctcctaacgt ttcgtggcct tgtagaaaca atgagacacg
      541 ttcttctaac tgacgctgtt ctagcctttc acgagtagca tcgattgctt gttgctggat
      601 atcaaaacta tagacatgac cagtttttcc gatgtgttct gctagaaaaa ccgtatcgtg
      661 gccattgccc atcgttgcat ctacgacgtg atcccctggt tgcaagattt cttgcagtaa
      721 tgtatggctg aaatgcaaag cagtttgcaa catcagacgc tcaccttttt ccgtacatca
      781 tattttcctt ggaagctatc tctgcgaaga agttcttcat caattgcatt caagacttcc
      841 cattttttca agctccacat tggtccaatc aaggaatccc acggcgcatc ccctgtcaaa
      901 cgatggatga tgatctcttt tggtatcatt tccagctggt cgcaaatgac attgacatag
      961 tcaggacggc tcattaattg aaggcgacct tcatgatagt cccgcaacat tctcgttttg
     1021 cgcatcagat gcatcaaatg gagcttgatt ccttggatat ctgaatctaa aatggtgcgt
     1081 ctgacatttt ctttcatcat ttctaaactt tcgcctggta atccgttgat caaatgcgta
     1141 caaacacgga tattatgttt gcgtaattta gccacgccat ctaaataagt ttgataatca
     1201 tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg tttgcaatcc aagttctacc
     1261 cataaataca tccgctcgtt cagttcagca aggtaatcaa ctacttcatc tggcagacaa
     1321 tccggtcgtg taccgactga taatcctacg acacctggta aattgactac ttgctcaaat
     1381 cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa aattttggaa ataaacgatg
     1441 tattgttgga catgaggcca tttttgatgc atcatgtcga tttctttctg gaattggatt
     1501 ggaagagggt cttcaggtgc aacgatcatg tctcccgaac cagatacact gcaaaaagta
     1561 caaccgccat gggcaacagt cccatctcga ttcggacagt caaatcctcc atcgatcggc
     1621 accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt aattccaaga atgatatctt
     1681 ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt ctttctgtaa gagttatcta
     1741 tttgttttct tttttctcta tacactggat aagtcaggca cagccaagaa agactgagta
     1801 agtagccgcc gatgatatca gtaggaaaat gaacgcccag ataaatgcgg cttatcccga
     1861 cacttactat aataaacatc atcagcaatt gaagcaatgt gcgtaaggtt cttttttgaa
     1921 caaaagctgg aagcagaaat agtaaagaac cgtaaagaat catactgcct gctgcatgtc
     1981 cactagggaa actgtaactt gtttcaacta ctaagtgcgc caatgaagga cgctctcgca
     2041 tgaaaaatag tttgatcacc gggttgatga ttccagcaat tacaataaat ccagaaacga
     2101 accaaaatgt ttctgccctt tttttcttat accacatcca taaagccgca caagcaaata
     2161 aaatgatgac ggtaagagga ttaccgaatt tagtcataaa gagttggtag ctgttcagat
     2221 caggataaga agctctgaca atgcctgtaa accactgatc gaaaccagaa agccacgaca
     2281 gatggaactt tacgatccag cccaaaatga aaaacaggac aagaaagaag ctaccagcca
     2341 cctgaaatga tgttttatat ttcatacaat tcctcctttg aagcaatgac ttattatagc
     2401 atttttggtc cagcgaaaaa aacggtaaag agataaaacc aatgtatgat tcctctttga
     2461 ataaccaata ttcttataaa aaaaataact gtaatcggaa agacattttt tggtaatgca
     2521 aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat ccttttaaac aagaacgtag
     2581 acgtaggaag aacttgaaaa tgaacagaag ttgttcatta cggtaaatta caatatgatt
     2641 acaaaaaaca acaaagctct taaataactt ttaataaata ttaagagaag ttataattat
     2701 taataataga taaattgaag ttggaggata gaagatggaa aacattgcaa gaaaagaacg
     2761 gagacgttta gaacagacca aacgttttcg aaaagcaaaa cgaggggcag caatcgtggg
     2821 aactgcgatg gtaggctgtt ctgtcgctgc cccattagta cagccggttc ctgttcaagc
     2881 ggatgaaaca cctgttcagt tcggcgcaag aatcaataca gcggcattta tcacagaaat
     2941 tgctacttat gctcaaccaa tcgctcaagc caacgatcta tatgcatcag tgatgatcgc
     3001 acaagcagtt gtagaaagcg gttggggcag tagtgcgtta tctcaagcac cttattataa
     3061 tctttttggg ataaaaggca gctatcaagg acaaaccgtc tacatggata cgcttgaatt
     3121 tttaaataat caatgggtaa ataaaaagga gcctttccga caatatcctt cttttgctga
     3181 atctttcaat gacaatgcct atgtacttag aaatacttca tttggcaacg gttattacta
     3241 tgccggtact tggaagagca acacgaaatc ctatacagat gcgacagctt gtttgacagg
     3301 gcgttatgcg actgatcctg gttacgcagg caaattaaat aatatcatcg caacttacgg
     3361 attgacccaa tatgatacac cagctagcgg aaacgcaggc ggtggagcga ctacaggtgg
     3421 caataatact ggaagtgcag gtagtactgg cggttcaagc aattctggta atacaaactc
     3481 aggttcagca acttcgacca cttatactgt ccaatctggt gattctgttt ggggaatctc
     3541 aaataaattc ggtatcacaa tggatcaatt gatccaatgg aacaacatcc aaaacaattt
     3601 catttatcca ggacaaaaac tgacaatcaa aggtagccag gaaaatggct cttcgacgaa
     3661 taattccggt aacaatacca actcttcagg aaatgctggt acatcaaacg gaggacaaac
     3721 aactggtgcg aaatacactg ttcaatctgg tgattctgtt tggaagatct caaatgatca
     3781 cggaattacg atggctcaat tgattgaatg gaacaatatc aagaacaatt tcgtttatcc
     3841 aggacaacag ctaatcgtat caaatggcgg ttcttctaat gctggagcaa atactggctc
     3901 aaatactggg aatacaagtg gctctagttc gtccagctca agttcttcta atacagcttc
     3961 aggaacgaaa tatacagtta aagccggaga ctctgtttgg agcgtctcaa ataaatacgg
     4021 aattacgatg gctcagctaa tccaatggaa caatatccaa aataacttta tctacccagg
     4081 acaacaactg actgtttcaa acagtggatc aacaaacagc acgtcaggta atacaggttc
     4141 gtcaaccaat acaaataaca gcagttcagt taatactggc agcagttcgt ctcaaggcac
     4201 ttataccgtg aaagcaggtg aatctgtttg gagcgtggca aataaaaacg gcatcacgat
     4261 ggatcagtta atcgaatgga ataatatcaa aaacaacttc atttatccag gacagcaatt
     4321 gatcgttaaa ggtggaactt cggcaaatac aaatacaggt tcaactactt ctgcagcgaa
     4381 accaaataca ccgaatacgt ctgctacgac aagtacatca tccactggta atacgatgta
     4441 taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac aaacatcata tcacgatgga
     4501 tcagttaatc gaatggaata atatcaaaaa taatttcatc tatccaggac agcaagtgat
     4561 tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca gttactggtt cgaaaaccta
     4621 tactgtgaaa tctggagaat ctgtttggag tgttgctgac agtcatggaa ttacaatggc
     4681 tcaactaatc gaatggaaca atattaagaa caactttatt tatccaggtc aaacgttgat
     4741 tgtagctaaa taaaatagct gttcgaagaa ataacgattg aattccacaa aaatatttgt
     4801 tactataagg ctacttaaat atgacaacac ttgaaaagga agtagtaaaa agactgaagt
     4861 ttcagagagc gcatggctgg tgagaatgtg cacggatgct tttgaactcg cctttgagtt
     4921 gcccttgctg aatacccgag taagcaagcg gcggtgacgg ccgttacaac gttcgaggcc
     4981 ttttcactaa ggtaaaatta ggtggtaccg cgtagattac gtcctataaa aagagcaatc
     5041 tttttatagg atttttttat ttcttttgtg aagtttgaga aaacaaagca aaaggaaata
     5101 aaaataaaca gattctgccg gttttttcgt tatatttata atagaaacaa gtgttttcaa
     5161 tagaacagga ggacagcaaa tgagttacaa tcataaagaa attgagaaaa aatggcaaaa
     5221 atactgggca aaaacgaata ctttcaacac ccatgatgat cctgaaaagc caaaatttta
     5281 cgcactggat atgtttccct atccatccgg tcaagggcta catgtagggc atcctgaagg
     5341 ttatacggca acggatatcc tttcacgtgt gaaaagaagt caaggataca acgtgctgca
     5401 tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa tatgcgctag atacaggaaa
     5461 cgatccagct gagtttaccc aaaaaaatat cgaaacattc cgacgccaaa tcaattcttt
     5521 aggatttagt tatgactgga atcgcgaagt gaatacaact gatccggagt attataaatg
     5581 gacacaatgg atctttacga agctttatga aaaaggtcta gcttatgaag cagaagtagc
     5641 ggtaaactgg gtacctgaat taggaacagt tatttcaaat gaagaagtga tcgatgggaa
     5701 aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg cgccaatgga tgctgaaaat
     5761 cactgcttat gctgatcgct tattggatga tctagaatta gttgattggc cagaaagcat
     5821 taaagaaatg caaagaaatt ggatcggtcg ttcagtagga gcaaatattg aatttaaagt
     5881 ggctggtacg gataaatcct ataccgtatt tacgacacga ccagatactc tattcggtgc
     5941 aacttactcg gttcttgcac ctgaactaga tcttgttaga gaaattacga cccctgaaca
     6001 aaaagctgct gtagaagctt atatcgaaga gacagctaaa aaatcagatc taaagagaac
     6061 ggatcttgca aaagaaaaaa caggagtatt tactggtgct tatgcaatca atccagttaa
     6121 tggcaaggaa atcccaatct ggatcgccga ttatgtatta tcttcatacg gcacaggagc
     6181 catcatggct gttccagcac atgatgaacg tgactttgaa ttcgctcaaa ctttcggact
     6241 agaaattctt ccagttatcg aaggtggaga tgttgaaaaa gctgcttaca cagaagatgg
     6301 tacgcacatc aactctgaat ttctagacgg catgaacaaa caagaagcaa tcgataaaat
     6361 gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa gtcagctatc gcttacgtga
     6421 ctggctattc tcccgtcaac gttactgggg tgaaccaatc ccaatcatcc attgggagga
     6481 cggaacagtc acagctgtcc cagaagaaga gctaccatta cggttgccta agacaaaaaa
     6541 catcaagcca agcggtactg gagaatctcc attagcaaat atcgaggagt gggtcaatgt
     6601 cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg aatacaatgc cacaatgggc
     6661 aggaagctca tggtattacc tgcgttatat cgaccctcat aacaaaaaag aactagcaga
     6721 ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac attggtggag cagaacatgc
     6781 ggtacttcat ttactatatg cacgattctg gcataaattc ttatacgata tcggtgttgt
     6841 tcctacaaaa gaaccattcc aaaaactata caatcaagga atgatcttag gcgaaaacaa
     6901 cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct gatgatgtgg tcgaaactta
     6961 cggtgcagat acactgcgca tgtacgaaat gttcatgggg ccattagatg cttcgattgc
     7021 ttggagcgaa aatggcttag aaggaagccg taaattcttg gatcgtgtat ggcgtttgat
     7081 cgttgatgag aacaataaaa tgcgtgaccg tatcactaca ttgaatgatg gcaagcttga
     7141 taaagtgtat catcaaacag tgaaaaaagt gacagaagac tatgaaaatc ttcatttcaa
     7201 tacagcaatc tcccaattga tggtctttat caatgaagcc tataaagtgg acgctctgcc
     7261 ttatgaatat atcgaaggat ttgttcaact actcgcaccg attgctcctc acatgggtga
     7321 agaactatgg gcaatccttg gaaatgatgg tggtatttct tacgcaccat ggccaacata
     7381 cgatgaagca gcacttgttg aagacgaagt agaagttgtt ttccaagtaa acggtaaagt
     7441 tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat gagctagaaa aagcagcatt
     7501 agccaatgaa atagtgcaag aatatattga aggcaaaaca gtcagaaaag ttattgcggt
     7561 accaaacaag ttagtcaata tcgttgctaa ttaattggct ctttgtcaat aaggactgat
     7621 gagttgtgca aaatcaaatc tgggccagaa tgaacttcat tctgtctcag attttttgtt
     7681 atttcaaatt gattaattga ttgatcaaaa aggttttgat tttcatgttc tcaaacacgt
     7741 tcaatgtctt tttcttctga atggtcttgt aagagatctc tgtaatcatc aaatttttgt
     7801 ctattaatct tagtattttt tgatgtataa actacctgaa attgagagta cataccaaat
     7861 tcataaattt tttattagtg ggactagtga ttgtatggat tttgacgcat aatgaatgaa
     7921 gtatgagaaa aggagagaca aatggatcaa gaactattta ttatcgaagt attatcagct
     7981 aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa gaataatgtt caagctttta
     8041 agcttagtat ttttctctgg tttgcttttt ttcggagctg tatcgttcct atatcgccat
     8101 atgagtgcag tagtccgttc agctgaaggg atgcaaaaat taaataattt gtatcacttt
     8161 tcgtggggag ttggcggaat aacaattgtt gtgcttattg tttttcttct acttatttgg
     8221 ctccgttacc atcaattgtc gcgtcatttc gatcaatggc ttagtcaaag agcacaacag
     8281 tttttacata ataaaccaac ttctcaagat gatgactatc tttattttga gaagacttcg
     8341 acgagtcaag cttttcggat acaaaaaagt gattgccagt tattgctgag atcacttgat
     8401 gatttaccta tctacttagg cgaaagcaaa tcaaaattcg gaatgaacac attccatatt
     8461 tttttactag aaaacgaacc cagatgggaa aaaagccagt cttcgcctgg tggatacctg
     8521 acaactaaga tgagtacatt agtcactggt tttttcctca ttcttttagc tggaacaatc
     8581 tattatggga gatacactca gccaaaaatg tcttctcata taaatgctgc ttctcacatg
     8641 acaatgaact caactaacga tacgcatata caaaagacaa atgaagggtt gttagctgat
     8701 cctaagcaaa aggtaaattt gacaacacaa aagataaatg atctcaaact tgatacagag
     8761 acgcactctc tctatatgac aacagatagt ggtgctaatt gggtatttgt gccgattgat
     8821 tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg ggctgattcc tgaaggttat
     8881 tggatggaca acacatatga tatttccgcc gatttctcat ggtttatcta ttcttctggg
     8941 gatgatgtac gtctgctgac ttcaagagat aatggtaaaa catggcaaac gtctattatc
     9001 acccaatcag ttcagccgat acgttatcga aaagcggtat tttatggcaa cgatggtcta
     9061 tttatctgta catcagccag tgggatgtct gcagaaactt tgtacattta cactactatg
     9121 gatggcggaa tcacttggat aaaaagtgga agtacaatga tcaatcagcc agtacaaaat
     9181 gtcagcttcg ttacaacttc tttaggtttt gtttcgacac gtgaaagttt gttttataca
     9241 aacaacggag gacgctcatt caaagaatca gttctgtcga ttccggaaaa ctatcagaca
     9301 ggggggatag atctttttca atcaccaaat gaagttactc aaataagcgc gaatacgtta
     9361 gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg cagggaaaat gtttgcctgc
     9421 ctcttccgtt caactgataa cggggaaaca tggcaattcg ttcagcaact atcgcaagta
     9481 gaacaaggag attaaagaag agagattgtg agcctgacgt tcagcaccga gtcacaagaa
     9541 acaaattcaa aaaacagctt ctcatatttg ttgaatagtg taactcgtga ttcggagttg
     9601 acaagagcaa caagaagcga ttttgaagta tagcttctcg tattttacaa gatcgtgtct
     9661 tattgccgat aggttgtctc ttgaacaccg tttattcgta aaaagggatt gtgacaagtc
     9721 ttttgtcact acttaccgaa cgaaactgaa ggagctatcc aaaaagtatc attagtatta
     9781 ttctttgtgt ttctagtggc accactagta catttaaaaa agctatcgat catggactaa
     9841 tcatacatga tcgatagctt tttagataat gtttgttacg ctatctgaaa ttgactatta
     9901 taaagactgg cataaagcgt tggtgattgg agtagttcag aatgtgttcc tttttcgacg
     9961 atatcgccat tcttcattac aaggatcatg tctgcatttt caatcgttga caaacggtgt
    10021 gcaatcacaa aacttgtcct atcttttgtc acagcttcca ttgcttgctg aatcaatgct
    10081 tctgttcgtg tatctacgct agaagttgct tcatctaaaa taacgacagg aggattcgct
    10141 agaatgactc gagcaattgt caataattgt tgctgtcctt gagataacgt gccgttttcg
    10201 ctagaaatga tcgtatcata tccgtctggc agtgttcgaa taaagtgatc acattgtgcg
    10261 atttcagccg cttcgataat ttgttctctt gtggcatcca ttttcccgta ggcgatatta
    10321 tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa ccatcccaaa agaacgacgt
    10381 agttctccac gtgaaagctt ggtaatatca agaccgtcga attcgatctt tcctttattc
    10441 agttcataaa aacgcatcag cagattgact aaagtcgttt ttcctgcacc agttggacca
    10501 acgatcgcta ccgtttgttt tggctttact gaaaaatcga cttgattcat caatatttta
    10561 tctgatgtgt aaccaaactg tacatttttg aaagctattt ttcctttcgg ttgttctaaa
    10621 agtcttgcat ctggaaggtc tggaatttca tctgtttcat ctaatatctc aaatatccta
    10681 tcgattgctg ccattgcgga ttggatcgag ttgatcacat aagaagcggt agaaattggt
    10741 tcagaaatct gattgatgta ctgaagataa gcttgtaaga agccgatcgt cacaccacct
    10801 gatagaacaa gcatagcacc gaataccgca cttatgatga aggcgagttg attaatgaaa
    10861 cgaatggccg gatagatagc acagttcata aattgagcac gtttaaaagc gttgtattgc
    10921 tttttattta cttcgtgtat agcagcttct gcatgagctt gctgattgaa tgttttgacc
    10981 actagattac cagctaagat ttcttccatt cgattattta aaacacccag ttcatactga
    11041 ttctgttcag aaatctgttt gtttttgttc gcaatcttag ttgtaacaat tgaactgcca
    11101 acgatcagca ataagacgag caaagtaagc ttgctttcga tataaaacag catcagacca
    11161 gcagcagcta ttgttacgac agaagtaaaa aattgattga tacctgtcaa taacacttga
    11221 gaaagttgat tgagaccggt agttgtccga ctaatgatgt cacctacttg atgattgtca
    11281 aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt cttttcgcac cctcaatgtg
    11341 atcttttcgc ttaacgctgc cattgtccgt tcctgaataa atgaagtgat gctgcttaag
    11401 actgaacagc cgatcagcaa taagacaggg aacaacaatg cttgtttgat atcactgaaa
    11461 gaaagctgtc caatacctgt ttttcttatt tctgataaga gatcatcgat cccgattccc
    11521 atgatcaagg gcatggctac gactaatagg ttcccgaata aactgcatag aaacaatccg
    11581 taaaaatatt tatgttcagg tttgatcatt ttccaaaaac gtatcaaagc acgcgtgttc
    11641 aacgtttttt tcttttccat cgtatccctt ctttctatat tccttgtgat tttgcaaact
    11701 cctgataagt ttggcaatta gacagtagtt cttcatgagt accttgtcca acgatttttc
    11761 cttcatctaa aacaatgatg ttatctgctt gttggattgt actgaggcgt tgagcaacaa
    11821 tcacgagagt tttctccgac atgtgtttat gaagggctgc tcttaatgct gcatctgttt
    11881 tatagtctaa agcggagaaa ctatcatcga atacatagat ctctgctggt ttgatcaacg
    11941 ctcgagcaat acatatccgc tgtttctggc cccctgaata attagaaccg ccttgagcaa
    12001 caaagctgtc caatccgtca ggaagggtgt ccactagttc ttctaattga gcgatcttga
    12061 tcgctttaga caactcttct gttgaggcat attcatttcc catctgcaag ttgcttaaaa
    12121 ttgtcccgct gaataagaaa gccttctgag ggacataact gatgtgggag cggatctgtt
    12181 cttgagtcat ttgagtaaga ggaataccac caagggttac ttttcctatc gtagggtcac
    12241 taagtctcaa gagaagtttc gccaaactac tttttccaga tccggtcccg ccaacgatcg
    12301 ctgttgtttt tcctttaggt attgtgaaat caatatcttc tagaactgga tcagagattt
    12361 cagtatattt aaatgtgaca tgttcaaaag cagctgcatt tgtctgatca aaggttggtt
    12421 cagctactgt tttgatcgtc tcttcctcaa tttcgatctc tgcttgcagt atttcttcta
    12481 tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa taccatagct gcgatcatca
    12541 agtaagcaag tgtcagcata gagtattcga ctacagcagt cacgacaccg atttgtaatg
    12601 ctccttctgc tacaagattt ccgccaaacc acaaaataga agcataaacg attcccatga
    12661 gcaaaaaagc aattggagtg acccaagcaa atattcggtt cactttgatc atttgattag
    12721 catagtcagc aaagctctta ttcgttcgct tttcttcata tgattgatta tcaaaggcac
    12781 ggatcatatt aatcccagta aagaattctc tcaatgtgat ggtgatccga tccatttttg
    12841 gctggatact caaagataaa ggtgcccctc gtttcatcag tcgataaaca atccatacat
    12901 aaaaaacaac agagatcaat gtaacccatg ttagtaatag agaataacga aacatcatga
    12961 gaatggtgaa aacgcaaata accggtgctg gtaagatcat ttggcaaaat aaaacaatca
    13021 tttgctggac attatcgaca tcattcgtca ttcttgtcaa aagagatcca gaactaaaac
    13081 gactgatatc cttcattgac agctgttgga atttacggaa atatttctcc cgcgtttgga
    13141 atccaaacct agcggaagcc aatgctgaaa agtaactaga ggcaactgca gcgattgttc
    13201 ctagaaacgc tacaagcagc atttcaactc caattttttt tataactgag gcttcgccac
    13261 ttgctatacc tgtgtcgatc agctttgcta ctaattttgg tacacctaat gtaccggcga
    13321 tttctaaaca gatcatcaca aaggtaagga gagcgagtcc tttgtttttc tgtaagaatg
    13381 tttttaagat agacatatta ttcctccttt tttcctgata cttttcgatt ctataccttc
    13441 aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa aagaggtgag aaaaaatgaa
    13501 aaaaatgtat acgattggcc aagtatcgga actatttgat ttgccaaaat caacgatccg
    13561 ctattgggat gaacaaggat taatccgttc aagtagacaa gaagagaatg attatcgatt
    13621 gtttgatatc gatgatattt ttatgcttta tgatatcgtt ttttatagaa aattagatat
    13681 accgatcaaa cagatgaaga atttatatgg aaaaacattg acggagttat atgagacact
    13741 tgatgaaaca gaaagaagaa tccataaaga actggtggtg atgaaacaaa aacaaaaaga
    13801 gataagggag caaaaaaaac agttgaaatt gatgatcgat acaaacgagg aagaatttcc
    13861 tgtagaagag atcccttttg actgtatgat ctcaacggag tttgaagata ttgtggaaat
    13921 caaaaaattt ctacctaact attcaagctt tgggatgatg agtatgccag cttccagttc
    13981 acagacgatg tatggatttt ttatcgaccc tagtgaagta catttattta ctcaagacgt
    14041 gatttgggag aaaaaagata cggctgttta tcgtcgattc ttattgaaat ccgagatgaa
    14101 ccatgcggaa cgtaataata ttttagaaat cagagaaaga atgtatgaaa aaggatggaa
    14161 aacaggagaa gtgatcggcc agtatctatt gacgaatacg gatgaaaaca atatcagaac
    14221 tgaatattat catgcatgga tagaaatgaa aaaataggaa atatgtgttt aagataactc
    14281 gaaaatgatg gttgttttag ttataaagaa aaattttctg gttcgattac gggtttaatg
    14341 tgggggatta ttagtcgtgg atagatagaa ttttagtttt ttagaggaaa tatgtcttta
    14401 aaaatggaat ggttgcaatt cttagcgata tatacgatgt aattgtcctc ttaaaaaagt
    14461 atggaaaaat aattgtattg ggtgttatgt ataattcatg gtgaactgag actaatgaga
    14521 agaaaaattc atattgttat gagtgagatt gtatcagatg agaatgataa attaaatagg
    14581 tgcataaaag cattacactt aaaatacatt gttgcttaca ttattacaat atgttaataa
    14641 ggcaaaaata aatagatgga ttgaataaac atatttttta atcaaatagt ccctactgtg
    14701 actttcct
//
LOCUS       DAEGIQ010000065        14675 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000065 DAEGIQ010000000
VERSION     DAEGIQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.066"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            941..2101
                     /locus_tag="KMT45_001806"
     CDS             941..2101
                     /locus_tag="KMT45_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005268.1"
                     /translation="MKNYNRISANNQLPMIESLRVPTNADKKIYGHVVVPEGVEAKPR
                     DAWTGEVEVTLRITYADTTKSSVEITALTQGQTDENEGAGYNPWGEGEQGGLFEVQVP
                     NDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVDVIPPEPANLSTTLINAASRTIT
                     GTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPENQLFAGDVLEIVLNDQAGEATAK
                     GVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKIVVEGGLSFLAPSKLDFGQIKAT
                     GLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQPFTNDAGFSMQDVLYFKKGEEY
                     QLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLVLTKENQIAGNYSAEIT"
     gene            2148..2693
                     /locus_tag="KMT45_001807"
     CDS             2148..2693
                     /locus_tag="KMT45_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBE7005269.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            2721..3176
                     /locus_tag="KMT45_001808"
     CDS             2721..3176
                     /locus_tag="KMT45_001808"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBE7005270.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            3173..3931
                     /locus_tag="KMT45_001809"
     CDS             3173..3931
                     /locus_tag="KMT45_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBE7005271.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(4017..4757)
                     /locus_tag="KMT45_001810"
     CDS             complement(4017..4757)
                     /locus_tag="KMT45_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7005272.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            4818..6071
                     /locus_tag="KMT45_001811"
     CDS             4818..6071
                     /locus_tag="KMT45_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBE7005273.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            6356..6619
                     /locus_tag="KMT45_001812"
     CDS             6356..6619
                     /locus_tag="KMT45_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBE7005274.1"
                     /translation="MEKQANPYDHKKMLTQQLRELEQDESIHRKAYNQVPPKVEYSLT
                     EEGKSLREILVALSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            6742..7068
                     /locus_tag="KMT45_001813"
     CDS             6742..7068
                     /locus_tag="KMT45_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBE7005275.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            7136..7849
                     /locus_tag="KMT45_001814"
     CDS             7136..7849
                     /locus_tag="KMT45_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBE7005276.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            8159..9010
                     /locus_tag="KMT45_001815"
     CDS             8159..9010
                     /locus_tag="KMT45_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE7005277.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            9131..10996
                     /locus_tag="KMT45_001816"
     CDS             9131..10996
                     /locus_tag="KMT45_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE7005278.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            11010..12452
                     /locus_tag="KMT45_001817"
     CDS             11010..12452
                     /locus_tag="KMT45_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBE7005279.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            12560..13261
                     /locus_tag="KMT45_001818"
     CDS             12560..13261
                     /locus_tag="KMT45_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7005280.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            13433..13912
                     /locus_tag="KMT45_001819"
                     /pseudo
     CDS             13433..13912
                     /locus_tag="KMT45_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13950..14429
                     /locus_tag="KMT45_001820"
     CDS             13950..14429
                     /locus_tag="KMT45_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HBE7005281.1"
                     /translation="MFLDSGVVLKETELERIEYTDFGLGAIETEGLNLFVYENNERYC
                     GKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKLSCIIPKGQE
                     RYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDSFTNPAIYR"
BASE COUNT         4918 a         2481 c         3153 g         4123 t
ORIGIN      
        1 aaagatcaat attaccaatg acaacaatcg cggaatcatc caaatgagag gttcaggatc
       61 gaaagtcact gtatcgaatg gtgggaaaat caccatcaat acagcaacta cgggcggatt
      121 tcgcttgcaa ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa
      181 aatggctgga gatgacgatc aacccgggaa caatgggatg cgttttgtag gaaaaggcct
      241 ttctttgatc gtagaaggag ccggatcagt tatcgacatc gataagcaga gtgggcgttc
      301 cagcgcagtt cgttttgaga atggtacgca aaagctgaca gtaaccaatg gaggctcatt
      361 gagggtaaga aatacaggtg atggcaaatc ttacgtgaac agactcaatc aaggaaatca
      421 agcgatccaa ttttatgacg cttctggttt tctagaaagt ccaggttcag ctgattttac
      481 agtcactgga gaaaaatcaa atatcgatat acgcgcagat tccggagcaa ctgtagatac
      541 aactggagat attgatttaa attttacggc aggtgaaaaa acttatttag tcatggtcgg
      601 gaaaacaggg gaagataaga ccggcatcat cagtggggat gtgttgaatg tggacttaga
      661 atctcctacc tattttgatt tccaaaacaa acggattgga agcgagacca atggcatagg
      721 cggctgggta ttccaaggaa atggagattc ttctttaaag atcgcccatt cagaaatcgc
      781 tttatggcga aaaacaggcg caacattcga taactttgaa aaagacccgg attatttttc
      841 tgatatgaca gacttatcga tcaatgggac cgacttaggc aatttcgtat caagccctag
      901 tgcaggttta acaaatgaat ttgctcagac aggtgccgga atgaaaaatt ataatcgaat
      961 cagtgccaat aatcagctcc cgatgattga atccttaaga gtgccaacga atgcagataa
     1021 aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg
     1081 gacaggcgaa gtagaagtga ctttacgtat tacttacgca gatacgacga aatcatctgt
     1141 agaaattact gctctcacgc aaggacaaac agatgaaaat gaaggggcag gatataaccc
     1201 atggggagaa ggcgaacaag gcggcttgtt cgaagttcaa gtacccaatg atgagagatt
     1261 aaaaacggag gatcaagtgc aagttgttgc tgctaaaaaa gtaagaggtg gccaagaagt
     1321 gacagaactg gactctgcca aaacgacagt cgatgtgatt cctcctgaac ctgctaattt
     1381 aagtaccaca ttgatcaatg cagcttctcg cacgatcaca ggtacaggtg aagcaggagc
     1441 cttggtttca ttgaaaaaaa atcaagctgt tttgtcagaa acaactattg atgatacagg
     1501 ggaatttgtt ctttctatcc cagaaaatca actttttgca ggagatgtat tagaaattgt
     1561 attaaacgac caagcaggag aagctactgc taagggtgtg cagaacaagc caagtaccaa
     1621 cgatgaaatt gggaatcaca atccaagtga tacgccagtt gtttatcatg atgccccgcc
     1681 ttttgcacct gctacgaaga tcgtagtaga aggtggtctc tcatttcttg ccccttccaa
     1741 actcgatttt ggtcagatca aagcaactgg tttgacacaa gaagcatttg gccaaatgga
     1801 tatacaggaa aaattatgga tttatgatca gcgagaaacg aaaaatccgt ggatcttaac
     1861 gataaaatta tctcagcctt tcacaaatga tgcaggattt tctatgcagg atgtcctata
     1921 tttcaagaaa ggagaggagt atcaattagt gaatgaagaa gaactggaaa ttgtcagagg
     1981 acaaaattca acgaatgtgc aagcagatta ttcagaatat ttaaataacg gtcgggcgtt
     2041 taaacttgtc ttaaccaaag aaaatcagat agctggtaat tactcagcag agataacata
     2101 gacattagca gatgcgccga cagaataaag atcaaggagg atgattgatg aatcgaatat
     2161 ggaataaaag tcatcgaaac aactgctctt tacttatgat ccttctcctt ggattcatcc
     2221 tttggcagac acctgtccat gcctccgaat tcaattttgc ggtagatttt gaattaccgg
     2281 ctaatcagat tgataagaac aaaacatata taaatgtaag gatggagcct gggaaaacac
     2341 aggaaatccc ctacacggtc tttaaccgaa cagataaatc agtaacagtt gagacattga
     2401 tccactccgc tacgacgaat tcaaacggtg tgatcgaata tggagaatca aaaagtaaat
     2461 ttgatgaaac attaagttat gatatgaaaa acttagcaaa aacagtggga gaaataactg
     2521 ttcctcctca aagtgcggtc actgagacac tttcactgaa agcgccagaa aaagagctgg
     2581 ctggtgtggt cgcaggaggg atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa
     2641 gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc aatcttgatt tgaaatagcg
     2701 aaaaagagtc ttccccagat ttgaaattaa aaaaagctta tgcggatcaa agcgatggaa
     2761 gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc ttatatgttc ggcttgacta
     2821 catcggttga aatctacaaa aaagaaacaa aagagcctta cctttcaata aaaaaagaaa
     2881 aaatagatgt agcacccaat acaaattttg atttacttgt cccattagaa ggaaaaaaat
     2941 tagctccggg acattatcaa gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc
     3001 tccataccga ttttgaaata actgatgatg cagcggacca attgaatgaa aaggatattg
     3061 atttaaaaaa tcagccagaa acatttgact ggcgatggct gatacttgtt ggagtcattc
     3121 ttattggctt atttgctgga ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa
     3181 aacaattgca ttaagtaccg ttctagctgg aagtctatta ttatgtacac aaagtgtttt
     3241 tgctgagaat aagagctcag ccactacaga aggaacgatt tctgtgacca aaccagatgc
     3301 tgcaaatcct gaagacgttg ttccagttcc tcctgttgat ccaaatgatc ctgatactcc
     3361 ttttacacct gacgaaccaa atcagggaac atccggatta ctgacgattg accaagctcc
     3421 agatcttgat tttggaacaa tcaagttagg tacagcaaaa acagtatatg ctgcattaca
     3481 aagcggaacg gacagtactg gagcacaaac acaagtacag aatttcgtac aagtaacgga
     3541 taaaagcgga aattatgccg gttggaaatt atcagtcaaa cgaacagaat ttattggtac
     3601 aacatctgat acgaataaac taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg
     3661 aaatagcgca actaatagca gtggaatgcc atctaccgtt tctggaactg gcgctgaagg
     3721 aatcgaaatc ccagcagatc aggcagtaga tctagtaact gcaggagcaa atgaagggat
     3781 tggcacatgg atttattcat taggagagaa tgtggaacaa gggagacaat cagtggaatt
     3841 atttgttcct gtatccaatt atgctgtaga caactacgtt tcaacattca catggtcgtt
     3901 aacagatgct cctacagaag aggaagcgta aaatcatatc aacgacttca gggagtggag
     3961 ccgattcaag gcccactccc tgaaatctgc tatgcttacg tctcagctct tattcatcaa
     4021 ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg tcttttcaaa gatctttcga
     4081 ccaacgagta aggagtcacc gttgaaaaat tctaacacat aatgagcaga atcaattttc
     4141 tgcaattgcg ataagttgat acagtaagat tttaaaagta aaaagtaagc atacttttct
     4201 aactctgtct tcaattgttt taaagaagta ttcactaatt tatcttctgt agttgttttg
     4261 accataactt ggtttttcaa acctttgata ctttcaatat ataaaatttc ccgaggatac
     4321 aggacgagac ttccaccttt ttgatcaata aagattttat cgacatttac gatctgctgt
     4381 tctttccaat tgatctgttc tagcacttga cgaatttgtt cagataaatt cctgtctttt
     4441 ctgatatatc caatcggaag gattttccga ttgatgatct ctactgcaaa attcgtataa
     4501 gcggtgaaaa aaacaatatt actatgtgca ttttttttac gtatttgttc ggcgacatca
     4561 atacctgtga gtgattgctt caagtcgata tcgataaaga aaacatcgtg attatcaata
     4621 gtgcccaact ggatcaactg attgatttga gagatagaag tgagctgcac gatattctcg
     4681 atatgatgat tgaccaattc tctgatgatc cattcgctgt gaaactggtt atcttctatg
     4741 ataaaatact ttttcatatg caggctccta ttaaaaaaat atgtgtacat tttgaaacaa
     4801 aacaatggag gaataagatg gtcgcaacag tgacacttta tttgaccttt actttgattt
     4861 tagatttagg tatctattta ttcttttcga aacaactgct agtaaaaaac agctttggtt
     4921 gtatagcttt gatggtggtg acacttttac ttcctatccc taaagtgagc tttccttacc
     4981 taattggctt attattgatg tttctttttt attttactac gaaaaacctg tttatcagct
     5041 gtttgatcag taatcttttt tttgctatca ctggactcag cttttttctg gtttttgatc
     5101 tgatggatta tttaggaaag agacatcttg gaacagcaag tttttttgca ctacttatcg
     5161 catttatttt ctatctatta gtaaaaagta tagacaaaca gacgagattt ctcaacttgc
     5221 tttttactta tccaactgaa cactattttg gaggtatttg tattttatta agctggggcg
     5281 tgatttactt ggtattggga agtgttcact tccgctcatc taattatctg cttctatctc
     5341 ttttgtcgat agtcatcagt tttttttctt tgtttttttc cattatgttg atcagtttcc
     5401 aagtaaaaga aagacaacat caggaatcgc ttcaactgta tcaaataaat gaagaatatt
     5461 accatcatct agaggaattc aaacatgatt ataaaagtct actgttttca ttaaaaatta
     5521 ctcttcagga aaataaaaaa gaaacgttgg aatttttaga aatattagaa gatcattctg
     5581 cagctatttt tgaaaaaccg caaatacaac agctaacaaa tattgtatcc ccagctgtca
     5641 ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga gaaaatcccg atccaagtag
     5701 ttattcctgt cgaagtgaaa acaatcccga ttgatctggt tgtttttatt cgctgtttat
     5761 ctatttttat ttctaacgca ttcgatcatc gtgtagccga tcagagtccg attacgatca
     5821 atctgctgga agaaaaagag ggatcacgtt ttagtatagc gaacaaggca aatccaacga
     5881 atttgacgct atcggagatg gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag
     5941 gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa tgcagaacta aaaatcgaat
     6001 tttcaaaaca gaatcactat ttttttgtag aaatgtatct ggcatctaga aagagcaaat
     6061 cagcctactg acttactgtc aaaggataag tttgaggaaa gtctacacgg agatagagac
     6121 aacaacacag catattcaaa agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc
     6181 ttttgctatg tagttaaaca aagtagttga cacttccgct acttagtatt agtacgaaag
     6241 tatacttcat gatactaagt agataagaag tgaaaagcgg aacccacgat cgtcaaaatg
     6301 ctaactgctt tgcttacacc agataaaggg aagagcaata tcgcttattt taggcgtgga
     6361 gaagcaagca aatccctacg atcacaaaaa aatgctgacc cagcagttaa gagagttaga
     6421 acaagatgaa agtatccatc gaaaagccta taaccaagtc ccgcctaaag tagagtactc
     6481 gctcaccgaa gaaggaaaat cattaagaga gattctagta gcgttgtctg tttgggaaga
     6541 agaacgtatc gaaaaacaaa aagcctcaag ggaagaagac acgctactgc atacacatgg
     6601 tggctaccta aattattgat tttctatgac atttatttta aataaaagaa aacaggagtt
     6661 tttttaaaag catcatctat tttttatgta aaaaaatggt atgattaatc aagtaagaag
     6721 tgattggaag aaggcaagca aatgaatagt atggaaaaag tcaaccagtt tagagatgaa
     6781 cgtaattggc gacaatttca taacgaaaaa gatttggcta tctctatttc tttggaagca
     6841 tctgaattgt tagaactttt tcaatggaaa acgccggaag aagtaaaaga aacacaatta
     6901 gaacgaatca aagaagaact ggcagatgta ttgatctatt catacatgat ggcagacaac
     6961 cttcacctgg atctagatga gatcattgaa gaaaaattga tcaaaaataa tttgaaatat
     7021 cctgtagaaa aaagcgcagg tgtacgaaaa aaatacacag agttgtagaa atgtgtcatt
     7081 cggctgttgg aaagagcaaa agaaaaacag taaaatgagt ctggagggag agaaaatgaa
     7141 aaaaaaagga aaagcaaaaa gatggctagt caatatcctc ttgttcctct tgttattagt
     7201 aggactagct ttgattttca atgaacagat caaggattat tttgtcagag agacaggaga
     7261 taaatatgct atagcgaatg taacaaaaga agatctgaaa aagaataacg ataaagatgt
     7321 cagttttgac tttgatgcgg tagaaccaat gacgacagaa ggggtcatga ggtcgcaaat
     7381 gagaggaaca gatctaccag tgattgctag tatcgcagtc ccttcagttt caatcaattt
     7441 accggttttc aaaggcttgg ataatacgtc actactttac ggagcaggaa cattatctcc
     7501 agatcaagaa atgggaaaag gaaattatgc cttagcaagc catcgtgcaa cgaaccctga
     7561 attgctgttt acaccgctag aaaatttaga gatgggtgcc aagatctatt tgacagattt
     7621 agaaaatgtg tatacataca aaacattttt taaagaaaaa gttgctccta cagataccca
     7681 attactgaat gaagtcgagg gaaaagaaat tgtcacacta attacttgtg gcgacatgga
     7741 cgccgtcacg cgactagttg tacaaggaga actggaaagt gttacatcga taaaagatgc
     7801 gacagatgat atgagatctg ctttcaatct agagacaaaa acattttagg atactgggac
     7861 ataagtcgtt acagattaaa aacagccaaa ggatatgaat cgataaggaa aaaccaattt
     7921 gctgaaaaaa agagaaacgc aaagaggtgc atctctgatt tggttgtttt ctagatagac
     7981 ttgtgttcca gtttttttat tttttatatg aaaaggcttg cataaataaa aattcgatgg
     8041 tatgatgatt ttgaataaga aatggattgt tactgtacgg caggcaaaac cagaattcag
     8101 ctaagaaaca gtaggcgaat tctaggtttt tttatttttc ttgagagggc gaagcaaaat
     8161 gaaaattgat aaaattttga ataacaatgt cgtcatttcg aaaaatggat tcggggaaga
     8221 agtcgtttgt atgggaaaag gactagcttt tcaaaaaaag attggggacg agatttcgcc
     8281 agaagcggtc caaaaagaat ttgttttaag agattctttt gctaaaaacc aattccagca
     8341 gctgatggct gatattccgg cagaggagat cgaatgggtc aaacaagtag tcgaactggc
     8401 tgaagataaa ctaaaagttg aattttcgcc taatatttat ctgacattga ccgatcatct
     8461 tcattatgcg atcacacgag caaaagaaaa tatccaactg cctaatccat tattatttga
     8521 aacaaaaaaa ttttatccga aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa
     8581 acaaaagacg ggtgtggaac tttctgagga tgaagccggt tttattgcct ttcattttgt
     8641 gaatagccaa caaggaaacg aagacatgca agtgacgatg accgctacca caatggtaag
     8701 agatatactg agtatcatca gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa
     8761 ttatcagcgt atcattactc atctgcagtt ttttgcccag cgttatctca aaggggaagg
     8821 atctgacgag caagatgaat ttctctacgc attagttcaa ggaaagtatc caaaagcgtt
     8881 tcattgtgtc gaacggatca atgagtatct tcgacaaacg caaaaacagg aaatggggat
     8941 cgcagagcag atttatttaa cgattcatat tcaaagagta gtaagtgaga aaaaaagcat
     9001 ccaatcataa ggattgttac tgtatggcag gcaaaacctg gattgaaaaa ataggttcac
     9061 gaatgtttgt gactgttttt ttcaatctgg gtttttattt tttaaataaa aaagaaagaa
     9121 ggaaaaaaca atggataatc aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca
     9181 aaagaatgtc aaaagtttag tacattgtgc tacgcggttg cgattcagat taaaagatga
     9241 atctttagca gatacgcaaa agttaaaaga agacccggac gtgatccaag tcgtacaaag
     9301 tggcggtcag tatcaagtag tgattggcag taatgtagca gatgtttatc aatcgatcgt
     9361 ggatgaggaa ggtcttgcag tttcggagga tggaaaagaa gagtccaaaa atattttgaa
     9421 tcgtctgatc gatattatct ctagtatatt tacacctttt ctaggggcga tggcagccgc
     9481 cggggtattg aaaggattct tatcattggc aacggtgatg ggctggctga cagctgatag
     9541 cggtacttat caaatattgt ttgctgcagc agatggtgtc tttaccttct tacctgtcat
     9601 gcttgctttt acagcagcta aaaagttcaa agccaaccag tttttagcag tagcaattgc
     9661 tatggcactt gtgtatccag ctatcacagc agttgctggt gcgggagaag ctatcagttt
     9721 ctttgggatc ccagtgattc tttcaccatc tggttatact tcttctgtca taccaatcat
     9781 tctagccgta tgggttcaaa gtaaattaga gccattcgta aaaaaagtga ttccgcaatt
     9841 tctgcaaatg attttagttc cgcttgtcgt tttagtagtg atggttccat taacatttct
     9901 tgctttagga ccaatcggga cggttgccgg taatgcttta ggcggtctct tcaactcgat
     9961 ttatggattt agtccaatcg tagcaggtct aatcatggga agtttatggc aagtattcgt
    10021 gatgttcggc atgcattggg gattcgtacc gatcatgttt ttgaatatcg aacaatatgg
    10081 gtttgatgta ttgatgccaa tgcttcttcc agccatcttg gcacaaggtg gggctgcttt
    10141 agctgtagca ttgcgaacaa aagatacgaa acttcgagca ttagggattt cttctacagt
    10201 gacttcgtta tttggaatca ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa
    10261 accattcatt gccgcttgta tttctggagg tattggtgga gctattatcg gattttccgg
    10321 agtaaaagct ttctccagca gtttggtcag cttattgacg attccgactt tcattaatac
    10381 ggtggatggt gtagagtcaa atgtgacagt agcagtaata gctacaggaa ttgcttttgt
    10441 tcttgcattc gtaggtacat tgattttagg atttgacgaa caaacacaag acaatcaatt
    10501 agaaaacaaa catgcgaatg caggagaacc gattacttca gcacggcata ccttaaaaag
    10561 tcctttaacg ggtaaagtac tgccattgag tgaagtacca gaccaagttt tttcttctgg
    10621 cgtgatggga aaaggtatcg ctattgatcc agaagtagga gaactagttg cacctgcaga
    10681 cggagagatc acgactattt ttccaactgg acatgctgta gggatcacaa cgactgacgg
    10741 agcggagatt ttgattcata tcgggatgga tacagtagaa ttaaatggga atggctttga
    10801 gatcttagtc aaacaagggg atctcgtcaa agcaggagat ttattgattc gttttgacat
    10861 cgaagcaatt agagcagctg gctatagcgt aatcactcca gttgtcatta cgaatactga
    10921 tgcatttgcc gatatacttg aattagacca aaaagagatt attgcaaacg aagatgttct
    10981 ggcaattgta aaataaggag gaaatcagga tgcaatcacg tttttcagaa aaatttttat
    11041 ggggtggagc aactgctgcc aatcaattag aaggggctta tgatcgagac ggaaaagggc
    11101 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
    11161 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaattgatc
    11221 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
    11281 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
    11341 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
    11401 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    11461 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    11521 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    11581 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    11641 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    11701 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    11761 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    11821 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    11881 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    11941 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    12001 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    12061 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    12121 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    12181 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    12241 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatggatc
    12301 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    12361 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    12421 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    12481 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    12541 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    12601 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttac cgacagaaag
    12661 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    12721 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    12781 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    12841 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    12901 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    12961 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    13021 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    13081 ttctgatgat ttctcggcag tatttttaac agaagaagaa caagaaattc tgaaaacaga
    13141 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    13201 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    13261 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    13321 aaatagcttc tcatattagc ttctcatatt tttcgtttgg ttaggcttat taccgcagat
    13381 acccggctgt ttctggatta ccatttattc tggataaggg ggtgtggcac gattttcaga
    13441 aggaatctat gaaccaatca ctgtattgat tttagcttcg atggtaaccc cattcggctt
    13501 gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat atattcaatc gtcaagaaat
    13561 cgatacattg aagaccgctt ttcctatggg gatctgtcag atcacagagg gatgtatccc
    13621 tatcgtattg aacgacttgg cacgcaatgt catagcaacg ggagtcggtg gggcagtcgg
    13681 cggtggattg agcatgtttt ggggcgcaga tagccgtatt ccggcatcgg gaatgtttgc
    13741 agtcgctacg atgaccagac catgggcatt cattggtgca ttgttagctg gttcttttgc
    13801 tacgggaata actattttat tattgaaaaa accagttgac ccaaatgcag aagtcatcca
    13861 agaagaaaaa gaggaagaag agatatcttg ggatgacttg acaatcagtt aggaggaaca
    13921 gaatggatta caagaaaaga gtacaacaaa tgttcctaga ttcaggagta gtattgaaag
    13981 aaacagaatt agaaagaatc gaatatacag attttggatt aggagcaatc gagactgaag
    14041 gattgaattt atttgtttat gaaaataatg agcgatactg tgggaaagaa ctggtattat
    14101 tgccttatca aacttgtcca gagcatcggc acccacctcg agatgagaat gaaggaaaaa
    14161 aggaaacctt tcgttgtcga aaaggcttag tatatcttta tgtcgaagga aaagaaacaa
    14221 aaaaactttc ctgtattatt ccaaaggggc aagaacgtta ctatacggca aaaaaagaaa
    14281 tcatacttca cgctggagat cagtacacga tcgaacaaaa tattaaacac tggttccagg
    14341 caggtgcaac aggcgcggtt atcaccgaat tttcttccag cagtgatgat ccaagtgata
    14401 gttttacgaa tccagctatt tatcgttaat acaaaggttt gggaacaagc tgatgaacca
    14461 aaatgatcaa atttcgatta tttggttcgt cggctttttt atttagagaa acatcaggcg
    14521 ttggtgaaac ataacacgag aataaaatgg atttttcatt aataggagaa acaagtgtgt
    14581 gactatttaa ataatgcgtg ttattgacat gcaggacgaa atcccaattt cttctataag
    14641 taagtcgttt caattttata tttactagtt attta
//
LOCUS       DAEGIQ010000066        14573 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000066 DAEGIQ010000000
VERSION     DAEGIQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.067"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..572)
                     /locus_tag="KMT45_001821"
     CDS             complement(<1..572)
                     /locus_tag="KMT45_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBE7005282.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGT"
     gene            complement(820..1656)
                     /locus_tag="KMT45_001822"
     CDS             complement(820..1656)
                     /locus_tag="KMT45_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBE7005283.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1667..2596)
                     /locus_tag="KMT45_001823"
     CDS             complement(1667..2596)
                     /locus_tag="KMT45_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBE7005284.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2788..3141
                     /locus_tag="KMT45_001824"
     CDS             2788..3141
                     /locus_tag="KMT45_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBE7005285.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3215..3871)
                     /locus_tag="KMT45_001825"
     CDS             complement(3215..3871)
                     /locus_tag="KMT45_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBE7005286.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4097..6031
                     /locus_tag="KMT45_001826"
     CDS             4097..6031
                     /locus_tag="KMT45_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBE7005287.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6117..7340)
                     /locus_tag="KMT45_001827"
     CDS             complement(6117..7340)
                     /locus_tag="KMT45_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBE7005288.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7334..9232)
                     /locus_tag="KMT45_001828"
     CDS             complement(7334..9232)
                     /locus_tag="KMT45_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBE7005289.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9226..10344)
                     /locus_tag="KMT45_001829"
     CDS             complement(9226..10344)
                     /locus_tag="KMT45_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBE7005290.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10427..11260)
                     /locus_tag="KMT45_001830"
     CDS             complement(10427..11260)
                     /locus_tag="KMT45_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7005291.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11253..12170)
                     /locus_tag="KMT45_001831"
     CDS             complement(11253..12170)
                     /locus_tag="KMT45_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7005292.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12397..13521)
                     /locus_tag="KMT45_001832"
     CDS             complement(12397..13521)
                     /locus_tag="KMT45_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBE7005293.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13704..>14573
                     /locus_tag="KMT45_001833"
     CDS             13704..>14573
                     /locus_tag="KMT45_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005294.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYT"
BASE COUNT         4604 a         2883 c         2349 g         4737 t
ORIGIN      
        1 attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt atttttataa
       61 cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc gctattacca
      121 taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt ttctttttct
      181 ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc ttttatcaaa
      241 taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg atgagacgtt
      301 agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag ttcgataccg
      361 ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata gatttcatta
      421 tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc aaatttccaa
      481 ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc aaaaaccaaa
      541 ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg atattttact
      601 atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg taattctctt
      661 cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg ctgcttctaa
      721 aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg acgtccttgc
      781 gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac gatacacata
      841 ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg ggatttcacg
      901 cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc attcatcatt
      961 tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg aatctgccac
     1021 acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt tcggtgtttg
     1081 gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt aattataaat
     1141 tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct tatccccata
     1201 cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat aaatactggt
     1261 atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt tacttactgg
     1321 gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg gaaacaacgt
     1381 ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat aaggttttga
     1441 caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt ttttgaccca
     1501 caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa tagctaagta
     1561 gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt acatcatcaa
     1621 ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa ataaataatg
     1681 tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag cgtcctgtaa
     1741 aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag accaaaaagg
     1801 cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac gcatctgctt
     1861 ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct aaaatctttg
     1921 ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca gctgcgatta
     1981 atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa tgtgtcaaca
     2041 catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt tgttcttctt
     2101 catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct ttcaatacag
     2161 cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa aagctgccta
     2221 tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca
     2281 cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga actggtacgc
     2341 cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa
     2401 atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc catctcttca
     2461 cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct aataatacga
     2521 aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa accgaatttg
     2581 gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct ttgcaaatat
     2641 acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc ttatttttta
     2701 ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct catacatatc
     2761 acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac tgaagttcaa
     2821 gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt
     2881 tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat tccggcactt
     2941 catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct gactgcgcca
     3001 gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt catcgacttt
     3061 aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct ttcttatgaa
     3121 gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa
     3181 gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt tcggaatgcg
     3241 catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc aaatcaacat
     3301 tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc aagattcctt
     3361 tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg ggtatcgtta
     3421 aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc attggataaa
     3481 cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa gcacaactga
     3541 tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc aagttcccta
     3601 caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca
     3661 taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa taagaaaaat
     3721 ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt gaagacactt
     3781 ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc agtcttttag
     3841 ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt gtctaattac
     3901 atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca ttgctcagat
     3961 attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc gcagtataat
     4021 ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga attcacaaag
     4081 tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc attttggtgc
     4141 agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg tcgctttagt
     4201 tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa ttgaagcacc
     4261 tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg ctcaggacac
     4321 aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg aacaagttag
     4381 acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta cgccagacac
     4441 acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt tttcagatcg
     4501 aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta aattcgattc
     4561 ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac gtttagcttt
     4621 agctcgcatg cttttgcaac accctgacat tttgattcta gacgaaccaa cgaaccattt
     4681 agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg gtgcattatt
     4741 gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct atgaattaag
     4801 cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga
     4861 acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc ataaattaga
     4921 agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag
     4981 aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa
     5041 ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc
     5101 tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc gtcgtcaaga
     5161 agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga aatcaatcat
     5221 tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca ccatcggcta
     5281 ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag aattgtggga
     5341 tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt tcttattttc
     5401 tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag cacgggttgc
     5461 tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac caacaaacca
     5521 tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg acggcacact
     5581 gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag tcgtcgaact
     5641 ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag agaaaaagca
     5701 ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg ctcctgctcc
     5761 cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc gtagccttca
     5821 acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt
     5881 ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca atgagctgaa
     5941 ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat gggaagaact
     6001 aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa accattcatg
     6061 ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta cctttttcac
     6121 caataagaag accaattact agtcattctc actaaagctt ctaaataata gtaatcaccc
     6181 caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga ctgtgttaaa
     6241 aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat aattttccaa
     6301 acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc tattaattct
     6361 aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct gctagaatca
     6421 tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat aaaataattg
     6481 gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata acttaatgca
     6541 aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact ataaccttga
     6601 tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt atgataagtt
     6661 gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg agcagctttt
     6721 ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag attcatgcaa
     6781 caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca agcttgtata
     6841 actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat tgaagcttcc
     6901 ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga atacaaaaaa
     6961 ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa atctatatct
     7021 gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa caaccaatta
     7081 attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata acgtcctttt
     7141 ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat taacctctcc
     7201 gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa ttctttataa
     7261 cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt atcaagcatt
     7321 tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt tggatagtta
     7381 gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct aaactataat
     7441 cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt ccaaatgtac
     7501 cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg ggcaaaccca
     7561 taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata cctgaaaagc
     7621 cagcttcact caccctatag ttttccttgt cttcttctat gattattcct tctttctcag
     7681 gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct aattctactt
     7741 tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt tttgcctgta
     7801 aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct cgtacattat
     7861 ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg gtcgtgatat
     7921 atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct ttcaattgat
     7981 tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa tatttttcat
     8041 tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat gtatgtccat
     8101 tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca cgaataggca
     8161 taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac aataaaaaaa
     8221 tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat gtcaattgag
     8281 ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt tgcatccacc
     8341 atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc aaaagatcgg
     8401 cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg cttcgaccaa
     8461 aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa aaatcaatag
     8521 ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta taaatcaaac
     8581 cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa ctgtctcgat
     8641 accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc agtgctgatt
     8701 ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta aaaaattgcc
     8761 aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata atctggcttt
     8821 tttctcctgt atctaatttt ttccataata attgttcatg atgatataac gctaataaca
     8881 ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa taatcgggat
     8941 gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat cgttcatcta
     9001 attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc attcctgcaa
     9061 ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca cttgctcgat
     9121 aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc tgtctggtcc
     9181 ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc aaatagctaa
     9241 ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat agatcagtgg
     9301 cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga tcccatcttc
     9361 atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga tagcagcttc
     9421 atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa gaccgcatgc
     9481 agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat caatgagagg
     9541 aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt ctgctatagc
     9601 tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata aagcccatgc
     9661 atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta accgaatata
     9721 tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt tcctcactag
     9781 atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg gatcaccagt
     9841 ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa tcatccaacc
     9901 aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc gtccagcaag
     9961 taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact gttgataatg
    10021 tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg aaaaatggta
    10081 cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact tttctcgttt
    10141 tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc accagtcgat
    10201 tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg gaattttatt
    10261 ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa tttttcttga
    10321 ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt tatgaatgtg
    10381 gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac ttatttgcag
    10441 ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag accattggga
    10501 tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca cctgtactag
    10561 aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct gaaagcatga
    10621 ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca ctagtaataa
    10681 tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca ttggcaccat
    10741 cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt cgcatcataa
    10801 acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta ttcgataatc
    10861 ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc accatcctag
    10921 atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt gctaatgcat
    10981 aacccgccat tgtagaactg gctgtagctg aaactgtact gattacagca ataacaatcg
    11041 aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag ttttttaata
    11101 gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta ctttgcgttg
    11161 aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac acaataatga
    11221 cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc tccgaattta
    11281 aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat agcagaggct
    11341 tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac gaccaaagct
    11401 gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa attacccaag
    11461 ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat ggggatcgtt
    11521 aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc gtatatctct
    11581 ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac actaagccaa
    11641 atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca ctgtacaccg
    11701 ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa aaaagtcatg
    11761 aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac agttctaaaa
    11821 aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat agctaatagc
    11881 atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga tttaataaac
    11941 aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa tgagtagcca
    12001 attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt cacaataaag
    12061 aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt atctgttgct
    12121 tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat cgttaagtcc
    12181 tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa aattttctgc
    12241 ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg cagcggtagt
    12301 caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg tattttcatt
    12361 gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact ttttctcagc
    12421 tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca gtgcgttggc
    12481 taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt gtggattttt
    12541 ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc ctggattttc
    12601 ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag gaaaattcaa
    12661 agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt gaaatgcttt
    12721 ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt ctttgattaa
    12781 ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt catcaagcaa
    12841 agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag attctggcaa
    12901 ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga tatattcttc
    12961 ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg gttgataata
    13021 acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac tcgtttgttg
    13081 acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat aaaatccata
    13141 ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg ctgaagtgtc
    13201 tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg tgatcatttg
    13261 tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg ctccagtttt
    13321 gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt ttgcgacata
    13381 tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg ttacttcttc
    13441 gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca aagctatcac
    13501 caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt atataaaacg
    13561 ctttcactta tgtatattaa cggaattagt aacacaaata tttatataaa aaaatatttt
    13621 acttgtcgtt ttggcttttc atttttgtta taatagtcat taattataaa tagaaagctt
    13681 tttatataaa aagagaaagg aaagtggttc ttttgttaac aaacaaccaa tcctccaatc
    13741 ttgtcaaaaa aacactaacc tattccatag atacccatct tcatttatct gctgctccct
    13801 attttcgact actaataaat gaaagcgcta atttatatgc tgaatttgca cataaatcta
    13861 tagaactaga taatccttcc cttttattca ttgataaaca gcaagaagtc caaatctttt
    13921 ctactcaaaa ggaaacacaa acttttatca ttatccagtt ttattttgat gagcaattat
    13981 tagcacatat tacgggacaa tctttttcta tagatcaaga aataacatct atcattactt
    14041 ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca tagtaagcca gctatttcct
    14101 atgatcagga aatcaattct tttttcatac tatgccaaca gatgagcatt catttgcttt
    14161 ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc ttccttacca aggcattatc
    14221 aacaggaact tgctttattg acaatacctt caaatgagaa agtcattcct aaaaaaaagc
    14281 atattcttta ttcagatgaa ttagttgatc caatcatacg atatttagaa gaacatcttt
    14341 cagaaaattt tttgcttgaa caattagccc aaattttttt tatcagtcca gccagcttaa
    14401 aaaaacattt tagaaaagtg aatggaaaaa gtgttattag ctattttaaa gaactaaaaa
    14461 tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac ctatacagaa attgcagata
    14521 tattaggatt tcattcgatc catcattttt cttcagcttt taaaaaatat aca
//
LOCUS       DAEGIQ010000067        14383 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000067 DAEGIQ010000000
VERSION     DAEGIQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.068"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            38..1777
                     /gene="yfcC"
                     /locus_tag="KMT45_001834"
     CDS             38..1777
                     /gene="yfcC"
                     /locus_tag="KMT45_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBE7005295.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1934..3118
                     /gene="iadA"
                     /locus_tag="KMT45_001835"
     CDS             1934..3118
                     /gene="iadA"
                     /locus_tag="KMT45_001835"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBE7005296.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3229..3432
                     /locus_tag="KMT45_001836"
     CDS             3229..3432
                     /locus_tag="KMT45_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBE7005297.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3533..4195)
                     /locus_tag="KMT45_001837"
     CDS             complement(3533..4195)
                     /locus_tag="KMT45_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005298.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4208..5710)
                     /locus_tag="KMT45_001838"
     CDS             complement(4208..5710)
                     /locus_tag="KMT45_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7005299.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6258..7937
                     /locus_tag="KMT45_001839"
     CDS             6258..7937
                     /locus_tag="KMT45_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBE7005300.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8064..8543
                     /locus_tag="KMT45_001840"
     CDS             8064..8543
                     /locus_tag="KMT45_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBE7005301.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8762..9442
                     /locus_tag="KMT45_001841"
     CDS             8762..9442
                     /locus_tag="KMT45_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE7005302.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9450..10781
                     /locus_tag="KMT45_001842"
     CDS             9450..10781
                     /locus_tag="KMT45_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBE7005303.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10796..11758
                     /locus_tag="KMT45_001843"
     CDS             10796..11758
                     /locus_tag="KMT45_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBE7005304.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11869..12207
                     /locus_tag="KMT45_001844"
     CDS             11869..12207
                     /locus_tag="KMT45_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBE7005305.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12286..12993
                     /locus_tag="KMT45_001845"
     CDS             12286..12993
                     /locus_tag="KMT45_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBE7005306.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13096..>14383)
                     /locus_tag="KMT45_001846"
     CDS             complement(13096..>14383)
                     /locus_tag="KMT45_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005307.1"
                     /translation="EIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLINYPQTAV
                     PKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFL
                     SDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKT
                     SSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQ
                     EIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESS
                     SSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTET
                     KEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETE
                     TFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4570 a         2496 c         3115 g         4202 t
ORIGIN      
        1 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
       61 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      121 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      181 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      241 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      301 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      361 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      421 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      481 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      541 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      601 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      661 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      721 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      781 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      841 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
      901 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
      961 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1021 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1081 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1141 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1201 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1261 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1321 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1381 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1441 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1501 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1561 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1621 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1681 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1741 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1801 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1861 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     1921 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     1981 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2041 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2101 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2161 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2221 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2281 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2341 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2401 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2461 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2521 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2581 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2641 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2701 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2761 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2821 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     2881 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     2941 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3001 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3061 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3121 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3181 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3241 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3301 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3361 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3421 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3481 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3541 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3601 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3661 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3721 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3781 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3841 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     3901 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     3961 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4021 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4081 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4141 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4201 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4261 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4321 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4381 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4441 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4501 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4561 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4621 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4681 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4741 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4801 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4861 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     4921 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     4981 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5041 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5101 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5161 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5221 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5281 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5341 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5401 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5461 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5521 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5581 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5641 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5701 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5761 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5821 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     5881 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     5941 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6001 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6061 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6121 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6181 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6241 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6301 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6361 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6421 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6481 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6541 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6601 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6661 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6721 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6781 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6841 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     6901 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     6961 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7021 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7081 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7141 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7201 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7261 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7321 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7381 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7441 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7501 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7561 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7621 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7681 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7741 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7801 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7861 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     7921 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     7981 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8041 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8101 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8161 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8221 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8281 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8341 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8401 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8461 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8521 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8581 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8641 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8701 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8761 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8821 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     8881 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     8941 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9001 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9061 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9121 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9181 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9241 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9301 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9361 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9421 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9481 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9541 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9601 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9661 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9721 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9781 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9841 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
     9901 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
     9961 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10021 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10081 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10141 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10201 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10261 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10321 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10381 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10441 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10501 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10561 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10621 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10681 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10741 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10801 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10861 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    10921 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    10981 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11041 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11101 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11161 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11221 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11281 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11341 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11401 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11461 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11521 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11581 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11641 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11701 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11761 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11821 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    11881 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    11941 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12001 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12061 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12121 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12181 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12241 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12301 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12361 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12421 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12481 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12541 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12601 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12661 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12721 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12781 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12841 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    12901 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    12961 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13021 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagaggat
    13081 gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt tcatatacag
    13141 ttcatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg tatccagata
    13201 gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca aatcagcatg
    13261 ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca aatggctttg
    13321 aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta actgctcttt
    13381 cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga tttggtttct
    13441 ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt cctgtataaa
    13501 agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac tactgctgga
    13561 ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat aaattggtat
    13621 tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca attgaccgac
    13681 ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct gtttttgtcg
    13741 aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg ctctctctgt
    13801 ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg aataatcatg
    13861 tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg gaaaatatgc
    13921 ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt tttcttgttt
    13981 tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta attcaagtga
    14041 ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa gatagagacg
    14101 gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga tccgtaaatg
    14161 gaattttttt aaggaatgat tgagctgttt tgtcagtttt tcgagatagg agggcgatat
    14221 ataaagttgt tcaagtaaat atttttttgg tacagcagtt tgtggataat tgattagtaa
    14281 catcaaatag ttaaaacgat tggattcttt taggtagcat aattttaact tagaaaaaac
    14341 agctaacttt ttagtttgat cggtttcttt ggtttggatc tct
//
LOCUS       DAEGIQ010000068        14166 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000068 DAEGIQ010000000
VERSION     DAEGIQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.069"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            131..1327
                     /locus_tag="KMT45_001847"
     CDS             131..1327
                     /locus_tag="KMT45_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBE7005308.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(1389..1574)
                     /locus_tag="KMT45_001848"
     CDS             complement(1389..1574)
                     /locus_tag="KMT45_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBE7005309.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            1805..5119
                     /gene="dnaE"
                     /locus_tag="KMT45_001849"
     CDS             1805..5119
                     /gene="dnaE"
                     /locus_tag="KMT45_001849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBE7005310.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            5287..6249
                     /gene="pfkA"
                     /locus_tag="KMT45_001850"
     CDS             5287..6249
                     /gene="pfkA"
                     /locus_tag="KMT45_001850"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBE7005311.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            6323..8107
                     /gene="pyk"
                     /locus_tag="KMT45_001851"
     CDS             6323..8107
                     /gene="pyk"
                     /locus_tag="KMT45_001851"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBE7005312.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8142..9044)
                     /locus_tag="KMT45_001852"
     CDS             complement(8142..9044)
                     /locus_tag="KMT45_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE7005313.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            9230..9799
                     /locus_tag="KMT45_001853"
     CDS             9230..9799
                     /locus_tag="KMT45_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBE7005314.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            9857..10036
                     /gene="rpmF"
                     /locus_tag="KMT45_001854"
     CDS             9857..10036
                     /gene="rpmF"
                     /locus_tag="KMT45_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBE7005315.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            10206..11627
                     /gene="gndA"
                     /locus_tag="KMT45_001855"
     CDS             10206..11627
                     /gene="gndA"
                     /locus_tag="KMT45_001855"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBE7005316.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            11820..12506
                     /locus_tag="KMT45_001856"
     CDS             11820..12506
                     /locus_tag="KMT45_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7005317.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            12503..14008
                     /locus_tag="KMT45_001857"
     CDS             12503..14008
                     /locus_tag="KMT45_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBE7005318.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         4653 a         2470 c         3002 g         4041 t
ORIGIN      
        1 tttttgtttt acataaaaag aacgttacat ataaaatgac ctttgcggga tttttctctt
       61 tttactggcg agatcgttgc ttgtttattc gaaaataggt atgataaaag agacgaaaga
      121 gggatgatag atggagatta attggagaaa aaatttagtg attgcgtgga ttggctgttt
      181 tttcactggt gcaagtatta gtttagttat gccattcata ccagtttatg ttgagcaact
      241 aggaacacca aaagatcaaa tcgaattgtt ttcgggacta gcaatctctg tgactgcttt
      301 tgcatctgcc gtggttgcac caatctgggg aaatctagct gatcgaaaag gccgaaaact
      361 catgatgatc cgtgcagcag cagggatgac attaacaatg ggatctttgg cttttgtacc
      421 aaacgcgtat tggcttttga tcatgcggtt ttggaatggg attttgtctg gatatattcc
      481 aaatgccaca gcgatgattg cttcacaggc acctagagaa aagagcggat gggcgttagg
      541 aacattgtct accggagcga ttgctggaaa tctgatcgga ccttcaatgg gcggtgcact
      601 ggctcagtgg tttggtatgg aaaatgtatt cttgatcaca ggggctttgt tgatgatcac
      661 aacggtattg accattttct tagtcaaaga agatttccat ccaatcgaga aaaaagatct
      721 gataagcacc aaagaaattt tttcaaaaat ggatcatctc tctattttga tcggtctctt
      781 cattacgacc ttgattttac agataggaat cactacgatc agccctattt tgaccttata
      841 tattcgtgaa ttgagcggaa gtacagaaaa tatccttttt gtcagcggct tgattgtttc
      901 gatagctggt gtttccgctg tcttttcttc accgaaacta ggaaaactag gggataagat
      961 tgggaatcaa aaagttctaa ttggaggact cattttatca ttttgctgtt atttacctat
     1021 ggcattcgtc actacaccgc tccaattagg tatacttcgc tttattttag gcttttcaac
     1081 tggcgctttg atgccttctg tcaatacact gatcagtaag attacgcctc aagaaggagt
     1141 cagccgaatc tatagctaca atcagatgtt tagcaacttt ggacaagttt taggtcccat
     1201 gctaggatcg acagtcgcgc atgcttataa ttacagtgct gtttttattg tgacaagttt
     1261 gtttgttctg tcaaatattc ttctttcttt attcaacttt cgtaaagttc ttcgcagcag
     1321 attataatgg tggtgaggag tagaaaataa aaaagagctt gagaaatctc aagctcttgg
     1381 ctgttttttt acgaatgggc agaggtttct tttcttggcg caactgcacc aatcaaaagg
     1441 ataatgacac ccgcaatgaa tgaaatgatt gttgtcaaag taaaatcata tgttccgctt
     1501 gttaatgcag caccaatata accgactact tgacctagaa cgacagccca gaataatgta
     1561 acgatatatc gcataaagaa cacctctttt ctatcttaat tttaccagat tttctttgaa
     1621 tgtaaagaaa cgagaccaaa tctcaataaa atttgagttc ttgcgaaaga actcattcct
     1681 acagattaca agcaagcaaa tgaagtggaa taatcaactt cagcgcctgc tatcttaatg
     1741 aaagtaaaag gatcaaacct tttaacgcta gtgaatttta acgaaaaaaa aggcggaatg
     1801 caagatgttt ttcccacaac tttatacaat tacttcttat tctcttttac aaagtacgat
     1861 acgtattcga gaatatgtac aagaagcaaa aaaacaaggc tacacttcat tagccataac
     1921 ggataaagat gtcttatacg gtgcacctga attctatcgt atttgtgtag aagaaggagt
     1981 acagccaatc attggattac atcttagtta tacttatgaa gaaaaaaatt atggcttgct
     2041 tgcgtacgca aaagatgaaa taggttatca gcagctgatg aaactttcca gccgaaaaat
     2101 gatccaagaa aaattggaac ttgcagaaat gacagaaacc gaccatctag cgtttgttgt
     2161 tccggcaaat cattctgttt ctgaggcttt caataaagag gataactttt tcggtcattg
     2221 gcagttttta cttgaaaatg tttttactag aaatttttat atcggcttga cagaaaacac
     2281 gcctgatcaa ttgcctgaat ggtatcagta tgtgataaaa gaaaattatt cgctttgtgc
     2341 attacatccc atcgctagtt tacaacaaga agaacttttt gcacttgaag tgatgaaaca
     2401 tatcaaagag ggaagccaaa tacctttaga aaatttatct gcatctttac cactaacagc
     2461 aaatgtctat cttctttcag aacaagaaat gatcaaacga ttcgaaaaag cgggtctttc
     2521 ccaagctgta ataggcgccc agcgtttagc tgaatcgatt tcatttgaat tccagttcca
     2581 tcagaaatta cttccgcatt atccagttcc taatggaaaa caagctggag aatatttaag
     2641 agagttatgc tattcgcaag tggaacgccg aatcactgac tatacaaagg aataccaaga
     2701 acgtttggac tacgagttat ctgtcatcca tcaaatggga ttcgatgatt atttcttgat
     2761 cgtatgggat gtaatggctt ttgctcatag aaacaaaatc gttaccggtg caggtcgtgg
     2821 ttctgctgcg ggttcattag tagcctatgt gttagaaatc acagatgtgg atccattaga
     2881 atatgatttg ctgttcgaac gctttttaaa ccccgaacgc tttacgatgc cagatatcga
     2941 tttggatatt cctgacaatc gacgtgaaga agtattgaat tatgttcgag aaaagtatgg
     3001 acaatatcat atggcacaaa tcgcaacatt tggcacaatg gcagcaaaaa tggttcttag
     3061 agatgcagct agagtgtttg gtttatcgca aagtgaagca aaccgctggt caaaagcaat
     3121 acccaatcaa ttgaaaatca cattgaccca agcttataaa acttcgcatc aattagtaga
     3181 attagttcag gcaagcgaaa aaaaccaact tttattcaaa actgcagtga ttttagaagg
     3241 attgccaaga catgtttcga ctcacgcagc aggtgtagtt atcagcgata agaatctact
     3301 tgaactagtt ccgctccaac gcggttctga tgaagctttt ctgacccagt atacgatgaa
     3361 tgaggtagaa gcaatcggtc ttttgaaaat ggacttttta ggactacgca acttatcgat
     3421 tattgatttt actttgaaag ctgttaagcg agtagaaggg tatgagatcc gattaaaaga
     3481 gatcccgcta aatgatgcaa aaacgctgct cttgtttcaa cgaggcgaaa cctcaggagt
     3541 atttcaattt gaatctgctg gtatccggaa cgttttacga agattagatc cagaaaacat
     3601 cgaagatatt gccgcagtca atgcattgta tcgaccaggc cctatgcaaa atatagatac
     3661 cttcattgcc aggaagaaag gaaaagaagc cattcgctac ccagatgatt cactgatccc
     3721 gattttgaaa aacacatatg gagtgatcgt ctatcaggaa caaatcatgc agatcgcctc
     3781 gactatggct ggatttacat tagggcagtc agatattctg cgacgggcag tgagtaaaaa
     3841 gaaaaaagac gtattagatg aagaaaagtc gcattttgta tcaggagcat tacaacaggg
     3901 atattcggaa aaaacagcag cgaccgtata tgaatatatc gaaaaatttg caaactatgg
     3961 attcaatcgg tcacatgcat ttgcttattc attcgtggga ttccaaatgg cgtatctaaa
     4021 agcccatcat ccaggtgctt tctttaccag cctgatgaat tctgttcggc ataacacatc
     4081 aaaattgaaa gaatatattg cagaagcacg taaaaataaa ctcaaacttt cctcgccgtc
     4141 cattaatcaa agtaattcca gttttgagtt ggtcaataag gaagaaatca gattcggact
     4201 gacagcgata aaaggtattc ggcgtgattt tgtagaagat atattaaacg aaagaaaaca
     4261 aaatggcccg ttccagtcag tggatcaatt cctcatacgg atcgataagc gttggctgaa
     4321 attagagtta ctgcaatcac tgacagcagt aggagttttt gatgaattat ctcctaacag
     4381 gaaacaattg atgcttgatc tcgaaggaaa aatccaaaat atcgtttaca gtggtggaag
     4441 tcttgacttg ctgggaatca tggcattaaa agaagaagaa gtcgctgact atacactaga
     4501 agaacgcttg aagctggaag aggagtactt aggtatttat ttgtcaggtc atcctgcaga
     4561 agggtttgat cgtcttcgtt tagctaaaca agttcagctg attacagata tcgtccctaa
     4621 tcaaccagtc aatttattag tgatgatcaa ggaagttaga gaaattcgga caaaaaaagg
     4681 agaactgatg gcctttgttg aaggaaccga cagtagtgga gaaatttctg tcacagtttt
     4741 tccaactctt tatcgtaaat atcgtaattt gtttgccgtt gatcgagtga tctttgttca
     4801 agggcggagc gaaatcagca aatataatca ggaactgcaa ctaattggag aaagctttgc
     4861 tgatcctaac cagttagaag cccagtatcc ttcacaaact tgctatttgc gtattacaga
     4921 agaacgggaa gatcccgaga taatgaaaca attacaagtg atttttcaga aacacccagg
     4981 atatatacca gtagttatgt atcacgaaaa aacgcaacga aaagtagttc tgtcagatgc
     5041 ttattgggtg gatgacagca agagtataaa gcggcaactt atttatctgc ttggcgaaga
     5101 taatgtggtt ttcaaataac ttacggtaaa aaatactact tgctttcatt ttttttcatt
     5161 tttttatccg taaatcaaga caagtgagaa aaataatgtt agaatgttag tcgtggttaa
     5221 cgcaaaaatg gttgtaaacc taagtcatac ttaggataaa gtattttaat gaggtgaatg
     5281 tttattatga aacgcatcgg aattttgacc agtggcggag acgcacctgg aatgaatgca
     5341 gctgttcgtg ccgttgttcg taaaggtatt tacgaaggac tggaagtata cgggatcaat
     5401 tatggttttg ccggtttagt tgctggagat attcgtcgtc ttgatgtggc agatgtaggt
     5461 gacaagatcc aacgtggtgg tacattcttg tattctgcac gttatccaga atttgcaact
     5521 gaagaaggtc aattaaaagg aatcgaacaa ttgaaaaaat tcggaatcga aggtctagtg
     5581 gttatcggag gagacggctc ttatcatggc gcaatggctt tgacaaaacg cggtttccca
     5641 gcagtaggta ttcctggaac aatcgataat gatattcctg ggactgattt cacaatcggt
     5701 tttgatacag caatcaatac agtactagaa tcaattgacc gtattcgtga cacagcgact
     5761 tctcatgttc gtacattcgt tatcgaagta atgggacgca atgcaggaga catcgcttta
     5821 tggtcaggtg tggctggcgg agcggatgaa atcatcatcc cagaacatga ttttgatatg
     5881 gcttcagttg ctaaaaaaat ccaagaaggc cgcgatcgcg ggaagaaaca ttgcttgatc
     5941 atcttggctg aaggtgtaat gggcggaaat gaattcgctg agaaactttc agaatttggt
     6001 gactatcata cacgtgtatc tatcttggga cacgttgtcc gtggtggagc accaagcgca
     6061 cgcgaccgcg tgttggcaag taaattcggt gcttacgctg tcgaattgct tcaagaagga
     6121 aaaggtggat tatgtgtcgg tatccatgac aacgaaatcg ttgcgtctga catcattgat
     6181 acacttgaaa acaacaaaca taaaccagac ttatcactat atgatctaaa taattcactt
     6241 tcattttaag agtgaaagaa accaagctat ttaagctgtg tacagcttta aataaacaaa
     6301 attgaatggg agagtttttt aaatgaaaaa aaccaaaatc gttagtacat taggaccagc
     6361 aagtaactca gtagaaatta tttctcaatt gattgaatct ggggcaaatg tcttccgctt
     6421 taatttttct catggtgacc atgaagaaca attatcacgt atgactatgg tccgtgaagc
     6481 agttaaaaag acaggaaaag atgtcggtat tcttttagat acaaaaggag ctgaaatccg
     6541 tacaactgtt caaggcacaa cagaagctga ttttggtcgt gcaggataca tcaaatttga
     6601 agtaggagat caaacacgca tctctatgga tcctgaacat gtaggttcaa aagaaaaaat
     6661 cgcggttact tacccaggat tatttgacga tgttcacgtt ggcggacaca ttttatttga
     6721 tgatggtttg atcgatatgc aaatcattga aaaagacgaa gcaaaccgtg aattagtcgt
     6781 agaagttaaa aatgcaggta tgctaggatc acgtaaaggg gtaaatgcac caggcgtttc
     6841 tattagtttg ccaggtatta ctccaaaaga tgctgacgat attcgttttg gtttagacaa
     6901 cgatatcgac tttatcgctg caagttttgt acgtaaagca caagacgttc tagatatccg
     6961 cgaaatctta gaagaaaaag acatgactca tgttcaaatc ttccctaaaa tcgaatctca
     7021 agaaggtatc gacaacattg acgaaatcat caaagtttct gacggtgtta tgattgcccg
     7081 cggagatatg ggtgttgaaa tcccagctga actagtacca atggttcaaa aacgcattat
     7141 caaaaaatgt aatgctgctg gtatcccagt tatcactgct acacaaatgt tagaatcaat
     7201 gcaagaaaac ccacgtccaa cacgtgcgga agcttctgac gttgctaacg ctgtatttga
     7261 tggcacagat gcaacaatgc tatctggtga atctgcaaac ggggactacc cagttgaagc
     7321 tgtttcaaca atggcacgta tcgaccaaga agctgaaaat gcattatcag aattaggtac
     7381 attccaattg aacgaattcg ataaaacaga tgtaactgaa acaatcggtt tatctgtagc
     7441 tcgtgctgca aaaaatctag gcgtgaaaac aatcgttgct gcaactgaat caggatacac
     7501 tgcaaaaatg atttcaaaat atcgtccaga tgcagatatc ttggctgtta cttttgacga
     7561 acgcacaaaa cgtggtttga tgttaaactg gggcgtttac ccaacagttg ctgaaaaacc
     7621 atcaacaact gatgaaatgt ttgaactagc agctaaaaaa gctgttgaat taggtttcgc
     7681 atcagaaggc gatttaatct tgatcactgc tggtgtacca gttggtgaac gcggaacaac
     7741 taacgtgatg aaaattcaaa tgattggttc taaattatta gaagctcaag gcgttggaca
     7801 aaaatctgtc gttgcaaatg cagttattgc ttcatctgct gaagaagctg taaaaaaagc
     7861 aaaagacgga atggttctag ttgtaccatc aactgacaaa gaatatatgc ctgcaatcga
     7921 aaaagcagct gctgttatcg ttgaagaagg cggattgact tctcacgctg cagttgtagg
     7981 aattgcacaa gatattcctg taatcgttgg tgctaaaggc gcaacaacaa caattacaga
     8041 aggtgaattg attaccgttg attcacgtcg cggcatcgtt taccgtggcg aaacaacagc
     8101 tatctaattt agcttaataa aaaataaaaa cagctcttct tttacgaaga gctgtttttt
     8161 tgtgctgtaa cgaattcatg gataacttgg actacttgtt cttgaagagg cgtgacgatc
     8221 tgctgtgttc gttttgccaa tgaaatcaag aagcgaggct gaacttcatc tgaaagaggg
     8281 aggcaaacta aatgatcttc aggatggaca gcaatctctg ccaaaaaacc gatacccaca
     8341 ttttctcgga ccatactctt taaaatcgtc aagtcattgc tttgataaac aacttttgga
     8401 tgagtatgtg tttgtttaga aagcttagtg aatgctgcgg ggtggacata atgttcgttc
     8461 aatagtacaa actgttcatc tgctatatct ttgaaagaga cagtatcctg cttggaaaga
     8521 ggatgtgtag gtggcacgac gatcatgaaa cgtttgtcca aaagtaactc tgtttctaaa
     8581 tattgatcca cgattggctg tgttgagcct aatacagcta aatcgatcct cccttgtcgg
     8641 atcatcccgt ataagtcccg agagcctcca tcggtaatct caatttgatt catcaattca
     8701 tgcttgataa agtaacttga cagtttagga aagtattcat tgccgataat aggaggcatg
     8761 ccaaaacgaa gctttttctt ttgcaactga gtgatttcag ctcttgcagt agaaatctct
     8821 tggagaatgg tttgtgcatg gagaaacagc tgctttccag aaggcgtgac gattaattcg
     8881 tgatgcgatc gatctcttgt aataagtacg gtgtcaaact ctttttcaag acgttgtaat
     8941 gcatatgtaa tagtaggttg gctcacacgg aaataattag ccactttcgt aaaatttttt
     9001 tgtttgacga tttggacata gtattcgata tctttcgtgt tcatgaagat catccgctcc
     9061 tttaatggaa taatgattat tgtaaatgat tctgaggatg atagcaaaca gaggctttct
     9121 aataaagatt tttctgtttt tttatgaact gcaaggtgtc aagaaaattt cattgacaac
     9181 atgctttttg ccctgtataa ttgcttttgt tgcttaggag tgatgaaaaa tgaaatggtc
     9241 tttattggaa ttgagaaaat accaggagac accgttaact tttcatgaga cgctcgactt
     9301 gaaagccgct ctcatgaagc gagacaattt aatacttgat gttgcgccag tggaagtcga
     9361 aggtttggtc tcagttgata agaaaggcta tatcgttcat tacactgtgc agacgacttt
     9421 gacagttcct tcaacacgtt cactagaacc tgttgaactg cccatggatt tttctgtaga
     9481 cgaagtattc atgacagaag aacaattcca aagtagagat gaacaaatgt ctgcggatga
     9541 gattttgcta attgaaggtc cattattgaa tttagcagag tcgattgaag acaacatctt
     9601 attggcgatt ccaatgcggg tattgacaaa agaagaggaa gcagcaacag aacttccaaa
     9661 aggcaatgat tgggaagttg tttccgaaga agaataccag cgcagaaaaa tggaagaagc
     9721 cgagaataaa attgatcctc gtcttgcaaa actatctgaa ctttttgatt ctaaatcaaa
     9781 agacgacgat aacaagtaag attggaatat attgtgtcta atttagatac aagactttac
     9841 aaggaggtgt agcaaaatgg cagtaccagc tagaagaaca tcaaaagcta aaaaagcaaa
     9901 acgtcgtaca cactacaaat tgacaatcaa agggttaaac gaatgtccaa actgtggtga
     9961 aatgaaaaaa agccatcacg tatgtgcaaa ttgtggttac tatgatggta aggacgtaat
    10021 gtcaaaagaa gcttaataca ttttaattag aaaatgtaaa acctcaaggc taaccatgga
    10081 gccttgaggt ttattttttt gtagtcggta aataaaaaat atgttgttct ttagtttgca
    10141 atgtagccgt gttaagctat aatgtaagag attagggaga aatcccgtat gtaggaggag
    10201 ttcatatgtc aaaacaacaa atcggcgttg tcggtatggc cgttatgggt aaaaatctag
    10261 ccttgaacat agaaagccgc ggctattcag tggctttatt caatcgtaca ggggctaaaa
    10321 caaccgctgt agtagaagaa cacccagata aaaatttcaa agcaacatat acaatcgaag
    10381 aatttgtaga atctatcgag aaaccacgtc ggatcttgct aatggtcaaa gcaggaccgg
    10441 caacagatgc aacgatccaa gagttgcttc ctcaccttga taaaggcgat attttgattg
    10501 acggaggaaa tacattcttt aaagatacga tgcgtcgtaa tgaagagtta gccaactctg
    10561 gcatcaactt tatcggtaca ggcgtttccg gtggtgaaga aggagcttta aaaggacctt
    10621 caatcatgcc tggtggacaa aaagaagcct atgcgctagt tgcaccaatc ttagagcaaa
    10681 tttctgcaaa agcagaagac gaagcaccat gtgtcacata tattggccca aatggagccg
    10741 gacattatgt gaaaatggta cacaacggta ttgagtatgg agatatgcaa ctgattgctg
    10801 aatcttatga cttaatgaag aatattttaa acctttcagt agaagaaatg gctgatatct
    10861 ttaaagaatg gaatcaaggc gaattagaca gctatttgat agaaatcact gctgacattc
    10921 tgacacgtaa agacgatgaa ggaacaggta aaccagtagt agatgtgatc cttgatgcag
    10981 ctgggaataa aggaacaggt aaatggacaa gccaaagcgc gcttgactta ggtgtgccac
    11041 ttccattgat cactgaatct gtatttgccc gctatatttc tgcttataaa gaagaacgcg
    11101 tacaagcaag caagatttta tctcgtacga acgattttga gttcacggga gacaaaaaag
    11161 aattggtcga aaaaatccga gaagcgttgt atttcagtaa aatcatgagt tatgcccaag
    11221 gatttgctca attacgtgtc gcttcaaaag aatttgattg ggacttgcca tttggtgaaa
    11281 tcgcaaaaat ctggcgcgct ggatgtatca tccgagctcg attcttgcaa aaaattacgg
    11341 atgcctatga caaaaatcct gagatcgaaa atttattgct agatgattac ttcgttgaaa
    11401 tcacgaaaaa atatcagcaa tcagtcagag acgtcgttgc cttggctgtc caagctggcg
    11461 tacctgtacc gactttctca tcagccatcg cttacttcga ttcatacaga gcagaacgtc
    11521 tgcctgcaaa tataatccaa gcccaacgcg attactttgg cgcacatacc tatgaacgtg
    11581 tcgacaaaga aggaatcttc cattactcat ggtaccatga agaataatag tctttcggct
    11641 gttaggaaat gagaaaataa aaagaggacg aaagagctga agtcgattgt tatgagtaga
    11701 cttcagctct tttttaaaat tgctatccat gataaaatga ctatagattt ttaagcatat
    11761 tatcggtata atgatagagt ttttaaaaag ccagtagaat agaaaggata aaataataaa
    11821 tgagcaatat tctaatcatt gaagatgaaa agaacttggc acgatttgtc gagctagagt
    11881 tgaagcatga agggtataac gcagaagtcc actacaacgg ccgaactggt ttagatgcgg
    11941 cattaaataa tgagtgggat gccatattgt tggatctgat gctcccagaa ttgaacggtt
    12001 tagaagtctg cagacgtgta cgccaagtca aaaatacacc aatcattatg atgactgcaa
    12061 gagattctgt gatcgatcgt gtatctggac ttgaccacgg agcagatgac tacatcgtca
    12121 aaccatttgc tatcgaagaa ttattagcac gtcttcgtgc attgttgcgt cgaatcgata
    12181 ttgaaggcga taaaaatgtg gctaaacaaa ctactattac ttatcgcgat ttgactatcg
    12241 aaaaagaaaa ccgtgtcgta cgtcgcggaa atgaagtaat tgaactaaca aaaagagaat
    12301 acgagttgct gcttacttta atggaaaacg tgaacgtggt attagcacgt gacgttttac
    12361 taaataaagt gtggggatac gaaacagaag ttgaaacaaa cgtagtggac gtatatattc
    12421 gttatcttcg caacaaaatt gacgttcccg gggaggaaag ctatatccaa actgtacgcg
    12481 gaacaggtta tgtgatgcgc tcgtgataaa gccgaaaaaa tcggaaaaaa gagaactgaa
    12541 agggccctcc ttgacaatca agtgggcctt tgcgagttct ttcttcatat tcgttgtgtt
    12601 tacaattttc gctgtcatta cctataaatc atctatcaat ctgattgtgg caaaagaaag
    12661 aaacaatgta gagcgtacga tttcagaagt agcttctcgt ttggctaact cggatgaaga
    12721 actgacatta gtaaatacgt atcgtaatct gacgaaagcg acaaatgacg gagatgaggt
    12781 cgtagatcat acttcactgg tcgaaggaaa cttaatgaag atcgattcct ttatagctga
    12841 gctagggcag ccatcaatgg atctttatgt ttatgatttg gacgagaact tgatttttaa
    12901 aacccatgaa aacagtcgaa acctgattca gactacacgt aaagttccaa cgatcgttac
    12961 actagaagga aaaactggct ttttatctat ccagcctata tattctaaag gtacaagaga
    13021 aaagatcggc tatgcgcaaa gcttctatga attatcttct ttctatgata ttcgaaataa
    13081 gctattattg actcttattg ttttagaagt tgtttctttg atcttaagta gtattttagg
    13141 tttcttttta tcttcttatt tcttaaaacc actcaaggta ctcagagata caatggatac
    13201 tatacgtaaa gatccgcaat cagatatcca catgcctgaa atcgacacaa atgatgaatt
    13261 ggcagattta gctgaaatat tcaatgagat gcttgaccgt atgcgtttgt atattgaaca
    13321 acaagagcaa ttcgtggaag acgtatctca tgagttaagg acacctgttg caattattga
    13381 agggcatctt agtctgctaa atcgttgggg gaaagatgat cctgagatat tagaagaatc
    13441 attaagcgcc agcatgcaag aaattagccg tatgaaaagc ttagttcaag agatgctaga
    13501 cttatcaaga gcagaacaag tagatgttca ttatgcgaat gaaacaacta aaggaaaaga
    13561 agtcacctat caagtttata ataatttcaa gatattatat ccagactatg tgatcacttt
    13621 agatgatgat ttgacaaaag aagtgaccct gcaaatttat cgtaatcact ttgaacaatt
    13681 aatcattatc atattggata acgcagtaaa gtattcaact acacgaaaag aagtccatat
    13741 ctctatttct tccactctga atgaatttga gatcgcgatt caggactttg gcgaaggaat
    13801 acccaaagaa gaccttaata agatcttcaa tcgtttttat cgagtggata aagcaagagc
    13861 tagaacgaag ggtggaaatg gtctggggtt gtctatcgct aaacaactgg ttgaaaacta
    13921 taaaggacgt atcttagcag aaagtgtagt agggcaaggg acaattttta gaatctttat
    13981 tcctatcgca aaggcagaag acaagtaaag catgtgcttt acttgttttt ttatatatcc
    14041 aacaaagaat aaggtacaaa atacaagcaa gttagaaatt cagggatgtc attataaaaa
    14101 aaactagcga acaattaaat agaataacat ccgttaatta aacgtaagta gcttttagat
    14161 gaacga
//
LOCUS       DAEGIQ010000069        14074 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000069 DAEGIQ010000000
VERSION     DAEGIQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.070"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..353
                     /locus_tag="KMT45_001858"
     CDS             <1..353
                     /locus_tag="KMT45_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBE7005319.1"
                     /translation="IFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIA
                     KQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFN
                     ILRRNPKELLIDNN"
     gene            complement(639..2261)
                     /locus_tag="KMT45_001859"
     CDS             complement(639..2261)
                     /locus_tag="KMT45_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HBE7005320.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            2913..3884
                     /locus_tag="KMT45_001860"
     CDS             2913..3884
                     /locus_tag="KMT45_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBE7005321.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(3962..4198)
                     /locus_tag="KMT45_001861"
     CDS             complement(3962..4198)
                     /locus_tag="KMT45_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE7005322.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(4167..4298)
                     /locus_tag="KMT45_001862"
     CDS             complement(4167..4298)
                     /locus_tag="KMT45_001862"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE7005323.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(4374..4757)
                     /locus_tag="KMT45_001863"
     CDS             complement(4374..4757)
                     /locus_tag="KMT45_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE7005324.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(4790..5575)
                     /locus_tag="KMT45_001864"
     CDS             complement(4790..5575)
                     /locus_tag="KMT45_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBE7005325.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            5753..6853
                     /locus_tag="KMT45_001865"
     CDS             5753..6853
                     /locus_tag="KMT45_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBE7005326.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            6843..7667
                     /locus_tag="KMT45_001866"
     CDS             6843..7667
                     /locus_tag="KMT45_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBE7005327.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            7664..8479
                     /locus_tag="KMT45_001867"
     CDS             7664..8479
                     /locus_tag="KMT45_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBE7005328.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            8476..9306
                     /locus_tag="KMT45_001868"
     CDS             8476..9306
                     /locus_tag="KMT45_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7005329.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            9313..10344
                     /locus_tag="KMT45_001869"
     CDS             9313..10344
                     /locus_tag="KMT45_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005330.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(10451..11724)
                     /locus_tag="KMT45_001870"
                     /pseudo
     CDS             complement(10451..11724)
                     /locus_tag="KMT45_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            12001..12210
                     /locus_tag="KMT45_001871"
     CDS             12001..12210
                     /locus_tag="KMT45_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005331.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            12260..12976
                     /locus_tag="KMT45_001872"
     CDS             12260..12976
                     /locus_tag="KMT45_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBE7005332.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(12878..13398)
                     /locus_tag="KMT45_001873"
                     /pseudo
     CDS             complement(12878..13398)
                     /locus_tag="KMT45_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13686..>14074
                     /locus_tag="KMT45_001874"
     CDS             13686..>14074
                     /locus_tag="KMT45_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7005333.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLS"
BASE COUNT         4058 a         2623 c         3122 g         4271 t
ORIGIN      
        1 aaatttttgg tcaattccta attgagatgg tatttatatc aatccccgca ttcgttggtt
       61 cctatttctt agctcaatat acagctgaca agcttgggaa taatatattg aataaggtta
      121 ctggtgatat agctaaacaa atagctaaac aatctgcatc tagccaatta ggtggtgggg
      181 cagaagctga aggatttaac aagacattat caagtttaga tatcaatgta ttacctaaat
      241 tcatgattta tgtagttata tttatgagtt tagtacttct tgtatcattg attatttctt
      301 cattcaatat attaagaagg aatccaaaag aattattaat tgacaataat taaaattttc
      361 ggtgcttttt agcaccaaaa atgagagtgt aatataaacc gtgtaagtaa ccatcaatcc
      421 taggcgggtg gttgcttcac ggtttttatt tttctaaaat aagatcaaga ggtgattaca
      481 cgatggctag aaagaaaaga aatcctgatg ctgagaagtt agctgaatcc attttgaata
      541 cttatcaacc cgaatctgta gaggatatgc aagatgtttt aaaagatgtg tttggtccct
      601 tatttgaaaa aatgcttcaa ggagaattga ataaccattt agggtatgac gcccattcta
      661 aagagccaaa gaaacatgac aatcgacgaa acggttatgg caataagacg cttaaaacga
      721 gctttggtaa agtaaacatt gatgttccca gagaccggga ggcttctttt gaacctgagt
      781 tgattcctaa tagaaaacga gatgtctccg acatcgcaga ctgtagacaa aagctgttcc
      841 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
      901 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
      961 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     1021 atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg
     1081 taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc
     1141 caaacatgtc tcgtgatgac tcgttgatga tttttactta tggtacattg gtttagcaga
     1201 ggacagttca cacattcttt tggattggat ttgtattctc ggtacccttc tcgagtaacc
     1261 gttgaaaaag cgagcatttg atcttgcgga caaatataac aatcaaaata ctcgtcatag
     1321 agaaactcat tttttgataa tagctccttt ttccctttgg gccttgaata aggcaaacaa
     1381 ggtcgaaggt tcaaatgagt caaaaagtga acaaacttag gtgtcttata cccagcatca
     1441 gcgacaacac aattgatttc aggaaaaagt ctcttcgatt gtttgacgag ctgaaaggcg
     1501 acttgactgt catgaatatt tctaggagtg atgattgtgc taagaataaa tccattgtcg
     1561 tcgcatgaag tatggacaga ataagcaaat tgtttttctc gttcgccttt gacgtagtag
     1621 ccgctttccg gatcagcttt acttattttt ctttctttca gcttcacttc cttttctaag
     1681 tccaaagtaa aagggcgttt tccagcctct attcgttgac gattgatctc atcttctaat
     1741 tcatcctgat aggctttcgc ttcatcatgg gtcatttctt tcgtaaactt atgtttattg
     1801 gcgttagctt tgatgtgggt agaatccaga tacaaattat cctctgttac aaaacctgct
     1861 ttaactgctt gttctaaaat ataggcaaaa atatcctcaa acaccgtcgt ttctctaaat
     1921 cggcgaacat aattctttcc aaaagttgag aaagggggga ttttctcctc gaaggagtag
     1981 cctaaaaacc agcggtaagc gacattggta tcgacttcct taatggtttg tcgcatggaa
     2041 cggataccga ataaatactg aataaacacc aatttgatca gaatcactgg atcgatactt
     2101 gggcgaccta aatcggagta agtggcttcg acgattggat aaataaattc aaaatcaagc
     2161 gcggcatcaa tctttcgaac gagatgatct tgaggaacta gatcatcaat cgtaagcatc
     2221 gcaaattgat ttctaccatt cgtattgttt ttagacatca tagctatcac tccttgcatt
     2281 cgttttcata aatctattat aaaagaaaag actgtagaca tcatccataa aatggacttt
     2341 gtctacagtc tggataacgt gattttttgc tgtaatgaca aggttttagc tgtttcagat
     2401 accatgtatc caaaaatagt ttattgagaa aataggagag tatttgatac taactagtag
     2461 gaaaaaatga gataaaaagt aaatggaatc atttaaggta atctgaaata aaaaatatac
     2521 agccctgtgg tactttacct acaaggctgt atatattttt tactctcagc gtttttgact
     2581 ccgtgtctga ctccgtttta ttgattttga atgttattca atgaccattt aacaggtcaa
     2641 atttgatgtt tttgattccg tatgacgccg tttgatatct agtgatatag tgatttgagt
     2701 ctacatcgaa taagggaata aaccaataaa aaatgattta gaatattaaa ataacaaaaa
     2761 agaggactac ctgtattagt cctctttttt gaccagattt tgaccagtct attttacatc
     2821 ctagtatatt tatatgagct aattttatga ttaaatggta agtttttcct tgtgtgtttt
     2881 agtttaaact agtgtgtaat tcaaaaatct atatggaagg aacagtatta agaatagaac
     2941 agggttctct acatgatggt gctggtttac ggaccgttgt ttatttaaag ggctgcccat
     3001 tacgctgtgc ctggtgttcg attcccgaat cacaaagcaa gcagattgaa aaaggatttg
     3061 gccagaccat gaccgctgaa gaggtaatgg atgagattga gaaggatgct gttttttact
     3121 ttcactcgga tggcggagtg accattagcg gaggcgaggc actggttcag gcggattttg
     3181 ctaaagaaat tttgcaaaaa tcgaaataca ttggaatcaa taccgtactg gaaaccagct
     3241 tttgtggtgc ttataatgaa atccaaaagg tagctcccta tgtggataca ttgtttgtgg
     3301 atgtaaaaat gtttaccagc aagctccaca aacaatggac cggcttagat aaccaacaaa
     3361 ttttgaagaa tattcgtcgc tttttaattg agtatcctaa ctgcgaagtg cgaattcgag
     3421 ttcctgtggt tccaggtatc aatatgaacc tgaccgagct gctgacgatt gcttgttttg
     3481 tggcagatct tgatcggttc gtaccgttag aattactacc gtaccactgc tatggtatgc
     3541 acggttacca agcattggga ctggagtatc cgttggcaga cacgccagcg ccttcaccta
     3601 aagaaatgtt tgcattggca gatcaactgg ctcgcacggt tcctcatttg ctggtcacag
     3661 ctttaaccaa aacatttatt tatttattga atccaagctt tatcttatgg ataaatgaaa
     3721 gacactcact agctgcagcc agctcaagaa tagagctttt gctgaagtca gtgagtgtct
     3781 ttttacgatg ttttttcgaa gttaatttgt cgaatgaaca ctggctcaat cggtacatct
     3841 tcactgtttg tcggctgagc tgcgatcgct gctacgattt ctaacccttg gtatacctgt
     3901 ccaaaaacag tataacgacc atccagctcc ggtagtcctc caagctccaa gtatgcttgc
     3961 tttacattag caggtcagcc tgcgcctttg tgttttgcac aataaaaaac tggttgccgt
     4021 tggtgtgggg gccgtggtcc cctaaggcca aggcgccatt aaaatgataa agtccgcgat
     4081 gcacttcatc agcgaaaaag ccctcatagc tggaaacagc ttcatcacca gaaccatcca
     4141 aagcgccacc ttgaataaca aattccttaa tgacacgagc cgaaaggtgt gttgtcataa
     4201 acgccttgct cagctaggga gcgccagttg gctacagcca ggggagcttg gtcaggaaat
     4261 aagcggagac gaatttcacc agcatcggtg accagcacac aaacttcttc ctgctgcggt
     4321 ttttctaatt gaaagagagg aaggtttgca actgaaacgc cttgatcctg ccatcaaata
     4381 tcgaaaatcc gccactcgac cttcgcctga tcaccaaggc gagccattcc caataaaacg
     4441 gtataggcgg tttcatccgc ttgaaaggtc aattgcagct gtcctcgtaa atcgttgtag
     4501 tcatctgggg cggctacttg ataaaaaaga tgaaactgcg catgcaaata ttccaaaggc
     4561 tgatctttaa aggctgcaaa ataagcctga actttttccc gctgcaagct gtcagccacc
     4621 aaaaaatttt ccagttgttg ttttggaacg gctgctaaca aggatcggca aaccgcctca
     4681 attttcaacg catcccaatg tcgcgcaatt aaataatgga tgggtgcctc ttgattgtag
     4741 tcccacaggt tgctcatttt tttcaccact ttcttctatt atataggaat cacaaaatcg
     4801 gcagcggcgt caggcggcgt tcttttcctt gaatgactag caacttttct acctgacagt
     4861 ctgccaacag ctgactcatt tttttaaagc ccagctgata atcttcggca acatgagcat
     4921 ccgagcctaa ggtaaagagc tttccgccga cactttgata caaaggaacg gcataacggt
     4981 aaaggtcttc gttgccgtat ttcaagaagc tcttggcatt taactccatc gccaattctc
     5041 gttcaacaac ctttttgaaa atcgttagca gctgagcctc aaaatgctcc gcgaattctt
     5101 ccgctgtgaa ggtgaagcgg cgtaatccat aatcaaagtg agtcaaaata tctccttctg
     5161 gaaaatccgt caagacctgc aacatctgat caaaatattc cttggtagtt tgaatgggat
     5221 ctttggttaa aaccacatcg tccatataat caaagcggcc attttgatga atacttacca
     5281 gctttaaatc ataaggatgc tgagcgaggt aatccacgat ccgctcttct tgtcctggaa
     5341 caacgccaat ctcgatacct cgcagaaatt tggtggaata ggtttctttt aaagagtcca
     5401 gtttgtttat ataggctgga taatccggaa tgtcatcttt tccggtagat ggattgttta
     5461 aatcaaagtg atccgtagaa acgaaaaact ccggctgata ggctaaatag ttttcaaatt
     5521 tttcctctga atcaaaggaa aaatacgtgt ggagatgctg atcataatac tgcataggga
     5581 gcctcctttt ttctactatc ataccgcaaa agctttagaa ggttaacggg aattcgtcga
     5641 aatttacaaa ccatatacaa cctttacatt gttcatgaaa ccttttcaag aaactgattc
     5701 tctcattaca aagctttgtt agaatggaat tatcgaaagg ggagaagaat gaatgaaaat
     5761 gaatgaaaaa attcgtaaca tgactatcgg tttattttgt gcagcaacag taagtatcct
     5821 gctgacaggc tgcggctcag gtgaagcaac gaagcaatcg gctgcggcag acgactcttt
     5881 agatacgatt atagaaaaag ccaaggaaga aggagagatt gccagcttgg gaatgccgga
     5941 tacttgggca aattgggtag gcacttggaa cgaccttgaa aaagaatacg gcttaagtca
     6001 cgtggatacg gatatgtcca gtgcagaaga attggctaag tttgaatcag aaggaaaaaa
     6061 tggaaccgcc gatattggtg atgtcggtat cagcttcggt ccgttggctg aaagcaaaga
     6121 gttaacctta ccttataaga ccaactattg ggaggaaatt ccggattggg caaaggatga
     6181 tgatggggat tggttgttaa gctatacggg aaccatttcc tttatcaccg ataaagagaa
     6241 tgtgaagcaa gcaccgacta gctggaagga gctgcaagag ggtgactatc aagttagcgt
     6301 gggggatgtg acgattgcca accaagcaca atttgctgta ttggctgcgg cgattgccaa
     6361 cggaggtgac gaaaaggata ttcagccagg tctgaattac tacaaaaaaa tggcagaaaa
     6421 aggccgtttg tccaccgttg atgccagcgt agcaaatctt gaaaaaggtg aaatcgatgt
     6481 ggcagttgtt tgggacttca atggactaaa ttatcgtgac caaattgact ccgagcgttt
     6541 tgaggtaact gtgccaacag acggctccat cattagcggg tatacaacct taatcaataa
     6601 aaatgcccct catcccaatg cggcaaagct agcacgggaa tatattttat ctgatgaagg
     6661 gcaaatcaat ttggcaaagg gctatgcgcg acctattcgc aaggtagagc tgcctaaaga
     6721 agtagctgaa aaattactgc cggaggatgc ttatgaagcg gcggtgccag ttgaagacaa
     6781 tgaagcttgg aacaagacct ctgtagagct tccagagctt tggcaggcgg aggtactgac
     6841 ctatgcaaag tagacttgac aaactgattg tagttatact agacggctgt cgctacgaca
     6901 cagccgtcgc ccagcttggg ttcatgaatc atttagttga gcaccagcaa gcaagcttat
     6961 tgaaggtaaa aagtgagctg cccagcaatt cacgtccgtt atacgaagta ctgatgaccg
     7021 gcgttccagc ctatgaaaat ggcatctata gtaattacca tgctcagcga tccaaagagt
     7081 ggtcgctgtt tgaattagtt aaggaagctg gcggaaaaac aggagccgcc gcttaccact
     7141 ggtacagcga gttgtacaat caagccccct ataatcgttt ggttgatcgc atccagcatc
     7201 aggaggagcg actgattcag catggcatct tttatagtga ggacacttac cctgattcgc
     7261 atttatttgc ggatgcccat tatttagtga atcagtatca gccagacttt ttggtcgtac
     7321 attcgatgaa tattgatgat gtcggtcaca aatttaccgc cgattctcat gaatatgcag
     7381 cagcggttaa tcaggcggac agattattgg ctatttacct tcctgaatgg ctgcaaaaag
     7441 gttatcaagt cgtggtgact gcggatcacg ggatggatgc gcacggactg catggcggca
     7501 gcttggctgc acatcgtgaa gttccttttt atttgatttc aaataaacgc caaaagctga
     7561 aggaacaaac tgtaccacag ctgctggcgg ctccattgtt ttgctggtta ttaggaattg
     7621 caccgtcaga gaaaatgcag gacattcaag aattgatgag gggatgaaag agatgaaaaa
     7681 acgcttggtt ctggttcctg cctcctttat cttctgtgca tttttactgc tgccgctgct
     7741 ttttatggtt ctgtgcagct tgcacagtga tttaactaac cagtggacat tggccaatta
     7801 cagtgatatt ttcagcaaca aatattacac acaggccttt gttaacagcg tactgattgc
     7861 tgtcagctcc agtgtgattg ggttaatcgg gacgtttatt ttatgtttat gcttaatgaa
     7921 tctatcgact aaattgcagg agaaaatcac ctttgtttcc aatttggcag ctaattttgc
     7981 tgggattccg ctggcattct cttttattat tttgttgggg aatgtaggtg tgctgaaaat
     8041 gattttgcca tttctagcgg acttcaattt gtattcttgg tggggattga ccatcacgta
     8101 tacctatttc caaattccgt taggggtctt gtttttgtat cccagcatga aggaagtgaa
     8161 aaaggaatgg ctggagtccg cacaattgct gggcgcttca tcagcgtatg cttggttcaa
     8221 ggtggctttg ccttttttgc gaccatcgat tgccagcacc tttgtcatct tatttgctaa
     8281 tggcatgggg acctatgaaa cagcgtatgc cctgaccagc agtaatgtaa atcttttaac
     8341 gattcgaatt gcggctttag tgtcgggaga tgtgttcgcc aagcctaatt taggcagtgc
     8401 gttggcagtc gctttaggtg tcctgctggt aacggcaatg ttgattattc aaaagaaagg
     8461 caaggtggtg cagacgtgaa aaagttaata aaaccgattt tgttctgctt tgtcctgtat
     8521 tacttgctgc cgattattgg aacgattttg tatgccagct ccactaaatg gtcaaaatcg
     8581 cttttaccta gtgattttac cttgcaatgg ttccaacagc tgctgacgga cagggaattt
     8641 attgctgcgg tgggtcgctc acttttatta gcaggtgtgg tcttggtgac gattctgttg
     8701 ttgatgattc ccaccattat ttggattcat ttgtatttcc ctcgtttgaa tcgttggctg
     8761 gaaaagctgc tcctgctgcc gtatgcttta ccaggagtga ttttggtaac ggctttgctt
     8821 cgaacgtatg ctgaaactgg tatcccaatg tttgtagtgt tggtaggtgc attgtttatt
     8881 acggcgttgc cgattgtcta tcttagtctt aacaaccaga tgcgcttaat taatctgaag
     8941 gaattagtgg atgcagcaga aaccttgggc gccccgatgt ctacgattat tatccaggtg
     9001 ctgtttccaa acatccgtat cggtgtgacc ctagtttctc tgatgatttt ttcttcggtt
     9061 tttggggagt acatgctgac gaacctgttg attggcggtc gatttcagac ggtacgcatc
     9121 tatatgctgc ggcggatgaa cgaaaacggc catttggcta gcgctgtgat ggttttgtat
     9181 ttgctgttta tggtgggaat tgctttaact acctttatgc tgacttatcg acaaaagcaa
     9241 cagctgtccc ttaagcagac gagggaagag aagcagtcga aaaagaagcc cttaaaggag
     9301 ttgtgacgga aaatgttttt agaaatacat gatctcaatc tgttttttga tgaaaaacag
     9361 attttagcta ttgatgaaat tcagattgaa aagggtgagc tggttacttt actaggtccc
     9421 agcgggtgcg gaaagtctac gttgctgcgc tgtatcacgg gattggaaca accaaagagc
     9481 ggtgagattg tgctggacaa tgagaatatt gccgataagc ctacgaagga tcgcaatatc
     9541 ggctttgttt ttcaacagta tgcattgttt ccaactatga ctgtttttga aaatgtagcc
     9601 tttggtttga aggtaaagaa gctgagtcct gaggtgattc aggaaaaggt ttttgagatg
     9661 ctatcattgg tggatatgac ggagcaggca gataaaaatg tccagtttct ttctggcggt
     9721 caacgacaac gggtggcact ggctcgctca ttggtcactg aaccgaaagt cctgctgctg
     9781 gatgagccat taagtgcttt ggatgcccga attagaaaac agctgcagcg ggatttgcgc
     9841 gctatccagc aatcgctagg gatgacgatg atttttgtga ctcatgacca agaagaggcg
     9901 atgcgaatca gtgatcggat ttttgtgatg gaagctgggc gggtggctca ggtttccacc
     9961 tcgaaggaat tgtatcagaa tcctcagagc cgatttgttg cggaatttat cggcaactat
    10021 aatcgtttcg aatggtatga gctgaatcag tatacccacg agttttccgc taagggcaat
    10081 cgctgcattt attatttaag accggagctg attcaatttg aaccggttcc tcagggggtt
    10141 aagattccgg ttctatggaa ggaatcattt attttaggca acattcaacg gtatgttttt
    10201 caaacgctgg agggcaaaga aattttagtg gatcggttga acgatcggga agagaaaatt
    10261 agcgatgcct tatacattca gctgaaagat attctagcaa tccctgtgga aattgatact
    10321 cagcattttg cacaacaaag ctagtgtata gtcggcaaac atttctggct ctttgtcaaa
    10381 taggactgat agagtgatat aagaacatct aaggtagaat tgattttctg ctttagatgt
    10441 ttttttatgt ttaataagtg atcagatgat tgattaagaa aattctggtt ctcatcctaa
    10501 cccgtaagct attcgtttca atgctttgat gtgggtatgc agattttcta ttttgccatt
    10561 tgagtaaggc aactctaagg ctaaacgaat gccggattca tagtttataa acgctttttt
    10621 tatatgatgc agttctcttg gaaccgactt gggcattgtt tttacaagct gaaagaagat
    10681 gtccgcttct ttgtcataaa aagcttgaag aatttcttga tagtaagcat aagctattcg
    10741 tagttcatca gaagtagaaa gtagacgttc tacgacttca ctttcggtta aataggccca
    10801 accaaaatta cgccattttg tccatttatc ataaacaagc gtgtcctgtc gtttgatcag
    10861 aagtcgagat aaagccttca cttgtcgata gtgtttggct tgtattggat cgtttcggtc
    10921 gaagcttttc atgactcata gtcaaatatc cttaaaactg cgaatgaggt gcttgacgat
    10981 gtgaaagcga tccacaacga gtttggcatg aggaaagcac tcttttatca gcaaagcata
    11041 agaggcgttc atatctgtta cgagtatttt tacgtttaaa cgtgcacgtc gttcaaattg
    11101 cataaaataa gaaatgagat ctttcttctt acgtgaagga agaatatcca gtaatttccc
    11161 agtatcgcca tccatacaag aaaaagaata gggaccgact tgatttttca atgaacgaaa
    11221 ttcatctacc aatgaacagc gaggcaacca atttattttc acgtttgtca tggggagcag
    11281 agaagcgagt gttcgcgaaa cggttgacca agaaacgccg caatgtttag aaatcgtcgt
    11341 cattgcttgt ttttcagtca attcctccaa tattttataa tgtacttgtt tggcgataaa
    11401 acagtttttt tcaacaatgt aggtattcgc cgtaaaatag gtgtgacagc cacgacagag
    11461 gtaccgttgc tttgccaaag caagataagt ccgttgattc gcacacttat tgagaaggac
    11521 cagcgatttt ttctttccgt tcttaacgat cagcccatgt tgattcacct gtttacactg
    11581 aggacagggc tgaatcgttt taatgagcgt ggcagaaaca acgatactgt tgattgaatg
    11641 tatttttttc ttctcgacat gagtgatttg aatgtttcca tctttgattc ctagtaatag
    11701 tttgataaga tgaagatcag gcatacagaa cgctcctttg attgtgttgt ggtgattcaa
    11761 ttctaacgga gcttctgtat gttttgtttt tttatgctca aaaggactga tagaatgatt
    11821 tactatcagt cctagaaatt atagcgccac atttctttca cctttgactt ttcagacggc
    11881 agtttttatg ctgatactgt agagagagca ggacacagct tttgcttgtt ttgggtaagg
    11941 gggaaagaag atgaccaaag cacttatttt tgattttgat gggacgatga ttgacagtga
    12001 atggatgttt tacgagagta ttcatcacta tttactgaag cctatttact ggagcagttc
    12061 ggctataaaa ttccttttgc agactatcaa caaggtgttg ggacggcagc agaactgtcc
    12121 ttttatgaag ggataagtca agcggttggg agaccagtgg atgctgaaca ttttgagcag
    12181 cggatcagca gcactggaaa atcggagtag tctcaattct gaactgcggg agctagacta
    12241 tttttttgaa gcacatccga tgattaagga aaaaattgat tcctttgtga cgatcgatga
    12301 tcttcaggtc ggcaaaccag atccagaagg ttatttgttg tgcttgaaac ggctgagggt
    12361 tgaacaggct gttgtagttg aggatagtcc agtcggtgcc caagcagcac cgcatatgac
    12421 agttgatgta caagcgttgg atgcagattt tttcgccttt agcgggcata aaatgtgcgg
    12481 gccgacagga atcggtgtat tatatggcaa acgtcaattg ttagaacaaa tggagccagt
    12541 cgaattcggc ggtgaaatga tagattttgt caatctttac gatagttcat ggaaagagct
    12601 gccatggaaa tttgaagctg gtactccgaa cattgcagga gcaatcgatt acttgaatga
    12661 gattggtatg gagaatattc atcgttatga gcaagaactg gttgcctacg ttttacctaa
    12721 attacaagcg atcggcggta tcaccattta cggaccgcaa gatcctgaac atcatactgg
    12781 cgtggttgct tttaatttgg atggcatcca tccccgtgat gtggcgactg ctctggatat
    12841 ggaaggtgtc gctgtacgtt caggacacca ttgtgcacaa cggttcatcg acgatttctg
    12901 tttgactaat tggtcgttta aacagtcgat gataatgccg tttgagcggc tccaatgtcg
    12961 tggaatcttt gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt
    13021 cagattacgt gattgatttt cgtacagttc tatgagaatc gcctgcttca gttcctttgt
    13081 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtctgcc cacaatcata
    13141 gcatagaaaa cgggtacgtt ttaattctaa aatagtcaac ttttctttga ctttcaacat
    13201 ctgagtacgt gtcgtatagg aaccgttgcg gataatacga atcgatccac agtgttcaca
    13261 atgggaaggt gagtcatcca atctcccttt aataaactgc gcttgaacgc ctttatattc
    13321 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
    13381 tctagtatga ttatccatag agcgactcct ttggtagggt gatgttttgg tcgactaaat
    13441 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
    13501 ccttcgattt tccaacaaca caaaatatta tagaagcctt ttttctaaag gatttcaagg
    13561 tgtctgtttg catttattaa aaaaaagaaa aagtattggc aacagaaggc gtcaccaaca
    13621 ctagatatta tagagccata atatgctgta taatatctag tgtataggct taagtggagg
    13681 tatatatgaa aatattaact gttgaagatg ataatatgat aagagaggga ataagtgaat
    13741 atctttcaga atttggatat actgttattc aagctaaaga tggaagagaa gccttgtcaa
    13801 aatttaatag cgatataaat ttggttatct tagacattca gatacctttt ataaatggtt
    13861 tagaagtgtt gaaagaaatt agaaagaaaa gcaatttacc aattctaatc ttgactgcat
    13921 ttagtgatga agaatataaa attgatgcat ttactaattt agtagacgga tatgtagaaa
    13981 agccattttc attgccggtc ttaaaagcta gaatagattc tttaattaag aagaattatg
    14041 gacatttaga gaagtttgaa tataaggatc tatc
//
LOCUS       DAEGIQ010000070        13643 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000070 DAEGIQ010000000
VERSION     DAEGIQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.071"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            19..261
                     /gene="yaaA"
                     /locus_tag="KMT45_001875"
     CDS             19..261
                     /gene="yaaA"
                     /locus_tag="KMT45_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBE7005334.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            248..1372
                     /gene="recF"
                     /locus_tag="KMT45_001876"
     CDS             248..1372
                     /gene="recF"
                     /locus_tag="KMT45_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBE7005335.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1369..3315
                     /gene="gyrB"
                     /locus_tag="KMT45_001877"
     CDS             1369..3315
                     /gene="gyrB"
                     /locus_tag="KMT45_001877"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBE7005336.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3335..5806
                     /gene="gyrA"
                     /locus_tag="KMT45_001878"
     CDS             3335..5806
                     /gene="gyrA"
                     /locus_tag="KMT45_001878"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBE7005337.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6042..6341
                     /locus_tag="KMT45_001879"
     CDS             6042..6341
                     /locus_tag="KMT45_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBE7005338.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6391..6903
                     /gene="ssb"
                     /locus_tag="KMT45_001880"
     CDS             6391..6903
                     /gene="ssb"
                     /locus_tag="KMT45_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE7005339.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6929..7165
                     /locus_tag="KMT45_001881"
     CDS             6929..7165
                     /locus_tag="KMT45_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBE7005340.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7308..9281
                     /locus_tag="KMT45_001882"
     CDS             7308..9281
                     /locus_tag="KMT45_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBE7005341.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9287..9739
                     /gene="rplI"
                     /locus_tag="KMT45_001883"
     CDS             9287..9739
                     /gene="rplI"
                     /locus_tag="KMT45_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBE7005342.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10013..11380
                     /gene="dnaB"
                     /locus_tag="KMT45_001884"
     CDS             10013..11380
                     /gene="dnaB"
                     /locus_tag="KMT45_001884"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBE7005343.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12160..13452
                     /locus_tag="KMT45_001885"
     CDS             12160..13452
                     /locus_tag="KMT45_001885"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBE7005344.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4541 a         2475 c         3007 g         3620 t
ORIGIN      
        1 gaaaatgagg gatgggcatt gaaacaacaa gtcattctaa atacggaatt catgacgttg
       61 ggccaaatgt tgaaagaagt cacagttatc ggtagtggcg gacaagcaaa atggtatttg
      121 gcagaaaaca ctgtgttagt cgatggcgaa ccagaaaatc gtcgtggacg caaactttat
      181 cccggcatga tggttgaagt tccagaagta ggaacttttt ttatggtaaa aaaaggaaac
      241 gacgataatg aggctgaata agctgtattt aaaaaattat cgaaattacg aagagctgaa
      301 catcgaattt tccaagaacc tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa
      361 tcttttggaa agtatctacg tattagctat gacgcgaagt catcggacaa gtagtgagca
      421 ggaattgatt ggctggactg acgatcaggc gatgattcaa ggagagatca caaaaggttc
      481 atccactctt ccattagaaa tcttattatc taaaaaaggc cgaaaaacca agatcaacca
      541 tatcgaacag aaaaagttaa gtacttacgt gggacaaatg aatgtcattt tatttgcacc
      601 agaagatctt tctttagtaa aaggcagccc gcagatcagg cggaagtttt tagatatgga
      661 attaggccag atcaatccaa tctatcttta tgatcttgtc caataccaag caattttgaa
      721 acaaagaaac cagtacttga agcaattgaa tgaaaaaaaa caaactgatt tgctttattt
      781 ggatattttg tccgaacaac tagcagcgtt tggcagcaaa gtattgaaag caagagcaca
      841 gttcgtcaag cgattggagt tttgggctaa ttctttgcat cagcaaatca cccatcaaaa
      901 agagcagctg gaaatcgagt atctcaccgc agtcgattct ttagaaacac atacgcaaga
      961 gcaaatccaa gaacaatttc tcgcactttt gaaccaaaat aaaaagaaag acctttttcg
     1021 gggaacaacg acagttggtc ctcatcgtga cgatctttcc ttttttatta atcagaaaaa
     1081 tgtgcagact tatggatcgc aaggacagca aagaacaact gcactaagtg tcaaattagc
     1141 agaaatcgat ttgattaaag aagagacagg ggaatatcca atccttttgt tagatgatgt
     1201 gatgagcgag ctggatgata atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt
     1261 tcaaacgttc ttaacaacaa cgacacttga ccatgtaaaa aataaaatga ctgttgaacc
     1321 tgagattttt tatgttcaac aagggaaact aaaaggagaa gaaactgaat gacagaagaa
     1381 agaagtttag tagaacgcgc taaagagtat gatgccagtc agattcaagt acttgaagga
     1441 cttgaagccg ttcgtaaacg acctggcatg tatatcggtt cgactagttc tgagggtctg
     1501 catcaccttg tttgggaaat tgtcgataat tcgatcgatg aagtattagc aggttttgca
     1561 acgaaaatcc atgtgattat tgaaaaagac aacagtatca ccgttattga cgacggtcgg
     1621 gggatacctg tagatatcca ggcaaaaact ggacgtcctg cagttgaaac ggtctttact
     1681 gttttacatg ctggaggaaa attcggcggt ggtgggtaca aagtttctgg tggacttcac
     1741 ggagtaggat cttctgttgt aaacgcgctt tctacacagc ttgatgtcaa agtatacaaa
     1801 gaaggaaatg tgtactatca agaatatcgt cgtggagctg tagtggacga cttaaagatt
     1861 atcgaacaaa cggatcgcca tggaacaacg gttcacttta cgccagaccc tgaaattttt
     1921 acagagacaa ctgagtttga tttttcaaaa ttggctaccc ggatccgtga acttgctttc
     1981 ttgaaccgcg ggatgcgtat atcgattgaa gacaaacgag aagaagaacc agtcatcaat
     2041 gaataccatt atgacggcgg gatcaaaagt tatgtcgaat acttgaatgc aaacaaaacc
     2101 gtgatttttc cagaacctgt atatttggaa ggtgaacagc aagatatcgc agttgaagtc
     2161 tctatgcaat atacagacgg ctaccattcc aatatcatga gttttgccaa caatatccat
     2221 acgtatgaag gtggaacaca tgaatcagga ttcaagactg ctttaactcg tgtgataaat
     2281 gattatgccc gaaaacaaaa gctgatgaaa gaaaatgacg acaacttgac cggtgaagac
     2341 gtgcgagaag gattgacagc tgttatttct atcaagcatc ctgaccctca attcgaagga
     2401 caaacaaaaa caaaattggg caattccgaa gtacggacag tcactgatcg gttatttagc
     2461 gaacatttca tgaaattctt gctggaaaac ccaagtgtgg gccggcaaat tgttgaaaaa
     2521 ggattactag cttccagagc tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt
     2581 aaaggtgctt tagagattag taatctgcca ggaaaattag cggactgctc tagcaacgat
     2641 cctgaaaaat gcgaattgtt catcgtcgaa ggagattcgg ccggcggatc agctaagcaa
     2701 ggtcgtaatc gagagtttca agccatcttg cctattcgtg ggaaaatctt gaatgttgaa
     2761 aaagcaagca tggacaagat cttggcaaat gaagagatcc ggtctttatt tactgctatg
     2821 ggtacagggt ttggagaaga ttttgatgta tcgaaagctc gctaccacaa attagttatc
     2881 atgactgatg ccgatgtcga cggcgcgcat atccgtacat tgctgctgac tcttttttat
     2941 cgttacatgc gtcctattgt tgaagctgga tacgtctata ttgctcagcc acctttatac
     3001 ggggtaaaac aagggaagaa tattacgtat gtacaaccag gaaaaaatgc agaagaagag
     3061 ttgaaacaag tcgttgcttc acttcctgcc tcaccaaaac caagcgttca gcgttataaa
     3121 ggacttggag aaatggatga tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg
     3181 atgatggccc gcgtcagtgt ggacgatgct attgcagcag atcagatatt tgaaatgttg
     3241 atgggggatc gagtagaacc ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag
     3301 aacttagata tttaatcatg aaggaggaaa aagaatgagt gaagaaatca gagaaaacat
     3361 ccatgatgtc aatctaacca gcgaaatgaa agactccttc atcgattatg cgatgagtgt
     3421 tattgtcgca cgtgcacttc ctgatgtcag agatggtctg aaacccgttc atcgccgtat
     3481 tttatacggc atgaacgaat taggtgtaac accggataaa gcccataaaa aatctgccag
     3541 aatcgtcgga gatgtcatgg gtaaatacca tcctcatgga gacattgcga tctatgagtc
     3601 aatggtacgg atggcacagc cattcagcta ccgttatatg ctagtggatg gtcatggaaa
     3661 ctttggttct gttgacggtg acggtgccgc cgctatgcgt tataccgaag cacggatgag
     3721 caaaatcgct acagaaatgc tgcgtgatat caataagaac actgttgatt tccaaagcaa
     3781 ctatgatgat acagaaaaag aaccagttgt actacctgct cgcttcccga atcttttagt
     3841 gaacggaaca acggggatcg ccgtaggtat ggccacgaat atcccacctc ataacttgtc
     3901 tgaggtagta gcggctatcg atttgctgat ggataatcct gatgtgacga ccaatgaatt
     3961 gatggaagta ttacctggac cagattttcc aacaggtggt ttggttatgg ggaaatcagg
     4021 aatccgaaga gcgtatgaaa caggtaaggg ttctattacc gtacgtgcaa aagttgagat
     4081 tactgagatg ccaaatggta aagaacggat catcgtgaca gaattgcctt atatggtcaa
     4141 taaagcaaaa ttgatcgaac ggatttctga actgcacaga gacaagcgta tcgaagggat
     4201 cacagatttg cgggacgaat cttcccgtga aggtatgcgg atcgtcattg acatccgtcg
     4261 tgacgccagc gcatccgtta tcttgaataa cttatataaa ttgacatcct tgcaaaattc
     4321 atttggattc aatatgttgg cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca
     4381 aatccttgaa aactatatcg aacatcaacg cgaagtcatc acacgacgga cggtctttga
     4441 aaaagaaaaa gcagaagcaa gagcacacat cttagaaggt ttgcgtattg ctttggatca
     4501 tatcgatgaa atcattgcga ttatccgcgg gtctaaatca gatgacgagg caaaagcaac
     4561 aatgatcgaa cggttcgatt tatccgatcg ccagtctcaa gctattttag atatgcgttt
     4621 acgccgattg accggtttgg aacgggaaaa aatcgaaaat gaataccaag aactcttaaa
     4681 actgattgaa gatttaactg acattctggc aagaccagaa cgtgttacag aaatcatcaa
     4741 gaatgaatta gcagacttga atcaacgatt tggtgataaa cgacgcacag aattactagt
     4801 cggtgaggtc ttaagtctgg aagatgaaga tttaatcgaa gaagaagaaa tcgttattac
     4861 attgacaaac aatggttaca tcaaacgttt ggcaaataat gaattcagag ctcaacgaag
     4921 gggtggacgt ggtgtccaag gaatgggtat ccacaatgac gacttcgtca aaaatctggt
     4981 ttcttgttct acccatgata cattgttatt ctttacgaat aacggaaaag tgtatcgggc
     5041 aaaaggatac gaaattccag aatacgggcg tacagccaaa ggaatcccaa tcatcaattt
     5101 actaggtatc gattcgagtg aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga
     5161 agaaggccac tatctcttct ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac
     5221 tgcttttgct aacatccgaa gtaatggtct gatcgcaatt ggtttgaaag aagacgatga
     5281 gctcgtcaat gtgctactga cagatggtca atctaatatc attattggaa cacataacgg
     5341 ctactctgtc actttcaaag aagaagcagt ccgcgatatg ggacgtacag cttccggggt
     5401 cagagggata cgcctgcgtg aggaagatta tgtcgttggg gcagccttaa tgaaggaaaa
     5461 tcaagaagta ttagtgatca cagaaaaagg ttacggaaaa cggacaaaag tatcggaata
     5521 tccagtgaaa ggccgcggag gtaaagggat caaaacggcg aacatcactg aaaaaaatgg
     5581 tccactagct ggattgacca ctgtttctgg agatgaagac atcatgctga ttacagataa
     5641 aggtgtcatt atccgtttca acgtatcgac tgtttctcaa actggacgtt caacattagg
     5701 cgtacgtttg atgaagatgg aagctgatac gaaagttgtc acgatggcag cagtcgaacc
     5761 agaagctgca gaacaaacag ttgaagaaca aggatcagaa gaataaccat caagactaag
     5821 tgtgtaagct agcaagaata accgattaaa aaatctggtt attcctgctg gcttttttga
     5881 ttagttgaat ttctcttaaa atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa
     5941 tagagtgtat aattagagct tgtgagtagt ctaattaatt agataactct ccttgctctt
     6001 aggaacctaa gagccgaaag tccacaggga ggtgaatatc aatggaaaac acgaaatatg
     6061 aaatcatgta cattattcgt ccaaacattg atgaagaagc aaaaactgct ttaatcgaac
     6121 gtttcgatgc aatcttgaaa gataacggag cagaagttat cgaatcaaaa gattgggaaa
     6181 aacgccgttt cgcttatgaa atgaacggtt accgtgaagg tatctatcac atcgttaaag
     6241 tatcttctcc atcatcagca aatgctgtca atgaatttga ccgtcttgct aaaatcaacg
     6301 acgatatcat tcgtcacatg attgttaaag aagaagctta attgttgttt catgtgaaac
     6361 aatagaaaac gatgagagga gatgactatt ttgattaata atgttgtatt agttggacgt
     6421 ctgacgaaag atccagattt gcgttacaca gcaagtggta cggcagtcgc aacatttact
     6481 ttggctgtga atcgtaattt tacaaaccaa agcggcaatc gcgaggctga ttttatcaac
     6541 tgtgtgattt ggcgcaaatc agcagaaact ttagcaaact atgctcgtaa aggaacactt
     6601 ttaggtgtta ctggacgtat ccaaactcgt tcttatgata atcagcaagg acaacgtgta
     6661 tacgtgacag aagtagtagc tgacaacttc caattgctgg aaagtcgttc tgcatctgaa
     6721 aatcgccaac aaagcggcgg attccaaagt tctggtcaat cagctggcgg atttggcgga
     6781 aacaataact ctaatcaaac atctcaatca tccaacggta tgcctgattt cgaccgtgat
     6841 acttctgacc cattcggctc aagttcaacg atcgacattt cagacgatga tttaccattc
     6901 taaacgaatt tgataggagg gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg
     6961 tcgtaaagta gactacattg ctgctaacca tattgaatat atcgattata aagatatcga
     7021 attactaaaa agatttattt cagaacgcgg taaaatcttg ccacgtcgtg taactggtac
     7081 aggcgctaaa aaccaacgta aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt
     7141 gctacctttc gttagcgaag actaataaat cctaggaatt gaatgactga tccttttgat
     7201 cagctattca attccttttt ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg
     7261 acgaaagtta tggtaaaatc aaattagtca tatatgggag gaaaattatg gaaaataaaa
     7321 acaatcattt atccccgaaa cctctcttcc ttatcctgtt tatattaatc gaagtattgg
     7381 tagtcttaat tttaccattt cgttttgtta gcgcttcggt cattctattg atgaatttat
     7441 ggggtgttcg ctattggttg aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa
     7501 tccagcaggc ttcgactgta gctgaaacca atctagctta tgtctcagaa gtcatgcctg
     7561 taggagtgat tgcttatcag ccatcaagcc aaaaaatcga atggctcaat ccattagcat
     7621 tgaaaatcaa agatcaaagt ggattaacag aggaagaatt gctagatact ttttttactg
     7681 caaaagaaaa aaagaaaaac caagtctatc tagcagataa aacatttcag tttgatatgg
     7741 atacagtaaa gggtgttgtt tattttaatg atattaccca aaaccagttg ttaaaactcc
     7801 atcagacgaa cagtcagccg gtgattggta ttttatctat tgataactat gcagatgcca
     7861 ttgataaatt agatgacaaa gagatttctt atttgaactc tttcgtcact acactagttt
     7921 ctgactggat gaatgaatac catgtctttt tcaaaagaat caacgcagaa cggtttttct
     7981 tcgtagcaca gatgatcgat ctgaaaaaga tgatggataa aaaatttgat ttgctggatc
     8041 gctttcgagc agaagctgaa aaacagagct cgcctatcac ccttagtatg gggattgctt
     8101 atggctcaag aaccctcgat gaaatcggtg gagaagctca gaataattta gatgttgctt
     8161 tagtccgtgg aggcgaccaa gttgtcgtga aagaggtaaa agaaggagca aaacctgttt
     8221 attatggggg gaaatctgct aagaatgtca aacgcacgcg tgttcgttct cgagcgatga
     8281 gtaccgcttt gcgtcggatt tttgatgaaa caggcgatgt gtttatcatg gggcacaagt
     8341 ttccggatat ggatgcttta ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc
     8401 agaaaaaagc ttacatcgtt atcaatgaga acgagatcat tccagatgta gaacggtgtc
     8461 ttgaagaaat ccataagcat cctgaattag aaatgcaact aatctctcca gaacgtgcca
     8521 tagagctgaa aaaagaagag gacctcctcg tgatggtgga ttaccataaa ccttctctat
     8581 cgatctctca gggcctatat gagcaatttg ataagattgt gatcattgat caccatcgtc
     8641 gtggggaaga gtttccaagt aaacctctgc tgacttatat tgaatcttca gcttcttcag
     8701 cttcagagct agtttctgag ctgatccaat accaaagcag caaaacaaat cgcttagatc
     8761 gttttgaggc cacattattg cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc
     8821 gtacaactgc acgaactttt gacgttgcca gctatttaag gtcttgtgga gcagacgcat
     8881 ccttagtaca atatttattg agctcggatt tagcgacata cttggagatc agcaatttga
     8941 ttgctggaag cgactatgtg acttctgata ttgttgtggc ggcagggaca gacgaaaaag
     9001 agtataatag tgttatagca gcaaaaacag cagatacatt gctttcgatg atcaatatca
     9061 atgcagcttt cgtcattaca aagcggacag acggcttgat tgggatcagt gccagaagta
     9121 ccggcacgat caacgtccaa atcatcatgg aatccttagg tggcggagga cacttcacaa
     9181 acgcagctac tcaactggaa aatacgacag ttgaggaaac aaaacagaag ctatttgctg
     9241 caatcaaaga aaatatgaat caaatatatg ataaggagta atccaaatga aagtaatttt
     9301 tttacaagat gtaaaaggaa aaggtaaaaa aggggacatc aaagaagttc cagcaggtta
     9361 tgcacaaaat tatctaatca aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag
     9421 tcagttagca ggacaaaaga aagcacaaga aaaacacgaa gctgaaatct tggaagaagc
     9481 taaaaagtta aaagaattct tggaaaaaga agatactgta gtcgaattga aggcgaaagc
     9541 aggggaagac agccgtttgt ttggttcgat cccttcaaaa caaattgctg aagggttaaa
     9601 caaacaatac aaaatcaaat tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc
     9661 actaggctac acaaacgtac ctgttaagct tcatcatgag gtgacggcta cgatcaaagt
     9721 tcatgtcgta caagaataac aaatcttgat agcgatgttc agctggtaag tataaccaga
     9781 aggaaaaaag acaaaccgaa atttatctga gaaaacagct gcagcaatga aagcaaaagc
     9841 tgcaaatcga tttcagattg atttcggttt cttttttggg aacagcaaaa tagcataaag
     9901 aaaacgagta taataaaaga gaactatttt tttattacac tttcggtacg aatgatgact
     9961 tttctctcat tttcttgtaa aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga
    10021 agtgtggcag gatcgaatcc caccgcaaaa tattgaagct gaacaagcgg ttttaggctc
    10081 tgtttttctt gacgcagaaa cgatcattga tgccatggag tacatagagc caaaagactt
    10141 ttatcgtcgt ggccatcaaa ttatttttga gacaatgatc gagttaaatg atcgtaatga
    10201 agcgattgac gtggttactg tcaaagaccg gctggaacaa gccaatttgt tagaagatgc
    10261 cggtggttta agttatctct cagacttggc attagcagtt ccgacagcag caaatatcgt
    10321 ctactatgcg aaaatcgttg aagaaaaatc attgctgcgt acattgatcc agacagcaac
    10381 aggaatcgtt acgaaaggat tcgagcaagg agaagaagtt caaacaattt tagatgatgc
    10441 cgaacgcagt atcttggagg tttcggaaaa aagaaatcga agcggctttt tatctattgc
    10501 tgaagtactg aactccacga ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat
    10561 cacaggtcta ccaactggct atcaaggtct ggataaaatg acagcaggtt tccagcccga
    10621 agaactaatc attcttgctg cacgtccagc ggtaggtaaa acagcgtttg cattgaatat
    10681 tgctcaaaat gtaggaacaa aaacagatcg ttctgttgcg atatttagtt tggaaatggg
    10741 ggcagaatct cttgtaaatc ggatgctttg tgcagaaggt tcgattgagg ccagccattt
    10801 acgtacaggt cagctttcgg aagaagaatg gtcgaatctg atcgttgcga tggggagtct
    10861 ttctcgagca aatattttta tcgatgatac accagggatc aaaatctctg aaatccgtgc
    10921 gaaatgtcgg aaactagcgc aagaaaaagg caatctaggc ttgatattga tcgattattt
    10981 gcagttgatt gaaggtaacg gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg
    11041 ccaactgaaa aaactggcga aagagctgaa agtacctgtc attgctcttt cacagttatc
    11101 tcgtggagta gaacagcgac aagacaaacg accagtgcta agtgatattc gtgaatccgg
    11161 atcaattgaa caagatgcgg acatcgtagc cttcttgtat cgagatgatt attatgaaag
    11221 agaaggcgga gatgacgatg atggaaacga gccaaaaaat gacaacgtca tcgaagtcat
    11281 catcgagaag aaccgaagcg gtgcacgagg aacagttgaa ctactgttta tcaaagaata
    11341 caataagttt tcgtcgcttt cgccaagaga agaattttaa agaagaggtt gtgagccttg
    11401 agcaatcccg agagctctcg taagaagatg caaggagtaa ccgcaggaac gactagagta
    11461 cttaatttaa acagatctcc aaagggattg tgacaaaagt cttgtcacag tccctttttc
    11521 cgaataaacg gtgttcaatc agctgaccct tggtattagc tcagcaaacg tcaaaatatg
    11581 aggaactatt ttgacgtttg tttctctaat actcaggtca aaacgctgat cttacgacct
    11641 cttttcctta aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatcatcaaa
    11701 catgagaagc agttttttga aattgctcct tgtgactcag agttaaacgg caggctcaca
    11761 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taatacgccc aatcaaacga
    11821 aaaatatgag aagctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt
    11881 cacaacctct gattcacggt gttccaaaag tagcttcttc gaaaaataag tcgaaatttc
    11941 acaaaaattt gagaagcaat tttcgtacat tccttcttat ttcttgaagc tgaccacttc
    12001 tgttacaacc tcttttttaa taaccattct aaaaagataa agttcgtttt ttaagcgttt
    12061 ttataaaata aaaataaaaa tcattcgatt ttatttgaaa tatgtatcta ttattgatac
    12121 aatggttcag gattaaataa tatagaacga ggtgttcaca tgtcatctgt agtagtagta
    12181 ggtacgcaat ggggcgatga agggaaagga aaaatcacag actttctcag tgaaaatgct
    12241 gaagtgattg cacggtacca aggtggcgac aatgcagggc acacaattaa gtttaacggt
    12301 gtgacttata aactgcattt gattccttct gggatttttt ataaagacaa gatcagtgtg
    12361 atcggaaacg gcgttgtagt caatcctaaa tcattggtta aagaattagc ttatttacat
    12421 gaaaaaggtg tagaaacaag taatttgcgt atttctgacc gcgcgcacgt aatcttgcct
    12481 tatcatatca aattggacca attacaagaa gatgctaagg gcgaaaataa gattggtaca
    12541 acgattaaag gaatcggacc tgcctatatg gataaagcag ctcgtgtagg tatccgtatt
    12601 gcagacttat tagataaaga aatttttgaa gaacgtcttc gtttgaatct agatgacaaa
    12661 aaccgtcaat ttgtaaaaat gtttgattca gaaccacttg attttgacga tatttttgaa
    12721 gaatattatg aatacggaca acaaatcaaa caatacgtca cagatacttc cgttatcttg
    12781 aatgatgctt tggatgcagg caaacgcgtg ttgtttgaag gcgcacaagg tgtaatgctg
    12841 gatatcgacc agggaactta tccttttgtt acttcttcta acccagtagc cggtggggta
    12901 acaatcggaa gcggtgtagg gccttcaaaa atcaataaag ttgtcggcgt ctgcaaagcc
    12961 tatacgtcac gtgtcggtga tggaccattt ccaactgaat tatttgatga aacaggagaa
    13021 caaatccgcg aagtaggacg agaatacggt acgacaactg gtcgtccgcg tcgtgtaggc
    13081 tggtttgatt ctgtagttat gcgccattct aaacgagttt caggtattac caacctatct
    13141 ttaaactcga tcgatgtatt aagtggattg gaaacagtaa aaatttgtac ggcttatgaa
    13201 ttagatggcg aactaatcta ccactatcca gcaagcttga aagagttgaa ccgctgcaaa
    13261 ccaatctatg aagaattgcc aggctggtca gaagacatta caggatgcaa aacacttgca
    13321 gaactgccag aaaacgcacg taactatgta cgtcgtatct cagagcttgt tggtgtacgt
    13381 atctcaacat tttctgtcgg acctgataga acacaaacaa acatcttaga aagcgtctgg
    13441 gcgcaaatct aaggataaaa acgacgactg aatcaggacg ttcttaccag agcgagtcga
    13501 agtgtttatc tatacgataa taataacttg aagaggtagt gaaagagacg tttagcctta
    13561 ttgccgatag gctgtctctt gaacactgtt tattcagaat aagggactgt gacaaaaacc
    13621 gtgtcacagt ccctttttcc gaa
//
LOCUS       DAEGIQ010000071        13559 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000071 DAEGIQ010000000
VERSION     DAEGIQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.072"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            226..894
                     /locus_tag="KMT45_001886"
     CDS             226..894
                     /locus_tag="KMT45_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBE7005345.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="KMT45_001887"
     CDS             complement(1094..1324)
                     /locus_tag="KMT45_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005346.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="KMT45_001888"
     CDS             complement(1451..2833)
                     /locus_tag="KMT45_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBE7005347.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="KMT45_001889"
     CDS             complement(2989..4176)
                     /locus_tag="KMT45_001889"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBE7005348.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="KMT45_001890"
     CDS             complement(4183..5214)
                     /locus_tag="KMT45_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBE7005349.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATAAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="KMT45_001891"
     CDS             complement(5230..6684)
                     /locus_tag="KMT45_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7005350.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="KMT45_001892"
     CDS             6859..7764
                     /locus_tag="KMT45_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBE7005351.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="KMT45_001893"
     CDS             7855..8532
                     /locus_tag="KMT45_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBE7005352.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="KMT45_001894"
     CDS             8529..8699
                     /locus_tag="KMT45_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005353.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="KMT45_001895"
     CDS             8813..9709
                     /locus_tag="KMT45_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBE7005354.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="KMT45_001896"
     CDS             complement(9764..11134)
                     /locus_tag="KMT45_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE7005355.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="KMT45_001897"
     CDS             11372..12118
                     /locus_tag="KMT45_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBE7005356.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="KMT45_001898"
     CDS             12115..12954
                     /locus_tag="KMT45_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBE7005357.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..>13559
                     /locus_tag="KMT45_001899"
     CDS             12955..>13559
                     /locus_tag="KMT45_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBE7005358.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENK"
BASE COUNT         4327 a         2599 c         2689 g         3944 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcagctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaa
//
LOCUS       DAEGIQ010000072        13424 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000072 DAEGIQ010000000
VERSION     DAEGIQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.073"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            79..1125
                     /locus_tag="KMT45_001900"
     CDS             79..1125
                     /locus_tag="KMT45_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBE7005359.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="KMT45_001901"
     CDS             1474..2106
                     /locus_tag="KMT45_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBE7005360.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="KMT45_001902"
     CDS             2694..4133
                     /locus_tag="KMT45_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7005361.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="KMT45_001903"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="KMT45_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="KMT45_001904"
     CDS             4895..5110
                     /locus_tag="KMT45_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005362.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="KMT45_001905"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="KMT45_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="KMT45_001906"
     CDS             complement(5770..6681)
                     /locus_tag="KMT45_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBE7005363.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="KMT45_001907"
     CDS             complement(6700..7704)
                     /locus_tag="KMT45_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBE7005364.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="KMT45_001908"
     CDS             complement(7701..9824)
                     /locus_tag="KMT45_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBE7005365.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="KMT45_001909"
     CDS             complement(9829..12276)
                     /locus_tag="KMT45_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE7005366.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="KMT45_001910"
     CDS             complement(12263..12652)
                     /locus_tag="KMT45_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBE7005367.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="KMT45_001911"
     CDS             complement(12712..13215)
                     /locus_tag="KMT45_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBE7005368.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13389)
                     /locus_tag="KMT45_001912"
     CDS             complement(13228..13389)
                     /locus_tag="KMT45_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005369.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4107 a         2645 c         2458 g         4214 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctaacaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac catt
//
LOCUS       DAEGIQ010000073        13304 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000073 DAEGIQ010000000
VERSION     DAEGIQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.074"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            813..1286
                     /locus_tag="KMT45_001913"
     CDS             813..1286
                     /locus_tag="KMT45_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBE7005370.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1295..1522
                     /locus_tag="KMT45_001914"
     CDS             1295..1522
                     /locus_tag="KMT45_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005371.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2158..2529
                     /locus_tag="KMT45_001915"
     CDS             2158..2529
                     /locus_tag="KMT45_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005372.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2785..3027)
                     /locus_tag="KMT45_001916"
     CDS             complement(2785..3027)
                     /locus_tag="KMT45_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBE7005373.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3059..3961)
                     /locus_tag="KMT45_001917"
     CDS             complement(3059..3961)
                     /locus_tag="KMT45_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBE7005374.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3974..4162)
                     /locus_tag="KMT45_001918"
     CDS             complement(3974..4162)
                     /locus_tag="KMT45_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBE7005375.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4176..4739)
                     /gene="amaP"
                     /locus_tag="KMT45_001919"
     CDS             complement(4176..4739)
                     /gene="amaP"
                     /locus_tag="KMT45_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBE7005376.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4777..5670)
                     /locus_tag="KMT45_001920"
     CDS             complement(4777..5670)
                     /locus_tag="KMT45_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE7005377.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5748..6671)
                     /locus_tag="KMT45_001921"
     CDS             complement(5748..6671)
                     /locus_tag="KMT45_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBE7005378.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6720..7070)
                     /locus_tag="KMT45_001922"
     CDS             complement(6720..7070)
                     /locus_tag="KMT45_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBE7005379.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7103..8005)
                     /locus_tag="KMT45_001923"
     CDS             complement(7103..8005)
                     /locus_tag="KMT45_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBE7005380.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7998..8855)
                     /locus_tag="KMT45_001924"
     CDS             complement(7998..8855)
                     /locus_tag="KMT45_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBE7005381.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9189..9998
                     /locus_tag="KMT45_001925"
     CDS             9189..9998
                     /locus_tag="KMT45_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBE7005382.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10038..10535
                     /locus_tag="KMT45_001926"
     CDS             10038..10535
                     /locus_tag="KMT45_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBE7005383.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10920..10993)
                     /locus_tag="KMT45_001927"
     tRNA            complement(10920..10993)
                     /locus_tag="KMT45_001927"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10957..10959),aa:Ile,seq:cat)
     gene            11180..11503
                     /locus_tag="KMT45_001928"
     CDS             11180..11503
                     /locus_tag="KMT45_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005384.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11667..11921
                     /locus_tag="KMT45_001929"
     CDS             11667..11921
                     /locus_tag="KMT45_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005385.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11991..12236
                     /locus_tag="KMT45_001930"
     CDS             11991..12236
                     /locus_tag="KMT45_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005386.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12312..12677
                     /locus_tag="KMT45_001931"
     CDS             12312..12677
                     /locus_tag="KMT45_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005387.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12738..13301)
                     /locus_tag="KMT45_001932"
     CDS             complement(12738..13301)
                     /locus_tag="KMT45_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005388.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         3875 a         2462 c         2234 g         4733 t
ORIGIN      
        1 tgatgaaaag attgtactat tcattgatca taacgattgg ctatttgatt gtatcaaatc
       61 taggaaatat ggtatttggt atcagtaaag aattcagttg gactactaca ttatgggaat
      121 ctctattctt tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa taaacatgtg
      181 ccatctatct gttggcacat gttttaggat ttatggtact ttttattcgg caatccattt
      241 tatttttcta cttcttctta atacctctaa cacttttgat ttataaacta atccaatgca
      301 tctacttgcg cttattaagc tatttcagct agatagtacc accccatgcg tcaatgcttg
      361 attttccttt tgtataacat tgtataagca ataaaggaca aataagtggt gattacacca
      421 aaccattgaa taagaaaaat tctgatacgt aaataaacat gaacttgaaa aaattaatga
      481 atcggttagc tactacgtag ttgtcttttt tctcatacag ttctgttaac ttacacttct
      541 cttcgctaga taataaaaaa tcctccaact gtattgtcca atataatccc aggcatctaa
      601 actagcatta tagaagagac acacatagaa tctaaaataa ctggttttaa atgtttctta
      661 caatcaaaaa aagctgttaa atgcactcca caccttcata ctctatcatt ttttataaac
      721 gatatatttt tattgttttt cgataaaaaa tagttgtttt acaataatta ttctgctata
      781 ttttagctat caaagataaa agagggataa aaatgaaatt taaaacattg ctattaaaat
      841 ttgtcgtttt aggtataagt gccgtattta ttttgttcgg cgtacttttt tatactcaga
      901 tcgctacaag cgaaaaagga ttcaattttg attggcagac cacattattt gtttctgtca
      961 tttactttgc ccttctccta gggatcgtta ttgcttattt cttattgaaa ctattgaaaa
     1021 aaatcgaaga taaccaagct ttttccactc aaagcttaaa catactaaaa aaaatcgaac
     1081 gaaccatttt gtttattggt ttcgcctttt ttggcttact tcctaaattt atgaaagtag
     1141 cggatttaga cgatgctcca ggtttgatgt tggtaggaat cgctgtcgta ttcattcctt
     1201 ttgcagtata cacattaaca agcgtgctgg aaaaactgct tgaacatgcg attttactta
     1261 agacagatca tgatttgaca gtgtgaggtg cgacatggca attatcgtta atttagatgt
     1321 agtattagct caaaaaaaaa tgagtgtgac tgaattgagt gagaaagtgg gcattacgat
     1381 ggcaaacatc tctattctaa aaaatggaaa agcaaaagcc atccgttttt ccactctcga
     1441 aaaaatctgc caagttttgg agtgtcaacc aggtgatttg ttgacctata aaaatgactc
     1501 gaatactata ccagaagaat aaaataaagt agaacaaaca gtaaataaaa aataatctga
     1561 atgatagatg tgtgagactt ttagaagtta tttacgtcta ttattcagat tttttatttc
     1621 gattgaatac cgttttatct ataaataaga tatgtactga ctcatacaaa gggggccttt
     1681 tcttctccat gtactatcac ttttcctgat acaactggtt tcctgttacg ttgtagttgg
     1741 atctgctttt agtataatac tttattcatt ccaccagact gatagcagtt tgagcagtct
     1801 aaataatcat gtagtattac aggccattct gtttcatacc ttgaagtgct taaaacttca
     1861 gctaatggcc cattgtgtta actcttactc cattcttttt tagtaagctc ccatatatac
     1921 tgtaaagcct tctatatatt atgtcatgtt gctttttaac atttactatg tagttttgat
     1981 aaaatgattt gaataacagt gatggacaat tcttttttat ttttaacatt attttgataa
     2041 taatgattat ttatttaaaa atacattaga taacatttgt tacgacacga aaaagcttca
     2101 caaacaaata tcgttatttt aaaatgaggg cgaactcaca caattaggag gaatagtatg
     2161 ccatttattg tttacttttt tctttcatta ctcgtgatct atattccatt accaacattt
     2221 ttcctgctcg tcaatcgtct agcagagcaa catgatacga ttgctgctct tacgaaactt
     2281 tgtttacttc tatttgtttg tctagattac tttacgagct attattggct aaaacagaca
     2341 agagtaaaaa agagatatta tttcttcctt gtctttttca atctctttga gtttctgatc
     2401 cattgctttt tacttcatca gtatcatacg atccatttgt ggatcatctg ggttttcaaa
     2461 gtggtcgtat tattttgtat gttcaccttt cctatctcaa gaaaatttag ggaggcattg
     2521 ttttattaaa gaagcttctc tgtatcaata aagacacatt taaggaaaaa cttttaaaaa
     2581 tctaattggg aatttgcccg cataaataag aggctgtgac aaaagtcttg tcacagcctc
     2641 ttattccgta taaacagtgt tcaaaagctt gcatcttcga taatatgccc aaccaaatga
     2701 aaaatatgag aagctatttt tgtttggctg ttcttattac ttgatgaaga acagcttttt
     2761 cacgacctct ttttaaaagt ctttttacag ttttcgagca aagaatgata cgattagtac
     2821 taaaatgatg gcaccaatca atgatggcac gattgccatt cctgcaatac ttggtcccca
     2881 agaacctaat aatgcttgac caagtgctga tccaactaaa cctgctacga catttgcaat
     2941 aatccccatc gaagaacctt tattcgtgat tgctcctgca attgctccga taattcctcc
     3001 gacgataagt gaccaaataa atcccatgtt tcgttcctcc taatgtttta cttccttatt
     3061 actgaacccg tggttctgta ttttctttta ctttttgtgc gccgcttcca gctgctgatt
     3121 ttactttatc tgtttgattc gaagcaaatt caccggtttt tttcgctgcg tcagttactt
     3181 tgtcttgcac cgtttcttga tctttttcaa attcttcttt tgttttgatg tccactacgt
     3241 tagcgttgac ttcgatcact tctaaatgtg tcatattttt tacttctttg ccgattactt
     3301 cttgcatttc ttcaaagatt ttttcgatat ctttgccata ttccacaatg acatccatat
     3361 caactgctac ttgtttttta ccgacttctg tttcgatccc agccgttaca ttatctgtat
     3421 tcacaagttt tcctgccaca ttagagaaga aaccaccgtt tgcagataat aaaccgtcta
     3481 cttgttcaat tgcgattccg atgatttttt gcacaacttt atcttcataa ttcagctcac
     3541 cgcgcattga atcttcctgt tcagaatcat gctttccttg gttatttgta tgatcagaac
     3601 caagttttcc ttgtccgttt gtgtggtcag aactaagttt ttcttgtgct ttcgcatggt
     3661 cagaaggatg tttttgctct tccacaggtt tagaaggatc gattgcatca tgttgttctg
     3721 gaactttaga ttctggagtt cctggatgat gaggtgcatg cttttctgca tcatgtttct
     3781 tctcttctac tccttgttga acgttaggga ctactggatc tggatgtgca gatactggtg
     3841 ctgttttgtt cttttcatcg ttcacaccat ttgattggtt tggtgtcgtt gggtctggat
     3901 gtgcagacac tggtttttgg ttgttcattt tattgttatt atttttgtta ttgttattca
     3961 tggatgtttc ctcctaagat ttaaaatagt gatctaagat tttcgtttgt ttcagataaa
     4021 aaccgatagc gacgccgatc gctgccatga tcaagatcaa taaggtcttg aaaaagccca
     4081 cggtgatcca taaaacggct aatatcaatc caagtacccc aaagatgata gggagcttgt
     4141 atgtcgaaaa tagttctttc atttttggtc cccccctatt ctactcgttt gcggtcattg
     4201 cttcgtttag ctggttcttc aaagcctgtg tatttcaccg atactttcac tggctctttt
     4261 gttcccaata tgtgttgtac ttcattttcg atttctttca tcaatgttcc tgttttacca
     4321 atgagtgctg aagttcgttt caactcccct tttacattga cggcgatctt gtttttcgtt
     4381 gctttgacat ttacttttgg tgaatggatg aattcatctt catgcaagtg agaacgaacc
     4441 attccttcaa ttgctttttt atccaacgtc aactccccat caccatgttt cagtagaaag
     4501 tttccgattg tttttggata aaacaggaaa acaagtaacg tgataattgt caaaatagca
     4561 aatgcaacgg caagccaaaa tacatattgt tgcacgtatg aattagtgat ggaaaagaat
     4621 cgaaacttct ctagctgaaa aggcaatgga taggttgcac ttaatagtat cgcaaacaaa
     4681 agcacagata gcaaaagtaa tgttccgatc actccgatcg tttttacacc tttattcatt
     4741 ttcgcacctc ctcatagggg tgaaacgatc aacttcttat aagttgatcg cttctccacc
     4801 agttactccg taaatttggg ctgtaacata acttgcttta ttcgatgcta agaaaacata
     4861 aactggtgct aattctcctg gctgacctgc acgttctagt aaactttgct gaccaaattc
     4921 agggatcgaa tcagaaggtt gtccttcgtc tagttgtaat ggtgtccaga ttggaccagg
     4981 tgctacacca tttacacgga ttcctttagg tgcaagctgt tttgctaaac cgatcgtaaa
     5041 gttagcgatt gaagcttttg ttgctgcgta atctaataaa tgttcgcttg gattgaatgc
     5101 ttgaacagaa gttgttgtta cgattgctcc tcctgctggg atatgcggaa cagcttcttt
     5161 cactaaagca aacatagcga tgatgtttat cataaatgtg tctttgactt gctcaagtgg
     5221 taactcagca atagactgac aagaaatttg ctgtcctgcg tttagtacca tcgtatctag
     5281 cccgccaagt tcactcacgg ctttatctat gatttcttta ggtgcttgtt cgtcacgaag
     5341 atcaaaaggt agtaagacaa tcttacgtcc tgcaactttc acgtattttg ctacttcctc
     5401 tgcatctttt tcttctcctg ggaagaattg aagagcgata tctgcccctt ctctagcaaa
     5461 tgcaatcgct gctgcacgac cgatccctga atctccacca gtgattagta cacggcggtt
     5521 ttctagttga ttattgcctt cataggattc ttctccacaa tcaggaacag gaatcatatt
     5581 ctgctgtaaa gctggtgtat cctgatcttg tttaggaaat ttctccgtat ggtacaagtt
     5641 tcgcgggtcg attaattttg gttctgtcat tgctgtattc ctcctagttt ttaaattgta
     5701 ttgccaaaac aaatttcttt gtttgatacc tgttttaaat agattcttta atttgtataa
     5761 tctttccggt ataaatggta acctaaaact actgctgcta tcaaagagcc aaagactacg
     5821 agtataaagc ctagagcaga acttttaccc ggtaatcctc ctgtattcat tccccaaatc
     5881 cctgcgaaca acgccggaac ggaaatgatt aatgcagctg aatctagata tttcatgata
     5941 tgatttagat tattgtccat catcccgttg aatagatcac caatactctc taataaatct
     6001 cgatagatct cgatcatttt ttctgtttgt cgctgtctca aatggacatc atagagtagc
     6061 tgttcatcgt ctaaacgttt gatgaaagtt tcagaattcc ataattgatc cattgtttcc
     6121 cgttgatctc ttaaggtatg atcgatatat acgatgtctc tttcaagatc tgcctgttta
     6181 tacagttctt cattttccgt tgttttacga gctgctttat ccaaagcgtc aattcgcttt
     6241 ttcatctctc ttagttcttt cacgtaatgg gtgtaaatat ctaataatgt atacgtgatg
     6301 atcttttcaa acgagtttaa atgatttcca tgctttgtca gcagccgatc cacaaattcg
     6361 gtttttctat cgatacaagt aatcaagaga ttatcggaaa gtacaaaaga tattggcgtt
     6421 aatttttttt cgatacgatt atctgactga tctagattaa tcactaccaa agaaaatgga
     6481 tgctctagtt tcgtctcata aaggcgctcc atccgagaaa cttcttccgg atattcggtt
     6541 cctacgaaaa tatcatcagg aagctcgaat tcttcgattg cttgctcaat ctcttcgggc
     6601 tttgcactat caagtacgag ccaattatat tttttttcat ctgtttctat tacattcttt
     6661 tcttcatcca caaagtagta tctcaatgta tacctccttt ttggactttc tcgaaactat
     6721 tagctgaaga agcagttctt taatttgata ttataatcga gtactgcgat ttcattttct
     6781 aatgaaaagc tgtcaagctg tttgttgaag tccatgttca atcgtttacc gaaataattt
     6841 tgctctaatt tagatgtata gatgtccaat aatggatgct cgattttagt cgcatattct
     6901 ccaaattccg aaacaaaagg aatgatttga aaaaagctgc cttttgcaaa agcacctttg
     6961 tcccttagaa atggatcgga atcattgata agcgcgagtg cgatttttgt attttcccca
     7021 accttttcaa ttaacgtctc tactgcattt ttagacattg taatttccat tcctactccc
     7081 cctcttcagt tgttgtcttc tattatttaa caactacatc tgtttttggt tgaacttttg
     7141 catattttgg atatttaggg aatttagcta cacaacggaa agccgtcatc cctgcatatt
     7201 tttcgtaata tttcttcgtt ggcccttcaa aatccttgct ccacggtttc acatgtcctg
     7261 aaaaatggat aatgtaagga ttgttgcgag tttcttcgta ttcacgttca cctgctgctg
     7321 ttggatgttt ttttgctttt gccatgatat agccttgtgc attccaacgc ggatgaagtg
     7381 gtaaccaacg gtcatgtaag atagcattta atgcatcttg gtcatggaat cgtaattttt
     7441 ctggattatg ttcgatgaaa tctaatactt tttgcgtaat attttcatct aaccattttt
     7501 tcacattgat cagcatcaaa cctgagttga aataacgcat tgatttagct gaaattttca
     7561 ttttttccag tcgttgatgg aacccagcgt cttcaacagc tgctacgatt gaatcaccga
     7621 agtcaagacg ccataattta gagatatctt gtaaagcgat catgtcgcaa tccatatata
     7681 agatacgttc aacttctgtg ccgcggaata attccggaat cgcaatacga taataagctg
     7741 tttctggaat acgatcgctg attaccacat ttgtaaagaa ttctttattg attttcaaga
     7801 attccacatc tgagttcatt cgtgcgtttt tcactgagaa gcgtaaaagc tgtttacttt
     7861 caaaatcgat atcatcgtca atcacataga accgaacaaa tttagacggg ttgcaattat
     7921 ctaatactga gacaaataat gcagctaaat gtggtacgaa ttttgtgtta caacttgcga
     7981 ctactgctat ttctttttta ttcattgatc tcgcctactt tcgttagagt actgtgtgct
     8041 aattttttta agtataaatt ggtatacgga tgatctttca acgtattcca aggtttatca
     8101 tgtcctgtaa agtgaacgat cgaaggtttt tcatttcctt ctttatactg acgttcatat
     8161 ttttcatttg gtgctggatg acgttcaaaa atcagcgaag tctgcatgtt ccattttgga
     8221 tgaagttgtt cccaatcttc ataaagtaca gcattcaaag catcttggtc gtgatagatg
     8281 attcgatcgc cattttcact gaggtaatgg atagtttttt ctgtaatttc ttttttattc
     8341 cattgatcaa tgtcaatcac catcactcct gaattgaagt acaagtcatc ggaatcgatt
     8401 cctaatcttt ccaaagcttt cacttggcct ggatcgataa tagcgccgat tgtttttcca
     8461 tttaacggct catcatatag ttttacgata tcgtctagta ctaacacatc tgaatctaag
     8521 taaaggactt tcttataatc ctctttagct aataagtttg gtaaagaaat tcttaagtaa
     8581 gcggtagttg tgatatggtc actgactagg aagtcttcgt agatatcttt ttccactgtc
     8641 aggaattgga tacttgcatt acttgaatat ttatttaccg tttcttccaa tccttcttta
     8701 ctatattctg atagaccatc gtcaatcaca taaaatttta tgcgtcttgt tttattgcaa
     8761 ttttctaatg cagttgcgat catcacgctc aagtaaggag cataattttc gtctgaagca
     8821 gttaccacag gaactacgcc gtatctcttc tccatcaaaa tcactccttt ttctaatctt
     8881 tttctaacct gttctacatc tagaatattc ctcatttggt tttaaagcaa aatatatgct
     8941 ttgttttcat tgaatatttt aaaatttttt tatcacaaaa cgcattggta agcgctaact
     9001 attcgttttt catggctttt tattcaaagg tattgaccaa aaactactat atatagccac
     9061 ctttttctta agaatgtttt ttgaaaaaat tcataatgac aaagcaagtt agtttcattt
     9121 ttttaaattc tcatttaatc ttaaggtaac aggaaaatag atatgtaaaa agaaagtagg
     9181 aagataaaat gaaactagca gcgattgatt tagacggtac cttgcttgat cgaaatggaa
     9241 agataccaga agaaaatatc caagcacttc ggaaatttga tgaaaatggc ggaatcgtaa
     9301 ccgtcgctac cggaaggaac agcatttctg ctaaagatat tttttccgaa ttaggaatcg
     9361 gtggctattt gatttcttcc aatggttcac tgattgctga aatgaaggat ggaaaaatgg
     9421 cgaaagttct cagacgttca aaaatagata gcgatacact gagaaaagcc ttcttctatg
     9481 ctcaagaagc gaaaatatcc atcattgcta gccgagaaac ccaagatgat cagctgacat
     9541 tcgatgaatc aaatctagtc aaagatgacc cttattatga acatttcaat ttacagaacc
     9601 attcttttga tcaaatttta aatatgttgg aagatccttc tcttagctat cttaaattag
     9661 cattaacaga taagagtgaa gaaaaactac agaagctaca aagagatctg caagcagatg
     9721 gtatccaatc agtattctct gatccgcatt tcttagaaat cacgcctaca ggtatcacta
     9781 aagctcattc acttgttttt ctggcggatt accttggaat cgaatcgagt gacatcatgg
     9841 cttttggcga tcaagaaaac gacattgcta tgttgaatta cagcgggttg agtattgcga
     9901 tggggaatgc acagcgacaa gtaaaagaaa ttgcaaacca agtaacactg acgaatgaag
     9961 aagctggtgt tgcttatatt ttaaaaaact tctattaaaa agaataaaat gacgatatga
    10021 atgtaggagg aaagaaaatg ttagttatta ttgatatgca aaatcatatt cttgatccaa
    10081 acagcgaatt ttatattcca gagtcagatc aattggcaga acgtattgct caaagattag
    10141 ctaaagcacg ccaaaataat gaatatattc tcttcacacg ggatatacca gttgaactga
    10201 aggacaaaga agaagaacga gaagatttgc aattgatccc tcttctttct ccacaagaaa
    10261 atgaacgagt gattaaaaag tattacttta cgattccacc tgaaacttta gctgaaatcc
    10321 aggaaacttt atttgaaagc aaagaagaac aaaaagagat cgaagtagtc gggattgaaa
    10381 caaacttgtg tgtactctct aatctgatag gcctgcagag tgcttttcct gaagcagact
    10441 tttttgttga tccaatgtta gtctccagta gaaaacacgg agaatccgct ttagagctgt
    10501 tgaaagattt caacgtctct atcgtcgaat cataaaaata tctatcttca tttacgatat
    10561 ttaacacaca ataaaaaaag cacgcagaac tgttttagtc ctacgtgctt tttctatcaa
    10621 agtagattgg cttttttatg acacagagtg aaacggcagg ctcacgacct ctttcttaaa
    10681 tggtgttcaa tcagctgacc ctcggtatta gcttaacaaa cggcaaaata tgaggaacta
    10741 tttttccgtt tgttaagcta atactcaggt caaaacgctg atttcacaac ctcttcctta
    10801 aacggtattc aaaagcttgc atctgcagta ataagaacag ccaagtaaaa acataagaag
    10861 ctgtttttac ttggctgttc ttattacttg atgcagggca gctttttcac aacctcttat
    10921 gtaccttgta ggactcgaac ctacgaccgg acggttatga gccgtctgct ctaaccaact
    10981 gagctaaagg tacatcataa ataaaattta tttttgttac ttttcaacta tagcacgaat
    11041 gacaaactct cacaagtttc aaaatataag taaaataata ttgttattct tatttctttt
    11101 acttgtgtca taactatttt gttactttaa tttttatttg cttattttag aaaaaatgtt
    11161 attcttttat ttattctcaa tgttaggagt gattattttg ccatttattg cgattttatt
    11221 cgatttagtt gcggcagcag cttattttct ccaattaaat tatcaaacct caggaattct
    11281 gtttattgga atgatttttc aaggtgtgat tacccttttg cttcttgtca tgatgctcag
    11341 ctataaagga aaaagatatg cccgtattca aacaaaagta tttgttaaat atgtttccat
    11401 tcgctatggg atcatcattc tttccttttt agtgaatgcg atcgtcctct ttttatatgt
    11461 attgaattac ttagatatca atccattgat tttttctcga tagataagac acattcagcg
    11521 gaacagttcc tgcttatcag cagaaaactg ttctgctttt tcatttttca ttgatctatt
    11581 cttcttttat tttcctagca tttcatttgt gatgtatgca ataacatgtt atcttttatt
    11641 atatgaatcg ttgaggagtc cgttacatga ttacgttggt gagagttttg ttttgggtgc
    11701 ctgcggtcgc actggtcgca agtattgttt atcttatgaa ttggaacaaa gaacggtttt
    11761 atttagctat tttgactctt ccggctatct actttatgtg gaaagttttt aattacaact
    11821 actttgaacc cgatagtgtt tttatagaag aattatcagg actggttttg tctttattga
    11881 tcgtcattct ttatttgata cgtttaaata aaaaacattg agtactattg attgcatgtg
    11941 tagctttcta tagtcattat aactattcta ctataatgaa aggtgattaa atgagtatcg
    12001 aagaagctat tttactggat actttacgtt gtcgtctgaa tttcttaaga actggccgat
    12061 ctttatctca agtagctgtt tctagcgcgt taagtattcc tttattcaaa tatcgtaagt
    12121 acgaagaagg agaagaaatt ccaagcatca ttgaattaat ctatatcgct gacttttacg
    12181 atatttccgt tgattaccta attggtcgct cagaaacagc aagcaaactg aagtgatttt
    12241 ttccagcctt ggtgctggtt ttttcattta acgtctaagg ttaacaatct atatataaag
    12301 gagacatttc tatgcgatta tggcatgaaa aattgatttc aaagcttcct cgtcaacagc
    12361 ttttaggaca acatcgggaa tgctgtgcat taagaggaaa tggctgggga aagaaacatg
    12421 ctactgtgga ttatgttttt cactattctc cttatagatt attccaatat catcagcttg
    12481 tcatggatga aatggaaaaa cgtggttaca gcccagcacc agagtggaaa aatcctatgt
    12541 atcgtggcaa atcatgcgaa gcattttttg atttacctac tgtagagcgc aacgtgccga
    12601 tttatccgga acacgatgaa gtttatctag cagaatgttt agcaaatttg gagcaaaaag
    12661 gaatttatct tacctaatat aactagaaaa gccttctatc tcctcttact tcattaaaaa
    12721 gctttaacca tcttagatca agctaaaatg tcgtaagttt tagggttatc ttcatcctca
    12781 cgtgcaaaac taaaagcgta ctctagtttt ggttcatcgc caacttccct agacataaat
    12841 gaacatctcc tgacgtggtt taaaagtacc gcatcttcga cgtctgtata cgatgcacag
    12901 cacagattgc ttcttttgat ctttcttttg atttttctgt tgcttcttct acaccttctt
    12961 cgattactac atcgatgctt acaattgctt ctgcctaata ttcccatgca tcgtcgattt
    13021 aagcctctgt agtcactgtg atcgtttcca cattttaact cctctgtcgt ggcttcagtt
    13081 gattttgtga tcagttcagt tgctaagcca gagtcagaac aacctgccat tgctagcgtt
    13141 aatattgata gtaaaaatag tttttttatc ataaggttcc ccccctgaaa atagactaaa
    13201 ataataacgc tttctttttt tgttttcaga cgaaaattaa taatttctaa atattcttta
    13261 aaaaagctta taactattat gcctttttta atatataaca cagt
//
LOCUS       DAEGIQ010000074        13294 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000074 DAEGIQ010000000
VERSION     DAEGIQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.075"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            312..2192
                     /locus_tag="KMT45_001933"
     CDS             312..2192
                     /locus_tag="KMT45_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005389.1"
                     /translation="MDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNL
                     FWQKKNKGIEMVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFT
                     TQTVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMI
                     RNLRIGLVAVSLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQS
                     VSRIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYT
                     ALVQIDLLEDSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDA
                     EASKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGP
                     SSIIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNP
                     LSREVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLP
                     ITECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGE
                     KFGDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKK
                     YL"
     gene            complement(2774..3646)
                     /locus_tag="KMT45_001934"
     CDS             complement(2774..3646)
                     /locus_tag="KMT45_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7005390.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            3946..4920
                     /locus_tag="KMT45_001935"
     CDS             3946..4920
                     /locus_tag="KMT45_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7005391.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            4942..5634
                     /locus_tag="KMT45_001936"
     CDS             4942..5634
                     /locus_tag="KMT45_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBE7005392.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            5600..7108
                     /locus_tag="KMT45_001937"
     CDS             5600..7108
                     /locus_tag="KMT45_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBE7005393.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            7182..8285
                     /locus_tag="KMT45_001938"
     CDS             7182..8285
                     /locus_tag="KMT45_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBE7005394.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            8288..9625
                     /locus_tag="KMT45_001939"
     CDS             8288..9625
                     /locus_tag="KMT45_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBE7005395.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            9612..10490
                     /locus_tag="KMT45_001940"
     CDS             9612..10490
                     /locus_tag="KMT45_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBE7005396.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            10487..11410
                     /locus_tag="KMT45_001941"
     CDS             10487..11410
                     /locus_tag="KMT45_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBE7005397.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            11407..11688
                     /locus_tag="KMT45_001942"
     CDS             11407..11688
                     /locus_tag="KMT45_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBE7005398.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            11692..13260
                     /locus_tag="KMT45_001943"
     CDS             11692..13260
                     /locus_tag="KMT45_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBE7005399.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
BASE COUNT         4450 a         2197 c         2813 g         3834 t
ORIGIN      
        1 aaagtacagt attttataaa atatttttac cgatgttttt attaggagcc gtacttgtca
       61 taggatttag tttattcatt taccagaata cctatgaatc gatcgaatcg agttatttaa
      121 cagacaaaaa gaatttgctc aggcaagtta aaactaacgt tgaatggaaa attagaacga
      181 ttgaatattc gttttctacg tatggttcga ccaaaaattt ctcggacata ttcaagcatc
      241 cgctgacata tacagattac tccacttatt ctgaaatcag gaaagaacta aattttatag
      301 aaacgattgt tatggatgat aataattatg atttagtcag tttggctggt aaatggggag
      361 taatcaatgg atcgttgagt caactgaccg aagaggaagt tgaaaagtat caacagaagt
      421 acataaataa caaaaataat ttattttggc aaaagaaaaa caaaggaatc gaaatggtga
      481 tttctttgcc aatgtttgaa aaagaaaaat atgcattggg aattgctacg atagagaaac
      541 atacaataga acaagttgtt ggtgaaagtg gagaagacat actttctatt gaaaacgaag
      601 ttggcaacct ttttactact cagacagtaa aaaataaaga gcaaccaact gtattaacgg
      661 acgtggatct agattacaat cagctgaaag tgataagaga aaaagataaa acgtatatag
      721 tactgaaatc agattataat aattggattt acagattaga agttgactct agtgcaattg
      781 gcgcaatgat ccgcaatctg agaatcgggc tagttgcagt gtctttaaca ctggtaggac
      841 tcattggtct tttttcctac ttattttcgg acagatttgc ccgtccgatt tctcaaatac
      901 aggaacgtct gaatcttaaa tcaaaaggat tcagtgggaa agaactagat ttggtcgctc
      961 aatcagtcag tcggatcata ggagaaaaag aagcgttaag tgcacactta gttacccaaa
     1021 agccacagct ggaaacgcta tttgtactta gtttatttag aaatcgagta gaaaggcgag
     1081 aactagatca gcggttgcag caatttggtt atagttctca aaatgactgc tattatacag
     1141 ctttggttca aatcgatctg ttagaagata gtcacggtgg ggagcgtgat ttgctacttt
     1201 tagcgatcaa caacatgatc acggaaattg tgccaaagga aaatcgaatg ctgccgatcg
     1261 ttttgaatga agaaatgcaa gccactattt atcgaatgga taaagatgat gcagaagcaa
     1321 gcaaaaaagt catggagtat tgccgactca ttcaaaaacg tgtaaaagaa tacttgaatt
     1381 taactatcag tataggcatc agtaaccggt tcgaaacgct gaatgaaagc aagcaatcgg
     1441 ttgatcgggc aaaagaagcc ctgtattatc gtgtaaatac tggtccaagt tcaattattt
     1501 tttatcagga aatcgttcct gctgctcatg aaaaaacact tattagatat ccgatagaag
     1561 aacaaaatcg attgtttgaa gccattcgtt ctggaaatca ggaagtgaag aatttagtac
     1621 atgaattgat tgatgcattg tttgcacaaa ataaaaatcc tttgagtcgg gaagtagttt
     1681 cggtccgctt gatcaatgaa ctcgttcaat taggacagtt attgggagta gattcaaaaa
     1741 actttgataa catgaaacaa atttatgtaa aagcactgaa caattatcat cccaaagaac
     1801 tagaaaaact tgttttagaa caattagttc tgccaatcac agaatgctct caagcaatca
     1861 ctgatcatga atttaaaaat ttgtcggaga atattatgca tatcgtctac acagaatatg
     1921 atcgtgatct gtctttagat gtgatcgctg ataggttgca ttataaccct aactatttaa
     1981 gtaatgtatt taaaaaggaa acaggagaaa aatttggtga ttttgttcaa aatcatcgtt
     2041 tagaggtagc aaaaaaatgg ttgaaagaga ctaacctctc cgtaaaagag attgcagaaa
     2101 gattgcaata tcgaaatccg caaaatttta ttcgtttctt taaaaagaag gaatcgatta
     2161 cgccgggaga atatcgaaaa aaatatttgt gaatcaagag gctgtttttc ttgtgatctg
     2221 attcaagcaa taaaagagga cagcaaaaaa cagcacttca tgttttgctg ctggctgatt
     2281 ttattgccgc agaatcaaca agataaacgc cgtttaatca agaggctgtt tttcttgtgg
     2341 actgattcaa gcaataaaag aagttagaat aaccctagta tttgtattta tggatagtta
     2401 aatttcaagg ctaagcacgc aaaaagaact gtgacaaaag tcttgtcaca gttctttttt
     2461 acgaataaat ggcgttcaag agacaggcta tcgacaataa ggcataattt tgtgaaatat
     2521 gggaagctat ttttcaaaat cgcttcttat tgctcctgtg ggttactcgt cgcaatgttt
     2581 gtgagagttt ttatcatcgc tcaaggctga acgtctcttt cacaacctct gatacacggt
     2641 gttccaggag tagattcttc gaaaaataag ccgaaatttc acaaaaattt aagaagcaat
     2701 tttcgtaaat gccttcttat ttcttgaagc tgaccacttc tgacacaact accattttgc
     2761 gttcagtaca taatcaccct ttgacagaac ctacgagtgc tcctttgaca aaatatttct
     2821 gaacaaaagg ataaacaatc agcataggca ctgttgcaat aacgataaca gccattttaa
     2881 ctgtttgtgc tggtggaatc acatcaacgg cactactttc tgcttgcatc ccacttgata
     2941 caatgacgat ttggcgtaaa agaatctgta tcggccattt agatgaatcg ttgatataca
     3001 aaatagcagt catataagtg ttccagtaag taacagcata aaaaagcgag atagtagcaa
     3061 tagacggtaa agctaatggc agcataattt tgaaaaatac accaaaatct gtgcaaccat
     3121 ccatttttgc tgattcttcc aaactatccg gcaaggcttg gaaaaagttt ctcataatga
     3181 tcatattcat tgcattaaca gccacaggca ggatcatggc ccagtaagaa ttgaccagcc
     3241 ctagagaacg aacaactaag aacgttggaa tcatcccccc gctgaacaac atagaaaata
     3301 caacaagaaa gttgacgaaa cctcgaccaa acaaatactt tctggaaaga ccgtaggcca
     3361 tcagtgaagt tgcacacata ctgacgatcg ttccgacacc tgtaacacca atcgaaacag
     3421 caagtgctct aaaaatggtc ggcgtagaaa gaatgtagcg atacgcgtct aaagaaaagg
     3481 tagtaggaaa aaggataaac tttttcgcga cgacttcttg tgtagaagca aaagatgtag
     3541 cgatgatatt taaaaaagga acgatacaga tcaaagctag caaaattaga aacgtattgt
     3601 tgaaaagtgt aaaacttctt tctcctatag agactttctt tttcatatag ataggctcct
     3661 cctcaaaata aattaaatgt ttctgtttct atatgttcaa tttatcatcg gtgaaagatt
     3721 acgtatataa tcatttccta tgctaagtgt cagatagaaa aaaatagaag gtttgctatg
     3781 cgtagtttat ttgtgaaaaa atacacttta tcgttttgta tagcacaaaa atcattgcta
     3841 tgtaagcgtt tttacttacg ataaaaatga ttatatacgt aattcagata gttatctatc
     3901 atgaagtcat aagcagcaaa cattcacaaa actaaggagg ggcttatgga aagtgtaaag
     3961 aaacaagaac catttcaact gacgctatcg aaaaaagaag aaaagctcag aagacgaaaa
     4021 aaaataattg caggaatcaa aacgaataaa tttttatatg tgatgattct gccgggagtc
     4081 ctttatttct tgatttttag gtatcttcct atggggggac tcgttattgc ttttcaagat
     4141 tatcaaccat ttttaggaat aatgggaagt ccttgggtcg gtttcaaaca tttcattcgt
     4201 ttgtttacag aaccgacatt ctttacatta ttaagaaata cgttgatctt gttcggatta
     4261 aatatcttcg tgttttttcc gatgccaatc attttgtctt tactattaaa tgaagtaacg
     4321 aacaaacgat tgaaaaacgg gattcaaacg atcatttaca tccctcactt catgtcttgg
     4381 gtaatcattg tttcgattac ttatgtattt ttgaatgttg acggaggggt attgaatgaa
     4441 ttccttgcaa caatgggatt tgaaaaaatc agttttctaa catcccctga atggacacgc
     4501 acagtatata tcggacaagt gatctggaaa gagttgggct ggtctacgat catctacctt
     4561 tcagcgataa ctgcagtcga tacacagttg tatgaagctg cagaaatgga tggagcagga
     4621 cgcttacgca aaacatggca tgttacattg ccggcaattc gtccagtaat cattaccttg
     4681 ctgattctaa aaatcggtca gacattagac ttagggttcg agcatatgta cttgttattg
     4741 aattcattga atcgaagcgt tgcagaaatt ttcgacacct atatttatac agctggattg
     4801 aagaatggac agttaagttt cagtacagca atgggattgt ttaaaggggt cgtaggattg
     4861 tttatggtaa ttttagctaa taagattgcc aaaaagctag gagaagacgg cgtttactaa
     4921 gatagaaaga gagggaaaga tatgctgcag tcattagata aaataaacgg aaggctaaat
     4981 cgtattttac agttggttta tttgaatttt ctgtggggcg gtttagtcat cgcaggtttg
     5041 ggagttttta ctttcggtcc tgcatcgtat gcgatggttg cagtgattag aaaatggctg
     5101 cgctcaaaag aaacgtttcc tttgacgaaa acgtacttca catattttaa agaaaactat
     5161 cgtgaaacct tgctgatgag ctggatatat ggcgccatcg ggattgtttt acttgtggat
     5221 ctttctttca tacacaattg gtatcttagg atcgctttgt atattgctgc gttcatatat
     5281 gtgctctcag cactctatat tttcccaatc atggctcatt atgattggaa aggatatttt
     5341 ttcaaaatga aaatggcatt tttattcgga ttctcacatc ttcattactc attagtgttg
     5401 atattaagta tggtagggat ttatctgatg atcatccaaa caatgccagg tgttttaacc
     5461 tttatcggtg gcagtctctt cttttccgca gtcacgtgga cagcaaattc tatattttgc
     5521 catttggaat ggaagaactc atatacagaa aaaacagaag aaaacaaaaa tattattaaa
     5581 acagtgaagg agaaaattta tgaaaaaagc aacgctcgta aaattaggtc ttagttttgc
     5641 aggaattcta gcacttgccg catgcggcag cagttcaagt aacagttctt cagagacaga
     5701 agatggaaaa gctaaaatca cttggttaaa tattttgcat catgcttcac cgcctacaga
     5761 cacgattgtg aacttgatcg aagaaaaaac cgatacggaa ctgacattca actggattcc
     5821 tgatgcttca aaagaagaaa gattaacaac tgcattggct tctgatgaac taggagatat
     5881 cgttacactt caaattgcga tgttgaaaaa ctcttctgtc cgcaatgcgt taaaatcagg
     5941 cgtattctgg gatgtatcag aatatttaaa agactataaa aatttaagtc aaatttctga
     6001 agatagaatc gaagctgcga gtatcgacgg tgccttgtac ggcgtaccga tacaaaaaga
     6061 ctacgctcgt gctggcttag tcattcgaaa agactggttg gataatctag gactagatgt
     6121 tccaactacc atggatgagc tttatgaagt agctaaacaa tttacagaaa acgatcctga
     6181 tggaaatggt gtggacgaca cagttggttt tggcgatcgt gcgcctagtg atattcgtta
     6241 taccagcttt aaacttttca cttcttattt tggagcacca aatggctggg ctgtagacga
     6301 taatggcaaa ttcactccag aatttgatac agaagagtat atgaaagctt tgaatttttc
     6361 aagagatcta tttgagaatg gctatttggc acaagacttt gcagttaccc aaaagactga
     6421 tcagcaagaa caatttgcac aaggaagaac tggaatctac actggaatgg tggatatcaa
     6481 gaatctgaag acgatgggtc aaggtattca agatgatatg gaattagtgc ctgtaaacaa
     6541 gatctcagat ggtgaaggcg gcggttatca tgtttggtca gaaggaaatg gtattggcgg
     6601 cttgctagca tttcccaaat ctcaagtaaa agacgaagca caattgaaga aattacttca
     6661 atttgttgat gatcttttag aagaagatgt gtataaattg atgactggcg gtatcgaagg
     6721 aacgcattat gaattgaatg atgatggttc tatcgaattt atcgatatgg atctttggca
     6781 acaagaggtg caaccgttat caagctctcg tcctagtgaa gtgacgttct ctttcaaaga
     6841 tacagaccct gaaaaagaat tctctaatca attgatcaaa gaaaatgaag aatttgctgt
     6901 aatggatcct tctgtgccat tggattcacc gacgaacaat gaagtaggat cagaaattga
     6961 aaaaatcgtc attgatgcta cggtgcaata catcatggga cagcttgacg aagatggatt
     7021 caaaaaagct gtagaagact ggaaagcaca aggaggcgat cgaatcacac aagagtacga
     7081 ggaagcatac aaagcagcgg cacagtaata tagacgattg ggacaaatac ttcttggtat
     7141 ttgtcccaaa tcctatttta atttttaaag gagagcaaat aatggaaaac tggtcaaaga
     7201 aattagcaaa aagtatcatg gaaagaacac ctcgcttata tgaagaaaag tggtataaag
     7261 gaaaatggtc ttatgactat ggcgtcgtac ttaaagggtt ccaattactt tgggaacgaa
     7321 cccaagaaaa aatttatttt gatttcataa aagacaatat cgactatttc gtgcaagaag
     7381 atggaacgat ccgtggctac agcgttgaag aatacaatat cgaccatgta aatacaggaa
     7441 agcttttctt tttattgtac aaagaaacag gcgaagaaaa atataaaaaa gcagcagaac
     7501 tcctttccca gcagttggca aaccatccac gcacatccga aggagctttt tggcataaag
     7561 aaatttatcc ttatcaaatt tggttggatg gcttatatat gggttctcct ttttatgctg
     7621 aatttatcca gaattttagt gaagaaaaag attaccaaga tgttttgaga cagtttgaaa
     7681 tcagttataa acatctattc gatccaaaaa caaatttgct gattcatgca tgggatgaaa
     7741 agaaggtcca accatgggct gatccaaaaa caggtctatc ccatcatttt tggagccgat
     7801 cgattggctg gtatttgatg gctgcagcag atacctatga attacttcat gaagaaacag
     7861 attgttcttt gctgaaagaa attctagaaa agacactggc tgcattactt ccatatcaag
     7921 aagaaaacag cgggacatgg tatcaagtaa caacagaaac aaaacggaaa ggaaattatc
     7981 tggaagcttc cggcagtagt atgtttgttt acgcaatggc taaaggaata cgtttgggac
     8041 tgctgaacaa agaagaatgg cttccacaag tgaagaaatc acatcaagga ttacttgatg
     8101 aatttgttct ggaaacaaaa gaaggctggc tgaacttgaa taaaaactgt caagtagcag
     8161 ggttaggcgg agcagatcag cgagatggtt catatgctta ctacatcagt gaaccaatta
     8221 tttgtaatga tcaaaaaggc gttggtgcat tcttgcaagc attgattgaa gtggaggcat
     8281 tataaatatg tatgatattt tgacatttgg cgcatcaatt gatgaattga atactgaagc
     8341 aatacaacaa gccattgatg ctgcagcaag tgacggaggc gggaccgtag ttgttccagc
     8401 aggtgaattt ctaacaggag ctttattttt aaaaagcaat gtcgaacttc atttaagcgc
     8461 aggagcggtt ctgaaattct ctgacgatcc aaaagattat cctgttgttc attcacgttg
     8521 ggaaggagtc catcgaaaag tttatgcttc ctgtatctat gctcaaaatg tagagaacat
     8581 ctctgtcact ggtttcggta cattagatgg aaatggaaaa aaatggtggc atacttttag
     8641 aaatgaacca gataatctcg cttatcctcg tcctaaattg atgagtttcc ataactgtca
     8701 tcgaattact gtgaaagata ttaaattgat ccagtcacca agttggacga tcaacccgat
     8761 tctttgcagt aatgcaacgt ttgataactt gacgatcttg aacccagcgg attcaccaaa
     8821 tacagacgga attgatccag aatcatgcaa aaatgtgcgt attagtaatt gccatattga
     8881 cgtgggagat gactgtattg caatcaaggc agggactgaa gatacgtacg aaaggatcgc
     8941 ttgcgaaaat atcacgatca caaattgcac gatggtccat ggacatggcg gtgtcgtatt
     9001 aggaagtgaa atgagtggta gtattcgtaa tattaccatt tcaaattgta tcttccaaga
     9061 aactgatcga ggaatacgat tgaaatctag acgaggtcga ggcgggatcg ttgaagatat
     9121 tcgagtatcc aatatagtca tggataatgt gatgtgtcca tttatactga atctttatta
     9181 tttctgtggt ccgcgaggga aagaaccgta tgtatgggaa aaaaaggctt atccgataga
     9241 tgaacggaca tcagcattca gaaggatcca tttttcgaat atcacggcaa ggaatgtcca
     9301 tgcttctgca ggttttattt atggattagc ggaacaattc atacaagaaa tcacatttga
     9361 caatatcgat gtctcgatgg cccaagatgc agttcccggt gttcctgcta tgatgacagg
     9421 aatagaggaa atgagcagac aaggatttta tattggcttt gcgggtaagg tttcctttaa
     9481 ccagatgtcg attgaaaatc acgagggacc agcatttcta ttagaaaata ctaaagaaat
     9541 cagtattgag aaattccaat caaatcaatc agaaaatatg gatgaactgg ttgttcgtaa
     9601 aaactttgct tatgcagttg aatgattttt taggtgctcc atctagcaga cataccccta
     9661 gtggaaaacg attataccga gaaaaacaaa acggttttat gcttgaatac tatcaattgt
     9721 acttaaatga attggaaaca gtaccagccc tctatgctta tccagaaaaa gaaggaccgt
     9781 ttccgacggt cctttacctg cattctcacg gcggagattt ttcagtcggg aaaagtgaat
     9841 tgatccatgg tgctccttac ttagcttctc catcttttgc agaagtactg acaggaatgg
     9901 gatatgctgt ttggtcgatt gatgcttggg gatttgaaga acgcggaggt atttctgaaa
     9961 gcgagttgtt caaacaattt ttattaacag gaaaaacgtt atggggaatg cgtatttatg
    10021 atgtgatcag cttgatcgat tatttggaga caagggaaga cacagatact caacggctcg
    10081 caacgattgg tctttctatg ggaggcttat taagctggtg ggtgacagca ttggattctc
    10141 gtgtcaaagt ttgtattgat ctagcggcac aggtggacat cgaaacactt ttgctacatc
    10201 gccgcctaga tcatcatggg ttttattact atgtgccaaa tctattgaca gcttatacaa
    10261 cgttagcaat ccaagaaaaa atcgctccgc gccctcgtct tagcttagtt ggaaaaaacg
    10321 atacgatgtg cttggacgaa ggcgtactga agctgagaaa agggctggac aaaaagtatc
    10381 aatcaatggg tagtccagaa aacttttctt ctttttcttt aacaggcggt catatggaaa
    10441 cacaagaaat gagaaatatt tggaaacaat tcataaggga gcatttatga ggatgaaaga
    10501 caaactgata attggaaaga atgctataga taccccgacg gattttgaaa ctatccaaga
    10561 aggaatcgat tatttggcag gcctgccgga tagccatcca aaggtcttga tagtaaaaga
    10621 aggaagatat gatgaatata tagaaagcag attatccaat tttaaacttg tgggagcagg
    10681 aacagtagtt atctccggta atcgttttgc aaaacaggag tatcttgacg gtagccagtt
    10741 agggactttc cgaagtgcta cttttttctt agaaggatcg aatgtcgtat tagaaaatct
    10801 tcacatcgaa aatacagcag gtccaggaga aaaggtagga caggctgttg ctttattcaa
    10861 ttcaggagat cgcgtatctt taaaaaactg tcgactatct ggttatcagg ataccttatg
    10921 cacgggtccg ttgcctgatt tgcaaaaaga tggtactcct ttttgtacac cggcttctgc
    10981 caatcctgag tattgtcgtc agacctatga atattgctgg attgaaggaa cggtggattt
    11041 catatttggt ggggcagatg ccgtttttca tcagtgtcat atccatagct tagccagtaa
    11101 aaatcctgga tacatcacag cagcatctac gccgaaaaat caaaaaacag ggtatcgttt
    11161 tgaaagctgt accttgactg cagaacctga tactagaaac gtctatttag gaagaccgtg
    11221 gcgtccgtat gcaaatgtct tatttgaagc gtgtaagata ggtgggcatg ttcacccaac
    11281 tggttgggat aattgggatg acaagaaaaa tgaacagact gtcaaattct ctgaacgtaa
    11341 taacaattat gaaacatcga tcaaaagacc tgaatggata catttagaaa agcagggggt
    11401 tcagaaatga aaagaaaatg ggaactattg ctaggaatgg tcggtggcag tctatctttg
    11461 attttctttg gcgggttagc agttacttta agtaatatgt ctgcgtcaga gtttaaaaaa
    11521 agttatcagt cgcttgctgt agatcattca actttgtctt tagaaaatac atttggatta
    11581 ttacaggaca tgactggtct gtttgccgtt gtgttgttta tttcacttgc ttttttagca
    11641 gctggggcat tcagtttata tcgagtaaga atgagaaaag gagcctgaat catgcagact
    11701 atcaaagtga aaaatcctat tttaagagga ttcaatccag atcctaatat attaagagtg
    11761 aaagagcggt attacattat tgtctcctct tttgaatggc ttccaggaat ccgagtatat
    11821 gagtcgaaaa atttagtcaa ctggtcacat acaacggata ttttgaccac tcaagtatct
    11881 ttgcaaggaa atcctacaaa tggaagtata tgggcgccac agatcagtta tgctgatgga
    11941 ttgttttatc ttgtttacac agatgtcaaa agtgcaggcc gtccgtttaa agatgtccac
    12001 aattatttga tcactgcatc tgagatcaca ggtccttggt ccgaaccaat ttatttaaat
    12061 agtaacggct ttgatccttc tttgttccat gatcttgaaa cgggaaaaaa atggcttgtc
    12121 aatgaactat gggattatcg tctggacaca ccgaataaat ccagcggtat cgtattacaa
    12181 gaatatgatg cagagaagaa gaagcttgat ggtcccatct ataaaatatt tgcaggtaca
    12241 gaattagcga aaacggaggc accacatttg tattttcgta acgggtatta ttatttgatg
    12301 acggcagaag gcggtacagg aagaggacat tcagttacag tttgccgagc aaagaatatt
    12361 actggtcctt acgagcttga tcctggtttt cctatgctga cagctagtga caaacctgct
    12421 tctacattgc aatgtacagg acatgggagc ttagtacaaa ccccgtcagg aagatggtac
    12481 atgacatatc tttgtaccag accattaaac ggtgcggcta ttttgggaag agaaaccgct
    12541 attcaagaag tgtattggac gtccgacgat tggttaagac ttaaagatag ccagacaact
    12601 ccgatggaag aaataatgat tgagacaaat gaagaggtac aacaagaaaa agatcatcag
    12661 tttatggata catttgaagg agagctaaag aagaactgga atgccagacg cattttgcca
    12721 aatgctgatt ggtgtgatac aaaatgtaga ccaggttttt tacgtctgat ttccggtgaa
    12781 tctcttcaat ctaccttcga acatcatttg ttagctattc ggcaaacgga ttttactttt
    12841 gatgcggaga cagcatttga atacatgcct aataatttta atcagatggc tggactggta
    12901 ctgtacttaa atgaatccaa ttatatttat tgttatgtta cttggaatga ggacaaggga
    12961 aaatgcctac gtatgataga atcagaaaac ggagtagcac aaatagaaga ttggacgatt
    13021 ccactcacag aaaagaagac ttttttgaaa gtcactgtaa agaaagagac agcacagttt
    13081 catttgaaag acgtatcaac tgaagaatgg aagaaagtag gtccgcaaaa aaacatgtgt
    13141 atactctcag gagggtttac tggaaatttt atcgggatag cagtgcatga tctaaataaa
    13201 aaaagaggaa gttatgctga ttttgagttt ttcaagtatg atggaaaaga tcatctgtaa
    13261 tttaattctt taataaatga ggttcactaa ctta
//
LOCUS       DAEGIQ010000075        13276 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000075 DAEGIQ010000000
VERSION     DAEGIQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.076"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            135..1454
                     /locus_tag="KMT45_001944"
     CDS             135..1454
                     /locus_tag="KMT45_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBE7005400.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1895..3535
                     /locus_tag="KMT45_001945"
     CDS             1895..3535
                     /locus_tag="KMT45_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBE7005401.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3689..5011
                     /locus_tag="KMT45_001946"
     CDS             3689..5011
                     /locus_tag="KMT45_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBE7005402.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5073..5504)
                     /locus_tag="KMT45_001947"
     CDS             complement(5073..5504)
                     /locus_tag="KMT45_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7005403.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5568..6404)
                     /locus_tag="KMT45_001948"
     CDS             complement(5568..6404)
                     /locus_tag="KMT45_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBE7005404.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(6594..6896)
                     /locus_tag="KMT45_001949"
     CDS             complement(6594..6896)
                     /locus_tag="KMT45_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBE7005405.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(6922..7353)
                     /gene="ruvX"
                     /locus_tag="KMT45_001950"
     CDS             complement(6922..7353)
                     /gene="ruvX"
                     /locus_tag="KMT45_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBE7005406.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(7350..7616)
                     /locus_tag="KMT45_001951"
     CDS             complement(7350..7616)
                     /locus_tag="KMT45_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBE7005407.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7870..8595)
                     /locus_tag="KMT45_001952"
     CDS             complement(7870..8595)
                     /locus_tag="KMT45_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBE7005408.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8961..9031)
                     /locus_tag="KMT45_001953"
     tRNA            complement(8961..9031)
                     /locus_tag="KMT45_001953"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8997..8999),aa:Gly,seq:ccc)
     gene            9148..9900
                     /locus_tag="KMT45_001954"
     CDS             9148..9900
                     /locus_tag="KMT45_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBE7005409.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9916..10395
                     /locus_tag="KMT45_001955"
     CDS             9916..10395
                     /locus_tag="KMT45_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBE7005410.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10646..12226)
                     /locus_tag="KMT45_001956"
     CDS             complement(10646..12226)
                     /locus_tag="KMT45_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBE7005411.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(12219..12962)
                     /locus_tag="KMT45_001957"
     CDS             complement(12219..12962)
                     /locus_tag="KMT45_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005412.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(13138..>13276)
                     /locus_tag="KMT45_001958"
     CDS             complement(13138..>13276)
                     /locus_tag="KMT45_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBE7005413.1"
                     /translation="TENITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENES
                     F"
BASE COUNT         4063 a         2644 c         2367 g         4202 t
ORIGIN      
        1 attaaaacca tgtgaaactg cctcacggca gcttacaatt actttttcat cttcacccgt
       61 taagtgatga aaaaagaacc cctttctgct atggttaaag tgactaaacc aaccaaaaga
      121 aaggagttct tttcatgact agcttacaca aaaaccaagt aaaattcaat tcaaatatca
      181 ctatttctca tacaggtggt caattatcga gtgattcggg tctcgtccta gtgaaagagc
      241 tgatgaacat ctttggtttt tctgaactgg ctaagcaaca cattcacatt gaagacgaac
      301 gagcgtattt tactcatgac aacttatcca tacttgagca gttcattatg caattaattg
      361 ctggttactc agctgattct gcagctaatc tcctgagaca agatcctgtg tttaaagctg
      421 ttttagatag gaaagaactc gcttctcaat cttcactttc ccgattttta gatcgactat
      481 ctgaggagaa tatccatgaa cttcaagctt tgaaccaaga acttatcgat aaagcacgcc
      541 tcatccgtaa tgatacggaa ttaatcattg atttagattc gacccattca gatacatttg
      601 gtcaccaaga acaaacgaat tataatacac actaccaaac ctatggttac catccattgg
      661 tagcttttga cggattgact ggtgacttct taaaagctga actacgttca ggaaatcaat
      721 atacatcaaa aggcgtaaag gagtttctca cacctttatt agagcactat aatcactctc
      781 taccaaacac tgacatcttg gttcgtggag acagcgggtt cgctacacct ggtgtgtatg
      841 attcgtgtga atcaaaaaag agtcattatg ttattcgact gaagaataat cgtagactag
      901 gtcaaatagc tgagaaatca gtactttatg gcgataatca aaagtgggaa gaacgagaag
      961 ttcagtactt ctcgaccact tatcaagcac aatcctggtc acaaagtcgt cgcgtgtgta
     1021 tacgctcaac acgtgaagcg ggcgaactac tctttcgaca tgaatttatc gtgacgaatc
     1081 tatcagaaaa tgtttctcct gataccatct tttctatcta tgccaaacgt ggcacaatgg
     1141 agaacttcat taaagaagcg aaagctggct tttactttga caaaacagac agtcctcgct
     1201 ttttggagaa tcatgtccga atgatgctca gcttgatagc ttacaactta gtcaacttct
     1261 taagaacgat tggctttgaa gaagtccaaa agggaatgac cattcattct attcgattga
     1321 agtttctgaa agttgctggg aaattagtcc aaacgggtag acgagtctat ctcaaattat
     1381 ctagttatca tgtgtatcag aatgaattct acagggtctt tgctcgcctg aggcgagcca
     1441 gtcaatggat ctaatccaac aatctaacga tttttttact gggaagttat ccagggagaa
     1501 gtatgctcaa aatgccttac acccaaaatt tattcctcaa tatttttgaa cagtgaatgt
     1561 ttgaagtaac tttttagtca aaatcagttg atagggatat tagtgataca tatttcaata
     1621 aaatgtgcca aagaattaaa gctatgaatt attcaggatt ctatttatct atctaacata
     1681 tcctttattc ttctactttt ctttctttag catataagag atacgcatag taatcaagca
     1741 aggattgttg atataaaaag aacaaatacc gctattttga tttcttttga ctctattact
     1801 catataaata aaatttatta tacctgctct ttttgactat atcctcatct gactcctata
     1861 cttcgtttgt attaaaactt aggaggaatc attcatgctt acagggctaa acttattaaa
     1921 taatcctttt ctaaataaag gaacagcttt tacaaaagaa gaacgggcaa aatacggaat
     1981 cactggtatg cttccaagta ctgtccaaac attggaacaa caatctgtcc aagcttacgg
     2041 tcaatactta agcaaacaga ccgatttgga aaaacgtatc ttcttaatga atctattcaa
     2101 tacgaaccgg acattgtttt ataaattgat gggcgaacat ttagttgaat tcatgcctgt
     2161 cgtttatgat cctgtcgttg cggattcaat cgaacaatac aacgaaattt tcttgaaacc
     2221 acaagatgct gtatttcttt caatcgacga accagaaaat atcaaagcaa gtttgaaaaa
     2281 cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca gatgcagaag gaatcttagg
     2341 tatgggtgac tggggtgtga acggtgttga catcgctatc ggtaaattga tggtctacac
     2401 tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta tcaatcgacg ctggtactaa
     2461 taacgaagaa ttattgaata atccattata cttagggaat cgtcatgctc gtgtagaagg
     2521 cgaaacttac tacgaattca ttgatcaatt cgttcaatca gctactgaat tattcccaga
     2581 attgttatta cattgggaag attttggacg aggaaatgct gcaacgatct tagaaaaata
     2641 cgaagataaa atcactactt tcaatgatga tatccaaggc acaggaatcg ttgtactagc
     2701 tggtgtatta ggtggattga atatatcagg tgagcaattg aaagatcaaa cgatcttgac
     2761 atttggtgct ggtactgctg gtgtaggtat cgcaaacatc cttttagatg aaatgatccg
     2821 tcaaggtgta cctgaagaag aagcacgcca acatttctat caagtagata aacagggttt
     2881 attgtttgaa gatacaaaag gattgactcc tggtcaaatc ccattcgctc gtaaacgctc
     2941 agaattcact aatgctgatg aattgacaaa cttagaagca gttgtaaaag caatccatcc
     3001 aacgatcatg atcggtactt ctacccaacc tggagcattc actgaagaaa tcgtgaaaga
     3061 aatggcggct cataccccac gtccaatcat catgcctttg tctaatccaa caaaattggc
     3121 agaagcaaaa gcaaaagatt tgattgaatg gacggacgga aaagctttag ttggaacagg
     3181 tatccctgct gctgatgtgg aatataacgg tgtaacttac caaatcggtc aagcaaataa
     3241 tgcgttgatg tacccaggtt tagggcttgg tttgattgct tcaacagcta cacgagtaaa
     3301 tgctgaaatc atttctcaag ctagtcgagc attaggcgga atcgttgatg taacaaaacc
     3361 aggagcagct atcttgcctc cagtagcaaa aatcactgaa ttctctcaaa cgattgctga
     3421 aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt gaagaaatca cggatatcaa
     3481 agaagctgtt gaatctgcta aatgggttcc tgaatataaa tctttagaag attaagattg
     3541 ttatagtaaa agaacataca gaaactttgt atgacgtcta aaaagaaaaa agcgactgcg
     3601 gcaaaacagc agccagccaa ctactgctct tttgccgctt ctctttggct tttttcatac
     3661 gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa acaacaaccg caactgcagt
     3721 tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt accggcgtaa gtttgcccct
     3781 ttatttgatc atggtccttg tcctgatcat tgcaatggct ttaggaaaat taccgacgaa
     3841 tatgattggc ccaatcgctg ttttggtcat tttcggtaat ttatttcatt ttatcggaac
     3901 aaaaatccct atagtcaaaa gttatttagg tggtggctcc gtttttgcga tctttgcctc
     3961 tgccgcgatt gctacatttg ggatcttacc tgagtatgta gtaaaatcca cagaaaactt
     4021 tgtcagcaat atgggctttt tagactttta tattgctgcc ttgattactg gtagcatcct
     4081 tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt tttattcccg ttgcacttat
     4141 ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt atgcttatcg gaaatggttt
     4201 tgcaaattcc gttttatata tctcattgcc cgcaatggct ggtggtgttg gagccggagc
     4261 tgtaccttta tcaacgattt atgcaaatgt tttaggtacg gacgcttctt ctatcatttc
     4321 ccgcctgatt cctgcgacag caatgacaaa tgtcctagct atcattggtg cagcgttagt
     4381 tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat ggtaaattaa tggaaaaagg
     4441 cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc attccacaat taggtgtcgg
     4501 tttgatggta tcattgactt tcttcatctt aggacaaatc tgtaactatt ttgttcctaa
     4561 agtccatgct tacgcattca tgatcattat cgttgttatc tgtaaaatcg caaacctatt
     4621 accagaatat tacgaagatg cagctatcat gttcaacaac ttgattgtca aaaacttgac
     4681 agcagctgtt cttgccggta tcggtattgc gttattgaat ctaaatgtat tggcttctgc
     4741 tttgacatgg caatttgttg tcttatgttt gacaagtgtt atcgcgattt cagtagtttc
     4801 aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa tcaactatta ctgctggctt
     4861 atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc ttatctgcag ctaatcggat
     4921 ggaacttatt gcttttgctc aaatgggcaa tcggcttggt ggtgccatcg ttttgatcat
     4981 ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa atacctgatc atcaagtatc
     5041 ggcttgatga ccaggtattt tttgataaaa atttacagga ttttcaaaaa tcttttttgc
     5101 attttatcgg aatgataacc gatgcgttca taaaatcggt gtgcttctac tctgtattct
     5161 gcagaattaa gtcttattgc tgatattcct cgtcttttcg cttcttctac gccagtcatc
     5221 aaggcctttc cgattcctct tttttcagca tgtttagaca cagcaagtgc cataatattg
     5281 aacatcggat cgctgaaaat cgattcatag gactccgctt gtacatagcc agctgctttt
     5341 ttagaagtag gatcttcata gaccaagaaa aaattttttt ctggctcctt caacagtttc
     5401 ttcatttgtc tcaccgtaac ggttaatggt acatcatatc ctaattgttc cgcattgatt
     5461 tctcgtagtg cttctgcatc tcctaaacgc atttcccgca acatccaaat caccagccaa
     5521 tttttatttt tttaaatgaa tttttttatc acagtatact ccttttttca cttaaaaaaa
     5581 gacttttcag tgattttgac ttgcttgaat ccagatcctt ctaatgtttg caagatgacg
     5641 aaagcattgt aaagattgat caaaagaacc acgatgacaa tcgcttgaaa aataaaggtc
     5701 actttttttg gagacaaaac cctgctaaat cttgctcccc acaaacctcc aataacagca
     5761 gcggggataa cataaaacaa catagataaa tcaaagcgca taaagctact tgttaaagca
     5821 atcgttacca attttgccag ttgagaaaag aaaatcgtag atagagaata tagtgttgct
     5881 tcttttattg gcaatgcaaa catcagcatc aacaaagaaa cattgactgg tccaccacca
     5941 atacctaaaa aactagctaa aaagcccaga acccccccac atataaggta ccaagtccat
     6001 gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga agaacgcaaa aaccaaggtc
     6061 aataccgtca aaaaaatttg gatcagctgc acatgcttct cgttttcaaa taactgtaaa
     6121 aaaacttcga atacaatgtt gccgactact cctccaacaa cagctccact agatacccaa
     6181 ccgacaatct gccaattcag tgatttcccg cttgatactt gtcgcacagt cgaaacaatg
     6241 gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc ctgcaacaga atgggcgccg
     6301 ataagatcaa gaatcggttt gatcaacacg cctccaccca tcccggaaat agcgccaact
     6361 gtgttcgcca gaacgatcac cacaaaataa atcagaccaa tcatcttgta tttcctttct
     6421 cttgtttata tcttttagcg attgttactc ttttctttta ttatacagta aaagaaacaa
     6481 acgaggcttc gaaagctttt ctacttttta aggaaattta gtgctaaacg aaaaaaccgg
     6541 gctctacttt tcagagagtc cagcttttct tacaccttat cgattctttt gttctattct
     6601 tcttccgcca tgaatgtatt gaatacttct tcgatcatat cccattctgc gtctgtttcg
     6661 atttgttcta actcgccttc tgttccatct tcgttttcga tgtatgcata agcttgtaat
     6721 tctacatctt catcttctga aacaccagct ggatataaaa gaacatagtt acgtccaaat
     6781 tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg tttcatttcc ttggtcatcc
     6841 accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat ggttatgttc tgtcataaga
     6901 ttacctcttt tctttttatt attaaaataa ttgcttacct gagccgtcta aataattttg
     6961 taaaatcatc acagctgcta atttatcgat gacttttttt cgtttcgctc ttgaagtatt
     7021 tgcctgctct actagcatac gttctgcctg aacagtggtt agtcgttcat cttgataatc
     7081 aaccggcaag gagaatagtt ctttgatctt ctctccatat gacatggaag cttctgccct
     7141 tggtccgatg gaattgttca tattcttagg taatccaact acaaaacggt ctacttcgta
     7201 ctgcgccact aattccttca accgatcaaa accaaactct tcttctgcct cattgattcg
     7261 aatgatttct actccttgag ctgtccagcc aaagggatcg ctcacagcaa cgcctactgt
     7321 cttagaaccg acatccaagc ccattactct cataaattca ctccatgatt tgagaggtag
     7381 tctttgacaa ttacttccat gatttcatcg cgttcgtgac ggcgaatcaa atttcgtgca
     7441 tcacgataac gaggaatgta ggccgggtct ccagaaagca agtagccgac aatctgattg
     7501 attggattgt agcctttttc ttccaaagcc cgataaacaa tcgctaatgt ttcgctcact
     7561 tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt catctgtaaa acccatgatt
     7621 ctacacctct ttcccgcaag gtaattccag cttaccttct acacccttat tttactcgaa
     7681 gaaagggaaa actacaaaca aatattccaa acttctgaat aaattaagaa acacgatttg
     7741 tctgattttt ccttttttct gtggcttttt caaaagccct ctttccataa agtatacgct
     7801 atatttcttt gaaagtatgt ttttacacaa ttttttcata aagttcataa aaaattcgaa
     7861 ttgctgctac taatttttcg ttttcgttgc ttgccctgtc tcataattga tggtgtagcc
     7921 atcttgaaca ttgtagataa agacattgaa ttcaattttt ttgtcttcaa tgctttctgc
     7981 ttccaactgc acacctcttg ctaccagttc atttccctcg aaatgaggag tgacacgata
     8041 gcgtacatga tgatttgttt ccttgatata tgccgcaata tcattttcgt gggcaagcat
     8101 gtacggagca ttcaacgaac gtgtccctgt cattaagttt ttaatgttat tattttgacc
     8161 agtcaactgg aaaccaatca aatggctgcg gttgtataac cattgtccgt cacttaattt
     8221 cttttgtttc caacctgtcg gatcaatata caaaggttct ctcttttcag taggaaacaa
     8281 gtcttttccc aacatcgcat tggcttgtcc tactctgttc aatttgtcta tatttgaata
     8341 gctttcccat gtccctttgg aaagactaag atcctctttt gtaaactccg gctgattccc
     8401 attgatttct atttcttgat gttttccatc atattctggc acatccgcta cttgagaagc
     8461 ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag atatctgaat ctttttgtgt
     8521 ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg atccccacga tcgccttggt
     8581 tgtttttttg ctcatttctt tactccttta ctaacagttc cctaaatctt aacatatgtt
     8641 cccctataaa taaatactgg aaagaaatag aaacagtaaa aaaataaaac cagtttttag
     8701 acggcttaag aatacaaaaa aagagacgga ttcatcgtct ctactcaatc acaaaacact
     8761 atttacaagg tatttattat cattcaaaaa atttttctta atactcaaaa ctaaacggct
     8821 tctgtacgac ctcttggtaa acagtgttca aaagcttgca tctgcggtaa taagtccaac
     8881 caagtcaaaa acataagaag cttttttgct tgactattct tattacttga tgcagggcag
     8941 ctttttcaca gcctctttta agcgggtgac gggaatcgaa cccgcgacat cagcttggga
     9001 agctgggatt ttaccactaa actacacccg cgatacaaaa acagtatacg cctctttcac
     9061 tccttcctca agttttttta ttctttacgc ttccgatatg ttttgtagta aaataaaact
     9121 aatcaatcaa ttattggaga agatgctatg acgggaaaag aattagatct gttgctggac
     9181 acttgtttac tcgctgggaa aatcatgatg gaaagcaatg cggagatgta tcgggtagaa
     9241 gatacgatga gtcgaattgc tttagcttct ggaaatttcc gtttggtcag ctatgtcact
     9301 caaaccggtc tctttattgg tcttgatcgt acctcgacca tccgaatgga acaaattacc
     9361 aaccgttcga ttaatttaga aaaagtagta aaagtaaatg atctttcacg taaatatgtt
     9421 gcttcagagc taacattaga agaactttac tgcgaactgc aagcaatcga ccgagaccgt
     9481 tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag tgatcagtgg aacaattatg
     9541 gtgttatttg gcggaaagat gaccgattta cctgtgactt tagtcattgg aggattggga
     9601 tacgcagctt atctatatag tttgaaattt tttagaatca aatttttatc ggaattttta
     9661 tcttctttat tgattggcat agccgctatc tacagcgtac gttttggctt aggaagcaat
     9721 caggatctaa tcattatcgg atgtgtgatg cctttagttc ctggagttca aattacgaac
     9781 gctatccgtg atttgctagc tggacattat gtctcaggag tttctcgcgg aacagaagcg
     9841 atgatgactg ctacgatgat cggttttgcg attgctttta ttttccagct tttttattag
     9901 gaaaggaaca caagtatggg gacgatattt attcagtttt cttttagttt tttagcgact
     9961 gcggcatttg ccgttattac aaatgtacct aggcgttcat tgatttgttg tggtctttct
    10021 ggtgcttttg gctggatgat ctactggatc tgtgttcaaa taggcggatc tgctgctttt
    10081 ggttcattgc ttggttcttt aggagtagca ttcatcagcg atcttttctc taaacgtttg
    10141 aaaatgcccg taacgatttt caatattccc gggatggttc ctctagtccc tggtggttta
    10201 gcttatcaag cggttcgaaa tctcgttact gaatcctatc gagaagcggc tcattatgcg
    10261 gtccaagcaa ttatgattgc tggagcaatc gctttaggtc ttgtattatc agaagtcttt
    10321 aaccataaca tccgtaattt caaagtaaag cgtgacgcaa tcatgatgaa acgaaaaaaa
    10381 cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg tgaaagagac gttcagcctt
    10441 gagcgatgac aaaatatctc acaaacattg cgacaagtaa ccgcaggagc aataagaagt
    10501 aattatgagt attaaaaaaa gttttgtaaa aatagcttct cacattttta cataaaattg
    10561 acttaatact gagaaactga cttttgaaca ccctctatct gtaaaaaagg ggctgcgaca
    10621 tgatttttgt cacggcccct cttctttatt caaatttttt ccaaaacgtt cgttgataat
    10681 aaacgatcca tagagcggaa agtattagaa ctaaaccgat cacaggaata ttcactaacc
    10741 aaaatggata aaacaaagcc aacattccgt ataagacgat aagaataata ctgatgatca
    10801 atgaacccat catagttgcg actaaaccca aacttcctga acctctgtta aataattggt
    10861 taatattggt ccagttcagc agcagcaacc gataatctct agcgaaataa cgtagtgaca
    10921 ataagtagca gccgaataaa gaaccaaata gcaagctagg gataaaaaac aaaggcagct
    10981 gaaaagacag tccgcttaat aatgcgatcc caccagttaa aaaactctgc aatatccatc
    11041 cgaatctgaa cttttctttt aaatattggt tcatagagat tggcaaagaa cggataaaca
    11101 gaaaattttc ttgatccaaa gaaataagat tgctgataaa agaagtctga ttaactgtta
    11161 agaaagccaa tgcgatccct accaggaaaa caacaccgat taacttatta tctaaatcag
    11221 ttaattttat ttcgcctcct agggcaaaag caagaataaa aatgattgga gttaacaagg
    11281 aagaagaaag aacttgcata atcaaattag gttcttttac taattgagca ttgtaactaa
    11341 ataaaagctg atgcacattt tggttctttt tatgggttct ttttattttg aactcactgt
    11401 ttgaatcagt cagttgctct gtaagctttg gtactatcag ctgttttaca gcaaggaaaa
    11461 atcccagagc cgtgatagct agtattcccc aacccaataa accttgttga gaaagtggct
    11521 gactggcagc atcatagaat ggcaataata tcgaaatcgg atgccgatct gttaactcat
    11581 tttcaatagt ccccgtttga tgattcatcc acatgattcc agcaaatgcg atcagcatcg
    11641 agctgccaag caagatacta gaaactgttt ttctatgttt tttaaacact gctgttcgag
    11701 ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat aatcaaaaat agagaaaaca
    11761 aaaggattga gataatgata gtaagcaaaa tccctagatc agctcgccat ccggttaaaa
    11821 taaacatgac tagaagcgga aaaacaaaag gaatgatggt caaagcaact accaaaatct
    11881 ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa aaatacttgc aggtcatttc
    11941 cttcaaaaaa gatattataa atgactgata tactttgagt taaacctaac ataccaaaaa
    12001 gtgccacgta ataagtaaag aaacctggca atttaacaaa gttcatagca aacatagcaa
    12061 atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt ttgagacaaa agatagatag
    12121 ttaatcgctt tccctttttt cctttctctc ttgctttatt cgtgagctgt ggattaacat
    12181 ataaaagatt aactcgaatt aattctaata attgtcgctt atacatacgg tccatctcct
    12241 gtcgcttgcc ttccagccat tttcaaataa atcttttcta gcgtttcatc aggtgagcca
    12301 ttcaataatt cactaactgc cccctgataa atcaattcac ctttcttcaa aatagccagt
    12361 ttatcgcata actgttgtgc ggtatccaat acatgtgtag aaaaaatgac tgtttttcct
    12421 tttgcggcat gagccttcat catttctttc aaatcaaatg ctgcttgagg atcaagtcct
    12481 tgcaaaggtt catccaatac ccaaatatct ggatctggca gcaacgtgcc gatcaaaatc
    12541 gttttttgac gcattccatg tgaaaaactt gcgattgttt cattttgatg actatacata
    12601 tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt tttgtgtgtc taattcatag
    12661 gcagctgaaa tcaagtccca atattctcca gccgtcaatt gtaaaaaaat atctggggaa
    12721 tcaggcacat aaccgatttt ttgtttgata ctcaatcgat tttccgtcaa tttcattcca
    12781 tctactaaaa tcgttcccga tgatggttca ataatactaa ctaaactttt aatcgtagtt
    12841 gattttccgg caccattatg tcctaaaaat ccaaaaattt ccccttgtct tatcgagaga
    12901 gacaagtctt ttaatgcttg ttttttcccg taacttttac ttacattgat gaattcaatc
    12961 atttctgtac tccttttgtt tgttattact ttttattgta cccttcgtta ttggtggagt
    13021 aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg actaggctgc tacttgggtt
    13081 gttctcttct acaggaaaac actcttctaa caggtaagat atgagtcttt gttttcttta
    13141 aaatgattca ttttctatct tttcttgcat aaaattcttt agtttatcaa tacttctctt
    13201 tcccatctct agttgttctc catttgcaaa tgttactatt tcgtttactc tgctaataga
    13261 tgtaatgttt tcagta
//
LOCUS       DAEGIQ010000076        13235 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000076 DAEGIQ010000000
VERSION     DAEGIQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.077"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            31..330
                     /locus_tag="KMT45_001959"
     CDS             31..330
                     /locus_tag="KMT45_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005414.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(427..861)
                     /locus_tag="KMT45_001960"
     CDS             complement(427..861)
                     /locus_tag="KMT45_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7005415.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1093..1803)
                     /locus_tag="KMT45_001961"
     CDS             complement(1093..1803)
                     /locus_tag="KMT45_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBE7005416.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1793..2653)
                     /locus_tag="KMT45_001962"
     CDS             complement(1793..2653)
                     /locus_tag="KMT45_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBE7005417.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2658..3296)
                     /locus_tag="KMT45_001963"
     CDS             complement(2658..3296)
                     /locus_tag="KMT45_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBE7005418.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3311..3610)
                     /locus_tag="KMT45_001964"
     CDS             complement(3311..3610)
                     /locus_tag="KMT45_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7005419.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(3653..5137)
                     /locus_tag="KMT45_001965"
     CDS             complement(3653..5137)
                     /locus_tag="KMT45_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBE7005420.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5157..5618)
                     /locus_tag="KMT45_001966"
     CDS             complement(5157..5618)
                     /locus_tag="KMT45_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE7005421.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5636..6703)
                     /gene="ulaG"
                     /locus_tag="KMT45_001967"
     CDS             complement(5636..6703)
                     /gene="ulaG"
                     /locus_tag="KMT45_001967"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBE7005422.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6921..7691
                     /locus_tag="KMT45_001968"
     CDS             6921..7691
                     /locus_tag="KMT45_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE7005423.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7964..8791)
                     /locus_tag="KMT45_001969"
     CDS             complement(7964..8791)
                     /locus_tag="KMT45_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005424.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9358..10593
                     /locus_tag="KMT45_001970"
     CDS             9358..10593
                     /locus_tag="KMT45_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBE7005425.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11592..11852)
                     /locus_tag="KMT45_001971"
     CDS             complement(11592..11852)
                     /locus_tag="KMT45_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBE7005426.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11879..13180)
                     /locus_tag="KMT45_001972"
     CDS             complement(11879..13180)
                     /locus_tag="KMT45_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7005427.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4198 a         2756 c         2088 g         4193 t
ORIGIN      
        1 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
       61 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      121 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      181 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      241 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      301 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      361 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
      421 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
      481 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
      541 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
      601 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
      661 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
      721 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
      781 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
      841 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
      901 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
      961 cttaaaatct ctctttcctt ttagaatgca tcctttttga taaataaaaa ggttgtgata
     1021 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1081 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1141 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1201 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1261 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1321 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1381 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     1441 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     1501 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     1561 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     1621 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     1681 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     1741 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     1801 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     1861 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     1921 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     1981 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2041 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2101 aattgcctag gtctggatat acttgtagaa aaggggaatg aatctgttct ttgatttcta
     2161 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2221 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2281 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2341 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     2401 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     2461 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     2521 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     2581 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taatgctttt tcataaatcc
     2641 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     2701 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     2761 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     2821 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     2881 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     2941 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3001 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3061 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3121 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3181 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3241 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3301 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3361 ctttggctga catcacattt ttgataccga taactgtaac ttcttttttc tctgcctgag
     3421 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     3481 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     3541 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     3601 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     3661 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataaccc tcaggatttt
     3721 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     3781 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     3841 cgatataacc accatatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     3901 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     3961 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4021 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4081 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4141 ttcctggaac agctcctggt aaaattgtgt ttgagattcc ttggaaagat tgagttagtt
     4201 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4261 aagtcgtcat aatgtagaat aagaaactct gtccttcttg catgaattgc gcttgaatca
     4321 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4381 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcgattt
     4441 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     4501 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     4561 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     4621 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     4681 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     4741 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     4801 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     4861 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     4921 caaccaaaat cggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     4981 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5041 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5101 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5161 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5221 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5281 ggatcttttg ctgcaagtgt aaaaaacaaa ctagccgttt tctcctcatc ctccccttca
     5341 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     5401 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     5461 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     5521 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     5581 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     5641 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     5701 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     5761 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     5821 cttttgaatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     5881 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     5941 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6001 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6061 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6121 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6181 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6241 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6301 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6361 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     6421 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     6481 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     6541 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     6601 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     6661 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     6721 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatgg aaaccctttt
     6781 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     6841 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     6901 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     6961 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7021 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7081 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7141 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7201 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7261 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7321 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7381 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     7441 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     7501 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     7561 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     7621 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     7681 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     7741 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgtg
     7801 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     7861 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     7921 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     7981 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8041 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8101 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8161 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8221 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8281 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8341 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     8401 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     8461 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     8521 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     8581 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     8641 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     8701 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     8761 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     8821 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     8881 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     8941 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9001 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9061 gacgacagca cacctatcaa ttaatataat tattttattt tttatgttct catgtttatt
     9121 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9181 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9241 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9301 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9361 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
     9421 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
     9481 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
     9541 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
     9601 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
     9661 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
     9721 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
     9781 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
     9841 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
     9901 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
     9961 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10021 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10081 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10141 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10201 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10261 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10321 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10381 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    10441 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    10501 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    10561 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    10621 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    10681 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    10741 ttaaaataaa agatctgacg caatccaaat gagcataggt agcgtctgcc atttattctt
    10801 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    10861 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    10921 ctttgtagag atttgtgctg ctagtccctt caacaattac taaatcaaat gtaggattta
    10981 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11041 tgcatactcc tatataaact gccagcaatc cccacatgct gagtagatca tgtcagaatg
    11101 ttctatgtcg tcagcaacga taaactaaaa gtcacgttca ccatgactga ttgctattcc
    11161 cagcgcaatt atttctatac acgtttcatg atttgaataa acactctcat catacatatt
    11221 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11281 tgccttgatc actttggtag tagatcgact aattcttaat ccattgctga taaaattttc
    11341 atttttgcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    11401 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    11461 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    11521 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg tcacaaccac
    11581 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    11641 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    11701 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    11761 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    11821 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    11881 agacagtttg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    11941 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12001 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12061 aagttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12121 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12181 gagctccaaa aatcatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12241 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12301 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12361 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    12421 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    12481 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    12541 ttaaaggctc gaataacgat aataaggtag cgtttacatc aaagcccgcg acgcttcgaa
    12601 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    12661 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    12721 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    12781 aactcatagg taagacccag ccaagaggtg ccttttcagg agttaaaacg tttacaggaa
    12841 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    12901 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    12961 tcatcctaaa aggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13021 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13081 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13141 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13201 aaaatatgaa ccattcattg catgacaacg caaag
//
LOCUS       DAEGIQ010000077        13147 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000077 DAEGIQ010000000
VERSION     DAEGIQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.078"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(78..150)
                     /locus_tag="KMT45_001973"
     tRNA            complement(78..150)
                     /locus_tag="KMT45_001973"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="KMT45_001974"
     CDS             531..1100
                     /locus_tag="KMT45_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBE7005428.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="KMT45_001975"
     CDS             1087..1947
                     /locus_tag="KMT45_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBE7005429.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /locus_tag="KMT45_001976"
     CDS             2137..2841
                     /locus_tag="KMT45_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7005430.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="KMT45_001977"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="KMT45_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBE7005431.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /locus_tag="KMT45_001978"
     CDS             4678..5994
                     /locus_tag="KMT45_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005432.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="KMT45_001979"
     CDS             5995..6864
                     /locus_tag="KMT45_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBE7005433.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="KMT45_001980"
     CDS             6919..7728
                     /locus_tag="KMT45_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBE7005434.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="KMT45_001981"
     CDS             complement(7831..8460)
                     /locus_tag="KMT45_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBE7005435.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="KMT45_001982"
     CDS             8630..9922
                     /locus_tag="KMT45_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7005436.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="KMT45_001983"
     CDS             complement(9998..10660)
                     /locus_tag="KMT45_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBE7005437.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /gene="groES"
                     /locus_tag="KMT45_001984"
     CDS             10885..11169
                     /gene="groES"
                     /locus_tag="KMT45_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBE7005438.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="KMT45_001985"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="KMT45_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBE7005439.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13081 aattataatg aaaatagtta tttttaatag ttataatatt ttatctatta aaagtaaata
    13141 tccattc
//
LOCUS       DAEGIQ010000078        12774 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000078 DAEGIQ010000000
VERSION     DAEGIQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.079"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..2111
                     /gene="acm"
                     /locus_tag="KMT45_001986"
     CDS             <1..2111
                     /gene="acm"
                     /locus_tag="KMT45_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBE7005440.1"
                     /translation="SLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEK
                     KQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKN
                     LDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTG
                     DMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYR
                     GEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFEN
                     HSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTF
                     ELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELK
                     FSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDA
                     ETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNE
                     NKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPK
                     YRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTER
                     EKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVI
                     IGLLIVTICAGIFLQKYKKV"
     gene            complement(2383..2868)
                     /locus_tag="KMT45_001987"
     CDS             complement(2383..2868)
                     /locus_tag="KMT45_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBE7005441.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    2958..3303
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3348..3989
                     /locus_tag="KMT45_001988"
     CDS             3348..3989
                     /locus_tag="KMT45_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBE7005442.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4008..4643
                     /locus_tag="KMT45_001989"
     CDS             4008..4643
                     /locus_tag="KMT45_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBE7005443.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4661..5476
                     /locus_tag="KMT45_001990"
     CDS             4661..5476
                     /locus_tag="KMT45_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE7005444.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5795..7231
                     /locus_tag="KMT45_001991"
     CDS             5795..7231
                     /locus_tag="KMT45_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBE7005445.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7420..9867
                     /locus_tag="KMT45_001992"
     CDS             7420..9867
                     /locus_tag="KMT45_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBE7005446.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10097..11164
                     /gene="rlmN"
                     /locus_tag="KMT45_001993"
     CDS             10097..11164
                     /gene="rlmN"
                     /locus_tag="KMT45_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBE7005447.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11357..12460)
                     /locus_tag="KMT45_001994"
     CDS             complement(11357..12460)
                     /locus_tag="KMT45_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBE7005448.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4353 a         2158 c         2713 g         3550 t
ORIGIN      
        1 catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca
       61 cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct
      121 cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa
      181 gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata
      241 aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata
      301 ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa
      361 ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta
      421 ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag
      481 gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg
      541 gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag
      601 atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag
      661 aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa
      721 ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg
      781 tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag
      841 cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta
      901 aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca
      961 aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag
     1021 atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag
     1081 ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt
     1141 ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata
     1201 cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta
     1261 aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa
     1321 gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa
     1381 cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta
     1441 aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg
     1501 aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa
     1561 catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat
     1621 tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc
     1681 gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag
     1741 acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac
     1801 cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa aattttccag
     1861 agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc
     1921 atacaggaga atccagcgaa caaagaagca attatattag taagagagct gtacctaaac
     1981 agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg
     2041 ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata
     2101 aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa
     2161 attccgattt ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc
     2221 aataaatatg gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac
     2281 tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag
     2341 aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac
     2401 tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt
     2461 agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat
     2521 cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc
     2581 tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt
     2641 taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata
     2701 gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga
     2761 aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc
     2821 cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc
     2881 tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat
     2941 tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga
     3001 gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt
     3061 ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat
     3121 gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt
     3181 aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt
     3241 gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc
     3301 ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga
     3361 ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta
     3421 ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta
     3481 agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt
     3541 tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg
     3601 aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg
     3661 gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc
     3721 cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg
     3781 tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag
     3841 gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc
     3901 taggcgtggc aatcatctat cttacattga ctggtattct aacaatcgta atgaaacaag
     3961 ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg attgaattga
     4021 agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc
     4081 cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac
     4141 gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt
     4201 tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc
     4261 agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga
     4321 aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta
     4381 gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga
     4441 ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt
     4501 tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg
     4561 taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac
     4621 taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct
     4681 attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc
     4741 agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt
     4801 tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc
     4861 caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat
     4921 gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac
     4981 gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct
     5041 gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg
     5101 tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga
     5161 gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct
     5221 gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa
     5281 acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt
     5341 tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga
     5401 tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt
     5461 tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc
     5521 cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca
     5581 acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa
     5641 aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc gactttttca
     5701 caacctcctt tacatatttt tattttgttg tggaaggggt ttcgtgttat tctaaaagag
     5761 gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt
     5821 tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca
     5881 agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt
     5941 taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc
     6001 tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt
     6061 tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga
     6121 cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca
     6181 agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag atttcactca
     6241 ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa
     6301 tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac
     6361 agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat
     6421 caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc
     6481 aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct
     6541 gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag
     6601 gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga
     6661 atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa
     6721 tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga
     6781 agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa
     6841 ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg
     6901 ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga
     6961 agataaacaa atccatgacg actatcgaat caattactta caaagccacg tcaaagccat
     7021 caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga
     7081 tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgatcaaga
     7141 cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa
     7201 actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc
     7261 tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat
     7321 aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt
     7381 tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct
     7441 actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg
     7501 ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta
     7561 ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa
     7621 atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt
     7681 tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta
     7741 ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg
     7801 acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt
     7861 gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt
     7921 tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc
     7981 tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt
     8041 gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca
     8101 gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa
     8161 tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa
     8221 ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat
     8281 atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca
     8341 gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa
     8401 gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca
     8461 actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa
     8521 actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc
     8581 acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca
     8641 gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt
     8701 aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa
     8761 atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat
     8821 gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt
     8881 tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt
     8941 gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg
     9001 tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa
     9061 gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga
     9121 gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg
     9181 aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg
     9241 gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg
     9301 gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc
     9361 aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg
     9421 atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca
     9481 atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa
     9541 cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg
     9601 acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa
     9661 gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt
     9721 cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt
     9781 ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa
     9841 acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa
     9901 ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc
     9961 taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta
    10021 tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa
    10081 aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat
    10141 caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt
    10201 gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga
    10261 aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc
    10321 ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact
    10381 gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg
    10441 ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat
    10501 tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt
    10561 ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga
    10621 ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt
    10681 ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa
    10741 tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa
    10801 ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa
    10861 ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc
    10921 gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc
    10981 atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag
    11041 attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac
    11101 agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa
    11161 ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg
    11221 aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga
    11281 caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg
    11341 gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat
    11401 gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca
    11461 catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga
    11521 cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga
    11581 ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga
    11641 cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg
    11701 agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa
    11761 caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt
    11821 tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc
    11881 ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt
    11941 tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg
    12001 ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc
    12061 cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg
    12121 gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac
    12181 ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact
    12241 gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt
    12301 cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa
    12361 tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg
    12421 ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc
    12481 gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag
    12541 aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca
    12601 ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg
    12661 ttcctcttag tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa
    12721 aattaagatt attatatgaa aacaggtttt atttgtcaat tcataattgg tttt
//
LOCUS       DAEGIQ010000079        12563 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000079 DAEGIQ010000000
VERSION     DAEGIQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.080"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(43..1809)
                     /locus_tag="KMT45_001995"
     CDS             complement(43..1809)
                     /locus_tag="KMT45_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005449.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1769..3523)
                     /locus_tag="KMT45_001996"
     CDS             complement(1769..3523)
                     /locus_tag="KMT45_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005450.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3633..4202)
                     /locus_tag="KMT45_001997"
     CDS             complement(3633..4202)
                     /locus_tag="KMT45_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBE7005451.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4220..5635)
                     /locus_tag="KMT45_001998"
     CDS             complement(4220..5635)
                     /locus_tag="KMT45_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBE7005452.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5646..6314)
                     /locus_tag="KMT45_001999"
     CDS             complement(5646..6314)
                     /locus_tag="KMT45_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBE7005453.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6444..7028)
                     /locus_tag="KMT45_002000"
     CDS             complement(6444..7028)
                     /locus_tag="KMT45_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE7005454.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7359..7592)
                     /locus_tag="KMT45_002001"
     CDS             complement(7359..7592)
                     /locus_tag="KMT45_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBE7005455.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7607..8557)
                     /locus_tag="KMT45_002002"
     CDS             complement(7607..8557)
                     /locus_tag="KMT45_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBE7005456.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8554..9396)
                     /locus_tag="KMT45_002003"
     CDS             complement(8554..9396)
                     /locus_tag="KMT45_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBE7005457.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9398..10117)
                     /locus_tag="KMT45_002004"
     CDS             complement(9398..10117)
                     /locus_tag="KMT45_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBE7005458.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10809..10967
                     /locus_tag="KMT45_002005"
     CDS             10809..10967
                     /locus_tag="KMT45_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005459.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            11039..12265
                     /locus_tag="KMT45_002006"
     CDS             11039..12265
                     /locus_tag="KMT45_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE7005460.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
BASE COUNT         4340 a         2218 c         1701 g         4304 t
ORIGIN      
        1 actatatccc aaaaattgta ggttaattta aagcctattt catcatcgtg attgagcttc
       61 tttttcatag acgaatttct cagatagttt agttttcttt atgagatttt tgtatatttc
      121 tgaccttcga tacaactcat tatgagtacc ttgatcaact agttttcctt gatccatcac
      181 tacaatttta tttacctttt caatagactt caagcgatgc gatataatta gtactgtctt
      241 atcttgtatt aaacgattta aactttcctg aatacgtttt tcattttccg catctaaact
      301 ggcagtaatt tcatctaaaa ttaaaattgg cgcatttttc aaaaaagccc gtgctatgga
      361 taggcgttgt cgctctcctc cagaaagtga acttccgttt tctccgacca atgtatgata
      421 accgtcaggt agtttttcaa taaagtcttc gcagttagct aaacgagctg cttttttcac
      481 ttcctcatct gacgcttgac tatttccaat acgtatattt tccaaaatac ttgtattaaa
      541 caaagttacc tcttgaaaaa caatagcgat tttgctaaac aaagaagctg gagatacacg
      601 tttaatatca tacccatcaa ttaaaataca gccttcatca taatcataca gtctagacac
      661 taatttcaag atactagttt tcccggaacc actagcacct actaacgcag tcacttctcc
      721 ttgtttagcc gtaaaagaga tatgatctaa aattggggta ttgttatcat atgagaaaga
      781 gacatctcgc aactcaatgt caaaagattt cagaggacta tctgatccct cttggatact
      841 ggtttccttc atagctctaa ttcgctgtat tttaggagct aaataaaata tttcaagcac
      901 tgcttctttc atagaatcga aggaatcctt tatttttatt gcggctagta agtaaccaac
      961 gacgtagagt atagtcactt cacccgttaa cagtaagtga acacctacta gaatgactac
     1021 tgctaaacta acatatgaga aaatagagga taaggccatg actgaaaaag tacttaattc
     1081 tacctttaaa tgaatccttt cactctcttc catttttttg aaaagtctgt cttgaacttt
     1141 tttagataga ttaaagctat taatctcttg ctgcaattct atagtttctt gaaattcttc
     1201 cgagttttct ctcaaagtat cgtaatattt agtattggct ttcgtttggg atttctttga
     1261 taacaagatt aagacaaaac taaataacgt tggcaaaata acagctaatc ccattttgac
     1321 attaccaacc aaaagcatca ctgaaataaa agggaaaaac agagccatac cgccggcttt
     1381 aggtattgca tgactcatcg catgctcaat accttcaacg tcagacatga tagtttgaga
     1441 taaatctgat aaattatgtt ttgaaaaata ggataatggt aaatttgaca aatcgtctgc
     1501 aatttgcact cttaaatgcg cactttcttt ataagtacta ttatataagt tttcatactc
     1561 cctatctaac aaccaataca tgaaaagcaa agtaacaaac gatacggcaa aataaagcca
     1621 acggggcttt gtattttcta aaactaattc gtctatcagc atcattataa tgaatgctgg
     1681 taccatatta atgcagtata caaaaaaact agaaatagaa gctttagtta aagcttctgc
     1741 gccctgatca cttaaagcaa atttgttttt ataaaaactt ttcattgtca agcctccaat
     1801 catttgcatt ctcataatta gtgaccaatt gtttatacac ttgatgttta gccattaatt
     1861 cattatgatt tccacgttct actattttcc cttctgacaa gacaagaatt tcatctacag
     1921 atttaatgct ggataatcga tgagctatca taataactgt tttttctttt atcagatgtt
     1981 gaaaagcctt ttgtagttca tactcattat ctggatcaat agatgcactg gcttcatcca
     2041 taataacaat ctttgcatct tttaaaattg ctctggcaat agctatacgt tgtttttctc
     2101 ctcctgacag ataaactcct ttactaccaa ttaaggtatg ctctctttcg ggaaattttt
     2161 ctaaaatcga ttcgcaaccg gctaatctta gcgcgtcaaa tactttactt gctggcgctt
     2221 gaggatttgc tatagaaacg ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa
     2281 agacaaaagc aattgtgtta ataattgtgt cttttgaata gtgatttaaa ggcttaccac
     2341 caattttaat ctgtccctta tccacattgt agaaacctga aattagttta gctatcgtac
     2401 tctttccgct tcctgatttt cctactaatg cataaattct tttttgttct aaaactaagt
     2461 tgaaattttc taaaacacac tcttctttgt atgaaaagct gacattttca aatgcaatgt
     2521 cacaattttt aatctgtgtc tcgttcccat attttagttg gtcttgcttc atttctaaat
     2581 aaagggagtc tagtgtttcg actgcatatc ttgcttcaaa caagtgcata cttacataca
     2641 ttattctcat gaaagaaaca aaaataattc cacttaaaaa aaacaacatc aacaagtcaa
     2701 cagctaatat cttgggagtg gtaagagaag ttataaaaaa ggttatagga actatcaaaa
     2761 tgcaaactat gccaaaaaat agccattgaa aaagaacaaa aggtgttttc ccactcaaag
     2821 aatacttata tgcaaactta gcataatcgt taattgcttt attcaaggct ttaaatgatg
     2881 ttactttagc accaaatatc tttataactt gaattccgcg gatatattct accgtttcag
     2941 agcttaacac atctaacgat tcttgatatt tttgaataaa atttgtattc tctgtcattt
     3001 tctttaacaa gaatacacca attactaaca tggcgattag aaccaatcca attttaaagt
     3061 taatgaaaaa gcctaaggca actgataaaa caggtgttac tatggcttga gcactatctg
     3121 gaatcaagtg agcaattgcc atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa
     3181 tatatcccga agaatttaaa tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc
     3241 ccttttttct caaattagtc tctaagcgaa acccaaggat atgagaaaat aagccagaaa
     3301 aaatataaaa taaagctcct aaagttaacc aagcaacagt ttgaaccgca taactcttag
     3361 ctaaatattc atctcctaca ataattagag actgaagaaa tttgtaaata ctgtaatatc
     3421 catagactgt taatacgaca gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt
     3481 tcattttagg gacataacta aataattttt tgtatacttt cattttttcc tcccccgcat
     3541 tcaatttctt ctcttattcg tttacaactg ttaatgcttt ccgtaatagc gtaaattaga
     3601 gatgtacaaa cagataaaag gtaaactacg ttctattttt tgtatttttt taagactttt
     3661 cttccaatca atacaccaat catagataaa cttgctacaa ctaacactga aatgattccc
     3721 catataccta ccgcatagtg gatcattgca tccattgttg cagcagattg tcctttatct
     3781 atccaatctt gacgaaaacg atctgtaaag aaccatatag gaatccactg tccggcagca
     3841 ctgcctaatt gcattagagc ccaagacgca acttgaggaa ttgctcgatc atatccaaat
     3901 acagatagaa taatatcagc taagatcgcc atactaatcg aactgataaa ccaaggtaag
     3961 tacgccccgc ccattaacgt aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt
     4021 ccttttctag ggtagttata gctaatataa acaaatatga ttccagataa taatccaaaa
     4081 atcccggggg atatgctgtg accaaacgcc ccaccaatac tactaaccat tattgaaaaa
     4141 aagtaaatta ctagggaaat aactgtagct aaaccaataa aaatataatc ttttgttttc
     4201 atatatattc tcctcaattc tatttcgtta tgtcattaaa aatctgttct atcaaagttg
     4261 aatcttctac tctaagtttt atatcttttt ttattccacc attttttaaa taaataattc
     4321 tattgcatgt tttataaaga aattccggat catgagtagc tataactact gcaccctttt
     4381 cttttaagtc tttaataagg tcagcaatcc tttccatatt aatataatcc aacccacttg
     4441 taggttcatc aaatagaaat aaattagttt ttgacaatga agctatagaa accaataatc
     4501 tttgtttttc tccaccggat aaatcaaacg gtaattgatc tttcatttta taaagttcca
     4561 ttttttttaa aattttttct tgctgatctt tactaactat ttcattcccc agggctaact
     4621 cgctcctcac tgattctgtg aaaaattggt aatccatttc ttgcattact aaaaacggcg
     4681 atgacaaatt ttttttattt tcgattatct ttcctttggt agggcgaata atcttcatga
     4741 gtattcttag taaggttgtt ttccccacac cgtttttccc taccaaacca ataacatctc
     4801 caactttgac atcaaaattt atgtctttta gaatattttc ataagtaatt tcttcaactt
     4861 ttaatacatc tgcacttacc ttttcgatat agggaacttc aatcgaacta ttaaaaatgt
     4921 caaatttcct taatgcgtta ttgttttcag tttttaacaa ctgaatagtt gtagttttta
     4981 cagttctatt tttaacaaat agaacttgat caattaattc tttcaaatag taaaaacgat
     5041 gttcagctat aacaatagta aatcctttat tttttaaatc acaaagtaag tcagcaagtt
     5101 tcttagtcgc tttgaagtcc aaatttgaag acggttcatc caatataatt aatttagagt
     5161 ttagcgataa actacatcca atagaaattt tctgccgttc accacttgat agcgaaaaaa
     5221 tgtttttatc taatatattt gaaagatcta ataattcagc taattcattg attcttttat
     5281 ccatatgctc ttttgtaaat ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat
     5341 ttgttgtaaa gaattgtcct ctaggatctt gacttacgta gccaatattt tttgatatac
     5401 tttcttgtgt taaggattgt agcgagttat tttcaaataa aatttcccca accaattttc
     5461 cgtcataaag tcttggaata agtccattca ttaactttaa tagtgtggat ttcccactcc
     5521 cagaagaacc agtaatcaat gtaagactgc ctttttttat tgaaaaatta aggtcaacaa
     5581 gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt gttaaattca aacaattctt
     5641 tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg aaaacaggat gatatagtct
     5701 aataagctca tgtttgtttt atagtaagaa cttcttttca caggagattc tatccccttt
     5761 gttattgcat tagcggtcat ttcttcagca atcatagttg cacgtataag aacgggaact
     5821 aacagaaatt ccaaatattg cagaggctga aaaaaagaca agcgaatgtt tctcattttt
     5881 aaagattctt taatcattct tatttcagaa cgataagtcg gaaagaatct gatcactact
     5941 gtaatactta acattatttt tttcggtaaa tgtatttttt ctaaagagga taataattca
     6001 ttgctagtat agacactaac caacaaccat gagatcatta cgaccggcaa cattcgaaat
     6061 acaatatata aggatgtcgc aaaaaatata gtaaaaaact ctatcttgat agcatcaata
     6121 attttcatta ctaaaaacag aactgaataa atcaagatga aataaataca tgtctttatt
     6181 ttacgaaaca tcaatactaa aactgtagca ataatgaaac accccacttg aaatatattt
     6241 ccctttccta aaaaaataat gaccggaaaa aaaatattta aagtaatcaa ggttaacgga
     6301 ttgatttttg acactctttc acctccaaaa aataagatcg cactgttatc ttaagctgat
     6361 aaaaatgcac cgttattcag ataacagtgc gatcttatca aaaagcataa ctgagaacaa
     6421 ttctcaatgc aagctttttt taattaactc agtaattcaa tccacccagg aacagaaaat
     6481 tgatacaatc ttctggttaa tagcatagtc ttttcttttg aatagctatg cttcaatata
     6541 tcatagaatg tagcgtagaa tgagtaaatg tagaagtgga tttcttcttc attaaagact
     6601 aaattttgat tgatcattcc gatagtcttt aacttatcta gacaacagac tgtctttttc
     6661 gtcacaaatg atgtaatttt ttcaacaata tcttgatatt tggttccagc tgattggaat
     6721 aaaatcaaat cgaatatacg tttattctca aaaatataat taataaatgg atctaattca
     6781 ttgaagttct ctctccaact tttctctata tctatatttt ctaagtttaa gaggtatttg
     6841 gtaaactctt cattatacat attaataagc gcattaatat gcggctgaat aatttcttca
     6901 aataacgctt ccttagaagt aaaatgttta taaaaagcac ccgtagttac gcctgcggtc
     6961 aggcacagtt gccttatctt agtttgctga aatccttctt tttcaaaatg gcttagtcca
     7021 ctttctatta acttttggtg agttataata taatctctgg tttctatggg tacttcctcc
     7081 taataagatg aattaacttt attatcttac acaggacttg aagtttcaat cgttaacgtt
     7141 tattactgaa ctcattcctt agagctaaac taatgagaaa gagcagaata actgatacaa
     7201 aaagcaatcc tacaagaatt aatagactca tctaaatcat tcaccttttc taaaataatc
     7261 cgagaaagat ttaaaaaaat tatatccagc tattaggata agtcctatca taatcactcc
     7321 aatagccatt ttcttatcct ccctaaaaat aaagtctttt acttaaaacc ttttttgtaa
     7381 tcgtcaataa attttttccc tttatcagtt aattgaaatc ctatatctaa atagtcctca
     7441 tatctaagtt tagccagcat ctcacttaca gatgctggac taacacttag aacttttgct
     7501 aaatcattat tttttaattt cttttttctt tttgataatt cctgaattgc tagtaaataa
     7561 attagtatag tagtcgtttt aatttgtacc atacctaccc tccaaattat ttcgaaaggc
     7621 cttcagcaat tttatccagg ttccatttca tcatcgcata gtaactatca ccatcttgtc
     7681 ctttttctgc aattgaatca gtaaaaatcg ttgaatagat aggaatattg gtatcttttg
     7741 atactgtttt cattggtcta tcatccacac tgctttctac gaataaggag ggaacttttg
     7801 ttgtgcgtaa tttttcaact aagtgtttta tttgatctgg tgttccttct tcttcagtgt
     7861 tgatttccca aatgtaagca gaaggcacat tatacgcttt cgagaaatat ttaaagcatc
     7921 cttcacttgt aacaatcatt tttttatcat tcggaattga agcaaattta gatttagctt
     7981 ctttgtctag agaatccaat ttttcaatat acttatctag attttcttta tagaattttt
     8041 tattatcagg atctttttcc gctaactgtt tttcaatatt tttagcgtaa ataataccgt
     8101 tttctaaatt taaccaagca tgaggatctt ccttcccttt ctcactctga ccctctaagt
     8161 aaataacatc tatgccatca cttgctgcaa aatagtcttt gttttcctct ttgttcgcat
     8221 ttttaactaa ttttgtaaac caagcatttc ctccagtttc caagttaaca ccgttataaa
     8281 aaatcaaatc tgcttttgaa gttttttgaa catcttcagg caaaggttca tattcgtggg
     8341 gatctttccc aataggtacg atactgtgta aatcaatttt atcttttgct atattttcgg
     8401 taatatctgc taaaatcgaa ttagtaacta ctacttttaa tttttcgttc gaagccactt
     8461 tatctttgtc tttactattt attgaacatg ccccgaatat aaaaagagca atcaaaatcc
     8521 ccagacttcc cagtactgct ttccattttc tcatcataac gatctctcct tctttcttaa
     8581 aaatttttgt tttggtgaga ttagaaaact aattacaaat aagaatgccg ctgttaaaac
     8641 aatacttgat cctgctgcta tgttaaaact ataaccaata aacaatccaa gcaatgatga
     8701 acttgctccg actgttgcag aaataaatat catagttttc aagctatttg catataaata
     8761 tgctgtagct gctggtgtaa ttaacattgc aacaattaat atagttccca cactttgcat
     8821 agctgttact gctactaaag ttaatagaac catcaataga taatgataaa aactgacatt
     8881 catccctatt gcttttgcca ttaatggatc aaaagaagtg attaataatt ctttaaaaaa
     8941 tagaactact ataaccaaca ctaaaaccga aacgccaatt gtaatccact tatcaatatc
     9001 ttgtacagct aaaatatttc caaataaaat atgaaataaa tccgttgagc tatttgcaac
     9061 tcctataagg ataactccta gtgctaaaaa agaactgaaa gtaataccaa ttgctgtatc
     9121 tccttttatt acactattgc tttttataaa agtaataatg atagaagcta atagaccaaa
     9181 tacaatcgca ccaataaaaa aattaatacc taaaatataa gaaattgcta ctcctggcaa
     9241 aacagcatga gaaatagcat cacccataag agacattcct cttaaaataa taaaacaacc
     9301 tactgttcca gaaacaatcc ctataactac agatgtaatt agagcattct gtaaaaagtg
     9361 aaagtcatac agaccattaa taaaattttg aagcatatta ttccccctct ccaataaata
     9421 tagtatctcc ataggctttt tttaaatttt cttcattaaa tacagattct acacttccgt
     9481 gagcaattag atttcgattc aaaagaataa tattatcaaa atacttcttt accttgctta
     9541 aatcatgatg cacaattagc actgtttttc cctgatgttt caattttttt aacgtatcca
     9601 taattattcg ttcactaact aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat
     9661 ccgcattttg agcaagacat cttgctaaaa gtacacgttg aaattgtcct ccagataatt
     9721 ctcctatttg atgattagaa tattttccca tatcgacctt ttccaaagcc tttgaaactc
     9781 tttgccattc tgcattttta attctttgaa aaactttcat tccggcatat gttcccatag
     9841 atacacattc ttttactttt attggaaaag taaaatcaat atcagctttt tgctctacat
     9901 atgcaatctt caccagtttt ttattgatat cttcattatt aatggagacc tctccctgat
     9961 gagggataat atttaacatt gctttaagca atgtggattt tccagcacca tttggaccaa
    10021 taatacctgt taaagcacct tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat
    10081 cgttataaca aacattaagc tttttcactt ctaacataac tttctccttc taaatgataa
    10141 tgattatcac gatcatatgg tatacaaaaa ttatatttta gtcaacccta aattttatag
    10201 tttagatttt tttctgttgc tgaagattag aatgacaaaa ctatttgaaa ctacgcgtat
    10261 agtaagtagc tttcaaaata tataaaaaag gagcgttctc aaatgagaac atgctcctaa
    10321 aactacttta ttggcgtatg gaaaaattat acttgaagtg gatagcgggt tgaataagtt
    10381 gataagcatc aaaacattta taaaaattat tcaagactat gtctgttcca tatacaaata
    10441 tgaaaatcag taaactccat ttaacttttt tcgtagtgtg tctttttata catgtttatc
    10501 gttattaata gtgaatagga aaaaaactat ttttgatcat tgacaattaa atacaatttt
    10561 tgaataacga tccctgctat tcgagctaga aactcaagta cgccatgacc tgattagcga
    10621 caaatactta gagatttctt tttgttctgg gaaaacaaaa tgccatgaca atagcagcaa
    10681 ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt
    10741 acccaaagca attcaaaaat ttcataacta ctattttagg agatgatttt atagaatcca
    10801 gagtctacct gaataaaaaa caaacctgtg catttctagg aatcgataat aatactctag
    10861 ataagtggat tcgtatatat aatttcccag taataagagt tggtggtgta gttcgctttg
    10921 acgtagaaga tattaaagac tttatgaata catttaaaat aacttgaaat tttcttctcg
    10981 ccgcatatac taaaattagt gaaatatact gccttacttt ttttaaggag tgaggatttt
    11041 gcctacaaag ctttcaaatg ggaaatataa aactaatctg agatatccaa aaaggtttaa
    11101 agaaataaca ggaattgcat cagaaaagta tcaaaaaaca tttccaaata ggcaattggc
    11161 gattaaagcc gaaaatgata tgaagaagaa aatagaaaaa gttttgagag aggagaatgc
    11221 aaactcatta gaattaaaag gaaaaataac gtttaaaaaa ttttacgaat ctaaatggct
    11281 tcctagatat gaattaggtc aaacaattag atcaaacagg cctcctagtg atataaccat
    11341 tagtaataca aaagatatat ttcggttgca tattttacct atgtttggtg aatacgcaat
    11401 gaattatcta aatatcaata ctgagataat atcagatgaa ttaaccaaaa aatcgaaaga
    11461 atatgcaaat ataaaaatca taaaaggata tgttcgttcc atgttcgata ttgcagaaat
    11521 actaaattat atcgaattta atcgaactac taaaattatt caaagtatca ctgcacctaa
    11581 aaaaaatgct ttagaagaaa aaagaataca agaaggtaag caagcactat catcaaaaga
    11641 acttactaat tggatagaag cagtaaatga tgattttaat aatcatttac ttacttttca
    11701 tgattacaca ctttttatga ttaccctgta tcttggtgat agaaaaagtg agacttacgc
    11761 tttgcaatgg aaatatattg attttgaaaa acaaacggtc cgtcttaaac atactttaga
    11821 taaatatcaa agaaaaaagt ttacaaaagg tcgaaaagat accgtcattc aagtaccaga
    11881 agttgtaatg acattgctaa gtgaatggaa atcggtacaa gctgatcaat tgttgaaatt
    11941 aaaaattaag caaacgttag atcagtatct ctttacttat actaaaccat ctggagaagt
    12001 aaattgtcct gttcatgccg attacctaaa ctatcgaatc aattcaatta aaagaaggca
    12061 tcctgactta gttcatttat ctccacacaa actccgtcat acttatgcaa ctatagctag
    12121 acaaggtgga gcagatatga atcaaatttc aaatgcttta acacactcag atatttctac
    12181 aacaaagata tatgtgaaca caccagatat agttgataag gctgtttttg aagcttttca
    12241 gagaggattg aataagtgtg attgatatgt gttcttaaat gtgttcttaa aaacaaaaga
    12301 agcactttcc aaataattga aaagtgccag aaacgttgat atataaagga tattaacgtt
    12361 ttgagaattg tgaagcttta cgtgctttct taagacctgg ttaaacaaac ttttcattta
    12421 taatgtttta cagagatata tgtcgtatga aattaatgtt ctcttgttca tgaagtttta
    12481 cagaattgta tagggttatc tgttgtcatt acctcaaatc attaccacaa cttggcgagt
    12541 tggctgattg aagaagtact ctg
//
LOCUS       DAEGIQ010000080        12228 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000080 DAEGIQ010000000
VERSION     DAEGIQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.081"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(190..354)
                     /locus_tag="KMT45_002007"
     CDS             complement(190..354)
                     /locus_tag="KMT45_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005461.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            649..1977
                     /locus_tag="KMT45_002008"
     CDS             649..1977
                     /locus_tag="KMT45_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBE7005462.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2179..3012
                     /locus_tag="KMT45_002009"
     CDS             2179..3012
                     /locus_tag="KMT45_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBE7005463.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3028..3765
                     /locus_tag="KMT45_002010"
     CDS             3028..3765
                     /locus_tag="KMT45_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBE7005464.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3920..4888)
                     /locus_tag="KMT45_002011"
     CDS             complement(3920..4888)
                     /locus_tag="KMT45_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBE7005465.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELKKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5120..5953
                     /locus_tag="KMT45_002012"
     CDS             5120..5953
                     /locus_tag="KMT45_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBE7005466.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5987..6826
                     /locus_tag="KMT45_002013"
     CDS             5987..6826
                     /locus_tag="KMT45_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBE7005467.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6852..7412
                     /locus_tag="KMT45_002014"
     CDS             6852..7412
                     /locus_tag="KMT45_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBE7005468.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7611..9332
                     /locus_tag="KMT45_002015"
     CDS             7611..9332
                     /locus_tag="KMT45_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBE7005469.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9497..10639
                     /locus_tag="KMT45_002016"
     CDS             9497..10639
                     /locus_tag="KMT45_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBE7005470.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10655..11866
                     /gene="thiI"
                     /locus_tag="KMT45_002017"
     CDS             10655..11866
                     /gene="thiI"
                     /locus_tag="KMT45_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBE7005471.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKETYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4162 a         2053 c         2542 g         3471 t
ORIGIN      
        1 tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactgccgg agtaaataaa
       61 gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta ttattatgat
      121 gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag tagagttggc
      181 tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca gcattcctac
      241 accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt tatctgtatt
      301 cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt tcattttatt
      361 catctccttt cttacgttat agtatttcct tctttcaatg agtattttga aagctatctt
      421 attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg ttatctcata
      481 gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc ttcatgaagt
      541 tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta tttctaaatt
      601 cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat gaaagctatt
      661 acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa acagcaggca
      721 ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt ttcagcacat
      781 gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc taatcaaatg
      841 caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa gatgctgaat
      901 caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg ggataaatat
      961 gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc aatcgatagc
     1021 cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg ggatatgatc
     1081 gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga aagtgcgaat
     1141 agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa agatgcagta
     1201 gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga aaagaaagaa
     1261 gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc tccaaaagaa
     1321 ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg actgacacca
     1381 caaatttttt atgataaata catcggtgta aaattagatg attatgttag cgtgatcaat
     1441 gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct aggaaatgtc
     1501 gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa aaaattagcg
     1561 attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg tcaatcttct
     1621 actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct atttgatacg
     1681 gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat gacccatgcg
     1741 atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa agtcgaaaat
     1801 agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc ctggatggac
     1861 gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca attaaatgcg
     1921 ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt agcatagacg
     1981 agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc cagctctttt
     2041 tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct aaataaagga
     2101 taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag aataaaattg
     2161 ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa attaaatggt
     2221 aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa aaccgaacat
     2281 cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga agcagaagta
     2341 gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat gctaggtgaa
     2401 acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg ggatcaacga
     2461 gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt tggttttcgt
     2521 aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga ttggtggact
     2581 tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac gaatcgatgg
     2641 aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa atcggttgaa
     2701 ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa tgtgttcttt
     2761 gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa tcgggaacaa
     2821 gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga tgcttatcaa
     2881 actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca gttatattat
     2941 cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga tcaattacta
     3001 gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa gcatgaagtt
     3061 tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt aggagtagtt
     3121 atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt cgtcaatagc
     3181 tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct ccctagagtc
     3241 ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt ctgttatcgg
     3301 aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc tgttttcgta
     3361 ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat tgctcctttt
     3421 gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat tgattcgggc
     3481 ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca tgttaataat
     3541 gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac tacgcatgtt
     3601 ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga gatcgaaagc
     3661 cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat ccgaatgcaa
     3721 gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag aggttgtgac
     3781 agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct caaatttttg
     3841 taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct ttttaagttt
     3901 agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca cccgacaaac
     3961 catagtttac agcaatcaat agttttaaac gtgctttttg actattaata ctgttacaga
     4021 aaataatccc catttttttt agttcttttc ccccgccttc ataatcataa acaggctctg
     4081 cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca agaagctttt
     4141 ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg attaccaaac
     4201 cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca gcatatgctt
     4261 taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt tctaaaagtt
     4321 cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat gtaggtgttc
     4381 ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa tggatctcat
     4441 cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg acacgaatcg
     4501 cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc attgccccag
     4561 tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca gtttcttcta
     4621 atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa gcggacaaga
     4681 tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga agattgaaga
     4741 tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct aataatgggt
     4801 ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg atcgttccgc
     4861 cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata attttgcata
     4921 gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa gaataacgaa
     4981 tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc aaccttgacc
     5041 tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc aaggaaatca
     5101 atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag aacatgacaa
     5161 attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac ccttagtgga
     5221 attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt ggataagtga
     5281 agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg gtcaaggaga
     5341 ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag atgttatgtc
     5401 gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct catcattcaa
     5461 atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg agggttatcc
     5521 tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg aacgtacaag
     5581 aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac agttgctgaa
     5641 tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt ttcgattcca
     5701 tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa aaagaacgtt
     5761 atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag tagttgattc
     5821 taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg cggctgaaaa
     5881 tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc caacccaaat
     5941 ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga tcaaagctat
     6001 tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg aatcaacaaa
     6061 acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa cggggcgcag
     6121 cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg tcgtgtataa
     6181 tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg aacaaaaagt
     6241 attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt ttggtgcgag
     6301 aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta tcaagcgact
     6361 agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc tccaggtttt
     6421 tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag aaccaattta
     6481 tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc gctttcccga
     6541 gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag gtggttcaaa
     6601 attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag aaattatggc
     6661 attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg gagtggccat
     6721 ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat caaatgaaga
     6781 agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa tgaaagaagg
     6841 aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc cacaaaactt
     6901 ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct catacacgtg
     6961 ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct aataaagaac
     7021 aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa gaaaaaatac
     7081 ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca ctgacagata
     7141 tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat cgaatattcg
     7201 aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct cattatgacc
     7261 ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct ccagaaccta
     7321 aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag aaatccgctg
     7381 atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt tgttatcttt
     7441 atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa atagtatgct
     7501 ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta tgccaagcag
     7561 aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa atgggaaata
     7621 atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc tttttggtta
     7681 gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa cgcaaggaag
     7741 aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg catctggttg
     7801 ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata tctacttcct
     7861 cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa agttttcggt
     7921 tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa atggagaaac
     7981 aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa cgcaactcac
     8041 tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc agagaagacg
     8101 agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg atcgaaaaag
     8161 aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc cgcgaggttt
     8221 tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa attccaccgc
     8281 tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt gatacttacg
     8341 agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa gatatcgcaa
     8401 aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa gttgaaacgg
     8461 tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat atcttagaac
     8521 gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca gagtatatca
     8581 aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca tctcaaagct
     8641 atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa gtggaagaaa
     8701 tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt ccttattcag
     8761 aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa aagcaacaag
     8821 tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg caagaaaaga
     8881 tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa caacgtttgc
     8941 ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt attgaagaac
     9001 taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt ttagttgctc
     9061 tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta gatgcagcag
     9121 cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac gaagaagtga
     9181 gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat caagaagcat
     9241 tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa cgcatcgaag
     9301 atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa ccagcaagag
     9361 caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt gaaacaagtg
     9421 aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa agaatttcaa
     9481 aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa tctttccgca
     9541 aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat caagcttgca
     9601 tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga tcgctgattt
     9661 gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag gagataactg
     9721 ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga tcatttcagg
     9781 agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag gaatcgaagt
     9841 aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga aagaattaat
     9901 aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag gatcgattca
     9961 gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt tccatgtaga
    10021 tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa gagttgattt
    10081 tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca tttattggaa
    10141 aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa atcagcgaag
    10201 tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt tatcattaga
    10261 gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa tcaatgcgtt
    10321 gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac cgcatatttt
    10381 atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag aagaaaaaca
    10441 aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa gcagtacatt
    10501 gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca gcttagatga
    10561 aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc tttataagaa
    10621 attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa ttatggtccg
    10681 ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc aactagcaca
    10741 aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg atcgggaccg
    10801 gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac tagcaaaaat
    10861 atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg aagttttgaa
    10921 acaagctgtt caagacatca tgaaagaaac ttatactggg aaagaaacat ttaaaattat
    10981 cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc aaacattagg
    11041 taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac cagatattcc
    11101 attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga tcaagggagc
    11161 tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg gggggattga
    11221 ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag ctgttcattt
    11281 tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc taacagccaa
    11341 actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg agattcaaga
    11401 agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc gaatgatgtt
    11461 gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga atggtgaatc
    11521 attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg aagtaacgaa
    11581 tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg aagtagcaga
    11641 acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta cgatttttgc
    11701 tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg aagagcgttt
    11761 agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag agatacaagc
    11821 agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg ttatgaaaat
    11881 agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg tattttaccg
    11941 tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa gttatggaaa
    12001 agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa gctacagttg
    12061 ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca gaagtgggca
    12121 gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt gaaatttcgg
    12181 cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttg
//
LOCUS       DAEGIQ010000081        12136 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000081 DAEGIQ010000000
VERSION     DAEGIQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.082"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..610
                     /locus_tag="KMT45_002018"
     CDS             <1..610
                     /locus_tag="KMT45_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE7005472.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="KMT45_002019"
     CDS             723..1001
                     /locus_tag="KMT45_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE7005473.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="KMT45_002020"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="KMT45_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBE7005474.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="KMT45_002021"
     CDS             1458..2213
                     /locus_tag="KMT45_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBE7005475.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="KMT45_002022"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="KMT45_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBE7005476.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="KMT45_002023"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="KMT45_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBE7005477.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="KMT45_002024"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="KMT45_002024"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBE7005478.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="KMT45_002025"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="KMT45_002025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBE7005479.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="KMT45_002026"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="KMT45_002026"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBE7005480.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="KMT45_002027"
     CDS             6950..7492
                     /locus_tag="KMT45_002027"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBE7005481.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /locus_tag="KMT45_002028"
     CDS             7515..9071
                     /locus_tag="KMT45_002028"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBE7005482.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="KMT45_002029"
     CDS             9085..9987
                     /locus_tag="KMT45_002029"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBE7005483.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="KMT45_002030"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="KMT45_002030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBE7005484.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="KMT45_002031"
     CDS             11430..11852
                     /locus_tag="KMT45_002031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBE7005485.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2154 c         2678 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtgctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAEGIQ010000082        12092 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000082 DAEGIQ010000000
VERSION     DAEGIQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.083"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(31..588)
                     /gene="frr"
                     /locus_tag="KMT45_002032"
     CDS             complement(31..588)
                     /gene="frr"
                     /locus_tag="KMT45_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBE7005486.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(591..1313)
                     /locus_tag="KMT45_002033"
     CDS             complement(591..1313)
                     /locus_tag="KMT45_002033"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBE7005487.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1449..2330)
                     /locus_tag="KMT45_002034"
     CDS             complement(1449..2330)
                     /locus_tag="KMT45_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBE7005488.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2429..3211)
                     /gene="rpsB"
                     /locus_tag="KMT45_002035"
     CDS             complement(2429..3211)
                     /gene="rpsB"
                     /locus_tag="KMT45_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBE7005489.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3570..4049
                     /locus_tag="KMT45_002036"
     CDS             3570..4049
                     /locus_tag="KMT45_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBE7005490.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4266..5357
                     /gene="phnW"
                     /locus_tag="KMT45_002037"
     CDS             4266..5357
                     /gene="phnW"
                     /locus_tag="KMT45_002037"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBE7005491.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5350..6135
                     /locus_tag="KMT45_002038"
     CDS             5350..6135
                     /locus_tag="KMT45_002038"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBE7005492.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6481..8172)
                     /locus_tag="KMT45_002039"
     CDS             complement(6481..8172)
                     /locus_tag="KMT45_002039"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBE7005493.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8594..9541)
                     /gene="arcC"
                     /locus_tag="KMT45_002040"
     CDS             complement(8594..9541)
                     /gene="arcC"
                     /locus_tag="KMT45_002040"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBE7005494.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9656..10675)
                     /gene="argF"
                     /locus_tag="KMT45_002041"
     CDS             complement(9656..10675)
                     /gene="argF"
                     /locus_tag="KMT45_002041"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBE7005495.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10766..11995)
                     /gene="arcA"
                     /locus_tag="KMT45_002042"
     CDS             complement(10766..11995)
                     /gene="arcA"
                     /locus_tag="KMT45_002042"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBE7005496.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3302 a         2528 c         2181 g         4081 t
ORIGIN      
        1 atgatccgcc cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc
       61 agcagcgatc ttatctactt ctttaatgct gttatctgtc aatgtttgta cgtctttttc
      121 taatccgcgc aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc
      181 gatcgcatca cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac
      241 atctttagct aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac
      301 atttccatca ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc
      361 ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat
      421 tgatgccatt tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa
      481 aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc
      541 tgcttttttc attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc
      601 cttacagttg ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt
      661 aaattgaaga caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc
      721 atgacttgta atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca
      781 tttgcatcta ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg
      841 ataacatctg cgccgatttc tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac
      901 gggttgccgg taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct
      961 ttacggcgaa tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc
     1021 ggtacaccca cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac
     1081 atacccatgt aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc
     1141 cagatatttc cgccacccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg
     1201 atttcttgga tgatttcttt gattactggt ggtttgattc caaatcctgc atccccagct
     1261 aaagcttctc cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt
     1321 cctccaatat tttctattgc cattttacca tacttagatg atttcgacac tctaagactg
     1381 gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt
     1441 caacagaatt attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt
     1501 tcgatacctt cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca
     1561 acgtattttt caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt
     1621 tctgctttga atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg
     1681 ccttcgttca atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga
     1741 atttgtgatt cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt
     1801 gcaactgttt cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata
     1861 tgcaagtatc caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag
     1921 ctgatttttt ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta
     1981 tctgttttga ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct
     2041 gcaatttctt ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag
     2101 tttacttcaa cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct
     2161 gcaatgcggt catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact
     2221 gctttttcca tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg
     2281 ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc
     2341 tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca
     2401 gcctttgaaa cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa
     2461 catctacgat ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta
     2521 cttgatcttc accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt
     2581 taacggcacg aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc
     2641 agtttgtatc aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa
     2701 tacgttcttt acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga
     2761 taccgcctaa gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt
     2821 taggtagaac ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga
     2881 tacgtttttg gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga
     2941 agtattgtcc tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac
     3001 ctacgaataa tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat
     3061 ctactaattt aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga
     3121 tatatttctt catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt
     3181 ctagtaattg tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt
     3241 tcctcttctt atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat
     3301 aagaatgtgg atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg
     3361 tatcgcaaga gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa
     3421 agaattcaag aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat
     3481 aattttaggt ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca
     3541 tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta
     3601 aacaagtcat tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc
     3661 attatggcgt taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg
     3721 tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc
     3781 ctgagactag gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga
     3841 aagtcgaacg agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat
     3901 ttgcatctgt tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg
     3961 atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc
     4021 accatccaac tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt
     4081 gtttttttat tttcaatatt ttccttatta aataaacctt tacactcttt acaaaaatac
     4141 atcatttttt attcaatttt ttcaacacct tgattgaatc acaataatca tcatttatat
     4201 tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa
     4261 atcatatgta taaattatta actcctggac cattgacgac ttctgaaaca gtgaaaaatg
     4321 ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa
     4381 gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc
     4441 aaggtagcgg ctcctttggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata
     4501 ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc
     4561 tgaaactgaa ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga
     4621 ttacacagct gattgaagag aatccctcta tctccgcact agcaatggtc catagcgaga
     4681 ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa gaaaaagatc
     4741 ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgattcgt gtagccgaac
     4801 tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt
     4861 cttttgtact tgctaataaa gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt
     4921 cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca
     4981 cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag
     5041 gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa
     5101 tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt
     5161 ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa
     5221 acggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata
     5281 ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg
     5341 agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc
     5401 tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact
     5461 gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt
     5521 tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa
     5581 gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat
     5641 tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat
     5701 attggttcca cgactggatt tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa
     5761 aaagaagggt attcaccaga cttcatggca acttctgaag acgtcgaagg gtatggtcga
     5821 ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa
     5881 gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg
     5941 acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa
     6001 ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct
     6061 ggcgctgata tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac
     6121 aagaatccag aatgactttt atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa
     6181 gtaataagaa cagccaaaca aaaatagctc ctcatatttt tcgtttggtt aggcttatta
     6241 ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa aaagggagct gtgacacgac
     6301 ttttgtcaca gtccccttat ttacaaacaa acagtgttta agagacaacc tctccactaa
     6361 acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct
     6421 attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc
     6481 ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt
     6541 cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt
     6601 atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga
     6661 cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac
     6721 cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc
     6781 tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc
     6841 tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt
     6901 caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc
     6961 caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac
     7021 ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat
     7081 caaaccaaat ggaatatggc gcatatcgtc agaccagtca aagcccattt ccttcaagac
     7141 agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt
     7201 gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt
     7261 cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat
     7321 ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc
     7381 catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc
     7441 atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc
     7501 ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg
     7561 ttctgtttca gctacttcat ggaattggac atataaacga agcaattcat tgattggttc
     7621 tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg
     7681 tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat
     7741 gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc
     7801 aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatat tgctatcccc
     7861 ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt
     7921 catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat
     7981 tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga
     8041 aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgttc
     8101 gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc
     8161 tttattattc atctttattc ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct
     8221 ctagcgacgg catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc
     8281 aattgtccta aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac
     8341 agaattctcc aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt
     8401 cgctagccaa gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt
     8461 tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc
     8521 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8581 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8641 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8701 tagctgcctc tactttcgga agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt
     8761 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8821 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8881 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     8941 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9001 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9061 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9121 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9181 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9241 ccttcagtac ttcgcccatg gcattttgta accagtaacc gatagatcct tctgtcattg
     9301 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9361 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9421 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9481 ttgcatccgt agacaagatt gcattacccc ctaaagcgac aactactttt cgtttggcca
     9541 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9601 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9661 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9721 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9781 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9841 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9901 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
     9961 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10021 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10081 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10141 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10201 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10261 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10321 gttcctctac cattttttga ctgaatccac ggaattcgat tccatcaaac atacgaccta
    10381 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10441 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10501 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10561 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10621 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10681 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10741 agtaagaccg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10801 tgcagcgtgg accaccacgg cctcgtgaca attcacttga attgatttct aacactttga
    10861 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10921 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    10981 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11041 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11101 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11161 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgtttgga attttgattg
    11221 ctaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11281 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11341 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11401 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11461 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11521 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11581 cgtatagact gctagaacgt acttcaatat cttttgtacg aaccccagcc atgattttat
    11641 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11701 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11761 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11821 atgtctttgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11881 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccttttca
    11941 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12001 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12061 attttgtctc attcaaaatg agacattcct ca
//
LOCUS       DAEGIQ010000083        11815 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000083 DAEGIQ010000000
VERSION     DAEGIQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.085"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            405..860
                     /locus_tag="KMT45_002043"
     CDS             405..860
                     /locus_tag="KMT45_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBE7005497.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(940..1305)
                     /locus_tag="KMT45_002044"
     CDS             complement(940..1305)
                     /locus_tag="KMT45_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005498.1"
                     /translation="MGISCLIFGIALAVGFFIASGMKITRLNHFLHHRKIPEKVLAEA
                     KEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIFFVTVGTGVFLII
                     YGSLVRHSYRQFTQSAYYW"
     gene            complement(1428..1688)
                     /locus_tag="KMT45_002045"
     CDS             complement(1428..1688)
                     /locus_tag="KMT45_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005499.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKNIKLTRRKQTQLLFI"
     gene            complement(1689..2015)
                     /locus_tag="KMT45_002046"
     CDS             complement(1689..2015)
                     /locus_tag="KMT45_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBE7005500.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            complement(2163..2603)
                     /locus_tag="KMT45_002047"
     CDS             complement(2163..2603)
                     /locus_tag="KMT45_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBE7005501.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            complement(2684..4081)
                     /locus_tag="KMT45_002048"
     CDS             complement(2684..4081)
                     /locus_tag="KMT45_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBE7005502.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGKFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            4234..5505
                     /gene="celB"
                     /locus_tag="KMT45_002049"
     CDS             4234..5505
                     /gene="celB"
                     /locus_tag="KMT45_002049"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBE7005503.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVSFIWYPFLKVLDKQPDLDIA"
     gene            5632..6351
                     /locus_tag="KMT45_002050"
     CDS             5632..6351
                     /locus_tag="KMT45_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7005504.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            6729..7703
                     /locus_tag="KMT45_002051"
     CDS             6729..7703
                     /locus_tag="KMT45_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBE7005505.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            7756..8361
                     /locus_tag="KMT45_002052"
     CDS             7756..8361
                     /locus_tag="KMT45_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE7005506.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(8455..9207)
                     /locus_tag="KMT45_002053"
     CDS             complement(8455..9207)
                     /locus_tag="KMT45_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBE7005507.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(9221..9481)
                     /locus_tag="KMT45_002054"
     CDS             complement(9221..9481)
                     /locus_tag="KMT45_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005508.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            complement(9503..10864)
                     /locus_tag="KMT45_002055"
     CDS             complement(9503..10864)
                     /locus_tag="KMT45_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBE7005509.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            complement(10937..11236)
                     /locus_tag="KMT45_002056"
     CDS             complement(10937..11236)
                     /locus_tag="KMT45_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7005510.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVDPIYDSIKELVQ"
     gene            complement(11249..11707)
                     /locus_tag="KMT45_002057"
     CDS             complement(11249..11707)
                     /locus_tag="KMT45_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE7005511.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQRLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDSQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITNYI"
BASE COUNT         3573 a         2413 c         2032 g         3797 t
ORIGIN      
        1 taaaaatgtt ctgtaataaa ataaaacttt ataacaacta taacaaaacc aaatacaaaa
       61 cagaagttct ataagttata atcatagttg ttaggtgatt catctagtaa aaacaccaga
      121 tttcaaattt cacacaattt aaactagatc aatcatcaac cttcttcaaa ttgttagaac
      181 cctttcccga ggagctgcag cataagcaaa aaaacaaaca ggaagccaaa tggctaagac
      241 attttttcta ttagtctagt tgtttctcgt cgcaagttta agactttaga aagttatttc
      301 taatgatgtt tgggttgttt tgatcaatgc ttatgcacag ctccttacct attatttatc
      361 tcaacagatc taacaaatta atggactatt taaggagttt ttttatgctt acgatctatt
      421 cttctcgcac acatagttac catctgatgg caaactggct gaaagaccat caaatgcctt
      481 acagagaacg aattttcaca cctaaacagc ctttaacaag gaaagagata ttttatttat
      541 tgtcattaac agaaaacgga tttgacgatc tattagctaa acgctcgatt gattataaaa
      601 atttgaaaga ccaaatcgac caatgtacga cctcagagct tgtcgatctg atcgtctcgc
      661 attttacttt gttacaacgt cccatcatta ctgacggaaa gaaattatta gtcggattca
      721 atgaagatag catgcgtctt tttatccctg tacaatggcg cagaagtcaa ctgaaatctg
      781 tctataacga ttttacatgg gtaagcaatc tagaaaattc tacggattta gatgatgaat
      841 ttttaaatta ttatatataa aaaagactgt gacaaaagcg tgtcacagtc tttttttcta
      901 cgccggataa atagaatatt tttacaaatc tgaatacatt caccagtaat aagcgctttg
      961 agtgaattga cgataagaat gacggactag actgccataa atgatcaaga agacacccgt
     1021 tcctaccgta acaaaaaata tagaggcacc gatactacca tccatatacc acattaaaga
     1081 agccaaaagt gggaaaagtg aaaaaataca taatccaatt cctgcaatca gtgagaagcc
     1141 aaaacttcgc tgatactctt cttctttttc ttttgcttct gctaatactt tttcaggtat
     1201 tttccgatgg tgcaaaaagt gatttaaacg tgtgattttc ataccagaag caataaaaaa
     1261 accgacagct aatgctatac cgaaaatcag acaagaaata cccatgaaca catatcctgc
     1321 tgaacaaaag aaaagaaaac ttgcaaaaga taagctaatg aatagaacac ctgatgccac
     1381 ttggagactt gccgctctgt attctttcca gtagttttct gcttcgctta aataaataga
     1441 agctgtgttt gtttccgtct cgtcagcttg atgttttttt cgacatctag ttcttctaat
     1501 aattcatcga ttgaaccaaa tgtcgaaatt actgtaccga tggcctcctg ttcatttttt
     1561 ccttctccca tcaattcatg ataatggtct tccatatttg ccaacaaatc agttttcagc
     1621 ttatctatct cggtactttg aggtactccg ataaaaagac tgtctaaata attacggatt
     1681 gtatccattt aatttccctc caaaaattga tcgactacgt ctttcgtcgt cagccactct
     1741 tgcttttttt cttctaaaag attacgccct atttcagtga ttttataata tgtccgcttt
     1801 tttccatgag tcacttctcc aggatacgaa acaatcagtt gattcttttc taaacgggca
     1861 aaaacggagt aaagtgttgt ctctttgatc acataggaat catttgtcct ctcacgaatc
     1921 gttttagaaa tttcatatcc ataagaatcg tttttgttaa ggatcgataa aataatcgca
     1981 tcattgtatc cgcgtattcc atcacttgaa atcattttct ttcctccata ttactccatc
     2041 tgtcgttgta attagaatag catgattact tcatcaggta aagtaatttt ttacaaaaaa
     2101 ataaaaggaa cagaaagcaa aagaagcgtt atgctttttt gctttctgtt cccttaattt
     2161 atttatttta caaccatgac gttacatgat gcgtggttga ctacatagga agtagtggaa
     2221 cctactagtg cacgttctac cgcattcaga cctgtcgccc ccatgacaat cagatcgatt
     2281 ggttctttcg attcatggaa attagcaatc agtgtctttg gataaccgta aagaacatat
     2341 gtctcgatat ctgtcaggcc tttttcaatt gccaagtttc gatgttcttt catctggttt
     2401 tccgcaacct catccaattc ttcctgaagg cttgttgtaa caaatgcaaa atcaccaaag
     2461 taattgcctg ttacttcatt gacatacagg atgtgcaacg ttgcctgatt tcgtttggca
     2521 atcgtgattg cctcaagaaa tgctttttct gccttttcag aaccatcaat tgctactaaa
     2581 atattacgat aagtatctac cattgtaaac gcctccttca ctgttagtgt agcacgatta
     2641 cagtgttaga acaaacattt tgtgaacgtt ttatcaaaaa atatcaggca tcaaatccgt
     2701 ttttttcaga gaccgtttta aaccagcgac ctgatttttt gatcgtccgt ttaccgtctt
     2761 gtgccaaatc aacagcgata aagccatagc gattcttata agcatttgtc caagaccagt
     2821 tgtccataca tgtccacatg tggtaaccca cacaattggt tccttcttgg attgcttgat
     2881 ggacgtattt caaatgatcc gtaacaaact cgatccggta atcatcttca ataattcctt
     2941 cgctgttaac aaatcgttct tctccttcga cccccatccc attttcagaa atatagcatt
     3001 tgatattacc atagttttct cttgtattcg ttaaaatatc atagattcct ttttcgtaaa
     3061 tttcccatcc acgatatgga ttcatttttt tattgggcat gtcgtaatta tcaaaataat
     3121 catctggcat ggggctatgt tcgtgattga ttggagtttc ttttgctttg attcgacgag
     3181 gctgatagta attgattcct agcaggtcaa tcgtgttttc acggatgagt tctaggtctc
     3241 cttcatggat ctctggcatt atagccaatt ctttgacaat ttcgatcaac tcttgtggaa
     3301 actttccttt cactgcaggg tcaaggaatg atcggttgaa gaatgcgtca gcgatcttcg
     3361 ccgcttttac atcttctggg ttcgtttcat ctctcggata gcttggtgtc agattcaaga
     3421 tgattccgat ctcaccatca tgtccgcctt catggtaggc tttgattacc tttgcgcttg
     3481 ctagattctc atgatagcct acttggaccg cttctttcat attgatatga tttggatagt
     3541 ggaattgata caagtagcct ccttctacag gtacgattgg ttcattatga gtaaaccatt
     3601 ttttgaccag atctccaaac agttcaaagc acgctgctgc ataagaaaca taagcgtcga
     3661 ccgtttctct tgataaccat ccgccttttt cttgcagtgg tagtggcata tcgaaatggt
     3721 aaaggttcac aaaaggttca ataccattat ccagcaattc agtaaaataa tctcgataaa
     3781 aagaaacagc tttttgattg atttcacctg tcccttctgg aatcaaacga ctccattgga
     3841 tagatgtacg aaatgtgtta tggccagttt ctttcattaa ctgaacatct tctttgtatt
     3901 ttgtgtaaac ttgcgaagtt ttttcaggtc ctacttgatc gaagaatttt tcaggctcta
     3961 attcatacca atgatcccaa atattttctc ctttaccatc atcacttact cttccttctg
     4021 tttgcggtcc gctagcagca gaaccccacc aaaaattttc tgggaatgtg taggttttca
     4081 tttttttact tcctctcttt tttcttgata ccgtaatcat aacgaaccct aaaaaataaa
     4141 tcaatacttt ttatgaaaat gactagacat ttaaaatttt tggcgttata ctttgtatgt
     4201 aatcgattgc aaataaattt gaggtgaata aaaatgaacg gttttatgga caagctttct
     4261 gagaaaatca tgcccttagc taatttatta gggcaaaatc gttaccttac agtgctgcgg
     4321 gatgctttca tgctttcctt ccctttaacg atgttcggtt cgatcgttgt cgttatcaat
     4381 aacttgcctt ttttcagtga cgcaacaaag ggaactttat cgaatttatt cggtaatggg
     4441 caaaatgcaa caatgagcat catgtcggtt tttgttactt ttggtatcgg ctattattta
     4501 tcaaaatctt atgacgttga aggtattttc ggtggtgccg tgtcttttgc cagcttcttg
     4561 attcttacgc cttttgcaat gaaaaccgct ggcggtgaag atgtgactgg tgtgctttca
     4621 cttgatcgtc ttggcgcaaa aggaatgttc attggaatga tcgctgcttt cttggctgga
     4681 gaaatctact gcagaatcac gaaacgcggc tggcagatca aaatgcctga tggagttccg
     4741 cctgcagtaa caaaatcatt tgccgctttg atccctgctg tagtgacact aactattttc
     4801 ttagtaatca atgctgtgat gactggtatt ttcaatgcta acttacatga cgtcgtttat
     4861 gaagtgatcc aaaaaccatt aactggtttg ggaagtagtt tacctgcaac attgattgct
     4921 ttgttctttg tccaattctt atggttcttc ggacttcacg gacaaatcat cgtcaactct
     4981 gtaatggacc cgatttggaa tacactgatg cttgataact tagaagctta tcaacatgga
     5041 aaagaattac cacacatcat cacaaaacca ttcatggaaa ccttcactgt tggtattggt
     5101 ggctctggaa tgactttagc tgtcgtttta ttgatggcct ttgttcttaa aaagaaacag
     5161 tatcgtgatg ttggacgatt agctttagca cctggtattt tcaatgtaaa tgaaccagcg
     5221 atcttcggtc taccgatcgt attgaatgca acaatcctta ttccttgggt tgtggcacct
     5281 ctaatcgtaa cgacgttcaa ttatttggta atggctgctg gacttgtccc tgcaccaact
     5341 ggcgtctctg ttccatggac tgtgccaatc attgcaagcg gtgtcttagc aacaaattca
     5401 tggcttggtg gtatgctgca agtcatcgac tttgtcatcg tttcctttat ctggtaccca
     5461 ttcctgaaag tactagataa acaaccagat ttagatattg cttaatagat cattaagggg
     5521 ttgtgacaaa aacttgtcat gacctctttt ttcgaataaa caatgttcca gatgctactt
     5581 cgagttgtcg gaagcataat ctttcgttat actctagaag aggtgaataa cgtgccaaaa
     5641 tacgaagaaa tcgcaaatat tttaagagaa cgtatccgtt caaaagagta tcctcccgat
     5701 agttttttgc ctaatcaagt cgacttggta aaagaatttg gtgcgagccg gatgacaatc
     5761 aaaaaagcca tcaatatctt agcaatggaa ggacttgtct attcgcagcg aggatcagga
     5821 acgaaaatct tgaatcatcc atttatgcaa aaagacacat cgcccgctaa cgagtatgaa
     5881 ggtttgagtc tccaaatgaa acgcagacat cgttctttga agagtcaaat cattcatttt
     5941 gatgttgaat tccctaatca atatatccaa gaacgattga tgatcgatgc cgagcaacct
     6001 gtctacaata ttcaccggtt aaggatctta gatggcgagc catatgtttt agagcatacc
     6061 tttatgccag tccatcttgt gccgggttta aaaaaagaac atttgttagc ctctatctat
     6121 gatcatcttc atcaagaatt gaatgtccat tttgcagggg cttatcgtgc tatttctgca
     6181 gataaaagtg atgaatatga tcagcaatat ttgaactgtg cagaaaacga tcctgtactg
     6241 gaagttcagc aagtcgtcta tctcaaagat ggacagccga ttgaatattc ttccagccga
     6301 aaccgatttg atgttcggaa ctattctgtg ctggatgtgc gttcaactta accaaagagg
     6361 ttgtgaaaaa gccgtttacc aagagatcgt gacagtggta agcagtttca agtaacaaga
     6421 agagatcgtg aaatcaacat tctattctat cctaagcatt agctcaaaaa tgaaaaaata
     6481 actcagcata aaaagactta tgaccacccg taatgattct aaagttagct ttttaaagct
     6541 agtttttttg aaatcgatac agctagtcgt aagtcttttt attttattga tccgtccctc
     6601 ctcttttcca ttcttgattc cttcctgata ctttagaaga caaattaatt gcaaaataga
     6661 gatgcaccct ctatactatg tctaaatcaa ctacttactt tttgtaagtt aacaggagga
     6721 aatttatcat ggatacacaa atcagaggaa ttcaccacgt cacagcaatg acatctagcg
     6781 ctacaaaaat ctaccgcttt tttacagata ttttaggaat gcgcttaatc aaaaaaacag
     6841 ttaaccaaga tgacatcgaa acttaccatc tttactttac agacgatact ggtgcgccag
     6901 gaacagatat gactttcttt gattttccaa atcagccaaa aggaaaaaaa ggaacagata
     6961 cgatctctcg tgcttctttc cgtgtgccaa gtgacgcagc attagacttt tggttggaac
     7021 gttttgaaaa atatgacgtt actcattcag caatcaatga acgtttcggc aaaaaatata
     7081 tcgaatttga agatttcgat aatcagtatt atcaattgat ctctgacgaa aacaatcaag
     7141 gtgtagcagc tggcattcct tggaaaaaca gcaatgtacc gacagagttt gcgatcacag
     7201 gacttggacc tgtctttgtc cgtgtcagtc aattagagca tatccgcttg attcttgaaa
     7261 aaatcttagg attcaaagaa acagaccatt caggagattt ctacttattt gaaatcggtg
     7321 aagggggaaa tggtgcaagc atcattattg aacatcggac agacttgcca aaagcaatcg
     7381 aaggatttgg aaatatccat cacttagcat tgcgtgtagc agacaaagaa gcattggaat
     7441 actggattca agtaatcaat aagattgaat tgccaaattc tggtttcgtc gatcgtttct
     7501 atttccaatc tgagtacttc ctagccgctc atcacgtact gtttgaatta gcaactgatg
     7561 gtcctggatt ccttgaagat gaaccagcag aatcagcagg agaaaaacta tctcttcctc
     7621 cattcttaga agaacgtcgt gaagccattg aagcttatgt ccgtcctttc gatacaagtg
     7681 atgcaaataa aaaacgtgaa taatctcttc atgctacttc atttgcatct atagaaacag
     7741 aaaagaggga acaaaatgac ctacattttt gaaaaaggaa caagtggcaa gcatttgatc
     7801 ttacttcatg gcacaggtgg cgatgaacat tctttactgg atatcgctca ctttcttgca
     7861 ccaaacagta cattgctttc ttttcgtggc acggtccaag aagatgggat gaatcgtttt
     7921 ttcaagcgta atggattgaa tcaattcgac ctggacagtt tggaaaaaga aacagataaa
     7981 ctccatgaaa aaatcaaaga aatcagcgaa aaagaactga taccgttaag tgactggctg
     8041 cttgtcggct attcaaatgg cgcaaacatc gctgctcatt tattattgga gcgtgagacc
     8101 gagctaaata aagggttgtt tttccatccg atgtctctag gtgttcatca aagccgttcc
     8161 acattagagg ataaaaccgt ctggatttct tatggtgata acgatccaat cgtttcccct
     8221 gcttccttta aagaattggc cgatcaattc gaaacacgcc aagcaaatgt gactattcag
     8281 ctaactgtaa caggtcatca gttgacgatg gacgaaatcg agcaagcgag ggaatggaca
     8341 gaccacctct taactaaata aaagaaaaga actacgactt acataataca acctgaagca
     8401 gatatcgatc tggatcagat tttgtgaaag tcgtagtttt ttgtgtcaga taggttagtc
     8461 tccaccagct gcaataacag atttattata aatagccgtt ttatgtttca actcttccaa
     8521 tcccccgatt ttctcacctt ttgaataaaa accgtcagta atccctaatg cttgaaaaat
     8581 cgtctgcttg taggcaattt ctgcattcca ttcaactgta tcaaggattt gcagtgcttt
     8641 atggatagct tcgattcctg attctccacc tgtgttgatc aatacgccat gagagttgag
     8701 cagcaacata tgcggtaatt cgagatgctg tttttttaca tagtcgcttg tcaattcagc
     8761 aagttcttca gagcaattag gctcaaagtc catgacaggg atctctttta ctttagctgt
     8821 ggcttcagtc aaattaggca taggcaaccc agtagtcgcc cagaacattg cattaggcgc
     8881 atgagcatgt aggacagctt tgatagctgg attagtctca tacatcgctt catgcatatt
     8941 gatttcacga gttaaatgac cgactccgtc aacgattcta cgcgttcttg gttctactac
     9001 tagaacctgt gcaggagaaa tctctcctag ataggcttct gacatcatgg tcggcgtcat
     9061 gatgatgtag tctttccctt gatcatcagt ggttttaaag gaaaaattcc ctcctgcgac
     9121 atttgtgttt ttcctttcga aaatcactct tacgatacgt gccatgtcct gtcgttctct
     9181 ctcaaataac atacgtccat ctgacataat gacccctcca ttatcttttt ataaaacttt
     9241 tttctctcaa tatttctttg gtagcttctt ttattttcat tgattcttct attttccgtt
     9301 ttcttttttc atcgtactct ctgaataaaa cgtgatttcc atatttatag atcactagtg
     9361 cgaaaaaaat gaccaataga ttgatgatga tattctgagt aaagagtcca tatagaaata
     9421 gcagaagtgt accacaaaga gcgactgtcc attttatttt atctgtctct tctcttttca
     9481 ttactttccc accatttcaa tgctattttt gtagttcagc ataacgttca ctcggtactt
     9541 tcgcttttat catatattta taaagtagaa caaacagtcc tagaaaaatc acgcatccta
     9601 caatagcgag aatattccta ttaaatgcct ccgcaaataa aaccctgaac tccggtcctt
     9661 ctatggatga ccatgtgatc aactgtcctt tcttgattcc ctcgactgct ccgctttttt
     9721 ctgctaaatc actgagtatt ggcgcaaggt aagtggcccc gtacaaataa ataggcatcg
     9781 tgaggattcc caagatcagc atacgagcca agtttcctcc tgttaaaagt aatccgccga
     9841 ccgcaatcga atagttgata atccctgcta atggtaatac ttggtttcct ggcaaaataa
     9901 tggcataacc aataaacaca ggaacaagta aaatagcagt aacccaaatt tctgaacgac
     9961 cagctaatat cggccagtcc aatccgatat atacttcacg atccttaaac cgctttttca
    10021 taaattctga catagcgtct gctagaggag acaaagattg catgaacatt ttagagatca
    10081 taggaaacaa acttaatgct gtagctgttt ggatcgctag attaagagaa cctgaaacgc
    10141 catacgcagc agcaaagcca agccccaatc caataatgaa tcccattaca gagttttcag
    10201 agaagatccc aattttcttc ttcaatgcat tcgtatccgc tcgtttattt aaagcaggaa
    10261 ttttatccat gatcatattg aaaggcagca ataagacagc ggcagatgtc ataaaatgag
    10321 tcactgttac taatggaatc cctgtgagat cttgtatatg tttttgaaac atatcaccaa
    10381 gctttaattc caatataact tggaccattg cagtaagaaa gcctgcagcc aaactaccgg
    10441 aaacataata aactaaataa gcagttaaag ctttccccca aacattccac atatcaacat
    10501 ttaacgtctt cgtccaatta aaaagtaaca gtacaaaatt gaccccaatc gttactgcaa
    10561 aaaatataaa agcataactc cacgaccatg tgatgcttgc tgcacctgtc cacccaagat
    10621 caagtgcagg caaactgatt cccgtatttt tagccaatgc ttctgatgct ggacttactg
    10681 cgttagacat atagccaatg accatagaca ttccgctaaa agcaatccct aaagtcaaag
    10741 cagacatgaa tgcttttttt aatttcatcc ctgccaacaa tccaagtatc aaaataatca
    10801 ggggaacaaa aatggctgat cctaagttca aaacataatt gataatggtt tggaaaactt
    10861 ccataaataa accttctttc tttcgaaata tagtaaataa aactatgaac caactttttt
    10921 atccaacaat cctcctttac tgtactaact ctttgatact gtcataaata ggatctacac
    10981 ccattccagt taaaaatgga attcctggaa atacttcaat acccaaatct tttgcttgtt
    11041 caagtacttc ctcatcaggt tgtgcgacat acacatagat tccagcagtt agaagctcat
    11101 tttgaatcga tttgtaatca actgcttcta caggaaatgc gatgccgtca tcctctaaca
    11161 tactttggat cttactagcg attgtctgac tcgttgctac tccacttcca catgcgacaa
    11221 ttaaccgatt tttcattttt tcttcctcct aaatataatt tgtaataagt tcgtatatgt
    11281 tttgcggtgt ttcttgtttc ttataatttt ccacgaatgt ttcatccatg aataaattca
    11341 taaacgcttg aagtaatcct acttgttctt ttggttcttt gattcctaaa aaaaatagat
    11401 tttctacagt catttcctga ctatccccca tttgataaaa ataaattggg ttcttcagct
    11461 gcataacaaa aacaaatggc ttttcgacat attcacattc cgaatgtggc agcgcgatat
    11521 ttaaatactt agtaatcaat ccagtaggaa attctctttc tcttgcaatc agtccattca
    11581 aataattctc attgacgtac cccatttttt ctaatcgttg tgcagcgatt aaaaaagctt
    11641 cttctttatt ttttacttct accgctaatt gaatcaaact aggaacgaac atctctttat
    11701 atttcattcc aacctcctcc tttttgatta agtttgttaa tttattgttc tactaagttc
    11761 atctttgttt tacaagaaca ttatatttct gttagtttgt tttgtcaacg ctttc
//
LOCUS       DAEGIQ010000084        11519 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000084 DAEGIQ010000000
VERSION     DAEGIQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.086"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(66..2141)
                     /locus_tag="KMT45_002058"
     CDS             complement(66..2141)
                     /locus_tag="KMT45_002058"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBE7005512.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAITDKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2143..3060)
                     /gene="glyQ"
                     /locus_tag="KMT45_002059"
     CDS             complement(2143..3060)
                     /gene="glyQ"
                     /locus_tag="KMT45_002059"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBE7005513.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3444..4256)
                     /gene="recO"
                     /locus_tag="KMT45_002060"
     CDS             complement(3444..4256)
                     /gene="recO"
                     /locus_tag="KMT45_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBE7005514.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4399..5298)
                     /gene="era"
                     /locus_tag="KMT45_002061"
     CDS             complement(4399..5298)
                     /gene="era"
                     /locus_tag="KMT45_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBE7005515.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5313..5708)
                     /locus_tag="KMT45_002062"
     CDS             complement(5313..5708)
                     /locus_tag="KMT45_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBE7005516.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5692..6168)
                     /gene="ybeY"
                     /locus_tag="KMT45_002063"
     CDS             complement(5692..6168)
                     /gene="ybeY"
                     /locus_tag="KMT45_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBE7005517.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6185..8377)
                     /locus_tag="KMT45_002064"
     CDS             complement(6185..8377)
                     /locus_tag="KMT45_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBE7005518.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8401..9372)
                     /locus_tag="KMT45_002065"
     CDS             complement(8401..9372)
                     /locus_tag="KMT45_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBE7005519.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10078..11457
                     /gene="brnQ"
                     /locus_tag="KMT45_002066"
     CDS             10078..11457
                     /gene="brnQ"
                     /locus_tag="KMT45_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBE7005520.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3107 a         2426 c         2118 g         3868 t
ORIGIN      
        1 taacaaataa accagagaaa cagcctattt tttgcctgtt ctctggttta tttacatatt
       61 agaaattatt ttgtgatgat ttgatccaaa cttgctagtg acaaagccat ggcagcaatt
      121 tgacttaatt gcgttaatcg gttcttacgt actgctggtt cttctgccat gaccatcgtc
      181 tgatcaaaat aattttcgat cagtgggcga aggcttacaa gctgatcata attttcgttg
      241 atcgtttggt cagcaaagct tgttttcatt gcctctactg ctttgtgcag ttctttctcc
      301 gcttcgtttt cgaacagttc agggtctact tctgattgtt tttcaagctc cgcttttttc
      361 gctaagttca cgacacgtgt caaagcttcc atcgatggtt tgaagtcttc atctgcaaga
      421 tgctgcttga acaattgtgc tgctgcaaat acttttgtca gatctttttg ttctgcgttc
      481 aaaacagctt cgatgacatc atgacggata ttcttcgttg tcaataattg gcgcagacgg
      541 cctttgataa agtcgatcac ctgttgttgt cccgctgaca agccaatgcc aaaacgatcc
      601 gtatctttac tgataacttc actaatcgtt tcttgcagaa cagacaaagg aaaggcccat
      661 tttttcgatt cgatgattcg aacgacacca tatgcttgac ggcgcaaagc atatggatcg
      721 tttgagccac ttgggatcat cccaactgca aagaaagaaa agatactatc taatttatca
      781 gtaatcgcta aaacagaacc tactgtagtc tcaggtagat ctccttcact ggatataggc
      841 atgtaatgtt cacggatcgc tgttgctacg gctggtttct cgcctttaag cagagcatat
      901 ttttctccca tgatcccttg tagttcaggg aattctccta ccatgttggt tactagatca
      961 aatttataga tttcagcagc gcgttgcaga tccatcaatt cttcctcaga tagtcctact
     1021 tgctcgccta tgatgcgggc gatgacttgt acccgttgca ttttctcata gatgctaccg
     1081 attttttcat ggaaagtaac agtttttaat ttttcaacgt attggtcgat tgaagctttc
     1141 ttgtcttcat tataaaagaa ttccgcatct tctaaacgag cagtcaatac tttttgattg
     1201 cctcgtacca cattttccaa ttttacccga tcgccattac gagcagaaat aaaatgagga
     1261 agcaacatac cttgatcgtt ccgtacttcg aaataacgtt gatgttcttt catagaagtg
     1321 actagtactt cttcaggaac agatagatat ttttcttcga agtcgccaga aaagacagtt
     1381 ggatattcga ctagattgtt gacttcttct agtaaatctt catctagatc aatgatccag
     1441 tgattttttt ctgctaattc gtttgcttgt gtgatgatca tttcttttct tttttgcggt
     1501 gaagcaataa caaattgttc attcaatttt tcttcgtatt cttttgcatg cgtaaatgtc
     1561 acatcttcgc ctaagaaacg atgacctctt gaaactctgt ctgttttgac atccaagatc
     1621 tcaaacggga ttatttcgtc atctaacaat gccacgatcc agtgaatagg gcgaatatat
     1681 tcaaaatcgt attctgccca gtgcatcgtt actgggaaag tgaggctttt aatgacatct
     1741 gctaaacctg tcaatacttc ttttgcttct ttgccgtgag tgtatttcgt tacatagaca
     1801 tattccacgc cttttaattc tctaaaagtg atagcatctg tcgtcagacc ttgtccgcgg
     1861 acaaatcctt cagctgcttt cgtccagttt ccatctgcgt ccaaagcaat ttttttggct
     1921 ggtcctttga cttcttcttc gctgtctgct tgacgttctg gaatgtttgt tactttgatt
     1981 gctaagcggc gcggtgtaga aaacgtttcg atcgtatcat aagtcaattg gttttcatct
     2041 aaaaattttg ctgttttttc ttctaattgt ttcatgcttg gtgtgactac atgagcaggc
     2101 atttcttcca gcccgatttc aagtaataag tcttttgcca tcttattcag cctccttcac
     2161 gttttctgga tttgctgtgt ttttattcaa taaagggaat cctaattttt cgcgttctgc
     2221 cacaaagatt ttggctacag cacgagccat gttgcggata cgggaaagat aacctgcacg
     2281 ctctgttacg gatactgcac cgcgagcatc taataaattg aatgtatggc tacatttcaa
     2341 aatataatca tacgctggat gaacaagatt ttcatcgata cagcgtttag cttctttttc
     2401 aaaacgatca aaattgctta gtagcaggtc ttggtcgctt acttcaaaag aatatttaga
     2461 atgttcatat tctggttgtt tgaagatctc accatatttc acgccatcag cccattccag
     2521 atcataaaca ctttcaactt cttggatata agaagcaaga cgttctagtc cataagtgat
     2581 ctcagaagta actggtttac aaggtagtcc tccgacttgc tggaagtacg tgaattgtgt
     2641 gatttccatc ccatcaagcc atacttccca gcccacaccg gcacaaccca tggatgggtt
     2701 ctcccagttg tcttcaacaa agcgaatatc gtgttctaat ggatcaattc ctaaaagacg
     2761 caagctttct aaataaagct cttggatatt ttcaggtgaa ggtttcatta cgacttgaaa
     2821 ttgatgatgc tgatacaaac ggtttgggtt ttctccataa cgcccgtcag ctggtcggcg
     2881 ggatggctct acataagcag cattccatgg ttccggtccg attgcccgta agaatgtata
     2941 tgggctcatc gtacctgctc ctttttctgt atcataagct tgcatcagca tgcagccgtt
     3001 tgatgaccaa aatttttgta atgttaaaat catatcttgg acggtcaatt ttttactcat
     3061 ctgttttgcc cctttcaatt tttaagcgtt gctgtcaata agaatacagg aaagaaaacg
     3121 acaaaaagtc cctatgtctg attacagaca tagggacgaa taatttcgcg gttccaccct
     3181 acttccgaat aaagattcgg cagcttcatt tatcgtactc gcaagtgcct cttcaaaaat
     3241 cgtttgggtc cggcttccac tctcccgaac tcgctaaacc acgtgttttt tactgatctt
     3301 ggtcaatgta cgtattcatt ttgcagtttt ctggtttgta taatagatcc tctcagtgaa
     3361 cgatctattt aatctacggt taataataga cggaagtccc tgtatttgtc aaatagtttt
     3421 gatttttttg ttgtatcttt catttattct tctttttcag gtatctcttt ttcaggcata
     3481 tttttctctg gcgtctttaa tacgtgtgcc caagacttca tttgatcaat gaactttttg
     3541 ctttttagat gaataccgac atattcatca tacaactggt caatgaggtc acggattgcc
     3601 tgcttcgttt cttctttcaa gtggattcca ctgatcttat cataagaaat agccgaaaac
     3661 attcgaacga aataaacagc gcgaggatct gcatgataac ggtgttcgtc cattggccag
     3721 tgtttctcgc atatcacacc gccatatttg gaagaatagt caaattttcc ttttgtttcc
     3781 ccgcatacga cacagtgcgt ccaaattggc gcgattccga agcgttgcag tatttgcact
     3841 tcaaagatat tcgtgatgat ttctgcatca tctccttggt ccatccgctg cagagcttgt
     3901 tgcgtaaatt ggaataaatg cgggtcatat tgatgatctt cgattgctgc atctactaga
     3961 tttagcaaat acgtaccata accgctcaaa aagatatctt cttggatgtt ccgaaacgca
     4021 tggacgtctt tgacactgct taaaaacgac aaaccttcac ttcgaaaatt accgatatag
     4081 actgcttcgg taaaggggag caaagccgga gctaatggct gattttgccg atggattcct
     4141 ttgatataaa acatcatttt gccagaagat tccgtaaaga ttttgactaa tttatctttt
     4201 tctttgtaat tacggctaga taaaatcaag cctttcgatt ctacaagctg gctcatattc
     4261 tccctcctaa aaaataaatc tggaaaagca gattctgccg atccagatta tttatgtttc
     4321 gatcttccgc tgtttttctc aacgtacgga tttttatcca gtttatttct atacctaacc
     4381 tataagaagt ccagaatatc agtactcgtc ttttcggtag ccgaagtctt gcagatagac
     4441 ttttttgtct cgccagtctt tttggacttt gacccaaagt tctagaaata ctttgtctcc
     4501 taaaagattt tcgatatcct ttcttgcttt cgttcctact tgcttcagca tttttccgcc
     4561 tttgccgata atgattcctt tttggctatc tctttcgacg atgatcgtgg cctggatatg
     4621 gattttatca ttctcgtttc gcttcatcga atcaacaacg actgcgacag aatgaggcac
     4681 ttcgtctcgt gttaaaaaca agactttctc acggatcaat tcagaaacga tgaagtattc
     4741 aggatgatca gtcacttgat catctggaaa atattgtggg ccctctggca tttcactgac
     4801 taatacatcc attaagcgtt cgacattgtt tccttctgta gcagagatcg gaatcacttg
     4861 tgcaaaaggc atctgcgtcg aataatcttc gatgattccc agcaactcgt ctggatggat
     4921 cttatcgatt ttattgataa tcagataaac aggagaatgc acgtttttta gacgttcaat
     4981 aataaaatcg tctccccggc cacgtttttg atcagcactg atcataaata agactgcatc
     5041 cacttctttc aacgcactgt aagctgcttc taccataaaa tctcctagac gatgctttgg
     5101 tttatggatt cctggtgtat cgataaaaat cagctgagct tctggtgtag tatatactcc
     5161 ttggatttta tttctagttg tctgtgcctt gtcactcata atggcaattt tttgacccac
     5221 gattcggttc aataatgtgg attttcctac attcgggcgg ccgacgattg cgacaaaccc
     5281 tgatttatgt tctggcatat acgttctcct ttttaattaa acaacaaatg atatatttta
     5341 gggacaaata aaatcaaccc aatgatgaca gcaaaacaag cggtcactaa aactgctcca
     5401 gctgccatat ctttgatttt tttcccaatc ggatgaaaat gaaaatccgt aaacatgtcc
     5461 actacatttt caaaaacagt attgatgatc tctactatcc aaacgagaaa aacagctaaa
     5521 agcagccata accactctat ccgatcaagc tggaaaatca cgcccataaa taaagcaagg
     5581 ataccgaacg caacatgttt cctcatattg cgttcttcct caaaaacggt tttgactcct
     5641 tggacagcaa attccacaga catcaggaaa tgtttgtttt tctctacgtt tttatctttt
     5701 gagtccatag gcatccaaga tctctttctg taaaccaaac atttctttct catcttctgg
     5761 tgtcatatgg tcatacccgt tgatatgcaa aaatccatgt accgctaaaa agccaagttc
     5821 ccgatcatat gaatggccgt aagcttctgc ttgttcagca gcacgttcaa tagagatcat
     5881 gatgtctcct agtacacgag gtaatggatt ctctccatct tcaaaaatca ctgggatttc
     5941 gtcttcgcct tcttcttcca gagcaaaact gatgacgtct gtcggtgcat cttttccacg
     6001 gtaatcacga ttgattactt gaattgccgc attgtccatg aaagtaacag acatttctgt
     6061 ttcctctggc agctttaaat aagatgctgc aaattgcagc aagtcatcga tttctttcat
     6121 tttctcttct gagacttggt ttgtttcatc cataaaggta atatccatta tacatcgctt
     6181 ctttctattc ttcttgtact tcctttatct tctcaacttc tgttttcatt ttaggatatt
     6241 tgatccgaga atggaaggtg ctacttaaac cgctgattaa tgatttttcc acttttcgga
     6301 tttctttcaa ggtcagtcca ctatcgtcta attgaccatc ggaaatacgc tcttcgataa
     6361 gattatggac aaaactttcg attttttcgc tcgtcggatg atccattgcc cgtactgcag
     6421 cctcacaact gtcagcaata ttgacgatcc ctgcttcacg actttgcggt tttggtccag
     6481 gataacggaa ttgctcttct gttacttccg gattgcgttc tttcgctttg atgtagaaat
     6541 aacgcattag cgtggttcca tgatgctgtt tgcaaatatc gatcaccatt tgcggcatat
     6601 tataatcttc caatattttg gctccatctg tcacatgtcc aaagataata tgtttactgt
     6661 cttctggcaa taagaaattg tgcgggtttt ccgctcctga aggcaagttt tcaacgaaaa
     6721 agctagcatg tttgatcttg ccgatatcat gataatagca agctactcga gttagcaaag
     6781 aacgtccgcc gatttctgct acagcgttag cacttagatt agctaccatc atactatgat
     6841 gataagtacc tggcgcttct tccagcaact gtttcaataa aggatgattt ggattgctga
     6901 gttcatttaa tacaattaca ctgtcatcat tgaccatcaa ttcaatatat gggtgtagac
     6961 cgatcgtcag caagaatgag aagagtgcac cagtgaaccc gcagacgagc attaaccaag
     7021 tcgttgcatc accaaagctc attccttgat agacgatcaa aatgacatcc atcatcacag
     7081 gaaaaataat gacccacatg gcagcagata atccttgttc tgagatccgt ttgcgtctga
     7141 caatcgttgc catgagccca gagaaaacat aagacatcaa gataatcgtt agaaagttcg
     7201 tgccaatcga atcataaaaa atgaaaagtg cggatactgc ttggaaaata ccagccatga
     7261 tccctgctct tcgattcaag aaaaagctta aaacaagtgg tgcaaaagca gcgggataaa
     7321 acagcgggat ataagtagct tgttccgttt ggaataattg gaaaaatttc atcagcacta
     7381 tgctgactga catagctgtc acatagaaaa gcacgtattt tgttctttct cctgcatttt
     7441 ggaattgaag tgaattataa atcagtacgc cgatttgaag caaaacagct aaaaccatgg
     7501 caattagcgg aaaaatcgaa gttgtttgat tagtcagtcc tagtaattcc aattttttca
     7561 tagctgaagc atcgatttga ttaccttcac gaacaatgat ttctccttgg aagatcatca
     7621 ctggttgtac agcttctcgt gcggattgtt tcaattcttc tgtctttttc tcattcaata
     7681 cgtcattcac tacgatacct tgatccacta agtagcgaat catttgttgt tgtgctggtg
     7741 tgacattcaa atattggatt tgctcttctg cttcttgttt aaaagcttcc aattcacttt
     7801 cacgaacttg tttgctcatc tgttcgtcaa tgagcttcag actctcatcc ctcacagtat
     7861 gaatctcagt ttcagtcatt tcaaatatcg tcgaataaaa attgttcggt agtttttgat
     7921 aaaacacaac attttctgca ttcacatttt caaaattttt cttcagcgcg gcgatccgct
     7981 cttctgtagt aggctttggg atattttcat cttcctttgc cttatcaatc ttttcgtcat
     8041 aagatttgtt gatggcatca ttcgtcttat cgatcaattc aaatagttgc ttgatccgat
     8101 tgttctggtc atctgcaaga tctttttggt aagtgtattc tggagtgact gcttctgctg
     8161 ccagttttct tttttgttcg gtttcttccg tgttctcgat cgttttgttc gcacggatac
     8221 tttcttctgc tacttggcct tctctgaaat cattggcctt ttgtttgaca ctactaaatg
     8281 taacgccgca gataatgata aaaaacaaga acagcaggaa aggcaatgct gcctttccaa
     8341 gtttttcttg tagtttctgc agtgttttat tcaacatgtg cttatcctcc taagcaaagc
     8401 ttattcttga tggagatctg ccttttcata agctttgatg atttcggcaa ctacagggtg
     8461 ccgaacaacg tcacttgctt caaaattaac aaaatcgatt ttcttgattt gctttaatgt
     8521 tcgttctgca tgaacaagac cacttgtcgt ccctcgaggc aagtctatct gacttgtatc
     8581 cccattgaca atcatttttg attgataacc gagacgggtc aagaacattt tcatctgagc
     8641 gactgtcgtg ttttgtgctt cgtccaagat aacgaatgca tcatccaatg ttcgcccccg
     8701 catgtaagct aaaggtgcga tttcgattac gcctcgttcc atcaatcggt tcgtatgatc
     8761 caatccaaaa atttggtata aagcatcata aacaggccgc agataaggat ccactttttc
     8821 ttttagatcc ccgggaagaa agcctagatt ttcacctgct tccacagcag gccgcgtcaa
     8881 aatgattttt tgcacttcac cttttttcaa ggcagcgatt gctaatacta ctgcaagaaa
     8941 agtttttcct gtcccggctg gtcctacgcc aaatacgata tcatgtttgg ctaccgattc
     9001 gacatatttc ttttggccgc tgttttttac ccgtataggt tttccattac ggtctttgac
     9061 gatctcttcc tcatacatct caacaaaata atctaatgta cctttttgcc ccattttcaa
     9121 tgccgtcaca acatcgggcg tacttacgtg gattcctcgt ttgatcaatt cctgtaatgt
     9181 ccgaatgact gaggcagcca aggaaatctg ggattgctca ccaatgatct gaatgatttc
     9241 tccacgtgta tggatggttg tttccgttgt ttcctcaatc agttttatat gcttgtcgtg
     9301 tgcacctaaa agcatgctga catcatcagc agccgtcaat ctaatctcta aagaactaga
     9361 ctcttccgtc aaaaatagac atcccctttt tttcgattaa ttctttccta ttttaccata
     9421 ctcagaagaa actatatagc aaataagaaa aggttctaaa agagttgctt aaccataagt
     9481 accaagaaac gttaaaaaac tagccgcttc cattctctaa aggtctcgtt tgaataaaaa
     9541 aagctgatca tgtaagctat gcataagata gaggacaagt attccgtcta acttagcaag
     9601 tattctctgg gtatttttct tgtttcaaaa aaagaaaaca aaatatttga aatattcttg
     9661 catttcaaaa aaaacaggtg tattataacg tcaacaaata aatatacagc aaagcgtcga
     9721 ggaaaacagt aggctattca gtgatttatt cccagtgagt catgtaagtg agaaatgaca
     9781 ataaatcgaa tagacgaaca tcatttccga gtcaatgtcg aaaacgtaga tcattgtggc
     9841 tgcctccatt atcaggctag cggataactt tatcgaaaag cgtccgtgaa tgaagaagct
     9901 tttttaagcg aaaaaaggtg gtaccgttct agaatctaga gcccttttcc caagtggaaa
     9961 agggcttttt ttatatttta aggtggtggt tttcatggaa tctgatatta gtggtatttc
    10021 tagtttctgc aacgtttatt ttgttactca aaatgataat tattggagga aaaatttatg
    10081 gaagaaacaa aaacttattc atggaagcaa ttattgatgg tcagttcact tatcttcggg
    10141 atgttcttcg ggtcaggtaa tctgatcttt cctgtacact taggtcaaat ggctggatca
    10201 aactggatca gcgctggtgt aggatttgcc atctcaggcg ctcttttccc attattagct
    10261 atcttagctg tagtagtgac aaaaagtgat ggtctgtatg acttagctaa acctgtcgga
    10321 aaatggtatg ctgccttgtt cttgatcctt gttcatttaa cgatcggacc attctttggt
    10381 acaccgcgga cagcagcaac tgcttttgaa atggctgcta aacccttttt gcctgaaaaa
    10441 tatgattcat taggtatgtt tttattctct gctcttttct ttttacttgc ttatttcctt
    10501 actgtacatc caacacgttt ggtcaaatat gtcggaaagt atttaaactc cttgtttctt
    10561 gcattattag ctatcatttt tgtcgtttct ttattccaac ccatggggaa tctaaaccaa
    10621 tctgctgcag attcttatca gacgaacgct ttgacaaatg gcgttttaga aggatacaac
    10681 acggttgatg cagtagcttt acttgcttta agtgtcactt ttgtccatgc agttcgtgat
    10741 cttggtttta aagataaaaa agtcacagaa ttaacagcaa aagctggtag cctgagcgtt
    10801 gcgttagaag tcatgattta ttttggtctg gtccttctcg gtgcgatgag tttgaataag
    10861 atgtctttat cagaaaatgg cggaactgca ctttctcaga tcactaattt ttatttaggt
    10921 aacttcggtg ctagcttctt aggggtaatg gtcacattag gtgtcttcac tactgctatg
    10981 ggactcgttg tttcttttgc acaagatttc cataaactgt tccctaaagt cagttatatg
    11041 acatggctgc ggttaacgac ttttgtctct tttgtcgttg cgaatgccgg attggataat
    11101 atcatccaat ggtctttacc agtattgatg cttttgtatc cattgtcact tgcgttaatc
    11161 ttactttcct taacagcaaa attcttccaa aagagtccat ttgtctatca agtaacgatg
    11221 ttcttttctg ccgttccagc cgtattggat atgttggcaa attcgcctgc attagtgagt
    11281 cagcaaactg tcgtagcaag cttactagga ttttatcatc attatgttcc ttttgctgca
    11341 ttaggtcttg gctgggtcgt cccaacattg cttggttacg ctggcagctt gctcttttat
    11401 tatgtatatc gcgtatcagg atataaacaa gaagcaagtg agttaccaga agaataacca
    11461 aatcaaacac aatttaacat aagagatatt aaacaggaaa aaaacagcta gaagaaaga
//
LOCUS       DAEGIQ010000085        11378 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000085 DAEGIQ010000000
VERSION     DAEGIQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.087"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(121..1005)
                     /locus_tag="KMT45_002067"
     CDS             complement(121..1005)
                     /locus_tag="KMT45_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBE7005521.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(1092..1991)
                     /locus_tag="KMT45_002068"
     CDS             complement(1092..1991)
                     /locus_tag="KMT45_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBE7005522.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(2008..2514)
                     /locus_tag="KMT45_002069"
     CDS             complement(2008..2514)
                     /locus_tag="KMT45_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBE7005523.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(2703..3323)
                     /locus_tag="KMT45_002070"
     CDS             complement(2703..3323)
                     /locus_tag="KMT45_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBE7005524.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(3399..3836)
                     /locus_tag="KMT45_002071"
     CDS             complement(3399..3836)
                     /locus_tag="KMT45_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBE7005525.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            3999..4829
                     /locus_tag="KMT45_002072"
     CDS             3999..4829
                     /locus_tag="KMT45_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBE7005526.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            4813..6180
                     /locus_tag="KMT45_002073"
     CDS             4813..6180
                     /locus_tag="KMT45_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBE7005527.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            6199..7008
                     /gene="yidA"
                     /locus_tag="KMT45_002074"
     CDS             6199..7008
                     /gene="yidA"
                     /locus_tag="KMT45_002074"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBE7005528.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(7051..7494)
                     /locus_tag="KMT45_002075"
     CDS             complement(7051..7494)
                     /locus_tag="KMT45_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBE7005529.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            7628..7999
                     /locus_tag="KMT45_002076"
     CDS             7628..7999
                     /locus_tag="KMT45_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE7005530.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(8168..8950)
                     /locus_tag="KMT45_002077"
     CDS             complement(8168..8950)
                     /locus_tag="KMT45_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBE7005531.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(8947..9918)
                     /locus_tag="KMT45_002078"
     CDS             complement(8947..9918)
                     /locus_tag="KMT45_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBE7005532.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            10010..>11378
                     /locus_tag="KMT45_002079"
     CDS             10010..>11378
                     /locus_tag="KMT45_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBE7005533.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVK"
BASE COUNT         3366 a         2304 c         1985 g         3723 t
ORIGIN      
        1 aaaattggtg gacagttttt tgttgtacct gtatagatat tgttaagcct ttgcaagtaa
       61 ggtagttatg tgttcaacta cttattaaaa tgtgttattt attaaagata cttgtctcac
      121 ttactgaact ggccgatgat cgtttctgcc ataatagttc tcgacagccg ttggatgccc
      181 taataaatcc ccaactgtcg tgatgcgata cccttgctct ttcagataat cgatcacttg
      241 aggaacggca cgaatcgttt caggatagat atcatggaat aaaataattg ccccatcgta
      301 tactgttgct tggatcttct gcaaaatcaa atcaggattt cttgtcttcc aatcttctga
      361 atcaacagac cattcgatga tcggtcgctg gatgatcgtt gctcctacat tcgtgacaga
      421 accatagggc ggacgaacaa aagtgggggt tttgccagtg acttttttga tctcagtgtc
      481 tgctttcgtc acttcctccg tcatcgactg ggcagaaaga gtggccagat tctgatgatc
      541 gtatgtgtga ttgccaatct cgtgccctgc ttcagctgtc tgcttcacga tttcaggatg
      601 ctgcgccgta ttttctccta agacgaaaaa agtggcatgc acgttttttt cttttaagat
      661 tcgcagaagc tgcggtgttg tggtcgctcc tggtccatca tcaaagctga ttgcgacgct
      721 tttgacagcc ggatctcggc tcgtttttgt actgtcttgt gttgattgtg atgactgact
      781 ttgtaccttt tccgctgatt cttgaatggc ttctttgacg ttagctgttt cagttgattg
      841 gcttttaatg gttgattgtt ctgattgttt gaaagattgt gacgaatgct ccgaagagtg
      901 tgtttgtcct tttacttcgg tgatcaagga ggccaccgaa gtaatttcga aaaacaaaag
      961 cacggataaa gcaataatac tgataactaa ataatttctt ttcactatat ctcttctttc
     1021 tctttttctc ttcttattat ttacttgtac ttacttgaaa attgaaactg ctagtcgcaa
     1081 ctacttctcg tctaatgatc caaataatct tccgatgcca aaatcgtttt tgtcacggac
     1141 ttaggaatga caaattctgg tgtacccata taaccaggag caatttcgta ttgagaaaaa
     1201 gtcaaaacta attgatgctc tttgttcaaa taaaaacttc gtgtaggcgt aacaagtgtt
     1261 ggtttttcta tcgtcccttc ttgaatatct tgctcagtcc aatagtactt agatggatct
     1321 tccttgactt gttcggccat ctgccgtttg acttcttttg tcaaaacaga tagataggtg
     1381 tcatctttga ataaaagtgg aagtgagagc tggacactgt ttttcttgtc gattgtgtcg
     1441 tatctttttt caacatggct atctgctgct gtttgggtga tctgcctttc aatcacgaga
     1501 aaccggttat cgtctacgat tttctgataa tctcccaaaa cttggtaatg atccgtttca
     1561 aaatttccat attctttttc agctttttca aaatctgctt gtccttcgcg aaagtatttt
     1621 tgatttaatc catgaattgt tttattttct ttctcatttt gtgtattgat cttcggtacc
     1681 tgaatatcga ttttttcaga tcgatcagta aagcgttcgc ctaagatcaa ttcaatgact
     1741 gatcctaaga tgggtaaatc ttctgcaaag cttcgaactt gactattaaa aagaaggcta
     1801 cttgttgtaa tcattacgat agccaaagat actccgaacc cttggagacg tctctttctc
     1861 cgctttgctt ttataaatga ttgctcttcg ataataaaac gctggcggat ctcctcttta
     1921 agccctttgg gaattgcttg ttctttataa gttttcttta gtttttttaa attttctttt
     1981 tgggaataca tggcactctt ctctccttca ttctaggatt tgacgtaaag cattcaatga
     2041 gcggtacatt ttcgttttaa ctgtgttttc attcatatcc aagatagtcg caatttcttt
     2101 caaacgaaat ccttcaaaaa agtagaggat cagaatctct ttttccggac tgtctatttt
     2161 atctaaaatc atatggagct ctgcaacgga agtttctaaa ggtaactgtt gggggatatc
     2221 ttgaatttca aataagtcca caggcgtcct tttttgtctg cgccaactat ctaatgccgt
     2281 gcggatcaat atccgataaa accatgcact aaaatgctcg atctcttttt ctttttcaaa
     2341 atagttcagg gctttatgaa aactatcttg catcacatct aaggcctctt ctttatcatg
     2401 tacgtaattc atcgtgatac ggaacatttt ttgataatca gtttcgatga gtccattcag
     2461 caggtgacgg tatttttcga tctgccgttt tttcttatat ccagtaaaaa tcattctcca
     2521 ctcctttctg cctcacttaa tatgacgtac aaggtgcgct aaaagtttca atttttttat
     2581 tttttatgta tttacttggg atttttatat acaaaaaaga aggtgtgaca aaaatcgtgt
     2641 cacacctcct tattctgata ggctgatcgt ctctgtcaca acctctcgta agaaaataat
     2701 gtttattctt tttcagagtc agaaaattct tcctcttcat aatcttcatc atcatcgtct
     2761 tcgtctatga tacttaaatc ttcttcgata tttcctggca agtcttcttc atcatctgtc
     2821 gggtcatctg ctccgatttc ttgcaagtca gaattataag cagcgatttc ttcatcttca
     2881 tcatcatcgt catcaaacag atcatcgtca tcttcttctg tcagatctgt atcttctggg
     2941 tcatcgtcat tgtaatcgat cgcgtcttca tcatcattga taaaggcgtt gactttcttg
     3001 cgtttacggc gacgtggagt atcttcatcc tcttcgtcca ttccatggtt tacttcttca
     3061 tcgatcgaat cgatagcata ccatgaacgc aagccccaac gattatcccc taatgagata
     3121 aagctgccgt caatattcaa gtcagtataa aattgtgcga gtgaatcacg aatttcactg
     3181 tctgatttcc caagatagtt ttggatctgg ttagctaaat cagaaaaatc catgacatcg
     3241 ccacgttgtt ctaagatcgc gtgtgctact tcgatcattg ataattcgct tttgtttgca
     3301 ccttcaaata cactaatttc caatcaggac acgtcctttc acagtctact ccttatcata
     3361 caaaatcaga cacaaaaaat caaacttttc ttttatattt acgctgtaaa atccaaggtc
     3421 aaataatatt cgccgatttt ttccggtccc atgaacagat cataatcgat tcgaaccttc
     3481 cctgctgtgg gacggtcttt caaactaaca tgcagttctt tcgtaaaagt agcaatctgg
     3541 atcataccgt atggtgttcg ataggatgtt tctaaccgtt ctttatagcc aaatttcaaa
     3601 cgcatccgca tttctcctgc acggatcagc tgtacatgac catctggaac taatttgatc
     3661 gttacaggaa tctcatcgcc cgtctcctcc tgaatctctt tataacgtat gtacagcgta
     3721 tcgccaattg tcaccatttg ccctttcaaa tcaaaataaa aatcttgcac ttccccttgc
     3781 tgttttactt ttgtacgcaa tttgattgaa atcggtgttc cttgtgataa ttccattatt
     3841 cattctcctt tacttctcat agctacattg ttcctagttt cttttgtttt gtcaaccaac
     3901 ttgtccattt ttacagaaaa ccttttttcc aaaacaactc tttgatttta gagtataata
     3961 ttgtagaaaa tatgaattta tacaaaggag ccggttgaat gaaccctgcg attcgactag
     4021 atcagcacat ttatcaaaaa aacgactttt ccccttttgc attaacagat atattaacgg
     4081 aatattctaa agaacaccag caaatctttt tacatttctg gcaatatgac tgcacagtca
     4141 tcttaggtat gaaggatgtt cgaactcctt ttctaagtga cgggatccaa acactggaag
     4201 atgcaggtta cgctccagtg atcagaaatt caggtggatt agggatcgta tcagatgaag
     4261 gaattttgaa tatttctttg attttccccc aaagtgacac aaaaaaaata accatagatg
     4321 acgcttacga gcaaatgctc ctattgacta aatacgcttt tccaaaggca ccgatacaag
     4381 cattcgaaat cacccattct tattgtcctg gaacttatga tttaagcatc catggcaaaa
     4441 aatttgccgg tatcgctcag cgtcggatca aaaatgggat ttctgttatg atgtacttaa
     4501 gtgtcaacgg taatcaggaa aaacgtgggc aagtcatgaa gcgattttat caatctgctt
     4561 taaaagaaga ctttggtaca aatggctatc ctgcagtgga tcctgctaca atggcaacac
     4621 ttgaagaact gcttgaccaa cccctttttg taaaagaagt caaagaagca tttgcaaaag
     4681 cttttgaaaa gctgtattct gcaagtgatt tacaggattc taccgactgg ctaaaaaaag
     4741 aaatccagct ggagcaatgg aacaaccaaa tccagagaat ggaacaacgt aataacataa
     4801 aggagctaca ttatgacaac cccttataaa tatcaaatgc tgcctatgga aaaagttttt
     4861 cgtgatccag tccataacta tatccatgtc cagcatcaag taatcttaga tttgatcaat
     4921 tcgaaagaag tccaacgtct acgacgcatt aaacaattag gcacttcttc tttcactttt
     4981 cacggagcag aacatagccg ttttactcat tcattaggcg tttatgagat ttcaagacga
     5041 atctgcgata tcttttctcg aaatttttca aaagaaaaaa taggcaacgg aggctgggat
     5101 gatcaggaac ggctggtgac tttatgtgcg gcactgttgc atgatgtcgg tcatggtcct
     5161 tactctcata catttgaaca tatcttccat acgaatcatg aagccatcac tgtagagatc
     5221 atcacttctc cagaaacaga agtgtatcag attcttaacc gagtagaaga aggttttcct
     5281 gaaaaggtag ctagtgtgat ccaaaaaact taccccaacc cacaagttgt ccaaatgatt
     5341 tctagtcaga tcgatgcaga tcgaatggac tatttgcttc gggatgctta tttcactgga
     5401 acagaatatg gaacatttga tcttacccga atattacgtg tgattcgtcc ttatgaaggc
     5461 gggatcgctt tttccatgag tggcatgcat gcagtggaag attatatcgt tagccgatat
     5521 caaatgtatg tgcaagtgta cttccatcct gtttcccgtg gtatggaagt aattttggag
     5581 catctgcttc atcgcgcaca cgagttgtat caagaagatg ccagcagttt tgaactccat
     5641 tctcaattac ttctgccatt tcttgacgga agctttactt tagaagaata cctcaaactt
     5701 gatgatgggg tcttgggcac ttactttact caatggtctg aatctgctga tcctatttta
     5761 aatgatttag ctaaacgttt tttgaatcgt aaaccactga aatctgctac tttttctggc
     5821 aaccgggatt caaaattagt ccaagaactc actttgttag tagaaaaggt cggatataat
     5881 cctgtttatt acactgctgt gaattctagc tacgatctac cttatgattt ttatcgccca
     5941 gaacaaggca gacatcgcac acaaatcgag attttgcgaa atgacggaac tttgatagaa
     6001 ttatcacagg ttagtcagct tgttgctgca ttagcgggac aagaacaagg agatgagcga
     6061 tttttctttc caaaagaaat ggttgatccg tccttgcgtg atcattatga tttatttgac
     6121 gaaacttatc aagaatttgc cagccatatc cgtaatggcg cattgattga aataaattaa
     6181 aatatattgg aggaaattat gtctatcaaa ctagtcgcta tcgacatcga tggtacttta
     6241 ttaaatgaga aaagagaagt aacaaaagaa gtaaaagaag cgattgccgc tgctgttgca
     6301 aaaggtgttt cgatcgtttt atgcactggc cgtcccttac caggtgtcca ggaacagtta
     6361 aacgagttga acctttttca agataatgat tacgttatta cttataacgg ggcactcgta
     6421 caacaaacga aatcaggtaa aatcatcgcc cgtcacggac tgacacatga agatttccta
     6481 gagatcgaag taatggcacg ccgtgtcggc agtcatctcc attcgatcga tgacaaagcg
     6541 atctacacgc ctaatcgaga tatcagcgct tatacgatcc acgaagcaag tttagtcaag
     6601 atgccgctaa aatatcgaac acctgaagaa atgacaccag acatgaacat cgtcaaaatg
     6661 atgatgatcg acgaaccaga aattttagat gcagcaattg cgcgtttacc gcaaactttc
     6721 cgtgataaat acacaacggt caaaagtgcg ccctattact tcgaagtact aaataaagaa
     6781 gcaagtaaag gtgcggcagt tgcgaatctc gcacagcacc ttggtattga tcaagatgaa
     6841 atcatggcta tcggagataa cgagaacgac ttatccatga ttgaatatgc tggtctagga
     6901 gtagcaatgg gaaatgccgt ttctcttgtc aaagaagcag caaatgtcat cacttcatca
     6961 aacgacgagc acggtgtagc tgaagcaatc aaaaaatatg tactgtaata aataaaaaaa
     7021 ctgagagaaa gctgatctac tcgctttctt tcagttttta tttttttcga tccatctctc
     7081 gactgcccat cggtggctac cacgtttcaa tttcacaagc gcttcttgag ggctcaccca
     7141 ccaaatttta ttggtctttt cagttggttc tcctatacgc ttccaagaat cagcaacaaa
     7201 gaaatatcct ggattataat aataagttgc acgataattc gagtaaaaat actcatcagc
     7261 ttctcccaga taagaagaaa tttctgccac aattcccatt tcttctatca gctcacggac
     7321 aatcgcttct tctttcgtct cttctccttc aatttccccg ccaggaagaa aaaaagcacc
     7381 atttggagcc tcgattactg caatctgctg atctctttct atgacaatat atgctgcata
     7441 acgggtttga taatcagcaa attgcttttt ttcaccaaaa attggtttat ccatgaatag
     7501 tttccacctt aacagtttta ttttctatcc attttacatg agaaatttct catgaacaag
     7561 cacaaatcaa ctatttcctt acttcttttt gttatgattg agtaaaagga aaaggagaga
     7621 tgaatctatg aaaatggctc acacatgtgt tcgagtgaaa gatcttgaag catcaatcga
     7681 attttatcaa aaggcatttg gttttgaaga aagccgccgc cgtgattttc cagaaaacaa
     7741 atttacactt gtttatttga cacttcctgg ggatgactat gaattagagt tgacttacaa
     7801 ctatgatcat ggtgcgtatg atctaggaga cggctatggt catatcgcta tagcagctga
     7861 tgatctagaa aaattacacg agcaacataa agctgctggt cttgagatta ctgacttaaa
     7921 aggattacct ggcactgcgc cttcttacta ctttgtcatt gaccctgatg gttataaaat
     7981 cgaagtcatc cgaggatagt tagtatgggg cattttactc ttgattgaat aaaaaacaaa
     8041 caccaataat taaaatgaga cgcaaacttt tcattaaatt gaaaaagttt gcgtcttttt
     8101 ttaacaacca ttgtagctat aaggttgcca ctctccacca atttattttt aattcatttg
     8161 aaagaaatca aataaacgag gtacctttag aaaacaaaac tgcttttcct cctattatca
     8221 cacgatcttc ttttaattca cattctagat ccccgcccct tgatgacatt tgataagctg
     8281 acaattttgt tttattcatt ctttctgccc aataaggaat aagattagaa tgtgctgatc
     8341 cacatactgg atcttcattg attgttaatt ttggaaagaa ggttctagaa acaaagtctt
     8401 tttgcacact ttctgctgtc acgatgactc ctattcctaa ttcaaaattt ttgatagctg
     8461 taaaatctgg ctgcaattta gccaccgttt tttcatcttc taatacgaaa aataaatcac
     8521 gtgctaagta ggcttcttta atcttagccc caatcgcttc ttcatattca gacaatatcg
     8581 gcactttttt cggcataata ctaggaaaat ccatgtaata ataatcttta attcttgtca
     8641 caaatagatt accgctttga gtagaaaatt taatcgtttc atcaggtatt ttgtaatagt
     8701 taaataaaac aaatgctgtt gctaatgtcg cgtgaccaca aagatcgatt tctcgatctg
     8761 gcgtaaacca tctaagctca aattcttggt ttttcttcac tgtaaatgct gtttcagata
     8821 aattgttttc aatggcaatt ttttgcatcg taccttctgg cagccattct tctagcacat
     8881 aaaccgctgc tggatttcct ttgaacacct catctgcaaa tgcatcaact acgtaataat
     8941 ctaagttcat tttaaagccc ctttctctaa tttgttataa atatattctg cacaacaaag
     9001 atcttcaata gctagaccaa ctgcgtcgaa aatcgtcggc ctcgattctc ctttataaaa
     9061 tgtttttttc tcagaaacta attcgcctaa tgtaccaaca atacaagatt cctctaaatc
     9121 tccttctgcc atggctataa gcaggtcccc gctttctgtt aaggcagctg agtaatcatc
     9181 tacatagatt tgagaagttt ccatcaacga tgtacttatc tctcttgtat caggagtaaa
     9241 tgtgcccaca gcattgatat gtgcgtcttc ccttacccac tctttttgaa gaaatgcctc
     9301 tttactagag gtcaacgtac aaatgatttc cgctggacgt actgcatctt ctactgatat
     9361 tgcatttata aacgttattt taggatactg ttttttagct ttagcgataa aagtatttcg
     9421 tctttcttct aaaatatcgt aaacaaacac tgtagtgatg ggacgaatgg tagaaatcgc
     9481 agtcaaatgc gacaatgctt gttgaccagc tccaattaaa gcaagtgttt ctgcatgcgg
     9541 atttgctaag taatctgttg ccagcgcaga tacagcagct gttcgaatcc aggtgactgc
     9601 atttgcgttt atcaatgctt tgggtagtcc gttttgagca tcaaacaaga aaatctctcc
     9661 taaatgagaa ggtaaatgat actgatgatt atccggaaaa gcggtaatga cttttgtgcc
     9721 aaaatatctg ttctccccca aataagcagg catcatcgca aacatatttt tgccttttcc
     9781 gtcaggtaaa atttccacca atcgaggatt ttggctgatt ttaccacttt cataatcaaa
     9841 aaaacatttt ttcattactt gaatcgcttc ttcaaaacta aaagaagcta ctacttcctc
     9901 aaaattaata attttcatag acaattccct cctcacaaaa gattaacttc taaaatcaat
     9961 acaatcaatg gtaaaattgt atggtataca attttaagga gtggagaaaa tgcctattaa
    10021 ttcttatgac aattacccgt taacttggcg accagacaaa caaaaattga agcgtcctta
    10081 ttatcgatcg ttgattcaac aattagaaca agatatttta aagaataaat tacagaaaaa
    10141 cactcagtta ccctcccaaa gagagctagc tgattattta gatttaaatt ttactaccgt
    10201 tggcaaggct tataaatatg gaattgagaa aggtttgctg tatacgaata ttggaagtgg
    10261 cacatttatt tctcccaatg tctttgagtc catcaccatc tctaccaatg aagtaaccga
    10321 acatatcatt gattttggtc tggttagttc ttttgaagaa tgtaataaat tcgttgtccc
    10381 gtttattcaa tcaactagta ataacccaaa tcttgttggt ttgcttaatt accaagatcc
    10441 caaaggcaca aattttcaat tgactactgc agcaaattgg ttaaaaactc aaggagtcat
    10501 tccgtcctta gaatctctat ctattgtttc cggtgttcaa aatggattgg ctatcacact
    10561 ttctgcactt ttctctccag gtgatagaat agcaacagac cgatatacat acgctaattt
    10621 cattgaattg gctcagttat atcatttaga gatcgttcca attgattttg acgagcaagg
    10681 aatgcgacct gatatattga ttcaggaatg tagaaaaaag aagattcatg gtgtctttct
    10741 tatgccttct tgtaataatc ctatagggtt tcaaatctct cacaaaagac gagagcaatt
    10801 agccgaagtg attgaaaaag aaggtctatg ggttattgaa gatgatatcc attcattttt
    10861 aacaacttac tatcaacata cgatcattcc tcctttccaa aatttattac ctaaccaaac
    10921 aatctactta gctggaatga caaaatttat ttgttctgga ttgcgagttg cttatcttgt
    10981 agttccagta catgtccaaa gcaagataga acaagcaatt tttaatataa atgtgaaaac
    11041 ttctggatta gatgtggaag tcatcactca aatcctccat tcaaatgaag caaataggat
    11101 cttagaaact aaattaagtc ttacaaaaag agccaatcag ctttttgatt ctcttttcaa
    11161 agtagaaaaa ccaacgaatc gtgtccctta ctatcgaatt cttccagttg atcctgagat
    11221 tgaccctaag ataattgaga atgactttct tgctaacggt atccgacttt atcattccag
    11281 acgctttact acacgtgcgc aaccagatgc attcgttcga atttccctat cctctaataa
    11341 ccttgaaatt ttagcttctg gactaagtat cgtaaaag
//
LOCUS       DAEGIQ010000086        11366 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000086 DAEGIQ010000000
VERSION     DAEGIQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.088"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(58..285)
                     /locus_tag="KMT45_002080"
     CDS             complement(58..285)
                     /locus_tag="KMT45_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBE7005534.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(388..2976)
                     /locus_tag="KMT45_002081"
     CDS             complement(388..2976)
                     /locus_tag="KMT45_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBE7005535.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(2996..3655)
                     /locus_tag="KMT45_002082"
     CDS             complement(2996..3655)
                     /locus_tag="KMT45_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBE7005536.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="KMT45_002083"
     CDS             complement(3704..4207)
                     /gene="trmL"
                     /locus_tag="KMT45_002083"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBE7005537.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(4428..5294)
                     /locus_tag="KMT45_002084"
     CDS             complement(4428..5294)
                     /locus_tag="KMT45_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBE7005538.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            5403..6044
                     /locus_tag="KMT45_002085"
     CDS             5403..6044
                     /locus_tag="KMT45_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBE7005539.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="KMT45_002086"
     CDS             complement(6106..7467)
                     /gene="mgtE"
                     /locus_tag="KMT45_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBE7005540.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(7485..8384)
                     /locus_tag="KMT45_002087"
     CDS             complement(7485..8384)
                     /locus_tag="KMT45_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBE7005541.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(8386..9183)
                     /locus_tag="KMT45_002088"
     CDS             complement(8386..9183)
                     /locus_tag="KMT45_002088"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBE7005542.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(9161..9865)
                     /locus_tag="KMT45_002089"
     CDS             complement(9161..9865)
                     /locus_tag="KMT45_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBE7005543.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            9996..10571
                     /locus_tag="KMT45_002090"
     CDS             9996..10571
                     /locus_tag="KMT45_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBE7005544.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            10689..11366
                     /locus_tag="KMT45_002091"
     CDS             10689..11366
                     /locus_tag="KMT45_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBE7005545.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         3232 a         2471 c         2040 g         3623 t
ORIGIN      
        1 ttttttcaca tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca
       61 aggattcgca gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg
      121 cgccttgtct aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg
      181 actttcattc aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga
      241 ttcttttgct aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc
      301 ttttgatttc agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc
      361 atttttagct atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg
      421 agaactgttt tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt
      481 aagaacgaaa tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa
      541 tgaagaaaac agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc
      601 actgtcatct tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc
      661 atcttcaaat ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag
      721 agtgatattc attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc
      781 tgattcattc gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc
      841 cttactgcga gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg
      901 gaccatgggc aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca
      961 ataagaaaga gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa
     1021 ctggataact aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat
     1081 ttctcccata tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt
     1141 gtctcctatc cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg
     1201 attgaatatc tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat
     1261 tggtgctaat acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat
     1321 tttagagatc aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg
     1381 attctttgtg aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat
     1441 actagaacca tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat
     1501 ttccaaaaga tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc
     1561 tactaagata acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt
     1621 atcgaccata gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac
     1681 tgaaggtgtt ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag
     1741 acctgtcgtt tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg
     1801 aaaaatttct tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag
     1861 attgacgatt ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa
     1921 tagaggggaa cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc
     1981 gaatattttt tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc
     2041 ttttttacga ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa
     2101 actgttttcg taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat
     2161 aattacagta gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg
     2221 gatcgtttgc tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg
     2281 ctgaaaaatc tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc
     2341 gatttgttcg gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag
     2401 ctggtacgga ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact
     2461 gaaagctaac tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc
     2521 gtggtttagc cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat
     2581 ttgttttaat accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc
     2641 tacaattttt tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc
     2701 tacgatccca tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct
     2761 tccgtattta ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat
     2821 atcgccgaaa cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact
     2881 gatgtgtacc aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac
     2941 tgtaccaacg aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata
     3001 gtaaaggttc tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt
     3061 catatggcaa taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca
     3121 atccacccat ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag
     3181 ttaaagaagc tccatgtcct acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat
     3241 ctgctacaac agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag
     3301 aaggatcata cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt
     3361 acatttcatc gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg
     3421 tataaaaaat ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg
     3481 ctcgctttgc tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa
     3541 atgctttgat ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa
     3601 aacgtctttc ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca
     3661 gcaaactcct gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat
     3721 tttcatagtg atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca
     3781 ctatcattgc agctgtattc gataaattca atgagcggac atgctcatca ttcataggga
     3841 tgcgtaaaca cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt
     3901 ttccaaataa aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat
     3961 gggcaaattt tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta
     4021 aattactgtg gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat
     4081 gtttgtcatc agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg
     4141 cacacgtacg ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat
     4201 gattcatcag gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt
     4261 tttgcataaa aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga
     4321 agctcttctc actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac
     4381 ttgttgctaa tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta
     4441 gaaaaacgtt ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa
     4501 ggattcttat ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg
     4561 gccaaaagat ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt
     4621 tcagttaatt tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt
     4681 ttttcaggtt gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc
     4741 accaccttca acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa
     4801 ggcgaaaaga tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa
     4861 tcagctacac accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt
     4921 gaaatatcaa atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag
     4981 ctaccttctc cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata
     5041 gtctcaataa cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt
     5101 ccttgatcgt attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa
     5161 aggtcaaaac ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct
     5221 tggcaaaccg gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca
     5281 atttttttta acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt
     5341 ggcaactgct aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt
     5401 agatgatcaa agaattttgc gctgaaaact acacatctat tcctctagca attgctagag
     5461 gagccgatcg gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg
     5521 gtgtgatcga agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta
     5581 ttcgtcctag agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg
     5641 atttgattga agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt
     5701 caggctggct agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga
     5761 tcaccttcca tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt
     5821 ggctggtaga acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta
     5881 ttgtagaaaa tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca
     5941 ttttgcctgg tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg
     6001 taagcgaagt acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa
     6061 acatttgcaa caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc
     6121 cataaataaa gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt
     6181 gataaaggga ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat
     6241 taaactacct gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa
     6301 ccccaagata aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac
     6361 agctcctgtg acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc
     6421 tttctcatca gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt
     6481 accggcagtt cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa
     6541 gtcttcatag tgactgatta aagaagcggt cgacatacct aaaaacaaca gagtgatcag
     6601 ccaaggcaaa cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac
     6661 cccggcaagt cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc
     6721 gactgtgacg attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc
     6781 ataatctcta aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac
     6841 tcgttcgctc atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag
     6901 agaaataacc cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc
     6961 agctacatct gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac
     7021 gatcgaaaca aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag
     7081 ttccttgatt tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt
     7141 tttatccagc gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc
     7201 agctgcatag ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc
     7261 aatcacatcg aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct
     7321 gttctcttct gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa
     7381 actgtttctg aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct
     7441 ttcctctaat tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt
     7501 ctcacaactg cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta
     7561 aatggatggt aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc
     7621 attcgcccgc catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact
     7681 cttatttgat gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt
     7741 cgcttctcta tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc
     7801 aatagcgaag aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct
     7861 gaaagccttc cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc
     7921 atatctaatt ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaagcc agatgtatcc
     7981 cgatctagtc gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa
     8041 gcctttacac gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg
     8101 cctgctggtt tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt
     8161 tcatccaaaa gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca
     8221 tccttagcta gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct
     8281 tgaaatttta ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt
     8341 acttgctgag aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat
     8401 aaaggcatct ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg
     8461 ttcttctgct attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc
     8521 aaccgttaca agataatctt ctgtattttc caattttatt tctagccatt catcatgggc
     8581 aatcacgatc ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc
     8641 caactgaaag gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc
     8701 tgtcgaacca gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc
     8761 gtttttgatg tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt
     8821 cagtgctaaa aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa
     8881 gggataacta acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc
     8941 gtaatcccgc caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac
     9001 atcattcaga caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac
     9061 tgagatgacc aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc
     9121 gttcaaacgt tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt
     9181 catcccctgc ctcctctctt tcgctttctt tcttcaaaag cgttcgatag tagacatcat
     9241 ctgattttcc tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac
     9301 tggacatctc ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca
     9361 tttcttctgg aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga
     9421 aattcatcgc aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca
     9481 gctgtacagg atactcgatc accagatgat aagaacggta gccacttgct tttttatttg
     9541 tgatgtaatc tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa
     9601 cttgatggat atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt
     9661 cttcttctaa acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga
     9721 ctggcttgac tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact
     9781 gtttgcggat cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta
     9841 aaaatgcttc ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt
     9901 attctaccat atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga
     9961 aaattaggca aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat
    10021 ataaatctat gctgacaaag caagagtacg agatattcat cgctcactat caattaacag
    10081 ataaagattt ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga
    10141 aacaaaattg tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa
    10201 cacctgctga aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag
    10261 catttatctt cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt
    10321 tgaatatcga tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgagctgaat
    10381 tccctattga agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt
    10441 ttgaactgga actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt
    10501 taaaacgttt ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag
    10561 ctaaaaatta gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc
    10621 tgttaagtta taggatgtga aagaatctag tcactagttg tatagaatct attttggggg
    10681 agattcatat gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa
    10741 tggaacaaca gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga
    10801 tgcaatttcg ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa
    10861 aaggaatttc tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg
    10921 cagcattgga ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga
    10981 tcaaattgca agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat
    11041 ccatatttga agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact
    11101 tcgttaaaga agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac
    11161 acccttctgc agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag
    11221 taaaaggtac aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat
    11281 atcaagtttt taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta
    11341 gccatttaca cttgctttct aattaa
//
LOCUS       DAEGIQ010000087        10861 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000087 DAEGIQ010000000
VERSION     DAEGIQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.089"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(12..1817)
                     /gene="glmS"
                     /locus_tag="KMT45_002092"
     CDS             complement(12..1817)
                     /gene="glmS"
                     /locus_tag="KMT45_002092"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBE7005546.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2227..2517
                     /locus_tag="KMT45_002093"
     CDS             2227..2517
                     /locus_tag="KMT45_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005547.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2530..3555
                     /gene="sppA"
                     /locus_tag="KMT45_002094"
     CDS             2530..3555
                     /gene="sppA"
                     /locus_tag="KMT45_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBE7005548.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3558..4217
                     /locus_tag="KMT45_002095"
     CDS             3558..4217
                     /locus_tag="KMT45_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBE7005549.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4374..5306
                     /locus_tag="KMT45_002096"
     CDS             4374..5306
                     /locus_tag="KMT45_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005550.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5460..6749
                     /locus_tag="KMT45_002097"
     CDS             5460..6749
                     /locus_tag="KMT45_002097"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBE7005551.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            6996..8246
                     /gene="clpX"
                     /locus_tag="KMT45_002098"
     CDS             6996..8246
                     /gene="clpX"
                     /locus_tag="KMT45_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBE7005552.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8329..8916
                     /locus_tag="KMT45_002099"
     CDS             8329..8916
                     /locus_tag="KMT45_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBE7005553.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(8990..9145)
                     /locus_tag="KMT45_002100"
     CDS             complement(8990..9145)
                     /locus_tag="KMT45_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005554.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            9281..10609
                     /locus_tag="KMT45_002101"
     CDS             9281..10609
                     /locus_tag="KMT45_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005555.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            10620..10829
                     /locus_tag="KMT45_002102"
     CDS             10620..10829
                     /locus_tag="KMT45_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005556.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         3613 a         1869 c         2247 g         3132 t
ORIGIN      
        1 ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt gtcaacgtcg
       61 aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac tgtggttaga
      121 gtagttagat aagggtggac agttggtagc accagctggt cactttcctt ttctaatccc
      181 tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag gtttcctcga
      241 gtatgcgcac cagtcacttc ttcactgatg attccaatca ctggtgttcc ttcttcgatc
      301 aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg gatataactg
      361 atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcgcc acgtccaatg
      421 taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa ataatctttt
      481 tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt gatatcgaat
      541 acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa agttagtaca
      601 gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc agcatgcaat
      661 agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt aatcgtaagt
      721 gaagggtagc cgagacggtt gatttttacc agaacttggc ggctatctgc tgtttcgcca
      781 ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt atagccaaat
      841 tcactggcta aatgaacctc aacagggatc tgtgtcaatt tttccagagt ctgtttacct
      901 gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc gctatcagac
      961 aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac gtatttttga
     1021 gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata aaaaggatag
     1081 gtacctttct cgatatctga agaatctact tgtgcttcga atgaagaacg ttctacgatg
     1141 tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat ctcttcatct
     1201 tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct ggcaatcaca
     1261 ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac gtaaataaca
     1321 tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac ccgcaaagct
     1381 tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag atgagcgata
     1441 atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat tttaagttct
     1501 tcaaagtttt cgattacgcc attgtgaacg agcacgaagc gtccactttg ggaagtatgt
     1561 ggatgggcgt tttcttcaga aggttgcccg tgagttgccc agcgtgtatg accaatacct
     1621 atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct gccttgtgct
     1681 ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc ataaccacgg
     1741 tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa tccaatcatt
     1801 ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta tgaatgcttc
     1861 cagctgcagg aaaatccttt tgttttatct ttgcagatat tttttgtgga ttttctgtag
     1921 agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc gtcatctgtt
     1981 tacacagacc tggcgctatc ctaaaaggta taactatcct cctcttttgt tttttatctg
     2041 gaaacattta aaatcataac cttttgcgaa tgttcttgtc aacaaaaaaa gaataaaaaa
     2101 taaattggta tatatcaaat gctttgtcaa atgctctttt tatgtcaaaa aataatgggt
     2161 ttttatctat catctttttc aggagttcgg tacaatagaa agtaatcgga tagaggagga
     2221 gtaaaaatgg attcaaaaat tgagatcatg acactgggaa tgttaaaaaa acaattgtcc
     2281 aaattcgaag catcagctgg tgtatcagat gatacgaaaa tatttttaga tactggctgg
     2341 gatagcatcc aagaaatcgc acctgatgcc ttggaagttg ctgaagctcg tgagtttacg
     2401 gtagaagatg aattgacaaa agagtcgttt tccgggtatg cgcgggaaga aaaagcagag
     2461 cggttcgaca cttctgagca gtcagaaact gtgatcgtca taaaaaactt gtattagaaa
     2521 ggagaaagaa tgaataagag acgttgggca gcagttctga ttgctgcagg attatttgtg
     2581 gtttcgttag tttcagctag tttgactaag cctcaggaaa acgatcaaat tgagggattg
     2641 aacagttggt tatatgggga tgaagaacta gcttcaaatg tgttggaaga gggagacagt
     2701 acgcaaaaaa tcgtaaaatt atcagtagat ggaacgattg ctgcgggagg gtcttcaggt
     2761 ttatttgctt ctgaaggtta caatcataaa aattttatgg aacaactgaa ggcgattgaa
     2821 gaagatccaa cagtcaaagg tatcttttta gaagtaaatt ctcctggtgg cggtgtctac
     2881 gaaagtgcgg aaatcgctaa gaaattggat acgattcgta aagaacatga tatcccaatg
     2941 tatgtcagca tgaaaaacat ggcagctagc ggtggctact atatttctgc tcaagcagat
     3001 aaaatctttg caacagaaga aacagtgacc ggctcaattg gtgtgatcat gtcaggattg
     3061 aattattcag gacttctaga aaaactgggt gtggaagata caacagttaa aagcggagcg
     3121 ttgaaagata tgggttcagg aacgcgacca gagacaaaag aagaagaagc ggtgcttcaa
     3181 gcatatatcg acagcgcata tagtcgtttt gtagcaatcg tcagcgaggg acggaataaa
     3241 tctgaagaag aagtcaaaaa aattgctgat ggaaggattt atgatggtgt ccaagcaaaa
     3301 gaagtaggtt tagtcgatga attgggattt cctgaagatg ctttaaaagc tatgcgaaag
     3361 gaacaacagc ttgaagatgc ggaactagta gaatattctt ccacgccatc tgggtttgct
     3421 agtacatggt taggaagtaa actagcagaa ttccagggat tgaagccttc tgagactagt
     3481 cagatcttat ccgttcttga gaatctaggg acagcggaat caccaaaagc aatgtattat
     3541 tacggaggtg agtaagcatg tcagagcttt caaatgaaaa taaggaacaa gaaaaagata
     3601 agcaacagca gcctgctgta ggacctgact ccaacttttc ccaaaaggta ctgagctctt
     3661 ttgaaagaga gcctttgacg cccgaagaaa tcaaaaagaa acaacgtaaa tggaaaaagt
     3721 atacggacac aagaaatgaa ccgccaaaag caaatgattt tcctaagtat ttctatgcag
     3781 gtttttttat ccgaatgttt gctttcttgg tcgatctttt gtgtatcgga gctattacgc
     3841 gtttaacact tggtattgcc aaaaatctag gttggcttaa tttttcagat tcttatctga
     3901 gtatctacgg attcacagcc ttacttattt atctagcgta ttttatttta ttgacgaaat
     3961 tcaatcatgg tcaaacgatt gggaaaatga tctttggtat tcgagtcatc agctttgatg
     4021 aagaagaact tagctggtca accgttttag ttagagaagg tgcttgccgg ttcattctaa
     4081 aatttccttt gctgatgtta gggtatcttc cagcaatctt taatcagaaa aagcaacatc
     4141 taggcgattt tttcactaat acaagtgttg ttacgatcaa tctgatcaaa gcattcaacc
     4201 aggaagtcaa cagctgatga gcaataaaaa gcagtaacag ttgaaagcac cttctaaaag
     4261 tcagatctaa aatctagctt ttagagggtg cttcagttat ttttaattgt catgcttttt
     4321 ttgttttgct actattgaac atgagctgaa catgagcaag aggaggacac tgcgtgaagc
     4381 cgattgattt tccagaaaaa tatgagaatt taatgcgagt agcacaacaa gcactagcaa
     4441 accagcaata tcagcaggca aaagaattat ttcaacgagc ttacgagttg aaagaaagct
     4501 ttgaggctaa tagcctgctt gttttttgtc tttacgagtt ggacgagaaa aaagaagcat
     4561 tgaagcaagc actcttgcat gaaaaacagt atcttcaaaa tgaggagttt gctgaatttt
     4621 attttgactt gttaatcagt acacaagatt ttttatatgc tagaaaattg atagcaagta
     4681 cagattttta tgaagctttc gagcagcggg tcattgaaaa aatccaattt gcagaagaat
     4741 tatccggaca aatggagagg cagaagatca aggcactcca caataaatcg gaggaacttc
     4801 cttcgttgga acctgcacgt cagcttgcgt ctattgatga gatcgaacag cttccttatc
     4861 atgagtttat ccaaacagcg agtaaactta ttgtatcgcc agaggtccat attttagcaa
     4921 gggcaaaatt actggagaca cttaggcaat tgaacgaatg ccatcctgtt ttctatttga
     4981 cgattgaaga aaagttagtc aaagtcatac caaaagatct gccaaagccg cagcagcagg
     5041 caagttacca gcaattatgt gtattttcag atcgttacgg taatgaagat gcgctgctta
     5101 gttccgtgtt aaatgaagaa ttcacgttgc aatctgcaat tgtttatcct gtctatgata
     5161 catacatcga agacccgaag cgctggtttc aactgactgt agaggcgtgt acaggaaaaa
     5221 cgatgtatga aggtacagaa gatgaaaaac aagatttttt aaaaaaaaga gaaaaaattt
     5281 tgcagcaaat gatcttcttc cattagattt tatcagtgaa aaagataaaa atgttatgaa
     5341 aaacaaggat tctatccatt caatatgttt acaatttgaa agggttagtg tactatttaa
     5401 aggtatgcat gaaacgtttt tgatgtataa aatgaatttc taaactcgga gggaattaaa
     5461 tgactgcaac atttgaaaaa aaaggcacga atgatggtgt tttaaaattt acgattgcgc
     5521 aagacgaaat ccaaaaagga ttaacaactg cgtttaataa agtgaaaaaa agcttgaacg
     5581 tacctggatt ccgtaaaggg aaagtttctc gtcaagtatt caaccgtatg tacggtgaag
     5641 aagctttata tgaagatgca ttgaatgctg tattaccaga agcttatgaa gctgctgtca
     5701 aagaagctgg catcgatcct gtttctcaac caaaaatcga tgttgaaagc atggaaaaag
     5761 gtcaagactg ggtaatcact gctgaagtaa cagtgaaacc agaagtaaaa ttaggacaat
     5821 ataaagatct agttgttgaa aaacaagacc gtgaagtaac agatgctgac gtggatgcac
     5881 gtatcaaacg tgaacaagaa gcacaagctg aattagttat caaagaagat gctgcagctg
     5941 aaaatggcga tacagtagta atcgacttcg aaggctttgt agatggcgaa gcgtttgatg
     6001 gcggaaaagg cgaaaattac tcacttgaat taggttcagg ctcattcatt ccaggttttg
     6061 aagatcaatt agttggtcat aaatcaggtg aaagtctaga tgtaaatgta actttcccag
     6121 aagattatca agcagaagat cttgctggaa aagaagcagt cttcaaagtg acaatccatg
     6181 aagtaaaagc aaaagaagtg cctgaattag atgacgaatt cgcaaaagat gttgatgacg
     6241 aagtatcaac attagacgaa ttgaaagaaa aatatcgcaa agaattagta gaaacaaaag
     6301 aaaaagcagc ggacgatgca aaagatgaag cagcgatccg tatggctgtt gaaaacgcag
     6361 aaatcgttga attgccacac gtgatggttc atgatgaagt acatcgttca atggacgaat
     6421 tcttgaacaa catgcaacgt caaggtatct ctcctgaaat gtactatcaa ttaactggtt
     6481 ctacagaaga agacttgcat aaacaattcg aaggtgaagc agaaatgcgc acaagaacaa
     6541 atcttgtgat cgaagcaatt gcagctgctg aaaatcttca agcaacagaa gaagaaatca
     6601 atgcagaaat ccaagaatta tcagaaacat acaacatgcc aatcgaacaa atcaaacgcg
     6661 tgttgacaga agatatgctg gaacatgatg tgacaatgaa aaaagcaatc gaattgatca
     6721 caggtacagc gacagaagcg aaagaataga aatcaagtaa taaatcattt ctgaagaagc
     6781 tacttctaga aaattgttta ttcgaaataa gggactgtga cacgattttg tcacagttcc
     6841 ttattttttg ttaagagtaa aaaatgcaaa ggtataggtc agttgatcct ttgttaaaca
     6901 ctttttcttt ttactaaact atgttatgat agtaaaagac tgtaaagtct ttgatagttt
     6961 cttgcatact ttttgcaagt taaggggtga agaccatgta tgacaatcca agtagcaatg
     7021 agaccgtacg ctgttctttt tgcgggaaaa cccaagaaga agtgaaaaag atcgtagctg
     7081 gtccaggggt ctacatctgt aatgaatgta tcgacctatg taaagaaatc attgatgaag
     7141 aattttatga agaagctgta cgcgaattca ctgaagtccc taaaccacta gaaattcttg
     7201 aagtattgaa taactacgtg attggacaag atcgcgcaaa acgctcgtta gctgttgccg
     7261 tatacaatca ctataaacgt gtcaatcagc aagcacaaga aaacacagat gatgttgaac
     7321 tgcaaaaaag caatatctgc ttgatcggac ctacagggtc tggtaaaaca tttttagcac
     7381 aaacattagc aaaaacattg aatgtacctt ttgcaatcgc agatgcgaca agtttgacag
     7441 aagctggtta tgtgggggaa gatgtagaaa acattttgct taaattgctt caatctgctg
     7501 attacaatgt ggaacgtgca gaaaaaggga tcatctatat tgatgagatc gataaaattg
     7561 cgcgtaaaag cgaaaatgta tctattactc gtgacgtttc cggcgaaggt gtccaacaag
     7621 ccttgctgaa aattctagaa ggaactgttg caagcgtgcc acctcaggga ggacgtaaac
     7681 atccacatca agaatttatt cagattgata cgacgaacat attgttcatc gtaggtggtg
     7741 catttgatgg catcgaaacg attgtgaaga atcgtatggg cgaaaagacg attggatttg
     7801 gtacacaaaa ccaaaaactt tctgaagacg aaagtgtcat gcagcatatc attcctgaag
     7861 atttactaaa attcggattg atccctgagt ttatcggacg attacctgtt atggcagctt
     7921 tagagaaatt gacaacagat gatctggtac ggatcttgac tgagccgaaa aatgcgctag
     7981 tcaaacaata tcaaaaactt ctttctctag ataataccga acttgaattc gaacctgaag
     8041 cgttgagagc cattgcgaaa aaagcaatcg aacgcaatac cggtgctcga ggacttcgtt
     8101 ccatcattga agaaatcatg atggatgtga tgtttgatat cccttcaaat gaaaacatcg
     8161 aaaaagtgat tatcacaaaa gaagcagctg aattgtctgg tgagccaaca gtaatctata
     8221 aagattcaga gaaaaaagcg ggttgatgaa aagaggctgc cgtaaaagtc attttggctt
     8281 ttgcgacagc cgcttgcttt attcatcacc ttgttaagga gagagaccat gaaagtccat
     8341 caagctgaaa ttatcataag tgcagtggca ccaaaacaat atccagatac acaattgcca
     8401 gaaatagctc tggcaggacg atcaaatgta gggaaatctt cttttatcaa cacattgatt
     8461 gatcgaaaga atttagcccg tacttccggt aagccaggaa agacccagac attgaatttt
     8521 tatttgatcg agaacgcatt acattttgtc gatgttccgg gatatgggta tgcaaaagta
     8581 tccaaaactg agcgagcaaa atggggaaag atgatcgaga catatttaac gactagagac
     8641 cagctacgtg cggtcgtttc tttagtagat ttgcgtcata agccttctca agaagatatc
     8701 cagatgtatc aatttttgaa gtattatgat atccctgtaa ttgttgttgc cactaaggca
     8761 gataagatcc ctcgtggtaa atggaacaag catgagagcg aaatcaaaaa agcgttggat
     8821 tttgatcgca atgacacgtt tattctcttt tcttccgtta caaaacaggg aaaagaagaa
     8881 gcgtgggcag caatcgaaca agcaattgaa tcataaaaag aataaaaaag acttaccagt
     8941 caaactcttt cataaaagtt tggctggtaa gtcttttttt gagcttaact tacttttgtt
     9001 tcttgtcttt tttcttttca ttttgtggct gtggttttag aaatcgatcg atctcttctt
     9061 ttggtgcaac tgttttttcc ggatcgacag caaatgattc gaaaagttta tctaaattat
     9121 ctgtacgttc aaatttcaac cccattgtaa acacctcttt gtttcttttt acttagtcta
     9181 tcatgtaatc gtccaaattg gtttctaaga cataaagttt tagtttttct ttatcgttgg
     9241 ttataattaa attataaaaa gattttaaag aggtgagaaa atgattggcg aagataaatt
     9301 attgatgact atccagctca cagacttaaa tgaagaagaa aaaaaacagg aaataagcga
     9361 ttgggctatt ttgacacgtc tgttgatcca gccaacattt atccgctctt tcacacaaca
     9421 agcggatccg tatgatcttc gaaagcttca agaaaaaata atgctagcat cggtaagaga
     9481 cgaaagttgg ttagttgtag gaaatgacga gaacgaatgt agttttcgct tggaagataa
     9541 tcaattattg ataaaaaatg tattgtctat ctctgttttc aaagaaaagc aatttttgat
     9601 tcgagacttc attcagaaaa aaatgattgc tcatggggtg ttcgcctata tgcgagctta
     9661 tagcgagttt atctatcaca atacaaaaca gatctctcaa cggctattgt ttgagaaaaa
     9721 ggatgaaatc gaacatttac ctaaaatgaa acaacaaaac ggggaagtag tagtcgattg
     9781 caaccaattt ccaggatacg atctttttta ccaaggttta tgtttcactt cgtgttggga
     9841 aatgtattat agtcgctatt atcatcaaat catccctaaa ccgatttttc tcgacatcca
     9901 acaagtagaa aaagtgaagg aattagaaaa cgaagtcata tgtatccagc tttatcgaga
     9961 cccctttaac tggcaaaagc caaacaatca aatgtttcaa tcctatttta gggaccagct
    10021 tgggtttgat catcttgctt gggacaatgg tgtcggttta ttgaaaccgc cattcgttga
    10081 atacatttac actgaccata cgatccaatc tgtccagtat cagaatgcac aaatgcaacc
    10141 tgttcctaaa aagaatgctt cattttttgt aacaaaaagt tatgatattc aacaaggaga
    10201 ttataaagaa aggcgtgttc gcggaacact aaatgctcag gcgtattttc cttgggttga
    10261 cgagaatcgc tcgcgcatga tgtgctataa agttatcgat ccgactgttg ctttggataa
    10321 tggaatcgaa gcttattgtt attatattag ggaatatcta gaagtagagg taacagatga
    10381 aaaatatcaa gagtatcttt tatccttaag aatctatgtt ccttcagaat ccctgtcaga
    10441 attaccgtta aaggaaatca aacatagact ctctgatgtt accttcaaac gaataaagaa
    10501 aaagcggcga agtatccaat tcgatgtcaa gaaaggtgag cagcatctgc gtgtgcaatt
    10561 tttagattac cgagaattag aacaattgat tactttacaa aaaatatgag gtgaagaaaa
    10621 tggataaaca actctattat cgaataatgg ttccggcatc attattatcc gtctgtctct
    10681 tttttgttcc taattttcct attcgatcag tcatcggttt tttggtcatg tttggcggga
    10741 tgggacttta ttattatctt cttcataagg aaagaaaacc tgcttctgtt tatatgaaaa
    10801 aaacaagaag cgaaaataaa aaacgataaa acggcttgat gatagacaaa aaaataaaaa
    10861 a
//
LOCUS       DAEGIQ010000088        10793 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000088 DAEGIQ010000000
VERSION     DAEGIQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.090"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            352..621
                     /gene="rpsN"
                     /locus_tag="KMT45_002103"
     CDS             352..621
                     /gene="rpsN"
                     /locus_tag="KMT45_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBE7005557.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            636..815
                     /gene="rpmF"
                     /locus_tag="KMT45_002104"
     CDS             636..815
                     /gene="rpmF"
                     /locus_tag="KMT45_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBE7005558.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            847..1224
                     /locus_tag="KMT45_002105"
     CDS             847..1224
                     /locus_tag="KMT45_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005559.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1245..1394
                     /gene="rpmG"
                     /locus_tag="KMT45_002106"
     CDS             1245..1394
                     /gene="rpmG"
                     /locus_tag="KMT45_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBE7005560.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1418..2401
                     /locus_tag="KMT45_002107"
     CDS             1418..2401
                     /locus_tag="KMT45_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBE7005561.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2431..2553
                     /locus_tag="KMT45_002108"
     CDS             2431..2553
                     /locus_tag="KMT45_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBE7005562.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2571..4094
                     /locus_tag="KMT45_002109"
     CDS             2571..4094
                     /locus_tag="KMT45_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBE7005563.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4118..4357
                     /locus_tag="KMT45_002110"
     CDS             4118..4357
                     /locus_tag="KMT45_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005564.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4550..5011)
                     /locus_tag="KMT45_002111"
     CDS             complement(4550..5011)
                     /locus_tag="KMT45_002111"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBE7005565.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5206..5424)
                     /locus_tag="KMT45_002112"
     CDS             complement(5206..5424)
                     /locus_tag="KMT45_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005566.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5542..7158
                     /locus_tag="KMT45_002113"
     CDS             5542..7158
                     /locus_tag="KMT45_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBE7005567.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7399..8739)
                     /locus_tag="KMT45_002114"
                     /pseudo
     CDS             complement(7399..8739)
                     /locus_tag="KMT45_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9015..9170)
                     /locus_tag="KMT45_002115"
     CDS             complement(9015..9170)
                     /locus_tag="KMT45_002115"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBE7005568.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9466..9798)
                     /locus_tag="KMT45_002116"
     CDS             complement(9466..9798)
                     /locus_tag="KMT45_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7005569.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10312..10503)
                     /locus_tag="KMT45_002117"
     CDS             complement(10312..10503)
                     /locus_tag="KMT45_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005570.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10597..>10793)
                     /locus_tag="KMT45_002118"
     CDS             complement(10597..>10793)
                     /locus_tag="KMT45_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBE7005571.1"
                     /translation="TLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIGKVLGIT
                     RQGATNLKHRLYKKIQKMLV"
BASE COUNT         3589 a         1710 c         2096 g         3398 t
ORIGIN      
        1 aaaaattggc gataaaccag ttaaacataa aagattgaat aaaaagttat agtttctttc
       61 atattgccgt tttaagtaga ggaaaagcta cttaaaacgg tatttgtgtt tgattggact
      121 aataaaagtg aattcttatt tttcagagcc taaactgcat attcttttaa atgatttgat
      181 tatctctatg taccgataaa ttcgttttca aatagttatc tattcatatg agttttattt
      241 catgttattt attgttgaca agagcaactc ttgttggtaa agtatcagtg tcaaaacgta
      301 acgattacga tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta tatggcaaaa
      361 aagtcgaaaa ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta tgcagataaa
      421 cgtcaggaat taaaagccgc aggagatcgc accgctctcg ctaaattgcc aagagattca
      481 aatccgaatc gtctgaggct tcgtgatcag acggatggtc gtccaagagg gtatatgcga
      541 aagttcggta tgtcccgtat taagtttaga gaacttgccc atcaagggtt gattcctgga
      601 gtaaaaaaag caagttggta aaaggaggaa atatcatggc agtaccagca agaaaaacat
      661 ctaaggcgaa gaaacggtta agaagaactc atcaaactgt agtaaaacca gaaatttcat
      721 ttgacgaagc gaatggtgat tatagacgta gccatcatgt atctttaaaa gggtactata
      781 atggaaagaa agtaattaaa ggagcttcaa aatagtttaa tatataactt ttaggaggga
      841 atcaaaatgg aaattattta cccaccttta gttgaagaag gattaaagta ttatcttgaa
      901 acgacccagc aatcgctaga taaatgcact ttttatcgtt cgatggtaga gagaggaatt
      961 attactgaaa cgggtttacc gacccaacag gcaattgaaa atggattagt caaagattac
     1021 tatgaggacc aaggcttatc atttgatgat tttttaagaa tctatccaat ttttgaagag
     1081 tatgacgagg agttatttca atgtatcgat ggttattggg agatacctgt tgatatgaaa
     1141 gaaaatttag tttcacaatt ggaatctggc gagttaactt ttgaagatgc acaacaaatt
     1201 caagcatatc ttgaagatcg ataagtattg aaggaggaag atttatgcgt caaaatatta
     1261 ttttggaatg tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat aaacggaata
     1321 accctgaacg gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg gcaattttta
     1381 aagaagtgaa atagctaagt ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt
     1441 ttctgggttt ttaggggctg gaaaaacaac gctaatcaat caagccttgc aagtctcacc
     1501 ctaccccagg gaagaaatta ttataattga aaatgagttt ggtgaagttg ggattgatca
     1561 caaattatta gttcatagcg aggaacgggt gttacaacta aataatggat gtatgtgttg
     1621 cagtcttcgc agtgatttat tagcaatatt gatttcttta ctggaaacaa tagaagaaca
     1681 tcaacaacca atttcacaaa tcatcattga aactacaggt atagcagatc cccaaccaat
     1741 cattcaaaca cttttaacag cgcctcaact aagaggaaga gtatatatcg atagtttatt
     1801 gactgttatt gatagtgaca gttttgaact tattgaaaga gaaccacaag cccttaagca
     1861 acttgtcatg gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt tatctgcact
     1921 aaaaaaatta cagagagaaa tcaaaagtat taacccttta tccgatattc gaatgttcac
     1981 acatagtgaa acgattcaaa aaaaggattt ttataatttg aaaaaattta atcatccctt
     2041 agtttttgaa agtgattcat cagaaaatca ctataatggc aaatactatc atcaacacca
     2101 tcaagtacat gagtttaaat caattctctt aacttcagat caagacataa aaaaagattt
     2161 tttactccaa tggattgact ggctgatttt aaccaaccaa gggagcctgt atcgttttaa
     2221 aggattgatt gctttacaag agcaggattt tatagttgcc atgcaagagg taaacgaaca
     2281 ggttaatttt caatacacca ctcgacaaaa agaccaaacc actattattc taatcggaaa
     2341 aatgttggat gaagaaagga tcaaacagag ttttgataaa ttactggcgg aaagcaaata
     2401 atgataagta gtgattgggg gtaatttcta atggagaaaa cggatatatc tagtgcatat
     2461 cgacgactta aaagcccgaa tattaaaact agaaaaaggg cattaaaaat tatcaaagaa
     2521 cataaacgaa ataaaatgaa gaaattagca taattaagga aggaaaaaga atgaagaaaa
     2581 tcagtgtagg acttattggg atagcagctc taggattatt gggagcttgc tccagcacta
     2641 atgatgcaaa agtatcgaat gataaagatg gaaaattaga aattgtaaca acattctatc
     2701 caatgtatga tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc
     2761 cagcgggatc agaacctcat gactatgagc cctcagctaa ggatatggca aaagctcatg
     2821 atgctgatgt ttttgtatat cataacgaaa acatggaatc atgggtacca aaagctaaag
     2881 aaagttggaa gaaagctggg cctaatgtag tagaaggaac aaaagacatg attttattgc
     2941 caggtagtga agaagaagat catgatcacg gagaagaaga tcaccatcat gaactagatc
     3001 ctcatacgtg ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt
     3061 tagttaaatt gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa aagtatttga
     3121 ctaaactgaa acggttggat gctgactata ccactagtct taaagaagcg aaacaaaaaa
     3181 gttttgtgac gcaacacgca gcctttggat atttagcttt ggattatgga ttgaaacaag
     3241 ttcctattgc tggactaagt cctgaagaag agccatcttc tggtcgatta gctgaattaa
     3301 aagagtatgt taaaaaaaat aagatcaatt atatttactt tgaaaagaat gctaatgata
     3361 aaattgcaaa aactttggcc aacgaagctg ggataaaatt ggaagtactg aatcctcttg
     3421 aaagtcttac aaaagaacaa atggataatg gtgaagatta tgtttcagta atggaagaca
     3481 atctaaaagc gttagaaaaa acaactatgg ttgctggtga agaggtagtt ccagaaaaag
     3541 aagcgaagga tgaaaagaca gttgctaatg ggtactttaa agatgccgat gtgaaggatc
     3601 gcgaattgag tgattataca ggcgaatggc aatctgttta tccgttgtta aaagatggaa
     3661 ttttggatga agttttcgat tacaaagcaa aaatcaataa agacatgaca gctgccgagt
     3721 acaaagatta ttatactact ggctataaga cagatattga tactatcaac attaaagaca
     3781 acaccattga ttttgtagtc aacggtgaac atcatcaata cacatacaaa tatgttggat
     3841 acaaaatttt gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt gaaactgatg
     3901 atgcaggtgc tggtcgtttt aaatatattc aatttagtga tcatggaatt gcaccaagta
     3961 aggcagagca tttccatatt ttctttggtg gagaaagtca agaaaaactc tataatgaat
     4021 tgcataattg gcctactttc tatccagctt cattgtctaa acatgaaatt gctcaagaaa
     4081 tgatggctca ttaaaattca atttttggag gaaatacatg gtaagaaaag tattattgtc
     4141 tatggattcc aagaatattg atgcttatcg gcgttatcga tttgcaaagc aaatgagatt
     4201 aaatgggagt acattagctg atattgctgc aactttacat gttagtattg acacagttca
     4261 tagagtatta aaacgggatc ctgattgttg ttttatcaag gtcaatactg gaagaagtag
     4321 acgtttagca aaacggcata atagcttggc caattaattg ataataccag agtagatcat
     4381 ttttccctta tttggatttg atctgctttt tattatgtct tgaattgtca aatcaagcgc
     4441 aacactttat caaagaagac ttcataaggt gttctccaat ctaaacactt tctaggtctg
     4501 ttattcagtt tttcaatcat ctgatttatc gttgagttat ctaccgtagc tatctctttc
     4561 gattttggca agtattctcg aatgagtcta ttcgtatttt aatttgttca ttgttgccaa
     4621 gggcatgagg atctggaaaa taaaagggca tattattcaa tgcttctgta actgcgaaat
     4681 gattggagaa ttcttttcct ctatctggag tgatcgtttt gaccgcctca tttggaatac
     4741 tgcttaaaag ttcaatcatt ttctcgctca cataagcggc tttctttctg gggaccttct
     4801 ttcctgctaa aaggaagcat gattttctgt ctgtcaaagt cactaaacaa gtaccaccag
     4861 ttttgccagc tacaatatcg gctttccaat gaccaaaatc acttctatca tctgcggatt
     4921 ttggacggcc atggatcgaa tgagtaaggc aaatctttcc acgagactcg acatgatttt
     4981 ttgtatgtct tgtttttcct cgatggtgca atgaacggat caggccttta ttgccgtgtg
     5041 agaggggttc tgtatcgaaa agatcattgc aaattgcacg ttagatcgtg ataaaactaa
     5101 ttgaaaagtc catatgttca tgcttcaaac gattcgagat ttcctctggc gaccactgaa
     5161 aatcgacaaa taaatgacga acaattgacc aaactcacgg atcatttaga atgtgataac
     5221 gtccgcattt ttttcttcga taagcataat ttttttccgc tttggaaggt gaatagtttt
     5281 tttcttgttt cttctcagtt catgtaaaat agtaaacggg ctatgctgaa tgtcgttgcc
     5341 aattatacta atagaaaatc cttcttcgtg aagcagaaag attctttctc gttcgagtat
     5401 agtaagatga cgatactggc tcataattat tttccttcta ggagtgttct cgacaaactt
     5461 atactagttg gatatcgatt atgactcatt tttatgtgtt gcacttaaag tgtaaattca
     5521 aggtatagaa agaggttccc tatgaaaaaa atacctatta caatcttaac tggctatctt
     5581 ggttcaggaa aaacgatact tctaaatcat attctgtacg gtgatcacgg aaaggaaatt
     5641 gctgtcattg taaatgacat aggggaagta aatgttgatg gggtattagt cgacaagaaa
     5701 ggttttaaaa gaactggaga aaaacttgtt tcgatgccga atggatgcat ttgttgtaca
     5761 ttacgcgaag atttgattgt tgaactagat caattagtgc aattttctga cttgaatcaa
     5821 attgttattg aagcttcagg aatcagcaaa ccaattccaa ttattcaagc aattatatta
     5881 gctaaaaacc ctttagacat tgatttgacc caacgattag agattggtgc cattattata
     5941 gtagtggatg taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat tcaggaaaga
     6001 aaagctgaag gtaatgtagt tgaagaagaa caggatattc gtggcttgtt aattgatcaa
     6061 ttcgagtgtt gtaacatatt acttttaaat aatagtgact tagtgtcaac caagacgatt
     6121 tacaaaatta acggtctaat tgctaagtta cagtccaatg ccaaaataat taggacaatt
     6181 aaaggtgaag tagacttatc gaaaattcta aatacaaatc tttttaatat agatgagact
     6241 tcctattcag tgggctggtt caaagagtta gagcttggct atcaaaatca tattcctgaa
     6301 acagaagaat atggtatctc aagttttatc tatcgaaatc ggttactcct tgacgcacaa
     6361 aaatttgccc atcttacgga taaaaatttt cctggcaata ttacaagagc aaaagggttt
     6421 ctttggtttg cccaacatag tgatttttcg acgatctttg aaacatcggg tagctataaa
     6481 agtattgaat cattaaatta ttgggtagct agtttaccta aaattgagtg tcaaatgatt
     6541 atacaaaaaa atcccgaatt gaaggaagcc tggcatccgt tgtttggtga acgcattaat
     6601 aaactagttt ttattggtat gaagatggat caacaatgta ttaaggaaca acttgagggt
     6661 tgtttaactg attctcttaa tgcagataat ttccgaaaaa tttcaccatt tccatggaaa
     6721 aagtctaata tgtttagcca gatatcgtta atgaaatttt taagaaaagc tggtgtcaag
     6781 ataggcgatg tcataattat tggcattttg attattacct cttttattcc cttatttgta
     6841 ttctctatag aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc catcgttcga
     6901 attgatggca aggaaatgga tcgcttcgat ttggataaga tcaatcataa actcgtgaat
     6961 tatcatcctg cgaagggaca atataatatt gttgagatta aagatggaag aattcgagtt
     7021 aaagaagata atagtcctga tcaaatcgcg gttagaactg gatggatctc aaaacccggt
     7081 caaacgagta tttgtttacc ccataagtta gttattagta ttgagaaaaa agaaagcaca
     7141 gattactaca tttactaatc tggaaataga tgaggaggtt tttaataaga aaaagatatt
     7201 attattaata atagcagggg ctttattaag tgtgttaagc gcttgtggac aagaaaaaga
     7261 agcggtgaca acaggcacaa gtatctttaa cgggacctgc aatggtttag ttaacagtcg
     7321 gttagattac ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata
     7381 atcgagatac tcacacggct agacagctat cttgtctagc cgtgttgttg ttctggcttt
     7441 agtcagttgg gtagtattct tagttttctt aaaatatttt cctcgatata aaaagattcg
     7501 ttctcggtag ttttgaaagt ttctatagcc ataggcaatt cgttttagta ctttgatatg
     7561 gttattcaaa cattctatag ggccgttgga atagggaacg cgcaacgctc gtttgatttc
     7621 tgtttggtat ttcttgaagg tcgtgaacac tggttggtag tgctctggca actccttgta
     7681 gtcttctttt aggagcgcga cgaattcagg aacatcttgt cttctgataa cggaaagaaa
     7741 atcctggtag acttcgtagc ctcgctttaa ggaatcatca taagataata gccgatcgac
     7801 tatctctgct tctgtcagat gatctcgaaa tgacggacgc cagtagcgat gctgaccttt
     7861 caattcccac gcattctttt gaagtagttt ccagtatttc tttaagtgct tcgaccgacg
     7921 atcccctttt ttaaaggtct tcattacttg aattcggtgg ttggtaaaag cccgaccaat
     7981 gtgttggcta atatgaaagc gatcggcgac gattcgggca ttcggaaaac actctttcac
     8041 caatgtgcga tagggtgtat agaaatctga aaccactaga cgcacttgtt cacggacttt
     8101 tatagggtag cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt ccacaatatc
     8161 aatcagttga tgggtcttgc cgtccatcat gacaaagctc atggctcccg agacattttt
     8221 cactgatttg aattcatcga aacacaccac ttctggtaac agctggctca aagaaatggt
     8281 tttaggccga aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt gcttcatacg
     8341 tgcgatcaaa gacatagaaa gaggttcgct taatagctca agaatggctt gtttgactct
     8401 gcgagcaatg gagcagcgac gatcactaac tgaatcttct gctaaaaaag tctttccaca
     8461 gtgtttacat ttgaagcgct gtttgttaat ccgcagaatg gtcttgtagt tactgacatc
     8521 attcaataag atggatactt ttttccagcc ctatttaatg atcgtttgtt cattttgata
     8581 gtggcgatga aagcattcct ccggttgata ggtcaatgtg ccatcaaaaa caaaacatgt
     8641 ttcgtcattg attcgctctt tgcgaaaggc atcttcatga aagataagat ttaggtctag
     8701 aatatctagt aactctttgg taaactctga ataggacatc ttgaaacact cctttgaatg
     8761 gttttcgtcg actttattct aaaggatttt aaggtgtctg tttgcgttta ttagaaaaaa
     8821 gaaaaagtat tggcaacaga aggcgtcacc aacactagat attatagatc ccttattttc
     8881 ttcgcttcaa attgtcaagg agtcatcata aattttgtat ggattctttt gtttgggtag
     8941 tagtcttcta aataatcttg gattaacacc ttcatctcta ttttactttt atatttgttg
     9001 gagtcgatta attctcattt gattgatcga tgaaacgctt caattactgc attgtcaaca
     9061 ggagtcccag tttggctcac tgaatgtgtg atgtggtggt cgttcaacag ctcttgataa
     9121 ataaaagacc gatgctggct acctctatcg gaatgaatta ccgtaggcaa ttctccttaa
     9181 taaatttgta atggacggac gacaatctct tttttcatgt gtgaatctac catatagtaa
     9241 cgtagaatac gagttgctaa gtcaatatag gtggacaaat agaggcgacc gtcattacat
     9301 ggaatgtaag taatatccat cacccaaaca gcatcaattt ggttctgttc aaattatcga
     9361 ttcaacattt ttttaccatt tctttgattg ttttttctct accatgagaa gatttgcgtc
     9421 gatgaaatcc tcttgtgcat aaattcattt cacgcataag aacagctaca acacgtttat
     9481 tgatttcgat tccttcatcg taaagttttt gagtgatccc tggagatccg tatcgatctt
     9541 tgtgttcgta gaacactcgt cttcctcttt tagtcgttta ttttcctgtt caagaatgta
     9601 gaaatctgct ttatcaggta atctttttcc ttgtccagca aaagcatttt tcccgtatcg
     9661 acgatattct gaaacccatc gggaaattgt tggacgagag atattgtatt cttcagcaat
     9721 tttcttggta gttacttttt tctcaaggat atcctgtgaa atctttagtt taaatttacg
     9781 atcatatgtt tttctcaata gagttcattc cttccggtaa cgtaactgat agtcaagaaa
     9841 agtgtatcat agcccaactt agtatcaggg tttgtcaact aaactgtgga agttgataga
     9901 taagagtttt taacaaccaa aattctcttg actatttttg aaatcagaca aattttgatt
     9961 ggacacatag ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg cttttcaatt
    10021 ctagaggaga agtcctcgtt gaagtaccat agcccagttc tggattcgac ctcctgccca
    10081 cttcatacgt aaataaagta attttaggag tgagactgtg acaaaagtta tttttaagct
    10141 aaaaacgaat taaaaaacgt tgataattaa aatcattcgt tttagattct atgaatcatt
    10201 atagaacgct gtaaaatcga tactttataa ttataaagaa gatagtttca aatttattgt
    10261 tcgaaatttg aaactatctt tttacttatg tcacagactc ccttagttga gttattcttt
    10321 aatttcgaat ttcgagatag ctagatatat tgcaagcata cattcttgat aacaatcttg
    10381 atcatatttt ccagttctcc aggattgctt atgaatcaaa gagctaaatc ttttgagaat
    10441 tgcctcaaca gcttcttcat ttccagcctt tgctaaaatg aaatcttctt ttaaagaatt
    10501 cataaatcta ccttcttccc atggacttat aatctatttc tttgggaagg aggagaacga
    10561 gcaaagaagc atacaaaatt agttattatg cagatattaa accagcattt tttgaatttt
    10621 tttgtaaagt cgatgtttta aatttgtcgc accctgtctt gtaatcccca gaacctttcc
    10681 aatttcttta tcagttttat caaagataaa tttttcgata agaaaaattt gttcaggttc
    10741 tttcaacaaa ggaaggattt ttgctaattt tatatcttca acaaggagag tca
//
LOCUS       DAEGIQ010000089        10760 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000089 DAEGIQ010000000
VERSION     DAEGIQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.091"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(59..1042)
                     /gene="istA"
                     /locus_tag="KMT45_002119"
                     /pseudo
     CDS             complement(59..1042)
                     /gene="istA"
                     /locus_tag="KMT45_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="KMT45_002120"
     CDS             complement(1437..2279)
                     /locus_tag="KMT45_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBE7005572.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="KMT45_002121"
     CDS             2604..3020
                     /locus_tag="KMT45_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBE7005573.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="KMT45_002122"
     CDS             3017..3487
                     /locus_tag="KMT45_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7005574.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="KMT45_002123"
     CDS             3501..4274
                     /locus_tag="KMT45_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7005575.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="KMT45_002124"
     CDS             4288..5100
                     /locus_tag="KMT45_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE7005576.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="KMT45_002125"
     CDS             5154..6119
                     /locus_tag="KMT45_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBE7005577.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="KMT45_002126"
     CDS             6256..8979
                     /locus_tag="KMT45_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBE7005578.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="KMT45_002127"
     CDS             8992..9624
                     /locus_tag="KMT45_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBE7005579.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="KMT45_002128"
     CDS             9829..10056
                     /locus_tag="KMT45_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBE7005580.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="KMT45_002129"
     CDS             10125..10391
                     /locus_tag="KMT45_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBE7005581.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="KMT45_002130"
     CDS             10381..10650
                     /locus_tag="KMT45_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE7005582.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3566 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt
//
LOCUS       DAEGIQ010000090        10640 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000090 DAEGIQ010000000
VERSION     DAEGIQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.092"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(80..2200)
                     /locus_tag="KMT45_002131"
     CDS             complement(80..2200)
                     /locus_tag="KMT45_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBE7005583.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2430..3608
                     /locus_tag="KMT45_002132"
     CDS             2430..3608
                     /locus_tag="KMT45_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBE7005584.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3624..4382
                     /gene="aroD"
                     /locus_tag="KMT45_002133"
     CDS             3624..4382
                     /gene="aroD"
                     /locus_tag="KMT45_002133"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBE7005585.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4405..4890
                     /gene="ybaK"
                     /locus_tag="KMT45_002134"
     CDS             4405..4890
                     /gene="ybaK"
                     /locus_tag="KMT45_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBE7005586.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4961..6214)
                     /locus_tag="KMT45_002135"
     CDS             complement(4961..6214)
                     /locus_tag="KMT45_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBE7005587.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6330..7679
                     /gene="gdhA"
                     /locus_tag="KMT45_002136"
     CDS             6330..7679
                     /gene="gdhA"
                     /locus_tag="KMT45_002136"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBE7005588.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7791..9137
                     /locus_tag="KMT45_002137"
     CDS             7791..9137
                     /locus_tag="KMT45_002137"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBE7005589.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9445..9831)
                     /locus_tag="KMT45_002138"
     CDS             complement(9445..9831)
                     /locus_tag="KMT45_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBE7005590.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10099..10248)
                     /locus_tag="KMT45_002139"
     CDS             complement(10099..10248)
                     /locus_tag="KMT45_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005591.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         3426 a         1912 c         2123 g         3179 t
ORIGIN      
        1 attattctaa gatttctctt taccaacatc atttgacaaa gagcctaatt ttgtcgctgc
       61 tccttttaaa tttattttat cattcggaag aacttgtggt cgttccgtga agttcttggt
      121 acgtttgcgt tttataaaga tcaacagttg attgtccctt gtgttcacta aagacactag
      181 ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca ttcttgtagt
      241 cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga agaagatcac
      301 cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc agtgaatcaa
      361 tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg ttactataga
      421 tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg aaagaaacaa
      481 gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt tttgtatcta
      541 cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa gtgtgattga
      601 ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc atttgtgcat
      661 tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga tttttatttc
      721 ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta tcatacaagc
      781 cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa ttggctgtcg
      841 caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct gcttcgtcat
      901 tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa taaaatggtt
      961 gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca tgcaacccgt
     1021 attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa taatcatatc
     1081 ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca tggaataccg
     1141 cactagtgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa gtattttttc
     1201 cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt tccatcagcg
     1261 tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca aaactgaacg
     1321 tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcaagctgg agaccattct
     1381 catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg atataaatca
     1441 cattttttcc tttagcgagc ccgaacaaat catcatttgg tgctgcataa tgttctttta
     1501 cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact tggtttgtct
     1561 ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat ttgaccaaat
     1621 aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc ataaaaagat
     1681 ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt gctcggatcg
     1741 ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata ataaaatcga
     1801 tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc agaccactcg
     1861 ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat tctcgataat
     1921 aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc atcgtgatat
     1981 aagcaactct agggcgtctg atataaagag caattccaag taataacaat gtagttgcaa
     2041 tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga tggaaatcaa
     2101 ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacaac tagtaaagaa aaaaatccga
     2161 gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc ctttctgcac
     2221 ttcttaatca cagaagtgtc atgaaaccat gttacacagc tcacttattc tcatttattt
     2281 tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt cattaaggtt
     2341 ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt tgttatactt
     2401 gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa agagcaagca
     2461 atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat ccccaaaaag
     2521 aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca aaaggatatc
     2581 taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc ccatttgatc
     2641 aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc tttttttcag
     2701 atgattctac taccgctttt cgtatattca atggagaagg agacgggatc ggcggtctga
     2761 ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg ttatatgcac
     2821 atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcgta ggaggctatg
     2881 agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac ggaaaagagg
     2941 caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat ttaaatgaag
     3001 gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta gcagaaggtt
     3061 tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt tctgttgctg
     3121 ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc agtttaaaaa
     3181 aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa atcattgtta
     3241 tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat gacatgattg
     3301 tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta gcaaaagatt
     3361 atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata cttattgcgt
     3421 ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa gaggaattcc
     3481 atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa gatttccaga
     3541 cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa attaaaaata
     3601 accattagaa ggagcgatgc agaatggttt gggtaaaggg attgaatata ggaagcggta
     3661 gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta cgtgaagcag
     3721 ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat tatcgtgaag
     3781 ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg cttgatggac
     3841 tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac atatcgatcc
     3901 gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat atggtcgata
     3961 tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc aagtctttaa
     4021 acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac cctgtcttgc
     4081 tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg gcagtaactc
     4141 ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca aatgcttttg
     4201 tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct cgtctggctg
     4261 gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct acttctgctc
     4321 caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat gaaccgttgt
     4381 agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac gaatgcaatc
     4441 cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg ggacgaaagc
     4501 cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctca tatatttaaa
     4561 acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc gggagaagcc
     4621 gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga aatgctccat
     4681 ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc tataggtatg
     4741 aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca aatcattgtc
     4801 tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg tgcattaacg
     4861 agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag aaggaggccg
     4921 ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt tgcccggcat
     4981 tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg ccagtataac
     5041 cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta ctctccaatg
     5101 ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat ttctcaatag
     5161 gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa tcttgaattt
     5221 gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca agaaagaaaa
     5281 ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt ttggttgctt
     5341 tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg aaacaatcca
     5401 gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa cttgaacaac
     5461 gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc gtatgttcag
     5521 tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa gaaagccctt
     5581 gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct gtcgcataaa
     5641 gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca gtagaaccag
     5701 tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg aactcttcga
     5761 attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct actcgagtag
     5821 agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg ataatcgttt
     5881 ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa tgtacgcctt
     5941 gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta ctatataaaa
     6001 actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg ttcgtaacat
     6061 tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt ttttcgatca
     6121 agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg cttgttccag
     6181 cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga aagtgtagca
     6241 tatctgaaga aagaaaacag aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg
     6301 agggaagata agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa
     6361 ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg
     6421 aattcatgcc cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata
     6481 tcttagaact tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg
     6541 atgaaggaaa ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc
     6601 cttataaagg tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg
     6661 cttttgaaca gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg
     6721 gaagtgactt cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt
     6781 tcatgctgga attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg
     6841 gtgtaggagc tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg
     6901 atactggtgt gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag
     6961 ctactggtta tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat
     7021 ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa
     7081 aagtgcaaga gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg
     7141 atccagaagg aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt
     7201 tgacggaata cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga
     7261 cattgaaaga aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa
     7321 gcttggccga aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc
     7381 catgtacagt aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa
     7441 aagctgctaa tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgcccagc
     7501 gattgacttg ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt
     7561 accatacgtg tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag
     7621 caaatgttgc tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa
     7681 aaatgaaact ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa
     7741 aaaaaacgta aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca
     7801 ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc
     7861 aaagccaagt aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact
     7921 tccgcggctg gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag
     7981 aagcagctaa aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt
     8041 cttatttagg cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag
     8101 atagcaacac aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat
     8161 atattgctga cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa
     8221 aatctggaac aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta
     8281 agaaatatgg acaagaagaa gcaaacaaac gtatctacgc tacaacagat aaagcaaaag
     8341 gtgcagttaa agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca
     8401 tcggcggacg tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg
     8461 cagatatcga tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca
     8521 aactagaaga aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag
     8581 gcaaagtaac tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt
     8641 ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg
     8701 caaacttctc aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata
     8761 tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag
     8821 cagaagattt agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa
     8881 aagcatttga aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca
     8941 aaattccaga aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg
     9001 taggtatttc aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt
     9061 acaagaaaaa catgttcgca cttctaggca aaccgggctt tgaagatctt gctaaagaat
     9121 tgaacgaacg cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac
     9181 atgttcgctt taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt
     9241 attcagaata aggggctgtg acaagacgtt tgtcacagtc ccttgtttgt atagaaatcg
     9301 attgattgcc ttgctcttta gtttgtttta ggactttaag aacagtgagg ctgacttcga
     9361 aggttaattc tgtttttttg ttttctagaa tcagtaagca aaaaagcact cacttcgagt
     9421 gcttttttgc gatatattct tagcttaaga taggcctaat ttttctttta cagatgaggg
     9481 taccttatcg ctggtaatta cttttttctc tatgatccgc ttttcgcttg ctttcaactg
     9541 caagatcgtt ccttcggtgt aagcagaacc attggcagag tcaccagaag taatgacatc
     9601 gaaatctaac tgtctttttt caccgttttc agcataagca tctacgttgt atttaaaacc
     9661 tcttccaatt acttccccat tatctgcttt gatatcagtt tctgcaggca ttggagcttt
     9721 gactaccgca taataatctt tacctacata agtagactga tagtagtccc aacctttcca
     9781 tcctataaaa gcaatcaata tgataacgat caaccctaaa atttttttca tgaaagatct
     9841 ccttttttat ttttattata ttaaatatta tacagataat aacggaatat tatcatcgaa
     9901 acagctaaca aaattgtcat aaaaaacgag atggttgata ttagaacttg tgtttttgtt
     9961 taggtctaga gataaagaga gacaatttcc aaatatatgt aatgaataca aaaagacgtt
    10021 tgtcgcggat taccgttaca aacgtctttt attgattttc cgagatctca tcagtcgcca
    10081 tttggaatgg ttcactcctt atctagattc gtttaatgct cggactgctt gttcgattgt
    10141 aataccaaac gcaacgttat tttcgttttg tgcatcaatc gccataaaac ggttagaagt
    10201 tgtatcaaaa gctacgcggt attgcatatt tgtttttgta aacgtcataa aattcatctc
    10261 ctttgtaatg taatttagtt cttgaaagtt tcctttaaac tttcgaactt ataccaagtt
    10321 tagcatcgta cgttttttga tgcaattaaa aagcatttat cattttcctt atttattttt
    10381 tcttaaatgg tttagctatt ttcttttaat ccgataagta gcccaatcat caaggttaca
    10441 aatactattg cttgaatata aatcatatgc tcacctcttt ataaataact tagcaatata
    10501 aagaaaaaaa cgcacgaaca acataggaat ataaacaaac gtttttgtca caacgtctaa
    10561 gatcgtgtta aaaaatagtt atctaaaaag cagaaagata ctcaaaataa aaaataaaaa
    10621 aatgaataaa aacgttttca
//
LOCUS       DAEGIQ010000091        10550 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000091 DAEGIQ010000000
VERSION     DAEGIQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.093"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..279)
                     /locus_tag="KMT45_002140"
     CDS             complement(<1..279)
                     /locus_tag="KMT45_002140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005592.1"
                     /translation="MRIKGKNISWVFYMLGILAFTFVAIKLSEIIIPNDSVVSKNLVL
                     ERAKLIISILGPALTLIVFVRTIQTQLESSKNLIKDNVSKDFYNLLNLF"
     gene            complement(311..2002)
                     /locus_tag="KMT45_002141"
     CDS             complement(311..2002)
                     /locus_tag="KMT45_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBE7005593.1"
                     /translation="MLFNQHVDQYIRKWKSGELILNEKRIQLLKLIEKEILPRDDIYY
                     FDEEQINNYIEFSQAWYFELDEWEKFISAFIFLFYKEDDEVVFDEFVINMGRGGGKNG
                     FISTLANYFVSSLHGIDYYDVSIVANSEKQAKRSFQECYRVINKKGNEDLKEEFEAYK
                     SSLTGLETQSVFEYKTSNASSQDGGREGAVIYDEYHEMENTDIVDVFSGGLGKVDHGR
                     QFFIGTKGFVREGYFDIKYRECEDILNGLSEFKGVFPYICELDAIEEMDDPKNWPKAN
                     PALQPPLNKRGKRLFNEVMKKYKKLAKEPSGRSAFVTKRMNFLEDNMENSVASREEIM
                     ATNRPFFELDTVPIGSLDFGSVRDFATCGLLFKNRKKSEYAFKSFTFAMKNFCDVHYG
                     YSLKEQLVGSEKRAPIKTWEKMGLMKVIDEPSLNPKYIVDWFIQAREEYGVRVIVVDN
                     YKADILGPMLEKEGFEVVRLKRPSSLHPLLAPRIEDGFANHKFIFGDNPLMRWFTNNV
                     YVKETQAGKQFLKKEEVKRKTDGFQAFVHALYKATELDEQIDYDDAFDMLDEIEF"
     gene            complement(2005..2559)
                     /locus_tag="KMT45_002142"
     CDS             complement(2005..2559)
                     /locus_tag="KMT45_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005594.1"
                     /translation="MIGLNSKLVGTRMKQKRIEKGLTQADVATKSGISSRYYGSIENG
                     KNSPSIETLNSISIVIGCSLHFLLNDRMDDMEKRVELEKKRLQKIFDKIPHDKLNLVE
                     GLIIQAARLRVLLDDNWKDIIENGEYEKFKQSENQIAYDRKRPIVENYDNRDKTYQAI
                     VKQLTDLLPQGTRADKKSKLLGRK"
     gene            complement(2727..2987)
                     /locus_tag="KMT45_002143"
     CDS             complement(2727..2987)
                     /locus_tag="KMT45_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005595.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEETTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            complement(2978..3289)
                     /locus_tag="KMT45_002144"
     CDS             complement(2978..3289)
                     /locus_tag="KMT45_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE7005596.1"
                     /translation="MIDVSTKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            complement(3290..3862)
                     /locus_tag="KMT45_002145"
     CDS             complement(3290..3862)
                     /locus_tag="KMT45_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005597.1"
                     /translation="MKLKDYIREGYNIVTTPNLAYKIQEDYPNVLVFTDRALDAIPIG
                     KLLVDRYYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWHTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            complement(3903..4187)
                     /locus_tag="KMT45_002146"
     CDS             complement(3903..4187)
                     /locus_tag="KMT45_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005598.1"
                     /translation="MNDNYDYIKLIEKIRAEKDMDELANLFMNIISLVGLKMDEVAAL
                     NYFIAEQTIRAEHNAKFLKDRLDLDVKGLGVEGIFKVQEALVNVYVEKMQ"
     gene            complement(4184..4411)
                     /locus_tag="KMT45_002147"
     CDS             complement(4184..4411)
                     /locus_tag="KMT45_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005599.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIIEMTREAT
                     PNEIDKYNLRYCGHGYWKDEYIQQNIERYIK"
     gene            complement(4418..4630)
                     /locus_tag="KMT45_002148"
     CDS             complement(4418..4630)
                     /locus_tag="KMT45_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005600.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQSKYFYDQL
                     ILQGFNEGQAMEFMMRTFSASNQQKE"
     gene            5177..5530
                     /locus_tag="KMT45_002149"
     CDS             5177..5530
                     /locus_tag="KMT45_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005601.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
     gene            complement(5930..6397)
                     /locus_tag="KMT45_002150"
     CDS             complement(5930..6397)
                     /locus_tag="KMT45_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBE7005602.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(6472..6912)
                     /locus_tag="KMT45_002151"
     CDS             complement(6472..6912)
                     /locus_tag="KMT45_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE7005603.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(6942..7235)
                     /locus_tag="KMT45_002152"
     CDS             complement(6942..7235)
                     /locus_tag="KMT45_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005604.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            complement(7232..7435)
                     /locus_tag="KMT45_002153"
     CDS             complement(7232..7435)
                     /locus_tag="KMT45_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBE7005605.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            complement(7442..7672)
                     /locus_tag="KMT45_002154"
     CDS             complement(7442..7672)
                     /locus_tag="KMT45_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005606.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            complement(7669..8007)
                     /locus_tag="KMT45_002155"
     CDS             complement(7669..8007)
                     /locus_tag="KMT45_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005607.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            complement(8004..8402)
                     /locus_tag="KMT45_002156"
     CDS             complement(8004..8402)
                     /locus_tag="KMT45_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBE7005608.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            complement(8399..8617)
                     /locus_tag="KMT45_002157"
     CDS             complement(8399..8617)
                     /locus_tag="KMT45_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005609.1"
                     /translation="MDLYDIESGATVSVESDHLIISFADERQIIIWVVDDMLYPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            complement(8604..8960)
                     /locus_tag="KMT45_002158"
     CDS             complement(8604..8960)
                     /locus_tag="KMT45_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBE7005610.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            complement(9099..9914)
                     /locus_tag="KMT45_002159"
     CDS             complement(9099..9914)
                     /locus_tag="KMT45_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBE7005611.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            complement(9925..10143)
                     /locus_tag="KMT45_002160"
     CDS             complement(9925..10143)
                     /locus_tag="KMT45_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HBE7005612.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            complement(10140..10451)
                     /locus_tag="KMT45_002161"
     CDS             complement(10140..10451)
                     /locus_tag="KMT45_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBE7005613.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
BASE COUNT         2931 a         2272 c         1551 g         3796 t
ORIGIN      
        1 gaataaattt agcaaattat aaaaatcttt actgacgtta tctttaatta agttttttga
       61 agattctaat tgagtttgaa ttgttcgaac aaaaacaatt agtgttaatg ctggtcctaa
      121 aattgaaata attagtttag ctctttctaa aaccaaattc tttgaaacta cactgtcatt
      181 tggaataata atttctgata gttttattgc cacaaatgta aaagctaaaa tccccaacat
      241 gtaaaatacc caagatatgt tttttccttt tatcctcatg aaagcacccc tttgtaaaga
      301 aaagtatacc ttaaaattca atctcatcca acatatcaaa agcatcatcg taatcaatct
      361 gttcatctaa ttctgtagct ttgtacaatg cgtgtacaaa tgcttggaac ccatcagttt
      421 tacgcttgac ttcctctttt tttaagaatt gtttgcctgc ttgcgtttcc tttacgtaca
      481 cattattagt aaaccaacgc attaacggat tatctccaaa aataaatttg tgatttgcaa
      541 atccatcttc tatacgagga gctaataatg gatgtaaact agatggtctt tttagtcgaa
      601 ctacttcaaa tccttctttt tcaagcatag gccctaaaat atctgcctta tagttatcca
      661 cgacaattac tctcactcca tattcttctc tagcttgaat aaaccaatca acaatatact
      721 taggatttaa agatggctcg tcaataactt tcatcagccc cattttttcc caagttttta
      781 ttggtgcccg tttttcactg ccaaccaatt gctctttcaa cgagtatcca taatgaacgt
      841 cgcaaaaatt tttcattgca aacgtaaatg atttaaacgc gtattccgat ttctttcgat
      901 ttttaaatag caaaccgcaa gtagcaaaat ctcgaacact accgaaatcg agagatccaa
      961 tcggtaccgt atctagttca aaaaatggac gatttgttgc cattatttct tctcttgaag
     1021 caacagagtt ttccatattg tcttcaagaa aattcattcg tttagtaaca aatgccgagc
     1081 gtccagaagg ttctttagct aactttttgt actttttcat gacttcgtta aacagacgtt
     1141 tcccacgttt attcaacggt ggttgcagtg ccgggtttgc tttcggccaa tttttggggt
     1201 catccatttc ttcaatggca tccagttcgc atatgtacgg aaatacaccc ttgaactctg
     1261 acaaaccatt taaaatatct tcacactctc tatatttaat atcaaagtaa ccttcacgaa
     1321 caaacccctt agtaccaatg aaaaattgcc gtccgtgatc aactttcccc agtccaccag
     1381 aaaacacatc tacaatatct gtgttttcca tttcgtggta ttcgtcgtat attactgcac
     1441 cttctcgacc gccatcttga gagcttgcat tactagtttt gtactcaaaa acagactgtg
     1501 tctctaaacc ggttagactg cttttatacg cttcaaactc ttcttttaaa tcttcatttc
     1561 cttttttatt gattactcga tagcattctt gaaaacttct ttttgcttgt ttctcactat
     1621 tagctacaat tgaaacatca tagtaatcaa tcccgtgaag cgaactgaca aaataatttg
     1681 ccaatgttga aataaagcca tttttacccc cgccacgccc catattaata acaaattcat
     1741 caaaaacaac ttcgtcatct tctttataaa acaaaaaaat gaatgcagaa ataaatttct
     1801 cccattcatc taactcaaaa taccatgctt gactaaactc gatataatta tttatttgct
     1861 cctcgtcgaa gtaataaatg tcgtcgcgag gtaatatttc tttttctatt agttttaaaa
     1921 gttgtatcct tttttcgtta aggattaact caccagattt ccatttacga atatattgat
     1981 caacgtgctg gttaaaaagc atttttattt cctaccgagt aacttacttt tcttgtccgc
     2041 tcttgtgcct tgcggtaata aatcggtgag ttgtttgacg atggcttgat aagttttatc
     2101 tcgattatca taattttcaa ctataggacg ctttctatca taagcaattt ggttctcgct
     2161 ttgtttaaat ttctcgtact ccccgttttc aatgatgtcc ttccagttat catccaacaa
     2221 gacacgtaac cttgctgcct gaatgatcag cccttcaact aaattaagtt tgtcatgagg
     2281 aattttatcg aatatcttct gcagacgttt cttttctagc tctacgcgtt tttccatgtc
     2341 gtccatgcga tcattgagta agaaatgtaa actgcatcca atgacgatgg aaattgagtt
     2401 taacgtttcg atactagggg aatttttccc attttcaata cttccataat acctactaga
     2461 aataccactt ttcgttgcta catcagcctg tgtcaatcct ttttcaattc ttttttgttt
     2521 cattctcgtg ccaaccaact tagaattcaa accaatcacc ttccttctat atttactaaa
     2581 ggggcggcgg aggggggagg ggtaaaataa atacgttgca tttgcagaat taaccccatc
     2641 caccggttcc caaaaatttt atttcttctc aaaatctttt gaccgggggt atttcactct
     2701 atatatcttc gctttatact gatttatcag ccgtcttgct cgttgctttc tacaatatgt
     2761 gcaaatggac tcaaggtata atcaatgtct acattaatat taaatgtccc tgttacttca
     2821 ttagcgttac ctattagtct attgttgata gatatatcct ctacagttgt tgtttcttca
     2881 atgagctgac tcttaaatag aaccaactta tcaatagcct tatcaaccat agcgattgct
     2941 tctgctcgtt tgacatcatc tctgtttgct gtttttctta ccaccattca tcatcccact
     3001 ttcttttctt ttgttggcca cgatatttca tacggttgtg ccttttgtta tggcaatctt
     3061 tgcataaggt tcttaggttg tttatatcta gagctaaatc cggatgatac tccagctcct
     3121 ttatatgatc cacttcaaga attgaatcat gttgagttgt tagtctaccc tctgctttac
     3181 accattggca ctcataatga tctcgctcta agcattgact tctaagtttc ctccactcag
     3241 ttgatccata gaacctagct cttgcttgtt tagttgaaac atcaatcatt tacatatctc
     3301 tcttgtgccc aatgattttt ctatctaagt acttgttatt atgtgaatca taatattcaa
     3361 tagtgatgtc gttagctcct tttggtactt ggcattcata ttcttccgta tgccaatgat
     3421 aagtaatatc gacaagacct atcggcatct catccattcg ttgaccttta taataaactt
     3481 ctggtactga gtcagtatct cttagtttga tttctagaag gttcgtatta cttccatcat
     3541 ctaacgtcaa ttcagcaatc ctctttttga tctgttgaat tacttttctt cctgtctcgc
     3601 ctgtcattgg tactaccgtc tcatatccag taaactcgca agtaaagcgt tcaatagcat
     3661 aagcgttttg aagtggctta gcactaagaa cagctggatt gttgctataa taacgatcta
     3721 ctaataactt ccctatagga atggcatcta aagctctatc agtaaacact aaaacattgg
     3781 gataatcttc ttgaattttg taagctagat ttggtgtagt tacaatgtta tacccttccc
     3841 tgatataatc ttttaatttc atagtttccc tcctgaaaac atccaataat tacttgttaa
     3901 tgctattgca tcttttcgac ataaacattc actaaggctt cttgcacttt gaatatccct
     3961 tcaacgccta atccttttac atctagatct aacctatctt tcaagaactt agcattgtgc
     4021 tctgctctaa ttgtttgctc agcgatgaaa taatttagtg ccgctacttc atccatcttt
     4081 aaccccacca aactaatgat gttcatgaat aagttagcta gttcgtccat atctttttct
     4141 gctcttatct tttcaatcaa cttgatgtaa tcatagttat cattcatttg atgtacctct
     4201 caatgttttg ttgaatatat tcgtctttcc aatatccatg gccacagtaa cgaagattgt
     4261 acttatcgat ctcatttggt gtagcttctc tggtcatttc aatgatggag tacttctttt
     4321 tgatttgtac agaacgcaca acacgcactg aacaatcatc aatggttcga ggatattcat
     4381 tagttagcga tatataccag tagtttctca ttatgtatca ctctttctgt tggttactgg
     4441 cagaaaaggt tcgcatcata aattccatag cctgtccttc attaaatcct tgcagaataa
     4501 gctgatcgta gaaatacttt gattgttttg cgattaacgc taagcttttt tgagtttcgt
     4561 agaatgtcgc ctccattgtt ttgtttgctt cattcgtttt aaacaactct cttaattggt
     4621 tttcgttcat ttgtttactc ccctttcaaa ataaaaagat cactcgatga gtgatctaat
     4681 atgtaatagc aacctacacg atgcacaaaa cgcgtacgaa attgcgcacc cctatatttt
     4741 taaaccgccg attcttcggt tgctaacgaa tactgaaaca gaataccgtc tctgtccatg
     4801 accaacgcga cagtacaaag ctttgtcgca agcaggttat cgtattccta ataaccccaa
     4861 ttaatgtttc tctcctaacc tacacctgag agtggcgcac gtctgcgcta gtattttatg
     4921 ccttttaact gctagtggca tcccgactag tcagccaatt catcggcatc cgataagatg
     4981 aattgtttcc gtaggtttct taagtcactg gcaatgaatc gaacattgca tggttgccga
     5041 agcattgacc tagcacgcat gcttagcgtc tacctttccg ccacagtgac aaattaatat
     5101 tgtgaaaata aatactaagc gtataatttt agttatcagc gagtggtccg ctgaaataat
     5161 ttaaggtggt aaaaaaatgg caaaaattga tgattaccgt tcagacatga ttgaatatat
     5221 tgaactcttc gctaaaaatt ctaacaataa tattattatt caaactggag gagcaacaat
     5281 ttgcggaact cctatagact ttgatgctga agtaaaagta aatcctctta ttgatgctat
     5341 gatggattct tttgcagaat ttcgctcaaa aaaaattgat gatatagaaa aagatgatga
     5401 gcaaagtttg gtagtcaaat ctatctttct gaaagatgtt accatcatag gcgagagaac
     5461 tactaatatc ccatttttag ttgtttttgc tgatcagatt tctgcaattt ctctcgggaa
     5521 cttggagtaa caatagattc atttaaatta tctatattga ttaaattgct aatctctcgg
     5581 ctattacaaa tagctgagag gatttctttt atttctcctg aagtaccttc aatcgataat
     5641 ttcatattct taccctccaa cacataattt aatagacagc agcatacgaa gaattcagaa
     5701 ggagttgagt tcacatcctt attcttaata tttccgctgc tgtctatcga ggcttaattt
     5761 aaaacgatga gggagatttc ctcccttaca ttttattttg tctaagacct atcactaatc
     5821 tttcgacact atcataatac aacgttgaat aggtaagtga ttggtataaa aaaggtataa
     5881 aatggaaacc aaattggtaa taaaagggta taaaaagtgt aaaaactggc tacttgaaag
     5941 caaccagttc taacgatgaa gcaaattgga tgataattct gtttgattct actttaaccg
     6001 attcttcgct agtattattc ctttgagcag ttacataaat gggcagacca ttgatataac
     6061 gatcatagaa tatcttcttg cgcctttcag tcacatcagg cttatgcgga tgctgtatcg
     6121 ctgaatagcc tcgaacaaac aatttatgca gatactcaaa ttcttcctgt gcttcttctt
     6181 tatcgatcag cattctttct gcttcaaata tatgatcagc tgtagaaggt ggaaccaagg
     6241 aataagatgc tgtcactttt ggttctcgag gttgccctac tctacatcta gctgatagat
     6301 atgctgaaag aaacacagcg acattatgtt ttgtgcgttc catatcaaca tctttcgcgc
     6361 ttggtgtctc atatttcttt acatcgaaaa gtaccatcct ctgattcccc caattatgat
     6421 ataatactta tgtcggaaat attattcata gtcggaggaa tccgactttt tttattttgt
     6481 acgtgaaatg agttctcctg atttatacgc ctcagcaaat tcaaccaaag ctactgcctt
     6541 cattctctcg atagttctat cggaatatcc taatttttct cctatttgat acattgacat
     6601 cttattagga aaacaataac tataataaag tacctggaaa tggattctac ttagccgtgt
     6661 taatgcttcg ataaccgaat ctcgagtact tatcgcttca atgatttctt cgttttgact
     6721 gtcaattgtg aatttcaaat ttttggtaac agtcatcacg gaaaagtcta ttttcactgg
     6781 tccgaccaaa cgttctaaat ttctgtatct ttttagtaat cgtctagcat ttttccttgt
     6841 ttgcttaata tctatattct ctaaaatgtt cacaattgcc tcttctcctt ccagttgtgg
     6901 aataacgctc attgttgtag gatagttgta aaaaagcaaa attaagattt tataacttcc
     6961 atatccacca atctcaccac tgctaaattc tctttgcttt tcgctaaccg cttgtcacat
     7021 tccatcgtgt tttcaatgcg aatgatcgct gagtgattat agagatgctc tacatatcca
     7081 cgaaatggat agatgaaccc ttctgcttcg cagcgaacca tgtcaccgac tttaatttct
     7141 gattcctctt gctttatagg attcttagtt ggtagatcca tcatcaaacc accaattcca
     7201 taagcatcag tgtaaaatcc gtcttttagt ttcatctttc tgcctcccat ttacgatcat
     7261 catttaatat cgaaatccca aacttacgaa tagtctcact cgcatcagca acaaactgac
     7321 ttgccacttt atatgtttct tctgctgaaa ttccatattc tttttcaaac tttgtcttta
     7381 gtacattcag ttcctgtttt cttagttttg ctactctgcg gtgtctggtg ttcattgtca
     7441 atcaactccc taatctgaaa gtgtcgttta tacttgatcg aaattctttc aagtggttct
     7501 ctaccacaga atcagtcacg ttaaaacgat caattaatac tggagctgcc atatctttca
     7561 aataactttg tctgatgact aattcagtac catcaggaag ttttttatta acctcccgac
     7621 cattgataat tgcttgaatg cccgcttcac ttagtggtat ttcgtatttc attccgcttc
     7681 ctccttcaac cataaatttg gaaaatcatc ggcatatatc tctattgcgc caaataactt
     7741 gatcgctgat tttgattttc catgcatgtc aggactgatt aaacgatcat aataatcctg
     7801 actgattgtt tttcccttgt actgataacg tgtagccgca gtgtaatata ctgctgtctt
     7861 gctcagataa agatattttg ctaaatcagc cgatatccgt aacaaaatga ttctattctt
     7921 gcttttaatt ccacaattta gcaatgctgg gacattccca ttttcatctt ttaaagaatc
     7981 gatgtagtca gtagctaaga taatcactct acctccaata actcgctatt ctcgtatata
     8041 tttccgatga cttcatgagc agttgctaca tcataacttt cgccagtatt cctatggcct
     8101 aatctataat cacttttctt tagtacaact acaaagccgg cttcttgttg aagatacgca
     8161 acatatccaa tcgctccttt tgatgtaatt actacatccc cttcaaatat ctccacacca
     8221 ttcttatctt tcattcctgt ggattgcatg aggtcgatgt ttcgcacgtg atatgatttc
     8281 aattcggtag gatttatcca atattcaata aagtttgttt tgccgttttt agtaaaatgt
     8341 aagacagcta catctctcat tgtgttttct cgcttatccc acgctctaaa cttcggtatc
     8401 atttgctgtc ctcctcgtat ttttcaatca attccattac ttttttcact atttcaaact
     8461 caaccgcttt tgattcttca aaatcatgta caatttctgg atatagcata tcgtcaacta
     8521 cccatatgat aatttgacgt tcgtcagcaa aacttatgat taaatgatca gactccacag
     8581 atactgttgc tcctgattcg atatcatata aatccatgct gaattgaatg agctttttta
     8641 tcattcgctg tcctcctcaa atactcttct aatatttctt tatacttctc tacaaatttg
     8701 aaacgatctt gatgaagttt cttgctccaa tttgtttgcc gatccagctc acgcatctga
     8761 tcgaaccctt tttgaatttc gttgtaataa aattcaatgt ttgctgctgc tttccaatgc
     8821 ctgctacttc gcactcctac tcctgtttca gccatttcca acttaactaa ttccgctcgt
     8881 tcttttgctt ttttatcttt ctgaatcttt gccatgattt tcttgaggat gatatcactg
     8941 tattgtgtaa tgagatccat tatttctcct cctaaaattt catttcatcg ttgttgtcat
     9001 cttctttgtc atcagtttgg gaaaggagcg catacacgag atatgctgct cctactaaac
     9061 ctaagaacaa gagaatttta gccataaaga atcccatatt atttgcctgc ttcatccgta
     9121 actacagtat ctgctccatt tactgttacc catccatgct ttttgcgagc ttgagcttct
     9181 tcataacgaa tcaaattgtc tgttactgat tcggcgactt tgcgattaga ttctgcctct
     9241 gcttctgcag cttttgtttt cttgtaagct tcactatcag cttgagtttt tgcagtttct
     9301 gcatctagct tcgctttttc attttcttga ccagctcgaa tgatcgcatc gattgatttt
     9361 tgtgtttctt tatcgacatc tggaacacca agtgttacgt cttcgacttc aaacccttta
     9421 gattcaactg atttagcaaa gttcgttaat acctcagctt caactttaga ggaatctcct
     9481 gaaaggacat caagcagact atatttcgca taaacttcac gagcgacctt ttgaagctta
     9541 gattttaacc atccactttc gatatcttcc gaagtgatat tcccaaattc cttgtacatt
     9601 tttgctgctt tagttgaatc aactttgtag tcatatttga tatcaatcgt tgtctttttg
     9661 ccgtcgcttg tagatactga aatatttttt gattggatag tttgcaagcg aattggatat
     9721 tggatcactt tgtcaatccc tacaaatttc acaccttgcg tcagtgcttc atctttgata
     9781 ccgccgttca ttgaatagcg cacacccaca tatccattat cgattttttc gaagaactta
     9841 aatcctccga taactccaat acctacgatt actgctcctg ctacacctaa tttgattaat
     9901 ttgttttcgt tcattttttt cctcctaatt cttcatccaa aacataaatc gcaccaaatc
     9961 ttcttgtgta ccatttacat aaagattcag ttctgctcca actgcaacta gaattccgat
    10021 aattaacagg acaatacctg tcactgtata ttttcttcct tttctacttt cacagatgag
    10081 gtagtatcct agaaatgcta gtaaaccacc aatcgcacac caaaatattg ttaaacctat
    10141 catttgctgt cctccaaatc actcgacttc acgaatacac catctaccat ttttcctgtg
    10201 cgccctttga tttcgttgta tgccatttct aaacactctt gtacgtttgt ccctttttgc
    10261 atggaaagga taatcaacgt gacgattacg tctcctacgc tatctttaaa tagctcatca
    10321 ttacttcttg ccattgctga agcgatttcc ccgaattcct cagctacttt caaaaactgt
    10381 gcttttggat ctgcttgatc cagtcccttg tctttagccc actgctctac ttttgtgatt
    10441 agttcgttca ttttttctcc tccacatacc taaattgtcg tccttttgaa tcaatccata
    10501 agctccttgc tctatcccag atgatgtttt tgctcagacc agtaatttca
//
LOCUS       DAEGIQ010000092        10421 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000092 DAEGIQ010000000
VERSION     DAEGIQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.094"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            103..1146
                     /locus_tag="KMT45_002162"
     CDS             103..1146
                     /locus_tag="KMT45_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBE7005614.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1319..1543
                     /locus_tag="KMT45_002163"
     CDS             1319..1543
                     /locus_tag="KMT45_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005615.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1546..1803
                     /locus_tag="KMT45_002164"
     CDS             1546..1803
                     /locus_tag="KMT45_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005616.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            2020..2895
                     /locus_tag="KMT45_002165"
     CDS             2020..2895
                     /locus_tag="KMT45_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005617.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            2905..3699
                     /locus_tag="KMT45_002166"
     CDS             2905..3699
                     /locus_tag="KMT45_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005618.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3728..4846
                     /locus_tag="KMT45_002167"
     CDS             3728..4846
                     /locus_tag="KMT45_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005619.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4879..5199
                     /locus_tag="KMT45_002168"
     CDS             4879..5199
                     /locus_tag="KMT45_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005620.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            5454..6143
                     /locus_tag="KMT45_002169"
     CDS             5454..6143
                     /locus_tag="KMT45_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HBE7005621.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            6144..6347
                     /locus_tag="KMT45_002170"
     CDS             6144..6347
                     /locus_tag="KMT45_002170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005622.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            6367..7995
                     /locus_tag="KMT45_002171"
     CDS             6367..7995
                     /locus_tag="KMT45_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005623.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVIFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            8046..8417
                     /locus_tag="KMT45_002172"
     CDS             8046..8417
                     /locus_tag="KMT45_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005624.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            8502..8702
                     /locus_tag="KMT45_002173"
     CDS             8502..8702
                     /locus_tag="KMT45_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005625.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8836..10062
                     /locus_tag="KMT45_002174"
     CDS             8836..10062
                     /locus_tag="KMT45_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005626.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
BASE COUNT         4071 a         1263 c         2093 g         2994 t
ORIGIN      
        1 aacaacgatt atgatcttaa aacaatcgtt tatgtaattg gcaatcttac gaataatttt
       61 catgcaataa tgacaataaa aaatgaaaag gggtaaaatc aaatgaaaaa atttttagta
      121 ggaattctat tcacaatggg gctagtatta ttatcacctt cagtttcagc agaaagctct
      181 atttccatgt atcgtatgta taatcccaat agcggagagc atctatacac tagaagcgtt
      241 ggagaaagag acaatctgaa acgttatgga tggcattacg aaggtgtcgc atggcaagct
      301 ccaacaagtg gtactcctgt ttatcgtttg tataatcggt ataacggtga acatttttac
      361 acagttgatg ccggagaaag agataagatt tcacacacag gatggacgta cgaaggtgtt
      421 gcgttctatt cctacaacgg agcaaataga gttcctgtaa cacgtttata taacaaatac
      481 gtgaattggc atcactacac gttagatcaa aatgaaaaga acgtaattag taaacaaggt
      541 tggaagattg aaggtacagg ctggtatgca atgccaaaca gcgtaccaaa tcctacacct
      601 gctccaagca ctgaaaaagt gttgaacgtt cctgttgtat ggcaaggaaa tacaatgtta
      661 tgcgaaggag cttctttatt agaaggttta cactacaaag gtgtagccac aaatcaaagc
      721 ttaaccagtt ttgtaaacta tatgccaaaa gctaatgaca ataatcctta tcatggttac
      781 tcaggagagt ggcgtcataa cgttaatgga acgtatcaag gcatgatggc tgacccagta
      841 gttaaatggg caaaaaacgt tggcggtaat gccgtcaata tcactggttc aggaacgaat
      901 ggaatcaaga atgaaattcg caatggtaat ccggttgtaa cttggattac ttataactat
      961 gctacgccag agttcaaaca aatgccttgg ggtcgtgcag tatggaatgg acatgtagtt
     1021 ctcgtggatg gcttcagaaa tggtgcatac catgttgttg accctgtatt tggcgcaaaa
     1081 tggatcaatg caagcacgta tgagagagca tttaatgtga ctggaatggc ggttgctgta
     1141 cgttaattga tagaattatc aactaaatga aataagagct taattactga aatggtaaat
     1201 aggctcttat ttttttagag gaaatttttg taatacgaat ctataacaaa aaatattgaa
     1261 tgtagtacac aaaagtggta tattaaaatc gtagagatta tgcgaagagg tgattttgat
     1321 gaaccaaaga agaagaaaaa ggttagaaga aataagcgag cgactttccg agctaaagga
     1381 tgagttagaa gtaattcaag aagaagagta caaagaaaat atacctaaaa atctacaggg
     1441 atcagaaaga tatgaaaaag cagaagaatc cgttgatcaa cttgaagaag ctgttgatgg
     1501 tttatccgac atattagaat tgattgaaga tatagctagc tagagatgat taatgaagat
     1561 ttcaggagtg agaataaaat gattgatatt tcactgatag agaagctact tttttcaaat
     1621 aagtacacag ccaaagagtt ggctacaaga tggcaaatat ccgaatcagc tattaaagcc
     1681 tatcgtcaaa ataagggaag cgtaggatgg cgtgattgga aaggaatcag cattggaaaa
     1741 gcagaagaca taatgaaacg tgaaggattt caagctgaca atgattctaa aaaagttgaa
     1801 tagtttaata gtcgccaaga aaagtaccag gacgagggga aaggtagatt acaaaggaaa
     1861 acacttttaa ataaacttcg caaaagcatc aggttatgag tggctagggc tatacgaatg
     1921 tgatgaaaac aaagggcttt aatgtcaaag aaaacgcaat agggggggaa caaaattttt
     1981 aaaaaagata tttatgtttg gtttaatgga aaaaaatata tgactactaa caaaaggtgg
     2041 tcagagctag tacaaaatac agttaaaaaa gtcatgccag atggatatca tttcaactat
     2101 atacaatgct cacaaacaac tgaaagtatt tatttttcaa tattaaatga gaatagactc
     2161 tattatttca gggtaagttc acaccatagg aaaaagagag aagataatgt tacgacattt
     2221 ttatcgggaa aatacaatgg cttcaaagag ttaagtgaag ccattggtga gtatctaaaa
     2281 agtaatagat ttgtcagaat ttctgacaaa catatattga tcttgttact ttatttgaat
     2341 aatagcttca aattgttttg gagagacgac actttctact atattgagaa cggcatggat
     2401 atttattctg gtatcaacgg catattaaaa cattatatta aggcgttact tcgatatggg
     2461 gttctttatc aagaagattt aagtaaccaa attaagatta gtaattctgg aaagtcatta
     2521 ctggcaaaat ataaaaaatt gctttctcct atctacaaag gaatagatat ggaagctgta
     2581 aattctgaaa ctttatttaa tttagttgtt gatgaaaagt ttgcgaatga attttttaaa
     2641 agtaaaaaaa ttgaaagact aaataacctg tttcctgaaa atgcaataaa agcttatgta
     2701 gataataata aatcacgatt atacaactgg tttattccca aacggttgcg aggaaaaatc
     2761 aaaattggcg atcaagtgaa agtaattaat ctaaatcata ctaagatcgt taccgtaacg
     2821 gagttgtata tagctgaaaa agaagatatt acaaaaatga aaccagttat taaaatacta
     2881 gataacaaag agtgagtgga aaatatggta gcaaaaaaga atactctaaa agaagcttct
     2941 gaaaaatatc aagatatgaa aagacgagga ttaataccaa aggacaaaaa aggtatatgg
     3001 agagaatttg ttaaggaagt cagtagtgat cgaagtggcg gtgactttgt tggacgatat
     3061 acagcaaaag gttatagcag gcgcttaagg atcactgttg aatatggaac gaatgaatca
     3121 gctacagagc tgaagcaaag gtacttagat acacgaagag aagcttcaaa aaaatatgat
     3181 gatttcaaga gcagaggtct aattcctaag aagaaaaagg ggatttggcg tgagtttttc
     3241 aaagaagaag atagcaatcg gaataaggga gacttcattg gacgatacac agcaaaaggc
     3301 tacactaaga atttagggat caaggttgaa tacggaactg gtgaaagttc atgggaattg
     3361 agaaatcgct atttaaatac attgttagag aaatataaag cagaattatt ggagttgttc
     3421 gatgttaatg attttatcga gatggctgaa aagtatggtc atgaagaagg ttttatgatc
     3481 aatgacatta tggttattga taattttacg gatttattag cacttgcgaa aaaagcaagg
     3541 gatttaagag aaacgcagat gtatctattc agaaataaaa acggaagaaa ccggagaact
     3601 gaatcgagtg agattgaaga ttactatgat gcctttacac acgatctggg agcctttgta
     3661 gacgagataa gtggtgtaat gaacaaatat agggtctaga ataaaaaagc cggagatgag
     3721 cagataaatg ggaaacaata gcatatacac acctgaaatg cagaataaaa ttaaaagact
     3781 tgtcggatta gggtggatga caagaggaaa attagattct tttagtacga tcaaatatct
     3841 tttgatggac atttaccgtc agaacaagca aacaggaata cattttttgg gctatgataa
     3901 gccggaagat gcacctttct ttgttccaag ttcagcctat agagccagta cctccggtag
     3961 catgtataac ttggatttgt ttgaggaagg agcaaagatg atcgaatatg atttcaacga
     4021 agcctattcg aggatcatga gaaattatcc tttgccgtca aacacttatt tgaaagcaaa
     4081 atataataca gatcaagcta ttttgagagc gataaataga ccaaaagtaa gaccgtatga
     4141 aaatttaaaa acgtatatgt tctttttggt taattttgaa ggaacagcca aagaaggaac
     4201 atatgctaat ccaaaatcac agtcgatgtt tgcggattat ggcgaaaatc ccaaaacagc
     4261 taatgagcct atttggttat cggagattga gctaacactt atccttgatt attatgatat
     4321 taaagagatg gaagtcctag atagccacac atttttgacg aaaaaaggat tactcaaaga
     4381 atattttgaa agaatcgagc cattaaaaac tgatcctgat acgttgaagc tttataaaaa
     4441 tcttagaacg aaagtatttg gtgctatcgg acaaaaatca ttaagagaag gagaaacaag
     4501 caccttcgat aaatacagta tgtataatcg agcgtattcc tccatggtag caggcgtatt
     4561 tagagatcgt atggttagat atgaacagaa atacgttcat agcgcatatg ggctattaaa
     4621 aattcgtact gatggattgt atttcagatc cgaagtacca gagtttgaag agctgtataa
     4681 acttggagta gttaagaaaa aagttcatac aatatccaaa gaagaagtcg aaaaagcttt
     4741 agaatttaaa aacaaaggat ttgaaaataa gggctccttt gggaaatcta aagatgaagt
     4801 taagggtgtt gtgatagacg atgatttcga gtttgatttt gactaaatac tctttggaaa
     4861 agatggtagg aggtaaatat gaagaagaaa attgtggaag gaaatgtagg tgttattatt
     4921 ctaaaagatt caactgacta tgctaaatca atggcactaa aagacgaaaa agttgcacgc
     4981 atggataaag atttagttga atttctgatg gatgatacag aaagattaaa aggggatcag
     5041 tttttgagtt taatgaaaga caaaggctta aacacatatg gtttatttga ttataagaaa
     5101 ttagctatag agtgggtaaa agaaggagaa atatttttta ttgaagaatg ggaaggtact
     5161 gagaacttag ttcttcatca gtatcttttg aaagcttaat ctaagtaaaa cagttctgac
     5221 tagttatgct tgtcagaatt tcaaaggaag ttttccataa ccatcatcat tcaaaaataa
     5281 aggatttgaa aataagggtt cctttgggaa atctaaagat gaagttaagg gtgttgtgat
     5341 agacgatgat ttcaagtttg attttgacta gatttttttg caatgtttaa gagtaaacaa
     5401 ggtaagaact aagatcatga agattgagag taaagaagat agaggacgaa tgtatgagaa
     5461 cacactatat ttatttagaa gataaaattg tagatgaaat tccggcaaaa agtcgagaac
     5521 aagctagaaa attatttatt gaaaaataca aagagatcaa tggagatagt gaagttccaa
     5581 aagtggtcgt aacagttaga aaaaggcaaa caaaaaagag aagtacgttt catgtcagcc
     5641 ctgatatata taatcttata gagcgacata gaagtgaaga taatatttct atctctcaat
     5701 atattgctac agttattgat gaattgattt caggaaagta tgactatgct tttgattttg
     5761 agggtgaaaa ggattacaaa agtgttgctg tctttcaatc tgattttgag aagctaaaaa
     5821 actatgctgg caggagaaat gttaattttg caagactact agaagagttc tactccgaag
     5881 agttgggagt aggaaatgaa tacaagagag aaacttatgc gaaattgtct cataagttat
     5941 cccaagcgct taaagaagtg acagaagtta aagcggaact agatgctaaa cttgaagaag
     6001 tgaaaaaata tcaaacagag aggaagaaca acatgaagaa tcaagtggaa tttgacgaac
     6061 agaaagaatt tgaaaaagca agaaaacgct ataacaaaat tatgaatgaa cttagctatg
     6121 aacacttaac agttcatgaa taattgagtg agggtacaga aaaatatacg atcgcagata
     6181 tggttaatga agtggaaact cttttagctt ctttctttga aaatggtcac gtaaacgatg
     6241 aatggcgaaa agaagaacct aaaagggtta acgcttatat tgctcgatta aaaaggttta
     6301 ttgataaata taaggagaat agtgacaaat tttctaaaga taaataagga gagagtgtga
     6361 ttacatttgc cagaaatcga aggaatattt tcgccaaaaa taggtaaaaa acagttagat
     6421 ttcagcagtt ttccgagcgt aaatagagat tataatctag gtgatgaggt acagatacta
     6481 aatattgatt tcaaagaact agacttcgct gttgctgttt ccaacaaacc caaggcattg
     6541 gaacgagtga aaaaaatata ccaagagaat gatcaaaata tcaaaaatat gatcaaaaag
     6601 tatagacgag ataattcaaa agttgaagac gttttattca acggtacggc aaaacatgat
     6661 ttgaatgttt atatggcatt aggagtggct ttgtactgta attcaattaa tcagacagat
     6721 attttggtta atatcgtgag gaatagctat caaaaaatat taaaaataag tagcgaaaca
     6781 aacccttatt atgcaatgaa atatcttggg gataatttat taaatgataa aaaattgatg
     6841 cacaacactg aagagctgtt atctaatttg gctgcgatta actttctatt caaaacaggt
     6901 tctgaagatt attttataga taacgtacta aaaggtgctt acggaacagc attttatccg
     6961 ctttacaata tgaaacagca taaagccgaa caaaaggata acaaacttga aattaaaaag
     7021 atgaaagaac tattaggtat aggatttgta aaacccaatt caaaagtcac tattgcttcg
     7081 ttagcaacta ctaacaatga tgggaaaata gtaaatgggt ttgacgattc agatatagat
     7141 atattgtcaa aatcatattt ttctttgtat ggagattttg cagatttgat gggtattgat
     7201 agttacttag tcaataattt tgagatcaag ggtagcgagc tgaatgatat tgtgtatttc
     7261 gttttaatct ctctatatgc aattgatcag aataccgagt accgacaaat tgactatttg
     7321 ctagtttttt atagtttgtt atatcagttc gttttaactc ggttgtttaa aaatgctaag
     7381 gaagaattga tcctgaattc tgattatgaa atagcaaaac aaattgcaag tattagaaaa
     7441 gaaaacaaat ctgaagtaat caaattaaga aaagaattaa gtattgcgag taaagaaaac
     7501 ggtcggttag aatctttagt caaatcggca aaaaaagaag aggaactttt gaagcaagag
     7561 attaaagaat taaaaggaat cagaaaagaa cttgaacagc tgaggtttga aaataaagta
     7621 ttaaagcaat tgcaggagca agaacataga caatcattaa atggtaaaac tgaagagcct
     7681 tcactgtctg aaatgatagc ttatctaaaa cataaaaaca ttcttttagt gggaggacat
     7741 gtaatttttc aaaataaatt aactgaacta ttaccaaata ttaaaatgtt tgatgtaagc
     7801 gaaaataatg gaaagatact taaaaatttg aatacgtgtg atgcgatttt tctatataca
     7861 gattacttga atcatggtct atattatcgt gttatgagtg aaatttctaa taaagataag
     7921 atattttatt tggataatca tgtgaatatg aacttaacaa ttaaacgtat ttacgatctt
     7981 ttatcactta tataaagaga gaagtgataa ataggacaca aaatttggaa agtaggtaag
     8041 tcactatgag agaactaact ttatcttcta aggaattaga aaagaaaact gctttattaa
     8101 acgaaatatt tagtgctgat tgtataatcc aaagtttaaa agaaagttca ttttcaaaac
     8161 taacagatca ggaaaaaatt tcttatatgc taagtttaga taatctagga actaaatatg
     8221 atgaatcagc aagtagcgat aagcttttga ttgaattagt atcaagtctt agtagttttg
     8281 gaaaaatgaa aagatttaat gtgatattag aaatttttgc ttggttaatc ccttcattta
     8341 tttggttttt ttatattttc atagttgaat ctgagttaat agccaaggtc tctcttggac
     8401 tgttaattcc gatataaata ggttcagcaa tttactttaa taacttgtat acacgtattc
     8461 aaataaaaaa gcagttatta catgaaggat taagtggtaa tatgttaaaa agaattgcat
     8521 gggataaaga taataaaaca gatattgagc ttaaagaata taaatacaat tatttctcga
     8581 gatgtataga tagaaagtta ctgtttaaaa aagaaggatt agaatttagt tttgatgaag
     8641 atgaatttga aaaattagtg atggactggc gtaagaattt actggatacg gtacaattct
     8701 aaaagcaatt gaggtcgaaa ttccgatact tgaatcatga tctatattat cgtgttatga
     8761 gtgaaatttc taataaagat aagatatttc atttggacag tcatgtgaat atgagcttaa
     8821 caattaaacg tatttatgat cttttatatg tttataaaca ttaatgaggt gctaaaattg
     8881 acaacaataa ctatgacaaa aaaaatagaa gtatatgttg atggatcaca agaagaaagg
     8941 gattcctttt ttgatttttt ttggaaaaat agtagagaat atgtaaaagc gttgaatctg
     9001 gtatataaac agctaatgtt tgaagagttg ataaaagatg caattaaaga aatggatcaa
     9061 ggttatgctg agagagaaga aagtattcaa aatcggattg aaaagttagc atatactgat
     9121 ccaatagatc aaaaaaaact tgataaagca aaagctgaat tatctaaatt gagaaatgaa
     9181 aaaaataaag aagcgagaga aatattaacg caagcgatag ggttgaagag tcaaacgaga
     9241 acacgagatg tagcaaaaaa attagacttt gactttggag attttgttga ttccgcaaat
     9301 atgaaagcta gtaaagattt tggcaatgat tttatcggaa ttgtgaccgg acaaagaacg
     9361 cctagaatat ataaaaatag aaacaaatat ggattactac cttttagagg tcgagatatt
     9421 aaaatccata aagatgataa gttttatatt tatttaccta aaggctttag gtttaaagtg
     9481 aatttaggta gcaagccaag aaaagcaaga gatgttgctt cagttttaca acacattatg
     9541 gatggcgatt acaaattaaa tcaaagtaat cttatttttg atggaagaaa attatttttt
     9601 aatctaacaa tttcttttga aaaaagctat aatgaaatgg aattcaagaa aggtaatgaa
     9661 ttgatcgtaa actttgcaga tttgtgtagc ggtgaatgtt taattaatga taaatctgta
     9721 atgtcttttg gtgatagcca atatataaga aacttcaaaa gtaaaatttt agcaatgaaa
     9781 tcaaaagaac aaagccgagg agtcttttct aagggcggtc atggtcgaaa aagaaaatta
     9841 gatcaagatc gatgggaagc aataaagaaa agagaaagta attttgttaa aacttataat
     9901 aatcaatgtt ctgcaaaaat cgttaaaact gctattcgca ataaagctca aattattcgt
     9961 atcattgaac cagataaggc ttatggcgaa tgggcttact atcaattgaa agaacaacta
    10021 gtaaacaaag gaaaaagata tgggatagaa gtcagaagtt agtaaattaa acaatcgttg
    10081 atttggcaac gaaatagtga gggaaaatcc actcactgaa gggtaaactc cgtcaagttg
    10141 tgtgaccaga cggaaggacg aatgttgttg aggttttaga accatataaa acagcatgtt
    10201 tctcaaattt ttaggataag ggaaattgct agcagtttgt tttagaacca tataaaacag
    10261 catgtttctc aaatgagtac tttaaattaa ttgatggcgt tcctgcttta gaaccatata
    10321 aaacaacatg gatttaaaac aagaagtaag tcagttctcc ttttacttta gtaaggaaaa
    10381 ataaagtatt ctcaataaaa cttaaaataa gcaaaccttt g
//
LOCUS       DAEGIQ010000093        10124 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000093 DAEGIQ010000000
VERSION     DAEGIQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.095"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(46..366)
                     /locus_tag="KMT45_002175"
     CDS             complement(46..366)
                     /locus_tag="KMT45_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBE7005627.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(514..2748)
                     /locus_tag="KMT45_002176"
     CDS             complement(514..2748)
                     /locus_tag="KMT45_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBE7005628.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3227..3493
                     /locus_tag="KMT45_002177"
     CDS             3227..3493
                     /locus_tag="KMT45_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBE7005629.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3493..5220
                     /gene="ptsP"
                     /locus_tag="KMT45_002178"
     CDS             3493..5220
                     /gene="ptsP"
                     /locus_tag="KMT45_002178"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE7005630.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRGLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5384..6430
                     /locus_tag="KMT45_002179"
     CDS             5384..6430
                     /locus_tag="KMT45_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBE7005631.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6447..7670
                     /locus_tag="KMT45_002180"
     CDS             6447..7670
                     /locus_tag="KMT45_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBE7005632.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7759..8646
                     /locus_tag="KMT45_002181"
     CDS             7759..8646
                     /locus_tag="KMT45_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBE7005633.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8732..8962
                     /locus_tag="KMT45_002182"
     CDS             8732..8962
                     /locus_tag="KMT45_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBE7005634.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9019..9984)
                     /locus_tag="KMT45_002183"
     CDS             complement(9019..9984)
                     /locus_tag="KMT45_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBE7005635.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3225 a         1786 c         2044 g         3069 t
ORIGIN      
        1 tttacttttg tcccacgctc tttaattatt atgttcaaaa tttgtttaca cggctaatgt
       61 agcgcctaat tcatgttttg gaatcgagat ttcaacaagg cctgccaatt cgattacttt
      121 gatttcatcc tcgtggtaaa cggcgtcttt gattttatct aagaaataat ttttagcatc
      181 ttcgatttct ttcggctgga ttttacgttt gtcattgatc agcagaattt cattatgttg
      241 aggggcttca gtgtagacaa tagtggtatt tccggctgtg tagaccttga ctagatgggc
      301 atcagtgttt tcaagctggt ttaatacaag acgggaatgg ctgtttgtta catttaccaa
      361 tttcatggcg ctcacctctt ttatgtattt ttaattgctt gtttctctta ttataatttc
      421 ttttttataa aatgaacagt attttgactt ttctgaaaaa agaatgagac ggcatttctc
      481 aaagaaatac cgtctcacgc tataaaactg gatttattca gccgtttctt cggctgattc
      541 tttagccaaa cgttctggtt tagaagtaac gatgattttt ccatctttat ccagttttgc
      601 tttcaactca tggatagaag ggtgatcaag ataaaattca gcgataccat cttcgatttg
      661 ttcttggatc gtgcgtcgaa gaggacgagc acccatagat ggatcatagc ctaagtcaac
      721 taatttttct ttgacattcg taggaacttc gatgtgaagt tgctgtgctg ccagcatttg
      781 gttgacatcg tctagcatta gtgtgacgat tgtcatcaag ttttcttttg ataatgcact
      841 gaattcgatg atcccgtcaa aacggttcag aaattctggt gtaaagtagt tgtttaattg
      901 gttcaataca gaacgagtaa caccttcgcg tgccgcacca aaaccaacgt ttgcttctac
      961 tttaccagtt ccggcattac ttgtcataat gatgatcgta tctttaaagc tgaccgttct
     1021 tccttgtgca tctgtcaatc gaccatcatc taagatttgc aagaacatat gcaaaacatc
     1081 tggatgagct ttttctactt catctaacaa gacaagactg taaggatttc tccgtacttt
     1141 ttctgttaat tgaccagctt cttcgtatcc gacataacct ggaggagaac caatcaattt
     1201 agagacacta tgtttttcca tatattcaga catatcgaaa cgaatcattg aatcttgtga
     1261 accgaatagt tcgtaggcaa gttgtttcgc aagctctgtt ttacctacac cagttggtcc
     1321 aacaaataag aaggagccga ttggacgatt ctgtttgttt aatccaacac ggtttcggcg
     1381 aatcgctttc gctactcgat ctacagcgtt atcttgtccg atgacatgag ctttcaagtc
     1441 atctgctaag tttttcagtt gtgtctgttc tttttctttt aattcaccca ctggaatacc
     1501 tgtccgttgt tcaacgattt tttccatatc tttttcggta atcacaggtg tttcctcttc
     1561 agtgagctga cgttctttca ttttttgaag tttattgatc tgatcgcgat aataagctgc
     1621 tttttcgaaa tcttcttctc gagaagctaa aactttttgt tcttctgctt cttgcagttt
     1681 tttctcgatt gttttaggat caacgatttg gatcgtcaag tttttcttag aacctgtttc
     1741 atctagcaaa tcgatggctt tatccggcaa gaagcgatct tggacgtaac gattggataa
     1801 agttgcggct gcttcgatgg cctcatcagt atattttaca tgatgatagt cttcataacg
     1861 tttttgcaaa cctttcaaaa tactgatcgt ttcttcgaca gttggttcat ctacccgtac
     1921 aggttgcata cgacgttcta aagcagcgtc tttttcgatg atgcggtact catttaaggt
     1981 agtagcacca accatttgca attctcctcg agctaaagct ggttttaaaa tattaccagc
     2041 atccatattt ccatcaccag cagaaccagc accgactatt tcgtgaactt catctatgaa
     2101 taggatcaca ttttctgctt gacggatctc ttcaatcaat ttttgcatac gttcttcaaa
     2161 ttgtcctcgt ataccagtac cttggactaa agatacgaca tctaaacgaa tgacttcttt
     2221 gtccattaat ttttgcggta catcaccatc tacgattttt tgtgctaaac cttcaacaac
     2281 tgctgtttta ccaacacctg gttctccaat caagacagga ttgttcttgg ttcggcgatt
     2341 taaaatttca atgactcgtt tgatttcgtc atcgcgtccg atcacaggat cgatatcacc
     2401 ttgtcgtgct tgttctgtga tattgattcc gtattctccc aataaacctt ctgtattgcc
     2461 ttgaggtggc tgaccaccat tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg
     2521 ctcataattt ccttgctgtt gcatttggcg agacatggaa cggaaaagat cgtccaaact
     2581 gccaaatcca aatggatcat tttgggccat attagtcaga cctccatttt gttgattttt
     2641 gattttttga taacagctct gacagtaatc gagttgtgtc cgctgaccat tgacagtggc
     2701 atacaagtga atcgttgcct cattcttgcc gcagttttga caaagcatga atcattcacg
     2761 tcctttcaca aaaatgggta catccatatt ttacatctcg tttttcatga ggtaaaagaa
     2821 taacccctag ttttttaaga gatttatgag gtagaaaagg tgaaatctct attgaccatt
     2881 cctgaccttt gactcttatt atactaaaaa taaaaaggga agcaagtaat tcgtttcata
     2941 tgaaaacaaa gagggtcaaa ggaaagaatg ataaacgtca ggagaagaga aagcgacaag
     3001 aaaaaggaaa taaacacgtt tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga
     3061 ataatttgaa tacttttatc tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat
     3121 gttaatcttt acagatgaaa gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga
     3181 aaaccctcac ataaaaacaa cactcactta aaggagaccg attaatatgg aaaagaaaga
     3241 atttcacgta gtagcagaaa ctgggatcca cgctcgtcca gctacactat tagtacaaac
     3301 agcaagcaaa tttaactctg atgtaaactt agagtataaa ggtaaatcag taaacttgaa
     3361 atcaatcatg ggcgttatgt ctttaggtgt aggtcaaggt tctgatgtta ctattactgc
     3421 agaaggtgct gacgaagctg atgcaatggc agcaatcgtt gaaacaatga agaaagaagg
     3481 cttatctgaa taatggttga aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt
     3541 gcaaaagctt acctgctagt tcaaccggat ttaacattca gtaaggcaac agtagaagat
     3601 actgcggctg aagaagctcg tttggatgct gcattagcta aatctactga agaattgcag
     3661 caaattcgtg aaaaagcagc gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc
     3721 catttgatgg ttctttcaga tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac
     3781 aacagcgtaa atgctgaatc agcgctaaaa gaagtaacgg atatgtatat cggtatgttc
     3841 gaagcaatgg aagataatgc ttatatgcaa gagcgtgctg cagatattcg tgacgttgct
     3901 aaacgtattt tagcgcactt attaggcgtt actttaccaa atccatcaat gatcaacgaa
     3961 gaagtggtcg ttgttgcaca tgacttgact ccaagtgata cagcacaatt agatcgcaat
     4021 tttgtaaaag cgtttgtaac agacattggt ggacgtactt cacattctgc tatcatggca
     4081 cgttctttag aaattccagc aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa
     4141 ggcgatattt tggctgtcaa cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa
     4201 caaaaagctg aatttgaaaa agcaggtgca gattacgcag cacaaaaagc agaatgggaa
     4261 aaattaaaaa atgcagaaac tgtcacagct gacggcaagc attttgaatt agctgcaaac
     4321 atcggaacac caaaagatct agttggtgtc cataataatg gtggagaagc agttggtctg
     4381 taccgtactg aattcctgta tatggattct ccagacttcc caacagaaga tgaccaatat
     4441 gaagcttaca aagctgtatt agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg
     4501 gacatcggtg gagataaaga actaccatat ctgcaattac ctcatgaaat gaatccattc
     4561 ttaggatatc gtgcattgcg tatcagttta tctgaacaag gcgacgaaat gttccgtaca
     4621 caaatgcgtg cattgcttcg tgcatctgtt catggtaatt tacgtatcat gttccctatg
     4681 gtagctacac tcaaagaatt ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa
     4741 ttgataagtg aaggcaagga agtatctgat acgatccaag tcggcatcat gatcgagatt
     4801 ccagcagcag cagtcttagc agacaagttt gcaaaagaag tagatttctt ctctgtaggt
     4861 acgaacgatt tgattcaata cacaatggca gcagaccgta tgaacgaacg cgtttcttac
     4921 ttgtaccaac catataaccc atcaatttta cgtttgatca aaaatgtaat tgatgcagca
     4981 cacgctgaag gaaaatgggc aggtatgtgt ggagaaatgg ctggagatca aacagctgtt
     5041 ccattgctag taggtatggg tctagatgag ttctcaatga gtgcgacatc tattcttaaa
     5101 acgcgtggtt taatgaaacg cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca
     5161 ttgaaagaat gcgatacgat ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa
     5221 acaacgtatc aaaagctggt gtggcttgta aaagagctac gccagttttt ttgttctttt
     5281 ttaagaaaac gtttttgtca gtaagaatag acaaaaatag ggcttaagtt tgagtgacag
     5341 tttgtaggct atgttatact atataagtga agggggacgt aaggtgaagg tattattata
     5401 ttttgaaagt gaaaagatgc ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa
     5461 acgtgctctg acggaagtcg gtatctctta tacactagat gaaaaagaag attatgacat
     5521 cctgcacatc aatacatacg gaatcaatag tcataatatg gtcaataaag ccagaagaga
     5581 aggaaaaaaa gttgtctatc atgcgcattc tacagaggaa gatttccgaa attcctttat
     5641 cggatcgaac cagctttctc ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc
     5701 ggactatctg atcacgccaa caccttattc aaaacagttg ttggaaagct atggtatccg
     5761 tgtgccgatt caagctattt ccaatgggat cgacttagag aaatatcacc ctgatccaat
     5821 gaaagaacaa aagttcagag aatatttcaa actgtctccg gatcaaaaag taatcatttg
     5881 tgtcggtttg tttttcgaac gtaaaggaat cattgatttc gtagaaattg ctaagaagat
     5941 gcccgaatac acatttattt ggtttggtca tgttcccatg tactcgattc ctcggaacat
     6001 ccggaaaatt gtaaaagaag atcatcctga aaatgtcctg tttcctggtt acattcgtgg
     6061 agagatcata gaaggtgcgt attcaggtgc agatctattc tttttccctt cttatgaaga
     6121 gacagaaggg atcgttgttt tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga
     6181 tattccagtg tataatggct ggttagaaga tagcaagaac tgctatatgg ggaagacaaa
     6241 cgaagaattt atccgactga tccaacagat taccaatcaa gaactaccga gtacgacaca
     6301 agccggttat caaacagcaa aagagcgaag tatcaataaa atcggtgaag aacttaaaaa
     6361 tgtatacgaa agtgtactga atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt
     6421 caaggactag tttggaggcg aaaaacatga aaatcggctt ttttaccgat acctactttc
     6481 cgcaagtcag cggagttgct acatcgatca aaacattaaa acaagaatta gaaagacatg
     6541 ggcatgaagt ctatattttt acaacgaccg atcctaatgc ggataagttg gaaaaagatg
     6601 tgattcgaat gccaagtgta ccatttattt ctttcaagga tcgaagagtt gttgttagag
     6661 gcatgtggta tgcttatttg gttgccaaag aactagaact agatcttatt catacccata
     6721 cagaattcgg agcagggata cttgggaaga tggtagctaa gaagttgaaa atacctctga
     6781 tccatactta ccatacgatg tatgaagact accttcatta tatcggtaaa ggaaaagtga
     6841 tcaggcaatc ccatgtaaaa tacttgtcta gattatttgc aaatcatacg acaggtgtag
     6901 tttgtccaag tgaacgtgtt attgatacat tacgcagcta tggtgtaata gcaccgcttc
     6961 gcgtgattcc tactggtatc gacattgaaa aattcaaacg ttctgatatc actcagcagg
     7021 atatcagcaa attaagggag tcactaggtc ttcaggaaaa ccatttgatg attctttctt
     7081 taagcaggat ttcttatgag aaaaatatcc aagccttgat taatgggttt cctcaggtca
     7141 tctccgctta tcctaacgcc agattagtta tcgtaggaaa aggaccttat gtagaagagt
     7201 tagaggaatt gatctcagaa ctgcagttag aagaatttgt gcagttcaca ggagaagtgg
     7261 acaacaatga tgtagcactt tattataaag cggcagatta ttttgtcagt gcttctactt
     7321 cagaaacaca agggcttact tatacagaag cgatggcagc cggaacacct tgtgtcgtgg
     7381 aaggaaatgc ttatttgaac cgattgttcg atcatccttc attagggaaa acttttgaaa
     7441 ccgacgaaga ctttgcacca gcacttattg attatatttc cagtgaagtt cctaaagatc
     7501 caaaagtttt agaagctaaa ctttatgata tttcatctac acatttcggc gaagctatga
     7561 tcgaatttta tcaagacatg gttgtttatc atgaagaaga gatgcgaaga aaagaagaag
     7621 aagactcgat tgaacgaatc aaaataaaat taaattcatt taaaagataa aattcaaaca
     7681 ttttttatga aaaaatcctt gtttctagcg tatactagca tagaagcaag gatttttgat
     7741 ttttaggagg tcgaaaaaat gaaacttggt tttatcggaa caggcgtcat gggaagtgct
     7801 gtcgcacgcc atctactaga agcaggacac gaagtagctg tctataatcg gacaaaagca
     7861 aaagctgatc ctttagtaac agaaggagct atctgggcag atacaccaaa agcggtagca
     7921 gaacaaagta atattctctt tacaatggta ggttacccca aagatgtaga agaaatctac
     7981 tatggacagt cggggatttt ttctgcagat atctcaggac atattttagt tgatttgact
     8041 acaagtacac caagcctggc agaaaaaatt gcaaaaacag caaaagaaaa aggcgcagat
     8101 gcgttagatg caccggtatc cggtggtgac ttaggagcaa agaatggtac actgacgatc
     8161 atggtcggtg gagaagaagc cgtttatgat caagtactgc ctttattcaa agaatttggt
     8221 acgacattta cattgcatgg ttctgctggg aaaggtcagc atacaaaaat ggctaatcag
     8281 atcatgattg caggtacgat gacaggaatg acggaaatgc ttgtctatgc acaaaaaaac
     8341 ggacttgatt taaagaaagt gattgagacg ctttcaggtg gaagtgcagc taactggtct
     8401 ttaagcaatt attctcctcg tattttaaaa gaagattaca cacctggttt ttttgtcaaa
     8461 cattttatca aagatttaaa gattgcatta gaagaagcag aaaaaatgga tcttgtattg
     8521 ccagcaacta cccaagcatt gaaactatat gaagagttgg cagataaagg ttttgagaat
     8581 gacggaaccc aagctttgat caaactttgg tggcctgaag gaaaaatacc agaaaaaaaa
     8641 tcataaaaaa cgagtttttt tagagaaaat caatgcaagt aggctatgct tttgatacaa
     8701 tataacagaa ataagaaaag gaggacctcc gatgacacat tcacaattag tcgcaatcat
     8761 aaaacgactg gaggctatgg tcgagtcagc agataatgaa ctgcaagttc gccgttttga
     8821 aaaagaaggc gtagagaagt gtacagtaac ttatgataaa gcgaccgata catttgaatt
     8881 aactgaaaca gatactcatc agcaatacga atttgataat atcgatatcg tagcgatgga
     8941 aatctatgac ttaatccaat aaattttagg ataattgaca tttgaagttg aagatgccta
     9001 aaagaagccg agaagcaact aatttagttg cttttcagct tcttttttgt ttaacagttc
     9061 aatttcaaac tgttcttttt ctaatttttt gcggtaactg atcaagattt gtacaggatc
     9121 gatttcttct tttgacaatt gacgtttcaa agataaatga gcttctttct tttcataata
     9181 gaagtcagga gaaacgtcac cagaggtaat cattgtttct gcaagttgac cataacaaac
     9241 gaggtagtgc tttttctgac tctcatctgc tgctaatggc atgctgaagg tgagcgatgg
     9301 tgttcttaaa aactgctttt gttcctggat ccgtctaata aaataagtaa ctaattcgat
     9361 atctttttga agcataccgg ctttttcgaa attcaactga tagatgaaat gttgttgtct
     9421 ttttttcaag taagtcagta gctttttatt tcggccttga aacagccctt ggagttcctt
     9481 ttttttctgt tcaaaagtca tttcctgtag gtcaggaagc tggactcgca aagctaacaa
     9541 actaatttca gacaaccaag gcatttgata agtttcttcc attatacgca aaatagacgg
     9601 cattttttta tagagacgaa aaggcccgta tgttcgagcc gtaggagtat ctgttaatac
     9661 aaatccctta ttggtaatat gtacataatt ataattactg aaataattca tttgtcggtt
     9721 ataaaaagga cggtaatgtt ggatcaattg acactcgagc aataaagcat ctaattccgt
     9781 gtctactacg acgaaatcaa aatctgttat atgatgaatc atacgtaata ctttttttga
     9841 atgctgttta ttctgatgaa aatagctgct tactctattt ttgagccgtt ttgctttacc
     9901 aacatagatg accttaccat ctcggtcttt cataagatag acacccggca gcatggggag
     9961 agttttcatt ttttctttta acataggatc tccttttacg ctttgaataa aaagactata
    10021 acaaattttg aaataatgga caagttttag ggaattctat agttctgttc acagaaaata
    10081 ctttgataat caaaatatgt gagatagatg atttttctaa taaa
//
LOCUS       DAEGIQ010000094        10088 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000094 DAEGIQ010000000
VERSION     DAEGIQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.096"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            185..1183
                     /locus_tag="KMT45_002184"
     CDS             185..1183
                     /locus_tag="KMT45_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBE7005636.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="KMT45_002185"
     CDS             1295..1804
                     /locus_tag="KMT45_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBE7005637.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="KMT45_002186"
     CDS             complement(1834..2493)
                     /locus_tag="KMT45_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBE7005638.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="KMT45_002187"
     CDS             complement(2495..3259)
                     /locus_tag="KMT45_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBE7005639.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="KMT45_002188"
     CDS             complement(3267..3944)
                     /locus_tag="KMT45_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBE7005640.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="KMT45_002189"
     CDS             complement(3981..5366)
                     /locus_tag="KMT45_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBE7005641.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="KMT45_002190"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KMT45_002190"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="KMT45_002191"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="KMT45_002191"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBE7005642.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="KMT45_002192"
     CDS             6305..7162
                     /locus_tag="KMT45_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005643.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="KMT45_002193"
     CDS             complement(7243..7578)
                     /locus_tag="KMT45_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005644.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="KMT45_002194"
     CDS             complement(7645..9015)
                     /locus_tag="KMT45_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBE7005645.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="KMT45_002195"
     CDS             complement(9093..9629)
                     /locus_tag="KMT45_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE7005646.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..>10088
                     /locus_tag="KMT45_002196"
     CDS             9938..>10088
                     /locus_tag="KMT45_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005647.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLK"
BASE COUNT         3029 a         2060 c         1666 g         3333 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagc
//
LOCUS       DAEGIQ010000095         9949 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000095 DAEGIQ010000000
VERSION     DAEGIQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.097"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(336..1184)
                     /locus_tag="KMT45_002197"
     CDS             complement(336..1184)
                     /locus_tag="KMT45_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBE7005648.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1183..2250
                     /gene="gnd"
                     /locus_tag="KMT45_002198"
     CDS             1183..2250
                     /gene="gnd"
                     /locus_tag="KMT45_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBE7005649.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKRTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2252..3253
                     /locus_tag="KMT45_002199"
     CDS             2252..3253
                     /locus_tag="KMT45_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBE7005650.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3250..3534
                     /locus_tag="KMT45_002200"
     CDS             3250..3534
                     /locus_tag="KMT45_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBE7005651.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3567..4364
                     /locus_tag="KMT45_002201"
     CDS             3567..4364
                     /locus_tag="KMT45_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBE7005652.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4378..5211
                     /locus_tag="KMT45_002202"
     CDS             4378..5211
                     /locus_tag="KMT45_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7005653.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5229..6086
                     /locus_tag="KMT45_002203"
     CDS             5229..6086
                     /locus_tag="KMT45_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE7005654.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6108..6617
                     /locus_tag="KMT45_002204"
     CDS             6108..6617
                     /locus_tag="KMT45_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7005655.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6654..7055
                     /locus_tag="KMT45_002205"
     CDS             6654..7055
                     /locus_tag="KMT45_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005656.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7167..7520
                     /locus_tag="KMT45_002206"
     CDS             7167..7520
                     /locus_tag="KMT45_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005657.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7554..7997
                     /locus_tag="KMT45_002207"
     CDS             7554..7997
                     /locus_tag="KMT45_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBE7005658.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8014..9099
                     /gene="uxuA"
                     /locus_tag="KMT45_002208"
     CDS             8014..9099
                     /gene="uxuA"
                     /locus_tag="KMT45_002208"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBE7005659.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9092..9805
                     /locus_tag="KMT45_002209"
     CDS             9092..9805
                     /locus_tag="KMT45_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBE7005660.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3328 a         1571 c         2181 g         2869 t
ORIGIN      
        1 acaaatgata acctaaaatg aggccaaaag ccagtgttat caaggaaata cttaattcac
       61 caaaattttt tttaattttt tagaaaataa gaaacaaaaa taataaaaaa aagaaaagat
      121 aaatttttta ctactgtata ctaactttgt tatcgcatct aacaagtaac tcgaataata
      181 ccaatacaac cttctccaaa gatgcgatga catacgttct aacccaccga ctattgatgt
      241 tctgtaccac ccttgcagaa ccaaaaaagc cactaccaaa tatttggtag tggctttttt
      301 tgttttatgg atttttcgct ttttctgtta tggtgctatt ctttgatttc agaaatagta
      361 tgtcgaactt tagcgataga acgacgtgat tctttttcgt tgtaaaacat caaaatcaca
      421 tatagagagt ccaaaatgct tagttgagaa atacgggaag ctaaagcttc agatcggaat
      481 tctgtttctt cagaaataga aataaaaacg acatcgccaa gtttagcaag ggaagaatta
      541 gcttggctag taataacgat tgtttttgct ccagtttttc ttacggtttc agcaatatgg
      601 attgtttctt ttgattgccc tgtgtgagaa atcaaaacag cacagtcttt ttccgtcaat
      661 aaagaagctt ccatcaattg aatatggtaa tccgtgctgt gagaagttgc gataggggaa
      721 cgcaaaaatt tatgataagc gtcagttgct acaatctctg aaccgccaac accgaaaaaa
      781 tagacacgtt tagattcatt gataatagta gccgcttttt ttagatcctt ttgttgtaac
      841 aacttttttg tgtcatttaa agttgaaata ctagactcga acaccttttg cgccatagtg
      901 agctcagtat ctgttttctg aatattttcg tggatagaaa tagcagatag atcattttct
      961 tggatcaaca tggccatttt aaaatcttta aatccattat aaccaagttt ttttgtaaat
     1021 tgaaaaaaag tagagtcagc aatgcccagt tcacttgcta aattgctaat aggactatga
     1081 cttactttgc ttggattttc taaaatataa tcggcgatag cctgttcttt tgggctgaag
     1141 tcgttgtaca ttgttttgat ttgtagtctt atggagacgc tcatgagaat tcctcatttt
     1201 cataataaga ttatcatttt ttccgttctc attaatgtta aagatacaag gagagtagga
     1261 aaaaatcaaa caaaaaatat ttttgtaaaa tctatttaca aaaaaatttt tttgtataat
     1321 gagggtataa gaaatggagg cgctatcatg gaaattggtg taattggttt gggaaaaatg
     1381 ggattgaatt tagctttaaa cttaaaagag caccatcaca aagtacaagg actcgacatt
     1441 tcaagtacag ctgttagtca ggcaagagaa aatgacattc ctgcttatca agagatctct
     1501 gaatttctag ctacttttga gcagaaaaaa atcatctggt taatgcttcc tgctggagag
     1561 ataaccgaaa acatgttaag aaaacttttt ccaaaactcg gaccaggaga cattattatt
     1621 gaaggcggaa actcttatta taaagattca atccgtcgag cagaagagtt tcagaaaaat
     1681 gaaatcggtt acttggattg tgggacttct ggaggaatcg aaggagcacg acatcaagcc
     1741 tgcttaatga ttggaggaga taaagaaact tttctatccg tggaacaatt attcaaagat
     1801 gtagcaattg aaagaggata tctgtatgct ggtgcatccg gaagcggaca cttcttgaaa
     1861 atgatccata atggaatcga atatggaatg atgcaagcaa tcggtgaagg attccaactt
     1921 gtacaaaaaa gtgagtatgc ttttgacttg gaacaagtcg cacgtgtttg gaatcatggt
     1981 tcggtgattc gcggctggct gatggaaatc atggaagatc agttaaacga acatcccaat
     2041 ttaagtaatt atcggggaat cgtttctgca tcaggtgaag caaaacggac ggttgaaaca
     2101 gcgctagaaa tggctgttcc agttccagta attgcaacta gtttgttcat gaggaatctt
     2161 tcacaggaag aagatagttt ttcagcaaaa gtagtttcag cactaagaaa tgggtttggc
     2221 ggacatgaaa tagttgaaaa gggtgagtaa aatgccagat tatcatatag caatcgttaa
     2281 ttccagcagt ttcggaagag tctttccaca ccacttggaa agattaaagt cagttggaga
     2341 cgttcatttc tttactgttg atcaagagat tccaggaaaa gaactagcgg agttacttaa
     2401 aggattcaat atcattattg caagtgttac gccatttttt cgacgagatt tttttgaaaa
     2461 caaagatgaa ctgctattga tttcccgaca tggtattggt tacaacaata ttgatttaaa
     2521 agcagcaaaa gaacaccaga caatcgtatc tatcatacct gctttagttg aacgaaatgc
     2581 tgtagcagaa aataatataa ctaatttgtt gtccatgctt cgacgcacga cacaagcaca
     2641 agcaagagtc aaggacgatc gatgggaaga tcgggcggag ttcgtcggta ggacgctgtt
     2701 taacaaaaca gttggtgtga ttggtgcagg aaataccgga agcggagtaa tcgaaatttt
     2761 gcgtaacggt tttcgctgtg atgttctagc ttatgatcct tacaaatcag atttatacat
     2821 cgaaagcttt ggtgcaaaaa aagtagaact tcaagaatta ctggaacaat cagatatcat
     2881 ttgtttatgt gcgaatttga cagaagagaa ctatcacatg atttctgata aagaagtcga
     2941 tcagatgaaa gaaaatgttt atctatctaa tagtgcaaga ggcgctctag ttgatgaaga
     3001 agcgatgatt aatggtctga aaagcggaaa gatcgcagga tatgccacag atgttttaga
     3061 agaagaaccg ggacgtcaag atcatccgta tttagcgttt gaacaagtag tgattacgcc
     3121 acatatatcg gcctacacaa gggaatgttt agaagcaatg ggtgaaaaat gtgtcagaga
     3181 tgtcgaacag atcagtcagc accttcttcc tgaacgttct gtccagacaa agagcaacta
     3241 tataaaagga tgatgaagaa aatggtgaac ggtttaaaag tgaaaacagg ccctcaattt
     3301 tatttatatg aagaaggtgg tatttcaaaa gtttctgatc tcttgaaatc atacggcgcg
     3361 aaaagagtac tggtcaccca cggaacggta tcatgggaaa aggcgctacc taaattagta
     3421 tttttgaatg acgaaacgat tcaatttttt tatcatcgtt attctggaga atgtagttat
     3481 gctgaagcca gacgaatagc tacaatcatc aaaaaaatga aatcgatttc gtagttggcg
     3541 ttggcggagg aaaactgacc gatctagtgg gttatagtgc caatcttgca agaatcgatt
     3601 tcggagtcat ccctacttta gctagtaatt gtgctccatg gacacctctt gctgtcatgt
     3661 ataaggagaa tggacaagca gaagggaaaa cagaacactt ttacagacaa gctgcttttt
     3721 ttataacaga tccccaatta gtgattgatt cccctgttcc atatttcatt gccgggttag
     3781 cagatacact agcaaaatgg tatgaatctg agctcatctt agaacaagat tttttgcaag
     3841 atgaatcatt tttgaagcta gcacaagata ctgcgaagat ctgcaaagaa gagattttga
     3901 atcattctgc taaagcaatc caagatatgg cacaaagaaa actgacatcg gagttcaaac
     3961 agctttcaga gattatcttt gctgttgctg gtttagttgg aggattagga gataaatacg
     4021 caagaaatgt ggcagcacat gctatgcatg acgctatcag taaatatatt ccggaaagcc
     4081 atcgttttct acatggagaa aaagtagcgt atgggatttt ctatcaactg gcactagaag
     4141 gtcgctggga aaaaatagac gagttatatc ctttgtatcg ggaattagaa ctgccggttt
     4201 ctctagaaca aatgggatta tttccgaaag ataaacagat aatagataaa atggctgaat
     4261 ttattgattc gaaagagaaa gtgcatttga ttcctataga ggtaacacag aaatcactga
     4321 tacagtcaat gatggaactg gaaggttata tcagaaagat ctgaaggagg aggacacatg
     4381 gaaacattaa gtgtagtaca aagtttgttg attgctttat gggtagccgc ggttatgtcc
     4441 cgctggttag gtggaggagc cacgttgacc ttacgttttt ctcctttgat gactggatta
     4501 gtcgtcgggt tgatcatggg ggaagttgca caagcgatga ttgttacagc cgctttgcaa
     4561 atgatttata tgggggtttt ttctccagga ggatcaatgc cagctgaacc atctattgca
     4621 gcagctatcg cagtaccagt cgcattatta ggggatctgt cgccagaagc agcgattgcc
     4681 gttgcggtgc cagtcggatt gctcggaagt tacttgtatc agtttcgttt tttcatcaat
     4741 acttttttag gtaaatatac agacaaagct gtagaagatt tgaatacaaa agctatggcc
     4801 cgatcaatta ttctttatcc aacggttgcc tcatttttat tattcgttcc gcttgttttc
     4861 attgctttat atttaggggc gccattgatc gctgatgtaa tcaaagcttt agagggaaca
     4921 gtcgttatcc acattcttga agtagtaggt ggagggttag cggcaatcgg tatcgcgaca
     4981 actgtatacg ttatcggtcg aaaagattat ctggtctttt tcttccttgc ttattttatg
     5041 agtgttgttt tgaaatcttt agatattacg atggttactt atgccatttt tgggattatt
     5101 atcgctttga tctttgtcca gtcgcaaaaa gggaaacaag tacagcctgc cacaagcaat
     5161 aacaattcag catcttttga tgacgaagat gactatgatg acggatttta atttaggagg
     5221 aatgggaaat ggctgaaata atggataatc aaacaggtgc tcctgaagaa atcacgaaaa
     5281 aagatgtaac aaaagcctat ctgagatggc attttgcaaa cgaaattcct cactcttttg
     5341 aacgttactt ggcgccatca ctactatacg gaatgatgcc aatcttgaaa aaactttata
     5401 aagatgaaga ctctttaaaa gcagcatata aacggcagtt actgttcttc aacactcaat
     5461 taagctgggg tggcggtgtg attactggcc taatgtcttc tatggaacag caaagagcag
     5521 aagaagagta tgaagaaaaa gagatcttga tgcaggatga cttgatgtat aacacgaaag
     5581 cgggattgat gggggcacta gcaggaatcg gagatgcaat cgattcagga accgtccaat
     5641 atatttttat cgcaatcgcg gtaccttggg cacaacaggg aagtccttta ggcgcacttt
     5701 ttccttttat ctgctttggg ttgtatcagg ttttactagg ggtatttttc gcaagacaag
     5761 ctttcactat gggccgtaat gctacaggcc taaagcaaaa cactggtgtt cagacagcga
     5821 ttgaaatgct atcagtttta gggttgttta tgatgggggt gcttgcggga aactatgtaa
     5881 aagtagaatc ttcattgaaa tttgcgatat ccggacgtga gtttgttatc caggaaattc
     5941 ttgatcaaat cgtgccaggg atattacctt tagctgtcgt aatgggtgtg tacttgttct
     6001 atacgaaaaa aggattgaaa gtcacacaag cattattatg gctgactgga atattaattg
     6061 tgctagcagg aatcggtatt ttataaaaag aagggaacgt gaataagatg ggtgtagtca
     6121 atttagcgcg tgtggatgag cgtttgatcc atgggcaagt aatggtaacg ttatctcaaa
     6181 aaagtggagt gaattccatt tttgttgtag acgaagttgt agcaaaagat aaatttatga
     6241 gagatcttta caaaaatgca ggaagccgta caggacaaaa aacaattgtc attacaccag
     6301 agaaagctaa attctattgg gatgaatatc aatttaaaga atacaattgt attttgatcg
     6361 caaaaacagt ccaaacgatt tacgacttag taaaacacgg aatccccatg gaagaattga
     6421 atattggagg gattgctcaa aaaaatcctg atacagatct tttagtgacg aaatctgttt
     6481 atttaaataa atcagatgct gaaaaattga aagaaatgaa tgaacaatat ggcgtaaaga
     6541 atatttattt tcaagccact ccatctgcac ctaaaacagg attaaaagat gtattgaaca
     6601 agtttgattt gtcataaaca aatgcttaga aaaaagataa agagggcgaa aaaatgaaga
     6661 agaacgagct gtttcgtgat tgggaatttc gttatcgcta tatttaccgt aagcgccgta
     6721 caaaaaagag taaacagcgt tttctttcag cattagtatc agatatttac agtatgagaa
     6781 cagatgtgac agtcattgct tacgatacgc tagcctaccg atcaaaaaat atttatgttg
     6841 gagatatcga aaaagcagag aaagttatct gtacttatta cgatacaccc gttcatgcat
     6901 taggaagtta tttcatgttt gattggaaag accaaaggaa aaaaacaatc tactctatat
     6961 tattgtcctt cattttgttg ttcagtttag gttggtgggg aatgatgata tataacaaaa
     7021 atcctcatca cgtattcgat ctgctatccg tataaacagg tatcactgta ctagcatttg
     7081 ggagctactt tttcttattg ggaaaagcag ctcgcggatg gcctagcaga caaactttta
     7141 ttcgaaatac ttcatccata ttaacgatgc tagaaatgat ccgtacgatt gatgatccga
     7201 gtgtagcgta tgcgtttgtt gatgaaggct gttatggaga aaagggctta gacagcgtga
     7261 gatcgggtat gaaaaaagag gctattcttt tttatctaga ctctgttggg gcagatacac
     7321 cgctacagtt ttcgggaaat tatttttcaa ataaagaaca gtggctgaaa caagttgata
     7381 agttgaaaga aaaaaacgtc aattatattt ttagtgcaag aaaaaagcaa gcgcagttct
     7441 tctatttgac caaaacagat ttacgaggga aaacatttaa ttggcaaaat gccaatcaga
     7501 ttattgctct ttttcgataa gtaaaaagtg caaaacaagg agtgagaaga ctaatgttgg
     7561 aaatcatgat cgttacacat ggtaagttga gcgaaggact gaaagatgcg gcggaagtaa
     7621 ttgtcgggac atcgagccat attgaaacaa tgtgcttaga gtctggggag gacatccaaa
     7681 cattcggaga gaaggtcaaa gaaaaattaa ctatgatcaa cacagcagaa cccatgctag
     7741 tatttgttga tctaattagc gcaagtccgt acaatcaagc gctcttggca gtcaatgaac
     7801 tgactgaaga aaagaaagca gccacttatg tcataggggg cgtgaatctg ccaatgttgt
     7861 tagaagcaat caatcatcag atgcttcaca caccgataga agaagcggtg gaacaaattg
     7921 tcaaacaagg aaaagataat ttaactgttt ggcatgcttc agcggaagaa aaaaaattag
     7981 atgaggacga tttttagaag gaaggctgaa aagatgaagt ggggatttag atggtatggt
     8041 gaaaaagggg attcgatccc attaaaatat gttcgccaaa taccgggaat aaatgggata
     8101 attggaactt tgctgactaa aatgcctgga gatgtttggg agatttcaga aattgaggag
     8161 ttgaaagcat ctgttgagag ccaaggactg gaactgcttg gaattgaaag tgtagcgatc
     8221 cacgatgcaa tcaaggcagg gacagatgaa cgagattact atattgataa ctatatacga
     8281 acaatcaaaa acttgtctcg atgtggggtt cgattgattt gttatagctt caaaccaatt
     8341 tttggatggg cgaaaactga tttgtttaat gaaaacaacg atggaagcta ttctctttta
     8401 tacgatcaga aaatcgtcga tcagatggag ccaggagaaa tgtatcaatt gatacacagc
     8461 cagtcaaagg gattcaaatt gccgggttgg gaagaagaac ggttgaaaaa attcgctcag
     8521 ctggaaaaac tgtatgaagg aatgaccgaa gaagaattat ttgaaaatct gacttatttt
     8581 ttagaaaaag tgattccagt gtgcgaaaaa gcagatgtaa aaatggctat ccatccagat
     8641 gatccgccat gggaactttt tggttttcct agagtgacga aaaatcttgg cgacttgaag
     8701 aaaattctaa atgcagtaga ttctccatat aatggactaa cgctgtgcac aggatcttta
     8761 ggggcaaatc cggaaaacga tttagttgaa atcatccatg aggtaggttc gcgcatccat
     8821 tttgtccact tcagaaacgt ggagttttta ggagaaagaa agttcaaaga aacggcacat
     8881 ccctctacag aaggctccct tgatatgtat catattatga aagcgttggt gcaagaaggg
     8941 ttcgacggtc ctatacgccc tgatcatggt agaaccgtgt ggggtgaagt ggctatgcct
     9001 ggatatggcc tgtatgatcg tgcaatggga ctcacttaca tgcaaggtct atatgaggcg
     9061 ataagtaagg aaaaaccact gggagagcat tatgaataaa atcaagctct tcatctttga
     9121 tatggatggc cttttattag aaactggacg tttagcttat cgtgcttatg taaaatcagc
     9181 acaaaagtat gattatgaaa tgcgtaagga agtctattat ttactaactg gccaaacaga
     9241 aatggcgata agagaacaaa tgggcatttt gtacggagaa gatgttccat atatcaaatg
     9301 gcgagaagca atcaatcaat ataaagaaaa aatcgtgaaa gaagataaga gagtatatac
     9361 gaaaaaagga gcagaagaaa ttttatcttt tgcaaaagaa agaggaatcc atacaatcgt
     9421 ggcttcgtct aatacacggg taaaagtaga aatgtattta aaaatggaaa atctatatgc
     9481 cttgtttgat caaatcattt caggggatga tgtaaaaaaa ggaaagccgg aaccagagat
     9541 ctttttgaaa gcttgttcaa aaatgaatat tcctccaagt gaagcacttg tatttgaaga
     9601 ctcgattgca gggatcgaag cagcaaggcg agcgggcatt ttgtcttttc tcatcaaaga
     9661 tcatttgata gggttgcccg atcataatgg gaagcataag ttgaaagtag atgtaagttt
     9721 atatgaatcc aataaagcag actatgaatt ttatgacctg catcaagcaa aacgattttt
     9781 agaaagtaaa gggttgtatc tctaataaag aggactattt tctagtcctc tttatttatt
     9841 gtcctgcacg actgagggaa cagatgaaaa agaagtaatt ttttattttg aagtatctac
     9901 cttatttgta gtgaacaatt tagaacaaac acaaaacgca tctactgtt
//
LOCUS       DAEGIQ010000096         9902 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000096 DAEGIQ010000000
VERSION     DAEGIQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.098"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            264..470
                     /locus_tag="KMT45_002210"
     CDS             264..470
                     /locus_tag="KMT45_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBE7005661.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(672..1673)
                     /locus_tag="KMT45_002211"
     CDS             complement(672..1673)
                     /locus_tag="KMT45_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE7005662.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1678..3591)
                     /locus_tag="KMT45_002212"
     CDS             complement(1678..3591)
                     /locus_tag="KMT45_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBE7005663.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3759..4265)
                     /locus_tag="KMT45_002213"
     CDS             complement(3759..4265)
                     /locus_tag="KMT45_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBE7005664.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4425..4865
                     /locus_tag="KMT45_002214"
     CDS             4425..4865
                     /locus_tag="KMT45_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBE7005665.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4891..6048)
                     /locus_tag="KMT45_002215"
     CDS             complement(4891..6048)
                     /locus_tag="KMT45_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005666.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6051..6407)
                     /locus_tag="KMT45_002216"
     CDS             complement(6051..6407)
                     /locus_tag="KMT45_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005667.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6705..7679)
                     /gene="sgrA"
                     /locus_tag="KMT45_002217"
     CDS             complement(6705..7679)
                     /gene="sgrA"
                     /locus_tag="KMT45_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBE7005668.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7875..8714)
                     /locus_tag="KMT45_002218"
     CDS             complement(7875..8714)
                     /locus_tag="KMT45_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBE7005669.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8899..9786
                     /locus_tag="KMT45_002219"
     CDS             8899..9786
                     /locus_tag="KMT45_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE7005670.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         2971 a         1984 c         1495 g         3452 t
ORIGIN      
        1 taggatacat cgaatttttg gggaaattct tttgggggta tcctaaatct tttctattat
       61 attcccatat tttctattat tggtctagac gtatgatggt aaactttgta cctatctgat
      121 tgctgaatca ttaattgctt atcattagcc atctttgttt caagttcgct agatgatcat
      181 tttattcaca tcctaacaaa cttgtcacat ttatagattc ttaatctatt tatcatacaa
      241 ttataaaaaa ggaggtgctg cttatgaaag ctatccatat ttctttgctt tttttcgcta
      301 gtgcattgat tttatttgct attcatcttt gttcccagcc ttatatcaat aacaacggaa
      361 tattagtgga accagcattc ttttgcttac cactcggctt tttgtccctt cttgcctccg
      421 caggcttcgg atttgttgct ttttttaaac gatcaaaaca acaaatctga cttataaaat
      481 ttacgtaaaa gagatcgtga caataatcgt gtgatattcc caaatttcga ataaaaggtg
      541 ccccttgcca tctctaactc ctcagtcaca agttacaata ttcataaacc atgaaaagct
      601 tgtttattga aattgctcct tgtgacttgg agctaagtga cagattacaa cctcttttgc
      661 atcataagac tttactcctt gaaaaacatt actaactttt gatatgtttc ttgcgcttgt
      721 ttattgctga aatcaaactg atattcatgg ttcaaaacag catttttctc cgcataatct
      781 acttttgtga ctttcacccc cgctttctca aatacagaag ttacttcttc catttgtttc
      841 gtaaacgtat ttgtatttcc atccgttaag aatgtgtttt gcgtccaacg atccgcatgt
      901 ctgaccaagg ctaaactttt gaccttctct gattgttcaa agtccttatt atcaaagtaa
      961 gcccatccac atttttcata aagaaagcta gagattttat cacttacttg agtaagtgca
     1021 gaaggatcca acagaccaga caaagaaata aacttcgtaa tctgcttgtt ctccaaagtc
     1081 ggatcaagat gatcagcttt tttaatttct tcattttgaa gtgctaacaa atattctcca
     1141 ctaatttgtg cacctgccga atttccacca atcgaaatat tcgaccaatc tgcttgatct
     1201 tttatctctt ttttagctgc ttgaatggct tcgttcaatt ggataactgg tgtaggatgt
     1261 tttgcttccg gtgatcgttc ataatctata tttacgatga tttgctgcgt gtctgaacat
     1321 agatattcca aataatcacg acaatcattt ttatcccctc ctacatatgc gcctccatgc
     1381 atccagaaaa gaatcggtaa ttttttcttt tccactttcg gtgtgtagat atccatatta
     1441 ttatttttgt actttgaagg ataggatatt tcttttttta tatcgatttg ttcgattttt
     1501 tgctggaaat catctggtcg ggcatatact tgttcttttt cacttgtgtc aaattgtttt
     1561 ctaacaagca agctaatcgg ctttggtgtg atttgattaa tcaaaaatac agtgactaaa
     1621 ataactacga tgataactgg tactgcaatc actattcgct tgaaccattt cattcattta
     1681 ccctctcttt cttcgccaaa tccatgtgat aatcgtgcta acagataaaa atccacctga
     1741 ataaatgaga agcgaatgtt tcttttctcc agttttcggc aacattcttt tttttgcatc
     1801 tgtctttttt tgtactttca ctgtcatctg aatcggttgc gtatcatcac ttgattgatc
     1861 ctctcgaggt tcattctttc ccttattgtc tgcattctct gtcggatctt gcttttttgg
     1921 tggagctact gggatttttt tgtaatagaa aataatagtt ggtatcgtct cattaaaaat
     1981 cccttcagtc ggtccttcaa catttgctaa ttcgtatcct tcgaatgtct tttgctctgt
     2041 catataattt tctccataag ctccgtaaag ctggatttcg tcctggtctt tgagtagact
     2101 tttattgttt tcatccacat actttacgtt tatctgatca tggaacacta ctttcaaaaa
     2161 gtccgttaca tgtttcgtgt aggcagcatg gtcaacttcc tgtgcctttc catgccctgc
     2221 tccttctact aataaaagat ctttataccc tcctgataac tcatataatt tttgcgcgtt
     2281 agacacaggt actacatcat ctgctgttcc atggataatc aatttaggca atgaatctga
     2341 cattccaatc gtaggtagag cagcttttgt atccaaatgc atctcctgat caaaatagta
     2401 ttgtttcaaa aggcccataa ataaatcttc atgctcttga tccccaatga tgtctaactg
     2461 attttttatg gcatccggta gtgcgggggc tactgtctgg ctgtacgtaa aacgtagttg
     2521 ttcatcaatt gatgcaaatc cgcaattttc aataattcct ttgacattga caggaatatc
     2581 tttcgctaat acagacatca tcgtagctgc tcccatcgaa ccaccataaa gtaaaatctt
     2641 ttgagatggc cgctctcgaa cttcttgatt tatccagtaa aggacgtcat ctgactcata
     2701 ttgtccatag gtcacataat cgcctccgct attaccagtc gctctggaat caacaaataa
     2761 taaattatac ccggccttat aaaaatcatt gtattcttct gtaacgatcc cgttattcca
     2821 attccctcga aatccgccat ggatcatgac tgttttatct gaaccttgat cgacataata
     2881 tgcatataca tccatcattt tgccatcttg tttgtttagt acagtttgat gaagttgttt
     2941 tgtatttgga tagaagcctg tgttttctgt aggcgttgtc ccatcatctg tacctccaag
     3001 catcgacatc aacacactgt tttttgcaaa agcgatatcg tacaatagat aagtagcaat
     3061 cagatgtttg ataatctctt tatctacacc ataatcatta acagcaagct cagtgactaa
     3121 taactctatt aattttgact tgatgtcttc tgttggtcca ttaagtattt ctgtcatctg
     3181 tttaccgaag taattcaaat attctttacc attgaaattt tcacctttac cgatatcatc
     3241 attttctgtc ccattctctt tgtcacttac tttagcagaa gaggattcgt cgtttgacat
     3301 ttttgtagct gttgcttctt ctgtttgttg gttatctgtg gtagcggtcg tttcttgatc
     3361 tgtgctagta acagatgtct caaccgttgt ttcagtattt gtttctgttt gtgaagtact
     3421 cgatttcgtt tcatttccag catcagtagt ttctgtggtt tctacctccc gagattccga
     3481 ttcggaagaa atgctctctc catatactgt ccattgcggg acgcttgtac ttacagcaaa
     3541 taagatgacc aacgctccct ttaatacttg ttttgttctc aagctattca ctccttttta
     3601 tgtttctagg agaattatat gtaaacgcaa acatttttta tacaaaaaat aaatggttta
     3661 tcccataaaa atagaacagg attattttct cattttacgt aataaagcag aatggataaa
     3721 ccagtatcca ttctgctttt tttaaacgaa gaaacaactt attgctccaa aaatgtcaga
     3781 aatcgtttat cccaaatttt ctcgtaaaaa gcgattgttt cctttgcttt cataaaagga
     3841 ttatcataag ttgtcgttgc tcctctacac atcataagct ggtatcctaa tccatgagca
     3901 aacttcacag tcgtatctat acaatactct gcttgtgccc cacataattc gatcgaatga
     3961 cagtccaatt cagttaataa aacactcaaa ttagtcttat aaaatgaatt cgcatgggtt
     4021 tttctcacaa aataactgtg ctcaggaata gataattccg acaatagctc ccatggtttt
     4081 gtttgtggca ctaattgagc atcttcgtgt tgaacgaaaa taactggttt cccaatcgtt
     4141 tcgtatgttt tgattctttt attcactaat gctgtcactg tttctaattc gtgtatcggc
     4201 gaagaatcaa aacatacccc tttttgtaaa tcgatcacta ctattgcgtc ggctgtgttt
     4261 gacatacttg tgccttcttt cttttttctt gatttaatag tatttcttat ctagctagtt
     4321 ccattatacg tgacaacatt ttcttatttt acacctctaa atggcacttt tcgactattt
     4381 cttcatgtta ttatttcata aatacttagc tattggagga atgtatgatg attaaattgt
     4441 atacaggttc tggctgctcg tcttcaagac gcgcaaaacg ttggcttatc gtccatgata
     4501 taccgtttat cgaacagaac ttaattcgtc aaccactaac caaaaatgaa tttatccact
     4561 tactaaaatt gacaaacaac ggcgtgaccg aaatgctctc tactcattct attagttata
     4621 aaaatcttgt tcacagaata gatgaactca gtttgagtga actatatatt gagattcgta
     4681 catccccttc tttattgaaa aaaccgatta tctacgacca atctgcaaaa cttttgactg
     4741 gatttagcga agataaaatc aggatgttcc tttctaaaac taaaagaaag caagagctca
     4801 acgaactctt gctttctggt catcttgtcg attttaaaga aaataatctt tttgcaatgt
     4861 cttaattttg gcataaacaa tctgcttttc ttaattaacg aaatccaatt tttccaatat
     4921 ggatctattt ttctcattcc tgatttttat gatttcagct ttcagacgcg cccaatcatt
     4981 ttttgtaggt tgtccattcc agtaaaatat ctgtgtattc gtttgatcat atgccttttt
     5041 gtcgatagga taatcgctaa tgacaatatc aggtaactga tcagcggatg cactatattt
     5101 tattgggcgt tcagtcatag ataatatcct tttctttgcc cataattcct gtgttttacc
     5161 atactttgat tgaatataga atatgacagg ttgttcgtgt tttataaaaa tatccctgac
     5221 taacatacag tattgaaaaa tcaaccgtct gttcttttca taataagtat taagctctgg
     5281 aattctgcga gctaccccac taaaaaactg ttgcatgatt ggatacatca ctggaaatat
     5341 atgctgaaaa tctctgtctt ctgctgattt tccaaaagca tccaccttct tttttgtccc
     5401 aaaaaccctt gaatacaaat ggatcattgc caaattgaca tatagataat ttttttctga
     5461 agttgtaaaa caaatcttga agtttttttc aaaattttct acaatagacg attcaatcca
     5521 gccccaactt tcttgtactt tatctggagt atccaacaaa tcttcttcca aataggtgtt
     5581 cattacacca aacataaaat aaaggtaagc ttgctctgtt ggagtgatgt gattcatcga
     5641 ccagaagttt gcttcaaaaa aaggctctag tatgtaatgc gtaaactctt cttttgttat
     5701 caatggatgc acaaatttgt tcatctcttc tgggacatac gtaatcgagt ggccttgttt
     5761 cattcgttgc aacgaaattc ttagtaggct gatccatttc tttctatcta tactacgcgt
     5821 atatggcgca tgtttcgcaa atttttctaa tagttgacgt ttatttttta tacttgataa
     5881 tatatcagaa tgattttcgt cgaatgactg ccaatacatc aagtgaaaaa agtatcggat
     5941 acggtattct tctccttcta tcggattacc ttttttcaag ctgcaagata aatgatagtt
     6001 tttcaaaata gtattaagtt ctcttaattt tctttgaatc gtacgcaaac ttatataatt
     6061 ttttgactaa gattttctaa ggattgataa gtacctttgt aaagtgaatc cagtaaaata
     6121 taaccaatcg ttccctctaa aagtatagaa taaactttac tcagtaaaaa ctgagaagaa
     6181 aactcgactt ttatcatctt gttttctgaa agaaaagata atttatattc tggatacctt
     6241 tcgcaaaacg attctgcttg ttgaatcacc gaattcatct tagccagggt gtagtttaga
     6301 tctaaagcga tatccttcac cacatataat ttctcgtctc tttcataaca atagttcaaa
     6361 aattgatata cttgcctttc attgatatca ataaattctt caaacaaagt acatactcct
     6421 ttttatctat tatttagact tattccgaca gctacatgaa taacgtacaa atagattcgt
     6481 tactgatttt tcagagatac taccatgcgc agtgtgcatt gtcaattatt cttgctctta
     6541 ttttccattt gtgttcctgt tatttcctgt ttctcaaata tgttagtaaa caaaaataga
     6601 tctggcaaag ctacaacttc gccaggcaat acagcactct cgaatgaaat attcactcaa
     6661 aaatctatcg ttttattcaa ttttagatct gttgctttta ttctttattt cactcgatgg
     6721 aagagagaac gaaaatagct tgctcctaca atcaaaatga gtaacacacc catccatgta
     6781 attagcttgt tcaaggttct actaccagtt tctggtaaat cgctttttct attatctgtt
     6841 tctttcgctt tttcttgatg atcaattcca cgagaaacaa aatcaggctg ttttagcaca
     6901 ctttttaggt tgctccctgc atttctcttt tgttccttag ttggtatgat aggtaccaaa
     6961 tcaagttcat tatttacttt tcctgtgctg ctttcgctgc tcgtgcttcc tgtgctactc
     7021 tcactactca tgcttcctgt gctactttca ctactcgtac tctccgtact gctttcgctg
     7081 ctcgtgcttc ctgtgctact ttcactactc gtactctccg tactgctttc gctgctcgtg
     7141 ttttctgtgc tactttcact gctcgtactc tctgtactgc tttcaccgct cgtgttttct
     7201 gtactgcttt cactacttgt gctatccgta ctgcttggcg gtccaacaac tgtccaactg
     7261 cttggtttca aattctccgt gatcgtcatc tgtccatcat aaaaaacaat caaatcaact
     7321 ttatctttac tcttgatctc atcaatcgat agttctgcag aaatcattat tgtatactca
     7381 ataccagcgg tccattgtac gtcttttaaa taaacttccc caataacttt ttctgaactt
     7441 ccattaccat cccaaccata tgttagaaca gcatgtacat cactagcatt cacgcttttg
     7501 tttggtttga ctggaatagt aatgacttgt tgtgtcatgt cataaacaat ccgatcagtt
     7561 ttcacagtaa atgaaagatt ctcatttgcc cgttcatttg cagaagtact gattccaatc
     7621 attgctgcaa ttatactcag cccaagaata acaaataata cggtcgctgt tttcttcatt
     7681 tctatcactc tccatataat taccttctat ttgataaagg aattatataa aatgaaaatg
     7741 atgagaacac gacagtcagt tgtgaaaatc taacaaaaga tgtcgtgtat tcgcttgaat
     7801 ttgtttactt tttcgtttaa tcaatgacag acttaaaaaa gcaagaccga tatcagcctt
     7861 gctttcttca tcttttattg ataaacagca gtgtatttct tcatccgctt tgttttatat
     7921 gcatagagta acaaaccaac aatcaagact aacaagaata atgtagttga tatagatgct
     7981 tcagccccaa agtttccccc agtaattatt gtattttgcg attggatcag agtggagaaa
     8041 aaaccttgtt cttgcgttcc gctgactggt atacccaaaa tagctccttg tgtccgattc
     8101 caagtaatat gagcaccaat agccgtaaat aaacttcctg tactgtagat aagtgcgctg
     8161 aaaaagcaac ctgctaaaaa gatattgaaa actgccagaa atgtcatgtt aggattcaat
     8221 ccgtgcaaaa gagcaaataa tgcagcagat ccaatcattg ctattaacgg accaaatcta
     8281 agcatctgat gctgaaaaaa gccacggaat aaaagctctt ctgatgcccc cactgcaata
     8341 aataaaataa aactcgttat cagcggcaca atgtttgcgt ttcctgaatc taaccgatac
     8401 gcccccgtca aatataatgg actagtaata agcagaaaca ctccaattcc aacaacgaga
     8461 cctcccgcta aataccccca gtcttttcct cgaacagaaa caccataatc ttctattctt
     8521 tttccttcga ccttactagc aagaaataaa gctagtaaaa aaacagctaa atgtccgacc
     8581 gtaccggcaa ttgctccaat ccattcacta tttccaataa acatatccgc aagtgcatta
     8641 ttgacaagaa ttcctaaaac aaaaccaact aagactatta aaacagatcc aatcaaccga
     8701 ataatcctga tcatctttca tccctccttt atattaagtt taacaaccct tttctttctt
     8761 gtctcgtgaa aactatgtaa atgttctcca tcatagagag aatagatttt tacgaactaa
     8821 gaatatctac agcaacctta cgaaaatgaa agactgtctc cctaattctt ctgttagact
     8881 gtttttaata aggaaaggat gggaaatttt ttgaaaaaac aactactaat tatgtgcctt
     8941 tcagcatgtg caattttact attctcaggt tgtagcaagt ccaatgaaac gattgcaacg
     9001 tttaaaggtg gaacacttac agttcaggat ttttatgata acccaaaagt taaatcaaat
     9061 aatcaaacac tattaaaaaa aatgattgtc ttcaaagcat ttgatgatgt atacggaaaa
     9121 gagatttctt caaaggaagt aacaaaagaa tataatgaac aaatcaaaaa actcggtcca
     9181 aactacaagg aacaattaaa agcagttggc caaacagaag agacatataa gaaactcttt
     9241 aaacagatgc tggctttcca atacggtttg aaagccaatg taaaattaac agataaagat
     9301 ctagacactg cttggaaaga attctatcca gaagttagta cacagatcat tctattttcg
     9361 actaaagaag gagcagataa agcaaaaaaa gaagcaaatg aaggagaaaa tttctccaag
     9421 cttgttcaag cttatggaaa aaataaaaca ttaaaggaaa ctgatggaaa aatgaatttt
     9481 gattcaacca atccagagat tcctactgaa gtaaaaaaag cagcatttaa actaaaaaac
     9541 ggggaagttt ctgatatcat tcctgtaaca gatccaacga cttatcagca aagctattat
     9601 ctagtaaaaa tggtaaaaaa acaggataaa ggttctaata aagacaaata caaatctgag
     9661 ctagaaaaaa ttgctaccga agcaaaaact cttgatacgg aattcatgga taaaacaatc
     9721 cgtaaagtga tgaaaaaaga caatgtaact attaaagatc cttatgtaaa aaatatattc
     9781 aaataaacaa atttgaccca attggggatg tggcaaaatt tgagaagtga tttttggaaa
     9841 ttccttctta tttttgaggc aaaccacttt tgccacaact tcatcggggc ctttttttat
     9901 tt
//
LOCUS       DAEGIQ010000097         9681 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000097 DAEGIQ010000000
VERSION     DAEGIQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.099"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            51..260
                     /locus_tag="KMT45_002220"
     CDS             51..260
                     /locus_tag="KMT45_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005671.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="KMT45_002221"
                     /pseudo
     CDS             467..667
                     /locus_tag="KMT45_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="KMT45_002222"
     CDS             complement(1379..1984)
                     /locus_tag="KMT45_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7005672.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="KMT45_002223"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="KMT45_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="KMT45_002224"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="KMT45_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="KMT45_002225"
     CDS             3019..3990
                     /locus_tag="KMT45_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7005673.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="KMT45_002226"
     CDS             4130..5443
                     /locus_tag="KMT45_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBE7005674.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="KMT45_002227"
     CDS             5486..6352
                     /locus_tag="KMT45_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7005675.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="KMT45_002228"
     CDS             6349..7197
                     /locus_tag="KMT45_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7005676.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="KMT45_002229"
     CDS             7211..8683
                     /locus_tag="KMT45_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBE7005677.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="KMT45_002230"
     CDS             8700..9572
                     /locus_tag="KMT45_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBE7005678.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         3037 a         1659 c         2019 g         2966 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa g
//
LOCUS       DAEGIQ010000098         9292 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000098 DAEGIQ010000000
VERSION     DAEGIQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.100"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(416..1123)
                     /locus_tag="KMT45_002231"
     CDS             complement(416..1123)
                     /locus_tag="KMT45_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005679.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1116..1553)
                     /locus_tag="KMT45_002232"
     CDS             complement(1116..1553)
                     /locus_tag="KMT45_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005680.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1568..1819)
                     /locus_tag="KMT45_002233"
     CDS             complement(1568..1819)
                     /locus_tag="KMT45_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005681.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(1819..2025)
                     /locus_tag="KMT45_002234"
     CDS             complement(1819..2025)
                     /locus_tag="KMT45_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBE7005682.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2462..2722)
                     /locus_tag="KMT45_002235"
     CDS             complement(2462..2722)
                     /locus_tag="KMT45_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005683.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2797..3000
                     /locus_tag="KMT45_002236"
     CDS             2797..3000
                     /locus_tag="KMT45_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBE7005684.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3141..3626)
                     /gene="ssb"
                     /locus_tag="KMT45_002237"
     CDS             complement(3141..3626)
                     /gene="ssb"
                     /locus_tag="KMT45_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE7005685.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3629..4528)
                     /locus_tag="KMT45_002238"
     CDS             complement(3629..4528)
                     /locus_tag="KMT45_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBE7005686.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4541..5134)
                     /locus_tag="KMT45_002239"
     CDS             complement(4541..5134)
                     /locus_tag="KMT45_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBE7005687.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5210..5359)
                     /locus_tag="KMT45_002240"
     CDS             complement(5210..5359)
                     /locus_tag="KMT45_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005688.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5375..7579)
                     /gene="topB"
                     /locus_tag="KMT45_002241"
     CDS             complement(5375..7579)
                     /gene="topB"
                     /locus_tag="KMT45_002241"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBE7005689.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7590..9035)
                     /locus_tag="KMT45_002242"
     CDS             complement(7590..9035)
                     /locus_tag="KMT45_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBE7005690.1"
                     /translation="MSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFVFDRRK
                     QRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVENLHFET
                     EKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNFLVRDD
                     RLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFNLKIGD
                     PKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRKKQAFP
                     QSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKIIYEEVA
                     KEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKDICREV
                     AKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPEVKKDW
                     NDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9110..>9292)
                     /locus_tag="KMT45_002243"
     CDS             complement(9110..>9292)
                     /locus_tag="KMT45_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005691.1"
                     /translation="QHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKR
                     KNEIYYEQQLSQKERV"
BASE COUNT         2371 a         1908 c         1587 g         3426 t
ORIGIN      
        1 acgggtaacc gtttatttcc tattgcaatt ggcattagta ctctctcctt tcatcgaaat
       61 aaccctatta aaaatatggc tgcaatcaac ttgaatatta gactcacaag ggttaacaca
      121 aagcgccaaa agatcaaaca aaataccagt attcctaaga atactacgaa gtttaaatgt
      181 agagctgcac catgacgcat aagtatctcg ccaacaaaca gtacgaacaa tagaggaatg
      241 attttattca tatggatctc cttctttctc ttaggttatc gggctttgcc cgcttatttc
      301 gcaccaccta agcggcgggg gcggttggat cgattggtgc gaaataagaa ccattccctt
      361 aatttcaagg ttttttgttc ttatttcaca catcctcact tttcggtata aatggttatt
      421 gacctttttt cctttcaaat cgttgatatg gcattcgttt acggtcaagt tcattttgga
      481 tttcttctgt atcgtcgtaa gtgtaggtgt ctaaaaaatc agttaaagtc gtgtaccagt
      541 cgtcgcctgg gttatctact tcttctacat attccctcaa aaatgcttcg ggtacagtga
      601 gttcataatc cattcccatt aacacgatag attccgtccc acacgtcaaa aatctagtaa
      661 atacaaggta ataatcgtct accgtcgcat ctttgctgtt tagctcttcc atttcttctt
      721 ccattaattc catgctctca aaaattgaat aatattctga ttctgagtaa tagtgtttcc
      781 tgcaaatcgt gtagctgaca aagttttcga ccagctcact cctttctagg taaacattca
      841 tgatatattt ttctgtttct agtcgtagaa gctccgctcc tttttttaat gtgtcaggac
      901 gacgtttata cacgtctaaa cgattatggc tgactgtaac cttcgtttga ctcgtttcac
      961 tttccatata caactgttct tccactcttt gaataaagta cttcatttct tccatacaac
     1021 taaagtcttt ttgaatccgt ctgattactt cattcaagtg ttctttgttt gttaaaaatt
     1081 gcgaaacatc ttctgttgtt agctgataaa ccaccttatt cattatcttt ctcctccgat
     1141 tcaatcgtat aaaatgcacc atttttttcg atattttcaa aagtatctag ccagtcgcta
     1201 gccattgctt tagctttctt aatctgtgcg aattttgcat aagtcagttc taaggcttga
     1261 tattcggaag gtttgatagt gcttggtttg acacctactt ccaagcatag taaggcttgg
     1321 aaaaaccatt ttccattttg ttttttcagt tcttccagtg ttcgatagta cgtcgcaatg
     1381 agattcattt cgacacctga ttcaatcatt tcttccactg cgactgacaa tttttccttg
     1441 taatgttttt tgggtacacg tgtttcatgg tataggaacg tcatatcctc cattttttgt
     1501 gccattttat ccaaaggtaa tttgacaaat tcgtcatagt aattttgagc catatttttt
     1561 ctccttttta gttctctaat ttggttaacc aatattcgat ttcataagtt tccatgtttt
     1621 cttgataatg cagttcatat cctgcaatct tcactaggtc ttcaaatagc atagtcggtg
     1681 catcctctac taaaggggtt tctatcgtcc agtaccgcgg aataagtaaa gcttctgcta
     1741 ctttttcaga aatgacttct ttgtccacca aaatttgaac gacttctttt acagtcaata
     1801 atttgtgttg tgttttcatc accgtttctt tactttctca tcaatcaaaa cataatcgcc
     1861 ataagacaag gtatattttt tcccgcatgt tggacattga tacaaacgat agcttttgat
     1921 acatttcgct ctttctccac aatcgcattc gacagtatgt ggtgcatgat agtttgggaa
     1981 taattcgctg aagggaacac cccaataatc aaatttcttt cgcatgattg attctgcctc
     2041 ctcttaggaa aggttttatt tcctttcgtt agttctttct ttattgattt gttggccaga
     2101 atgaatcaac gaaaagcgac aaggacaagg gcgcgcagcg tgccatttac ccttgtcctt
     2161 gtcgcttttt ggtgataaaa ggcaacttgt tgccggtctg gcaaatcaat aaagagatag
     2221 cgaacaggaa cgaacctttt ggtgagagga agttttttct attttgtctt gctttgcaag
     2281 acaccatatc ttagtgaacg tgcaacgttc acttcctttc ttctcgaacg gcggtggcgg
     2341 ttggagtgag agaaaggttt ttcaattgag aaggcacttg tgtcttccta aaaaggtctt
     2401 ttcctgtttc tatgtaaaac aaaaagagct gcaatcctca tttaggactg cagctcttct
     2461 tttatatgcc catcgatcgc gtaaaatgat cggggtttct aatttgcatc gattcaatga
     2521 ctaattgatt ccgtttattg aaatgacctg tgacggcaat attgtatttt ccatcatcca
     2581 taattaacaa tttatcgata atttcaatat ctgcaactac acaattaatc gattcatgaa
     2641 ttgtttgtaa agtaaagcga accaataatg gttgtgtttt tattactcgg attttatcaa
     2701 gcaaaccaac atatttgttc atcacgatca cctcgctaaa attatagcaa aaacagctaa
     2761 aattgagaat ttttattcga ttttagatat gctataatga tcaagacagt aaagtttgtg
     2821 acggtggcta tccgattgaa agtgaggtgg tgcgtatgtg cgtacttcca aaatcttaca
     2881 gaaaggagaa gccttttgcc tgttgaaacg gcgctaggat taatgattag tttcggttct
     2941 ttgatcgcaa caatcatttt tggaatctta gcagttgtaa acaacaatca aaaaaaataa
     3001 ccgtcagctt tagccggtcg acgattattt ttcaaaaata atattaagct ggtcaccgtc
     3061 ttaaacggtg ctgttagggg agttgttagc gcaactcctc ttttcgtatc cattataaca
     3121 actttattga tcttttacaa tcagaatggt aaatcatcat cactaatatc gattgattgt
     3181 ccgccaaatg gattttgtga agcgttcaat ccattgttgt taggcgtagc tacatcccgt
     3241 tgttcaaagg tggtagggct tggactgctg gacgattgat cagcgtgcgc acggctttct
     3301 gtcgctgctt tactttctaa tagttggaaa ttatcggcca caacttctgt cacatacaca
     3361 cgttgtcctt gttggttatc gtaggttcgg gtttgaatcc gtccaacaac gccgatcaaa
     3421 ctgccctttt tagctaatgt cgccatcgtt tctgctggtt tgcgccaaat cacacagtta
     3481 ataaaatcag cttctcgatt gccgttttga ttcgtgaaat ttcggttgac agctagggta
     3541 aaagtcgcta ctgcagtacc actcgcggta taacgtaaat ctggatcttt ggttaagcgt
     3601 cctactaaag ttacattatt gatcatcttt atctctccat ttcttgattc atttgttgtg
     3661 atcttttaaa ctgccgtttt ctttcttgat tttcaatttt gatcacactt ttcattaggt
     3721 gatcaatttc gttgactgta tctgtaaatg aaatggaatt cgcaaacgtg ttaactgagc
     3781 gatatttttc ccactgcttt tccaaatagt catttcctct gatcttatcc atttctcttt
     3841 cataataaaa ggacaacggt tttgtatccc cacgttttgt aaatgtattt cgaacacttt
     3901 tgtataactc tttcgtatcg atattttcta gtttcggaat ggtaaataag tcgtagtaat
     3961 cttttgctct cgaattatct gtgccaaagc tgaacgtagc acttagcttt tctgcaataa
     4021 tttgttctgt tggataagct ggaatctcca cttttttatc ttcaaacatt aattggtgat
     4081 agtgtgtatc tacttcagga taaatagaat ctcctgttgt cacgtccatt ttaaacggta
     4141 cacgtacttt ctctaaatta gccaccaatt taatttgaaa tccaggataa aaatcggctt
     4201 ctcttgtttc cttcattcct tgaatagaga aaacaatgcc ttcttttgtg ggttttctag
     4261 ttaaatcatt aaaaatgctc ttcaattttt cttcagttaa tttgctattt ctaaatgtcg
     4321 tatcgatatc aattgttgtc cttctatcga ttccatattt tgatcctaat aaaaagccgc
     4381 ctttcaaaat gaagtcattt ttgtactttg attcagaaat ttgttcaatt agacgatcca
     4441 tcataaaatg ccgttgcact aactgtgcat ttaatcctgt actctttgct aaatttcttg
     4501 tttttgctgc aaattgtgtc ggtgtcatct ttttcctcca tcaatataag ctttgcatgt
     4561 ggtatttcat ttctttttct acatgtagtt ttgtcatgta atctcttaat ttacttggat
     4621 ctctcaatgg gtcttccatg tactctttta atgcttctaa tttcatttca tattcaaatt
     4681 catatctcgg attccaaata tcacaaagtg tacgctcttt atcatacata gtaataggat
     4741 ttccttgaaa ggaagttccc tctacttttc ctagttcata aacttctttc ttcacgaaat
     4801 gaaattgtaa ctgctgctct tttgtaattg aacgatcaac gttatagcca tggggaaaag
     4861 ttaagtgaat gaatttcggc atattcgtag aaagctggta aatatctaaa gtcgtttcat
     4921 gagagaagat tcccctggaa aatcgttgct gaagccaaaa taaatcatct attccatgac
     4981 catcctctac ataataacca cgatcaactt tgatcaattt tccttgattc accaatcttt
     5041 gtacagtggt tgctgcgatt ccaaatttcc tggcttcctt catagataaa tttccatcgc
     5101 gaatagccaa caattctttc agtttatctt gcatttactt cccctccctt cgttacaaat
     5161 acactacatc aaatatatca tagtagttta tttgtaacaa gattaacgtc tatcgatcta
     5221 attcgatttc tttttgaatg cgctgctggt tctttaattg ttgcagtctt cttttatttg
     5281 cacgtaattt ctcttgttct aaacgaattt ttgctttttg attttcgatt tcttgttgtt
     5341 tgtgtgttgt atttcgcata ggataacccc ttcattattt tgtagtgcgg cgtgttcttt
     5401 tcttatagtt cgtttttttc ttttcagaaa attcaaagcc tgttgtaaag tcttctttta
     5461 aaacgatcgt tgcttcaaac gaacctttct gaccttttag tcctctgact gattggcttg
     5521 ttcggccgtt ttgaatgatc tctttcagtt gtgtggctgt gagtttcttt ttggcaatcg
     5581 ttgtccataa tttaaaatca caggcttcag acgtacatgc cgccacattt tgataaagca
     5641 agacttcttg ttcccgacat tttggacaaa cacctaaatg acgcgttgct tcctgttttt
     5701 cactggctgc tttcacttct tgtaacgtcg atccaaaatc agtggattgg atttgccctg
     5761 gtacgacttc caataagtga gagacaaatt tttggatatt ggctagaaaa gtggtttgtg
     5821 ttccttttcg ttcaccgatc tttaacaagt agctttccca tttggcggtc atttcggcac
     5881 tcgtaagcag cggctctttt tccacggctt ggcaaagcaa aatcccttta ttcgtcacca
     5941 cgagcttatt tttctctacc ttgatatatt ccttttgttt caaggtttca ataatattcg
     6001 cccgtgtcgc ttctgtaccg attccttcca cttctttcaa tatggattgc gcttcttcac
     6061 tatcaacggt tttcccagct gttttcatcg ccgtgatcaa cgtaccttcg gtataaggct
     6121 gtggtggctt ggtttctttt tcaaggactt gtagatctac cgtcaccatt tctccttttg
     6181 tcactttagg aagtagcggc tcttcttcct tctcttttcc gtcgttctct ttgccaaata
     6241 acttcttcca acctagatct atcggtactt ttccgattgc ctttaactga agctgctgga
     6301 cttgcgtcaa gatcgttgtt tcttcatagg tatatttttc cgcaaacatg gccaccgttg
     6361 tttttaacac ttgttcataa atggcctgct gcagtggact tagttgttca aaagccgctg
     6421 ctgtcataac cgtttttgta ggaatgatcg cgtaatgttc ctggactttc ttgttattca
     6481 cataccgact attcggcgta tgaatagggg ttgggattga ttcggccttt aaaaaatgct
     6541 tgtattcatc taaatgatcc aacaaatacg catactcccc ttccgtaata taaggggtgt
     6601 ctgttcgtgg atagcttaga tatttccctt catacaagcc ttgcatggct tccaaagtgt
     6661 ctttggcact agctttcatg agctgattca ttttagactg aaggctggat agagaaaaaa
     6721 gacgtggact tgccgcgctt tttttctcgg tttcaacttg gcgaatcatg cctggctgtt
     6781 ttcccaattg aacccccttt tcttttaaat aatcttcaaa tgcttcttgc gtcgcttggg
     6841 tttcattcgg atcaagcttc gcatcaaatg cgccattttg agcgattact tcggattttc
     6901 cttcaaaata aggttctttc ttgaaatttc taatcttttc ctgcaattga taaatcatgt
     6961 aaagcgttgg ggtttggact ctgcctaagg aaaagcttcc tggtatgcct ttttgctgca
     7021 agagtaaact atacagtggg ctaccgttca tcccaattaa ccaatcggcg atctgacgtg
     7081 cttgtgcttc ttcaaatttt ggataggtct cttttgcgtc tcgtaactgc tggaatccct
     7141 gataaatggc ttctttttct aaggagttga tccaaagtct tttaaaggtt tttccttgat
     7201 cggcgcctac ttgttgaatg atcgtccaag cgatcagttc cccttcacga tcgctatctg
     7261 tcgcaataat aatggtgtcg gccgattgaa gttgctgttt caccaccttg aattgctttt
     7321 tcttatcttt aggaacatga tagtgataat gtgtagggaa aataggcaaa tgttctaatg
     7381 accattgttt ccaattttca tcgtacatat cgggcgaatc taattcgact aaatgaccaa
     7441 aaccatacgt gatcacagtt tcaccaggaa acaatcgatc ttggatttca aaatacccgt
     7501 cttttcgagt ggctttagag aagctatcgg cataagcttt cgcttgtgac ggcttttctg
     7561 ctaaaataac ggttttcatc atgacgattt cactcccttt cttgttgtct gccttgttga
     7621 aacaacatgg cacgtttttg ctttctgatt tcttgttgct ggatctcatg cttcaattga
     7681 tcgttccagt ccttttttac ttcaggtata aattcatgtt catgattttt atagcctttg
     7741 tagtattgtt ctgctttata agagaaaaga atctgcgcat taatatcccc tttttcttga
     7801 taaaaacgat caaaatcgta cgtaccatcc acgcgttcac aaacttttaa ttcttttgcg
     7861 acttctctac aaatatcttt caaattaatg gcttctattg aatcgttaac agaaagctgc
     7921 tggccaaaaa acgcatttac tttgtgagcg gaaaccagtt ggttcgttaa ttgcagattg
     7981 ttttctgtct tgtgtatggc cataatagct tctggtgtga ccttttcttc ttttgcgact
     8041 tcttcataaa tgattttgta ttcttttggc acttgccaat cattagcgat tcctcgttca
     8101 caacgtactt tctgattcgt aaatggatcg actgcgccca ttaattgttc tttttcgtag
     8161 aaaatatgtc ctgcacggtc attatccaca caaatctcga tggattgagg aaaggcttgt
     8221 ttctttctca attcagatac tgcctcacca aaataatgac tgatcacatg gtgttttaac
     8281 ccttccatag agactaacca cgtatcgttt aactgatcac gattcagtgc agcatagcta
     8341 agtaaatcaa tactactctc aaaaaatttt aactgcttag ggtcaccgat ctttaggtta
     8401 aagccatgat tcgctgtcga atttttatct atatgcttat atgtgcctct ctcaccaaaa
     8461 cgttcataat caatgaacgt tccttgataa ctcgccccta ctgtctcttt tgtctgtgga
     8521 tctttccata aaaaggcgac cgcatgatct aaacgatcgt ctcgaactaa gaaattttgc
     8581 cgttcatatt ttggctttag ttcggcaatc aacccttctt tttctgcata atcaatcagc
     8641 cactctggta gttttcgttc ttccttcaga taattccttc ctttttctgt gagtggttga
     8701 taatcaacgg ggtgatcttt gtacttttct gtttcaaaat gaagattttc cactcgttct
     8761 gttttgtctt cattctttag aataaaatca gtggctgctt taaattgttg cttagagtct
     8821 tggatctctt tgtctatgaa aaaaagttta gctaattcaa taatgtcgcc actaatattt
     8881 tgactgttcc aataaaaacg ttgttttctt cgatcaaaaa caaatgaatc atggtcttct
     8941 aagcgatagt ctcgtccttt attgactaca gccattccat tgtttcgagc aaaatccact
     9001 atatcgatct gtttacatcg atcaataaga ctcatttttt ttcggtcatt ttcaaacctc
     9061 ccaaaagaaa aaagactatc cgtttttcag atagtctttt ttacttgttt tatacgcgtt
     9121 ctttttgtga caattgttgt tcataataga tttcattttt tcgtttggcc acttctgaca
     9181 ctgtttcaag tgaaaatcgg aatggatttc ctagcgcaaa ttcttctgaa ggtaacgccg
     9241 tttgaatctc ttttccataa ctttttcctt ttaaaatcac ttgattatgc tg
//
LOCUS       DAEGIQ010000099         9143 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000099 DAEGIQ010000000
VERSION     DAEGIQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.101"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            176..400
                     /gene="nrdH"
                     /locus_tag="KMT45_002244"
     CDS             176..400
                     /gene="nrdH"
                     /locus_tag="KMT45_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBE7005692.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            559..2718
                     /gene="nrdE"
                     /locus_tag="KMT45_002245"
     CDS             559..2718
                     /gene="nrdE"
                     /locus_tag="KMT45_002245"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBE7005693.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2768..3733
                     /gene="nrdF"
                     /locus_tag="KMT45_002246"
     CDS             2768..3733
                     /gene="nrdF"
                     /locus_tag="KMT45_002246"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBE7005694.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3822..4961)
                     /locus_tag="KMT45_002247"
     CDS             complement(3822..4961)
                     /locus_tag="KMT45_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE7005695.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5270..5983)
                     /locus_tag="KMT45_002248"
     CDS             complement(5270..5983)
                     /locus_tag="KMT45_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBE7005696.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6237..6938)
                     /gene="nagB"
                     /locus_tag="KMT45_002249"
     CDS             complement(6237..6938)
                     /gene="nagB"
                     /locus_tag="KMT45_002249"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBE7005697.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7172..8704
                     /locus_tag="KMT45_002250"
     CDS             7172..8704
                     /locus_tag="KMT45_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBE7005698.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8895..9074)
                     /locus_tag="KMT45_002251"
                     /pseudo
     CDS             complement(8895..9074)
                     /locus_tag="KMT45_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         3044 a         1783 c         1602 g         2714 t
ORIGIN      
        1 aaatttgcta ttttcccaca cgatacttgt tttcagctct tttttcaaaa acacaaaact
       61 tagtattcca cagtttccac aacacaatat gtagtgtttg ttgtttgact tttttactaa
      121 gagttttata ctatctatta tagataataa gaatgtgtta gaggaggcac tcaccatgaa
      181 cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag atggtcaaac ggtttttaac
      241 agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa cagcctgaag cagtagactg
      301 gttgaaagca caaggattcc aaagtgttcc tgtcattact tctgatgcag aaacagttat
      361 cggattccgt cctgatcagt tgcgtaaact tgccagctaa catgactaaa agaatcaaaa
      421 cgtgatacat ggatttgaag ggcttgagac agcaccagtt tttgtcgcaa acttccaaat
      481 ccttttttaa agcttaaaaa caatgcaatg atttgcaatc aagcatcatc aaatacgaaa
      541 tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag atgtcagtta tttcaagctg
      601 aataatgaaa tcaatcggcc agtcaatggt caaatccctt tgaataagga taaagaagct
      661 ttagttgcct tttttgagga aaatgtcaaa ccaaatacga tgacttttcc atctattatg
      721 gaaaaaatca attacttgat cgatcaagaa tatatagaag cagaattcat tcaattgtat
      781 tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt ttctttcaaa
      841 tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa cgacggttcc
      901 gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt tgcgaacggg
      961 gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata tcagccagct
     1021 acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc ttgtttcttg
     1081 actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc tttgcaattg
     1141 tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc cggcgctcct
     1201 atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt attcgaagat
     1261 agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt ttatttgaac
     1321 gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc agatgaaaag
     1381 attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta tgaattgaca
     1441 cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga atatggtgtc
     1501 ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa tccaaacatt
     1561 cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt acaacaagaa
     1621 tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc aatcgatggc
     1681 aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc ttctgtgatt
     1741 aacggcaaac aggaatatga agtattagga acagatatct catgtaactt aggctcgaca
     1801 aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac gatgactcgt
     1861 gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat ccaaaacgga
     1921 aataagctga atcatacgat tggattaggt gccatgggct tacacactta tcttgcaaaa
     1981 gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta cttcatgctg
     2041 ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa acaagttttc
     2101 cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata cgtaactgga
     2161 gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt tatcccaact
     2221 gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta tcatcaaaat
     2281 cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag tgcaagtatt
     2341 catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa aatttattac
     2401 ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta tgatatggac
     2461 atgcgtaaag taatcgatgt ttatgcaacg gcacaacaac acgtcgatca aggcatgagt
     2521 ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa gacaacaacg
     2581 aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa aggaatcaaa
     2641 tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag caatcaatgt
     2701 gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta attaaaggag
     2761 cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg aagatatcat
     2821 tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac gtatcccact
     2881 ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata cagtaggaca
     2941 tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca tggatcagtt
     3001 gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc aattcatgga
     3061 atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga aaaaagaaat
     3121 cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag ctgagcgtat
     3181 caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg tctttttaga
     3241 aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa acaacaaatt
     3301 ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac atggaaccta
     3361 catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac aagaaacgtt
     3421 aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac gttatacaga
     3481 agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac gctataatgc
     3541 caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg ccaatgatgt
     3601 caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact tcttctcaca
     3661 agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg attatttgat
     3721 tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa aagagtttag
     3781 cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact tgtttcttcc
     3841 agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa taaatagatt
     3901 ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca aatcagcatg
     3961 cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt caaaggatgg
     4021 atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa ttgttggatc
     4081 actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa accgacaatg
     4141 attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt gattccgcaa
     4201 atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa aaagctcatt
     4261 acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa tgtttcgttt
     4321 aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa gtgagacaac
     4381 gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg aacggtaaat
     4441 aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc agataaactg
     4501 actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa ttgttcttcc
     4561 aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt gcttgtttca
     4621 tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa ctgaggcact
     4681 acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa taaaaaaccg
     4741 aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa catataatag
     4801 ataaaaccag caacaagcaa tggtaaaaaa attgccgaaa gcattttcaa tattggacta
     4861 aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac tagctccact
     4921 gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct ccaaggtcct
     4981 ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc atttgcttaa
     5041 aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac aaaaacacgg
     5101 tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg gtgttcaaaa
     5161 gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat ttttatttgg
     5221 ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt tattgcgcaa
     5281 tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg atcacatcgg
     5341 cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact cggtagtcat
     5401 caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct tcaaaataat
     5461 cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac tttttagtcg
     5521 taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg ataaaactga
     5581 tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca attgcccata
     5641 aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc gttcccgcgc
     5701 ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct gaacgaatcg
     5761 ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg atctcaactt
     5821 ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc atacctgctg
     5881 gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata gcgatcccta
     5941 aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca ccctccagtc
     6001 caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct cttacgaaaa
     6061 aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg ttcaatcagc
     6121 tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc ctttgtcttc
     6181 tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat tgaaaattac
     6241 aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt ttgcaataca
     6301 cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc atcagctttg
     6361 ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc gattcccatt
     6421 gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt tgcattgatt
     6481 gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc aggttcattg
     6541 aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg atgtgcatcg
     6601 atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc atctggtaca
     6661 aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg acggtagctt
     6721 tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt catttcactg
     6781 aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct tcctgttgcc
     6841 aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa tgcttttttt
     6901 ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc tttatatttt
     6961 ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat agaccaaatt
     7021 gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca taagatggtt
     7081 caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg acgctttatg
     7141 tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa acaacattac
     7201 ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa atgctggcga
     7261 acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta aaatcatcac
     7321 tggtgagatc ttaccagatg acggacagat ccattggcaa aaaaatattc acaaaggtta
     7381 cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc taaaaactgc
     7441 gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa catatagcga
     7501 gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat tagaccaagg
     7561 agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg ggatcgatgt
     7621 cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa aggtgatttt
     7681 agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa ccaatcactt
     7741 agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg gtgcgtatat
     7801 cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg cagacatcga
     7861 gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac aaaaggaaca
     7921 aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg aaaagaccga
     7981 agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa gccgacaaaa
     8041 acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag ctgtctttga
     8101 ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt tagtcatcgg
     8161 atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg aaaatattgc
     8221 cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga tcggtgagat
     8281 tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt acttttcaca
     8341 agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca gtcaatttcc
     8401 aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag ttaatcaatt
     8461 agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga aacttgcaca
     8521 gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc atatcgatca
     8581 agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag taatcatcgt
     8641 ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga ttgaaccaaa
     8701 ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt gtcacaactc
     8761 cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca aaaaacggca
     8821 aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa cagcttttca
     8881 caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa aacaactgtt
     8941 tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc taaccagcca
     9001 tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat aaagtacata
     9061 aacataaata cagacccgtg gtgctagtta attttaatat cctaaagaat atctcaaagt
     9121 tccttcaaaa cgtcgttgtg ctc
//
LOCUS       DAEGIQ010000100         9056 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000100 DAEGIQ010000000
VERSION     DAEGIQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.102"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..812)
                     /locus_tag="KMT45_002252"
     CDS             complement(<1..812)
                     /locus_tag="KMT45_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBE7005699.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFR"
     gene            complement(785..1465)
                     /locus_tag="KMT45_002253"
     CDS             complement(785..1465)
                     /locus_tag="KMT45_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBE7005700.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1488..2426)
                     /locus_tag="KMT45_002254"
     CDS             complement(1488..2426)
                     /locus_tag="KMT45_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBE7005701.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2671..3534)
                     /locus_tag="KMT45_002255"
     CDS             complement(2671..3534)
                     /locus_tag="KMT45_002255"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBE7005702.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3676..6249)
                     /gene="mprF"
                     /locus_tag="KMT45_002256"
     CDS             complement(3676..6249)
                     /gene="mprF"
                     /locus_tag="KMT45_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBE7005703.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6390..7196
                     /gene="proC"
                     /locus_tag="KMT45_002257"
     CDS             6390..7196
                     /gene="proC"
                     /locus_tag="KMT45_002257"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBE7005704.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7275..8831)
                     /gene="rny"
                     /locus_tag="KMT45_002258"
     CDS             complement(7275..8831)
                     /gene="rny"
                     /locus_tag="KMT45_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBE7005705.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2670 a         2011 c         1563 g         2812 t
ORIGIN      
        1 cttctgaagg caaccaatat tcgtttgatg atatttacaa tcaggaacaa agcaataaag
       61 actaatgtga ttgttgctcc tggaggtgta tctagataaa acgagctagt tagtcctgac
      121 agcatcccta gaaatcctac acaaacactg atagcaatca cgacattgaa gcttttgcct
      181 aatcgcatac cgattgctgc gggaagaacc atgattgcgg atatcaaaag tgcaccggca
      241 atcgggatca tcacagcaat agccacacca gtaatcacat tgaacatcat cgacatcaaa
      301 tgaacgggta gcccatccac gtgggcggta tcttcatcaa aagtcagaac atacatcggc
      361 cgtttgaata aaaagaacaa tataccgatg gcaacgaata agattcctaa aaagatgact
      421 tgctcccatg taatggtcac gatcgaacca aacagatagg attggatact tgttgctgaa
      481 ttccctccgc tcaaattcat caatactaac gctagcgcca aaccaccagc cattaaaatg
      541 gcaatggaga tttctgaata ggttcggtag atcgttcgca gatactccaa tatcacagct
      601 gctaatacaa cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac
      661 gctacccctg ctaaagagac atgagaaagc gtatcagcca tcagcgattg tcttctgatc
      721 actaaaaaca cacctagcat cggggcgatt ccggcaatga agatagcggc aagaaacgct
      781 cttctcatga attcatatga aagcaacgcc attgagaatc ctcctttctc accagtcgga
      841 tctgtctgtc cgcatactgt ttgatatctt catgatcatg ggtgatcatt agaactgatt
      901 tcccatggac atgcgcacta tgtcggagaa gacgataaaa ttcatttctg gattcttcat
      961 ccatacctgt tgttggttca tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg
     1021 ctaaactgat tcgctgcttt tgtccgccag acaattcacc tattcgccga tttcgcatat
     1081 cccacatccc tacagcttct aaagcacgct gtacatgttg atgatctttt tcagagagac
     1141 gcttgaacca gcggcctctt ggatatcttc ctgaacggac taattcgatc accgtactag
     1201 gaaaacctgc attgaaagat gcaacttgtt gaggaatata accgatactt aatttttttc
     1261 cttcttgatt tttctcagca atctttatca ttccttttga aggtttcagt aatcctaacg
     1321 atgctttcac caaggttgac ttggctgctc cattttctcc agttaaaatg acaaattcac
     1381 ccgcatctac atgataagag acatcttcta gtacaggttc atcatcatag taaaaggtca
     1441 aatcagatat ttcgatataa cgcaccttac aacctccatt ctaaaactta attgatactt
     1501 tcttttaaag ctttcagatt tttttccatg acagaaatat aattttcgcc tgctttgatt
     1561 tgttcattag tcaagctttc taaaggattc aacacttcta gcttcactcc tgtttcattc
     1621 gacaatgtct cagctacctt cgaagaggca ttttcttcaa aataaatcac tttgatgttg
     1681 tttttcttca caaattcttt tagctcagct aagcgacttg gtgtgggttc ctgatctggt
     1741 gaaacgcccg aaatcgctac ttgttttaag tcgtattgat tcgctaaata agcaaaagca
     1801 gcatgctgag tgacaaatgt gcggtttgtt gcatctttta gctcatcgga gtatttttga
     1861 tccagtgctt caagcttttt gatataagca tcggcattct tcgtaaaagt ttccttttta
     1921 tctggatatt tttcgcctaa ttcttcagca atcgttctga cttcttttat agctaatacc
     1981 ggatcgagcc aaacatgtgg atcgtattca tgtgaatgac catgcccttc ttctgattct
     2041 tcatgttcgt ctgcttcttg cgattctaaa cggtcgattc ctttagctgc ttcgatcatc
     2101 aaggtctgtt tgctatccac tgaatccttt aacgaatcca caaaaaattc catgtctgaa
     2161 ctattataca cgaaaacatc tgcatcggaa atttctgctc tttcttttgc acttggttcg
     2221 aaatcatgcg gctccgttcc agcagggatc aaaagtttca cgtttccttc atcccctacg
     2281 atctcttttg taaaatcata catcggataa aaagtcgtaa cgatcgtcaa atcctctttt
     2341 ttgtctgctt ccttgttcgt atttccacat gctgcaaaca gtaaagcacc tatcattacc
     2401 gtagctgtca aaagatattt tttcatttat atgctccttt caatgaacaa atcggaattt
     2461 tacgatttat taacgtgaac taatgtatca aaataaaaaa acacagtcaa tagaaatcca
     2521 tcaatttctg atttaagcat gtatattctt gattaaatcg taaaatggat gagttgctgc
     2581 tcatatctag ccatttcact acaataaaaa actgaagaat accattttag gattcctcag
     2641 tttttcatca cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc
     2701 ctcacaaaaa ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa
     2761 ggcaaaaacg gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat
     2821 acgctctttg atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt
     2881 tcccattttt ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg
     2941 gatgtcttga tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac
     3001 actcattctt ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat
     3061 ttcttcccct ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt
     3121 accgacttcc aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg
     3181 gttgatccct cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac
     3241 tggaatattc ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag
     3301 caataaagca gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt
     3361 ttctacaatg attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac
     3421 gctcgacatc accatttcca actcatgata cccatcttgc cgtctatata atacatccag
     3481 tcctaaattt atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa
     3541 agtctaagtc gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta
     3601 gtggttgatt ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac
     3661 aagttctagt gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc
     3721 tttttctttt cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt
     3781 cctataagca aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc
     3841 agactggaag gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg
     3901 aaagaaagaa aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc
     3961 cgagtagccc attttctcgt atacaataaa cagattggct aacagatagt cagctaattc
     4021 ctcagattct ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg
     4081 ctgagcggtg atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga
     4141 taatacatat gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag
     4201 ccatttttgt gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca
     4261 agtaaaatgg tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga
     4321 caagtcttct gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc
     4381 caattcatga agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc
     4441 taaacaatct gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc
     4501 aactggatct cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg
     4561 atcttggtcg tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct
     4621 tgtagctgtg cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact
     4681 gccaagcttc gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga
     4741 gatgcccaaa ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg
     4801 atttcctgtt attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac
     4861 gataaacaaa aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa
     4921 ttttgttcga taaaattctt ttctggataa ccacacagct aacagaatca agacatatac
     4981 caatatcaac tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa
     5041 agcaatcgta ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag
     5101 taataaaaag ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa
     5161 taatacttga aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat
     5221 catgatcccc gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa
     5281 cagtcgcgga atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa
     5341 tagaagtccc gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac
     5401 tactgctaca tcttgccgaa cagacaattg actcattcct aagatcatca aaacgtcaaa
     5461 agtccccatt ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat
     5521 aggatagatc gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac
     5581 cgcaagggct cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg
     5641 ataaaactcg aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc
     5701 taaaaaacta ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat
     5761 aaaaaagaca tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag
     5821 tgcgactttt gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact
     5881 agcccgcaga cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt
     5941 tacccacgca gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa
     6001 gactctgatc gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag
     6061 aatcatgagc aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca
     6121 actcatgcca ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa
     6181 aaaaatcagt tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt
     6241 atttttcaca ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct
     6301 tatttatttt aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa
     6361 gatatcctaa tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata
     6421 tggcacaagc tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt
     6481 tcgtttccag tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg
     6541 catgcaaaag taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac
     6601 caaaaatcat tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca
     6661 tttctattgc ttcaggaaaa tcattaatag atttagaaac agcgcttgaa gatcctcagg
     6721 cctccatcgt acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga
     6781 tttgcaaaaa tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag
     6841 caatcggcag cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg
     6901 gcagctcacc cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga
     6961 atggtttgtc caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc
     7021 aaaaattcct ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg
     7081 gcggtacaac tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca
     7141 tcaaaggaat caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat
     7201 agcaatgttt tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc
     7261 ttttgtattt tttattattt agcataatca gttgcacgag tttcccgaat aacgattact
     7321 ttgatgtgtc caggataatc cagttcttct tcgattcgtt tacgaatatc acgtactaaa
     7381 cgaacagcgt ctaaatccga gatttcttct ggcttgacca ttacccgaac ttcgcgtcca
     7441 gcttgaactg cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt ttccagactt
     7501 cttaaacgac ggatgtagtt ttctaatgat tcacttcgcg caccaggtct tgctgctgag
     7561 agcgcatctg ctgcagctac aaggacagag ataacagaag tagcttccac atctccatga
     7621 tgagaagcga tcgcgttgat tactactggg ttttctttgt acttggcagc taattccgca
     7681 ccgatctcaa cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat atcatggagc
     7741 aacccggcac gttttgctaa ttggatatct tcgccaagct ctgctgctaa gataccagct
     7801 aattttgcaa cctcgaccga atgcttcaag acgttttgtc cataactagt tctgaatcgc
     7861 agacgtccga tgatcttgat tagatctgga tgaagtgtat gagcaccgac ttcaaacgct
     7921 gcctcttccc cgtattcgcg aatacgttca tccatttctt tacgagattt ctcgaccatt
     7981 tcttcgatac gcgctggatg gattcgtcca tcttgtatca gtttttccaa ggtcatccgt
     8041 gcgatttctc ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg
     8101 atgattaaat cgataccagt cagcgtctca agcgttcgaa tattacgacc ttctcgtcca
     8161 atgatacgac ctttcatttc atcattcgga agggtaacga ctgaaacagt cgtttctgaa
     8221 acttgatctg ctgcgcagcg ttgaatcgct aatgataata agtttttcgc tttgcgatct
     8281 gcttcttctt tggcacgctg ttcagattct tttaccatta gagttaactc atgattcagc
     8341 tcttcttcag tcgatttcat gatgacatct cgtgcgtcct cttttgataa accagcaatt
     8401 ttttctagtt ctttttgttg atcttcaatc attttttcaa cgtctttttc tcgctcttca
     8461 attaattgct gttttgaact aagtgcttct tctttacttt caagtgagtt ctcgcgtttt
     8521 tctaacgagt catctttacg attcagcgtt tgttcgcgtt gaatcaaacg attctcttga
     8581 gattttaatt ctaacttact ttctctcagc tcactttcaa cttcagaacg gtatttctga
     8641 ttttcttcct tagcttctaa caatgcttct ttttttagag tttctgcttc tttttttgca
     8701 ctatcgatga ttccagcagc tgatgattgt gcaccagcta tttctttctc gtgacgtgac
     8761 tttgcgacca taaaccctaa accaagaccg acaattaaac cgatgatagc gaggagaatg
     8821 ttgaatacca taaaatccac ctccatacta tctttttcat ttttatcatc tatacagctt
     8881 ctatgataaa ttattctaaa ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac
     8941 atgcacaact ttattctaat gtttgtaccc cgtagtgtca actttaaata aacaaagaaa
     9001 cggctttcta cttattcatt aaacgatggt ttttaaaata aatgacacta gtttca
//
LOCUS       DAEGIQ010000101         8736 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000101 DAEGIQ010000000
VERSION     DAEGIQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.103"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(230..2611)
                     /locus_tag="KMT45_002259"
     CDS             complement(230..2611)
                     /locus_tag="KMT45_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBE7005706.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2601..3248)
                     /gene="recU"
                     /locus_tag="KMT45_002260"
     CDS             complement(2601..3248)
                     /gene="recU"
                     /locus_tag="KMT45_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBE7005707.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3312..3851
                     /locus_tag="KMT45_002261"
     CDS             3312..3851
                     /locus_tag="KMT45_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBE7005708.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3941..4360
                     /gene="gpsB"
                     /locus_tag="KMT45_002262"
     CDS             3941..4360
                     /gene="gpsB"
                     /locus_tag="KMT45_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBE7005709.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4382..4749
                     /gene="rnpB"
                     /locus_tag="KMT45_002263"
     ncRNA           4382..4749
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KMT45_002263"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4958..6124
                     /locus_tag="KMT45_002264"
     CDS             4958..6124
                     /locus_tag="KMT45_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBE7005710.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6170..7666
                     /locus_tag="KMT45_002265"
     CDS             6170..7666
                     /locus_tag="KMT45_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBE7005711.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7747..8430
                     /locus_tag="KMT45_002266"
     CDS             7747..8430
                     /locus_tag="KMT45_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBE7005712.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2751 a         1581 c         1852 g         2552 t
ORIGIN      
        1 gtgtatttag ccgatttaat tataaacaag taaatggata aagtatatgt aatgataaaa
       61 gaaaaatgga ttttctaatt tttaagctaa atgttagatc acacatgtat atgctggata
      121 gtcttataaa aaatcatgta atacagcttg gtacatgatt tttatttgta gctgtttttt
      181 acatagacac aaaaaagaaa ccacgcttgt agacgtggtt tcttttttac tatctgtttc
      241 cagaagctga ggtgctgctg gatggtgtat ggtcatttgc agcatccgct ccgctagttg
      301 ctggctcact ggaagaagca ggaggagcag atgaagacgg tgtcgagctt tcttcggatg
      361 aagaagaagg aaccgttgtt gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt
      421 ttgaggactc agcagttgat tcgctttgac tagtagagct agagctttga gtcgttgtct
      481 ctgttgttga gcttccagga gtctggacat agctggaaga tggaatagtt gttgatggtg
      541 taattgcatt cgatctagct gtatattgat ctttgtagta cagttcaccg cccacacgaa
      601 ttaatccgct tggcatctcc cagtctgtat ttgtcacgtt agctgaaaca tactgcataa
      661 gctcgcgata aacatcagag gctacgtgtg acgattctga agtgacaggt gtcatttttt
      721 tattgtaacc tgtccacacg gaaatcgaat aatttggcgt atagcccgca aataggatat
      781 ctggatacac cccagaagaa ataccaagtt ttgcatactc gtcatctgtg taattggatg
      841 ttccggtctt accggcttga tataacccag cgatttgcgc attcgtacca gttccttcag
      901 tgatcgtatc ttttaaaatg tctgtgatca tataagctgt ttctggagac atggctgttt
      961 ttccatctgg ctcataagtt tcttctgttc cgtcttggaa gacgatttta ttaacatatt
     1021 gaggtttgta ataagtccct ccattggcga aagccgcata ggctgctgcc atttttaagg
     1081 aagaagctcc gtattttgtc ccatcttgtt cttccgtatt actagagatc gcattcgatt
     1141 gatggatcgt actgtattcg atcccaagat tttttaagaa agaagccact ttatctgaac
     1201 cgacttcatt gaataatttg actgctggaa cattacggga taagtaaagt gcttggcgta
     1261 aagtaatggt gcccatatat tgattatccc agtttcctac aggagtcgaa gtcccttcat
     1321 agttatacgg tgcgtcagta atagtttttc cagtagaata tttcaaatat tcaaaagctg
     1381 gtccgtaatc tgtgacaggt ttcatcgtag aaccaaaatc acgggaagta ttcactgcta
     1441 agttgttgcc taaagttacg tcttctgcga tgtgacgtcc gccaatctgg gcttttactt
     1501 tcccggtatt ggtgtcgata agggtggagg caacttgcat ctcgtcgtct gggtaagaga
     1561 catactgatc cgtgttgacg atatcatata gtttcttttg ggcgtccaga tccaaattcg
     1621 tataaatctc taaaccgtct gtgtagacat tcttatctgt tttttcttgt acttcattga
     1681 tgacttcttt gacataatta tcgacgatct tagtattgtc atcggattga gttagttctt
     1741 gcagaccatc cgttaccggt acattaacag cttgatcata ttctgtttga gagatttttt
     1801 cgttttgaag catcgtatac aaaacagtat ctcgtctttt cttcgcctga tctggataaa
     1861 cataaggatc ataggcggaa ggggcttgag gcatcccagc aagcaatgct gtttgcggta
     1921 aagaaagttc tgataatttc ttaccaaagt acatctcaga agctgtctcc atcccgtaca
     1981 agccgtttga catatacact ttatttacgt agtaggtcaa gatctcttgt ttcgattttt
     2041 tttgttccaa tcttacagcc atccacgctt cttgggcctt gcgtttcaga gtctgatcct
     2101 cggcactggt ggagaagaag gaaagtttga tcaattgctg tgtcaaagta cttccgcctt
     2161 gcagtccgcc agaggtaaag ttagagaggg cagaaccgat gatccgaata gggtcaaccc
     2221 cgatgtgttt ataaaaacgg cgatcttcta ccgacacgat cgcatcttct aatgttttcg
     2281 gtaattcatt cgcagaaatt ttttcacgct tttctgcgcc taaatcttcg aatagttcgc
     2341 catcttgtgt atatagtttg gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg
     2401 gcgcatcttt tgcgtaatac cagaaaagtc ctacacctgc taaaaagagg atacagaaaa
     2461 atgagagtat tcctaaaagg atcttgataa ataatccttt tttatgtgtt ccagaggact
     2521 ttccagaacc tttgccagaa ttcttcttca cactcttttt aggagtaggc tgtttctgtc
     2581 gtctagagct tctagtttgt tcatttgcca ttcttgcttt ctccttcaat agataatgtt
     2641 gataaataga agtcaaccgc gtccaaataa ggaatccgcg gagaaatacc agttttgatc
     2701 tctattccta gttcttcgat caaagataaa ggtagtgatt tgcgtccttt ttccttttgg
     2761 gcgtcccagt aagcaatcaa atgaacacta ttcaaataaa aacagcgctt caaagacgaa
     2821 aaccataaca agacaaagca gaccccattt tgttcgaggc atgctttcat atgttcgatt
     2881 tgatgggcat ggaagttttt aaagggaaaa gatgttttgt tcttggtttc ttttgcttca
     2941 aagtccaagt aaaagcctcg atagacgcca ttatagtctg tagttgaggc ttgggtaaag
     3001 taggcttcct tgatcacagc agcacttctc ttaggatagt ctactttcac gatttggata
     3061 ggggtaggct ttttatggat aaccgccaac ccgcgggaaa gataatattt attactttca
     3121 ttgattgctt cttcaaagcg catcccccga ttgccgaatt cagtgatctg atttcttttt
     3181 tcttgaattt tttcgacggg agaggcattc ttataataag gttggccatt tggatagcgc
     3241 aataccatct ttaatcacac tcctaggcca cattatagca aaaaagcgga tagaaagaaa
     3301 aggaatctga gatggataag tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac
     3361 tcggtgtttt taaagaaaat gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag
     3421 aactccatca gttagcagaa gaagggctgg aatgggtact tgtcagtggg aatttgggcg
     3481 tcgaactatg ggctgtgcaa atagtagcgg aattgaaaga agaatatccg gaactatctc
     3541 ttggtatttt gtatcctttt tctgattttg gtaataactg gaatgaaggg aaccaagaaa
     3601 aaaaacaatt agcagaacaa ttagcagatt atgtcgaagc tgttagccat caaccctacc
     3661 aatcacctag tcagctaaag aatcacactc gttttatact tgagcataca gtgggctgtc
     3721 tgctgatcta tgatgaagaa ttccctggaa aaacgaagta tttttttgaa gatgcacaga
     3781 attaccaagc agatcatccc tacgaaattc ggctgatcag tatggacgat cttcaaaatt
     3841 ttagtgaata atcgatattg tgtttgattt atcaactttt ttcctataca atgaaaaagg
     3901 tagaaatgtt attacgtaaa tgaaaactag aggtgtaaat atggcgaatt tggtttatag
     3961 tcctaaagac attttacaac aagaattcaa aacaaaaatg cgcggctatg atcccgttga
     4021 agttgatgaa ttcttagaca atatcatcaa agattatgaa acttacagca aagaactcct
     4081 tgctttacaa gaagaaaacg atcgattgag tgcaaaagta gctcaattat ctaaaactca
     4141 aggagcagct caaacacgcg tgcaacaaac agaggcacca aagagcgcag cagtgaccaa
     4201 ttttgatatc ttaaaacgct tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga
     4261 tcaacaagca agtgcagtca aaccagcaca acctaatcca aataactaca caaatgcaga
     4321 cacaagctta gatgataatg aaaaaacacg tcaattttaa tttttgatta atgaatttgc
     4381 ggttttcggg taatcgcggt ctgacttgat caggctgagg aaagtccatg ctcgcacagg
     4441 ctgtgatgcc tgtagtgttc gtgcttagcg aaaccataag ctaaggtact ctttggagta
     4501 acggcaggaa aacaagctaa ggcttcggct atgcttaagt atccttgaaa gtgccacagt
     4561 gacgaagcgg ctggggaaac ccatccggtg gaacgcggta aacccctcga gcgagcaacc
     4621 caaacaatgg taggggcgct ttttgtctgg aagaagaaca ggacaaaaag gcagtataac
     4681 tgcagataga tggttaccgc tttgtttttt ctccctggaa aagcaaagta cagaacatgg
     4741 cttatagaaa accgcaaaca tcagggaatt aggtcgtgac aaaagtaatg cgttataaga
     4801 aacaagaaaa acatttgaaa gctatttttc aatacttcag atgaatcgtt cttattacaa
     4861 aacaaacttt tgtatcaccg gttattcgga atttgagtaa aagtgtgggg taggactgat
     4921 gtcccacctc ctttttttat aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa
     4981 cctcgtcgca actgcggcaa gcgggctaga agcactggtt ggtaaagaat taagagatct
     5041 tggaatcgaa tgccaagttg agaacggacg tgcacgtttt gaaggaacaa tggaaacgat
     5101 cgctacagct aatttatggt tacgtacagc agatcgagta aaaatcgtgg ttggggaatt
     5161 cgatgcgtat acgtttgatg aattgtttga aaacgtaaaa gcattgcctt gggaagattt
     5221 tcttccacta gatgcagctt ttcctgtagc tggaaaatcg atcaagtcga aactatatag
     5281 cacacctgat tgccaagcga tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta
     5341 tcaccgtcca gcttctgtac cactgacgga aactggcgca cattttcagt tggaagtagc
     5401 tttgctgaaa gaccatgtga tggtaactct ggatacgaca ggaccaagtt tattcaaacg
     5461 tggttatcgc ttagaaaaag gtggagcacc attgaaagaa aacatggcag cagcacttat
     5521 tatgttgaca aattggcgaa aagaccgtcc attttatgat cctgtatgtg gttcaggtac
     5581 gctttgtatt gaagcagcct taataggcca taatatcgca ccaggattca atcgtgagtt
     5641 tgcttgcgaa tcttgggatt ggttcagtca agaaatcatg gaaaatgttc gatcagcagc
     5701 cgaagaaaaa gcagattatg atattgaatt agatattact ggttcagata tcaatggacg
     5761 gatgattgaa attgccaaag ccaatgcaga agaaatcgga ttaggctctt cgattacctt
     5821 taagcaacaa gcagtcaaag acttcaaaac ggataaagaa tacggggtca tcgtggctaa
     5881 tccgccttac ggagaacgtc taggagaaga agaaactgtt cggaaattat ataaagaaat
     5941 gggcgatgta ttccgtccat taaaaacatg gagtaaatat atcttgacta gcgacttagc
     6001 gtttgaggaa ttttacggac aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt
     6061 gcgtacggat ctattccagt attggggaac tcgtccgcct agaaaaaaag agaaagacga
     6121 ataatcttac taattgattc attcaattgg atgtttggag gaatagggaa tgaaagaaaa
     6181 agagttttta caagaagtaa aagaaatcaa tcttctacaa caagctttag gaatcctcga
     6241 ttgggataca caaacaggta tgcctgaaaa agctagtgca taccgaagtg aagtagacag
     6301 ctatctttac agcatttatt tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg
     6361 ctatttttct gaacatccgg aagaattgtc ggaagttgga actgccgttt ttgccaaagt
     6421 aaaagaagag tatgatttgg aacatcgcgt accggaagat cttatgagaa aatatacagc
     6481 tgccgtttct tcagcacacg gacaatggca aaaagctaga gaaacacaac aattttctga
     6541 ttttcaacac gcattgaatc aaaatattga attaacaaaa cagttgatac cttactggag
     6601 aaaagatgaa aagacgaatt atgatgtttt gctgaatcaa tacgaacccg ggatgactgt
     6661 cgaaatcctt gataaggtat ttgatcagct taaagaagga attctttcga tccgccgtac
     6721 gctccaagaa aaagggactg aacctgaaac agacttttta aatcggagaa tgacgaaaaa
     6781 acaacagaag agatttgtag tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg
     6841 tcgtttagat gacactgtcc acccatttat gactggaatt aatccgaatg atgcacgaat
     6901 cacgacacga tggaacgaaa cagatttcaa aatggctgtt tttggagtga tccacgaggc
     6961 tggtcatggt atgtatgaac aagacatcga tgataaatat gcgtatacac cggtttatga
     7021 aggtacatca atgggtatcc atgaatcaca atcgttgttt aacgagatca ttattggaag
     7081 tagtcgcagc ttctggaaaa agcaatatcc tttctttcaa gaatgtgcag aaggaacatt
     7141 cgatgatatt ccttttgaca aattttatcg ttcattgaac catacaaaat caagtttggt
     7201 tcgtatcgag gcagacagtt tgacttaccc tctccatatc atcattcgtt atgaaatcga
     7261 aaaaatgatt ttcaatgaag gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa
     7321 atatgaagag tatctgggaa tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt
     7381 tcattggtca ggtgcaagct ttggttattt tccctcttat gcattagggt acatgtatgc
     7441 ggcacaatta cttcattctt tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga
     7501 cgattattct ccaatcaaaa actggatgtc agagcatatc catcaatatg gtgcatctag
     7561 aaaaccaaat caattaatca tggatgcaac acatgaaccg cttaatccgc aatacctgat
     7621 cgactatatg aaagacctgt attttaatgt atatcaggta aaataaaata caatttacaa
     7681 agatagctga aattcatcta atctatagat gttttcagct atttacttaa gaaagaagtg
     7741 gaacgaatgg ataaattaca agtgatcgct gattatagta tggaccaatt agcgcatgat
     7801 caaacaggac atgggagcga tcataccaaa agagttgtca agttagctga aaaaattttg
     7861 gagacggaac cgcaggcaga ccgctttgtg acgcttgcag cagcttacct acatgatacg
     7921 attgatgata aagtcgttaa aaatgagaac gaggcaaagc aacagcttcg tgtattcctt
     7981 cgctctttgc caatagtaga ggaacagatc tcgatgattt tttccattat cgaaaatatg
     8041 tccttttcta aaaatctctc tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa
     8101 gacgctgatc gtatcgaagc attaggagca atcggcattt tgcgtactgc ttatttcggc
     8161 ggtggacatg ggcacccgat ttttgatcct gaactgtatc cgcaaacatt caaggacaaa
     8221 gaaaattacc gaaaaggcac gacagtgatc aatcattttt atgaaaaact gttcttgctg
     8281 tctgataaaa tgaatactga ttacggatat gaagaagcca aacgaagaga aaccttcatg
     8341 cgtgaattct tagaagaatt ttttctggaa tggtctattg acgatgaaaa ttttactcca
     8401 gacaattgga tcaaaaataa caaaaaatga tggaggttgt gacagaagtg gtcagctcca
     8461 agatatttcg gatcattttc cgtaaaagtt acttctggaa cactgtttac caagaggtcg
     8521 tgagcctgcc gttcagctcc gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt
     8581 tgatgaatat tgtaacttgt gattcggaat tggcaggaga acaccgttta ttcggaagaa
     8641 taggactgtg acaagacttt tgtcacagtc cttttttcga attcaacaag taaaactttt
     8701 aagaaatatt cagagagtaa aattaaaaat tgctga
//
LOCUS       DAEGIQ010000102         8673 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000102 DAEGIQ010000000
VERSION     DAEGIQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.104"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            62..349
                     /locus_tag="KMT45_002267"
     CDS             62..349
                     /locus_tag="KMT45_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005713.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            765..2303
                     /locus_tag="KMT45_002268"
     CDS             765..2303
                     /locus_tag="KMT45_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBE7005714.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2300..3265
                     /locus_tag="KMT45_002269"
     CDS             2300..3265
                     /locus_tag="KMT45_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005715.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3252..4574
                     /locus_tag="KMT45_002270"
     CDS             3252..4574
                     /locus_tag="KMT45_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBE7005716.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYFVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4637..4741
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4815..5558
                     /locus_tag="KMT45_002271"
     CDS             4815..5558
                     /locus_tag="KMT45_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7005717.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5573..6565
                     /locus_tag="KMT45_002272"
     CDS             5573..6565
                     /locus_tag="KMT45_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE7005718.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDLELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6577..7314
                     /locus_tag="KMT45_002273"
     CDS             6577..7314
                     /locus_tag="KMT45_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005719.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7304..8026
                     /locus_tag="KMT45_002274"
     CDS             7304..8026
                     /locus_tag="KMT45_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBE7005720.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8110..>8673)
                     /locus_tag="KMT45_002275"
     CDS             complement(8110..>8673)
                     /locus_tag="KMT45_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBE7005721.1"
                     /translation="MGTTAHADEAYTVQSGDTLSTISQKYVGDNSLINAIAESNSISD
                     INLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAPVAETQP
                     APAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDYSAANQE
                     RVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2799 a         1485 c         1871 g         2518 t
ORIGIN      
        1 gaaaatgaaa aaaaattatt tttggctgtt tctttttatt tatcaaataa ggagtggaaa
       61 aatgcgagca gcagacttat tgacaattct tacaaaatca cccaaaaaaa cagagtatca
      121 aggagttttt ttggctcaag aacaaaaact ggtgcctgtg acaatggtcc agataaataa
      181 agcaaatcaa tttatctttt tatttgaaca cggcaaagca ccacttgcta tgaaagacat
      241 ctttcttttt ttgatgaaga accgtgagaa agctgtttat tatcagtcaa aagaagaact
      301 cattccgctt tatggagtga aagagataga aaataaagtg gtgatataag taaaaataag
      361 aaaacaagac gtttatgatg tcaaaacaac cgttttttac atttttggca aaaaagtgtc
      421 atgatacagt ataatattcg ttgaacaggc caataaaggt gttctccccc acgagatttc
      481 tactttattg gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa aagatatagc
      541 tgattccaag tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac
      601 caaaaagttg atccgacttt gttgcttgga ttcttttttt gtcagcatgt ttagaggtat
      661 ttttttcttt tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag
      721 gaaagaaatg tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac
      781 aaacattaag tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt
      841 ctctcatttc tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca
      901 atcataaaaa aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt
      961 cacttgcctt attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga
     1021 ttgcacttgt tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg
     1081 aaattgaccc gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct
     1141 tgctgaaagt agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct
     1201 tagttttaat catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga
     1261 catttatttt gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca
     1321 gttctgcctt gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac
     1381 ctcgatattc catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat
     1441 atcagtctgc cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac
     1501 ctgaaatcct gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg
     1561 aattaaaacc gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa
     1621 tcgatatcta tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag
     1681 caatgaaaag tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga
     1741 ataagcaatt cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg
     1801 cagtcaagag atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta
     1861 tctctgatca gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata
     1921 aaggaatcca agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt
     1981 tgcgttacca agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata
     2041 gccagctcaa ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt
     2101 atcaaggcgc accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag
     2161 cactgaataa attaagcgga aaaataacag aggatcaaat gattgatatg aattaccaag
     2221 tcaatgtcga aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat
     2281 tagtaaagga ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta
     2341 tgatggaaag tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt
     2401 tttggttggt ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc
     2461 taaaccatca gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca
     2521 gaaaatgcgg tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac
     2581 agtcaagcaa aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc
     2641 agaccgtgta gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg
     2701 tatgcctaga gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc
     2761 tgcaagccca gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag
     2821 aaactcatta caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt
     2881 cgttacgcac aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga
     2941 tggagaaatt atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta
     3001 cgttcggcat ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt
     3061 cgtcaatgga tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga
     3121 tacgcctatg aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga
     3181 aaagcaaaga agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga
     3241 ggaggaagga aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa
     3301 gtgctgctta tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat
     3361 ggggcgggac ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag
     3421 aggcacgtaa tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca
     3481 actgggaaga acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa
     3541 aaaaacaatt agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg
     3601 attcatctat tgaagtcaac gaagaagtct tccatgcgga ttactttgtt ttggcaaccg
     3661 ggcaaagacc tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact
     3721 ttttatcatt gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt
     3781 ttgagctagc tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc
     3841 aacggccatt aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag
     3901 catctggcat tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc
     3961 gctatcgttt agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg
     4021 ggagacagcc gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac
     4081 acggaattgc agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag
     4141 atattgtttc aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg
     4201 tagctaagcg gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga
     4261 tcgtctacgc cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt
     4321 cagatcaagt ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac
     4381 cagttgctaa ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga
     4441 ttagcgagca agcagacgaa ttgatcgatg atttgacact agtcatcaat caaaaattga
     4501 ctaaaaaaga gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct
     4561 atttattgaa ataatcagca tgataactaa agcgcttgtt gctttttcgt tcttgtgcta
     4621 cactttaaaa aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt
     4681 gaacttagac ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc
     4741 ttgaagcggg ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga
     4801 aaggaggtaa caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa
     4861 ttatttggca gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg
     4921 ctgctgtatt ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga
     4981 cattaaatga agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct
     5041 tggcaatgga ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac
     5101 aaatgttgcc aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc
     5161 ttccaaaagt gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg
     5221 atacattgag gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata
     5281 cagggaagat ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt
     5341 taaaagtggc tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc
     5401 aagcaggctt agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt
     5461 ttgctgcgat catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat
     5521 tgaaaaaagg gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa
     5581 attatggctt ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac
     5641 aagtagcaaa caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc
     5701 gaatactaat catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg
     5761 tttaaatgtc caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc
     5821 ggacaaagca caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg
     5881 acagaatata ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc
     5941 caccctatca gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg
     6001 ttggcaaagt ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga
     6061 cttttcaaaa ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc
     6121 ctcagatatc caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat
     6181 tgaactaaac tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt
     6241 aattattacg aatgatcaac tgatcaaaag tgatctagaa ttggtacagt cttttatgga
     6301 tgccacgaaa aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt
     6361 gcaaaaatat gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc
     6421 aaaaaattat acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa
     6481 ttatacagct tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact
     6541 attcactaat cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg
     6601 aatgtagtca ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat
     6661 acctttacat cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta
     6721 acaggagtcc agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt
     6781 ctccatacag catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc
     6841 tatcaaaacg ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta
     6901 agggattatc tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta
     6961 tctggcggga tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac
     7021 ttgcttcttg atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac
     7081 tggatgttga agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc
     7141 gaagaagcaa tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg
     7201 attgctgaga ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca
     7261 gagttgaagc aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca
     7321 ttgtcattta gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac
     7381 gatcatcaat tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa
     7441 gactcaagcc ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca
     7501 agttgagcct tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg
     7561 gacgaatgta ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc
     7621 cataaatgag aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta
     7681 tatccaagct taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca
     7741 agactatatt gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc
     7801 agtctggcaa ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact
     7861 acatgcagca gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct
     7921 tgaagaacag ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa
     7981 aaatacttat caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac
     8041 acaactagtg tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat
     8101 aaatgaatct tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc
     8161 ataacgtgaa gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc
     8221 atttaagtaa gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta
     8281 agaaccgctt gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt
     8341 agcagcagtt tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt
     8401 ttccgtttga accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac
     8461 ggctgcttga ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg
     8521 tcctgagtaa atcaggttaa tatcagaaat tgaatttgat tctgcaatgg cattgatcaa
     8581 ggagttatca ccaacgtatt tttgagaaat cgttgataga gtatcacctg attgaacggt
     8641 ataagcttcg tccgcatgag cagtcgttcc cat
//
LOCUS       DAEGIQ010000103         8453 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000103 DAEGIQ010000000
VERSION     DAEGIQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.105"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1329)
                     /locus_tag="KMT45_002276"
     CDS             complement(<1..1329)
                     /locus_tag="KMT45_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBE7005722.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIKPVVLVNFNILKNISKFL"
     gene            complement(1470..2072)
                     /locus_tag="KMT45_002277"
     CDS             complement(1470..2072)
                     /locus_tag="KMT45_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBE7005723.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            2317..2523
                     /locus_tag="KMT45_002278"
     CDS             2317..2523
                     /locus_tag="KMT45_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005724.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2835..3554
                     /locus_tag="KMT45_002279"
     CDS             2835..3554
                     /locus_tag="KMT45_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBE7005725.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(3635..4318)
                     /locus_tag="KMT45_002280"
     CDS             complement(3635..4318)
                     /locus_tag="KMT45_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBE7005726.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            4536..6722
                     /locus_tag="KMT45_002281"
     CDS             4536..6722
                     /locus_tag="KMT45_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBE7005727.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7068..7478
                     /gene="spx"
                     /locus_tag="KMT45_002282"
     CDS             7068..7478
                     /gene="spx"
                     /locus_tag="KMT45_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBE7005728.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            7492..7848
                     /locus_tag="KMT45_002283"
     CDS             7492..7848
                     /locus_tag="KMT45_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBE7005729.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3107 a         1382 c         1582 g         2382 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gttttatttg
       61 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
      121 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
      181 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
      241 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
      301 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
      361 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
      421 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
      481 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
      541 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
      601 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
      661 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
      721 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
      781 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
      841 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
      901 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
      961 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
     1021 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
     1081 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
     1141 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
     1201 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
     1261 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
     1321 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
     1381 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
     1441 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
     1501 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
     1561 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
     1621 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
     1681 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
     1741 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
     1801 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
     1861 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
     1921 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
     1981 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
     2041 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
     2101 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
     2161 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
     2221 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
     2281 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
     2341 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
     2401 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
     2461 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
     2521 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
     2581 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
     2641 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
     2701 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
     2761 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
     2821 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
     2881 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
     2941 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
     3001 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
     3061 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
     3121 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
     3181 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
     3241 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
     3301 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
     3361 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
     3421 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
     3481 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
     3541 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
     3601 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
     3661 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
     3721 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
     3781 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
     3841 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
     3901 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
     3961 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
     4021 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
     4081 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
     4141 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
     4201 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
     4261 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
     4321 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
     4381 aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt
     4441 ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg aaataaacaa
     4501 attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa attctccgat
     4561 tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa gtctttcggc
     4621 tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc ggacttattg
     4681 aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa atgatggaga
     4741 aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat gatatgaaga
     4801 aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc tgtgcaaaag
     4861 ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg atcactttta
     4921 caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag agaaaaaccg
     4981 ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa atttgtcgaa
     5041 aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat actgcacaaa
     5101 aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata tttgatcaat
     5161 atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac ctttcagtag
     5221 aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat gcacgacagc
     5281 cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa agaagccagc
     5341 aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag aatgaggaga
     5401 tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt caagatacta
     5461 atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc aagttgattg
     5521 ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca gcatacatca
     5581 agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat tatcgttctc
     5641 gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat gatcggatac
     5701 caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat ttgttttctg
     5761 atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt caaaaaaatg
     5821 ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag cagcttgtcc
     5881 aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca tatgaaggta
     5941 tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg caaacagcac
     6001 tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg atggcagact
     6061 gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa aatatagccg
     6121 ttgcagatta tattttgtcg ataagcccca atttaaaatc gaaatctgca gaaatccgag
     6181 agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg cttgttcaaa
     6241 gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa gaatggatac
     6301 gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg caagaaaccc
     6361 aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga aaacttgatg
     6421 cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat gtctttttga
     6481 ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa gtaatcgact
     6541 ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga agaacgaaga ctattttatg
     6601 ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg aaaaattcaa
     6661 gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa aaaggaaagt
     6721 gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg aatgtacgaa
     6781 aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta tttctggaac
     6841 acagtttatt cggaaaaggg aggtgtgaca agaattttgt cacacctccc ttatttattt
     6901 tgaaaatgag aatactatta tcaaaataga gaatatatag aaaaattctc attattcaaa
     6961 cgtaaataat cagttatttt gatttttttt atattttatt agctaataat tggtatttta
     7021 aaagaaatta ctgtagaatt ggattataat taaaaaaggg tgagaaaatg ataaaagtat
     7081 atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac
     7141 ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga
     7201 tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg
     7261 aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag
     7321 aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat
     7381 ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc
     7441 tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg
     7501 aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac
     7561 catcaattgc cttatcaatt tgttgactta gaagaacgcg atctaagtac agaagaagca
     7621 gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag
     7681 gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta
     7741 tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt
     7801 attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt
     7861 ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt
     7921 gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca atttatgtgg
     7981 tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg tgtaaattta
     8041 catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta ttttaaaaaa
     8101 catataaaat agttgttgac atgttagaaa tgacatgata agataactga gttgttaaaa
     8161 aagaaccact tgtgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
     8221 aacaaatgat aagttatgat aaatgagttg ctgtaagatt tcgaaaaacg aaatcaaaaa
     8281 aacttttaaa aaagagttga caattacttg tctctctgat atgatataaa agttgctacg
     8341 gcagccaaaa acgaatcggg gaacactcct gatgaagtag acctttgaaa actgaacaaa
     8401 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa aca
//
LOCUS       DAEGIQ010000104         8420 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000104 DAEGIQ010000000
VERSION     DAEGIQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.106"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(289..966)
                     /gene="nth"
                     /locus_tag="KMT45_002284"
     CDS             complement(289..966)
                     /gene="nth"
                     /locus_tag="KMT45_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBE7005730.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(973..1671)
                     /locus_tag="KMT45_002285"
     CDS             complement(973..1671)
                     /locus_tag="KMT45_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBE7005731.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1939..3123
                     /locus_tag="KMT45_002286"
     CDS             1939..3123
                     /locus_tag="KMT45_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBE7005732.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3129..3572)
                     /locus_tag="KMT45_002287"
     CDS             complement(3129..3572)
                     /locus_tag="KMT45_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE7005733.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3798..4085)
                     /locus_tag="KMT45_002288"
     CDS             complement(3798..4085)
                     /locus_tag="KMT45_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005734.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4256..5608)
                     /locus_tag="KMT45_002289"
     CDS             complement(4256..5608)
                     /locus_tag="KMT45_002289"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBE7005735.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(5880..6326)
                     /locus_tag="KMT45_002290"
     CDS             complement(5880..6326)
                     /locus_tag="KMT45_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBE7005736.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6562..7488)
                     /gene="cysK"
                     /locus_tag="KMT45_002291"
     CDS             complement(6562..7488)
                     /gene="cysK"
                     /locus_tag="KMT45_002291"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBE7005737.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7628..8420)
                     /locus_tag="KMT45_002292"
                     /pseudo
     CDS             complement(7628..8420)
                     /locus_tag="KMT45_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
BASE COUNT         2286 a         1729 c         1403 g         3002 t
ORIGIN      
        1 acttatcatt tttattacac ctttagatag atacgtcact tatttatata atcctttgga
       61 atactggact tattttatag gtatattgga aggcattatt ttctacatcg ggtattttcc
      121 caataaaaat aacaactttt atttttatcg caataaattg taataagtta ccatcctagc
      181 tactgagtat gtacatttac tttttcatgc aaaaaagata aacgaaacag cttttctcca
      241 taataacaag ataattcata catttatttg caattaaaga aaccacgtct acaggcgtgg
      301 tttctttttt agcgcctttt cttttagacg catccgattt ttcccatctt gacatattga
      361 tagcaaagga catacctcgc acttgggatt tcttgccgtg caatgatatc taccaaaaaa
      421 aatcagtgta tgatgtgtct ttacccataa ttcttgtggt actttccgca tcagtgtttc
      481 ttctacttcc atgaccgtag catctagttt gcagatcctc aggcgttttg agacacgttc
      541 cacgtgggta tctactgcga ttgctggtat cccgaatgca tcccccaata cgacatttgc
      601 tgtctttctg cctacaccag gaagagacat caattcttct cttgacgttg gcacttgccc
      661 atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg ttttttgctt tatttcgata
      721 caaaccaatc gtttttattt taagaatgat ttcatcgatt gaagcttctg ctaacgcatc
      781 gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta ttcactgata catctgtcgc
      841 ctgggcactt aagatcactg caatcaataa ctcaaatgga ttattgtgct tgagttctcc
      901 atgtgcttcg ggaaacattc catacatcgt ttctagagct tccattgttt tttgtttgct
      961 taacatattc cgctactcct ccaaccaatt tttcagtgag actttcggca attcttcttg
     1021 ttgagctgac tgttctactt ctctttgaag aagctgctgt tttcttcgtt tttggtcttc
     1081 ttccacttgt tgttttgtac gtagattttt tctttcccac gatagcaaaa tgcggtcaac
     1141 gtaattaaaa ctataagcct gatttaatac ggcttcccgc agtgctagct tcaaaatttc
     1201 cggctgataa tggtcttctt ctagccattg tccgatccgc tggaactcga tcgaagaaag
     1261 cgggcgtcca aattcttgtt caaataattg atacactgac tggatttctt tttcttgtgt
     1321 tttttcttcg tatttttttt cttgtgtttt taaatactca cctaatagat catagatggg
     1381 gtaaagatta tatcgatctg cttttttacc gttgacaatc gtcgtttcta tcttgataaa
     1441 tccatttgtt acgagacgat ccagtatttg aaaaatctga tcttgtttga ttcccatatc
     1501 catagcaatc atctgcaaat cagggaaagt atctccttca agctgtgcca tatgcaactg
     1561 tagaacaaat aaaaattctt cagattgtag accgatccta tgataatttt taagtaaaag
     1621 attagatatg actgtttgtc cagcgcttaa gtaattttct aaattcaaca tgatttctcc
     1681 tccgctcaat cacagtatac gtaaaaaaaa catgggaagc aagaacaggt ttcttttcta
     1741 tttacttcac tgatcggtca gatttacaat gtcgtctact tgatttttgt ttgttcatgt
     1801 ttttgatgcg tatcttcact tttcggaata aataaattaa gtagatttcg ctgttcctta
     1861 gaatgttgac aaggaaacaa aattactata cactattttt gtttccaagt taacattatt
     1921 gcataaagga gtttttacat gtacagtttc acaaataaac attctaaagt ttttggatac
     1981 gtctctgtct tattcactgg attaggattt actatcgtca gtcctgtact tccgttttta
     2041 acgctgcctt attctcactc catacatcag caagctttct atatcacttt gctcatgtct
     2101 gtttatgctc tagctgcctt cttatctgcc cccattctcg gtagcttaag tgatcatttt
     2161 gggcgtcatc cgatccttat tatcagttta ttaggatcaa gtatcggata tttgcttttt
     2221 ggtttaggaa attcgatatg gatgttgttc cttggtcgta tcattgaagg attgacggca
     2281 ggagaaatct ccaccttata tgcttacttt gccgacatca cggaacctaa tgaaagaacc
     2341 aaagtttttg gatggatggg tgctttagtc ggtataggaa caacgctggg tcctattata
     2401 gggggtctat tagctgagtt ggggaattct gttcctatat ttattggagc actctttact
     2461 ttcttaaatg ccgtttatgg ctacacattt atgctagaaa gccttccaat aaaaaaacgt
     2521 tcagtagatt taagtttctc ccatgtaaga ccttttcacc aattacaaca attattcaag
     2581 atttcatccg ttattccttt attgacagca ggttttgctg tttggttggc agctggctct
     2641 cttcaatcta tcttttctca attttcgatt gacgcctttc agtggaaggc tgggttggtt
     2701 ggtttaagct tctctttgat tggaatcttg gatactgtct cgcaattgtt cattatgcct
     2761 cgattgctca aaaaattttc agaacagcag atcacaagaa tagggatgtt cagtgaaatc
     2821 ctcgcctatc tattcatcac attatctgga attcttctac tgccgatttt gtttttattt
     2881 gggattatct gttatggttt cggtgattct atttttactc ctgtattcaa tggccaacta
     2941 tccaattctg tctcagataa ccagcaaggt cttgtaatgg gcggtaccca aagtatacaa
     3001 tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc agcaccattt
     3061 cttccgacgg tattcggatt tttgcttatg atttttgcct attatcacta caataaaaaa
     3121 tagtagcttc atttgctact tcgtataaat tctctgtatc gaccgataaa acgaaatact
     3181 gtgtcaaatt cttcttttga catattttca aatacagtag catctcgctg tacccatttt
     3241 tgatggagtt gctcgtgtct attccttatt ttttgtccga ttggcgtcaa ttgaaaatag
     3301 atttcttttt tattctcttc gctttgataa gtgtttatga tccctctagc tatcagtttt
     3361 tttgttagct tactgatggc tcctctagtc atgtaactag cattcgctaa ttttgtcacg
     3421 ttaggatcag ggattttccc gatatgttca atcacttcta gttcatttag agtaaatcct
     3481 gcaaattctt tttccatttc tggtttatta cgatgaataa tctcattagc taaatccaga
     3541 tattcttcta ttagcagttg tcttttatcc atttctacac cttctttttc tttcattata
     3601 ttgttttttt gtttcctatt caacaaagat aaaaatattt tgtaggaaaa cccaatctga
     3661 aaccaagaaa aaaaggggtt gacaaagctt gtcataaccc ctttttctga ttaaaagata
     3721 gtaaagagac agtcatcggt aattgtgatg attttttgtc atcacttaag gatgagcggc
     3781 cttttcaaaa actgtcttta aagatcatca tcagtttcta gcacatatgg ctgaccttca
     3841 tatgtgtact ctgtattcaa tgtgccgtca ttttcatctg agtagatgcg tctactgaaa
     3901 ggaaaacgat gagaaacatt atctttggcg tcatttgctg gcatcgtgta atattcctct
     3961 tgcgtttcat tcaactgaga gagtacttga tctgccaact catcaatatt gatcgttaat
     4021 ttcttcgaag cacttttata atcttctaaa aatgcatctt gttggacaaa atattcttta
     4081 ttcatcttct ttctcacctt tcttttcaaa atcaaataaa gctgcttgtt cttttatgta
     4141 acagctttat ctttgctgct tattgtgaca aaaaattcga agaaagacaa ctaattgtac
     4201 ttttcacaaa aaaagacaga gggtcgtgac aaaattgtgt cacaaccctt tttctttatt
     4261 gtgcagtttc gatttcgcct agcgcttctt gtgcaagtag gttcaagtaa ttccatgggc
     4321 ggtcaaaatg tggttggaag aagaaatctg aaagtgcgag atcttctaca gtcattttat
     4381 tttgaacagc taaagacatg gtatttgctg attgtgtaat gtcgtatttg gacatgaact
     4441 gagcacctac gatacgattc gttccttttt catagactag ctccatcatt actttttcag
     4501 tagttggcat gaactctgga cgataattat cttcaaatac agtcgcttgg acttctagat
     4561 cgttcaaagt tgcgctttct ttggtcacac ctgtcgaacc gatcgtccag ccaaataggt
     4621 aaagtcctga agttccttga gtaccacgat acgccatttt atgatctgtc aagttatttc
     4681 ccacaagtaa accttgacga acggcatttg ttgccaaagg aatatagttt tgcgtgccac
     4741 ttggattgta gttgactact gcagagtctc cagctgcaaa aatgtctgga tcacttgtct
     4801 gcatgtattc attcactttg attgccccgt ttggcagtgt atctactttt ccttcgatca
     4861 gttttgtatt tggacggaag cccacacata aaatgaccat atctgtatca aatgtatctg
     4921 ttggtgtagc tacttgtttc acttttcctt cttcatctgt gataaattca gtaacatttt
     4981 cgcctagcgc aagagtaaca ccgcggtctg tcaattcttt ttctaagata tctgtgaatg
     5041 gtttatctag atatttattc aagatgcgat ctaatccatc aatcaatgtc acttgttttc
     5101 cagattcgac aaaagcttct actaattcaa taccgatata tccaccacca acaattacta
     5161 cacgttttgc ttctttcgct tgtgcaataa tttcatttgc ttgattgtag tttttgcata
     5221 ggagtacatt tttcgcatcg attccactaa tcggtgggat aataggccat gaaccagttg
     5281 tcatcactaa tttatcataa gaaaccgttt ctgtttctcc tgttttcaga tcttttgccg
     5341 ttactgtttt attttctgta tcgacgtctg ttacatcgtg ttccatttta actgttgctc
     5401 ctaatgaaga taattcttca ggatttgagt agaacaagcc tgctgggtcc tttactactc
     5461 cgccgacata aagtgcgata ccgcaagata agaaagaaat attgtcattt ctttcatata
     5521 ctgtcacttc tgcttctgga tgatttttta aaatactttt tacagctgca gtacctgcat
     5581 gagtacagcc aattactact actttcattt atggtccctc cgattaacat tttgtccttc
     5641 gacaaaacta ctataacaga aataaaacgg atacaacagt ttaatgcttg tgaattcaca
     5701 aaggtttgtt tgtagtttca ttccttttca agcataagaa cccaatatct acaagattct
     5761 tattgtgata atattcactt tgatatctaa gattgtgaat aaaagacaaa aaaataaaaa
     5821 agctagcgtt tttttacaca gctagctttt tttaggtata acacttatag aaactgtttt
     5881 tacatttgcg ccttttttgc tgactcttta caatccggac agagtccata caattccaac
     5941 cgatgttcat taatctcaaa atcagtcagt ctgctcgcag ccatttctat atcttcaagc
     6001 cctgggtaat gaaaatcaac aatcttaccg cagttttcac aaattacatg ataatgacgc
     6061 tttgaactga aatcaaatcg gctagaagca tcgccgtact tcatttcttg aacaaatcca
     6121 atcgaagtaa ataagcgaag gttattgtaa acagtcgcaa cactcatatt tggaaaccga
     6181 tgttcaagtg ctttataaat ctcatcagcc gttggatggg aatgatgttc aatcaaatat
     6241 tctaaaaccg cgtaccgttg aggtgtgatt cgaatgttct ctttttttaa cttttcgata
     6301 acctcttcga ctaacttatt ttccatgtaa acaccccacg ttgttattct cgtcttatca
     6361 tactattttt acattttaaa atccacttaa catgaaaaga aaattcaatt ttattgtatt
     6421 ttaatgaaaa aagacgtaaa acagtcgcgt tcactatttt acgtctctat tcaaataatt
     6481 tttctcatca taaatttttg tgtgttcttt tcatctttaa aagtttttta cataaaccta
     6541 tctggagaaa aaccaagtgc cttatatctc ttggtaaagg gctgtagata aataacgttc
     6601 accattgtcg ggaaccacag ctaatacctt cttgcctttt cctaattctt ttgcgacttt
     6661 tagcgcggca gcaattgcag cgccagatga gatgcctact aaaatccctt cttttcgccc
     6721 aacttctcta gctgtttcca tcgcttcgtc tccactaata gaaaggactt tatcatacac
     6781 agaagtatcc agtgttttag gaacaaaacc tgtaccgatt ccttggattt tgtgaggtcc
     6841 cgcttctttc ccttctaaaa tagcagattc tgctggttct accccgatta attcgatttt
     6901 cggatacaca cgttttaatt ctcggccagc gccagtgatc gttcctcctg taccgatacc
     6961 tgcaacaaac gcgtccaagc catttactcc aaatgcttga tgtatctcag gaccagtcgt
     7021 cttctcatgc acaagaggat ttgcttcatt ttcaaattgt aatggtaaaa agtagccgtt
     7081 ttcttttgcc aaacgttctg cttctctgat tgatccagaa ataccatccg caccaggagt
     7141 caaaatcaac tctgcgccgt accctttcat caataaacgt ctttcgatac tcatcgtctc
     7201 aggcatcacg atgattactt tatatccttt tgcaactcct accatcgaca atccgattcc
     7261 tgtatttcca gaagtaggtt caatgatagt atcacctggt ttcagtagac catcgtgttc
     7321 tgctttttct atcatactta aagcaatacg atcttttaca cttcctcctg gattaaaaaa
     7381 ctcaagcttt acaaaaacat ctgctgaatc ttctggtacg attttattta atttgacgat
     7441 tggcgttttg ccaatcaatt cggtgactga gttatagata gctgtcatct tcaattcctc
     7501 cttgtctttg ctcttcttac ttatcataat acaatctgtt catttatggc gagtgattcg
     7561 tctttgacta gcaaaaaaaa gacaaagata taccatatat ggtagtttct ttgccttttt
     7621 atctaaatca aacgtttagt tcttgaccaa ctaataaaga ataactggac atagacatgc
     7681 catttttcgc cattagatca tcaacagaca tgccaaattt ttgtgcaata tcccatagcg
     7741 tatcacctga cacgactgta tatgtctgac cattatttgt atttacatct gttgttgaag
     7801 atgatgacgt tgaagtcgtc gtagtagtcg ttgttgttgt acttgtggta gccgaacctg
     7861 ttgatggcgt atcaaaacgg gttaaattat aagtattaat caatgagttc aatttggccc
     7921 cataatttgg atcggtagca taacgacctg tcaagtacgc tgttgcatca taaaaacttg
     7981 ttgtattgct tttccacacg ccagcataat gataatcgcc cgtggaaaaa ctggttgaac
     8041 gaagaacatt cgcatggtct tggaaggatt cccaataaga agaatatttt cggaatggtt
     8101 cattcatcgt tacccattgt ccgttacata ttctttggtt gccatataaa cgacctgtcc
     8161 gttatagctt cctttcacac cgaatagatt ataatttggc gcactagcta atccggaagt
     8221 gccatatgca ctttccaaca acgcttgagc gatcatgact gaagcataca agtcattact
     8281 tgcagcaact tctgacgcag accagccgat ttgatcgata aacgcttgag gatcagcaac
     8341 tgtttgagat ccttgttcat ctgcatgaac atgttgcggt accgctgtaa ccatcatcgg
     8401 cccaactaaa agactcgttc
//
LOCUS       DAEGIQ010000105         8395 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000105 DAEGIQ010000000
VERSION     DAEGIQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.107"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(57..350)
                     /locus_tag="KMT45_002293"
     CDS             complement(57..350)
                     /locus_tag="KMT45_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBE7005738.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="KMT45_002294"
     CDS             complement(378..1043)
                     /locus_tag="KMT45_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBE7005739.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="KMT45_002295"
     CDS             complement(1183..1815)
                     /locus_tag="KMT45_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7005740.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="KMT45_002296"
     CDS             complement(1822..2889)
                     /locus_tag="KMT45_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBE7005741.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="KMT45_002297"
     CDS             complement(2886..3617)
                     /locus_tag="KMT45_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBE7005742.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3785..4264)
                     /gene="greA"
                     /locus_tag="KMT45_002298"
     CDS             complement(3785..4264)
                     /gene="greA"
                     /locus_tag="KMT45_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBE7005743.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="KMT45_002299"
     CDS             complement(4400..5575)
                     /gene="mltG"
                     /locus_tag="KMT45_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBE7005744.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5740..6900
                     /locus_tag="KMT45_002300"
     CDS             5740..6900
                     /locus_tag="KMT45_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBE7005745.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6940..7887)
                     /locus_tag="KMT45_002301"
     CDS             complement(6940..7887)
                     /locus_tag="KMT45_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBE7005746.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7920..>8395)
                     /locus_tag="KMT45_002302"
     CDS             complement(7920..>8395)
                     /locus_tag="KMT45_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBE7005747.1"
                     /translation="TPDNTLVRKDVAENTDISNARITIWKSAWEIFKTSWLIGVSPKN
                     VVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGILGIIPFMAFLIKSFVLNVYRIIYN
                     KCIGKDYHFVYCLVILSYALYAALNNELVYENTVGTFVFWLFLGRVNSKNNSSYF"
BASE COUNT         2513 a         1753 c         1309 g         2820 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atccatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacagctc taagtagtct agaatattct ttgatactaa gttatgtgca
      661 atccgtatcc ccatgtctcg ctcagggtgt acgacacgat ctgctccgat cttctctagt
      721 acacgggcat ggtactcatt ttgggctttt gctaaaatat tcggtacgcc catttccttt
      781 accatcagag taactaagat gctggcttgg atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatacctaa tgaacgaagt gtcatctcat cctgtgcatt tgccacaaca
      901 gcatgggtgg ctatattcat atattcattg actcgatctt cattactgtc aatagccagt
      961 acttcttgtc cagcttcgat cagtgtacgg cagatgcttc cgccaaatcg tccaaggcct
     1021 acaattgcaa aattttgttt caccattttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt ccaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttaaacgca tagatagctg cttgagttcg gtcttctacc tctaacttag ctaatatatt
     1261 cgacacatgc gttttgactg ttttcagtgt gataaacaat tcatccgcga tttcctgatt
     1321 acttttccct tcagaaatca gcatcaaaat ttcattttct cgattcgtta attcttcatg
     1381 caatactgga tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttcaggttc
     1441 tagtactcgc tcgccgcgtt gcgtagccct tatagcatca gctatttcat gtgctgtaga
     1501 cgtcttcaac aaatatcctg ctgctcctgc ttcaattgca ggataaactt tttcatcatc
     1561 aataaaactt gtgacgataa tgattttggc ttttggccaa tcttttaaga ttgcttttgt
     1621 tgattcgatg ccatccattt cttccatgac tagatccatt aaaatgacat ccggtcgaag
     1681 tgccatcgcc ttttcatagc cttgacgtcc attttcagct tctccaatca cttcaatgtc
     1741 ttcttggata gataaataag aagagacgcc taagcgtacc atttcatggt catctactaa
     1801 taaaactttt atcatcttta tctatgcctc cttcatcaaa gggaccttgg tctccacgct
     1861 cgtaccttgc cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttggtttca ttcatattaa atcctgtccc
     1981 gtcgtcaatg atacgtaata aaagattgtt atctatttta tgcaaataga cttctaattc
     2041 attagcctta gcatgtctta atgtgtttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagtta attttacatc ttctacctcc cattttagcg aaatctggat
     2161 tttattctgc aattccttca ataactgctc aatgccttgt tttaaacttt tttcttctaa
     2221 attgactgga cgcagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgccacc attttcaact gcttacggaa catttctggt gtctcacttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcatcgc tgcaaataat tgctgtgaaa ctgaatcatg
     2401 aagctcacga gctaatcgat gcctttctaa ttttaaaatc tcttctttgg tttgtccatc
     2461 tacatattga ggacggctag tcataatctg taattcactg gatacttcga tcattttttc
     2521 ttttatttta gtaatttcca tatcaatatc tttgatgtac agatcatccg acgcgcttgg
     2581 gatcggctga tgcaaaagtt tggattcata attgccagaa gaaagtgcac gaagcttttc
     2641 ttcgattttc ccgtattttg ccttttgaat aagtgataac aatagaaata cgatcaatcc
     2701 tacacctaat gaaattgcca tcaaatggaa gataagcgga acgtataata aacgcgcacg
     2761 cagcaattct ccccaccaat gactttggtt actggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcattgctt tggatattct
     2881 tcccatcata ttcgtatcac ctcgacatct ccaaccaaag tattggtaat gatcttcaat
     2941 ctgcgaggat tttcatcata atcactgctg cagattttga tctgttcgtt ttttaaggaa
     3001 aactgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa acgaatagcc
     3061 actcccaggg gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagtgt attgcccaaa tcgatgatag tatcaccaga aatgatggca
     3181 atgttgatat catcccattc atagacgtcg tttccaatac gttggttacc aaataattgc
     3241 tgtcttttac gctcattatt atgtgttttt aattgctctg tttggaccat catgatctgt
     3301 ttttttcgcc aaaaagcatt ttttgtcaaa tctattcctg aaatctctac acctttcagc
     3361 ccgataaata aaacggccaa tacaaccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaagctccc taagatcaat tgaaaatttt gaaatttagt acgaggatat
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatta ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagcagtg cttcgactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tttttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttgac tgttggctat aggactttag atgtaagatc gttcagtgaa acctgcaata
     3721 ctcaaagaga aaagatctag agtgcgtcat cagtaagatg aactctctag atctttcagt
     3781 cagactacgc ttgttctact ttgacgattt taactaacat atcgccacct ggtgtagcaa
     3841 tggttacttc atcattttta cgtttgccaa tcaatgcttg cgcgatagga gaatcattgg
     3901 aaatctttcc agaaaacggg tctgcttcag cacttccgac aatcgtgtac tcttcttcat
     3961 ctccatcagg caactcgatg aacgtaactg ttttaccgat cgatacctca tctgcatcta
     4021 cgccgtcatt atcgatgatt tgtgcaaaac ggatcatatt ttcaattgtt gtgattcttc
     4081 cttcaacaaa tgcttgttca tctttagctg attcgtattc ggagttttcg gaaagatctc
     4141 cgaaaccgcg agcgattttg attcgttcaa tgatttctcc gcgtttaact gtttttagtt
     4201 cttctaattc tttttctaat ttttctttac cttctagagt cattggataa actttctcta
     4261 ccatgtgtaa cgtctccttt tttcgataaa ataccgcaaa aggtaatctt ttgaggaata
     4321 tagtccaaaa aagattacca cgcttttctt aaaatgtaaa tgtttttcta aaaatgatca
     4381 atgactagcc cctgtttttc taagaattat ttacatattt ttgcactagt tccatatgtt
     4441 cttcatacgt tttagcgaaa taaacatttc cagtgctcgt atctgctacg aaataataat
     4501 agtcattttc tgctggttct aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag
     4561 gaccaggccc atagccggta ttggtataca aattgtatgg tgaatctaca gctgtatctt
     4621 catacgtcac caattcttta tgttctccta atgcatataa aacagagata tctgactgaa
     4681 gtggcatatt tgccttgatt cggttaaaga agacttgtgc aatattttta cgatcatttt
     4741 cttttacgcc ttctttttca actagagaag ccaaagttaa aacttcttgt acagtcaaat
     4801 ttttctgttt gatagctgaa taatagtttt gcatgaccgt atttgttttg ttcaccatct
     4861 caatcacaag atccttaagc gaagtactcg tgtagtaatc ataagtagct gggaataagt
     4921 agccttccaa acggtatttt gtgttctcag ctttagaggc actttccaac aattctggga
     4981 atttttgata caactcgtta aagaaagtct catcatttac taaatctaaa aattctttct
     5041 tgtctttacc agttaccttc gctacccgct cagcaatctg atcgatgtcg tagccttctg
     5101 gaatggcaat tttcccatct gcaacttttg ttggctcaga tgtgccgcct tcttgcaact
     5161 gcttgccaat ctcatcaaga gtcatattag gcgctaactg ataataacca gcttgaaagc
     5221 cagttaagtt tttaaatttt gtataataat tgaaaacaat cccactttta ataatattgt
     5281 ctttctccaa aatttcgcca atctgcttat tagaagagcc gcttgggatt tccacttgga
     5341 ctaattggtc gtccgattta tctaaaggct tcaacccgct atctacataa cgatatacgg
     5401 taaacccgag aaatccgcca ataatcaata atgtaagagc gatcactaaa atgatcttcc
     5461 tcacgatctt atcttctttt ttacgcagat ttctccgctt gtcgattata tctgctgtag
     5521 ctgccttact ggcttctttt ttgtctttgc ttgggtgttg tttgtttcct gacaaaacaa
     5581 ctcctcctta ctatcatctt cgtaactctg tttattatac atgaaatttc tactgccgtg
     5641 aattatttta ccaaatatta tgtcaaaatt gtaagtggca aatgctcttt ttgaggatta
     5701 caatgaaaaa tagttaaaaa tggataggaa ggatttaata tggcaaaagg aaaacatcaa
     5761 aaaaaacagc agatctctct ttcttttctc gcagttttgt tgctggctgt cagcttcttt
     5821 tcaacaggca tcattacagg atggcaatta acaaaacaac ctcaaccaca aatttataaa
     5881 acggaacccg cacaacttgc ttccaattta acatctaatg agcagatgct tgccagcaaa
     5941 attgatcaat tactgcaaaa atcagattat atcggttcga tttatgtacg caaaaataat
     6001 cgagtgatat tagaaaaagg atatgggtac gcaaatctac gcataaaaga atcgaattct
     6061 ccttctcttt actatcaaat cggttcgatc caaaaagcga tgactgcttt acttatccta
     6121 aaacaaattc aatctggcaa gataactttt gacacaaagc tatctgaatt ttatcctcag
     6181 attcctggaa gtcagcaaat ctcaatcaaa aatatgcttt acatgcgctc tggtcttcat
     6241 cgaaccgctt ctccaaaaaa acctatgtca gatagtgaga tcattcagtt tgcccttcat
     6301 catttgaaat ttactggtta tgatacgtat cattatgaac cattaaatta tacattgctt
     6361 acaggggtat tgatcaaact aatgaatcag ccttatgaaa ctcttttaaa aaaagaaatc
     6421 attcggccac tccatctaga acatacagat ttttatgaaa atgtaaaaaa ttccccgcag
     6481 catgctgtat cttaccaaat gtctgcaact gacgattatt caaaagcatt ggtcgagcct
     6541 gaaacagaca tccgaaatga attaggcaca ggaaatataa gtatgtctgt ttatgacttg
     6601 aacaaattct ttaccagtgt tctgtctggc gatattatac caaaagaact acttttctca
     6661 ttgtggcaaa ataatggtga taaacatcct tacagcggcg gtgtttacag tggaaatgat
     6721 tatatccttg ctcagggaaa tatcaatcga ttccatgcag tagcggcgat gaaaaaagac
     6781 acaaaagatg caatcgtcat ggaaagtaat atccaatctg ataaaaacat caaattgccg
     6841 gccactgatt taagaaatca gatttacgaa ctgatggaag gtgttaattt aaaaagctaa
     6901 gtcgagacaa aaaaaccagc aggatgctgg ttttttgttt caatcgattt ctgtctttaa
     6961 ataacgttcc attccgttca atatatcaaa tttgggttga taacctaaag aacggatctt
     7021 tgaaatatcc gctaaagaat cacgaatatc ccctgaacgc tccgcttcat atttaagcgc
     7081 tagctctgtt cctaagattt gatcaattga atgaatcaat tcaagtagcg tagtgctttt
     7141 tcctgtaccg acattgaatt gctgtcctaa agctttttct tccttagcaa caagtaaaag
     7201 tgcttgtata acatcttcta tataaacgaa gtctctagat tggctaccat caccaaataa
     7261 agtaaattct gttttttctc ctgccaattg ttttttgtat cggtccacta gaatcgaaat
     7321 aactccgcta tatggagagt taggattttg atttggacca taaacgttaa aaaatcggac
     7381 agcactagtc ggaacatcat ataaatgaca gtaatccaaa acatattgtt ctgctgcaaa
     7441 cttatcgatg gcatatggtg ttaaaggacg gatgaccgat tcttcttttt ttggcaacgt
     7501 tggttcatcg ccataaactg cagctgatga actaaatacc aatcgtttta aaccagattg
     7561 atattttcgt accaattcaa gcaataaaag cgcactgtca aaatttacac gatgagtttc
     7621 tagaggacgt tctacagaat ctgccacact agctacagca gctagatgga aaatataatc
     7681 gaaatggtat tctttcatga ccttttccat caaatttttg tcagcgacat ccccttcaat
     7741 aaacgtgatg tgcttcgatt catccagatt ctcttttttc cccatcgata aatcatcgat
     7801 cacaactaca ttgttttctt ttcctagata attcgcgagc gtcgagccaa tgaagccggc
     7861 gccaccggta atcaagtaat tcaacatttt ttctcctctt ttcaagataa aatttattac
     7921 taaaaatagc tagaattatt tttgctatta actcttccta aaaatagcca aaaaacaaac
     7981 gttcctacag tgttttcata gactaattca ttgttaagag ctgcgtatag tgcataagat
     8041 aaaataacta aacagtaaac aaaatgataa tctttaccaa tgcatttatt atagatgatt
     8101 cgataaacat tcagcacaaa actctttatt aaaaaagcca tgaaaggaat tattcctaga
     8161 atacctgtcg atgttaatac attcaagtat gcattatgaa tggcaattcc tactttcgca
     8221 atatatccat ctggaataat tgactgtgca taagatacta catttttcgg agagactccg
     8281 ataagccaac ttgttttaaa tatttcccat gcacttttcc atatagtaat tctagcatta
     8341 gaaatatctg tattttcagc tacgtctttc ctaacaagag tattatcagg tgttt
//
LOCUS       DAEGIQ010000106         8388 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000106 DAEGIQ010000000
VERSION     DAEGIQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.108"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            29..1183
                     /locus_tag="KMT45_002303"
     CDS             29..1183
                     /locus_tag="KMT45_002303"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBE7005748.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1205..1957)
                     /locus_tag="KMT45_002304"
     CDS             complement(1205..1957)
                     /locus_tag="KMT45_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE7005749.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2321..4987)
                     /locus_tag="KMT45_002305"
     CDS             complement(2321..4987)
                     /locus_tag="KMT45_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBE7005750.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5328..6779
                     /gene="cls"
                     /locus_tag="KMT45_002306"
     CDS             5328..6779
                     /gene="cls"
                     /locus_tag="KMT45_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBE7005751.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(6984..7799)
                     /gene="thiD"
                     /locus_tag="KMT45_002307"
     CDS             complement(6984..7799)
                     /gene="thiD"
                     /locus_tag="KMT45_002307"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBE7005752.1"
                     /translation="MEDQYVQAMTIAGSDGDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            8093..8368
                     /locus_tag="KMT45_002308"
     CDS             8093..8368
                     /locus_tag="KMT45_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005753.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         2489 a         1726 c         1486 g         2687 t
ORIGIN      
        1 tttttatcat aagtaaagag gtgttgaaat gaaaataggg attgatcgtc tttccttttt
       61 tattcctaat ttatatttag atatgacaga gttggcagaa agccgtgggg atgatcctgc
      121 aaaataccat atagggattg gtcaagacca aatggcagtc aatcgtgcaa atgaagacat
      181 cattacacta ggagcaaatg ctgccagcaa aatcgtaaca gaaaaagacc gtgagctaat
      241 cgacatggtc atcgtcggaa cagaatccgg gattgatcat tcaaaagcga gtgcggtaat
      301 tatccaccat ttactgaaaa tccaatcttt tgctcgttct tttgaagtaa aagaggcttg
      361 ctacggtggc accgcagctt tgcacatggc gaaagaatat gtcaaaaatc atccagaacg
      421 aaaagtacta gtcatagcaa gtgatattgc tcgttacggc ttggcaagcg gtggtgaagt
      481 gacgcaaggt gtcggtgctg ttgcgatgat gatcactcaa aacccgcgta ttttatcgat
      541 tgaagacgac agcgtatttc tgacagaaga tatctatgat ttctggcgtc cagattatag
      601 cgaatttcct gttgttgatg gtcctttatc taattctacg tatatcgaat cattccaaaa
      661 agtttggaat cgacataaag aattgtcggg tcgaggactc gaagattatc aggcgattgc
      721 tttccatatt ccgtatacta agatgggaaa aaaggcattg caaagcgtat tagaccaaac
      781 agacgaagaa aatcaggaac gtcttatggc tcgctatgaa gaaagcatcc gttacagccg
      841 acgaatcggt aatctttaca ctggttcatt atacctgggg ctaacttctc tactggaaaa
      901 ttcgaaatca ctacagccag gagatcgcat cggtctattc agctacggtt ctggtgcagt
      961 aagtgaattt tttacaggtt atctggaaga aaactatcaa gaatatctct tcgcacaatc
     1021 tcatcaagag atgctagatt ctcgaacacg gatcactgtt gacgaatatg aaacgatttt
     1081 cagtgagacg cttcctgaac acggagaatg tgcagaatat acttcagatg tacctttttc
     1141 gattacaaag atcgaaaatg atattcgtta ctacaaaata taataaaatc ataaaaagaa
     1201 cccttcacga gtcgtgaaag gttctttttt tatctgcgga ccaaaggagc agtctatagg
     1261 tcagcttagt cgtactaagg atttctcgtt tttcactgaa tagtttgcga atgtacctgg
     1321 tcacaacagg atcttttgcg tacgcgtcat agtatagctt catcaattct tcagtaattg
     1381 attcctgtgt aaaataagtg aaatcttttt tcttcacttt gaatccttca tcaattaagt
     1441 atgtttcaaa ttgatccatt acaactggcg atgtattagg atcttcttct atcattccta
     1501 gttgcttttc gataggataa aaaacatcat cgattgattc cactactctg attatcacgc
     1561 accatttgct tgactttttc atcaattttt ctaattgaat aagatgtacc aatgctggat
     1621 ttgctgctgt aaaaaccatt tcatatgtct catttgattg gataaattct tctaacgact
     1681 gtttgataaa ggaaacattt ggcaactcat ttttttctgc ttcttttttt gcattttcta
     1741 gcattcctga agaaaaatcg atcaaatcat agcgattaat ttcttttgct aaggaaagaa
     1801 atctgccaga tcctccagca acgtctgctg ctgtttcagc aggcaggact ttctccgttt
     1861 tcagataatt ttttacatct tcagcaatag ttacttgcga ttcttgctgg atttcataat
     1921 attcttcagc aaattcatcc cattcttttt cattcatttg atttcctcta attttttgtt
     1981 atatgttttc acttttcgga ataaatcatt ctcctctttt atctattcta tctcttttga
     2041 tgaataataa aatgattggc tttccgtcta tcataaataa aaaaagggac tgtgaccaaa
     2101 gtcgtgtcaa agtccctctt tccgaataaa cagtgtccaa tcagatgacc ctcagtatta
     2161 gctcaaaatg ctgattttac aacttcttcc ttaaacggtg ttctcctgcc aactcttcgg
     2221 tcacaagtca caatattcaa caaacatgag aagctgtttg ttgaaattgc accttgtgac
     2281 tcagaattaa acggcaggct cacaacctcg ttgattcgtt ttatttattt ccttgtactc
     2341 gtacaatgat caatttagtg atttccatca aaacaattgt cacaagtgcg attcccatac
     2401 atgtaaagac ttctgtcaat ccaaatgctg atgggataga gaagacttct ttcagtcctg
     2461 ggatcattgt taagcagtaa agcgcgctac atacaactac agctagtaag acacttctgt
     2521 tagagaagaa ccctgcttgg atagctgttt gtgtgtttga acgtgctggg aatgtttgca
     2581 atgtccgaca ccaaatcaat gttgaaaatg acatggctac accgaattca ggtgaatgct
     2641 gcatgccaag ccattgagca aagataacag caatcccaat cagcacacca cgatacgtta
     2701 cagaaatcaa tgttttgtcc gtaaagattc ctgaatttgg atctcttggc gggcggttca
     2761 tcgtagcagg ttcagctttt tctgtaccta atgcaattgc tggcagtgaa tcattgacta
     2821 aattgatgaa caacagttgt aatgctgtaa atggattgga ccagtcagca actaaggcaa
     2881 agatgatcgc aatgatcgca cctaaatttc ctgcaaatag ataagaaata gctttcttga
     2941 tattatcaaa cacattacgt cctacttcta ctgcgctgac aatagaagca aagttatcat
     3001 ccgtcaatac catcgctgct gcatcttttg ccacatctgt acccgttccc atagcaattc
     3061 cgatgtctgc ttgtttcaat gctggtgcgt catttacacc atcacctgtc atagctgaga
     3121 tatgaccttt attttgccaa gcacgaacga tacgaatttt attctcaggt gaaacacgtg
     3181 cataaacagt gactttttct aaaacttcgt ttagttgatc ttctgtcaat tcatctagtt
     3241 ccgtcccggt caaagccagg tttccttctt caaaaatccc gatttgtttt gcgatagcaa
     3301 cagctgttgt tttgtggtca ccagtaatca tgattgtttt gattcctgca cttcttgctt
     3361 ctgttaccgc ctgagtcact tcagcacgtg gaggatcgat catagcaagc aaaccaacaa
     3421 ggatcaaatc gtcttcatct tcaaatgata aatcaaaatt atcaagtgga cggtaagcaa
     3481 aagctagtac acgtaatgcg cgtgttgaaa aagcttcatt ttgctcttgg aattttttca
     3541 tcaattcatc cgtcatcgga acgacttcac catttagcaa tactttttta ctgcgttgga
     3601 agacgatatc tggtccgcct ttaacaaaca agtaattttt gttatcaatt gtgtgtccag
     3661 tagacattaa ttttcgatct gagtcaaaag gaagttctgc ttgtcttggg tagttgtttc
     3721 ggatttcctg ataaggctgg tttaattttt cactaaagtc gataaacgct acttccgtag
     3781 ggtcgcccaa tttagtaccg tcttcactga tcgatgcatc attcgctaaa acactgattt
     3841 cgatcaagcg acgttcgtca gctgtccatt ttgaagggtc atttgtgaaa tccccagtat
     3901 cgccattacc taaaaagtag tcaacaactg tcattttgtt ttgtgtcaat gtaccagtct
     3961 tatctgtaca gatgatactt gtcgaaccaa gtgtttcaac tgctggaagc ttccgaataa
     4021 tggcatgacg ttttgccatt ttattcgttc ccaaagataa cacgattgtt acgatagact
     4081 gtaatgcttc tggaatagca gctacggcta cggctacggc aaacatcact gcgctgacga
     4141 tatctgcagt catatctcct gtaccttctc caagataaat acgaaacagt tggataccta
     4201 aaatcaataa agacaatgcc aaaatagcca aacttagttt tttactgaat ttatccagac
     4261 ctcgttgtaa cggagtgact tgctcagttg ttgattccaa aaggccagca acttgtccga
     4321 tctccgtatt attcccagtt gaagtcacta agaatttacc gcgtccgtaa gttacgatcg
     4381 ttccactgaa taccatattt gtacgatctc cgattggtac cgtacttgtg atatccgtta
     4441 cttctttttc tgcagaagtc gactctcctg tcagcatacc ttcatctact tttaatgatc
     4501 ccgcatctag taaacgtcca tcagcaggta cataatcgcc tgcgtctaac aacacgatat
     4561 ctcctaccac aagttcgttt gccggaatcg ttttttcctt accatcacga atcactcgag
     4621 catcgggcgc tgaaaggttt ttcaacgctt ctaaagaacc ttctgctttt tttgtttgga
     4681 caacactgat tatagaattc aacatcaata ctgcaaaaat aactaagaat tcgatgtagt
     4741 cacccatgac catctgaatc agtgcgacaa tcagcaatac gataaccatt gcatctttga
     4801 aagtttctaa gaacaatttc caagttggct tcttcggctt ttcttttagc tggttcaagc
     4861 catctctttc taatttttcc ttcgcttgcg tggtgctcaa tccatcggtt gagctctgcg
     4921 tttgctgcat caacgagtcg atcgtttctt tatagaccgc ttgttgtgct tgttccttgt
     4981 tttccaaatt tcatccccca ttttttgatc ttggccctag tcctgtctga aaaaaaagag
     5041 acctaagaca aatacgcatt tgtcttaggt ctcaccattt catacaatac cagaaaacca
     5101 tttggttaac ttactgttga tattatcgca acaaacgttg ccggttactc ccctaaggag
     5161 atattcaact gtactaatat cctaccttat taacctacaa aaagcaacta ctttagcccg
     5221 atttacctac attttcatga aaggagtgaa aagtatattc accttggtag gcttctttct
     5281 tctatcatgc tatactaatg ttattgtatg taaggaggaa catttttgtg gtatctagta
     5341 ttataaccgc cctttatcta ttaaatgcac ttattgcttt ggtggctatt ttgatcaaac
     5401 cccgagatgt agcagccatt tgggcatggc tcttagtatt gtttgccctt cctggggtgg
     5461 gttttgtatt atatttattt ttcggacgtg gattaacgga taagaaaaaa ttctatctcc
     5521 gacaaagtga cttgaaagaa ttagaaaact ttcagtcttt tagaggagat accattgaac
     5581 attacgatcc tgacatgggc gataaagaca aacagcaatt tgtcgacttc ttctcttcat
     5641 taaatcgtat gccgctgaca agaatgaatt ctgtcactct tctcacagac ggacaagaga
     5701 aattggattc actgcttcaa gatctaaaaa aagccaaaca ttcgatccat atcgaatatt
     5761 acgcatttgt gacagataat atcggccagc aagtcttaca tgttttagaa gaaaaagccg
     5821 cagaaggcgt ggaagttcga atattatatg atgcatttgg ctctcatggc acaaaagcaa
     5881 aagatttcaa tcgtctaatc aaaaatggtg gacatgtcca tacatttgtt acctcacaaa
     5941 aggcattact tcgtttccga ttgaattacc atgatcaccg aaaaatcgtt gtgatcgatg
     6001 gaaagattag ttataccggt ggtttcaata ttgccaatca atatgtaaat acaacaaaaa
     6061 aattcggcta ttggcgcgat acgcatatac ggattttcgg tgccgcttct tctttgctcc
     6121 agcttcgctt cttaacagac tggaacgtct cggtacctga agaaaaaaag gtcggctatc
     6181 atttgaatta tttctttaaa aaagcagatc gagatgaatc taagcttgct gatacatcca
     6241 tccagcttgt ttcaagcgga ccgaataacg aaagggaaca aatcaagctt tcatttatca
     6301 aattgattac ttctgctaaa aaacgtgttt ggatacagac accttacctt gttcctgatg
     6361 aaagtgtcat tgctgcttta aaaatcgcaa ctgcctctgg tgtagatgtg aaaatcatga
     6421 ttcccaacaa accggatcat ccttttattt atcgagcaac acaatattat gctcggcagc
     6481 tgatcaagga aaatgtacaa atccttgtct atgagaacgg cttcctccat gcaaaaacat
     6541 tgataatgga tgatgaaatc tgcatggtag gttcagcaaa tcaagatatt cgtagctacc
     6601 gattgaattt tgaaacaagt gctgtcattt acgatcctga gtttttagaa gaacttgcta
     6661 ctcagttcaa agaagatgag acacattgtt catccatgac aactgaaaca gtcaaggaaa
     6721 tgtctaactg gctattattc aagcaacaaa tttctcgatt attttctcca atcctataat
     6781 ttttttattc aagtagagaa aaatatacta agattaaagg gactgtgaca aaaatcttgt
     6841 cacagtccct tcttccgaac aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
     6901 atattcaaca aacatgagaa gctgtttttt gaacttgttc cttgtgactc ggagctggac
     6961 ggcaggctca cgacctcttt cttttactct aattcaccag ctgcccaatg attgatgggt
     7021 ccatatttgt gccctacatt tatttcttca cgaattgcac ggtttacata gtcttttgcc
     7081 aatcggatcg cttgttcaac tgtttttcct ttcgctaact cagctgcaat acatgcagat
     7141 agcgaatcgc cagttccgtt gatccggtcg gtaggataaa atggtgcatt taaccaaaat
     7201 gattttcctg attcgagcaa tacgtagtca ttgactgttt cttgctgcgc atctttagca
     7261 tgatgtcctt tgatcataac gttttttgca cccatatctt gcagttcttt tgctgcttgc
     7321 acaaaatctt cttcagctgc taatgtccgt ccagtcagct tttctgcttc ataaaaatta
     7381 ggtgtcagta cttcggccaa agggacaagc ttttctttca agctttgcaa agcagattct
     7441 tctagtaaaa gatttccatg tttcgttacg attacgggat ccacgactaa tggtccaaaa
     7501 tcatattttt ggtaattttt tacaactgtc tcaatcaact ttgaatctgc tagcatacct
     7561 gttttagctg cacgtatttg aaagtccttc gcaatcagtt caaattcttt gtcgataaaa
     7621 tctgttggca atgtcacact tgctccgatt ccgtaagaat tacctgcaac acatgcggtc
     7681 atgacagaaa caccgtaaac atttcttgta aagaatgtat gcatatctgc ttgcatccct
     7741 gcgcttccat cgccatctga tcctgcgatc gtcattgctt gtacatactg atcttccatc
     7801 aaatcgattc ctccattttt ataaaaagat ttttcataat gctgttactg tttctatcat
     7861 atgtttttca aagtatccct cagtaaatat accactacct tttcttggta atcaaattta
     7921 tttgaaaaaa catttgtaat aaatcagaaa atagttgtca tttgtttgta ttcgctattc
     7981 taataataaa taaccactat ttgatttata tatatttcag tatccttctt gttttcatat
     8041 aaccaaaggc gctatgattt ccatatgaaa aaaataaagt caggtgaaaa aaatgaaact
     8101 tgaggataaa aaagtcattt tgaagaaaat caaacgacta ccagaccaaa tagatggcta
     8161 tacgatctgt tctttcaaaa aagacaggag tataaaagct tatacgcaaa acacagaaaa
     8221 aggaataatc tatttcctac atgaagaagg ttacgatacc caaaccttta tgtttactga
     8281 aaaaaaagaa tttcataaac agatgaaaaa actgattgaa aaagaatttc caagaagtca
     8341 taaactctat gtgaatcagc aattttaact tctaactgtt ctttatta
//
LOCUS       DAEGIQ010000107         8386 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000107 DAEGIQ010000000
VERSION     DAEGIQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.109"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            6..1193
                     /locus_tag="KMT45_002309"
     CDS             6..1193
                     /locus_tag="KMT45_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBE7005754.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGLGASGVATARLLIAAGIKNLTLVDKQGGL
                     KKQDSTLNKYQRELLDHIHDTNNNVSVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAMVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1174..2478
                     /locus_tag="KMT45_002310"
     CDS             1174..2478
                     /locus_tag="KMT45_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBE7005755.1"
                     /translation="MLKTLNRIKIDFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2504..3517
                     /gene="citC"
                     /locus_tag="KMT45_002311"
     CDS             2504..3517
                     /gene="citC"
                     /locus_tag="KMT45_002311"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBE7005756.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIHAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKIAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3521..3814
                     /gene="citD"
                     /locus_tag="KMT45_002312"
     CDS             3521..3814
                     /gene="citD"
                     /locus_tag="KMT45_002312"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBE7005757.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNPEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3819..4724
                     /gene="citE"
                     /locus_tag="KMT45_002313"
     CDS             3819..4724
                     /gene="citE"
                     /locus_tag="KMT45_002313"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBE7005758.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELVVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DVTEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4717..6276
                     /gene="citF"
                     /locus_tag="KMT45_002314"
     CDS             4717..6276
                     /gene="citF"
                     /locus_tag="KMT45_002314"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBE7005759.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTQITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVSYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEIEEERS"
     gene            6273..6830
                     /gene="citX"
                     /locus_tag="KMT45_002315"
     CDS             6273..6830
                     /gene="citX"
                     /locus_tag="KMT45_002315"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBE7005760.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYPNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKQVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKICARNRT
                     HTVKEMQKKIEQMVAGPLKGALKNA"
     gene            6823..7686
                     /gene="citG"
                     /locus_tag="KMT45_002316"
     CDS             6823..7686
                     /gene="citG"
                     /locus_tag="KMT45_002316"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBE7005761.1"
                     /translation="MHNNIQKIAQNAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFIAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIKKMTKNLLDDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAHQGSQQEKMIDTLLFLSTEVEDTNLIKR
                     SNNRHIFEEYQLLIHPYFELGGAKTAQGKEYLNYLNQQFKKKNWSIGGSADLLILTIF
                     LDRLMGKGWLC"
BASE COUNT         2879 a         1336 c         1705 g         2466 t
ORIGIN      
        1 aaggtatgct tgatgaagtt ttagaaatcc ataaaaaaaa tgtaggtgtt ttacggatgg
       61 aaacaacaga agccgtgcaa gatgatcaag atttagcaaa aatgtacacc ccaggtgtag
      121 cagaactaag tttaatgatt gagaaaaatc atgctttggc aagagaatta acaattagtg
      181 gtaaattagt ggccgttatt acagacgggt cggcggtatt aggtcttgga aatgtaggaa
      241 accaagctgg attaccaatc gtcgaaggga aagcgctgct ttataaaaat ttagcaggtg
      301 tcaatgcgat tccattagcc atcgaacaaa aatcagtaga cgagattgta caaacaattg
      361 tcaatttgca aaatagtttt gcagggatcc atttagaaga tattgcagca ccgaagtgtt
      421 ttgagattga agaaaaatta caagaaaaac tttctattcc tgtgtatcat gacgatcaag
      481 aaggaacagc gatagtcgtt ttagctggat tgatcaatgc tgctaagatc caaaggaaaa
      541 cattaactga acttcgagtt ttgatcaacg gcctgggagc atctggggtt gcaacagcta
      601 gactattaat cgctgctggt atcaaaaatc taacattggt ggataaacaa gggggattaa
      661 agaagcaaga tagtacatta aataaatatc aaagagagtt attggatcat atacatgata
      721 caaataataa tgtaagcgtt ttggaagatg caatcgttgg acaagatgtt tttatcggtc
      781 tttcaatagg aaatgtttta acgcctgaaa tgattggaaa aatgaatgat aatccgatta
      841 tttttgcttt agccaaccca gtaccagaga tcacacccga tttagcaaaa gaatctggtg
      901 caaagctaat cgctacaggt tcttcgaaat atcctaacca agtaaacaat attttagtat
      961 ttcccggatt gttcaaggga ctattactat cgaaagcgaa taaagtcgat ctttcattac
     1021 aacttgaagt agccaaacaa ttagctgcta tggtcgcaga gcctacggta gaaaaattca
     1081 tccctaatgt ttttgatgaa ggagtcgcaa acgtcgtttc acaggcagtt ttggattatt
     1141 tacaaaaact aaaagaaaac gaggataata tacatgctga aaacattaaa tagaattaaa
     1201 attgatttta ttcctttatc tctttatatc attttggtac tcgcatatct tgcaatgacg
     1261 ataagcaata tcgttccaga aaatgatatg ttagtttctt tgggtattat gactgttttt
     1321 gcttatttat tagaagaaat tggtaaaact attcctattt taaaagattt aggtggtaaa
     1381 gtattagttg ttacattttt gcctgcattt ttagtctata aacattggct acctcaaagt
     1441 actgtaactg tggtttctga ttttatgaat aataataatt tcttgatgtt ctttatcaca
     1501 ctactagttg tcggcagtat tgcttcaatg aatcggcaaa cattggttaa agcaacttca
     1561 cgtattatca ttattttaat tttatgcgat gtgattggtt ctttgatagg aacaggtgtg
     1621 ggaatactcc ttgggttatc tgcttggaat gcgtatttct ttatcgttgc gccgattatg
     1681 gctggtggtg ttggggaagg agcgatgcca ttatcgattg gttatgcagc aatattatct
     1741 ggtatgggac aaaatgatat ctttggaaag attctgccgt gtgtgatgtt gggtagttta
     1801 gtcgcagttc tactgtctgg tatgctcaaa aaaattggag agaaataccc acaattaaat
     1861 ggtcagggta atttgatcga tggggatgat gaacatttag agaacttgaa gggttcagct
     1921 aaaccagtca atattgaaaa aacattgtta gcaggcgtgg tagcaataac gatttatttt
     1981 gttgctgtct atgttgagca cttattaaaa tcactccttg gcttttctat tccttcacct
     2041 gtgatcttat tagtctcgct tatgggggct aaaatgcttg gttttatccc taaaaatatc
     2101 gaagaaggtg gaaattcgtt gtatggattc actgttcgtg ggatcacacc tcccttactt
     2161 tttggcgtag gggttgcaaa aacagactgg ttagagctga tcgaagtatt taaaaaccct
     2221 gcaacgatag taacaatcat tgtgacggta gtagctattg tcgctacagc ttttatcgcc
     2281 gcaaaatttt taaaaatgta cccagttgat actgccatcg cagtttcatg ttgtagcgga
     2341 caaggcggta caggtgcttt agctatctta gcggcgggtg atcgcatgga attaatgcca
     2401 tttgcacaag ttgctgttcg actaggtggc gctatgaccg taacgttcgc tatcttttta
     2461 atgggtctgc tatcataaga acataaagaa aggacgtgac catgtgtttg atcaaacaga
     2521 tatccaacga ttatttttaa aaaataacta tatcaaagac caatgggaaa atcttttgct
     2581 tgattcagga atacaaaaga agcaaatcga agattttcaa catttagatc aaacattggg
     2641 catataccat aaagaaaagt tagtaggaac agtatcttat caaaacaacg tgattaaata
     2701 tatcgccgtg tctgaaaaat ataaagatgg tggacataca tttaatgtac tagtctctgc
     2761 aataattcat gcaatgagtc aacaagagat ttttcattat attgttttca cgaaaccgcg
     2821 ctatcaaaaa agttttaact ttttaggttt tgaaacaatc gtatctaccc caaaaatagc
     2881 tgtattagaa atgggagata gttctataaa acagtaccta gagaggattc ctaaagtgaa
     2941 tggtgaaaaa atagcagcaa ttgtcatgaa tgcgaaccca tttacagaag ggcattatta
     3001 tttagtaaaa caagctgcca ataacaacga ttgggtatat gtatttgtac tcgaaaaaga
     3061 acaaaatctt ttttcgacga aggaccgact caaattagtt caacaaggat tacaaaaact
     3121 gaaaaatgtc attgttctat caggtggcga gtatattatt agccctgcaa cgtttcccac
     3181 ctatttttta cgagaagatg atgaaatagc aaaggagcaa atggtcgtag atgctacttt
     3241 attcaaagaa agaattgcca cttatttaga tatctctgct agatatgtgg gagaagaacc
     3301 actttcccca atgactcgtt catataacga catactggaa acgattttac cccctgaaat
     3361 aaaagttcat gtccttgcaa ggaaaaaaac ggaacaacac caagttattt ctgcttctca
     3421 agttcgtaaa gcgtatcttg ctgggcaatt ggaaaaaata aaatatatgg ttccagaaac
     3481 aacctatcaa tacttaaaaa ataaaaggga gcgttaagta atggaaataa ataaatcagc
     3541 gattgctgga acattagaat caagtgatgt ccaaatcatg atttcaccga atccagaagg
     3601 aatagaaatc gatttagaca gtcaagtgat tgagcaattt ggaaaacaaa tcaaaaaagt
     3661 tatctatgac acactagcag tttatcagat taaaaacgta aaactcaagg tagtagataa
     3721 aggggcatta gattgcgtta ttgttgcacg attaaaaaca gcgatccatc gttccttagg
     3781 tatccaacac caagaaacac cgtgggaggt ttaattagat gatgaaagaa cgtttgagaa
     3841 gaacaatgat gtttgttcca ggaaataatc cagcgatgat caaagatgca ggtatttatg
     3901 gtgctgactc gattatgttc gacctggaag atgcagtgtc tatggcagaa aaagacagcg
     3961 caagaatgct tgtggctgag gcattgaaaa cagtggatta tggtgacaca gaattagttg
     4021 ttcgaataaa tgacctgcac tcagaatttg gacgtgcaga catttttgca atggttaaag
     4081 ccggcgcgca tgtattacgt ttacctaaaa cagaaacctc tgaagatgta acagaagtag
     4141 aaaaagtgat tatagaagca gaaaactatc ataatgttcc gatagggcga acattgatga
     4201 tggcagccat tgaaggtgct tctggtgtat tgaatgcacc tgcgatcgcc aaagcatctg
     4261 atcgattgat tggaattgcc attggggcag aagactatgt gacaaatatg aaaaccaaac
     4321 gatatccaga tggaaacgaa ttgttttttg ctcgcagtat gatcgttcat gctgcacgtg
     4381 cagcaggcat ttccgcattt gacactgttt actctgatgt ggatgatatg gaaggatttt
     4441 taaacgaagt acgtttaatc catcaattag gttttgatgg aaaatcttgt atcaatccac
     4501 ggcaaattcc agtagtcaat actgtttatc agccgtcaat caaagaaatc aaacgatcac
     4561 tagacatcat ctcggcaatc gaagaagcaa aagaaaaagg ttctggcgta atttctttaa
     4621 atggaaaaat gatcgataaa ccaattgtag aacgtgcaca acggacattg atgttagcaa
     4681 aagcatcacg attgattgat gaggagggaa atatccatgc ataataaaca aagacgtagc
     4741 attccatcaa gtgttttaac aaagtataaa aaagaattcc aaagtgtttc attatcgcca
     4801 caaacgattc aacgttgtgc accaaaagta acgactgtaa ctaatcagac aaataaattg
     4861 atgcactcga tcaaagaagc tatagaaaaa tcgggcttaa aagatggaat gacgatttca
     4921 tttcatcatc atttaagaga aggtgatttt gtttttaacc aagtgatggc cgtgatccgt
     4981 gaaatgggca tcaaagagtt gaccttagca ccaagttctt taacgaacac tatgaatgag
     5041 atggttattg aatgcatcaa ggacggtaca gtcactcaga tcacaagcag tgggatgcgc
     5101 ggcagcttgg gtgaatttat ttctcatgga ggaatggaca atcctgtgat tttacgttct
     5161 catggtaatc gtccacgtgc aattgaaagt ggtgaattga aaatcgatgt tgcatttcta
     5221 ggagtaccaa catctgatga ttatggaaat gctaatgcga ctgttggaaa atccgttttt
     5281 ggttcattag ggtatgcaaa agtagatgca caatatgcag ataaagtcat tcttctaact
     5341 gatcatttag tttcttaccc taatacgccc attagtattc cgcaaacaca agtcgactat
     5401 gtcgtggaag tagaagcaat tggtgatccg aataaaatcg gtgctggtgc tacacggttt
     5461 actaaaaacc caaaagaatt aaaaattgct gaaatggtca aagatgtcat tgttcactca
     5521 ccttatttca aagatggctt ttcttttcaa acaggaacgg gtggtgcagc tttagcagta
     5581 acgcgttact tagctagtga aatggaagaa aaacagatca aagcttcttt tgcattaggt
     5641 ggcatcacta aaccaacggt tgatttattg gaaaaaggtc ttgttgaaaa agtgttagat
     5701 gtccaagact ttgacaaagg agcagctgaa ggaatggcgc ggcttgagaa ccaacaggaa
     5761 atcgatgctt ccttttatgc tgatccaagt aataaaggag cggttgttaa tcaattagat
     5821 gtcgctattt tatctgcatt agaaattgac acaaacttca atgtaaatgt tatgactggt
     5881 tcagatggtg tgctaagagg tgcgattggt ggacatcaag atgcagcaac ggctaaactt
     5941 actatcatct cagcgccgtt agtacgtggc cgaataccta cagtagtaaa tgatgtcact
     6001 acgttgatca caccagggaa aagtatagat gtattagtca cagaagtagg ggttgctatc
     6061 aatccccaac gtaaagatct aatagctatt tttgaaagaa ttccacaaat tcctgttttc
     6121 acaatagaag aattacagca aaaagcagaa aaaatagtag gcatccccga acctttgcaa
     6181 ttcacagatc ggatagttgc ctatgtagaa taccgagatg gttcaatttt agatgtcgtt
     6241 cgtcaggtga aagaaattga agaagagaga tcatgatgtt tgatactgga gaaattcaag
     6301 aaatcgagga tattttgatc cataaagaac aacgcgctaa cttgcaaagc aacctgcttt
     6361 ccaagtatcc aaatagttca ttgatggtct ttacattgaa tattcctggg ccgatcaaaa
     6421 ataatccaat gattgagcag gtatttcgtc agggacagca gtcaattcag caaatgtttt
     6481 taaaaaaaca ggtggaaatg acgtacacaa aagaaatcaa tttgccaagt ggattggact
     6541 ggtacgtaat agtaacagaa gcacctttga atctcaaaaa atggttggtc acgttagaag
     6601 agcaagacgc attaggtaga cttttcgatt tagatgtctt gtttaaaaaa ggagagcaga
     6661 taacaattgt tagtcgccaa gctcttaatc atggggaacg tccttgtttt atttgtggga
     6721 aatcagcaaa gatatgtgca cgtaatcgta cacatactgt gaaagaaatg caaaagaaaa
     6781 tcgaacaaat ggtggccggt ccattaaaag gagcattgaa aaatgcataa taatattcaa
     6841 aaaatagcac aaaatgcaca attagcgatg ctttatgaag ttacatgtat caataaacca
     6901 ggattggttg atccagtaga tcctggatct caccaagata tggatatttt tacttttcta
     6961 caaagtagtg tagttttgac tccctatttt gaagaattta tccaaacagg attggaccga
     7021 caacatcaac caattgaaga aacctttata gcaattcgac agattggttt agaagctgaa
     7081 actgcgatgt tctcagtgac aaacggcata aatacacata aaggtgctat cttttcattg
     7141 gggatttttc tggctatttg tggccggtta actatttgga aagttccttg taaaaaaagt
     7201 ttctttcaaa aagaaattaa aaaaatgacg aaaaatctgc ttgacgattt tgggaagatt
     7261 gatcaaacaa aaccaaaagc atggacttgg ggagaatatc tatttgttaa tcatggtttg
     7321 acagggatac gaggggaggc caaaagaggt tatccttgtg tttttgaccg aggattacct
     7381 tattatcaag cacatcaagg atcgcaacaa gagaagatga tagacacttt actttttcta
     7441 tcaacggaag tagaagatac caacttgatc aaacgaagta ataatagaca tatttttgaa
     7501 gagtatcaat tacttatcca tccttatttt gaattagggg gtgcaaaaac cgcacaagga
     7561 aaagaatatt tgaattactt aaatcagcaa tttaaaaaga aaaattggag tattggtgga
     7621 agtgcggact tattgatcct tacaatcttt ttagatagac tcatgggcaa agggtggtta
     7681 tgttgatgtt aagatgcagt attttgtatt agcaggctaa caactgaaac aatgggtcaa
     7741 cacaaatatt ttacaaaaaa gggtagtgtc tatcctttca gactgtagac aaagaaaaat
     7801 ttctttgtct acaattcttt tgttacaatt aaatttttag cggagtgtat aaaggcatcc
     7861 aaacagttaa tgatagtaaa taatagaaag gaggaagtca gaattatact agatgcgctc
     7921 atttctaaaa atgattttgt acataaaatt ggtcatccat tgtctccaga ttctatttac
     7981 cgatcggtga actagccaat tcgactattg atcctgacat atagatgtag atgctattca
     8041 atcaatagtt acttgaagtt agttataaca ataaaatctc ctcataaata attccaatca
     8101 catttttata gcatcttcta cagattttta aagagggtaa taaacaaaca gaatcaaaat
     8161 ttctaggact gatagagtgt tctatcagtc ctagattttt ttataaaaaa ttgcaggaca
     8221 atctttatgt attgataaaa acgatttgaa cgtgaacatc tagatttctt cactttatag
     8281 cagtttagct tttgtgagta actacatttc caacgacttt ttatattcta cttaacaagt
     8341 cattaattgc gtacattaca attaaaataa tcaacatgat tgaaaa
//
LOCUS       DAEGIQ010000108         8270 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000108 DAEGIQ010000000
VERSION     DAEGIQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.110"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     regulatory      122..201
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            199..1392
                     /locus_tag="KMT45_002317"
     CDS             199..1392
                     /locus_tag="KMT45_002317"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBE7005762.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1522..2994
                     /locus_tag="KMT45_002318"
     CDS             1522..2994
                     /locus_tag="KMT45_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7005763.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3077..3865
                     /locus_tag="KMT45_002319"
     CDS             3077..3865
                     /locus_tag="KMT45_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBE7005764.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3932..4600)
                     /locus_tag="KMT45_002320"
     CDS             complement(3932..4600)
                     /locus_tag="KMT45_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBE7005765.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4715..6427
                     /locus_tag="KMT45_002321"
     CDS             4715..6427
                     /locus_tag="KMT45_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005766.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6430..8202
                     /locus_tag="KMT45_002322"
     CDS             6430..8202
                     /locus_tag="KMT45_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7005767.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2530 a         1385 c         1817 g         2538 t
ORIGIN      
        1 ttatctttat ggatgattgt gctaagcttt tttggacagt tctaatgtaa aatattttta
       61 tattttttaa gcaaaaaaat gaaaaaatac gagcagaaaa ctgttttatc tgctatactt
      121 ggttcgttgc aagttcccga aaggatccgc aggacggaga tgccttgtaa ccgaatttaa
      181 ctagggggaa tcattataat ggtagaaaga cacttattta catcagaatc tgtttctgaa
      241 ggacatccag ataaaattgc tgaccaaatc agtgatgcaa tcttggatgc aattttaaaa
      301 caagatccaa cagcacgagt agcgtgtgaa acgtctgtaa caactggtct tgttttagta
      361 tttggagaaa tatctacgac ggcatatgta gatattcaaa aagtcgtacg ggaaacaata
      421 aaagaaattg gttatacacg tgcaaaattc ggatttgatg gagatacagc agctgtattg
      481 gttgcgattg atgaacaatc tcctgatatt gctcaaggag ttgatgaagc acttgagatc
      541 cgggatgaag ataaaaaaga cgtattagat gagattggtg ctggtgacca aggtttaatg
      601 tttgggtttg ctgtagatga aacaccagaa ttgatgcctt taccaatcgc tttgagtcat
      661 cgtctggtac gacgcttggc agatttgcgt aaatcaaatg aattaacgta tttacgtcca
      721 gatgcaaaat ctcaagtaac ggttgaatat gatgatcaag gacaaccgga acgcgtagat
      781 acaatcgtta tttcaacaca gcatgatgat gcagtagata atgaaacaat tcgtcatgat
      841 gtcatcgaaa aagtagtgaa ggaagttatt ccagctgaat tattagatga tcaaacgaaa
      901 tattatatca atccgactgg ccgatttgtc attggtggtc ctcaagggga tgccggatta
      961 acaggaagaa aaatcattgt tgatacgtat ggcggttatg ctcgtcatgg tggcggtgct
     1021 ttttctggta aagatgcgac aaaagttgac cgttctgcca gctatgctgc acgttatatt
     1081 gctaaaaaca ttgttgcagc agggcttgct cgcaaagcag aagtacagct agcttatgcg
     1141 attggtgttg ctcaacctgt ttcgatctca atcaatactt tcggtactgg aacggttccg
     1201 gaagaagaac tgattgctgc agtgagagaa aacttcgatc ttcggccagc aggaatcatc
     1261 gagatgcttg atttgcgtcg tccgatttac aaacaaacag ctgcttacgg tcattttggt
     1321 cgtacagatg tagatttgcc ttgggaacat acggacaaag tagatgcgtt gaaagcaagt
     1381 ttagcaaaat aagttatatt cagctggaac gatcgccaag tggtggttgt tccagctttt
     1441 tttatggcag ttaacctctt gaaaaagata gtaattattt atctattttt agatcctgtc
     1501 ataatgaaag ggaggaataa gatgaaaaaa gaaacaaatg ttactttggt cacagtgagt
     1561 attttcattg caacgttcat gacggctatt gaaggtacaa tcgtaacaac cgctatgcca
     1621 acgattgtcg gttcattaca tggaatggaa atcatgaact gggtattttc gatttatttg
     1681 ctgacaaacg ctatgcttac tccgatttac ggaaaacttg cggataaagt gggacggaaa
     1741 ccaatcttta tttttgggac attattgttt attatcggtt cgttattttg cggtttatct
     1801 caaagcatga ttgggttgat tgctgctcgt gcgattcaag ggatgggtgc tggggcaatg
     1861 atgccagtag ctttgacgat catcggcgat ctttattcta ttgaaaagcg agcaaaagtt
     1921 ttaggtttga atagttcagc ctgggggatt gccagtgttt ttggaccgct tgcgggagga
     1981 ttcatcgttg aaacgatcag ctggcactgg atctttttta tcaatgtgcc gatcggaatt
     2041 ctattaatta ttttgatttg gctatattta aatgaaccaa aaatcataca tgagtctaaa
     2101 ccaatggata tttttggtag ttttagtttg atggccatgc ttgtctcatt gctgttaggg
     2161 ttccaattat taggagacga aggaatgagt ccgcttgttt ctgctttgct gctaggtagt
     2221 atcgtaatgt taggtatttt tgtttttgcc gaaaaaagag ctaaagatcc tgtcatcatg
     2281 atgcatttat tcaaaaatcc actatttgtt gtggtcaact tggctgcagc attggctagt
     2341 ggttttttga tgggaatcga tgtttatatc cctatgtgga tgcaaggtgt attagggctg
     2401 aatgcagcaa ttggtgggct ggtattagca ccattatcac tagtctggat ggtcggttct
     2461 ttccttgcaa gcaagtggat gactcaaatg aaaactcatt gggtcttgag aattggacta
     2521 ctaatcgcag ctattggggg cgcttggttg tatcttatgc ctttcagaag taactattta
     2581 tggttttttg ctatatcaac ggtcatcggt ataggcttag gtgtgaccat gactacctcg
     2641 actgtcagtg cacaaaatag tgtatctcgt gaccagcttg gtgtagcaac ttcttttaat
     2701 acgttagttc gaacaattgg tcaaacagtc atggtttcta tttttggttt attgataaat
     2761 ggggtaacta aacgagaact tgcagcagca aatttaacaa atgatgcaga tatcatgaat
     2821 aaactggtga atccacaaac ggctaaatat attgatgcgg atattttgtt accattaaga
     2881 aaaattcttt atgatgggtt acattccgtt tatctagttg gcttgttact cgtgatcatt
     2941 gctttggtct taacgctttt cgttcatgaa aaaagtggta aaataaaaca gtagtttcgg
     3001 ttaagatatg tacgtagccg aatcattcaa atttaaagtt attcaaaaat aacttcttac
     3061 taaggaaggc gaaaatatgg catttattca agcaaatatt tattcgaatg tgttagaaat
     3121 ggaagtcaat ctaaatgtga tactaccgca aacaacaaaa aaggtgattg gcaccagttc
     3181 agagcaagag acaacggata tacctgtttt atatttactt catggaatgg gtggcaatca
     3241 tagtgtatgg gaaagacgta cgtcgattga acgttatgta gcaaactacg gattagctgt
     3301 catcatgcca tctactgatc ttggctggta cacagataca gtttatgaca tgaagtattg
     3361 gacattcatt gcagaagagc tacctgagat ttgtcatgaa ttattcccgc aattaacaag
     3421 aaaaagagaa aaaacatatg ccgctggatt atcgatgggc ggatatggcg cgttaaaact
     3481 aggccttgca aaatcagaaa atttcgcagc agtaatctct ctttcaggcg ctgtttctgt
     3541 aggaaatcga atggatgatc ttttgatggt tcgtaaagca tctttttggg aagggatctt
     3601 tggaccattg gatcaaatcg aaggttcaaa aaatgatccg caatacttat tgaaagaact
     3661 agtaaaaagc ggaaaaccag ttccaagatt ttatatggct tgtggggaat ctgattttct
     3721 ttatcaggca aatcaggaaa tggtatctgc tatgcaggaa caaggcttgg cggtcacctt
     3781 tgaacaagga cctggcgatc ataactggat cttctgggac caatggattc aacgtgcatt
     3841 ggcgtggcta atggaagaaa aataaaaaaa gagtcttaaa aagatagctc ctatagagat
     3901 cacaaacata gtgatctcta taggagcttt tttactgctc gttttctctt cttttatttt
     3961 gttggcataa gccttcaata taggtttgtg cactcgttag aaagagtgct ttttcattca
     4021 attctgcatg ggactgcata ctttctttaa atccctctaa ttgattttca actacataat
     4081 tacgcataaa tagaactgtt tctttcaaaa attcatttcc gtccataatt ttttgtcgaa
     4141 gctgctgttc atgctgctga tccattaact gtccaatcaa taaattgtgg agagaggcaa
     4201 tggcctgatg actttgggtg actgaaaatc cttcacgaac aagtaattgg atcatttgct
     4261 ccaaatgttc taatcgactc ggaaaagcgg gcacagtatt gatttctaat tctgccccac
     4321 aagggaaacg tgtataaagg tcatagtaat tttccatata ctgcagcaac tgttcattcc
     4381 aaggcaaagt tggatctgga agcgctaatt cttcttccat tgtttcagcc attttttgca
     4441 agagctgttg tttgtttttg aaataccaat ataaagcagg tgcttgaaca tgaattctgt
     4501 ttgcgatgtt tcgcatggaa agctgcgaaa gagcttgtgt ttcagccagg atatcaaagg
     4561 cggcctgaat gatgattgct tgagaaagct ttatttccat tttatcaacc tcgtgtattg
     4621 cattatcttt ttaaacatgg taaattgtta gtcggtctaa ttttacagtg ttaaattagt
     4681 cctgtcaatt taaacaaagg ggtgtaatca acgaatgaaa ttaatcttac gacatgcgaa
     4741 aaaatataaa gtagctgttt ttatttcctt gctgtccgta gcagtcatgg ttgcagcagc
     4801 attatggcaa cctaagttac tacagcaagt attggaagct attattacag aagataatga
     4861 tgaaatgaaa acaattggta tttacctgat cagtctggct ttgctaggat tagccgcagg
     4921 tgtaacgaac acgattttct ctgcaaaagt agctcaaggc gtaagtgcag atatccgaga
     4981 agaagcattt cgaaagatcc agacattttc atttggaaat atcgaacagt tctctgctgg
     5041 aaatcttgtt gttcgtctaa caaatgatat cacgcaaatc caaaatttag tgatgatttc
     5101 tttgcaatca ctttttcgaa ttccattctt atttattggt gccttcattt tagctatgat
     5161 cacgatgccg caattatggt ggatcatcgt agcgcttatc gttacggtat ttctgattac
     5221 ggcactttca tttacaagga tgggcaagca cttcatgatc attcaaaaac tgattgatcg
     5281 tgtgaatagc attgcaaaag aaaacttact agggattcgt gtagtgaaat cttttgtaca
     5341 agaagaaaac caattgaagt cattcagcaa agtaagtgag gacttaacaa aacacaacat
     5401 catagtcggt acattatttt ctgtgatgat cccgtccttc atgctggctg ccaatttggc
     5461 tgtggttggg gcgatctttt ttgtgagcga tcttgttaaa gatgatccta cgttaattgg
     5521 tggaattgct tcattcatga actacttgat gcagattatg atggcaatca ttattggcgg
     5581 aatgatgatg atgatgactt caagagcagc cgtatcgctg aagcggattg cggaaatttt
     5641 agatgctgaa ccagatctta cttatttaga cgttccagaa caagagttga cgggttctgt
     5701 cacttttgac catgtttctt ttcgttatcc tggtgaggat acagatacgc ttaaagatat
     5761 cagcttttct atcaaacctg gagaaatggt tggtatcgtc ggggcgactg gtgccgggaa
     5821 gtcaacacta gcacaattga tccctcgtct atttgatccg acagaaggca aagttgaagt
     5881 aggtggtgta gatcttcgtc aagtaaatga aaagagttta agacaaacgg tttcctttgt
     5941 tttgcaaaaa gcaattttat tctcaggaac gattgctcaa aacttgcgcc aagggaaaaa
     6001 gaatgcaaca gaaaaagaga tggaacatgc ttcttctatt gctcaagcaa aagaattcat
     6061 cgaaaagtta tctgatcgtt acgaagcacc aatagaagaa cgtagcagta acttttctgg
     6121 tggtcaaaag caacgtttat ccattactcg tggcgttatc ggtaatccaa agatcttgat
     6181 cctagatgac agtacgagtg cactggatgc tcgctctgaa aagctggtcc gagaagcact
     6241 cgatcgagat ttgaagggaa caacgacgat tgtcatcgca caaaaaattg cttctgttgt
     6301 caaagcagac cgcatcttag tattggatga aggcagatta gtcggcgaag gaacccatga
     6361 ggaattggta gctggaaacc gtgtctatca ggaaatcttt gaaactcaaa aaggaacgga
     6421 ggagtaagta tgactgatct aataaaagca agtaaatttt tctatcacta tttgaagcgg
     6481 tacaaactct cctttttctt tatctttatc acagtgatca ttgctaccta tctacaagta
     6541 aaggcacctc aatacatcgg ggaagctttt caagaattag ctaattacgt aggaggcttg
     6601 atgcaaggcg tagacgataa aagcaaattt gtttctgtaa tatggaaact cttgctgttt
     6661 tatgtactag ctagtgcagc caactttatc tatagtattt tgttcacaca agtagtaggg
     6721 aagtcaacga accgaatgcg tatcggtcta ttcaacaaat tggaaaaatt gacgatccgt
     6781 ttctttgatt ctcatcaaga cggtgaaatt ttaagtcgtt ttaccagtga cttagataat
     6841 attcaaaata gtttgaacca agctttgctt caagtcatca cgaatgctgt tttacttgtc
     6901 gggatcttga tcatgatgtt ccgccaaaat gtagagttag cttgggctac aattgcttcc
     6961 actccagtag ccgttttgat agctgtcttg attatccgca aagcacggaa atatgtggat
     7021 cttcagcaag atgaagtggg gaaactaaat ggttatatgg atgaaaaaat cagcggacaa
     7081 cgtgtcatca tcacaaatgg cttacaagaa gaaacgattg aaggattctt agagcataat
     7141 caaagtgttc gcgaagcaac cttcaaagga caagcttatt ctggtctact atttccgatg
     7201 atgcaaggaa tgtcactggt caatacagcg atagttatct ttttcggcgg ctggttagca
     7261 ttaaatggtg atttggaacg ttcggtggca ttaggcttag ttgtgacatt cgtccagtat
     7321 tcacaacaat attaccaacc actgatgcag atttcatctg gatacagcat gatccaatta
     7381 gcgattacag gggcaagacg attgaatgaa atatttgatg aaaaagatga agtcaaacca
     7441 gaaaacggta aagcatttga tggtgttgaa aaaggtcttg cgttgcagca cgtagatttt
     7501 ggttattctc cagaaaaact agtcttgaaa gatgtctcga tcaatgtgga caaaggagaa
     7561 atggttgctc ttgttggtcc aacaggatcg gggaaaacga cgatcatgaa tctgctaaac
     7621 cgtttttacg atgtcaatga tggttcagta gtcatcgatg gaatagatat ccgagattat
     7681 gatcttgatg cattacgtag acatgtagga atcgttttgc aagaatctgt attgttctcc
     7741 ggaacgatca aagacaatat cacattcggc aaaccggacg cttcagatga agaagtcatc
     7801 aatgcagcaa aacaagcgaa tattcatgac ttcatcatga cgttagaaaa aggttatgat
     7861 acagagatat ccgaagaaaa taacttattc agtacaggtc aaaagcagtt gatcagtatt
     7921 gctcgaacga tcatcacaaa tccatcttta ttgattttgg atgaagcaac aagtaatgta
     7981 gacacggtaa cagaagcaag gatccaaaaa gcaatggatg aagcaatcaa aggaagaacc
     8041 agctttgtga ttgctcaccg gctgaaaaca atcttgaatg cagatagaat cgttgtcttg
     8101 cgtgatgggg aagtcattga agaaggcagt catcatgagt tattagcaga aaatggattt
     8161 tatgcggaac tttatcataa tcaatttgta tttgaaagtt aaattaaaag aacctcgtta
     8221 taacggggtt ctttttatat attttatgtt gtttgttttt ataaggggca
//
LOCUS       DAEGIQ010000109         8228 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000109 DAEGIQ010000000
VERSION     DAEGIQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.111"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="KMT45_002323"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="KMT45_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBE7005768.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="KMT45_002324"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="KMT45_002324"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBE7005769.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="KMT45_002325"
     CDS             2591..3376
                     /locus_tag="KMT45_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE7005770.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="KMT45_002326"
     CDS             3389..4240
                     /locus_tag="KMT45_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBE7005771.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="KMT45_002327"
     CDS             complement(4364..4816)
                     /locus_tag="KMT45_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005772.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="KMT45_002328"
     CDS             complement(5281..5649)
                     /locus_tag="KMT45_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBE7005773.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="KMT45_002329"
     CDS             5799..6281
                     /locus_tag="KMT45_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBE7005774.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="KMT45_002330"
     CDS             6318..7328
                     /locus_tag="KMT45_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBE7005775.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="KMT45_002331"
     CDS             complement(7443..8156)
                     /locus_tag="KMT45_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005776.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DAEGIQ010000110         8155 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000110 DAEGIQ010000000
VERSION     DAEGIQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.112"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..170)
                     /locus_tag="KMT45_002332"
     CDS             complement(<1..170)
                     /locus_tag="KMT45_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005777.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCW"
     gene            complement(177..725)
                     /locus_tag="KMT45_002333"
     CDS             complement(177..725)
                     /locus_tag="KMT45_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBE7005778.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(770..1102)
                     /locus_tag="KMT45_002334"
     CDS             complement(770..1102)
                     /locus_tag="KMT45_002334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005779.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1116..1373)
                     /locus_tag="KMT45_002335"
     CDS             complement(1116..1373)
                     /locus_tag="KMT45_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005780.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1568..2110)
                     /locus_tag="KMT45_002336"
     CDS             complement(1568..2110)
                     /locus_tag="KMT45_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005781.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2131..3222)
                     /locus_tag="KMT45_002337"
     CDS             complement(2131..3222)
                     /locus_tag="KMT45_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBE7005782.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3230..5440)
                     /locus_tag="KMT45_002338"
     CDS             complement(3230..5440)
                     /locus_tag="KMT45_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005783.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5456..7984)
                     /locus_tag="KMT45_002339"
     CDS             complement(5456..7984)
                     /locus_tag="KMT45_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE7005784.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
BASE COUNT         2474 a         1792 c         1173 g         2716 t
ORIGIN      
        1 aaccaacaaa catcgacaaa cagtaaatga tataatccat ttccacctcc aagcatattc
       61 aatactggtg aacatgcctt agaaacacga atatagttat ggtttaaata gatattttct
      121 ttaaacattg cttgtttaaa agccatatgc tcttgttcta aacttttcat aaacttttac
      181 tctaatgcac agctagcagc agctgctgca attcctgcta caatatttac cttagctaac
      241 tctttagctg cagcttttcc taattgtttt tctgcaattt ttaataataa atccgcagct
      301 ttttcaaaat taaaagcttt taatgcttca ataaaatctc cctgcgtacc tatagcccaa
      361 gccactcctc ctccaggaag agaattaatg gctacacatc cagcatatcg tttccaatca
      421 actttctcca ttctaggaac aataatatgt tcataaaaga ctcttgctgc tcttccttgt
      481 tcgttatctt gactgattcg ttcacgaata gcaccaaaat cctttatatg gtatacacca
      541 tgttcgtcta aaaaaccaac tgtatcaaaa taaaaagtta attcatctac taatttattc
      601 tttatttcta aatcaactgt attcactttt tctgtggcat acacacttgt tccagtacta
      661 acaaaagtac ttaaacctaa taagaaaaca cctaaaacac caaatatttt aattattctt
      721 ttcataattt ctcctccata aaaaataagt tactttttaa atatcttgtt tatgaattca
      781 ttccccaaag gccttttaag attgatacag cagtccgttc acttctttta gacttgtcat
      841 acaagtcttt taaatgatcc catatcagac tagtttgtct atctagcttc aatccattaa
      901 acgctaatga acggttcatt ttttctatta ctttagataa agctacagaa atttttatac
      961 cttcacaacg cgcatcttgg atttcaataa tagtatcatc aattagtttt tgaatttctt
     1021 gcctatcaac atattcaatc ttagaaagag aatcttttaa agctactaat gattcaacga
     1081 tttctttttt ctcttcagac attttcttcc tccttctagt gacccgtaaa gccatgtaga
     1141 gcagagtcca ttgaaccagt aattacacta tctacaacgc cattccaatt tactctacat
     1201 tttccatttt tcagatttgt cggtccatac ggatagcctt tttgacattc aatgttcgga
     1261 ttataccaga taggattata ccattcagcc gcagaagcag attcagtcga tacaaacaat
     1321 cccacaccaa caaatccaat acacatcaca ctatagccaa ttagtttttt catctcgtat
     1381 ccatctccta aaaaatagaa tagttcttat agttattcat aagacatatt ttttccgttt
     1441 ttgttttatc ctttctgtcc tataacaact tttttcaagt aaagaatgac atagtttttc
     1501 ttaaaaaaac agtctgtttt tttctatttc ttcggtgtat aataaatatg agtgttcatt
     1561 ttttatttta atacgcactc cgtacataat aaatcttgat cgcatcaatc ttttgagctt
     1621 cttgatctac tttcaattga agtaatacaa attgagtaac cttattcatt gtttcttctc
     1681 caaataacaa atattgatcc ggattctcta tatcttgtgt cacctggtaa agtttcccag
     1741 ttgttttcat ttgtacatgc gggtctacat caatactatt gtctttaata caacgatctg
     1801 tcatatagtc cctcacagct tgattgcgtt tataaacatc tgtgaaatta ctccattttc
     1861 tagcaaaatc ttttaagaaa ttttttgtca tttcatctat ttgcatttgt tgcgctgtct
     1921 cgtcttctaa tgaaccaatt tgactgttac tttcagtact ttgaagagtt ccacttgttt
     1981 cttttactgc ttcgattgtt tcttctgtcg ttctcaattg tgttttttgt ttttcagaag
     2041 aaaaccaatg acaaccagta agacctatca aacataacca actaaatact aaaattatcc
     2101 cttttttcac gtttctagtc actgcctttc ttatttcacc ttacgaacca gactacgaat
     2161 ttttgtttga ttgtacgtgc gttgataccg agccataatc cgtccttgct cggcattctg
     2221 ttccacacta gagaaatgtt gtccgccata tgaaacatct gttataattc cggtgtgacc
     2281 atagattcca ggagacaatg caccacctgc ttgccaattc accacatctc ctgcttgtaa
     2341 atcacttggt tttgggttca tgattacttg ccaaccatag gttgtccagt tataatcttc
     2401 ccctattaac atcgcaaaca tgtgtccaga acccattaac tgtgggccac ctaattgaga
     2461 tacataccat gccgtaccac cataacattc cccattattt acggatcgtc caatgatccg
     2521 atccaaaacc ttcgcattgc ctttactttt tcctgcaata agcgaagttt tttttgttgg
     2581 taggctattc caatgcttga tcgcaaaagt atcatattcc gttaagttat aggacgttat
     2641 gatactcatt gctcctcgat aatacggact cccttcggta atacctgctg ccgcatagcc
     2701 tccacgacct agaatcaata aacaacttcc aggatcttta ttgcccacac attgattaaa
     2761 gttgctttgg ctcgccacca taaagccata atagtcataa caagctttta agttcggaaa
     2821 cttcatatag aaaccacctt cagaaccacc aattccacgt gcagaacctt gtgggtaggg
     2881 acaaccagga aaccaggtaa tcccaaaaaa gttcgtatcg tttcttccag attgtgactg
     2941 tccataagct gattcatagc ctaattgaga aagaatcaca ctcggtaata actgatactt
     3001 tacagctgca ccaactgctg cttgcgcatg ttcatctgtg attggaccac gtcctttaat
     3061 ctctgaaaaa atcggtaatc cttcggtata ttccccatta aaagcgacag ctagacctgt
     3121 agcttctcct ccatgctcag aaagttctcc tgaaaaaaag aatggcaata gaaaaaaggg
     3181 aagaataaag aaaagaagac aacctagaca ccctttcttc atttcttcct catcctttct
     3241 tcgataatgg tttttggact gttcgtcctt ttggtaaagt aaaatcagtt cctggttttg
     3301 cgtccaaaat cggcagtgtt ttaggatcct tttctatttt ttccgattgc tctgttggct
     3361 tatctaaaga agaaaatgca ggttgtaacg attttggtgc ggaaatcgac ggtttacttt
     3421 cagacgaacg ttgccccggt tgttgcacag aacgatttcc tgatagagtg gctaccgacg
     3481 aattaggaac agagtattgg ttcaataact gctgcattgc tggataactc ccatttttcg
     3541 cctgattttt cacttggcga atctgttgtg aaagagaacc agcagctgtt aattgtggca
     3601 cgggtacttt tccagaaggc aacggaatcg attgaccaac aaaagcgata cctttattta
     3661 acaatggttt tctttgatta ttcccttgtt gcttactcaa ttgtgccaat gattgtacct
     3721 gttgttttgg tcttactgaa ttcatggacg aatttggaat caaaccattt gaaagaccag
     3781 aattccactc tgttttcgat ttggtagaag acatttcagg ctctacccca tgataacgaa
     3841 catgacgacc actataaggc aaggcttgat ttgaacgccc acctagttca aagggattaa
     3901 ccacttgcct cccttttacc gattcttgtg gtgtacgttt ttgttgaaac ttttgaaatc
     3961 catgtatcgc tgcagaagcg agtggattcg ttgctcgata cacattccct gctaatcctg
     4021 cacctttgat tgccaaattt tgtccagcag ttgatagttg accaggcagt tgtttcattt
     4081 gagacaaaga atgacttgct atgtttttcg tgttgtttat agtttgatct gcagctccta
     4141 accctagttt ctttaataaa tctttccgtt ttacaatgag taaaattgat aggagtgctt
     4201 tgacaaacat atttcctaaa aaaccaaaaa cagtattcat tggaattaaa tcgtctacta
     4261 tgttatagat caaaaatgcc accaaaataa tcacactgta aaaagatttt tgaaacatca
     4321 agcccaaaaa ggactttaag gtattcatga gcatataatc aaatactgga acaaaagaag
     4381 cgagtaatga aaaaggaatc acaagtaata aacacaacac caacatttga aagataaaac
     4441 gaatcatgcc aatcactaca agtggtaatc caatacttag attaagcaat ggcaccatag
     4501 aaccaacgac tgctttataa acagctttag ttggttgaat atagacacga taagccttct
     4561 ttttccctct atcttctcct gcagcagtgg tcacttttgt ctcaatatca gataattctt
     4621 cacttgacgc ttccttggct agaaattctt gtggttcaat ctctgcttga tacaattctt
     4681 ctaaatttgt tgtcccataa tttaacaata tatacggatc aacaacagcc gctttataat
     4741 aaagttggcg cactttttga atggcttcct tttgtgtcgt tggcactaaa acaccattga
     4801 catcttttgt cgccaattca tctagactat ttgtagcaga aaaaataaag ccttctacct
     4861 ccacggacaa atcatttaac cctttggtaa aagcttctcc tttactaggt acgttactac
     4921 catagccaaa ccaaaccatt gaaaaaatga caacgaagag aaatcgaata aacaccattt
     4981 ttgcgtaatg tttccctcta aatatacgaa gagtaaatag atataacaca tacaagacga
     5041 caatccccgc accaatagga tcgtatattt ttgaataaat agtatccgaa aaagtgaaaa
     5101 actggtgaat taactcgttc attgcagctc cttcatagag cttctcaatg acaaaatcaa
     5161 ggccttgcca aatcaattta cttaaactaa agaacatatt cgtcatcatt tttagaaaat
     5221 ctgtgccatt tgccccaaat aacattttgc ctaatgaaaa ttcactatca gataaataag
     5281 aagaaaaggt atcaatatga aatttcccaa tctcatagat cgattgatcc acctgctttt
     5341 ctgccgcata caccaatcca ccaacaaaaa gcagtccgaa tccaatcgtt aaaaaaaaca
     5401 aaacgcacca tttcttttcc ttatgcttta tataagccat tgtttcacct catttctatt
     5461 gcatgattaa cgtatcctgt tcttctactt gagagtgact tgttttttcc gttgtttgta
     5521 atgcagcggt catttcttca aacaaactgt gtacattgag ttttcctaca cgtccatagg
     5581 gatctaacat ccaacactgt cctttaatca tgtggctcat ttcagctaag ttcgcttcat
     5641 ttacttccaa acgtaaatga ttcaaaatct tttctcgctc actgtcttca tcaaaagcaa
     5701 agacgacacc aaaattccct ttttctttat ctgaacctaa gtcaccaata aattgtgtat
     5761 ctaacactaa ttgattcatc tgggaacgcc ccacacgtgc cattgctgca ataatactac
     5821 gtcctgcgtt cgaattatta aatacccatg cttctgtaaa ataaatcgct gttttcttac
     5881 tactatctcg ttttccaaac atttcacaaa aacgacctaa actaatcatc aaacacaaag
     5941 actttcgatc cgcatctgta tatagttctg gtcggacatt gtcatttggt aatttcaaat
     6001 cttggatctc taaaatcgtt gttttggcat taaagtctaa tcccgggttt tctccataag
     6061 aaaatcctaa acgtaaaatc gaatcttcta ccgtcaagcg taaaaactca ccatattctc
     6121 gaacctcttt ttgttccatt tgttctaaat agtcgaccac atgcatcaag ccaacttgtt
     6181 gtccggcttc tttttgaact aaaacttctt ggattccctt taataccgcc ccttttaaaa
     6241 ggtgattggt acctaacgga cttaactcat caatcatatc ctgggctact tgtttcgcat
     6301 ctactcggga taaacaaaca attggatcta atacaccata attacgggga tttgtagcat
     6361 ctaacgtgac ataactcatt ttttgcaaat gttctacaaa caaagggtaa tgtttctgat
     6421 aatacgggtc ttgaatgagt ttcgtaaacc atttgcgctt ttctcctttg ggatccacaa
     6481 ataaactttg tacattcata aacgaactat acaaaaagag caagccaact aaaaaggtct
     6541 tccctttacc ggtttttcca gttactgcta catgaggcgc atcaaacaac gcacctttga
     6601 ttccttccgc aatggctaga agattcaaaa agacaaaacg atttgacgca taaattaatt
     6661 cttctaatgg tgttttttct cctcgagaac caccatcccc taatgtatct tgccgaccaa
     6721 tataccagcc ggattccata cctaattctg atactgctga aaacaaccat tcgccaactg
     6781 cttctacagt tgttcggtgc ttccaatgta actgccgtcc caatggttgc cctaataaac
     6841 atttatagaa caattctcgt tgatcatttt ttgcacggtg gatatggata atcgtatgaa
     6901 gttgttgaat aaccgttgca ctattttgac gaacttcttc aaccgtttga ccgtatacaa
     6961 taaaacatgc gatccaatcg acgacaggtt gcccattatc tactcgttgc tttaaatgtt
     7021 ttactaatcc tcgaacagtt aataagcgtt cactaggttc ttcatcattt tgaaccaatt
     7081 ccttatgctc tgttttgaac cgttccgata gccaattcag ttttaaacca atacctccct
     7141 gtcctttcaa gcgtggatag tgaattttca ttcgcaattc tgctgggaaa gaaagatcct
     7201 gagcaaattg aaatagatca caatttctca tattttcagg tgtttcccca ataggcaaca
     7261 atgctaaata actactccct aactcatctt ctaattgaag aaggccttta tgttccttcg
     7321 gtatacgtac atacgttcct tgattgtctt tcagtacttc ccaattattt tgttcttcta
     7381 ctttgtgagc cattcctcga atatattgca gacgattgag atacaacagc tcttttttta
     7441 ctaagggacg aatagaaaag ctataaagta attgtgtcac ttcttttaca taaggatcac
     7501 tttcagaaaa caaagtttcc aaatcatcta cttctaatcc aaataagcgt gctaaacttc
     7561 ctgctaaatc taaccagatt agatttatta catcatgaaa actctccacc gttgcttttt
     7621 ctttgacttt gattcctaaa ataaatgaag gacgatagac ttgaccttct cgttccaaca
     7681 atgtcactgt ttcttttgca taatacgcac caattgcttg actatcggga gaaaatccct
     7741 ttcctaatgt gtcaaacttc gtccgtaaat tcattgttcg tggatacatc accaactcca
     7801 catcttcata atctgctaaa agcaacagta aacgagccat catttctttt atttcctgtg
     7861 tcttttttgg tgtgtttttt gaaattgttc gtggaaaaac atgatagtag gcccagatac
     7921 aatcctctcc atttaacaaa caatttttct tgaatgcttt tactggttct tttacaagca
     7981 ccaatgaatt tcacctccca ataaaaaagg aacaaccagt tgattgtccc tttttctaat
     8041 cctctaaata aacatactta ttcacaaaaa gtaattcttc caatgcattg tttgatacct
     8101 tgtcccagtt ataatacaca caatcaccat tcatcatacg aatcatataa caaaa
//
LOCUS       DAEGIQ010000111         8151 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000111 DAEGIQ010000000
VERSION     DAEGIQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.113"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            476..1339
                     /locus_tag="KMT45_002340"
     CDS             476..1339
                     /locus_tag="KMT45_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBE7005785.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="KMT45_002341"
     CDS             complement(1468..3090)
                     /locus_tag="KMT45_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7005786.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="KMT45_002342"
     CDS             3339..3860
                     /locus_tag="KMT45_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBE7005787.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="KMT45_002343"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="KMT45_002343"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBE7005788.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="KMT45_002344"
     CDS             5941..6759
                     /locus_tag="KMT45_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBE7005789.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="KMT45_002345"
     CDS             complement(6862..7059)
                     /locus_tag="KMT45_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005790.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="KMT45_002346"
     CDS             7228..7806
                     /locus_tag="KMT45_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBE7005791.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="KMT45_002347"
     CDS             7901..8038
                     /locus_tag="KMT45_002347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005792.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2436 a         1474 c         1608 g         2633 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcgaagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg t
//
LOCUS       DAEGIQ010000112         7960 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000112 DAEGIQ010000000
VERSION     DAEGIQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.115"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            84..860
                     /locus_tag="KMT45_002348"
     CDS             84..860
                     /locus_tag="KMT45_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005793.1"
                     /translation="MMKKNYLKTGIFLVIGTILVIQLSGCRNKDKDFYGYWSGTAEKI
                     PVDYNFKITKDRVIELQKNGEMVDFKYTLRHKKFDSTTFRSIMISKDELVKAIPELKE
                     TTGISNITFMPIDGEKDKLTVLVNLDEHLSTATLYRTTKDESPIGKVEKKNDTISRQT
                     VDKKIDTFIKTYAAIPPTWKLVNEDKGITINTDTEIPTDAQSLSDLLANLTGVSNAVS
                     EQVGKDIPLRLLENGKKDYSVVFKNGKVTDYNEIFEKVIE"
     gene            2634..3401
                     /locus_tag="KMT45_002349"
     CDS             2634..3401
                     /locus_tag="KMT45_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HBE7005794.1"
                     /translation="MVEEEQGRLIKSKKRVQKHGEVFTPQRTVKDMLDLPGIKEACEE
                     LQTTFLEPSAGEGAFLVEVLTRKMAMVAREYNESLEQYENYSLLALSTLYGVELLEDN
                     AQTCVMNMFQVFNDLYREQSKKHGTAMKRKVQDSAKTLISRNIKQGDFLTKRTPDDNP
                     IIFSEWTPKTLRKGQKKITVNRTEYSLEDIYSGVTREFGEAYSALPVFEQLDLFSFDD
                     EVDEEVSNDPILRYVAMPITEVYKEEMEEIDDDSVEI"
     gene            3379..7776
                     /locus_tag="KMT45_002350"
     CDS             3379..7776
                     /locus_tag="KMT45_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019788126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HBE7005795.1"
                     /translation="MTTALKSKAKYFDYLPKYGSKLEHFLGIPNLVDAINTYRVTVNQ
                     IPNEVAIGTRRYSEVAARKIASNVGIDQQSMKYVDVLRELRVRNNITPTIADLFHKLR
                     KYGNVAAHENGIIPVSEAVESLVTLDTLLRVFARNLSGDKSLNPKEEITDEMFVITYN
                     TFDRKLIYIQSAKDADGTWKMYQGLEKIGDASVPENYEADFTPNSEYLREYASRRIKQ
                     YMTTAGVPFVLHWAQLAVNKKNQFFRDHDVHEVLKRSGYNPQTIGEDDTGKPNEWFSV
                     TVDVAKKAIQAVKDGKGSLDSEKDDFDEFKINFRPEQKDAIEQTKTVFKTKDEMLWNA
                     KMRFGKTLSSLQVIKESQYKKVLIMTHRPVVSDGWFTDFKKIFTDGSYIYGSKQDGEK
                     IQNLVDTEKPFIYFASIQDLRGSEWAGGKAGDKNYEFLAIDWDFIIIDEAHEGNETEL
                     ANNVKAKLRKEHTKVLELSGTPFNLFDKYDEDNIFTWDYTMEQEAKEKWSVEHPNEPN
                     PYEGLPKVSMFTFEIPNKFDYFDENKAFNFREFFRVKEDDETKLLHEGDVRKFLDYIT
                     TNDEKTNFPFSKPEFRENLRHTLWLMPGVKEANAFERVLETHPIFKDYNIANVVKTGD
                     SEHASDSDLELVRKAIGNPAKSKTITLTVRKLTTGVNVPEWTGVFFLSNTESPTSYLQ
                     AAFRAQTPFNHAELGIKKNCYIFDFAPDRALKIMSGSVGLTSKKGKVNSSEQKKKLER
                     MLNFLPILGKQGNVMKEFSVDRMLTALKKAYAEKAVRTGFEDSSLYNDNLLNLDQADL
                     TKFEDLKKIVGSSKPTKVKDFIVSDNGMSEEEYEKASRAEKKPKRQRTKEEQEAIDRA
                     KRLREQRNKMISILRGVSIRIPMMIYGMDIDIKDDVTIEMFLNLVDEASWNEFMPEGL
                     SKGRFREFIEYYDADVFVEAGRIIRRRAKSYDELDVIERTRKIGELFSTFKNPDKETV
                     LTPWRVVNMHLGNTIGGLSFFDEEYQNSTENGQPIVRWRKTDCTDDIFKENRKILEIN
                     SKTGLYPLYAATSMYYVQLLKQSDINAGRVDSEAIWEKVLSEQIFVISKTPMAKTITK
                     RTLIGYNAWKVNVEYSSELVTSMKRSINDGLTNLKGVYKNVKFDVIIGNPPYQEKDGG
                     SGASATPLYNLFVETSVKLSERYVSMITPSKWFAGGKGLDKYRDFMLNSSSLKVMVDI
                     VNGKEIFPNTSIGGGVSYFLWDKKYNGLTQYTNQHDGVSNTQERKMNEFPVFVRYNEA
                     ISAIHKIGEHASVGDFVISRNPFGLPTKIRGEEVQDSRNSIKVISSGGVGFVPLEDIT
                     KGQDMVGKYKVISSSLTSEHAGEPNKNGMFKVISTNRVIKPNEVVTDSYLVLFSADTE
                     TEAINYLKYLRTRFYRFLLLQAVTSIHLSKDKFMFIPVQDFTSGSDINWNVGIEEVDM
                     QLFKKYSFDASEILFIDQTIVPILHEEYY"
BASE COUNT         2756 a         1202 c         1718 g         2284 t
ORIGIN      
        1 aataattgat ggaaattgtt tttaaagagc tttaaaaatt gggttcttac attgttgatt
       61 aaatgcttta aaagaggagc tttatgatga aaaagaatta tttaaaaaca ggtatatttt
      121 tggttatagg aactatactg gttattcaat tatcaggttg tagaaataaa gataaagatt
      181 tctatggata ttggtcagga actgctgaga aaattcctgt agactataat tttaaaataa
      241 ctaaagacag agtaatagaa ctacaaaaaa atggtgaaat ggtggatttt aagtatactc
      301 taagacataa aaaatttgat tcaactacat ttcgttcaat catgatatca aaagatgaat
      361 tagttaaagc gattccagaa ttaaaggaaa ccacgggaat aagcaatata acgttcatgc
      421 ctattgacgg tgaaaaagat aaattaactg tgttggtaaa tttggatgaa cacttatcaa
      481 cagccactct ttataggact acgaaagatg agagtccaat tggtaaggta gaaaagaaaa
      541 acgatacaat atcaagacaa actgtagata aaaaaattga tacgttcata aaaacatacg
      601 cggctatacc acctacttgg aaacttgtaa atgaagataa gggaataacg ataaatactg
      661 atacagagat tccgactgat gctcaatctt tatcagattt attagctaat ctaacaggag
      721 tcagtaatgc ggtgtcagag caagtcggta aagatattcc acttaggtta cttgaaaatg
      781 gaaaaaaaga ttattctgtg gtatttaaga acggaaaggt aactgattac aacgaaatat
      841 ttgagaaagt cattgaataa tggtttttct atctttttat taactaccct accccgttgt
      901 tttttgaccg tgtttcgaga ggaatcatta gaaagcatag gctaactagg ggtttctttt
      961 tcttttattt tttgggaatc cgcttaagtg aaatgtatct gaaatgaaac ggagataaag
     1021 tgattttttg ctgtaatgac aaggttttag ctgtttcaga taccatgtaa cccaaaatag
     1081 tttattgaga aaataggaga gtatttgata ctaactagta gggaaaaatg agataaaaag
     1141 taaatggaat catttcaggt aatctgaaat aaaaaaatat acagtcctgt ggcactttac
     1201 ctatgggact gtatattttt tactctcagc gttttttgac tccgtgtctg actccgtttt
     1261 attgatttta tatgttattc agtgaccctt taacaggtca atttttatgt ttttgtcttc
     1321 gtatgactca gtttgatacc tagtgatttc catggtaata cgggatgttg aacaagctag
     1381 agaagttctg caggggttaa ttgcaaacaa ttcagataaa caaattacaa tagattattt
     1441 caagagggaa attaaaaaat cagaggaaaa atatttagat ttaaagtcgg atatcctatt
     1501 agcagacgat ataggttcac taactgtcga agaagatagc ctattctacg tctattgtgc
     1561 attgacttcg taaaagaaga cgtcttttcc aggtatacag ttcttttatc tacttcatat
     1621 gaaaatttgg gtacaaacga caaggaagtc agttttctgg tcagactatc gtacgaacat
     1681 cttgttgtgg tatttacact cagctttgat gatgagtaca agagtttttc agcgaacaag
     1741 acagaacatc gaaagaggtt tccagacgaa gttttacagg ctgggctgaa gtattatcaa
     1801 gaaggactca ccattaagaa gtctttatcg agaatataag aagatacagg tatagaagtc
     1861 agtcggtcaa cattctgtga ttacctaaac agcaaggagt aacaggtgaa cgggaaaaag
     1921 agcacaggga gggctatgtt cactggctga agaagcaaga taaacctgtt tatgacttgt
     1981 tctttgaaat aaagaaagct ttagatagtc agtcattaga gaacttcatt gatgggtatg
     2041 atgaaaagtt atctaatgag caaatgattg cgataatgaa agaggtgttt cgtcagtatg
     2101 ggttatcaaa taaacgtagg aaagaggttt ttgaccacgt taagacaata atgagctttt
     2161 accacacgac cctcggctaa atctaaagag ggcgaaaaag ggacgagtgg taaaatttaa
     2221 tgggaatagc taaaagtaaa taataataag tcaaagactg cctagtttgg tggtcttttt
     2281 tcatgttcat aaaaataaaa taggacagac gaacatcttc gctaactccc ctgtgttgca
     2341 agaggcaaac tctataattc tccagttaag ctatattttg ttctttattt tgagatagag
     2401 gaaaaaacaa aaatgtgtta aaattgaaca taagcataat tagaaattgt cgagtttttt
     2461 caagtaaaaa tcttgacttg taaggaggaa aagggcatga tgacacgcaa tcacagtact
     2521 agccagcagt ctgcccaaaa gcccatatat gaaagtgata accgattcgt agcatccgtt
     2581 tcggttattt gtgttgggaa gatgggtgat ttttagtggt tgaagatatg gtgatggttg
     2641 aagaggaaca aggacgacta atcaaaagta aaaaacgtgt ccagaagcac ggtgaggtat
     2701 ttacaccgca aagaactgtc aaagatatgt tggatttgcc aggcataaaa gaggcttgcg
     2761 aggagctgca gacgactttc ttagagcctt cagctggaga aggtgctttc ttagttgagg
     2821 ttttgactag aaaaatggca atggttgctc gtgaatataa tgagtcccta gaacagtatg
     2881 aaaattactc cttactggcg ctttcaactc tatatggcgt ggagttactc gaagacaatg
     2941 ctcagacctg tgtaatgaac atgtttcaag tattcaacga tttgtacaga gaacaatcga
     3001 aaaaacatgg taccgcaatg aaaaggaaag tccaagacag tgcaaagact ttgatttcta
     3061 ggaatatcaa acagggagat tttcttacaa aacgaacacc agatgataat ccgattattt
     3121 ttagtgagtg gacacctaag acgctaagaa aaggacagaa aaaaatcact gtaaatcgaa
     3181 ctgaatattc tttggaggat atttattcag gggtaacacg agagtttgga gaggcatact
     3241 cagctctgcc tgtatttgaa cagctcgatt tgttttcatt tgatgacgag gtcgatgaag
     3301 aagtttcaaa tgatccaata ttacgttatg tagcgatgcc gataactgag gtttataagg
     3361 aagagatgga ggaaattgat gacgacagcg ttgaaatcta aagctaaata cttcgattac
     3421 ttacctaaat atggttctaa gctagaacac ttccttggga ttcctaattt agtagatgca
     3481 attaacacgt accgtgtaac cgttaatcaa atacctaatg aagttgctat tggaactaga
     3541 cgttattcgg aggtagcagc acggaagatt gcttcaaatg tgggtattga ccagcaaagt
     3601 atgaagtatg ttgatgtact tcgcgagctt agggtcagaa ataatattac tcctacgata
     3661 gctgacttat ttcataagtt gagaaaatat ggaaatgttg cagcacatga aaatggaatt
     3721 atacctgttt cagaggcggt cgaatcttta gttactcttg ataccttgct aagagtcttt
     3781 gcacgcaatc tgtctgggga taaatcgtta aacccaaagg aagaaattac ggatgagatg
     3841 tttgttatca cctataacac ctttgaccgt aaacttattt acatccagag tgccaaggat
     3901 gctgacggca catggaaaat gtaccaagga ctggaaaaga ttggggatgc atcagtacca
     3961 gagaattatg aggcagactt tactcctaat agcgagtatt taagagagta tgcaagtaga
     4021 agaataaagc agtacatgac aacagcgggg gtaccttttg tccttcattg ggcgcaattg
     4081 gctgttaata agaagaacca atttttccgt gaccatgacg tgcatgaagt attaaagcgt
     4141 tcgggctata atcctcaaac gattggcgaa gatgacacag ggaagcctaa tgagtggttc
     4201 agtgtaacag ttgacgttgc caagaaagca atccaagcgg ttaaagatgg taaaggtagt
     4261 cttgactcag aaaaagatga ctttgacgag ttcaaaatta attttagacc agaacaaaaa
     4321 gatgctattg agcagactaa gactgtattc aaaacgaaag atgagatgct ttggaatgcc
     4381 aagatgcgtt ttggtaagac gctttcttct cttcaagtga ttaaagagag ccaatacaaa
     4441 aaagttctta tcatgactca tcgacctgtt gtaagtgatg gttggtttac ggatttcaaa
     4501 aaaatcttca cagatggttc gtacatctat ggttcaaaac aagatggaga aaagattcaa
     4561 aaccttgttg ataccgaaaa acctttcatc tattttgcaa gtattcagga cttacgcggt
     4621 tctgagtggg caggtggaaa agcaggggat aaaaactatg aattcttagc gattgactgg
     4681 gattttatta ttattgatga agctcacgaa ggaaacgaaa cggaattagc taataacgta
     4741 aaggctaagt tgcggaaaga gcataccaag gtcttagagc tttcgggtac accatttaac
     4801 cttttcgata agtatgatga ggataatatt ttcacttggg attatactat ggagcaagaa
     4861 gctaaggaga aatggtcagt agagcatccc aatgaaccta acccatacga aggtttaccg
     4921 aaagtatcta tgttcacctt tgaaatacca aataaatttg attatttcga tgaaaataag
     4981 gcatttaact tccgagaatt ctttagggtt aaggaagatg atgaaacaaa gttgcttcat
     5041 gaaggtgacg tacgcaagtt tcttgattat attactacaa acgatgaaaa gactaatttt
     5101 cctttctcaa aaccagagtt tagagaaaac ttacggcata ctttatggct gatgccgggg
     5161 gttaaagagg caaatgcttt tgaaagagtg ctcgaaacgc accctatatt caaagattac
     5221 aatattgcaa acgtagtcaa gacaggtgac agtgagcatg ctagtgattc agatttagaa
     5281 ttggtgcgta aagctattgg taatccagct aagtctaaga cgataacctt aacagttcgc
     5341 aagcttacaa caggagttaa tgttccagag tggacagggg tatttttcct ttcaaacaca
     5401 gaaagtccta catcttatct gcaggcagcg ttccgtgcac agacaccttt taaccatgca
     5461 gagcttggaa ttaagaaaaa ctgctacatc tttgattttg cacctgaccg tgctttaaaa
     5521 attatgagtg gaagtgtcgg actaactagc aagaagggaa aggttaatag ttcagagcaa
     5581 aagaaaaaac tagagcgcat gctaaatttc ttacctatcc ttggtaaaca ggggaatgtg
     5641 atgaaagaat ttagcgttga ccgaatgctt acggcactga agaaggctta tgcagaaaaa
     5701 gctgtacgga ctggctttga agacagctct ctttacaatg acaacctatt aaatttagac
     5761 caagctgatc ttaccaaatt tgaagacctt aaaaaaattg ttggctcaag taaaccaacc
     5821 aaagtgaaag acttcattgt ttcagataac ggtatgagtg aagaggagta tgaaaaagct
     5881 tcgagggcag aaaagaaacc aaaacgtcaa cgtaccaaag aggaacagga agcaatagat
     5941 agggcaaaga ggcttcgaga gcagcgaaat aaaatgattt ccattttgag gggcgtttct
     6001 attcgcatac ctatgatgat ttatgggatg gacattgata ttaaagatga cgttactatt
     6061 gagatgtttc ttaatttggt tgatgaggct tcatggaatg agttcatgcc agaggggtta
     6121 agtaaagggc gattcagaga gtttattgag tactatgatg cagacgtttt tgtggaggca
     6181 ggacgcataa tccgtagacg tgcaaagtct tatgatgagt tagatgttat tgaacgtact
     6241 aggaaaatag gtgaattatt tagcactttt aaaaatccag acaaggaaac cgtattgact
     6301 ccatggcgag ttgttaatat gcaccttggt aacactatcg gtggactttc cttctttgat
     6361 gaagaatacc aaaattctac ggagaacggt caaccaatcg tgcgatggag gaaaactgac
     6421 tgtacagatg atatttttaa ggaaaataga aagattcttg agataaactc aaaaacaggg
     6481 ctatatcctt tatatgcagc aacgagtatg tactatgttc agctccttaa acaaagcgat
     6541 attaatgctg gaagggtaga ttcagaggca atatgggaaa aagtattatc agagcaaatt
     6601 tttgtcattt ctaaaactcc aatggctaag acgataacga agaggacttt gattggttat
     6661 aacgcatgga aggtaaatgt tgaatactca agtgaattag ttacgagtat gaaacgttca
     6721 atcaatgacg gtttaacgaa tttgaaagga gtttataaaa acgtgaaatt tgatgtgata
     6781 attggtaacc caccttatca ggagaaagat ggaggttcag gagctagtgc tacaccgcta
     6841 tataatctat ttgtagaaac ctctgttaag ttatcagaac gctatgtttc gatgattact
     6901 ccttcgaagt ggtttgcagg tggtaaaggg ttagataagt accgtgactt tatgcttaac
     6961 agctctagct tgaaggtgat ggtggatata gtaaatggga aagaaatatt cccaaacacc
     7021 agtattggtg gcggtgttag ctatttcttg tgggataaga aatataatgg attgacacag
     7081 tatactaatc aacatgatgg tgtatcaaat acacaagagc gaaaaatgaa cgagtttcca
     7141 gtgtttgtgc gatataatga agccatttct gcaattcaca aaattgggga acacgctagt
     7201 gttggtgact ttgttatatc acgtaaccca tttgggcttc caacgaaaat tcgaggggaa
     7261 gaagtgcagg attccagaaa ttcaataaaa gtaatctcta gtggtggggt aggctttgtt
     7321 ccactggaag atatcactaa aggacaggat atggttggaa agtataaagt cattagtagc
     7381 tcactgacta gcgaacatgc aggtgaaccg aacaaaaatg gtatgtttaa ggtgatttct
     7441 actaacagag ttattaaacc taatgaagta gttacagatt catacctagt gctattttct
     7501 gctgataccg aaaccgaagc aattaattat ctgaagtatt tacgaacacg tttttacaga
     7561 tttttattac ttcaagcagt gacatctatt cacctatcca aagataagtt catgttcata
     7621 ccagtacaag attttacctc aggttcagac attaactgga atgtaggtat tgaagaagtt
     7681 gatatgcagt tgttcaagaa atattctttc gatgcaagtg agatattgtt tattgaccaa
     7741 acaatcgtcc cgattcttca cgaggaatat tactaagaga acaagctaac aaatgaggct
     7801 tagaaccctg ttttcttgat taattgcccc actttaaatc aagatttaac tgtctattat
     7861 caacaggtca aggtaaaata aaggatgtta gcaagtaaag agattgattt gagtctacat
     7921 cgaacaaggg aataatccaa taaaaaatga tttagaatat
//
LOCUS       DAEGIQ010000113         7882 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000113 DAEGIQ010000000
VERSION     DAEGIQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.116"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(326..1075)
                     /locus_tag="KMT45_002351"
     CDS             complement(326..1075)
                     /locus_tag="KMT45_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE7005796.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1260..1957
                     /locus_tag="KMT45_002352"
                     /pseudo
     CDS             1260..1957
                     /locus_tag="KMT45_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
     gene            1997..2554
                     /locus_tag="KMT45_002353"
     CDS             1997..2554
                     /locus_tag="KMT45_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBE7005797.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2562..3701
                     /locus_tag="KMT45_002354"
     CDS             2562..3701
                     /locus_tag="KMT45_002354"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBE7005798.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFYMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            3713..4144
                     /locus_tag="KMT45_002355"
     CDS             3713..4144
                     /locus_tag="KMT45_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE7005799.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4158..5603
                     /locus_tag="KMT45_002356"
     CDS             4158..5603
                     /locus_tag="KMT45_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBE7005800.1"
                     /translation="MEVANKKKRVSIRAKVQAIGGFLTNMVLPNIGVFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDNFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            5889..7514
                     /locus_tag="KMT45_002357"
     CDS             5889..7514
                     /locus_tag="KMT45_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBE7005801.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPANRLTAEQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
BASE COUNT         2642 a         1152 c         1592 g         2496 t
ORIGIN      
        1 atttgtatta tacgaatcaa attcctcaat cgtcagtgaa cttgctaatt taatgatagc
       61 attgctgttt actcccgcgt tgagagtcat aactattttg gtctgtttag tgctattgtt
      121 ttttgttaat aaaaagaaat aacgtagtat ttcaaaagac aaagaagtgg gatgatgatt
      181 gctatagtat gttatacgtg gtgctaggta agtgagaata aacaaaaaag gaaacacgca
      241 ataaagttta tttcaaaact ttattgcgtg tttctttttt agatttttta tatctgttgc
      301 tttagtagtt accaatagtt ttcgcttaat caatcaaaac gattttttgt ttattgtatt
      361 ctttacgaac cttttcagga atttcttctg aaataagata ggtaaggtct tcaattggac
      421 cgacgatatg attactgatg gtgccgaatt tatcattagt tatcatccca agtacttcag
      481 tagaaatacg tatcatctca cgtttaatgt cggattcttg catagttggc acgctcgttc
      541 caacatctag atctatgtta taaataccca taatataaag gtcggctcga atgttttgta
      601 gttgcttata agcttgtacc ccaatattga tcatagattg tttataaaaa gtaccgccaa
      661 tattgataat ttccaaatta ggatgctgtg ctaatgctat ggcaatcggc gggctattgg
      721 taatgattgt tgcacgaaaa tcttgtggga tttcttttgc taaagccaag ttagttgtac
      781 ttccatcaat gatgatgaca gattcttctt ttaaaaaagg gagggcttta tgagctaatg
      841 cagttttttg ttcgctagct acagtttctc ggtagttaaa attggtaatt ggcggaccaa
      901 cacgtaaagc gccgctgtgt actcttttta agagaccttg gctatccaaa tctttcaaat
      961 ctcgtcgaat cgtgtcttcg gatacattga aacgttgact taaatcacta gcgataactt
     1021 tttgttctgc gtttaataaa tctagaattt tttgatgacg ttctaatttc aacatgttgc
     1081 gtgctccttc ttatttattc ttgtttatca tgtcatttat gcgtttttat gtcaataatt
     1141 tccgttttaa tacactaaaa cggcataaac aagcaaaaac aaaatatttt tatgcagttt
     1201 tatgttgact agtatttgtt aagcggttac aatgaggggg tagttaaagg agggaatgta
     1261 tggagaaaaa aattattttg tcgccttcac ttatgtgtgg ggatctagca aatttggaag
     1321 gaagcatcca agaactagaa agtattggga tggattcctt gcatattgac gtgattgacg
     1381 gttcttttag tcccagtatg cctttaggga tcgaaacaat caaaagaatt agagaaatca
     1441 cttcaatgaa ttttgatgta cacatcatgg ctaaggataa cgaatttttt attggagaaa
     1501 tgttgaaaat tggcgtagag agcattactt ttcattatga aacaagcctc catattgatc
     1561 gatatatcca gctcatcaag aattctggaa caaaagtagg gatagctttg aatccagcaa
     1621 ctccgttaaa tgtattacaa tatattttac ccgagattga taggatttgt ttgatgctga
     1681 ttaatccagg ttttgcaaca aacaagtcag aaaaacaaat ttcttacgcg ctaaaaaaat
     1741 tacagatctt tgccagttag taaatgagaa agagttagca gtagatatcc aagtggatgg
     1801 acgggtatcg ttagaaacga tcccgggatt attagatgca ggcgcaacaa atctggtatt
     1861 aggaagtaca agtttgtttc ttaaaggaaa tacattagca gaaaatcaaa aactacttta
     1921 tcaagcgatg aagcaatcag ttaggaaaaa tcaatagatg tagtggtcct gaataataga
     1981 aatggagggg aaatagttga gttataaggc ggcgaaacaa aatattgtaa gagaaattac
     2041 tgaaacatta gaccgtattt cttcaaaaga tgtggaacaa ttgataaaga aaattgaaaa
     2101 agcggaacga gtttttttcg tgggggttgg cagagtactg ttagcgctag aaggaatggc
     2161 aaaacgctta gggcatctag gaattcagac agtagtcgta ggacaaatca ctgaaccggc
     2221 aattacagat aaagatttat tgattgttgg ttctggtagt ggggaaagtg ctttcccact
     2281 aactattgca aaaaaagcaa aggaatttgg gtcccaagtt gcccatatag gatcaaaccc
     2341 agacagcaca atgaaagaat attcagatct ttttattcgt atacccgtac aaacaaaatt
     2401 agcgttacca gaggaaattt cttctataca gccaatgact agcctgtttg aacaaagtgt
     2461 tcttttgtta ggagatagta ttgctttagt gatgatagaa gaaaaagcaa ttgatatgtc
     2521 aaagttgtgg caataccatg ctaacttaga gtaggtgaaa aatgaaagcg gtacatttcg
     2581 gagcagggaa catcggcaga gggcttattg gacaaattct acatcagaat ggattcgatc
     2641 tatgttttat tgatacgaat cctgaactga tccatcaatt aaatcaagat aagggttata
     2701 ctatcgaaat tattgatgaa atgaacagga gtgatttcat tgatcatgtt gtggcactga
     2761 atagtttatc cgagctagaa gaggtgatta cagcaacaaa aaatgcagat atcttaacaa
     2821 catctgttgg cgcaataaat ttagtaaaaa ttgcaccgat cattgcgcaa gccttaagta
     2881 agcgatttga acaaaagcat agcatcaata tcgtagctaa tgaaaatgtg ataaatgcaa
     2941 gtgatgtgtt gaaaaaagaa atttattctt tgctgtcaga aaaagaacaa gagaattttg
     3001 atgaatatgc atattttgtg aatacggcaa tcgatcgaca atctttagga aaagttcttg
     3061 gaggaaaaat gatctctgtt gtcgaacctt attatgaatg gataatggat cgacagcagt
     3121 tagatccaac tacatctttt tatatgaaaa atgtaaattt ggttgatgat atggagcctt
     3181 acattgagcg aaagctttat atcgttaatg ctgctcatgc ggcagtagct tatttaggcg
     3241 cactgaaagg atataagacg attcaagaag caatccaaaa tatggagatt ttcaatctag
     3301 tcaagcaatt tttaatggag aatatagagt actttgtaca aaaatatcat ttttctagag
     3361 aagaattaac atcttttgtg gaaaaaacat taaaccgtca tgggaatcag aaactctccg
     3421 atgaaattac tcgtgtagga agttccccta ttcgaaaatt aggccctaat gatcgtttaa
     3481 ttgctcccat agtaaaacta gattcatatc atttacctta cgatgcagga ataaaagtaa
     3541 ttgctgctgg atatcattat cggaatcttg ctgatccaga agctgaacaa ctacgtcgaa
     3601 tgattgtaaa tgatgggttg aaagcaacga ttaaaaaaat ttctcgtttg gaaggtaagc
     3661 tgctgaatga agttgtcgct gcttatgagt cccagattta gaggaggaga atatgctaaa
     3721 agaacaattg tcaggaaata caatgtttat ggatgcgatc gattcatggc aagaggcaat
     3781 ccgagtaagt gcgcgtcctt tgataggaaa gggaataata gaggaaagct atgtacaagc
     3841 gatgatacaa aatgtgttgg ataacggaaa ttatattata ttactgccac tagtagcaat
     3901 gcctcatgcg cgtccagaat ttgggagcaa aggggtcggg atgagctttt tatgtttaga
     3961 taagccagtc atgttcccaa gtgaagaacc agtaaaattc ttctttacct tttcttctga
     4021 ttcgccaaag ggacatttag acttaatggc caatcttgga gaattgctga gtgatgaaaa
     4081 aatgtatcaa aagttattcg atgtgaagac agaaaaagca ttgttagatt tgataaagaa
     4141 gtaaaggaga gaagaaaatg gaggtagcaa acaaaaaaaa acgagtctcc atacgagcta
     4201 aagttcaagc gattggcggt ttcttaacaa atatggtatt gccaaatata ggcgtattta
     4261 ttgcatgggg aattgttact gcattattca ttccaaccgg gtggttccca aatgaatctc
     4321 tgaatgaact cgtaagtccg acagtcagct atctattgcc cttactactt gcctatactg
     4381 gtggtcggat ggttggtggt acacgtggag ctgttttagg tacaatcgga acaataggtt
     4441 tgattattgg tgctgacata ccaatgttca tgggagcaat gattatgggg ccacttggtg
     4501 ggtggatgat gaaaaaaaca gataattttt tagatgggaa agttcctgct ggttttgaaa
     4561 tggtcgtcaa taatttttct ataggaatta ttggtttcat actgatgtgc ctttcttatc
     4621 tggtagttgg accaataatc caatcagcta atcaatttgt aacgagtgca attgaagcat
     4681 tagtttcaac tggtttttta ccgatactgt ccttaatcaa cgaaccggca aaagtcttat
     4741 ttttaaataa tgttattgac caaggtgttt attatccatt aggtatgcaa caaacattag
     4801 aggcaggcag atcaatttat ttcatggtag catcaaatcc tggccctggc ttaggactat
     4861 tgttagctta ttctttattt ggtaaaaagg cagcaaaaca gacagcacca ggtgcaatca
     4921 tcatccattt tttcggcgga atacatgaga tttattttcc atatgtatta atgaaaccac
     4981 tcactatatt atctatgatt gctggtggaa tgacaggaat catcactttt gaattatttg
     5041 atgcgggcct ggtagcaggc ccaagtccag gttctatttt ttcttattta gctctaacgc
     5101 ctcgaggaaa ttttttgggt gtcatggcgg gagtggtcac agcaacactg gtttcctttt
     5161 ttgtaacatc agtaatttta aaagcagata aatcaacaga tgaagacaga aattttgaac
     5221 tatcagttca aaaatcgaaa acgatgaaat cagaaggaag aaaattatta aaagaagaaa
     5281 atgctagaga aatatgtacc gagaaaaaag agatgaataa aatttccttt gcttgtgatg
     5341 caggaatggg aagcagcgca atgggcgcaa caatattcaa gaaaaaattg caaaaagcag
     5401 gtattactga tgttgagata aaaaattatc gaattgaaga ggtaccagaa gatagtgatt
     5461 tggtgatcgt acatcaagat ttggaggaac ggactagaaa agcacatcct aacatgaaaa
     5521 ttgtgacatt aaagaattat ttgcaagatc cagtaatcaa tggattagca gaagaaatca
     5581 ttggattaaa aacagaagac taatttatgg gaatttttga ttaaccttta ttaaaattat
     5641 tttgcctaat ataaaaaggt tgtgacatgg cttgtgtcgc aacctttttt tgtcgcacaa
     5701 ataattaatc ggatatttta tgttcatatt ttttgtgatc aacgagaact gatggggcaa
     5761 agatacattt tagatacttt taactgttaa tttacaaaac aaggtgaaac gaatagaaaa
     5821 atgaaaaaat cgatacggaa gaatatgttt gattaaaaca tgcatgaaga gaaggaggtt
     5881 gatgatacat gaaaaaaata tattcaaagg aaatcgattg ttttagaggc attgctgttt
     5941 tagcagtgat attttatcat ttgccaaata gtttgctgcc aggcggattg caaggagtaa
     6001 cgatcttttt tgtagtttct ggttttttaa tggctaaaaa tacgataaga gatgaaaaaa
     6061 acagacaatt cagtttttta catttttact ataagcgtat caaacgtatt tatccatttt
     6121 tactgttttc tttggtcgta ttcattttca tactagggat tagcaacatt cgattgatag
     6181 gaaacattcg tagtgagcta cccagtttga tttttggcta taacaattgg tggcagctga
     6241 atcaaggagt atcttatttt gactcttact ttaatgcttc tttatttaaa cattattggt
     6301 cgctttcagt tgaactacaa ttctatttga tatggccatt tttgtttatt gtcattaagc
     6361 gtatgagaag aaaacaagtg ttttacttaa tctatacatt gatttttgtt tctattcttt
     6421 ttagtttgtt tttaccttca actaaggcat attatcatac gatagctaaa ctttttccat
     6481 ttttattagg agtttggggg tattttaatc gaataactat tggccgtttc tttgaacaaa
     6541 acacttttag taaaatctgg cttttattac tagctagtct ttgtctgatt ctgttcccga
     6601 tttttcctta tacactaaat gaactactga tcagtatttg ttttgctcta ttgttagcca
     6661 gtgtcgatga tatgaacatt gcaaaaaaga ccaaaatcgc tagtacaaaa ctttcttttg
     6721 taggaaaaat cagttatgaa ctttatttgg tgcatttccc agtattgatt gctctgacaa
     6781 atctattcaa acatcatcat tggaaatatt tggatatcgc aggtctattc actgtgtttt
     6841 tatgtacatt actcatgatt acttgcttta gaaaaggagt gcagaagatg agatcaccca
     6901 aaatttggac agctatggca ctaatgataa tcggagtttc tgtactggta ttacgtgctc
     6961 ctgcaaatcg actgaccgct gaacaaaatc agttgcaaag tttattaaaa gaaaactcta
     7021 gtaaagcaaa tgcaggagaa aatctgttgt cacaagagaa gaaaatatta tttatcggtg
     7081 attctgttat gctgggggcg tatcaggaac taaaagagac atttaaaaaa gaagcattgg
     7141 tagatgcaaa agaatcaaga caaatcactg cgttaccaga aatcttaaag gagcataaag
     7201 atttagtttc ttaccaacaa atagtgatcg ggttaggaac caatggtgtg ataacagatg
     7261 aagctataga acaaacaatg tctttgctga aagataaaaa agtatactgg gtaaatataa
     7321 aagctccaac tggctggcaa gatacgataa atgccacatt agctacattg ccaaatcagt
     7381 attccaatat aacgatgatc gattggtata gtgagagcaa aaatcatcct gaattttttt
     7441 atgatgatga aacccatttg aatgaaagtg gacgtacagc ttatgcaaag tatattgctt
     7501 cagttattca ataaggtagt gattacgtgt gttaacctta ctttttccta ataggtgaat
     7561 caatttaaat aaaaaatact tcatatgata aacggtgttt aagaggagga gctttttaat
     7621 attcgtggta aaaaagagat gggcgaggtc gctaactgag agcttcttgg aaagaaatcg
     7681 ctttggattt atcctctttg ttatacagaa catgagcgag acgacaatac taggatgtat
     7741 gttctgatcg aagttttatg gttacaagca gcaatcagct tagataaaat taaggatcgt
     7801 aggaaccaag taaagaaggc tgttagtaaa gaaacaaacg atcaagtatg ttttattctt
     7861 aagaagatgc agtgaataaa ta
//
LOCUS       DAEGIQ010000114         7853 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000114 DAEGIQ010000000
VERSION     DAEGIQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.117"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            24..221
                     /locus_tag="KMT45_002358"
     CDS             24..221
                     /locus_tag="KMT45_002358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005802.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(264..839)
                     /locus_tag="KMT45_002359"
     CDS             complement(264..839)
                     /locus_tag="KMT45_002359"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBE7005803.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(994..1520)
                     /locus_tag="KMT45_002360"
                     /pseudo
     CDS             complement(994..1520)
                     /locus_tag="KMT45_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1977..3488
                     /gene="gadC"
                     /locus_tag="KMT45_002361"
     CDS             1977..3488
                     /gene="gadC"
                     /locus_tag="KMT45_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HBE7005804.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3501..4901
                     /locus_tag="KMT45_002362"
     CDS             3501..4901
                     /locus_tag="KMT45_002362"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE7005805.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5066..6501)
                     /locus_tag="KMT45_002363"
                     /pseudo
     CDS             complement(5066..6501)
                     /locus_tag="KMT45_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6894..7487
                     /locus_tag="KMT45_002364"
     CDS             6894..7487
                     /locus_tag="KMT45_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBE7005806.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
     gene            complement(7471..>7853)
                     /locus_tag="KMT45_002365"
     CDS             complement(7471..>7853)
                     /locus_tag="KMT45_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE7005807.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
BASE COUNT         2452 a         1348 c         1439 g         2614 t
ORIGIN      
        1 ggctgtattt cttgatttgt ttattgaaag attcgattgt agaaccagaa ggaatggaga
       61 acacttttga actttacaac aaaactactg ttgataaatt aattccaatt ttagttccaa
      121 ctgatttaaa tggtcatgat gttttacttc gttttaacgg atttttaggt gaggattata
      181 gatacctaaa aatttctgat cctcaacctt taacagaata ataaaaaacg tgtaatcaat
      241 tatttgatta cacgtttttt atttcaatct tcgttgtagt agtggttgcg acataccgaa
      301 aggtaatctt cattacctcc tactacaatc tgttcacctg aataaactgg aataccatca
      361 acaatacgag cattcattac agccttttta ttgcaaaaat ggcaaatagt tttaatttcc
      421 tcaattttat cagcaaatgt caataatgct tcggatccct tgaagagatt attttgaaaa
      481 tcattcttca atccatagca aattactggt acttcctcat tcattacgat aagtgataga
      541 tcaataatat tctctctact taaaaattga gactcatcga ccaaaataca atcaactttt
      601 ccgtactgat caataatctc cactaattct ccgtacacat ccatttcaga agtaattgca
      661 atagctggtt tcgaaacacc tatacgactc tcaacttttc caaatcctga tcgagtatct
      721 attgcgcttg tcattatgat aactctcttc ccttgttctt cataattatg agccactttc
      781 agtagctcaa tactctttcc actattcatt gttccatatc tgtaaaataa ttgtgccatt
      841 aaatttattc ccttctttta caattcaatt attccttaat tccatctgct tttatgcagc
      901 tgctcgttta ttccttgata tgtctataac atctactatc cctaaaacca atttataagc
      961 cattagagat ggtattcctg aacatctctc atattgtttt ttgagtggta tatcatctga
     1021 aaaactgaag gaatcatgaa aatagcgaca tacttatcat ttagagttct aactttaaat
     1081 gcatttactt cttcagtcag cactttagtc atgttggaca tggtttgggg cgtgtagtga
     1141 tgcccataca ttttctcaat caagtctgcg atttccgaca tggtaatacc ttttcggaag
     1201 aggtgaatga cggtctcctc taatgtgtca ttcgtccgtc tataagcagg aacagtctgt
     1261 tgcttgaact cgccattgcg gtcgcgcgga atctggagat gaagttcccc atactcggtc
     1321 ttgaccgtac ggtcatagga gccgttacga gaattacccg tgttaaaacc aatgcgatcg
     1381 tacttttcgt aatcgaggaa agccgttaac tccgttttga gaagcgtatt gacagctttt
     1441 tccaaatgga cacggaataa tccattcaaa tcgcctttgt tcgctagagt ctttaggatt
     1501 tctgtagtaa aatcgttcat atggaagtcc tcttttctgt gaatgtgttg tggtaactct
     1561 attctacaga agggacttcc ttttttctat ttacacaaaa tattttacac tctcaacaaa
     1621 agtatcttcg attaatactt gctcaagtta tgtgcagcta cgaaaaagta ggtaccgagg
     1681 tactttcttt ttcgcagctt tttttggact tatgagaagt acattcgctg gaagaatacg
     1741 atataaaacg agctttttca ctaaagatat taatagagtg aaagaaacaa gcttttgttg
     1801 tcagtttttt cacttttcta aaaatgaaaa taaaaaattc tggaagtgga gaagaatttt
     1861 gcttgctata ctttgtcttg tagaaaagag gtagacaaaa taatgcaagc tcaaataagc
     1921 gattttccat aaccttggta ggaatgacaa attaaatagg aaagggaagt aataatatga
     1981 ataaaaaaaa gttatctcta ttcggttttt ttgccctgac agcttccatg gttttgactg
     2041 tttatgagta tccgaccttt gcaacatcac aattacattt ggttttcttt cttcttttgg
     2101 gaggtatttt atggtttcta ccagttgctt tatgtgctgc agaaatggca acagttgaag
     2161 gatggaaaga tggtgggatt ttcagttggg ttagtcaaac tttaggagaa cgttttggtt
     2221 ttgcagctat ttttttccaa tggtttcaaa ttacagtagg ttttattaca atgatctact
     2281 tcatactagg agcgctgtcg tatgtaatta attttccaga gcttaataca gatccattta
     2341 tcaagtttat tggtgtatta gtcattttct gggtactgac tttttcacag ttgggcggga
     2401 ccaatcgtac cgctaaaatt gctaaagcag gatttgtcat tggaattgtt attccttcga
     2461 tcattctctt tggtttagca gcagcctact ttatcggcgg taatccgatt caaatggcta
     2521 ttaatgcgaa agcttttatt cctgactttt ctcagctttc aactttagta gtatttgtat
     2581 cttttatatt ggcttatatg ggcgtagaag cttcagcatc acatattaat gagctggaaa
     2641 atccccaaag aaactaccca ttggcaatgt ttttactagt gattttggcc atcgccttag
     2701 atgcaatcgg cggtttctca gtagcagcgg ttattccaca acatgacttg tctctcagtg
     2761 ctggtgtaat ccaaacattt gaaacgctta ttttacattt taacagtcat ttacattggt
     2821 tggtaaaagt gattgctctc atgattgcct gtggcgttat gggtgaagtt agttcttggg
     2881 tagttggtcc atcacgaggg atgtttgcag ctgctcagcg aggtctgtta ccagttttct
     2941 tccggaaaac taataaacaa gacgttccag ttccgttgat tatgatacaa ggaattatcg
     3001 ttactatttg ggcagcagtc ttaacttttg gtggaggagg caacaattta tcattcttag
     3061 tagcaatctc cttaactgtt gtcatttatc tcgtaggata tttactattt ttcctaggtt
     3121 actttgtatt agtttttaag aaaaaagact taaaacgaac ttacaatgta ccaggaaaaa
     3181 ctgttggcaa aacgattatc ggtggaattg gttttatttt atcagtgttc gcattgctta
     3241 tctcttttgt accaccagct tcaattgcag ctagtgagca ccgaacatac caaatcatac
     3301 tacttattag ctttgtagtg actgttcttt taccttttat catttatgaa gtacatgata
     3361 aaagtgctca cacaactatt gaggaaccta ctcacgtgaa agctgaagac gttaatcccg
     3421 ctgtttatcc agctgctaga ggagagcacc acgttacaaa aaatgatgaa catattttaa
     3481 aacattagga ggaattcatt atgttatacg gaaaagataa tcaagaagaa aaaaactatt
     3541 tggaaccaat ttttggctct gcaagtgagg atgttgactt gcctaaatat aagttaaaca
     3601 aagaatccat tgaaccacga attgcttatc aattagtaca agacgagatg ttggatgaag
     3661 gaaatgcgcg attaaactta gctacttttt gtcaaacgta tatggaacct gaagcagtga
     3721 aattgatgac ccaaacgtta gaaaaaaatg caattgataa atcagaatac ccacgaacaa
     3781 cggaaattga aaaccgctgt gtaaatatga ttgctgattt atggcatgct ccaaataatg
     3841 aaaaattcat gggaacttca acgatcggct cttcagaagc ctgcatgctg ggtggtatgg
     3901 ccatgaaatt tgcttggcgt aaacgtgctg aaaaattagg tcttgatatt caagcaaaaa
     3961 aacctaacct ggtgatctct tctggttacc aagtttgttg ggaaaaattc tgtgtatatt
     4021 gggatgtgga actgagagaa gtcccaatgg atgaaaaaca tatgtcaatt aatctagata
     4081 ctgtcatgga ttatgtggat gagtacacaa ttggtattgt aggtattatg ggtattactt
     4141 acactggtcg ttatgatgat atcaagggtc tgaatgattt agttgaagct cacaataaac
     4201 aaactgacta taaagtatac attcatgttg acgctgcatc gggtggcttt tatgcaccat
     4261 ttactgaacc tgatctagtt tgggattttc aattgaaaaa tgttatctca attaattctt
     4321 caggtcacaa atatggtttg gtatatccag gtgtgggttg ggtcttatgg cgtgaccaac
     4381 aatacttacc agaagaatta gtatttaaag ttagttactt aggtggagaa atgccaacta
     4441 tggctatcaa cttctctcat agtgcagcac aactaattgg gcaatactac aactttgtac
     4501 gctatggctt tgatggttat cgtgatattc accaaagaac tcatgatgtt gctgtttatt
     4561 tagccaaaga gatcgaaaaa actggtattt ttgaaatcat taatgatgga tcagaattgc
     4621 cagttgtgtg ctataagctg aaagaagatc ccaatcgcga atggacacta tatgatttat
     4681 ctgatcgtct gttaatgaag ggatggcaag tcccagccta cccattacct aaagacttgg
     4741 atcaattaat tattcaacgc ttagttgttc gagcagactt tggaatgaac atggctggtg
     4801 attatgtaca agatatgaac caagcaattg aagagttgaa taaagctcat attgtttatc
     4861 ataaaaaaca ggatgtgaaa acgtacggct ttactcacta aaattcttca gagaaatata
     4921 aaagaagtta attaactaat aaaaggagtt gtgattacca cacagctcct taaatctatt
     4981 tgaataaata tatgatattc atttgttaca ctctcaatct tagctgttgt tctattcatc
     5041 attactctta ttgtaagtag ttcagtcaaa tttgcttgtt caattgatcg ataacagtat
     5101 gaattgtttg aaaagtaatc ggatatataa ttgttttctc actacctaag ccttcttggt
     5161 ataaagaact atcgcaaagt ataatccgct gagtacaaaa gaaagtgtcg tctactattt
     5221 ctctaagttg tttacaaatg atattactaa tagaaaagtg ttctcccaaa atgtcttcta
     5281 actgttgtcg atagtataaa tacctaaagt ctgacggagc tactatgaac acttcaattg
     5341 gagatttccg tttatgctca tttaaaagac ttaaactaat ggttagttta cgaatagaat
     5401 tctcattcca acgcagaaat aaattgtttt gttttcgcca ttgttcaaga atttcatgaa
     5461 cttctaagta caaagcgcgt tgctcttcat ttaacaagta agtcttatca ggttgaaata
     5521 tctgaatatc agcccaaagt gagcggataa acgggagaag tgctttatca aaaagtatct
     5581 gattttcttc agatatattc agagataaga taagtttttg actgagctca atcaccaaag
     5641 ggttatctgc tacatggttt tgataaacaa cgttcaagtc tttttgtaac aattcaaagt
     5701 tatcgcatag ataatttcta gagttgaata gaaagtagat ataaaggcat tcatcttcgt
     5761 catgacataa agagaaagaa agttgctcta atcctttctg tattagctga aacaagggag
     5821 tatgtcgggc aactcttttt tcttcctgac taaaatatat tgggttagtc ttagatctct
     5881 gcaaaccgat agcgatacct aataaaataa attgtttttc aactgtaggg taacttcttg
     5941 aaaaaaatgt ttttctacat aatcaatcaa ttgttgaccg ctttgaataa ttgatttatt
     6001 tagctggatc aactctgtcc gatttgtctc atagattatc gttagtaaca aacttcgata
     6061 atccttttca ggaatttcaa aatggccatc agcatttaag tactgcattt gtttaaaaaa
     6121 attaatcaac tcatttcgta agttgtaaac tttgcttaac gaaatgaagg tagtatcagc
     6181 tatattactc caattgtgtt cccctgataa ataatgtcct agtacattga aaaagtcaga
     6241 gcgctgatag atgagttgag tgagtgccaa cctcgaaaac gaagagtcga atgcgactgt
     6301 aaagctactt ctagatttct ccactgaaat aatttgctga tggagtatgt cggtaatgca
     6361 ttccaagtcg ttagtgacag tgagtgggca aacgttcaat tgatctgcta tttcgcgaat
     6421 ctgaatggtc cctttttcgt gtaattgctc acaaatgtaa acttgacgaa aaatactttt
     6481 ctccagatag gtttccaaca tagtactcct cctagtcatt ttatacataa ctattataac
     6541 tctaccaatc ggaatagtcc tgtaatatat tgaatcaaaa atataaatta taaaataatt
     6601 agatggggta tttggtttaa gtatccctta tatagtaata aatttatagg tgtgattctc
     6661 gaattaatac ccgaaaatgt gagagaattt ttatggattt tttaggatac aaattaatta
     6721 tttttgcttt tatcctgatt accagtccag tactgtcggt atcccatttt gcagcaaaat
     6781 catgttgata aagtgacaag aagactgatg agatatatta tccaaattga gaagataatt
     6841 aatcggattc ttgagtatac cctacttttt aagaaagtag ggtatacaag cgtatgaata
     6901 aagacataac tttccctgtg atggacgttc ggcttgttcc aattaccaaa atacaggcga
     6961 acgattataa tcccaatcga gtagcgtctc cggaaatgaa cttattggag ctttctatca
     7021 ttgaagatgg ctttactcaa ccattagttt gttattacaa ggaaaaagaa gagaagtata
     7081 tcttggttga tgtgcttcat aggtatcgtg ttgctaagga aagactcaac ttaaaagttg
     7141 taccggtaac agttattgat aaacccatag aagaacgtat ggcttcaaca attcgacaca
     7201 atcgtgcaag agggacccat gaaataagag gaatggaaat tgtcatccaa agattagtaa
     7261 aaaatggatg gtcagacaag agaatatcca aagaattagg catggacatt gaagaaattt
     7321 ttcgctttaa gcaagtgtct ggtggaatgg caacagtttt tttgacaaat tttataaggt
     7381 gcagaacttc tttccgtatg ctattccgat tgtccttgac aatgagcctc ctcggatgtg
     7441 acgatcttcg gcaggagcta acagttaaag ttagactgcc tcgctagcgt tagcacatcc
     7501 gagctcatta tcaaggattc gctgcgccaa tctctggtta cggtaaccaa ccggtgtctc
     7561 gtagccaagt gtaccgtgca accgaaggtg gttccaccaa tttacatagt caaataactc
     7621 caaatccaat tgttgtaagg tttcaaatgt gtattgatag acaaattcta ctttcaacga
     7681 cttataagtt gattcagcta cggcattatc aaaaggacag cctttatgac tcaatgatcg
     7741 attgatgtca aaagttgtta ataattcatc aatagcttgg ttatcaaact cttttccacg
     7801 atcagtatga aaaatctcaa cctctgtcag aggttgtttg atacggctaa atg
//
LOCUS       DAEGIQ010000115         7817 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000115 DAEGIQ010000000
VERSION     DAEGIQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.118"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            106..807
                     /locus_tag="KMT45_002366"
     CDS             106..807
                     /locus_tag="KMT45_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBE7005808.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1379..2395)
                     /gene="tsaD"
                     /locus_tag="KMT45_002367"
     CDS             complement(1379..2395)
                     /gene="tsaD"
                     /locus_tag="KMT45_002367"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBE7005809.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2392..2856)
                     /gene="rimI"
                     /locus_tag="KMT45_002368"
     CDS             complement(2392..2856)
                     /gene="rimI"
                     /locus_tag="KMT45_002368"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBE7005810.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2863..3405)
                     /gene="rimI"
                     /locus_tag="KMT45_002369"
     CDS             complement(2863..3405)
                     /gene="rimI"
                     /locus_tag="KMT45_002369"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBE7005811.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3389..4111)
                     /gene="tsaB"
                     /locus_tag="KMT45_002370"
     CDS             complement(3389..4111)
                     /gene="tsaB"
                     /locus_tag="KMT45_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBE7005812.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4302..5282)
                     /locus_tag="KMT45_002371"
     CDS             complement(4302..5282)
                     /locus_tag="KMT45_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7005813.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5306..6790)
                     /gene="galT"
                     /locus_tag="KMT45_002372"
     CDS             complement(5306..6790)
                     /gene="galT"
                     /locus_tag="KMT45_002372"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBE7005814.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6802..7791)
                     /gene="galE"
                     /locus_tag="KMT45_002373"
     CDS             complement(6802..7791)
                     /gene="galE"
                     /locus_tag="KMT45_002373"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBE7005815.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2183 a         1691 c         1384 g         2559 t
ORIGIN      
        1 tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa aactttacaa attctttaaa
       61 aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg tgttaataaa
      121 atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt ctcggatgaa
      181 gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg acagatcctt
      241 ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact cgttcgttta
      301 gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac aaataacctt
      361 tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca agcgatgact
      421 gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg aaaaaatccc
      481 gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt
      541 atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt attcattttg
      601 aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta tccgatcacc
      661 ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa
      721 caactacgtg attccggact gttagattat gacaaaaagc gttttacttt ctgtaagaca
      781 gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac cctgatattt
      841 atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc
      901 actgggcaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc
      961 aaaaaatact gatagattaa ctagaggaag cgtcttgtcc tcatccgacc tagattattc
     1021 gatattcctc gacaataaac gattaatcag tgagggcaat cagaggaacg gataatgatg
     1081 aataagctgt aaaaaatagg gctttgacaa aagcgtgtca cagcccttat ttcgattaaa
     1141 cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg
     1201 ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta
     1261 aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa
     1321 gccgtttttc ctgggctgtt cttattactt gatgcagggc agatttttca caacctcttt
     1381 attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt
     1441 gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag
     1501 ggggaatcac tagttctacc tcagggagtt tagttgccaa agcatcctgc aatccttcac
     1561 gcaacccttg attggccgct acacctccag ctacaatcag ttgcttgact ggataatttt
     1621 gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg
     1681 ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa
     1741 atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgtg
     1801 gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt
     1861 aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg
     1921 tctccccaat gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc
     1981 cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga
     2041 tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct
     2101 tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga
     2161 ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg
     2221 tgatttgttc tacgtgatgg cggctagcta cttcaggaac tacgccacca aaacgtttat
     2281 gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg
     2341 ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat
     2401 cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata
     2461 tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc
     2521 attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag
     2581 taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt
     2641 tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc
     2701 ttcaactaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt
     2761 cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa
     2821 gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag
     2881 ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt
     2941 tgcttgaaaa cctaacttcc gataaagtct ttgtgcatct tgattactca tacgcacttc
     3001 taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag
     3061 cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc
     3121 taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag
     3181 atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa
     3241 ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa
     3301 ttctcttagt acgtaacgac gatcgatttg ttcgattgtt ttttctgtat aaatcatttt
     3361 atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag
     3421 gtgtgtgcgt ctccaaccat ttttcttctg cttctacccg ttttaaatag cgaggaagaa
     3481 aagcttgttt ctctgcaact ggttcagcta aagcacctaa ttgagccaca gttacaccac
     3541 ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt
     3601 catagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta
     3661 gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacaatt tctaatctat
     3721 tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca
     3781 caataatttt atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta
     3841 ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc
     3901 ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt
     3961 gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga
     4021 tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt
     4081 ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct atgacattct
     4141 cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag
     4201 tacaatgata catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa
     4261 agctgcggta tcacttcatt tccccacagc ttttccatct tttatttcgt tgattctcga
     4321 ataatcaact ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat
     4381 tgaagaatcg tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa
     4441 agagagggcg ttacatattt cgctacactg acatcattga aacccatcac agaaatatct
     4501 tctggtacca ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca
     4561 ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt ttaaatactg cttcactgct
     4621 tgatatcctg actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt
     4681 tctgccaaaa cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc
     4741 ttcgtatatt ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaaatgt
     4801 tcaatgactt tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa
     4861 gtgccgtcag aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga
     4921 gcttcgtcga attttcctag tgcgatcgtt ccatctgtcg gaagatcact aatggaatcc
     4981 cacgtttctt tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctagacga
     5041 atagataaat aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga
     5101 agcgtttgct tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct
     5161 gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga
     5221 actcttgaaa cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc
     5281 atttgtctct cctccttttt tacgtttata aaattgcagt aaatcgttca aacgctgcct
     5341 gtcctttttc atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac
     5401 ctacagattt ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt
     5461 ttaattgttc tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga
     5521 aatgctctac ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta
     5581 cttcgatcaa cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa
     5641 tcccatcagg atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt
     5701 aattgtccct tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa
     5761 aagctctgat agatactgtt tcatccgaat atgctttcca cttttccaat actgagttag
     5821 ctgcttcaat caacaatttt ttctctgttg attgcaaacg gatcacagac atcggccatt
     5881 tcacgactcc tgcgatgact tgcggataat ctgctagttt gataagtttt tctatcggtg
     5941 cttttgccat aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc
     6001 ctccaacaat cggcaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa
     6061 tccgaagcag tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata
     6121 aaatgatcga atgttcattg taataagcat aaggagaata ttggaatccc cagctttcac
     6181 cgtctagatt cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc
     6241 tatatccttc gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc
     6301 gttctgctgc gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca
     6361 gttcaccata ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga
     6421 tgtagtcatt tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg
     6481 aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta
     6541 attgcgcttc aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg
     6601 ccttagcgac aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa
     6661 tgctttctag agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt
     6721 ccatttccat ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac
     6781 tgattgacat atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc
     6841 ccatgctgtt ttgataattg ctttgatatc tgtgtattca ggtttccaac ccagaacctc
     6901 tctcgctttt tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat
     6961 ttctgctgga atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga
     7021 gtatcctttg ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc
     7081 caaagctaaa atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt
     7141 tccatctggt gtatcataat cgtctccata aacagccaat gcttttcttt gaccaagagc
     7201 tacttgtaaa atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc
     7261 atctgctttt gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc
     7321 ttggtcacac cacttcatca ttttttccat catcaattta ctttcaccat acggattttt
     7381 aggattagct ggtgtatctt ctgtgattgg agactctttt ggttctccgt aagttgctgc
     7441 agtagaagag aacacgattt tattcacatt gaactcgtgc atgacttcaa gtaagatttg
     7501 cataccgtat acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa
     7561 agagcttgca gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag
     7621 aaattcttta tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt
     7681 gtgacctgtg agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc
     7741 tacagcgtgc gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac
     7801 tcctttgatt agtaaaa
//
LOCUS       DAEGIQ010000116         7699 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000116 DAEGIQ010000000
VERSION     DAEGIQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.119"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            544..1107
                     /locus_tag="KMT45_002374"
     CDS             544..1107
                     /locus_tag="KMT45_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7005816.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="KMT45_002375"
     CDS             complement(1386..1673)
                     /locus_tag="KMT45_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE7005817.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="KMT45_002376"
     CDS             complement(1663..1992)
                     /locus_tag="KMT45_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE7005818.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="KMT45_002377"
     CDS             2297..2626
                     /locus_tag="KMT45_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005819.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="KMT45_002378"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="KMT45_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBE7005820.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="KMT45_002379"
     CDS             complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="KMT45_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBE7005821.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="KMT45_002380"
     CDS             complement(4890..5498)
                     /locus_tag="KMT45_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBE7005822.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="KMT45_002381"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="KMT45_002381"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBE7005823.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="KMT45_002382"
     CDS             complement(6528..7496)
                     /locus_tag="KMT45_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBE7005824.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2352 a         1532 c         1280 g         2535 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tagttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatt
//
LOCUS       DAEGIQ010000117         7629 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000117 DAEGIQ010000000
VERSION     DAEGIQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.120"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(14..706)
                     /locus_tag="KMT45_002383"
     CDS             complement(14..706)
                     /locus_tag="KMT45_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBE7005825.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="KMT45_002384"
     CDS             complement(1143..1712)
                     /locus_tag="KMT45_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBE7005826.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="KMT45_002385"
     CDS             complement(1786..2109)
                     /locus_tag="KMT45_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005827.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="KMT45_002386"
     CDS             complement(2263..3075)
                     /locus_tag="KMT45_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005828.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="KMT45_002387"
     CDS             3429..4277
                     /locus_tag="KMT45_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBE7005829.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="KMT45_002388"
     CDS             4422..5363
                     /locus_tag="KMT45_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBE7005830.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="KMT45_002389"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="KMT45_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2474 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aaccttttt
//
LOCUS       DAEGIQ010000118         7610 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000118 DAEGIQ010000000
VERSION     DAEGIQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.121"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            209..2071
                     /locus_tag="KMT45_002390"
     CDS             209..2071
                     /locus_tag="KMT45_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE7005831.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWITGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQADQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2071..2403
                     /locus_tag="KMT45_002391"
     CDS             2071..2403
                     /locus_tag="KMT45_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE7005832.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
     gene            2423..2779
                     /locus_tag="KMT45_002392"
     CDS             2423..2779
                     /locus_tag="KMT45_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HBE7005833.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            2795..3145
                     /locus_tag="KMT45_002393"
     CDS             2795..3145
                     /locus_tag="KMT45_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HBE7005834.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMIE
                     DPNGIAIPFVTKKI"
     gene            3174..3953
                     /locus_tag="KMT45_002394"
     CDS             3174..3953
                     /locus_tag="KMT45_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HBE7005835.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            3964..4641
                     /locus_tag="KMT45_002395"
     CDS             3964..4641
                     /locus_tag="KMT45_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HBE7005836.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            4660..5766
                     /locus_tag="KMT45_002396"
     CDS             4660..5766
                     /locus_tag="KMT45_002396"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBE7005837.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMSLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQSENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            5756..6847
                     /locus_tag="KMT45_002397"
     CDS             5756..6847
                     /locus_tag="KMT45_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBE7005838.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            6860..7600
                     /locus_tag="KMT45_002398"
     CDS             6860..7600
                     /locus_tag="KMT45_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBE7005839.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQIVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         2527 a         1215 c         1714 g         2154 t
ORIGIN      
        1 taaaaaattt gaaaaagcgt ttgattattt atattacgac ttagtgcaat aaaaaacgct
       61 ttttaacagt acatgtagtc gtctttataa tagataaact ctgtagtatt agttaaaaag
      121 ctataagttg cacttagtta agtgcaaaca tttcatattg tcgttagttt agaaaacgct
      181 tatattagca atgtaaggag agatgataat gcagcagcta acaaaaagag aaaggcagct
      241 tttgatttac ttggtggaaa tgtctgaacc ggtaacaatc aaaaagttgg ctgaaaaaga
      301 aaatgttagt gttcggacga taaaatacga tctagatgat ataagagagt ggctgaatga
      361 gcggaagcaa gtcctatata gcaaacgtag tcagggaatt tggatgaata ttagtagctc
      421 tgatcgaatc aaattaaaaa gcgaattgat ggatgttaat cgttttgaaa tatttgcaga
      481 tcaaagtctc cgaatcaaac gtttactaat tatgctttta gtaacaaaag aagctattac
      541 tggaatgaaa atggccaacg cattagatgt cagcaaagat acgatcatga atgatttaga
      601 tgtgctggaa aataattttc ttaaggaagg attgaccttg aatcgtcagg cgcgaaaggg
      661 tttttggatc acaggagaag aacgtctagt tcgattaacg attgaagaga ttcttcaaaa
      721 agagttcacg gattatgaca tttataaatt aatgtcattg ttattgaatg gcggtgagac
      781 tgaatacttt gaaatgtatt ctgctactgc tacacctatc caagaggtat ttaatcaagt
      841 aatcattcga atgcggcact tactggaatt tgaaaatcta gagaaactga attacgcgga
      901 attactaaat attctgattc gagtgacaat tgctaccgtt cgcttacgca aagaggctac
      961 gattggtcgt tatcaattgg ttgctgaaca agaattagac aaaaacgatc tttcttatcg
     1021 cgtcatgcag caggtctttg attattatca gttgccactg ttcgaggatg aatatcatta
     1081 tatctatagt gatacattcg acacgattca ccagcaagac gtcatgaaac tagttgagaa
     1141 tgtgatttat aaagtaagta aagcaatcaa ccattctttt tatcaagatc cacaattgtt
     1201 gacgaatctg tatgctcatt tatccatgcg attatcgaga aagaaaaaat ttgtcaatga
     1261 atataatcct tttaaagaag atatcaagca gaaatacccg gaattatttg atgcgatcaa
     1321 gcaagcagtt cgacaagaaa taagtgaaca aaatattctg atcaatgatt ctttcgtggc
     1381 atatattgcc ttacactttt tggtttccta cgaaagagaa gaagatctac gaagcgttag
     1441 agccgtctac atttgttcaa cagggttggg tgtgactagt ctgatcaagc aaaagatcaa
     1501 tgaagaatta aataatatca ctatcgtagc atttgcttca gtattgaatg cgcaagaaat
     1561 gattgccacc aaagatcctg atttggtgat tagtattttt ccaatcaatg gcatcgatcg
     1621 tcctttcatc aaagtccatc cattacccac tgaacaggac ttgaaaaaaa ttcaacaaat
     1681 aataaaaaaa atacttgctt cttccaatca gcaggaaagg cataacttga atcttagaga
     1741 aacgtccatc aataaagata ctttagaaga ctttagcaag gaattgattt tgaaagctta
     1801 tacgatttat gaacgtttac ttgtcatttt ggaagcagaa ttgatccaag aatatcgaga
     1861 agcttttttg cttcatgtat tcttaatggt tcatcgtatc acatttgatc aacaatatat
     1921 ggcagaaggt cgtcaagcag accagattat tttagacaaa ggaacactgg taggaaaaat
     1981 cgaagcagtt tttgcagaaa ataatttagg aatcaatcag tcagaaatca gtgcattatt
     2041 ctcatatata aaagaaggag cgtcaagtta gtgaaaatgt cagacgaggc tttaaaaatt
     2101 atcgaggaaa gtcaaaatcg ggaaaagtta aaacaagtga ttgatctaat tgaagagcga
     2161 ttagtagaac atgctattgt accaaccgag ttgcagtgga ccattctaat taatcacttg
     2221 aatgagatgc tgaaacgttc tcaaagaaag gaaacgattc ccgtagtcga tcgggatttg
     2281 ttcaaagaag tatccaaaga agccataact atttctgatg aaatagttag aagtatcggt
     2341 aatttaaccg atgatgaaat atatgttttg tcgattcatt ttgaaacagc aaaaaataat
     2401 taatatttag gaggaaacaa tcatgattaa agtagttatt gcagaccgta tgggtaaagg
     2461 acaaaatgtg gccaaagggg tagaagcagc tggtggaaaa gcagttgtcg taccaggaat
     2521 gggggcagat atgcgtttgg gcgatgtgat gcaacaagag catgcagata tgggtatctc
     2581 tttttgtggt agtggtggtg caggtgcatt aacagcagca acaaaatatg gctatccaga
     2641 acgacatggt atgcgttcga ttgaagaagg gatcacagct atcaaggatg gcaaaactgt
     2701 tctaggtttc ggctttatgg atcaagagga attagggaaa cgtctgacag aagaatttat
     2761 caaaaagcat ggaaaataga aagagggaca tgaaatgagc gttcaaaaga caattcagca
     2821 aaaagttatc gtctccggta aaggggaaac aaagcaacat gcttttgcat cagcgttagc
     2881 agaaattcaa aacaaaataa tggaacaatc aaacgtatta ttgcgaatcg aaccagtcaa
     2941 tgtggatatt ttaaaggcgg tcgaaaacaa atacacagaa cgctttctat ttttcttctt
     3001 cccacggcaa cgtattagct atgaagtcga actctctgtt actgtggctg taacagaaat
     3061 aaaattggca gacatatcgt tcagccagca aatgattgaa gatccaaatg ggattgccat
     3121 tccatttgta actaaaaaaa tctaattttg aatcataagg gggaaagaat aacgtggatt
     3181 tcgtaatcat tctagtcaaa tcggtcatta tcgggggatt acttggtttt gcagcatctg
     3241 tcggggcagc tcgaatgttt catgcaccta atacacaagg tttaggagct tttagaacat
     3301 taggcgaaat gaatgcctgc atgggtgatc cagcttctca tttttcattt ggtttaggct
     3361 ttttctttaa tgcatgggct tcttcagttg gtgctggtgc attcacacaa gatgtgacgc
     3421 atcgaatcat tcccaattgg gcagcggcag cacttctagc aaaaaacaaa gatgtcaccc
     3481 agaccatgca tgatccgaaa aaaatgggaa tcattggagc ggttatcggg gcagtggtgg
     3541 tagccttctt aaacacaaca gctgcagcaa ttccagaggc gttacaagtg acggcggttt
     3601 ccgttttggt accagcagcg acgattctga tcaatacagt aatgccagtt attttttggt
     3661 tggcagcttt agacgcaggg aaacgaactg gtttttgggg cactcttttt ggtggtatcg
     3721 cgcaattgat tatgggaaat gcggtacctg gtgttgttct aggaatcttg attggtaaag
     3781 gcgtcgatga gagcggttgg aatcgcgtca cgaagagcat gatgatcgct attgttttat
     3841 tatttatttt cagtggattt ttccgtggat ttgatatgga aatgatcaag agcttccagc
     3901 ttcaagtgcc taattggctt gataacttgc acaacatctt tagtgtgaaa taggaggaga
     3961 atcatggaga caacagaaga acaagcaaaa gtattagaag caaagcggca aaagagcttt
     4021 tggtttgcag actggtcttt tccgctaatc gtaggaatta tgtccgctgc tgtatttgca
     4081 ggaacacaca tgtatgtgat gtatggtgtg ggtgcattca atgaagtttc gatcgtggcg
     4141 atgttaaaag ccgggctgga tggcggatcc tatggcgcag cggcagcgtt tggtgctagt
     4201 ttcttatttg ccagaatttt agaaggctct ttagttggga tcttggactt aggcggttcg
     4261 atcctaactg ggatcggaat tggggtacca gcaattctct taagtatgaa aatagcggca
     4321 ccaatcgata attttgcact ctctttattg actggtttgg ttctaggttt gattgttggt
     4381 ggcatcattg tcttgattcg taaatttacg atcaatcaat caaattccac ttttggtgct
     4441 gacgtcatga tgggggcagg aaattcatct ggacgttttt tgggtccatt aattatatta
     4501 agcgcgtgtg cagcatctat tccaatcggc attggcgcta cattaggagc actaggattc
     4561 tatgcttgga aaaaaccaat tgctggaggt gctattttag gagcaatgat tttaggagca
     4621 atcttcccgg cagtaaatta atagaaagag ggtcattaaa tgtttgatac tttgattaaa
     4681 aatggaaggt tggttgatgg atctaaaata gaggtcgcta tagaaaaagg catgattaaa
     4741 gcagttgcag cagaaataaa tcagccggcg caagaagtag ttgacttgga gggaaagtat
     4801 tatttatccg ctgggtggat cgatgatcat gtccactgct atgaaaagat gagtctttat
     4861 tatgactatc ctgatcagat aggggtcgaa aaaggtgtga cgacggtcat tgatgctgga
     4921 actacgggag cagaaaacat ccgtgaattt tatcaattga cgaaatcggt caagacaaat
     4981 gtgtatgcgc taatgaatat ctctaaatgg ggcattgtca aacaagatga acttgctgat
     5041 ctaacaaaga ttcaagaaga tctagtaagt actgcgttag cagaactgcc ggaatttatt
     5101 gtcgggatca aagcgagaat gagtaagaca gttgttggtg aaaatggcat taagccatta
     5161 gaattagcta aaaaaatcca gtcagaaaac aatgatctgc cattgatggt tcatatcggt
     5221 tctgcacctc ctgagttaga agaagtattg tcaagaatgg aaagaggcga tgtattgacc
     5281 cattgtttta atggaaaacc aaacggtatt ttagatccaa agacagatca aattaaaaac
     5341 tttgtctggg atgcttacaa taatggtgtt gtctttgata ttggacatgg gacggatagc
     5401 tttaatttcc atgtggcaga aaccgcacta aaagcaggaa tgaaagctac ctctattagt
     5461 acggatatct acatccgcaa tcgtacaaat ggcccagttt ttgatctagc aactacttta
     5521 gaaaaattga gggtagtcgg ctattcttgg gaagaaatca ttgaaaaagt tacagcagtt
     5581 cctgcacgga attttcattt gaagaataag ggccagttag ctgtcaatta tgatgcagat
     5641 ttgacgatat ttgagattgt tgaaaatgaa aaggaattga aagattccaa cggatttaca
     5701 cgtatagcta aagaacaaat tttaccgatt aaaacaatta ttggaggcgt catatatgac
     5761 aattagctat ggaaagtttg atttaaaaga agtcattaat gcatcgggaa aaatgacgat
     5821 cctaggggtt tctaaggtat ctgaaagtgt cctagcagca caacgatttg gaggcgagca
     5881 tttttttgag atgagtgatc ttgccatcaa aaccggcgct tatctttctc aattactgaa
     5941 agtggaagat gcgcaaatcg tttcttctgc ttcagcaggg attgctcaat ctgttgctgc
     6001 tttaatcgga caaggcgaca tgtaccatgt ctatcatccc tatacagaaa aaatcgctag
     6061 acgggaaatc atcataccta agggacataa tgtagattat gggacaccgg ttgaagtaat
     6121 ggttgctcaa ggtggaggaa aagtcataga agctggctat gcaaatatgt gtacaccgga
     6181 acatgtttcc atgatgatta cggaaaaaac tgctgcgctt ctatatatta aaagtcatca
     6241 tgcagttcag aaaagtatgc tgacggtggc agaaatggta gaagttgcca aagctcatcg
     6301 tttgccattg atcgttgatg cagctgcgga agaagattta ttcaaatata gcgaaatggg
     6361 cgttgactta gtgatttact ctggtgcaaa agcaatcgaa ggtccgagtg ccggactggt
     6421 cataggaaaa aagaagtata ttcaatggat tcggctacag tctaaaggta ttggccgagc
     6481 aatgaaaatc ggtaaagata atattttagg ctttacccag gcactagaag attatttaaa
     6541 aaatgggagc gaatccggtg actccatgaa gcaacgatta gcgccttttg ttaacgcaat
     6601 caatcaactt cctgaccttt cagcgaaaat agttcaagat ggtgccggca gagatattta
     6661 tcgagcaagt gtaacgatta atggcgataa aagtgcgaaa gaagtcattc aggaattaaa
     6721 agcacgaaat ccagcagtct acacccgaga atatcaagca aataacggaa tcattgagtt
     6781 cgacattcgt tccgtcagcc aaaacgaaat ggaaaaaatt ataaaaagat tgagagaaat
     6841 catgtaggag gcttttaaat tgtcagttat cccaaattat tataaaaatc gtgtatgttt
     6901 aaatgtatta gctcattcag ttgaaaatgc aaaagcatgc tatgaagcag cggaagggca
     6961 tgtcgtgcta ggtgtgctat cgaaaaatta tgctagcgat gaagcagcaa tcgaagatat
     7021 gaaaaggtac caagacgcaa ccaataatgc gttgtcagtt ggattaggcg ctggagatcc
     7081 aaaccaaagt cagatagttt cgcgtttatc tggggtttta caaccacaac atgtcaatca
     7141 agtatttact ggcgtgggga cttctcgtgc tttattgggc caaaatgata cagtcatcaa
     7201 tggattggtt tcaccaacag gtaagatcgg ctatgtcaat attgctacag gtccgttaag
     7261 ttcacaagcg ccagcagctg aagtgcctat tgaaactgcc attcaaatgc tgcaagacat
     7321 gggaggtact tcaatcaagt atttcccgat gaaaggctta gcacataggg aagaatttaa
     7381 agcagttgcg gaagcttgcg caaaatatga attctattta gagccaacag gaggaattga
     7441 tctttcaaac tttgaagaaa ttctgaaaat tgcaatcgat gcaggtgtaa aaaaagtgat
     7501 tcctcatgtt tatagttcaa ttatagataa agaaacagga gatacgcgta taggagacgt
     7561 aaagatacta ttaagcatgg taaaaaaaat tattcactaa ctcattagaa
//
LOCUS       DAEGIQ010000119         7600 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000119 DAEGIQ010000000
VERSION     DAEGIQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.122"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(943..1356)
                     /locus_tag="KMT45_002399"
     CDS             complement(943..1356)
                     /locus_tag="KMT45_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBE7005840.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="KMT45_002400"
     CDS             complement(1812..2297)
                     /locus_tag="KMT45_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005841.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="KMT45_002401"
     CDS             complement(2436..2651)
                     /locus_tag="KMT45_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBE7005842.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="KMT45_002402"
     CDS             2875..3675
                     /locus_tag="KMT45_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE7005843.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="KMT45_002403"
     CDS             3694..5562
                     /locus_tag="KMT45_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBE7005844.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="KMT45_002404"
     CDS             5555..6046
                     /locus_tag="KMT45_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBE7005845.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="KMT45_002405"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="KMT45_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBE7005846.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="KMT45_002406"
     CDS             6606..>7600
                     /locus_tag="KMT45_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBE7005847.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAEGIQ010000120         7502 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000120 DAEGIQ010000000
VERSION     DAEGIQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.123"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(108..1406)
                     /gene="eno"
                     /locus_tag="KMT45_002407"
     CDS             complement(108..1406)
                     /gene="eno"
                     /locus_tag="KMT45_002407"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBE7005848.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1522..2277)
                     /locus_tag="KMT45_002408"
     CDS             complement(1522..2277)
                     /locus_tag="KMT45_002408"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBE7005849.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2381..3571)
                     /locus_tag="KMT45_002409"
     CDS             complement(2381..3571)
                     /locus_tag="KMT45_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBE7005850.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3681..4682)
                     /gene="gap"
                     /locus_tag="KMT45_002410"
     CDS             complement(3681..4682)
                     /gene="gap"
                     /locus_tag="KMT45_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBE7005851.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4723..5814)
                     /locus_tag="KMT45_002411"
     CDS             complement(4723..5814)
                     /locus_tag="KMT45_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBE7005852.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5963..7297)
                     /gene="rpoN"
                     /locus_tag="KMT45_002412"
     CDS             complement(5963..7297)
                     /gene="rpoN"
                     /locus_tag="KMT45_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBE7005853.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7357..7428
                     /locus_tag="KMT45_002413"
     tRNA            7357..7428
                     /locus_tag="KMT45_002413"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7390..7392,aa:Arg,seq:ccg)
BASE COUNT         1940 a         1587 c         1387 g         2588 t
ORIGIN      
        1 ctctctacct gctgatattt cagcaagtag agagttcgat tacttaacta agtgatcata
       61 ctcgactatc tttcaatagt cgatcatgta tcttcattag aaaagaatta tttgtttttt
      121 aagttgtaga aagatttcaa acctttgtat tctgcaactt caccaagttg gtcttcgata
      181 cgtagtaatt ggttgtattt agcaatacgg tcagtacgtg atagagaacc agttttgatt
      241 tggccagcgt ttgttgcaac agcgatatca gagattgttg aatcttctgt ttcaccagaa
      301 cggtgagata caactgctgt gtagccagct tctttagcca tttcgatagc ttcgaaagtt
      361 tctgttaatg taccgatttg gttcactttg ataaggattg agttagcaat tcctttttcg
      421 ataccttcag ataatttttg agtgtttgtt acgaacaagt cgtcaccaac taattgaact
      481 ttgtcgccta atacgtcagt taatttcttg aatccgtccc agtcgttttc gtctaatcca
      541 tcttcgattg agatgattgg atatttagaa actaattctt cgtagaattt gatcatttcg
      601 tcagttgttt tttcgccttc gcctgagtca gctagtacgt aaacaccttt ttctttgtcg
      661 tagaattctg aagaagcagc atccatagca agaacaacgt ctttaccagg tacatagcca
      721 gctttttcga ttgcttcgat gattacttca aaaccttctt cgtttgaacc aaggtttgga
      781 gcaaatccac cttcgtcacc aacagaagtt gctaatccgc gagctttcaa gattgaagct
      841 aatgcgtgga atacttcagc acccatacgt agagcttctt tgaatgttgg agcgccaaca
      901 ggcatgatca tgaattcttg gaagtcgatt gagttatcag catgtgatcc gccgttgatg
      961 atgttcatca ttggtgttgg caatactttt gtgttgaatc cgcctaggta gtggtataaa
     1021 ggtacttcta ggtagtcagc agctgcacgt gcaacagcga tagaaacacc taaaatagcg
     1081 ttcgcaccta atttaccttt gttaggagtt ccgtctaaag cgatcatagc tttgtcgata
     1141 gccatttggt cacgaacatc gtagccaatg attgcttcag caatgatgtt gtttacgttg
     1201 tcgacagctt tagttacacc tttaccgcca taacgagctt tgtctccgtc acgtaattca
     1261 actgcttcgt attcaccagt tgaagcacct gaaggaacca ttccgcggcc aaaagctccg
     1321 ctttctgtgt atacttctac ttcgattgtt gggttaccac gtgagtctaa gatctcgcga
     1381 gcgtaaacat cagtaataat tgacatgttt tgtctctcct ttgagtttgt tttttcctaa
     1441 gggatttccc tcatatacga ttttagttaa ttttgctcat tagtgcaaac atttcttgca
     1501 tttttcgcaa attttcaaca attattttac agcgtctaac aatgacaaga atgaatctgc
     1561 ttgcaagctt gcgccaccga ctaaagcacc gtcaacattt tcttttgcca tgtattcagc
     1621 aatattttct ggttttacag aaccgccgta ttggatacga actgcttctg atacttcttt
     1681 tccataaagt ttttcaactg tttgacgtac aactccacaa atttcgtcag cgatttgtgc
     1741 gtcagctgat ttaccagtac cgattgccca gattggttca taagcaataa ccatgtttga
     1801 cacctgttca gctgaaagat cagctaaacc agctgtaatt tgtccttcga tccattcagc
     1861 agttttgcct gcttcatagg tttctaaaga ttcgccgcag cataggattg gtgtcatacc
     1921 atttgcaaag atagctttcg cttttttatt gatgtcttcg tctgtttcat ggaaatattc
     1981 acgacgttct gaatgtccga tgatcacata atgaacacct agatcagcaa gagctgctgg
     2041 tgaagtttca ccagtgaacg cacctgagtt ttcccagtag cagttttgtg ctgagatttt
     2101 tagttctgta ccttcagctg atgtcattaa ttcttgtaag aataatgcag gagatccgat
     2161 gactgaatca actttgtcat ttgaagggat tttgtttttt actgcttcag caaattcttt
     2221 tgcttctgat gcagttttgt tcattttcca gttacctgcg ataataggtt tacgcattga
     2281 ggaacacact ccttaattta ttgaaaaatg gctagggagt catgaaaact cccttcacct
     2341 tttgttcgat tactcgtcag aattgagcaa acttggtcgt ttatttatcg ttgattgctg
     2401 ctagtcctgg taattcttta ccttcaagca attccaagct tgcgccgcca cctgttgaga
     2461 tgtgtgtgaa tttgtcagca aatcctaatt gttctgctgc tgcggctgag tctccgccac
     2521 cgatgattgt tgttgcgcct tctaagttag cgattgcttc acaaacaccg attgtacctt
     2581 tagcaaagtt actcatttca aatacaccca taggtccgtt ccaaacaact gtttttgcac
     2641 cttgtaatgt atcagcaaat aattttactg atgctgggcc gatgtctaaa gccatcaaac
     2701 catcaggaat gtctccttcg attacttgtg tttcaacatc atttgaaaat tctggtgcac
     2761 agacattgtc gattggcaag acaagtttgt cgcctgcttt ttcgattaat tcttttgcta
     2821 gttccacttt atcagcttct actagagagt taccgatctt gattcctttt gcttcgtaga
     2881 atgtgtaagt cattccgcca ccgatcaaga ttttatctgc tttaggaatc aagttttcga
     2941 ttacgccgat tttatcagaa acttttgcgc caccaaggat tgcaatcatt ggtcgttttg
     3001 gttcttctac tgcttcaccg atgaacttga tttctttttc catcaagaat ccagcaactg
     3061 ttgggatacc agttgaagcg atacctacgt tagaagcatg tgcacggtga gctgtaccga
     3121 aagcatcgtt aacaaataca tcgcctaatg aagcccagta tttacctagt tctgtatcat
     3181 ttttgctttc ttttttaccg tcaatatctt caaatcgtgt attttcgaat acgacaacgt
     3241 cgccgtcatt catgttgcgg attgcttctt ctaattgttc tccgcgagtt tcaggtacga
     3301 atgttacttc tttacctaat aattcgccta gacgttttgc aactggagct aaagattttc
     3361 cttctttatc ttcttctgtt tttacacgtc caaggtgaga gaaaaggatt gcttttccgc
     3421 cttgttctag aacgtagttg attgttggca atgctgcttt gatacgagtg tcgtcagtga
     3481 tcacgccgtc tttcaaagga acgttgaagt caacacggac aagaactttt ttgtctttta
     3541 aatcgatatc tttcactgtt ttcttagcca ttttatttcc tcctgaataa acttattatc
     3601 aaaaaaagcg gaagaagcgc gattgcttcc ccgcttttaa atgtacatgt ctataattta
     3661 cacttgtacg tttgtctatc ttataagtta gcgaagtatt ctaaagtacg tactaattgt
     3721 gcagtgtatg acatttcgtt gtcgtaccaa gcaacagttt taactaattg tttgtcgcca
     3781 actgtcatta ctttagtttg tgtagcatca aataatgaac cgtaagtcat accaacgata
     3841 tctgaagaaa cgatttcgtc tgtgttgtat ccgtaagatt cgtttgaagc ttctttcatt
     3901 gctgcattga tttcgtcaac agttacttct ttttcaagaa ctgttactaa ttctgttaat
     3961 gaacctgttg gtacaggaac acgttgagca gctccgtcta atttaccgtt caattcggga
     4021 attactaaac cgatagcttt agcagcacct gttgagttag gaacgatgtt tgcagcagca
     4081 gcgcgtgcac ggcggaagtc acctttagga tgaggtccgt ctagagtcat ttggtcacct
     4141 gtgtaagcgt ggatagttgt cattaatcct tcaacaacac caaatttgtc gttcaatgtt
     4201 ttagccatag gagctaaaca gttagtagta catgaagcac ctgagataac tgtttcttta
     4261 ccagttaatg tttcgtggtt tgtgttataa acgattgttg gaacatcgtt tccgccagga
     4321 gcagagataa caacgcgttt agcaccagct gttaaatgtt tttcagctgc tgatttagaa
     4381 gtgaagaaac cagtacattc tagaacgata tctacgccta gttcgcccca tggtaattct
     4441 tctgggttgc ggttagctaa tactttaact tcttttccgt ttacgttgaa tgaaccttca
     4501 tgaacttcaa ctgttccgtt gaaacgtcct tgagttgtgt cgtattttaa caagtgtgcc
     4561 aacattttag catctgtcaa gtcgttgatt gctactactt cgattccatc tacatcttgg
     4621 atacgacgga atgctagacg tccaatacgt ccaaaaccat taatacctac tttaactgtc
     4681 atttaagatt tcctccttat gaaaatcaaa aaaatattta ttttaaaggg ttaccccttt
     4741 taaaatctca tttgcagcag cttcatcagt gatgagccac gtttgttttg gtgcattttt
     4801 catataagct cgaattgctt tagcttttga ttttccgccc gcgattgcca tgatgatcgg
     4861 gactttttcc aaatcttgca attctatgcc gattctaggg accttataaa cgacttcacc
     4921 tttttcattg aaaaagtagc caaaagattc cgcaacagca tttgcttgtt tcagcatgac
     4981 taattctttt tcagtcatct tacgacgtgc tgccatgtgt aatgcacgcc caatactatg
     5041 aataacacaa ttcgcttcac taatcaagtt taagacttct tggatagatg gctcattgag
     5101 taaagaattg taagttgcca gacttaattg ttccggaaca taaagtgcgc ggtggttacc
     5161 gtttgcttta gcagccattc ttgcactgac agaatttgcc tgtacagtta tcgcttcacc
     5221 aatcccgcct cttgcaggga caaacaggtt atgccgtttc ttattttcca gattagacaa
     5281 ttgttcggct accacagcca tagtagttcc tcccatcaca gcgatgatat tctcgccttc
     5341 tggaagtagt cggtctaagg cttcgctcag cgcttcgccg aatgcatcag ccactttggt
     5401 ctgctcttcg ctgtttcctg aaacaacgat acagcgttgg ttaccaaaat attctgccaa
     5461 ctgttgttcg gtttgatgcg tacctaaaag ctgatccatg aagttttcaa ggctttgata
     5521 aacttcttcg cccttagttg tcagcgtcat accgcttttt gaggtgtcga tcaaattcag
     5581 atttttcaaa aggtctgttt ctgttcgtaa gactcgttct gtgatcccca tactttccga
     5641 taaacttcga cgcccaatcg gctgcatcca atagatattt cgtaaaattt tatagcgttc
     5701 ttgcaacact tcgataatat ctggtgcaac agattcaatc atttttattt catccagcat
     5761 agcgatctcc ttagttggac actttacgtc caatgtagac ttaaaacgac ccatatacga
     5821 taaaaaaaga aattaactat tcgaatttca catcaataaa gggaaatctc aacctcacga
     5881 ctgtaagtct aacagttttg ctctcgtttt tcaactaaaa agactagaaa aagtgaaata
     5941 ctttttctag tcttttgact gattagttat cataacgttt tctttttgat gaactcggga
     6001 ttcctaataa atcacgatat tttgctactg ttcgtcttga gatcgctatc tcatcttttt
     6061 tcagcaattc aaccaatttc tggtcagaca acggcttgtt tttgtcttct tgatcgacta
     6121 attcctgcag ctttttcttc gctgtatctg ctgaaaggtc ttctgtctgt tctgtactat
     6181 tcgtaggaat tcgtgtagtg aaaaattttt ttaattcaaa tacaccgaag tctgtttcca
     6241 aatatttccc gttgactgca cgactgaccg tggattcatg cacatctatc tcagttgcaa
     6301 cttcctttaa cgtaagaggc ttgattggac gttccttgtc gataaagaaa gctttttgcc
     6361 gggaaacaat gacttgagca acccgcaaaa ttgtatcccc acgttgtagg atcgttttct
     6421 tcaaccattc gaattcctgt ttccgttctt ttaaataatt ttcagtttct ttatctgctt
     6481 gttgtgacat ttgtttgaag taactctctt gaaagcgtat atttggctga ttttttcggt
     6541 ttgagataac ttgtacattt ttatcgtcgt ccactaatac ccggacatca ggaataatgt
     6601 acaattcgct gctacggtcg aaaatccggc cagggcttgg actcaacgtt tgtaaatagt
     6661 cgaatatctg ctgaacagca tgtagatcaa cagaaaaacg ttgtgcgatt tctttccatt
     6721 ttcgctccac cagttcctca aaacattctt ccaacacgat ataagctaat tccggcgcat
     6781 aatcatctcg ttctgtttga agcatgagac attcttgcaa actacgtgca cctactccag
     6841 ctggttctaa ttgctgaatc agagtcaacg catcgagcat ttggataggc gtagctccaa
     6901 tctcttttcc ggcttcttct aaactaatcg tcaaaaatcc gttcaaatcg atgtattcta
     6961 caagataaag aacgattttc ctcaaaaaag tatctcggta attcaaatgg acttgttcga
     7021 tcaatgaatc aaataaagac ccttttgtat caggaatctg atttaggtaa tttgtctctt
     7081 ctccttcata agaagatgaa gaagctttta tgtaatctgg ctgccattcc ggctcaacaa
     7141 cttccaccaa tggattttcc attgcttgat tctcaacgaa tgccaacagc tcctcactgt
     7201 tatattgtaa aacttggatg gactgttgca actgttgcgt catggctagt ttctgtgtct
     7261 gctgctgttt ttgtgaaaaa ttttgctcaa atttcatttt tttgtccaat ccccttgtaa
     7321 ttttttatct gtttagttac aattatccca gtgcttgcgt ccttagtgta gtggatatca
     7381 cgtaagattc cggttcttga gacgggggtt cgattccctc aggacgcgtt ctactgctat
     7441 tttagcataa tttacgaata gttgcttctt tatttttaag tgaaaactgt ccgccaaaga
     7501 aa
//
LOCUS       DAEGIQ010000121         7454 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000121 DAEGIQ010000000
VERSION     DAEGIQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.124"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..960
                     /locus_tag="KMT45_002414"
     CDS             <1..960
                     /locus_tag="KMT45_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBE7005854.1"
                     /translation="YTYEETTILTQVQQLQLKAIGKVPVDLGWKNLFGKESDGKEKEE
                     EPLLPKVAKGEMVTVDLQVLEKETKPPKPYTEGTLITAMKTAGKTVDSEEAQSILKEV
                     EGIGTEATRANIIETLKQKEYIQVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWE
                     SYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGATLQEVKAASEKQEAAR
                     HLGICPKCQEQEVILYQKVAACTSEACDFKLWTTIAKKKLTATQLKEIIQNGRTSQPV
                     KGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            976..1125
                     /locus_tag="KMT45_002415"
     CDS             976..1125
                     /locus_tag="KMT45_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005855.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            1206..1802
                     /locus_tag="KMT45_002416"
     CDS             1206..1802
                     /locus_tag="KMT45_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBE7005856.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGNIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1815..2714
                     /locus_tag="KMT45_002417"
     CDS             1815..2714
                     /locus_tag="KMT45_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBE7005857.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2717..3190
                     /gene="ssb"
                     /locus_tag="KMT45_002418"
     CDS             2717..3190
                     /gene="ssb"
                     /locus_tag="KMT45_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE7005858.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            3556..3816
                     /locus_tag="KMT45_002419"
     CDS             3556..3816
                     /locus_tag="KMT45_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005859.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            4261..4467
                     /locus_tag="KMT45_002420"
     CDS             4261..4467
                     /locus_tag="KMT45_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBE7005860.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4467..4718
                     /locus_tag="KMT45_002421"
     CDS             4467..4718
                     /locus_tag="KMT45_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005861.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            4734..5150
                     /locus_tag="KMT45_002422"
     CDS             4734..5150
                     /locus_tag="KMT45_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005862.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            5132..5767
                     /locus_tag="KMT45_002423"
     CDS             5132..5767
                     /locus_tag="KMT45_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005863.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFISYTICRKHYYSES
                     EYYSIFESMELMEELNSKDATVDDYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLRE
                     YVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKQMPYQRFERKKGQ"
     gene            6148..6327
                     /locus_tag="KMT45_002424"
     CDS             6148..6327
                     /locus_tag="KMT45_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005864.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            6737..7138
                     /gene="tnpA"
                     /locus_tag="KMT45_002425"
     CDS             6737..7138
                     /gene="tnpA"
                     /locus_tag="KMT45_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBE7005865.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            7150..>7454
                     /locus_tag="KMT45_002426"
     CDS             7150..>7454
                     /locus_tag="KMT45_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005866.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRP"
BASE COUNT         2711 a         1233 c         1493 g         2017 t
ORIGIN      
        1 tatacctatg aagaaacaac gatcttgacg caagtccagc agcttcagtt aaaagcaatc
       61 ggaaaagtgc cggtagatct aggttggaag aatttatttg gaaaagagag cgacggaaaa
      121 gagaaggaag aagagccgct acttcctaaa gtggcaaaag gagaaatggt gacggtagat
      181 ctacaagtcc ttgaaaaaga aaccaagcca ccaaaacctt ataccgaagg tacgttgatc
      241 acggcgatga aaacagcagg gaaaaccgtg gatagtgaag aagcgcaatc catattgaaa
      301 gaagtggaag gaatcggtac agaagcgaca cgggcaaata ttattgaaac tttgaaacaa
      361 aaggaatata tccaggtaga gaaaaataag ctcgtggtga caaataaagg gattttactt
      421 tgccaagccg tggaaaaaga gccactgctt acgagcgccg aaatgaccgc caaatgggaa
      481 agctacttgt taaagattgg agaacgaaaa ggaacacaaa ccacttttct agccaatatc
      541 caaaaatttg tctctcactt attggaagtc gtcccaggac aaatccaatc cactgatttt
      601 ggagcaacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta
      661 ggaatttgtc caaaatgcca ggaacaagaa gtcatacttt atcaaaaggt ggcggcttgc
      721 acgtctgaag cctgtgattt caaactatgg acaacgattg ccaaaaagaa acttacagcg
      781 acacagctga aagagatcat tcaaaatggc cgaacaagcc aaccagtgaa agggttaaaa
      841 ggtcagaagg gttcgtttga agcaacgatt gttttaaaag aagacttcac aacgagcttt
      901 gaattttctg aaaagaaaaa aacgaactat aagaaaagaa cacgacgcac gacaaaataa
      961 tggagggttt atcttatgcg aaatacaaca cacaaacaac aagaaatcga aaatcaaaaa
     1021 gccaaaattc gttcagaaca ggaaaaaatg cgtgccagca aacgaagact gcagcaattg
     1081 aagaaccagc agaggattca aaaagaaatc gaattagatc gataaaatct tcctaactat
     1141 tgtatacgta taaacttatg ttatactgtt tttataagtt tatacgtata caaaggaggg
     1201 agaaaatgac aaaagcaatg gtatacaagc tgctagaaga atttaatgga actttggctt
     1261 tgaaggatgc caaaaaagcg ggcatttctc ctgtaacgat caaacgtatg gtagatcgag
     1321 gggaactgga tcgagagtat ccaggttttt tcaccttacc tggtcaattt cctgatgaat
     1381 tgtttatggc gcaaaaaaaa tatgaacgcg gcattatttc acatattacc gcgttagatt
     1441 tgtatgattt aacagatatg atccctagac agatcgatct aaccgtacct tacggttatc
     1501 acgtttctga aaaaggattg aaggaattcg cggttgagtt acactattca aaagcggaat
     1561 ggtatgaatt aggcaatatc gaaataaaga gtcgctatgg gaatcctgtt atagcttatg
     1621 atccagaaag aaccttgtgt gacatttgga atccctggta taacgttgag gatgaaatca
     1681 aagtaaaagc aatcaaaaac tacatggagt cggacagaaa aaatcttaga aaattgaatg
     1741 agtatcgacg aattttgcct acagataaaa cgatgcgctc ttatattatg gctttaaatt
     1801 aaaggagaag acacatgaca cctacgcaat taaaagcaaa agtgaagaat atttctagag
     1861 aaaaagaagt tgatccgcag cttgttttaa gacattttat gatggaaaaa ttcttggaaa
     1921 aaatctcaga gtctccttat cgtgaaaatt ttgtgttaaa aggcggcttc ttaattggct
     1981 caaagtatgg cattgaaaat cgaacaacta aagacattga tacgacttta cgggaaatga
     2041 aagtaaccaa agaaacattg acaacggttc taaacgatat tttttcaacg ccgacgaaag
     2101 aaggcattca atttgagatt caaggaatga aagaaacgag ggaagctgat tattatccag
     2161 ggtttagctt acgtgtactg gcacatttgg aaaacatgcg gccagatttt aaggtagatg
     2221 ttactacagg tgactcgatc tatcctgcaa cgatcactca tagtcataaa ttgatgtttg
     2281 aagatcgaac gattcctttg gaatcttatc ctacagaaca aattattgcc gaaaaattaa
     2341 gtgcaacctt tgatttttta acagataatt cgagaggaaa agatttttat gatctctata
     2401 ctattcctaa aatggagaag atcgatgaga agaccttgaa agatagtgta agaaatacat
     2461 ttacacgtcg aggaaaaact gatccgttaa agatttacta tgaaaaggat atggatgtac
     2521 tgaaaaatag tcacgaacta aaagatacct ggaagaaata tcaaaaaagc aatccttatg
     2581 ctgcgtcgat cacgtacgaa gagacaatgg attcaattag taatttgatg gaaaaagtga
     2641 ttcacgtcga agaaaaagag cgaaaactag cttatcaaag acaccaacag atgaatagag
     2701 aaatggagag ataaagatga ttaataatgt gacattagta ggacgcttaa ccaaagatcc
     2761 cgatttacgt tatactgcaa gtggcacagc agtagcgact tttaccctag ccgtcaaccg
     2821 aaatttcgcg aatcaaaacg gcaatcgaga agccgatttt attaactgcg tgatttggcg
     2881 caaaccagca gaaacaatgg cgacattagc taaaaaaggc ggcttgatcg gtgttgtcgg
     2941 acggattcaa acacgaactt atgacaacca gcaaggacaa cgtgtgtatg tgactgaagt
     3001 tgtagcagat aactttcaac tattagaaag taaagcagcg acagagagcc gtgcgcacgc
     3061 tgatcaatca accaccagtc caagccccac tacctttgaa caacgggata caaacaatgg
     3121 attggacgct tcacaaaatc catttggtgg acaatcaatc gatattagcg atgatgatct
     3181 accattctaa gtagcatttt ctattggttg catttgctac ggtttatgtt ataatttagt
     3241 caacaaaaag aattttcaat ctataaccaa aaaccactag ctggcgggct agtggttttc
     3301 ttttacgttt ctattataag aattaacggg taggcttact gtctttcttg gcaaatttac
     3361 aatcaagcca tttgcagata tagtgcgcgg ctacttctgc catgattgca aaaacaaaat
     3421 caaaaagaat tttcaaacct ataacctcct ccccttgcca gattaggccg gggtagcaga
     3481 cagcaagact attatagcat aattgaaata ttcaaatgat tggatttttg ctataatttt
     3541 agcgaggtga ttgtgatgaa caaatatgtt ggtttgcttg ataaaatccg agtaataaaa
     3601 acgcaaccgt tattggttcg ctttacttta caaacgattc atgaatcgat taattgtgta
     3661 gttgcagata ttgaaattat cgataaattg ctaattatgg atgatggaaa atacaatatt
     3721 gccgtcacag gtcacttcaa taaacgaaac cagctagtta ttgctagcat gtatgttaga
     3781 aatcccgatc agtttacgcg attgatgggc atataaaaaa agaagagttg caatcctaat
     3841 gtaggactgc agctcttttt gttttacata gaaacaagaa aaatgaaaag gcctttttag
     3901 gaaggcgcga gcgccttctc aattgaaaaa cctctctcac tccaaccgcc accgccgttc
     3961 gagaagaaag gaagtgaacg ttgcacgttc actaagataa agggtcttgc aaagcaagac
     4021 aaaatagaaa aaacttcctc tcaccaaaag gttcgttcct gttcgttatc tctttattga
     4081 tttgccagac cggcaacaag ttgcctttta tcaccaaaaa gcgacaagga caagggtaaa
     4141 tggcacgctg cgcgcccttg tccttgtcgc ttttcgttga ttcattctgg ccaacaaatc
     4201 aataaagaaa gaactaacga aaggaagtaa aacctttcct aagaggaggc agaatcaatc
     4261 atgcgaaaga aatttgatta ttggggtgtt cccttcagcg aattattccc aaactatcat
     4321 gcaccacata ctgtcgaatg cgattgtgga gaaagagcga aatgtatcaa aagctatcgt
     4381 ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt cttatggcga ttatattttg
     4441 attgatgaga aaggaaagaa acgttgatga aaacacaaca caaattattg actgtaaaag
     4501 aagtcgttca aattttggtg gacaaagaag tcatttctga aaaagtagca gaagctttac
     4561 ttattccgcg gtactggacg acagaaaccc ctttagtaga ggatgcaccg actatgctat
     4621 ttgaagacct agtgaagatt gcaggatatg aactgcatta tcaagaaaac ttggaaactt
     4681 atgaaaacga atattggtta accgaattag agaactaaaa aggagaaaaa aatatggctc
     4741 aaaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa aaaatggagg
     4801 atatgacatt cctatacaat gagacacgtg ttccaaaaaa acattataag gaaagattgt
     4861 cggtagctgt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt gcgacgtatt
     4921 atcgaacact ggaagaactg aaaaagcaaa atgcaaaatg gtttttccaa gccttactat
     4981 gcttggaagt aggcgtcaaa ccaagcacaa tcaaaccttc tgaataccaa gctttagaac
     5041 taacgtatgc gaaatttatc gaaacgaaaa aagctaagac tgtttcaagt gaatggttag
     5101 attattttga aaatatcaat aagtatggcg catgttacat catgaagtaa tcagacggat
     5161 tcaaaaagac tttagttgta tggaagaaat gaagtacttt gttcaaaatg ttgaagaaca
     5221 gttatatttg gaaagcgaaa cgagtcaaac aaaggtcaca gtcagccata atcgtttaga
     5281 tgtgtataaa cgtcgtcctg aatcattaaa aaaaggagcg gagcttctac gactagaaac
     5341 agaaaaatac gtcatgaatg tttacctaga aaaaagtgag ttggtcgaaa attttattag
     5401 ctacacgatt tgcaggaaac actattactc agaatcagaa tattattcaa tttttgagag
     5461 catggaatta atggaagagt taaacagtaa agatgcgacg gtagacgatt attaccttgt
     5521 atttactaga tttttaacgt gtggaacgga atctatcgtg ctaatgggaa tggattacga
     5581 actcgctgta cccgaagcgt ttttgaggga atatgtagaa gaagtagata acccagggga
     5641 tgattggtac acgactttaa ctgatttctt agacacctac acttacgacg atacagaaga
     5701 aatccaaaat gagcttgacc gtaaacaaat gccatatcaa cgatttgaaa ggaaaaaagg
     5761 tcaataatca tttataccga aaagtgagaa tgtgtgaaat aagaacaaaa aaccttgaaa
     5821 ttaagggaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
     5881 gcgaaataag cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa
     5941 atcattcctc tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct
     6001 gtacatttaa acttcgtagt attcttagga atactgatat tttgtttgat cttttggcgc
     6061 tttgtgttag cccttgtgag tctaatattc aagttgattg cagccatatt tttcataggg
     6121 ttatttcgat gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc
     6181 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa
     6241 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
     6301 tttgatatac cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata
     6361 gcacttaatt gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat
     6421 caataactat gaaatcttta aagtatccag aagagattat ttttagatca atttttttct
     6481 tacctgattc taaagatttt gttttcttaa acatattatg atttcctttc gactattttc
     6541 atataaatgt taacatagtt tatggactag ccgagaacac ccgtgatttt aatcatggga
     6601 tgaatcggat tcagttgtga cctagcttta gctaggttaa acaactgaca aatataaact
     6661 aaataatagt gaaatgtctt gcaataattg attttgtaga aatacatata ttaaaataat
     6721 tgcgaggtgt ttttgtatgt cagaaattca ttatggacgg ggttatgtct attctattca
     6781 atatcatctt gtttggtgtg taaaatatcg acatgatgtt ttgtatggtc aggtagatat
     6841 tgatgtcaaa cagtttttga aacaaattgc tgatgataat aatataaaaa ttatagaaat
     6901 agaatcagat aaagaccata ttcatttact cattgaatgc accccacaac actatattcc
     6961 tagtattgtt aaagtattta aaggcgcatc cgctagactg ctgtttaaaa aacatcctga
     7021 acttaaacaa ctcctttggg gtggacatct ttggaatcca agctactttg tagctactgt
     7081 tagcgaaaat acagaagaac aaattagaaa atatatacaa aatcagaaaa agaaatgagg
     7141 tgaatccgta tgctacaaca tagggcttat aaatttagaa tctatcctaa tcaagaacaa
     7201 gaaatattaa tgactaaaac tattggctgt tcaagatttg tctataatta ttttttgaat
     7261 ttgtggaatc atgaatatac gactactgga aaaggattat cttataattc ttgttctgcc
     7321 atgcttccac aaatgaaaag aaatgcagag acaatttggt taaaagaagt tgacagtatt
     7381 gcgattcaat cctcattgaa aaatctttct gatgcttttt ctcgcttctt taaaaagcaa
     7441 aataaacgac caca
//
LOCUS       DAEGIQ010000122         7397 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000122 DAEGIQ010000000
VERSION     DAEGIQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.125"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..142)
                     /locus_tag="KMT45_002427"
     CDS             complement(<1..142)
                     /locus_tag="KMT45_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7005867.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="KMT45_002428"
     CDS             complement(183..437)
                     /locus_tag="KMT45_002428"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE7005868.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="KMT45_002429"
     CDS             complement(548..802)
                     /locus_tag="KMT45_002429"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7005869.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="KMT45_002430"
     CDS             complement(995..1270)
                     /locus_tag="KMT45_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBE7005870.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="KMT45_002431"
     CDS             complement(1242..2195)
                     /locus_tag="KMT45_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBE7005871.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="KMT45_002432"
     CDS             2807..4300
                     /locus_tag="KMT45_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBE7005872.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="KMT45_002433"
     CDS             4435..4731
                     /locus_tag="KMT45_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBE7005873.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="KMT45_002434"
     CDS             complement(4755..5714)
                     /locus_tag="KMT45_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBE7005874.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="KMT45_002435"
     CDS             5897..6748
                     /locus_tag="KMT45_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005875.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..7052
                     /locus_tag="KMT45_002436"
                     /pseudo
     CDS             6769..7052
                     /locus_tag="KMT45_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DAEGIQ010000123         7230 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000123 DAEGIQ010000000
VERSION     DAEGIQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.126"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..152)
                     /locus_tag="KMT45_002437"
     CDS             complement(<1..152)
                     /locus_tag="KMT45_002437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005876.1"
                     /translation="MDKKAAMKRIAELTKSESWQEDKEIVAEVQRIGKSMWTEKPKRK
                     TPRKIA"
     gene            complement(155..316)
                     /locus_tag="KMT45_002438"
     CDS             complement(155..316)
                     /locus_tag="KMT45_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBE7005877.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(331..690)
                     /locus_tag="KMT45_002439"
     CDS             complement(331..690)
                     /locus_tag="KMT45_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBE7005878.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(687..1538)
                     /locus_tag="KMT45_002440"
     CDS             complement(687..1538)
                     /locus_tag="KMT45_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE7005879.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(1555..2385)
                     /locus_tag="KMT45_002441"
     CDS             complement(1555..2385)
                     /locus_tag="KMT45_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBE7005880.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(2388..3074)
                     /locus_tag="KMT45_002442"
     CDS             complement(2388..3074)
                     /locus_tag="KMT45_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005881.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(3080..3319)
                     /locus_tag="KMT45_002443"
     CDS             complement(3080..3319)
                     /locus_tag="KMT45_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005882.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            complement(3645..3833)
                     /locus_tag="KMT45_002444"
     CDS             complement(3645..3833)
                     /locus_tag="KMT45_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005883.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            complement(4139..4327)
                     /locus_tag="KMT45_002445"
     CDS             complement(4139..4327)
                     /locus_tag="KMT45_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005884.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            complement(4330..5106)
                     /locus_tag="KMT45_002446"
     CDS             complement(4330..5106)
                     /locus_tag="KMT45_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBE7005885.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            complement(5233..5454)
                     /locus_tag="KMT45_002447"
     CDS             complement(5233..5454)
                     /locus_tag="KMT45_002447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005886.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            5755..6099
                     /locus_tag="KMT45_002448"
     CDS             5755..6099
                     /locus_tag="KMT45_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005887.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            6135..6530
                     /locus_tag="KMT45_002449"
     CDS             6135..6530
                     /locus_tag="KMT45_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBE7005888.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            6583..6894
                     /locus_tag="KMT45_002450"
     CDS             6583..6894
                     /locus_tag="KMT45_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005889.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            6906..>7230
                     /locus_tag="KMT45_002451"
     CDS             6906..>7230
                     /locus_tag="KMT45_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBE7005890.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIK"
BASE COUNT         2071 a         1378 c         1167 g         2614 t
ORIGIN      
        1 attgcaattt ttctcggcgt tttccgttta ggcttttcag tccacattga tttgccgatc
       61 ctttggactt ctgcaactat ttctttgtct tcctgccaag attctgactt ggttaattca
      121 gcaattcgtt tcattgctgc tttcttatcc acgctcattc ctccaatcta cgaatttccc
      181 ttcttaaatt ctcaatgtgc aaatcaattg ccttccttgc cgtttcattg accatcactg
      241 cctttgtccg ttccagatcg tcaatctcac gctgaaggct tcgaatacgc atttgaatca
      301 cttcttctgt tgtcatgata gtccacctct ttaaaaacgc tcttccttga acgtattccg
      361 atattttttg gctaatatca acggcacttg atattgatga caaaacagct ttgccttgat
      421 cttaaagtct tttgtctgca ttcctttgac atctacgact ttgactagtt tgccgttttt
      481 ataaaatgtg aagtcaggaa tatactcgat cttgcgatac ttctttcctt ctagttcaaa
      541 ttttggcatc agctcaaatc tttcctgaag ttttactttc cagccgttcg cttcagcttg
      601 ccataaggct agatcgtagt actctgcttc tgcgatagaa tcgaacttga tacctcgatg
      661 aacagttttt ttattacggt atttattcat gcgatactac ctttcactgg ttttatgcgc
      721 ttgtctgctg tttgttggaa tttcagcgca taaccttctg aattcttaaa tatcctagaa
      781 acaattcttt cgccgtaggc ttctcttagt tcaggaccag ataagtttgt tgtgatgatc
      841 gttgccttgt tctgtctggc ttctaagagc gtgtttaacg tgttgtttgt aaactgccta
      901 ctatttgata ccccactacc taattcagct ccaatatcgt caaaaaccac caaatcagtt
      961 gttttaatat cggctataag cgatccttca atttcttttc tcagttcagt attgttataa
     1021 gaaaacttta tttgctctaa taattcttga tagcttataa aaagtatttt cttgtcataa
     1081 tttgagcgct caagtatttc ccaagctgtg gccattgata agtggctttt tccgcttcct
     1141 gatttccctg atagaatgaa atgtgcagga tggttcagca gaacctcatt cgtatagctt
     1201 tttgcttttt ctaaagcgat ttttgtttct tgatccacta cgtgataatt ttccattttg
     1261 catttaaaca aagttttatc tgttaatacc gaaccatttt gaaaaaaact caacgctcgt
     1321 gcttttaagc tatcgttata tatccgttcg gtctgtatat cctctttcac acgtaacgct
     1381 ttataaccac aactcatgca tgttggttta caacgttctg aaccatcctt atttttagct
     1441 cgccaactat acaaaggttc gctacattct ggacattttc cgctttgcac taatactctt
     1501 cttattagtt tctccatagc atttgctagg ctttccatgt gatgcatctc ctttttaaat
     1561 tggcaagtcg tcatattcac taggattgct gtactgtagt ttttgacttt gctttttatg
     1621 attcttcttg tctgctttga tttcgaattt gagcttctca aatttttctc tcaatttctt
     1681 agcacttcta atatttccaa accaaaattc atttgtaggt agccaattga tcacatactc
     1741 aatcgcttct atagatgctt tatctctttc ttccatcaac ctgattgtgt ctgcccattt
     1801 ttcgatatct actttgttca tttcttttgg aaaatcttca gttaaattac tttgcaactt
     1861 tttagcaagg cgtaagtgtt cgtcagaata cttacctttc ttttcttctt tatctatatc
     1921 tttatcttct tctatatctt tatctgtacc gtcacgtgac gtcacgctaa cgtcattttc
     1981 caatttgaga cgttcctgtc tctttctttc cctgtattta cggtttcttt cagcattttt
     2041 tagccttact ttatccatac cctcgatatt ttgatgtttt tcccaattac tgatggcaat
     2101 tagtccatca ctgcttagat caatcatgtt gaaatttgcc aatgtagtta gcgctaagcg
     2161 aaccgtattt acgtttttgc caaacaatgt agcaagcatt tcttcggtat aaggcatgtt
     2221 cctctggata tatatcagac catcgtcgtt agtctttcct gctaaaacta gtaatcgaat
     2281 ccatataacg atgatggcat ccgactcagg aacagcttgg attaatcgta ttttttcatc
     2341 gtcaaacata gtagttttaa gtttgatcca acttatctca gccaaattta tcctcctatc
     2401 cttaactttt taattgtttc ctggtttaac ttgatccctt tgatttgata tttatttttg
     2461 aaattgatca cacctatttt gtgtttctct gtgtgatgga ttctgcagag tgctgcaaat
     2521 gtgtactctg aatgatcaac ttctttgcgc tttcgtcttc ccagcgcttt gtcaaagtga
     2581 tcgatatcag ctcctgtttt gccacagatg cagcagactc tttttgtaat gcatttgtag
     2641 aagtaatact cttgattcgc tggtaaaatc tcatatcctt ctttgaaagg aatatgatgt
     2701 tcaaagatga aatctaggat gatattcgct aagatattgg catcactcac ggttgtattc
     2761 gattcgtctt tgaggcttat ttcgcgccct gtgacacctt caaaacggaa gtagaagaat
     2821 tccttccaga agtccgttgg catgcctgta tcgatgaaaa tatcgcctat gagcgcatag
     2881 atgaagtttc tttgctgtac tgtgaaccgt cgtggatcaa taaagcgaat ttcaatgact
     2941 cgatcgccat catatccgtc atacatcgtt ttcagccgtt cgatatttac ttcttcgttg
     3001 attgttgcac caatgtcgtt acctttaaat ttctttagta ccgccgagta tgaatctatt
     3061 agtggtttaa acactcatat cacttctctt ttgtttcttc tctgtactga tcttcaagcc
     3121 aattaacgcc tcgttttaga atgcccaagt ctctcttggt ccatttactg tcatcagcgg
     3181 ttatggaagc tgcatcagtc aatgcaacaa ttgcttcatc aattgatttt tcgtacttgt
     3241 tagcaaccag ttgtaaattc gatttgcccc aatcaggatc ggccaaatat tgatctagag
     3301 tggaaagttc attttccata tgttaaaatc tccttagtta tgattttttg agtgactcat
     3361 tgctttggtc ggcggagtca cttttttatt tgttgccatg cttttttctt ttcgatatgt
     3421 tgcttgctta aaataataga acggctattt gcccaccaat tatcagcaat cactttaccg
     3481 atttttagcg cttcttctcg tgccatagtt gctcctttct tttgagtcaa gcagattgat
     3541 taaaaccatc aatgctgcga acaagcttcc tccgataata ctttggtgcg ctacaatcac
     3601 taataacccc aaaatgaatc ctataaaaag tgtgtctgtc ttcttcataa tctaatctcc
     3661 ctatttttta tttctagcat tctcaaatcc tcaagttcag aagcaattag ttcagcttgt
     3721 ctatctgata gctcatcgga ttttctaagc gctgcacgat catcttgtaa ttgttttcta
     3781 cgctgtttaa tcagacctag aatttgatgt tcttgttgca atgtgtagga cataaaatca
     3841 ttctcctttg cctttagaac tcaaagtttt ctttcaaaaa tctttggagt tccgatcgtt
     3901 caattctgat gtctaacttg ctccactgct gtgtttttaa gcctaggctt atccaatgtg
     3961 ttaatttgtc atcaccaatg cctaaaactt ttttacctct gatttgtttg gatatggagg
     4021 aagctctact gatttgttca taaggtgtaa tcgttcgtcc aatgaattaa gaacagcatt
     4081 cgtaatctgt gtagttaatt cggaaactac taaattatct ggaattgtta tttgcatgtt
     4141 atttctcctt ctctaactct gctagtgctg cctcaattgg cttgatttgt ttatctggtt
     4201 ttctacgccc attcataatg tccgacatat atggtctaga aattccgata gtatcagcta
     4261 accagttctg agactttccg tattttgcta attgaattct gactttcaaa ataaattctt
     4321 gtgacatcgt cattcctcct ctaataaatc gatttctgga atatatcctt cttttttcag
     4381 cgactcataa atgaacagac gccctttttg agtccatttt gtattcatca caacttttgt
     4441 tccgccatca gatttcggaa tctcagttgt atgagatttt gtgtaaccct gtctcatgtg
     4501 ttttttgcat aataaccatt ggttgcctac ttttttctga atacctagct tatgaagtag
     4561 tttgttcatc tgttgtggag acatcccata atcagccgcg atttgactaa tagttactga
     4621 atctgtagaa gataagatac tatccaaata cgaaattttg ggttcgtatt ctgcaatttt
     4681 ttgttctgcg attagtcttc cagtacgttc ttctttcaac ttagttgcta attggatgat
     4741 tgtatctgga ttaagcaaag cttcttctac tttttctgga gttagataac ctccatgttt
     4801 tctaattgct ggcaacactt cgctagtcac ccaacgttta aatttcttag catttggttg
     4861 tttgcttcct aaaattaagg agtacaaacc tgattcgtta ataatagtta accctctagg
     4921 cgattcaaaa gtaccgtttt ggtagttttg ccgatcttct acatcgacgt gtctgttaat
     4981 atctctacta ccattttggt accctagaac tttagccaca tcttttccaa caaaaaatgg
     5041 ttcatcatgt attgttactg ttcttacttc atgttgttca aagttaaaaa tttgcggtgt
     5101 gttcattttt gctcattcct ttctttgata taattttgaa tagaaagcga ggtgaaaata
     5161 gtatggaaga atttaatatg gatgtcgacg ccttattcaa acaaaccgta ttcaagactg
     5221 ttaataaaga attcaaaata gacttccaaa atgacgaatc ttttccaaca gaaattgaat
     5281 tattcgaaga actttctcag agcgtttctg agtctttttc tcgtcagttg caaaagaatt
     5341 tttttgatgc tcttgtggat gaatttcatc aacaacagca ctaaactttt ggtctttaaa
     5401 atttaaagtt attaaagctc cttttgcgga aggagttttt ttattttgtt tcatgatgtt
     5461 tcctcctttt ctttaaatat gtaagctaat aaaattagct aatttttatt gacatttcta
     5521 tccaactgtc ttatatttat atacagttaa ataagcctag aaaaacccta aaaattaaca
     5581 ttcttagttt gccgacctcg aattcgttta ttttattagg tgtatttctt gttgcttgtt
     5641 agcttattaa attagcttac gagttaaata taagacaatt ggatagattt gtcaactacc
     5701 tttctatctt tttgtataaa ttattatcgt acgctctagg aaggttgatt tgacatgctt
     5761 ttcgaaaggg ttaaagagct ctgtaaaaaa agaggtattt ctatttctga attagaaaat
     5821 agaataggat ttggaaaaaa ctctatttat aaatggaaga cacaaagtcc taaaatagaa
     5881 actttgcaaa aagttgctga ctacttccat gtatcaacag attatttgct aggacgcaca
     5941 gatgatccta acgcgggagt tgcaccagag gaaagaaaac taaccgtgga agaagcttta
     6001 gcatctgtta tgagtagcga cggaaaaccg ctcaccgata atgataggga aatactatca
     6061 gctatgattg aagcatattt agagaaaaaa gataactaaa taagtaggtg agtcatttgg
     6121 acagtcaaat tgaaatgata attaatgaac tcggcgttaa ggtagaagag cgtgaaaacc
     6181 ttgatgccga tggccattat gttgcttgta tgaataccat agtaataaaa gctaatttat
     6241 ctaagtatag aagacaaaga accttattac atgaattagg acacgcttct aaacatcatg
     6301 ataattattt tttatataac ttagcattct ctctccattc aaaaatggaa tatgaggctg
     6361 atcgcttcat gattgaaaaa ttattagata gatatattgc aaagtctgaa ttagaaccac
     6421 acaatatcaa ttacatgaaa tttatagaag ataacaattt aagcgttcgc ttcgaaccac
     6481 ttgtgaaaga attattaaaa gctcgcatct attgttatgc agctctctaa aattttttaa
     6541 gcaaaaaaga acatacgttc aaaaatagaa aggtgaacaa aaatgatata tacagaattc
     6601 aaagaatggt tagaaaaaag cacaaccgga tacgaaacat ttatcatcaa agctactaat
     6661 tatcaaattg aaaaaaacaa aaatagaccc caaaaaaaac gctgggatga taagaaaata
     6721 gataaagctg tattagaaat gtggaaacaa gtcgtgacta acttgtatca aacaattcgt
     6781 aaagaaaaag gagttccatt aattaacggg aaggaaatat ggcttgaatt tatagaggaa
     6841 caaggactga tcgaattttt caatgatagc atggcagaat tagaatttga ataggggtaa
     6901 tattgatggc aatgataaaa caatataaaa agaaaaatgg cgaaaaagca tggtacttta
     6961 aaacttatct cggtattgat ccgctaactg gaaagaaaaa atatactact aaacgaggat
     7021 ttagaacaca aaaagaagca aaaacagcac tttctaggtt agaactagaa ttacaaaaaa
     7081 caggaatgcc cacaagtaca aatactactt tcaaagaagc agcagaatta tggctagaaa
     7141 gctacaaaaa aactgtaaaa gaaagttcat attcaaggac taaaataatc tttaataaac
     7201 atatatatcc caaatttgga aatattaagc
//
LOCUS       DAEGIQ010000124         6948 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000124 DAEGIQ010000000
VERSION     DAEGIQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.128"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            124..597
                     /locus_tag="KMT45_002452"
     CDS             124..597
                     /locus_tag="KMT45_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBE7005891.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            594..2726
                     /gene="feoB"
                     /locus_tag="KMT45_002453"
     CDS             594..2726
                     /gene="feoB"
                     /locus_tag="KMT45_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBE7005892.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2728..2862
                     /locus_tag="KMT45_002454"
     CDS             2728..2862
                     /locus_tag="KMT45_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBE7005893.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            2956..3600
                     /locus_tag="KMT45_002455"
     CDS             2956..3600
                     /locus_tag="KMT45_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7005894.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3787..4980
                     /locus_tag="KMT45_002456"
     CDS             3787..4980
                     /locus_tag="KMT45_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBE7005895.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            4973..6700
                     /locus_tag="KMT45_002457"
     CDS             4973..6700
                     /locus_tag="KMT45_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBE7005896.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2238 a         1224 c         1557 g         1929 t
ORIGIN      
        1 tctcaattac cgagagtatt ctatgtgaga atgattctca tttatttgcg taacacgtaa
       61 gaaaattaga acatataatg aatgatttta atacatgatt tatttcacaa gagaggaatc
      121 aatatgcaga ccttagcgca ggcaaagcag aatttcgttt atcaagtcga acgaatcgaa
      181 acagaagaaa aaacaaaacg ccatttgcaa aatatcggga tcgttgccgg aagtaaaatc
      241 gtcgtcgtca atttatcagg caacaatgcc attttactgg taaaaagtca tcgagtggca
      301 gtaagtcggg aaatactagg tcaaatcttt gtcaataaaa agactgcttc ctctgaaagc
      361 tggacttctc ttgatcaatt aaaagcagga gagactggga tcgtcgtagc aatccacgga
      421 caaggcgcag tgaagcgacg gttaatggat atgggattga cgcgaaatgt aaaaatagtt
      481 atccgaaaac tagcccctct gggagatcca atcgaactga cagtgcgagg gtatgaactg
      541 acgttacgaa agtcagaggc cgaactaatc ttgatacaaa gggaggaaaa agaatgaata
      601 tcgcgttagt cggaaatcca aacagcggga aaactagctt gttcaatctc ttgactggtt
      661 cgaatcaata tgtagggaat tggccgggcg taacagtaga aaaaaaagaa ggatcgttga
      721 aaaaaaatcc gaacattcat attcaggacc tacccggcat ctattctttg tctccttata
      781 caccagaaga agtgattgca agagattatt tggttcagga acagcctgaa ctgatcatca
      841 acattattga tgctaccaat ttagaacgaa atctttattt aactacgcag ttattggatt
      901 tagacattcc tatgatcatt ggtctgaata tgatggatgt catcaaaaaa gaaggaaaaa
      961 aaatcaatct agaaaagctt tcctatggtc ttggtgtacc agttttacca atcagcgtat
     1021 tgaaaaagca aggaatcgaa aaactaatcg aaaagattca ggagtctgca aaaaagcggc
     1081 cttctattcc tgttccctgt acatatgatc cacgtttaga agcagcttta catgaaattg
     1141 aagatgtact aggagaacaa agcggcaaga aacgctggta tgcaatgaaa tattttgaac
     1201 gagatcagaa ggtaagagag gattacgaga tagcagtttc tcagcaaaaa gagatcgagc
     1261 aactgatcca gttaacagag aaactattgg acgacgatag tgagacgatt ctggtcaatg
     1321 aacgttatga atttattacc caactatgta cactctgtgt tgtgtcaaat gattctttcc
     1381 aattatcgat gagtgacaaa atcgatcaga ttgcaacgaa tcgttggtta gccttgccga
     1441 tcttcgcatt tgtgatgtgg ctgatctatt atctggctat tcaaacagta ggaacaatgg
     1501 ggacagattg gatcaatgac acattgtttg gtacatggtt accagaacat gttggcagat
     1561 tgttagctga atggcaagta gcgggatgga tgcaggagct gatcttgaac ggcatcatcg
     1621 caggcgtggg agcagtgtta ggatttctac cgcaattgat tatattgttt ttatgcttga
     1681 gctttctgga agactgcgga tatatggcga ggatcgcttt tgtcatggat cgacttttca
     1741 ggaaattcgg tctttcagga aaatccttca ttcctatgct gattgctaca ggatgtggag
     1801 tgccaggcgt catggccagt cgcacgattg aaaacgagca agatcgtcgt ttgacgatca
     1861 tggtcacaac cttcatgcct tgttctgcaa agttgccgat tattgcctta gtagcagggg
     1921 ccttttttcc tcatcaatct tgggtagctc cttctgccta ttttcttggt atgactgcaa
     1981 ttattttctc tggaatcagc ttgaaaaaaa cacgtttgtt tgctggagat acagctcctt
     2041 ttatcatgga attgcccgca tatcattttc ctcaatggtc agcggtccta agacaaacct
     2101 atgaccgcag caagtctttt gttaaaaaag caggaacgat tatctttgtt tctagtatcg
     2161 tgatctggtt cagctcttct tataattttt atgctcaacc tgtttctgaa gaccaaagta
     2221 tcttagcctt tttcgggcga atcctggcag ttgtttttca gccgcttggt tggggaaact
     2281 ggcaaggaac agtcgcagcg attacaggat tggtcgctaa agaaaatatt attggcacat
     2341 ttggtatttt gtttggtcat gcggaagtat ccgaaaatgg cagagaaatc tggcaagtgc
     2401 tacaacacac atatactccc gcagcagctt attcattgtt agccttcaat ttactttgtg
     2461 cgccttgttt tgcggcaatt ggggctatcc atcgggaaat gaatgccaag aaatggactt
     2521 ggatcgccat aggctatcaa tgcggtttag cttatttagt tagttttgtg atttatcagt
     2581 tgggacactt attggagggt ggacaggtag cgttagggac gtatcttgct attttactga
     2641 tgataggact cgtttatgtt ttgttccgtc agccaaaatc caagaaccag ttttatacta
     2701 tattgtcatt agaaggagag gaataggatg gcgacattct tattaagtgt gttaatcttt
     2761 ggaagtgcag gcgcaattgt ctatactcgt ttaaaatccg gtaagaattg tgatgactgt
     2821 caaacagctt gcccagtaaa aaaggaacag atcaagcgat gatcagtcgt tgcaagaggc
     2881 tattctcccc ttttttgctt attttgctag agcatagtaa gatgagggaa agtaaaaaaa
     2941 gaaagaggaa agaaaatgtc tggaaagaaa aaaatcgtcc gcccgcgcta ccagcagatt
     3001 gctgttgatt tagccgaacg tatcgttgaa aaccgatata aagtgggtga gaagatccat
     3061 gcccgctcaa cacttgccag tacgtacaat gcttctcctg agactacccg caaggctatc
     3121 aatgtgctgg tagatcttgg aatcatggaa gtacggcatg gaagtggggc atttgttgct
     3181 tcgaaggaaa aagcaaaaga tttcctattg caatatcaag atgtacaatc catccaagat
     3241 acaaaagcag aaatcatggc aagtgtggag aagcaacaag atgagctgaa tcatttttct
     3301 caattattgg atcagctggt caatcaaacg aagcgaatac ataagatgaa tcctttactt
     3361 ccgtttgaat tatctttatc agaaaaagca atgcatctgg accgttcaat tagcgagttg
     3421 aatatctggc aggcgactgg cgccacagtt atcgcaattt ctcataatga agaactgctt
     3481 gtttcacctg gcccctatgc taaatttacg gcaggggaca tcgttcattt tattggaaat
     3541 gaatatacac tacagcgtat gacaaacttt ttttacccgg agcatcagct tgcagaatga
     3601 ttgtgtgaaa tattaaaaaa atattaactt ggctaaacct tttatttaca aagatttagc
     3661 caagtttttt tcagtttgga caagcaaatt tctttcattt ttgacaaagt gactacatga
     3721 tgttaacata gtgtggtcac ttttttcaga aagaaaaatg aatttacata aaaaaggaga
     3781 atttgtttgg taaaagtaga agtaaaacat ttaacaaaga tatttggtaa aaagacacaa
     3841 gccgctttag atatgatgaa tgatcaccag ccgaaaacgg agattttaaa gaaaaccggt
     3901 gcaacggttg gtgtgtatga cgtgaatttt gacgtaaaag aaggagaaat cttcgtgatc
     3961 atggggcttt ccgggagtgg gaaatccaca ttgatccgtc tattgaatcg tctgattgag
     4021 ccaacttcgg gcagtatcta tattgatgga gaagatgtat cgaagttaag caaagaagaa
     4081 ttgcgagaag tccgtcgtca taaaatcaat atggttttcc aaaacttcgg tctgtttcca
     4141 catcgaacga tcttggaaaa tacagaatac gggttagaag tccgtggtgt acctaaagaa
     4201 gaacgacaag aaaaagcaga aaaagcatta gaaaattcta gtttgctttc atttaaagac
     4261 caatatccta gtcagttatc tggtgggatg caacaacggg ttggcctagc cagagcttta
     4321 gcaaatgatc ctgagatctt gctgatggac gaagcgttct cggctcttga tccgctgatc
     4381 cggcgagaaa tgcaagacga attactggac ctgcaagcca atgttcaaaa aacgattatt
     4441 ttcattaccc atgatttgaa tgaagcttta cggattgggg acagaatcgc cttgatgaaa
     4501 gacggcgaaa tcatgcagat cggaacaggt gaagaaatat tgaccaatcc agcaaatgat
     4561 tatgtacgtg aatttgtgga agaagtcgat cgttccaaag tgcttacggc tcaaaacatc
     4621 atggtacctg cattgacgac gaatatcgaa tctgatggac caaacgttgc attgacaagg
     4681 atgcgtaatg aagaagtcag catgctgatg gctgttgaca gaaagagaca cctgaaaggg
     4741 atcatcactg cagatcaagc gttagaagct aggaagcaaa aacgtccatt gatcgacttc
     4801 ctagatgaaa atgtgacggt gattgggaaa gacatggtgg taagtgatat tttcaatatt
     4861 atttatgact caccgactcc acttgcagtt gtagaggatg gaaaattaaa aggtgtcgtc
     4921 atccgaggta gtgtgattga agcattagca gaaacaagcg aggtgagtga acatgaataa
     4981 tctattgaac tatcaattac cagtggcaaa ctgggtagaa aatatcacag aatggtttac
     5041 gacaactttt tctggcctat tttcattttt acaaacgatt ggacaggcag tgatgtcggg
     5101 gattacaaat ttactgttag tgattccagc tcctttgttt atcttgttat tgacggtagc
     5161 agctttcttt atttctaaga aacgtccagg acttacgttg ttcacattaa tcggtctatg
     5221 gtttatttat aaccaaggtc tatggaatga tttgatgaat actgtcacac ttgttctttt
     5281 atctagtgtg atctcgatca tcattggggt accattaggg atcttgatgg caaaaagcag
     5341 taaagcccaa agtattatca aaccgatcct tgactttatg cagacaatgc caggattcgt
     5401 ttatttgatt ccagcagttg catttttcgg tatcgggatg gttccagggg tatttgcctc
     5461 agtcatcttt gctctgccac caaccgttcg ttttacgaat ttagggattc gacaagttcc
     5521 taaagaatta gtagaagctt ctgattcatt cgggagcaca agtcgtcaaa aactattcaa
     5581 actagaattg cctttagcta aaagtacgat catggcaggg atcaatcaaa caacaatgct
     5641 gtcactttca atggtcgtta ttgcctctat gatcggtgca cccggcttag gacgcggcgt
     5701 attatcagca ttgcaaagag cacaagtcgg gaatggattt gtcaacgggg ttgcattagt
     5761 tatcttagca atcatcattg accgttttac acagcatttg aaccagccaa acaataaaaa
     5821 agctgcagga gcaacaacac agaaaagtaa aaaacgtcaa ggactgatca ttggtgctgt
     5881 agtagtagtg atcttaggtg cgattggtat agggagtttt tcatctgcca aagaaacaaa
     5941 acgtatcaac ttatcttatg ttgaatggga tacagaagtt gcttcaacaa atgtagtcgg
     6001 tgaagtattg aaacaaatgg gctatgatgt aacgatgacg ccattagata attcaatcat
     6061 gtggaaatct gtttcaaatg gcgagtcaga tgccatggtt tcagcttggc tacctaaaac
     6121 tcatggttca caatatgcac aatataaaga tcaagtcgaa gacctaggtg ccaatttgac
     6181 aggagcaaaa gttggtttag cagtgccagc ctacatggat gtaaattcga tcgatgagtt
     6241 aaccgatcag gcaggtaaga aaatcatagg aatcgagcca ggagctggtg tagttacggc
     6301 tgctgaaaac acgatccaaa aatatgacaa cttgaaagac tggaaagtag agacatcttc
     6361 ttcaggagca atgaccgttg cactaggtca agcgatcaaa aaacacgaac caattgtagt
     6421 tacaggatgg acacctcatt ggatgtttgc caaatatgat ctgaaatact tagaagatcc
     6481 tgaaaacgga atgggaagcg aagagcagat ccatacgatg gttcgaaaag gattgaaaga
     6541 agaccagcca gaagcttaca aagtattaga caatttccat tggtcagaaa aagatatgga
     6601 aaaagttatg ttggaaatca acaatggaaa agatccacag caagcagcaa aagactggat
     6661 caaagagaac caagaattag tcgaaagctg gaaaaaataa aaagacagac acgaaataaa
     6721 tggagcttcc aaaattggat caaatatcca aatttggaag ctccatgttc gtctctatct
     6781 tttttgtcta taaaaaaaga gaatgcgtag ctcatcttcg aaacgttcaa agacgccgac
     6841 aaactattat gaatttatgg gtgcgagtca aagagatcga tcgtaggtat gaaagactaa
     6901 tgtgattaaa gtttattatg tacgaagtgc attctcaaaa aatattaa
//
LOCUS       DAEGIQ010000125         6946 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000125 DAEGIQ010000000
VERSION     DAEGIQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.129"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            43..801
                     /locus_tag="KMT45_002458"
     CDS             43..801
                     /locus_tag="KMT45_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBE7005897.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            861..1289
                     /locus_tag="KMT45_002459"
     CDS             861..1289
                     /locus_tag="KMT45_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005898.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            1335..1407
                     /locus_tag="KMT45_002460"
     tRNA            1335..1407
                     /locus_tag="KMT45_002460"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1368..1370,aa:Gln,seq:ctg)
     gene            complement(1557..1928)
                     /locus_tag="KMT45_002461"
     CDS             complement(1557..1928)
                     /locus_tag="KMT45_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBE7005899.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            2143..2328
                     /locus_tag="KMT45_002462"
     CDS             2143..2328
                     /locus_tag="KMT45_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005900.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(2411..2599)
                     /locus_tag="KMT45_002463"
     CDS             complement(2411..2599)
                     /locus_tag="KMT45_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005901.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2623..2886)
                     /locus_tag="KMT45_002464"
     CDS             complement(2623..2886)
                     /locus_tag="KMT45_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005902.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            3044..3301
                     /locus_tag="KMT45_002465"
     CDS             3044..3301
                     /locus_tag="KMT45_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBE7005903.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(3372..3563)
                     /locus_tag="KMT45_002466"
     CDS             complement(3372..3563)
                     /locus_tag="KMT45_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005904.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(3704..4024)
                     /locus_tag="KMT45_002467"
     CDS             complement(3704..4024)
                     /locus_tag="KMT45_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005905.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(4047..4481)
                     /locus_tag="KMT45_002468"
     CDS             complement(4047..4481)
                     /locus_tag="KMT45_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBE7005906.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            4816..5454
                     /locus_tag="KMT45_002469"
     CDS             4816..5454
                     /locus_tag="KMT45_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBE7005907.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(5778..5861)
                     /locus_tag="KMT45_002470"
     tRNA            complement(5778..5861)
                     /locus_tag="KMT45_002470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5825..5827),aa:Leu,seq:cag)
     gene            6389..6889
                     /locus_tag="KMT45_002471"
     CDS             6389..6889
                     /locus_tag="KMT45_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE7005908.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT         2127 a         1345 c         1115 g         2359 t
ORIGIN      
        1 tttataatag gaaagataaa cagctgaaaa gggagatttc aaatgaagaa aaaaacgttc
       61 aaaaaactat tgtttctagg gggcacagct gctcttttat taagcggtac aagttattac
      121 ctacatatcc attctccaga acatacaatg aacactgcac aaaaaagttc aacacaaaaa
      181 acgaaaaaga caaaagagaa agcagaagtt ttactagatg ttccattaga gagccagttt
      241 gattctcctt ctttagaaaa tggctgtgaa gtcacttcgc tttctatgct tttatctttt
      301 tatggttatg aaacaactaa aaaccagtta gctgagcaat tggattacgt ccctgttttc
      361 aatgcagatg ggactcatgg cgatcctaat gaaggatttg ttggtgatat cagtggcgga
      421 gactgggcta tggtcgtcta tgttccccca gttgcctccc ttgctcagca aatcgtccaa
      481 acggattatc acacttttcc aaaaaccgat gctcaactcg atgatatcaa aaaagcacta
      541 tctaaaggaa atcctgtctg gacttctgtg actatcaatt ttgaagtccc agatgaaact
      601 gattttatga cttggaccac aaataatgga gaagtaagag tcactcctct tgttcatgct
      661 tgtgtagtca ctggatatga caaagaaaat atttatgtca acgatcccta tggtgtaaaa
      721 aatcgagcag taccgatcaa cgatttttcg gcgattttca ctgcaatggg tggacagatg
      781 ctaactttgg agaaaagcta gtaattcaaa caaaaatccc ttatactcaa gataccaaac
      841 ctttgaaggg agcgtagata atggaaaaat tcacacataa aaaaatggat ccaaatgaaa
      901 ttccgattat ttttgtccgt gactgcaagg gaaatgttca aggtaaagtc tctattaatg
      961 agtggaacga gcgtcgcaga cctgctaccc tcaatgaact ggaaatcaaa ttgtatcgtc
     1021 aatctcttgt ttattacgca gatcaagagt acgagaaagc caccgattta ttgaaatttc
     1081 taattgctcg aacagagtat actcgctttg aatatattga acgtttagca aacatctatc
     1141 atattatgaa tgaacctgtc aaagaatatc agttattgga caccgtcttg tcagtagcag
     1201 aactgattgc ccttcctgct ggattagaaa agaaactagt acgccgcctt ttacgagtaa
     1261 agcaacaact atccgatcaa gaaaaataac ttgcgttttt ttaggaaagt gctatgatta
     1321 ttttgttgaa taaatgggat gtagccaaac tggtaaggca cttgactctg aatcaagcaa
     1381 ttgtaggttc gaaccctgcc atcccaattt gatgtgcttg ctgaaaaaat gaaccccctg
     1441 taaaagaaca atctttttgc agggggatta ttgtattatt tttactacta gaatttagtt
     1501 tacataatta aattatgaat aataaaatcg ggcgtattct taatacaaaa actgatttac
     1561 ccaataattg ctaatgcttt ctccggaact ttttctctag gaacttcctc atacgtttct
     1621 atgtgtgctc ctttattgct aattttcaaa taatgatttt cttttaacac agaaagacca
     1681 ttaaattcaa ccgttcgttc tttcccattg gcatcagcag ctttctgcac ataccttgct
     1741 gtaccatatt catttacttc ctctggtttt tgtgtcttta catagacctc tcctttttca
     1801 actaatggat ttagctgatc aagtatacca gggatttctc cataacttcc ttcagtataa
     1861 tatttcagtc caaatagcaa aataccacta atcgccgcca gtccaatcaa atatttaatc
     1921 actttcatct ttctacctcc ttgttatata ttcagtatat gggtcagtta aagaaattcc
     1981 atccgtccca agttggagat ttttctccta ctttagaagc gagaatcgag tgttttttta
     2041 gtaaaaatga tcgttatttg atattattga cgtgtggata aatcagtttt tctcgttttg
     2101 tccatgtaaa aaatcatgtt gatagcaaag gatgtgttca aaatgaatca agaagaatta
     2161 tttcaaaaag tcaaggaaat gattaagaac ggcaatttcg atggcgcaaa acgatttatc
     2221 gaagaacata aagaacaatt aggaccttat aaagagaaag cccaaaatct tttaaaagat
     2281 gtaaatatcg acagtgtcaa aaacaaattt aaaaatctat tcaaataagt aaaactttca
     2341 cttttgtaaa atcaaaggag gacaatgctg ctgcatcgtc ctcctttggt tcttttctga
     2401 atacttcata ctaaaataaa cgtgtcggat cgtaatgata agtcacattg ccgttcagtg
     2461 aggcttttcc caccacatat ttacgattct ccaacttcag ccacgcttct cgaaaagcca
     2521 ttaattcttc tctttttaca tcaatctttt cgatttttcc atcgatcaaa tcttgaataa
     2581 gttgttcata tggattcatt tttttcctcc atctgctgtc aatcaataag ttgtaaaaat
     2641 aggttcatat gcttttttgg tatctagatc ttctctgact ttgactgtcg tataaataat
     2701 tttttctact gggatactat aataacttcc ttcaccatta ttgaatcggt gaacgtcttt
     2761 ctttgctaaa ataccattta ttttattcgt cagtacacgt tgttcgacac tgttacttac
     2821 aacatagtaa atcacccgaa tattattgat caaatgcaaa tcgatttcta acactacatg
     2881 atccatgaaa atcccactcc ttctttcact ttgattatag caagtttctc ctctaatttc
     2941 aaaatcgata cagattttac cggataaatc cacaaaactg cttgcgccat ttcgcacaaa
     3001 gtgttatttt gtatcatgga ctttatttat cggaggcaat agtatgatcg cgatcggtca
     3061 atttgttttt tatattcctt tttttattat gataagcatt cttttctatt acataaaatg
     3121 gacaaaaaag aagttttcag ttttactggc ctctttacct gctgtatatt ttacttatca
     3181 aattttttct tttaggcatt gggaaacaac gtctgtatta ataacacaca tcatcgaatt
     3241 gacgctttca gtaatttttc ttattatttg gatctatttt ttatataaga atcagaattg
     3301 aatccctcgc atgtttaaaa agaaaacgat taaagatgca gcaaatttct gccgatcttt
     3361 aatcgttttt tctattctgt tataccaaaa aaatgtttga atcggtgaag gatcgttcct
     3421 tgttttaatt ctataaattc ttgtgcgatc gggtactttt cttccaaatt ttctttgttc
     3481 ttttcgatca ggcttttcgc ttcttcatat tttttatctg cacatagtgc ttttacttcc
     3541 tgtaatactt ttcctgcttc catttgcctc accctcttgc cattttattg ctttaagttt
     3601 tatttaagtt atacgcatta ttctagtttc atcccaaata accttttttc atttttactc
     3661 ctcgaactaa tcagttttct aaaggctgat tggttcgtct tttttatttt tcctccattt
     3721 cattctgctc ttctgttaat ttatctaatg ctttgattga tgctgcggct cttgcataat
     3781 catagctgta taattccata aatttatcag gcagaacaaa ccacgctgga tgataaccgt
     3841 tagctgtgac agggaatcct ttatcttgtt ggttatctcc atcccatctc agagcaacac
     3901 ctttttttcc tttccacttt actaaagcga tcgagtatcc cttctttgtt tgtgtgccgt
     3961 cttcaataat tgctaaaatt tctatttttt cttttggcgc acggacttct tgaggacgaa
     4021 ccattttcta ttcctccctt atctcattac ttcacgacca acacactgca aggggcatgt
     4081 gatacgacat aagaagcggt cgatccaact aatgcttgtt gtatagcacc ttttcctgtc
     4141 gctcccataa cgatcaaatc aatattttct tgtccaggga tgacattggc aatcaaattt
     4201 cttggatctc cagtttcaac tattgcgtgg atctcagcaa tgccgtactc tttggcatca
     4261 ttgatttttt tcagcatggc tacttctgtt ctttgttttt catctgcata gatttttgaa
     4321 aaggcatagg cactatgggt cagctcaact ttgttgatga ttgacacgat aaataagtgg
     4381 gcttcattgt cctttgctaa gtcaagggct tcagcgaacg ctttgatcga ttgtcttgat
     4441 ccgtcaactg ctaccatgat gttctggtat cttttactca ttggaaccgc ctcctttttc
     4501 ttttattgta acatctcaag caaaaataca aaaaatatac gcaatatgta aataaaatga
     4561 tgaacaaaag caatttcttt caagttattt atacatttct tttctcatca gaacacctgt
     4621 tctaatccca ttatttctgc attatgtgaa attcctcatc atttttcaca tcgagcgaac
     4681 atcattctgt tacaaaaaac gtggatttgt cataaattgt catgcttctt ttacctatga
     4741 aatattttca cgtgttaaag tattcctcgt tgaacacaac aaataaaact ttttaacgaa
     4801 taaaggagat ttttcatgac atcacttaaa actttacttt ttggaacaac tttggctgcc
     4861 ggcgctgcat tcttcatggg aacgactgct catgcggacg aagcttatac cgttcaatca
     4921 ggtgatactc tatcaacgat ttctcaaaaa tacgttggtg ataactcctt gatcaatgcc
     4981 attgcagaat caaattcaat ttctgatatt aacctgatct actcaggaca acaattaaca
     5041 atcccaacag aaggctctgc acaagcagct gctgaacctc aagcagccgt tcaagaagca
     5101 ccagtccaag ctgaacctgt tcaagctgaa caacctgttg tacaagaaac ggttcaaacg
     5161 gaaacacaag cagcgcctgt tgctgaaaca caacctgctc ctgctgtaac agaaactgct
     5221 gctactccag caagtacaag ctctgcaaaa gaatggatcg cacaaaaaga atcaagcggt
     5281 tcttacacag caacgaacgg acgttacatc ggccgttacc aattagattc ttcttactta
     5341 aatggtgact actctgctgc aaaccaagaa agagtagcag aacaatatgt tacttcacgt
     5401 tatggttctt gggaagcggc gaaagcattc tgggaagcaa acggttggta ctaagattca
     5461 ttatttgaac gtttaaacac attcaaaata tagtaagagg tcatgcgatt tattctttcg
     5521 catgacctct ttttgagttt aagtttagtc tttattcctt aactatcgtt tgccagtatt
     5581 tcttgtatca gaatctataa atctactatt tgaatcaatc tattaggttt taaactgtcc
     5641 caccttcaca caaatttttt ttgatttatt tgaatctggt tccgttgttt aaaatttttc
     5701 ctatattctt ttagataata tgttattttc tttctcatcg ctccaggtgt accttctctt
     5761 tataaaaaaa caaattatgc ggctaagaag actcgaactt ccacgaggtc accctcacta
     5821 gcgcctgaag ctagcgcgtc tgccaattcc gccatagccg catcacatct tttagtttag
     5881 gattacatgt gttttttgtc aagaacatat ttttgttctt tttcatttct gtacgccctc
     5941 ccttatgcca tctacacaaa taattcatcc gagcaactcc atcatctatt gagggttggt
     6001 gaaaaaatct gttttatcca tatgatcgat ttcaatgtta aacgaacgac tctgggatta
     6061 gtaactattt tatttttttc ttcatttctt ttttcctctt tttctcgatt cagctatctt
     6121 ctttcatttg gatattgatt atttcttctg ttgattacta tccttctgga attctagacg
     6181 ctgaagtaat tcacgttttc tttcttctag agatcataag taacgaatga acgaacttca
     6241 aataattttt ttattgtaat tcatatttgt attgttcgat gattcttaat acgatatgac
     6301 acaataaata aaaataggca tggctattta caacaatagt gctatagtag aaaacaaatg
     6361 aataaggtta cttaaagagg tgaatacgat gaaactgatc aaactatggg aagcagattt
     6421 aaagaaagct tatcagctac aaactagctt tggaccaaat gagagtggat ttatcaacaa
     6481 tgcttatggc tatacatttg ctgaattttt agattatgta aaaaaatgcg aaaaaacgtc
     6541 tttaggaatc gatcttcctg agagttacgt tcccgcaacc gtctttattt tagaaagtga
     6601 tgaacatgag tatgtaggaa tttttaattt gcgccataaa ctgaacgaaa cattaagaaa
     6661 cggcgctggc catattggtt acggcatctc ccctaattat cgtaaaaaag gatatgccac
     6721 acaaggatta gcattagtac tacaagaggc aaaaaaaatc ggcattgatg aggcactttt
     6781 gtctgtaaat aaaaaaaatc ttggcagtct taaagcacaa caaaaaaatg gggctgcgat
     6841 ttatcgtgaa gatgagaaag aatactatac gaaaatcaaa ctgatgtaaa aaagaaggat
     6901 tccaaaagga aagggatttg aatgtccttt atcaatagaa cacatt
//
LOCUS       DAEGIQ010000126         6715 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000126 DAEGIQ010000000
VERSION     DAEGIQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.130"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            17..457
                     /locus_tag="KMT45_002472"
     CDS             17..457
                     /locus_tag="KMT45_002472"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBE7005909.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            585..1268
                     /locus_tag="KMT45_002473"
     CDS             585..1268
                     /locus_tag="KMT45_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBE7005910.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1577..1650
                     /locus_tag="KMT45_002474"
     tRNA            1577..1650
                     /locus_tag="KMT45_002474"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1611..1613,aa:Arg,seq:tct)
     gene            1809..2168
                     /locus_tag="KMT45_002475"
     CDS             1809..2168
                     /locus_tag="KMT45_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBE7005911.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2291..3922
                     /locus_tag="KMT45_002476"
     CDS             2291..3922
                     /locus_tag="KMT45_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBE7005912.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3984..4625)
                     /locus_tag="KMT45_002477"
     CDS             complement(3984..4625)
                     /locus_tag="KMT45_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBE7005913.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4811..4887
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4907..5215
                     /gene="rplU"
                     /locus_tag="KMT45_002478"
     CDS             4907..5215
                     /gene="rplU"
                     /locus_tag="KMT45_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBE7005914.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5242..5586
                     /locus_tag="KMT45_002479"
     CDS             5242..5586
                     /locus_tag="KMT45_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBE7005915.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5599..5892
                     /gene="rpmA"
                     /locus_tag="KMT45_002480"
     CDS             5599..5892
                     /gene="rpmA"
                     /locus_tag="KMT45_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBE7005916.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(5985..>6715)
                     /locus_tag="KMT45_002481"
     CDS             complement(5985..>6715)
                     /locus_tag="KMT45_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE7005917.1"
                     /translation="NKVFKYAINIGLTSDNPTLNLLIPKPQIKTEKKLKLYTKEQLEL
                     FLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEILALTWDNINFKTNEMAIKKTVARS
                     DKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLEQRKYLFQLGFTKANYLFTNDENNF
                     TINQSVAERYNIYRERAGLPYIGLHGFRHTHASMLYEAGADHKEVQERMGHANIKTTM
                     DTYTHITNSKKEETTQKLTNYINF"
BASE COUNT         2047 a         1113 c         1398 g         2157 t
ORIGIN      
        1 tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata ttattgagga
       61 caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac cgaaaaatac
      121 aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa aaaaaatctc
      181 gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa aagctgatac
      241 aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta gtcccatctt
      301 tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga ttcaggggtt
      361 attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct ttgtagagtt
      421 gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat
      481 tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat gcagcttgta
      541 gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc aatgaaatga
      601 tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag caaaaaaaat
      661 taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta ccagttattc
      721 cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct gaacgaatct
      781 tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca atcggagaaa
      841 acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact
      901 atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca gcagatatct
      961 tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa tcaaaatata
     1021 ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg atggtagatg
     1081 acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt ggcaaacgag
     1141 ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc gatttgacat
     1201 cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca gaaacaatcg
     1261 agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg gctcaattcg
     1321 aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga aatctaaata
     1381 aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct tagaacagct
     1441 gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat aattcattat
     1501 ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta taattattga
     1561 tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct tctaagcgga
     1621 cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta
     1681 acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa
     1741 aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc agaaaagagg
     1801 aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt gaattttttt
     1861 atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg gtttttaaaa
     1921 tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac caaggagtgt
     1981 acaatggact attaggagtg ggactacttt atggagctta tctttcttcc gcatctaaag
     2041 aaatcacaag tatgctatta atatctattt tttttgttgc attatatggc agtttgacta
     2101 gcgatcgttg gattttactc aaacaaggtg ggctagctct gttggcattg ctttctattt
     2161 tcttataaat atagaaaaaa taagaattta tctaacagga aactttgtga aaaatattac
     2221 tttatctcat gaaatatgat attctaatga atggtatttt agaaaatttc atgaaaaagg
     2281 agttagatag gtgttcaaaa attatgtttc ctcactaaaa aaagaatttt caggttatga
     2341 tctttcaaga tttcagaaag atcttttggc tgggatcaca gtagctgctg tagcattgcc
     2401 tttagccttg gcttttgggg tatcaagcgg agctgatgcg gccgcaggtt tgattacggc
     2461 tatcattgcc ggtatcgtta ttggcggttt atcaggcggt ttttatcaaa tatcaggtcc
     2521 gacaggtgcg atggcagcta tcttgatgtc tattgcggca acaaagggaa tgcaaggtgt
     2581 attgcttgct acttttttag cgggtctatt tttacttgca gcaggacttt tccgattagg
     2641 aacactgact tcttttatcc cagcacccgt aattacgggg tttacttcag gtattgctat
     2701 cattattgca ttaggtcaga tcgataattt atttggtgtt cactcggaag gtacaaacgt
     2761 tattgaaaaa ctaagtagtt atcaaacgat aggatttccg ataaacacag cctctttgat
     2821 catgggaggg ctagtcattt tagggatgtt tatctatcca aaaaaatggg cgaaacgaat
     2881 gcctagttcg ctgctggcta tcattttggc aacagcagtt atgctattgt tccatttacc
     2941 agtagcaact gtgggtaaga ttccacaaac attgatcagt agtaatcgtt tgaatatggg
     3001 agattttagc ttttctgcct tgcaacaagt ggcagttcca gctattagca ttgctttatt
     3061 aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc cgaatggcaa ataagccctt
     3121 agatagcaat caagaattaa tggcgcaagg aattggaaat ctgttgcttc ctttctttgg
     3181 cgggattcct gctactgcgg ccatcgcccg tacaagcgtg gccatcaaat ctggtgcaca
     3241 aactagatta gctggtatga tacatgcagt tgtcttactg ctctccatgc ttgtttttgc
     3301 gccagtgatg tcacagattc caatgccagc acttgcaggt gttttgattg tgacggcttg
     3361 gcgtatgaat gaatgggaaa cgatcaagga gctatttact aataaatatt ggtcagcagt
     3421 tctattgttt atccttacga tgatttgtac agttatcttt gatttaagta ttgctatcgt
     3481 gatcggggtg atcggcggct gtgtattttt tgttgtcaaa agcgctgcta tcacgatttc
     3541 attagaaacg atcgattggc aaagaatggg gctgccagaa acggaaaagc tgaataactg
     3601 gacagtagtg tatattagtg gtccactgtt ttttatgtct tcagaaaaac tcaaaaataa
     3661 attggaagaa ctaaatcatc acgatggtgt cattttctcc atgcgaggcg taccaagtat
     3721 tgatttgacg gcacgtgcag tttttgatga atttcaagaa aaagcagagc aaagaggaca
     3781 aacgattatc tatacttcct tgcagccaga agtagaaaaa caactttctc acttgtggga
     3841 acatacattc acgcatcaac atcaaacagt tgctcatgcg ttgacagctt tacatgcgaa
     3901 atatactgca attagccatt aaaacaatga aaaagaacag acaagactgt aaactgaaga
     3961 ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa gtgaagctag tacttgccag
     4021 tcttgatgga taacaggtac aagggagcga ttgtagaaaa tagctgctgg atgaaaaagt
     4081 ggaagaattc gatagttttt ttctgaccat tcgtaggcat ccttttgttc tgttaattgt
     4141 aagacaggcc ctgtatagag ttgaccatga tttttggtaa ccgtatattc tttgccaagt
     4201 aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt ttggctggac cttcatcagt
     4261 tcgtaatcca atattggtgc atgcgcaaat acttcggaac gagtaggtgt ccgatttgga
     4321 taaacagttt ctgactgttg tgtccgttta ttgatccgat gagtcacgcg atacgggcgg
     4381 ctccttacag cactggtgat atacacctct tctcgtgtta agccagttga agaaagtgcc
     4441 tgcatcaatt cttttccagc ttgaccgcta aaaggaacat gcgtatcaat ctctgttttc
     4501 cctggggctt ctcccacgag catcagttta ggatgaagag gtccttgccc agcgataaat
     4561 ccttctacag gacggccgtt cattcttaaa gaagcttctt gtatcagttc ttttgggtat
     4621 tccatttatg ctcctccttt ttcttttatt ataagaataa aagaaacaca atgcgactat
     4681 tttatttggt agaactgtga ggatagaaaa gcagaagtat tgcttgaatt tttgttttac
     4741 tttttgattt taaatttagt tgactaacgg ttgctgttct ggtaagctag tactgttgta
     4801 attgtggact ccacagctac aaccgctcag aacaagtaat aagcacagca tatgctgtca
     4861 cttcggatgg cgagtctaag tatatcagaa ggaggtgctt tatagcatgt acgcaattat
     4921 taaaactggt ggtaaacaag taaaagttga agtgggacaa gcaatctatg ttgaaaaact
     4981 tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt atcttagtag gcggagaatc
     5041 tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt gaaggtactg ttgaaaaaca
     5101 cggcaaacaa aagaaagtcg ttactttcaa atacaaacct aaaaaacatt cacaccgtaa
     5161 acaaggtcat cgtcaaccat atacaaaagt ggttatcgac gcgatcaacg cttaatagcg
     5221 tttgagaaag aaggcctata tatgatcaaa ggaacattta agcgaaatga cgcaggtcag
     5281 atcgtttcat tcactttgac cgggcatgct gatgctggtc cttatggaag tgatatcgtt
     5341 tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg ggattgcctc attagccggt
     5401 tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt atctttatac cgaagtcact
     5461 tcaggaatga cacaagaaca aaacaatatt gctgagattc tattagaaaa tcttttatta
     5521 ggattacaat cgatagaagc tgaaaactca gaatatatcc aaattaaaac aattaatgat
     5581 aaataggagg tgcagaccat gttgttatct atgaatctac aattatttgc ccacaaaaaa
     5641 ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa aacgtctagg tgctaaacgt
     5701 gctgatggac aaacagttac aggtggatca attctttacc gtcaacgcgg tacaaaaatc
     5761 tatccaggtg taaacgtggg tatcggtgga gacgacactt tatttgcaaa agttgatggc
     5821 gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag tgtctgttta tccagtagct
     5881 caagaagctt aataaataaa ctgctctatc tcttgtgtag caagggatgg agcttttttg
     5941 taaattatgg gatttttttt gaccatactt ttgaccatac tttcttagaa gttaatataa
     6001 tttgttagtt tttgtgttgt ttcttctttt ttgctgttag taatgtgtgt atatgtgtcc
     6061 atagtagttt ttatatttgc gtggcccatt ctttcttgga cttctttgtg atctgcgcct
     6121 gcctcatata gcattgatgc atgtgtatgt ctaaaaccat gcagaccgat ataaggtaag
     6181 ccggcacgct cacgatatat attgtatctt tctgccactg actgattaat tgtgaaatta
     6241 ttttcgtcat tggtaaacaa ataattagct ttagtaaatc ctaattgaaa taagtacttt
     6301 ctttgttcga gcttccaaaa ttttagttgc tttatagttt tttcatctaa ataaattatt
     6361 cgatttgatt ttttggtttt aggagtttca gatatataat atttatctga acgagctaca
     6421 gtttttttaa tggccatttc atttgtttta aaattaatat tgtcccaagt gagtgctaat
     6481 atttcgccga ttctacatcc gctgaatgct aatagtctaa agagcgtata gtctctgttt
     6541 ttaaaatatg gattctgttc ttgagatact tcatttagaa ataattccaa ctgttctttt
     6601 gtatataatt ttaatttctt ttctgtttta atttgtggct ttggaataag taaatttaat
     6661 gttggattat cagatgttaa accaatattt atagcatact taaaaacttt attca
//
LOCUS       DAEGIQ010000127         6702 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000127 DAEGIQ010000000
VERSION     DAEGIQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.131"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..477
                     /locus_tag="KMT45_002482"
     CDS             <1..477
                     /locus_tag="KMT45_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE7005918.1"
                     /translation="LDREYPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTD
                     MIPRQIDLTVPYGYHVSEKGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPER
                     TLCDIWNPWYTVEDEIKVKAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            490..1389
                     /locus_tag="KMT45_002483"
     CDS             490..1389
                     /locus_tag="KMT45_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBE7005919.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1392..1523
                     /gene="ssb"
                     /locus_tag="KMT45_002484"
     CDS             1392..1523
                     /gene="ssb"
                     /locus_tag="KMT45_002484"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE7005920.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            1545..1877
                     /gene="ssb"
                     /locus_tag="KMT45_002485"
     CDS             1545..1877
                     /gene="ssb"
                     /locus_tag="KMT45_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE7005921.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            1899..2156
                     /locus_tag="KMT45_002486"
     CDS             1899..2156
                     /locus_tag="KMT45_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005922.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2315..2437)
                     /locus_tag="KMT45_002487"
     CDS             complement(2315..2437)
                     /locus_tag="KMT45_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBE7005923.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            2627..2851
                     /locus_tag="KMT45_002488"
     CDS             2627..2851
                     /locus_tag="KMT45_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005924.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            3299..3505
                     /locus_tag="KMT45_002489"
     CDS             3299..3505
                     /locus_tag="KMT45_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBE7005925.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3505..3756
                     /locus_tag="KMT45_002490"
     CDS             3505..3756
                     /locus_tag="KMT45_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005926.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            3772..4209
                     /locus_tag="KMT45_002491"
     CDS             3772..4209
                     /locus_tag="KMT45_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005927.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            4202..4897
                     /locus_tag="KMT45_002492"
     CDS             4202..4897
                     /locus_tag="KMT45_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005928.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            5278..5457
                     /locus_tag="KMT45_002493"
                     /pseudo
     CDS             5278..5457
                     /locus_tag="KMT45_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5519..5703
                     /locus_tag="KMT45_002494"
                     /pseudo
     CDS             5519..5703
                     /locus_tag="KMT45_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            5737..6168
                     /locus_tag="KMT45_002495"
     CDS             5737..6168
                     /locus_tag="KMT45_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBE7005929.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            complement(6342..>6702)
                     /locus_tag="KMT45_002496"
     CDS             complement(6342..>6702)
                     /locus_tag="KMT45_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBE7005930.1"
                     /translation="VDIENSINEWLQSVPKNLFKSITFDCGKEFSSWKSISNTNDIDI
                     YFSDPGTPSQRGLNEHSNGLLRKDGLPKEMDFNQVNQGFISSVASKRNHIPRKSLNYQ
                     TPLEVFLSYVNGKFCLA"
BASE COUNT         2355 a         1114 c         1358 g         1875 t
ORIGIN      
        1 ctggatcgag agtatccagg ttttttcacc ttacctggtc aatttcctga tgaattgttt
       61 atggcgcaaa aaaaatatga acgcggcatt atttcacata ttaccgcgtt agatttgtat
      121 gatttaacag atatgatccc tagacagatc gatctgactg taccttacgg ttatcacgtt
      181 tctgaaaaag gattgaagga attcgcagtt gagctacact atacaaaagc ggaatggtat
      241 gaattaggta agattgaaat aaaaagtcgc tatgggaatc ctattatagc ttatgatcca
      301 gaaagaactt tatgtgacat ttggaatccc tggtataccg ttgaggatga aatcaaagta
      361 aaagcaatca aaaactatat ggagtcggac agaaaaaatc ttagaaaatt gaatgagtat
      421 cgacgaattt taccaacaga caaaacgatg cgttcttata ttatggcttt aaattaaagg
      481 agaagataca tgacacctac gcagttaaaa gcaaaagtga agaatatttc tagagaaaaa
      541 gaagttgatc cgcagcttgt tttaagacat tttatgatgg aaaaattctt ggaaaaaatc
      601 tcagagtctc cttatcgtga aaattttgtg ttaaaaggcg gcttcttgat tggctcaaag
      661 tatggcattg aaaatcgcac aaccaaagat attgatacga ctttacggga aatgaaagta
      721 accaaagaaa cattaacaac ggttctaaac gaaatttttt caacgccgac gaaagaaggc
      781 attcaatttg agattcaagg aatgaaagaa acgagagaag ccgattacta tccaggattt
      841 agcttacgtg tgctggcaca tttggaaaac atgcggccag attttaaggt agatgtgact
      901 acaggtgact cgatctatcc tgcaacgatc actcacagtc ataaattgat gtttgaggat
      961 cgaacgattc ctttggaatc ctatcctaca gaacaaatta ttgctgaaaa attaagtgca
     1021 acctttgatt ttttaacaga taattcgaga ggaaaagatt tttatgatct ctatactatt
     1081 cctaaaatgg agaagatcga tgagaagacc ttgaaagata gtgtgagaaa tacatttaca
     1141 cgtcgaggaa aaactgatcc gttaaagata tactatgaaa aggatatgga tgtactgaaa
     1201 aatagtcacg agctaaaaga tacctggaag aaatatcaaa aaagcaatcc ttatgctgcg
     1261 tcgatcacgt acgatgagac aatggattcg attagtaatt tgatggaaaa agtgattcac
     1321 gtcgaagaaa aagagcgaaa actagcttat caaagacacc aacagatgaa tagagaaatg
     1381 gagagataaa gatgattaat aatgtgacat tagtaggacg cttaaccaaa gatcctgatt
     1441 tacgttatac cgcaagtggt actgcagtag cgacttttac cctagccgtc aaccgaaatt
     1501 tcacgaatca aaacggcaat tgagaagccg attttattaa ctgcgtgatt tggcgcaaac
     1561 cagcagaaac aatggcgaca ttagctaaaa aaggtagctt gattggtgta gttgggcgga
     1621 ttcaaacgcg gacttacgac aaccagcaag gacaacgtgt atatgtgact gaagttgtag
     1681 cagataactt tcaactatta gaaagtaaag cagcgacaga gagccgtgcg cacgctgatc
     1741 aatcgtccac cagtccaagc accgccacct ttgaacaacg gaatacagcg acgcctaaca
     1801 acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat attagcgatg
     1861 atgatctacc gttttagttt gagaaagaag gagtaagcat ggcaaaaaaa attagtctga
     1921 cgatggatct attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg
     1981 aaaatccttt ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg
     2041 tctgttctta tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag
     2101 tcacagtagc agcgacagct gttatactta cggctattct accgctttat atgtagagtt
     2161 aacgggaaat cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt
     2221 tgcgctaaca acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat
     2281 ttggaaataa ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca
     2341 tatttacaat caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa
     2401 ttgtctcata tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata
     2461 agcaccacca cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat
     2521 agcatatcta aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg
     2581 aggtgatcgt gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca
     2641 gccattgctt gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc
     2701 caatatagaa atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt
     2761 gacaggtcac ttcaataaac gaaatcaact agtcattgaa tcaatgcaaa ttagaaaccc
     2821 tgatcatttt acgagattaa tgggcatata aataaaagaa gagctgcagt cctaaatgag
     2881 gattgcagct ctttttgttt tacatagaaa caagaaaaat gaaaaggcct ttttaggaag
     2941 gcgcaagcgc cttctcaatt gaaaaacctc tctctcactc caaccgccac cgccgttcga
     3001 gaagaaagga agtgaacgtt gcacgttcac taagataagg ggtcttgcaa agcaagacaa
     3061 aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgctatctc tttattgatt
     3121 tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca agggtaaatg
     3181 gcacgctgca cgcccttgtc cttgtcgctt ttagttgatt cattctggcc aacaaatcaa
     3241 taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag aatcaatcat
     3301 gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa actatcatgc
     3361 accatatact gtcgaatgcg attgtggaga aagagcgaaa tgtatcaaaa gctattgttt
     3421 gtatcaatgt ccaacatgcg ggaaaaaata taccttgtct tatggcgatt atattttgat
     3481 tgatgagaaa ggaaagaaac gctgatgaaa acacaacaca aattattgac tgtaaaagaa
     3541 gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga agctttactt
     3601 attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac tatgctattt
     3661 gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat ggaaacttat
     3721 gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa tatggctcaa
     3781 aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa aatggaggat
     3841 atgacattcc tatacaatga gacacgtgtt ccaaaaaaac attataagga aagattgtcg
     3901 gtagctgtgg aagaaatgat tgaatcgggt gtcgaaatga atctcattgc gacctactat
     3961 cgaacgcttg aagaactgaa aaagcaaaat ggacaatggt ttttccaagc cttactatgc
     4021 ttggaagcag gcgtcaagcc aaacactatc aaaccttctg aataccaagc tttagaacta
     4081 acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga atggttggac
     4141 tacttagaaa atgtcaacaa gtatggcgca tgttacatca tgaaaaagga ggacaacgaa
     4201 aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt taacaaacaa
     4261 agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta tggaagaaat
     4321 gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa cgagtcaaac
     4381 gaaggttaca gtcagcaata attatttaga cgtgtataaa cgtcgtcctg acacattaaa
     4441 aaaaggagcg gagcttctac gactagaaac agaaaaatat atcatgaatg tttacctaga
     4501 aaggagtgaa ctggtcgaaa actttgtcag ctacacgatt tgcaggaaac actattactc
     4561 agaatcagaa tattattcaa tttttgagag catggaatta atggaagagc taaacagcaa
     4621 agatgcgacg gtagacgatt attaccttgt atttactaaa tttttgacgt gtgggacgga
     4681 atctatcgtg ttaatgggaa tggattatga actcgctgta cccgaagtat ttttgaggga
     4741 atatgtagaa gaagtagata acccaggcga cgactggtac acgactttaa ctgatttttt
     4801 agatacctac acttacgacg atacagaaga aatccaaaat gaacttgacc gtaaacgaat
     4861 gccatatcaa cgatttgaaa ggaaaaaagg tcaataacca tttataccga aaagtgagaa
     4921 tgtgtgaaat aaaaacaaaa aaccttgaaa ttaagggaat ggttcttatt tcgcaccaat
     4981 cgctccaacc gcccccgccg cttaggtggt gcgaaataag cgagcaaagc ccgaaaacct
     5041 aagagaaaga aggagattta tatgaacaaa attattccct tattattagt cctatttgtt
     5101 ggagaaatac ttatgcgact tagtgcagct gtacatttaa acctcgcagt atttttggta
     5161 atactaatgt tttgtttgat cttttggcga tttgtattag ccctcgtaag tctactattc
     5221 aagatgattg cagccatatt tttcatagga ttatttcgat gaaaggagag agtaccaatg
     5281 ccaattgcaa taggaaataa acggttaccc gtgacgttgg atgaaaagag acaaaaagaa
     5341 ttgcagtaac caaagcagaa gtacggcaaa agtgaatcca ggattatgtg tattgcgtta
     5401 gatttattga ttgcgcaaga aaaagcagga tttgacatac cagcactcaa aaagtgatcg
     5461 cactttgcgc acgtgttaat acgtgttata attattttgt aaggaggtat agtgcacaat
     5521 gccacttacg ggaaaagaaa tgcttaagtt attgaaaaaa atggctgggt tgagcgaagg
     5581 caagagggat ttcatcatca tctttataag gatggtgtta gaatcactgt tcctgttcat
     5641 gccaatcaag atcttggccg aggacttgag cgaaaaattt taaaggatgc gggattgaaa
     5701 taatctcgca ttcgattaaa gaaaggattg actgaaatgt taagtgagcc tttagcaaaa
     5761 acctatcctg ctatttttag cccagaagaa ggtggtgggt actttattga atttcctgat
     5821 gttcaaggtg cttacacagg aattaacgaa gacgatatat cttatggaat tgctatggca
     5881 caagaggtgt taggtatggt tttagcagat tatattgagc atgaagatct acttccagaa
     5941 ccaacaccaa tcaataagat ttctgttgaa gatgattctt ttactacttt aattcgtgta
     6001 gatgttgcca aatatttgaa agacacagaa cttgtcaaaa aaaccttaac aattcctcaa
     6061 tgggcggata agctaggcaa aagggctggc ataaattttt cagtccttct aacagaatca
     6121 attgcagata aagcagataa cattcttcgt tctggaagaa ataattagaa ctcaaaaggg
     6181 tcagaaactt aaaaatcttt ggcttctttt tttactacaa agatggcttc tttaaattat
     6241 ttgcctgatt gatttaaaaa aattgcgcaa agactacgag ctaattaagt tgctcgtggt
     6301 ctttttttat gaaaactttt tagatcttat tatttgtcaa attaagcgag acaaaacttt
     6361 ccatttacgt aactcaaaaa aacttctaat ggtgtttggt aatttagtga ttttctaggg
     6421 atatggtttc ttttggacgc aacggatgag atgaatcctt gattgacttg gttgaagtcc
     6481 atttcttttg gtagtccatc ttttcggagg agtccgtttg aatgttcatt taatccccgt
     6541 tgggaaggcg ttcctgggtc tgagaaataa atatcaatat cattcgtatt actaatactt
     6601 ttccaactag agaattcttt tccacaatca aaggtaattg atttgaaaag atttttgggt
     6661 acggattgaa gccattcatt aatcgaattt tcaatatcaa ct
//
LOCUS       DAEGIQ010000128         6455 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000128 DAEGIQ010000000
VERSION     DAEGIQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.132"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            269..964
                     /gene="vanR"
                     /locus_tag="KMT45_002497"
     CDS             269..964
                     /gene="vanR"
                     /locus_tag="KMT45_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBE7005931.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            942..2096
                     /locus_tag="KMT45_002498"
     CDS             942..2096
                     /locus_tag="KMT45_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBE7005932.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(2194..3486)
                     /locus_tag="KMT45_002499"
     CDS             complement(2194..3486)
                     /locus_tag="KMT45_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBE7005933.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(3644..4954)
                     /locus_tag="KMT45_002500"
     CDS             complement(3644..4954)
                     /locus_tag="KMT45_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBE7005934.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(4966..6384)
                     /locus_tag="KMT45_002501"
     CDS             complement(4966..6384)
                     /locus_tag="KMT45_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7005935.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         2027 a         1264 c         1200 g         1964 t
ORIGIN      
        1 tactaatgta tctagggctt cattatacag gaaattatca gaagtgaata attagccatt
       61 ctgtattccg ctaatgggca atatttttaa agaagaaaag gaaactataa aatattaaca
      121 gcctcctagc gatgccgaaa agccctttga taaaaaaaga atcatcatct taagaaattc
      181 ttagtcattt attatgtaaa tgcttataaa ttcggcccta taatctgata aattattaag
      241 ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg tggatgatga
      301 acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata cggttttcaa
      361 atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg accttgccat
      421 attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa taagggacaa
      481 gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata aaattacagg
      541 gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg agttaattgc
      601 tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg agcagaacga
      661 aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt gttatctgaa
      721 cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc tctgtgaaaa
      781 caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg acgaatattt
      841 cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa aaatgaacga
      901 caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata aaattgaaaa
      961 ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc gttgcaattg
     1021 ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg aaacttgggg
     1081 attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac gcgatgaaat
     1141 tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg attgtcatta
     1201 gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt gacgagataa
     1261 ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt tctgcggaaa
     1321 tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag cgagagcagg
     1381 atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg cacgatatta
     1441 aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct ccagacatgc
     1501 cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat cgactcgaac
     1561 agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata acgctaacaa
     1621 aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt tatcctcagc
     1681 tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc gtgtccggcg
     1741 accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct gcatacagtg
     1801 aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg tcaatcgaat
     1861 tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa aagttctata
     1921 ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg gcgattgcaa
     1981 aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat aactatacga
     2041 cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg tcctaagaga
     2101 tgtatataat tttggctctt tgtcaaatga tgttggtaaa gagaaatctt agaataattg
     2161 gagatcaagt ggatcagttc ctcttgatct tttttagttg aaaatcaatg cgttgccatg
     2221 acgaatcatg atgcgcaatt tgaaattata aaagctacga aagccatatg catttcgttt
     2281 taacactttg atatggttgt tcagacattc aagtgggcca tttgaataac tttgttgtaa
     2341 agcatttttg atttgtggca gaaatttctg gaacgttctc aaggtcgtct gatagccttc
     2401 gggaagcatg atattattgg taagtagttc ttcaaacgat acgtagtctc gttcctttac
     2461 agcgtaaaga aagtcttgat ataactgata gcctacacgc aaagcagggc tccctttcag
     2521 taagcgatcc acaatatcgg tctctgttaa atgagctttg aagccaggtc gccaaaggcg
     2581 tcgagtataa tcaagcttgc gttcatcttt ttgaagaagt ttccagtatc tttttaattg
     2641 cttacctaat tgatagtgtt tttgttcttt gctttttaat agttggttcg tttctttgat
     2701 tcgatgattg cgaaaggtac gaccaatatg ttggacgatg tgaaaccggt caataatcag
     2761 ctgtgctttt ggaaacagct ttttaacgag cgatacataa ggagcgtaca tatcaatcac
     2821 gatccattcg accgcttcac gaatttctcg tgaaaaatga ctaaaataac gttttaaaaa
     2881 aggcagttgt cggttctcga caatgtccaa taagcgctgt gtttgtccat ccatcatgat
     2941 gaaactcata gcacctgaaa caccccgaac tgatttgaat tcatcaaaac aaagtacctt
     3001 aggtaacggt gtccgaaaag gattcaacgg acgataaaat cgcttcataa tacggtaaac
     3061 agaagagacc gacagatttt tctgagcggc gatctctgtc attgaggtat tttttttcag
     3121 acgagtcacg acagaccagc ggactttctc actgatgaaa caatgttttt tcgcaacaga
     3181 agtatctgcg acaaaggtcc gctgacaagc tttgcatttg aagcgttgct ttttcaattc
     3241 gaggtaggta cggtattcag aaacatcgtt cattaacaac cggacggttg ttgtacccca
     3301 acgcacaata tctgaagcaa tctggtgttt acagtggatg cattcttggg ctgtataaat
     3361 caagtttcca cgataaatat gacagatttg ccctttgtat ttttcttttg tgagacaatt
     3421 ttcgttaaaa gtaactgata agtctagaat atctaatgtt tctttgataa ggtgagtgta
     3481 agacatcttg attcctcctt ttgggttggt tcgccaaatt aattctaaag gatttcaagg
     3541 tgtcttttct atatgttttg gaaaataaaa aagatgctgg tgtcatccac caacatcaaa
     3601 aagtatagag ccaatattat ttaagcttga ccttattata atttcaacga ttgtcaaaag
     3661 tattgtttgc agctgtgttg ccagtttcta tagataaatc acagcttttg aaacataagt
     3721 tttgtactgc ttcgattcgc ccgaattctt ttgcagttat ttggcaatga gaaaagcaga
     3781 catcttctat aggcttttca ggaaatgcta ctaaatcaaa agctcgggaa ggaagtgagt
     3841 aacctggaga aaggcatgcc tgtacgtttt tgatgtgtat gtctttgact ttcgtcattt
     3901 gctttgattc aggtatttct tcagccactg cttgccaact ttctggcatt tgagcaaggt
     3961 cattgctatt ttttctattg tattcattat gccagtccat tatccaagaa aaaggaaatt
     4021 gtacatcaat catattcaga ttttctaccc tgatattttc gatcactccc cctctttctt
     4081 ttgaagattt cattcgaata ccacagtctg actgaaaaaa attgatatcg tgaatatata
     4141 cgtcactaac tccagcacta acttcgctac caatagttac accgtatcct gaacggattt
     4201 tacatctagc aacctcgatt cttgatgatt ttcgattgac tcttgcacca ttgccatctc
     4261 tgccagactt aatagcaata caatcatcac cacaggccaa atcacattca tagacacgca
     4321 catttgttga agaatcaata tcaatcccat ctgtacttgg tccatcattg ttgcggatga
     4381 ttactttttc tactaataca tcgttagaat aagtgatttg caagttccaa aaacctgatt
     4441 tctgaaaaat caaatcacga acaacaacat gttctgcatg atacagtaaa caagcgcgtg
     4501 gacgtttaat agcataatcc gcaatccaac gaagtccctt ttgatcgtat tcagctcttg
     4561 tcccgctttt ttgatctttt ccccaataca gctcccacca atggtctcct tgaccgtcga
     4621 ttattccttt tccttctata agaatatctt tcgccgagag aacattcaaa atcgctgctg
     4681 gccatttcat ttctacgccg gctactcgtg tatcgatttc tggaaatgct tcgatatctg
     4741 ttctcccctt gaggactgca ccttcttcaa agataagatg actcttatct tttaaaaaaa
     4801 gggctccgac taaataaatg ccttttggaa taaatacagg taagtgttct ttcgcacctt
     4861 gatcgattgc tttttgcagt attattgtat catcttgttg agtgtcgttt gttttaaagt
     4921 tcaaagcatt gataatccct ttttttgtcc gcaatacgtt tctccctatc tttcattgat
     4981 ttttttcaat tcttcaccaa taaatacagt ttttgaacga ttcagtgggt aacctactag
     5041 caaaatgatt gcggatacaa ataagcagaa tgctggtaac aagtttgcca tcgtataaat
     5101 cctttcttgt actaaaactg attggatggc tccacctgtt gtagacgatt ggtaaccaat
     5161 gagtgccagc ataaatccgc ctaccgctcc tgcacatgct tgtcctactt ttctcgcaaa
     5221 agaatttact ccatatactg ttccatcttc tcttaagcct gtcttatatt gatgataatc
     5281 aatcacgtca gtaatgaatg cccagaccat caaattgaat aaaccagatc ctaaagtagc
     5341 gataaataaa agaatcagat atacccatgc atttgttata tgaaggaaat acatcagcaa
     5401 gtacatacat gaagaagcaa gtaacgcaaa tacgcttgct tcctttttac caaataattt
     5461 tgtcagcttt gtagcaaatg gtgctaaaac tactactgtt ccgtaagtaa ataaaagagc
     5521 aacagacatt gcttgtttat tatggaagta atcattgaat aaataagtca tattcgtccc
     5581 agctaaaatc tgattgatca caatgaaaat gtcaacaatg actaaaataa tcagtgctcg
     5641 attagctaac aaacctttta ccaactgaga agcagatact ttttcgtttt tgacaactct
     5701 tactctttca gtagtcaatt tacaagttaa catatatgcg acaaatgcta ttgcagcaca
     5761 tccgattgaa atccaaaaga aatgattccc tgaaacgact tgttgtccac tcttgttatg
     5821 ggtatacatt aaaatcggaa tgaagaatcc tgtagttgat ccgccaatcg cagaaccaat
     5881 gcttcggaac gtggacaacg aggtgcggtc attaggattt gagctaattg ctgctgccat
     5941 tgctccatac gggatattga ttgttgagag aaaaatccca taaacaatgt atgaaacaaa
     6001 aatataagca atcttcaccg tcattggaag atgttggacg aatggcaaaa acaaaatgac
     6061 agtcactaag caaaatggat acttcatttt tctgatccaa ggaataaatc ggccagtttc
     6121 tgttaactct gagacatcac ataatcttcc aaccatcaca tcagcaaacg catccaaaat
     6181 tctcgaaatc aagaataacg tacctacgat tgcccctgaa acgccaagaa cattcgtata
     6241 ataaatcatc aagaaactat tgactaaacc taaaataaaa cagttaccta gatcaccgaa
     6301 catgtagcct atcttatcgg aaatcccaaa ttcctttttt tcttttttta ctgtatttgc
     6361 tgtaacagca ttttgtgaaa gcatttgaat tcctcctacc tcttctagta aacgttttca
     6421 aaattataac tctttttttc ttgtttttat attca
//
LOCUS       DAEGIQ010000129         6441 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000129 DAEGIQ010000000
VERSION     DAEGIQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.133"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..2632
                     /locus_tag="KMT45_002502"
     CDS             <1..2632
                     /locus_tag="KMT45_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HBE7005936.1"
                     /translation="NTLGISYVNLRTFSTLNKKTVSHIFSTADSQAIINYLEKQKLSE
                     GTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVM
                     EQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVED
                     EVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIV
                     EENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDEC
                     PLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLES
                     NQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWD
                     EIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKEN
                     EAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIA
                     SIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAI
                     IKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLT
                     LTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLL
                     KLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRL
                     EHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKV
                     SIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNR
                     QVLPTYEILLPSSLKSYFRL"
     gene            2814..3053
                     /locus_tag="KMT45_002503"
     CDS             2814..3053
                     /locus_tag="KMT45_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005937.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3069..4442
                     /locus_tag="KMT45_002504"
     CDS             3069..4442
                     /locus_tag="KMT45_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7005938.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4446..6080
                     /locus_tag="KMT45_002505"
     CDS             4446..6080
                     /locus_tag="KMT45_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7005939.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6243..>6441
                     /locus_tag="KMT45_002506"
     CDS             6243..>6441
                     /locus_tag="KMT45_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005940.1"
                     /translation="MKKMGKCILCEKYTQLTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2254 a         1053 c         1296 g         1838 t
ORIGIN      
        1 aaatacactg gggatttctt atgttaattt gagaactttt tctacattaa acaagaaaac
       61 cgtttctcat attttttcta ctgcagattc tcaagcaatt attaactatt tagaaaaaca
      121 gaaattgtct gaaggtacag taaacataac ttataatcct attaaacaag aggtgattac
      181 acaaaaaaag cattctgtga gagacaatga tattgaaaaa attcggtttg tatttattga
      241 tattgaccct aaaaggaaag agggaagtgc aacagatagc gaaaagaaaa aggcagaaaa
      301 tgttatggaa caagtagaac gttatttgaa agaaaagaga atagaatcat ttgtaaaagt
      361 tgatagtggg aatggctacc atatttttct tcccataaat gaacaaccaa ataatcacga
      421 gacgattctt actattcaga attttcttca attacttcac cgaagatttg gagtagaaga
      481 tgaagttgat attgatactt ctgtttataa tccttcaaga ttatgcaaat tagttggtac
      541 accagcggtc aaaggaaagt ccacaaaaga gcgtccgcat cgtttatcta gtttcctttc
      601 tattcccgat aacatagata gaagtaattg ttttaaaatt cttgaacaga tagttgaaga
      661 aaattccatg atgtcaacct ctagagtaaa aagccatcca tcttttagag aagtgttacc
      721 ttttattcaa gcagactata aacaatggtt agctcactat cctgaattaa cttataccat
      781 aaaagagggc gatcataaag gagttacctt acttatattt gacgaatgtc cattaagaaa
      841 acacacgaat aattctaatg gttcttgttt gtctgttacg gctgataata agattcgatt
      901 tcattgtctg catgcttcgg ataaaaataa agatattcaa gactttgtaa aaaaatatcc
      961 gttgccaaaa cctgtaaatc cttctgtaga atcattagaa tccaatcaag aatattattt
     1021 tgaccagttt aaactgaatc aagaaggagt atttttggtg aaagaaggtc aagaagaaaa
     1081 agttgcttgt ccgatgttta ttaagcaaat acgcaaaaat atcgaacaaa aagtcattac
     1141 acttgttctt gcctattatg tcgatcattg ttgggatgaa atagaaatag aaggtggatg
     1201 tttacaaact aataatttta aacaattagc aaaacatggc ttaatcatta aagctagaag
     1261 agaagctgaa gtcgttgact ttttacagat tcaacgggag aatgctccaa tcttttttga
     1321 acatgaatct cttggctgga aaaaagaaaa tgaagcaatg atttttcagt tacaagattc
     1381 ttttggacgt gaaattcctt ctgtattaac taaaaactca tgctaccaat taacaactaa
     1441 tggtacagaa gcggaatttg acgcattggt tcataagtat gttttaggaa cgaacttaga
     1501 acttgcttgg tcgattggta ttgcttctat tgtgttaggc tatttaaacg ttacgaaggc
     1561 taatactttt ttgagtttac taattaacct acagggcaag agcacaacgg gtaaaaccac
     1621 tacgttacac tttattgcct ctttatttgg aaatccaact acggttctta gaaatatgaa
     1681 tgctaccaat aaagccatta taaagctagc atcaaacaat cagggaggtg ttccactaat
     1741 actagatgag ttaggagcag caactcaaaa taactttagc tcgttgatct atcaattagc
     1801 ttcaggagaa gaaaggctaa gattagattc taattgtcag cttatgccac aagagacttt
     1861 ttctgttttg acccttacct cttctgaaga gcgtttggaa aattatttca atgagaaatt
     1921 aactggtttg aaagttcgat atatcgaatt ttctgatatc aagtttacaa aggatgcgaa
     1981 tcacgcagag aaagttaaaa gtctttcttc taatactttt ggttggggta taaagttatt
     2041 tcttttgaaa ctctttgaac aagggattac aaaaattgaa gcaacttttg tcgaagctca
     2101 aaaaaaacta tgggctgttt tacctgaaca ttctttgaag caacgcattg gtaataatct
     2161 agctttgatt tatagtggag ccctcctaat tcaagatata ctgggctatc ctttacgatt
     2221 agagcatata cagacggtgc ttattaattc gtacgaacag ttaattgtag atactagttt
     2281 aggaagtgag caaacggtat ttgaccgagt gaaaacattg ttggtgatga atagccataa
     2341 atttttaaca aacagtgaca acaaacatac gcccagtacg atttggggaa aagtatcaat
     2401 tcaaaaaaat ggggatatta aggtaaatat ttttcctatc ccgtttgagg acttgttaag
     2461 gcgagaattt caagtaaagg atctaaccta tctttttagg caattgcttg attcgggtaa
     2521 tatgtgtgca gaaaaaggga gaagaacgaa acgtattcac atgaatcgtc aagttcttcc
     2581 aacatacgag attctattac cttcttcact caaatcgtat tttcgattgt aatttagaat
     2641 aaaccaaagt atgaaagtga ggcaccatgt tatggaaata tttcttattg atttagcaaa
     2701 acaactgatt acttatctgt atgatagcga gcaaacccaa acggaagtaa atgaagcttg
     2761 atctatgctt catttacttc attcaatcaa acatgaaaga aaggaaaatt ctaatgacta
     2821 aaaataaaat gacactaaaa gcagaggttc ttctgtatat tcaagaacat ttttcaaatc
     2881 aagccttttt tacaaaacca atttacttgg atttcgaaat aagaggggta tctgctggat
     2941 ctatcggggg tactcttcaa gcgttgaaga acgaaggata tttagaaaat cactttgtgc
     3001 aacgttcgtt caatagaaga gtcgtgaaaa aatggtattt agttcattca tagagagggg
     3061 aatcaagcat gcgtgtggct tgttatgatc gaacgtctgt ttatgaagaa gcgaaaatta
     3121 gtaatgacgc tcaatatgat gcagtgaaag agtatgtttt agagaaatat gaacaaattc
     3181 cccaacgatt ttcagacaaa ggagaatcag gggaaaaagc ggagcgtcca gcttttcgac
     3241 agcttagagc ttgggtttgt gcgtcagaag aagagggggt gatctatgtt tggcgttatg
     3301 atcgattggc acgtcaagcc aaaatagcct tagaatttat tgagttgtgc caacaacatc
     3361 aggttcaagt gtattcgatt tctgaacctc ttcctgcttc tttctcacac gcgtcaacga
     3421 ataaactatt tgtgcaatta ttgttcactt ttgcagaaca acaacgaaac gtcattattg
     3481 agaatgttcg tgccggactt tcctataaaa agcaacaacg gcaatatttg tcttctgcgg
     3541 taccatatgg gtatcgttta gtgaatggga aaatccaaca agaagtacac gaagcaagga
     3601 ttgttcgcta tatttttcaa ctttatttga ccaaaaaata tggctacaaa aagctttgtc
     3661 aacgattgac gcaacaaaag cttttcttcc gcgaacgtcc ttttcagccc tatcatattt
     3721 atagtatcct aaaaaatcca ttgtactatg gcgaaatcaa aggaggatct ttggggaagt
     3781 atctaggaac ctttgaaccg attctctcga aagcaacctt tttacaagcc caagagattc
     3841 gccaatcaag gtgtacagct aaaaaagata cgtatctgta cttattacgt caaaaaattc
     3901 ggtgtccctt ctgtggtcgt catctgagtt cgaagtatca atggaataca aagaagacga
     3961 agacgcttca ttattatcat tgtaccgacc gaagctgcca aggaattttt cttcgggcag
     4021 aaagggttga gtcagaagta ttggaaaaga tacaggcttt ttttcaatac gaaggcgtct
     4081 atcaagcctt gattgcccaa ttaacgcaac aattagccga aaagcgaaaa gatgaaaaac
     4141 gcaaagtaat ccaacaaaag aaacaagaag cacaatggat taaggcattt gaagagggca
     4201 attgttcact ggaagaactc aagcaacaac tgcaagctct tcaaaagccg atgtctactt
     4261 cttcgtttct ttcactccaa cagtgtgaag caaaagtgaa agaatggctt caactaaaag
     4321 acctctctag tcgacaatta ttatgtgact atgtttgtcg gattgaggta acaaaggata
     4381 aaacagtttg tgggatttac ttccgacaac tgacacaaaa tattttagaa aggaacgaat
     4441 gatctatgga aatgatacaa accaaacgag tcgcattgta tattcgcgtg agtacacaag
     4501 aacaagcttt agaaggacat agtattcaag cacaagaaca aaatggacga cagtttgccg
     4561 aacggatggg ctatgaagtg gtagaagttt atgccgatga aggaatcagt gggaaatcca
     4621 caaaaaatcg gttcgcctat caacgaatga tgaacgatgc tcggaaccat cgttttgatc
     4681 tactagtgat ttggaaattg acccgtctcg gacgcaatat gttagatgtc ttacaaacag
     4741 tcgaggaatt attgcgatat gacgtaggat tacattcgat ctcggaacaa tttgacgtga
     4801 ccacttcgac aggtaaattg atgttacaac tccttgggag tttcggtgaa tttgaacgca
     4861 accaaattgc ggagaatgtt caaatgtcaa tgcaaagctt agtgaaagat aaaaagcgtt
     4921 atgcaggagg ccgacgttta ggctatgtca gtggtattga cacgaatggt caaaaacaat
     4981 tggtcattga accagaagag gcgaaaattg ttcgtctgat ctatgcaaaa tatttaggcg
     5041 gagcagggta tcgagcgatt gccaattttt tgaatcgtca aggatatcaa acagtcaaaa
     5101 agaatgcttt ttctaccacg gcagtcaaag atatcttacg caataaaatt tatgcagggt
     5161 atttagaata tgcgcgctac atcaattggg aaaccaaacg acggaagggg aagaatccga
     5221 gaccgatttt agtgaaagga acccatgaac cgattatttc ggaagaagat tatcaaaaag
     5281 tacaagaacg ccttgcttta gaaggaaaac aacccaaatg gaaccataca ggtgaaaatg
     5341 tattgacagg cttgttacgt tgtccagaat gtggcgcacc gatggcagcg agtaatgtca
     5401 caaataccct aaaggatggc acaaagaaac ggattcgata ttattcttgt tcagtctttc
     5461 ggaataaagg agcaagtgtg tgtcatgcca attcgattcg tgccgaacaa gcggaaacat
     5521 ttgtcgccga ccgattaaag gagatcgttc agctaccaga agttcttccc cgattggtgg
     5581 cagcgttaaa tgaagaaata gtgcgacaaa gccaaccatt ggaacaagaa ctcgtggtgt
     5641 tactggaacg aaaggaagaa ttgaagacta aaattgaaaa atgggaagct gctttggaag
     5701 atagtcctga attgtttccg atgttaaaag accgcttaga cgaattgact gaaaaacgac
     5761 gacagttgca cattcgagaa aatgaaatct tagggatctt tcaacaagga gagccgattc
     5821 aagtcaaaga tgtccaacgg atattgacta gtttggatcg ttttcttgcc cagagtgaga
     5881 agaagcaaat caaagccttg tatcgtacct ttatagagaa aataaccttt gatcctaacc
     5941 ataaggagat gttggaaatt acgatgaaat tcacgcatgc cgtggtacaa caattgaatg
     6001 agcaatatca aatagccgta tccaaaacaa aggatacggc tatttttgtg ttaaaaacac
     6061 cctttacgtt aacgatctag tctaggttat atcaaacaac gttagagggg cgctaacgct
     6121 tctctagcga tttgaaatcg ttagagaagt agttagggag gtccttatca taaataggga
     6181 tatttattaa tgaatatttg aaatataatt gtatataaga attaaaaaga gtggggatag
     6241 atatgaaaaa gatgggtaag tgtattttat gtgaaaaata cactcaactt actaaagaac
     6301 atgtaccacc taaaaaatca gggaatactg gaggtaaaaa gaccagaact ggtaatttag
     6361 atgatttcct taagagcgat tttacaaaag gtgattttcc gaaggggatt aaaaggaaac
     6421 ctcaaggaaa tgtttattat a
//
LOCUS       DAEGIQ010000130         6440 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000130 DAEGIQ010000000
VERSION     DAEGIQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.134"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(47..697)
                     /locus_tag="KMT45_002507"
     CDS             complement(47..697)
                     /locus_tag="KMT45_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005941.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(797..1306)
                     /locus_tag="KMT45_002508"
     CDS             complement(797..1306)
                     /locus_tag="KMT45_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBE7005942.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1507..2376)
                     /locus_tag="KMT45_002509"
     CDS             complement(1507..2376)
                     /locus_tag="KMT45_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBE7005943.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2396..3517)
                     /locus_tag="KMT45_002510"
     CDS             complement(2396..3517)
                     /locus_tag="KMT45_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBE7005944.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3519..3914)
                     /locus_tag="KMT45_002511"
     CDS             complement(3519..3914)
                     /locus_tag="KMT45_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBE7005945.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3933..4739)
                     /locus_tag="KMT45_002512"
     CDS             complement(3933..4739)
                     /locus_tag="KMT45_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE7005946.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4732..5520)
                     /locus_tag="KMT45_002513"
     CDS             complement(4732..5520)
                     /locus_tag="KMT45_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7005947.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5534..6010)
                     /locus_tag="KMT45_002514"
     CDS             complement(5534..6010)
                     /locus_tag="KMT45_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7005948.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6082..>6440)
                     /locus_tag="KMT45_002515"
     CDS             complement(6082..>6440)
                     /locus_tag="KMT45_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE7005949.1"
                     /translation="NNILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFD
                     NNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVD
                     IPIHEVKYVYDYVKRA"
BASE COUNT         1995 a         1382 c          908 g         2155 t
ORIGIN      
        1 ttccattttt acagttataa ctagtattat catgaaaaat agccttttat tgtctatggg
       61 aataataatt taaaattggt acttttttat taattagttc atcgcccaat aatctctcca
      121 gtttcttagc atctcctgac tcaaccggaa ttatccaagc ccagtctaaa taatttttta
      181 aaatctcatc atattttgca aatatttcat tgccatattc cgtccttaaa attatatttt
      241 cagtgccaga caagtgattt atattgagtt gataaaaaag atctccatct aaaaaaatat
      301 cattagaaga agatttcccc acagtaatga ccattccatc agctctatgt gttacataca
      361 aataattgta tgacctcatt tcacctaatg aatcaattat ttcttgccct ttagctctaa
      421 tatattgatc tctattacct atctcactct tatttaacaa aagttttttc atgtcaaatt
      481 taatcggctg ttttattttt gtaaattcgc ttctactact aatccaatcc gagattaatt
      541 tttcaacatg tcccagagta aactctgatt tattcgctaa atactttttt tgttgtatta
      601 aagacatctt tccccgaagc tcaaaattga gcctacatcg attctctatc tcttcaaaaa
      661 gcatcttatc ttcatcattc ttatattcat cattcataaa accaatccag ctcctcgatt
      721 aagataatat tttaatctca tacaaaaact agctctcatc aaaacaacca ttgttttgat
      781 ttctcatgaa atttctttat acttttctcc gattataaat tcctaacccc ttgagaaaat
      841 tacgcatata tttatctccg caaggcttaa aatttcgttg gccttctttt ctcaaaaatg
      901 ccccgatttc gccttttgat ggatagattt ctgcctcagc aaaaacgtct aaaatatcat
      961 cagtggtcat agacaaggcg atttttaatt tttttagcaa cacattgttg atatatttga
     1021 tttccttgct tttttgcggc gtgtctacta tcggcttatc tttttgcgga ccacgcgcca
     1081 agagaatcag gccatttagg aatgcgtcaa aaacttggtt gttgatggtt ttttcataca
     1141 cattattttc tgcattatct ttgttcttat cctgtacttt ggtcagtacc gcctgagctt
     1201 cttctttagt cagtggtaca ccgcctagct caaaagcttt aatcaaatcc gtatctttca
     1261 aatcaatcgc ataacgcaat cgcaataacc tatcattgtt attcatttgt tcctccgaag
     1321 tatttagtct ttactttctt acgtactttt tgtcttgcta agatttttag gatcaatgat
     1381 aaaaaccgca tcacttatga taaggcgctg ggtatcatga gtgaatgata ttgctgaata
     1441 caaatagtcc gaccacatta agttgatcgg acttcataag cattttattg actaatactt
     1501 ttttgcttaa gaattgattc taacatcttt tgctaaagtt aagcatccta tagttcctgc
     1561 attatctgct aacttaggtg tgacaatgta ttcttcgata tttggcgtat cgacatagcc
     1621 gttaattagt tctaaaaaac gtctttgtac tttctttttc agatgttctt gttgcatgac
     1681 accaccacca aaaattataa tatctggtga aagcatcagg gtcgtattac aggcacattg
     1741 tgcaatataa tccgcctcga tttcccagaa cggatcttct atagaaagat cttgtccctt
     1801 ggttcccgta cgcatctcga tagcgggtcc agatgccaac ccttcgagac aatcatgatg
     1861 aaaaggacaa gtaccagaga aagtatctcc tgaatgtctc ttaactaacg tatgccccat
     1921 ttctggatgg ctaaaccctt caacaaaaat cccatcttgt atagctcctc caccaattcc
     1981 agttccaaca gtgtaataaa caacactgga taaacctttt ccatgacctg aaacatactc
     2041 cccgtaacaa gcagcattca catcggttgt ccaggcaatt ggaatttcga aatgcttatt
     2101 caacgttcca acaaaatcaa aatttttcca ggcagttttt ggagtagagg tgatatgtcc
     2161 ataggtcttt gattcacgat ggatatcaat cggtccaaat gaccctactc caatgctttc
     2221 taattgttct ttatagtgat taaagaaatc aattaccaaa ctcattgttt cattcggtgt
     2281 agttgtagga aaactcactc tttctaaaac ctttaaatca tcattgccaa tggcacaaac
     2341 aaatttagtc ccacctgctt ctattgagcc atacatttct tactccaccg tccttttatc
     2401 taggaattcg ttcttccctg tctaatagtt tttcgaaaaa ctcatgtggt tccttttggt
     2461 accaataggc aacagaagaa atatcgtctt gacgctcata aagcttgatg tcatcatttc
     2521 ctagggtttg gaaagtaaca cgtaaatttt ctttgaaact aatggggtcg ggtaaatgcc
     2581 agcggtacaa cccatgcatc ggtaaagcat catcgccaaa accatgaact ggatttggat
     2641 taggttttcc agcactaaaa taatcccggg tgtgatctct tttagattga aaaggatagc
     2701 ccataaatag cgtattaaaa ttttgagcat atgccctttc tccaaagttc tttttatgga
     2761 aagcccaagc gccgccaaaa taatcttctg ctcctgtaga tgttattgta gggaaatctt
     2821 catctccatc tatataaaac ttaaattctc cttcccccca ccagtatctt tccaatgcgc
     2881 acaaagcaaa gtaggttcct acataatacc cagtgccttt gattccatca ataagggtgt
     2941 aatcttttcc ttttgtcgtg atgggttcac ggttccattt agcatgaaaa tacatcgcat
     3001 catccggtac ttcagtcaat gcataattaa atgtataaaa aatgtgctta ataaactcag
     3061 gatgttcatt cgttaaagta atcttagctt tttttcgaaa tggcatctca aaatagcaat
     3121 tcatccctga tgttggattc actacgattg gtaaggaatt cacttcgcat cttgtcccaa
     3181 acccattaca aaaaaaatct cctaatggac tttctacaga aggagtggat tcatcatccc
     3241 aataaatacg caaaattaaa tcacgtaaga cgaaactccc agcgattgtt ttgtctgtca
     3301 ctgtcatcca aaaatgacgt aaaattccag ggccttccat ttctgctaaa gtaattgtct
     3361 ctccagcatt tatcggaaca gaaggtgacc cttttctccc aattcctaga tgactcgctg
     3421 tcattgccgc tttccctttt tcaccatttt tattttctgg tgaaatagtt ctactttcct
     3481 ctcccttgtg gagccatata ttatttaaac aattcatttt attcgtcctc tatcctttct
     3541 tcactttttt gattattcat atagatgatt tgttgttgtg cctccgcaat aattgctcca
     3601 atatctaaat tatcatttcc taactgcaaa gcgattgaca ttgccaaggg aagattcatt
     3661 cctgcaatta cgtgcactct gtcagaaata taggtaaaaa acttttgatt tacacttcct
     3721 ccagccatgt cagttaaaac aattatttcg tctgtagtat ccactaactc tttaatttgt
     3781 tcatctatat catcaatttc ttcgacatat gcacataagc tactaatttg attatgcata
     3841 cctgttaaaa actctagtgt ctctttaaaa ccctcagcta gtattgaatg gctagcaatg
     3901 atcaatcttc tcataattca aatccttttc ctttacgcta atattccaaa tgcagccata
     3961 accatcgcaa aaacaataac gagtagaatc aaaatggtca tactgatttt tttcttactg
     4021 agtagataat atgctgcagc agtaataatt actggaatta atgttggtaa aattttgtcc
     4081 aacattggtt ggattttcat actaacttca ccaaaagtga atttaagtgg cgttgaaaca
     4141 ctgataactg aagcaattaa tgatccaact actgttagcc ccaatactga agcagcttca
     4201 gttaaataat taatcttttc accgtattca gtaacaatct tcattccctg cttataacca
     4261 aattcaaagc ctctcattcg gaaaaagaag attaccatga ttaaaatgaa ccataaaaag
     4321 attcctacag gattattttc taaagccata taagccgcaa ttgatcccat gatggttggg
     4381 atcatcgtca tcaaaagaga gtccccaata ccagcaattg gacccatcgt accaacttta
     4441 aagtcattaa tcgcatcttt tgcttctaat ccttgctttt cctccattgc aacacaagca
     4501 cctaaaataa tggctgccag ccaaggctgt gtattgtaat actgaaagtg attatttaaa
     4561 gcttcgatgt attcatcatc atctttgtat aattttctta aaataggtga caatgcgtaa
     4621 gtcacagatg caccttgctg tgtttgataa ttaaaggtac aaactgacat caaccaacga
     4681 tagttcgctt tcctaagatc ctttttcgtt acttgatatt tttcgctcat tttattcatc
     4741 ctccccaata aacccaacat cagaagctcc cgcagaagca gtgatttgtg catctttttc
     4801 agctctatga tacacattaa atgctaaagc gaatcctatg atcgccgctc caataatagg
     4861 taccttcaaa aaagcggcta agaaaaatcc aacaagtaaa taactaatgt atcgacctgt
     4921 aggcataaag cgtaacaaca tcgcaatacc aacagcaggc aacatttttc cggctaatgt
     4981 tagcccatcc ataaaccaat taggtaaaac atccaaaacg taattaacag cagactgtcc
     5041 taaagtaacg gcaataaata ctggaattgc tgcagataaa ccaaagaaaa ttggccccaa
     5101 ccacaaaaca ctattcattt tcttaaattc tcgtttattc gcatattcct gtgatttgcg
     5161 aacaacaaaa ccatttaaga ttttgacaat tacatctagt tgcaccccta acatacctac
     5221 aggtagtcct attgctaatc cggcttctac tccaagctgg ctaccaaatg ccgttgcaat
     5281 aattgcgcac gttccatagt ccggtacaga tgagccacca aaaccggcaa ctcctaatga
     5341 catcaattga actgtaccgc caactatcaa acctgttttt atatcaccca tcaaaattcc
     5401 tgtgaataat ccccaaaaaa ctgcgtaatt tacaaagatc atcggaatcc cataataatc
     5461 aacatgttta ataaatgcta atatagtcag cataataatt tgaaaaatag taaaatgcat
     5521 tatccttact cctttatcct aattttgatt gaatttcctg tgccttatcc gccggaacaa
     5581 attgtgtaac aatttctaca ccagaatcca acaatttttt atacgtgctg atattttcat
     5641 ctgatgcata ggctctagaa gatagctgta atgcattatc tttaacaaca gtaccgccaa
     5701 caattagtcg gtcaattcta ataccgttat ctactaaatg aaggattgtt tccggctctt
     5761 tcacgatgac aaatactttt tcctgatcgt atttcccctt tttaaaattc gcaatcgctg
     5821 tttcttcagt gataattgat acagccattc cagcaggtct agctaaacgc atacttgatt
     5881 tttggatgtc gttatttgcg acagaatcat cgatcaccat cactctgttt gctttagaca
     5941 ttggtgccca ttgcgtgaca ataatcccat gcaataaacg gttatcaact ctcgacataa
     6001 caatactcat aaatacttcc tcctttatct ttcattttct tacaagcaat taccatgcca
     6061 aaacaaaaag aacttgataa atcaagctct ttttacataa tcataaacgt atttaacctc
     6121 atgaatcggg atgtcgacac tataataatg ttcaacgaca ctgaaagatt gcttaaaatg
     6181 tgtctgaaat ttttgaaatt cctcactcgt ttcattgaaa taaatttcat cttccaaagt
     6241 attttgtttt actaatcgtt caattaagca actaatgtga acatataatc cgaaacacgt
     6301 attattatca aatgaaattc ctagatcatt ttgtaagatg tatatagcat ctgaaacccg
     6361 ttctaataat ttagtcggat ttaatatggt caattgctcc attaaattcg ttagactaaa
     6421 attcttcaga atattattgt
//
LOCUS       DAEGIQ010000131         6322 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000131 DAEGIQ010000000
VERSION     DAEGIQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.135"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            59..478
                     /locus_tag="KMT45_002516"
     CDS             59..478
                     /locus_tag="KMT45_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBE7005950.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            490..960
                     /locus_tag="KMT45_002517"
     CDS             490..960
                     /locus_tag="KMT45_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7005951.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            977..1756
                     /locus_tag="KMT45_002518"
     CDS             977..1756
                     /locus_tag="KMT45_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7005952.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            1743..2564
                     /locus_tag="KMT45_002519"
     CDS             1743..2564
                     /locus_tag="KMT45_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE7005953.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQIIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            2581..3630
                     /locus_tag="KMT45_002520"
     CDS             2581..3630
                     /locus_tag="KMT45_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBE7005954.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            3643..4647
                     /locus_tag="KMT45_002521"
     CDS             3643..4647
                     /locus_tag="KMT45_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBE7005955.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            5280..6299
                     /locus_tag="KMT45_002522"
     CDS             5280..6299
                     /locus_tag="KMT45_002522"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBE7005956.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         2081 a         1024 c         1382 g         1835 t
ORIGIN      
        1 aattttggca tgcaacttgc ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt
       61 ggatagagaa attatcttag cttcccatgg aaaatttgct tcagggatat tagattcatt
      121 agagctgatc tatgggaaaa atcattctat tactgtattc gattgctata ctgatgagaa
      181 ttttgattta gcggaaacag taaggcagct ttttatgagg tacaaagata aagaaatcat
      241 tgttctgacg gatctttttg gcggaagtgt caacaacgaa ttcttacaat atatcaatcg
      301 ggatcatgtc tatctcgttg ccggattgaa tttgccacta ttgattgagc tagtatctcg
      361 gttggacgct gaagtagaga caactgtttt gatccagcag gtgctaactg attcaaaaaa
      421 aatgatccag ttttgtaacg agcggctcaa tcagaaaata gaagaagacg aattttagga
      481 ggaagaaaaa tgattgtatt gacaagagtc gaccatcggt tattacacgg acaagtagct
      541 ttttcatgga cacaaacaat aggcgcagac tgtattttga ttgctaatga tgatgtacct
      601 acgaatgaga tacgaaagac aacgatcaaa ttagccaagc cacaaggagt caaactagtg
      661 atcaaatcaa ttgatgactc gattgccgcg ttgaaaagcg gggtgacaga taaatataag
      721 ttattcatcg tagtggaatc gatcgaggat gcatataagc tggcagaagc gtatccagaa
      781 atcaaaacca tcaacttagg agggatcaag gcaaaacctg gtactcgcag catcggaaaa
      841 gcggtaaata ttttaccaga agaggaggaa cgcttgaaac gattaattga caaacaaata
      901 gaagtagaaa tccgtcaagt accagcagat aaaaaagtgg atgtaagaga agttttgtaa
      961 gaaaggaaga acctcaatga ttgtacaagc agtcttatta ggaattgttg catttgtcgc
     1021 acaatcagaa tatgcactag ggacatcgtt gctttctcgt ccgattgtga cggggctgtt
     1081 tacaggaatc gtgttaggtg atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt
     1141 agcatttatc ggatcgttct cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg
     1201 gattttaggg acggcttttg ctattacagc tggagccggt acagaaaccg cgcttttact
     1261 aggtttgccg attgctacgt tgacattgat attgaaaaat atttacttag ggcttttgat
     1321 tccaattatg aaccataaag cagacaatta tgcagaagaa ggaaattata aaggagtcga
     1381 gcatatgcac ttgcttgccg gtatcggtct ttccttgatg cttggtgttg tggtaatggt
     1441 ttcttatatg gtgggaagca atacgatcgg aaatctatta aatatgattc ctgacttcgt
     1501 tcaaaagggg ttgtcagtag ctacagggtt gattccagct cttggttttg cgatgcttgc
     1561 tcgtttgctg atcaacaaac aagttgcacc atattttttc ttaggttttg ctatagcttc
     1621 ttacttagag attcctgtga ccggagtagc tatttttggt gcgattttag cagttgtggt
     1681 agtcaatatt atgaatgtcc gtcaactacc acttcaaaca actgcagggg aggtagaaga
     1741 agatgaagaa ttctgagatc aaaaacagca aaataacgaa aaaagagcta aatcacgtat
     1801 tttggcgttc ttttcaaatg gagttttctt ggaattatga acgtcagatg aatatggctt
     1861 atgtttatgc aatgattccg attttgaaaa agttatatca gtcgaaagaa gaaatggcag
     1921 cagctttgaa acggcatcta gagtttttta acacgacccc acatatcgtt acactgattt
     1981 taggaatcac ggctgcaatg gaggaagaaa ataaggatga tcctgaattt gatgtgactg
     2041 cgatcgatag tatcaaaaca tctttgatgg gacctcttgc agggttagga gattctttct
     2101 tttggggaac tttgcgattg attgccacag gtgtgggtac ctctttagcc atgcaaggaa
     2161 atattttagg ccctatcttg ttcattttga ttttcaatat tccgcacatt ctgtttcgct
     2221 atctagcaac aggttgggga tatcgcttag gaacgggttt tttgaaaaag atccaggcaa
     2281 acggtatgat ggaaagtttg acattaggcg cgtctattat tggactgatg gtcgttggag
     2341 caatgactgc cacgatgatc accatcaata ttcctttaaa aatcggttca ggagaaaatg
     2401 ctgtgactgt tcaaattatt tttgatgata tcgttccaaa tatcttatgt cttggagcat
     2461 ttggcatagt gttttatcta ttgaagaaag aagtgaaacc attaacgatc ttgatcggac
     2521 tagctttctt tggaattttt ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa
     2581 atgtttacga tgcaagatta tatctatgaa gaaaaggaag tactaagtac gatcttgaaa
     2641 aagaatgatt tttcgacaag agaacatatg aaaaaaacag tgaatttact gatcttagca
     2701 acgggttcat cttataacgc ttgtttagca gcgaagccag cattggaatc ttatggtgat
     2761 cttactgtcg atattcaaga acctttttat tttttcaatt atggaaaact ttctccttca
     2821 atcgatactg tgattgctgt gtcacaaagc gggaaaagtg cttctactgt tgaagcagta
     2881 aaaatgatac aaaaacaagg gttacctgtt gttgctatca caaatgacgt gcaaagtcca
     2941 cttgctttag aagctgcaca aattattgat ttaggtgccg gggtagaatc agtaggcttc
     3001 gttacaaaag gctattctgc gactgtttta cagttactac tcttagggat aggaattgga
     3061 ataagtaaaa ataaaatcag caaaaaaata gaacaagact acatgcaaca gttgcgcaaa
     3121 ataatcaacc atttaccagc gattattcaa aaaacggaag aattttttga tggatatcaa
     3181 agtttatttc gtttagcaca gcgtttcgtc accattggat atggtccaaa ctggggaaca
     3241 gctaaagaag cagagacgaa attgactgaa acaatccgcg ttccgtcaca aggatttgag
     3301 ttagaagctt atatgcacgg cccatacctg gaggcagatg cttcgcattt gctattcttt
     3361 attgaagggg aaagtgtaaa taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg
     3421 tacgttggag agactctcac cattaccaca aaaaaagcaa gaaacgagaa aacacttgga
     3481 ttagcaatag aatgtgatga atatctgtct gtattagcgc ttgtcgttcc gtttcaacta
     3541 ttcgcttata aaaccgcagt agctaaaggt attgacttga acaaaaagat ttttgaagac
     3601 tttgatacag tcttgaaaag taaattataa gaggtggaaa tcatgctgaa attcaatgaa
     3661 gaagaacaaa tcaaagcaat aaaaggcgca ctagctttac gtccgcaagt agaagaaatc
     3721 atcgataaac tttatgcgga aaaatttgac gctgtctatt atctaggaat cggtggaacg
     3781 tatgcttcct cgatgcaagc tgtcacttat atgaatggga aaagcaactt gcctgtcttt
     3841 gtacagcatg ctgcagaata ttacactact ggaaacaaaa gattaacaaa agattctatc
     3901 gttattttgt cctctgttac cggtacaact caagaagtag tgaaagcagt agaacaaatc
     3961 aagaaagtag gagcgacact gattggattt atcgataaag cgaacagtaa gttgtctcag
     4021 ctatgcgatt ttgtggtcac ttatccagct cctggaacag aacaaatcaa attttttatg
     4081 gcagcagatc gattgatgta tttgaatgga gaattcgaag cttattcaga atactatgaa
     4141 cagctcgaac gttatttacc aacagggtta gtggaagcag agaaaaaagc agatgcattt
     4201 ggcttatcat ttgcagaaaa acatcgacgt gatagtatgc attattttat tggagcaggg
     4261 aatcaatggg gagctgttta ttcatatgcc atgtgttact gggaagaaca aagctggctt
     4321 ccatcgaaat ctatccatgc tgccgagttt cttcacggga cgttagagat cgtagaagaa
     4381 acaaccccag tcacattatt tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg
     4441 gctaaacttt tgccaaaaat ttgcagcaat tatacattga tagacacaaa agactatcca
     4501 gtagaaggta tcagtgaaaa atatcgcgga cgtgttttgt ctttcttatt gatgcatgta
     4561 gtgacacaac gcatcgatgc tcatgtggag cagttgaact gccatccatt agagatacgc
     4621 cgctactatc gtcagtttga ttattaggat agactggata aaaagaatat aaaaaataaa
     4681 gagagcagcg ataactgctc cctttatttt tttaaaatat tggcatcaat tacgttataa
     4741 aacacatttt ttgcattatc tacagttcaa cttataatct gtttaatggg acatgtgggt
     4801 gccaaccatt ttttgttatg caatagtact atttagttgt tgcatggatc cgtagtaaaa
     4861 gtccaagtac aacgagttgc gccagtttac atagcgtttt gtatccagaa tgttgactaa
     4921 aacaaaagtt cctatgtcaa ccgtcaacag agacaatctg tccgttgcag gacctgacag
     4981 atttagaaat gttcaatctg tctcttatgt attttaaatc aatactcact aggagaagtt
     5041 tcctaacctc ctaatagtaa tgctggtagt aacattgtca aaactgcttc ctcttaggaa
     5101 caagcaaagc ataacgagtg taaaaataaa atgtagacta ttgtaacata tcattgatta
     5161 tttcaagtaa aaacgataaa agaagtaaac aaacacttaa aaacaatgga aattagtccc
     5221 aactttaaga aatgtatact ataatggaaa ttatcaatta tttatttgga ggaatatcca
     5281 tgaaacaaaa aatcgcagtt cttggtcctg gatcttgggg cactgcttta gcacaaacat
     5341 tggcagaaaa cgggcatgat gtccgtattt ggggaaatgt acctgaacag attgatgaaa
     5401 tcaataagta tcatacaaat cagcattttt tacctgattt gacgatccca gaatcgatta
     5461 tcggatataa agaattggca gaagcagtgg ataacgcaga tgctatttta tttgttgttc
     5521 caacaaaagc gattcgttca gtcgcacagg aacttattgg taaaatgaat actaagccaa
     5581 tcattataca tgcaagtaaa ggactagaac aagatacgca taaacgaatc tcagaagttt
     5641 tggctgaaga aattccagaa gaaaaaagac aggcaattgt tgtattatct ggtcctagcc
     5701 atgctgagga agtggcagta catgatatca caacaattac agctgcaagt attgatcaaa
     5761 aagcagcagc ctatgtacaa aaactgttca tgaatgatta tttcaggatt tatacaaata
     5821 atgatgtgat tggcgtagaa actggtgcag cactgaaaaa tatcattgca attggtgccg
     5881 gcgctatcca tggattagga ttcggagatg atgcaaaagc agcaatcatg actcgaggat
     5941 tggcagaaat cagccgtctt ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt
     6001 tgagtggtgt gggggacttg atcgttactt gtacaagtgt tcattctcgc aattggcgtg
     6061 ccggtaactt gttaggacag ggacataagc tggaagaagt tctagaaaac atgggaatgg
     6121 tcgttgaggg tgtggcaacg acaaaagcag cagtagagct agctcaacag ctaggtgttg
     6181 agatgccaat tactcaaaca atctataatg ttttgtataa tggagaagat atcaaaaaag
     6241 cagcaaaaga aatcatgtta cgtgacggaa aaatggagaa tgaattttct cttcgttaaa
     6301 tagatgatca atagttaatc gt
//
LOCUS       DAEGIQ010000132         6320 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000132 DAEGIQ010000000
VERSION     DAEGIQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.136"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(98..1228)
                     /locus_tag="KMT45_002523"
     CDS             complement(98..1228)
                     /locus_tag="KMT45_002523"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBE7005957.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="KMT45_002524"
     CDS             complement(1877..3079)
                     /locus_tag="KMT45_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE7005958.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="KMT45_002525"
     CDS             complement(3139..3342)
                     /locus_tag="KMT45_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBE7005959.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="KMT45_002526"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="KMT45_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="KMT45_002527"
     CDS             4879..5691
                     /locus_tag="KMT45_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005960.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1878 a         1299 c          996 g         2147 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa
//
LOCUS       DAEGIQ010000133         6170 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000133 DAEGIQ010000000
VERSION     DAEGIQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.137"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            153..1784
                     /locus_tag="KMT45_002528"
     CDS             153..1784
                     /locus_tag="KMT45_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBE7005961.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1787..2866
                     /gene="uxuA"
                     /locus_tag="KMT45_002529"
     CDS             1787..2866
                     /gene="uxuA"
                     /locus_tag="KMT45_002529"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBE7005962.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2940..4367
                     /gene="uxaC"
                     /locus_tag="KMT45_002530"
     CDS             2940..4367
                     /gene="uxaC"
                     /locus_tag="KMT45_002530"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBE7005963.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4451..5098
                     /locus_tag="KMT45_002531"
     CDS             4451..5098
                     /locus_tag="KMT45_002531"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBE7005964.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5218..>6170
                     /locus_tag="KMT45_002532"
     CDS             5218..>6170
                     /locus_tag="KMT45_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7005965.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIV"
BASE COUNT         2109 a          831 c         1273 g         1957 t
ORIGIN      
        1 aggagtattt tatttttctg aataaaaaat acaatcattc tgatatggta gaaaaaagca
       61 agtttattcc ctttttatat tgacaacggt tacaatgggt tttaaaatat aaaatgtatc
      121 aaaactaaca tatcagtttg aggaggttaa ttatggtttc gattaaaaat tggcaaaatt
      181 ctatcgaaga attcaatgaa atgcagattc tactgccgtg ttttgatgtc aaagaagtta
      241 agcaagaagg ccagcaacat ccagtatggc ttcattttgg tggaggaaat ctttatcgag
      301 gatttcatgc agaaattgct caatgcttag cagatcaaaa tgaattagaa acaggagtag
      361 ttgtgtgtga aacatttgat gaagaagtag tggaaaaagc ttatcatgac tataacaatg
      421 acattttgga aattgttatg agtggagatg gtgaactcaa aaaaagactt ttggcagtaa
      481 cggctgatag tttatattgc aatccgaaga atatagcgga ctatcaaaga gtggttaaat
      541 attttgaaga tcctaaatta cagtttacaa cgtttactat tactgaaaaa ggttacagtt
      601 taaaagattc taaagggcaa ttcttttcgg tagtcaaagc tgatatcaaa tctggaccta
      661 gcaatcctca acatacgatg agtattgtaa catccttgtt atatagcaga tttaatgctg
      721 gagagttgcc gattgcgatg gtttctactg ataatttctc acagaatggt caacgttttc
      781 aagatagtat ttttacgatt gcagaagctt ggcaaaaaga aggatatgtt tctgaaaaat
      841 ttattgctta tttaaaggat aaagaaaaag tgacttttcc ttggagtatg attgatcgaa
      901 ttacgccaaa tccttcagag aatgtaaagg aagatctatt gatgatgggg ctcgaggatt
      961 tagatatcat tcacacaaaa aaacatacca atattgctgc tttcactaat actgaggtag
     1021 ttcattattt agttattgaa gatagttttc caaatggccg gcctaaattg gaaaaggctg
     1081 gtgtgattat gacagataga gagacggttg ataaagcaga catcatgaag gttacgacaa
     1141 gcttgaatcc acttcatact gctttggcag taacaggttg tctgctagga tatcaatcaa
     1201 tttcagcaga gatgcaggat gatgatttgg ttggtttaat caaaggaata ggttatttag
     1261 aaggtttacc agtagttgaa aatccaggca ttattgatcc aaaacaattt attgatgaag
     1321 taattaataa acgtttgcct aatccctata ttccagatac accacaacga attgcaacag
     1381 atacttctca aaaagtagcg attcgctatg gtgaaacaat taaaaagtat gtcgaaaaag
     1441 acggaaatgc agaaaatcta acatttattc ctatcgctat tgctgcttgg ttacgttatt
     1501 tactggcagt cgatgatcaa ggggaaaatt ttgtgccaag tcctgatccg ttattggtag
     1561 agttgcagga taaattatca gtattgaaat taggtgaaca agatccttca aagattcatt
     1621 gtgcggtaca ggaaatttta gaaaataaag aaatctttgg tttagattta tatcaagttg
     1681 gggtaggaaa gaaaactgaa aaaatctttt gcgaaatgct agtaggtcct caagcagttc
     1741 gtaaaacaat caataaatat ataacagaag ttggaggaaa gtaactatgg aaatgacttt
     1801 tagatggtat ggggcaaatg aagaaaaaat ctcactacaa gaaattcgtc agatcccagc
     1861 aatgaaagga attgttggaa cattaatgga tataccagcc ggagaggttt ggccgagaga
     1921 acgaattaaa tctttaaaag atgaagtcga agccaatgga cttaccttga aagttattga
     1981 gagtgttaat gttcatgatg atatcaaaat tggtttacca acacgagatc aatacattga
     2041 gaattataaa cagacgattc gcaatttggc agaatttggg attgaggtta tctgttataa
     2101 ttttatgcca gtatttgatt gggtaaaatc ggatttggat tatcgtttag atgatgggtc
     2161 ttctacgtta gcctatattt caaaaaatat ccctactgat ccggaggaaa ttattagaga
     2221 agtagaaaat tctactaata attttgaatt gccgggttgg gaacctcatc ggttatcaca
     2281 aattaagcaa ttattagaag cctataaaga agttgatgaa gagaaattac gagagaactt
     2341 tgcatatttt ctcaaagcaa ttatcccgac atgtgaagaa gttgggatta aaatggcagt
     2401 tcacccagat gatccaccat attccatttt cggattacct cgtattgtta aaaatcgtga
     2461 cgatttagat tggttgtgta atgtagtgga ttctccttct aatggtatta cattatgtac
     2521 tggaagtatt gcagaacgac cagataatga tgtctatgaa attctatctg aattttgtca
     2581 acgggatcgg attccttttg cacatgttcg taatatcaaa tatttaagtg acaatggact
     2641 cgatttttac gaagcgccgc ataaatcaga atatggttct ctagatatgt ataagatatt
     2701 aaaaacaatg tacgataatg gatttaatgg ctatattcgt ccagatcatg gtcggatgat
     2761 ttggggagaa accggtagac ctggttatgg attatatgac cgagctctag gtgcagttta
     2821 tttaaatggt atttgggaag cgcttgaaaa gggggcgagt aaataattat ataagggagt
     2881 ttatttagag gaaaaatttt ctcttctaaa aactaacgga aggaaaggag tgtttaaata
     2941 tgtttttaga taagaacttt ttattagaga atgaaatagc taaaagactt tttcataaat
     3001 ttgcaaaaca ccaaccaatt atcgactatc attgtcattt agacccagca tatttgctag
     3061 aaaataagca atacaagaat ttgactcagg tatggttaaa tgataaagga gctggagatc
     3121 attataaatg gcgattgatg cgagctaatg gtatagaaga agagttgatc agtggtccag
     3181 gtgatgatta tgaaaaattt ttagcttttg taaaaacagt ggagaaggcg ccaggtaatc
     3241 ctatttttga atggtctcat ttggaattac aacgttactt tgatattgat ttgacaattt
     3301 ctgagaagaa tgcaccagaa atttggcgac gtgctaacga aaaaattcaa acggattcct
     3361 atcgcccgaa agaattgttg gagatgatgg atgtgaaagc catctgtaca acggatgatc
     3421 ctacagatga cttgtggtat catactgagc tttctaaaca ggataattct ttcaaggtat
     3481 tacctacttt ccgtccagat aaggtctgga gtatcaaatc atcaggcttt tcgaactata
     3541 ttttaaaact tagtcaaaca gcggctatac caatcaaaga ctttaatggg ttgaagcaag
     3601 cattgagcca acgagtggat cattttcatc aaattggctg tcgattagca gatcacggtt
     3661 tgaatactta tttttatgaa atagcagatg aaaaaatact cgacaatctg ttaataaaag
     3721 ctttagcagg aaaaacggat ttccatttat cagaaattgc acaatttgtt acaggtattc
     3781 aattacattt gatgtctgag tatacaagat atggttggac gatgcaaatg catttaaatg
     3841 ctttaagaaa taattcaagt atattaaagg aacaaattgg agtagatgtc ggtggtgatt
     3901 cgatggggga tcaagctagt ttgacacaaa atgttgtagc attgttcgat accgctgaaa
     3961 agagtgggtg cttaccaaaa acaattttat attcattaaa tccaactgat tggcttccac
     4021 tagcaacagc aatgcaaagc ttccaagggg gtatgattca aaaaatccaa ttgggatgtg
     4081 cttggtggtt caatgataca gctgaaggaa tgcgtcaaca gttgactacg atggcacaac
     4141 aaagtttgtt gtcgaatttt acaggtatgt tgactgattc tcgaagtttt ctaagctatc
     4201 ctcgccatga gtattttcgt cgagtattat gtcaattact aagcgagtgg gttaaagcag
     4261 gccgaattcc agatgatgaa atgtatctta gtcagatagt ggagaatatt tgttatcgta
     4321 atgcgtataa ttattttaat ttttttgaaa tcggaagtag aaaataatac ttgaaataaa
     4381 agcttaaatt tattctttgt aagttagtac agctagacaa agtatgtatc cataaaggaa
     4441 ggagatagat atgaaaaaag tagaaatttt aagtaagtta gaagcggctg gagtcattgc
     4501 ggttgtacgt gggaaaaata aggatgaggc cattaaagca ataaattcaa ttattgcggg
     4561 tggaattaaa gggattgaac taacttttac agttcctaat gctgaagaaa taatcaaaga
     4621 aattgctaaa aaatatgctg atcaaaaaga tgtaataatc ggagccggta cggttttaga
     4681 tgcgtttact gctcgtatcg caattatggc tggggcagaa tatatcgtta gtccatcttt
     4741 tgatcgacaa acagctgaga tttgtaattt atatcaaatt ccttatttac caggttgtat
     4801 gacaattact gagataaagg aggcacttac tgctggcgta gatatcatta aactattccc
     4861 aggttctgct tttggtccat ccattgtaaa agcctatcaa gctccattac cacacgtaaa
     4921 tataatgcct actggcggag taagtataga gaacttagaa gattggtttg aagtaggagt
     4981 tgttgcagtt ggtgtaggtg ggaatttatt ggcttctgtt gaaaaaggtg attttgaaga
     5041 agtaagtcga gtagctagta aatatataga taagttttat gagataaaga attattagga
     5101 gaaaaaacaa ataatactct gaaatgtaaa aaataaatac tgactcttta cgcttcagag
     5161 tatagtacgt actttcatca gaatgaaaac gcttaacaac agaagggaga gaattgtatg
     5221 gaaagaaaat ttggtataag agacaaaata ggatatatgt ttggagattt tggaaacgat
     5281 tttactttca tttttgctag ttctttttta atgatctttt atacaaaagt attggggata
     5341 agcggagctg ccgttggaac attattttta gtagcaagat ttatcgatgc agttactgat
     5401 gtaactatgg ggcgtattgt tgatagatcg aaagcaacta agcagggtaa gtttaaaccg
     5461 tggattttaa gaatgagtgg tccagtcgct ttagcaagtt ttttaatgta tcaaacttct
     5521 atgatttcag cttctatgag cgttaaggta gtatacatgt atgttacgta tatattatgg
     5581 gggagtattt tttacacatc tataaatatt ccttatggat caatggcatc agctattact
     5641 tctattacag aagaaagaac aatgctatcg acattccgaa atgttggagc tactttagca
     5701 agtttggtca ttggtgtatt aacaccatta tttatttata caagagatga ttcaggcgct
     5761 caaatagttc gtggtggatc aacttttaca attgttgcgg gaatattttc gatatgtgca
     5821 cttatttgtt atttaatatg ttataaacta actactgaac gagtaagaat tgaccaatct
     5881 caatacaaag tttcattgtt aaaatcttta aaagggttag ttaaaaatag ggcattactt
     5941 tctataattg gtgcggctat tttcttactc cttgctcaat tattaattat gagtatgaat
     6001 aattatgtat ttcctgatta ctataatgat gcacagggaa tagttttaat gaattttatc
     6061 aatcctattt tggttttaat agttgtttcg cctttaactt tgtttttaag taaaaaatat
     6121 ggaaaaaaag aactggcatc agtaggaatg ttcttttcag caatagtata
//
LOCUS       DAEGIQ010000134         6090 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000134 DAEGIQ010000000
VERSION     DAEGIQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.138"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(254..541)
                     /locus_tag="KMT45_002533"
     CDS             complement(254..541)
                     /locus_tag="KMT45_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBE7005966.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="KMT45_002534"
     CDS             complement(561..785)
                     /locus_tag="KMT45_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBE7005967.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="KMT45_002535"
     CDS             complement(1240..1410)
                     /locus_tag="KMT45_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005968.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="KMT45_002536"
     CDS             complement(1425..1958)
                     /locus_tag="KMT45_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBE7005969.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="KMT45_002537"
     CDS             complement(1979..2923)
                     /locus_tag="KMT45_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBE7005970.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="KMT45_002538"
     CDS             3560..3646
                     /locus_tag="KMT45_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBE7005971.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="KMT45_002539"
     CDS             complement(3767..4456)
                     /locus_tag="KMT45_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005972.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="KMT45_002540"
     CDS             complement(4463..4741)
                     /locus_tag="KMT45_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005973.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="KMT45_002541"
     CDS             complement(4701..5615)
                     /locus_tag="KMT45_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBE7005974.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="KMT45_002542"
     CDS             complement(5597..5977)
                     /locus_tag="KMT45_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBE7005975.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1612 a         1125 c         1027 g         2326 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagctcac taaagggctt ggcaagctct
//
LOCUS       DAEGIQ010000135         5913 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000135 DAEGIQ010000000
VERSION     DAEGIQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.139"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(567..1070)
                     /locus_tag="KMT45_002543"
     CDS             complement(567..1070)
                     /locus_tag="KMT45_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBE7005976.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1429..2454
                     /locus_tag="KMT45_002544"
     CDS             1429..2454
                     /locus_tag="KMT45_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBE7005977.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2777..3655
                     /locus_tag="KMT45_002545"
     CDS             2777..3655
                     /locus_tag="KMT45_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBE7005978.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4131..4247
                     /locus_tag="KMT45_002546"
     CDS             4131..4247
                     /locus_tag="KMT45_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBE7005979.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5175..5513
                     /locus_tag="KMT45_002547"
     CDS             5175..5513
                     /locus_tag="KMT45_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005980.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5607..5837
                     /locus_tag="KMT45_002548"
     CDS             5607..5837
                     /locus_tag="KMT45_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005981.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2010 a          887 c         1085 g         1931 t
ORIGIN      
        1 gttttcaaac agtataaaac ggtgtggttt ttggaataaa aaaataacat atctcatttt
       61 acacttttgt tattttatac ttaaaaaata acgacacaga aaataccgtg tctcagattt
      121 ctctactact tctaaaaact actgtttttt tcctttgaaa tgccttcaaa aagtaggatc
      181 aggcagaata tactggcaga atcaaggaga agggagccta aataaggaga aaaaccatag
      241 aaaatataaa gtaattactt ataaaacaag ctttttatag aaaaataata tacctgagta
      301 gggtataaat taagacggaa ttcctttcta gattgagata ttgactagga agaatgttaa
      361 gattttaagc ctttaagcta aaaaaaaacc ctttttgtta aataagggga atatagcctg
      421 tcagtttcaa actgcaagtc gtcctgctgc aataatatag tagattagag atgaaaatag
      481 atttttttaa accaatgact gaataaaaag accgtcatag atacacattg gaatgtgtct
      541 attacggtct ttttttacga acgattttat tgaagacgga cagttcgggt caaaaataaa
      601 aaagctacga tcagaaataa gaataaaatg tatcccaaaa taatggctag acggtctaac
      661 ttatttgtcg atagatgttt gtttagaagt tgataggccc ataaacctat cagtcctcct
      721 gttgtattag ttatcaaatc agtgatgtcc gctacgccaa taccgaatag gaattgaatc
      781 aattctgcca agaagcttac aaggaaaata atccctaatt ttttagaaaa agaaagtctc
      841 ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga caatcaagtt ataagtggtc
      901 tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa aagggacgag attgacattg
      961 ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata gcttgaataa aatcaaccat
     1021 aatagaaaga ataaataaat ccctaataaa aattttgata cataccgcat agacatctcc
     1081 tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc ataaaaaagt atgaagttta
     1141 aggaccaacg tagttatcgt acgaaaatta gaagcttttt tttgttggga atccggtttc
     1201 tttaacaaag ctaagatagc gagttgcttg gaaaagttgc tatctttttt ctgtcaaaac
     1261 gactcggaat gttcgttttt tgttctaatt tctccaagtt gtttactttt tgatactaag
     1321 tttatgaaaa atcaagaaat agttgttata ctaggtaatg ctgacaaacg ggattggcat
     1381 ttcatcttgg ttcagctttt tttccaatta ctaggaagga gggatagatt gttcaagact
     1441 tattttcgtt tcaataaaat acaagataat ccttttcgta tcatgaatct gatgctactt
     1501 atctttattg tattatggtt aggttacata aatcatgatt ttttgatttc aagtttttca
     1561 tcattaggga tctttacttt tttctattat caaaacatac cgatgaaata tttgctgaaa
     1621 cgtatgtttc tgatcggttg cggactgttg gttgctttcg ttttggggat cttatcgact
     1681 tatgttttat ggcttgaacc atttgccgta gctttggttg ctttttccag cagattcgtg
     1741 ttgcgattat tccatatttc taaaccaggt ggcttgtttt ttgcgatgct ttctgccatg
     1801 ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg tctcacttta tttttttatg
     1861 ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat tactggattc acggcctgag
     1921 cagacaattg aaaaagcaac tttaaaagaa agatttcacg aagagccttt agtgatcatt
     1981 gattctgttt tctacagcgc tgccttattt ctttccgttt atgtcagtca cggcttgaat
     2041 ctgcataatc cttattggtt gaccttgtca tgtgcatcga ttttgctagc agaaaacttg
     2101 gatgcgatga agcatcgaca agtccaatat ctgattggat cgatgggtgg gttatgtgtg
     2161 tcggcattct tgtcatttgt gccgtttacg cagttgcaga ccatttttct gatcactttt
     2221 ctatatggaa tcgcacaatt tctagttgct agaaattatg cagttgctaa tatttttctt
     2281 aatccgatgg cgttgatgct atctacactt attcgtggag catacttgat ttctttgatc
     2341 gaatatcgtt ttttagggat cgtcatagga agtttcatcg gtttaggtgt tgcgtgggta
     2401 atgacggttg gtttgcaaca ttatttaacg gttgttcgtc aaaaattaga atagaaggac
     2461 aaatatatgt gccaaaagag gttatgacaa gatttttgtc acaacctcta attcaaggtg
     2521 ttcaaaagcc agattctcag cattaagtca aattcacaca aaaatgtgga aaggaacttt
     2581 caaaaaaatt ttatatttat tctatcaaaa aattttgttt atgatggtaa ctgaataaac
     2641 tttcctgaga tggtcaatgc tttccttgaa agtcttacag atattctttc ttcatagaaa
     2701 gtttaagttt tatagttagt gtacaataaa agtatgttta taccatatgg tgtccggata
     2761 atcagtcatt taaacaatga ggttcaaaga tatgaaaagg ggaaatttga tgaagaaaat
     2821 aagcaatgaa atccaaattc tggcagaaac tttaactctt cccaaagtcg ttatagagca
     2881 aatgattgta caagcaaaaa acatccttga tgaggtgttt gagcaatata gtcatgaact
     2941 aatggatcct aataaagcaa aggcgactgc aaagaaaata gacaaagtct atgaagagga
     3001 accttttgca gcattagcta tttatctata tgcagcttct caaagttgga aaaaaattta
     3061 tcaaccattg aagattccaa gaactatcta tttggcaaca atgaatgctt ttactagatt
     3121 tattcatgaa catttccaag taacaggaag ctatcgcttt gaccgtggtt tttggatttg
     3181 gcattacact agtggcttga tctttcggat tggtgaattg gagtacgaaa tgacatattt
     3241 tccgaaaggg tacacggatc taagattaga aggaaagcag tgcctttctc ttcatattcc
     3301 ttccgatgca gatttatctc agaataagat aagagatagt tacgaaagtg ccattcgttt
     3361 ttttgaactt tattttcctg attaccatta ccaagtgatg tacacagata cttggttact
     3421 atcacccaat cttactaaat ggttgaaaaa agagtcaaaa atctgtcttt ttgcagccga
     3481 ttatcgatta ttatcagttg atgaacagga cgatagtgga gtcccttgga tctttggacg
     3541 agtagatgcc caaatacatg attatccaga aagtacgagt ttacagcgac aagcaaaaga
     3601 gcaattgctt gcaggcgagc acattggctc tggcttaggt attcttactt cataagttta
     3661 gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa agaacacttt tgtctcacgc
     3721 tctaaacacg tataaacggc ggaagcagaa gcaactcctt cggaaataag ctgaaattca
     3781 caaaaatttg aaaagcaatt ttcgtgaatt tcttcttgtt tcttggagtt aaacgcttct
     3841 gtcccagcct cttttttagt gttctatcga acacacagta cgttcaatag gtaaactagc
     3901 tataacaata gatattaaag ttaacggaca agagggcctt ttttaggtag ctaaaacgta
     3961 ataatgaaaa tttatttcca gagataggag tcaatcagct agtctgatcg actgttttaa
     4021 caagttgact ttcattttaa cttttgatac catttaaaac acaaatcgta attgtttcga
     4081 tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg ggtcgtccat atggaaaaaa
     4141 cagatatctc aagtgcatac cgccgcttga aaagcccaaa tatcaaaaca cgaaaacgtg
     4201 cgctgaagat catcaaagat gtgaaacgga atagtgggaa aaggtaagaa gaaggaaact
     4261 taattaaaaa aatagccgaa aaacgttttt cggctatttt caataatcgg tttttgtgta
     4321 aggtttccga aagaactgct gttgattttc catttttaac atatgataaa ttggtagaat
     4381 ctttcttttc aaattgatac cattccctaa ttccagttat atagttaact acagaaccac
     4441 aaagcacgta cgtttataaa agtattatct gtagttattt ttcaattccc tttcaacctc
     4501 ttattttgac cagtagtatt gatcacttta ccttttaaca cctttgttcg tttttgaaac
     4561 atttttagtc aagaatggaa acgtaactac ataatcaaag gttttgcgaa atatttcatg
     4621 aaatatttca tgtagaagcg agcaatacgg ttgcaaataa ccattatcgt ttaataagca
     4681 gttacgaaag ctcccaatat ctctaacgtt cagctcactc catttctttt cttcaaaaaa
     4741 atcagtgatt cgtatctctt tctatttgtc gaaaaagtta actttaaaaa gtaacgccga
     4801 aaaaatatgc aaaaaaataa aaaagcagtt actattgcta gaataacatc aaggaataat
     4861 aagttttggt tttcagataa tagtcgataa gttgtgtatt ggaattttag atacagtaat
     4921 aaagtatttt tcatgaaatt ttgtgagaat cactattaca agtatcttta ataaaattaa
     4981 tggttaagga ggaacttaaa gtagaatgta aatgaatagt tgtagtcagt cgattgtgat
     5041 actagactca aagttgcagg aataagtttt tcaatttgtg attggaagat aaaaaatgaa
     5101 aaagaagggc ttttttttat actttgttga ttgtagtgtt tattttccct ctgtttagcc
     5161 aagtaacatt ttctatgact caaccagttt caagtgagga acatgataac ctttttcgtg
     5221 ctacagaaga gtttaagaaa tatcaggaaa atattgttcc ggagactcca ttagctagtg
     5281 agccaattac tgatattgat ggaaaccaaa taggatggta tatacagtac gaagttatgg
     5341 tggtaaataa tgactcaata gtaaaaaata cagtaggctt agagctaaca ccgattgaaa
     5401 aggtgagttc attgttaacg tatatttata ctgatgaaag tgacgagact gagatcattt
     5461 taacggatta ctcaaaaatt gctactgata aaaagtatat attgtggatg tagacactag
     5521 tgaaatactt tatgatatag aaattgatga aaatttacta gatgtagcac aagaaatcga
     5581 tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa gggaaaagaa aatatttatt
     5641 acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg gaaagaattc aagagtcgct
     5701 actcaattaa ctcgactccg acatttacag tttacagaga aggatcgata gaaaaaactg
     5761 ttttttggac taaagaaagt ggaatgtctt tagctgaagt agaagagttt ttggattatg
     5821 ttagtatgca acagtaaggg agttcttatt atgaaaaatt ttgacacata ttatgttatt
     5881 gttttttcga tatctttgct gttaatatta cta
//
LOCUS       DAEGIQ010000136         5818 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000136 DAEGIQ010000000
VERSION     DAEGIQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.142"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            365..682
                     /locus_tag="KMT45_002549"
     CDS             365..682
                     /locus_tag="KMT45_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010624545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7005982.1"
                     /translation="MTTRILLVCGAGASSGFMAAAARKAAKKNGNEFEIKAKSESELD
                     NNLDTTDLLLVAPHLKYMIDDVKDTCEQKGVKYGIIPQRVYGALDGNGLVKFAEEILK
                     EGN"
     gene            684..1010
                     /locus_tag="KMT45_002550"
     CDS             684..1010
                     /locus_tag="KMT45_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003135859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBE7005983.1"
                     /translation="MENVEKDELNLLSMNVILHAGNARDYVFQAVERASDGDFTESDK
                     LMKQANDEVVEAHRAQTSTLQKEADGVEIPYSPLFGHAQDTLMTVKTEINLMKEIIKI
                     YRNLKG"
     gene            1016..2260
                     /locus_tag="KMT45_002551"
     CDS             1016..2260
                     /locus_tag="KMT45_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7005984.1"
                     /translation="MDKLMTWMTDKFAPKVNKIAKNTWVASIQEAILTAMPMVFIGSF
                     ATILSIVKNYWSSFPDFSLLSTFSFGLFSIFLAFLIPQAVMKNKKIAGVEKQAGLAGV
                     AFFLLVIFPSISADGGNITFDLNAFGTGGMIAALVSGMFVGLIMSLFSKLKLIDEDSP
                     IPDFVAVWFNTLIPIISILLVGWLFTFQLKINLYELINSLFLPLINLGSGFWGFVIIS
                     FLAYSFLYTFGISSWVLYPVQVAITLPAIAQNQANHAAGKAVTNIFTSEVGQLFLIGG
                     GGATLSLCIMLAFLAKSKRLRMIGKASLIPSIFNINEPIVFGAPIAFNPILMVPMWIN
                     GLVGPIVTWFAMKSGLVPIPYEPFQLWYLPAPIIGWITTKSIMGILLVVILFILSWLI
                     YYPFFKAYDKQAVEQDLELEGE"
     gene            2267..3718
                     /locus_tag="KMT45_002552"
     CDS             2267..3718
                     /locus_tag="KMT45_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004166581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBE7005985.1"
                     /translation="MKTNSYPYFPKKFLWGGAIAANQAEGAFDVDGKKPNTSDVQPYL
                     KGLSNEEIQRVEMQGMTLKQVNHNLKDTTQFYPKRYGIDFYHTFESDLELLAEMGFKT
                     FRTSIDWSRIFPNGDEIEPNERGLAYYDKLINKIISLGMEPIITMLHYETPINVTLSF
                     GGWQNKKVVDMFERYGKVLLDRYGNKVKYWIVINQINMIQVEPFLSLAICSDQFEKTE
                     EAEYQAVHNQMVASAKIRKYAAGLPYDLQIGTMVADGTVYPYSGHPDDVVLAMRHNRM
                     QYFFTDVQFNGEYPQYARNYFKENDIFLDITPEEIELLRENTMDYLAISYYFSQMVDS
                     KQNVDKPGSTTSNPNLEQSPWGWKIDPQGLYNSLSQYWDRYHKSIIIAENGFGMYDEL
                     EDGKVHDDYRIDYLSRHIEQVGRAIYDGADVMAYCAWGPIDIVSCSSQQMSKRYGFIY
                     VDIDDDGKGSGKRFKKDSFDWYKEVIASNGKYI"
     gene            3791..5710
                     /locus_tag="KMT45_002553"
     CDS             3791..5710
                     /locus_tag="KMT45_002553"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012579.1,HMM:NF013070.1,HMM:NF016901.1,
                     HMM:NF019884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBE7005986.1"
                     /translation="MHNKERLIEYLKKFSDDRFVPAKELAEVLDVTDRTIRNYIKSIN
                     TKKPDLIITSRDGYKYVAKNNYGKNLELTDQSIDSRRFYILRRLFKSSVRGIDVFDLA
                     DALYISDASVRSDIVALSRLSEKYDLVIRQKEGRYILEGNDKNKRRLMIQLIRSTNHD
                     GSTFNVDIQKFLDSIPFGEVVKITKTAFKNHNLKTNSYFLKNFVLHLAIAIDRARGIS
                     QLIEESEPHHNKKYRSYNIINEIRKSIFKEFSIKLLLEDVEELNILCEGEFRNTSFDL
                     LTFVEPKVHESVNKALGELSKIYLIDFSEKKFKNRLLLHIQSLYKRSQEKNYSRNLSL
                     MEIKVKYPVLFDMAIYLSSILSNDLKITISDEEIAFLALHIGSFVDSQKKSDKQIKTT
                     IITPSYNTHEIYIKDRIEKEFEDELKIEDVVEDILDLSNEPELIISTQSILETDRLKV
                     INIPVIIIKEFLTPHDVNQIRRKIEQIKCLRYKKFLETYLPKAIPEEFYLEIKHKVSK
                     EEVFRIIESVLVKKEYVPSNFRDMLKEREKISPTAFPSGIAVPHSIKYDAYKTGLYVI
                     NSKETVKWSNLNVNLIIALSVDKNDSEEFNKIFPRMVELLAEEINVRYLHKSLNRQEF
                     ITRMIDLMVSEDYYE"
BASE COUNT         2055 a          791 c         1095 g         1877 t
ORIGIN      
        1 atactgcttt tgaaactatt tgtactaaca tcgatattag tataatcgag tagaattttt
       61 tccataatca ctgttaaaaa tgtgttatct tttgtcgaat aactataatt tataatattt
      121 accgctggtg atatggtgca aaataaagga acaatgagcc ttatctgtcg attttcgagt
      181 taccaaggtt agttctttta cttaatggct tttattccct ctataggtta ggtaataaaa
      241 gcctctaaat aagttgaatt gaaacataca agttgactat ttttcctctt attaggaaat
      301 ttttttaaat gtatacgctt tatttagtcg ataaaataaa gataacaaaa ggaggaacta
      361 aattatgaca acacgaattc tactagtatg tggtgctgga gcatcttcag gatttatggc
      421 agcagcagct agaaaagcag caaaaaagaa tggaaatgag tttgaaatca aagctaagag
      481 tgaatctgag ttagacaata atttagatac aacagatttg cttttagttg ctccgcatct
      541 taaatacatg attgatgacg taaaagatac ttgtgagcaa aaaggagtta agtacggtat
      601 tattccacaa agagtttatg gagcactgga tggtaatgga ttagtaaaat ttgctgaaga
      661 aattttgaaa gaaggaaact agtatggaaa atgtggaaaa agatgaacta aatttgttat
      721 caatgaacgt catactacat gcaggaaatg caagagatta tgtatttcag gcagttgaaa
      781 gagcttcaga tggagatttt actgaatcag ataaattaat gaaacaagct aatgatgagg
      841 tagtagaagc acatcgagca caaacaagca cactacaaaa agaagcagat ggagttgaaa
      901 ttccttactc accattattt ggacatgcac aagatacgtt aatgaccgtt aagacagaaa
      961 ttaatttgat gaaagaaatt atcaaaatat atagaaactt gaaggggtga caacgatgga
     1021 taaattaatg acatggatga ctgacaagtt tgcacccaag gtaaataaga ttgcgaagaa
     1081 tacatgggtg gcttccatcc aagaagcgat attaaccgcc atgccaatgg tttttattgg
     1141 atcatttgca acaatattat cgattgttaa aaattattgg tctagttttc ctgatttttc
     1201 tcttttaagt actttttcct ttggtctatt ttctatcttc ttagcctttc taattccaca
     1261 agcagtaatg aaaaataaaa aaattgcagg ggtagaaaaa caagcaggtc tagctggggt
     1321 agcattcttt ttattagtaa tatttcctag tatttctgca gatggtggga atatcacttt
     1381 tgatttgaat gcctttggta caggtgggat gatcgcagca ttagtatctg gaatgtttgt
     1441 gggcctcatt atgagcttgt tttctaaact aaaacttatt gatgaagatt cacctattcc
     1501 agactttgtt gcggtttggt ttaatacttt gatcccaata atcagtatat tacttgtggg
     1561 ttggctgttt acttttcaac taaaaatcaa tctttatgaa ttgatcaata gtctattttt
     1621 accgttgatt aatttaggtt ctggtttttg gggatttgta attatcagtt tcttagcata
     1681 tagttttcta tacacttttg gtatttcaag ttgggtgctt tatccagtac aagtagcaat
     1741 cactttacca gcgattgcac agaatcaagc aaatcatgca gctggaaaag ccgtaacaaa
     1801 tatctttact tctgaagttg gacaattatt tttaataggt gggggcggtg ccacactgtc
     1861 cttatgtatt atgttagcat ttttagcaaa atcaaaacgt ttaagaatga tagggaaagc
     1921 ttccctgatt ccatcaatat tcaatattaa tgaacctatt gtatttggtg ctcctatagc
     1981 attcaatccc attttgatgg tgccaatgtg gatcaatgga ttagtcggac cgatagttac
     2041 ttggtttgct atgaagtcag ggctggtccc aattccttat gaaccatttc aattatggta
     2101 tttgccggct cctattattg gctggattac tacaaaatct attatgggta ttttattagt
     2161 agttattcta tttatattat catggctcat ttattatccg tttttcaaag cgtatgataa
     2221 gcaagctgta gaacaagatc ttgaattgga aggagaataa tttcagatga agacaaatag
     2281 ttatccatat ttccccaaaa aattcctttg gggaggagcg attgcggcta atcaagctga
     2341 gggggctttt gatgttgatg gtaaaaaacc aaatacttca gatgttcaac cttatttaaa
     2401 aggactttcg aacgaagaaa ttcagagagt tgaaatgcag ggaatgacac ttaaacaggt
     2461 gaaccataat ttgaaggata ccactcaatt ttatcctaaa agatatggaa tcgattttta
     2521 tcatacattt gaatctgatt tagaattatt ggcagaaatg ggctttaaaa catttcgaac
     2581 atcaattgat tggtcacgaa tatttccaaa tggtgatgaa attgaaccaa atgaacgtgg
     2641 attagcttat tatgataagc taatcaataa aattatttcg ttaggtatgg aaccaatcat
     2701 tacaatgctc cactatgaaa caccaatcaa tgtcacttta agttttggtg gttggcaaaa
     2761 taaaaaagta gtcgatatgt ttgaacgata tgggaaagta ttattagata gatatgggaa
     2821 taaggtgaag tattggatcg tgattaatca gatcaacatg attcaggtag aacccttctt
     2881 atcattagcg atttgtagtg atcaattcga gaaaactgag gaagcagagt accaagcagt
     2941 gcacaatcaa atggttgcta gtgcaaaaat tagaaagtac gcagcagggt tgccctatga
     3001 tctacaaatt ggcacgatgg tagctgatgg aactgtgtat ccatactcag gtcatccaga
     3061 tgatgtagta ttagcaatgc gccataaccg gatgcaatat ttttttacgg atgtacagtt
     3121 taacggagaa tatcctcaat atgctaggaa ctattttaaa gagaatgaca tattcttgga
     3181 tattacgccc gaagaaatag aactattgcg tgagaataca atggattatt tagccatttc
     3241 gtattacttt tctcagatgg ttgatagcaa acagaatgtt gataaacctg gatctactac
     3301 gtccaatcct aatttggaac aaagtccatg gggatggaag attgatccac aaggacttta
     3361 caactcgtta tctcaatatt gggataggta tcataaatca atcatcattg ctgaaaacgg
     3421 atttggcatg tatgatgaac tggaagatgg taaagttcat gatgattatc gtatagacta
     3481 cttaagtcgt cacatagaac aagtaggtag agccatttat gatggtgcag atgtcatggc
     3541 ttattgtgct tggggaccaa ttgatattgt tagctgttct tcacagcaaa tgtccaaaag
     3601 atatggtttt atatacgtag atattgatga tgacggtaaa ggtagtggta aacgatttaa
     3661 gaaagacagc tttgattggt acaaagaagt aattgcatcg aatggcaaat atatttagaa
     3721 aaaatgaatg agaaaaacta gcatgtctat tgtgctagtt ttttctctat catatgaggg
     3781 gctaatttat atgcataata aagaacgttt aattgaatat ttaaagaaat tctctgatga
     3841 tagattcgtt cctgcaaaag aattagcaga agttcttgat gtaacagatc gaacaatcag
     3901 aaattatatt aagagtatta atacaaaaaa gccagattta attataacta gtagagatgg
     3961 atacaagtat gtggctaaaa ataattatgg gaaaaatcta gaattaacag atcagtcgat
     4021 cgatagtaga cgattttata ttttgcgacg tctatttaaa agttcagttc gaggcataga
     4081 tgtatttgat ttggcagatg ctttatatat ttcagatgcc agtgttcgat ctgatattgt
     4141 agctttaagt cgactttcag agaaatatga tttagttatt cgtcaaaaag aaggtcgcta
     4201 tattttagaa ggcaacgata aaaataaacg tcggttgatg attcaactaa ttcgctcaac
     4261 aaatcatgat ggatcaactt tcaatgtaga tattcaaaaa tttcttgata gtattccgtt
     4321 tggtgaagtt gtaaaaataa cgaaaacagc ctttaaaaac cataacttaa aaactaattc
     4381 ttactttttg aaaaattttg ttttacatct agctattgca atagatcgag caagagggat
     4441 atcacaatta atcgaggaaa gtgagccgca tcataataaa aaatatagat cttataacat
     4501 aatcaatgag attaggaaat ccatatttaa agaatttagt attaagctgt tactagaaga
     4561 tgttgaagaa ttaaatattt tatgcgaggg tgaatttagg aatacttcct ttgatttatt
     4621 aacttttgtt gagccaaagg ttcatgaatc cgtaaataaa gcattgggag aacttagtaa
     4681 gatttatttg attgattttt cggaaaagaa gtttaaaaat cgtctgttgc tacacattca
     4741 gagtttgtat aaacgttcac aggaaaaaaa ttattctaga aatcttagct tgatggaaat
     4801 aaaagtaaag tatcctgtgt tatttgatat ggcaatctat ctatcttcga tcttgtcaaa
     4861 tgatttaaag attactattt cagatgaaga aattgctttc ttagctttac atattggttc
     4921 atttgtagat agtcaaaaaa agtcagacaa gcaaattaaa acaacaatta taacgccttc
     4981 atataatact catgagatct atattaagga ccgaattgaa aaagaatttg aagatgagtt
     5041 aaaaattgaa gatgttgtag aggatatatt agatttatca aacgaacctg aattaattat
     5101 ttccacacaa agtatattag aaactgatag attaaaggta ataaatatac ctgtcattat
     5161 tatcaaagag tttcttacac ctcatgatgt aaaccaaatt aggagaaaga ttgagcaaat
     5221 taagtgttta agatataaaa aatttctaga aacttatttg ccaaaggcta ttccagaaga
     5281 attttatcta gaaataaagc ataaggtttc taaagaagaa gtgtttagaa ttattgaatc
     5341 tgtactcgtc aaaaaagaat atgttccaag taattttaga gatatgttga aagaacgtga
     5401 aaaaatctca ccaacagctt ttccaagtgg aattgcagtt cctcatagta tcaagtatga
     5461 tgcctataaa actggtctat acgtaatcaa ctctaaagaa actgtaaaat ggtctaattt
     5521 aaatgtgaac ctcattatag ccttatctgt agataaaaat gattcagagg agtttaataa
     5581 aattttccct agaatggtgg agcttcttgc cgaagaaata aatgtcagat atctgcataa
     5641 aagtttaaat cgtcaagaat ttatcacacg aatgattgat ctgatggtat ctgaagatta
     5701 ctatgaatga gaactacatt ttttaacttt ttcacagaaa atacagttaa aaagtaacga
     5761 agatattagg gaatgctgat aaaaaaataa gctggaaatg gaaggaaaac tagaatta
//
LOCUS       DAEGIQ010000137         5812 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000137 DAEGIQ010000000
VERSION     DAEGIQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.143"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..246)
                     /locus_tag="KMT45_002554"
     CDS             complement(<1..246)
                     /locus_tag="KMT45_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBE7005987.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEA"
     gene            complement(248..568)
                     /locus_tag="KMT45_002555"
     CDS             complement(248..568)
                     /locus_tag="KMT45_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7005988.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            867..1643
                     /locus_tag="KMT45_002556"
     CDS             867..1643
                     /locus_tag="KMT45_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBE7005989.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1658..1861
                     /locus_tag="KMT45_002557"
     CDS             1658..1861
                     /locus_tag="KMT45_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7005990.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1877..2197
                     /locus_tag="KMT45_002558"
     CDS             1877..2197
                     /locus_tag="KMT45_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBE7005991.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            2202..2372
                     /locus_tag="KMT45_002559"
     CDS             2202..2372
                     /locus_tag="KMT45_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005992.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPVGRD"
     gene            complement(2379..3161)
                     /locus_tag="KMT45_002560"
     CDS             complement(2379..3161)
                     /locus_tag="KMT45_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005993.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            3212..3919
                     /locus_tag="KMT45_002561"
                     /pseudo
     CDS             3212..3919
                     /locus_tag="KMT45_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
     gene            4097..4438
                     /locus_tag="KMT45_002562"
     CDS             4097..4438
                     /locus_tag="KMT45_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005994.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            4431..5102
                     /locus_tag="KMT45_002563"
     CDS             4431..5102
                     /locus_tag="KMT45_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBE7005995.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5108..5794
                     /locus_tag="KMT45_002564"
     CDS             5108..5794
                     /locus_tag="KMT45_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7005996.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
BASE COUNT         2135 a          916 c         1092 g         1669 t
ORIGIN      
        1 tgcttctgtt tcaaattttg agtgaaaagt tgtagaagta tcgtacaaaa ctgaaaattc
       61 aaaatgagag gcgctgaaat gtgcaagttc atgagataaa tgaaaggctt ctgcagtttc
      121 accgtataga ttttcattca aaaaaatgat tctgggtttt ggatagtaga aaccaggctc
      181 tttcatctcc atataaacta ctttcaaatt gtattcgctc aacatttctt tcaatttcaa
      241 atacatacta ttcattttcc tctaaagctt tagcaattgc aatcgcttta cgcattgtct
      301 ccttagatat ttcttttcca tcaaaagaaa aaacagtatc gtcttctgat aaatccacat
      361 gtttaggggt ttctctttct tctctaccta gaaggtagtc tacagagaca tcgaaatagt
      421 cagcaatccg acttaattca tctgaattag gagtattatt tttccacttt gctagataac
      481 cgttagaata tccaaaatta atttctaact gccgtataga tatctttttc ctcttcgcca
      541 agtcttttat tatttcatag gtattcatcg ataaaacaac ctttctaaat gtttacaaaa
      601 aaagtttaga aaaatacgca gaaataattt gactaattct gagtaatgag ctatactata
      661 tcttgtaaac agatttaaca actaaaaaga caacaaaaaa cactattgat atgtaaatgc
      721 agaccgccaa gaaagcttta aaatcaatgt ttttatgtct tatttaatta tgctcttagt
      781 atagagtatt actcagatac tgtcaattga atttagaaaa aagttgttaa atttgtttac
      841 gaatataaaa gaaaggaatg agaaatatga acacaccgca aatttttaat ttcgaacaaa
      901 acgaagttcg gacaatttta gtaaatgatg aaccatattt tgtaggcaaa gacgttgcga
      961 gtgttttggg ttattcaaac actaaagatg ctttgtcgcg gcatgtagat ttggaagata
     1021 agatggggtc gcgaatcacg acctcaggtc aatcaagaga gatgacaatc atcaacgaat
     1081 ctggtttgta cagtttaatc ttaaaatcaa aacttccctc tgccaaaaaa tttaaacgtt
     1141 gggtaacaag tgaagtgttg ccagcaatta gaaaacatgg aggttatcta actccagaaa
     1201 aagtagaaga agctttgctt aatccagata caatcattca attagcaact caactaaaag
     1261 aagaaagaac tggaagatta atcgcagaac aaaagattgc agagtacgaa cctaaaatct
     1321 cctatttaga tagcatatta tcttctacag attcagtaac aattagtcag attgcagcag
     1381 attatgggat gtctccacaa cagatgaata aattacttca taaactaggt attcagaaaa
     1441 aagtcggtaa ccaatggtta ttgtgcaaaa aacacatgaa ccaaggatac acaaaatctc
     1501 atacaactga gatcccgaaa gccgatggtg gcactaaaat tgtaatgaat accaaatgga
     1561 cacagaaagg gcgtctattt atctacgaat tactaaaaaa agaaggatat taccctcaaa
     1621 tggatttaga ggaaattggt tagaaaggag ttttagtatg actgacattg cagaaatcac
     1681 tcaacgagat agagaaaaaa tcaaagaata tgtcgaaagt tcaaagttct taacttacac
     1741 catgcttgct gaaagatttg gaattagcaa aagctactta tctttaattt taaacggtaa
     1801 aaagacttct gcagaagcaa acagaattat agattcgatt atcactatgt acgaattgta
     1861 aagggaggaa cagctaatgc aatatctaga agcaaaaatc ccaattccag aaggctatgt
     1921 aattatctcc caagtggatt atgaggagtt aaaaaaagct gatgatactg gtagatggat
     1981 gacgttgcca gaagtgctag aacggattaa cagaaaatat gattggttta cttctagagt
     2041 tttaaagaac ccaagatata gaaatattat cgatatcgag aaaaacaaaa atggatttgt
     2101 ctattatcca gttgaaggaa gagacacata tctattttta agaagtaaaa cacttgaatt
     2161 tttagaaaca aatttttcgg aaatcttaag gaggtaagcg gatgggtaaa tttaatagag
     2221 cactagtatt cagcgcacca ctaattatct acgctttagg tctttgggga agcagacaag
     2281 cattaatcgg cacaattgta tatatggtct ggatctttat ggggcttgat gaagctgaat
     2341 atagagcgaa aaagccagtc gggagggact gacttaacct aattaattaa aaattgtaac
     2401 aaataacgca aaaataaagc taatgaaatt gtattgttag gatctactcc aatagttata
     2461 tttacaattt cggttactac tagtcctatc aaagctgccc cagtttgctc taaataccac
     2521 attttatctt tgtagaaagc aggtgaagct tgttgttttt cagatggttc attagttata
     2581 tctttgttct tggctgtatt tatttcttca aaaagcttta tagaatcatc aaggactttc
     2641 ctttgctcat taaggatatc aaaaaactta gcctcaaact catcagatgg attagaagtg
     2701 tctttaaaaa gtagattttt tgtactaact tccggttcgg aagaaaatgc tttttctaat
     2761 tcttgggtag ctacccctat tctgaagtcg tacttagaca tgtcaacggc aaatttttct
     2821 gtgattttag agatatcaga aaaataaggt tttggcatag tagctgccat ttttttagca
     2881 atattaaaac ttggagattc taattgtttc tgtattgcga acaaagaagg aggaatcata
     2941 ttagcaatag atttgttacg tttctcgaca atttgaatga gagaatcatt gagtattgct
     3001 tgcgaagcta taaaagtatc ttgaattgca attaattttt tagcttgttc tatcgcaggc
     3061 tgcatagcgt taacgacatc tagagaagga gcaatggttt caccgattaa ttttgaagaa
     3121 gctatcaatt tttgataata aggagtatct gaaatatcca attatataca ccaccttttt
     3181 acctaaatta taccaaaagg gagagaaaaa aatgcaataa ttagtaattt tgaaaaataa
     3241 agaagctgtg actacgagct tacaagtcgc agaaacattc cgaaaggaac atagagcagt
     3301 tttaaaggcg attgacgatc taaaagatgg gcttgcacaa aagtatgcag acctattttg
     3361 ggaagatgtg tacatccatc cgcaaaacaa acaggcttat cgaattgtct atatgaatcg
     3421 tgatggcttt actcttgctg tattaggatt caacaatact caaaaagttt tagagttcaa
     3481 attgaaatac attgaagcgt tcaacgaaat ggaaaaggca attcgcaaaa atacagcttt
     3541 tgacacaatt gaagacatga tgatctatca attagaagaa atgaaagatg ttaaaaaaga
     3601 tgtttccatg cttaaaaata ctatgcgaat tagcggacaa caagagtttg aaattaagca
     3661 aaaaggaaat acgaaagtta tggaagttct agggggaaaa gaaagccgag cttatgaaga
     3721 aatcagcaaa aaagtattct caaaattttg gtctgaattt aaacgtacct tttcaatccc
     3781 aagatatggc gagttacctc gtaagagatt cgatgatgct gtttcattta ttgaaatgtg
     3841 gttaccagaa actgcgattc gcatggaaat cgatcaactg aacagacaac aaagactttt
     3901 tggtgatgaa aatgaataga gctgaagcgc taagaatcgg gacggtaatt gctaatcgct
     3961 ggtggagaca caataaacca atcatcctaa gccaacaaca tattgacaag caaaaagcat
     4021 ggcaacaaat aaaaaagtga ctccgccggc aagcaaagag tcacaaagaa aacacatcat
     4081 aaggagattt tagcatatgg aaaaagaact ttccactcta gatcaatatt tgactgatcc
     4141 tagttggggc aaatcgaata tcaaggaaac aaataatcga aaaatcagac gaaatctttt
     4201 gacggatgaa gaactagcat gtgatcaaga cgatttggga aattttgtga gtatttggga
     4261 tcatgtctat cttatccatt tatcgaggaa gtccaaaaaa cctgaatata tctatgtcat
     4321 cgaagatggc ttgattgatg cgctagaaga gtatgacaga gataacttga ttgatatctc
     4381 ttattacgga tcaggtaaga aatacattgc tgaaatggag gcagaatttg atgagtgaaa
     4441 acaaagggac aacgactttt gaaaaacttt ttagtcgcaa gttaaataaa atactcaaga
     4501 aaaaaggaaa ttttgattat ttatcttggg ctcacgcatg ggagattatg aaaaagaatg
     4561 atccacaggc aacggtaact attaatgagt ataaacacta cagagttgtt tctggaactc
     4621 atcaagactt tcttgttgag gaatataaac cttttcttat ggatgaaact gggacttatg
     4681 tatctgtctc agtaacggtt aaaggacaca cggaaaccga attatttcct gttttagatt
     4741 atcgaaacca accagttgtt aaaccaaatg caatgcaaat caataactca ttgaagcgat
     4801 gctttgtgaa agcattggct ctacacggac tgggattata tgtatttcaa ggggaagata
     4861 ttccaacacc acctagaatc gatacgaaga aattaaacat gctagagacg attctagaag
     4921 ctttcaatga gcagatgggt aaagatatga ccaaaacctt aatcgaatat gttaatgagc
     4981 agacagataa attagggctc ttagctgata acgttgaaac tattgaacag ttaagctatg
     5041 agcaatgtgc cttgatggag cgagcaatag cagctaagag aaaagaatta gataagaagt
     5101 gatatgagtg tttaaaccat taatcgattc atattcagcg gttctgaaaa agttcaaagg
     5161 aaaagacata ggtgcaacca tcaatgagga agtgaacatt gatcgactaa agacgatgta
     5221 tgacggctac gatggtgatc gagttattga aattcgtttt attgatccta gacgtttcac
     5281 agtacagcaa cgaaacttca tctatgcact cataggcgat attttcatcg atacaggcat
     5341 gccaacggac ttctggaagg aattctttta cttccgcttt gaaggtgtca cagggcgcaa
     5401 aataagcctc aaagatgaat cgaatacgac tgtgagtgat gctaacgtct tagcaaatat
     5461 catcttagat ttcatctttg aacatcatat tcctttcaaa gaaggctatg agattttacc
     5521 agcgaatcaa gaatattact tctacaaatg catcacaaaa agagtctgtt gcatctgtgg
     5581 caaaacagga gctgatatcg atcactttga caaagcgcta ggaagacgaa agcgcaaaga
     5641 agttgatcat tcagagtaca catttgcagc actctgcaga atccatcaca cggagaaaca
     5701 caagataggt gtgatcaatt tcaaaaataa atatcaaatc aaagggatca agttaaacca
     5761 ggaaacaatt aagaaactta gaataggagg ataaaaaata ttggctgaca ac
//
LOCUS       DAEGIQ010000138         5702 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000138 DAEGIQ010000000
VERSION     DAEGIQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.144"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            238..1629
                     /locus_tag="KMT45_002565"
     CDS             238..1629
                     /locus_tag="KMT45_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBE7005997.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1688..2491
                     /locus_tag="KMT45_002566"
     CDS             1688..2491
                     /locus_tag="KMT45_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBE7005998.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2524..3378
                     /locus_tag="KMT45_002567"
     CDS             2524..3378
                     /locus_tag="KMT45_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HBE7005999.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3375..4076
                     /locus_tag="KMT45_002568"
     CDS             3375..4076
                     /locus_tag="KMT45_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBE7006000.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4084..5151
                     /locus_tag="KMT45_002569"
     CDS             4084..5151
                     /locus_tag="KMT45_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBE7006001.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5144..>5702
                     /locus_tag="KMT45_002570"
     CDS             5144..>5702
                     /locus_tag="KMT45_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBE7006002.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIID"
BASE COUNT         2014 a          843 c         1108 g         1737 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga
     1561 ctagatgaag atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt
     1621 tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa
     1681 tgtaagtatg ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt
     1741 aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc
     1801 tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga
     1921 acatacgaaa aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg
     1981 attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa
     2041 tattgaggaa tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata
     2101 taacaatgca attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat
     2161 gtttgataaa aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga
     2221 ttattatctt tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac
     2281 attagtctat gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct
     2341 tggttctggt cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga
     2401 ttttataatt gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg
     2461 aaaattttat caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca
     2521 aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt
     2581 ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa
     2641 caagggttta ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa
     2701 aaattttatc aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct
     2761 tctaaaaatt ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt
     2821 attaaaactt atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg
     2881 ttagatgttg cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta
     2941 tatgctttat tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt
     3001 agtaagatac tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt
     3061 gctgaaaaac aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt
     3121 gtcggaccta aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg
     3181 gtgccattcg aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa
     3241 gtttttggtg actatatgca gcttccgcct gaaaaagatc aggtttctca tcatgaagca
     3301 gtttatattg atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa
     3361 ggagcagaaa aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg
     3421 gacaagatat cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt
     3481 tagaatcatt tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt
     3541 gggaagaaac gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca
     3601 ttacaggtgg atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact
     3661 attctgaaga tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac
     3721 tggtattgtc agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac
     3781 cttacaacga acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc
     3841 gtaacacatt gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag
     3901 catatgaccg ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca
     3961 tgatggtaga tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat
     4021 tgacaacaac agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga
     4081 aagatgatga cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat
     4141 catacaccaa aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga
     4201 atgatcggct cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa
     4261 atcaaagttt atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat
     4321 cttgaattag atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa
     4381 attcaagctg actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact
     4441 gatccaatcg gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca
     4501 gtagcaaccc aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat
     4561 cgaggcgata tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg
     4621 agagcaggct accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca
     4681 aaacacgacc tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt
     4741 ttatctgata cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt
     4801 gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct
     4861 gctattcttc atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa
     4921 aactttgatt tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa
     4981 gtaatttttg aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca
     5041 gtacttgaaa ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa
     5101 tcattggttc atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat
     5161 tattgttcct gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc
     5221 ccaaacattt actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg
     5281 gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc ataaagaaaa
     5341 cggcggatta agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg
     5401 ctttgttgac agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt
     5461 gaaaattcat gatgccgaca ttagctctgt tgaattgatt cctttttatg gtgatagata
     5521 taagaaagct aataaagaaa agaaagtaat tatattaaat aaaaaggaag caataaaatc
     5581 tgttttagaa ggaacgcaat tttatgcata cgcatggaat aaactttatc gtaaggaact
     5641 ttttaaagat aatcgttact tggatggaaa aacatttgag gatgcatata taataataga
     5701 tc
//
LOCUS       DAEGIQ010000139         5702 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000139 DAEGIQ010000000
VERSION     DAEGIQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.145"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            171..896
                     /locus_tag="KMT45_002571"
     CDS             171..896
                     /locus_tag="KMT45_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006003.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            886..1395
                     /locus_tag="KMT45_002572"
     CDS             886..1395
                     /locus_tag="KMT45_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006004.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1465..2913)
                     /locus_tag="KMT45_002573"
     CDS             complement(1465..2913)
                     /locus_tag="KMT45_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBE7006005.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2913..3629)
                     /locus_tag="KMT45_002574"
     CDS             complement(2913..3629)
                     /locus_tag="KMT45_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE7006006.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3610..3960)
                     /locus_tag="KMT45_002575"
     CDS             complement(3610..3960)
                     /locus_tag="KMT45_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBE7006007.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4104..4877
                     /locus_tag="KMT45_002576"
     CDS             4104..4877
                     /locus_tag="KMT45_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBE7006008.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5048..5414
                     /gene="ssrA"
                     /locus_tag="KMT45_002577"
     tmRNA           5048..5414
                     /gene="ssrA"
                     /locus_tag="KMT45_002577"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1657 a         1118 c          992 g         1935 t
ORIGIN      
        1 tcgaataaac agtgttcaaa agcttgcatc tacggtaata gaatagggca gcttcttcac
       61 aacctcttgc tttttacaaa atattttctg gttttaattc gtgatttcac taaaggaatt
      121 tcatacttta ttcatattca gtttctatac tttttataga ggtgaaaagt atgaaaataa
      181 tccgctatgc tactgctcga ttaatttatt acaaaaagca gacaatactc tattgtttat
      241 tttcaagctt ttctgctttt ttattgatag catgcctgac tctttaccat tcgcaatcgg
      301 ctttagccaa tcaagtccaa gatcggcttt catttattgg cgcttctgca tctacagagc
      361 ttcttccggc tttagaatta gctagccgat tttatataaa aggcatattc agtttatttt
      421 ttctatttgc cttccttttt atctttttct tttactattc agtcaggcaa aacaagcagg
      481 aattgatcaa ttggcgcctg actggtcttt ctaaaaccaa attattcctt tttattttct
      541 ggcagctgtt ctttcctctc tttctttgtt gtatcttggt tttattgtgg atcgtcagct
      601 tccagtcagt ttatgaagcg cttcttcaaa ggatcaattt tcttgtctta aagactactg
      661 aactgccaga tatccgtgat atcttgactt cggtaagcgg acttacaatt ccgatggatc
      721 aacagacttt ttttaccatc gatttccaaa atgatctttt tttcacagat atacttaagg
      781 gatttgccca aactcttatt gggctcagtg gatgctgtct cattcttagt ctattccctt
      841 tcactttttt tatccatcat ttgcaaagta ggaggcctgg acgtaatgaa acctgaatat
      901 ttgattcatg ctgaacactt gaccgttatt ttgtgtcctc tcgaaaaaca gcataaaata
      961 ttggagcact tgaaaaaaga ggttcttgtt gcaaaactgg ataattttgc gatcatccaa
     1021 aaaaattggc cgatgtttcc ttatctgaat cttaaagacc atgtcctgtt ggatgtaccg
     1081 gaaaaagaaa tcaaacaaga tcgtttggcc tatcaagaaa aacttgatat ctctccttcc
     1141 ttgcttaatt gtgcggcagc tgagttgacg tcttttgaaa aagtaaagct gcaattactt
     1201 catgccttgc tttccaagcg aaacaatatt gttattgaag atacatttga tgaacttagt
     1261 gtattggaga tccaagagct actccacttg ctttcttatt tagctcatga agaaaatcaa
     1321 ggtatcttac tctttactca tgatgctact attgctcaaa gtccttatat tgatcgttta
     1381 gagcccgcgg gctaaatcaa tcattttata gtaaaaacaa gcggtataga agatcgattc
     1441 atgatctttt ataccgcttg ttttttactc ttttttgatt tctacgtcag gcaaacgaac
     1501 agcgaatttt gttccttttc ctagttcgct ttctacttct aattttcctt tatgcaagcg
     1561 tacaagttgc tgcacgattg gtaaacctaa tcctgattca ccatattttg tattttttct
     1621 tgaaggatct actttataat aacgatccca gatacttttc atttgttgtt cagacattcc
     1681 aattcccgta tcttcaatcg tgatgatcgt ttcaagatag cctttttcta aagtaatcgt
     1741 gatttggccg ttttcagtaa actgtatcgc gttttgaata atattgacca tgatttggac
     1801 aaatcggtca taatcagcgt aaacatcaat atcatcaacg gctttcaatg tcaatgtatc
     1861 tcctgctgct tctgctttag cttctaactg tgcaacaata tttttcaatg tttctgttcc
     1921 attgaatttt ttaatgacca tactgatttg attcgtacgg attttttcat aatccaagtt
     1981 ttcattcacc aaacggatca gacgctctgt ttcatttttc atcaatttaa tcgcgttttc
     2041 tcgctgattt tcagggattg cattgtattc taacccttcc agaagtccgt tgattgtcgt
     2101 aagaggtgtt ctcatctcgt gagaagcatc tgccataaat tgtcttcgac gattttcttg
     2161 ttcttcaatt tctgcttgag attctttcaa tgaattcgtc attttattga aatcatctgc
     2221 taactcatca aattcatctt tgtcatgtac ggggagctgt acatcaaaat taccattcgt
     2281 cacttctttc gtcgcattcc ttaaccgatt gatacgctta acttggaatg ctgcaaaagc
     2341 atagctggca ataactgcaa cgatactgga aaaaataaat cctttgaaca ggttcagtgt
     2401 aattggacga acgctgttat cgatatttcg cgcaggttga ctaactacaa gtgctccata
     2461 gaattcatga ttcaaattaa atggcacaag tgcgtaagag gtcgcctgtg cttgtcctaa
     2521 aatattttta ttcgatgtga atttttcttg acgtccattc ctcagatttt tccattgatc
     2581 agctgttaag gtaaaattca gtaaagctgt attagtcgga taaatgacac gttcgttttt
     2641 atcgataaat ataaagttca cattttgttc tgtcagtact tgctctgtca gcagcaatgc
     2701 attttgaaac gcctgatctt gactaaactg cgggagcgta tcggcatagg tttgcgttgt
     2761 cttttcaact gattcagcat atccaaacag ttgctggtaa ttgttttctt caatcgtttg
     2821 ttttgtcagt tgtgtaaaag aaactccaac gatcaataag atcaacgcga ccattccgca
     2881 aaaagctaag agttgctgat acaaataacg cattaggcaa caccagaatc atcgaatttg
     2941 tagccgactc cccaaacagt ttggatgact tgtggtccga ccttttcgat tttttgtcgt
     3001 aatttcttga tatgggcgtc cactgtccgt tcatcaccaa agtactgata atcccagact
     3061 aattcaagca actgctcccg agagaatact tgacgtggtt tttttgctaa agtaaacaat
     3121 agatcgaact ctttaggcgt cagtccttca attggccggt tgtccagata cgcttctcgt
     3181 gttttcgtat tcatcttgaa atgactagtc tcgatatcaa aatgatcatc tgaaactgct
     3241 gcttcttctc ttgcttctgc caactcacta cggcgatgca aagctttcat tcgagcaata
     3301 agcgtcagag gactgaaagg ttttgtgaca taatcatctg cccccatctc taagccgatc
     3361 acctgatcgc tttcagaatc tctagctgtc agcatgataa tcggcacagt acttgaaact
     3421 ttgcggatct ctcttcctac tagcataccg tccagtgtgg gcaaattcag gtctagtgta
     3481 atcatatccc aatgttgtgg gttttctaaa aaggcatcga gcccttcttt tccatcatac
     3541 ttgaaagttg cttcccaacc ttcatttaag aaaaacatct gcatcatttc agaaacagat
     3601 tgattatctt caatcattaa aatattcatt atcgttccct ccagtttcca ggtcatattt
     3661 ctgtaatttc ttttctgtca taataaaaat aggcataatt ggaaatacta gcagaagcat
     3721 ttcaatcaga aaataattcg tatgcccaga tgtctttaaa taatagccga tcaatgccat
     3781 cagtaagccc ataagaaaaa accagtttcc gcttgtcttt tgagccagcc gccaatgagc
     3841 gtctgttttt gcggcaagag gcgaatgata cccataaaaa ggcagatttc cttttgaggg
     3901 cagaatccga aaaagactgc caattattaa gaataagata ccaatactga aaaaaatcat
     3961 tctttccctc ctaaattctc ttcttatagt atactctaaa ttgttgactg aaccaatatg
     4021 ttcaggaatt cttcacgaaa ggtttgtctt ttgttcattc tttgttaaga taaacttatt
     4081 ccaataaaaa aggagtttta tttgtgtcaa attatcgtgc aatcactttc tttgatcttg
     4141 atggaacatt actagatgcg aaatcaaaaa tcacacccga agtggcaaaa gctatgaacg
     4201 cattaaaaga aaacaatgtt ctccccgtca tcgctacagg acgtacagaa gcagaaatcc
     4261 actctatcat ggaagatgcg ggcattactt ctgcggtcac catgaatggt tctttcattc
     4321 gagtagacgg cgaagaaatc tattctcaaa cattttcttc agaagaatgt caaaaaatgt
     4381 atgatcatgt acagttgaac ggtcatgaac tttcttacta taacgatcag aaaatctggt
     4441 gtaccggtca tagccaaaca atgatcaatg cttacgactt cattcattca gatgttccgg
     4501 aaattgatcc tttaggcttt aaagaaaaca cagtaaatat gttgctgatc ttatctcaat
     4561 caggtgacga atactatcac gaacattttc ctgagctaac tttttatcgg aatggtcctt
     4621 actcgatcga tacagtgaag aaaggaatct caaaaggggc aggtgtcaaa cgtctgcaaa
     4681 aagaacttca gctggaaaaa gttcctactt ttggatttgg cgatggtccg aatgattttg
     4741 ccttgttgga agcttgcgac aacaaaatcg ctatggacaa cgcctatgac ggattaaaag
     4801 aactttctac ctttatcaca aagaaaaata cagaaggcgg aattgtccac gcattgaagt
     4861 attttgatct gatttaagat ctttttttga agctacctct tttcttaaga aaatcgctta
     4921 ttttttctta agaaaggggc agttattata taaataatgg gatatgattc gcttttttat
     4981 tgaatttatc ccaatatatg ctatactaat tttgtatgaa aaatacacgt aacccttgtt
     5041 agctaacggg gacgttacgg attcgacagg cacagtcgag cttgaattgc gtttcgtagg
     5101 ttacgtctac gtaaaaacgt tacagttaaa tataactgct aaaaacgaaa acaactctta
     5161 cgctttagct gcctaaaaac agttagcgta gatcctctcg gcatcgccca tgtgctcgag
     5221 taagggtcct aactttagtg ggatacgttt caactttccg tctgtaagtt gaaaaagaga
     5281 acatcagact agcgatacag aatgcctgtc actcggcaag ctgtaaagtg aatccttaaa
     5341 tgagttgact atgaacgtag atttttaagt ggcgatgtgt ttggacgcgg gttcgactcc
     5401 cgccgtctcc atatatgtat ggttgagaca gataaacatg tatgaaaaac gttgttaaat
     5461 caacgttttt ttattttggt caaggagaga aaagtagaga aatacaaaat tttttgcatt
     5521 gattttgcat tcaaaaaaac attcaaacat ttagaaatcc ccaatacgta ttcaggaaaa
     5581 tctattttaa ggtttacaaa aagaactttt gttcgtacac tctttttgag gtgaactttt
     5641 atgctgatgg aaggaaaaac acaattatgg tttaagcatg ttccttcaaa taagtttttt
     5701 ta
//
LOCUS       DAEGIQ010000140         5689 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000140 DAEGIQ010000000
VERSION     DAEGIQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.146"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(488..1297)
                     /locus_tag="KMT45_002578"
     CDS             complement(488..1297)
                     /locus_tag="KMT45_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE7006009.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="KMT45_002579"
     CDS             complement(1297..2043)
                     /locus_tag="KMT45_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7006010.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="KMT45_002580"
     CDS             complement(2061..2528)
                     /locus_tag="KMT45_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7006011.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="KMT45_002581"
     CDS             complement(2528..2938)
                     /locus_tag="KMT45_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBE7006012.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="KMT45_002582"
     CDS             complement(2949..3962)
                     /locus_tag="KMT45_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBE7006013.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="KMT45_002583"
     CDS             4179..4913
                     /locus_tag="KMT45_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7006014.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="KMT45_002584"
     CDS             4897..5607
                     /locus_tag="KMT45_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7006015.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAEGIQ010000141         5675 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000141 DAEGIQ010000000
VERSION     DAEGIQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.147"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            186..1178
                     /locus_tag="KMT45_002585"
     CDS             186..1178
                     /locus_tag="KMT45_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7006016.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1238..2659
                     /locus_tag="KMT45_002586"
     CDS             1238..2659
                     /locus_tag="KMT45_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBE7006017.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2652..4151
                     /locus_tag="KMT45_002587"
     CDS             2652..4151
                     /locus_tag="KMT45_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBE7006018.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4190..5584
                     /gene="uxaC"
                     /locus_tag="KMT45_002588"
     CDS             4190..5584
                     /gene="uxaC"
                     /locus_tag="KMT45_002588"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBE7006019.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1865 a         1051 c         1164 g         1595 t
ORIGIN      
        1 aaaattgata ctcatctaat taaatgagca ttccccgctt tcaagaatag cataattaaa
       61 aataaaaatc caagtaattt ttaacgtgtg caatcttttc atatcaaaga tctattgact
      121 tatcgtagtt atcgtaatat gatgaatgcg ttaacaaaaa gtgaaaaagt aaggaaaatg
      181 atggaatggt aacgatcaaa gatatcgcta aagcagctgg cgtttctcat acgacagttt
      241 ctcgtgcttt gaataacagc agtttaataa aaaatgaaac aaaacaaaaa atacgacaac
      301 tagctaagga aatgaattat tcgccaaact atagtgcaaa aggtttagtg aatcaaaaaa
      361 aatatctaat cggactattt ttctcaagtt tgcataaagg aacttcatca aatttcctag
      421 cagattccat tgcaggcatc cattcagtat tagacacaaa ttacagttta tctgttgaaa
      481 gtatcgatgt tgttgattta acagaaatca acctgcaacg ctacgatgga ctgatcgtta
      541 tgagtcaaag tgatgatgat cagcctttca tcgactatct caaagaaaaa gaatttcctt
      601 tcgttgttgt caatcgtcac atcaatgatc cagcggttat caacgtcact gccgatgatg
      661 cggccggtgt tatgcaggca gtagattacg gtattggatg cggacatacg aagatcgctt
      721 atattggcgg aaagaaaaat tttcgttcga caaaagaacg ggaaaaaggg gtattgaaaa
      781 gtctaaaaaa agcgagcatc cctgtcaatc tagcctattt tttaacagga gattacagct
      841 tagaaagcgg cctccaaaac atggaatacc tattatcact ttctgacctg cctactctgg
      901 ttttttgcgg caacgacgac atagcgattg gtgcattgag agctgtacat ttagctggcc
      961 tccgtgttcc ccagaacatt tctctgatcg ggttcgatga ttctccagtc gtctcctatc
     1021 tcaatccgcc gcttaccacc gtacgtaagc ctttgaaaga aatgtgtcaa gaaggaacaa
     1081 agcttttact agatttgatc gaaggcagga cgatcgaaca atctaaaatt gagctctcca
     1141 ccttgttgat ccttcgtgag tcgatcaatt acttgtaaac tacaagtaat tttatataaa
     1201 aatgttaacg tgtgcataac acagaaagga agtttatatg aaaaaactga caaaagaatt
     1261 agtgaaaaaa aacgaagcac ctgtaaaagt tcttcagttt ggtgaaggca attttatgcg
     1321 tggatttatt gattggcaga ttcagcagct gaataaccaa caattgttta aaggtaatgt
     1381 tgcaatcatt caacctttag aacaaggtct tggaaatatg atgaaccagc aagataattt
     1441 atatacggtc attttacaag gactcagcaa tggcgaaatc gttgatactt ccgagattat
     1501 caccgtagta gaacaaatga tcaatcctta tgatgaatgg gatgcttatc tatctctagc
     1561 agaaaatgac gacttggcct atatcgtctc caatacaaca gaggcaggaa tccaattcgt
     1621 ggagactgat aatttaacag atactccccc acagagcttc ccgggcaaac tgacagcctt
     1681 tttataccga cgtttcaaat taaacaaagc tggctttacc atcattcctt gcgaactgat
     1741 tgacagaaac ggcgaacgtc taaaagaaat cattttacgc tacgcagaaa aatgggattt
     1801 agaacctgct tttgttgagt ggttagaaaa agaaaatgtt ttctgttgta gtttagttga
     1861 tcgcatcgtc ccaggttatc ctcgcgaaga tgcaggtgtt ttcaatcaac ggcataacta
     1921 tgaagatcaa ttgatggtca aagcagaacc attcatgctt tgggtgatcg aagggcctaa
     1981 agaattggag aatcaactac cattcaaaca ggctggcttg aatgtcatca tcacagatga
     2041 tatgactcct tatcgtgaac gtaaggtgca tttgcttaat ggaccgcata cagcgatggt
     2101 ccctcttgct ttgttggcgg aattggaaac tgtagaagaa gtaatgaaag atcctttatt
     2161 ctccgcatat gttgatcagt tattcaatct agaattgatt cctatgcttt ctttacctaa
     2221 agatgaatta gccatttatg ctgaccaaat caaagaacgt ttcttgaatc cttttgccca
     2281 tcacaaatta gaagcgattt cattaaatag tgtttcaaag ttcagcaccc gtctgcttcc
     2341 agtgttcaag aagtatatcg aagaacaaaa tcaggtacca ccgttaatca ctgtttcact
     2401 agctgcgcta cttcttatgt atcgaggcga tcaagtaaag ccacatgatg atgaaaagac
     2461 gataagcgaa tttactgatg cttggtctga taatggaaca gctattcctc gattattgca
     2521 aaacgctgcg ctttggggag aagatttgtc tcaaataccg aatgtgacag atacagttca
     2581 agaaatagct gcaaagatcg atcaaaaagg tgtccggtca gtattacaag aaatgaaagg
     2641 aacgatggtc catgcttgaa accctgcaac caacaatgaa attaaatcga cgagacagtg
     2701 tggtcgtcgc attaacccct attccaaaac aaacacaact gacgattgat cagcaaacga
     2761 tcaccactct tgaagatatc ccgcaaggtc ataagattgc attgggtgat ttgaaagctg
     2821 gagacaacgt gatcaaatat ggctatccaa tcggtcatgt gacgacagac gtaactgccg
     2881 gtcaatggct gcacactcat aatgtaaaaa ccaatctttc tggtgaattg gattatcgat
     2941 atgaaaaaga tgtccatcca agtcattacc tttttgagaa ccgaacattt caaggttact
     3001 tgcgaaaaaa tggaaaagta gggattcgta atgatttgtt tttagtacct accgttgggt
     3061 gtgtgaatgg cattgctgaa ctgatcgtca aacagttcaa ggaaaaacat ccagatctgg
     3121 gacagtttga ccatattacg attttaaagc atccttatgg ctgttcgcag cttggaaaag
     3181 atcatcaaaa tactcgagaa attttagcgg acgcagtaaa tcatcccaat gccggaggag
     3241 ttttagtatt tggattaggt tgtgagaaca atacagttcc tgaattcaaa aaaattttag
     3301 gtgaatatga tgaagaacga gtgaaatttc tagtggctca agatgtttac gatgagatcg
     3361 aacaaggtgt ggctctatta gaagagcttt taacagcggc ggaaaatgac cagcgcatat
     3421 cagtcccgct ctctaagttg aatgtcggtt tgaaatgcgg cggttctgat ggtctatcgg
     3481 gaataactgc taatccttta cttggtgcat tttctgacta cttgattgct caaggaggca
     3541 gcacgatctt aacagaagtg ccagaaatgt ttggcgcaga acaagtattg atggcacgag
     3601 cggaaaatga agaagttttt gactcaatcg ttgacttgat caatgacttc aaacagtatt
     3661 tcttgtctta cggggaacca gtttacgaaa acccatctcc tggtaataaa gcaggtggga
     3721 tcactacatt agaagataag tctttaggct gcacgcaaaa atcaggaagc tctcctgtcg
     3781 ttgatgtgct acaatatggc gaaaaaatca gaaaaccagg gcttagttta ttgcaagctc
     3841 ccgggaatga tcttgtagct gcctctgctc ttgcttcatc cgattgccag ttggtcttgt
     3901 ttaccacagg aagagggact ccgtttggct catatgtccc aacggtcaag gtatctacga
     3961 atacaactct ttttgaacgc aaaggtcatt ggatggattt taatgcaggc gtcttattag
     4021 aacaaccaat ggaagtaatc ttagaatcgt ttgttcaaaa aatcatcgct gtagctagtg
     4081 gagaagaaac gaaaaatgaa caaaatgacg ttcgagaaat tgccattttt aaaaacggag
     4141 taacactttg attttcataa aacagtataa aaagaaagaa ggaagattta tgtttttatc
     4201 tgatgatttt ttattaaaag acgattgggc gaaaaagctt taccattcct acgcaaaaaa
     4261 catgcccatt attgattatc actgtcactt agatccaaaa gaaatttacg aaaacaacaa
     4321 tttcagcaat ctgacagaag cttggttagg aggcgatcat tacaaatggc gcttgatgcg
     4381 agcttgcggc gtgcctgaag aaaaaatcac aggaaatgct tctgattttg aaaaattttt
     4441 agcttggtgt caaaccgtac caaaaattat cggaaatccg ctttacagtt ggtcacatct
     4501 tgaattgaag cgtttcttcg gtattgactt gttgataaat gaagaaaatg cagagaaaat
     4561 ttggacacaa gcaaatgaaa ttttggcagc tcctgatttt cgccgtcggg aaatcgcaaa
     4621 aaaatcgaat gtagaagtga tttgtacaac agatgatccc gcagacgact tgcactatca
     4681 tcagttgctt gctcatgaag aaccagattt gaaggttcta ccttcattcc gtccagataa
     4741 agcactcaat attgaaaaag aaggatttaa tgcatgggtc acactactgg aagaagcagc
     4801 tgaccaaacg atcaccacct atcaagaact gatagaagtc ttagggcagc ggatagaata
     4861 cttccatcaa catggatgtc gtgtttccga ccacgcttta gacatcttac gctataaaga
     4921 agcggatgaa acagtattag aagagatttt tcaaaaagca cgagctaaca aactattatc
     4981 ttcagatgaa atcgatgctt atcttacaga aacactgata catttgaccc acttctatca
     5041 ttctcataat tggacgatgc agctgcacat ccatgcttat cgtaactgta atacacaaat
     5101 gttggaaaaa ttagggcccg ataccggcta tgatggaatg aatgatcttt ctttgactat
     5161 tcctctgcaa aaattattga accgtgcaga agaaacggat cagttgccaa agacgatcct
     5221 ttattcattg aatccgaatg actatcctgc gattttagct ttgatgggct gtttccaaaa
     5281 agaacagaat ggaaaactgc agttaggttc tggatggtgg tacaacgata ctcgagctgg
     5341 tatgcgcgat caattgacac aatttgctga tggtagtgct ttggggaatt ttgtcggcat
     5401 gctgacagat tcaagaagtt tcctgtccta cactagacat gaatatttcc gtcgtgttct
     5461 ttgtgaattt ttaggcgaaa tggtcatgcg aggagaagca cctgaagatg aacaactttt
     5521 aggaagtcta gttcaagcga ttagttatac aaatgctaaa aattactttg gcttttttgc
     5581 ctaattgatt aatagtaaca gtaacgaatc aaaaacagga tagtgaaata acggaatccg
     5641 tttatccacg ttttagagaa gaagacggac caaat
//
LOCUS       DAEGIQ010000142         5616 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000142 DAEGIQ010000000
VERSION     DAEGIQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.148"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            80..781
                     /locus_tag="KMT45_002589"
     CDS             80..781
                     /locus_tag="KMT45_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBE7006020.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            778..1899
                     /locus_tag="KMT45_002590"
     CDS             778..1899
                     /locus_tag="KMT45_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBE7006021.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1914..3146
                     /gene="pepT"
                     /locus_tag="KMT45_002591"
     CDS             1914..3146
                     /gene="pepT"
                     /locus_tag="KMT45_002591"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBE7006022.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3278..4186
                     /locus_tag="KMT45_002592"
     CDS             3278..4186
                     /locus_tag="KMT45_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBE7006023.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4221..4493
                     /locus_tag="KMT45_002593"
     CDS             4221..4493
                     /locus_tag="KMT45_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBE7006024.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4504..5550
                     /locus_tag="KMT45_002594"
     CDS             4504..5550
                     /locus_tag="KMT45_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBE7006025.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1826 a          972 c         1222 g         1596 t
ORIGIN      
        1 ggagatatgg gaattttttt attcctagtc tccttatttt atttggaaaa tgctataatt
       61 atctagaagg agtgaagcta tgaaccattt agatttatct cagcgcttag ccgcagtggg
      121 tgactttgtc cctgaatccg ctcgtttagc agacattgga tcagatcacg cttacttacc
      181 cgttgcattg atattaagag gaaaaatcga atatgcagtt gctggtgaag tcgttcaagg
      241 tccttatgaa tcagcacgta agcaagtcaa aaaagatgga ttagaagacc gtatcaccgt
      301 acgccttgca aatggtctag atgctatcca gccacaagat ggtattacag ctgtcacgat
      361 tgcgggtatg ggaggttcac tgatccgaga tatcctagaa gctggcaagt tgaatcaacg
      421 cttaagcgga caagaacgtt tgatccttca gccaaacatc ggagagcgta cattacgatc
      481 ttggctgcaa gaaaatcagt ataaaatcca atcagaaaaa atacttgaag aaaataaaaa
      541 aatatatgaa atcattgtag ccgaaaaaga acaaaaacca gttacttaca atgaaatgga
      601 gctgatgttc gggccatttc tgcttgaaga gaaaagtgag atcttcaaat caaaatggac
      661 aagagagctt caacaaagaa aagctatccg tgaacagctc aaacaagcag ctaatcaaga
      721 ggcacgtcaa aaacaaatcg agcaggaaat ccaaatgatt gaggaggttt taaagccatg
      781 acaatcagcg gacgcacttt catccaaaag tttgaaagct attgtcctca gtggctagct
      841 gaagaaggag acccagtcgg actgcatatc ggaacattgg ataagccaat ccaacgagta
      901 atgatgacat tagacgttcg tccagaagtg gtcgaagaag caatcaagaa aaaaatcgat
      961 ttgctgattg ctaaacatcc tccaatcttt cgtcctgtaa aacggctggt tactgatcag
     1021 ccacaagaaa aaatgtatgc agatttgttg aaacatgata ttgccgtcta tgcagcacac
     1081 acgaacatgg atattatttg ggatggactg aatgactggt tttgtgaact tctcggtatt
     1141 gaagtggaga gttatttagt aaagactcat gagattcatt ataaaaaact ggccgtttat
     1201 gtacctgtgg atcatgcaca gaaaatgcgt gaagtattag ctgcagctgg tgctggtaca
     1261 caaggtgatt ataccggtac aagttttacc tcgattggac acggtcgatt tactccggaa
     1321 gctggtgcac aaccagcgat tgggaaagta gggaagacag aacaagttca agaggcaaaa
     1381 gttgaggtta ttttgccgga aactatcgaa aaacaagtga ttcaagccat gcggtcagct
     1441 catccttatg aggaacctgc ttatgatctt ttcgctatag atgaaccagt cgaaatgttt
     1501 gggttaggac gagtaggtga attgccgcaa gaaatttcta tagaggcatt tgtagaacaa
     1561 gtaaaagaag cgttccaact agatggactg agaatagtcc agccaaaaaa tgcaaaatcc
     1621 tctgtgaagc gcattgcgat ttgcggtgga agcggtgaaa aattttatcc gcaagcaatc
     1681 gcccaaagag cagatgtcta tatcacggga gatatctatt atcatacggc tcatgatatg
     1741 caaagcgctg ggttgattgc aattgatccc ggtcattata ttgaatcgtt atgcaagcaa
     1801 cgatttatcg agaaatttga atcttggaaa caagaagaaa attgggatat taactttttc
     1861 gtttctgaaa caaatacaaa cccatttcaa tttaattaaa aggagagaat actatgtacg
     1921 aaaatctttt gccacgtttt ttacgatatg tcaaaacaga aacacgctcc gatgcaacaa
     1981 gcaaaacgac tccatcgact cagacacaag ttgcttttgc gcaagaattg aaaaaagaat
     2041 tagaagagtt aggcttagct gatgttttct ataatgaaga taacggattc gttatgggaa
     2101 cattaccaag caatgttgat catgaagtgc gttccattgg atttatttcc cacatggata
     2161 cagcagattt taatgcagta ggagtcaatc cacaaatcat tgaaaactat gatggcgagt
     2221 ctacgatcgt actagatgcg gaagaacgct atacattgaa tacaaaagat tttccaaatt
     2281 tgaaaaatta tgaaggccac acattgatca caacagatgg cagtacatta ctgggagcag
     2341 atgacaaatc agggattgcg gaaatcatga ctgcaatgga gatcttgatc aataatcctt
     2401 ctatccctca cggtaccatc aaagtggctt ttggtcctga tgaagaaatc ggtgtcggag
     2461 cggataaatt cgatgttgag caattcgatg tagattttgc ttatacagtc gatggtggtc
     2521 ctttaggtga gttgcagtat gaaacgttta gtgctgctca agcaaatatt acgatccaag
     2581 ggaaaaatgt acatccaggg acggcaaaag atacaatgat caatgctcta cagttagcaa
     2641 tcgattttca taatcagctt ccagccgatg aagttccaga aaaaacggaa ggatacgaag
     2701 gtttcttcca tttgatggca ttggatggga atcctgaaga agcttctatg agttatatca
     2761 ttcgcgatca tgatcgtgaa aaattcgaag cacgcaaagc acaaatcaca catatccaag
     2821 aagagttgaa tcagcgattt gatcaagaac gaatcaaagt agatatgtat gatcaatatt
     2881 acaacatgaa agaaatcatt gaaaaagata tgagcatcgt ggaattagcg aaacaagcaa
     2941 tgatcgagct tggtatctct ccagtgatcg aaccagttcg tggaggaaca gatggttcaa
     3001 aaatctctta catgggtatt ccgacgccaa atctatttgc cggaggagaa aatatgcacg
     3061 gacgattcga atttgtttct ttacaagtaa tggaaaaagc aacagacgtg attgtaaaaa
     3121 ttgcagaatt gaacacaaag agttaaaatc gaaaaagaac agcttatgac aaggcgttcg
     3181 tcccaatgat ggaatgtcat ctgtctaagg ctaaagggca ggacaatgga attggttgtc
     3241 ccgccctctt ttttgaagag taaataggag gtaaagaatg gaaaagctgc tagtttttta
     3301 taacaaaagt tcagggaaag atgagggtga gaaattagca cgctggtttc aagattatgt
     3361 aaaagaaaag cagccagaat tatatgtaga actagctgaa actggcccat cgattgatga
     3421 tgcggaatta gtcaagaaag cagaagccat agaagctgat actttagtaa ttataggtgg
     3481 agacggcacg attcatcata tcgtccaagc gttccaaaaa acattatctc aataccatgt
     3541 cggattactt ccaggtggaa ctgtcaacaa ccttgcacgt gttcttgaga ttcccctcaa
     3601 aaaagaagaa gctgctgacg tgatcctgga aggcaatgta cggaaaatag actatggtca
     3661 agtcaatgat gaagtcatta tctctacact gacaatcgga atcttagcag atacagcagt
     3721 ttggatcagt caagaagaaa aacagcgtta cggctcgtgg atctttatca agaagttttt
     3781 taagttattg ctgaagaaga aaaaatatca tttggattta gaaacagacg aggagcattg
     3841 gaaaggaaaa tcccaactgt tgacagtcac gatgtctaac tccgtgggcg gttttacaaa
     3901 ttttgacgac tcagcgaccc tagatgatgg aaaatttcat gtaacgatcg taccttcctt
     3961 agatattttc cgatttattt tttatttgcc tagaatcatg agaggcaaat tctatacgat
     4021 ccccggcatt acttatttca cagccaaacg catgaaaatc caagcgcagg aaaaatctgt
     4081 gggcactcga acggatggag acacgacaga tgatcttcca gttgaattat ctgttgtatc
     4141 aaaaggatta acagtttatt caccaaagaa agaagaaaaa gcttagaaat agtagttaac
     4201 agaaagttgg aagaagagta atggatgtat tgttacacaa tcctttgtgg ttttaccttt
     4261 tcattgtgaa tttttactta ttttgtctga tggggtatga taaacatcag gctagaacta
     4321 gcggctggcg tgttcctgag aataatttga tatttgtcag cctgttaggc ggtggtttgg
     4381 gaggattgat ctctcgaaaa gttttccatc ataaaactcg gaaaaagaag tttactatag
     4441 gattcgctgc aggtgtactt gttgatttga ttctcattta ttttttccat taaggagcag
     4501 gagatgtcgt ctaaaggaac gaaaactaaa atcattctga atctgctttt gctgattgtg
     4561 atttttgcca ttatttatta tttaatcaat caatcatttg cagatatctt tgccgaattg
     4621 ctgtctacca gtgttgttgt tttggcagcg gctgttatat tagggacagt ttaccaaatc
     4681 gctgaagggc gctcaatcaa agaaattgct gaacctttcg cgccgaattt tacaacgaaa
     4741 gatgggttct tgacctctag ctatattgcg ttttaccgaa tcgtttcgtt tggtacagga
     4801 acgctgatct cggagattta tttttataac aaaaaaggaa tgaagctatc ccagggagca
     4861 ggggtgacag cactccatat gattatgtac aaactggcag tgatcactta tgcattagct
     4921 ggcttggtca tccaattctc tcttttttat tctaatggtc catcaatgat ctggtttatc
     4981 atcgctggta tccttttgac gatcgtgatt gttgcctttt tattggcgat gtccgtcagc
     5041 ttgaatgttc aaatattttt tgtcagctta tcgaataagt tatttaaaag tgagcgtttg
     5101 cggaaatggg tggatacatg caatcagcag atctactctt taagggaaac ggtccaaacc
     5161 attatccaag acaggactgc gctattgcgg atttatctat ggaatatgtt aaaacttgta
     5221 ttttggtatg tacttcctta tttgattctg attgaaaacc atcctaaaat agattttcta
     5281 ctgaccattt cgtttatcag ctttgcagta gtgctttcag gggttattcc tacgcctgct
     5341 ggtattggtt cctttgaatt tgtctatttg ttattattca gaccagttgt tggaacagtt
     5401 gacgcagttt cttcgctttt actgtatcgt ttcagttcct ttatcttgcc atttctctat
     5461 ggatttgtgt atgtgattgc tgaacggcga agagtgatca aagaagaaat aatagaagta
     5521 aaaaaagaga ataaagaaaa tcaagggtag gtcttttata agatctaccc tttttatcaa
     5581 atcaaagaaa aacgttatgg ataagataat tttacg
//
LOCUS       DAEGIQ010000143         5576 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000143 DAEGIQ010000000
VERSION     DAEGIQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.149"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            86..904
                     /gene="purR"
                     /locus_tag="KMT45_002595"
     CDS             86..904
                     /gene="purR"
                     /locus_tag="KMT45_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBE7006026.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1407..2780
                     /gene="glmU"
                     /locus_tag="KMT45_002596"
     CDS             1407..2780
                     /gene="glmU"
                     /locus_tag="KMT45_002596"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBE7006027.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2874..3845
                     /locus_tag="KMT45_002597"
     CDS             2874..3845
                     /locus_tag="KMT45_002597"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBE7006028.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3851..3953
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4019..4588
                     /gene="thiT"
                     /locus_tag="KMT45_002598"
     CDS             4019..4588
                     /gene="thiT"
                     /locus_tag="KMT45_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBE7006029.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4669..5442)
                     /locus_tag="KMT45_002599"
     CDS             complement(4669..5442)
                     /locus_tag="KMT45_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006030.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1829 a          984 c         1166 g         1597 t
ORIGIN      
        1 ttattttttc ataaaatgct cgaatttttg aagaaaaact tatataatgt aaaacgataa
       61 aatttcttaa aaggagttgg aataggtgaa agtacgtcgc agtgaacgtc tagttgacat
      121 gacacagtat ttattgaatc acccgcatga attgatttcc ttaacaagct ttgccgaacg
      181 ttatgaatct gctaaatctt ctatcagtga agatttggga atcatcaaga aaacatttaa
      241 agaacgaggg tatggaacct tggaaacaat cccgggtgct gcaggtggtg tattgttcat
      301 ccctgaaatt tcatatgaag aggcgaaaaa atatattgaa tctctagcag aacgtctatc
      361 agaacaagac cgtctgcttc caggaggata tgtttatctc tctgatttgt taggagaacc
      421 tgaactgctg cgtcaagttg gaagaatcat tgcttctaaa taccttggtg aaaaaatcga
      481 tgctgtaatg actgtagcca caaaaggcgt acctattgcc caagctgtgt cctattatct
      541 gaatgcgcca ttcgttattg tacgccggga ctcaaaaata acagaaggat caacagtaag
      601 tgtcaactat gtgtctggat cttctgaaag aattgaaaag atggaactgt ctaagagaag
      661 tttgaaacgt ggttcacgtg tccttgtagt ggatgatttc atgaaaggcg gcggaacagt
      721 caacggaatg aagagcttga ttgaagaatt cgaatcagaa cttgttggca tcactgtatt
      781 tgccgaatca aaattcaacg gtcatcgcgc catcgatgat tatacgtcac tgctttatgt
      841 caaagatgta gatactcaaa cgaaaaatat tacggttgtg ccaggaaatt atttttcaga
      901 ataagaggtt gtgaaaaagc cgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg
      961 ctttccacat tttttcataa atttggctta ataccgagaa actggctttt gaacaccgtt
     1021 tatctagagg ttgtgaaatc agcgtcctgt cctgagtatt agctcaataa acggaaaaat
     1081 tgctcctcat attttgccgt ttattgagct aataccgaag gtcagctgat tgaaaaacgt
     1141 ttaatgaaga ggttgtaagc ctaccgttta actctaagtc acaagaaacg atttcaaaaa
     1201 acagcttttc atgttttatg agtagtgtga cttgtgaccg aggagttggc aggagagcac
     1261 cggttaccaa atagataggc ccgttatggt ctatctattt ttttatccag aaaacgctgg
     1321 gataaaagaa aatcacttgt tttgttgaaa aaatgggtat actgggataa actgaaatca
     1381 gcaaactata tttaggagtg acgcagttgg acgcacgtta tgcaattatt ttggcggcag
     1441 ggaaaggtac ccgcatgaag tcaaaattat ataaagtttt acatcccgtt tctggtaaac
     1501 ctatggtaga acatatcatt aatcgggtca gtgaaacgaa accagatgaa gtgattacga
     1561 tcgttggaca cggagcagaa caagttaaag cacaactagg agaacgcagt aaatatgcgt
     1621 tacaagcaga acaattggga acaggacatg ctgttttgca agctgcttct ttcttggaag
     1681 gaaaaaaagg aacaacgtta gttatcagtg gcgatacacc attattgact actgaaacgc
     1741 tgaacaatct ttttgaatat caccaaggaa aaaatgcaag tgcaacgatc ttgacggcac
     1801 aagcagaaaa cccaacagga tatggacgaa tcatccgtga ccatattggg atcgtagaaa
     1861 aaatcgtgga acaaaaagat gcaacaccag aagaagctct tgtacaagaa atcaatacag
     1921 gaacttactg ttttgacaac gaagctttgt tcgatgcgtt aagcaaagtt ggaacaaaca
     1981 atgctcaagg tgaatactat ctaacagata tcatcgaaat cttaaaagaa gaaggccata
     2041 cagttgctgc ttatcaaacg gatgattttg aagaatcaat gggagtcaat gatcggatcg
     2101 cgttagctaa agcaaatgag atcatgagaa agcgaattaa ccagatgcat atggtcaatg
     2161 gcgtaagctt tgtcgattca gcaacgacat acatcgatgc aggcgtagaa atcggtccag
     2221 acacattgat cgaagcaggt gtacaaatcc aaggaaatac agtcattggt tctgattgtg
     2281 tcatcgggtc acattcaaaa atcgtagaca gtaggattga ggatcacgtc gtcattgaaa
     2341 actcagtcat tgaaagcagc catgtcaaga aacatgcaga tgtgggacct tatgcacatt
     2401 tacgaccaaa agctgaaatt ggcgaaaacg tccatatcgg aaactttgtg gaagtgaaaa
     2461 atgctcaaat cggcaaagga actaaagtcg gtcacttgac ttatgttggt gatgctacgc
     2521 tgggagaaga aatcaatgtc ggatgtggtg ttgtcttcgt caactatgat ggaaagaaca
     2581 aacatcatac gactgtcggc gaccacagct ttattggttc tagcacgaat atcatcggac
     2641 ctgtcgaggt agctaaaaac agttcgattg ctgccgggtc aacaattaca gacaatattc
     2701 cagaatatgc gctggcaatt gcgagagcaa gacaagtcaa taaagaagga tatgcaaaaa
     2761 aactccctta tctaaactaa gacttttgca aaatcacttg atttatttaa aaaatatgac
     2821 tactatttat taggtagaat tctagagttg tgaaaaagaa agtggaggtc cccatgtcaa
     2881 aacattactt tgacccaaga ttaaaaattt ttgcgttaaa ttccaatcgc ccattagcag
     2941 agaaaattgc agcagcagtc ggcgtcgagt taggaaaatc atctgttact caatttagtg
     3001 atggagaaat tcaagtaaac attgaagaaa gtattcgtgg atcgcatgtc tatgttattc
     3061 aatcaacgag cagcccagtc aatgataact tgatggagtt gctgatcatg attgacgcac
     3121 tgaaacgagc aagtgcaaaa acaatcaacg tagtcatgcc ttattatggc tatgcgcgtc
     3181 aagaccgtaa agcacgtgca cgggaaccaa tcacagcgaa attagtagca aacatgattc
     3241 aaaaagctgg tgcaacaaga atgctgactt tggaccttca tgcagtccaa atccaaggtt
     3301 tctttgatat tccagtcgat catttgatgg gtgcgccact tattgccaat tatttcttag
     3361 aaaaaggaat caaaggcgat gatgttgtcg ttgtttcacc agaccatggt ggtgtcacac
     3421 gggcacgtaa attagcggaa tttttgaaat ctccgattgc gatcatagac aaacgccgtc
     3481 caaaagcaaa tgttgcagaa gtaatgaata tcattggaca agtcaaaggc aaaacttgtg
     3541 tactgatcga tgatatgatc gatacagcag gtacgatcac attggctgcc aatgctttga
     3601 aagaagctgg tgcaactagt gtgtatgctt catgtacaca tccagtatta tccggccctg
     3661 ctttacaaag aatagaagat tcagctatcg agcgtctagt tgttacagac tcgatctatc
     3721 ttccagaaga tcgtaaaatc gacaaaatcg atgaagtaag tgttggcgga ctaatgggag
     3781 atgcaatcaa acgtatccat gaaaacaaac cagttagtcc attgtttgaa acaaaaaata
     3841 aataataaat aaactcacaa aggggagtcc aattgggctg agattgaatc tatttctaaa
     3901 cccttcgtac ctgtatcggt tatgcgagcg taggaattgt gaataaacag ctgtttgact
     3961 attttcgaaa gctgcttcct cctttgtgac ttttttattc aaaggaggaa gtttttttat
     4021 gggaaaaaat agagtttggg tagaaggaac gattgttgca gcgttagcga tggtgttgtc
     4081 gttgattccg atccagatcg gcagcagttt ttctatttca ttagggcaga ttccattgac
     4141 tctttttgcg ttaagaagag ggacaaaggc agggatgctg gcaggtttca tttggggcat
     4201 cctacatttt ccgttgggac aagtttatta tttaagtgtc gttcaagtgt tgattgagta
     4261 cccgattgcc tatacttttg caggcgcagc agggctcatg gcttcaaatg tccaaaaagg
     4321 ctatgtagac gataatacga aacaaattag aaaaagcatc gtgtttggtg cattgattgg
     4381 cgcactttcc agatattttt ggcattttat tgcaggagtg gttttctggg gagcgtatgc
     4441 actatggggg atgaatcctt ggctcttttc tttcgtaatg aatggtgcca gcgggttgag
     4501 tacagctatt gtaacgatca tcgttttatt agggatccaa caaacgactc cttcgatttt
     4561 tgtgccagat aaacgacagg agctgtaaat aaaaaagaag caagaaactt gagacagaat
     4621 catgtcctag tttcttgttt cctttttatt ttttgataag aaatagatct acttcaatgg
     4681 cagataagaa agatcggcat cttctaccaa ttgccagtgt cgatcggtga cagagtagat
     4741 taaagtagcg atgatttcct cttctcgata ccgggtgcct tttaatttcc aaatataatc
     4801 ttcaatcaca tagattggga tacgatattt accatcgatc aaaccaactt gatcaaaaga
     4861 aaaaatgatc ccttcctgca tcaaattacg catttctttc aataattcca cactgatttt
     4921 ttcaacaggt gtttcttttt tgaaaaaaga tttttttaca ttgatcttat ctgacaagat
     4981 cgaacttaat ttactagcaa aaatcctata aaattcatct aaatagcgat aataaatccg
     5041 attcagctga tcattaagat cgaagtatac aaaattattc tgtagtttat aaaaaaacgg
     5101 cgaatgaaga tgtgttttca tatgaccaaa atataataat tctgaaattt ctaaaggtgt
     5161 caattctttc agcatcgtta aatcagtaaa gtcgatccac ttcaattcat cgaatccacg
     5221 cctgttacga gaagaggaaa agaaacgctg aatattttct gtaccacgga ttactttcag
     5281 ccctgtatgg gattcatatt ctccagcaga agcagatgga tcaagcaaga gcaagttttg
     5341 aggaaaatgc acgatactgt ttgcaaagtc agtagcagtc agcccttttg ttaaaacagc
     5401 attacttgtg gtatcaatat gtacataaac tgactcagtc atcatgttcc tcctcttatt
     5461 tcctcttaat ctcttttatt ttacaacatt tgcattaaaa aagcttgttt cttttatttt
     5521 aaaattccat tacaaaatca cgttttcatt gaaaacactc tcattttcaa ttaatt
//
LOCUS       DAEGIQ010000144         5446 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000144 DAEGIQ010000000
VERSION     DAEGIQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.150"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(22..252)
                     /locus_tag="KMT45_002600"
     CDS             complement(22..252)
                     /locus_tag="KMT45_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006031.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            806..1048
                     /locus_tag="KMT45_002601"
     CDS             806..1048
                     /locus_tag="KMT45_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBE7006032.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            1399..1653
                     /locus_tag="KMT45_002602"
     CDS             1399..1653
                     /locus_tag="KMT45_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HBE7006033.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            1682..2941
                     /locus_tag="KMT45_002603"
     CDS             1682..2941
                     /locus_tag="KMT45_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBE7006034.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            2994..3890
                     /locus_tag="KMT45_002604"
     CDS             2994..3890
                     /locus_tag="KMT45_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE7006035.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            3901..4770
                     /locus_tag="KMT45_002605"
     CDS             3901..4770
                     /locus_tag="KMT45_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBE7006036.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            4780..5446
                     /locus_tag="KMT45_002606"
                     /pseudo
     CDS             4780..5446
                     /locus_tag="KMT45_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         1728 a          828 c         1103 g         1787 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa gggggcaaaa atgcgtttac ctttgatata tctcagattg
      361 aacatagtgt ttattttatg tcggtattgt tgatttatag ctgttttgag ggtgttagat
      421 acataataga atatagtggt tttgtttacg tgcattacac ggaaaagctg ctcgtatcaa
      481 agaaatccgt cgttaatcat tgttatcaga ctgagaaaga acccttggta ttcaagggtt
      541 cttttttctt agcatgtaat ttgaacaccc tgatccagtt tttttcttta attattggta
      601 ggggagaata atggattaga ataaatctac ctataagtag tagtaaaaaa aagagcacta
      661 tgataatatc tttcttaaga gtaaccgctt taataaaaaa tccagaatat tctaacataa
      721 atggagtatt cttagaatca aaaggagaaa gaagatggtt tatacttata cggagcaaga
      781 aaaaatgaaa gtttgagaca cattcatgaa tgatggaaaa gtaaaacaag taccaagttc
      841 aacaaaaaag aaaaacatcc tattgaaaga agtcttgaaa cgctttgacc atggagtgac
      901 ttatacagaa acagaggtta attcgatact gctgaacgta ttttctagtg gcgattatgt
      961 cgaacaaaga cgttatctaa tcacatttgg ctttttcaaa agaagttcag acggtcgggc
     1021 atatcagatg atgggaattg aaaattagaa gttttaattt atactcattg aaaaaagttc
     1081 aagtatgcga tattggttct acaccgcata cttgaacttt ttttgtcatc tgtatctata
     1141 atacaaagcc aagaacgtta ttcaaaatat ggatagctat gataaaagct aatgagcgat
     1201 tcgcagttaa ataagcaatc gtcagtaaaa tcgttccagg agcgtacatg aaaaaatcaa
     1261 ttggatgcgt tacatgaata aagataattc tcttttattt gtacgaataa ataccaaaac
     1321 gtacctccta aaattagatt caaagaagat agcaaagagg aaaaagatag ttctgcagca
     1381 gctatagcgg catgtggatt gctagaactt tccaaacaac tgcctgtttc agatcctcgt
     1441 catttcgatt acgaagagat agcaatcaaa atcttggaag aattacagaa aaactataca
     1501 acgaaacgag ttaacggatc aaacggtctg cttctccatg ccgtttatga taaaaacagc
     1561 ttaaaaggtg tagatgaatg tgtgatctgg ggcgattact tttatgtaga aggaatcact
     1621 cgtctagcta aaacatggta ctgttactgg tgatgtataa gagaagagga ggattcatat
     1681 aatgaagcga tggaaaaaaa tgatgattgg atcaatagtt ttaggaatgt cactcgtatt
     1741 tggtgcttgt ggtaatcaag aagacagtag tgaagaatca gcaaatgaaa ttaccgtttc
     1801 gacatggaat tatgaaacaa cacctgaatt caaagcgctc tttgaagcat tcgaaaagaa
     1861 aacgggaatc aaagtgaaag cagtagatat tgcttcagat gattatgata cgaaactcac
     1921 cacgatgctt tcttctggag acacgacaga tgtattgacg atgaaaaatc ttttgtcgta
     1981 ttcaaattat gctttacgag atcagttagt cgatcaaaca gaacgaatta gagaattaga
     2041 cactgatgct gctgaagaaa catatgaaat gtatgatatc gatggaaaaa catatgcatt
     2101 gccatatcga acggactttt gggtactata ttacaataag aaaatgtttg atgatgcagg
     2161 aatcgaatat ccaaaaaatc taacttggga cgaatatgaa gatttagcaa aaaaacttag
     2221 taaaaatgac ggacaagttt acggagcata tcaacatatc tggcgttcga caatccaagc
     2281 tattgcagct gctcaaaacg aggcgaatct tgttgaacca gattatcaat atatgactga
     2341 ttattatgat cgagcattga ggatgcaaaa agaaggcgcg cagatggatt ttggcacggc
     2401 taaatcaacg aaagtaacct atcagtctca atttgaagaa caaaaagcag caatgatgta
     2461 catgggaact tggtatatgg ctggcatact cgcaaacaaa gaagcaagta agacagaagt
     2521 agaatgggga attacagcta ttcctcagaa gaaaaaagga gaatcggtca cgacgtttgg
     2581 ctcaccaaca gcatttgctg tcaataaaaa ctcaaagaaa caaaaagcgg cgcaagaatt
     2641 tattgaattt gcagccagtg aagaaggagc aaaagtatta gcaggagtag gagtagttcc
     2701 ttcatatcgt acggatgaga ttgatcagct ttattttaat ctcgctggaa tgccaacgga
     2761 tgaaatttct aaaaaagctt tttctcctga tgaaatcaaa cttgaatttc cgattgatac
     2821 gtatgggcct gcaatcgata agattttaca agaagaatat gacttagttt tggttggaga
     2881 tgaaacgccg gaaaaaggaa cggtaaatat ggagaaacgt gtagctgcgg aaaaagaata
     2941 atcatgaaag gagagaagcc aagagataaa acactcttgg tttcgtcatc gaaatggaga
     3001 atagtataaa taagaaagct acaaatcatt tgcgtagaaa acgaacgtgg atcgcttatt
     3061 cgtttatcgc cccgaatttc attggttttt tcgtttttac attaatacca atcctgtttt
     3121 cgcttgtact agcatttatg aaatgggatt ctttttcaac gccagaattt gctggactag
     3181 ataactttcg taaaatggcc aacgatgaga cattttggat ttctttgaaa aatacttttt
     3241 tatataccgc tgctgtagtg cctggcacac ttgtttgttc gttagggcta gctttactac
     3301 taaatcagaa aattagaggg atgaagcttt ttcgtacgat atttttcttt ccgtatgtta
     3361 cttccttagt agcaattgct gttgtttgga atatgttgtt tcatccaaca gtgggaccta
     3421 ttaatatctt tttaagacga tttattgaac atcctccagg ttggacttca agttcaaatt
     3481 gggcgctttg ggcaattgta attgtcagta tttggcgagg gatgggttat tacatgattc
     3541 tataccttgc aggactacaa agtatccaaa aagaactata tgaagcgtct tcgctagatg
     3601 gagcaaaccg ttggaaacaa tttttgaatg tgacattacc ttcgttgaga ccaacaacct
     3661 tctttgtaac gatcatgctt gtcatcaatt gtttcaaggt gtttgacctt gtgcaagtga
     3721 tgacggatgg cggccctggt cgagcgacaa atgtgctggt ttaccaaatc tacaatgaag
     3781 cattcaaaaa atttgatttt ggttatgctt cagctatttc acttgttttg ttcgtaattg
     3841 ttttgggcgt tacggttgtt caattttctt ggaataagcg tcgtacttag gaggtgtgat
     3901 atggaagaga gtgttcgaaa tgaaagaaaa aaagcaaaaa ttcgttcaaa aaacatgccc
     3961 tttattcaat ggttgggtat gattctgctg attgtattag ctttattgac gatcattcct
     4021 tttttatgga tggtttctgc ttcattcaaa gcaaataatg aagtgttaac gattccgatt
     4081 caatggatcc ctaaaacttg gcatttagag aattatctgg ttatttggtc tcgtattcct
     4141 ttagtgacat tttttaagaa tacagctttt ttaagcgttg tgatcacgtt gattcagttg
     4201 tttacttcta gttttgctgt ttacgggttt tctgaactaa attttaaagg gcgcgatatt
     4261 ttatttttag catatatcgg cacgattgct gtcccttggc agtcttatat gatcccgcag
     4321 ttcattatga tgagaaaagt gggtttaaca gatacgttgt ggtcgttggt gctattacaa
     4381 gcgttcaatg cttttggtgt gttcttgttg aagcaatatt acagtagtat cccagattct
     4441 ttatgtgaga gtgcgcggat cgatggctta tcagagtggg aaatctatcg caagatcatt
     4501 ttacctctta caaaaccggc gattgccagt ttgacgatca tcacattcgt taatacatgg
     4561 aacgattata tggggccatt catttatttg tcttctacgg aaaataaaac aatccagtta
     4621 gggttgaaaa tgtttattgg gctatttgat gcagagtacg cattaatcat ggctgcctca
     4681 gttgtttctg ttcttcctgt tgcttttata tttttagcga tgcaaaaata ttttgtagaa
     4741 gggatcgctt catcaggaat gaaggaatag gaggtgatta tgaaagagca gcgttttgac
     4801 aaaaatgttt atatggaatt cttttattgg ttgtatcaat cattggcaag tagcctttgt
     4861 ttttggtgtg tcaatatctt gtatatagca gctgcgttcc ttttgtcgat agatggaagg
     4921 aatgtctttt cctttatttt ttctttactt tttattggtc caggttgcat cgtgatgata
     4981 ggtatgacag aagaattagc aaaagaaaaa agtttttcac cagtacgttt gttcataaag
     5041 acgtttcgcc aattttggct aaagggattt ttttattggc tatttgcttg gatagttagt
     5101 gtgataatga tttttgattg tttctttttt atacgttttt cttatggaaa atggttgatt
     5161 ccgttgtttg ttttacttgc ttgttttact tgcttgttta tcagtttcct ttagcatcaa
     5221 ctgctggtat tttcaggtga gaaatccagc gtcgaaacca aatcaagttc tacggatagc
     5281 gttctactat acgttgaaaa aatggtatgt gagtttacta gattttttac tattgacaag
     5341 tttgtttctc ttctttttcg taaagcccca atggtgtatt ctactaggtc cttctatcgt
     5401 atttggtttg atttacttca acaatcgtaa attgatgaga actatg
//
LOCUS       DAEGIQ010000145         5427 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000145 DAEGIQ010000000
VERSION     DAEGIQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.151"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            101..685
                     /locus_tag="KMT45_002607"
     CDS             101..685
                     /locus_tag="KMT45_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006037.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            696..941
                     /locus_tag="KMT45_002608"
     CDS             696..941
                     /locus_tag="KMT45_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006038.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            947..1669
                     /locus_tag="KMT45_002609"
     CDS             947..1669
                     /locus_tag="KMT45_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006039.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            1800..2543
                     /locus_tag="KMT45_002610"
     CDS             1800..2543
                     /locus_tag="KMT45_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006040.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2549..2914
                     /locus_tag="KMT45_002611"
     CDS             2549..2914
                     /locus_tag="KMT45_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006041.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            2930..3460
                     /locus_tag="KMT45_002612"
     CDS             2930..3460
                     /locus_tag="KMT45_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006042.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3622..3930
                     /locus_tag="KMT45_002613"
     CDS             3622..3930
                     /locus_tag="KMT45_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006043.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            3926..4345
                     /locus_tag="KMT45_002614"
                     /pseudo
     CDS             3926..4345
                     /locus_tag="KMT45_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4482..5282
                     /locus_tag="KMT45_002615"
     CDS             4482..5282
                     /locus_tag="KMT45_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006044.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
BASE COUNT         2137 a          683 c         1038 g         1569 t
ORIGIN      
        1 gtatgacaag tttaccataa aaaaaaatta aaaagcgaga catatttatg ttgttgtgtt
       61 atcataataa cataaatatg tagagtgaga tgatgaaaaa atggatttga aacaatcgtt
      121 ttacatctgg ttatatcctg ataatcctat gtcaataatg gctgatattt ttgtagttag
      181 catcttattg tatttatttt atctaatttt ctttcacaat ttaatcgggt acatgattct
      241 tgattggaaa ggaatgttcg atatagaaag aaaaccattt gctaacaatc aaacctatac
      301 cgtcttcgaa agtggagatt gggaagaaaa tgaagttgat gtgatccatg aaattggatt
      361 agctttatca ggaatacaag atgctgatgt tttggtatca atgacaacgg atactggata
      421 caacgcttat gaggtatttt atcttatgta ttttatatgg cgtgatggtc aagaaattat
      481 caatggtcgt gctgaaaaaa caatcaattc taatactgaa atttcttttt gtatgggtga
      541 aggggaaatg attgtatata aagatgggtc agcaaacatt aaaacagcct ttggtgatat
      601 tgaaacaagt attaaggaac ttagggaata tgtgcagaac ccatataatt ttcaatattt
      661 agcaggaaga ataaatttag tttaggagta aaaatatgaa aaaaattata aatcaaccaa
      721 caaatcactt gataattgaa ggtgttattt tcgtaacaat gtattttact gtaggaaatg
      781 gcatctcaaa tacaatcatt caaaaaaatc cacaatacct attatttagt ctttatgctt
      841 ggattgtaca aatggtttgg gtttccatat tgatgctgtt gttttattgg atagtacggg
      901 ttattctttt attgttaaac aaaagaaaaa aggataggtg aataaaatga aatcaactaa
      961 attgaaggtt gtattgttat cgacagcagc attaataacc cttacagcat gtggtacaac
     1021 taatagtgct aataataaca aagacacaaa gtctagtagt acgatggtta ctaaaaacag
     1081 cactacatcg gcatcaacaa caatgtcttc aatgactgaa aataatgctg aaaagtatca
     1141 aaaatacgaa gacattaata aaggtcaaaa gccggatgaa gtttataaca actttattaa
     1201 tacagtaaaa aatgatgaaa attatgggaa tgatccattg caaaataaaa atctgactat
     1261 cgttctttac aaacctgatt gtccagattg tcaaaaagca gaatcaaaaa tcatgcaaga
     1321 aataaaagaa ggatttagtt atgctccaac attaatgcat gatattacca taaataaacc
     1381 agcttacgag tattacgcag ttgctgtaga tattactgat ggtgtacctg agtgggtaca
     1441 aaacattcct ggtctggctg atgagaacaa tcaatatcat acgccaacag ttgcagtagt
     1501 aacaccaaag aaagttaacg gcacactata ctgggatgta atgggaatga caacaaatac
     1561 aactgatgaa atgattgaaa gtacaactaa atattatatg gatttgggag catataaatt
     1621 atatcaacaa tcagagcctg ttatggacga tcaatccaaa gttaaataga tctttagggc
     1681 gtaagcccta tttttttaaa attaagtgat aacaaataaa tgatttctga aacaaacgtt
     1741 cttactcaac tactcgaaac acacaagcta attaactaga ttataaagga gaatgcgtaa
     1801 tgaaagaaat cagtgcaaat gaagctcata ttccgcaaaa gaatcaggtt ggtatatata
     1861 aggtgcttca tggtgaagga atttataaaa tacagttact taccggttac aataatggtg
     1921 caaatgggaa gtggttaaag gcaacaagta ttcgtgaact tagaagtgaa gtagaaaaaa
     1981 ttaggcttgg tctcacaagt gaagaaatag ctaaactgaa gggaaataat tcttttgtaa
     2041 ttgaaaaaga aaaggaaaaa catctacttt atcgtgagct gaaaattgaa gaatactatg
     2101 aaattgattt acctgaaaaa ttaagaatta ttgatcaacg ttcagaagaa aaatggaaaa
     2161 ctgaaatata tccaagctta aatgactaca agacatcagg aaagcccatg tataactaca
     2221 caggcttttt aacctacaca caatacaaat atgcctttcg caaattaaaa ggcattaaac
     2281 aagctgaaat tgccctgtat gagatgaaaa aagtggatag attgaaattg attcaagatt
     2341 tagtgcaaaa tacatggaat caagcgattg tcgagctaga aaaagaaaat gggaaaattt
     2401 taactaataa atatctgatc gtaaatgaaa ttgaagaatt aattggttta ttttcatgtg
     2461 aaaaaggtga agtcttcttg aagtcatcac caacctattc aattttggac agtccgtatc
     2521 agtttataca ggcaaggaga tgagttagat gtctagcttg aaagatgact atatccaagc
     2581 aaaacaagag tatgaaaaaa tacagatgtt gaaatctgag aaagtcgaat tatttttaac
     2641 acaaaaatta gcagaggatc atattgctaa cttagatgct aaaactttag aggaatatga
     2701 tcggcagttt aatcagatac atcacaatat cattttgctt gttgagcagg caaagcgaag
     2761 gtttgaaaaa gcggaatatg atctattaga tcaaggaatt gtatgcttgg ataagggatt
     2821 gcgaagatat tatggtgcag agttaaaaaa gcaaaatgta gatagaaatg cactaaaaca
     2881 tgaaatctta gaattgataa tgcaacgaat ttgaagggat agaaagcaaa tgtcacaaaa
     2941 cattagtgct gtaaaacaat taatagaaga agatttagag accacagttc agagattagc
     3001 ggaacgaatc cattttgcaa attatcaaac catctctaac tggtcaagca ggaacagaaa
     3061 tccggaagaa attccaatga aattttttta tcgaatcgga aaagaatatg gtgttgatcc
     3121 gataaaaatt ttcgagaaga ccatgattta ttactatcat ggaacaccta acgaagtgtt
     3181 agaacatctt ttggaagagg caaaagaaaa atcatcaata ttagagctat cgcaaaatga
     3241 caaagttatt caagtgaaat gcgttgatcc aacaggaaag attattgtcg gttttgatat
     3301 tgaaagaaaa tccaaaatag attttattac acaattaaaa aagataaata atttagatat
     3361 tctaacgctt agaccagatc atgaacagtt gtttatgagt tttaaaaaac aattgaaaga
     3421 aatacaaaaa agatataaca aggtgtttcc taatgcgtga aaagctcaaa gaaatcagag
     3481 ttttttggat aattaacaaa gaatgtatca tcttatatac tcttggattt cttagcatga
     3541 tcttgttttg gtggttatat ttaaaacagt aattggagag ggtcaagagg atttttatca
     3601 agataaggat gtggaaactg catgtggaat aatagattaa aaacaggatt gttattaata
     3661 gttatttcct gcgctatgat gattggtatg aggatacaaa gggaacaatc ctattttgaa
     3721 gtttctgcta acaacgtaat agaaaaatgc tattacggtc aacattattg gagtgaggaa
     3781 gtcagagaaa atatagatcg tgaatatgtt caaagaattg tttgggatgc atattctatc
     3841 aaagattatc ctaaaagttt aacgagtaga ttattttaca gtgaaaaaga taatcaaaaa
     3901 ctctcagatt tgatgatgaa aaaagtatga ctattattac gaaagagaaa cagagtgcta
     3961 tattcgaggg gaaaaagcct acaacaaaag tgactattgg ggtgacaagg cgaagcgatt
     4021 aactgcaaat tttagagtgt atgaatcaga gtttataaac ttgacctttc ttaatagtgt
     4081 ttttattgat tatgtaatta ctacgaaacg cattggaagt tattcaagta cctacgcatc
     4141 cttagttcca atgttaaact cgatgagccg tttcttaaaa gaaagagaaa agaaagagct
     4201 acagtatatc aaagaaatca tgccggattt tgaaatgcac gaatggcaaa ttgctttatc
     4261 agattggaaa ttggcgaata aggttcgtag aataacacct tttcaagcca agcgcttttt
     4321 gagagctatg aaatttgtgc aataaaaaaa gccaccagac aagttgatgg agactaacac
     4381 aattaccaac agttgtaatt gtgttagctt acaaattaat tatggtgttt agtcaatccc
     4441 aaattgtgaa atggcaccaa taattggaaa ggaaaaatga aatgtctgaa gatttacgag
     4501 agctgaagtt acgaaagaag cagtatgatc agtatttttc cggtttgttg aataaacata
     4561 ttgaagaagg acttgatcat gattttggaa aagatgaata ttgtttatat tgcgaacgtt
     4621 acaatgaaaa aatgtctgaa tatctacagc tgaaaaaaga gatacagaaa ttagagaaaa
     4681 aagtacatgc ttggaagtat aaagattcca tgtctggtta tgttgaatct caaaataagc
     4741 caagaaaaaa gaagcaagcg tatgttctaa agatggtaaa tggggaagag ttaattgtat
     4801 tagcgtacag tgaagaagag gttcgagaac tcgttgaaac acctgtcagg aaaaaggttg
     4861 aaacaataaa tgagatagac gacagccaat acaatagcat ctatattgaa acaagtcatg
     4921 gtggatataa gaaattagca actttcctta gaagttctaa agttgggata atttctaaaa
     4981 aagacataag tgctgttacc gatagttttt gtactttatc tgttcctaaa gcttatttcc
     5041 aatacgatag taaaagattt gaacgcttag agaaattctg tagaccgtat actgtattga
     5101 aaactaaaaa atttaataaa actaaaagag tttctataca gttaccgact ggtcaaatct
     5161 cattaggtga aaaagactat ctgataaaag taggaaaaga gttaattata aaagtaccgg
     5221 aacacttatt taaagaaaat tttgagatac gtgaaacctt ggtatggaaa gaagaaaaat
     5281 agtttggaag acatagaatg gtataagggg aaattgaaat ggcagtaaat aaaatattgc
     5341 aggtactaat catagacgaa tttggcggag gagaatggtt tgatgtgcag tccatagact
     5401 ttgaagaaga agtcgtagtt atagaaa
//
LOCUS       DAEGIQ010000146         5336 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000146 DAEGIQ010000000
VERSION     DAEGIQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.152"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            16..1575
                     /locus_tag="KMT45_002616"
     CDS             16..1575
                     /locus_tag="KMT45_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBE7006045.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(1686..3617)
                     /locus_tag="KMT45_002617"
     CDS             complement(1686..3617)
                     /locus_tag="KMT45_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE7006046.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3635..4549)
                     /gene="pfkB"
                     /locus_tag="KMT45_002618"
     CDS             complement(3635..4549)
                     /gene="pfkB"
                     /locus_tag="KMT45_002618"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBE7006047.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(4546..5298)
                     /locus_tag="KMT45_002619"
     CDS             complement(4546..5298)
                     /locus_tag="KMT45_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE7006048.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         1438 a         1148 c         1015 g         1735 t
ORIGIN      
        1 aagcgaggcg tttgaatgga aaaaaaagga aaaatcaaga aattagataa gactcagata
       61 tattttattc taaaaggaat atttgttgga ctgctgacag gaatcgtcgt tagcctgttt
      121 cgattatcta tcgaaaagct aagcgattta gttcgaagta tctatgaaat gagtcgggaa
      181 cagccgattt acttaattgg cctagctatt tgctgcatcc ttgcagcatt tttcgtgggt
      241 tatctggtca aaaatgaacc ggatatcaag ggaagcggga ttccacaagt agagggacag
      301 cttcgcgggg aattatcaat gaactggttc tctgttttat ggaaaaagtt cattggcggt
      361 gtactttcag ttggtgcggg cctttttcta ggtagagaag gaccttctat acagcttggc
      421 gcatccattg gacaaggagc tggacaattg tttcggagtc catcttcaga agaaaagatt
      481 ttaatttcta gcggtgcaag tagcggactt gcagcagcat tcaatgctcc tatcgcagga
      541 ttactctttg ttttagaaga aatccatcac tccttttctc cacttgtttg gctgacatca
      601 tttgcttcag caatcactgc taacttcgtc tctctttact ttttcggctt gagacctgtt
      661 ttatatctag gtgaccttcc ttctttgccg ttgaaatatt atggttcctt agtgatctta
      721 ggagtacttc taggcatctt aggttttatt tatcagaagg tcttgctggc tttgccaaaa
      781 ttttacaacc gattgccttt accagcttat tttcacgggt tcgttccttt cttgctgatt
      841 ttgccaattg gttatttatg gcctagtact ctaggcggag gcaatcaaat cgttttagct
      901 tttggacatg cttccttgcc aatcattatg attatcggtt tgtttgttct tcgattcgtt
      961 ttttccatga tttcttacgg tggtggtctt ccagggggga ttttccttcc gatattgaca
     1021 ttgggggctt taatcggtag cttttatgga aacattatgg ttgatttcgg gatggatcca
     1081 atctacatca aggattttgt cgttattgcc atggctggtt atttcacagc gattggaaaa
     1141 gcaccgctga cagcaattat tttagtgaca gaaatggttg gctctcttaa tcatctgatg
     1201 cctttaggat tggctgctct tgttgcttat atcgtcaatg accttctcgg cggtaatcca
     1261 atttacgaat cattattgga acgtctactt ggaaatactc agaccgcctc actaaaaggt
     1321 gcaaggacaa cttttgattt tccagtaacg gcagagagtt ctttagacgg attaatggta
     1381 cgagacttca attggcctaa aaatatgctg ctaatctcta tacgcagagg aaatcaggaa
     1441 atcttgactc acggtgatac gatcatgaat gtaggagata ttttggttat cttgactgat
     1501 gaagcaaact tgccaagaat caagcaggaa atcgaggcta aatcttctat cacaaatttg
     1561 aagcaatcat tttaacgaac atggaaaaac tattttacaa caactattca tctacactac
     1621 ttgattctat atgattctat aaaaaaagaa gagactggga aaaaccagcc tcttcttttt
     1681 tatttctatg cttctgctgc tgcaggagct tttctcaata aaccataaat cacaccagat
     1741 acgactgctc cgatagcaat aaacaccaaa tacatgaccg ggtgactcag taagaataca
     1801 acgaaaatcc cgccatgagg agccagtaat ttgatcccga aagctccgac taatccgcca
     1861 gttaaagcag aaccaataat aaaacttgga atggcacgaa tcgggtcagc tgctgcaaat
     1921 ggaatcgatc cttccgtgac aaacgatagt cccatcacga tgttcgtcaa accagcatct
     1981 tgatctgttt tgctgaattt attcttaaac aaacgtgtag caacgaaaat tgccaatgga
     2041 ggtaccattc ctccagccat aacagaagcc atcacgacac ttccgccagt tgcaacagtc
     2101 gtagctaaag ttgctgtacc aaacacataa gcggctttgt tgattggacc gcctaaatct
     2161 gccgccatca ttccagctaa taatgcacca agtaaagcag cattcgaacc actcatactt
     2221 gtcaagaaac cgttcaatgc atcattgatt gctttcatag ggacattgac cagcaacatc
     2281 aagaaaccag taatcagtaa gccaaacact ggataaaaga ggatcgtttt aattccatcc
     2341 aatgattttg gtaagttctt gaataattta cgtaaaaata cgatgacata accagctaag
     2401 aatccaccaa tcagtgcacc taagaatcct gcaccgcctg tattagctaa tgcaccagca
     2461 gcaaatccaa cgatcaaacc agggcggtca ccgatacttg aagcaataaa tcctgctaat
     2521 accggcagca tgaaaccaaa tgcagcttgt ccgatttggt tgaaccagct agcagcttga
     2581 ttgtaattcc ctagatttga taattgatca tgtggcacgc caataaattg gtcgatcatg
     2641 aaagaaagcg caatagcgat tcctccacca ataacgaaag gtaacatgtg tgatacgccg
     2701 ttcatcaaat ctttgtaaat tctttgtccg atagtaccat ccgcatttcc ttcttctgaa
     2761 ctgctttctt ttccatcacc atggaatact ggtgcagtac cactaagggc tagattgatc
     2821 agctcttctg gtttacggat accatcactg acaggacgat tgatcagttc ttttccgtca
     2881 aagcgattca tctctacttt tttgtctgct gcaacgatta caccttctgc acgggcgatg
     2941 tcttcagcag tcaatcggtt tttgattcct tctgaaccgt tcgtttcgac tttgatctcc
     3001 acacccattt ctttcgcttt tttcttcaga gcatcttctg ccatgtaagt gtgagcgatc
     3061 cctgttggac aagctgtaac ggcaacaacg aattttcttt ctggatgagc agaagcttct
     3121 gcagcttgtg tttcttgttc tgctttttct tctgcttctt tttgctcttc agctgtttgg
     3181 aacaagtctt ggacttcttc tggtgtattt gcttctttta attttcctac gaaatctgca
     3241 tcaatcagca atcgagatag tgctgctaat gcttgcaaat gtgtgtcgtt tgcgccttct
     3301 ggagctgcga tcatgaaaaa cagataagtt ggctgtccgt ctaaggcttc ataatctact
     3361 cctttttggc tttttgcgaa taaaactgtt gcttctttaa ctgcactgtt tttcgcgtga
     3421 ggcatggcga ttccatcacc aagacctgta gaagtttgtg cttcgcgggc tagaattcct
     3481 tctttatatg tttcgatatc ggaaatccgg ccggcatcaa acattttttg gaccatttca
     3541 tcgattgcgc cttttttatc agtcgcttgc aaatccatga tcatcgcttc tttgaccatt
     3601 aaatccttga tattcatgat tatcctcctt gaaactaatc aatttttgtg atttcaactt
     3661 ctggcagcaa ctcatcaatc aattcgcgag ctgccaagtc atcagaaaaa gctgttgcgc
     3721 ttccgcaagc aactccccat ttaaaggctt cgattggatc tttcgtacgg ctgtaagccc
     3781 caataaagcc ggcaatcatt gaatcccctg cacctactga atttttcaat gggcgaacta
     3841 atacattcgt tttataagtt ccttgttccg tgaagagcaa agcaccgtct ccagccattg
     3901 agatcagcac attctgtgcc ccttcagcta gcaatttttt accatacgga atgatttctt
     3961 ctacagattg gaaggttgta ttgtaaagct cagctaattc gtgattgttt ggtttgatca
     4021 acaatggttt cttaacaagt gcatttttta ggtcatcacc tgttgtatca atcacgaaat
     4081 ctgcaccttt ttctcgaatg atatcaatca gttcttgata aaatcctttt cgcaggctag
     4141 ccggcgcgct tcctgacaat acgacgatat ctccttgtcc tactgttcct aatgcttgct
     4201 tcaactcatc gatttcttca tctgaaatag atgggcccaa accgttaatt tcagtttcag
     4261 tatctgattt tagtttgata ttgattcttg tatcttcttt tacggttgtg aacaaagact
     4321 ggatttgttc tttttcaagc cacttcgaaa taaattcgcc agtgaaccct cctaaaaacc
     4381 ctagtgctgt agattgagaa tcgatccgtt tcaaaatacg ggaaacattg atcccttttc
     4441 ctccaggtaa cttgaagtct tccttcattc ggttcaaatc gcctagtttt aacccatcta
     4501 cgcgtacgat ataatcaatt gatggattaa gtgttactgt gtaaatcatt gtattgcctc
     4561 cttaattgtt gtcttttcac ggaattcatt aacgaattct aatggacaac gctcggttat
     4621 gatcgtggct gcttccaaag gtgcaacaga ggtgaaggac aactggttga atttggtatg
     4681 atcaatcaat acaaatgtct cttcggaatt tttcatggct aaacgtttaa cagctgcctc
     4741 ttctgggtcc ggagtcgtga atccttgttc aagatgggca ccgttcattc ccataaatgc
     4801 tttattaaaa tgaaaatttt ttagttgttc tatcgcatta gatcctaaaa cagcattagt
     4861 tgccagcttt atcgttccac ctaaaataat ggtagcaagc tccaaatcaa ctaaatttgc
     4921 cgcatgttga acggaatttg tcactactgt gatctgtttt ccttgcagaa aaggaatcat
     4981 ttctaacgta gtagagccag cgtctaaata aatgacatct tcgtcattaa tggtttcagc
     5041 tgccgatttg gcgatttgct gtttttcgtg aacgtttttg agtgattttt cactcatatt
     5101 ctgctcaaaa ttcaataatt gctctttctt agctccgcca tgaactcttt ctagaaaccc
     5161 actattctcc agctcttgta aatcacgtct tatcgttgat tcagaagcat taaaaaggtt
     5221 tacaagatct tgtgatttca cgattttctg ctgttcaagc aagtctagga ttttttgtct
     5281 acgttcctct gtaagcattt tcacgatcac ctcaatacaa ataatagcat attttt
//
LOCUS       DAEGIQ010000147         5321 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000147 DAEGIQ010000000
VERSION     DAEGIQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.153"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            204..1079
                     /locus_tag="KMT45_002620"
     CDS             204..1079
                     /locus_tag="KMT45_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE7006049.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1183..1947
                     /locus_tag="KMT45_002621"
     CDS             1183..1947
                     /locus_tag="KMT45_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBE7006050.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1934..3376
                     /locus_tag="KMT45_002622"
     CDS             1934..3376
                     /locus_tag="KMT45_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBE7006051.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3387..4717
                     /locus_tag="KMT45_002623"
                     /pseudo
     CDS             3387..4717
                     /locus_tag="KMT45_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            4730..5188
                     /locus_tag="KMT45_002624"
     CDS             4730..5188
                     /locus_tag="KMT45_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE7006052.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1805 a          774 c         1112 g         1630 t
ORIGIN      
        1 ttttatgtaa ttacctccaa atcaattcga tacaagtatt atcataaaac gagtaaaaaa
       61 gatattgata acgcttttat tatgtgttaa actatttttg taaattatgt agggtgctac
      121 tattcaatta ggcacgacct aagaaagggt tctttcttag gtcgtttttt gtctttaaaa
      181 attgtttgga gacaaaaata tgtatgaagg agtctgcaag ttgttttgtt ttatcatata
      241 gtggaggtga attaacggtg tatgtgatga aaaagctgaa tcataatgct gtattgatcg
      301 atgacgatgg aatagaaaaa attgcgatgg ggaaagggat tggcttttct gctaaagtga
      361 gtaagccatt tgatgttgag gatgctgaca aattatttgt attagattct aaagaaaaga
      421 tcaaacgatt cagtgagata gtctcgcaga ttccaatcga atttatggat tttgcggaag
      481 aagtgattga actgataaca gctaggatct cctctccatt agattcaaat atctatatta
      541 cattgacaga tcatatttat tttgctattc aacgaaaaag agaagataga gatgtcacag
      601 caattatgtt gccagaaatg agattccttt atccaaatga atatgatata gcaatagaag
      661 tggtcgagcg aatcaatcat cgatatcaaa ccgagttagg gtcaaatgaa gtaggattca
      721 tcactatgca tatcattaat gctgagctag gagagcctaa tagtttaaag agcttaaaaa
      781 tagtagaaat gacgaccacg attttgtccc atctagaaaa atcctattca aacgtttatg
      841 ataaagaatc actgacttat catcgcttaa tggttcatat caaattcctg gttaaacgct
      901 tgatttatca ggaagaaacg ccagtagaaa atattggttt ttttactgaa acatttaaac
      961 atagcaaagc gtattgtaca gcactaaaga ttaaagatat catcgaacag caatttcaac
     1021 ttgccattca agaaaatgaa atcatctatt tggcaattca tctagcacga atctattaaa
     1081 ataagggcgt aactatctat aggcgcaacc aaatgacaca tagtaaatgg actatgatta
     1141 tttggttgcg cctattttag tagaaaaaag aaggagaatg aaatgggaaa agtgattttt
     1201 aattcggatg attttggcta tagccatggt gtgaattatg ggattatgga tgcttatcag
     1261 cgaggaattt tgacatcaac tacattgatg gcaaatatgc cgggtttcga gcacgctgtg
     1321 aaactaagaa aggaaatgcc taggctaggt gtaggagtcc atttaacatt gacgtgtggc
     1381 aaaccacttc tcaaaactgt cgatactctt atcgaaagcg gagagttcag atccttatct
     1441 tattatcagc atccatttaa cattgacaaa gatcaactgt atcaggaatg gaatgctcag
     1501 attcaaaaaa tttatcatgc ggggatcata ccaactcatt tagacagtca tcatcatact
     1561 catactttgg atgaaaacca ggaggtagtt gttacattag caaaaaaata tgatttgccg
     1621 gttcggagca actttgaacg aaaagatgaa gttcgtcatg tcgattattt tgagccttgt
     1681 ttcgatgtcg taggagaaac tgatgaaaaa aaacgacaaa tcaatacgag tgtggaagaa
     1741 tatttggcaa gtttactcat gatattgaga gaagataaaa cgatcgaagt catgtgtcac
     1801 gcagcgtatt tggatcagga attgttaaat ggttcaagtt ttgtgtatcc tcgtactcac
     1861 caagtcgaat ttttaattca ttcagcattt gctcaaaaag tgaaaaatga tcctttgatt
     1921 gagttagtaa catatgagga tatttaatag aggaggaata aaaatgaatg ttaaatcaaa
     1981 tgtgcagaag cttggaagag cgatgctact tccagttgca gcgatgccat tagcgggatt
     2041 gatcatgcgt ttatcagctg atgatatgct gaacatccca gtaattggtg cagctggaaa
     2101 tgccgttttt ggaaacttgg atatcttatt tgctatagga gttgcaatcg gttttgtcaa
     2161 aaataaggac aaaggaatac ctgcacttac tggtttttta gcgattgcta ccttgaaaaa
     2221 aggcttagaa atcatggacc cagaagtgaa tatggggatt tttggtggaa ttatttccgg
     2281 tttagtagcg gcatggacgt ataatcgctt taaagaacaa aaattaccag tagttttttc
     2341 ttattttgcg ggcgaaaaat tcccattgac tatggtgatg attctacaaa caatcgcagc
     2401 tatttttttg ggctttatct ggccgctcat ccaaattggg attgataatt ttgcaaaact
     2461 gctcgttgat atgggggcat taggtgtggg gatatttatg tttttgaatc gtttgctgat
     2521 cccctttgga ctgcaccatg tgttgaatac gtatatttat tacgatttag gaagctatac
     2581 atctcctagc aaggaagttt tcagaggaga gatgacacgc tttatcaacg gagatcctca
     2641 ggctggactg tttttatcag gtttttttgt agtaatgatg tttggtatac cagctatatg
     2701 tctagctatc tataaagcag cttataaaga aaataaagag atggtcaaag gaatcatggg
     2761 aagcggtgca ggaacttcat ttattgcaaa tatcactgag cctgttgaat ttagtttcat
     2821 gtttctttct cctatgttgt acgtgatcca tgcacttttt gccggtttag cagggattgt
     2881 ctgttactta ttcaatatac gaattggttt tacattcgga gcatgtattg ttgattatct
     2941 catcaatttt cgaatcgcta ccaatgctat tttgattctt ccaatcggaa ttttcttttt
     3001 tgccttgtat tacgttactt tttattattt gatcaataag agaaacattc agacattagg
     3061 acgtgaagct aaggcagagt ttggcaatga agtaacctta gaagaaacag agttagggtt
     3121 agcaagtaaa aattatgaat atatggcaac aaaaatgcta caagcgtttg gaggaaaagc
     3181 caatatcctg gatgtctata gttgtaatac acgcctacgg gtagaggtag tggatcccac
     3241 catggtcgag gaacagcgaa tcaagcaatt agggataagc gggatcatta aaccaactga
     3301 aaaaaattat caaattatca tcggcttaga agtgacttat gttatggcag aattcaataa
     3361 attattagaa gaatagaaag gaaaagatga tgatgaaaaa acagattatc actatagctg
     3421 gaggcgggag tacgtataca ccaggaatcg ttcaagccat tttgaacaat gaaaaacggt
     3481 tgccattatc tgaaatacgt ttatatgata ttgatgaaga aagaaatatg gatatgtact
     3541 tgattgttaa atttatgctg aaaaaaaagg tttttcaaat attcgcattc gtgcaacgga
     3601 tgatccaaag cttgctttta caggatgcga ttttgttttc tcacaaattc gtgttggagg
     3661 attggagatg cgagaaaagg acgaaaagat tccacttaaa catggattag taggacagga
     3721 aacgtgtgga ctcggaggat ttgcttatgg gatgcgctca atcaaaggtt tattggaaat
     3781 tgtcgatcat atacaagact atgcccccaa agcatggatt ttgaattata caaatccaga
     3841 gtcgatcgtt tctgaagctg ttcgtcgtaa atatccgaat gtaaaaatga ttaatgcctg
     3901 tgatatgact atttcaattg aagaaacgat tgcagtgaat tacggttatg atcgtaagaa
     3961 ttggatcgtt acgtattatg gtttaaacca ctttggctgg tatacatcga tttatgataa
     4021 agaattgcaa cgagaaatta tgccagagat tattgaaaaa ctaataacaa aagaaatgca
     4081 agtcgccgat tttaatatag gagacaaaac ttggcaaaaa acctttcaaa tgatgtcagt
     4141 catcacgaaa aattttccaa gtaatatccc gaataattac ttagagtatt acttatatcc
     4201 tgatatggtc gttgagcata ctgacaaaga gtatacacgt gctaatatgg tcatggatgg
     4261 acgagagaaa aataccaaag aaatggctga taaaatacgc cgcggtattg tggaagaagt
     4321 attgaacttt aattttgggg agcatggaca atatatcgtc gatatagcta tttccttgtt
     4381 gaatgatgat cgtcgtcgct ttatgctaat cgttcctaat caaggggcga ttccgaatct
     4441 acggtcagat gcagttgttg aaataccggc atatgttggt gcaactggtg tggagacaat
     4501 tacactaaga aaaccaatca gtgattttca taaaggattg atggaggcac aagttgcagc
     4561 agagaagtta ctagtagatg cttactttga aggttcttat caaaaagctt tacaagcatt
     4621 tacattgaat caaaccgtac caaatgccag agtcgcaaaa aaaatactag atgaaatgat
     4681 caaagtaaac aaaaaatatt ggccagcatt aaagtagagg tgataaaaag tgcatttctt
     4741 taaaacaaga aacattctaa aagctgtagc tagtggtcaa attattccat taacagcagt
     4801 aaaagacgag gtcttttctt caaaaatgat aggagttgga tacgctataa cgaatcatga
     4861 tggatatatc catgcgccag ttaaaggaat aatcgataat atatttccta cccgacatgc
     4921 cataacgatc gaaagtgaat ctggcagaaa aatactgatc catatgggtt tagatacagt
     4981 tgaactcgaa ggaagtccgt ttcagatacg ggttgaaagt aaccaggagg taaaagaagg
     5041 gacactgtta gctattatag atttgaagaa gttggagcaa gcacaaaaag atacggtgat
     5101 cattgtagtt tctccaggca tttttgaagg aaatttatta aaacaaaacc aagaagtaaa
     5161 gattaaagat agttgttttg aattttagtg tacgatgaaa aaagttaaat agggtgatat
     5221 ttatgggtga atttacaaaa tttttttgaa atgataagtt tcaatcacta gatttaatca
     5281 ttttgatttt tgttaattat cgaatttttt tgttgctcct a
//
LOCUS       DAEGIQ010000148         5253 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000148 DAEGIQ010000000
VERSION     DAEGIQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.154"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            55..843
                     /locus_tag="KMT45_002625"
     CDS             55..843
                     /locus_tag="KMT45_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBE7006053.1"
                     /translation="MVLLESMPDGYIYSNILLKLYLRSLKHEGKLMFNDRIPFNSTML
                     ATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDIQSFIGKSSTEADRKRKYRKEI
                     EEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEKDIENVTPSKKSKAKPVRHKYG
                     EYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSE
                     SKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            855..1124
                     /locus_tag="KMT45_002626"
     CDS             855..1124
                     /locus_tag="KMT45_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006054.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1129..1293
                     /locus_tag="KMT45_002627"
     CDS             1129..1293
                     /locus_tag="KMT45_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006055.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1286..1588
                     /locus_tag="KMT45_002628"
     CDS             1286..1588
                     /locus_tag="KMT45_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006056.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1585..1746
                     /locus_tag="KMT45_002629"
     CDS             1585..1746
                     /locus_tag="KMT45_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBE7006057.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1743..2048
                     /locus_tag="KMT45_002630"
     CDS             1743..2048
                     /locus_tag="KMT45_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006058.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2048..2404
                     /locus_tag="KMT45_002631"
     CDS             2048..2404
                     /locus_tag="KMT45_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBE7006059.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2391..2609
                     /locus_tag="KMT45_002632"
     CDS             2391..2609
                     /locus_tag="KMT45_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006060.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2606..3025
                     /locus_tag="KMT45_002633"
     CDS             2606..3025
                     /locus_tag="KMT45_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HBE7006061.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3022..3579
                     /locus_tag="KMT45_002634"
                     /pseudo
     CDS             3022..3579
                     /locus_tag="KMT45_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
     gene            3576..3872
                     /locus_tag="KMT45_002635"
     CDS             3576..3872
                     /locus_tag="KMT45_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006062.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3949..4362
                     /locus_tag="KMT45_002636"
     CDS             3949..4362
                     /locus_tag="KMT45_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBE7006063.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            4671..4823
                     /locus_tag="KMT45_002637"
     CDS             4671..4823
                     /locus_tag="KMT45_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006064.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(4820..5095)
                     /locus_tag="KMT45_002638"
     CDS             complement(4820..5095)
                     /locus_tag="KMT45_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006065.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
BASE COUNT         1928 a          800 c         1158 g         1367 t
ORIGIN      
        1 aacaaacgat actactattt aaaactaaaa gagaactttt tcgatagcga cgagatggtt
       61 cttttagaaa gtatgccaga tggatacatt tattctaata ttcttctcaa actttattta
      121 agaagtctga aacatgaagg caaactaatg tttaatgaca ggattccatt caattctaca
      181 atgcttgcga ctattacaag acactctgtg ggagttgtag aaaaagcagt acaaatattt
      241 cgtgatttac aacttattga ggtattagat aacggagcaa tttatatgtc tgatatacaa
      301 agttttattg ggaaatcttc aactgaagct gataggaaga gaaaatacag aaaagaaatt
      361 gaggaagcaa aacagaattt aataacaagt ggacaaatgt cggacaaatg tccggacaaa
      421 actacaccag agttagagat agagttagag aaagatatag agttagagaa agatatagag
      481 aatgtaacgc cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata cggagagtat
      541 aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaatagaatt ccctaatgac
      601 taccaagagc gaatagaacg gctatctgag tattgtgaat catctggtaa gacttataaa
      661 aactatttgg caactattcg aagctgggca agaaaagaaa aaagtgaatc taagaacgca
      721 agcagtggat acaagcgcac aggaagacga gagaagcttc ctgagtgggc aatcgaccaa
      781 gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa agcaccattc
      841 taagaggtgg gaaattgaaa atcgattatc tagaactaat taatgaaata gcaaagtata
      901 aaactggtga ggaaatagaa atcctgagag acgtatatga tcaactcgaa gaagctggaa
      961 tcgaaggaat taagaatgat cgttcgagtt ggagtaagct cagatactat ttcgcacttt
     1021 atatcgatac aacacaatta agaaatttag catataccaa attactattt gttgattgtg
     1081 ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaatcagat ggacctaaag
     1141 acatttacag cacagattga actaatgcat caagaagctt taagacaaag tgtatcgtac
     1201 gaagacaagt ggctcaacac gttccacggc ggacgtgaga gcgcacttga tcaagtactc
     1261 aaattactga aaggagaatg tcgggatgga taagaaagcg gcaatgcagc gaattatcga
     1321 attgacttat tcagaagatt ggcaaaatga caaagaagct gcttcagaag tgatgaggct
     1381 tggaagagcg atgtgggcag acaagagcaa caagccaaga ccacgaaaaa tcgcaatttg
     1441 gcacggtgac aaacttctag tgatagggac agctgaacag ttagcaagtc tcacaggctt
     1501 gcacgagaaa atcgtgagga aaagagcaag gtgtggctac acagacgcta agaagagaac
     1561 gtttagatac gtggaggaat catcatgaca acagaagaag tgattcaaat gcgtattcga
     1621 agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg
     1681 gcgagaaagg caatcgattt gcacatagag aatttaagaa gggaaatcca tcgattggag
     1741 gaatgagcgt ggataagaaa gcagcaatga aaagaattgc tgaattaacc aagtcagaat
     1801 cttggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga
     1861 ctgaaaagcc caaacggaga acgccgagaa aaattgcaat ctggcatgat gaccgaattt
     1921 tagtaacagg tactgctgaa cagttatctg aaattactgg attaagcaaa aacattatct
     1981 gggatagagc taggagctta tggattgatt caaaaggacg acagtttagg tatgtggagg
     2041 agagataatg gatctcatta cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa
     2101 gattcagaaa gataaaaaat caaaagaacg agctgaatta gttaagttag aaatggctga
     2161 aacaggagca ggagtgcgaa gtagcaggca ttggaaagca gcagcaaaca ttgaatttta
     2221 ttacaacgaa attcaaaaag ggttcgatca gatgcgtgag ctggatcggc aaacaaattg
     2281 gagcaagaaa cttcatcaag atcgtttcaa atttgtagag aagtataaag aaatattaga
     2341 agagtatttg aggaggacag caaatgataa aaaaactcgt tcaattcagc atggatttat
     2401 atgatatcga atcaggagca acactatctg tggaatcgga ccatctaatc ataaattttg
     2461 gtggaaagcg ccagattatt ttgtgggtag ttgatgatgt actgtttcca gaaattgttc
     2521 atgatttcga agaatcaaaa gcggttgagt ttgaaatagt gaaaaaagta atggaattga
     2581 ttgaaaaata cgaggaggac agcgaatgat accgaagttt agagcgtggg ataaacgaaa
     2641 gaacgtaatg agagatgtag ccgtcttgca ttttactaaa aacggcaaaa caaactttat
     2701 tgaatattgg ataaatccta ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat
     2761 gcaatccaca ggaatgaaag ataagaatgg tgtggatata tttgaagggg atatagtatt
     2821 agtcagcgtg cgaaatggct tcgattactt agataataaa gtctgtattg tcaaaaattc
     2881 aatagattat tccggattag tttgtgccac tgttgatgaa gacttagagt atcaaatttt
     2941 taacacagag ctgtttgaag aatacacgta cgaagtcacc ggaaatatat acgagaatag
     3001 cgagttattg gaggaacagc gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt
     3061 aaagcgatag gaaatgatgc aatagctgct tgctataacg agagtataaa ttcaggtatt
     3121 acgttaatga aaaaaataga cgaaccgcag aaaccagtta ttccacaact cgtggccggt
     3181 tggcttgaga aatctacgga cccttttaca aaagctgaaa aaatagcgta tttaatcaaa
     3241 tctaaagatg gtgattcata ttatttctgt gattggtttg tacgagatgg catagtgacg
     3301 caagagcaag gagaagaatt actcgcttgg gcaacaagac aatcatatga aacactattg
     3361 agcctataca acggctaaga ggttgagaaa gagccgttat gggcaataaa gaatgccgat
     3421 ggaaactatc ttactaaatg tgctttatgg ggaaaagatg gagtaaatta tagttttgaa
     3481 tgcaatccat ctcatcgatt gcttttcact gataaagcaa cagcggatgc tgcagcattg
     3541 ttggtgaatg gaacagtgga agaggtggta gaaagatgaa actaaaagac ggattttacg
     3601 ctagcagtca cggtatcggc ggtttaatgc tagatatgcc gacaaagaac cctaaaacac
     3661 gcaagaaatc aaaattcaaa gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt
     3721 atccgttcag aggatacgtt aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa
     3781 atacaatgaa gtgtgatcgt tcgactgcaa aacacaagca ttatttagct gtagcgagat
     3841 tagttgacat ggaagtaatc aagagcaaat aaaaaaagcc ggatcgctcc gactgattca
     3901 ataaatccaa cacatttatt atatcacata aaggagcggt ttgacttgat gcaattgtta
     3961 cgagaggtag atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg
     4021 cgtttggagc ggatggcagg tcgctctttg atagatatta agtcgccgat tataacggat
     4081 atgccgaagg caccgaagca tggcaataag gcagaagacg cgattattca gatgatggat
     4141 atagaaacgg agagagatgc gattctagcg gctttgatgg ctcttagtct gattagtcgt
     4201 cagatactct actacagctt ctgtgtgcca gatagcttct caaactacag aattagccgt
     4261 gaagtgggtt attcagaaag aagtatacaa cggatgaagt cggaagctct aatagagttt
     4321 gcagaagcat ataaacacgg aaaaataatt gcttataaat aatttggcgg ttttttggcg
     4381 gaatgatggc ggtttttaga catttaccag tgttattatg gtagtgtcga aagattagga
     4441 aacaggatcg acaaaataaa cgaagggagg caatctccct catcgtttta aacttcttct
     4501 tagatagaca gcaactaatt atgaagaaaa ggatgtgaat tcaactcctc ctaaattctt
     4561 catcggttgc tgtttatttt gattagacag cagcactcac aaaactaaac atgttcttgt
     4621 cgtctttcaa ctgctgctgt ctattaattt atgtattgga gggaagaaaa atgaaattat
     4681 cagtagaagg cactccagaa gaaatacaag aattgctcca agctattggt agtagcaagg
     4741 agcaaaaggt aagtgtttct aattcagata ttgacggtat ttgcaattgt ttaaacagag
     4801 aaaaaatatc taatccttac taagagaaaa tatgttcgtt aatgaatttc caattttcct
     4861 ctgtagctct accttgataa tttttttgca gttcacagat gaataaggaa tcgttattat
     4921 caagtactgt atttaatttt tcacacattt gatttggatt caaattagtt ttaactaacc
     4981 aaaaagattt ttgtagcttt atatatgccc caaattcttt tattatttca aatactttat
     5041 catacttttg ccctgggtta ttaagatcat aactaatcat gtagggttta tacatgttgt
     5101 cttgcacatc cttgttttat atttcagcgg accactcgct gataaataaa attatacgct
     5161 tagtatttat tttcacaata ttaatttatc gtaataactt ttgagtaaat agaacaaaaa
     5221 acctgcacta gtttccgcta gtacaggcag tga
//
LOCUS       DAEGIQ010000149         5213 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000149 DAEGIQ010000000
VERSION     DAEGIQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.155"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            604..954
                     /locus_tag="KMT45_002639"
                     /pseudo
     CDS             604..954
                     /locus_tag="KMT45_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
     gene            948..1370
                     /locus_tag="KMT45_002640"
     CDS             948..1370
                     /locus_tag="KMT45_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006066.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2541..3509
                     /locus_tag="KMT45_002641"
     CDS             2541..3509
                     /locus_tag="KMT45_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBE7006067.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3526..4050
                     /locus_tag="KMT45_002642"
     CDS             3526..4050
                     /locus_tag="KMT45_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBE7006068.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4426..4707
                     /locus_tag="KMT45_002643"
     CDS             4426..4707
                     /locus_tag="KMT45_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006069.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4709..5125
                     /locus_tag="KMT45_002644"
     CDS             4709..5125
                     /locus_tag="KMT45_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006070.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1967 a          893 c          868 g         1485 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct taaaaaaatg acagataaaa atgcacaaag aattagatat agataaccgc
      301 gaaaaatcaa gctttagaaa gttgctttta gctagaccaa ttaaaaatca caaagtgagc
      361 gtctctatcc attggtagag gggctacttg gcatagtgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaagcagttt atttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggattttat actaagactg
     2041 tcttaaaacg tctcaaacga aggctcatag cttctcaaag agttccttat gaacattttt
     2101 agcaaaggag ccggaagcga ctgcgcagta taatgtgaga gcagagcgat cacacctttt
     2161 taagcgactg tagggagtaa gattcccagt tgagaaaaat gcgcgaatct gtttggagaa
     2221 aaaagaagtg gttttttagg cgggaaattt tgtccccctc ttttataatc tctttttaaa
     2281 cctcttttta aacctcttta ccgtcggcta tatccttact ctcccaaggg aatgacgctt
     2341 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2401 gtctgtttat ctacatcgtt ttgtctgttt gtacattgaa ttagtacatc gacgatgtta
     2461 atataagatg tagataaaca ggcattttgt atttcataac gtgtatctct tgtgcgagta
     2521 agaaaggagc tattttaaat atgtctaatg aaatcgtaaa gtatcatcat gaattaaata
     2581 caattccgtt gcgaaaattt acaccagtcg aaatgaattt gttcttttct atcgtatcaa
     2641 gaatgcgtga tgtaggtgat aaaaaagtac aatttacatt cgagcaatta aaagatttaa
     2701 gcaattataa agcaactgca aatgttcgat tcatagatga tttagaaaca acgtatgaca
     2761 aattaatgga tttacgtttt ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg
     2821 ttttatttaa tcaatttaaa atcgacggaa aatctgatat tccttttgct gaaatacaag
     2881 ttcatgaaaa agccttaccc ttacttaata atcttgaaga atgggtgcgc tattctctcc
     2941 aacaattcaa tgaattagaa agcagttatt caaaaacaat gtttcgatta ctaaaacaat
     3001 acagaaccaa aggtttcgct tatttttcaa aagaagactt tcatgaatta cttgatattc
     3061 ctaaaagtta caaccaaggt acgattgatc aacgtgtgtt caatccaata cgacaagaac
     3121 taaccgcaat ctttaaaggt ttaacaatca aaaagaaata cggaaaagga cggggaaagc
     3181 ctgtcattgg ctatcaattt acatttaaac ctgagataaa aaaatcagac gactttaaca
     3241 aacatgcccc aacaaaagaa gaacaaaaca aagcattaga actagctaac caacaagttc
     3301 aatctgaaaa agcacctact tcattacaag aaaaaatcaa agaacaaaag cgaaaattgg
     3361 aacttttaca acagttagaa aagttagaaa gagaacaaaa tacccaaaat aaagaaaata
     3421 atgcacaaaa atcagagata aattccgata ttccttcaga actaaaaaaa gacttattag
     3481 acaatcttca aaacttattc aaagattgag gggtttttag attttatgac agaaaaaaca
     3541 atcaaagaac ttgctgaaga attaggagtt agtaaaacag caattaacaa aaaagtttct
     3601 gacgaaaatc gaaaactttg gttttcgaaa atcggaaaca agtttgtgat taatgaaacc
     3661 ggacaaaagg ctataaaatc aatgtttttt aacgaaaacg aaaactcaaa tcaacaacta
     3721 gttggcgaaa aaacgaaaac cgaaacccac aaaaacgcca accaagttcc cgaattagaa
     3781 gtcctttctc tcctagaaca acagttaaat caaaaagata tgcaactaca agaaaaagac
     3841 gagcaaataa aacaaatgca aaaattactc gaccaccaac aacaactcac cctgcaagcc
     3901 aaccaacaaa tcgagaaact gcaggaacaa ctacagttga cttatacgga agaatcttcg
     3961 gaaaataaat caacgactct ttctgaagaa acagaatcga tagaaaaaca atcaaagacg
     4021 gagaaaaaat ggtggcaatt ctggcaataa aaccagcaat ttaaattaat aaaaattgct
     4081 ggttttattc ttctttttgc ttttgcgagc agagcaacat ggacccatgt tgcgatattt
     4141 tggtggcgcc ccaatccccg ttttttcgtt gaatcttact cggactgcac tcgttttaca
     4201 gctgattttt ctgaattttg aaagagcata aggaaacgtg gtatcacttt cttacgaaag
     4261 gtgctagata ggctgtgcct atcacgtttc tcagaaaaaa atttgctatc acttttttgc
     4321 cattgtggct aggctatgta tagtgttttg gcgaaaaata gctatacaaa tactatattt
     4381 gtatggtata cataaattat acgctgatac gaaaggaaaa tttccatgga cattttagtt
     4441 cgaaatatca atcccaaaat tattgcaaga atagataaac gttgtcagga actcacaacc
     4501 aaaacgggca aaaaatggag tcgaaataaa tatctaaaag ttctaatcga aagtgatttt
     4561 gaccgtcaac ttatgaatta caaaaaagat caattcgatt tattactcga aaaatttatt
     4621 gccattcaaa cagaaaatac gaacatactg aatgactatg ttcacacaaa caaccaactc
     4681 attgaaacac ttatcggagg aatataacat gagaaaaact tctgttcaaa aacacgtaac
     4741 cctttatccc agtcacgttg acgttttaga ttccatcatc gaaaaaaatc cagacgtcaa
     4801 caattattca gaagccattc gttttttatg tatgagttat gaacctgata ccaatacaac
     4861 cacaaccagt aaccaagcaa aactcaatgc catgggaaaa gaaatttcta tcatgacaga
     4921 aatcatggca gaatttgctg atttatatct aaaagatact ggtgttctta tcggtacaaa
     4981 ttctaaagtg tatcaagaag tgaaagaaaa aattgaaaat caactcaatc cttcccgacg
     5041 aaatcaaccc aaaaatcgtc aaaaagaaac caatcaaacc tttacttctg gaaattttga
     5101 cccaacaaac tttctaaaaa gataaaaaaa gaaaccgatc cctaaaaaaa ttcctcgatt
     5161 tctttttagg gatcagtctt ccagcaagat ttgcaagttg gtaccaactt gcg
//
LOCUS       DAEGIQ010000150         5189 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000150 DAEGIQ010000000
VERSION     DAEGIQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.156"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            137..748
                     /locus_tag="KMT45_002645"
     CDS             137..748
                     /locus_tag="KMT45_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBE7006071.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="KMT45_002646"
     CDS             821..1750
                     /locus_tag="KMT45_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBE7006072.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="KMT45_002647"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="KMT45_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="KMT45_002648"
     CDS             complement(2693..3871)
                     /locus_tag="KMT45_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBE7006073.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3894..4067
                     /locus_tag="KMT45_002649"
     CDS             3894..4067
                     /locus_tag="KMT45_002649"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006074.1"
                     /translation="MCCGNSILLKGLPFFYLHKIFYTLGLKERGLSGVKMVISDAHIG
                     LTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="KMT45_002650"
     CDS             4098..4499
                     /locus_tag="KMT45_002650"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006075.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5189)
                     /locus_tag="KMT45_002651"
     CDS             complement(4706..>5189)
                     /locus_tag="KMT45_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBE7006076.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAEGIQ010000151         5179 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000151 DAEGIQ010000000
VERSION     DAEGIQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.157"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(139..1101)
                     /locus_tag="KMT45_002652"
     CDS             complement(139..1101)
                     /locus_tag="KMT45_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7006077.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1103..2542)
                     /locus_tag="KMT45_002653"
     CDS             complement(1103..2542)
                     /locus_tag="KMT45_002653"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBE7006078.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2727..4673
                     /locus_tag="KMT45_002654"
     CDS             2727..4673
                     /locus_tag="KMT45_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBE7006079.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4937..>5179
                     /locus_tag="KMT45_002655"
     CDS             4937..>5179
                     /locus_tag="KMT45_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBE7006080.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1669 a          878 c          855 g         1777 t
ORIGIN      
        1 ggatttctcc actccaaaaa agtctaactt ttgggggtca ctacaagttg acaactcctt
       61 tttgtttaca aatcaattgg atatctacaa attgtatttt ctaacgaatt ttatagacat
      121 taaaaaatta atttaacgtt aaatacttcc tccagaaagt attgagattg gtaaaatata
      181 atccttattt gtcttttcgc ctttaatttt tttcagaaga atatcaacaa taagacttgc
      241 aatttcatca attggttgtc ttattgttgt caattgtggg aaaaatttct caataaaaga
      301 ggtaccatcg tatccaataa tttttaaatc ttctgggata ttaagtttaa gttgatgagc
      361 aattttcata gtgagaatgg ctgttaaatc atcagaaaca aaaataccat ctggtttgtg
      421 atttataata gttgacttta tttccatttc acgacggata gtagacagat tattaggaat
      481 tttgaaaatt tttccatttg gattttgaaa agaaaaacct agtgcacgta attctgtggg
      541 agaatccgtg ttatcatttc ctgtaatcat aatggtattt tgacaaccat ttttttgtaa
      601 agtcttagct gcaagttttc ccccttcaaa gttatctgaa gaaacaatgg aaacattagg
      661 agctaaattt ctatcaaaag caacaatagg tgctctcaca cgctcataat cagcaattcc
      721 aagattatgg cttgatgaga ttatgccatc aacttggttg gcttcaagca tttcaatgta
      781 ttctctctct ttagcaggat cattttgact attgcaaata atcgttttat atccatgttt
      841 aaataactct atttcgagat actcaatgag ttcagagtaa aagatattac taatattagg
      901 aaaaataaga ccaataagct gaggtgactt tccttgaagt ccacgtgcta aattatttgg
      961 tttataacca agagttttca tagcttcatg aactttttgt tttgtttttt ctgataaata
     1021 tcccttatta ttaatgacgc gtgaaacggt tgttgggcta actccagcta attcagcgac
     1081 atcagttaat tttgctacca ttttaatatt ttaattcaaa gtattttcca gtaggttcgc
     1141 ctgatttgat ttgaatacca ttttggtgat tttcagggaa tacacgacta gtaaacactt
     1201 tttctccttt attaataaaa atctcaatga ttgagttatc tacaaaaatg ttagcagttg
     1261 tttctttagt atcaattgag cattcacgaa tcgtaccaaa gtcagtggca tattgaatgc
     1321 ctgctttgct gcgatctaaa ataatttttc cttctttggt atctacaatc aagctaagtc
     1381 catttccttt actatcagag aaaagaagta actccgattt ctgatttgga ggaaaagtta
     1441 gttcaagctc ataagtatta tttgtttctg cttttgctgt aaattgttca gaactagcac
     1501 gtaatgatgt aatagcttct actggatatt ggtaaagttt accatctttt attgaaagtt
     1561 cttttacaag actcattgct ccctgataat catacttatc agttggataa tcgatatcag
     1621 gtagtccaat ccaactgaca atcagagcac gaccatcagg agtattgaat ccttgagtcg
     1681 catacgcttc aaatccataa tcaagatttt gaatttctga agcgcccaca agttttactt
     1741 tttctatgtc aagcgattga cagatcttaa aagtattcgg atagatattc ccataattta
     1801 gctcagattt atccaaccct tgaggacaat aaatgagaac aggttttttg tctacaaata
     1861 ctagatttgg acactcaatc atgtattctg aaccagtgcc gccaaaatca aggttgccaa
     1921 cttcctccca tgtttccacg ttattgtcat ccgctttgta aagtttgata aagcctgatt
     1981 tttctaaatt ttgtgctcca acaatcgcat acaattgtcc ttcataatta aacatttgtg
     2041 gatcacgaaa atgatcagtt acatcagcag gtttatgaat aagaacattc tcacctttcg
     2101 taatgttgta gtctttatcc atccatgctc cgatttgaag tgtatcacgt tcccaatttt
     2161 catttcgaac gtttcctgta tagagtaaga atagattccc ctctatttca tatgcagaac
     2221 cggaataagc gccgtggcta tctcgttttg tgtctggaag aagttctgct cctgttacat
     2281 gaaaatgaac taaatcatct gattcattgt gtacccattc tttcaaccca tgagcagctc
     2341 caaatggcca attttgataa aaaagcgtat attttccgtt aaaaaaagaa aaaccattag
     2401 gatcgtttaa caatcctgtt ttaggttcga tatgatagct agcacgccaa ggtgatcgtt
     2461 ttacattatc cttaatttta tccatctcat ctttagtcca atcagaataa gcacgatatc
     2521 gtatttctcg aggtaagttc atattttact ccttcaattt atctttttac aactagtatg
     2581 gtaaacgttt tccgttgaaa aatcaatcat ttcatttaaa aaaataaact atatgacaaa
     2641 cgtttgacat atttttaaga tatgctaaaa taaatttgca tttcagtgaa ccgttttcac
     2701 gtaaaaataa aaaaggagtg attcgaatga attacagtaa agtagcttcc gacgtaatac
     2761 aagcggtcgg aaaagataac cttattgcag ctgcacactg cgctacacgt cttcgtctcg
     2821 tactaaaaga tgacacgaaa gtgaatcaaa aagctttaga cgaaaatcca gatgtgaaaa
     2881 gcacatttaa aattaatgga caatatcaag tgattatcgg tgcaggtgat gttaatttcg
     2941 tttatgacga acttatcaaa aaaactggac tttcggaaat ttcaacagat gatttaaaac
     3001 aaattgctag taaagataaa aaattcaacc ccattatggc attaattaaa ttattatcag
     3061 atatttttgt tccaattata cctgcccttg ttgcaggtgg tttattaatg gcattacaaa
     3121 actttttaac agctgctgga ctttttggac caaaatcaat tgaagaaatg tatcctgcga
     3181 ttgaaggtat ttcctcaatg atccaactga tgtccgctgc accattcatg ttcttaccaa
     3241 ttttagtagg tatttcagct gctaaacgtt ttggagcaaa ccaatttctt ggtgctgcaa
     3301 ttggtatgat catgacaaca ccagatcttg gtggctcaac tgaatattgg aatatttttg
     3361 gttatcatgt ttcacaaaca aactatgctt atcaagttat tccagtttta gcagctgttt
     3421 ggatattatc tatattagaa aaattctttc ataaaaaatt gccatcatct attgacttta
     3481 cattcacgcc attattatca gtaatgatta ctggattttt gactttcaca gttattggcc
     3541 ctgtaatgtt attgttgtct aatggaatta cagacattat tgtttggcta tataacacta
     3601 ctggatttat tggaatgggt atttttggag gaacatattc acttatagtc atgactggtt
     3661 tacatcaatc atttccagct attgaaactc aattactttc agcgtggaca aatggtatcg
     3721 gacatggaga ctttatcttt gtagttgcat ctatggcaaa cgttgctcaa ggtgctgcaa
     3781 catttgccat ttggttctta actaaaaatt ctaagacaaa aagtcttgct tcctctgcgg
     3841 gtgtctcagc acttcttgga attacagagc ctgccttgtt cggagttaac ttaaaatacc
     3901 gctttccatt cttttgtgct ttaattggtt caggaattgc tacagcagta gcaggattac
     3961 ttaaagtagt tgctgtttca cttggttcag caggtttctt gggatttctt tcaattaatg
     4021 caacatctat tccattttac gtattgtgtg aattgattag ctttgtagtt gcttttgcaa
     4081 ttacttactt ctacggtaaa acaaaagctg ctggtatctt taaagcagaa gaaattgctg
     4141 aacaaacacc agttaattct tcaaaagtag caacaacaga tccaatatct actgatgttg
     4201 aatcaacaca aacaaaagtt tcatcagaaa caattgttag tcccatttct ggtaaagtga
     4261 ttagtctttc ttcagtaaat gatccagtct tttcatcaga agctatggga aaagggattg
     4321 ccattaaacc aaatggaaac acggtccatt ctccagtaga tggtactatt caactcacgt
     4381 ttgaaactgg acatgcttat ggtattaaat caaataatgg tgctgaagtt ctaattcata
     4441 ttggtattga tactgtatca atgggaggaa aaggatttac acaaaaagtt actactaatc
     4501 aaaaagttag aaaaggcgat attcttggag catttgaccg ttcaatgatt gagaaagaag
     4561 gtcttgatga tacaacaatg attattgtga ccaatacttc ggattattca gaagtaaata
     4621 tagtagctga aaatacagtc actgagggta ctgatttact tgaaatcaaa taaatatatt
     4681 tccctgagta aagatggaat tttctttcca aaatcaaata atatagatta atcaaatatt
     4741 tgagaataac attttattta atcttttcat atatcaattg tatgaaaaat tattagaatt
     4801 ttaaaaaatg ataaaaattc aaaaagaaaa aatagtagtt atttacttgt catgttacta
     4861 aataaaaaaa taatttaaat atgatatata gtcatttatt tagtgatttc attctaaaat
     4921 aaagggagaa atgtctatgc tatacggtgc aattgaagca ggcggcacaa aatttgtgtg
     4981 tgccattggc gatgaagaaa tgacgatcaa ggaaagagtg agctttccga caacgacacc
     5041 tgaggaaaca atgcctttag tcattgactt cttcaaacaa taccaagcag atttagcagg
     5101 gattgggatt ggctcatttg ggcctatcga tatccatcgc gattccgcca cttatgggta
     5161 cattacctcg accccgaaa
//
LOCUS       DAEGIQ010000152         5156 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000152 DAEGIQ010000000
VERSION     DAEGIQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.158"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            31..810
                     /locus_tag="KMT45_002656"
     CDS             31..810
                     /locus_tag="KMT45_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006081.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            856..1800
                     /locus_tag="KMT45_002657"
     CDS             856..1800
                     /locus_tag="KMT45_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBE7006082.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1816..2595
                     /locus_tag="KMT45_002658"
     CDS             1816..2595
                     /locus_tag="KMT45_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBE7006083.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            2607..3305
                     /locus_tag="KMT45_002659"
     CDS             2607..3305
                     /locus_tag="KMT45_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBE7006084.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTSANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLSNAS
                     GLSTTLSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQVLAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3333..4097
                     /locus_tag="KMT45_002660"
     CDS             3333..4097
                     /locus_tag="KMT45_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBE7006085.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFRKDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKIAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            4161..4850
                     /locus_tag="KMT45_002661"
     CDS             4161..4850
                     /locus_tag="KMT45_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBE7006086.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKFIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSGRNDVSFHAMVDLDLEYISKSSLLYDLKIIFKT
                     ILVIVKPNGAY"
     gene            4853..>5156
                     /locus_tag="KMT45_002662"
     CDS             4853..>5156
                     /locus_tag="KMT45_002662"
                     /inference="COORDINATES: protein motif:HMM:NF015748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HBE7006087.1"
                     /translation="MKIRLFNIIIDNLNMSETVECTDKYIEKNTFTHLVGVNADKLVE
                     MGKNPELKRIVKESEIINADGASVVIASRLLGKGLKERIAGIDLMQELLELANKKKY"
BASE COUNT         1842 a          880 c         1093 g         1341 t
ORIGIN      
        1 tatctatcat caatacttag gagtgaatct atggaaaagc cattcgtcaa gtcattgact
       61 gctgtggcgg taggtgcagg tgctattgcg atctgtttgt ttggttacca tatcaataat
      121 cagcgacaac atcagcaacg gatcaattat gcagaatctg ccatcaccaa tcaaaaagac
      181 accttgactt ctttatcaaa agaagtagac aagctgtata gtacaaaaga aaaaattttt
      241 ctaaaccctg aaataacaga agaaacaatc acaagtttgt caaataagct atcctctgtc
      301 aaactttcag cagatgatta cgacatcaaa gaatcagaat tgcctaaaga agctgttgca
      361 atccaagatg agaagaaagc aatcgtgaca cagttagata gtgcagaatc aaaactgaag
      421 atccagacag ctgtcaacga tttatttact aaaaatgtat caaactggca gcaagctgta
      481 gacgatgtca tcatcaaaga aaaggtgaca agtgcagatg tcgctcgtgt acgtgaaaat
      541 atgagctttt ttaaagatag tccttggaaa acggttgtta tgcaatatct aggattcgct
      601 gatactcaga tcgcgcaagt cacgcaacta gatcagttat tcgatacgat gttgaaggat
      661 ggtcaagtga ctgccaccgc tacttacgat caatatttga cggtgctcag tcaaatagag
      721 cagatccgga atgaaaaaat aagcgcagca tacgcaacaa aagcacaaac tgttgctcag
      781 caaatgggct atagcaatat aagctattga cagttaaaac gttgattcaa gcaaggttag
      841 caaacggagg aaactatggc aagacgaaga agaaaatcaa gagggaaaaa aatatttctg
      901 attattttag cagttcttat cgtattagta ttagcaattg taggagtagg agcaaaatta
      961 tatctggatc tctcacattc tattgagaaa acttacgaat cagtggaacg aacccaagga
     1021 gaacgaacga aaccagttga tctggacaaa caagaatctt tctctgttct attgatgggc
     1081 attgatactg gagatttagg tcgggtcgat caaggaagat cagatacgat gatggttgcc
     1141 acagtcagtc ctgaagacaa acagacaacg atcgtcagca ttcctcgaga tacgtatgtc
     1201 gacatcgtag gtcgtgacac aaaagacaag atcaaccacg cttacgcatt cggcggagct
     1261 gccatggcga tggataccgt acaaaaatat gtagacatcc caatcgatca ctatgtctct
     1321 atcaatatga aagggctaaa agaacttgtt gatgcagttg gtggtatcga agtcaataat
     1381 gacctcactt tctcacaaga tggctatgac tttacaatcg gtaaaattac tttgaacggg
     1441 gatcaggcat tatcttattc tcgtatgcgt tatgaagatc caaacggtga ctatggtaga
     1501 caagagcgtc aaagaaagat cgtagaaggt gtagcgaaaa aagtattgag tctaaacggt
     1561 gtcagcaact atcaatccgt tttgaatgct ctagagtcga atatgaaaac agacatgagc
     1621 tttgatgaaa tgaaaaaaat tgctttagat tatcgggagg catttggtac gatcaagcaa
     1681 gatcagctgc aaggagaagg cttcatgcaa gataatatct cctatcaaaa agtcagtgat
     1741 caagaacttg atcgtgtgaa aaaagaatta aaagatcaaa tgaatctaga gaataaatag
     1801 gaaagtggag aaaaaatgga agagacaatc agcttgaaag aaatttttga aacattacgc
     1861 aaacacttgg taacaatcat catctcaatg tttgctggct tggccatctc aggaatcgtg
     1921 acgtttttca tcttgacacc aaaatacagt tcacaagcac aattgatcgt gacactgcca
     1981 caatcagaca ctgcaaatgt caatgatgtc aatacgaacc tgcaaatgat caatacgtat
     2041 aaagatatga tcgtcagtga tttagtacta aatgaagtca aagatcgttt agaaacagaa
     2101 gacaatgtga aaatgactgc tggacaaatc aaagatgcga tctccgtcaa tcagtcacaa
     2161 aattcacaaa tgttctcgat ccaggcaata agcacgaatg cagtgactgc tcaacagatc
     2221 gctaatacaa cttcacaagt tttccaagaa aacgccaaag acgtgatgaa tgtcgataaa
     2281 atctcgatta tttcaggtgc agttgccaat acgacacctg tttcgccaaa taataaattg
     2341 aatctagtga ttggcttagt gttaggaatg atggtaggcg taggtttggc attcttattc
     2401 gaattacttg atcggacagt gaaagatgaa aaatatgtga cggatacatt aggattccca
     2461 atcttaggaa cagttccaga aatgtcagcg aaagaattga acgctggtat ccgtaaaaaa
     2521 ggaacagctc ctgcacgacc aaaagctaca aacgataaaa cagccccatc acgcagaagc
     2581 cgctcaagag tctaggagga agaaaaatgg cacgaacaca gaaacaaaaa gcagcacaaa
     2641 ctcatgcagt tagtttgatc acattaacaa acccatcttc accaatcgcc gaacaatatc
     2701 gtacgatccg gacgaatatc cagtttgcct ccgcagctgg tcagcaaatc aaaacgattg
     2761 tcgtgacttc atcaggtcct ggagaaggga aatccaccac ttcagcaaac attgcagtcg
     2821 tttttgctaa atccggtcaa cgggttctac tagtcgatgc agatttacgt aaacccgtcg
     2881 tctataaaac attccagttg agtaatgcca gtggattaag tacaacactt agttcatcag
     2941 gaagtgtagc agatgtaatc caacgaacgc cagtcgagaa cttgtcgatt ttgcctagtg
     3001 ggcctaagcc acccaaccca tcagaattgt taagctcgcc aagaatggat caggttctag
     3061 ccgaagcaag acagttattt gacgtggtga tattcgatat gccaccagta gtagccgtaa
     3121 cagatgcaca gattatgtct tcaaaaacgg atggcacctt attggtcgta cgtgaaaata
     3181 cgtcacgtaa agaatccttg actaaagcaa aagaactgct ggagatggta caagcacgag
     3241 ttttaggtgt tgtatacaac ggagcagaac atagcaaaga tgctggttat tactacggaa
     3301 attaaggaat aagaagagag gagacaagag aaatgatcga tctacattgc catatcctac
     3361 caggaatcga tgacggggca gaaaacttag aagcaagtat tgcaatggct gaaaaagcga
     3421 ttcagcaagg cattacgcat attttatgca caccacacca caataatgga aaatacagca
     3481 atgaaaaatc acaagtcatt tctctagtcg cttcgttaca agccgaatta gagaaaagac
     3541 agttgccttt gacgttattg gaaggacaag aagtacgtat taccggaacg ctcatcgaag
     3601 acatccaccg agatgaaatt ttatttacag atttagatga tacatatctt ctgatcgaat
     3661 ttccgacact ggaagtgcca ttatatgcag aacggctttt tttcgagttg ttagaactag
     3721 gaaagacacc agtgatcgtc catccagaac gtaacgcgca ttttcgtaaa gacccgaatc
     3781 atttgcttcc attcctagat atgggatgtt tggcccaact tactgcgcca agttatgttg
     3841 gtgtatttgg caaagatatc cagaaaatag ccaaaacgat ggtcaaacat aatcttgtgc
     3901 agatggctgc ttctgatgcc catggggtct ctaagcgcac cttttactta aaagagtgct
     3961 atgagcagat tgccaaggac tttggtaagg aaaaagtagt acagatgaaa caagtcgcaa
     4021 aggatctgat caacggggat gaaatcagtt atccaatcta tgaagaggta aaaaagaaga
     4081 agtttggttt attttagttc tttattcgta gataaaaagg aaatgaggag agagtgaaaa
     4141 tagtgaaagg gactgaaagt atggaaagtg aagatttatt atctcgtatt agtgaagtac
     4201 atcctaatca agtttggttg gataaagaaa aagttgaaaa acgatatatc tatttatttt
     4261 ttaaaagaat gattgatatt gtaggaagca ttattggttt gattttagct tctcctttga
     4321 tgttctatgt agcttatcga atcagaaaag aagagccagg aagtccgata ttttttagcc
     4381 atacacgtgt tggtaaaaat ggaaaaacat tcaaaatgca taaattccgt tcgatgtgtt
     4441 tagatgctga agaaaaattg gaagaattac tggatcaaaa tgaaatagaa ggcgcaatgt
     4501 tcaagatgaa agaagatcca cgtgttacca aaataggtaa atttatacgt gctaaaagta
     4561 ttgatgaatt gccacagcta tggaatgttt taaaaggaga tatgagtcta attggcccac
     4621 gtccaccttt ggaaagggaa gtagctgagt acacgagata tgatcttcaa cgcttgtatg
     4681 taaaacctgg atgttcaggt ttgtggcaag ttagtggtag aaatgatgtt agttttcatg
     4741 caatggttga tttagattta gaatatataa gtaaaagtag tttattatat gatttaaaaa
     4801 taatttttaa aacaatttta gttattgtta agccgaacgg agcgtattaa taatgaaaat
     4861 tagattattc aatattatta ttgacaatct gaatatgtca gaaacagtag aatgtactga
     4921 taagtatatt gaaaaaaata catttacaca tttagttggg gtcaatgcag ataagttggt
     4981 tgagatggga aaaaaccctg aattgaaaag gatagtaaaa gaatcagaaa taattaatgc
     5041 cgacggagct tcagttgtta tagcaagtag attattgggt aaaggcttga aagaaagaat
     5101 tgcaggaatt gatttaatgc aagaattatt agagttagct aataaaaaaa aatact
//
LOCUS       DAEGIQ010000153         5155 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000153 DAEGIQ010000000
VERSION     DAEGIQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.159"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(82..489)
                     /locus_tag="KMT45_002663"
     CDS             complement(82..489)
                     /locus_tag="KMT45_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006088.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(508..1326)
                     /locus_tag="KMT45_002664"
     CDS             complement(508..1326)
                     /locus_tag="KMT45_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBE7006089.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(1552..1845)
                     /locus_tag="KMT45_002665"
     CDS             complement(1552..1845)
                     /locus_tag="KMT45_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBE7006090.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFNSNQQRKVKKQID"
     gene            complement(1857..2036)
                     /locus_tag="KMT45_002666"
     CDS             complement(1857..2036)
                     /locus_tag="KMT45_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006091.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQLISMNPFLFLQIGVDTRI
                     MRVLNYYCKRNNKRK"
     gene            complement(2033..2383)
                     /locus_tag="KMT45_002667"
     CDS             complement(2033..2383)
                     /locus_tag="KMT45_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006092.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(2376..3701)
                     /locus_tag="KMT45_002668"
     CDS             complement(2376..3701)
                     /locus_tag="KMT45_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBE7006093.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            complement(3870..4454)
                     /locus_tag="KMT45_002669"
     CDS             complement(3870..4454)
                     /locus_tag="KMT45_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7006094.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(4665..4868)
                     /locus_tag="KMT45_002670"
     CDS             complement(4665..4868)
                     /locus_tag="KMT45_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006095.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(4878..>5155)
                     /locus_tag="KMT45_002671"
     CDS             complement(4878..>5155)
                     /locus_tag="KMT45_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006096.1"
                     /translation="GIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVG
                     WPSRPPQSRNWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
BASE COUNT         1524 a          960 c          757 g         1914 t
ORIGIN      
        1 atatttaatg tataacttat actttaatat tcatttatct agtataactt atactttaat
       61 tgaataccgc atgaaaaaaa attattttgg taatttttca aactcttctt gtatttcttc
      121 tcttatgtgt tcttcttttg ctactcctag tctatgctgg atatatgagt ctgctaatgt
      181 attaacaatt ttttcgaaag aaattctccc tttttcactt tgtaactccg tcatatagtc
      241 ttgtaaaatt gagagtttgc tatttaatgc cggagagatc tttttaggaa ctgatacctt
      301 gtttctttta tcaggaattg aaactggtcg gcctcttttt ttcaatgtaa tatttcttgt
      361 gttactcaat gcactattgc taaagttata ttcaccgctt attttcacat cttggcgttt
      421 gttattgggt aataatggct caaattcacc attctccatc attttaggaa tatcaaaatc
      481 tttaggcatt acttatgcaa ctccttatta tttatttaaa aactcttgtc tagcatctag
      541 ttctgccaaa atatttataa atacttggtg tgctttataa tcccattgat caaaatttcc
      601 atttttgtat tttcttaaat gtataccaat tttagaatac cttctcaatc ttttttgata
      661 atgaatgaga tttttggata ccacggttcc gtatttttca attgcttctt ctaggatttc
      721 ttcatcaaaa tcatcatttg gttctaccat acaaggtaca atatctataa tttgcaaatc
      781 aagatcgtat ctatcgacca tgaatttttg ataatttaca tatttgttga ctccctctaa
      841 ggattcttca gctgtttgaa atgaaattat cgaatagtct gaagctgcca tagcattatc
      901 ggaaaattcg ctaatggttg gtggtacatc aataaatatt tcttgataat tgttctttat
      961 tggaagaagt aattttttca agacagtaac ttggtctaat tcattttcat gtttattagc
     1021 aaaagcggag aatgatcgaa aactagtgtc acatgccaga aggtcaagat tttcatttac
     1081 cttaataata ctacttgcta attctccatt tgcgacacca tttgttatag tggttttgat
     1141 atcggttata ccgaaagttc ttgctatgac ttgtgttaag ttcgcttgag ggtctaaatc
     1201 aataataagc gtttttctat ttcttatcat tgcattgtca taagcaagca tgctgactat
     1261 cttagtttta cctactcctc ctttaaaatt gccaactgta aatgtagtcc cttgaaaagt
     1321 atccatgtaa atcctcccaa tctttaattg ataattaaag tataacttat atatcggaaa
     1381 tggtcaacat taaaagtata acttaaataa taatatctaa aaacgtttgg atatagcggt
     1441 ttttaataat aaaagtataa gttataccat atatatttat ggtataactt atacttttat
     1501 tattaaaggt ataacttata ccttaaatat tgcttttcga aaatagctat tctagtctat
     1561 ctgtttcttt acttttcgct gctgatttga attgaattga tcatattcat tttctagtat
     1621 taataacttt tcgtatacat cggtcatatt catactagaa gcaagactta gatcatatac
     1681 aaatgaagca ggtaatgatt ttattctacg ttgtcgggtt atccagcttg caaccgttcc
     1741 ttgtgggtag gcatataact cacaaaattg ttctacagtg attcctaaat tcttgataat
     1801 gtaaacattg atcgggtgcg agtaaacaaa tgtattgata gacatggtgc gtaagcctac
     1861 tttcttttat tattgcgttt gcaataatag tttaacacac gcattattct agtatctact
     1921 cctatttgta aaaacaaaaa aggattcata gagataagtt ggccataacc ccaaatctct
     1981 tctgatagct gatcaaaggt tatatacttt agctcatatt gctggataat gttcattcta
     2041 atcgactcca tttattatcc tggtaaaatt cgatatgacg tatttcgtcg tagtgaattt
     2101 tgtcttttcc aacgtagatc cccagctcat cactacctaa gatcttgcca acaatatctt
     2161 ctggatagta tccttcagcg tttcgttctt ctttttgaat agatactgat ctatttttta
     2221 tgactgcagc gttcaatact tccgcaatct cttcagtcgt catttgttct tttgctaaat
     2281 tttgactggt tcgttgttca ttgtcggcat caatttcttc agtatgatca gataaataaa
     2341 accctgccca ctttaaacgt ccacgatcat tgtaatcaat catttttaat tcccccattt
     2401 ccgcccgcat gaccaccaac aagtgtgctt ctagtaatgc ttctagcgct ttctaacatt
     2461 gaagaagcat gtacaatcga cgtaaaacca tatttttgcc gaatcttgtc aataatttta
     2521 tctaaatttt cttcgttaat ttgcttttgt ggatcactaa ataaatccag ctgaagcgaa
     2581 tcggtgtaca caagcttgct ataggttatt ccaacgtgcc ttacttcttg tccttcataa
     2641 tacttatcaa acaagaaaag acaataattc tgcaattcct ttgtattatc tgtgataggt
     2701 attttcatct ggtgtgaaaa tcctggtctc gtttctaaga tcgacgtacc aatgtttaaa
     2761 tgaacacatc ctgttttaca attatgctgc ctgatacgaa tggctacttg ttccgccatt
     2821 tcttttacca ccagctcgat ttcatttctt ctagtataat ctcgtggtaa cacttgtgaa
     2881 ttaccataag atttttcctt tgtcggttct ggcgggattg ctatatctgt tcgatcgatt
     2941 ccgttagcat ggaagtacag ctgtaatcca atcacaccta atctattctt aatcgtataa
     3001 ggattccagt tagcaaggtc tctaatgctc aaaattccca tttggttgag cctttttttc
     3061 attcgagaac caattcccca aaagtccgtc atttcgggaa tattccatac tttctcagga
     3121 acgttttcgt acgtccattc cgcaataaat ccttcattat gctttgcttc attgtctaag
     3181 gctaatttag ccaaaagggg attatcgccg acgcctaccg tagcaatcaa acctaactct
     3241 tctttaatac gttcttggat catttgagcg agcttcttcc ttcgctgact tcgtgtttct
     3301 tcagtcgtaa aaagattcag tgattttgtc acttttaaaa tcgattcatc gatcgagtaa
     3361 atcagtagat cttcatcagc cacatatctt ctgaaaatat tatttacctg catatttcgc
     3421 ttgatatata ggttcatacg tggtggaaca acgtgtagtg ttttaggaaa tggttgtggt
     3481 aagtcacgtg gtctactcac atttgtaata ccataccgct tttttgcttc aggagaagaa
     3541 gctaatatca atcctgaacc agtattatca gatcgactca taacaacaag ttctgttgtt
     3601 aatggatcta aatttctttc tatacattcg acacttgcgt aaaagctttt catatcgatt
     3661 aaaaagtaat catttactgg ctcttttgaa taatccatca taaataacac tccattcttc
     3721 cacattatac aaacatatgt tcgatgttgt aaagaagaaa tagctaaata tttgagaata
     3781 agttatcagt agacacttac aaaaaattct catactattg tcacttttct agataaatgg
     3841 caaaaatttt cagcattaaa tgccaaaaac taatgagaat ttgtctttcc atttttctcc
     3901 tccatttttg ccaaataacg ataaatggtg gttcttgaga cgttccaccg ttccgcaata
     3961 gtcttaatag gtgtaccgct atccactaaa gtcttcagta attccaaatc ttgttcagac
     4021 aatttttcag gacgaccacc aaaccgtcct cttgcacgag cagctgctcg tcctgctgcg
     4081 gatcgttcca ggatcaaatt acgctcaaat tctgcaaatg ccgcaaacaa atgaaacatg
     4141 agctgtcctg tagaacttga tttgtccatc gtaatatttt cttgaatact atgaaaactc
     4201 acaccacgct cattcagtcg attgacgatt gaaatcaaat cttccatatt tctacctaaa
     4261 cgatctaagc gccaaacgac aagtgtatcg ccagagcgaa caaaatcaat ggccatttct
     4321 aatcctggtc gattactttt tgccccactc atatgatcag taaaaatttt ttcacaacca
     4381 aatttattta ggctgtcttc ttgtaaatcc aaattttgta aaccagtaga tacccgggcg
     4441 tatcctattt tcaaaaaaat cactccttca ttaattttat tgtaacataa ctttatccat
     4501 atagtttaac tgaacataga tattgttatg ggtttatgaa catgaaatga attgtttttt
     4561 tgaaaaataa agcaaaaaaa gaaagtgtcc agaaatgggt gattttatga acactttcaa
     4621 ttagaaaaaa agaatgtcga ctagacactc tttttttaaa atcattattt ttctttttga
     4681 tatgtttttt ggtctctttt ttttcttttt tcttcagaat cttctagatt agaaattgat
     4741 ttttgaattt tttgtgtctc ctgtaaagtt cgctgaactt gtaaagctga ttcttctttc
     4801 ggtaaattaa ttttacttag aatatcgcta tcttgataaa aatttggtgt gttcacaaat
     4861 tctggcataa aaaatcccta ctttctagtt aaggttcctg aataagtcgc aatccaattg
     4921 tttccagctg atttataact ttgtaaatac aattttccat agtaccaata accatttctt
     4981 ttttctttcc aattccgtga ttgaggaggt ctagaaggcc aaccaacttt atcaaaaatc
     5041 atcgttcctg tcactcgtat cgttttagaa gctgctcgag caagtaatgg agaaactatt
     5101 aaataatttt cagtaacagt taagtcgtta gttgggttac caatggctat tccgt
//
LOCUS       DAEGIQ010000154         5120 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000154 DAEGIQ010000000
VERSION     DAEGIQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.160"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1150..1815)
                     /locus_tag="KMT45_002672"
     CDS             complement(1150..1815)
                     /locus_tag="KMT45_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7006097.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1995..3977
                     /locus_tag="KMT45_002673"
     CDS             1995..3977
                     /locus_tag="KMT45_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBE7006098.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3981..4619
                     /locus_tag="KMT45_002674"
     CDS             3981..4619
                     /locus_tag="KMT45_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7006099.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4647..5075
                     /locus_tag="KMT45_002675"
     CDS             4647..5075
                     /locus_tag="KMT45_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006100.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1697 a          781 c          940 g         1702 t
ORIGIN      
        1 aaatggtaag tttttccttg tttgttttag tttaaactag tgtgtaattc aaaaatctat
       61 atggaagggt ctgtggggta atacttaaat aaaggctagg aacctgataa catcaaggtt
      121 cctagccttt ttataataaa atgaagcgaa aataatttct tttaaagagc cgagattatc
      181 agctatataa gagtgaattt gatgcaaaaa acagagccga aacagtcttg gtgttcgtag
      241 ctctgcttat atgtttcgaa tttaagacaa agagttggca aaagatgttg ataaggaaga
      301 taagataact gtgttatata aactaggtaa ctaactgtaa caagctaatt ttaagagatt
      361 cttattagaa gacgtttagg aatataacta agaaaatgat tatacaggta attgtcattt
      421 caataatgtt ttctaatttt cgcgtctttg ttttaagaaa aaggccagaa tcagttggaa
      481 aaaagttcgt acgatagtga agaggggcaa aatttcctac taatattccc actaacaaga
      541 gataaaacgg ccaggttggt gtgattcctc tataaaaaaa tgcccatgct accgaaatcc
      601 catagattaa caactgaaca atttttacaa tgaaaaaatt gttctgccat tactcaaagt
      661 ctcttcctcc ttgtatcgtt tatttgtttc attttatcat tatgataggc gcttgaacaa
      721 tccattttgt attttattga gtactcttgg ataaactacc agcgattatc atatttattt
      781 atgttactgt aagccatcat ataaaagtaa atgagcagat catagaaata taaaaaagaa
      841 ctaaaattga tctaaattat gtgcagcttg actacgtacc aatctatatt acttactaga
      901 ttggtcgtta atctattcgg atcgaatgag caaaatattt ttgtgaatgt ccatccttga
      961 ttatgattcc aaaaagaatc attgggaggc tgtggcgtaa atattatact ataagccaga
     1021 aatacctatc attctgaact tttttattta ggaactttca tctcaaggct ttttcaatta
     1081 atttgtaccc ctgttttttg gataagatca tgtgcattga ttttttctat ctctacgttc
     1141 atctcctact taatgcctat ctggctcctg agggcttctt tttaaggcgt cgtcatagtc
     1201 ttggctgagg atttcttggt aatctgaggg aatggcttcc caagtgagca ggcgaacgtt
     1261 catgacgtgt tttcctttgt gtttgattac agcatggttt ttctcttttt ctacctctcc
     1321 agcgtgaaaa gacagccatg taccatggcc aaagatatca ttacataccc aacgttcgat
     1381 ggaattgtcc acgtatccta cagggagtgt ggtatagccg atttgtggtt tcaaaaaagg
     1441 attgaatcga tcgatcgttt catttttgta ttgataattg ataagaaaaa gatagaccaa
     1501 acaaaagacg agcagaaaga aagatagtgt ctttttgagt gagaagggtt gttttgatga
     1561 ataagatttc ttgttaacaa gaggttcatc tggcaatgtt ggttctattg attcggtagc
     1621 tgattcaata gaaggaaaat caatcggcag acctaaagta tgatgtaaaa gtgacagcat
     1681 ttcaaagtct ggcattgtac gagaattttc ccaactagaa atcgttttat cggaaacacc
     1741 aattttttcg cctaattctt tttgcgtaag cttacaattt ctgcggtaag ttcgtatttt
     1801 ttctccggtg ttcatcttct tctcctttcg attctacagt atatagattt attctacatg
     1861 acctatacac ttgatagata cgtttttttg atgaaaaacg gtatgatttt atcaaagaat
     1921 catttaagta agggagagtg gtgataatca aaaaaaagtt cacagtttta ctcacaatca
     1981 tagtgaccgg tatcatgagt ggttttgcct tggttagtat aaataaacag gtactctatc
     2041 ggacaaatga atttttccat caaacagata cttatactcc gattcgattg ccacaacatt
     2101 ttattgggca agaagaatat gagcagctaa tcatggtatt tgaaaatgtc tcatcagaaa
     2161 ctgagctttc ttatatgaaa aaaacagtaa atgaatccaa tgaagtagat acatcttgga
     2221 acttcaagat acagccaaag cttgaaatga cattgtatta tcaaggggat gtcaaatctt
     2281 ctcacgagaa ggtaccctat tctaatacct cctttcgcgt aacgtccatt gaagaatcga
     2341 tcaaaataaa agattttgaa ggtgaatttt tcttgatgac gagagaaaaa gaagcctatg
     2401 aacgggtcat ttcactattc actgatcgat tcaatcaagc atttcacact gattatgcca
     2461 ctaatatttt tgaagacttt gaaaatagct attcgtattc ggttatttca aatacagacg
     2521 tatataatta tcgagtgtat ttaactattg gattgattct tttattcctt ttgttatcat
     2581 cctttttctt attgatgaat aaagaaatca gtttattgtt gaataatgac ttttcattac
     2641 atcaagtatt gtggtgttta ttggggaaaa agaaaatcat tattacattc ataattgcag
     2701 gaatcattgg tggttttatt tttatgtatt ataaggagca atttctttat ttgttccaga
     2761 tgtacgtttt cttgttcagc ggaatatatg gtatctcttt gatttctctt ttattagttg
     2821 aaaaaatcgc ttcgttaaga gaagcgttca actatatcca atacattctt tttacatttg
     2881 gattgttgtt ttctcttctt ttgaccgttg ccaatgtcga attagcaact atcgtttttt
     2941 cttcttcaca gttagctgtt tcccatgaac caccaaaaga aatcgctggt gaggactatc
     3001 atgtatttta tccagtaacc atcgggaaaa accacgtgga ttttatttat tccaatcgtt
     3061 ttgcatcagc agcagatcag gaattatatg aaactttgaa taaaaacgga agtatattag
     3121 tcaatgtatc agattacttg aatgaagaaa atgaacagtt tggcagaggg ataaaaataa
     3181 atctgaatta tcttaagaaa tttcctttga ttgatgtctc tggaaggttg atccaaataa
     3241 ccgaaaaaga aacgcaccct gttatattga tccctgaacg atatagacat acagatttaa
     3301 aaaatgacct ggcgcaaata acagaatatt atcaagaaat cagtgaaaaa gaaccgaaat
     3361 ttctatttat aaaaaacaat caacccattt atacatttat tccttctatc ccctggatcg
     3421 aacattatcc ggttgttgaa atactgacat tgaaaaacag tatgtatttg gaaagaaata
     3481 ttctcagcgg agaaatctat ccaccgttaa agatcaagat aggctctttg tcgaaagaaa
     3541 ggttatttga gctgattgaa gaaagtcagt tacgtgataa cttgcagtta tcatttccgt
     3601 atactcagac tgaagagata gctgtcaaag tgttaagcag atcatttcat tacttgatac
     3661 agatattttt actcactttc ttttctttct tcttgttgag ctatgtcatg ttatgcatat
     3721 attttgtcta caattgtcgg aaaatcgcta tctttcgttc aagtggttat tcgctctttg
     3781 aaacgtataa agatttcttc atgatgaacc tcatcaaatg gggaacgaca agtgtaatct
     3841 tcctttttct tatagaaaga gagcctaaat atctttttaa tatttttttc tttagcttca
     3901 ttgattttat tctttcagtt gtttttattt tgtctacaga aaagaaaagt cagttattgc
     3961 tgatgaatgg aggttaacaa ttggagccgt tgatagtgat aaaaggtgcg aaaaaaatca
     4021 ttcgaaacaa aacgattctt catttggatc gtttcactgc ggatgctggg gatcttgtga
     4081 ctgttgttgg agaaagtgga gcaggtaaaa gtacgttatt gaatatatta ggattgaatg
     4141 atcagttcag ttcaggacaa ttctatttgt tcgatcaaga gacgaaacaa ttaactggga
     4201 gagagaagcg cttgataaaa agaaatacca tatcttttct ttttcaagat tttgggttga
     4261 ttgaatatga aacaatcgat tttaatttag atattggatt gaagttttcg aatttaaatg
     4321 tttcagataa gcgaacagca aagtatgagg cattgaaaaa agtaaatata aacaagaagc
     4381 taagtacgcc tatctacaca ttatctggtg gtgagaagca aagaatcgcc tgtgcgagag
     4441 tcttattgaa gaatagcaaa atcattttag ccgatgaacc gactgggtca ttggataaaa
     4501 gaaataagga taaagtagtt gaaatcttat cgcaacaagc ttcccaaggt acgttagtta
     4561 tcgtagtcac gcatgatcca tatctgacca aaataggaaa caagaagatc atgctttaaa
     4621 aatcaaaaag gaaaggatgt cgttttatga aaaaagcagt tattatttgt gcactaggga
     4681 taggttttgt tggaggaaac gctatgtcat ctgaagtgga agcaaattca gttcacggtg
     4741 aaatctatta caaatcttta aagccaggag agattcattt tgatgctaaa ggtaatatta
     4801 ttaagtcaaa tttaccagta gaccgggaaa tttctagaac agcaattaat catagctctg
     4861 gaagatgggt ttattatagt gatacaactt gtacgagaaa aacaggtcat tctaaccact
     4921 atagtcgtgt ctataatcgt cacggtgcaa aagcgaaagt tggaaactct attcgatcag
     4981 caaatgcaac aaaaggagta tgggcctatg caagtgcgac aggaaataga actgacacat
     5041 tctcatgttg gtacaatcca accggttggt attaagtatc caaaagttta tataaaatag
     5101 aaaaaacata taatgaaaca
//
LOCUS       DAEGIQ010000155         5012 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000155 DAEGIQ010000000
VERSION     DAEGIQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.161"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="KMT45_002676"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="KMT45_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBE7006101.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEIAVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="KMT45_002677"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="KMT45_002677"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBE7006102.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="KMT45_002678"
     CDS             complement(2169..2906)
                     /locus_tag="KMT45_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBE7006103.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(3003..3524)
                     /locus_tag="KMT45_002679"
     CDS             complement(3003..3524)
                     /locus_tag="KMT45_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006104.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDV"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="KMT45_002680"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="KMT45_002680"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBE7006105.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="KMT45_002681"
     CDS             complement(4401..4886)
                     /locus_tag="KMT45_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBE7006106.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1334 a         1120 c          857 g         1701 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagcaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc aacaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 cttcaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag ta
//
LOCUS       DAEGIQ010000156         5003 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000156 DAEGIQ010000000
VERSION     DAEGIQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.162"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            74..1215
                     /locus_tag="KMT45_002682"
                     /pseudo
     CDS             74..1215
                     /locus_tag="KMT45_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1341..2420)
                     /locus_tag="KMT45_002683"
     CDS             complement(1341..2420)
                     /locus_tag="KMT45_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE7006107.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2529..2969)
                     /locus_tag="KMT45_002684"
     CDS             complement(2529..2969)
                     /locus_tag="KMT45_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE7006108.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3026..3436)
                     /locus_tag="KMT45_002685"
     CDS             complement(3026..3436)
                     /locus_tag="KMT45_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBE7006109.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3617..4051)
                     /locus_tag="KMT45_002686"
     CDS             complement(3617..4051)
                     /locus_tag="KMT45_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE7006110.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4151..4999)
                     /locus_tag="KMT45_002687"
     CDS             complement(4151..4999)
                     /locus_tag="KMT45_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBE7006111.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1830 a          962 c          844 g         1367 t
ORIGIN      
        1 aacagtaaac atataggtag aatctctact atcttatttc ttgcatacaa aaaaatcaag
       61 gagtgagaga gttatgagat attcaaccat cttaatatca acatttgcag ccttaagctt
      121 atcgcttact ataaatcaga cagtccacac agaggagaca gaaacagcca aatggacaga
      181 aaccaagtgg gccaacgtca atcgaattac ctttcaggat gaattttcat ccttcaattc
      241 aggagtcgca aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa ttgactattc
      301 gggagactta gcacctatac tacaatcagc tgttacattt gagaatgttg atcccacttt
      361 gtcgattggc tatgacgaga acactttgac tatccaacac aagcaagcga tatttacctt
      421 caccgttaca ttaaatcagg acttgttaaa accagcgctt tttactgtca aggtatcaga
      481 tggactaccg aacgatcatc atttaacgcc gtatagtcag cggcagacaa ttgagcaaga
      541 ttcagccatc gactcaggag gagatcttgt tgaagaacca acagataaac cagaaaatga
      601 aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac cggaaataga
      661 acctggagaa gaacccgata cagaaactca accagagcca gacaataaat caaaacctga
      721 aataacccct ggagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca
      781 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga
      841 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
      901 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
      961 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
     1021 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt
     1081 cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa agttatactg
     1141 tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag atttaaacaa
     1201 aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat
     1261 atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc gttcatactt
     1321 tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg gttcgggatc
     1381 tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac aatcaagccc
     1441 caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa aatcggcagt
     1501 tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag atggacaatc
     1561 aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata agctatgaaa
     1621 gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa aatgaaaatc
     1681 atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc
     1741 aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa tgtattgaca
     1801 aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact ttttaaaaac
     1861 aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact taagatgaat
     1921 gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag cgcaatcgaa
     1981 gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg gatatattgt
     2041 aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat caataacgga ctggatcata
     2101 tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata gataaggtaa
     2161 acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac aaatcgctga
     2221 atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa aatggttgta
     2281 atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt gagacggcgt
     2341 attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc caaataacaa
     2401 aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag aaagagcaaa
     2461 aagaaaaacc gctaaatgac gttatcttat gagttggttt tcctcataaa atcgttcatc
     2521 tagcagtttc agcccttttt ctggtcttga tgaaaattat ctagcatttt tcgggtcaga
     2581 tgttgcagtg tatccaattc ttcctgttca agtggaagat aagacttgat cttttgtgga
     2641 ataccttctg cttgtttttg caacgcagca ccttgaggag tcagcgtaac atttacgatt
     2701 ctttcatcac ccgtttcacg ctgacgatta atataatttt tttctgctaa cttttttagt
     2761 aagggagtca gcgtaccaga atccagacac aaatcatttc ctagctcttt cactgaagtc
     2821 tttttctttt cccataaaac taacatcaca agatactggg tataggtaag attcagcggc
     2881 ttgaggaggg aagtgtagtg tctaatgatt tccttagagg cggcatataa aggaaaacat
     2941 acttgattaa ctaaatatag atcttccatt tttccacctc cgctgtttta ctatagtgta
     3001 tcatagcgga tttacacaaa aaagtctatt cactaactgc tttcagagta acttctatat
     3061 ttccagctgt agcttttgaa tacggacaca ctttatgcgc tgcctctaat aattcttgcg
     3121 tttcttccag agaaagacct tcgatagatc cttcaatctc tacacctaag acaaagctcg
     3181 tttcactttc tgaataaagg gaaacttgtg cactgacagt agaagcccca gagatatttt
     3241 tttgctgttt gatcaattct aatgcactgt taaaacatgc actgaaacca gctgcaaaca
     3301 attgttcagg gttggttgca ttttcgacgc gtttaccagg agctgcaata ttcaattcga
     3361 atgtacgatc gggattatga acttctccag agcgtccgcc tgtgttgatc attttcgtag
     3421 aatacatttt tttcataaaa ttaccacctt taattatatt ttatacaatt gaattttaca
     3481 caatataatt gtggaaagca aagaaatgga ttggcataag ataaataaga gaaagcacat
     3541 gaaacagacg cgctttctcg aatagcaaac atatacaaaa aaatatcaga gacaaaaact
     3601 aatctcatca taaacattac atatatttct caaaaagttg ttttctttct tcaaaactta
     3661 cttcacgatg gacctgatga agcatccata aataaccaaa aggatctctg aacattgagt
     3721 tagtcacacc aaagtccatc atttctgtca tttcttgaat ctctacacag ccatttcgta
     3781 atgctttatt atgtgtgtcc tcgatatcag agacgatgat attgaaccag atcgtatttg
     3841 gatgttcagc agtaggagca gtcaaaccaa gagacgtgtt ttcatccagc atatggaatc
     3901 tcgtgccata caaggtaaaa atgacttcat tattgcctct agggtaatct gttgcctcaa
     3961 tcagttgtat ttcatcaaag attgtttgat acagttctag tgctttcaaa ctatcattta
     4021 atacaaaatc tatttccgtt cctttatgca taaagatctc ctctcctttt cagtcgaaca
     4081 gttactagat tcttttatta taccagttaa agaaaacgca ttcaaaaaaa tagcggattt
     4141 acttaccaac tcattcgatt ggatgataag taaatccgcg tcccattact tctgctacgc
     4201 tgctgacaat gacaaaggca cttggatcga tttcttctat caatggtttc attcgggaaa
     4261 attcatgatc agtcacaacg atcaataaaa cctcttttga ctttcgatta tacccgcctt
     4321 ttgcctctag cagggtaagg ccacgtccaa tctcagcact taagcgaagg cggatgtctt
     4381 ccaaataatg ttcactcatg accataacag atttctttcg attgatacct gtttcgatat
     4441 aagtcatgac aattgaagtg attgcaatag ataaaatagc ataaagaaac tcttctattc
     4501 caaaaacaaa cagattaaga gaaacaacga atgcgtctgt aaaaaataag ccaatcgaag
     4561 tattcaagtg gaaatatttt ttaaaaatga ggggagggat cgtcgtaccg ccgctagaag
     4621 agttgttttt atataaaatc gcaactccta aagcaaaaat cgcacttcca aaaatgacag
     4681 acaacaaccg atctgaagta atcattgttt ctggaactat tgcaatgatt aaaggaaaaa
     4741 acaagcttcc gaaaagtact ttaaaaaagg gttttttacc taaaaataga agagctagta
     4801 ccaacataac gatattcaac actaaaataa ctatagctcg gtcaaatcct aaaacagctt
     4861 ccagtaatat tcctagaccg ctgacacctc cagcagcaat atgatgaggc cctaaaaaca
     4921 tattgatagc gactcctaat atgaagaggg caaaaataat cttaatgtat gtccatataa
     4981 attgtctcgt ttctttcatt tat
//
LOCUS       DAEGIQ010000157         4848 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000157 DAEGIQ010000000
VERSION     DAEGIQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.163"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1390
                     /locus_tag="KMT45_002688"
     CDS             <1..1390
                     /locus_tag="KMT45_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE7006112.1"
                     /translation="MEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKG
                     FRNTYFKQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEI
                     EAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKP
                     RFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLK
                     YYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELN
                     KLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAH
                     IFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLP
                     VVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVK
                     ERLEQIIGSLTG"
     gene            1397..1774
                     /locus_tag="KMT45_002689"
     CDS             1397..1774
                     /locus_tag="KMT45_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006113.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1925..2707
                     /locus_tag="KMT45_002690"
     CDS             1925..2707
                     /locus_tag="KMT45_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HBE7006114.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2833..3564)
                     /gene="erm(A)"
                     /locus_tag="KMT45_002691"
     CDS             complement(2833..3564)
                     /gene="erm(A)"
                     /locus_tag="KMT45_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HBE7006115.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVIAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(3622..3681)
                     /locus_tag="KMT45_002692"
     CDS             complement(3622..3681)
                     /locus_tag="KMT45_002692"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HBE7006116.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            4073..4735
                     /locus_tag="KMT45_002693"
     CDS             4073..4735
                     /locus_tag="KMT45_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBE7006117.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         1705 a          693 c          926 g         1524 t
ORIGIN      
        1 tatggagttt tatgagaact tttattttga tggagaggag tgggataaag acgtttggga
       61 tagacgtaac ttacctttgc cagatgataa ggttaaccca acacaatatg aatatacaat
      121 taactttaaa gggtttcgga atacatattt taaacaactt gtaaaaagat attgtaagtt
      181 gagattgaac gtggatagct tttcctatgt aagtgatatt gcccaaagac ttaaagagtt
      241 ctttaatttt ctggacatga aatttaaaca agttcagaga gtacaccaat taacgagagt
      301 ggaaattgaa gcatatttaa gtgaactaaa catgatggga ataaaaccta gtacaataac
      361 tgggaggatc tctatattgg aaggactatt tagtaccctt cttaggctag aatgggatga
      421 tgttccttcc aaaatattaa tttattctga ggactatccg aaaataccaa gagcaaaacc
      481 acgctttata gatgaattcg tcctagagca attgaacagt catcttgata aattacccga
      541 atatatagct acgatgacta tgattgttca agaatgtgga atgaggataa gtgaattgtg
      601 caccttgaaa aaaggctgtc tattagagga caaagatgga gatttctttt taaagtatta
      661 tcaatggaaa atgaaaaagg agcatatagt tccaatatct aaagaggtag ctttacttat
      721 taaagttcgg gaagataaag tttcagagga atttccagat agtgaatacc tctttccaag
      781 aaaagatgga tcgccattaa aacaagaaac atttagaggt gagttaaata aattagctta
      841 tgagcaaaat atagtggata aatcaggtga gatttataga ttccatgccc atgcctttcg
      901 ccatacagta ggaacaagaa tgattaacaa cgggatgccc cagcatattg tgcagaaatt
      961 tttggggcat gaaagcccag aaatgacaag cagatacgct catatctttg atgaaactct
     1021 aaaaaatgaa tttactaaat ttcaggaaaa actggttacc aataatggag atgtgcttga
     1081 tctagatgaa gataatgaag tcgatgatgt agagcttcaa tggttcaaga aaaatataaa
     1141 tgcacaagtg cttccaaatg gttattgtag attgccagta gtagcaggtg gttgtccaca
     1201 tgcgaatgca tgcttagatt gcactcactt ctgtaccagt aagcaattct taccacagca
     1261 cgaagaacag ttagagcgta cagaagagtt attagccata gctaaggata aacaatggca
     1321 aagacaagta gagactaata gccgtgttaa agagcgttta gaacaaatca ttggaagttt
     1381 gacggggtaa ttatcaatgg ataaacaagt tagaaataca acagaaattg tacgtttggc
     1441 gaagcagaaa tcaaaaaaga caagggaaaa agtagacaaa gcgatttcta aattttcgat
     1501 tgaaggtaaa gttattaatt ttaattcaat agcaaaggaa gctaatgttt ctaaatcatg
     1561 gctttataag gaacacgata ttaggcaaag aatcgaatcc cttcgtgagc gtcaaataac
     1621 agcaaatgta gtctcaaaac ccaagaaaag ttctcgttcg gaggaaatcc ttattaaaac
     1681 cttaaaaaga agagtaatgg aattagaaaa agaaaataaa aaattacaga accaaattca
     1741 aaaattatat ggagatctgt ataataaaga ataattatta atctgtagac aaattgtgaa
     1801 aggatgtact taaacgctaa cggtcagctt tattgaacag taatttaagt atatgtccaa
     1861 tctagggtaa gtaaattgag tatcaatata aactttatat gaacataatc aacgaggtga
     1921 aatcatgagc aatttgatta acggaaaaat accaaatcaa gcgattcaaa cattaaaaat
     1981 cgtaaaagat ttatttggaa gttcaatagt tggagtatat ctatttggtt cagcagtaaa
     2041 tggtggttta cgcattaaca gcgatgtaga tgttctagtc gtcgtgaatc atagtttacc
     2101 tcaattaact cgaaaaaaac taacagaaag actaatgact atatcaggaa agattggaaa
     2161 tacggattct gttagaccac ttgaagttac ggttataaat aggagtgaag ttgtcccttg
     2221 gcaatatcct ccaaaaagag aatttatata cggtgagtgg ctcaggggtg aatttgagaa
     2281 tggacaaatt caggaaccaa gctatgatcc tgatttggct attgttttag cacaagcaag
     2341 aaagaatagt atttctctat ttggtcctga ttcttcaagt atacttgtct ccgtaccttt
     2401 gacagatatt cgaagagcaa ttaaggattc tttgccagaa ctaattgagg ggataaaagg
     2461 tgatgagcgt aatgtaattt taaccctagc tcgaatgtgg caaacagtga ctactggtga
     2521 aattacctcg aaagatgtcg ctgcagaatg ggctatacct cttttaccta aagagcatgt
     2581 aactttactg gatatagcta gaaaaggcta tcggggagag tgtgatgata agtgggaagg
     2641 actatattca aaggtgaaag cactcgttaa gtatatgaaa aattctatag aaacttctct
     2701 caattaggct aattttattg caataacagg tgcttacttt taaaactact gatttattga
     2761 taaatattga acaatttttg ggaagaataa agcgtcctct tgtgaaatta gagaacgctt
     2821 tattacttta atttagtgaa acaatttgta actattgaaa atagaaagaa attgttcctt
     2881 cgatagttta ttaatattag tgacatttgc atgcttcaaa gcctgtcgga attggttttt
     2941 agtgaaaaga acacgatatt cacggtttac ccacttataa acaaaagatc gatacttttt
     3001 gtagtccttc tttgaaatca atggttgatg tcgttcaaga acaatcaata cagagtctac
     3061 acttggctta ggatgaaaat atagtggtgg tacttttttg agcattttta tatccatctc
     3121 caccattaat agtaaaccca aagctcgttg cagattttgc aatcttttcg caaatccctt
     3181 ctcaacgata agatagctat atttagcctg actttcaaag gtaattcttt tgacaatatc
     3241 cgtactgatg ttataaggaa tattaccata tatcttatag tttatatgtt ttgggaagga
     3301 aaattttaga atatccgttt gaatcacttt tatattctca gaggggttta ccgcttcttt
     3361 agtcacttga cataagcctc catcaatttc tatagcaata actgatcgac tcattttgac
     3421 tagctctttg gtaaaatgtc cttttcctga tccgatttct attacgttgt cttgtttact
     3481 gatattcgtg tgattcaata tttcttttac atgcttttta gaagtaataa aattttgcgt
     3541 gtctttaggg tttttctggt tcattataac cttctcctta ctggttataa tgaactgaca
     3601 ttaagcgttc attataacca attaattttg atttggttga taacgaactt tattaataac
     3661 aaatatagaa aaagtaccca tacttttatc tcctttccat ttttttcatt atgaatgaga
     3721 taaagtaatt tctactactg cgatactggt gcacataatt gaaacctcct tatgtaactg
     3781 aatatgattg tatcatcttt tcattttaga ataaaccttt atatgtaatt ttgcacttga
     3841 cacttcagat ggacgttttc ttcaaaataa tatgttttga aacttgataa gagaagccac
     3901 tgtctttact aaaaagtaaa ggctgcgata aaggacagtg actttaataa agttgcactg
     3961 ttgtattgtc tttggataaa attgtctaaa gggaagtttt atccccaacg aaaaaacatc
     4021 taaagtggta agtaatgaaa gaatgtcgat aaagtaggaa tgggggatct ttttgaatac
     4081 aatagaatca cagtttgata aggttgcaga agattacgat ttcgtgaatg agcttttgaa
     4141 tgattattca ttctttgtgt ctaatatgtc tccaaagaaa ggcagagcat tagatatcgg
     4201 atgtggctcg ggtttgttag tggagaaatt agcaagttat tatgatgaag tggtagggat
     4261 tgatatttct aatcaaatgc tcgatcttgc caaatctaaa cgtcaactaa caaatacggt
     4321 ctatctgaat atgaatgcag aacaacttaa ttttaatgag aagtttgatt ttattgtaag
     4381 ccgaacaacc tttcatcatt tggatgatat agccagcgta atacagcaaa tgaaggaact
     4441 gttgaatgag gaaggaagaa tagttattct tgataatgta tctgaagtcg agacaccgcc
     4501 tacctatgtt tataaattag gggcaatcca agagttttta ccgcattgtt tcaaatttgg
     4561 aataaaaaat gcgattagaa tttataatca caacacatct aaatcatggc ttgagcattt
     4621 agcttcagat aagtacctat ctgaacaaaa ttattacgat ttatatgaaa agttgttgcc
     4681 tggatgccaa ttccataaaa tgggttgggc aatgggggtt gtctggacaa aataacttaa
     4741 agctgtggct ttgaaaagtt gaatttgtga ttgttttcag agctatcggg tgctttagta
     4801 taaataacaa tgaataaaaa atcagcctgt atcttatgtt actattaa
//
LOCUS       DAEGIQ010000158         4793 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000158 DAEGIQ010000000
VERSION     DAEGIQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.164"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="KMT45_002694"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="KMT45_002694"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBE7006118.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="KMT45_002695"
     CDS             1085..2005
                     /locus_tag="KMT45_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE7006119.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="KMT45_002696"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="KMT45_002696"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBE7006120.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="KMT45_002697"
     CDS             3311..4789
                     /locus_tag="KMT45_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7006121.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1687 a          772 c          925 g         1409 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt att
//
LOCUS       DAEGIQ010000159         4792 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000159 DAEGIQ010000000
VERSION     DAEGIQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.165"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(132..1940)
                     /gene="pepF"
                     /locus_tag="KMT45_002698"
     CDS             complement(132..1940)
                     /gene="pepF"
                     /locus_tag="KMT45_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBE7006122.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKRLNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2116..3114)
                     /locus_tag="KMT45_002699"
     CDS             complement(2116..3114)
                     /locus_tag="KMT45_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBE7006123.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3340..3993)
                     /locus_tag="KMT45_002700"
     CDS             complement(3340..3993)
                     /locus_tag="KMT45_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBE7006124.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4325..4723)
                     /gene="spxA"
                     /locus_tag="KMT45_002701"
     CDS             complement(4325..4723)
                     /gene="spxA"
                     /locus_tag="KMT45_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBE7006125.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1411 a         1008 c          752 g         1621 t
ORIGIN      
        1 tacaaaaaag atgttggtga agttcaccaa catcaaaaag tatagagcct taaaaaaata
       61 cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga gaaaatcaaa
      121 aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg ttgttcaaac
      181 atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc ttttttcatt
      241 acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt ctctagtgcc
      301 tctggttcct ggtttagtct tttcttcgcc agtgctgaag cagcagaaaa gccagtcgag
      361 tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc gaatttaatt
      421 tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc ataactatca
      481 cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat gaaatgctcg
      541 aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa gtaatggttg
      601 agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc cgttaggata
      661 ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc gtacacataa
      721 ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg gacaagcgta
      781 aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt gtcatagctt
      841 cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat ccaacgttca
      901 gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag tgcttctaaa
      961 gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa gaccggtgta
     1021 taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa ttccatgtat
     1081 cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta tacactttca
     1141 ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg gactttcgct
     1201 ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt tctaaattgt
     1261 tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc cgtgctttct
     1321 agcaactgac catacacgcc atgagataat tggactattt caccattttc tccttcaatc
     1381 gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga agcatcaaag
     1441 atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg ggctcgatta
     1501 tctactattt gctggatata atggcgatag acttccaatt ttggttcttc tttgaaatat
     1561 tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga aacggcttca
     1621 ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc tgtatttcca
     1681 gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg gcgataaacc
     1741 ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag tgttcctttg
     1801 tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc aaactcttgg
     1861 tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc ttctcgtttt
     1921 ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct atctatcaaa
     1981 atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc attcatgtgt
     2041 ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc gattcctagc
     2101 ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt taaaaaactg
     2161 ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat gttcttttat
     2221 ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt cttcgtacaa
     2281 cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt tttgatagat
     2341 ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt gtatggatac
     2401 tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat cttcactcac
     2461 tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata tttctttaag
     2521 gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg atacagtctc
     2581 tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc agcaatgcac
     2641 tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt gcgattgtcc
     2701 aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat aattttgggt
     2761 tctttcaata aatctctggt gagacagtct agaacattgg atttctattg ctagattcgc
     2821 caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa atactggttc
     2881 tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct cttttgattc
     2941 tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag cacatttttt
     3001 ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt tctttccttt
     3061 ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca gcatttttct
     3121 ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg cgtgaaaaag
     3181 ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata tttttcgttt
     3241 ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa aaaagactgc
     3301 gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat aaaatttcgt
     3361 tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg tttctggtgt
     3421 acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta catccgcttg
     3481 gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga ataaatacaa
     3541 atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt gttcaaaatc
     3601 ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag ataattcttc
     3661 ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt ctgaatcaac
     3721 agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga taaacagttc
     3781 taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc ggaactcctc
     3841 ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt tgtgattgcc
     3901 aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa ttagtacacg
     3961 gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt tgccacaatg
     4021 tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa atctcgctgt
     4081 taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt atttaactat
     4141 aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc cctctctatt
     4201 tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa tactttttgt
     4261 aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt ttcgtattat
     4321 ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt acatcgcgcg
     4381 gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta tcatcaatca
     4441 tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat tcttgtaacg
     4501 gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc gagatgattt
     4561 cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg ttcaatggtt
     4621 ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac gcacgagcct
     4681 ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac cactcctttt
     4741 cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc tg
//
LOCUS       DAEGIQ010000160         4605 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000160 DAEGIQ010000000
VERSION     DAEGIQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.169"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            194..2134
                     /locus_tag="KMT45_002702"
     CDS             194..2134
                     /locus_tag="KMT45_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBE7006126.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLTVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTAVHQLLSEM"
     gene            2221..4143
                     /locus_tag="KMT45_002703"
     CDS             2221..4143
                     /locus_tag="KMT45_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBE7006127.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGIGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIVQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQDETAYLTIHFQAAIERH
                     KHQKSRRYQAVLVSEYSKAMATFLEARLNRELPELTIIDLIEYKPELEREDFPPVDFI
                     LATLPFKHENIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LEWTDPVDILNFMGNVLAEHHYVEPEFVDSVLERDRHASTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPILWHGEQIRIVLLLAIKRKDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
BASE COUNT         1519 a          769 c          948 g         1369 t
ORIGIN      
        1 tagatgtacg taagcagaag tacagaaatg taagattttc caatattatt ttattcaaca
       61 taataaatcg tatatcaatt tttttagcga aatttctatt tattccaaga atttcagtta
      121 ttacggaatc attttgtgct taataaaaag aaaccgtttt tatatactat aaatgtagaa
      181 aggcggtagg aaaatgaaaa aaattattgc aatcacatcc tgtccagttg gtattgccca
      241 tacatacatg gcagcagaaa acttagagaa agcggggaaa gcagttggcg ccgaggtaaa
      301 agtcgaaaca catggttcca tcggtattga aaatgaattg actgcaagag atatcgaaga
      361 agcagctggt gtaatcatcg cagcagatac taaaatagat aaatcacgat tcggcggtaa
      421 acctttgata accgtaggtg tgcaagaagg aatccaccat gcagatgagt tggtgagaga
      481 tattttagca ggaaaagcgc cagtctataa aagtacggag gcaattattt cttccgaaaa
      541 taaaagtgaa tcagaaaatt taggtaaaaa aatctataag agtttgatga atggcgtttc
      601 gtacatggta cctttcgtag ttacaggagg gttattgatc gcgatttctt taacattagg
      661 aggaacagct acacctgaag ggatcaagat accagaagga acgatttggg caacgatgaa
      721 tagtatcgga agtatcgcta tggggttgat ggttccaatt ctatctgcat ttattgcgca
      781 aagtattgca gatcgtccag gacttgttcc tggatttgtt ggtgggatgt tagcggcaaa
      841 cggagcatta tatggtagtg atgcgaatgc gggattctta ggcgggatca tcactggatt
      901 tttagcagga ttcattgctt taggaatcaa gaaagtgcga gtaccgaaag ctttacaatc
      961 gatcatgcca attattgtta tcccaatttt aggatcattg acagttggtt ttgtgtttat
     1021 ctatgttatt ggagaaccag tagctcttct atttagttca ttgactcact ttttagcagg
     1081 gatgcaagga ggaagcgaaa ttgttctagc tatgatctta ggagcgatga ttgcttttga
     1141 tatggggggg ccgttcaata aagtagcctt tttgttcgga tctggcttga ttgcagaagg
     1201 caattattcc attatggggc caattgctgt agctatctgt atccctccat tagcattggg
     1261 gctggcatca ttgattttaa aaaccaaatt tacgaaaaca gaacgagaag ctggtaaggc
     1321 gtccttagca atgggactgt ttgggattac agaaggcgct attccgtttg cttcaacaga
     1381 tccactacgg gtgattccaa gcattgtaat cggggcaatg acaggaagtg tgatcgcgat
     1441 gctttcagga gtgacagatc atgtagctca tggcggtccg atcgtagcgg ttttaggagc
     1501 agtggatcac gtagccatgt tttttgtagc agtgattgct ggtattgctg taaccgttct
     1561 aatgttaaga atccttaaac cgacattaca acaaactgcg gttgctgcag caaatggtgt
     1621 gacagtagca gatctatcag agactgaaac aagtgatgtt tatacgaaaa ctgaaacttt
     1681 tgattctatc acggatatta tgtcagatga agcaatcgtt ttaggtgaaa cagcaacaac
     1741 aaaagacgga gtaatcgaag atttagtcca acgtttagaa aaaacagcgg cgatcacaga
     1801 taaaaccaga tttaaagaag tcatctatga tagagaacat gaaagtacaa cgggaatcgg
     1861 tatggggatt gctattccac atggaaaatc agcttatgtt cttaaaccaa cagtggcatt
     1921 tgccagaagt caatctgggg tagattggca ttctttagat ggaaaacctg cacatatgat
     1981 ctttatgatt gctgtacctg aaaacagcca aggtgatatg cacttgaaaa tactgcaacg
     2041 tctatcacgt aaattgatgg atgatgactt ccgtcaagca ctgatggaag cgccagataa
     2101 aacagcagtt caccaattat taagcgaaat gtaacataag atgaaaaatc gaccgtgatg
     2161 agacataata gatgtgttat catgatcgat tttttattta gaagggagtt tctgcacatt
     2221 atgaatcagc gtcagcaatc tttgatctat caattaagtg agaacaataa ttatataaca
     2281 acaaaaaagc tagcagagca tttccatgtg tcagaaagaa cgattcatag cgatttgaat
     2341 accattgaaa cttggttgag agacaaacat ctgcctttaa tgtttgaaag aaaaaaaggt
     2401 ataggtatct tgttgaatgg tctgcctcac gaaagggaac aactgaaacg agcgcttaat
     2461 gaagagactc aaacagaaat ggataaaaaa cagcttcgtc agttattgat tttccatctg
     2521 ctagtagcaa aagacggatt tagtcttgaa gaactttcag aaaaactata tgttggaaaa
     2581 cgtacgattc gtaaagaatt gacagagttg caaagctttt ttgagtatca ccatttacag
     2641 ctagtatcca aaacaaaact aggaactttt cttaaaggcg atgaacaaga aaaaagacag
     2701 ttgcttgtta agacattacg aaatatgcag aaagaagatc cgcaaagtcc gtcgttgaaa
     2761 gaattcttcg ctaaagatac attaaaagtt atccaacata cattacaaga tgttttttat
     2821 gaaaatcatt tagaacagcc gaatggactg gctagtgtgg agattcacat ctactttatg
     2881 ctcgagcgaa tgaaacagta ccagaaagtg aaattaagta aagacgaaaa cgaagtcgtg
     2941 gaacacaccc aagcacaaaa gttaagtagt caaatattag caaaactagc caccatctat
     3001 ccaattgaat tttctcctga tgaaatcaat tatctggctt tgcgtatcgc caatcttttt
     3061 acaaattcac aagcagccac acgttttcaa aaggaatcta gcgcacttgc tgatcatttg
     3121 attgtacaag tggagcagtt tttaggttat tcattgaaag aagatcagtt actgaaacaa
     3181 aacttaaggt ctcatttatc ttcgacctat tttcgactcc attatggctt gcagatttct
     3241 aatccattga cgaaaaacgt atttagtacg tacacacaac tttttttagt tttacagttg
     3301 attctagaag attattttga gaaagaaaat ttttatgttc cgcaagatga gacagcttat
     3361 ttaacgatcc attttcaagc ggcgatcgag cgacacaaac atcagaaaag tagaagatac
     3421 caagccgttt tagttagtga atattcgaaa gctatggcta cttttcttga agcacggctg
     3481 aacagagaac ttcctgaatt aacgattata gatttaattg aatataagcc agaactagag
     3541 agggaagact ttccacctgt tgattttatt ttggctacat taccgtttaa acatgaaaat
     3601 attccagtaa tcgagatttc acccatgatc acagaaactg atctggccta tttgacaaaa
     3661 tatatgctgg aacatgtacc tatcaagaag aaaaaaacgt ttgatctagc aagctttact
     3721 catccatttt tgatctttcc tcagcttgaa tggactgacc cagtagatat tttaaacttt
     3781 atggggaatg tcttagcaga acatcattat gtggagccgg aattcgtcga ttctgtcttg
     3841 gaacgagacc gtcacgccag tactagagtg gcaccatttg taacgattcc tcacggtaat
     3901 ccgctgtatg taaaacattc gatgatttct attgcaacaa tgaaagaacc gatcctatgg
     3961 catggggaac agatacgtat cgtactgctg ttagcaataa agagaaagga tttgaaacat
     4021 gcagagttca aaaaaatttt ttctgttatt cattatttag aaaagcaacc agaacaaatg
     4081 gcacagttga tgcaatccaa tcatccacta gagattttaa cgttgttgtc tagttatgaa
     4141 taagtagata ttaaaaaact gcctttatat ttataataat tctatctatt tatttgaagt
     4201 tgaaaaacta aatacaaaaa aaggggatgt ggcccaagtt ggtcacatcc ttttattttg
     4261 agtttcttat aatttttaat agttgcttac attgtcttga ctactggtaa acggtgttca
     4321 agagacaatc tgtcggcaat aaggcacaat tttgtaaaat atggaaaact attttctaac
     4381 cttgtttctt agtgcttgaa aaatcatttt gaatactttt ttatacaatc gatgtacgtt
     4441 gatttaaatc taaaactttt cttttgatta cttctctgac ttttctgaat tccgctaata
     4501 tttcttcttc gctcccgaca gcttgtgcag gatcttttag gtcccagtga aggtgttcgt
     4561 gattgtttgg aacggcaggg cacttgtctt tggcatcacc acaaa
//
LOCUS       DAEGIQ010000161         4595 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000161 DAEGIQ010000000
VERSION     DAEGIQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.170"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            361..1383
                     /locus_tag="KMT45_002704"
     CDS             361..1383
                     /locus_tag="KMT45_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBE7006128.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKTAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGVKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKVVTRERSLEFLELLEGKPFAEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            complement(1508..1942)
                     /locus_tag="KMT45_002705"
     CDS             complement(1508..1942)
                     /locus_tag="KMT45_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBE7006129.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYEHSH"
     gene            2057..3031
                     /locus_tag="KMT45_002706"
     CDS             2057..3031
                     /locus_tag="KMT45_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE7006130.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            complement(3552..4505)
                     /locus_tag="KMT45_002707"
     CDS             complement(3552..4505)
                     /locus_tag="KMT45_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBE7006131.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFASPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKQISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLANKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
BASE COUNT         1479 a          818 c          905 g         1393 t
ORIGIN      
        1 actgtttatc taaagaaaac tctatatgat ggagcgaaaa aaacaatggg tttcttagtt
       61 tatatgtttc ttgttgttca gcgtataaat tgatgtgtac aaagaactca tcctgtcttt
      121 tttaccataa atacacgtta gaaacaatca ttttgagtca aaaaacaacg atgggaaaga
      181 ctattaaaag agattaagac atggcagatg atgaagaaag cagggtgatt gtaagccgat
      241 agatttcttg aatatgacgc gagaacaatt attcgtcttt taagaaaaaa agtagtaaaa
      301 tcagttcata ttaaaaataa aaaaccttcc atttttgtag tattggagga ggtcaatacc
      361 ttgaaaaaaa tcggcttttt aagctttgga cattggatgg agcaaggatc gctcgtgaaa
      421 acagctcaag atgcttattt gcaatcaatc gatttagcag tggaagcaga aaaaatcgga
      481 gtggacggag cgtttttccg cgtacatcat tttgcccctc agattgggtc gccgtttcct
      541 ttgctagcgg caatcggcgt aaagacaagt aagattgaaa tcggtacagg acttattgat
      601 atgcgatatg aaaaccctta ttatatggca gaaaatgccg gattgacaga tatcatcacg
      661 caaggaagag tgcaactagg agtggggcgt ggttcacctg aacaagttct agatggctgg
      721 aaatactttg gttatgatta cagtgaagaa gaaatcaaag tggtaacacg ggaacgcagc
      781 cttgaattct tggaattact tgaaggaaaa ccatttgcag aaccaaatcc tcaaccaatg
      841 ttcccacaag gatcaggaaa attacggata gaaccttatt cagaaggatt acgacaaaga
      901 atctggtggg gagctggttc gcagcaaacg gcaatctggg cagcacaaca tggaatgaat
      961 ctgcaatcat ctacattagt caattacgaa tcccatgagc cattccatat ccaacaagca
     1021 aaccaattac gtgcgtataa agaagcgtgg aagcaagcag gtcatgattt tgaaccaaga
     1081 acattagtta ctcgctcgat tcagccaatc accactgatt tggatcgacg cttatttgga
     1141 caagacggta gtccgcaaca agatgctgtc ggctatcttg cttatcaccg gaacccgacg
     1201 atttttggaa aaacttttgc tggtgaacct gatcaattaa tcaaggagct agctgaagat
     1261 gaagcaatca aagaagcaga cacagtactt gtcacgattc ctaatacttt gggggtagaa
     1321 tacaacatgc acttgcttga aacaatcgta aaagatatcg ctccagaatt aggatggcgg
     1381 taaaaaggat atttctgtaa gtgcaggata cttgagtgaa aaaataaaca cttctaacaa
     1441 tcagttagag gtgtttattc atgttttcat gaagttcata gagaaggaaa gcgagttatc
     1501 cgtttgttca atgagaatgt tcataagctt cttttacatg ttggggtaaa tcatcaggaa
     1561 tcattgtcaa tacggcttct tcattgccgg cttttattgc ttcttcatag taccagcttt
     1621 tgtagtttag catagcgagt actctttcta attttgctat ttctttttct actacttctt
     1681 tttgtttttc aaatagttct ttacgaacct caaatgtctt actgccttct ttgctccatt
     1741 ccatgaattg cttaatgtct tttatttcta aaccagattt tttcaaacag tcaatgactc
     1801 ttaaagcctc taactcattc tctgaaaatt ttctaatacc agaaacacgc tggatatcag
     1861 gaaataatcc ctctttgtcg taataacgta aagtagaaac aggcaatcca aacatttcag
     1921 acacttgtcc aatggaatac ataaaaaaat cctcctaaaa taaaattttt accttgacct
     1981 aaagttaagt ttaagtttta tcataataat acattcaaaa gcaaatgtca attttgatca
     2041 aaggagagtc tagaatatga ataaagaatt aaacatcaca caagaatggg acaaagtatt
     2101 tccaaaaagc aatcaagtca atcatcgaaa ggtgagcttc ccgaatcgtt ttggtatcac
     2161 attagtcgct gatttatatg aaccaaaaga agtaagtgaa aagaaattgc cggcaatcgc
     2221 tgtgagcggg ccatttggtg cagtgaagga acaatcgtct ggtctatatg cgcagacgat
     2281 ggctgaaaga ggctttttaa cgattgcgtt tgatccatcg tttacaggag aaagcggagg
     2341 agtacctcga tatgttgctt cgcctgatat caatacggaa gatttccaag cggcagtaga
     2401 ctttttatct gtacaaaaaa atgtaaaccc tgagagaatc ggaatcattg gtatttgtgg
     2461 atggggaggt atggcattga atgcagcagc tatcgatacg cgggtcaaag caacagttgt
     2521 ttccacaatg tatgacatga ctcgagtaac agcaaatggc tacttcgatg ttatggatga
     2581 aaatgcccga tatgaaatgc gtgaagaatt aaatgcacaa cgcatcattg atttcaaaca
     2641 aggggactac gcacgtgctg gaggtgtggt tgatccgttg cctgaagatg ctcctcaatt
     2701 tgtcaaagac tatcatgcgt attataaaac agaccgcgga tatcatgtcc gttctttaaa
     2761 ctccaatgat ggttggaata ggacatcagg gttgtcactt atgaatgcaa aactattgca
     2821 ttatattcca gaaatccgtc atgcagtctt agtcattcac ggggaaaaag ctcattcacg
     2881 ttactttggg gaggacgctt tcaaagaact aaaaggtgac aacaaggaac tcatgatcat
     2941 tccgggagca tcccatacag atctatacga ccagacagac atcattccat ttgaaaaaat
     3001 ggcaacattc tttgaagaga accttgtgta accaaaagga tttaatctaa aagagattat
     3061 gaaaaaactg ccctgcatca agtaataagg acagccaaaa aagctcctca tttttgactt
     3121 ggttggactc attagcgcag atgcaagctt ttgagcaccg tttaaagaag atgttgtgaa
     3181 aaagccgctt agtttcgagt attaagaaaa attttgtgaa aatggctttc cacatttttg
     3241 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttatac caagaggtcg
     3301 tgagcctgcc gtttaactct gagtcacaag tagcaatttc aaaaaatagc ttctcatgtt
     3361 tgttgaatat tgtgacttgt gaccgaggag ttggcatgag aacaccgttt attcgaaaaa
     3421 aggactgtga cactgtaccg actcccaaaa gttagaccaa aaaatctaac ttttgggggt
     3481 cggtacacac gttttttgtc acagtctctt tgtgtctcat ttcatataaa caattattta
     3541 ctttgtacta tttatttcat ttctgtgatt ttaatgcttt ttgctaaatt ttttacatca
     3601 gctggaataa ctttcccgac ttctgattgc tgtgcattcg acatgccgca agccattgcg
     3661 taccttacac attcttttaa aggataattc tgttctaatg aatgagcaaa acctccaaca
     3721 gtggcatccc cagagcctgt tgtattaatg ataggaatct tagggatctc aacattccaa
     3781 aattcttctt ttactttgac tactgcacca tacgagccgc aagaaatcat gataacatca
     3841 atatctttaa atagtggatt tgtttttaga tggtgtttta tgtcgctttt ttgagtaatt
     3901 ttttgatgga tgatatctga aaactcatgg atattaggtt tgatgaacat cggtttatac
     3961 ttatttgcta agacttcacg gagatgttgt ccagatatat ccattaatat ataggtggaa
     4021 ggatctattt gttctttgat atattgtaat aatttagcat acaaaaaggg atctggattg
     4081 ttaacagaac catttattgt aatgactgaa aggtaattat ttttactgat ttgtttgata
     4141 tcattcatta attttttgat agtagtatct gctatttcag gaccagcttc cactagctct
     4201 gtctgcgttt catcatcatg catgatcgtg acggcacctc ttgattcttc tgcaataggt
     4261 aaaaaatgca aatcaaacaa attttcattt ttcagcatat cttgaatcat actgccaaaa
     4321 taaccgccca aaaaccccgt taaaataact gatgaaccta gtagagcaga agctcgtccg
     4381 caattgattc ccttgccgcc tacatagcgt accggagaag caaagcgtgt catcgtacct
     4441 aattcaaagt gttttgtaaa ataaatggaa tcaatggatg ggttcatcgt gattgtcagt
     4501 atcatttttt ggctccttta atatggttaa gtattttgaa taatgatgga aatcttgttt
     4561 tgataaatga ttatcggtta ttagataatc aaaat
//
LOCUS       DAEGIQ010000162         4517 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000162 DAEGIQ010000000
VERSION     DAEGIQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.171"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..859
                     /locus_tag="KMT45_002708"
     CDS             2..859
                     /locus_tag="KMT45_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBE7006132.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            879..1439
                     /locus_tag="KMT45_002709"
     CDS             879..1439
                     /locus_tag="KMT45_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HBE7006133.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            2174..2815
                     /locus_tag="KMT45_002710"
     CDS             2174..2815
                     /locus_tag="KMT45_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBE7006134.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYELTIV"
     gene            2934..4019
                     /locus_tag="KMT45_002711"
     CDS             2934..4019
                     /locus_tag="KMT45_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE7006135.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            4016..>4517
                     /locus_tag="KMT45_002712"
     CDS             4016..>4517
                     /locus_tag="KMT45_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006136.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQ"
BASE COUNT         1700 a          614 c          841 g         1362 t
ORIGIN      
        1 tatggaagaa aaattaatta aaaaggatga gattataatg gggttactgg ttccgagagc
       61 taatacttat actatacctt taacgaaacg ccaattaatt gctaaaagat atcaaaggat
      121 tactagagcc atcaatagag agttttggaa ttctgaaagt gatactgctc acagtttata
      181 cgtaggttct tatgggagag gaacggcaat tagtactagt gatatagata taatagttga
      241 attaccgatg gcagaatttg acagattcaa aaattattta tcaaatggcc cgtctaagtt
      301 attacaagtt ataaaaaacg catttcagga aatactacca aatagtgata ttagagctga
      361 tggacaagtt gttaaaataa atttccatga tggaatcaaa tttgaaatag ttccagcttt
      421 taatgaaaaa gattattggg gtgagtcaaa agggtttatt tatccagatt caaacatggg
      481 agggaactgg aaagctacaa atcccaaaaa agagcaagaa gccatgaaat taaagaatac
      541 aaaaagtaat aatttattat atgctacgtg taaacatttt cgacatgtac gagatacaga
      601 atttacgagt tatcatcttt caggaatagt aattgacagt ttcgtatatg aggctatggg
      661 aaattggaaa ttcgttgaaa ataacagtgg gggtcaaaac attagttctg tatcttatga
      721 gacagcttta ttagaatact ataactcaca taaagttatg ggcggtttaa atttatactc
      781 tcctggaagc aatcaatttg ttaattctga tagtagtatc atatgtttag aaaaagtatt
      841 aaaaaaaata gctttataaa gttttatgga ggaacaacat gggagacgaa aaaaagcgct
      901 tggaagttca attacgtgaa gcatatggac gagtaacgta tacttacacc actcatttaa
      961 agcaagccga taatattatt tctgacaata aaattttaag gtgtcttcaa attggtttgt
     1021 cagcccttac tactggaggg gtacttagta ccattattac tgataatagt gagttaacct
     1081 ttgttgctgg attatgttca acaatttctc ttggattaaa cttatacttt aagaattttg
     1141 atcttgctga agaagctaaa aatcatcaaa ttgttgccga taaattatgg ttgatcagag
     1201 aagaatacgt aagcttacta acagatttaa atcgtttaag tgttgaagat attgtgaaaa
     1261 aaagagatca attaatgaac gaaactcatg aagcatataa aaatgcccct aaaacaaata
     1321 gtaaaagcta tgaatcagct cagaaagcat taaaatctga agaagagcaa ttttttacag
     1381 aagaggaatt ggataagatg ttaccagagc atcttagaaa aaagagttct aaatattgaa
     1441 ctcttttttt atgcttaaaa ttctcttgta atttttctat tcatctatat ttaagaagaa
     1501 atatgatgat agaaacatct cctaaagcca ccgcaagccg ttctaagcat agcttagccc
     1561 gacattgcta gatatgctca tctcaccatg aacgtttcct aaagccaccg caaaccgtcc
     1621 tagccatcgc ctagtccgat tttgcttgat cggttccatt tctgtttatt tagtgggtca
     1681 acaccgtaat cttaaatagc catcgcctat ttaagattac taggtctgct ttgaaggtaa
     1741 aaagtctttc attcttcaag acttttaggc tttcaagcta cgcctgaaag cattgaaatt
     1801 catcgctctt tatttggttt tgccatttat aattccttac gcccattatc tttttcgcct
     1861 gtcatacctt gtcagaagtg tttagagctt atctcatctt tggattaata caagtggcgt
     1921 aaattttttt ctaaacgcaa gcgttacgaa aataatttac ttaaacactt gtattaatcc
     1981 ggcagataag aacgcattct aatcactccc gacaaggcaa gacaggcgaa aaagataaat
     2041 aaatgaacga aaggaagaaa cataaaatgc aaaaccaaat agcgatgaag aaaaaaccac
     2101 ctccctctaa aacaatccaa acaaaaaaga attttgaaaa accaaaaatc aaaaacagaa
     2161 agaagcgttc aacatgagag atagaatttt agaagttaat caagaaatgg taaacaagtt
     2221 aacaaatatc agtgggaact atgaaaagta cgatggtact ttacttgata attatatttt
     2281 tcacgataca caaaatatca gaattggcag aagtaaaccg agagcgttca ttctcatcaa
     2341 agagaaatat ttgaatgaat ggaatagcac ctatgaaatg gttatgactg ataactttga
     2401 aaaggtattg gatttcagaa atgctttgtt gggttctgat atgggaaata gcagtcttga
     2461 atctattgaa gttgaaaaca agaccgagaa tgaaggtgaa ggattgatta agaatgtcct
     2521 gaaaatcgaa caaaaatata tcagtgaaga tagaagtttt tatagagtca caagctcggt
     2581 tggtctagca aactttgcta ctaacttaat tggaaacaat gctagtgaat ctttgctagt
     2641 gatgtgtctg aatacaaaga atgaggtcat tgcttatagc gaagttttca aaggtggaat
     2701 taatcaatca gtagcgatac caagagagat ttttcaacta gctttgctaa acaattcagc
     2761 aaaaatcgca gtatcacata atcatccgag cggatatgaa ttaaccatag tataataaat
     2821 aatagtagtt aaagtagttt aagaggagat attaaaggga tattttactt taaataaaaa
     2881 ttaacaaggt ttataatttg tagtagttaa agtatttaaa gaggtgggaa catgtgaagg
     2941 ttcaaaggat agaagtggag aataagccgt atccattgta tttattacta gataaagaat
     3001 accagctaat agaaccagta atgaaattta ttaaatactt agataatact ggtaagtctc
     3061 ctaataccat taaggcatac tgctatcatt taaagttgct gtacgagttc atggaacaga
     3121 gaggtgttat tcttaatgat attaactttg agttgttagc agacttcgta ggttggttga
     3181 gatatccttc agcatcaaat gtaattgatc ttcagtcaaa aaaagccata agagaagaaa
     3241 cgacagtgaa tacaatttta aatgtagtta tgagttttct tgattattta agtagattag
     3301 gagaatttaa atcaattgat gtatttaaac aagcaaaggg aagaaatttc aaaggatttt
     3361 tacatcatgt taataagggt agataccaaa agaatgtctt aaagttaagg gttaaaaaga
     3421 aacagataag aacattgaga tcaaaggaag ttaagcaaat tattgatgct tgtcatacga
     3481 aaagagataa attaatttta atgcttatgt atgagggtgg tttaagaatc ggtgaagtgt
     3541 tatcgcttag gcttgaagat attgtcactt gggacaatca aatccattta acacctagag
     3601 atgttaatgt taatgaagct tatattaaat taaggaagga aagaacaata catgtgagta
     3661 aagaacttat gtcactttat acagattact tgatatatga gtatagtgag gaattggagc
     3721 atgattatgt ttttatttcc ttaaaagaag gctattttgg gaaaccacta aagtaccaaa
     3781 gtgttcttga tctagttaga agaatagtta aaaggactgg aatagaattt acatcacata
     3841 tgcttcgcca cactcacgca acgcagctaa ttagggaagg atgggatgtt gcgttcgttc
     3901 aaaagagatt aggtcacgca catgttcaga caacgttaaa tacctatgtt catctttcag
     3961 atcaggatat gaaaaatgag tttaataaat acctcgagag aaaggagcat aagaaatgaa
     4021 tgcttctagt aaaaggaaaa ttattagtca gagtgagatt agcaaaaaaa tagctgtaat
     4081 gaatgaagaa atgcaggggt tttgggctaa taatagttgg gatataagaa aatgtccaca
     4141 tccttctgcc atagaattaa gtaagaatcc tgctttaagg aatcgttggg ttcgttttga
     4201 acgtgttaaa aatctgtggt taagaacaga attgaaatat ttttattttt accatttaaa
     4261 caatggaata tggaatgcaa aaactgtctg gattagaaaa ggaacagtaa ttaataaaat
     4321 gttagatttc ttagatttaa agtatcctag cattacttca attactgaag ttcctattga
     4381 aaaagcaatg acggagtata gaacttattt gacaaaacgg ggtgttagaa ttaccactac
     4441 taattataag attactgcta atcaagaaaa aacacctgta aaagctaatt cctactatgt
     4501 tactaatcta aaacaat
//
LOCUS       DAEGIQ010000163         4464 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000163 DAEGIQ010000000
VERSION     DAEGIQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.172"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            573..1007
                     /locus_tag="KMT45_002713"
     CDS             573..1007
                     /locus_tag="KMT45_002713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006137.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2297..3220
                     /locus_tag="KMT45_002714"
     CDS             2297..3220
                     /locus_tag="KMT45_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBE7006138.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3236..3775
                     /locus_tag="KMT45_002715"
     CDS             3236..3775
                     /locus_tag="KMT45_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBE7006139.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1668 a          668 c          755 g         1373 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcacgcgctt
      241 atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt aaaaaaatga
      301 cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag ctttagaaag
      361 ttgcttttag ctagaccaat taaaaatcac aaagtgagcg tctctatcca ttggtagagg
      421 ggctacttgg catagtacac attatgtcta ctaaccttta aaataacacg ttttataaaa
      481 taaaccctct tgacaatatt taattttctg attattctaa atatatacag aaagcgcttt
      541 cctaggattt tatatctaag gaggaagtaa caatgaaaaa taaaattatt aaaattatta
      601 cttgtctatt tttgttcggt atgattgttg gagcaagtac tccgttaaat ctgtttcctt
      661 ttagtgctag aggtataaaa gtggaagctg caagttcacg atataatcat aatcatcgtg
      721 gatttacttg ctataaaatg aactattatg ttactaataa aatgtgtaga gatttgaaaa
      781 aaaattataa taagctaaag actcctgctc aaattgcatc ttttattcct attggtggag
      841 ttggaacatg ggttattaca aatgctttta gtagtgctgt tgatgcaatg aatgtctttg
      901 taagagctgc aaatcaagga aaaggtgtac aattaacata taatgctcac tttagcaata
      961 caacatcata tcaatacaat gattacgctc gttatgtaat aaaataatca gaaagtgaaa
     1021 ctatctcatg aataaaaaga ttttagctgt aattatttca ataatagttt taatcactat
     1081 gttcttcata tttaggctaa ttttcaatat aagcttacaa aaaagtattc tttatctaat
     1141 tcctatagca tttatcttag caatttttag aagtatttac ggaaataaaa actctaaata
     1201 gctaaaaata aatcatacta aattttacaa aaagtggcta gcattttcta gccacttttt
     1261 gtattgtgga gcgtgggttt tgagggggat tcaaggggga gcttgcgccc ctttgacaag
     1321 cggttatttg caccaaatac ggaggtattt ggctagcaaa tatagagctt gccaaaccct
     1381 tttagtgagt tttgtagaaa gtacctttaa aggtactttc acattacgaa cgatacgtcc
     1441 gtaggacgta ctgaaagggt gggacttatg agcaaattaa tgcaaaattt ggctagcaaa
     1501 aaagctaaaa aatatatgct aataatcgag cgaaataaac actttttcaa gtgagcgaat
     1561 ccgccatgac aaagctgaag cagtttattt cagctttgtc atggcgtgtg ccgacttttt
     1621 taaaagtcta ggcacacgag caaatatctt tatcaagctc taatttgcgt tttaagacgt
     1681 ttgaaacgcc taagtatata aatatataag aataggttta agagtgctta aatcgaattt
     1741 tagagggcat ttagacaatt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgt
     1801 tttaagaaca agataaggga tttatactag gactgtctta aaatgtctta aacgaacgct
     1861 cagagcttct caaagggttc cttatgaacg gcttttagca aaggagccgg aggcgactgc
     1921 gcagtataat gtgagagcag agcgatcaca cctttttagc gaaccgtagg gagcaaggtt
     1981 tggaattggc agttttgata aattcgcgaa cctgtttaga gaaaaaagga gtgatttttt
     2041 aggcgggaaa attagcctcc cctcttttta tatctctttt tatatctctt tttatatctc
     2101 ttttatgtcg gctgtgtcct tactctccca agcaattcag agttttttca agtaggtttt
     2161 ctacgctttt caagtaggtt ttctacgctt ttcaagtagg ttttctacgc ttttcaagta
     2221 ggttttccga cattattgag tatttttata aagtatgtta cttttatttc atactttata
     2281 aaagtaggag aagcttatgg caaatgaaat tgtaaaatat gaaaatagat taaatcatat
     2341 tcctctgaga aaatttaata gtcgtgaaat gaacttattt ttttcaattg cttcacgtgt
     2401 acgtgaaaaa ggtgtagatg agattatttt ttcatttcac gatttgaaaa aactaagcgg
     2461 ttatacagaa cgtggggaac aatttataaa agatcttagt agcacgtatg acaaattatt
     2521 aagtattaat gcttggaatg atgatggaaa tactttaact aaatttgttg ctttcacaaa
     2581 atatagcatt atacgagata aacaacttgt tataatttca gttaatccag aatttaaagg
     2641 actttttaat caattaaaga actggacaag atttaatcta gagcaattta ctaatttgga
     2701 tagcacatat tcaaaaacta tgtttcgatt attaaaacaa tatcgaactc aaggctggac
     2761 tgaattttcc aaagaaaaat tttttgattt actagatatt cctaaaagtt attggaatag
     2821 tccgtcaaat attgacaaat ttatcttaag gccaattaaa gaagaactca ctcccttatt
     2881 taaaggatta actattcgta aaaaatatgg taaaggtcga ggtaaacctg ttatcggtta
     2941 tcgttttact tggaaagctg aaattaacca tgctgacgat ttttccaaag ggaaacaaga
     3001 agatttacgt ataaaattat ttaatattga tcacaatgga gaattaacac gagaagaaaa
     3061 atggcgcgct aaagatcgaa tactaaatct tcccttaggc actcatgaag ctgattttaa
     3121 acaacaacaa caaactgaag aaaaacaggc agaaaaacga gaactttcaa acaaactcaa
     3181 acaagattta ctaggaaatt tacaaaactt attcgattaa gcttaaggag tatttatggc
     3241 agataaaaca atcaaagaac ttgcggaaga attaggagtt agtaaaacag caattaacaa
     3301 aaaagtttct gacaaaaatc gaaaactttg gtttgcaaaa aacggaaaca agtttgtgat
     3361 taacgaaacc ggtcaaaaag ctataaaatc aatgttttta gtcgataacg caaacttaaa
     3421 tcgaaaacta gttggcgaaa aatcaaaaac caaaaaccat aaaaacacca accaagtttc
     3481 cgaattaaaa ctcctttctt tcctagaaaa acagttaaat cgaaaagata tacagctaca
     3541 agaaaaagat aatcaaataa aacaaatgca aaaattactc gaccagcaac aacaactcac
     3601 tctacaagcg aaccaacaaa ttgagaacct acaagaacag ctccatttaa cttatacaga
     3661 agaaactcca acaaccgaat caactgcctt ttctgaaaaa aaagcagaca atacacaacc
     3721 acttattgaa aaaaagtggt ggcaaatttg gaaatctaat agaaccaatc tctaaaattt
     3781 tctagaagtt agttcggata gtagaggaaa aagttgtact acctgatggg gagatcactc
     3841 aaatttttat gagaaagaaa aaatagttta caactgtaaa caatcaatgt ttatagttgt
     3901 aaactatcaa actatatggt gacttattgc ttaaaatagg ttgaaatctc actcaatttt
     3961 tggtggaaaa acaaaataaa ttctaatcga ttttgttgaa aaagtcataa aaaaacaaat
     4021 gtgattttaa caaaatttca aaaaaatcgt ttgaaatttc gtttattttt tttgagaatt
     4081 ttgatcatcg tagggtcatt gtagaatcat tatgagatca tcgtgggatc atcgaataaa
     4141 gtatgggaaa ggcttgcggt gcttggtatt aagggcattc tccgcctccc aggttcttga
     4201 ccattttctc tcaacgtggt aaaaatccca cttataagag aaaatggtca agaacctaaa
     4261 cggtcggcga agaaaacata ccaagcacca caagcaaaaa aagttagagt aacttccaaa
     4321 aaagaaaaat tttttagaac aacttttgaa aatttaattt tgatttatat ccgttcaatt
     4381 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     4441 aaaagtggag gtataaccta tgtt
//
LOCUS       DAEGIQ010000164         4425 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000164 DAEGIQ010000000
VERSION     DAEGIQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.173"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            690..1658
                     /locus_tag="KMT45_002716"
     CDS             690..1658
                     /locus_tag="KMT45_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBE7006140.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="KMT45_002717"
     CDS             1669..2484
                     /locus_tag="KMT45_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBE7006141.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="KMT45_002718"
     CDS             2552..3262
                     /locus_tag="KMT45_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7006142.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="KMT45_002719"
     CDS             3298..>4425
                     /locus_tag="KMT45_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBE7006143.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAEGIQ010000165         4418 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000165 DAEGIQ010000000
VERSION     DAEGIQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.174"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            59..913
                     /locus_tag="KMT45_002720"
     CDS             59..913
                     /locus_tag="KMT45_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBE7006144.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="KMT45_002721"
     CDS             1010..3259
                     /locus_tag="KMT45_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBE7006145.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4418
                     /locus_tag="KMT45_002722"
     CDS             3262..>4418
                     /locus_tag="KMT45_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7006146.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1556 a          625 c          807 g         1430 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagt
//
LOCUS       DAEGIQ010000166         4298 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000166 DAEGIQ010000000
VERSION     DAEGIQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.175"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            106..357
                     /locus_tag="KMT45_002723"
                     /pseudo
     CDS             106..357
                     /locus_tag="KMT45_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="KMT45_002724"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="KMT45_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBE7006147.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="KMT45_002725"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="KMT45_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="KMT45_002726"
     CDS             1786..1869
                     /locus_tag="KMT45_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBE7006148.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="KMT45_002727"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="KMT45_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBE7006149.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="KMT45_002728"
     CDS             complement(2994..3857)
                     /locus_tag="KMT45_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HBE7006150.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="KMT45_002729"
     CDS             complement(3859..4131)
                     /locus_tag="KMT45_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBE7006151.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..>4298)
                     /locus_tag="KMT45_002730"
     CDS             complement(4148..>4298)
                     /locus_tag="KMT45_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBE7006152.1"
                     /translation="DIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMDQALEEYIRKY
                     LPDKL"
BASE COUNT         1340 a          718 c          812 g         1428 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatct
//
LOCUS       DAEGIQ010000167         4281 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000167 DAEGIQ010000000
VERSION     DAEGIQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.176"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(37..1257)
                     /locus_tag="KMT45_002731"
     CDS             complement(37..1257)
                     /locus_tag="KMT45_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBE7006153.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="KMT45_002732"
     CDS             complement(1277..1888)
                     /locus_tag="KMT45_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBE7006154.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="KMT45_002733"
     CDS             complement(1931..2863)
                     /locus_tag="KMT45_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBE7006155.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3283..4143)
                     /locus_tag="KMT45_002734"
     CDS             complement(3283..4143)
                     /locus_tag="KMT45_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7006156.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1218 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa acaaataagg gaaacaaaca acacattttt
     3061 gtgtgttgct tatttccctc taactatcgt tttctatttg atggttaacc tacttaaaat
     3121 aaatactgca acgacaaacg gagatcaagc atgctttgta acgtatttta cgataattac
     3181 gactttattt tattactcta tcttgctgcc atacccgtct atccctaatc atctttggtg
     3241 aattttttca aagttagcat aacaaaaata agtatttttt ccttagttaa tcaatagtat
     3301 catttttttc atatgattta attcatcaat taattttttg tttttattaa ttttagcaaa
     3361 agctagtgca tcttgaatca attcgagcac ttctgccttc gacttatttt tagcctgtgc
     3421 gttcttcgct aattgggcag cagctcgtgc taagtaataa gtcaaatggt tatccgaaca
     3481 aatatcgatt gcgtaattta gtagtgcatc acttgtttcc aaatcaccgt cttgcgcaaa
     3541 aaaaacacca caatgaaaga cgatatttaa cacccgccag atatcttctg ttgtacgaat
     3601 cggatacgta taaatttttt caaacactcg attgaaataa acttctgctt tttccaactc
     3661 tttttctcta gcatagacca ttccaatacc tgtatatgcc aataaacgaa acaacgtgtt
     3721 ggctgtttct gccatcaaca actgatcaaa atggaacaac acatccgtaa ttggtgcatt
     3781 cgtaaaaatc atttcaaatc cttttagata gtagtaacgt gcaatcagct cagactcgtc
     3841 aattaccttg ggatcaatgg ctgctaaaat ctgtgtcaat tgttcatatt cactcgtaat
     3901 cagtgaaaac tctgcttttt ctagctgttc atcaagttgt gtattgatca cgttcatcga
     3961 agaaaaaagt tcaactaacg gaagattgat tcgattacat aatttgatta aaatttttaa
     4021 cgaagggact tgaccattgt tctcaaattt acttaatgtt gcttgtgtac aaattccttc
     4081 agacacttct ttttgagata agttggcagc ctttcttgcc gtaataaatt tttcaatgtt
     4141 cattattttc tctccaatca gacctcacac gaaaatcatt tctgtctcga ttttagcatg
     4201 ttttctttgg gaatgcttta ggttctgttg caaagtttta aataaagaat aaagtccctt
     4261 acggtatcta tgatttaagc t
//
LOCUS       DAEGIQ010000168         4257 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000168 DAEGIQ010000000
VERSION     DAEGIQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.177"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            73..294
                     /locus_tag="KMT45_002735"
     CDS             73..294
                     /locus_tag="KMT45_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBE7006157.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            296..562
                     /locus_tag="KMT45_002736"
     CDS             296..562
                     /locus_tag="KMT45_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE7006158.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            675..1214
                     /locus_tag="KMT45_002737"
     CDS             675..1214
                     /locus_tag="KMT45_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006159.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            1422..1604
                     /locus_tag="KMT45_002738"
     CDS             1422..1604
                     /locus_tag="KMT45_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBE7006160.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAETYTSKATGLAVSS
                     FLTLLTFFVTLSFSFV"
     gene            1708..1992
                     /locus_tag="KMT45_002739"
     CDS             1708..1992
                     /locus_tag="KMT45_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006161.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            2266..2520
                     /locus_tag="KMT45_002740"
     CDS             2266..2520
                     /locus_tag="KMT45_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006162.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            2602..2787
                     /locus_tag="KMT45_002741"
     CDS             2602..2787
                     /locus_tag="KMT45_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBE7006163.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            2854..3249
                     /locus_tag="KMT45_002742"
     CDS             2854..3249
                     /locus_tag="KMT45_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBE7006164.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            3239..3616
                     /locus_tag="KMT45_002743"
     CDS             3239..3616
                     /locus_tag="KMT45_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006165.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            3638..4012
                     /locus_tag="KMT45_002744"
     CDS             3638..4012
                     /locus_tag="KMT45_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006166.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1531 a          705 c          756 g         1265 t
ORIGIN      
        1 tattcatata tataactatt gcatttgtca tacaatcgta gtacaatgta attgaaaagg
       61 agttgataaa ctatgccagt aataagtatt cgtgtatcta aagaagaaaa agattttctt
      121 gaaaaagttg ctgaattcaa cggagataac atttctgaat ttatgcgtag taattccctg
      181 caaacagcag aaacgttagt agactttgcc acctacaagg aactaatgaa agagcatgag
      241 gaaaacgatc aaagtatttc ccacgaagat atgctaaaag agttaggctt ataagatgac
      301 ttaccaagta agatatgaac gtgaagctca aaaagcgcta aaaaaaatgg ataagtttca
      361 ggcaaaggtt atcctaaact ggatcgagaa aaatcttgta ggcactgatg atccaagaaa
      421 acatggtaaa ggtctaacag ctaataaatc aggctactgg cgatataggg taggtgccta
      481 ccgtataata gctgatatta gcgagcatga agtgacaatt cttatattgt ctattggtca
      541 cagaagtgaa gtttataagt aacagacaag ctgataaggc ttgtctgttt ttttgttaac
      601 ccaacaaaat cactgtttac gatcccactt tacctaaaaa agaacaatac cctcttgtta
      661 ttatacacac tggtgtgtat aatataagtg taaacaagct aattaacttt caaaaggagc
      721 gattttttat gggatggtat ggtgcttatt catcagcaaa tgaagtgatc gaagaaaaga
      781 aagggtacgc caaaaatggt ttggaaatcg tagataccaa agtaacgaag acctatggta
      841 ttatcctttt caaagatgca gaaggaacct acgatattga cagcttcatc tttcgagatg
      901 aaatgtacaa accaatctac ttcttaaatg acagtcctgc ttactttaag aaaattccga
      961 aaaaatggtt gaaattcttt gtcggtgatc aagaaaaaat ggttaagcaa caactagata
     1021 gaattaaaga agaaaacaat aaaccaaacc ttgatgactt attagttgca ggtaaaactt
     1081 actcaatctg ggatggatcg tatgtcgctg tttatcagta taaactcaaa cgtagcttta
     1141 tttttagact acaagatgga tcactcactc ggttcactcg actatcagca ggagatgtta
     1201 aggaagtagc ctaaaactta aatacataaa cataaacgtg tatacgttta tgtttatgtt
     1261 tatgtatatg tatatgaata taaacgtatt aggtacgaac gtttttaaat gcttttctgg
     1321 gctttgattt tagggtaggg ctaaaacatc cattgatcca aaacagctca gaaacgcatt
     1381 taaacgtatc tgataccaat attacagaag gagtgtatcg tatgattaac ctatttcttt
     1441 tagtgacagc tagtacatta ttctttactg gaatatcact gataatttcc tatttgatct
     1501 ataagaaagc cgaaacttac acaagcaaag caaccggact agccgtttcc tcctttttaa
     1561 ctttgctgac tttttttgtt actctatcgt tttcttttgt ttagcacagc tgatccatca
     1621 gcaaaaaacc tctctttaca ttatacacac tagtgtgtat aatgtaattg taaacaagct
     1681 aattaacttt taggaaggag tgacaatatg ctaacagcta cattacagca acctactcac
     1741 gttgctattg cagaaagcta tacaattaat gcaggggtcg ttaaacagct gatcaaggat
     1801 cgagaattca aaaagtttaa cagtcgtcaa gcgtataaca gacagttaaa gaaagctatt
     1861 ctaaagcaca aaaattcctt catagggaaa ttggaaagtg tggatgttaa aactgtttca
     1921 acggcatttt cagttgttga agtgactatt gacggtcgct tgatttctct atttgctttt
     1981 ggaattaatt gatagggagt gtggactttg gaaggtaaga cttttgcaga aatttggact
     2041 gaatttattg aggtaccaac tcgcattgat ccgataactt ctgaaaaggt attggatgca
     2101 gacttctaca atctcaaagc aggtactagc cttgaatgcg tttagatgta tttagaagag
     2161 catttcaaag gcgtaacgct cggtcacgtc ttataccaca atggtgccga gtgggatgca
     2221 attggaaata tccatttttc ttaatacaag aaaggagaac acaatatgaa ttataccgat
     2281 gaagaacaaa tcactttgga atacgttttc acaaatgaaa aaggggagga aattgaaaaa
     2341 gtcttgatca tttcatcaaa agacttggag aacgctttga cttatgaacc atacgaatca
     2401 accgtaactg tcaatgaatt cttagatacc tatacttcta atgatacttg ggaaattatg
     2461 aatatttgca acgctaataa cattcaattt gctgaaaaaa ccaataaatc attttattag
     2521 ttttgcaata aaacatcctc tttttctatt gattccatac acaacaatgt gtataataga
     2581 ttttgtaaag ggagggtagc tatgcctaag acagcaagag aaattgtaaa aattctcaaa
     2641 tctaacggct tcaaaaagac tggacaaact ggcagtcatg caaaatttta taattccaag
     2701 actaaaagaa cggtcgttgt tccgatccac aatggtgatg taccactagg aacagaagct
     2761 aatatctgga agcaagcagg gctttaacag ccttgccttc ctatttggta ttaggcatac
     2821 tatcctctct tacactagaa aaggagcgag gaaatgaaga caaaaaatat tttttatccg
     2881 gcaatcttcg agaaagaagg agaagcttat aacatcgttt tccctgactt accggaaata
     2941 tctacctttg gaaatagtct tgaagaagca tatttaaacg ctaaggatgc tttaggatta
     3001 gcgctatata gtgtaccaga tgcagaattt cctaaaccat cagcagtgga agaattaact
     3061 ttgaaagcca accaagtggt agtaatcatc caactaaaca ttaaactttt ccgaagaagc
     3121 ttaaatacaa aaacaattag aaaaaatgtt agtgttccgg aatggttagt tcttttaggg
     3181 aaggagaaaa acataaactt ctctcaacta cttcaaaaag cactagaaga agagttgctt
     3241 gaaaagtaaa aaggagaccg aacctatgaa agctacacac ttaaactatt tctctcgaac
     3301 gttaatcaat aatctattaa aagatacaac tggcattagc gttagtacca aacactttgc
     3361 caatgatcta attatttttg aggataataa cttgcgaatt gagatgttta ttgaacaggc
     3421 aacccctaac tcaatacctg aattctgcta tacgcacact gaaaaaatga aacatgcaaa
     3481 atcagttaaa gatgttgaag cattaagtga atcactaacg aacgctttaa acctcgttaa
     3541 gaaggcaaaa ttgttatttg aatttcaaac cgactaccac aatgagttgc ttgctcaatc
     3601 tgaaatttta gattaattga aacaggagaa gagaaagatg aaacaaacac aaacagaaac
     3661 cgttaacgat acgcttgctg atcaattagc aggttattat aaattatttg agatgggtaa
     3721 agcacagcgc ttgatccact atctttggaa atacaaaatt ttgtttcttt tttgggaatc
     3781 aacactaaca ttctcagttc ttttttataa gtcagatttt tataatcaac acttagtttt
     3841 agggagtttt ttgcttatct acaccatttt gatgcttaat ttatatacct acatcacact
     3901 tgaagaacac ttagtaatta gcgatgcagg gatcgagaga aacatcaaaa gggtatcctt
     3961 caagatcacg ttaaacaagc ctaccgataa aaagctctca gaagcgttct gagagctttt
     4021 gacattttct tgtataattt tatcctaaca cattacaaag cacctaattc gagcttatat
     4081 caagcaaatt tgctatttat tactcaatac atatctaaaa attacttgat ggaaaattag
     4141 atacatggta agcatgaaaa gaaaatcaat ttgagcagat gcagtacaat acatagcaat
     4201 ttaaaagctc tcagaaggct tctgagagct tttaatatat tctttgataa tttgtct
//
LOCUS       DAEGIQ010000169         4169 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000169 DAEGIQ010000000
VERSION     DAEGIQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.178"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1043)
                     /locus_tag="KMT45_002745"
     CDS             complement(<1..1043)
                     /locus_tag="KMT45_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006167.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNT"
     gene            complement(1267..2151)
                     /locus_tag="KMT45_002746"
     CDS             complement(1267..2151)
                     /locus_tag="KMT45_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBE7006168.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDYYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(2246..2683)
                     /locus_tag="KMT45_002747"
     CDS             complement(2246..2683)
                     /locus_tag="KMT45_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006169.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(2900..3811)
                     /locus_tag="KMT45_002748"
     CDS             complement(2900..3811)
                     /locus_tag="KMT45_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE7006170.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         1196 a          861 c          655 g         1457 t
ORIGIN      
        1 tttgtattta ccgaaaattc gcttcccgaa ccactgactt caaacatgac attaccaggt
       61 agcgtatcga ctgcctctcc ttcatcgcta acgccatctg cccgatcata atcaagagag
      121 acctttgaac cgcttaagat atggattcct ggcgctgttt ttccactacc agtctcatct
      181 cctgaatcta gaaaaccgta gaatttccct agatacggtc cataaagttg actaccattg
      241 atcacattca attgatagct tctacaaaaa taggctttac ccggagcggc attcagtcct
      301 tccttcactg gttggacatc caacacaccg gaaacaccat ctaatgtgac agttgcacga
      361 ggagcgtaaa tcagtcctaa tttggaccca tcatacaaat caagtgtccc ggtaaatttt
      421 atttctcccg taaaatgata tctgcttccc caaaaatcgg tcatataagc aatgataatg
      481 gcatttccat cgatatttgg tgtcaatcca cctggatgtg cagctgttcc tttaaaattc
      541 tggaactcga tcgactgagg tatctcattg atataaatat ggtgtgtgcc gaaattgtga
      601 gcgaatccac caaaatcaat aacgatatct cgtttgatct ctaattccct gtctaaagtc
      661 aaaggctgaa tcagtcggat cttagttact gattcgtctg cgagaagctg cgccagttct
      721 tctgttgtgg cagcttcgct gtacccatcc gctgaaacag ctcttgccag tgccgcttca
      781 ctgctatcag acgtttgttc agctacttct gaggtctcac ttgtcttatt ttcttcttgc
      841 cttttattag tagaagcctc actttgagat tctgtagttt cagaagtaat cgattcttct
      901 gaaaaagtag tcttgtcaaa actttgattc tctgattgcg ttgattcgtt tgttttctct
      961 acatcggcaa aaacggatgt caccatcaag agagggaaga caaacaaaat cgacgcaatc
     1021 agctttttta cacattgatt cattctccac catcctctct gacgttttga caattttatt
     1081 ttatcgtttt aatctatttc caattgtact ttcatattta aaatatgtat ttttatggaa
     1141 aaatttttct attaggtgta aatttctttt ttgtacaaaa aaaaggctgt gacaaaagtc
     1201 ttgtcacagt cccttatccc gaacaagcgg tgttctcccg ccaactcctc ggtcacaacc
     1261 tccattttaa caataaacct gtaaatgtga gggcaatact ttgattgtca caggtaattt
     1321 atccccttca tctccatcaa cattcgtcgt caattcttcc tcatcagcta atgacacaac
     1381 taattgctca caagtaaggt aggctagatt tttcgtagat tcatctactc cttttaatag
     1441 gtctggcaat gttttaagcg tatcgatcaa agaagaatct tttaaataaa ccaaatggag
     1501 cttaccatca tccacttttg catttggaag taatgtttca aaaccgccga ctgagttagt
     1561 tagtcctatc agcaaggtac tgctctcaat ttcttccttc tttccatcaa cttctacgtg
     1621 aaacgagtag gattgtgtgg aagctaactg tttgattcct gacatgaaat aagctagttt
     1681 tccaaatttc gtcttcttct ccgcatctac atcattgatt gattcaggga tagagccaat
     1741 tgcgacgaca ttcataaaat agtagtcatt tatttttcca atatccaatg gcttgactga
     1801 ttcaatcgaa aaatgattga tcgcttcctc aggttctagc ggtatcccta atgctcgagc
     1861 caagtcattc accgtaccca atgggaaaaa tccgaaattt ggccgatgtt cttgttcagc
     1921 gattccactg attccttcgt tgactgttcc atctccgccc atgacaaaca cactatgata
     1981 cccttccgta gccgcttcgc gagtaaagtt ttttgcatcc cctgcttttt ttgtatgcaa
     2041 gacgaccact tcatcaaaca ccgattccaa ttttgctatc gctaattgct caaattcctt
     2101 tgcttgttca ccgcctgaac tggggtttac cactaataaa acttttttca tcactttctt
     2161 tcccttctga ttgatttctt taatataacg atagcataaa gaaaaaaatt aggctgtgat
     2221 gaagtactgt tcatgaaaca ttcatttatt ttttgacaag ataaaacatc catactatga
     2281 aactgaggaa aattaaaaca atgatatagg aaataaaaga aaatgatagg cattgtatac
     2341 ctaataaagt aaaaatctta ggtggcagat aaaaacacaa aaatagcaag agaatgtaat
     2401 agcgtttctt ccactttttg ttatcagacc ttatagcgat cagaggaata ccaaatgcat
     2461 aactgaaaaa ctcttgatgt actgttaata tgtcacggcc ttgaataata aatgcgacaa
     2521 tccccgctat aattagtgtg cctaacacca aaacggaaaa ttgatgaata tattttatat
     2581 acaaatgatc cattgtactt cctgcctttt tatttgtgag aaaaacaaat ccgtatacgt
     2641 gaataacttc tagaaataaa agaaatttgc aaaaccactc catttatcta cttctacgtg
     2701 cattttttga tattgttttc tttatttaag attatacaga aaaatatcta gggtcgctgg
     2761 attctaagta aaaaaaattt ttaaaataaa aagaactaaa tcaagtagta attaaactac
     2821 tgttgattta gttctaataa aaagatttag atgaacagtt ttattgattt aaatagttca
     2881 tattttttat ttataccgct tatttactac ttgctgtact agaatctgaa gtgaatgtac
     2941 tcaaaacatt ttcaaagtct ttgtctttga tttttacatt agcgtctttc aatacttcgc
     3001 caattacttt attctggaag gtactgtcac ttaattttgt gtctgttgca atttctttca
     3061 attctttttt gtatttatcc atgtcgttgc ctttgttttg gtttttcacc attttaacca
     3121 cataatattc cgtagtataa gttgaagcat tggtagaagt gatcacatca gaaacttgac
     3181 catctttcaa cttaaaggct gcttctttga cttctgccgg aaccgttgta gtagtggaat
     3241 caaatttcac ttttccgcca tcttcttttg tcgtagtatc agttgacttg tctttggcta
     3301 atttagagaa atcgtctccc ttatcagcag ctttcttcac atcttttgca tcatcttcac
     3361 ttgctacttg gataatttga gcttccactt ctggatggaa agattcccaa gccgttttta
     3421 aatcttcatc ggtcaatttg atatgtttct ttagaccttc ttgaaaggct agactttgtt
     3481 tgatactgtc tttgaatgtt tgttctgtgt aacctgcaga tttcaattgt gaatcaaatg
     3541 aatcacccaa ttgtttcttt gtttgatcat attgagaatc gatttctttg gttgatacgt
     3601 catctccata tttatcttca aatacttgat aaacgatcaa ctgttgcaca gcggaatctg
     3661 ctccttgcat ttttttcaag ctatccatta cttcttgttc gctgattgta ccgcctttat
     3721 aactaacggc tgttttttca ccggcattcg tacagcctga caataagcca gtgcctagta
     3781 cgatggcaag tgctaaaaat ctttttttca tttatcaatt ctcctacttt tcgttttttg
     3841 cgaattgcta aaaacaaagg tgttcaagat gaaaatcatc aagaacacct ttactaatct
     3901 gctcgatacg tcctgaatag atataaggct acacaatcca gaaagttcgc tcagcagaat
     3961 aaatgtcaag tagcacatca caccttaata tactacttct tttgttaccg tttttcaagt
     4021 ctatcttgcg ttccgtattt aacgtaacta attcttagaa caaatgttat cgattttgtt
     4081 tttcttcttc cgctgctggg atattcaaat cagacacttt tatgttcgat tttccagaca
     4141 tttttttgtt gtttttatta tgatatatt
//
LOCUS       DAEGIQ010000170         3980 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000170 DAEGIQ010000000
VERSION     DAEGIQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.179"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            402..734
                     /locus_tag="KMT45_002749"
     CDS             402..734
                     /locus_tag="KMT45_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006171.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
     gene            859..1116
                     /locus_tag="KMT45_002750"
     CDS             859..1116
                     /locus_tag="KMT45_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBE7006172.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            1348..1752
                     /locus_tag="KMT45_002751"
     CDS             1348..1752
                     /locus_tag="KMT45_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HBE7006173.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            1771..>3980
                     /locus_tag="KMT45_002752"
     CDS             1771..>3980
                     /locus_tag="KMT45_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBE7006174.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFS"
BASE COUNT         1468 a          529 c          724 g         1259 t
ORIGIN      
        1 cgttatgtag taaatgtaat agtttttttg gtagtgagta tgttgaggaa tatattagat
       61 ttgcagaaga caataaaaat ttcctttata acaatacaag tttaaagaat ggtaagtctg
      121 atttaacaca ttcaataaag aaaatgaatt cacttagagt tgcaaaagca attgtaggaa
      181 tgttttttag tttaaatggg gatgaagatt caatggataa gccattctta gattcggtac
      241 gagaatatct tagcaatcct aaaagtactt tatttcctca tgaagactac aaaattataa
      301 tgaattatta tcttccaata ggtgctccac agaattctaa aatcaaaatt cctaatgaca
      361 ttgtaaatga tggtgacact cacaatgtaa taaaaggttt tctgcccctt gatctatcaa
      421 ctgggagaaa aattcgattt tcagaaattc agtatgaagg aattggtttt actcttatag
      481 atttaaaaaa ttcaactttt gaaataaaag aaggattaga tttaaaaaat ctcctttgtg
      541 cagatgataa aatcaaaaaa atacaattgt acaaagtccc tatattaaca ccaacagtac
      601 ttacaatgtt ggagatggaa aataatatta attatattga ctcaggttca gatttgctga
      661 ttaggtggca ggtaataact aattttctta tggaattaaa aaaatacagt aatactcaaa
      721 cgaagttctc ttgaagttat aaagaataca ataaatataa atttccttta gactatatta
      781 aaatcagcaa tcgaaggaaa ataaaagaag aaaatggtac gcaaaatcta actattagag
      841 caaaaacagg aagctgcttt ggaagatagt cccgaattgt ttccgatgtt aaaagatcgt
      901 ttaaatgaat tgactgaaaa acgaagacag ttacaaattc gagagaatga aatcttaggg
      961 atctttcaac aacaagggga accgattcaa gtcaaagatg tccaacagat attgactagt
     1021 ttggattgct ttcttatcca gagtgagaag aagcaagtta aagctttgta tcacaccttt
     1081 attgagaaaa tcacctttga tcctaatcag aagtagatat tgccgatccc gatgagattc
     1141 acacctactg tgatttgata attgaatgag taatatcaaa tagccgtatc caaaccaaag
     1201 gatatggcta tttttgtgtt agggatatac gtcacgtagc ttgttatcaa gaataagttt
     1261 ctttattttt ttgctttttc taaaactgag tatgctaaaa taagagggta tatagcaact
     1321 ttaaataaga gataggagtg tatctttttg ggtgcagaat taaatcagaa gttatttagt
     1381 gcggcagata atctccgtag taaaatggat gcgtcagaat ataaaaatta tttattgggg
     1441 ctgattttct ataaatattt atccgaccgt ttgttggaac aagtggttct tttagcagat
     1501 gaatccttag aggaatatga cacagtaagc aaacaaacga tgttgtatcg tgaattactt
     1561 tcagatgaag aatcaaaaga agatctgatt gcaacaatcg ttgatatttt ggggtatgcg
     1621 atttcgccag agtatctttt caacgtctta gcggatcaag caaaacaagc aacgttccaa
     1681 ttgaatgatc taaacaaagc ctttttagca agtcatattt atggaaaaat actgtcggca
     1741 aaattcgaat agtgtcgagg aggtgctgtg atggaagaat taaaagaacg cattgtcaat
     1801 tatgtcatag agaactctga gattaaaaat aaatggggga acaatacagc acagaaaata
     1861 tctgaaaaat taaatgttag tcgtaatatt gtctctcaat atttgaatga attcgtaaaa
     1921 gatggagaat ttggaaaaat caatactcgt ccagtaatct tcttttcttt accggagaaa
     1981 gttccaaatc aacaaatttt tagctcttca gaggaagcgg aaaaatggta tgatagctct
     2041 tttagggttg gaatagaaag ggttatcgga agtcaaggaa gtctgcgaaa tgtaattgct
     2101 caaattaaag cagcaatcta ctatccgcca aatggcttac ccattttact tcatggcgag
     2161 actggaactg gaaaaagcta tttagcaaat tatgtgtata aggatttgtt ggccaataat
     2221 tatatagaag agaaggcatc tttcatttca gttaattgta gtgaatatgc caataatcca
     2281 gaacttttct tgtccaattt ttttgggcat gtaaaaggtg catatacagg agccgaagat
     2341 ggaagaaagg gtttagcaga attagctaat aatggagtct tatttttaga tgaaattcat
     2401 gcattgagca atgaatgtca ggagaaactt tttcagttta tggatacgag gaaatttcat
     2461 cggatgggtg ataatgaaaa ctggtacaca tcaagttgct ggattatatt tgcaacatca
     2521 gaaccacctg aggataaact attatcaact ttgttgagaa gaatacctgt aaagctaaca
     2581 cttccaacat tagacagtcg ttctctttat gaaagaaaac agttaatcaa gatatttttt
     2641 aaatcagaag aagaaagact agcaaagaaa atactgatat ctcaacaatt attagatttt
     2701 ttattaaact atcgctttag tgaaaatgtt ggcaatttga aaaatatcat tcaattgacc
     2761 tgtgccaatg ccttttattt cttagatgat ttggaagaac tttatattca gatgtatcat
     2821 ttaccacaat gggtgaattt caacggcatg aaggttatga aaagacagaa tgaattaaaa
     2881 tatattcaaa tggatgattt ggaaaaagta gacgaagttt ttaatatcaa cgaacttata
     2941 gagtatcttt atcaaacgtg gaagaaagac actgataagc agcagctttt tgagaaatat
     3001 gatagatatt ttagacaaaa gaatgttgat atccaaaata attcagcttt aacatatccc
     3061 acctatcaag agattcgatt tagacagtat gttaaggaaa tagagagaga ttttcaaata
     3121 gaatttgact attattatct tcaaataacg tctatctttt tgaattcttg gatttatcaa
     3181 atccaattaa catcggacga taaattatat tccttcatca atgatattaa tagttggtgt
     3241 agtcgagaag ttcaattagt caatcgtttt attcaaatag gggatattta tttaacaaaa
     3301 attcaaaaaa atgttttggt tattttattt aagttaatgt gtagtaaaga atttcagcaa
     3361 aaacgtttag cactgatttt ggctcatgga gatctaacgg caacctgtat agcaagtact
     3421 gctaatagga tcgttgagag atatatatac gacgccattg atatgccact aatgacttca
     3481 agtagtgaga ttgcagacaa aattaataga tacttacaag agtgtaccgg atatgaagaa
     3541 attattttac ttgttgatat gggttcactg gagaaaattt atctaaattt ggaactagac
     3601 gctcaaaaaa aattcggact actaaacaat gtttcaacaa gaatggctat tgatgttgca
     3661 aatcagatat tattagattt accgcttaaa cagattcttg aacaagtaaa agaaaatcaa
     3721 aatgtggatt atatttatga atcatcaaat attaagaagg atatgattgt ctgttcttgt
     3781 gcaagcggat taggaacttc gtttaaatta aaaaagatac ttgaagatag ctttccaagg
     3841 gatactgatc ttgtcataga aacgtgtgac tattactctt tagtgaatga aaactattta
     3901 tcggagcttc gtaaagagaa caatattctt tttattgttg gaacactaaa tccacatcta
     3961 gaagatgtag aattcttttc
//
LOCUS       DAEGIQ010000171         3946 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000171 DAEGIQ010000000
VERSION     DAEGIQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.180"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            24..1013
                     /locus_tag="KMT45_002753"
     CDS             24..1013
                     /locus_tag="KMT45_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBE7006175.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            995..1456
                     /locus_tag="KMT45_002754"
     CDS             995..1456
                     /locus_tag="KMT45_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBE7006176.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1506..1970
                     /locus_tag="KMT45_002755"
     CDS             1506..1970
                     /locus_tag="KMT45_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE7006177.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1984..2733
                     /locus_tag="KMT45_002756"
     CDS             1984..2733
                     /locus_tag="KMT45_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBE7006178.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2733..3563
                     /locus_tag="KMT45_002757"
     CDS             2733..3563
                     /locus_tag="KMT45_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE7006179.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3563..>3946
                     /locus_tag="KMT45_002758"
     CDS             3563..>3946
                     /locus_tag="KMT45_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBE7006180.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFD"
BASE COUNT         1341 a          517 c          759 g         1329 t
ORIGIN      
        1 atgactttat aatacttatt agagtgaggg ataaagaaat gagacaacta cacaaagaac
       61 agattgaaac agcaatggca gcgttaaagg aaaagacaat tgatcatgta tattttacag
      121 catgtggggg ttcacaagcc gttttaatgg ctggtcaata tctatttgat aaagaattaa
      181 ctgtaccttg tcatgtttat actgctaacg aatttaacta tgctatacca aaaggattat
      241 ctgaaaattc tttagttatc acttgttccc attctggcac aacaccagaa actgtagaag
      301 caacaaaaaa agcaagagaa tttggagcaa tgactatttg tttatctaat gaagaaggat
      361 ctcctctatg ggaagcagct gaatatcctg ttcactatga ttggggtaaa gatgttgatg
      421 ctagtgattt aaataaagga attctttatg gtttattatt taatatttta cttgttttag
      481 atggctctaa aaaatgggaa acttgcttga atgaatttga taagttaaat gatcttgtta
      541 ttgctgaaaa gagcaaattt gaaataactg ctaaagaatg gggtaaaaac caaaaacgag
      601 ataaaattat ttacacaatt ggtagcggta tcaattacgg agaaatttac tccacagcag
      661 cttgctggtt catggaaatg caatggatta attctaatgc tattcattct ggggaatttt
      721 tccatggtcc ttttgaaatc actgattatg atgtaccatt catcttagtg aaaagtattg
      781 gagatacaag atttttagat caacgggtag aagacttcac taaaaaattc agtaataaat
      841 taatggtttt agaccaagct gattttgact tagaatctat agatagtgta gcgaaagaat
      901 atattgcagc tgtacttagt ggcgttgtta ttcgccaaat ggtagaagcg attgcttttg
      961 aaagaggcca ttccttagat gttagacgct atatgtggca aatgaaatat taattaatag
     1021 gaggttgcaa aagatggcaa atcaattatt tcacattgtg attacaacac attctaccct
     1081 ttgtgagggt tatttaaagg caacaaaatt aattttaatg aatgacgcca caggtattag
     1141 tgctgttcca tttaaagaaa atactagtcc agatgatttt gaggagagta tgaaagaaat
     1201 aattagtcag catcatttgc aacctctaat tattttaacg gatttaattg gaggaacacc
     1261 taataatgtt tctattaaac agttgaacaa caatagaatt caaattattg ctgggattaa
     1321 tttacctctt ttactggagt tattaatggc ccagtcagct ggtaaatcta tagaagaaat
     1381 agattttaag gaattactga ataactctca aaaaagtatg gttcatatta attcgctttt
     1441 aaaagatgtc agttaaggtg gcaatttcta ttctataaat tttaaaaatt gaaggaggaa
     1501 atagaatgat taaattagtg cgtgtagacc acagattact tcatggacaa gtaatatttt
     1561 cttggactaa acagtatgat attaaccata ttattgtggc tgataacaaa gttccaggag
     1621 atccaatgtc tgtgatggct ttgacaattg ctaaacctgc tgattgcaaa ttagacattg
     1681 ttaaaattag tgatgttaaa gggattatgg aaacatcccc aaatgataat tacatgattt
     1741 taattaaagg acctaaggaa gcttttgaat tagttcaaaa tttaccagaa attaaagaaa
     1801 ttaattttgg cggtgtgtct aagaaaagcg agtccaaaca atatggaaag gcagtctttt
     1861 taactcctga agaattagaa gccacaaaaa aaattcttga tcaagaagtg aaaatatttg
     1921 tgcaacaagt cccatcttca agtattgaaa atgtagattt tagtaagtaa ggggggaaaa
     1981 taaatgttat taaaagccat tttattaggt ttagttggtg tggtagctgt tattgactcc
     2041 aggttaattg gtcgacaaaa tattggtaga ccactaattt tgagtacact tgccggactt
     2101 gtgctaggtg acttgcatac agggattgtt cttggggcat ctttagaatt aatttctatg
     2161 ggattcgtct caattggggc ggctggacct cctaatatgc aacttggtag tattattgcc
     2221 actgcttttg caattttaac taatagtagt acagaaacag ccttaacaat agctatccca
     2281 gtggctgtag ctggtgaatt tatgagtatt atcatgcgca tgataattgc tcaatttgga
     2341 catgctgcag ataaagccat tgaaaataat aaatttagaa aagctcaaat tattcatatt
     2401 tattggtcat ttatttttaa tgcttttgtt tactttattc caatcttttt aacagtatat
     2461 tttggtgctg acgttgtggc agatttagta gataaaattc cagaaatgat tacagacgcc
     2521 ttgactgtct ctggaaatat gctttccgca ttagggtttg caatgctatt aagcacaatg
     2581 ttgagtaaaa aattatatcc ttactttatt tttggattct ttattgttgc ctattcaggt
     2641 ctttcactaa ttggcgtgac aatttttgca gctctgattg cctttgtaat ggatcaagtt
     2701 aaatatggaa aaagagaaga ggcgcatggt taatggaaaa tagtacagaa aaaaaacgtt
     2761 taatcacaaa aaaagatctt aataagatgt gtcttcgttc atggacaatc atgtgttcgt
     2821 ggaattatga aagacaaatg catatgggat ttatgtatgg gatggcgcca attttaaata
     2881 aagtttataa aaatgatgaa gagaaaaaaa gagaagctta tactcgtcat atggcatttt
     2941 ttaactgtac gcctcaaatg acttcattta ttatgggact ttgtgcttct atggaagaac
     3001 aaaatgctca agcagaagaa ggaaacttta atactgaatc tatctccatg attaaaacca
     3061 gtttaatggg tccttttgct ggaattggtg attcattttt ccaaggtact ttaagaatta
     3121 ttacatttgg gattggattg tcttttgcta agcaaggtag tattttagga ccgattttag
     3181 ctgttggcct ttttgctata ccttctattt tagctgtttt ttacggcggt aaattaggat
     3241 atgagtctgg caataaatac cttactaaat tgtatcaaga aggtcttatg gatcgagtaa
     3301 tgcaatttgc ttctatcatt ggtttggctg tagtaggtgg aatggtggct agcatggtgc
     3361 caattgtcac ccctttaaaa ttcactacag ggggaacaga acttgtttta caagacttac
     3421 ttgattccat tattcctgga ttattaccac ttgtatgtac tttgggaata tataaattga
     3481 ttaagaaaaa tgttagtaca aatatgctct tattaggttg tattttggca gggtttgttt
     3541 taagtataat aggtatctta taatgattaa ggctctaatt tttgatatgg acggagtttt
     3601 aattgatagc gagattatgt atcaaaagtg ggtatttaat tttttaaaac aagaaaattt
     3661 aagctatcct ttgtattctt atcaaaaaaa gattggaact acagcaagta tttttgatga
     3721 tatagaaaag tacaatgagg gatgtaacag tttagaattg aagggaaaat ttactaaata
     3781 ttggaaatcg caaaagattg attacttgga tatttttcct aatatactaa ttgaacagct
     3841 tgaatggtta aaaaagaaaa aaataaaaat ggcaattgct tcttccagtc caatggatac
     3901 tataaatgaa gttgttacca cttgtaaaat agagtcatat tttgat
//
LOCUS       DAEGIQ010000172         3944 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000172 DAEGIQ010000000
VERSION     DAEGIQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.181"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1211)
                     /locus_tag="KMT45_002759"
     CDS             complement(<1..1211)
                     /locus_tag="KMT45_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBE7006181.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKM"
     gene            complement(1201..2796)
                     /locus_tag="KMT45_002760"
     CDS             complement(1201..2796)
                     /locus_tag="KMT45_002760"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBE7006182.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            2921..3706
                     /locus_tag="KMT45_002761"
     CDS             2921..3706
                     /locus_tag="KMT45_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HBE7006183.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1233 a          762 c          640 g         1309 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggtcgtgta
     1201 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
     1261 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     1321 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     1381 atcgacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     1441 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     1501 gttttcttcc gataatttat tctgattttt ccctttaaca aattcacgac tagcatcaat
     1561 gaataataca tctcgagttt gacgattctt cttcaataca atcaccgtcg ttggaatcga
     1621 tgtaccaaag aacaagttcg caggcatacc gatcacagca taaatactgc cgtcttctaa
     1681 taatttttga cgaatgaccc cttctgcagc accacggaat aaaaccccgt gtggcaagac
     1741 gatagccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa acgcaaaatc
     1801 tgctttggat tttggcgcta atttcccgta gcgattaaac cgagaatcat ctaagaaggt
     1861 cgtatctgct gaccaattag ccgaataagg tggattcatc actactgcat caaatgtgta
     1921 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     1981 ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     2041 ttcttgccca tgatatttaa cgttatctgg atgagttaag tagttacgta catttaacat
     2101 caaggaacct gaacccatgg ttgggtcaaa gacactaaag aatcgtcgtt ctttttgatc
     2161 caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa attctccggc
     2221 tttcttccct gcttctgaag caaattgact gatcagaaat tcatacgcat ccccgatcac
     2281 atccccatcg tgacctaaaa cttccacgtc attcaatttc ttgataactt cggtaatcgt
     2341 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
     2401 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
     2461 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggtga
     2521 gatcgaatac cccaaaatat caacgattgt ggcaatcagg tcttcttttg attcttcatc
     2581 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
     2641 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
     2701 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
     2761 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
     2821 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
     2881 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
     2941 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
     3001 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
     3061 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
     3121 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
     3181 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
     3241 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
     3301 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
     3361 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
     3421 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
     3481 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     3541 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     3601 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     3661 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     3721 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     3781 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     3841 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     3901 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DAEGIQ010000173         3935 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000173 DAEGIQ010000000
VERSION     DAEGIQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.182"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(7..549)
                     /locus_tag="KMT45_002762"
     CDS             complement(7..549)
                     /locus_tag="KMT45_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBE7006184.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            684..1073
                     /locus_tag="KMT45_002763"
     CDS             684..1073
                     /locus_tag="KMT45_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006185.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1077..1841
                     /locus_tag="KMT45_002764"
     CDS             1077..1841
                     /locus_tag="KMT45_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBE7006186.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1970..2917
                     /locus_tag="KMT45_002765"
     CDS             1970..2917
                     /locus_tag="KMT45_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7006187.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3047..3787
                     /locus_tag="KMT45_002766"
     CDS             3047..3787
                     /locus_tag="KMT45_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006188.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1298 a          676 c          804 g         1157 t
ORIGIN      
        1 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
       61 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
      121 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
      181 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
      241 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
      301 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
      361 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
      421 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
      481 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
      541 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
      601 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
      661 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
      721 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
      781 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
      841 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
      901 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
      961 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
     1021 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
     1081 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
     1141 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
     1201 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
     1261 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
     1321 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
     1381 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
     1441 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
     1501 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
     1561 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
     1621 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
     1681 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
     1741 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
     1801 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
     1861 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
     1921 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
     1981 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
     2041 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
     2101 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
     2161 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
     2221 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
     2281 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
     2341 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
     2401 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
     2461 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
     2521 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
     2581 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
     2641 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
     2701 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
     2761 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
     2821 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
     2881 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
     2941 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
     3001 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
     3061 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
     3121 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
     3181 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
     3241 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
     3301 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
     3361 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
     3421 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
     3481 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
     3541 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
     3601 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
     3661 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
     3721 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
     3781 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
     3841 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
     3901 gcgaattttt tctcccaata agtaagaaaa taatg
//
LOCUS       DAEGIQ010000174         3907 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000174 DAEGIQ010000000
VERSION     DAEGIQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.183"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            9..689
                     /locus_tag="KMT45_002767"
     CDS             9..689
                     /locus_tag="KMT45_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBE7006189.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIPA"
     gene            1393..1488
                     /locus_tag="KMT45_002768"
     CDS             1393..1488
                     /locus_tag="KMT45_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBE7006190.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1790..2035)
                     /locus_tag="KMT45_002769"
     CDS             complement(1790..2035)
                     /locus_tag="KMT45_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006191.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            2380..2664
                     /locus_tag="KMT45_002770"
     CDS             2380..2664
                     /locus_tag="KMT45_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006192.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2667..3083
                     /locus_tag="KMT45_002771"
     CDS             2667..3083
                     /locus_tag="KMT45_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006193.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(3220..3897)
                     /locus_tag="KMT45_002772"
     CDS             complement(3220..3897)
                     /locus_tag="KMT45_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBE7006194.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT         1311 a          723 c          638 g         1235 t
ORIGIN      
        1 gaatgacgat gaatcatttt aaaggaaagc aatttcagca ggatgtgatt attgtagccg
       61 tgggctacta tcttcgttat aaccttagct atcgtgaagt tcaagaaatc ttatatgatc
      121 gtggcattaa cgtttctcat acgacgattt atcgttgggt gcaagaatat ggcaaactac
      181 tctatcaaat ttggaaaaag aaaaataaaa aatcctttta ttcatggaaa atggatgaaa
      241 cgtacatcaa aattaaagga aaatggcatt atttgtatcg agccatcgat gcagatggtt
      301 taaccttgga tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta
      361 agcggttagt gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta
      421 ttacaagtgc ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta
      481 ccattaaata cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata
      541 aattctatcg aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag
      601 gattatataa gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa
      661 tcaaggtatt attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattcttt
      721 atttaaaact ttgcaacaga accattccgt tttttcatcc attcctttct tcccccctta
      781 caaataaata ataaccttta acaaccaact cgtaaatagg ttggttgtta ttatacaaaa
      841 ggatattagt actcagataa aaagttgtta gtactcagat aaaaagttgt tagtactcag
      901 ataaaaagtt gttagtaccc ttctgtatcc gttgggagag taaggctcaa ctgcccccta
      961 aaaagggttc taaaaaatct ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa
     1021 tttcccgcca aaaaatcacc cactttttaa aataaacagg ttcgcgcatg atattaaact
     1081 gccaatctag ctccctatgg tcgctgtaag aaggagtgat cgctacgctc tcacatttta
     1141 cttgcagcag tcgcctttag gctccttcgc caaaaataat tcattaggga ctcctttgtg
     1201 gcgtcctgag agctcgtcta agccgttcta agacaagatt cgataaaaac tatgtccttc
     1261 gttctaaagc gccttacagg cggtcagggc aagccctaac aaccccaagg caataaaaaa
     1321 cttaggctta tttttgcttt tttattgtat aattgattgt cgcctatccg acagtcttgc
     1381 gactggaagg aggtgtgttt cgtgttcact aactttattt gctcccttct tgttgggctt
     1441 ttaagcgctt tgttcagtta ctggttgaat aaccgaaaca agaagtaaaa cttaattaag
     1501 gcgactcagc ccacaaaaaa agccccagag aatccgacct ctctggggct ttcgtgtgtt
     1561 tcgtgttcac ttatttgcaa tcaaattata acagaaataa atatttattt caaaaatatc
     1621 ctacaaaagt ctaaaatccg taattatttc ttatatgcaa catttacaaa taaagtcgct
     1681 ctgagagctc gtatttttcg atttaagacg ttttatttag tttttggtgt atttcattgg
     1741 tccaagctaa aaaactctta gaagcgattt tagagccatt taaacccact taattatatt
     1801 ctccatgaaa ttcgctccat gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca
     1861 ctagttgcat taatttttcc aacgatcgtt ttctttcggg tgtcatattt ttatattcca
     1921 acaatttttc gtctgttaaa tcgaacttga ttgcttgaat tgcttgctct aatttttcta
     1981 agttttgaaa atattcgtaa ggaaattttt ccattttttc tttctccagt ctcacaattt
     2041 ttctagaaat aaaatcactt ctttgccgtc tatactctca aaaaaaatgt cgtttgaaaa
     2101 actaaataaa tttttgatcg tgcattttta aatttggtta tcgattacac gtgcagcact
     2161 cccccccgaa ttttaaagag cataaggaaa cgtgtaccca ctttctaaaa aaagtgggta
     2221 gataggctgt gcctatcacg tttctcagaa aaaaatttga tatcactttt tcgccatcgt
     2281 gggtagctta tgtatagcat tttggcaaaa aacagctata caaatatagt atttgtatgg
     2341 tatacataaa ttatacataa ttgcaaaagg agatttttta tggacatttt agttcggaat
     2401 attgatccaa agtttgttgc aacaatagac aaacgttgcc aagaactcac gaataaaaca
     2461 ggagaaaaat ggagtcgaaa taaatatcta aaagttttaa tcgaaagtga ttttgaccgt
     2521 caacttacga cttacaaaaa agaccaattc gatttattgc tcgataaatt tattgctatc
     2581 caaacagaaa atactcaaat attaagcgag tatgtctaca caaataatca aatcattgaa
     2641 atgcttatcg gaggaatgat ataaccatga gaaaaacttc tgtccaaaaa catgttactt
     2701 tgtaccctag tcatgttgac gttttagatt ccatcatcga aaaaaatcca gatatcaata
     2761 actactcgga agccattcgc tttttatgta tgagttatga acccgatacc aacacgacca
     2821 ctaccagcaa tcaggcaaaa ctaaatgcta tgggaaaaga aatttccatc atgactgaaa
     2881 tcatcgcaga atttgctgat ttatatctaa aagatacagg cgttcttatt ggcacaaatt
     2941 ctaaagtata tcaagaagtg aaagaaaaaa tcgaagctaa actaaatcca tcaaatcgaa
     3001 atcgaccaaa gaatcatcaa aaagaaacca gtcaaacagt cacttctaga aatttcgacc
     3061 caacaaattt tctaaaacga taaaaaacag ataggagtta ttcctatctg ttttttattt
     3121 agaagcccca tacaatatgc tagaatgtac tagcaatctt agtcggttgt aagggacttg
     3181 tctacttgct taatgtaagg ggggcgagtt cttttttatt tattcttcta accagttacg
     3241 cagagaaatc tttggcaaat ctgacgttag gcttgaaaaa gtaaattcta atttggcgat
     3301 tttattgccc ttttttgctt ttatttttgt gactttaaga tcattgaagt atttttttag
     3361 ttctcgtaat gcaggattta atactttttg atcaatgtta cccatttgat agtattctgg
     3421 gatatccatt aagctcctaa actcttcaat acgaactaca taataaccag tagaacggta
     3481 ctgcataagc agtcggaata gagtttttgc atacgtagaa cgcaacgacg tgaaagcaga
     3541 taattcaaat ctactaaatt cagctgttaa accattaata atatattcta ggtccggatt
     3601 aacactgact tcaacaaact tttttgtttt attgatttca aaaccagtaa acaaaacaaa
     3661 gtagcgaata gaatcctcat cttcagtacg gtaagtaaga ttaagcatat ctttatagag
     3721 cttttctaaa tcaccgacaa aggcttttgt agcagtcatc ttataatcac ttaactcttt
     3781 taattcctcg aaagaaaatc taactttttt caaaccacga tctttcatac gagcgcataa
     3841 tgcaaaaaat aaatccatct gcttagcatt gaaatttttc agcggtacta aattcaattc
     3901 attttga
//
LOCUS       DAEGIQ010000175         3878 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000175 DAEGIQ010000000
VERSION     DAEGIQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.184"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..305
                     /locus_tag="KMT45_002773"
     CDS             <1..305
                     /locus_tag="KMT45_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HBE7006195.1"
                     /translation="GAMSPTEIFKMFEMGADIVKVFPANCLGPEYFNQVQAPLGSLPL
                     MAVGGVDQTNAQNYLNNGASYVGIGSKFFEKSAVHQLNYERLMELAESFIDSLRVE"
     gene            complement(699..1706)
                     /locus_tag="KMT45_002774"
     CDS             complement(699..1706)
                     /locus_tag="KMT45_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE7006196.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2173..3579
                     /gene="lacG"
                     /locus_tag="KMT45_002775"
     CDS             2173..3579
                     /gene="lacG"
                     /locus_tag="KMT45_002775"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBE7006197.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3602..>3878
                     /locus_tag="KMT45_002776"
     CDS             3602..>3878
                     /locus_tag="KMT45_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBE7006198.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLREL"
BASE COUNT         1278 a          576 c          757 g         1267 t
ORIGIN      
        1 ctggggccat gtcgcccaca gagatattca aaatgtttga gatgggtgct gatattgtta
       61 aggtgttccc cgccaactgt cttggaccgg aatatttcaa tcaagtgcaa gcccctttag
      121 gaagtttacc tttgatggct gtaggagggg tagatcagac taatgcacaa aactatttga
      181 acaatggagc ctcttatgta ggaattggtt caaaattttt tgaaaaaagt gctgttcacc
      241 aactaaatta cgagaggcta atggagttgg ctgaatcttt tatagatagt ttaagagttg
      301 aatagaaccc atttgcggaa tacaacttgt tttataatat ggcgttatct gtataaaaca
      361 tagtatcaca gtgaatgaga ttgaactggt agattgattt ttcgaccagt tctttttgcg
      421 cgctctgatg cttgatatat tcccaacgag ataaaaaatc ccccaagata ctaagatgaa
      481 gcgtttatcc aacctcatac ttagataaaa aggaggaaat gttctgaatg ataaacaaaa
      541 attagcgcgc acaatttggc gatgaaaaaa tcatattgta tttacgtcaa aatatcacca
      601 tcaaattatt tatggaaaac ataataggca taaacccgat tgctcggcta ctatgtggac
      661 acaattatag ttttaaagat aagtttcaat tacttatttt acacctcttc tttagggata
      721 aaacttttcc taacggtcaa ttttgcgccg attaatgttt tctttgatat gtcccgcgaa
      781 tataccagtt gatcacttaa taaatctaca caggttttag ctatctcttc gatatctatc
      841 ttaaaagttg taagtggtgg tgcaacgaat tttgcgatgt cgtcatcatt aatacttatt
      901 actgccatat ctttagggat gttaataccc aattcatggc agccctgaag aacgccggca
      961 gctatagtgt cagatggtat gaaacatgcc tcgggcatat cttcatttaa ctcagctttc
     1021 attttaagag ttaattgata cccttcgtca acagtaaatg atccttggga gaaaatatac
     1081 ttttcattga gtagtccgct cttttctaaa tgatatcgga aggcatattc acggctatca
     1141 atctcttctt tacccgtttc taggttaaag aaatttcctc caataaatcc aatttttgtg
     1201 aaaccttcat taataaagta gttaattgct tgtctggcca tttcgtggaa atcaggttct
     1261 acagtatcga agtgttcagg taatgggttt attcctcgga ttaccccctt ataccctttt
     1321 tgatttaagt agtacaattc cttttttgtc aattgtccta ttgcgataaa tccagtaata
     1381 tcgtcaggaa tgctttttac accatcgttt ttagagatga aaatcaaatc tagattagct
     1441 tttttactat aatttttcaa atcatctttg acatgttgat agtagacatc cttttttaac
     1501 tgttcctctg agatccaaaa aagaatggca atcttgtaca gagtagtctt aatttttgtc
     1561 agatcatatc ctaaagattt ggctgtaacg attattttgt ttttagtatc agatgtaata
     1621 gagagtgact catcaccttt aaaaagtctt gatatagtag ccgttgagta accagatttt
     1681 tttgcaatat ctctaatagt aaccattgtt catcctccta gggatataat aatccttttc
     1741 tttgttaaag taaatcgaaa ctgctctttt agtaacttaa attacttttt ctagcaggaa
     1801 acgaaactct gatatcagca gagagcctaa tccattaaca gagaagattg aatcgataat
     1861 ggatagccac ttggagaatg attagtaggt gggattgatt ttagtgggaa tcagtccgtt
     1921 ttcttgtttc gtgtaaatta aattagtaaa aagataaatt tattttacaa aaaaacgttt
     1981 acaaaacggt ttttgaatgg tatatttatt ttacatcaag ggatataagt gcttgtatat
     2041 cgcaataaaa tagatattcg aggtcggttt ttcgttgtgt aaaaaaattt acttatacat
     2101 gttaaaatta tgctgaggtg atttatgggt tattggcata ttgatcaaac taattttcaa
     2161 ggagagatta aaatgaatta taaaacttta ccagaagact ttattatggg aggagctacg
     2221 gcagcttatc aagcagaagg ctctacaaaa gaaggtggaa aagggcaagt agcgtgggat
     2281 actttccttg tagaaaaggg acgctatact gctgatccag ctagtgattt ttataatcaa
     2341 tatccagttg atgctgaatt atgcaaaaaa tttaaaattg atagtattag gatgtctatt
     2401 gcatggagcc gtatttttcc aaaaggctat ggggaagtga accaaacagg agtggaatat
     2461 tatcataagg tatttgctga attgaataag catggtatta ttccttttgt aactcttcat
     2521 cattttgata cccctaaaga actatttgat aatggagatt ttttaaatcg agaaaatgtt
     2581 gaacatttcg taaactatgc acagttctgt ttcgatgaat tttctgaggt tcattattgg
     2641 agtacattta atgagattta tccagttgct tcaaatcaat atttgatcgg atcgtttcct
     2701 ccagaagttc aatttgatat agagaagact gtaacttgtt tacacaacat gatgtatgcg
     2761 cacgcaaaga cagtaaacat ttttaaagat ggtggatata aaggggaaat tggtgttgtt
     2821 cactcgctag agacaaaata tccagcaagt gacaaagaag aagataagca tgctgcgaaa
     2881 ttagatgatg cgttatcaat taagttctta ttagatgcta cttatttagg gtattactca
     2941 gatgaaacaa tggcacatat taatgagatt ttagcagcaa atgggcaatc tgcagatatt
     3001 gattctaatg attttagaga gatgaaaaaa gcagcaaatc gaaatgattt tcttggtatc
     3061 aataactatg gtagccattg ggttaaagag tacaaaggag aaagcacact tcactacaac
     3121 actaccggag aaaaaggtag ccaagtctat tgcattaaag ggattggcga gagaatcgtg
     3181 cgtgaggatc ttccgaaaac cgattgggat tggatcattt atccaaaagg gttatatgat
     3241 ttacttgtac gaatccaaga cgaatatcca aattataata aaatatacat tactgaaaac
     3301 gggctagggt ataaggataa ttttgaagat gggattattc tagatgatga tcgaatcgat
     3361 tatttacgcc agcattttaa tgcgatttcc gatgctatca aaatgggagt taatgtaaag
     3421 gggtatttca tttggtcatt aatggatgtt ttctcttggg ttaatggata cactaagcgt
     3481 tatggactat tctatgtaga tttcgataca caaaaaagat atccgaaaga gtctgcatat
     3541 tggtataaat ttgttagcga aacaaaaacg ataatataaa aatagttagg agggcaagga
     3601 aatggatagg aaagaattgc aattattagg atttgaaatt gttgcttatg caggagatgc
     3661 cagaagttca ttattaacag tgttgaagga agtgagagaa ggtaattttg ataatgttga
     3721 acgattatta gcggatgctg atgaaaattt aaccttagcg cataactcac aaacgaaatg
     3781 tttagctgaa gaagcgtcag ggaacgaaat ggaagtaggc gtaattttta ttcatgggca
     3841 agaccattta atgacaacga ttctattaag agaactta
//
LOCUS       DAEGIQ010000176         3764 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000176 DAEGIQ010000000
VERSION     DAEGIQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.185"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            158..976
                     /locus_tag="KMT45_002777"
     CDS             158..976
                     /locus_tag="KMT45_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBE7006199.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            980..1276
                     /locus_tag="KMT45_002778"
     CDS             980..1276
                     /locus_tag="KMT45_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006200.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(1458..1811)
                     /locus_tag="KMT45_002779"
     CDS             complement(1458..1811)
                     /locus_tag="KMT45_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006201.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(1831..2886)
                     /locus_tag="KMT45_002780"
     CDS             complement(1831..2886)
                     /locus_tag="KMT45_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBE7006202.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            3102..3500
                     /locus_tag="KMT45_002781"
     CDS             3102..3500
                     /locus_tag="KMT45_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006203.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            3503..3676
                     /locus_tag="KMT45_002782"
     CDS             3503..3676
                     /locus_tag="KMT45_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006204.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         1267 a          546 c          681 g         1270 t
ORIGIN      
        1 aatatataca taaacataaa catatacata tatatgttta tgttgtagaa tataaaagca
       61 aatataaata taaacacgtt tatgtatata aacatataca taaacgtgtt tatagtgtat
      121 aatggagtta caaaaggtta ataagggagg tttaatcatg gaaaagaagt tgtcaatcat
      181 tactggaaac attgaaaaag gtggaacagg taaaacaaca acaatctaca atctaggtgg
      241 atatgctgtc gaaaatttag gtatgaaggt gctgtatatt gatgaagata agtcgcaaaa
      301 cctatcaaaa cgatttcctg atgctttcag taaagcgaaa agagagaata caatcaatca
      361 tttatatctt catggatcag ctgaaccttt agctgtaaat gagaacatgg atattttagt
      421 agcaggtgaa gatttaaaac aagttgagat gcaaatgcgt gatatgccta acaacagaac
      481 cctactgttt agatggatgg ttgttaacta tgaacagctt aaagagaagt atgatctaat
      541 tctgattgat actcataatg atgcaggtct actagctcaa aatgcttggg ctgtatctga
      601 tcttgtcgta ggcatttctg atccatctaa agatggtttt gaagctttgc tgaagcttgg
      661 taaagatatt gatgcactag caaaacagtt gattgatatt aagactggtg aaacactgat
      721 ggaaactaaa tacgctacga tcggtagcag gatcaaacat aatactaatt caagtcgtga
      781 gttcctagaa ctggtaccaa cgttatctaa ttatcttgga tatgtacaag agaaggaatt
      841 gcttaatgca actagcttag aaggtaagaa tattgctgag ttcgctaagg ataagaatat
      901 ctatcgtaag aataaggatt tctttgaaag cacgtttacg ctatacaaca aaataattga
      961 tacggcacaa gaataaggag tgattgaagt gaaagaaaat agatttaaaa aattcgctga
     1021 agctgaagta gaagaagttc ctgaagtggt tgaagcagtt gaagaaccta aacaaacacc
     1081 taaagaaaca acaagttctt tggtagctaa actaaaaagt gatagttctg aaaagaagaa
     1141 aaattatcaa ttcacgcttc aaccatcgca aagaaagaaa ctatctaaaa tcagcgaaaa
     1201 gttaggaatt cgatcagatt cagctttact tggtgaaatg atagataact tgtatgatga
     1261 agtttttggt gagtgatcag tttaaagctg atcacttttt ttattggaat aagcggttag
     1321 gtatagctta actaaattta atatacgtgt ttatggatat atacgtatac atgtttatat
     1381 acatgtatac gtatatgttt atgtttatat ttgtacataa taaaaaggct aggtattgta
     1441 acctcgcctt tttattttta aagtaagtct ttgttacttg gtgcattgaa ccctttcagc
     1501 attggctttt ctttctttgc tgaatgatcg gatgtaaccg ttgaaggatg ctcttgttga
     1561 atagggttat gatcagttgg tttattcttt acttctgcta ctttgctatc gtttctatcg
     1621 ttgcctgctt caataattgt aacttcattg ttctttacac gccgatatag gttcaaagct
     1681 tttttgatcg tctgagagta tttaacgcct agtgtttcat attcggattc aagaaactct
     1741 gctaaaggta actcgctaac cttttctcta ttgattataa ctacataacg ctctgatttt
     1801 cccttattca tgttgatctc cttcgctgtt ttattgagtt aaaccaaact tgtaaaagcc
     1861 ttttacatta gaaaattcac tgtcttcaac aaacaaggta tgcggaattt tatcttttac
     1921 aagttgtttg tcgataatag aagctccacc accagtaaag acaactttat ctaaggttgt
     1981 gaagtttttg aatgtagcta aaacttctgc aacaaccata ttggtgtatg tttttatttc
     2041 tctttcaact acttctgaaa tatcaaaact ttcatttccg cttggcttaa atagatatac
     2101 gcctttttct ttgccttctc ggataatctt ttcaagttgg aaagcattag gactgatcgg
     2161 aaggtcaagc atatttacga tacgattata taaagtaaaa atgccggtat ccttttgttc
     2221 acgattgttt tcgtcaattt caaaattttg gacaacatca aaaagtttcg tgccaccgcc
     2281 aatatcaaca aaaccgatct tctgactgat caatgtttca tcttgtacac taccatcatc
     2341 agcaagtagg aggttatata gtgttccaat cggctgtggc agtacccaaa cggtatctac
     2401 gtttatatta taagctttac cgtcaacttc aactgaatgt tgtcgtgtgg cttgttgcat
     2461 cacgtatttg acgattgttt cggtatagtc ggatgaaggc agacctaaaa ctagattagc
     2521 ggttaaaacg tcatcagatt gatcttctaa acgtgctagg ttagctaaag agaaagatag
     2581 gatattttgg taggtttcct gcttgtatct gctgatccca aaacctagtg attctatcac
     2641 gtagtcatct tttcctagct cgtgtatatc cttcccaaag taatagcttt cagtagtgtt
     2701 tactaggcta tattcagaaa cgtttagtga tcgttttaat gaggaaccta tattagaaaa
     2761 acgtcctgaa ttaatgatat ttttgttcaa aatacgagag ggaagtgaga gtggagaaac
     2821 gtgtgtactc atttctttta ctgatttatt ccctaagtca acggaaaaca ataatggttc
     2881 tttcatggta tgacctcttt ctgtttgtgt ttatggtatt tcggtacgaa aatagtttac
     2941 acctatttac tattagttgc aactgtttat aagaaaaaaa tgcaatcata aaagtctaat
     3001 taattataat aaagtgtaaa taaaaaggtg taaataaatg cttgcaaatt tatcttatta
     3061 tgatattata aaaacataaa ttatattaag gagtgatcct gatgcgattg agaagaaata
     3121 agaattacac taccattatt ttagaagatt ttactgatca agaattactt attgtcggta
     3181 aaacaaaagc agattttatc gaaaagggaa atgcttcaat agtaaaagct tcaaagattg
     3241 ctgtcgaaaa atattttgat tctttagaag aaagtgctaa ggaaattttt aatggagaga
     3301 aagctggtag ctatgagtta gacggtatta gatttagctt gatcccaaca actgaaaata
     3361 ttattccgaa gaaattatat tatatctttt catctgaaaa acatacttca atgaagcctt
     3421 gtatttttga ttggcaatgg ttgattgatg ctgtattgga tgtaaaatgt tcaaaataca
     3481 atattacttt ggggtgttaa ttatgagtat gctagttaga aaattaccat tatctgtaag
     3541 agaatttcta ttttacttgg tctgcttaat cgcatcaggg tttaagctga tcgaacatat
     3601 ttttatcttt gcttatgtat tagcaatgat cgcattttta tctggcggtt ggttaattat
     3661 ttatttgtta agctaagttt tagatcaaaa ataattaata aattaaacaa taaaataacc
     3721 gttaataaat gtaatgaaag gacaacgcct aacaatcagc ttgt
//
LOCUS       DAEGIQ010000177         3747 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000177 DAEGIQ010000000
VERSION     DAEGIQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.186"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            58..510
                     /locus_tag="KMT45_002783"
     CDS             58..510
                     /locus_tag="KMT45_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006205.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="KMT45_002784"
     CDS             524..1213
                     /locus_tag="KMT45_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006206.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..3689
                     /locus_tag="KMT45_002785"
     CDS             1338..3689
                     /locus_tag="KMT45_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBE7006207.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1278 a          487 c          774 g         1208 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     3661 ttcaagaaaa tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt
     3721 cctttttaaa tatatacgat aaaataa
//
LOCUS       DAEGIQ010000178         3728 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000178 DAEGIQ010000000
VERSION     DAEGIQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.187"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(514..738)
                     /locus_tag="KMT45_002786"
     CDS             complement(514..738)
                     /locus_tag="KMT45_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006208.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            929..3706
                     /locus_tag="KMT45_002787"
     CDS             929..3706
                     /locus_tag="KMT45_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBE7006209.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFKQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT         1289 a          631 c          714 g         1094 t
ORIGIN      
        1 atagatgaaa ctctatttac ttctttttta atttgttctt gcaatctttt ttgctacgta
       61 atttttgtaa aaaagtgggt tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa
      121 atgagaaaaa tatttattcg tttattgtgg gtactatctt agaggaataa ataatagtgg
      181 agaaaactat ggtttggcgc agattctatc aggtttgatg gaacgaagac aacctcccga
      241 catcaagtca aaagctactt gagattttga tctgaaaata aaaaaagaaa acaaggtagg
      301 aacatcaatg tttctacctt gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg
      361 taatcaataa aaaggatggt gacaacagta cctctttact ctctgaaagc atcataactc
      421 gtttttcctc attatgccaa taaattgttg ttataccaca tataaggatt tgtaatagct
      481 aaaaacagac aggaaagttg aaatctgtga atcttatttt gtatggaagg atttatagaa
      541 cgcaaaaatc aagataaaga tgaataaaaa tcctgtttcg gctatatatt gcgtatctac
      601 gccaataaaa cgagtttgtt ctgtcatagc catataaagc aaaggcaaaa atacaactac
      661 agcgagataa gatccgatga tttctcgaaa agttttccag gaagtatgtt tcctgttttt
      721 atatttatcc gatttcattg aatagtcata gcctccttat tctttcatat gttgaaattc
      781 cagtataaaa actggaagga actgtacgtt ttttagaata aaaattataa gggataagta
      841 ggctgctgtc tataataaga tatatttttc aattctttat taaagatttt attgacaaaa
      901 tgaatccgtt ttctatgatg gaggaggggt gaataagttg ttgaagaagg aaattttatg
      961 ttacttagag aaccagacag cctttttcga tttagaacat atgaacgagg tctttacagc
     1021 acaaaggtta gctgatcatt ttggggtaaa aagaaacaca gtcagtcatt atttgaacca
     1081 gttaaatgat gagcaactac tagtcaaaat caattctcga ccagtgattt atttccacaa
     1141 agaagctttt gaaaaacaat tttttaagtt acgcacgaat ttttattttt ctatcgatga
     1201 tctcaaagag gaacagcctt ttttcgcgca accaaaagac ttattctctt ttatgatcgg
     1261 atacgacgcc agtttgaaag aaagtatcga acaaatcaaa acagctcttt actatccaga
     1321 tggcggatta ccacttttga ttacaggcga aagtggcaca ggtaaaagtt atttagttaa
     1381 tttggtctat cagtattgtc tgcttcatga tctgctagaa gattctgcac cattcatcac
     1441 ttttaattgt gcacaatatg cagataatcc tgaacttttg actagcaatt tgtttggaca
     1501 tgttaaggga gcgtacactg gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga
     1561 tggtgggatc ttgtttttag acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact
     1621 gttcacctat cttgatcaag gtgtgattta tcgtatggga gaacctaatt tgtcacgccg
     1681 gatcaaaaca cgtctctttt ttgctacgac tgaagagatt actagtcatt tcttaacaac
     1741 tttcattcga agaataccta tccgaatcga actacctgca cttaatcagc gtagtcgtaa
     1801 tgaacggcta gaactgattt actctttttt tctcgaagaa caaagaaaaa tggggagccc
     1861 tttgtcagtc agtggaccag ctttgtcact tttaacaagt cagaaattat ccggaaacat
     1921 aggagagctg aaaaatatcg tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga
     1981 acagcctgaa atccatgtca cgatccatca tttgcaaaaa gaactacttg cgcagcctat
     2041 tactaaaaat acagcacagc aaggaatcta tattacgcaa gaccagacct tagagcaatt
     2101 aatggagaag caacagcccg aacagcagcg aatcgttaaa agttttgagc aaatcctaat
     2161 tgatttcaag aaaaataact gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt
     2221 gattcaatta tttgattttt tgctgtttga gaccagtaga caagaaaaac acgagctgct
     2281 ggtttattgg acacaatcta tccgagagac ctttcgacaa atggaaactg cctatcaaat
     2341 caaattcgat ggaaacagtg tctatgcctt gagttactat ctctatcagc gaagcactat
     2401 caaatggtta ccggaagaac atgatattat ccaactaatc aaagaattag aacaacaagt
     2461 ttctcagtct tatccatcta gttatcacta tgtccaacga attctggaat taagtaagat
     2521 caaattagat attgaagaca caagtatgga tcggatttta ctgacgatct atctaaaaaa
     2581 agcgaaatgg tcaaaagaaa aacgactgcc aaaagcgatg attgctgctc acggttatgc
     2641 aactgctagc agtatggcaa atgtagtaaa tcgtttatta caagaggatc tatttgaatc
     2701 atttgatatg ccactagatg tcacaccaca gcagattgct tcagaaatcc ttgattattg
     2761 cgaaaacagc gatgtttcaa atggattgat catattagta gacatgggat ctctgaaaga
     2821 aatccatcag ttttttaaaa aacagttatc cgtaccgctt ttgattttga ataatgtgac
     2881 gacaccttta gcaatcacag taggggaatg cctgcaaaaa aatgtatcat tagaagagat
     2941 agcagaagag actgttcgac agatccagcc agaatggcga ctgctctatc cagaggaaaa
     3001 caaacccaaa gcactcatta ctacttgctt cactggaatc ggtacggcgg tccacttaag
     3061 tgaactgcta gaaaaaagct tacctacggc ttgtcagttg aaaatcatcc catatgaata
     3121 tcagcaatta aaagataaga aaaacagtga cccattattt tccatatacg aagtcgtagg
     3181 aatgattggc accacagatc ctgatatcac caatatcgac tatctttcat tagaagacct
     3241 catctcgggg gaaaagatgt ctgtactagc tgaatggttg gacactacta tgaatgcttc
     3301 agagaaggaa atattcaagc agcgcatcat tcgtaatttt tcattggaaa aagtactgga
     3361 ttccgtgact attctagata cagagaaggt gatgggagaa atcgatcatt ttatgagaga
     3421 attagaggtg cagttgaatc gaagcatcag caatcccaaa aaattagcgt tgtatgtcca
     3481 tgtgagctgt ctcattgaac gattgatccg acaaatgcca atcgaaacct atcaaggatt
     3541 agagaaatgg aagcagtgtc aaaaagaagg gttatctgct attaaatctg cgtttagtgt
     3601 cattgaagag cattatagtg tcatgatccc caattctgag attgcctata tctatgatat
     3661 tttgagtgaa gatacagagt ttctctcgat agaagaagaa ttttgaacat aagcaagttg
     3721 ttgccact
//
LOCUS       DAEGIQ010000179         3722 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000179 DAEGIQ010000000
VERSION     DAEGIQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.188"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..397)
                     /locus_tag="KMT45_002788"
     CDS             complement(<1..397)
                     /locus_tag="KMT45_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBE7006210.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQ"
     gene            complement(623..1363)
                     /gene="istB"
                     /locus_tag="KMT45_002789"
     CDS             complement(623..1363)
                     /gene="istB"
                     /locus_tag="KMT45_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBE7006211.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFPKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            complement(1353..2591)
                     /gene="istA"
                     /locus_tag="KMT45_002790"
     CDS             complement(1353..2591)
                     /gene="istA"
                     /locus_tag="KMT45_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBE7006212.1"
                     /translation="MRKGIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQD
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEAMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELLRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDEI
                     RAYTRESLKQYDLVEVADEE"
     gene            complement(2711..>3722)
                     /locus_tag="KMT45_002791"
     CDS             complement(2711..>3722)
                     /locus_tag="KMT45_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HBE7006213.1"
                     /translation="EDFKKTSISNLLVQIVTFVLIVFLIKNEGDALKYTLIQALGLVC
                     SQISVWLFIPKYIHFVKVSIRNSFNHLKGSFEYFIPQIAIMLYTNLNKTLLGFTMGSM
                     AVGYFTNSLQLNTVFITIITTLDLVLLPHMTGFFAKENVTRIVRMMEKTIHLQLYFSI
                     PIMFGMLAVYDKLVPWFFGEKFIYINNVIPYFSALIVIIPLGMSISRQYLMPIGHVKE
                     YNKSVIVGAIINIVANLILLPTIGFFGVVFSNILAECFVTFVRTRSFLKSTDFKFEYR
                     KIGIFLLGGILMCIVTRSLTSNLSSSILTNLIQFAIALPIYLTITTLFKANPFLDLLR
                     KK"
BASE COUNT         1125 a          711 c          682 g         1204 t
ORIGIN      
        1 tttgtggagt atagtgatga ccgtacattt tttcgatcaa atcagcaatt tcagacatcg
       61 taacaccttt ttcgaataaa tggataatag tggtttccaa tgtatcgttt gttcttttgt
      121 aggctggtaa agtttgttgt ttaaactcac cattacgatc tctaggtatt tccaatgtta
      181 attcaccata ttcggttttg attgatcgaa agtaagaacc gtttctcgaa ttacctgaat
      241 taaaaccagt gcgatcatat ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga
      301 gtgtgtttat cgctttttct aagtgcgaac ggaataattc atttaaatcg cctttagtga
      361 ctagagtttg cacaatttct gtagtaaaat cattcatagg gaagtcctct tttctgtgaa
      421 ttggttgtcg ttaactttat tctacagaag ggacttcctt ttttgtatgg attttttcat
      481 ttacacaaaa tattttatac tctcatctaa ataccaatac acaaagtaag atgaactgtg
      541 tcaagggcag cttaaaattg taggttttgg gcaggataaa aatgtaggtt ttcggcaatg
      601 gtaattgtcg acaatcgaca ctttagatct tgtctctcat acgataagac ttgcctgtga
      661 tcttgaacac cttgacgtga tgtactaaac ggtcaaggat ggcagctgcg atctcagaac
      721 tttggaacaa ctccccccaa cgagataaga caatgttcgt cgtaatgatc gtagagcggt
      781 gttcatagcg catggcaaga agctgaaaga ataaatcagc ttcctgtttt tgaatcggaa
      841 gatagccaat ctcatcaata atcaagacgt catagcgagc gtatcgtttg agaacacgct
      901 ctagggtacc tttttcatag gctgttcgca gacgcaaaag cagctcatga caggtgatga
      961 atagtgtccg atagccttga cgacaggctt cgattcccaa tgcaatcgtc agatgtgtct
     1021 tgcctacacc aggactgccg ataaaaacca agttctcatg tttttccaca aagcccatgt
     1081 gccttaagtc tagcacttcg cgcttattga tacttggctg gaaggcaaaa tcaaactctt
     1141 ccaggctggt tctcttggga aagcgggcgc gtgccaccac acgattcatt ttttgcttat
     1201 tttgatgatc aacttctctg cgtgtcagct cgagaagagc ttccgttaaa gaaagctcct
     1261 ctcgattggc cacttcgatg tagttaggaa catattcccg catatgattc aaaccaagag
     1321 tctctaagtt ggtcatcagt tcttggtaat ggctattctt catcagcaac ctccaccaga
     1381 tcatattgct tgagactttc acgagtatac gctctgattt catcgtcttc tttgtgactc
     1441 atgacatcgg atttcaagat ttggaaatag tcttcctcgt ggtagttcag ctgttttgtc
     1501 gttaatggat gcgaccgaag cagttctccg ttataataga tttgtacttg gtcttcttta
     1561 tcggagagtt cgatttcgac ttctttaccg atatagcgag gcgccacaga gtacctgcat
     1621 ttacggaaaa tgaccattgc ttccttggtg actttacggc gaataaagtc ttcaaaatag
     1681 ggattcaaaa ggtttttttg gtatggatgc aggtgctctt tttcgttcgt ctgccaacgt
     1741 tcatctggtg tttgatcagt tgattgagaa acctcgttat ttaactcaac acaaaggtta
     1801 ttcactaaat gacacagctc aacggcatca taaaattcat gattatagac ccgtaggcgg
     1861 tcaacagttc tggctaaagc ttcgacacag ccctttgttt gcggacgaaa tggccgacag
     1921 gcaattggtg tgaagccggc atccttactg aaagcataaa aacgtttgtt aaaatgtggt
     1981 ttggaaaact gactacgaga atggtcaacg actgtcttca tattatcgaa ccagatttct
     2041 ttgggaacgc caccggtttg ataaaacgca tcatctaagc actcaaagag tgtgtcttgt
     2101 tttcggtcga aagttagtgt gacatatttt ttcttggaat atggcaagac gtacaaaaag
     2161 atattgaact caaaaatttc gccctgatta ctgattagtc gcatatcttc cttccagtca
     2221 acctgagcag aaagcccagg gctatgttcg actcggacgg ttgccttgtg tactttcgcc
     2281 tgcttaatct ggcgacaata ggtctttact aaactgtagc ttcctccaaa tcctttgtgc
     2341 tcaataaact tgaaaatgct gtaagcactg cattggtctt tgattttgtc ttggatgatt
     2401 tctctgtagg gatctaactt acttggacgc ttcttacgtt ctggaggttt tgattcttta
     2461 tcttgggctt cttcgtaagc atgcttaact gttcggtagt cgcagtcgta ttgttttgct
     2521 agtgcagcat aattgggttt gatttcattg atcacgtaga tcatcactcc ttcgcgtatg
     2581 ccttttctca tccctgagcc tccatttcat ttgggataag aagagcatac aaaaatgtag
     2641 gtaaaccaac aattttaaat tgccgttttc ctacatttta aaggtgccgt tgacaactta
     2701 cttgtaaaaa ctattttttc cttaataaat caaggaacgg atttgcctta aatagagtag
     2761 taatggttaa ataaataggc aaagcaatag caaactgtat caaattagtc aaaatactac
     2821 ttgataaatt acttgttaaa gaacgtgtta ctatgcacat aagtattccg cctagcaaaa
     2881 agattccaat tttacgatat tcaaatttaa aatctgtact ctttaagaaa gatctagtcc
     2941 ttacaaaagt tacaaaacat tctgctaaaa tattcgaaaa aactacacca aaaaaaccaa
     3001 tagttggtaa caatattaaa ttggctacga tattgatgat ggccccaaca attactgatt
     3061 tgttatattc cttgacgtgc cctatcggca ttaaatattg tctggaaatt gacataccta
     3121 acggaataat tactattaga gctgaaaaat aaggaataac attattaatg tatataaatt
     3181 tttctccaaa aaaccacggc actaatttgt cataaacagc tagcatacca aacataatag
     3241 gtattgaaaa gtataattgt aagtgtatag ttttctccat cattcgaacg attctagtca
     3301 cattttcttt tgcaaaaaag ccagtcatat gaggcaataa cactaagtct aacgtagtaa
     3361 taatggttat aaaaacggta ttcaattgca atgagttagt gaaatatcca accgccatgc
     3421 tacccatagt aaatcctaaa agcgttttat tcaaatttgt atacaacata atagctattt
     3481 gcggtataaa atattcaaat gatcccttta agtgattaaa gctatttctt atacttactt
     3541 ttacaaaatg aatatactta ggaataaaca gccaaacact tatttgcgaa catactaaac
     3601 ctaacgcttg aataagtgta tattttaatg catctccttc atttttaatc aagaaaacaa
     3661 ttaaaacaaa cgtaacaatt tgtaccaata aattagaaat actagttttt ttaaaatctt
     3721 ca
//
LOCUS       DAEGIQ010000180         3663 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000180 DAEGIQ010000000
VERSION     DAEGIQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.189"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..276
                     /locus_tag="KMT45_002792"
     CDS             <1..276
                     /locus_tag="KMT45_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBE7006214.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="KMT45_002793"
     CDS             419..640
                     /locus_tag="KMT45_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7006215.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="KMT45_002794"
     CDS             656..1498
                     /locus_tag="KMT45_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006216.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="KMT45_002795"
     CDS             1561..3363
                     /locus_tag="KMT45_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBE7006217.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3422..>3663)
                     /locus_tag="KMT45_002796"
     CDS             complement(3422..>3663)
                     /locus_tag="KMT45_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBE7006218.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          746 g         1092 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actactggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaataga tacccgtggt gctagttaat
     3421 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
     3481 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
     3541 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtccca actgtttttc
     3601 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
     3661 gaa
//
LOCUS       DAEGIQ010000181         3639 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000181 DAEGIQ010000000
VERSION     DAEGIQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.190"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            21..1787
                     /locus_tag="KMT45_002797"
     CDS             21..1787
                     /locus_tag="KMT45_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBE7006219.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            1810..3618
                     /gene="uidA"
                     /locus_tag="KMT45_002798"
     CDS             1810..3618
                     /gene="uidA"
                     /locus_tag="KMT45_002798"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBE7006220.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         1317 a          452 c          745 g         1125 t
ORIGIN      
        1 ataagtagag gagaaagaaa atgactataa aagtagattt acgaaaaaaa ccatataatt
       61 tagatgaaga tgctatttct tggattgaag agacgattga ttctatgact ttggaagaaa
      121 aaataggtca attgtttgtt aatatgggat ctagtcgtga taaaaattac ttgagtaata
      181 tggtcaatca gtaccatatt ggagctgtac gatataatcc aggaactgct gatgaggtat
      241 atgatcaaaa ctatatctta caaacagaaa gtaaaattcc tctattaatt gctgctaata
      301 cggaagcagg tggtaacgga gcttgtacag atggtactga agttggtgta gaagtaaaaa
      361 ttggtgcaac aaatgatgcg aaatatgctt atgaattagg gagagtttct gggattgaag
      421 catcggctat tggatgtaac tggagttttg ctcctattgt agatattaat agaaattgga
      481 gaaatcctat catttctagt cgatcttttg gacaaaaccc tgataaagtt ttagaactta
      541 gtttagctta tatgaaaggt attcaggaga gtggaatttt gcctgcagct aaacatttcc
      601 ctggtgacgg tattgacgaa cgagatcagc atctatcttt tagtattaat agtttaggta
      661 cggaagaatg ggataattct tttggaaaag tatactcagg cttaatagaa gctggattac
      721 cttcaattat ggcaggtcat atccatatgc cagcttattc aaaacatttt aatcctgatc
      781 tgaaagatga agatttactt cctgcaactt tatcaaaaga attaatcact gacttactta
      841 gaggaaaact cggatttaat ggattagtag ttactgatgc tagtcatatg ttaggaatga
      901 ctggggcaat gaaacgatca gaattattgc ccacttctat tgcagctgga tgtgatctat
      961 ttctattttt taatgatcca gatgaagact ttaattatat gatggaaggc tatcgtaaag
     1021 gaattatcac tgatgatcgg ttattagatg ccttaaaaag aattttaggg ttaaaagcta
     1081 aattgggatt acacaaaaaa gcaaaagaac aaattttatt acctaagcaa gaagctctgg
     1141 aaaaaattgg tttaattgaa aataaatcaa tttttaaaga ggtggctgac caagcaatta
     1201 cattagttaa agataaacaa gatatttggc ctattgtacc agaaaaatat aaaaggattt
     1261 tactagtaga tgtacatggg gtaaaaggtg ggtttggtga agtagttgct ggaaatagac
     1321 ctcaagccat tgatttattg aaacagattt tgtcagcaaa aggatttgaa gttgaaaaat
     1381 acatttcacc tgaagaagaa ttgaaaggat tatcgcaaga agaaaaaatt aaaaatatag
     1441 ctggaatata tgctgctaag agaccaatta aaaatttaac taaaaaatat gacttgatca
     1501 ttaatatagc taatgttaat ccaggcacag ttcaacgaat tgtctggcca gcaagtaaag
     1561 gaactccgga tataccgttt tacattcatg aaattccaac aatttttgta tctgtacagt
     1621 gtccgtttca tttggctgat gtacctcaag taaagactta tattaatact tatgatggaa
     1681 aacaagaaac tttagaaata ttggtggaca aactaatggg tctttctgaa tttaagggag
     1741 taagtccaat agatgcttat tgtggtttaa aggatacatt tatttaatat ttagatagga
     1801 gtggtttata tgttgtatcc cattatgaca gagacgaggc aagtaattga cttaagcggt
     1861 ctctggagat ttaaattaga tacaaatgat gacttacagg aagaacttgc tttagaacca
     1921 ataaatgaaa aagagagtta tccgatttca gttccctctt cttataatga tttatttgaa
     1981 acagagaaca ttagaaatca tgttggatgg gtgtggtatg aaaaagaagt gactcttcct
     2041 aaaagattat tagaagaaag attagttctt cgttttggat ctgcaacaca tgaagcaaaa
     2101 gtttttttga atggaaagtt tattactcaa cacaaaggtg ggtttacacc atttgagtct
     2161 gaaattaatc ggtttgtaaa agacgggaat aatcgaatta cagttgctgt aaacaatatt
     2221 attgacgata gtactttacc tgtggggatt atgaaggaaa cagtacagga aaatggggat
     2281 ataaaaaagg aaaacttagt taattttgat ttcttcaatt atgcaggtat tcatagacct
     2341 gttaagttat atacaactcc gaaagactat ataagtgata ttactattaa tacaagaata
     2401 actgatttgg atgcaagtgt agaattcaat atcgatacag aatggaccgg aaaaatctta
     2461 atagcgatat atgacgaaga ggctcaactt gtagctgaag ctgaaggaaa gaaaggaatg
     2521 attcagctta agaatccaat tttttgggaa cctctagacg cttatttata tagtatgaaa
     2581 attagtttaa ttgatgataa taaagtgatc gatacctata cagaagaatt tggtattcgc
     2641 agtgttcagg ttaccaatgg caaatttttg attaataata aaccttttta ttttaaagga
     2701 tttggtaagc atgaggattc gtatgttaat ggacgtggtt tgaatgaggc tgtaaatatt
     2761 aaagatttta atttaatgaa atggataggt gctaattctt ttagaacatc acattatcct
     2821 tactcagaag aaattatgcg attagcagat cgagaaggta ttgtagttat tgatgaagta
     2881 cctgcagtag ggttacattt aaatttcatg gccactttat tagatgataa tgtggaaaaa
     2941 cataatacgt ggaaagagat acgaacagaa aattctcata aaacggtgat caaggaattg
     3001 atagaacgcg acaagaacca cccaagtgtt gtaatgtggt ctattgcaaa tgaaccagat
     3061 tcggattccg aaggtgctaa agaatatttt gaacctttag ttgaattaac taaagaactt
     3121 gatccgcaga aaagacctgt aacaatcgtt acttatctaa aatcaacgcc agatgtttgt
     3181 aaggtaggag atattgtcga tgttttatgt ttaaatcgtt attatggttg gtatgtttcg
     3241 ggaggggatc tggcacatgc aaaatatctg ttgaaaaaag agctagatgg atggatggaa
     3301 agatgtcctg atacgccaat aatgtttaca gaatatggag cagatacagt agctggattt
     3361 catgatacag taccaacaat gtttacagaa gagtatcaag tggcttatta cgaagcaaat
     3421 cacgaagtaa tagatacatg tgaaaatttt gttggtgaac aagtgtggaa ttttgcagat
     3481 tttgcaacaa gtcaagggat tttaagagtg caaggaaata agaaaggaat ctttactaga
     3541 gatagaaaac ctaaaatgat tgcttactat ctacagaaac gatggtcatc aatacccaat
     3601 ttcagttata aaaaataatt tggatttttt aagaatgtt
//
LOCUS       DAEGIQ010000182         3550 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000182 DAEGIQ010000000
VERSION     DAEGIQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.191"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            64..1395
                     /locus_tag="KMT45_002799"
     CDS             64..1395
                     /locus_tag="KMT45_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBE7006221.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1361..1711
                     /locus_tag="KMT45_002800"
     CDS             1361..1711
                     /locus_tag="KMT45_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006222.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1803..2222)
                     /locus_tag="KMT45_002801"
     CDS             complement(1803..2222)
                     /locus_tag="KMT45_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBE7006223.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2223..2567)
                     /locus_tag="KMT45_002802"
     CDS             complement(2223..2567)
                     /locus_tag="KMT45_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBE7006224.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2917..3513
                     /locus_tag="KMT45_002803"
     CDS             2917..3513
                     /locus_tag="KMT45_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBE7006225.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          933 a          645 c          646 g         1326 t
ORIGIN      
        1 tgatagttgt ccaaatcttt catttcaagt tatagttaaa gaaaatgaaa aagaaggtgt
       61 gtaatggaaa acgaaactga cgttatttat attcatcctc aaaaacgaat cgtttcacag
      121 aaaagaaagt atttttatct tggttttaca ggtgtttttt ttctttttat tggcttgctt
      181 tctaatactc ctactgataa ttggagtgga ttgctaacta ttcttacctc gccaagcaat
      241 cttttgacag attattttgc tttaggtgtt ttcggaagcg cattcataaa tgtagggatt
      301 ttgaccttac tttctgtgct tttagcttat cggcacaagg tgattttaaa cggtccctta
      361 tttgcttcga ttctaacagt taccggtttc tctttttttg gcaaaaacct ttataattct
      421 atctcaatca ttttaggtgt gtatctatac gctgtatttg tcaataaacc tttttctcag
      481 tacatcatga tcggactgtt tggtagcgta ttatcaccgg tcgttagtta tatcactttt
      541 ggtatgcgtt ttcctttact gataggtatt ttattgggga atttagctgg catagcaatt
      601 ggtatacttc ttccgccctt agctgctcaa actttagttt tccatcgtgg attcacactt
      661 tataatattg gattcacctc tgggttgatt gcgatgactt ttactgctgt tttgaggtta
      721 ttcagctatt caatcgtcga aaatacattg gttttcaatg aatatcactt tcctctcatt
      781 tggattattt ttggcttttt ttcgctgaca gtcggaattg gtttctatta taattccttt
      841 cggctttcgg ggattcgaga aattttcgat tcatctggaa aattgacaac tgatttcatt
      901 gctaattctg gtattggtgc tactttgatc aacatgggct tagttggtct gatgctgtct
      961 tcttatgttt tgctggttgg cggtcagtta aatggacctg tcatcggtgc tattttatct
     1021 gcggtgggtt tcagtgcttt tggttgtcat ttaaaaaaca gttttccgat actagtcgga
     1081 atatttattg cttctttgtt tggaactttt catgagatca ccagtactgg catgcttgtt
     1141 gcagctgtgt ttgggacagg tcttgcgccc ataagcggtt tttacggcag tttttacggt
     1201 gtaatagctg gtgtgctaca tattgctctt gtccataacg tcagcacttt gcacggagga
     1261 ctaaatctat ataacagtgg tttttcaact ggtttcgttg caggtatcct tgtaccgata
     1321 ctagataact ttaccgctgt cagaaaggaa aaaaagacac ttggaaaaag aattatcaaa
     1381 aagaatcatc gatgagcttc ttacttattt atattcccat gaaataactg aaatacgtat
     1441 tggagtagaa tttgctgagg aagggctttt catagagata cagggtacgg cagaaactca
     1501 gccagaagat ttagatgtac ttgctgattt gctgaacaat cctcgggacc ctagtatgga
     1561 aatctactat caagaactat taggtaatcc ccgacatgat cgggaagatt atcatctgtt
     1621 aggtagtttg attgatgacg tggaaatcat gtatgcccag ccgattatca atattaagat
     1681 ctttcgacgt gcttctcatt cctattatta accctatttt ctatttataa aagactgaat
     1741 caaaaaagca gctcctgaaa gttcgattaa aatctacttt caggagctgt tcaagcttga
     1801 cattattgat tttccaatcc ttcatacact tcatctgtta caggttctaa aaactctgaa
     1861 ttaccagcag tgatcgccaa atgttcaaac caactgtctt ttgtcgcacc atgccaatgt
     1921 ttaatccctt tgtgcgttac gacgacatct ccagccatta atcgtcttgc tggctttcct
     1981 tcttcctgat accagccttc tcctccggtc acaagtaaga tttgatagcc gtctgtatgg
     2041 acatgccagt gattgcgaca acctggttca aaagttacat tacctacacc aacagaaact
     2101 tcgggatcat tcactaacat attcagatag ctttgcccgg taaatacttg agcgtaagct
     2161 tcgttttttt cacctgctgg aaaaatcacg ccattgtgta tttcttcgtg ttttgtcatc
     2221 atttacgcct ctttcttatc ttcgctattt tcttctaaat agatttcttt tgctaaacta
     2281 aaagctgacc aagcttttgg ccattctaca taaaaagcta aatgggtaat gatttctgct
     2341 atttcttcac tagtaatgcc attttgcttg ccaatcttca gatgagctgg caattgttca
     2401 aaatttcctc ctgaaattaa tgcagatatg gtgatgatac tacgctgatg agcagataat
     2461 tcttctctgg accatacttg tccaaataaa acatcatcat ttaattctgc aaattttggt
     2521 gcaaaatctc ctaattgatc tcgtcctgcg gtttgttttt ctgccatttc tcttattctc
     2581 ccttcacgac tgttaggtga ttcaactatg gttctaaggc aactttattt tctattgttt
     2641 cttcgcattt cattttaaat gtagtctttc agcctgtcca atgcttagtt tattcagtcg
     2701 atatgctttt taagcatagt tgctaacgct ttttttattt taaaaaaaac aagttattaa
     2761 ataagtcttt agttggatgt ttttttgaga tcaatctttt atacttatga tgaacttaca
     2821 aaattgtttg ttttctgtca tatcaaaaaa tggcgcagtt agttgctttt ctagtaaagt
     2881 atagttaaca aagaacctct aaggagttga tgaataatga atattgctgc ctctcgaaaa
     2941 ctgataaatt tcatatccat tatcggatta ggcctatcaa tcgcgctgac gatttatttt
     3001 attaatttag gtgttttcaa agatttgaat gcgttaagag gattagtagg tgattccatc
     3061 attttaggac cgattatttt tgtcttcatc caaatcctgc aagtagttat tccaatcatt
     3121 cctggaggga tcagtactgc agctggtgtc ctgatatttg gcccttatgc tggatttatc
     3181 tataactata ttggaatctg cattggttcg attatcattt ttctgctagg gcgtcgttat
     3241 ggaaagccat tcatactttc catgattagc gataagacat ataacaaata tattggctgg
     3301 ctggataatc aaaaccgttt tgagaagctg tttgctctag ctatttttct tccagttgct
     3361 cctgacgatg cgttatgttt gatggctgga ttgacaaaca tgtcagtgaa acgatataca
     3421 ctaatcattt tgattgctaa accactgtcg attttcttat atagtatggc cttgatttat
     3481 ggaggtcaat atctaagtgg tcttcttgga taatatttat tttgtaaaat tagtcattga
     3541 ttgacaatgt
//
LOCUS       DAEGIQ010000183         3543 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000183 DAEGIQ010000000
VERSION     DAEGIQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.192"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(560..1600)
                     /locus_tag="KMT45_002804"
     CDS             complement(560..1600)
                     /locus_tag="KMT45_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBE7006226.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="KMT45_002805"
     CDS             complement(1949..2275)
                     /locus_tag="KMT45_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006227.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3065)
                     /locus_tag="KMT45_002806"
     CDS             complement(2262..3065)
                     /locus_tag="KMT45_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBE7006228.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1084 a          584 c          487 g         1388 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aac
//
LOCUS       DAEGIQ010000184         3485 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000184 DAEGIQ010000000
VERSION     DAEGIQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.193"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            152..385
                     /locus_tag="KMT45_002807"
     CDS             152..385
                     /locus_tag="KMT45_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBE7006229.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            382..789
                     /locus_tag="KMT45_002808"
     CDS             382..789
                     /locus_tag="KMT45_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBE7006230.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(1926..2444)
                     /locus_tag="KMT45_002809"
     CDS             complement(1926..2444)
                     /locus_tag="KMT45_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBE7006231.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1183 a          590 c          528 g         1184 t
ORIGIN      
        1 gaagggagca atctaaattt tttgaatttt cttaattaat tatatagaaa gaacaatctt
       61 tctatagagt tagacatgag agggactatg cttgatttaa aatcaacaag cgtatatact
      121 gtatatacaa agaaaaagga gggagaagac tatggcaatt aaagaaagaa aatttaggga
      181 agtggggaac tcagtagtat taactatttc aaaggagttt cttaaagaaa atgggttaaa
      241 tcccggagat acggtttttt tagatgaaga aaaacttcga gcggcaatta caaaaaaaca
      301 agccttatct gatgaaattg atttagcaat atctcaagca ttaactgaat atgatactac
      361 ttttagagag cttgtcgata aatgagtatt cattacctag aagccaaaga tattataaaa
      421 atgaatgtaa tccaaataaa aaaatactct cctaatgaac cccttggtgt aaaggatgcg
      481 aatgctttag aaatgtgtgt agggcaactt cgagcctcag cttttggtga agaggtttat
      541 ccttcgattt atgaaaaagc agctatccta cttatacaat tgataaagaa acatcctttt
      601 cacaatgcaa ataaacgtac tgctttctta gctacttttg tctttttgaa aatcaatgga
      661 cagcttttag ctattgatca aaaagatgct gtaaatttag cggtatatat tgctacgtat
      721 gataaggatt tcgatcaact aaaatatgaa gtgatcaaca ctataaagac acatacgcag
      781 cccatttaag gagttactgt atgtgtcttt atagggttat tagctgctat agcgaaataa
      841 ttgaagtaaa ttacaaatag ctataattca aatccaacta tatagaaaac gtaaattttc
      901 tatatagttg gattaagata acgactattt attttatcat atttttacta taccctatac
      961 ttttacttgg tatatttatg aataatcgca aatttacccc tatactacta gtttgtaagt
     1021 tagattaata aaaatactaa agttatttgt tcttttatta aatacaccta caacttataa
     1081 attgatggta tttagataac acgaaagaat aaaaataaca gctgatagta aagtttgttg
     1141 acgattgcta ctcaatcgaa agtgagttag tgcgtatgaa cttgcttctg aaaacttatg
     1201 aaatgaaaga ccttttacat cttgcagaaa tcggtactgg gattaaacta agcaataaaa
     1261 actaatgttc ccctcattct ttgaacttct actatttgat ttatttattc tgataaatga
     1321 cgatttcacc ttcaggatct tcaaatcccg tatagaattt atttaagatc tcttcagtat
     1381 cagtaatagg ataggcgata ggccacatac cctttgatgg ttagaataaa ctgctcaaaa
     1441 tctgtatcgt atgttggctt tagcatatta acaaaggcta tctccaaatt ataaaccata
     1501 agcgttgaat cgttcttaac aatttcaatt cttcctaacc caaagtcaga agatataatt
     1561 accggacttg ccttaccctt catgtgggtt gttgagattc catatttaaa gggaattgtc
     1621 ggtactatcg gcggtctgaa aaacatccca aaaataaaaa aatctttccc ctgctagtct
     1681 cctaagtccc tttgtagcgg atatggcacc ataatctcat tttatcgaag tgagtcatgg
     1741 tttatctgtt ggccaaccaa aattgataca atctaacgat gcaccctcta cggcattcta
     1801 ggggagtgca cttgcattca cccaataaaa accggcagtt agcaaagaac ttatgtagtc
     1861 gtatgttctt actgttaaca tacaagaggt ctcctttcca caattgctta gcagtaaacg
     1921 ggaatttaca aagctaatct cttccatttt cttatctttg ttctaaaagt cccaaatgga
     1981 gcaactgtat ttacatgaat aaatttatat acttcccata cagccgtttt ggttgcctcg
     2041 tctgcccatt ttcttttgtg tgcagtgaat agatcttcat cactcattga atcaatcatc
     2101 atgttaatat catcaatatt acgattgaga atttcctcta attctttcaa tggtagatgt
     2161 gcataggtat ttgtaaacca cttatataac tctcctaact gattccattt aaattgatca
     2221 gaaggcgttt taacagataa tccatttctt tcatcatttt cccattttag aattaaattc
     2281 gtccaaccaa cttgatatgc taaattttca gcaggtgttc tatccacttc ctcaactctt
     2341 ttatccttca attcttcagg aatagtttca aattctaaaa tatatttagt ataagtccta
     2401 tttatttctt tttttaattc ttctttattt ttatatgttc tcattatatt acctccataa
     2461 attcctaatt gtctccttta attcatctat aatttcatcc acgatatttt aatccgttct
     2521 acaatgcata tctgatatgc attgactgtc taactcatat gtgaatgatt tataaaatat
     2581 cccaacgtct catctaaaga aatgttgatc gtcagcaagg agctaaaaaa tacgaatata
     2641 tttcttttgt agtaaaatcg gactggtttg catatcattt tcttcgtaaa tatcaaaatc
     2701 atgttaacta ctatcaatta ctaataaaaa ctaccacttt aagcgttaag aactgttaag
     2761 tttagttaag tgaggatagt tcacaaaatc cattgttaaa cttcacgaaa gtggtaaaac
     2821 tcaaaatgct ttatatagga ttacagcatc gctctttctt cgattgcgcg ttgggtaaaa
     2881 aatattatga agtaaaaatt gacgatgaca cgttccttac ggctcatcag atgaaacagc
     2941 tacaaaaacg aaatgatcaa ctcgaagaaa atttaattct aaaaaagcga ttgccatatt
     3001 cacgcctcgc tcgacgaact attaaaggtc aagatttacc tgaagatcat cctttttagg
     3061 aaattgaagc tttttatgtt gcacaatgca tttataatac tacaatgatg ttttcaccag
     3121 atattcttct agggtgcggt gtaatgaaac agatacactt gcaaaagaaa attcaccgcg
     3181 aatttactaa aattatgaat aattatgtta atcttccgaa atttttttcc aaaattaagg
     3241 gatcaagctg gtgttataga ttgtttaagg ttggcaaaaa aataacttct ttttaaacaa
     3301 ttatagaaaa ccgatactct gttaagttgg ttgacacggt gtcgattttt atgactttct
     3361 tattctgaaa cttcactaac ggacagtacc taaaaagtct atagtatgga cgtttacgag
     3421 tactatttta tcaaaaatgc tgctcatttc aacttatatg ttttagtgaa ctacccccac
     3481 ttagc
//
LOCUS       DAEGIQ010000185         3449 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000185 DAEGIQ010000000
VERSION     DAEGIQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.194"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(150..1337)
                     /locus_tag="KMT45_002810"
     CDS             complement(150..1337)
                     /locus_tag="KMT45_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBE7006232.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1447..1824
                     /locus_tag="KMT45_002811"
                     /pseudo
     CDS             1447..1824
                     /locus_tag="KMT45_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /gene="yfcC"
                     /locus_tag="KMT45_002812"
     CDS             1872..3272
                     /gene="yfcC"
                     /locus_tag="KMT45_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBE7006233.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            3305..>3449
                     /locus_tag="KMT45_002813"
     CDS             3305..>3449
                     /locus_tag="KMT45_002813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006234.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          978 a          652 c          684 g         1135 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgctttta
//
LOCUS       DAEGIQ010000186         3385 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000186 DAEGIQ010000000
VERSION     DAEGIQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.195"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            137..1177
                     /locus_tag="KMT45_002814"
     CDS             137..1177
                     /locus_tag="KMT45_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBE7006235.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2001..2333
                     /locus_tag="KMT45_002815"
     CDS             2001..2333
                     /locus_tag="KMT45_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBE7006236.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2949..3290
                     /locus_tag="KMT45_002816"
     CDS             2949..3290
                     /locus_tag="KMT45_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBE7006237.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1257 a          457 c          643 g         1028 t
ORIGIN      
        1 ttttatttct gattttaggt agtatttaat taaaaaacag ttttaatgcg tttgcatttt
       61 ttatttatct ttgctatttt cgaaaagagt aggtaatttt acataaataa aacaacgtga
      121 aagtagagga tctacaatga ctgattttaa attttttaag gcggatagag tttacaacga
      181 attattttat caatttccaa aagtctttat tgtttctgac gaatacaaaa aaatgaaaga
      241 ttcaactaag attgcctata tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg
      301 gcaaatcgat gaagaaggta atgtttattt tacttatacg acaaatgaac tatgtagagt
      361 attaaactgc caaaaacaaa aagcgatagc aatcaaaaaa gagttggaat cctttggttt
      421 attattacaa aagcagatgg gatttaacaa acagttaggg aaaaataatc ctaatagact
      481 atatctagca gaattaaaag tctcagaaaa tgatatctac ttactcgaaa aatttgatag
      541 agagaatagg gaaaacgttg ataaatcaga gggtatgaaa atcataccca ccctcgacga
      601 aaaatcagac gctgaatccc ttggggctca agagggtatg aaaatcatac cgtgccaaaa
      661 cgttgataaa tcagagggta tgaaaatcat accagaactt aataataata tattagacac
      721 taatagacac aatatagaca ctgaaaaaga ccgcctacaa gatcaattgt tgttagacaa
      781 ttttgagaca attatgacaa acgacagcat tgctacgttt gtccctgaac gagtattaaa
      841 tttgataaaa acattttctt caagttacag tgaagctcaa aaaaccgtcc agactattca
      901 taatgcaaag aaaaaagctg aaatagaaag tgatatttcg atagtttttg aagaactcga
      961 tagttattat gtcaatgcag aacaagaatt atacacgaca ctgttaaaag cctatcaaaa
     1021 attaaaaacc gaaaaagtcg aaaatatcca gaacctgatt tttgtctatg taaaaaattg
     1081 gtttatcgaa aaaccaatag ctgctaaagt atcaagtgaa aaacgtttga attatgcaag
     1141 ctccccaagc actattacga aagactggtt agagtgaaac tttactgaca aagtgattgt
     1201 tgcttcatgg agtaacatta aaattaaaaa ttttttatta ttctaattat attacgaata
     1261 ttacaaaata atagctattt taatagaatt tataagttaa aatattatga taaaaatcac
     1321 aaacgtagcc ttttacgtca taagtgttaa aagaaataat taatttcaaa tatagaaaag
     1381 ggaacgtatg tttgcatact cctttttttt gagttataat aatacgcgaa gagagataat
     1441 ttcattattt aaacaagcta aaaatcttga tattacttct tttcaggcaa tacgaatatt
     1501 attcgcttac attttttgta taaatttaat tgtctgctat gcttgtgatg aattgatttt
     1561 cgttttgcac ttttggatga taaatttgga caattgaaaa tacaaaaaaa agaaaggcgg
     1621 tatgaactcg gaaggctggt aaccttcgtc agcgttcata cacggcatta agatttggcc
     1681 cttaatgcgt taacctttca gtgttacggt attacccgca atggatttag tataacagat
     1741 attcaaaagt gataccactt tttttggctg tgttaaggtt aaaaatacgt gatattacga
     1801 tgaatctatg cctaagcata atgttataaa aatgtccgtg tggctctttg ccgtatgacg
     1861 aacgctgaaa ttagagggaa cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga
     1921 acgaaaatca attctctatt tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa
     1981 gaaattgggt ggtttatttt atgtcatata caggaatttt gagtctcaaa gatatttgtc
     2041 actacggtaa acggtgtacc gctacagaga aaatcacgaa gaaactatca actggccaaa
     2101 gtaaaaccgt tgtgcagtgt aagaaatata taatccagaa agacaaagtc tcagaggaaa
     2161 tgatttatta cattgggaaa caaaagcaaa taattttaaa agatcctatc cccttaaaag
     2221 agctatatcc aacaatcaaa catgtttatg accaaaacgg tgtactgatt ggtcgaagaa
     2281 aaaatggagt cttgcgttgt accgcaaaag ggatggggcg tttgatcggc tgaattgatc
     2341 attctcataa cttgtatttt atcgtttttc gcttgcttga aaatattgtc gagacttcta
     2401 aaggaactta gagtcactag aaaaataagg agtagacttg atctatcaac aattcccttc
     2461 ggggagtgac ggggtaggtt ttaacgattg gtaacgtaaa aaattggtca atcgagtaaa
     2521 tgcgcttgtt tggatagtgg tgaaatgaat ttgtgtaaat aataagcgca gagtaaacaa
     2581 gagggaattt agaagttaga caagttagaa tagcgtctag attaatcatg agcagctact
     2641 aagggatcta gactttgacg caaactcatg gaataaggag taggtcttct cgtaatgggg
     2701 aggacgcaca agaaattgtg tcgtgtgctg cagtacatgt agatacttgc gaggacgttg
     2761 attattggat caatcgagca tttaccgctg tctagggttt ctcgtttcgg acgccataaa
     2821 cgagtcgaaa ttttttatgt atttttaagt aaacgaaaaa cgataaaata tgaacaagtc
     2881 ctgaataaag tgaaaaaatt aatataaagc atagagaaaa tgaaatttac gtaatggagg
     2941 aatgacaaat gaaaaattat gatccgaata tacgattggg cacacatact attaaggttt
     3001 ctttccaacg ttgggattac aaaggatttg tgacgtttag aaggggcgga aattgtaaag
     3061 gattagatgt tttagcatta gacgaggatg atttatacga tcaaacattg actgataatc
     3121 caattggatt cggcttgcta cctgaagatg atgaaggtaa tgaatggttt aaaatgacgc
     3181 ttatgaatga taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt
     3241 atattgtcag ctttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg
     3301 aataaatttt acagataaag gcttagaagg agtcacgacg attaataaaa aggtagtatt
     3361 accttaggca ttgagtaagg aattt
//
LOCUS       DAEGIQ010000187         3366 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000187 DAEGIQ010000000
VERSION     DAEGIQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.196"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..2177)
                     /locus_tag="KMT45_002817"
     CDS             complement(<1..2177)
                     /locus_tag="KMT45_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006238.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="KMT45_002818"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="KMT45_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          938 a          744 c          537 g         1147 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt gcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaa
//
LOCUS       DAEGIQ010000188         3307 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000188 DAEGIQ010000000
VERSION     DAEGIQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.197"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(63..404)
                     /locus_tag="KMT45_002819"
     CDS             complement(63..404)
                     /locus_tag="KMT45_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBE7006239.1"
                     /translation="MKPNEIVTVYAAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
     gene            complement(394..675)
                     /locus_tag="KMT45_002820"
     CDS             complement(394..675)
                     /locus_tag="KMT45_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBE7006240.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDKPVDVLDTDEKLEEWFNEA"
     gene            complement(782..1366)
                     /locus_tag="KMT45_002821"
     CDS             complement(782..1366)
                     /locus_tag="KMT45_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006241.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            complement(1383..1622)
                     /locus_tag="KMT45_002822"
     CDS             complement(1383..1622)
                     /locus_tag="KMT45_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006242.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1641..1889)
                     /locus_tag="KMT45_002823"
     CDS             complement(1641..1889)
                     /locus_tag="KMT45_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006243.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(1904..2470)
                     /locus_tag="KMT45_002824"
     CDS             complement(1904..2470)
                     /locus_tag="KMT45_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006244.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            complement(2471..3016)
                     /locus_tag="KMT45_002825"
     CDS             complement(2471..3016)
                     /locus_tag="KMT45_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006245.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3042..3182)
                     /locus_tag="KMT45_002826"
                     /pseudo
     CDS             complement(3042..3182)
                     /locus_tag="KMT45_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT          945 a          754 c          348 g         1260 t
ORIGIN      
        1 ataaatacgt ttatataaac gtatatctta atatacatat aaacaaaaaa agaaaagcct
       61 tgttagactt ttctttgtaa tctctcgata aacctattta gctttttcgt atcaatagct
      121 gatagcatac caaccttttt aaatgatatg ttctctcggc ttttgggaat agcctttaaa
      181 gaccctacgt caatccatga ttgcttcttt agacctactt tttcccattc tgcaatagga
      241 aaatacactc tctttatttg gtctgattta ttctcatatt gacttgtgat tccaaaaaat
      301 tctactctat cttctttatc actaaccacc aaaataggtc ttcttttacc accattttta
      361 tctgtaaatg cagcatagac cgttactatc tcattaggct tcattgaacc actcttctaa
      421 cttttcatct gtatctagaa catcaactgg cttatccata gttagctgtt gcaatctgat
      481 tccttgcttt tcctcttcgc ttagctttat ctctactgga aactcacctc tagcgaccac
      541 ttgcttataa aggattgtga ttgctgttgt tggatttaac cctagctctt ccaatatcat
      601 ttctgcgttt tcttttaaat ccttgtctat tccgacttga attctgcttt tttcttttgg
      661 attttttacc ttcatttcgt tcacctcttt tgagtataat atactcatta ttatatccat
      721 taactacatt tatgattata ttatactctt aattagctac tttgcaaaca atccaatcaa
      781 atcaagattc tactttactt tttatacgtt ctttcatctt ctggtatgca acctctactc
      841 tttggacatc tatatccaat ccacttacaa ccgtacacat accaatctca tcgctatccg
      901 acactttatc atattcttct tcggtgattt caataggcat atacccatca ttaaagcata
      961 tctcatcatc tgaatagttt tcccattcct cttcatcatc ttctgaataa gtgacaaccc
     1021 tatctgcccc ctgttctacc atatcatcgt aactatcagc aacatactgc gcttttaatc
     1081 ctttcagttg taagtcatat aaaccataca acctcttact atcttgttca ctgaataaaa
     1141 ttctttgacg ttctaaaatt aattctggtt catcttccac ctcttcatct ggttctgtaa
     1201 aataccattc gataaaataa tatctaatat cctcattcga taaactttca aagtccatat
     1261 tctcctcatc aaatccatca aaagcgccac tactttgcat accattcaac caattaatgg
     1321 ttctcaatct tgcgtattca cttaatacca tcttattctc attcatggtt cttcacccca
     1381 tattaattat tttcaaattg acaaatcaat ttattttgag ttaacaaaac taatagctgt
     1441 tcactatcaa aaagaacctt acaagattct ttttctattc tctcttctgg catgtaatag
     1501 ccaatctgtt ttaacatctt atctggataa cctttttgtt tggcttttct caaaagctct
     1561 tcatcattcc caacataata ataagaacac cattgcatac ctttactttt gaattttgtc
     1621 atgttgctac cctctttcgt ttattgaccc aaccatgaaa aaatatattt tgctaaatct
     1681 tctttttcta tcccatataa aacattctct aaatccaatt tatactcttc atcaacaagg
     1741 ctttctgtat tgtcatcata aaggacatag actgcataat ttctttcgta aaaggcatta
     1801 gcaagacttc tgctaatacc taccattcct ttccatgtat aaccaaaatc aatcaaatct
     1861 tcatgatcta taaaatccat tccttccatg ctgattcaca ctcctattct gctgttgtgt
     1921 aatatgcaac gcaatcatct gtccctacta accacacaca ccaatcttca atttcatcat
     1981 cgcttctctt actcaattcc tctttggtac aaaaaactac ttcaccattt tctgtttcca
     2041 tctcataatc ttgtagcatg tattcaaacc aacttctttc ggttggaaat tctatctcgt
     2101 ggcaactctg gcaaataaaa tcaccatatt cattgattcg gtagcaacct tcatcttcta
     2161 caaattcatg attacaacaa acacatctag tacctgttag gacttcaccc tcaatcgttg
     2221 ttctaacggt atcttctctt gctacgtgtt cactgctgac aagccaatca atgattttct
     2281 ttgccaattc ttcatcagaa tcggcaaagc ctttaaacgt gtttctcttt ccttgaaagt
     2341 agtatttgca acttgcggta gtttctatgg ttccataact tttttggaat aattcaatca
     2401 tttcaccatc aatcactttt tgacttttcg cttctaaatt agggaactgg tcttttcttt
     2461 tttcaaacac ttatttcacg ctctcttctt ctaggtttat tccataattt tctagcaata
     2521 atctgtatcc cttaataaaa tctttcgtag caaactcttt tccgctttca caaaacttgt
     2581 ttagtcctat ctgttgaccc tcaaaatcaa acaatcgcaa tctggtttta tcatctagac
     2641 taaatccatt ttcatcgaac accatctcta aatgagcata gccatcattg ttatctgaaa
     2701 acttcatcac atcttcaatc gtttcttgat aaggttcata gcgcccacca aaccctgttt
     2761 catcataagc actcactcca gattcatagg tgacaaaatt gataactcct gtacttctat
     2821 caaagattcc ttttgctaat tcttctcttg attcatctcg taagtcatat gctttattga
     2881 ttaaatatat tccttgattc aaaacgtcca tcaacatgaa tcgaccatat ccccactgat
     2941 aatgaattga cacgcttact ttcgttttgc tgttaccata tttgactgta attaacgctt
     3001 gtgttctttc gcccatcttg ttttcctctt ttctctaatt tttataaaga accattttct
     3061 tgaaatgagt aatagcttgt gtttttactt gttacgatga tatggtctaa caattcaata
     3121 ccaattgttt ttcctgcgtt tttcaaattc gtagtaaaac ttttgtcatt ttcacttgga
     3181 aatggtactt atcaagagta aattacaaaa gaatcattta aatcaatact ttcactttga
     3241 atacatgtat ttgaagtgga aggtacttat ttcaaaaata gtaaaacctg tatcttaaat
     3301 tacttaa
//
LOCUS       DAEGIQ010000189         3239 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000189 DAEGIQ010000000
VERSION     DAEGIQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.198"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            168..1832
                     /gene="alsS"
                     /locus_tag="KMT45_002827"
     CDS             168..1832
                     /gene="alsS"
                     /locus_tag="KMT45_002827"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBE7006246.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1845..2555
                     /gene="budA"
                     /locus_tag="KMT45_002828"
     CDS             1845..2555
                     /gene="budA"
                     /locus_tag="KMT45_002828"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBE7006247.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2685..3182
                     /locus_tag="KMT45_002829"
     CDS             2685..3182
                     /locus_tag="KMT45_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBE7006248.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1101 a          531 c          712 g          895 t
ORIGIN      
        1 ttttatgtaa aaactgtcat tctttttcat tttttgaaac atatggctaa acaaatgaaa
       61 taaaacatgc tatacttttg aatgaaacag gtttagaaac tgtatcactt atttgtgatt
      121 atttctaact aataattaaa aataaagcat aaaaaggacg atgttcaatg gctgaaaaaa
      181 tgaaacaagg ttcgaaatct gtagtagaaa gtttgattaa tcatcatgtt gattatgtat
      241 tcggtatacc aggtgcaaaa atcgatggtg tatttaatga attagaggat caaggtcctg
      301 aattaatcgt gactcgtcac gaacaaaatg ctgcatttat ggcacaagca atcggacgta
      361 ttacaggaga gccaggagta gttatcgcaa caagtggtcc tggtgctagt aacttagcta
      421 caggtttggt aacagcgaca gcagaaggtg acccagtttt agcaattgcc ggacaagtaa
      481 aacgaagcga tctattgaag ttgacccatc aaagcatgga taatgcagca ctgttccagc
      541 caatcacaaa atacagtgca gaaatccaag atccagaaac aatttcagaa gtgattgcca
      601 atgcttatcg tatggctaag agttcaaaaa aaggcgcgag ctttatcagt atcccgcaag
      661 atgtagtgga tgcacctgtc caaggtaatg tcatcaagcc attgagcgat cctaaattag
      721 gttctgcttc tgcagatgat atccgttacc tagctgaacg cattagagaa gcaaaactac
      781 cggtactatt agtaggtatg cgaggatcaa gtgaaaaaga aacgttagcc atcaggcaat
      841 tagttgaaaa gacagcattg cctgttgtag agacattcca agcagctgga gtgatctcac
      901 gtgaattaga agcgcatttc tttggtcgtg tgggtttatt tagaaaccaa ccaggagata
      961 tgcttttgaa acgaagtgat ctagttattg caattggcta tgatccaatc gaatatgaag
     1021 caagaaactg gaatgcggaa aaagatgcgc ggatcattgt gatcgatgaa gcgccagctg
     1081 aaatcgatcc ttttatgcag cctgaaagag aattgatcgg tgatatctca gccactcttg
     1141 atttattgac aggttcatta gagcctcaac aagtgtcaga agatgcaaaa gaatatttag
     1201 catctcttca agcaaaatta acggagcggg acattgttca atcaaaagga gaagcaggta
     1261 tcttgcatcc tttagaagtt attaacacgc ttcagtcaaa agtaacagat gatatgactg
     1321 ttaccgtaga tgtaggtagt cattatattt ggatggcacg ccactttaga agttatgaac
     1381 ctcgtcattt gttattcagt aatggtatgc aaacgcttgg tgttgcattg ccatgggcga
     1441 tttctgctgc attggtgcgt ccaaatacac agatcgtatc agtttccggc gatgggggat
     1501 ttttattctc tgcccaggat ttagaaacgg cggtacgtaa aaaattgaat atcgttcatt
     1561 tgatttggaa tgatggacac tacaatatgg tagagttcca agaaaaaatg aaatatcagc
     1621 gggcgtcagg tgtagacttt ggtccagttg attttgtgaa atatgcagaa gcttttggcg
     1681 ctaaaggaat ccgagctaca agtgtagagg aattagaaaa agctttagaa gaaggatttg
     1741 cgacagaagg acccgtaatt attgatattc cgattgatta ccgtgataat gagaaattgg
     1801 gagaaacaat cttaccagat caattctatt aaggaagtgt cgacatgact gaaaaaatct
     1861 tataccagca cgggacatta ggagctttga tggccggctt gatggatgga acagaaacga
     1921 ttgctcatat attagaaaaa ggaactctgg gattgggaac attgcatggg cttgatggtg
     1981 aagtcatctt tttagatggc gtagcttatc aaggaagatc agatggctca gttgcccaat
     2041 tagatggaag tgaacttacc ccctatgcag caattacaga ttttacgcca gatactagtt
     2101 tttctgtctc aaaaactgca gatggagaac aattgaaaaa agacatatta gtaagagaag
     2161 ccggggaaaa cctatttttg gcagtgaaga taactggatt attcaaaaat atgcacatcc
     2221 gtatcatgcc gaagcaagaa aaaccttaca gaagactagc aaaaatttca gagtctcaac
     2281 cagaattcca acaaagcaat gtccaaggaa cattagtcgg gttctttacg cctgaattat
     2341 tccaaggagt tgctgcagcg ggatttcacc tgcattttat cgatgataca catacatttg
     2401 gcggacatgt aatggatttt gaagtggctg aaggaaaagt tgagattagc cgcatctcgt
     2461 cccttgtaca gcattttcca gttgatgatc cgacttttgt tcacgaaacg attgattatg
     2521 ctgatttagc agcagaaatc caacaagcag aatagaaaaa gacagcgaga tgcgttcaag
     2581 gtgattaacc tgacgtatct cgtttttttg aggagaaaac ctagcgttta caaaaaaact
     2641 gagctataat cttctccata gatcaaaaag gagcggtttt tatcatgtgg acagaaaaac
     2701 agaaaaaaga tgcatacgaa attttattat tacagcaaca aggattatta gaagcagaag
     2761 ataactggtt agcaaattta gcaaattcat ctgcattgtt gaatgagact ttgcctgaga
     2821 ctgtctttgc aggatattat ttattcgatg gtaatgaatt gattttagga ccgttccaag
     2881 ggcgagtttc ttgcacacgt atcaagatgg gaaaaggagt ttgtggagaa tcagctgaaa
     2941 aacaagaaac attgatcgtt gatgatgtca aaacccatgc aaattacatt tcctgcgatt
     3001 cagccgcaca atcagaaatc gtagtgccaa tggtaaaaga aggtaattta atcggtgttt
     3061 tagatatcga ttgcggagta acgaaagggt atgatgaaat cgatcaagca tttctagaaa
     3121 aatatgtaga tcttttatta gcaaatttta atataaatgc tttttcattt attaataact
     3181 aaaatcgtct attgaaacaa aaaagaagag aaatcaagaa taaaaagagc cggaaagac
//
LOCUS       DAEGIQ010000190         3203 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000190 DAEGIQ010000000
VERSION     DAEGIQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.199"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            142..831
                     /locus_tag="KMT45_002830"
     CDS             142..831
                     /locus_tag="KMT45_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBE7006249.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            845..2347
                     /locus_tag="KMT45_002831"
     CDS             845..2347
                     /locus_tag="KMT45_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBE7006250.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2360..2836
                     /locus_tag="KMT45_002832"
     CDS             2360..2836
                     /locus_tag="KMT45_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE7006251.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2933..3043)
                     /locus_tag="KMT45_002833"
                     /pseudo
     CDS             complement(2933..3043)
                     /locus_tag="KMT45_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1058 a          433 c          618 g         1094 t
ORIGIN      
        1 caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact ccatttttat
       61 ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca aaaataataa
      121 aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat ttcaaaaggt
      181 ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga agaaagttta
      241 atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg tgcaaattct
      301 gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg aattattaaa
      361 agagattatc caccagaatc tccattcatt actgccacta tgagagaagt agatgaatta
      421 gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg acatgatgga
      481 ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca gttatttatg
      541 gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat agattttgta
      601 ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc agatatagat
      661 ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag gattcatact
      721 ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt gggtggtgct
      781 attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta gggggagaaa
      841 taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa ttgctatatt
      901 accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga gtgctataaa
      961 tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta tgagttatgc
     1021 tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg ctgtaggact
     1081 tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc tagtatatac
     1141 tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac tagatacagg
     1201 tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc gttatagaaa
     1261 aattgaatta ccacaattct taggtttttt tggaggatca agatttattc cgattgtttc
     1321 tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac caattcaaaa
     1381 tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa cttttttata
     1441 tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt accctttgtt
     1501 ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg ctggagccca
     1561 aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta cttatggtac
     1621 tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag catgttatgc
     1681 aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt attttagcgg
     1741 ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt ttttatttgt
     1801 tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt attttgctga
     1861 tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact ttttattatt
     1921 cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt ttggtattgc
     1981 atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta aggtagctat
     2041 tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca aaaatacatc
     2101 attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa atattgagtc
     2161 tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga ttattcaaaa
     2221 acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg gcatccaagc
     2281 tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat taggaaaaga
     2341 taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag aaacttttta
     2401 tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg tcttttcaca
     2461 aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt atgctccatt
     2521 ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa aaagcaactc
     2581 aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag gagagccttt
     2641 ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg ttgatgtgga
     2701 ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt ttccggagaa
     2761 aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga attcatgtgg
     2821 aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa ataggatatt
     2881 tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt ttttatttta
     2941 ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa aagcttcgaa
     3001 atccattggc atttcgttgg aataccttga tatgattatt caaatataaa atatagagag
     3061 aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac ttaggaaata
     3121 gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac tatataccaa
     3181 atatagtaaa atgctgaaag atg
//
LOCUS       DAEGIQ010000191         3185 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000191 DAEGIQ010000000
VERSION     DAEGIQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.200"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            245..832
                     /locus_tag="KMT45_002834"
     CDS             245..832
                     /locus_tag="KMT45_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006252.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            832..1731
                     /locus_tag="KMT45_002835"
     CDS             832..1731
                     /locus_tag="KMT45_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE7006253.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            1731..2645
                     /locus_tag="KMT45_002836"
     CDS             1731..2645
                     /locus_tag="KMT45_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006254.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            2697..2912
                     /locus_tag="KMT45_002837"
     CDS             2697..2912
                     /locus_tag="KMT45_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006255.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         1228 a          383 c          671 g          903 t
ORIGIN      
        1 acgaaaataa actatcgaaa gtagtatgat tatgatctta aaatatcatc ttaagagaag
       61 aattacgatt gtttatagga tttacaaaat gaataagttt gaagagtgat ttggtcgcag
      121 actagatcac tctttttgtg taaaaaaact ttacacttat ttttgagtgt gttaagattt
      181 tttatataga gtgacggttt cttgtcagag tggatgataa ccatcaatat tggaggaata
      241 ataagtggaa aacaataatg atattagata ttttagcatt ggggaaagag ttacaacagt
      301 aagtgaatta tcaaaggatt atagagataa agaaggaacg attatttctc ttaaaaagaa
      361 tcgaagaggt gtttggcaat atgttgttga tttaggggat ggcgttacag ttgagtgtaa
      421 agatagtcaa ctgatcagaa atgatggcag tcattccatt gaaaaagcaa ttcttgtatt
      481 cccaaaagta tcaagcaaga ttatgaatga ctggttgctt ggaaaatttg gtgagtacaa
      541 taaaaaagaa atcgcagaag tgatcggcaa atcttatcca acagtattag ggtatctaaa
      601 aaagataacg aacaaagaag cgaaagttga ggatgtttta acagtaagag aacttcagtc
      661 attaatcgaa tatcggaata aacaaataac aaatcctgag taccttaaaa aagaagatgg
      721 tctattgaaa caattggaac aggtaaataa aatccggaaa atgcaaaagg tagctccttt
      781 aagcctagat aactttaaaa aaatctattt gaaagagatc gaaaaagact aatgtggagt
      841 gtcactttac cgtttcaacc tgtatctgca ccaagaccaa gtgcaagaag gaatgttgct
      901 gatgcaacta atgacgtagc agagaaaagg atttcaacct attatgataa aaagtatctc
      961 gattatttga aaaaaataaa aagctttatc gaagatcaag gtttgttaga tgatgaattt
     1021 tactcgatcg taaatgatcc gatgggtgca attatggaag tggactttta ctatcagata
     1081 ccaaaaagct ataaaaaagt ctataacatt atgaaaacga cagcgccaga tttagataat
     1141 ttagtaaaaa gtgctatgga tggtattttc aacatctcag ctatacgaga tagccatatc
     1201 acaggcttaa ttgcttttaa atataatgtg gcgaacgaag gaaaaacagt tgtgaggatc
     1261 aaacgatatt cagaatttga gtgggatact tctgaaggat tgaacggaga tatatgggaa
     1321 gctacatttg attttaaacc agtagcaacg ccaagaccaa agtctaaatt tagaggaaat
     1381 gggattatca cttactatcc aaaagagtac aacgactatt tggaaaatat caagaatacg
     1441 cttaaagaga aagatttggt aaatgatcaa ctgaaaaagg taatgaatgc tcctatgggg
     1501 gttatagctc agatagatta tttttaccaa gtgagaaaag ataagaaaaa gcttgattca
     1561 ctgatgcgta cttcacagcc ggatattgat aatttagtca aaggaacatt ggacggaatt
     1621 tttaacaagg aaataggcat caaagatagc agggttgtag ggttaattgc ttttaaatac
     1681 aatacttttg aaaagtcgaa aacaaacgtg agactacagg agatgggctg atgaataata
     1741 atgaaaaaat tcttcatgta cttgatagct ttgaaataat tcaagaagaa ctaaaaaaat
     1801 atcgggatgt tttggaacaa agatatgatt ttgtcaatca gcaaaagagt aatcacatgg
     1861 atttcattct caatatgaat gacttgaaga agaaattagt tgaacgaaaa gaacaagaaa
     1921 agttgatcaa agcttacttt gaactcggtg aaaaagaagt taaaaatgca atggaactga
     1981 atgaagagcg cagggtttta gatcagctgt tagaacaatt attggtgatg tttcaaaaag
     2041 gacgaataga tgaggattta attgaagaag gattgagaaa atatccttca aactcaggta
     2101 tcggaatagt gctgaaagcg attgatgaag aagaaattga agctttcata ccgccagagg
     2161 attttgaatc ggcaatggaa tatataaaat attatagcca agggatcact gcttttagag
     2221 agtttgatcc tgaagatgtt attcatgact tgaacaacct aaaagaatgg tgtgaaagtt
     2281 atggagtcga tgattccgga ttggactact tgatcagtat tatggaaatc gaagaagaga
     2341 tgccggataa gcctgatcca acagatattt tagaattgat ccatgaagct agaaatccaa
     2401 ttgcctatat ttctagagga tatactgtat tggaatacta taagccttat atatcagcga
     2461 tgaaccattt aagacgtgta ttaagggaaa aacgtgaata ccgctcagtg ttgaatgcaa
     2521 cgaatagatt agaaaaagct gtttcagagc tagatgctta ttatcgtgaa cattacctgc
     2581 aagcaggtgg tatgccaaga aatacgaaag caaatatcag taggtatata aaaaaagctg
     2641 agtaggcgga gttagcccat aaaatggcaa aaggtaacct gaaaggggca ttttgaatga
     2701 aaagggatgt ttttgaattg tacgtattag aacgtctaga agacggcatg atggcacaag
     2761 agataattga tgaaattgat gatatgatca gcagatttgg tcaattggag ttgaatgatg
     2821 gaaacaaaaa ttcggtagtg ctaaaacaat tttggttgaa caatttttcg tataaggatt
     2881 ttatcaaaga aaaggcaaat acattgtact gactaaattc acgaggagca gtgatgagag
     2941 cttattgcta agagcagtta ggctcttttt tgtttgtgta aaattatttg ttttataata
     3001 cacatttata cacaaaaaat attgacagat agtacacaaa tgtggtagga tatatttgta
     3061 agcaagttag attaacaagt taaaggaaga gcagttatga aaaaagaaga actaatagat
     3121 atatttcaaa ttgttgaacg agcaaataat atgggaatta tgttttttga tcgtatttct
     3181 ttaaa
//
LOCUS       DAEGIQ010000192         3185 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000192 DAEGIQ010000000
VERSION     DAEGIQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.201"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(402..1016)
                     /locus_tag="KMT45_002838"
     CDS             complement(402..1016)
                     /locus_tag="KMT45_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBE7006256.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1622..1999)
                     /locus_tag="KMT45_002839"
     CDS             complement(1622..1999)
                     /locus_tag="KMT45_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006257.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2299..3180)
                     /gene="galU"
                     /locus_tag="KMT45_002840"
     CDS             complement(2299..3180)
                     /gene="galU"
                     /locus_tag="KMT45_002840"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBE7006258.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          968 a          657 c          493 g         1067 t
ORIGIN      
        1 ttggacaatt ttgttatatc aggtaaactt cattgcaata atgataacaa atgtactata
       61 attactttgt aaagagtttt ggggaagact ctttcatcaa cgtgtattaa gagacaagaa
      121 agaatccata tggattcttt cttgtctctt tttccactac tttttatttt tctgacacta
      181 catattctta taactcattt ctcataactg tgaaacaact tataaaaata acaaacttta
      241 aacaaaacaa gcttaaaatc aaagatgatc catcttaaaa taactaaaac aaaaaaagta
      301 gtcgttgtta atcctcatta ataatcgttt tctttttttc aaacgctcca tttttatgtg
      361 gaacattatt tttttaagga actttgtcaa catgtggata actattttcc acttgtgtcc
      421 tggactagct gttttgctgt aaaatacaaa acttgctgca gcaaaaagat cgttgcaaga
      481 gaggctgttc cgcagatcgc tggatctaga tttggcaaag tcaagactgc atcatgtaaa
      541 ttggcgtggt tgtccaaaac caaatcagca aattcaaata atcgcaaacc actgctatgt
      601 tgcgatttga tacttcgtga caaatcaaat ccagtaacaa tgattaatgg atgaccattt
      661 tctttgatcc actgagctag ttcgatgatt gccgggtttc ttccttcatt cgaaagaaga
      721 agaaaaatgt cttctgattt gacagaaagc tgatcaataa ttgcctgtcc tgcaccttct
      781 agcgtttcat agacacctcc ggctggattt tcaatcaata tacttggtac aagatccggg
      841 gtttgatccc aaaattcaaa tgctacacca cttagtgtgc ggctagcata tatctgcaat
      901 ctaccacctt gattaatttg tttcgcgatc caagtgccag cttgaagcat cagcggctct
      961 tctatcctta atttctcttc caattggtga taatagtcaa aataacataa actcatttat
     1021 ctctcccgcc tagctatact tgacatgaat agaaacagct gcctaatcta aggaagtaca
     1081 taaaaaaaca gtagttagac agtaacacat gtggtctttg ctgtttcaaa cacaaaaaag
     1141 gcattgaacg aatacgccca ataccttgac atagcaaata aaacaacgtg cagaccatgg
     1201 aaaatatcta cataggggta gaattttcac cctagattag gtacaataaa ttaggtcgat
     1261 gcactgcatt gactatggca aaatgatcgg tcagggtgta tgtgtgtgac tggtcattcc
     1321 ctgattctta gcgccaacta agggtcaggg agcctttttt tattcagctg tttctatctg
     1381 aatttattgt aagtaatctc ttatccaagt gcaagcaaga aaatacaaaa aaacattggt
     1441 atgatttata tatatataaa taacataaaa aaaggaatgg cttatcccga tacaacaagc
     1501 attcctttaa aaaagattct tttatataag tgaaaaaagc ttttttccac caattaaata
     1561 aatctctcat ctcaatagtt tatccattat tttatctttt tttgcgtata cctactaaat
     1621 attaccgtct tcttctcgtt cttctacttt gttcgagaga atctgtacta gaaaaagtag
     1681 ttgtatgtct accttttctg cctatccgag actttctttt catatatggg atataaatga
     1741 ttagatacag tacagcaaaa aataaagcaa atacgcaagc ttcccaacta cctaacgcca
     1801 attcaaccat caaggtagca accccaacgg atgctgcata ccagataaaa atcagaaacg
     1861 tttctttttt tctgaccatg cgaaacatga taaatagact aataagcaat agtggcaaca
     1921 caaatatcca attgcttcct tccatcagcc acttgagtat atcaagcagc gactgtgcaa
     1981 cagcgactaa ttttcccata gtgaacacac accttttttt ataattaaaa aattcgcctt
     2041 tttattctac tatgaatctt ctggcttggc tttcatttct cttatgtaat cattttcttt
     2101 tatacaactt aaaaaaaaat taaaccatta gcataatatt ttctgaatct attcacgaaa
     2161 gtctttttct atctttcgtt ttccacgact caactgatct agagtcaaaa aactaaaacg
     2221 acagttgcgc aaatgacgca caagctgccg ctttaataac aaatagcctt tcattctcag
     2281 caggccactt atttccagtt aactttctgt tcttagcttt ttactcaact ggataatata
     2341 tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca tattcaatat ttgtcttcat
     2401 atatcctaac ttatcgccta cgtcgtatct ttttccttta aactctctcg catagaccgc
     2461 ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt atctcgtttc ctgctcctgg
     2521 ttcttgagtt tctaatatct caaatatttt aggagtcagt agataacgtc cgataatggc
     2581 taaattactt ggtgcttttc ccatttcagg tttttctaca aaccctttta ctctgcaacg
     2641 acccttttcg ctatctgttt taggatcaat aattccgtat ttagatgttt cattttctgg
     2701 tacacgcatg acggcaacag cggaagcttg tgtttcttca taatccatca tcaattgttg
     2761 agtcaatggg atatcatctt tcatcaaatc atcccccagc atgacgacaa acggttcatt
     2821 tcctataaat gcttttgctt gaagcacggc atcccccagt cctcttggat gagattgacg
     2881 gatgaagtga agattgactt gagtcgtttc ttcaactaaa gcgagtaatt ctgttttttc
     2941 cttttcccgc aaattgtttt ccaattcgat atttgcatca aagtgatctt caatgggtct
     3001 tttttctttt cccgttacga tcaagatgtc ttcgattccc gaatccaatg cttcttccac
     3061 aataaactgg atcgttggtt tatccacgat tggaagcatt tcttttgcca tggcttttgt
     3121 tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg actgcttttt tgaccttcac
     3181 ttgct
//
LOCUS       DAEGIQ010000193         3104 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000193 DAEGIQ010000000
VERSION     DAEGIQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.203"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            12..>3104
                     /locus_tag="KMT45_002841"
     CDS             12..>3104
                     /locus_tag="KMT45_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBE7006259.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     R"
BASE COUNT         1088 a          535 c          663 g          818 t
ORIGIN      
        1 agggaagaag aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgattc
       61 gtgtcctcgg ggaaggacat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
      121 atctttggag caatttacgt caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ctcctttgac cgataaagag tttgaaacga tcaaaaccga attattgcta cggacgaaaa
      241 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
      301 aacgggaaaa tccgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
      361 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcgcggga tcgacgtttt gacgtcacct tattgatcaa tggcttaccg attatccaaa
      481 tcgagctaaa acaagtaact gcaaaagatg gtttttatca ggcgtttaat caaatcaaaa
      541 aatatgctga agaaggcatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaacgact cggtattttg caaatgcctt accgaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccagatgccc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagtg catgcgattc
      841 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
      901 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
      961 atcgaacgat catgttagtg gaccgcaaag atttagataa tcagaccacc acagagttta
     1021 cgaaatttgc ttcggagttt aatactgggg tttcttctgg taatgccaaa gcgaatagct
     1081 tgatcgtggg aacaggaagt gccaaagagt taagtgaaac tctgttggca gatgccaatg
     1141 cgaatgttgt gatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     1201 aacaggaaga gaaaaaagga accaatcgct tccaaaaatt aatggggcaa catatcgtgt
     1261 ttgtagttga tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa atcaaaaaaa
     1321 tgtttccgaa atccacttgg tttggcttta caggtacacc gattttccct gagaatcgga
     1381 aacaagccaa agggcaattg gcacgtacga cccatgatca gtatggagag gtcttgcaca
     1441 cctataccat caagaatgca ttagaagatg gctcggtttt aggatttcaa gtagagcatg
     1501 aaaacaccgt ggagccgact tcattagaga ataaaatcta tcggaaatta aaagaagtag
     1561 aaacatatgc agagtattca ccggaacaga tcaatcgtat gatcgatcaa atggagtctg
     1621 tcaaaaaaga aagttatttg gaccctactg tttttgaagc agaggaacat atccaaaaag
     1681 tcattcataa aatgttccgt ccagacaatg cctatacaaa atttgatttt cgaaatggcc
     1741 gtccaacgaa gtctgcgatc ttaaccacga gttccattga tatggccaaa aagtattatc
     1801 gggccatcaa agagatgacg aaagaatctg attggttaat aaaagagttt cccaatcagc
     1861 ctatccgtga aggacgaacg atggaggatc ctgatttccc acgaatcgct attacctatt
     1921 cattagaaga aaatagccga gattcaagtg cccaacaaga agaaatgcaa aaaattattg
     1981 aggaatataa tcagtattat gatactgcct ggtcactagc agacattgag cgttataacg
     2041 gagacatcaa taatcgtttg gcacgaaaaa gagcggaatt caaacaattt gggaaacaaa
     2101 tcgatttagt aatcgtagtg gatcgtctat tgacaggttt tgacgcccct acgattcaaa
     2161 ccctatttgt ggatcgtaat ctagagtatg caggattgat ccaagccttt tcacgtacga
     2221 accgtacgta tcctgaaaaa acaaaaggac taattgttac tttccgaaag ccagcgacaa
     2281 tggagaagaa tgtcgaggat gcgacaaaac tgtattcaga agctaaagaa gaatcgggtt
     2341 tgatttatcc aagttaccaa gaatccaaaa aaagattcaa gcaagcatat ggtaagttaa
     2401 aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc cgtatcgagt
     2461 acgtcaaagc atttcaagaa ttgaatcgtg cgtatgaatc gttagtgact tatgatgatt
     2521 acaatgatga catggtgcaa tcttcaactt tgaacaatca aattcaactt ttggaagaac
     2581 aaattggcgt gtacgagacc gtgaagggtt cattgatgga ggaagaacca gaaaaagagg
     2641 gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg tatgatattg
     2701 attctactta tattgatcaa ttactaggaa cttatgctgc gaatagttca gatgcacgag
     2761 aagaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa gaagtttacc
     2821 gacaaatttt aaatgcattt gatgtgggga ctttagatag taatgaagat atctttgtca
     2881 ttaaacgtcg ctattttaca caagcaagtg ataatttgat tcatgccttt tccaatgagt
     2941 ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatatgcca ggtgaagatc
     3001 caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat aaagccaaac
     3061 atccagaagc gaaaccattt aaatacccac aagaaatgaa gcga
//
LOCUS       DAEGIQ010000194         3077 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000194 DAEGIQ010000000
VERSION     DAEGIQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.204"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            116..1243
                     /locus_tag="KMT45_002842"
     CDS             116..1243
                     /locus_tag="KMT45_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE7006260.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLTPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1461..2603)
                     /locus_tag="KMT45_002843"
     CDS             complement(1461..2603)
                     /locus_tag="KMT45_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE7006261.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2608..2793)
                     /locus_tag="KMT45_002844"
     CDS             complement(2608..2793)
                     /locus_tag="KMT45_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBE7006262.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT         1014 a          557 c          486 g         1020 t
ORIGIN      
        1 tgatgaaaat atgcttctat cattacaagc tcgtttggtg taagatgggt ataagtcatt
       61 tatgttcact ctccttgtat gctttagcgg gtattacaat tggagtgtaa cataaatggc
      121 ttttttattt gtctcgctta attatacaaa cggcgaagat atcaatgatg atggctcaag
      181 aaaaaaagtg gagaaagttg gcggagacac ccgaccagag gccgaagctg ctttacgaaa
      241 agttttatca gatattgacg aaacaggaca atacttttta ggtacggata ctcgagtaaa
      301 acaatacctt gatttttgga tggaggaata cgttaaacta aatctaaaat acaataccta
      361 tgaaaactac cgatttacca tcaaaaatca tataaacggt tatttaggaa agaaaaaact
      421 tacggatctc acccctgctc ttttacaaaa tttcatcaat gctgaattta aaaagggtta
      481 ctcgaagaaa acaatgacta ttactcactc tgtccttaag aatgcgctga atatggcggt
      541 ttatccttgg ggattaatca agcaaaatcc tatgctgtat gtaaaaatac caaaatatga
      601 agcacgacca acgactaaaa aagatctaaa aatcatttct cttgaggact ttgatcatat
      661 gctagaaatt actcctgaag gacatccttt ctatattcct ttgaatattg gattttatac
      721 gggaatgcgc gttggcgaag tttgtggtct gacgtgggat gatgtcgatt tttcaaatgg
      781 aacaattact gtagagaaac aaatggtaaa gaatgacggc gcatgggtat atggtacgcc
      841 aaagacaagc agttccaatc gaacgatttt tattggacaa accttgctag caattctgaa
      901 aaaacataag aaacaacaat tagaaaatcg aatgaagtat ggaaagctct acattgattc
      961 aaatgcagta tgtacgaagg aagacggtga gctagttacg ccaagtgtag tgaaatggaa
     1021 tactaggagg atatcgaatg cactctccct ctcttttaac ttccattctc tcagacatac
     1081 tcatgctaca cttcttctcg aaaatggcgc aaaaatgaaa gaaatctctg aacgattggg
     1141 ccacagcaga atttcaatta cgatggatac ttactcgcat gtaacagata agatgagaaa
     1201 tgaaacggtc gatatcatgg aaaatcttag aaagaattct tgatttttgc caccgaaaaa
     1261 aaccaccggt ggcaaaccgg tggcaaatcc atgtaaacat ggtttatttt ttacactgtt
     1321 ttcaaacaaa cccttgctat ttcttcggtt cgtcctcgtc cattttcgat accgacagct
     1381 agcttataac ccaaggtata tcaacataaa aaaataaaaa taggctatga cagcagcgtg
     1441 acagcaaaaa agatggaaag ttaaatatca aacctcgtaa tttcatttgc gagtgcttga
     1501 tttgtttctg gatataagtg tccatatttg tccaaagtag ttttaataga ggcatgacct
     1561 agacgcttag atattgcata gatattttta ttatgttcaa ttaaaaaggc aacatgcgag
     1621 tgtcttaatc catgcatatg aattggtttt actcctgcaa tttcagctag tttttttatt
     1681 ctgcttaaaa tagttcttgg agatggcggt aatccatcaa aagaaaaaac caattctata
     1741 tttttcccaa ttgattgttg agtttttttc catgttttta aatcttcaac ggtttgtctg
     1801 tctaacgaaa ttatccgaat acttgatgta ttttttgttt cagcaaattc ataatcttgt
     1861 cttgtacgga tgtaaattga cttatttacg ctacatctgc catttattaa gtcaaagtct
     1921 ttccattgta acgctaagag ctcctcactc cttacacctg taacaaaaat aaaacgcatc
     1981 aaacgtttat aaaattcttc ataaaaatcc ccgtgataaa cactattata tactttctta
     2041 aattcatcaa ctgtccaaaa ttctacttga ggacgctgag tttttatttt acctatagta
     2101 tctacaggat tttcttcaat aatttcaagt acaattgccc ttttaaaaac tactctcaac
     2161 ttattgaata tctgtttaat ataatttggt gataatggtt gctcaatgcc cttttttaca
     2221 acatggattt tcgataaaaa ctgttgaaat tcctgtatat gaattggtcg aatctttaca
     2281 acctgcattt ctccaaaaaa atccaatgca cgttgtaaaa tatgatctgt tttcatatat
     2341 gttttttcta cagttcccaa cttataccaa ggaaaaaaat actctcgata aaaatcatga
     2401 aacgttattc cttgcagata aattccttgt gaatcagaaa aactttgttt taactcttcg
     2461 agtccccttc tagcttctcc ttgagttttg aagcctcttc tggtcacttg aattcttttt
     2521 ccaaagcgat cttgtcctaa agttgcagaa aaaaaccaag tgcctctttt tctgtcttta
     2581 taaacattcg caattttcct cattttacta ttcctcccaa gaaatatgga gaacttcttc
     2641 taaaattgaa ataggaattc tttttatttt tgaattacga taaaaaaagt atcctttctc
     2701 aactaaaagt tctttacatt ccttgaagat tttattagca tagtactgag aaaaacctag
     2761 attaatcaaa tcttttcgag tagctgtttt cattatagca ccttccttac tttttttcat
     2821 ttatttctat cagctgcttt tgtcaaaagc acataggaat ttcacctctc cccaagttta
     2881 cggcgagcct tacggaagta tcattatccc aactttaaat catcgctttt tattgactat
     2941 aaaaattatt ttagaatatt tatcgctcct tctttacagc aagaaatcat ggcgcaacta
     3001 aaatatgctc cttaaagtgt taacgtactg atagaatcta tttaattttc aatgaacaat
     3061 aggttttcta cctaaaa
//
LOCUS       DAEGIQ010000195         3056 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000195 DAEGIQ010000000
VERSION     DAEGIQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.205"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            101..571
                     /locus_tag="KMT45_002845"
     CDS             101..571
                     /locus_tag="KMT45_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBE7006263.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="KMT45_002846"
     CDS             793..1896
                     /locus_tag="KMT45_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBE7006264.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2117..>3056
                     /locus_tag="KMT45_002847"
     CDS             2117..>3056
                     /locus_tag="KMT45_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBE7006265.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKD"
BASE COUNT         1061 a          515 c          656 g          824 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcatac ttcgccgatt gtaaaattaa
     2041 gctagacaac taaaaaaagt catttgtgct acactcaaaa tagttccgac gaaagaatta
     2101 taaggagtga gtacaaatga cctataacca tcttacacca actgaactcg taatgataga
     2161 agcttatttc aatcaatcac aacctgtttc aaaagtagcc aatctgttac aacgttctcg
     2221 acaaactatt tataaggtct atcgttttct taaatctggt ggaagcgcaa tgagctacta
     2281 taaacaatat aagaaaaaca aacgtaactg cggaagacat ccgattgttt tacctgatga
     2341 gcaacaggaa tacattcaaa aaaaggttgt tcaagggtgg gctcctgatg tccttattgg
     2401 tcgagcagag tttcctattg cttgttcggt acgcacactg tatcgaatgt ttaaagataa
     2461 aacctttgac actcacgatt taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga
     2521 aaaaagagga aaacaagggt ttcgccggtc aatccgagac agaaagacag actataccca
     2581 gtttgatcat gaatttggtc acctggaagg tgacactatc gtgggtggaa aacataaaag
     2641 cgcggtcatc acattagttg aacggctatc taaagtcatc attaccttga aacccgaagg
     2701 cagacaggct aaagacattg aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa
     2761 cttatttaaa tccatcacat ttgattgtgg aaaagaattc tcaaattgga aaaagattag
     2821 taataccaac gatatttcaa tttattttgc ggatccagga acaccttctc aacgaggatt
     2881 gaatgaacat tcaaatggct tattacgtaa agatggtttg tataaagcta tggattttaa
     2941 tggtgtatct gaaaccttta tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa
     3001 atcattacac tatcggacac caatggaagt atttttgagt tacgtcacaa aagatg
//
LOCUS       DAEGIQ010000196         2899 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000196 DAEGIQ010000000
VERSION     DAEGIQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.206"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            246..542
                     /locus_tag="KMT45_002848"
     CDS             246..542
                     /locus_tag="KMT45_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006266.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            544..966
                     /locus_tag="KMT45_002849"
     CDS             544..966
                     /locus_tag="KMT45_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006267.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            985..2577
                     /locus_tag="KMT45_002850"
     CDS             985..2577
                     /locus_tag="KMT45_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006268.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2598..>2899
                     /locus_tag="KMT45_002851"
     CDS             2598..>2899
                     /locus_tag="KMT45_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006269.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCK"
BASE COUNT         1162 a          428 c          571 g          738 t
ORIGIN      
        1 tggttatcga ttacacgtgc agcactcccc cccgaatttt aaagagcata aggaaacgtg
       61 accccacttt taaaaaaagt ggttggatag gctgtgccta tcacgtttct gaaaaaaata
      121 aatgacccca aaataccaat atataggata gtacaaggat agcaaaggat agtaaaagga
      181 tagaggtagg atagcgaaaa ggataggatc cttttcacta tcctatatta tattggagga
      241 tatttatgaa tgttaaaatt agagatttag atccaaaatt catttctgaa attgatcgaa
      301 gatgcgttga actaagtaat cgtacaggaa ataaatggag tcgtaatgat tatttaaagc
      361 tactcgttga aaatgatttt gatcgtcctt tgatggaata caaacaagaa aagtttgacc
      421 agttattaga aaagttttca gaaattcaaa gttataacac aaagatatta gaagaatatg
      481 ttagccaaaa taatcgtatt atagaaatct tgattgagca aaatagagga agtgaacttt
      541 gatatggcaa aggttataga caaacacatt tactttcacc aaacacatat cgatgtattg
      601 gatgaaacta tttctactca tgcagaggtt aaaaactata gtgaagctgt tcgcttttta
      661 tgtatgaact atgatccaga agtggagaaa aaagtaaatt ccaatcaagc aaagttgaat
      721 ggaatgagta aagaaatttc tattttaacc gaaatggttt cagaatttgc ggatcttcat
      781 ttgaaagata caggaatatt aattggtaaa aattcaactg tttatcaaga agcaaaagaa
      841 cgagtggaag gaaaaattaa atccaatcaa acaaaaaaat attcccctca gttgaagaaa
      901 cctgtgaatg aatctgttca tgccagagta gatccatttg atccaacgaa gattctaaaa
      961 agataaaaat gaaggaggat aataatgact caatcgcctt cagttattct cacttgtcaa
     1021 tttactttgc cgaataaaaa gtcattttct acttatattg actatattac aagggaacag
     1081 gctcttgaag aaaaggaacg acgtacgccc gaagaagaac aggaacttca acgaatcaag
     1141 aatgctttag aggagtttta tattcctgaa ggagaaactt tttcggagag taagacgggt
     1201 aaagaattca gtgaaacgtc gaatgaagct caatcgttga tggaacatgg aatcaatttt
     1261 gacaagttag aggaacaaga ttttaccaat tatctttctt atatgacacg tcactatgcg
     1321 ttagaacaaa agaaagaact gacgcaaagt gaacaaaaag aataccaacg cttgaaccaa
     1381 gcaatgaaaa aatatcaaac agaaccccat aaaaaagctt caggaaatct tcctggagtt
     1441 ttttctatag cttcagacga agtgaaagga agtgatctga aggagattaa gcaaatcttc
     1501 caaagaggac aacaaaaagg ctcaatcatt tatcaagatg ttgtttctca tgatcattcg
     1561 tatcttgaaa aaataggatt gtatgatccc aaaacagata ctttagatga agaagggtta
     1621 aaagcagctg gaagaaaaat gatggagaca ttattcgaga aagaacaatt gaatgaaaca
     1681 ggttactgga tggcaacgat tcaccgtaac actaaacata tccatattca ttttgcagtt
     1741 gttgaaaaag aaaatacgcg taaaccttac acggaggttg aaaatggtgt gagttatatc
     1801 gtaccgaaag gtaagagaag acaagcaaca ttagacgcaa tgaagaccaa ttatgtacat
     1861 gagttagaac gtttttctta tgaaaaggat cgaatgattg gtatggagaa acgaaattta
     1921 ttgacgcgga aaagtgattt gcgtaacacc ttaacaaaag tggtaaaaga gccagcgcgt
     1981 tacgatcgac acgcgatgaa cttattaaat gaagtatata aaaccctacc tgacaaacgt
     2041 tcggaatgga attacgggga tgaaaaacga accaaacttt caccatacac aagagagaaa
     2101 ctcgatgagt taaccaaata tgttttaaca aaagaacctg aatatcaaga atatatacag
     2161 atttcaaaag cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca
     2221 aaggatgcag agaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccatccta
     2281 acagagctga agagacagga tagtcaaata aaaccattaa tagaaagtgt tgatcagatg
     2341 caatctattt acaatgaacg atcctttcga acgcttaaac aattgccaac gaatgaatac
     2401 aaatatagtt acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg
     2461 cagcggagtt tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca
     2521 agtaaagcat acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaaaga
     2581 cagtaggagg aatagcaatg aaacaatatt tagatcaatg gaaagttata gaaggatcat
     2641 taagagaaga acgaatcgaa caacttcctg attgtttaga aaaagagcat ttgttccaga
     2701 taagagaaat gttgagaaac gaacagtttg atccaaatca atttttagtc gttgagtacc
     2761 cagctactgg tgtgtattgt tgtaatcatg tgaatggtga aaaatatttt atcattcaag
     2821 aatatgaggg caagttagca ccttattata ctacttggga aatgaatgaa gagggaatca
     2881 ataactttcc atgtaaaag
//
LOCUS       DAEGIQ010000197         2852 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000197 DAEGIQ010000000
VERSION     DAEGIQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.207"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(116..478)
                     /locus_tag="KMT45_002852"
     CDS             complement(116..478)
                     /locus_tag="KMT45_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006270.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(552..932)
                     /locus_tag="KMT45_002853"
     CDS             complement(552..932)
                     /locus_tag="KMT45_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBE7006271.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            996..2189
                     /locus_tag="KMT45_002854"
     CDS             996..2189
                     /locus_tag="KMT45_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBE7006272.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2266..2850)
                     /locus_tag="KMT45_002855"
     CDS             complement(2266..2850)
                     /locus_tag="KMT45_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE7006273.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          886 a          554 c          481 g          931 t
ORIGIN      
        1 taaaatgcac taaaaaacta tctttagcag aacatttcat tgtatttttt ggaccaaaca
       61 ataaaaaaag aagtttgaaa aaaattcaaa cttctttgtt cgcttaatct gtattttaga
      121 agaaaggcac tccctctact tcgtaagttc caagaacaga gcgcatttga cctggttctt
      181 cccaaatata tccttcgcct aattcttctc tttgattctt ttgttcagtt tcaaagacaa
      241 acaacggaac attttcctct ttaatatgtg tatttgtcaa ttccatcaaa ctaatcttgc
      301 taacatcgat atgaactatt tcttttaaga aattaagtat attcgctaaa ccagtctttc
      361 cttcagaaag ttccgctaac gcaaattcca acttgtcatc aattgaatgg accaaaaatt
      421 ttttagtacc atcctccaaa tgcaacataa ttgtaccagc aactcttttg cttgccaaaa
      481 tttccacctc gatcaacttg ttctatggct tacataaaaa ttctagcaca ttttcagaaa
      541 aaatacactt tttatttctt attttctttc cgttctagtt cagataatgc ttcttctatc
      601 caaataggta actgctccgc aatagtggta aatccgatct tacgattttt attcgtgaaa
      661 tacctttttc tgcgatatgg aacagctgga tttgtttttt ctaacgttct taaagataaa
      721 ctgatttttt tggaatattc atcaatgtcg atgatctgaa ctgttactgg ctgtccgact
      781 tttaacaatg aatggatatt tttcgtataa ccggattgta cttctgaaac atgaataagt
      841 ccttgcgtct cgttatcaag agaaacgaat gcgccataag gctgtatccc tgtcactttt
      901 ccttcaatta tttgtccaat tttataagtc atctacttcc ctctcttcgc atttatcatt
      961 atactgtaaa tagctcaaaa aaagaagcga cgaaaatgac tggaggtctt aagatgatcg
     1021 aatttgataa acagatttca agaaaagata cagaaagtgt aaaatgggac gcaattgctg
     1081 agacatatca aatggatgac ttacttcctt tgtgggtagc agatatggac ttcttgtctc
     1141 ccggtggggt agctaaagcg tttagtgaat acatcaaaca tggaatcttt ggttatgcca
     1201 ctctgtctga taaactatat caggctatca ttcattggga acgccaacac catgctgtgg
     1261 agttgacgaa agaaaatatc gtttttacaa gtggtgtgtt aaccagttta gctgcagcag
     1321 tacaaacttt tacaaaacca ggcgattcta ttttgattca tgacccagtt tatcctccat
     1381 tttcctctat tatagaaaca aatcaacgtc acatcgttcg aagctctttg ctagaaaaaa
     1441 ataatcattt catcatggac ttgtctgaca tggagaaaaa aatcgaagaa aacaatgtaa
     1501 aagcaatgat tttatgcaat cctcacaatc ctggagggcg tgtttggagc aaagaagaat
     1561 tgaaacagtt aagcgaattg tgtttaaaac atcaagtgat tctgtttagt gacgagatcc
     1621 atcaagattt aactttattc gatcatacat ttacttccat gctaacgatt gatcctaatt
     1681 tagatgatct actagttgct tttacttccg caaccaagac tttcaatcta gctgcaatca
     1741 aaaattcaat ggtatttatc aaaaatccag agatgaaagc tgattttgaa aaacatttag
     1801 tgatgaacca acagcacgaa atcaacactt ttggactgat tggaacccaa gcggcttatg
     1861 agacgggtgg agaatggtta agccaactga ttccttatct agaaaaaaat gttcaaacag
     1921 ttaatcagtt ttttgaacag tatcttccga aagtgagaat aatgaaccct gaaggaactt
     1981 acttaatgtg gttagatttt accgcctata tttcagacga ccaagaattg gagaacactc
     2041 ttgttcggaa aggaaaagtc gtattaaatc ctggcatcac gtttggtcct tccggtcatg
     2101 ggcatatgcg cttgaatatt gcttgtccag aagaaacttt actggaagga cttaatcgaa
     2161 tcaagtatgc tttagagaca atcgattaaa tcatttattc aaaataaaag gacgagacct
     2221 tactaggttt cgtccttttt cataattact ccgtaccata ccaaactatt ctgaaatttc
     2281 aattgtttca atcactacat catgtacagg tcggtcttgt ggaccacgtt gtacatttgc
     2341 gatttcatct actgtgtcca tcccttctag tacatgtcca aagactgtat gtcggaaatc
     2401 tagccaagga gttccgccac ctttatacgc ttcaatgatt tctgatggga atccagcacc
     2461 ttctagttgt ccaagcatat tagctggcac atttgtgtta tttacaataa agaattggct
     2521 tccgtttgtg tttggacctg aatttgccat agataaagct ccgcgaaggt tgaacagttc
     2581 acgactgaat tcatcttcaa aactttcgcc ataaatactt tcgccaccca ttcctgttcc
     2641 agttggatcg ccgccttgga tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc
     2701 ataataacct tttttggaaa gttcaatgaa attttgaacc gtcttcggtg ctagttcagg
     2761 gaataattga acagtaatgt caccacggtt tgttttgata acagcttttg gtcctttttc
     2821 actttttaaa tctagttgtg ggaatgccat at
//
LOCUS       DAEGIQ010000198         2801 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000198 DAEGIQ010000000
VERSION     DAEGIQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.209"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            139..1362
                     /locus_tag="KMT45_002856"
     CDS             139..1362
                     /locus_tag="KMT45_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBE7006274.1"
                     /translation="MESYQFQIFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            1397..2164
                     /locus_tag="KMT45_002857"
     CDS             1397..2164
                     /locus_tag="KMT45_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBE7006275.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            2356..2754
                     /locus_tag="KMT45_002858"
     CDS             2356..2754
                     /locus_tag="KMT45_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBE7006276.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT         1049 a          394 c          473 g          885 t
ORIGIN      
        1 taaaatagcg atttctgctg tcttatcaat gaaaattgat taaaaaaata aaatgtgttt
       61 tacagagatt gagttatgtt ataaatttac gatacatttc ttattgtatc gtatcgaaaa
      121 caggtctata cttattcaat ggaatcgtac cagttccaaa tttttaagct cgtaccaagc
      181 ttagaaagaa gaggcaatcg tatgaaaata acagaaaaga atgtaaaccc gaaatttgaa
      241 cctaccgaaa gcaatctaaa aaagtttgat ttaactgtta ctttatcgaa tcaacttgta
      301 gatagcattg ttgaaatgga tgctatgcca caaaaaattt atctttatgc tataggacat
      361 cttgacccta aaaatccgcc ttttaataat actgttaaag tatctaaaaa ggctttattt
      421 gcgttgatgt cttctagttc atcttcttca aaacattcaa gattaaaaca agttattgaa
      481 aaattgcatc agcaatctgt cattaaaatt caaagaacta aacctaacgg taagactgaa
      541 tacaaagtca tctcaccgct agaaattact gaattcaatg atttttctga tgcagtatct
      601 attaaattta gtagttcaat tatgccttta ttaacgaact tagaagatca ttttacgcaa
      661 tatacatttg atcaagtagt taacttgaca agcaagtatg cgatcagttt ttttcaatta
      721 ttcactaaag attttaatct gtatgaagca aatagagata acttaacatg gagtacaaaa
      781 tcacttgata gattaaaaaa tcctgttttc tccattgcag atttacgaaa gaaatttagt
      841 attgagaaaa aatataaagc ttttcgagac tttgaaagac gtgtaattaa agaaccgatt
      901 gcccaactga ataaatacac tgattttaaa attgactatg agcttatcaa gaagggtcat
      961 acttatacaa acataaagtt cttgcttaca cgtaccatgt cagttccggt accttataag
     1021 caagaacaaa gagaccctgc ttatttaaaa ggcgcagaag ataaaacaga gaaaatgaat
     1081 caatcgtact tgaaagctat gaatagcaaa tacactaaat tactaatcaa aaactttttg
     1141 atcagttctg ccgacttatg cgatcttaaa cttatgagcc gacttcagaa taaagtttat
     1201 ccgctttatg atcgtttatc caaagataga ggatttgaat cagttgtcaa tcacctatct
     1261 tacgttgcta ctcataagga ggattacaca ataaaaaata ctgttaaata tctcgaaaaa
     1321 tcaattacga gttatttgcc ttatgtgaac ctttatgatt aacaactgaa aaaatgcgtg
     1381 aaggaaggaa aaacttttga aaaaagatga actgacaaag tacaatgagg agttcttagc
     1441 aacaattgac atgaaaatgg atttaaagtc gtatgaactt tatcaattgc tgttcagtta
     1501 tatctcaccc acaaagctaa agaaaacagg cgtagtagat ttacctgtta aagcgattat
     1561 taattacatg gatgttgatc aaaatcttga aacggaacta aaaaccatag ttaacacttt
     1621 gcaaagacaa gcagtttatg aaatttcgaa tctagacaaa aaatataaga cgtatactac
     1681 tgtatctgct attgatcgga ttcattggga tttagaagag catagtgtat ctttcagttt
     1741 ttcagaagat gtaattccct taattgtctc aatcaaaaag aagtatccgt tctttgtttt
     1801 tgaagactta gcatcttttc aaaaaaaata tagcatcgtg ttatacaaat atttggcgat
     1861 gtattataaa aaatatctta tcgccaaaaa taaaggagat tttgagaaag ctgaaatata
     1921 taagaatccc ctgattgaaa tagttaaaat tcgtgagatt acgaaaacag aaaatgagta
     1981 taaaacaata tcgaattttg atagacgtgt tttaagggaa tcagttgagg aaattaatga
     2041 aaaatcaatg ctgaatattg attttgctaa agttagaaaa ggcagaaatt acaattttgc
     2101 tgtccagttt aatatttcta gtgagtttga tgaaaaaact tatagagaaa aaaaattagg
     2161 ttaatgcaaa atatatctta ctcgtttgaa gatgcttcag acgtatgcta agatatatag
     2221 tgccttggga gcaaggtatt aatctccttt agaggttaat tagcttgtga tcgcctatca
     2281 atttttttgg taggcgattc ttttttttga aacaaaaaag caaaaattat tttttaatta
     2341 gagaggtgca atgcgatgaa atactttttt tgtgcagtat tcgaatggaa aggaaatgaa
     2401 tatgcagtta aattcccaga ttttccagac ttatatacac atggtgaaac tttagaagat
     2461 gcttttgaga tggcgcaaga agtattggaa ggatacttgg tgcttttaga gttgcaagaa
     2521 aaagaaattc catcgcctac gcctgttcaa gagttaaatg caagaatcaa gcgatctgag
     2581 aatgttaatt tatttgtcgt aaaagctaat acgaaagttg ctaaagctcg aaacaagcaa
     2641 aattatgatc gaaaatcagt gactgtggaa cactatttaa atgttttagc ggctgaagag
     2701 aatattaatc tgtcgaaagc tttgaatgat ttgttgagag aaaagttgat ttgatgtctc
     2761 tcagatgatc atattaagca aaaacagctt gttttaggac g
//
LOCUS       DAEGIQ010000199         2751 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000199 DAEGIQ010000000
VERSION     DAEGIQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.210"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            50..1438
                     /locus_tag="KMT45_002859"
     CDS             50..1438
                     /locus_tag="KMT45_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBE7006277.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>2751
                     /locus_tag="KMT45_002860"
     CDS             1776..>2751
                     /locus_tag="KMT45_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBE7006278.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFS"
BASE COUNT         1034 a          414 c          486 g          817 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct g
//
LOCUS       DAEGIQ010000200         2635 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000200 DAEGIQ010000000
VERSION     DAEGIQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.211"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            136..1431
                     /locus_tag="KMT45_002861"
     CDS             136..1431
                     /locus_tag="KMT45_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBE7006279.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIKVR"
     gene            complement(1586..1657)
                     /locus_tag="KMT45_002862"
     tRNA            complement(1586..1657)
                     /locus_tag="KMT45_002862"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1622..1624),aa:Glu,seq:ttc)
     gene            complement(1669..1742)
                     /locus_tag="KMT45_002863"
     tRNA            complement(1669..1742)
                     /locus_tag="KMT45_002863"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1706..1708),aa:Ile,seq:gat)
     gene            complement(1755..1825)
                     /locus_tag="KMT45_002864"
     tRNA            complement(1755..1825)
                     /locus_tag="KMT45_002864"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1791..1793),aa:Gly,seq:tcc)
     gene            complement(1830..1902)
                     /locus_tag="KMT45_002865"
     tRNA            complement(1830..1902)
                     /locus_tag="KMT45_002865"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1867..1869),aa:Phe,seq:gaa)
     gene            complement(1906..1978)
                     /locus_tag="KMT45_002866"
     tRNA            complement(1906..1978)
                     /locus_tag="KMT45_002866"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1943..1945),aa:Asp,seq:gtc)
     gene            complement(1997..2070)
                     /locus_tag="KMT45_002867"
     tRNA            complement(1997..2070)
                     /locus_tag="KMT45_002867"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2034..2036),aa:Met,seq:cat)
     gene            complement(2083..2172)
                     /locus_tag="KMT45_002868"
     tRNA            complement(2083..2172)
                     /locus_tag="KMT45_002868"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2134..2136),aa:Ser,seq:tga)
     gene            complement(2195..2266)
                     /locus_tag="KMT45_002869"
     tRNA            complement(2195..2266)
                     /locus_tag="KMT45_002869"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2231..2233),aa:Glu,seq:ttc)
     gene            complement(2288..2375)
                     /locus_tag="KMT45_002870"
     tRNA            complement(2288..2375)
                     /locus_tag="KMT45_002870"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2339..2341),aa:Ser,seq:gct)
     gene            complement(2400..2473)
                     /locus_tag="KMT45_002871"
     tRNA            complement(2400..2473)
                     /locus_tag="KMT45_002871"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2437..2439),aa:Ile,seq:gat)
     gene            complement(2487..2557)
                     /locus_tag="KMT45_002872"
     tRNA            complement(2487..2557)
                     /locus_tag="KMT45_002872"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2523..2525),aa:Gly,seq:tcc)
     gene            complement(2559..2634)
                     /locus_tag="KMT45_002873"
     tRNA            complement(2559..2634)
                     /locus_tag="KMT45_002873"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2599..2601),aa:Asp,seq:gtc)
BASE COUNT          833 a          589 c          543 g          670 t
ORIGIN      
        1 gctctataat ttatgggact gatgaatcag aattgattct tcagccccat ttttatttta
       61 ggtattaaaa aacatatcgt tctctagtat gattaaatca cgacaaaata aacaactgag
      121 aggacgatat gctatatgaa tgattctatc aaaaaaatgc tgcgattaat agagaaagat
      181 ttgatgatta cagaggtctc ttacgagacc tttcagaaga aaaagacgtt gatcgtcgat
      241 gctgttttct cacctgctcc ccatacctgt agaaattgtg gttctactgt ggtagatgga
      301 aacgggaaag taattgtagt gaaaaatggg aagaaggaaa cgattgtccg ctttgaacaa
      361 tacaatcaca tgcctttggt tatgcgctta aaaaagcagc gctatacctg taaaaactgt
      421 cgaacccatt ggacggctca aagttatttt gttcaaccca gacattcaat cgcgaatcat
      481 gtcagatata aaattgcttc tttactgact gaaaaagtat ctttatcttt tattgcgaaa
      541 aattgtcagg tatctttgac caccgttatt cgtacgttga aagagtttaa aagctactta
      601 ccaaagcaat ctaagaggat tctccccaga gtattgatgg ttgatgaatt tcgttcgcac
      661 gcttccatag aagataaaat gagctttatt tgcgcagatg gcgaaacagg acaattaatt
      721 gatgtcttgc ctacgcgtaa attgcctcga ttaacaagct atttcttagg ctgcgctaat
      781 cctgaagaag taaaattttt agtcacagac atgaacgccg cctacttcca gcttaccaaa
      841 cgtattctgc caaatgcgaa agtagtgatt gatcggtttc atattatcaa acacatgaat
      901 caagccttta atgagttgcg tatccgtgaa atgaatgaac ttcgtaaagt aggacagaaa
      961 agtcaggcag aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc
     1021 aaccattatg aatacaaaac atggaaaagt tttcgagcac caaaataccc atttcttacg
     1081 gaagcgatga tgattgatcg attgcttggc ttttctacgt ccctgaagga ggcgtatccc
     1141 tattttcatg aattagttga agcctttcga gacaaagacc ctgacttatt tttctcctta
     1201 ttggcagaac ttcccgaaac gttagatgac ggctttcggg aaaagctcca aaaccttctg
     1261 acctatgaag aaggcatcac caacgcgatg atctatcctt acaccaatgg aaaaatagaa
     1321 gcgaagaata cccatataaa gacaatgaaa cgagtatcct acggatttaa atcatttgag
     1381 aacatgagaa ttagaatctt tttgatcaat caattaatca aagtaagata acaaaaaatc
     1441 tgagccagaa tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta
     1501 ttgacaaaga gccgataact ctgctaagct ttttaaaatc attctttcag ataaaaagaa
     1561 aaagatactc aaaaagtatc ttccatgacc cgtacgggat tcgaacccgt gttaccgccg
     1621 tgaaagggcg gtgtcttaac cgcttgacca acgggccaat aaaataaatg ggcctaaatg
     1681 gactcgaacc atcgacctca cgcttatcag gcgtgcgctc taaccagctg agctataggc
     1741 ccataaaaac taaaagcggg tgacgagaat cgaactcgcg acaacagctt ggaaggctgt
     1801 ggttttacca ctaaactaca cccgcataat ggcgcgggac agaatcgaac tgccgacaca
     1861 tggagcttca atccattgct ctaccaactg agctaccgag ccaaacggtt ccgacgggaa
     1921 tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa cccctacacc acggaaccaa
     1981 tgtttttagt ttttaattgc gggagcagga tttgaaccta cgaccttcgg gttatgagcc
     2041 cgacgagcta ccagactgct ccatcccgcg ataatatgaa aaaggaggat aagggattcg
     2101 aacccttgca cggttttacc cgcctgacgg ttttcaagac cgttcccttc agccggactt
     2161 gggtaatcct ccatgtaaaa ctatattaaa ttaatgaccc gtacgggatt cgaacccgtg
     2221 ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt aaaacaaaat
     2281 attgaaacgg agaaggaggg atttgaaccc tcgcgccggt ttcccgacct acacccttag
     2341 caggggcgcc tcttcagcca cttgagtact tccccaaaac caataagatt ttgtactaat
     2401 gggcctaaat ggactcgaac catcgacctc acgcttatca ggcgtgcgct ctaaccagct
     2461 gagctatagg cccataaaaa actaaaagcg ggtgacgaga atcgaactcg cgacaacagc
     2521 ttggaaggct gtggttttac cactaaacta cacccgcatg gcggttccga cgggaatcga
     2581 acccgcgatc tcctgcgtga caggcaggca tgttaacccc tacaccacgg aacca
//
LOCUS       DAEGIQ010000201         2615 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000201 DAEGIQ010000000
VERSION     DAEGIQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.212"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(39..848)
                     /locus_tag="KMT45_002874"
     CDS             complement(39..848)
                     /locus_tag="KMT45_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBE7006280.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1141..2577
                     /locus_tag="KMT45_002875"
     CDS             1141..2577
                     /locus_tag="KMT45_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBE7006281.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT          740 a          472 c          527 g          876 t
ORIGIN      
        1 attgtcacaa cctctttttt gtggaatcta attctctatt atagtttaag tagtttttcc
       61 agataaacag ctaaaccgtc ttcctgattg ctcaaaggag tgacatcatt tgcaatgcct
      121 ttgagttggt cagtaccgtt agccatagct actccccaac ctgcataatc caacaattcc
      181 aaatcattat gctcgtcacc aaaggcaatg acgtcttttt gatcgataga caggtagtta
      241 gcaatctctt gcacgccttt tgctttttgg attccttttg aaacaatctc taagatggca
      301 gtaggtccac cccatgtccg aacatcgaca taactgccaa attgacgtgt taattcagca
      361 gatacagctc ctgcaaaacg tttgtctgtt cggacaagca aagatgtagg atttgtcgtc
      421 aaatttttag gagaaagcaa attcttctct cctggacgag aagaagcaaa aattttttca
      481 tcaaagaaat caaaactgtc aataaaaaaa gtatcacgat tttcagcagc gataaaatct
      541 aaatttagct gctttttttg cgataagatt tcaaaagcga tttcacgatt gatcaacgtt
      601 tctctttcac catcccagtg ctgattaggg atgtgtacta atgcgccgtt aaaattaacc
      661 atcggtgttg ttaattctaa ttgttggtaa aactgatggc tcattcggaa tgggcgtcct
      721 gtagcaatac tgacatgatg accggcattg attgcttttt ttagagtctc ttttgtttta
      781 gatgtaatca tcgaatcttg attgagggta gttccgtcca gatcgatggc aattaatttt
      841 ttattcaata agggtccttc cttcctcaac tagcatttgt tttagtctag catattttag
      901 gtaattaaca agagcaatca gctattccta acaaaaacta cagttatggc tatataaata
      961 gctaaacata agctaaacac gaacgttaag tattattttc gctttttttg ttaaaaaaac
     1021 gttgaaaagc gaatgaaacg tctatataat ttgaaattgt tcgatggatt tgccctattt
     1081 tacttatttt ttattattat agttttccat cagaaaaaaa ttcagggagt ggaacttttt
     1141 atggaaaagt ttttcggttt aaagaaaaac aatacaaatg tatcaactga aataatggcg
     1201 ggagtaacga cattctttgc catgagttat atcttattcg taaatccgac gattttgtca
     1261 gcatcaggta tgcccttcca agcagtattt ctagcgacaa tcattgcatc gattattggt
     1321 acgctggtaa tgggattgtt cgcaaatgta ccatatgctc aggcacctgg tatgggattg
     1381 aacgcatttt tcacttttac ggtcgttttt gggcttggtt attcttggca acaagccttg
     1441 gcaatggtgt ttatttgcgg attaatcaat atttttatta ctgtcacaaa tattcgaaaa
     1501 atgattattc gggcgattcc cgaaagtctt caacatgcga ttggtggcgg gatcggtatt
     1561 ttcgttgcgt atgtcggaat caaaaatgca ggattcttat ctttttcagc agaccagtca
     1621 gctatttcga gctctgtcgt tgaaggcgga aaagcgacaa atgttacgat aaatggtgga
     1681 atcgtccctg cgttagccaa ctttgacaac gctcctatct tattggctgt gattggtttg
     1741 gtactgacag ccattttagt tgtaaaaaat gtccgtgggg ctattcttat cggtatcgta
     1801 gcgaccacag tccttggtat ttttatgggt gttgttgatt taagttccat tgattggcaa
     1861 acgaattcgt taggtaattc atttaaagaa ttaggaacaa ctttcggtgc agcatttggt
     1921 tctgaaggca tgcaatcttt attcagtgat tcttccaaga ttcctcaagt attgatgacg
     1981 atcatcgcat tcagtttatc tgatactttc gatacgatcg gaacatttat cggtacagga
     2041 cgccgcacag gtattttctc aaaagaagac gagatcgcgt tggaagatgg tcgtgggttc
     2101 aaaacaaaaa tggataaagc actatttgcc gatgcgatcg ctacttctgt gggggctatc
     2161 tttggtacat cgaatacaac aacttatgtc gaatctgcag ctgggatcgg ggccggtgga
     2221 cgtactggtt tgacttcagt agtagtggca gtgctattcg cattaagtag tttattctct
     2281 ccactgatcg ccattgtacc aaatcaagca actgcgccag cattgatttt agttggagta
     2341 atgatgatgg cttcttttgc agacatcaaa tggctagata tggaagaagc tttaccggcc
     2401 ttctttgcat cgatctttat gggattgtgc tatagcattt cctatgggat tgcagcagga
     2461 tttattttct atacaattgt gaaagtaatc aaaggaaaat acaatcaagt ttctgttgct
     2521 ttgtggatcg ttgatatttt attcattatc aacttcatca ttctagcaac aatttaacta
     2581 aagagaaggg attggaacag tatttgttct gatct
//
LOCUS       DAEGIQ010000202         2547 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000202 DAEGIQ010000000
VERSION     DAEGIQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.213"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(45..1022)
                     /locus_tag="KMT45_002876"
     CDS             complement(45..1022)
                     /locus_tag="KMT45_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBE7006282.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="KMT45_002877"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="KMT45_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBE7006283.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
BASE COUNT          693 a          532 c          473 g          849 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaatattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaa
//
LOCUS       DAEGIQ010000203         2531 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000203 DAEGIQ010000000
VERSION     DAEGIQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.214"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..345
                     /locus_tag="KMT45_002878"
     CDS             <1..345
                     /locus_tag="KMT45_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBE7006284.1"
                     /translation="LLVTSEGAFKYFSRAYDLPAAYIWEINTESQGTPDQMKAIIDQI
                     RAKEVPVLFVETSVDSRSMERVAKETGLKIYDKLFTDSIAKEGEQGDSYYQMMKWNIE
                     TIHEGLSQTKES"
     gene            486..1238
                     /locus_tag="KMT45_002879"
     CDS             486..1238
                     /locus_tag="KMT45_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006285.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            1257..2408
                     /gene="queG"
                     /locus_tag="KMT45_002880"
     CDS             1257..2408
                     /gene="queG"
                     /locus_tag="KMT45_002880"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBE7006286.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT          891 a          405 c          544 g          691 t
ORIGIN      
        1 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
       61 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
      121 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
      181 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
      241 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
      301 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
      361 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
      421 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
      481 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
      541 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
      601 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
      661 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
      721 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
      781 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
      841 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
      901 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
      961 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
     1021 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
     1081 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
     1141 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
     1201 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
     1261 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
     1321 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
     1381 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
     1441 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
     1501 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
     1561 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
     1621 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
     1681 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
     1741 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
     1801 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
     1861 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
     1921 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
     1981 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
     2041 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
     2101 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
     2161 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
     2221 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
     2281 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
     2341 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
     2401 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
     2461 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
     2521 tttgcttttt t
//
LOCUS       DAEGIQ010000204         2488 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000204 DAEGIQ010000000
VERSION     DAEGIQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.215"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1..296)
                     /locus_tag="KMT45_002881"
                     /pseudo
     CDS             complement(1..296)
                     /locus_tag="KMT45_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(556..1104)
                     /locus_tag="KMT45_002882"
     CDS             complement(556..1104)
                     /locus_tag="KMT45_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006287.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1105..1962)
                     /locus_tag="KMT45_002883"
     CDS             complement(1105..1962)
                     /locus_tag="KMT45_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBE7006288.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2248..2469)
                     /locus_tag="KMT45_002884"
     CDS             complement(2248..2469)
                     /locus_tag="KMT45_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE7006289.1"
                     /translation="MKEKFLNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRV
                     AYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          717 a          511 c          323 g          937 t
ORIGIN      
        1 ttgatctcgc ttattacaat cgctttcatt tgtcctaaga gtcaaatctt tttccaccat
       61 aacttttaca cccaataaat tttgcggttt atttttctct gttgaaattc tttgtgtagc
      121 ctttaaactg acaattagct cattgggtac atacttattt gagagtcatc taattggctc
      181 ataatccttt ctttttggtt agtatctgca gttacttgtt tagtatgagc catgaaccca
      241 aacatactca ccccaacaac caatcctaaa tatattttct ttttaaactt tttcacctac
      301 atctcctcct aatagtttta ttttagagtt agctctatta cttatttact aactcaacaa
      361 cagattatca tacacttgtt aatgaaatca caatatctca ttaaaacaca ccctaaaatc
      421 ataaccgttc ctatgctcat tttttggatt ttttaatcac gtaaccagaa aaataaatcc
      481 cctctatttc atgcaaatca aagaagaact aagtaactac cgtttaaagg tagttactta
      541 gttcttcttt gattattatc taactttatt attcattccc caccctttgc gcagtgagtc
      601 ttcggtatct tgttttatct gactggtagt tcttattatt tctccacgat catctaattc
      661 tggaacaatt ggatattttt ctctgaacaa gtctagttct ttcaccacgt cattaaacaa
      721 atcttgctta tcttcttgcg taagattctg tttaatatac tcatctaaag cttttctaag
      781 caattcatct cttgtcatat tagtctcata cttttctact aaaactctgg aagtcgcatt
      841 taacaaagtc gacagtttta aaggatgctt ggtggaataa accttatcaa actttctgat
      901 tggtgcgcca actttttttg ttcttcctct actctgtctt tcaacattgg aggaagtttt
      961 tatttcttga acttggtgca cttctaattg cttattttca gatggtgtaa ttgcttgctg
     1021 ctttttcttt gcttctgctt ctttttttat tttggcgagt cctttgttgt tcccaaaaat
     1081 atcaagtcct ttgatgtcag ccaattactc cacctccgca taccaattct cgtgttcttc
     1141 caattctttc aaaatactga taaataattc gtggcttttg cgatcccatt gttttaagag
     1201 acctttcttt gtcttctcca tcgtaatacc tgatccgtcg tatcgtttta agcgcgcatt
     1261 tttgagtaca acatttttaa tcaaatgttc cccataaatc tcttgggctt gtttatacat
     1321 ttcctgatct actgcgtcac cgctttccaa catgaaagga ataacaccag caacatataa
     1381 atttgtatca aattctttca caaaaaagtc catatactca agatattgca tcgcatttct
     1441 catcgatttt acttgggtct gtaagacgac gattgcccaa tccgcaatgt acatcgcgga
     1501 tttactatat acagaaatag aaggtgggac atctaaatag atgacgtcaa actcatcaaa
     1561 taatggttct agcttttctt tcatatattt gaattgggat aatgaatcat tatattggtc
     1621 tttgatccaa tcatcataca tttcaaagga catgtctgct ggaagtatat agaggttatt
     1681 gattacttta tagacagcct ctttaggtgg aagattgttt atcaacacgt cagtcacagt
     1741 attttcgtaa acaatcggtt caccttctcc gcctccttct tcgttagtga agttcatatg
     1801 cgttaagttc atcacatcag aagtatttcc ttgcatatcc aagtcaatta tcaaggttct
     1861 ttttccttca ttaactgctg attcaaaccc taacatttgc gcgcacgtac ttttacctac
     1921 gccaccttta aaattaccga ttacatattt cttcgctacc acgttcctac ctcttttctt
     1981 ttagcaatat tcatttgata ctattataac atatttattt ttagttacta ccaaaaaaaa
     2041 gatatatatg tactacctta taattaataa gtaaaaggta gtacataaat aaggaaaagc
     2101 tgaaatatat tgatttatag ctttcctcaa agtaatgacg ttatttaatt tatcgagaag
     2161 cagagaatac tcgcgacttt agtcatgtag acgaatcaga ttttcgataa aaaagaagcc
     2221 gtccttctta agaacgacaa caaatcacta gattaatttt ttcaactgtt cataaaagtt
     2281 ttcatgcgtt ccacataaca atactggaat aaccttgttt tctataatct cgtaagctac
     2341 tctatatgtt gtgcccgcat atttgaaccc actagaccaa atacctgata aatccccagt
     2401 tttttgttca cccttatatg gatcaatcgc aatttcatca taaattaaat tcaaaaattt
     2461 ttctttcaat ggcttctctt ttagcttt
//
LOCUS       DAEGIQ010000205         2467 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000205 DAEGIQ010000000
VERSION     DAEGIQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.216"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            81..>2467
                     /gene="esp"
                     /locus_tag="KMT45_002885"
     CDS             81..>2467
                     /gene="esp"
                     /locus_tag="KMT45_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBE7006290.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT          893 a          386 c          506 g          682 t
ORIGIN      
        1 cctattctgt gaataatacg tatcagattt atcttttatt cttactagga aagtaattat
       61 taagggagtt gatttgaata atggttagca agaataataa gagagtattt ctagaaaaaa
      121 caaagaaacg agtattgaag tacagtatca aaaaattaag tgttggggtt gcatctgttt
      181 tgattggtgt tggtcttgtc ttgggaacaa ctgaactagt taaagcacaa gatgaaattt
      241 ctccaagcag cccacttgaa acagcgataa gctccgtaca agtaggtgac aaagtagctt
      301 cggggaatac ttttcaggag aatcctggtt atacgaaaaa ttataacttt tcggatttac
      361 aatttagtcc tcaagaacta actggagaca cactaaaagg caatacaatt ggatttgaag
      421 tttacggaaa acataatatt gcagcatcaa ctaaaaactg ggaaatccgt cttcaattag
      481 atgaacgatt agctaaatat gtagaaaaaa ttcaggtcga tccaaagaaa ggaattggat
      541 cttctagacg gacctttgta agaattaatg attcgctggg tagacctaca aatatttgga
      601 aggtcaatta tattcgtgca agtgatggat tatttgctgg ggcagaaaca accgatacgc
      661 aaaccgctcc taatggggta attacgtttg aaaagagcct agatgaaatt tttaaagaga
      721 taggcataga caatctcaag accgatcgtt taatgtatcg aatctacttg gtaagtcatc
      781 aagacgatga taaaattgta ccgggtattg atagtacggg ctacttttta acagattcag
      841 atgatttcta taatagctta gatgtatctg aaaataatcc tgaccagttt aaacatggat
      901 cagtaagtgc aaaatatgag aaacctaata ctcaaacaaa agatggttca ggatccacag
      961 gtgctaatgg agctataatc ttagatcata agttaacgaa aaattataat ttctcatatt
     1021 catcttccgc aaagggcacg ccttggtatg ctaattacaa aattgatgaa cgattagttc
     1081 catatgtagc gggtatacag atgcacatgg ttcaggctga caaagtaaca tatgatgttt
     1141 cgtttgaatc cggaacaaaa gtagctgatt tagcaatcga gcgacgggaa ggtcatgaaa
     1201 attatggcat aggttcaata actaataatg atttaactaa acttatcgat tttgctaatg
     1261 caagtccacg tccagtcgtt attcgatatg ttttacaatt gactaagcca ttagatgaaa
     1321 tcttagaaga tatgaaagca acagcacaag ttgaagaaaa taaaccattt ggtgaagatt
     1381 tcatctttga ttcttggttg tcggatacga ataaaaaatt aatccagaac acttatggaa
     1441 caggttatta ttatttgcaa gatattgatg gtgatggaaa ccctgacgat aaagaagaga
     1501 gcggagacac gaatccatat atcgggaaac ctgaattaga agaagtatat gatgttgaca
     1561 caacagttaa ggggaaagta ttcatccacg agttagcggg aacaggtcac aaagcccaac
     1621 ttgttgataa agaaggtact gtattagcag aaaaaactat cgctccaaat gaaaaagatg
     1681 gggctccaat ttcagatact gtagaatttg aatttacggg tgtagattca agtaaattaa
     1741 tcgcgaaaga tgaattaaaa atccaaatcg tttctccagg ttttgataaa ccagaagaag
     1801 gttcaaccgt tattaaggaa tcaccaaaag cggttgataa acaaaccgtg gtagttggat
     1861 ttaaaccaga tgctaaagaa tcaattcgga ataataaaaa cttacctgaa gatgcagagt
     1921 attcatggaa aacagagcct gatacttcta acgttactga tagtacgaaa ggtattgtaa
     1981 ctgttaagat cggaaatcga actttcgacg tggatgtaga gtttgctgta aaagcttctc
     2041 aagctatgga aaatgatgca acatacgtac ctataacaac aactccagaa acgacagttc
     2101 aaagtggaaa acctacattt gataaaccag atgttccttt agctaaagat gccttttcaa
     2161 tttcagatgt ctataataag tacttcggta atgcaagtgt tgatgcaaac actggtattg
     2221 ttacattcac tccagctaaa ggtgtaggag aatcggagcc gattactgga acaattccta
     2281 ttaaaattgt ttaccaagat ggttctgtag gcacgaccga tttagcagta actgtaagta
     2341 aagatattta tgaaaatcca ggagaaaaca ttcctgcagg ctaccacaaa gtaaccttca
     2401 ccgcagggga aggaacaagt attgaaagtg gaacaacagt ctttgcagtg aaagacggcg
     2461 taagctt
//
LOCUS       DAEGIQ010000206         2467 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000206 DAEGIQ010000000
VERSION     DAEGIQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.217"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..832
                     /locus_tag="KMT45_002886"
     CDS             <1..832
                     /locus_tag="KMT45_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006291.1"
                     /translation="KKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRI
                     LNATIRKNSSGKFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTS
                     KMAKKLKREQRKLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKL
                     STEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVD
                     KWFPSSQICSNCGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA
                     "
     gene            1070..1333
                     /locus_tag="KMT45_002887"
     CDS             1070..1333
                     /locus_tag="KMT45_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBE7006292.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            1327..1677
                     /locus_tag="KMT45_002888"
     CDS             1327..1677
                     /locus_tag="KMT45_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBE7006293.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1701..2315)
                     /locus_tag="KMT45_002889"
     CDS             complement(1701..2315)
                     /locus_tag="KMT45_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7006294.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT          871 a          392 c          455 g          749 t
ORIGIN      
        1 taaaaaacat cctatacaaa gttatacaac taaaaatgtg aataatagta tcgaaattag
       61 aaacaatttc gtaaaactac ctaagttagg atttgttaaa tttgcgaaaa gtcaagagcc
      121 caaagggcga atcttaaatg ctacgattcg aaaaaattcg agtggtaaat tttttgtttc
      181 catcctgtgt gaagaagaaa tagttgagtt tcctaaatct aattccgcta ttggaatcga
      241 ccttggtata actgattttg caatcctttc tgatggtcaa aagattgata acaataaatt
      301 cacgtcaaaa atggcaaaaa aactcaaacg tgaacaacgt aagttgtcaa aacgtgcatt
      361 actagctaaa aagaaaggcg tcaatctctt tgaagctaaa aattatcaaa aacaaaaacg
      421 aaaagtagca cgactacatg aaaaagtaat gaatcaacgc aatgactttt taaacaagtt
      481 gagcacagag attatcaaaa accacgatat tatctgtata gaagacttaa atacaaaagg
      541 tatgttgcgt aatcataaac tagcaaaaag catttctgat gtgtcttggt ctagttttgt
      601 gactaagtta caatacaaag ctaactggta cggtcggaaa atcatcaaag tagataaatg
      661 gtttccatct agtcaaatct gttcaaattg tggacacaaa gacggcaaga aatctctaga
      721 aattcgagaa tggacttgtc ctgtttgtca tgctcatcat gaccgagata tcaatgctag
      781 tatcaatatt ttgaccgaag gtctaagatt acatgtcatg ggattagctt agatccaatc
      841 atcaaaaacc gtaggaacta cggggatagc ttggtaaata agagaaaccg ctgtgagtaa
      901 agaaatacgc ttgcaagtat gctctattcc caagaatctc gtgactttag tcatgagagg
      961 ttcaaggatt catggaatgt taatctaatt taatagaaaa ggataattaa gtatatacgt
     1021 tttttcttat gtatgttata ctatgtatat acagaaagag gtgtctacta tggaaatgtt
     1081 acctataaaa aaatggggaa atagtaatgg ccttagattg ccaaagtata ttatggaata
     1141 tcttggaatt catacagaag acaaagtgaa gataatacaa gaagaagtaa acggtcagaa
     1201 gcggctaatt atcgaagcgg ctaatataga aaatgaatta acaattgaac agttatttga
     1261 aaattacact gatgaaagaa atcatgtgac gattcaaaat ttaggtgaag cagtaggtaa
     1321 tgaaaaatgg tgagaaaacc tagacaaggc gatattctat tattgaatac agcacctcga
     1381 tctggacacg aacagacagg taaaagacct tatattgttt taagtcatga tattattgcg
     1441 gattatagta acgttgttac agttgctcca atttctagta caactagaaa ctatccgcta
     1501 tatgtaaata taaactcaga atatgaaatg aagacaacag gaaaagtatt attagatcaa
     1561 ctaacgacaa ttgattatga agccagagag tgtatttttt tggaaaaagc gcatgatagt
     1621 ttagttgagg agttattgat gaaagttaga acagtttttc aaaaagtaag caaataaaat
     1681 agaaacagca gcatgatcaa ctattcgtga ttgaccatgc tgtctttttt tattctatat
     1741 acggtttgtc tcgtaacatt gtagtctttt gcaatcttag cgatcgcaac accttttttt
     1801 agatcctcaa cgatgttttt ataaacaaga cgacgttggg gatcttttgc attggcactg
     1861 tatagcttag gacgtccttt ataaacaccg ttcgcttttg caatcttaat gccttgtgcc
     1921 tgacgtcgtt tagattcggt acgttcttgt tcagcgatca tggccaaaat ttgaatgatc
     1981 aaatctttaa tgaatttatc caggagagga ttgccaattg cttcggccat gatcggtaga
     2041 cttgtgatga tcagctgcac atttttcttc ttcaagtaat tgactgaagc gatgatctcg
     2101 tcgtaatttc ttccaagtcg atcaatagct tccacaataa aaatatcttg atctcggacg
     2161 aaatccagtg cttcttgaaa aacaggtcga tgagtaatcg ttgcgccaga ttgtttctca
     2221 atgaaaattc tctctgcacc gaattttttc atttcttcaa tctgccgttg ttcattttga
     2281 tcgatggatg agacacgtat gtatgcaact ctcaaaattt ttacctactt tctgcaacta
     2341 tttaaaaaac accctaatca tacacgagaa aaccagattt ttcaatgtcg cggtcgtgtc
     2401 tagttagggt ataccttata tagacaaaaa ctaatgatgg aggaaatagt atgtgtcact
     2461 tttcttt
//
LOCUS       DAEGIQ010000207         2443 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000207 DAEGIQ010000000
VERSION     DAEGIQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.218"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1..492)
                     /locus_tag="KMT45_002890"
                     /pseudo
     CDS             complement(1..492)
                     /locus_tag="KMT45_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(698..2380)
                     /locus_tag="KMT45_002891"
     CDS             complement(698..2380)
                     /locus_tag="KMT45_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBE7006295.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
BASE COUNT          796 a          523 c          390 g          734 t
ORIGIN      
        1 cttcgagatg accaaattct ttagtcaggt gtggatagag actgttccgt tcatgaatcg
       61 ttctgcgaaa ggcctgtttt ccacgctttt cttgatgatt attcggttta cgtttcccct
      121 tcataggtaa cgactgtgtg tcaaaaaggc cttttttaaa catccgatag agtgtacgaa
      181 cagaacaatc aattggaaac tcgccacggc cgataatcac atcgggtgac cacccttgag
      241 ctaccttttc ttgaacatat gttgattgtt catcagataa aacggttgaa cgtcgtccac
      301 aacgagattt attttgtttg tattgacggt aataatctag cacttttttc cttgtttgag
      361 aaacgaataa acattataaa tggtttgtct agagcgattt aaactttgtg cgacaagagc
      421 gacaggtttc tcgatattga aataagactc tatgattact agttcgtctg ttgtaagatg
      481 ggtgtaggtc atttgtactc actccttatt aattctttgg tcggaactat tttgagtgta
      541 gcacaaatga tctttttagt tgtctagctt aattttacaa tcgacgcctc aaaaagaata
      601 cccgaaattt aaacagcaaa aaaagatagc gcttaccatt gtgtttaaga taaaacgcta
      661 gctaagttgg ctagcgcttt attaatctaa gcaagctcta aaattgcttc aatacaaaat
      721 caactgctcc tttaggatca cgtgttaaat tgatatattc tgcaccttta gttgcaatta
      781 cttttatccc taatttgtct gcatctttac ggagatcatc caaataggaa ttcacttgag
      841 gtgctagaac gattaaatca aaatttttca tcatatcata atgagaacca taagctcctg
      901 ctttagcaga aagattaaca cctgtctctt ttgcgccctc ttctaatgca ttagccaaca
      961 ttgcactcgt tccggcacca gcacaaagta ctaaaacgcg ctttccttca ccaaccgatg
     1021 cttttgcttc atcaactata actgccgcag ttgcaccaac ttcttggatt tcttcgacca
     1081 attcttcttt tacttcttct gcagcaatgt tttttaactc ttgttctact aattcagaat
     1141 cataggcctt aacaaaaggt aaataaataa tcgcatcgac aacaatcaat actaccgcta
     1201 atacaatcgc caacacacta aacccggttc ctaaaaccaa gccaatcggt gcgggcgtcg
     1261 cccaaggcaa tacggatata aagctattca tccccaagac atcgacaaaa agtttgaata
     1321 tccatacgtt agcgattggt gctaataaaa atggaattag aaagtacgga ttcaaaataa
     1381 taggagtagc aaatagtaac ggttcattta ccgcaaacga aactggaata aatgaagctt
     1441 taccaactgc tttcaattgt tttgatttag cgaacaacat gaacagaaaa ggaacaacta
     1501 gtgtcgctcc agtaccaccc atcgttccaa caaagtttcc tagaccaact gttaatactt
     1561 ttgaggcttg tgcaccgttt ttaaataatt caaagttagt ttctacgttc gcataaataa
     1621 ttgcagcaat cgctggttca acaatcgatg gtccatgaac accaacaaac cagaacattg
     1681 ccatggctcc ccaaatgatt gcaataccga catatccatc agcagcacta aataatggct
     1741 gcaataattg aattacacct gctgcaaatg atgttccaaa aaattgacga actcccgtat
     1801 caatcaaaac ggcaataaat actgcaattg aaaaggggat aatatctcta aacatctgtg
     1861 tgattgttcc gggaacttct tttggcatac ggatcgtcaa atctctttta acacagaatt
     1921 tatacacatt tacagtaaca aacgcagaaa cgaaagctgc taataatccc tttgtcccta
     1981 aataatcagt agctatccca ccatcaattt gatcagctgc catcattaca aaaccacaaa
     2041 ttgacgctaa cataacagaa acttcattaa tctgcttacc tgaatcagat aattgacggt
     2101 tcattgaatt agctaaacat cgagctgttg tagcaacaac caaaagacca actaatccca
     2161 ttgaataacc ataaactttc caaagccacg tcgagaatgc ctctggtaac gtcacgttaa
     2221 atacttctgg aacagccgca atcataataa aaatacttga aaatagaata gctggcatag
     2281 cagttaaaaa gccatcgcga attgccccta aataaatatt acgtgagatt ttttcaaaaa
     2341 acggttttcc cttctcaatt tgcttaataa tcgtattcat ttttcttctc ctatgcttta
     2401 gcaaattttt ctttgtacaa ttcaacaaaa tcattgataa gtt
//
LOCUS       DAEGIQ010000208         2415 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000208 DAEGIQ010000000
VERSION     DAEGIQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.219"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            483..1808
                     /locus_tag="KMT45_002892"
     CDS             483..1808
                     /locus_tag="KMT45_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBE7006296.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            1801..2148
                     /locus_tag="KMT45_002893"
     CDS             1801..2148
                     /locus_tag="KMT45_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006297.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
BASE COUNT          915 a          329 c          459 g          712 t
ORIGIN      
        1 gctaatcgaa gtaaaaccca aagccatatg aacccctgaa tcagcgaaag tttatgatga
       61 tttggattac taatagttta ggaattagat ggtaaatcta aattaaacaa tcgttgattt
      121 ggcaacgaaa tagtgaggga aaatccactc actgaagggt aaactccgtc aagttatgtg
      181 accagacgga aggacgaatg ttgttgaggt tttagaacca tataaaacag catagttctc
      241 aaattgttta aaatgattgt caagttaaaa aaatctggga tcatataaaa tagtatagtt
      301 ctcaaatatg attagaaacc ctattaaaga gcaatattaa gccaacttac ttaaatcgtt
      361 taattaagaa caataatctc ttttagtcag acttttagtc tgactttttt gtatttccaa
      421 aatttattct ttacaaaaac gaacatacgt ttgtataatt gcttgtgaat gggggaataa
      481 aaatgtttga ttacagtaga gaaccggtga atgactattt tttaatcgac atgaagtctt
      541 tctatgctag cgtggaatgt attgaacgaa atttagaccc aatgactaca ccgttaattg
      601 tcatgagtag atcggacaac actggttcag gtctgactct agcttcttca cctactgcaa
      661 agaaaaaata tcatattact aatgttagta gagctactga tttaccttat ccattaccaa
      721 aagaacttca tattgttcca ccacgaatga acttatatat aaaaaaaaat atggaagtta
      781 atcagatttt tagaaagtat gtcgcagatg aagatttatt gatttattcg attgatgaat
      841 caattttaaa agtgacaaaa tccttaaatt tatttactag ttcaggaaca agaagcgaga
      901 gaagaagaga attagctaaa ataatcaaag atgagattca gaaagaaaca ggtctaatag
      961 caacagtcgg aataggagat aacccattat tagctaaact tgcactggac aatgaagcga
     1021 agagtagtaa agaatttata gctgagtgga cttatgagac tgtacctgaa aaaatatgga
     1081 aaataaacga gatgactgac ttttggggca ttggatcacg catgaaaaaa agattgaatc
     1141 atatgggaat tatgagtata gaagatttag caaattggaa tccctacaca attaaaagtc
     1201 agttaggtgt aatcggactc cagttatatt tccataccaa tggaattgat cgtacggata
     1261 ttgctattcc accagaacca accaaagaaa agtcttatgg gaattcacaa gttctaccaa
     1321 aagattatac gaggagagaa gaagtagaac tagtagtcaa ggagatggca gagcaagtag
     1381 ccattcgaat tagacaacac aattgcaaaa ctggttgtgt acgcttagca attggtacgt
     1441 caattttgga agaaaaatca ggtttctcgc accagatgaa aataccaatt acagataata
     1501 cgaaagaatt acaaaattat tgtctttttt tattcaataa gttttatgag gggcaggaag
     1561 tacgacatat aggagtaact tatagcagat tagtatacac agattcccta caattagatt
     1621 tatttagtga tccaagaaaa aagattgctg aagaaaattt ggataggatt attgataaga
     1681 ttagacaaaa atatggattt acagctatta tacatgcttc atctatgtta gaaggtgcaa
     1741 gaagtattac gagaagcact ttaataggag gacacgcagg aggattagga ggtttgcata
     1801 atgattgaat ataacgatag aggacgttta aaatggtcgg gattttattt gtctgatcat
     1861 actgaaagaa tcaatgcaga caatgtacaa cgtgctaatc ataatttggc aaaagaacaa
     1921 atgacgactg aagagattgc gaaaatactg agaactgctg tcataaaaaa tagacccgtc
     1981 tgcattcaaa aagaggaacg aaacgctgaa ggttactatc cagatgatgt agtaggaaaa
     2041 attattggat atgacgagct tggtgtattt gtggaagaag aaaaagttca ctatgatgaa
     2101 attaggaatg tttctttctc ggaattatta aaatggagtc agatatgaac attattaaaa
     2161 aatacgaatt cggttatatt acgtatgaac agctaataga ggaattatgg ggatatggtc
     2221 aacaattaat taatgaagtg gggattgatt gcttttgttt ctatattgaa tccggagcag
     2281 gataccataa gaacagatac tatatatttc catatccacc aaaatagtct cttagatatt
     2341 tttaaatttt ctccaaatac tttcaattat tgtgaaatat ttccctttaa ttaatcaaat
     2401 tttttataaa agtaa
//
LOCUS       DAEGIQ010000209         2402 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000209 DAEGIQ010000000
VERSION     DAEGIQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.220"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            26..238
                     /locus_tag="KMT45_002894"
     CDS             26..238
                     /locus_tag="KMT45_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006298.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(516..2315)
                     /locus_tag="KMT45_002895"
     CDS             complement(516..2315)
                     /locus_tag="KMT45_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBE7006299.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          840 a          439 c          450 g          673 t
ORIGIN      
        1 aaaaataaag aaaagaggga atgaaatggt aaaagcagaa gtaggaagat cttatatatg
       61 caaaccgatc ggagtatctc atgaactaat cggctgtatt gaacgtacat atatcaacac
      121 ggctgtcgtg tatgttgaaa gttatgacca acgagactat caacttatca atgaaagcaa
      181 gtatcgcatg ctcgtaaaat tctgtgatat tagcgcacct actgaattgg tttcttaaac
      241 agtatttttg agaattcgca agaactccct tataatataa ataaagacag aagggagcga
      301 gaagatatgc caaaaagaaa aagttcaggg aaaatgggaa ttattcttgc agtgatctct
      361 gcaattcctg ctattttagc tattgttcgt gcgatgaagg aatcaagaga aagttaagca
      421 cacattatga gcgtcaaaca aaaaacgcat atacctgcgg tggcaggtta tgcgtttttt
      481 gttgtataaa gacttttttc attaacgatt tttgattagc cgactcccaa aacaaaattt
      541 agcagtttat ctgaataagt gatttcttca tctgtttgtt tcacactttc atttagtagt
      601 cccatctcat cggttatgat accatccaca ccataaaaca tcatgcgact catggcatct
      661 gaatcgttga ttgtccatac atatacttct tttccgtctt ggtgggcaga attaatgaag
      721 ttacggttga tcgtactata ttccattgtc agaaaatcag caggcgtcac agggggccca
      781 acaatattga aaggtaagat gtagcccaca taaaagtttg gttctgcttc ttttaaatct
      841 gaaacggttt ggtacgtcaa actttgcaaa atatgatggt tctctaagat aactttgcgg
      901 tatttatgga caaatcgttc gacaaggtca tccgaatctt ttttagaggt cttgatctca
      961 ataagtaatt tttggtttag cttatgtgct ttttccaaat aagaatcaaa agaacaaaca
     1021 ggggcttgca ctccgttttc agtaactgta agatttgtca attctttcaa tgtaagatcc
     1081 tgtggtgttc catttacccc tgttaagtga cgaagatttg catcatgcat cactacgaaa
     1141 tgaccatctt ttgtttcctg aacatccatc tctacataat caggatgata gtctctgctt
     1201 gttttttcaa gtgcttctaa agtattttgc gccccatttt gggtactcac acctctgtga
     1261 gagacagtca ctggttttgt ctgaacagct gaagtcagat aatccatatt gtaaagacag
     1321 acgccaatac cgaaaaaaac tgcaaaaaga gtcaaaccag tgtttttcag cgcagagaaa
     1381 cgcaagttgg gggcttgtgg gataaaccac ttagggatct caggcaagaa gccttcgtca
     1441 tccatgaaat caacaatgat ataaaaaatc cctacggtag acatcacgat attgaataat
     1501 aaaatccctt gtaaaagcgt catagcaaac acagcgctga tcaatgcata gtctgggaaa
     1561 aattgttcaa ccatactttg tgccaaaata acaacgatat atcctaaaga agaaaggagt
     1621 aaaatcgtac cgccaatcac aataaattgg cccgtgatgg ccaaaagacg cgatttggtc
     1681 aatgaccagc tttcccgaat ggctgctcga aaagaacgat ctcgtagaat catttccggt
     1741 aaagcaaaaa tcagtcgaat cccaatatag ccaaggaaaa tataaacgag taaaaaggaa
     1801 ctgacgatga tccaacgatt agcaaaaata aaatccataa tgaaagcggg gatttttatt
     1861 tttgacagta gatcagaatt aaaacttaat ccgctgatcg gtagaatcaa taaaaaataa
     1921 gcaaggaaaa acaaaaaagt aatgggtctc actttcttca gctgaagtat cgtcaagtgt
     1981 aagagttgct tcaaagagat tggttctttc tttttaataa aaaaaacact catcaaaaga
     2041 aaagtaaatt cgaaataaac taacagtaaa atcgtcatca aaactacgat taacgagagt
     2101 agaaccccag gatgttgaga aaaaattgaa ggaatattgt cataagacaa atagtcaatt
     2161 gcccctcggt gtaaaataaa acgggtcgta ctgctcaata agggtaaaca aataaataaa
     2221 ataaagccat gcatcagcaa gacatcacgg aaataagctt gtgttccttt taaaaagtcc
     2281 caagcattac ctaagctacg tttcaaataa ttcatcctat cacctgctct tcacattgta
     2341 gcgcaaatct aatgaaaaag gaaagggtgc agtgtgtttt gcagaccctt tcccagattt
     2401 aa
//
LOCUS       DAEGIQ010000210         2375 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000210 DAEGIQ010000000
VERSION     DAEGIQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.221"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(117..707)
                     /locus_tag="KMT45_002896"
     CDS             complement(117..707)
                     /locus_tag="KMT45_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBE7006300.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1230..1391
                     /locus_tag="KMT45_002897"
     CDS             1230..1391
                     /locus_tag="KMT45_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBE7006301.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1410..1904
                     /locus_tag="KMT45_002898"
     CDS             1410..1904
                     /locus_tag="KMT45_002898"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBE7006302.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1897..2058
                     /locus_tag="KMT45_002899"
     CDS             1897..2058
                     /locus_tag="KMT45_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006303.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          846 a          363 c          396 g          770 t
ORIGIN      
        1 atgaatataa atagagtgta tcttctttga taaatgtcag aaaaagtgtg acaaataacc
       61 atcagtaaaa ttacttgtca tactattgat aaggagtatt taattgaaga gaatgattag
      121 gagaaaggat ctccgtactt tttatagtaa tcatagaatc caagtttgta tagacgaatc
      181 tttttttctc ttatttcttc tgtttcaaaa tggactgctt gaagaatcaa gtctgtgaat
      241 tctataggag ctgttccatt cgcagtaatc aaattacgat ctttcacagc ttgttgttct
      301 ataaaatctt ctttattctg ataattttcc gattctaacc aaggaaattg cgcatttccg
      361 gtgtgtttgt aaccttgcaa taatccgctc tgagctaaat aatcaactgc cccacaaata
      421 gcagctactg gtacgtctgt ttttaacaat ttatcgatta tttgatataa atgatgattt
      481 tttatgtccc atgaattgcc accgatcata ataaaaaggt caactttttc aggtatttgt
      541 tcgattaagt aatcgatttt tgtttttaat cctccaatgg aagtgacctc tgattggata
      601 gaagcagttt tgttttgcca tccctctttt tggtttagtg tgctagtaat ataagcacct
      661 tcccaatcgg catattcctc taatagaaac gtaatcgcag ttttcatttt caagctctcc
      721 tttactatct atgacaagga caacagtttt aacatacaga cattcattaa atttggatag
      781 cgacagcgaa aacaacaatt cttaagtttt agacagagta tacagacagt caacttaatt
      841 aaatacgaac taaataaaaa tgacaggtaa tcatttttgt ttaccagcat tataacattt
      901 tgcatacatg gatgacaaaa gaaccaattc ccacttgctt gagtacaatc ttaaaagaaa
      961 ctggaaggca aaaagtaatt atagaaacga aaaataaatc atatcgggca ctcagacatg
     1021 aaaacaaccc tagaaatata taactagtcc aatgaaaaaa aagcgaatgc cgtagaaaac
     1081 aaggattctg cgtacattca tatctcattt tgcgagcata tctgctatta ctgtgactaa
     1141 atgttaatat ggttaaaatg accgttatat tttttatata aatatcgtat aattagtatg
     1201 aatatagaag aaggaaagaa ggtgcaatct tggaacctaa agacaagtat ttagattacg
     1261 ctagggtcaa taaagataaa tttattgaaa caatcatcaa gggaaaaatc cctgatgatg
     1321 aaaaagatgc ggtattcatg gctggaagcc caggagcagg taaaacagag gttgcactag
     1381 gtttagctta aaattatgac aaccatgttg tgattgatgc ggactatttt agagcacaat
     1441 ttccagaata caatggaaag aactcaagtg tttttcagaa agcttcttct tggttagttg
     1501 aacaatcatt aaaatatgta ctggaacatg gctattcttt catactagag gatacttttg
     1561 ctatccttag tgcagagaaa aatattattc gagcccttaa aaataacttt cgcgttacga
     1621 ttttctatgt ttaccaagat cccaaagttg cctgggattt cactcgaaaa cgtgaactag
     1681 ctgaaggacg tcatgtgcca aaagaaatgt ttatcaatgc gttttttaag tccagagaga
     1741 atattgaaaa agtcaaaaat agacatcctg aagtagtcct tcacataatg attaaggatt
     1801 atcaaaacga catttctgaa gtgcattatg cagcagataa tattcaatta gtactaccaa
     1861 tacaatatac ttcaaaagag ttggaggagg aattacatga ctgagaaaga acaagtacaa
     1921 caaatcgtga agaagtataa taaaagtatc gctgatttat cagaaaacgc ctcagcgaaa
     1981 gaatttaaaa cagtaatgaa ttacgtagca gatgaagcaa atcgtaagca gcgaaaacta
     2041 gttgggttgg ataaataaat tttttaaacc atatccttgt aaagttgagt tttttatttt
     2101 aatgaattaa gactattaaa tagataatta ttgtaactta aaaaacacag ctaaaaatta
     2161 gttgtgtttt ttattaggtt tacttttctg gtataaaagg aactagctac tttttcaaaa
     2221 tttctaaatc attcaatatg tagcgcttac taaattattc gccataaaat tttataattg
     2281 gatcactcat tgttttacct aagtcatatt taagtaataa ccggtgtttt tacataagaa
     2341 gacttcgggt attttttagt ttgattagct ctgat
//
LOCUS       DAEGIQ010000211         2348 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000211 DAEGIQ010000000
VERSION     DAEGIQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.222"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..542
                     /locus_tag="KMT45_002900"
     CDS             <1..542
                     /locus_tag="KMT45_002900"
                     /inference="COORDINATES: protein motif:HMM:NF024997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE7006304.1"
                     /translation="SKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLHT
                     KRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKI
                     RHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHS
                     SIHYLTPQEFEELAKEKMA"
     gene            complement(1015..1587)
                     /locus_tag="KMT45_002901"
     CDS             complement(1015..1587)
                     /locus_tag="KMT45_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7006305.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1603..2208)
                     /locus_tag="KMT45_002902"
     CDS             complement(1603..2208)
                     /locus_tag="KMT45_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBE7006306.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          467 c          358 g          783 t
ORIGIN      
        1 tttcaaaaga gaatctctta aatcaggact tttctactga aacgatctgt gagaaatggg
       61 cagctgacat tacgtacatt cctactaaga aaaatggttg gtgttactta tcttcaatca
      121 tggatttaca cacgaaaaga attattagtt atacattttc aaaacgaatg actgtggatt
      181 gtgtcattca aacattgaat aaagcaaaaa tacattatca cattccagaa ggaatgattc
      241 tacacactga cttgggcagc caatacacag caagagaagt ggaacaatgg cttaaaacca
      301 acaaaataag gcattcttat agtagaaaag gaacacctta tgataatgct ggaatcgaat
      361 ctttccatgc ctcattgaaa aaagaagaag tctacacgac tagctactca gattttgaag
      421 aagcaaatcg agcgctattt agctacattg aaggatttta taaccgaaat cgaatccata
      481 gctcgattca ctatctaacc ccacaggaat ttgaagaatt agcaaaagaa aaaatggctt
      541 aaccgtctct actaaaatgt gtccaagata ttgactcaaa tccagtaata tcacaatagg
      601 aatgctgttt attatattag cagcagtgct gatttttatg atagttattt ggttcttaaa
      661 tgaaagatct aaagacaatc aacaccgtag ggatatggag ataaaatcaa tagagtcaat
      721 agattattta tcgataataa cactaaaaaa gccacgttcc taaacgtgac ttacgctgaa
      781 cgatatgtgt tgtttggtag acgaacaaat cgttcattat aaatatatga tggtatccta
      841 atatagtaag cgtggctagc atacctttaa ttactttacc ttttacagtt accatacaca
      901 ttttttgagc agaaatcaag taaataaaaa aatattatat ttcacaacat gctaatagac
      961 tcatatctag aacgcattaa gtatttacct gtctggttcg ctgagacttc ttttttaact
     1021 ttcttcaatt ttcttcttta ttctaaaaag cgtactgcga ctgaaaccgg ttatagcttc
     1081 aacttcctta tacgatttac ctgaagtaag cagttcatat gcgtgcctct tcttcggtgt
     1141 gattgttgct ttcggacgtc cttctctgaa attaggatta ttctttcttg caaacatttt
     1201 tccttcttgg gtacgttcca caatcatatc acgttccatt tctgctacac tcaacaacgt
     1261 ccgtgtcacc attcgcccca tggacgtatt ttcaatcgtt cctagattca aaaccttgat
     1321 cgttacgtta tcatcgagta aaggttcaat gatctctaat gcttctctcg tgttacgagc
     1381 aaatcgatca agcttcgtaa caactatttc atctccaggc ttcagaccag ccaacagctg
     1441 attgaatttt ggccgatccc gttttgtacc cgtaaatttt tctgaaaaaa tattcttctt
     1501 ctctacgcag ttctcaagca actgttgaat ttgttctttt gtactttgag aagcggttga
     1561 gacacgtgca tatccatatt tcgtcatttg gtccacctac ttttacaaat catctatcgt
     1621 atactcagaa tacccttcat agaaatagct gacatcgatt cctttcatat ataactcgcg
     1681 atcgtcaatt ttatcagtca gagcattgga aattagatag cgtatttcca aatcgtttat
     1741 gggacttcgc tccattgcag aaagatagtc tgctttatcg attaagttcc aatctacaac
     1801 tttttgcaat tcttccttta aaatgagatc cagccaaatt ctagtacttc ggccatttcc
     1861 ttctcgaaat gggtgagcga tattcatctc cacatatttc ttgataatct cttcaaaaga
     1921 agtttgtggc atctgatcaa tatgctgtaa cgaatgttct aaatacataa ctggtgcaaa
     1981 tcgaaaattt ccttttgcaa tattgaccga tcggattttt ccggcaaaat cgtaaatatc
     2041 cgaaaataga aattcatgaa tttcagataa acctttaaat gtaccaactt cgatttcatt
     2101 tatttttcca caatcgtaca attctttcgc tttttgttta ctgagttttt cttcaacctt
     2161 agccaactca acttgattgg ttaatcccaa tttattctct aaaatcatgc gaaatctcct
     2221 taactttatg acataagttt atgaaacact ctcaacccca ttatatcgcg attttgttta
     2281 tgtgtcaaaa actattaagt ttctgacccc cgatagattt ttcaagtgtc tctattttgc
     2341 acagtcta
//
LOCUS       DAEGIQ010000212         2336 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000212 DAEGIQ010000000
VERSION     DAEGIQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.223"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            40..2193
                     /locus_tag="KMT45_002903"
     CDS             40..2193
                     /locus_tag="KMT45_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBE7006307.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          802 a          416 c          523 g          595 t
ORIGIN      
        1 acaatgaagc atgaatgtaa ataggcggaa ggataggaaa tgatgaaaga ttttatgaaa
       61 aaaatattaa atacttcttg gttagaaaaa ataagaaaag gtgcacaaga acctaagaac
      121 aaaacgtttc gccggtccca tgttcctttt cgcttaaatt ttcttttttt cattatattt
      181 acgttatttg ttgcgttgat tgctagattg ggttatttgc agatcgtgaa cggcgaagag
      241 atggaggcgc gagtgaagtc tacttctacg attacggtcc aagaaagtac tcctcgcgga
      301 atgatctacg actccacagg aaaagcttta gttacgaata aagcgaatca ggccatcaca
      361 tatacgagag gaacacaagt tacggctaaa gatttgcttg aaatcgcaac gaagctgaac
      421 tcgctgatcg atgttccggt agatgaaaat ctgacagata gagataaaaa agatttttgg
      481 ctggctgatc ccaaacattt gaaagaagcc acaaaaagac tttcggcaaa agaaaagcaa
      541 ttagggaaca gcgaacaata tagtgcgatg gtcaataaag tcacagctga tgagattaat
      601 ttaaacgagg agcaattaaa aattgctacc atctttaaac ggatgaatag tgcatatgaa
      661 ttaaacactg tctatatcaa aaactctggt gttactgatc aagagttagc tgttgtggca
      721 gaacgcgctt ctgaacttcc aggagtttcg actggcacag actggacacg agaatataat
      781 gcagcaagtt cactgaaaag tattcttggc agtgtgacga ctgaaaagca aggacttcca
      841 gcagatgaag cagagaaata cctagcaaaa ggttattctc ggaatgaccg tgtaggacaa
      901 agttatttgg aaaaacaata tgaagatgtc ctgcaaggaa ccaaaaccca atatgaagtt
      961 tcccttgata atgaaggaaa tgtttcgaat caaaaagaaa tcttctctgg ggaaaaaggt
     1021 tctaatttaa tgttatctat gaatgcagaa ttccaatcaa aagtagaaga aattttgaaa
     1081 aggaattatc aaacattgat caacaacggc aaagcacagt attcgcctgg tgcttatgca
     1141 gtggctatga atcctcagac aggagaagtg ctagcaatga ctggtttcag tcatgaacaa
     1201 ggttccaaag aaatcaccga gaatgcttta ggcacgatta ctagcgcctt tgatccaggg
     1261 tctgtagtca aggctggtac tttgacagca ggctgggcaa gtaacgcgat cagcggaaat
     1321 caagttttga ttgatgagcc gattcgtctg caaggtgctt cagaaaaaag ctctgtcttt
     1381 aatcgaagcg gacaagttgc tcttgatgca gtcaaagcat cggaattgtc ttctaatacg
     1441 tatatgatca aagtggcgtt aaagatgtta ggactagact atacacctgg aatgggacta
     1501 cctagtttag atgaagaagc taaagcttat cagcaattga gagactcttt caaagagttt
     1561 ggtcttggta caacgactgg gatcgatttg ccgaatgaat ctcctgggat ctcacgttct
     1621 gttgattata tgaagaagtt caatgcggat aatggcaaag aatggtatgc tccaggtaac
     1681 tttaccgatt tggcatttgg acagtttgac acatatacac caatccaatt agctcaatat
     1741 gcttcaacag tcgctaatgg cgggaaacga gtacagccgc acctagtcaa agcgatctat
     1801 ggcaatgacg aaaatggaaa cttgggtgaa gtgaagaaag aaattggcac aacagtagaa
     1861 aatacggtca atatttctgc agaaaatatg gcgatcctcc gtgaaggatt ccaccaagtt
     1921 gtccatggta ccgatcctta cacaacagcc aaaccattag ccagtgcgaa aatggacctt
     1981 tccgcaaaaa cagggactgc agaaacggtt gcggaaggtc atccagatat tgcgacagtc
     2041 aatagtaata tcgtggcata cggaccaaca gacaatccgg aaattgccat cagtgttgtc
     2101 ttgccgaact tattagacga acaagaccac ataaacttga cgattgctaa agaaataatg
     2161 gacgcgtatt atgatatgtt catggcaaaa taaaaaagag gtcgtgaaaa agccgcttag
     2221 tttcgagtat gaagaaaaat tttgtgaaaa tagcttctca catttttgct taaatttgac
     2281 ttcataccga gaaactggct tttgaacacc gtttactcgg caaaaaaggt tgtgac
//
LOCUS       DAEGIQ010000213         2321 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000213 DAEGIQ010000000
VERSION     DAEGIQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.224"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..1167
                     /locus_tag="KMT45_002904"
     CDS             82..1167
                     /locus_tag="KMT45_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBE7006308.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            2003..>2321
                     /locus_tag="KMT45_002905"
     CDS             2003..>2321
                     /locus_tag="KMT45_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBE7006309.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMG"
BASE COUNT          892 a          343 c          394 g          692 t
ORIGIN      
        1 tattcaccct taatacatgg tatagttcac ttgttgaaaa tcctgaatat atacaagttt
       61 ctaagaagaa ggagaaattt aatgtctaca gaaaatcaat ctcaaaaaat gataattaaa
      121 aattggggat ataaagcaac gaatgtttat attgaaaaat attataaatt tcctagtttt
      181 ttaattcaga attctaaata ctctaaaatg aaagacagct caaaaatagc ctatatgctt
      241 tttaaaaatg aatttcgtag agccttacat gaaaaatgga tcgacgatga tggtctatta
      301 tatttggaat ttacacaagc agacctaatg gacaaattaa attgctacca aggcaaagta
      361 aaaaccattt taaacgagtt agaaacatat aatttgattt cagtcgtaaa aggagaattt
      421 aacccaaaaa cgaaaaaaaa tgaaaaaaat aaatactatt tacttcaacc tgaaataagt
      481 gccgatgatc tttttattca aaattctttc gatgacgatt taagcgaaac tattgataaa
      541 tcaacctctt ctcggaatgc ggaaatcgca cgtcgagagg aaactgctaa aacccttgat
      601 aacactggaa atgcggaaat cgcacgtcga gaaaaaacag ctgaaaccct tgataacact
      661 ggaaatgcgg aaatcgcaca agattattat attaataaat ctttagacac taaaagacac
      721 ttaaaagaca ctgaaacaga ccaattacaa aatcaagttt tgctagataa cttcgttgaa
      781 atcatgaaag aagacagcat aaacacattt gtaccagaaa aggtattaag cttgataaaa
      841 acattttcta acacttacga agaagcccaa caaaccgtca ccacaataca caaagcaaaa
      901 cataaagccg aggaacttga gaagacggta gttgtgtatg aagaacttga atcttacgga
      961 attgatgcgg ataaaggatt atatatgacg ttgctgaaag cttatcaaaa acaaaagact
     1021 gagaaagtaa ataatttgca gaatttgatt tttgtgtatg taaaaaactg gtttattgaa
     1081 aaagctattg cggcaaagct tgcaaacgaa caaagagaaa catttaacga attaccaaca
     1141 gttagtacgg aaaattggct agagtagttg ataaaaagcg aacactataa aaatgatcaa
     1201 ttttaaatta gaactttttt acaattttaa ttttattaca aatgttacaa ataaatagtt
     1261 attattccgg agtttttaga ttaaaagttt atgaaaacaa tcacaaacgt aggttttaat
     1321 gtcataatcg ttagatattt tagttatttt tcattcaaaa aaacagggaa ctagtgtttg
     1381 catatgtatt tttttgagtt ataataattc atgaagagag gtaattcact tatttaccca
     1441 agctgaaaag cctgatatta cttcttttca ggcagtgctg ctaatattca attacacttt
     1501 tttgtataaa tttagttgtt ttaaatttaa ttgtctgcta tgcttgtgat gaattgattt
     1561 tcgttctgca cttttggacg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcc
     1621 ctgtgagact cggaaggcta ccaaccttcg tcgacgtcca caggtggcat taagatcact
     1681 cccttaatgc gtttaccttt cagtgttacg gtattacccg taacggattt agtataacag
     1741 atattcaaaa gtgataccac tttttttggc tgtgttaagg ttaaaaatac gtgatattac
     1801 gatgaatcta tgcctaagca taatgttata aaaatgtccg tgtggctctt tgccgtatga
     1861 cgaacgctga aattagaggg aacgcatgtt tggatgcgtt ccctcttttt tgtttgtcat
     1921 gaacgaaaat caattctcta tttttgaaaa ataacaagaa aaacttgtta aaaatattta
     1981 aagtaagtgg gtggattatt ttatgtcata tacaggaatt ttgagtctcg aagatatttg
     2041 tcactacggt aaacggtgta ccgctacaga aaagatcacg aagaaactat caactggtca
     2101 aagtaaaacc gttgtgcagt gtaagaaata tataatccag aaagacaaag tctcagaaga
     2161 aatgatttat tacattggga aacaaaaaca aataatttta aaagatccta tccctttaaa
     2221 agagctatat ccaacaatca aacatgttta tgaccaaaac ggtgtactga ttggtcgaag
     2281 aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg c
//
LOCUS       DAEGIQ010000214         2310 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000214 DAEGIQ010000000
VERSION     DAEGIQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.225"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(81..362)
                     /locus_tag="KMT45_002906"
                     /pseudo
     CDS             complement(81..362)
                     /locus_tag="KMT45_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            824..2206
                     /locus_tag="KMT45_002907"
                     /pseudo
     CDS             824..2206
                     /locus_tag="KMT45_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          844 a          394 c          384 g          688 t
ORIGIN      
        1 gattcattac tctgtcctct ctgtcttttt tctcaatttt acactaaaat agattttttg
       61 gaaaactttg caacagaacc gaaatgagta tagtatgaat tagtaatttg gttttcatct
      121 aagtttagct ttgatttact aaaattgata tctcgataca tgttcaatac atctaataca
      181 aatcgagaat cttctttcgg aaatggctca gaaagatcac tccaaatttc atcatcataa
      241 tgatactcgt aaccttctct caatatttcg atttttcttt cataataaga tctgttttgg
      301 tcctcaaatt tagccaataa ctcaaattga tttactagtg acaatctctg aaaactgtcc
      361 attaaatgtt tcttcctatc ttgttcatta acaaaaaatt ataagccctt acaacgaata
      421 ttatatatta aatttaattg taggtgaaca cgaattttat aaatatttat ggtgttaatg
      481 acaaatgata tccgactcct cttcatacgt tctggactat ttgctaatag attaaaattt
      541 cagcatctat ttctgaccat aaataaatga gctgccgaaa agctgccaaa tcagacttga
      601 aaataaagga tcaaaaactt ttaagtttct gaccccgtaa ctcacacttc aaaatcttac
      661 ttaaaaagga gtctgaaaga aaaaaagagt attcaaaatt tttttctaac tagctacatc
      721 acatctatgc attgacactc aaagaaccag aataagtcat cagaaagcaa gggggtgcgg
      781 acataatcct ggacgaaaaa ataaacttag gaggaatgtc cgtgtgtttt cccacaaaga
      841 gcgtgtaagg gcaattcagt tatttttaaa atacgattgc tcttatgctg caaccattcg
      901 agaattaggg tatccgtcga ttggtgcact acggaaatgg tataaagagt atttggaatc
      961 cggagagctc catcaggagc accgaaaaaa atcaaagtat tctgaagaac aaaaacgaat
     1021 agcggtgaat cattattttg aatatggtca atgttatgca cgaaccattc gcatgcttgg
     1081 ttatcctaat agagagtcct tgcggcagcg gtgtgaagaa cttgcaccag gagctagaaa
     1141 actccgaaaa agtgcggtaa agttaacaca agatcaaaaa gaagctgtgt tgaaaaaatt
     1201 ctataaaccg caaactaata ggaaaaattt agccgaatct ggaggaatta gtagagttac
     1261 tctatatcaa tggaaaaaca cggttcttgg aaaggatttt ccattgtgta tgactttaaa
     1321 aaatcaggaa aaacaaaaag agctactttt aaaggaagta gaagacttac aaaaacaagt
     1381 acaccaatta cagctcgaaa aagcattact cgaaggagcg gctgaactgc taaaaaaaga
     1441 aaaaggcgtc aatcttcttt gtctatcaaa tcaagaaaaa acgatattga ttgatgcctt
     1501 acgaaatcag ttcaccttga aagagcttct tcaacagctc cagtttccta aaagcagcta
     1561 tttttatcaa aaacaagcac ttgagaaacc agataagtat tacaaagaac gtcaaattaa
     1621 ttatcacgat tttcaatcat aacttctgtg cgtatggtta ccgtagaatt caccaagctt
     1681 taaaaaatat gggtaaaaag ctttctgaaa aagttgtacg tagattgatg actgaggaaa
     1741 atctatttgt taaattttct agacgaaaaa agtattcttc gtatgctggg gaaatttctc
     1801 ctgcacaccc caatttatta aatcggaatt ttaaagcaaa gaagccaaat gaaaaatggt
     1861 taacagatat tactgaattt agaattctcg cagggaaaat ctacttatca ccacttgtcg
     1921 attgttacga tggtgctatt gttagttgga ctattggaac aaagccaact tgcttcactc
     1981 tatgtctaaa aagagttatt caccagataa ttcagcgtgc gaaggtttct ttggacgatt
     2041 aaaaaacgag atgttttatc aacgtgactg gaagaataca acaatcaatc agtttataaa
     2101 caaattggat gattatattc attggtataa taatcaacga attaagttat ctttaggaag
     2161 gctaagccct cttcaatatc ggaagaagca aagatatatt tgttaaaaag gtccaaaaaa
     2221 aatatccgca cccccttttt tttgtaaaaa actattgcac aatacaagta gcctacggct
     2281 atcgaaattt ccataacttt cgagcaagaa
//
LOCUS       DAEGIQ010000215         2270 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000215 DAEGIQ010000000
VERSION     DAEGIQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.226"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            191..445
                     /locus_tag="KMT45_002908"
     CDS             191..445
                     /locus_tag="KMT45_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE7006310.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="KMT45_002909"
     CDS             446..763
                     /locus_tag="KMT45_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE7006311.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="KMT45_002910"
     CDS             1322..2257
                     /locus_tag="KMT45_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006312.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          429 g          711 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag
//
LOCUS       DAEGIQ010000216         2250 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000216 DAEGIQ010000000
VERSION     DAEGIQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.227"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            84..1697
                     /locus_tag="KMT45_002911"
     CDS             84..1697
                     /locus_tag="KMT45_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE7006313.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNNYTSARILAGLGIKAILIEE"
BASE COUNT          707 a          399 c          444 g          700 t
ORIGIN      
        1 aataaacaat aattttccgg cacaattatt tgctgttttt taccataaca tagtacgatc
       61 aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat tggcggtgta gctggcggtc
      121 cttcatttgc cactcgtttc cgtcgattga atgaagcgca cgaaatcatt atctatgaac
      181 gcggagaaaa tatttcttat gcaagttgtg ctttgcctta ttatttagga ggtgtgatca
      241 ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa aacaacatcg
      301 atgtctttac taaacacgaa gtaacagcaa tcgatccttc tactaagcga ttaacagtta
      361 aagacctatc cacaaatgaa gaaataaaaa cagattacga taagttgatc atctcttctg
      421 gtgctagacc agattatccg gatattcccg gagtttttga agcagaaaac ggctttgtcc
      481 tccgtagtgt gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa aatccacaac
      541 atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt agctgagaat ttcaagcatc
      601 gcggcttaga cgtgacttta atcgatcaat tgccacaagt cgctttccct tatgatccag
      661 aaattgctaa tttagtttat gacaaattgc tgaaagaagg attagccgtt catttagaaa
      721 caagagttac tgagatccgt gataaaggtc gagaaatagt attatcagat ggctccgtcc
      781 tttctgctga tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa gtggtgaaag
      841 caaccggcat acaattatca gatacaggtc agatcattgt tgatgaccag ttacaaacca
      901 atctgccgga catctatgcg attggcgata ttatcgaaac gactagtgta gtgactggtc
      961 agccgatcca aagtatgctt tccagtgcgg ccaatcgtca aggacatatg ctggcagata
     1021 ttttaaatgg tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc gcaaaaatct
     1081 ttgatcatac agcaagttat gcgggaatga cagaacatga attaaaagca tcaggcataa
     1141 caaattataa aactgttttt atcactccct ttgaccatgc ctatttctat ccagaagcta
     1201 caagattaaa tctaaagctg atttttgatg cagatagcgg tcgtatttta ggtggacaag
     1261 catttggaga aaaaggtgtc gataaacgga tgggagaact ttctgtagca atcaccggaa
     1321 acttgacagt ctttgatttg cctgatttgg agttgcctta ctctccaccg tattctacta
     1381 cccgtgaccc gttgaatata gctggttatg tcgcaatcaa tcaaatgacg aatatcgtag
     1441 aaacgatcaa agcaagtgat atacccgaaa acgatttgaa agaagcgttc tttttagata
     1501 tacgtgaacc taataaagca ccttctggca gtatctcggc aacaaaaaat attccaatga
     1561 atgagttacg agatcgaatc aatgaaatcc caaaagataa aaaagtttac attactttca
     1621 gaagaggatt gaataattat acttctgccc gaatcttggc aggtttgggt atcaaagcga
     1681 ttttgattga agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg agttattttt
     1741 acttgaggag taaaaatgaa aaagtatata gggttgttgg ttggtatgta tatagaatag
     1801 aagataaatg tgaagaaatt gtgcattatt tcttcacatt tattaggtta tagtttaaga
     1861 aagtttaaac attgcgcaat ttaattattt tagctgtttc ttttcataaa aaaacatgtt
     1921 gtgactctga gacaaatcat agtccatagt gatgtctcta gagtaactac gcctaatatt
     1981 acgaggcatc atattaatct atcagcacta gtacctaaac tagcatccct ttcgaatatt
     2041 ttttcttata taataaagtt tgctttcact cctctaatct caccaaatcg actggcttgc
     2101 gttgtgtgat actaggaact gattggttta gaacgacttt tctattgtaa tcatattttt
     2161 cgccatcctc gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg tattaaataa
     2221 tttaattttt ttgttcctat ttatttaaca
//
LOCUS       DAEGIQ010000217         2192 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000217 DAEGIQ010000000
VERSION     DAEGIQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.229"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            18..683
                     /locus_tag="KMT45_002912"
     CDS             18..683
                     /locus_tag="KMT45_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE7006314.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(967..2052)
                     /gene="xerS"
                     /locus_tag="KMT45_002913"
     CDS             complement(967..2052)
                     /gene="xerS"
                     /locus_tag="KMT45_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBE7006315.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          707 a          329 c          372 g          784 t
ORIGIN      
        1 tttcttattt ctttaaaatg gagttaagag aaatacgtcc aattgacatt caaaagtggc
       61 aaaataattt attgagcaaa tataaaaatt cttatgtaag aaacatgtat gggatttttc
      121 agatgtcatt agatcgagca gttgttttgg gactacttga aaaaaatcct gcaaaagtta
      181 ttggaaatgt gaagaaaagg aaaactacag ttgatttttg gacaaaagaa gaatttacaa
      241 aagttatttc aactttttat attgatgatt tgtaccaaca ttttggtttt atttgtatgt
      301 ggttactttt tatgaccgtt atgcgtttgg gagaagccac tgcattaact tggaaagata
      361 ttgattttga aaataaaaac ttgcgtgtga ataaaacact ctattataag agagccaaaa
      421 cctatgagtt tgtagaaccc gaaacggctt ctagtattcg tacaatcgaa ctagacgaca
      481 atacaattac ctttttaaaa gcttggaagg agcgtcaagt ttcaatagga ggcatgtcgt
      541 ttgtactttc ttataatgga acacctactc agaaatatac aatccgtcat attatgagaa
      601 gacatgcaac gatcgttggt gttcatataa tccgcataca cggattaagg catagtcatg
      661 cttctttgct tatatcacaa taaaaagatg cacttcgaat taaagaaagg ttaaaacatg
      721 ctgatgtaca aacgacttta ggaacttatg gtcatttgta tctaaattca ttgagagaga
      781 taacaaatga tcccacagat tatatccaaa ctaatttttc taaaatcaaa ccgacaccta
      841 tttacgataa taaaaaataa atgataaaca aaagtaggat cacaccaaaa gaaacacact
      901 agaaaaacta taatacaggc ttttctagcg tgttgtttct tatttccgcc tatttataca
      961 ttatactcat aaagcattta atgcatcgat cgtagccgca ttgtctatat gggtatataa
     1021 agttgttgta ttggtacttg aatgtcccaa ttgttgggca gttaatacca atgaatttgt
     1081 ctgttggtag agtctagtag ctaatgtatg acgcagtttg tgagggctga cttgaattct
     1141 aaagcctttt gaatattttg ctaccatccg ttcgattgca ataccggata tacgcttggc
     1201 ttctccttgg taaattgcta gaaataaagc tttttcgttt tctgttactt tatatcgttc
     1261 tttccgaata gctagataat tagctaaata ttccattgcg aatgctgcta tattgacagc
     1321 atctctttta tttccttttc tagttactac cacattattc ttatctaaag ataggtcatc
     1381 taaattaata tttataacct cggagagtct caatccagat cctaaaatca gtgcattgat
     1441 cgccaggtct ctttctacat ctcgttgata atagatcaaa gctcgtttac tcaaagtatt
     1501 tttatattca ttggcaatat aatctagata attttgtgta tccttatcta gaaataattt
     1561 tttctcaatg gcattggcac gtgcagctaa cgtagtttta tctgtgatgg ttgctacttg
     1621 atccatagga ttattgtcta agtatggttt tcctagagga ttattggaac ttttcgaaag
     1681 atatttaaat agaaccttta atgccgtgat atttctattg atagtagaag tagatagacc
     1741 taagttatta ttatctttga gcttagtttt tgttgtctct ttttttacat tttcttttgt
     1801 gcgacgtttc aaatgagatt tgtacaagtt taggtcatag gcagttaact gtcttatcga
     1861 ggtgatagaa gtatcaggaa cgatagactc actcagaagc cattcataaa agagcttata
     1921 attttttaga tattctaaag tggttcgagg tgattgatct ttatcctcac gatcttgaac
     1981 aaaatctttt atataatcgg gcatatgaag tatgattgta tctatttttt gatatatttc
     2041 tgtatttttc atgtcattca tcctttcaat aagtagggga gaagagtatc tctataattg
     2101 gactatacca attatagaat gttttgtcaa ttggtatgct ctaaaattta ttaatttgtt
     2161 tcaaaaaaag catataaaca ggtatacgtt ta
//
LOCUS       DAEGIQ010000218         2143 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000218 DAEGIQ010000000
VERSION     DAEGIQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.230"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1289
                     /locus_tag="KMT45_002914"
     CDS             <1..1289
                     /locus_tag="KMT45_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006316.1"
                     /translation="RTVDIYYRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVS
                     ISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYF
                     WYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLF
                     ILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMII
                     TLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYT
                     TSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVT
                     VPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISIL
                     TYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1276..>2143
                     /locus_tag="KMT45_002915"
     CDS             1276..>2143
                     /locus_tag="KMT45_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006317.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLL"
BASE COUNT          717 a          328 c          373 g          725 t
ORIGIN      
        1 atcgaacagt tgatatctat tatcgatccg caattgcaga agcaatcgct cttacgatta
       61 tcccagctgt tttatattat gcttatggat tgatattcaa tagagaaaaa aaagtcatcg
      121 ctttatctgt cagtataagt ctacttgtgt atactcatgt cttaactaca ctattgtgta
      181 gtgcattgat tgcttgtatg attatagttc gtttgttttt aagcaagcaa ataaaaaacg
      241 atataaaatt tgtggtcgtg aatttagtaa aggctgtttt tttgacttgt gcattaacta
      301 gctatttttg gtatcctatg ttgcagcaga tgaattatca agaaatacac agtcccttca
      361 aaacagattt gcagctagaa gcactttcta tttcagatag tatcattcaa gcgttaaata
      421 acgatattac aacatacact atggggttat taggattggt tgcgttgatt tttcctttat
      481 tcatactgaa agaaatggag aaaaaagaaa aaatcatcta tgttggcgta gtttctacat
      541 gggtatgcgt aactaattta tttccatggt ttttgtttca aaaaacaccg ataagcttac
      601 tccagtttcc ttggcgtatc ttaggtatcc aaatcttttt ttcttcaatg atcataacgc
      661 ttgtttttat gaaaaagact atgaataaaa aatattcatg gttaatcatt ggcgctactg
      721 tggtgtttct actcgtcacg tctatagcag caaaagaaaa ttacgcaaaa gttatccaat
      781 caaaacacgg tcatattgtt atcaatgaag aaacactccc acgttataca acatcagata
      841 tgggaggatt atacgattac gccccaacag aagcgataaa agaaaggcct cacttagaaa
      901 aacatgaagt aaaagtagga gaatcgtggg aaaaagggaa gtataaagct gcagataata
      961 acattactta cacagtggca agcaacaaaa agcaaaaagt gacagtacca gtatatgctt
     1021 atttaggtac acaggtaaaa gtaaacggta aagtcgtaaa tgactcttat aaaaaacatg
     1081 gcttagtgaa tgtggatgta aatgctggag aaaataagat agaaattaca acaaaatata
     1141 ctccaactgc tctcatggct ttatggatca gtattctgac ttattgttta gtggtgtatt
     1201 caagttttaa acgatttgca cctaataagt tgaaagattc tcgatatgtg actaaaaagg
     1261 agataaacaa gaagaatgaa aataaataaa aaagtagtaa tattgagtgg attaattata
     1321 gttagtagta tatattcttt catctttttc aaaaatacac agagtattta taccactgga
     1381 gatctttctt ttcacttatc tcgaatcaag gggttatcga ctatttttac cagtccgatt
     1441 aattatgaga cattcaatta tactggatat ggtgtaaatt atttttatcc ctttctgact
     1501 ttttttccag ctgtaatgtt atattggatg acaaaaaatt tgattgtaag ctatattatt
     1561 tacgtttggt tattgaactt gtgtacaaca ctcgtagctt atcattatgg cgagcggttt
     1621 ttaaaacaaa aaaaagcggc atttttattt agttgtctct atattttctc tgcttatcgg
     1681 acagtagaca tctactatcg ttccgcaatt gccgaagcta tcgcaataac acttgttatc
     1741 cctgtattat tttatgctta tcaaatcatc tctggaaaag aagaaaagta tccgtctgta
     1801 aagctagcat tgagtatgag tttgctagtg tacagccatg tactatcaac attaatgagt
     1861 acagcattaa ttatcatttt tatttttatt cgtttggttt ctaaaggttt caagaatgca
     1921 gattttattg ctatatttaa aaaattattt agtgcggcag gaatgacatt ggttctgacg
     1981 agtgcttttt ggtatccgat gtttgaacaa atgctctacc aaaaaatcaa caaacctagc
     2041 gtgacaaatt tatatgctca tgcatctaat gtgttcgata gtctgactga agctatgaac
     2101 aacgacttaa ctacttactc aatgggacta gttggtctgt tat
//
LOCUS       DAEGIQ010000219         2079 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000219 DAEGIQ010000000
VERSION     DAEGIQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.232"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(461..534)
                     /locus_tag="KMT45_002916"
     tRNA            complement(461..534)
                     /locus_tag="KMT45_002916"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(498..500),aa:Ile,seq:cat)
     gene            complement(723..1742)
                     /locus_tag="KMT45_002917"
     CDS             complement(723..1742)
                     /locus_tag="KMT45_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBE7006318.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1753..1959)
                     /locus_tag="KMT45_002918"
     CDS             complement(1753..1959)
                     /locus_tag="KMT45_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBE7006319.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          623 a          411 c          344 g          701 t
ORIGIN      
        1 aaaggagata ttttttaagt tagtattaag attgtgtaat atgatgatat ctatatttta
       61 tagtatcagt gctataaaat caaaaataag tcactaatta actaccactc caattgtaag
      121 ccttttttct cacttttttt caaaaatatg gtatgccttt taatagcttc aaatataaaa
      181 gagtttaaag cgtaacacac ttatggggaa gtggcttggg gtgcgcttta aactcttctt
      241 tattatcatc tcacaattta acccaaatgt ctttctattt aaaaatcaaa gtaaaacttt
      301 tcaaatatac agaaatataa ctatgtgaaa catcctttca ttaatccata aaaggataca
      361 taaaaaagcc actcatttga gtggcaatga agaaaagctt tagcttgtat aatactcttc
      421 aaaaaattct aacacagaac gattcaaatg gctacgttaa tgtaccctgt aggactcgaa
      481 cctacgaccg gacggttatg agccgtctgc tctgaccaac tgagctaagg gtactggttg
      541 ttgccacata aagccataaa caatcaacca gtagaatgtg tggcaacaaa cctgttatcg
      601 catagcttgg agtgtgacta ttatgggtga tagtgaagat atgcgataac atcactattt
      661 tatcgaatga tttttatagt tgtcaatata gttatgtaat gctcctcaac gaggagctat
      721 ttttatcgtt taggaatatt taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct
      781 ttagtgtttc cctctggatc attcgttgcc cgcatcatga catagacttt cttattaggg
      841 aagttacgca tgttaaaaga tacatgatag ccaacatttc cagaagtatt ataagcttga
      901 tttacatctg atctataaat tccatcagct cttactcgag ctaattcttt cccagtattg
      961 taatccataa tgaagatata ctcgtattta tagttagcaa tgtgccatcc agctacatgc
     1021 aaatttgcat tttcgatttc tccaaactga tcaatgtggg cgtaattcgt tccatctgtc
     1081 agtgtaggat tagctgcacc agctcgtgtt ggatcaatga caggcttgtt ttcagaagtt
     1141 gttggatttt catcggtaaa tccatgagct aaatcatatg ctaatttttc tttacttacg
     1201 cccatttcag aaagataacc gtaaggatct gtatgatcgc cccagatgtt ttgtgttacc
     1261 cataaatgcg atttgattcc cggttggtta taaggagtgt ccaacgttaa tggaatacca
     1321 tatttcattg ctgaatctct agccaattca acgtatgcct tgtagttttt ctcaaacgtt
     1381 gctttatcat gtgtgtgttg taactcaatc tgcacaggac tgttggcatt agcatacgaa
     1441 ccagcaccgt actgtacata accaggttga ccgacttgat aaacaattcc gccgtctccc
     1501 acaatataag cagtataagc gctagtccat gaacgttgca tatactgcgc ttcattgcgt
     1561 cctgttgctg tttcattagc cgtttcatgc agtaaaatat actgattatt tgctacttgt
     1621 gagctacctt catttgcgcc caaattaaat tcattgttga tagtataggc aaatccgtta
     1681 attggcaata aaaaaagagc cgttaacagg ctcatcgcag taatagtaat tttctttttc
     1741 atatctatcc tcctatttat ctgatgaaat tttcttatac gttcgatttg atacacctaa
     1801 aatactccct agaaatgctc caaaacctgt aataatcatc accgttatat ctgtgtaccg
     1861 ccagttaaca ccttttccga caagaccgac aaaagtcgct aaagcgggga taactactat
     1921 agcgatccat ttcaaaatat caaaggttct attgttcata ctatccacct cccttcattg
     1981 gttgggctac tttctttttc aatcgcttgt tttcttctat caatatttct ctttctgaaa
     2041 gcaaggcatc aatttgcccg tccagttgtt caattttat
//
LOCUS       DAEGIQ010000220         2056 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000220 DAEGIQ010000000
VERSION     DAEGIQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.233"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            493..714
                     /locus_tag="KMT45_002919"
     CDS             493..714
                     /locus_tag="KMT45_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006320.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="KMT45_002920"
     CDS             715..972
                     /locus_tag="KMT45_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006321.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="KMT45_002921"
     CDS             complement(1108..1710)
                     /locus_tag="KMT45_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBE7006322.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="KMT45_002922"
     CDS             complement(1777..1932)
                     /locus_tag="KMT45_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE7006323.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAEGIQ010000221         2038 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000221 DAEGIQ010000000
VERSION     DAEGIQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.234"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..426)
                     /locus_tag="KMT45_002923"
     CDS             complement(<1..426)
                     /locus_tag="KMT45_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBE7006324.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="KMT45_002924"
     CDS             complement(535..1566)
                     /locus_tag="KMT45_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE7006325.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          628 a          331 c          351 g          728 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgctta
//
LOCUS       DAEGIQ010000222         2038 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000222 DAEGIQ010000000
VERSION     DAEGIQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.235"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..194)
                     /locus_tag="KMT45_002925"
     CDS             complement(<1..194)
                     /locus_tag="KMT45_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBE7006326.1"
                     /translation="MKNIARNFFYQSLFQITKIIIPIITIPIVSNALGPSGIGIYNYT
                     FSIAQYFVLVAGLGVAIYGN"
     gene            complement(198..1364)
                     /locus_tag="KMT45_002926"
     CDS             complement(198..1364)
                     /locus_tag="KMT45_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBE7006327.1"
                     /translation="MKVSIFGLGYVGLANALLLSQKEEVKAYDIVETKIDILQKRQSP
                     LEDKEVHEFLERDDLNVEFTKDFEDAVLFGDYLIVATPTDYDEKKNYFNTSTVEEVIE
                     KALLIRPDATFIIKSTVPVGYTLELKAKFQTENIIFSPEFLREGKALYDNLHPSRIVI
                     GEDSDRGQEVADMFKENALDEDIPVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     ESRGLDSRQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEDVPSNIIGAI
                     VEANTTRKDFIAEQIIAKNPKVVGIYRLTMKANSDNFRYSSIQGVMKRIKAKGIEVVV
                     YEPTLDSDDFFNSKVIKDFEEFKAISDVIVSNRVEPLLKDVEDKVYTRDIFRRD"
     gene            complement(1456..>2038)
                     /locus_tag="KMT45_002927"
     CDS             complement(1456..>2038)
                     /locus_tag="KMT45_002927"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBE7006328.1"
                     /translation="ANFQSFHGYDRLIKGIAQYYSEGGDWAIRLNLIGNGNTTVLNSY
                     KSMISNSNLEGIIKVLPGMDSQELDTVFDTTDLAVDTLARFRSGVYYNSTLKGKEYLA
                     RGIPIISGVETELDNFENYEYYFRVPADESSINISDILDFYTKVILQSNKTDKEVIQF
                     IREFGKKNFNFDKMLEPVINKIKSKEKQTNLKE"
BASE COUNT          657 a          400 c          263 g          718 t
ORIGIN      
        1 ctatttccat aaattgcaac acctaatcct gcaactaaaa caaaatattg agcaattgaa
       61 aaagtataat tataaatacc aattccactt ggccctaacg cattcgatac tattggaata
      121 gtaattatag ggataatgat tttagtaatt tgaaacaagc tctgataaaa aaaatttcta
      181 gctatatttt tcatggttta atcccgtcta aaaatatctc ttgtataaac cttatcctct
      241 acatctttaa gcaaaggttc aacacgattt gatacaatca catctgaaat agctttaaat
      301 tcttcaaagt ctttaatcac tttagaatta aagaaatcat ccgaatctaa tgttggttca
      361 taaacaacta cttcgatccc ttttgctttg atccgtttca taacaccttg aattgatgaa
      421 taacggaaat tatcagaatt agctttcatc gttaaacgat agattccgac cactttagga
      481 tttttagcaa tgatttgttc tgctatgaaa tctttacgag tcgtgtttgc ttccacaatg
      541 gctccgataa tgttgcttgg tacatcttca taatttgctc gaagctgttt tgtatcttta
      601 ggcaagcaat aaccaccata accaaatgaa ggattattat agtgtgaacc aatccgtgga
      661 tctaaaccta ctccttcaat gatttgtcga ctatctaaac cacgggattc tgcataggta
      721 tctaattcat taaaataagc tacacgtaga gccaaatacg tatttgaaaa cagtttaatg
      781 gcctctgctt ctgttgaatg tgtcaacaaa acaggaatat cctcatccag agcattttct
      841 ttgaacatat ctgcaacttc ttgtccacga tcagaatcct ctccaataac gattcgtgaa
      901 gggtgtaaat tgtcgtacaa ggctttacct tcccgtaaga attctggaga aaagataata
      961 ttttcagttt ggaattttgc tttcaattcc aatgtatatc ctactggtac agtagatttt
     1021 atgatgaatg ttgcatccgg tcgaatcaat aaagcttttt caatcacctc ttctactgtt
     1081 gatgtattaa aataattttt cttttcatca taatccgttg gtgttgcaac gattaaataa
     1141 tctccaaata aaacagcatc ctcaaaatct ttggtgaatt caacatttaa atcatctcgt
     1201 tcaagaaatt catgaacttc cttatcttct aaaggcgatt gtcttttttg cagtatatcg
     1261 atttttgttt caacgatatc gtatgccttc acttcttctt tttgactcaa caaaagtgca
     1321 tttgctaaac caacgtaacc tagaccgaaa attgatactt tcacattcat tccatccatt
     1381 tcttatcgca aatcacatac gtgtcagctt atttattctg ataacttatc tattaacatt
     1441 agatcaatca gttaattact ccttcaagtt tgtctgtttt tccttacttt ttattttgtt
     1501 tataacaggc tctaacatct tatcaaagtt aaaatttttt ttcccaaatt cgcgaataaa
     1561 ctgtataact tctttatccg ttttgttcga ctgtaatatt accttagtat aaaaatctaa
     1621 aatatctgaa atattaatac ttgattcatc tgcaggtaca cgaaaataat attcataatt
     1681 ttcaaaatta tctaactctg tctctactcc agaaataatt ggaatacctc tcgctaagta
     1741 ctccttcccc ttaagcgtac tgttgtagta cacacctgat ctaaacctag ccaatgtatc
     1801 tacagccaga tcagtagtat caaaaacggt atctagctct tgtgagtcca tacctggaag
     1861 tactttaata atcccctcta aattactatt tgaaatcata ctcttatagc tgtttaaaac
     1921 ggtagtatta ccattaccta ttaaatttaa acgtatagcc caatctccac cttcagaata
     1981 atattgggca attcccttaa ttaatctatc ataaccatga aaagattgaa aattagct
//
LOCUS       DAEGIQ010000223         1979 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000223 DAEGIQ010000000
VERSION     DAEGIQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.236"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(325..528)
                     /locus_tag="KMT45_002928"
     CDS             complement(325..528)
                     /locus_tag="KMT45_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006329.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="KMT45_002929"
     CDS             complement(728..1744)
                     /locus_tag="KMT45_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBE7006330.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          559 a          458 c          308 g          654 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cgtaaatcca cgtcactaaa cattgacgta aatttactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgctcgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAEGIQ010000224         1922 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000224 DAEGIQ010000000
VERSION     DAEGIQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.237"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..987)
                     /locus_tag="KMT45_002930"
     CDS             complement(<1..987)
                     /locus_tag="KMT45_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBE7006331.1"
                     /translation="MLQHNVQKGWGRSFPLFFVGIVFFALFYLSTNIFADSNKVSFDA
                     QAILPDNQQSEASYYDLKVTPGATQDLSLKLKNTTAKQIKVVVEANNACTNKNGAIDY
                     SNHEAKLLGKPVFQDLISKSQTITLKPSEERDVTFQLKIPEKGFEGTILGGFYCYEDT
                     KGKEEKNEGFSLKNKFAYTIGVKLVCSDKKVEPGFALTKVKPGLENGYLTLFANLENS
                     EPVLKSKMDLHAVIKKKGEKEALHEFNQKVSFAPRTQFQIPFNLNNEPLKKGTYELGI
                     QLKDDSGKKWTLYKEFVIKGEDETLNKDAVEVNKENNDHSLFYILLVLCLIII"
     gene            complement(974..1633)
                     /locus_tag="KMT45_002931"
     CDS             complement(974..1633)
                     /locus_tag="KMT45_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBE7006332.1"
                     /translation="MKKTMITTAALGLLAVTASGSMVSADMISGTTNKTDVTLTEGSG
                     TDVTPVVPDDTHDDDPSTGDVGSLSIPFASNITFGQQEIQQGDTDYYALNKKPHVQVN
                     DTRGGAKGWTLGVTISPFIGENGKELTGAKMSLTNGRVVTKDNTSEAPKLANQTFDLN
                     EAYQDVMLAKEGQGAGAFAAVFEGEDGHNESVKLHVPRAGVEAQSYTADLTWVLSDAP
                     A"
BASE COUNT          551 a          396 c          306 g          669 t
ORIGIN      
        1 gataatgatc aaacaaagaa ctaaaagaat atagaataaa gaatgatcgt tattttcttt
       61 atttacttcc actgcatctt tgttgagcgt ttcgtcctct cctttgatta cgaattcttt
      121 atagagtgtc cattttttac ctgaatcatc ttttaactga atacctaatt cataggttcc
      181 ttttttcagt ggctcattat tcaaattaaa aggaatctga aattgtgtgc gtggtgcaaa
      241 agacactttt tggttgaatt catgtaacgc ttctttttcc ccttttttct tgataactgc
      301 atgtagatcc attttgcttt taagcacagg ctctgaattt tctaaattgg caaaaagagt
      361 caaatagcca ttctctaatc cgggtttaac cttcgttaaa gcaaatcctg gttccacttt
      421 tttatctgaa caaaccagct ttacaccgat tgtatatgca aacttattct ttagactaaa
      481 accttcattt ttttcttctt ttccttttgt atcttcatag caataaaatc ctcctaaaat
      541 ggttccttca aaacctttct ctgggatttt caattgaaag gtcacatcac gttcctcaga
      601 aggtttcaat gtgatcgtct gtgatttaga aattaaatct tggaatacag gttttcccaa
      661 taacttagcc tcatggttag aataatcgat tgctccattt ttattagtac aagcattatt
      721 ggcctctact actactttta tttgtttggc agtagtgttt ttcaatttta gtgataaatc
      781 ttgtgtggcg ccaggtgtca cctttagatc ataataactt gcttctgact gctggttatc
      841 tggtaaaatg gcttgtgcat caaaagatac cttgtttgaa tctgcgaata tgtttgtaga
      901 caaatagaat aaagcaaaga atactatacc aacaaaaaag aggggaaagc ttcttcccca
      961 ccctttttgg acattatgct ggagcatcag acaacaccca agtcaaatca gctgtatagc
     1021 tttgtgcttc tactccagct cgtggtacat gaagcttcac gctttcatta tgtccatctt
     1081 ctccttcaaa aactgcagca aaagctccag ctccctgtcc ttctttagca agcatcacat
     1141 cttgataagc ttcatttaaa tcaaatgttt gattggctaa tttaggagct tcagaagtat
     1201 tgtctttagt tactacccgg ccatttgtca aagacatctt cgctccagtt aactctttcc
     1261 cattttctcc aataaatgga gaaatggtaa ctcccaatgt ccagcctttt gctcctccac
     1321 gagtatcgtt tacttgtaca tgcggttttt tattcaatgc atagtaatcc gtatctccct
     1381 gttggatttc ttgttggcca aatgtgatat tagatgcaaa gggaatcgat aaagaaccaa
     1441 cgtctcctgt acttggatca tcatcgtgcg tgtcatctgg cacaactggt gtgacatctg
     1501 ttccagatcc ttccgtcaaa gtaacgtctg tcttattagt tgtccctgat atcatatctg
     1561 cagaaaccat acttcccgaa gcagttactg ctaacaaacc taacgcagca gttgtgatca
     1621 ttgttttctt cattttataa ttcctcctaa ataataatag atctaatttg atccaaaata
     1681 attttgacac gtccagtact ttttatccag ttcaactatt ttcccaaata aagaaaaaaa
     1741 ttagaaaaca aatgagataa ttcaatcaaa aagggactgt gacatgtagt gtcacagtcc
     1801 ctttttccga ataaacggtg ttctcttgcc aactcctcgg tcacaagtca caatattcaa
     1861 taaacatgag aagctgtttt ttgaaattgt tccttgtgac tcgaagatga acgggaggct
     1921 ca
//
LOCUS       DAEGIQ010000225         1909 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000225 DAEGIQ010000000
VERSION     DAEGIQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.238"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(647..937)
                     /locus_tag="KMT45_002932"
     CDS             complement(647..937)
                     /locus_tag="KMT45_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006333.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1145..1837)
                     /locus_tag="KMT45_002933"
     CDS             complement(1145..1837)
                     /locus_tag="KMT45_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBE7006334.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          519 a          366 c          294 g          730 t
ORIGIN      
        1 taaaagttat cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta
       61 cgatagtcaa caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta
      121 tggattgcag taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct
      181 tttaaaattg acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta
      241 actgtcgaac caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac
      301 tatgataatc acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac
      361 aattccacta cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat
      421 tgagcgaata ctaatagggc ttcgaattga tctgttcaat ctttaagcaa tatccctcaa
      481 atgagtttgg aaagtttagt gattcatcac aaatacttaa atacatgtag ttatatatag
      541 attgaggttc ttgtgagcgt atgctcgtgt gggttcaaat cccatcgtct gcatccatct
      601 ttccaaaaca agcggctagg acatttttgt ctcagccgct tgttttctag tttattcttt
      661 catcttgtcg gacctttttt cttgggtata gacttttctc atattctacc acaattttgg
      721 gtttccctaa cacatgatag gatctcacaa caaattgctt tctattatta taataacctg
      781 taacgactaa cttcatccct tcgtctactt cagctaggaa gttcaaacta tgggaagcaa
      841 tcaaacaatt gaccccattt aaactgaaac ggatcaacgg tctggcacta acttgcaaag
      901 tacggatctt atctactgtt ccatattttt tctccataac cttcacctca ataccattat
      961 acgaacatac attccctttg taaagaagaa actctgtttt atctttttga ctttttgtct
     1021 attacctttc ttaaaatcat attggcactt cctgcgaaga tgaaggacat gtactaaaaa
     1081 aaagaggcag cagtatagat tactgttgct tctttactaa tttaggttta cttgttaaga
     1141 aagattattt acgcaaatgt tcttcaacaa tttgctgaga tttatcagct gtatcttcca
     1201 cttcatcttt tgctttttct aacttttgaa tatattgttt caagcttgag ttttcagcag
     1261 ttgcttcgtc taatttcttt tgtgtatctg ttaactgagt ttctacattt gttttttctt
     1321 gtgtcagtgt tgcgatttgt tgattcaaac catttacagt attttgtaat tctgaaagtt
     1381 ttgcttgtgc ttcttgtagt cttgctgtcc aatctgcatt cagatgattg atttcatcat
     1441 ttttttggtt gatctcatct agtttgacat tgatttcatt ttgtttgctt tgtagttgtg
     1501 catccttgct gactaattga ctttctaagt tttgtttttc tgctgccgat tgattttttt
     1561 ggttttctaa ttcatttact tttgtttggt attcttggac atctgccttt gaatcttgca
     1621 atgtctgttc tttttgtgct agtcgagcgg taagatctgt aatcgcttgc tctttatctg
     1681 tcaatgtatg atcaagctgt gtcagattct cattgattgt atcaatatct gtctctccac
     1741 cccacaaaat aatttcattt gcgtaccgcg tgcctgtgta gccaattaga catagaaatg
     1801 caacgacaat cccaccagta ataaatttct ttttcatttg catagctcct taatccaata
     1861 cttttacata ctaaaagtaa gtgatctcaa acacaatgtc aagcgtttt
//
LOCUS       DAEGIQ010000226         1903 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000226 DAEGIQ010000000
VERSION     DAEGIQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.239"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(347..802)
                     /gene="tnpA"
                     /locus_tag="KMT45_002934"
     CDS             complement(347..802)
                     /gene="tnpA"
                     /locus_tag="KMT45_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBE7006335.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1045..1903
                     /locus_tag="KMT45_002935"
                     /pseudo
     CDS             1045..1903
                     /locus_tag="KMT45_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          605 a          370 c          355 g          573 t
ORIGIN      
        1 gatcatcaat acttacgtaa ctcaaaaata cttccaaagg tgttcgataa tccagtgatt
       61 ttctaggaat attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc
      121 ttggttgctt gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct
      181 ggacataagt ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag
      241 gacgggtgta tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga
      301 tttttaaaag accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg
      361 gttctacgta ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac
      421 ggatgtattt ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg
      481 accaaaagga tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta
      541 ccgcactacg acctttcaga taccccataa aactggatat acatattttt ggtggtactc
      601 gaacaagcat gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca
      661 tttcgcacaa cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt
      721 ttcgttgata ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta
      781 aacttttatc gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc
      841 actcactagt atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa
      901 cgcgcggttt tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa
      961 taaaagggtc taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct
     1021 aacttttgga ggtcggtatt ttttatggct aagtatactt ttgaacttaa attaaagatc
     1081 gttcatgatt acttggatgg taaaggcgga agtgattatc tggcaaagaa atattccatt
     1141 aaggctccat cacaagtgaa gcgctggatc aatgcctatc aagaatttgg tgaagagggt
     1201 ctagtaagaa agcgacaaaa gaaaaaatat tctgttcaat ttaagctgga tgcgatagag
     1261 ttatatctaa caagtgagct gtcctatcgt gaagttgcca acacacttaa catgaacaac
     1321 cctaatttga tcgctagttg gatgcgtcaa ttccgtgagg gtgggatcga tggactctca
     1381 aaaacgaaag gatgtccacc tatcttgtct aagaaaaatg aacccaaaaa taagaaaaaa
     1441 tctacaacta aagcaacgtc taaagaacgt gaacgcatcg aggagttgga gaaacgagtc
     1501 cgttcccttc aaattgaaaa tgccttttta aaagaattga ggaagctgcg aaaacaggaa
     1561 gcccaacaac gacgaatgaa gcaatcgcac gaatcatcgc aagcctccga ggaccgttta
     1621 aattagttga gcttctagaa actttaaagt tccctaaggc aatctttatg tactggcaaa
     1681 aacgattgga ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaagttcggg
     1741 agaagcacaa ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct
     1801 ctatcaataa aaagaaagtt cagcgcctga tcaagaagct aggaataaaa gtaacgacat
     1861 acacacgtaa atcacggcgc tacaattctt atcgaggaca agt
//
LOCUS       DAEGIQ010000227         1890 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000227 DAEGIQ010000000
VERSION     DAEGIQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.240"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..362
                     /locus_tag="KMT45_002936"
     CDS             <1..362
                     /locus_tag="KMT45_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006336.1"
                     /translation="TQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTPEEQIQ
                     RKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIVKAKVS
                     KRFIFNPKLEIYSVVIE"
     gene            687..1304
                     /locus_tag="KMT45_002937"
     CDS             687..1304
                     /locus_tag="KMT45_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7006337.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1345..1644
                     /locus_tag="KMT45_002938"
     CDS             1345..1644
                     /locus_tag="KMT45_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBE7006338.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1678..1845
                     /locus_tag="KMT45_002939"
     CDS             1678..1845
                     /locus_tag="KMT45_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE7006339.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
BASE COUNT          693 a          263 c          374 g          560 t
ORIGIN      
        1 tgactcaaaa taataaaaat aattctggat gtggaattat ctttatgttt gttatagttt
       61 tgggaatagt agtactttta ataactgtca agatagtaga ttactttgcg aatactcctg
      121 aagaacaaat acagagaaaa cttcctgagt ttgaaagagt accaccttat gatccaagag
      181 ataattcatt tcaagtaaaa aaattaaaag agtctccaaa tgaaatgtgg tttgaagtta
      241 cagttgaatc aagcgacttc aaagaaaaaa agaaagaata caattatatt gtaaaggcaa
      301 aagtatcaaa aagatttatt tttaacccta aattagaaat atatagtgtt gtaatagaat
      361 aatagtgtta aaaaaattgt aaaagttaaa gaagtcaaat tctgatgtga aaaataaaat
      421 ataccatgga agacataaaa gaaaaactgg ataactagtt tatttaatgg cagaccgtta
      481 aggcctgcca tttttatgta ttttagcgga ataaatataa tcatgcggga gtgtaaactt
      541 actgtttcaa aaagtatact ttttgacatg ccttgatttt ctttgtttat gtatggcata
      601 ataaccacat aatacaattt gaaacgtttc tgatttaatc tgattgtttt gaaacaattt
      661 tagtgaaaaa agggtagata aaaacgatga gaaaattttt ttatatccgg gtcagtagtt
      721 atgatcaatc gatcgaacga caactgattt ccgcgaagga attaggaatc cctgaagaat
      781 atatatttgt agaaaaagcc agcggcaaag attttaaacg ccctgaatat caattaatga
      841 aacggatgtt tcgaaaagga gatttactct atctacaatc attagataga ctaggcagaa
      901 ataaacaaat gattttagaa gaatggcgag aattaactaa ggaaaagcag attgatattt
      961 ctgtgttaga catgcctctg ttagatactg ctagttacaa gcaactagat gggttagaga
     1021 cgcttatctc tgacttaatt ctgcaattac tcagctatat ggccgaagac gagcggaaac
     1081 gaattaatag ccggcaaaga gaaggcgtag aagctgcgaa acaaaagggt gtaaaattcg
     1141 gcagaaagaa aattgcttta cctcctgaat ttcctgcgat ctattctgac tggaaagcag
     1201 gaaagataac tgcagtctat gctatggatc aattgggcat gaaacgaaat accttttatc
     1261 gacgggtgaa agaatatgaa cataccttgc accacttttc ttgatagata taacagctat
     1321 tagacgggaa gggagagttt gaaaatggac ttaacgaatc aaagggtcgt ttttactgga
     1381 acgctgcagc aaatgacacg aacccaagcc atcggtttgg ttcactcttt aaacggccat
     1441 tgtcaatcaa gtgttacttc aaaaacaaat ttcctggttt gtggtcagta ttctaagtcg
     1501 ttgtttgatg attctctcta tacaaaaaag gaacaaaccg cgcgggactt gattgcgtta
     1561 ggtcatgaga ttaccatttt atcagaagtc gaattttatg cgttaattag tcaacagttg
     1621 aaagaatggc aacggtattt ataatcgtta gtaaaattta caaggaggaa aatttttatg
     1681 aagcgaaaat cttatgatcg ttggggcatt gaaataacta gttttaatcg aaatattgct
     1741 tttcacgtgg agtgtgactg tggggaatta gcaaaaagtg tttatcaaaa aaattacttt
     1801 tactgtccta aatgccatcg ccgatacggg gtacatggaa catagaaacg aaatagtgcg
     1861 gtagaaaagc gaatgaatgt agtatatttc
//
LOCUS       DAEGIQ010000228         1882 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000228 DAEGIQ010000000
VERSION     DAEGIQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.241"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..183
                     /locus_tag="KMT45_002940"
     CDS             <1..183
                     /locus_tag="KMT45_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006340.1"
                     /translation="QHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKR
                     KNEIYYEQQLSQKERV"
     gene            244..1713
                     /locus_tag="KMT45_002941"
     CDS             244..1713
                     /locus_tag="KMT45_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBE7006341.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1724..>1882
                     /locus_tag="KMT45_002942"
     CDS             1724..>1882
                     /locus_tag="KMT45_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HBE7006342.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVEL"
BASE COUNT          709 a          292 c          399 g          482 t
ORIGIN      
        1 cagcataatc aagtaatttt aaaaggaaaa agttatggaa aagagatcca aacggcgtta
       61 ccttcagaag aatttgcgct aggaaattca ttccgatttt cacttgagac agtgtcagaa
      121 gtggcaaaac gaaaaaatga aatctattat gaacaacaat tgtcacaaaa agaacgtgta
      181 taaaacaagt aaaaaagact atctgaaaaa cggatagtct tttttctttt gggaggtttt
      241 aaaatgaccg agaaaaaaat gagtcttatt gatcgatgta aacagatcga tatagtggat
      301 tttgctcgaa acaatggaat ggctgtagtc aataaaggac gagactatcg cttagaagac
      361 catgattcat ttgtttttga tcgaagaaaa caacgttttt attggaacag tcaaaatatt
      421 agtggcgaca ttattgaatt agctaaactt tttttcatag acaaagagat ccaagaccct
      481 aagcaacaat ttaaagcagc cactgatttt attctaaaga atgaagacaa aacagaacga
      541 gtggaaaatc ttcattttga gacagaaaag tacaaagatc accccgttga ttatcaacca
      601 ctcacagaaa aaggaaggaa ttatctgaag gaagaacgaa aactaccaga gtggctgatt
      661 gattatgcag aaaaagaagg gttgattgct gaactaaagc cgaaacatga acggcaaaat
      721 tttttagttg gagacgaccg tttagatcat gcggttgctt ttttatggaa agatccacag
      781 acaggagaga cagtaggggc aagttatcaa ggaacgatcg tcgattttaa tcgtttcggt
      841 aaacggggaa cgtataaaca cattgacaag aatcctacgc caaatcacgg atttaattta
      901 aaaataggcg atccgaagca tttaaaattc tttgaaagta gtattgatct gttaagttac
      961 gctgcattga atcgggagaa actacaagat gcctggttag tttcgatgga cgggttaaaa
     1021 catcatgtaa tcagtcatta cgttgaagag tcgatttctg aattaagacg aaaacaaaca
     1081 tttcctcaat cgattgaaat atgtgtggac aatgatcgtg caggacatat tttttatgaa
     1141 aaagaacaga tgaagggaat cgttgatcct tttaccaata agaaaatccg ttgtgagcga
     1201 ggaattccaa atgattggca agtacctaaa gaatacaaag ctacttatga agcagtcgca
     1261 aaagaaatga gtgttgaacc tgaagcaatt atggccatac acaaaactga aactaattta
     1321 cagctgacaa atcaattggt gtctgctcat gatgttcaat ctacgtttgg aaagatgttg
     1381 gcgaagggcg aaccagtaga aacaattgat ttgaaagaag catgtaccac ggtcgcgaag
     1441 gaactgaaag tatgtgaacg cgcagatgga acatacaact ttgatcgttt ttatagtaga
     1501 aaagccaaca taaaagacgt taatgcagga atccttcttt cctataaagc agaacaatac
     1561 tacaaaggct ataaaaagca tgaacatgaa tttgtacctg aagtgaaaaa ggattggaat
     1621 gatcaattga agcatgagat ccagcaacaa gaaatcagaa agcaaaaacg tgcgatgttg
     1681 tttcaacaag gcagacaaca agaaagggag tgaagtcgtc actatgaaaa ccgtcatttt
     1741 agcagaaaaa ccgtcacaag cgaaggctta tgccgatagc ttttctaaag ctacccgaaa
     1801 agatgggtat tttgagatcc aagatcgatt gtttacagga gaaactgtga tcacgtatgg
     1861 ttttggtcat ttagtcgagt ta
//
LOCUS       DAEGIQ010000229         1847 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000229 DAEGIQ010000000
VERSION     DAEGIQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.242"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            64..1087
                     /locus_tag="KMT45_002943"
                     /pseudo
     CDS             64..1087
                     /locus_tag="KMT45_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1430..1686
                     /locus_tag="KMT45_002944"
                     /pseudo
     CDS             1430..1686
                     /locus_tag="KMT45_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          696 a          241 c          332 g          578 t
ORIGIN      
        1 tttttttcga tttttcaagt tatctaatgc aattatattg atatcattag gagctgcatt
       61 gttatgaatc caacattgat tactaagaaa gaattactaa aaaaattaga tatttctact
      121 ggtgtattag ctaatttaat tagaaatggc atgccaaaag agggagaaat gtttaatctt
      181 gataagatta ttacatggcg agaaaattgg tctaaaaaca tattaggtga gttagaagtt
      241 ggtagagtat atactaacaa agaaatttca gagaagttta aatgttccaa acagggtgga
      301 atgagaagat ctcatcaaac taatactctt gtactgttta gtgatcaaac aggaagtaat
      361 gtttataaag ataagtggtt aaatggtatt ttacaatata ccggtatggg gctaaaaggg
      421 gatcaagtat tggataagaa tcaaaataaa gttttagcca attcaaaaag taattttgta
      481 aagattcatt tatttgaaac atttaaacca aaagaacaca catatctggg agaagtatat
      541 ttagcaggac aaatatatac tgttaatgaa aaagatagtt cgggcaacag cagaaaagta
      601 tataaatttc cattagcttt aataaatcaa gagcaattga ttgaagataa agatatataa
      661 ccaggaagaa aatcaaacta gacatattcg taatttatct aatgctaaat tagaggaaga
      721 agcaagaaaa gttagtaatt ataataaaaa aatggctgca aaatatgtta acgaagagga
      781 gttttgcttc tcaaaaacaa aaatatataa acgtaatccc ttcgtatctg aatatgttaa
      841 acgacttgca aaaggtattt gtcaactttg ccaagaaaaa gggccattta taaaagacgg
      901 tgtaccttat ttacattgtc atcatataga atatttgtcg caaggtggaa aagatgtgat
      961 tgaaaactgt atcgcgttat gtcccaattg tcatgctaga attcatgaac ttgagttacc
     1021 tgacgataaa gaaaaattgc tgaagatcgt tgaaaaaaga gaaaatagct tttctaaaat
     1081 accgtaattt atttttatag gacaaataag ctaatgtgag ctatgccttc tatataagac
     1141 taagttctat tataagtaac gaagtaattc tcgttccttc ctgtagtaat attttttaaa
     1201 taacctatag gcttcttcaa aagtacatcc tccagtaaat tctaagtact ggaggattct
     1261 ttttctagtg ttgctttact catgaaaaag tagttggcta cttcatcaaa atttctgaac
     1321 ttttttaatg tataataaag agctcgatca tttactacaa ggatgctagc tcctacattt
     1381 tcacagtttt ttagcaagta aatttttcta aaatcacatc aggaaatgca tgagattctt
     1441 taagagaggc aattataggg ctatgattac ggttcgtgta agtgacgcca aaaaagaatg
     1501 gctttcttat atggctgaat tttatgggat tactttatct gaacttctaa aaaactattc
     1561 gatggaacaa ctagaagatg aatacaatcg tcaaacggct gaaattgccc ataaacattg
     1621 gataagaagt gggaaaacaa cagtattcat ggaagaatcc ttaatgaatt tggtgggctg
     1681 gaatgacctg ccgtttaaaa ttcacgcccg aaattcagaa gcagctctga ttggcaaata
     1741 cgtgtattgg tagagataga agataatcaa ttcgttgtta tcgctattca agtagggcac
     1801 cgaagaaatg tctatgatta agagactgtt tatttttaca aaaaaat
//
LOCUS       DAEGIQ010000230         1831 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000230 DAEGIQ010000000
VERSION     DAEGIQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.243"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(12..1337)
                     /locus_tag="KMT45_002945"
     CDS             complement(12..1337)
                     /locus_tag="KMT45_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBE7006343.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
BASE COUNT          540 a          366 c          286 g          639 t
ORIGIN      
        1 gatcattgta atcaatcatt tttaattccc ccatttccgc ccgcatgacc accaacaagt
       61 gtgcttctag taatgcttct agcgccttct aacattgaag aagcatgtac aatcgacgta
      121 aaaccatatt tttgccgaat cttgtcaata attttatcta aattttcttc gtcaatttgc
      181 ttttgtggat cactaaataa atccagctga agcgaatcgg tgtacacaag cttgctatag
      241 gttattccaa catgccttac ttcttgtcct tcataatact tatcaaacaa gaaaagacaa
      301 taattctgca attcctttgt attatctgtg atagggatct tcatctggtg tgaaaaccct
      361 ggtctcgttt ctaagatcga cgtgccaatg tgtaaatgga cacaacctgt tttacagtta
      421 tgctgcctaa tgcgtatcgc tacttgttcc gccatttctt ttaccaccag ctcgatttca
      481 tttcttctag tataatctcg tggtaacact tgtgaatttc catatgattt ttcctttgtc
      541 ggttctggtg ggattgctat atctgttcga tcgatcccat tagcatggaa gtacagctgt
      601 aagccaatca cacctagtct attcttaatc gtataaggat tccagttagc aagatctcta
      661 atgctcataa tccccatctg attgagtctt tttttcattc gagagccaat tccccaaaag
      721 tccgtcattt caggaatatt ccatactttt tcaggaacgt tttcgtacgt ccattccgca
      781 acaaatcctt cattatgctt tgcttcgttg tctaaggcta gtttagccaa aaggggatta
      841 tctccgacac ctactgtagc aatcaatcct agctcttctt taatattctc ctggatcatt
      901 tgagccagct tctttctccg ttgacttcgt gttccttcag tcgtaaaaag attcagtgat
      961 cgggtcactt ttaagattga ttcatcgatc gagtaaatca gtagatcttc atcagccaca
     1021 tatcttctga aaatattatt tacctgcata tttcgcttga tatataggtt catacgtggt
     1081 ggaacaacgt gtagtgtttt aggaaatggt tgtggtaagt cacgtggtct actcacattt
     1141 gtaataccat accgcttttt tgcttcagga gaagaagcta atatcaatcc tgaaccagta
     1201 ttatcagctc gactcataac aacaagttct gttgttaatg gatctaaatt tctttctata
     1261 cattcgacac ttgcgtaaaa gcttttcata tcgattaaaa agtaatcatt tactggctct
     1321 tttgaataat ccatcataaa taacactcca ttctttcaca ttatacaaac atatgttcga
     1381 tattgtaaag aagaaatagc taaatacata aaaaaagaag cataggtatg ctatcaccct
     1441 gcttcttctt ttgatttgta tgatcaattt attctatcac tttactaatt aatttgaaaa
     1501 tctaataaaa actctcactc taaaagagtg agagactgca aattagagta cggacttttt
     1561 tggagttccg ttctttaata ttaacaaatg aataaaatcc ttacaacatt tgagaataag
     1621 ttatcagtag acacttacaa aaaattctca tactatttcc tccatcatta gtttttgtct
     1681 atataaggtt taccctaaat agacactggt ataacattga aaaatatggc ttcctcgtgt
     1741 atgattaggg tgtattttaa atagttgcag aaagtaggta aaagttttga gagttgcata
     1801 catacgtgtc tcatcgatcg atcaaaatga a
//
LOCUS       DAEGIQ010000231         1795 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000231 DAEGIQ010000000
VERSION     DAEGIQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.244"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            173..370
                     /locus_tag="KMT45_002946"
     CDS             173..370
                     /locus_tag="KMT45_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006344.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            476..958
                     /locus_tag="KMT45_002947"
     CDS             476..958
                     /locus_tag="KMT45_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006345.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1016..1612)
                     /locus_tag="KMT45_002948"
     CDS             complement(1016..1612)
                     /locus_tag="KMT45_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBE7006346.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          559 a          275 c          361 g          600 t
ORIGIN      
        1 tgaaaaaaat tcttctaaat acaacagctg ttttaattgt gaaaacgaaa acagtacgct
       61 ataatgcctg taaggttgat ttttaactgt aaataaaaat caatgagtta aatctccaaa
      121 gaaatagcag tggtaatgtg gagaaaagtt ccagcagttt aggaggattt tgatgacgga
      181 agaaatgatt aatttaggag aacaatatgc atgcaaacca attggtttta caaaaacggt
      241 cattggtgaa gttgtatcaa aaatgacaaa ctgcgccgtg gtcaaagttg cccaatgtgc
      301 tgcagaggac caagaattat tagatgaaaa agcaagcatg gttgttgcaa aatatgatac
      361 atttgaataa agaaaacaat tggacgtgcg gcgtgctgca agtccaattt ctttgagaag
      421 gtaaatcagt attcttttcc tattttctgg agaagaaaaa gcgaggcgag ttgtcatggt
      481 aacattcttt acggttctat tgtttttatt cgttgttgtg atgtttttat ttctttggct
      541 ggttcgaaaa gaaattattt atcggactgt gcgaaatcga tgggtctact tggtgatacc
      601 cttcttagtg tcactagtca tttggtatac actcatcagt catcctacag cagatgaatt
      661 ggcaaaaggg atcttgtcag caatgatttt tattagtttt ttgttggaca gtagaggaat
      721 cacagaagaa ggacttgttt tgaatagctt tgacaaaaaa ggtgttcctt tttctgagat
      781 caataaaatt gtattatatc agccaaaagg ttctaaaatc gtaaaaatga attttttccg
      841 aaacggatgg cgaggaccga tgctgaaatt ttctgcatca ttagaagaat tagttccatt
      901 tttgtcccaa cgcttaaacg atgaggcaga gattgatatt atgattgatc cagaataaaa
      961 acagaggttg cgacaagatg tttgtcacaa cctctgtttt ttatttcaaa taaaattatc
     1021 caaatacgcg gaagcgatcg gcatcgttca tgtattcttt ttccccagcc ggtgtttctg
     1081 gtgaaccgaa tactaattgc gaacgtagtt tccagtttga agggatattc cattcttttg
     1141 caactgcttc gtcaataact gggttgtaat gttgcaagtt tgcgcccaat cctttatcaa
     1201 ctaatgctac ccaagtatta gctgttgcga ttccgtttga ttgttctgac caatctggga
     1261 agttgtctgc atataattca aattgttctt gtagaccttt taccacgtca gtatctttga
     1321 agaataatac tgtaccataa ccatttttga aaccagctaa tttgttttgt gtatttggga
     1381 aagcttctgc tggagtcaat gggcgaagtg cttcttcagt gatttcccaa agtttttcat
     1441 gtgcatctcc gaaaaggata actgcgcgtg ttgattgagc attgaaagca gttgggcttt
     1501 ctttaattgc ttctttgatc aaagttgtta attcttcatt agaaagtgaa acattacgtc
     1561 ctagatgata gattgaacgg cggtttttca aagtgtcagt aaaagttgtc ataataaaat
     1621 ttctcctttg atttgcgttt tgcatttttt taattgatta cgaaatgaag tatagcacct
     1681 tacaaaaagt aagtaaacag atatgctcac aaaaaataag cgaagttcaa aaaacaattt
     1741 taataagaaa aatcgttatg taaggatttt cttagttatc gctttcattg ttgtg
//
LOCUS       DAEGIQ010000232         1789 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000232 DAEGIQ010000000
VERSION     DAEGIQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.245"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            19..570
                     /locus_tag="KMT45_002949"
     CDS             19..570
                     /locus_tag="KMT45_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7006347.1"
                     /translation="MKKFGAERIFIEKQSGATITHRPVFQEALDYVRDQDIFIVEAID
                     RLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGNPLLDKFIKDLIIQILAMIAEQ
                     ERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQRRLVYKSIVEDLKNGVAIAKI
                     AKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(735..1559)
                     /locus_tag="KMT45_002950"
     CDS             complement(735..1559)
                     /locus_tag="KMT45_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006348.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          595 a          312 c          254 g          628 t
ORIGIN      
        1 caacgccaga ttgaagaaat gaaaaaattc ggtgcagaga gaattttcat cgagaaacaa
       61 tctggcgcaa cgattactca tcgacctgtt ttccaagaag cattagatta cgtccgagat
      121 caagatattt ttattgtgga agctattgat cgacttggaa gaaattatga cgagatcatc
      181 gcttcagtca attacttgaa gaagaaaaat gtgaagctga tcattactag tttaccgatc
      241 atggctgaag caattggcaa tcctctcctg gataaattca ttaaggatct gatcatccaa
      301 attttggcca tgatcgctga acaagaacgg actgaatcga aacgtcgcca ggcacaagga
      361 attaagatcg caaaagcaaa cggcgtctac aaagggcggc ccaaattata cagtgcagac
      421 gcaaaagatc cccaacgtcg ccttgtttac aaaagcatcg ttgaagatct taaaaatggt
      481 gttgcgattg ctaagattgc taaagactac aatgttacga gacaaaccgt gtatagaata
      541 aaaaaagaca gcatggtcaa tgacaaatag ttgatcatgc tgcttttatt atcttctcat
      601 ctttaatttc ctacatataa ttactatcta taaaattaag tcttactcag aacagataaa
      661 gcataaaatt ccaacaaacc attttttact taactaaata aagagtctgt taatataatc
      721 ttcacttatt tacgttaatc ccctactgta tttgttggtt ccttatctat attttctaca
      781 aaatccttat tatcttgtga tttagcttcg tataattctt tgtatgtcga cacattggtt
      841 atactaattc ctatagctag ttcttgagac tctacttttc tctctagctc ctttgacaaa
      901 gtgatttgag atttatttgg ttccgttgga gtcaattcta tagattcttc ataaaaatct
      961 ctatacataa agctatcatt aattttataa aaaaaattaa atgtttttag cactgccata
     1021 taacattcaa aacattcttc tatagttggt ttacttcctt gaggagaatg aaccaattta
     1081 ttacgttttt ctatgaaatt tttaatttcc tttcttattt ctttcggaaa ctcaatatta
     1141 aagtaccctt taaacatgct ccctaataat ttgtctacag ggggagattg taaattctca
     1201 aatattagag ttaaatcagg tttctcttgt tgataaaatt cctttacccc tatttcacag
     1261 gctgtaatta tcgaaataaa cctatctcta gtatttgata tatttttact tcttttaagg
     1321 agtataagag aaaaggggat actttcgtgt ccatgcatgt aattaactaa ttgttgagct
     1381 tcagaagtta tatctctaat tatatagtta acggaattag gaagtgtctt taactctaat
     1441 gaaatttcta tccaattact atcttctaat ctgtatatgc tcgaagtata atttgattcg
     1501 tcagcaagca tcgctacatc ccaaacactg atcataaaat cttttaaacg ttcaatcatt
     1561 tcagcaatat tgctaaaatc agctaatttt tctttagaat aaaaaaaaac atttatatca
     1621 gaatcaataa attctatttt tttattacta tatcgtcatc attatcttga ccatttatgt
     1681 ttattgttcc ctgagtgggg agtacttctt tcaaattcaa gttaaattta tattccatta
     1741 cttctcctcc aatatataaa aattacttaa ctgtatccaa atttaatat
//
LOCUS       DAEGIQ010000233         1783 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000233 DAEGIQ010000000
VERSION     DAEGIQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.246"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1161)
                     /locus_tag="KMT45_002951"
     CDS             complement(<1..1161)
                     /locus_tag="KMT45_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBE7006349.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIA"
     gene            complement(1263..1616)
                     /gene="tnpB"
                     /locus_tag="KMT45_002952"
     CDS             complement(1263..1616)
                     /gene="tnpB"
                     /locus_tag="KMT45_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBE7006350.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(1606..>1783)
                     /locus_tag="KMT45_002953"
     CDS             complement(1606..>1783)
                     /locus_tag="KMT45_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006351.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          494 a          374 c          320 g          595 t
ORIGIN      
        1 agcaataaat aatggacgca gttcttgttg ccgacagctt aatcgcttct caggagaaaa
       61 gtctttccat ttcttttcta ggacaaacca tttatccaat tgtttgactg cttgagctgc
      121 gggaatatcc gtgtttttct ttccttttgg aattgcttcg aaaaatttcc gccgaacatg
      181 cgcgaaacaa tagacaagtc gtacactttc cacggcatta tacccgctat agccgtcaca
      241 gtgtaaatac cctgtaaact ccttgagaaa agttttcgga acatctccag cacgactttc
      301 gtcgtggtga taataaacga tgggatgctg actgtctttt cctgtacaaa actgccagta
      361 ataggtatct gttttggcac tttcaatcac acgataactt gtttcatccc cgtgtagaat
      421 ctcttcttga ttaagataga ctcttaatcg ttctcctaaa gggtctaacg cgttttgcac
      481 tgctttaata tgccaattgg tgatattatc tctcgaaata tccaacccat gtgtttgttt
      541 ccaatagttc tcttgccgat aggcaggaac tttctgacca aatttcatcc ggatcgtttc
      601 cgccactaaa ctattggaac ctaagctatt agaaatcaaa ggtttaggaa cgatcgcttt
      661 tttaaaagaa gaaagtcctt ctttttcgca gcttttacaa caataggtat gttgataatg
      721 ggtgagacaa tccaacatag cctgatggaa acgaatttct tctcgtactt ttgtccgtcc
      781 gatatctttc atctgtgaac cgcaatgttc acaaaattgt tcttccaaag gaaggagatg
      841 ttctttctct gttataggta agtgtttgat tttgtctgct ttcgttccta caactcgttg
      901 atgtcctttt actggaataa tggtggcagc tgcttctgga acacttacag aaatttcttc
      961 atcactaaaa agattcaatt gcccattcgt ttcctcaaag agagtctctt tagagcgtcc
     1021 aaataatttg ttggttaaat aattgacctt ttcattcaat agttggattt cagttgtaag
     1081 attctgaatg gtttgttgtt gtgcttgatt ggttttatgg gcttcttgaa gcaactgaag
     1141 caacgtatct gtagtttcca aaatttttca ccaccttgaa tcgaactgat tttgttacag
     1201 aataccacaa gaaacgcaca agaaataggg gttcaacaca ttttttgtgt taacccccca
     1261 gcttaaaaaa ctcctttttt tcctggcaaa atagctcgag gttgatcgat acttaatcct
     1321 tctaacaacc atcgaacctg ttggttcgtt aaggttcgaa cttcgtcttt cgtccgaggc
     1381 cattttaact tgccgttttc tatccgctta taacagagaa gaaaaccatc accgtcccaa
     1441 tagagaagtt tgaaacggtc ttgacggttc ccgcaaaata aaaaaacggc atcttcgtaa
     1501 agctcgagtt gatactcttg ttgaataaga ctggcaagac catcaattcc ttttcgcaaa
     1561 tctgtttttc cgcacacaat ataaatatgt ttggtttgaa gtaaatcata aagcattgga
     1621 caacagttct ttcaaaatag cttgaagaat ataattgtta ataccgttat agatcaaaac
     1681 ttcagaacca ttctctcttt tgatgcgcat cgctaacttg gcagccgcgg gtttaccttt
     1741 ttctggtgca ctcttttcgt ttcctaattg gacaggaaca atc
//
LOCUS       DAEGIQ010000234         1691 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000234 DAEGIQ010000000
VERSION     DAEGIQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.247"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..862)
                     /locus_tag="KMT45_002954"
     CDS             complement(<1..862)
                     /locus_tag="KMT45_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBE7006352.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYI"
     gene            complement(1021..1287)
                     /locus_tag="KMT45_002955"
     CDS             complement(1021..1287)
                     /locus_tag="KMT45_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7006353.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1277..1633)
                     /locus_tag="KMT45_002956"
     CDS             complement(1277..1633)
                     /locus_tag="KMT45_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE7006354.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          487 a          323 c          205 g          676 t
ORIGIN      
        1 aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt aaaattcccg
       61 atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc cttaaattgt
      121 tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa aagatagaat
      181 tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat tctattggat
      241 caaaaatatc taagttgtca tcaaaatctt tatcagttcc ggtaactttt ttgttaatta
      301 ctgaaggtgt cttgtaagaa atagaatcta aatcaaagcg ttttgattct aaattatttt
      361 gtgctttctc aattaaataa ttcacttttt ttgtgagaaa ccttctgata ttgtctttgc
      421 tatatgttcc ataaaaaccc ttatcttcta aagagtccct gtacttctta acttctatat
      481 actcttcagc caaaacttgt ggaactggtt catctaagaa aaaaacccct acctcgcctt
      541 tgctattttt ataaaagtaa tcaatttctg ctatagttcc cgatctataa ttatcaactg
      601 gagaacccaa cttatggcca aaaatcgcta ataatatatc actattttct acaatttgtt
      661 tattaataac actttgccca gagctattat ttatataagt aggaatcgca ttcgtttccc
      721 atcgaattgg catcaaaatt acttttgtat tatctgtatt ttctatattc catctatata
      781 tggtttcttc tacttcattc ctctttgctg ctatatcacc tggtgatgct ataaatactt
      841 ttattagatt tatggttttc atttattgta cctttctctt tatgtctctt taaagtaatt
      901 tttgtataaa atttattgac ttttacacta taggacaact tttatctatt tagtacaaaa
      961 aggcccacta aactaattga ttagttagga gcctattaca ggtatatttt caactcgctt
     1021 ttaagcaaat tctactacca gctttttgcc taatgcccta gcgatatcat caagtttatc
     1081 gaaagatacg ttatcccctc tctcaattcg agcaatagta gattgagttg tatgtgcttt
     1141 ttgtgcaagc tccgcttgag ttaggcctgc ttcctcccgt gcatgataaa gagcaaccgc
     1201 agtttcgagt tttcttcctt cacggtcata tgcttctgca aaatctttgt ctttttgctt
     1261 ttcttcaatc aattttttaa tcatcatttg tgaaccactc ctctctaagt tcttttgcat
     1321 gtttaagttc acttattggt gttttctccg tttttttagt aaatccatgg gtaatgacat
     1381 accttccatt gaccacatgg aaatagatag ctctttggat gtttgatcct accttacttc
     1441 ttaattcgta caaattagtg tctaattttt tcacccattt caaccgttga gctactagca
     1501 tcccattctt ttctgtttct tctattgtac gtaaaagctt cgcactatct ttttttggta
     1561 atgtttccaa ccattctaaa aattcatcat gaccattagg tcttttaaat gtttcaaaag
     1621 atattttttc catacccaat tatatcatat attgatttta tattatagta tataacataa
     1681 aaattcgaat t
//
LOCUS       DAEGIQ010000235         1673 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000235 DAEGIQ010000000
VERSION     DAEGIQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.248"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(97..318)
                     /locus_tag="KMT45_002957"
     CDS             complement(97..318)
                     /locus_tag="KMT45_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006355.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(416..661)
                     /locus_tag="KMT45_002958"
     CDS             complement(416..661)
                     /locus_tag="KMT45_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006356.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            901..1515
                     /locus_tag="KMT45_002959"
                     /pseudo
     CDS             901..1515
                     /locus_tag="KMT45_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          571 a          285 c          256 g          561 t
ORIGIN      
        1 tgcttaaata aatttgtatc ctgaaattta ttgtaatatg aagctgtatt ttacatatag
       61 taaccaaaga gttaataaaa taaaaagagc gaaccattat agatcagctc ttttttcatt
      121 gctgttttta atcttatcta gaatcacttc aaatgtttca aaggtattta catctaactg
      181 acataaccaa agcaagtact tcttttcttt gtaagtccaa tcaatattac ggtaagagga
      241 cattagatta tggtaccttg ctctttgttc aactgaagcc aattcccagc gtttttcaaa
      301 tattttttct tcttccatta ttgatccctc gcaagacgct acctcaaact taaataatgg
      361 gcagcgaaag tctaattcct tagatctaag tatagcaaac aaatatgaaa aaaacttaga
      421 aggtagtaaa agttcttccg tcttttgctt tgcttctatt cttaatgtgt ttcgttatcg
      481 gtgccctaca aagattttca ttaattacac actcatttat tgttgtgtat tttctgacat
      541 ttctctcttg atcaattatt ttaattccat gggcatcctg atagttcata ggagaggagc
      601 ggctatcaga aatagttgca cctatctctt gaacttcttt tacaacttct ggaaaatcca
      661 tccatcctgc ttcagctgtc aaaagtaata atctataata tttatctttc cttttcaatc
      721 taagaaatcg tcaccccatt ctttttcttc tattataaac tatttaactt ttagaattca
      781 aaaaccgttt gtctatttag agtttaccct aaatagacaa aaatataaca ttgaaaaatt
      841 tagagttcta acgtatgatt agggtgtatt ataaacgtta tagaaaagta gggaataaat
      901 ttgaaaattg cttatattag agtttcatcc atcgatcaaa atgaacaacg tcaaattgaa
      961 gaaatgaaaa agtttggcgc tgaaaaaatt tttatcgaaa aacaatctgg agcgacgatt
     1021 agtcatcggc cgattttcca ggaagcttta gattttgtca gagagcaaga tatttttatt
     1081 gtagaagcca ttgatcgact tggacgcaat tatgacgaga ttatcgattc agtgaattat
     1141 ctaaagaaga agaatgtgcg acttattatc accagtttac cgatcatggc tgaagcaatt
     1201 ggaaatcctc tcctagataa attcatcaaa gacttaatca tttaaatctc agccatgatt
     1261 gctgaacaag aacgtactga atcgaaacga cgccaggcac aaggaattaa aatcgctaaa
     1321 gcaaacggcg tctataaagg tcgtcctaag ttatacagtg cagacaccaa agatcctcaa
     1381 cgtcgccttg tctataggag tatcgttcag gatcttgaga atggggttgc tatctcaaag
     1441 attgcaacag actacaatgt gactaggcaa acaatttacc gaataaaaaa agaaatcgac
     1501 caactaattg tatgatttct tttttgaatc acgtaattat taattagctt tgtcatcaat
     1561 acagacaatc attttagatt catctagcta agttgattac atttatgtta aaaagaattt
     1621 agttaacata aatataattg atgcataaat cgtccttatt taaaggcatt taa
//
LOCUS       DAEGIQ010000236         1664 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000236 DAEGIQ010000000
VERSION     DAEGIQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.249"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            131..505
                     /locus_tag="KMT45_002960"
     CDS             131..505
                     /locus_tag="KMT45_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBE7006357.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="KMT45_002961"
     CDS             complement(619..1248)
                     /locus_tag="KMT45_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBE7006358.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="KMT45_002962"
     tRNA            complement(1382..1454)
                     /locus_tag="KMT45_002962"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAEGIQ010000237         1632 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000237 DAEGIQ010000000
VERSION     DAEGIQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.250"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            290..697
                     /locus_tag="KMT45_002963"
     CDS             290..697
                     /locus_tag="KMT45_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006359.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            711..1112
                     /locus_tag="KMT45_002964"
     CDS             711..1112
                     /locus_tag="KMT45_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006360.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1114..1485
                     /locus_tag="KMT45_002965"
     CDS             1114..1485
                     /locus_tag="KMT45_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006361.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          612 a          209 c          283 g          528 t
ORIGIN      
        1 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
       61 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      121 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      181 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      241 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      301 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      361 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      421 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      481 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      541 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      601 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      661 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      721 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      781 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      841 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      901 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      961 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
     1021 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
     1081 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
     1141 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
     1201 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
     1261 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1321 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1381 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1441 ctgatgaatc gagaagcaga aaaaataata aacaaactca cctaatgaat ttcaagcgag
     1501 tttgtttgtt atttttttat ttaatctttg tatctttctg gatcaacgaa agtatacttt
     1561 atatagtcat aacgccgatg atcgctacgt gcgtccggca cgtcagtcac gatatcaaac
     1621 aaaaagtata ca
//
LOCUS       DAEGIQ010000238         1598 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000238 DAEGIQ010000000
VERSION     DAEGIQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.251"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            149..1318
                     /locus_tag="KMT45_002966"
     CDS             149..1318
                     /locus_tag="KMT45_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBE7006362.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          573 a          257 c          259 g          509 t
ORIGIN      
        1 atccatagtt aatttatcat aatatcccat attttaaaat aaaagatagc gtaaaatatt
       61 ttgtgtaaat agaaatttag ggtagaaaaa aaagtccatt ctgtaaaatt aaagttacca
      121 cacagaaatt ttataggagg actttctcat gactaaattt actatagaaa ttatgcaaac
      181 actactcaat aaaggggatt tagatgaatt atttcaggat catttagagc gagcgattaa
      241 ttcacttcta caagcagagc taactgcttt tttagattac gaaaaatatg atcgaaatgg
      301 attcaattca ggaaattccc gtaacgggaa ttattcacgt acatttaaaa cagaatatgg
      361 cgagttgaat ttaactattc ctagagatcg gaatggcgaa ttttctcaac aaacactacc
      421 agcatataag cgaaccaatg attcattaga aacgaccatt atccaattat ttcaaaaagg
      481 cattactatg gctgaaatct ccaaattaat tgaaaaaatg tatggtcacc actatacgcc
      541 acaaacaatc tccaacatga gtaaattggt ggctgaagat gttttagctt ttaaagaaag
      601 aacgttagaa gccaattatt ccgttatatt tatggatgcg acgcatatcc ctgtgaagcg
      661 acagactgtt tcgaaggaag cagtgtatat tacgattggt attcgtttgg atggaaccaa
      721 agaagttctt gggtttacta ttgccccaac tgagtctgct tatatttgga aagaagttct
      781 tcaagatctt agaaaacgtg ggttagaaga agttttatta gtagtgacag acggattaag
      841 ctgtattgaa gaaagtatcc atagtgtgta tccgaatgcc caatttcaac aatgttgtgt
      901 gcatgtatct agaaatatcg ctcataaagt tcgtgttcga ggtcgaaaag aaatttgtga
      961 ggatttcaaa ttggtttacc aagcgaattc aaaagaagag gcattggatc acatcgactt
     1021 tatgactagg aaatggaaaa agcagtatcc aagagtcgtc aatttactct tgaatcctgc
     1081 cctattaacc ttttataatt tccctcacgc catcagacga acaatttatt cgacgaacct
     1141 gattgaaggc tttaataagc agctaaaacg atatactcga agaaaagaac aattccctaa
     1201 tgaagaatct ctagagagat tcttcatttc tcaatttaat caatataacc aaaaattttt
     1261 aggtagaatt cacaagggat ttaaagaaat tcaggataca ttagagtcga tgatttaact
     1321 gtaattaaca gaatggattt tccatttaca tataattctt gacgctacca taatctaaat
     1381 aaccatcata ttcatctact acttatataa ttgagtttta ttaaactttt taagtttcta
     1441 ttctatcaaa tatttaaaca atctctgcat ccgaaaagac tacctcgtat agttttacta
     1501 acaatatttt tattagcgtg ttcaaatcct gatttcttgt atcaattatt ttctgttcac
     1561 tttcaataag aaaaaagtct ttacttccat ggataata
//
LOCUS       DAEGIQ010000239         1542 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000239 DAEGIQ010000000
VERSION     DAEGIQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.252"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            32..1498
                     /locus_tag="KMT45_002967"
     CDS             32..1498
                     /locus_tag="KMT45_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBE7006363.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          426 a          290 c          326 g          500 t
ORIGIN      
        1 tctgaaaatt taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt
       61 cgggcagccg aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta
      121 ttacggtatg cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact
      181 tggattacca cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat
      241 gtctagtatc gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt
      301 cttcggtgga gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag
      361 tacattgttt gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt
      421 ttctggcatg gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc
      481 gattttttac atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat
      541 cggtcaagaa tataactatc atttagggtt ctctttagct gctattggga tgttctttgg
      601 attattacaa tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc
      661 gaatcctttg acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt
      721 tgttatcgct gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt
      781 tgtcaataca atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct
      841 gacttcaaaa gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt
      901 ccttgcagca atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt
      961 tgctagtgaa cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca
     1021 atcattgaat ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa
     1081 acttggtgaa cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg
     1141 attatcgtac ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag
     1201 tccattgtgg ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc
     1261 agttggtttg tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc
     1321 tatttggttc ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta
     1381 cacaccggga acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg
     1441 tatcttgcta tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc
     1501 aatgaacata aaaacaggaa ttgcttattt gcagcagttc ct
//
LOCUS       DAEGIQ010000240         1460 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000240 DAEGIQ010000000
VERSION     DAEGIQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.254"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(132..1280)
                     /locus_tag="KMT45_002968"
     CDS             complement(132..1280)
                     /locus_tag="KMT45_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006364.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1297..>1460)
                     /locus_tag="KMT45_002969"
     CDS             complement(1297..>1460)
                     /locus_tag="KMT45_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006365.1"
                     /translation="SASSRLIKRDFPRVKQFLWKEMFWSKSFCLLTTGGAPIDLIKKY
                     IQNQGNNHK"
BASE COUNT          423 a          258 c          233 g          546 t
ORIGIN      
        1 acggttctta acgcttgaag tgggagcttc ttgggaatag agcatacttg atagcgtatt
       61 tctttactaa cagcggtgtc tcttatttac caagctatcc ccgtagttcc tacggttctt
      121 gatgattgga tctaagccaa tcccatagaa tgtagtctta gaccttcggt cagaatattg
      181 atactagcat tgatatcacg atcatgatga gtatgacaaa caggacaagt ccattctcga
      241 atttcaagag atttcttgcc atctttatgt ccgcactctg aacaaatctg actagacgga
      301 aaccatttat ctactttgat gatttttcgt ccataccagt cagccttgta ttgtaattta
      361 gtcacaaact ttgaccaaga aacatcagaa atacttttag ctaatttatg atttcgcaac
      421 ataccttttg tgtttaagtc ttcaatacag ataatatcgt gatttttgat catttctgta
      481 ctcaacttat tcagaaaatc agtacgttga ttcattactt tttcatgcaa tctagctact
      541 ttgcgttttt gtttttgata atttttagct tcaaatagat tggtaccttt atttttagcc
      601 aataaagcac gcctagacaa tttgcgttgt tcacgcttta gtttcttttc cattttggac
      661 gtgaatttat tattatcaat tttttgtcca tcagaaagaa tcgcaaagtc cataatacct
      721 aagtcaatac caattgtgga attagtttta ggtaattcga taatttcttc tttacacaac
      781 aaagaaatat agtatttacc gctagaatga cgtgatattg tagcagattt gataatccct
      841 tttggttgtc tatgaagctt aattcttact aatgacttca atttaggaac tttgatgaat
      901 ttactatcaa tcaaagcaat tgtaccattt tgattattcg ttgtataact ctgaacagga
      961 tttttcttac ttttgaaacg tggaaatcca acggatttat cccgaaaaaa attcttgtat
     1021 gctttatcta aattaagttg ggcattggct aaagcaaggc tatcaatttc tttcaaaaac
     1081 ggaaattctt tcttgtattt agctggtgtc ggaaaagtca tttttttaga agaatcattt
     1141 ttaacttctt catacgcctt ttttcggtca tcaagcatta gattgtagac cttacggaca
     1201 caaccaaaag atttggcaaa gaagatttct tgttcctctg ttggatagat tctgaatttg
     1261 tatgctttta gttgttccat aaagttcacc tactttctat ttatgattgt ttccttgatt
     1321 ttggatatac ttcttaatca gatcaattgg tgcgccacca gttgtcagaa gacaaaaact
     1381 tttagaccag aacatttctt tccaaagaaa ttgtttgact cttggaaagt cacgttttat
     1441 caatcttgag ctggcacttt
//
LOCUS       DAEGIQ010000241         1456 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000241 DAEGIQ010000000
VERSION     DAEGIQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.255"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..400
                     /locus_tag="KMT45_002970"
     CDS             2..400
                     /locus_tag="KMT45_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBE7006366.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="KMT45_002971"
     CDS             384..1079
                     /locus_tag="KMT45_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBE7006367.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          550 a          211 c          293 g          402 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaa
//
LOCUS       DAEGIQ010000242         1453 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000242 DAEGIQ010000000
VERSION     DAEGIQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.256"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            76..837
                     /locus_tag="KMT45_002972"
     CDS             76..837
                     /locus_tag="KMT45_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBE7006368.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1065..1259
                     /locus_tag="KMT45_002973"
     CDS             1065..1259
                     /locus_tag="KMT45_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006369.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          472 a          221 c          273 g          487 t
ORIGIN      
        1 aacaaagaaa atgtgatttt tttcactata ttttatcaca tgaaaattca ttaagaaagt
       61 aggaatcgaa gtattatgca cacaccagaa gaaattatgg aactcagtat tcatcttgga
      121 acaaaaaaaa tacataaacc tatcttagct aaactgattt taggttttat cggaggagcc
      181 atgatttctc taggttattt ggcatacgta cgagtctctg cttctatccc agaatctatg
      241 gctagcttga ctagtttagt tggtgcctcg gtttttccta tcggcctgct ggtcatcctt
      301 cttggcggtg gtgaattgat caccggaaat atgatggctg tggcgattgc ctttatggat
      361 aaaaaagtca cattctcaca gctggttaag aattggtcag tgattacttt tgcaaatatt
      421 attggtgcaa ttttcgttgc ctatttcttt ggacactttg ttggtttaac tcattcaggt
      481 gtctatctcg accaagtgat tcatattgct gatcataaaa tcgctgtttc ttggtggcaa
      541 gcattaggct ccggaatagg ttgtaattgg tttgtcggtt tagcgttatg gttatcatat
      601 ggagcaaaag atgcggccgg caaagtacta acgatttggt ttccagtcat gatttttgtg
      661 gcaatcggtt tccagcatag tgtggccaac tgttttgtga tacctgctgc catatttgaa
      721 ggtcaagcga cttggttaga atttctctcg aattttatcc ccgtttatat tggaaatatc
      781 atcggcggag ctatttttgt ttcaggtttt tattacgtta gttataagca tcattaagat
      841 tgaaggtaat gttcattcga gcattacctt caatctcttt atatactatt tatattttaa
      901 gaacaatagc cttttattat atatattatt caaattagtt atatcgagtg aaaaaaactt
      961 tcaaattgat tctttttatt tatcataaag atatagatac ataatcattt tcacttacat
     1021 atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat tatgtagaag
     1081 taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac gaatatcctt
     1141 tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata aaaataaaga
     1201 acgagctaga aaaagaactg acttgtggca aagctgttgt agatatcatc gaaaagtaaa
     1261 tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc taagattata
     1321 gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt tgtataccat
     1381 atgattattt caatatgtat cctggaattg gagatctcgt agagctatat caagatgaat
     1441 caaatatact tgt
//
LOCUS       DAEGIQ010000243         1451 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000243 DAEGIQ010000000
VERSION     DAEGIQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.257"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>1451)
                     /locus_tag="KMT45_002974"
     rRNA            complement(<1..>1451)
                     /locus_tag="KMT45_002974"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          308 a          429 c          285 g          429 t
ORIGIN      
        1 taagaaaaga agcatttgac tcctcttcag acttactgct tggacagaca tttccgatcg
       61 tctgcattcc ttagcctcct gcgtccctcc attgctcaaa caaaaacaac gggtacagga
      121 atatcaacct gttatccatc gcctacgcct gtcggcctcg gcttaggtcc cgactaaccc
      181 tgggcggacg agccttcccc aggaaacctt agtcattcgg tggacaggat tctcacctgt
      241 ctttcgctac tcataccggc attctcactt ctaagcgctc cagcagtcct cacgatctgc
      301 cttcaacgcc cttagaacgc tctcctacca atacacctaa aggtgtactc cacagcttcg
      361 gtaatatgtt tagccccggt acattttcgg cgcagggtca ctcgactagt gagctattac
      421 gcactcttta aatggtggct gcttctaagc caacatccta gttgtctgtg caaccccaca
      481 tccttttcca cttaacatat attttgggac cttagctggt ggtctgggct gtttcccttt
      541 cgactatgga tcttatcact cacagtctga ctcccggata tgaatgaatg gcattcggag
      601 tttatctgaa ttcggtaacc cgagatgggc ccctagtcca aacagtgctc tacctccatc
      661 attctcaatt ccgaggctag ccctaaagct atttcggaga gaaccagcta tctccaagtt
      721 cgtttggaat ttctccgcta cccacacctc atccccgcac ttttcaacgt acgtgggttc
      781 ggtcctccag tgcgttttac cgcaccttca acctggacat gggtagatca catggtttcg
      841 ggtctacgac tacatactca aacgccctat tcagactcgc tttcgctgcg gctccgtctc
      901 ttcaacttaa cctcgcatgc aatcgtaact cgccggttca ttctacaaaa ggcacgccat
      961 cacccattaa cgggctttga cttgttgtag gcacacggtt tcaggttcta tttcactccc
     1021 cttccggggt gcttttcacc tttccctcac ggtactggtt cactatcggt cactagggag
     1081 tatttagcct tgggagatgg tcctcccgga ttccgacgga atttctcgtg ttccgccgta
     1141 ctcaggatcc tcctaggtgt cttccacatt tcgtctacgg ggtttttacc ctctttgact
     1201 gacttttcca agtcattcga ctatctgaaa gaactaccat attggagtcc tacaacccca
     1261 acaagcaagc ttgctggttt gggcttttcc cgtttcgctc gccgctactc agggaatcga
     1321 attttctttc tcttcctgca ggtacttaga tgtttcagtt ctctgcgtct acctcgaatg
     1381 tgctatgtat tcacacaatc gtaacatcct attaaagatg ttgggttccc ccattcggaa
     1441 atctctggat c
//
LOCUS       DAEGIQ010000244         1443 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000244 DAEGIQ010000000
VERSION     DAEGIQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.258"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(15..1154)
                     /locus_tag="KMT45_002975"
     CDS             complement(15..1154)
                     /locus_tag="KMT45_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBE7006370.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          379 a          310 c          261 g          493 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaa
//
LOCUS       DAEGIQ010000245         1394 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000245 DAEGIQ010000000
VERSION     DAEGIQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.259"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(100..399)
                     /locus_tag="KMT45_002976"
     CDS             complement(100..399)
                     /locus_tag="KMT45_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBE7006371.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            750..1382
                     /locus_tag="KMT45_002977"
     CDS             750..1382
                     /locus_tag="KMT45_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE7006372.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          473 a          223 c          198 g          500 t
ORIGIN      
        1 ttagaataaa aaaatacaga ctctgttcaa ataaatgaac ctagcctgca ttatgagatt
       61 gactatactt tttaattcat tgagatgaat aactcgtttt tataaatgcc caaaagtaat
      121 cggtgccgaa gccattcctc gacctaattc tttttcaaat tttcctattg ttgatatctc
      181 catataaaac ggtgatactg gtttagacaa ttctcccctg attgctaacg gacgtaaact
      241 acgttccaaa ttatttatga ctttaggata atactgacct ttttctaagc ctgtctttgc
      301 gtctatcaaa agtatacgtt ccttttcttt gatctctgga tttaaaacga gatcatataa
      361 ttttagtacc atgttttcta tttcttcact tttagacatc acgatttcct cccaaaaata
      421 tatttcttcc cactacattc tataaacaaa gtatacgcta catctagatt tttttaaatc
      481 attactggat tgttaaaacc tttcaataca ctgttatggc aacgattttt attttaaaaa
      541 cattctaaat taaatcagta aaaattttta ttctaagatt tttattagaa ttacgcctaa
      601 tttgtgtctc tcagggcaat tctaagaacg tttcaagaga tagcgacact atcgttatcg
      661 aaaaaggcga aaacctatct cattttctgc taatgtaagt tggtttagct tttgttaata
      721 tgactttatg aaaatggagg taattaaata tgatttttcc taaagttaat gaagcaagct
      781 tgaataaagg atcctttttt catatctatg aaaacgaaga ttttgaactg tataatatcc
      841 tttcacaaaa agaggtcatc gaaaaccaaa taatctgcca tccaattatt tacaaatcta
      901 ttttcaagta tgcatttttt gcttccaatg attttcaaag aatggagatt accgactgta
      961 tattcgaaaa ctgtgatttt tcaggctgta tattgacaaa aagcatgttg catcgcgtga
     1021 aatttgtcaa ttgtaaatta acaggtacta aattgatgga aagttatatt gggaacacgt
     1081 tattcgagaa ttgtaaaatg gactatgtaa atttatctgg ttcaaacata aaagaaagta
     1141 cctttgagca ctctgtctta tcaagcgcag attttgtaga ctgttccctc actaaaataa
     1201 tgttttctac taatgattta gaaaatgcta gcttttttga tactgattta aaaaatatcg
     1261 atttaagtag caactccttt gaaatgattg aagtacgctc ggaacatcta aaaaattgta
     1321 cgcttaatca gttgcaagca cttggttttg ctagaaaatt tttacaaata aaagtagtat
     1381 aatcaaaatg aata
//
LOCUS       DAEGIQ010000246         1382 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000246 DAEGIQ010000000
VERSION     DAEGIQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.260"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            98..>1382
                     /locus_tag="KMT45_002978"
     CDS             98..>1382
                     /locus_tag="KMT45_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBE7006373.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          468 a          270 c          258 g          386 t
ORIGIN      
        1 cttcagcccc attttcattt taggtattaa aaaacatatc gttctctagt atgattaaat
       61 caccacaaaa taaacaactg agaggacgat acgctatatg aatgattcta tcaaaaaaat
      121 gctgagaata acagagaaag atttgatgat tacagaggtc tcttacgaga cccttcagaa
      181 gaaaaagacg ttggtcgtcg atgctgttct ctcgcctact cctcgtgctt gtagaagttg
      241 tggttctact gtggtagatg gaaacgggaa agcaattata gtgaaaaatg gaaaaaagga
      301 aacgattgtc cgttttgaac aatacaatca tatgcctttg gttatgcgcc taaaaaagca
      361 acgctatacc tgtaaaaact gtcgcaccca ttggacggct caaagttatt ttgtccaacc
      421 cagacattca atcgcaaatc atgttagata taaaattgct tctttactga ctgaaaaagt
      481 atctttatct tttattgcga aaagctgtca ggtatctttg accaccgtta ttcgtacatt
      541 gaaagagttt aaaagctatt taccaaagca atctaagaag atcctcccca gagtattgat
      601 ggttgacgaa tttcgttcgc atgcttccat agaagataag atgagcttta tttgcgcgga
      661 tggcgaaaca ggaaaattaa tagatgtttt gcctacgcgt aaattacctc gattaacaag
      721 ctatttctta ggttgtacca atccagaaga agtagaattc ttggtgacag acatgaacgc
      781 cgcctacttc cagctcacca aacgtgttct gccaaatgcg aaagtcgtga ttgatcggtt
      841 tcatattgtc aaacacatga atcaagcgtt caatgagttg cgtatccgtg aaatgaatga
      901 acttcgtaaa gccggacaga aaagccaggc agaaaaactg aaaaagaact ggcgcttttt
      961 gctaaaaaat cgtgcaaaca tcaaccatta tgaatacaaa acatggaaaa gtttccgagc
     1021 accaaaatac ccatttctta ctgaagcaat gatgattgat cgattgcttg agttttctcc
     1081 gcccctaaag gaggcgtatc ccttttttca tgaattagtt gaagcctttc gagacaaaga
     1141 ccctgactta tttttctcct tattggcaga acttcccgaa acgttagatg acagctttcg
     1201 ggaaaagctt caaaaccttc tgacctatga agaaggcatc accaacgcaa tgatctatcc
     1261 ttattccaat ggaaaaatag aagcgaagaa tacccacata aagacaatga aacgagtatc
     1321 ctacggattt aaatcatttg agaacatgag aattagaatc tttttgatca atcaattaat
     1381 ca
//
LOCUS       DAEGIQ010000247         1378 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000247 DAEGIQ010000000
VERSION     DAEGIQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.261"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            73..1242
                     /locus_tag="KMT45_002979"
     CDS             73..1242
                     /locus_tag="KMT45_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBE7006374.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          503 a          205 c          223 g          447 t
ORIGIN      
        1 ggtagaaaaa aagaaagtcc attctgtaaa attaaagtta ccacaaccga aattttatag
       61 gaggactttc tcatgcccaa ttttactaca gaaattatgg aaacactaat caataaaggt
      121 gatttagatg aattatttcg atgtcattta gaattagctg ttaattcatt attgcaggct
      181 gaattaacag cttttcttga ttatgaaaaa tatgatcgtg ctgggttcaa ttcaggtaat
      241 tcccgtaatg ggaattattc acgttcattc agaacagagt atggagaatt aaatttggta
      301 attcctagag atagaaatgg taagttttct caacaaactt tacctgctta taaaagaacc
      361 aatgactctt tagaaaccac tattattcag ctatttcaaa aaggaattac tatgtctgaa
      421 atctctgaat tgattgaaaa aatgtacggg catcactata cgccacaaac catttcaaat
      481 atgactaaaa tagtttctga agatattatt gcctttaaag aaaggtcctt agaatccaga
      541 tactctgtca tttttatgga tgctactcac attcctttaa aaagacaaac tgtttcaaaa
      601 gaagcagttt atattgtaat tggcattcga ttagatggaa ccaaagaggt tcttgggttt
      661 agtcttgctc caactgaatc tgcttatgtt tggaaagaga ttcttcaaga cctaaaagac
      721 cgtggtttaa aagaggtttt attagtcgta actgatggtt taagtggcat taacgatagt
      781 attcatagtg tttatccaaa tgctcaattt caacaatgtt gtgtccatat atctagaaat
      841 atcgctcata aggttcgtgt tagtgatcga caagaaatct gtagcgattt caaattagtt
      901 tatcaagcaa gttcaaaaga agaagcaaat aatcaaataa ggtttatgat agataaatgg
      961 aaaaagcagt atccacgagt ggttaaattg ctcatgaatc ctgctatatt aaccttttat
     1021 aatttccctc cgtcaatcag aagaactatt tattcaacta atttgattga aggttttaac
     1081 aaacaattaa agaaatatac caaaagaaaa gagcaatttc ctaatgaaga atctctggag
     1141 agatttctag tatctcaatt caacaactat aatcaaaagt ttctatgccg agttcataaa
     1201 ggttttaaag aaatacatga tacattagaa tcaatgtttt aacttgatca acagaatggg
     1261 cttttccatt tacacaaaat tattgacgct cccgacaaaa ttatgatctt gcgtaaagtt
     1321 ataaaatatt aataaaaata ttttattaat gaaaagcttg gtagcacggt tattcata
//
LOCUS       DAEGIQ010000248         1368 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000248 DAEGIQ010000000
VERSION     DAEGIQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.262"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            33..1334
                     /locus_tag="KMT45_002980"
     CDS             33..1334
                     /locus_tag="KMT45_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBE7006375.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          445 a          275 c          252 g          396 t
ORIGIN      
        1 atcaccacaa cacaatcaaa ggagcgttct gtatgcctga tcttcatcat atcaaactac
       61 tacttggaat caaagataaa aacattttca tcacaaatgt ggaatcgaag agcattaaga
      121 atatcaagag tcttgttgtt tctgcgacgt taagtaaaga aattcgtcgc tgtcctttgt
      181 gtaaacagat gaatcatgaa ggcatgatcg ttaagaacgg aaagaaaaaa tcgctcattc
      241 aacttaataa atgtgcgaac caactgactt atcttgcttt agcgaaacaa cgctaccact
      301 gtcgtggctg ccacacctat tttacggcga atacctacat tgtcgataga aactgcttta
      361 tcgctaaaca agtgcgctat aaaattttag aagagctaac tgaaaaacaa gcaatgacga
      421 caattgctaa gcattgcggc gtttcttggt cgaccgtttc tcggacactc gcttctctga
      481 tccccatgac aaaagtaaaa agaaattggt taccgcgttg tttattaatg gatgaatttc
      541 gttcactgaa aaatcaagtc ggtccctact ccttttcttg tatggatggc gatactggaa
      601 aattactgga tattcttcct tcacggaaga aaaaggctct cgtttcttat tttatgcaat
      661 ttgaacgacg cgcacgattg aaggtaaagg tcatcgtgac ggacatgaat gtttcttatg
      721 cctctctgat aaaggaatgt tttccaaagg caaaactcgt tgtggatcgt tttcacattg
      781 tcaaacatct cattcgtaaa tttgaggata ttcgcgtacg aatcatgaaa agctttgatc
      841 gaaacgatcc aacacaagcc aaatattatc gacaattaaa ggcgttgtct cggcttctga
      901 tcaaacgaca ggacaagctt gtttatgaca aatggaccaa atggcgcaat tttggttggg
      961 cgtatctcac cgaaagtgaa gtggtggatc gtctgctttc gatctctgac gaattacgaa
     1021 tcgcctattt ttactatcaa gaaattcttc aagcgtttca cgacaaagag gcggacactt
     1081 tctttaaact tgtaagaacg atgcccaatt ccgttccaaa agaactgcat tctataaaaa
     1141 aagcttttat aaactatgaa tccggcattc gtttagccct cgaattgcct tattcaaatg
     1201 ccaaaataga aaatctgcat acccacatca aagcattgaa acgagtcgct tatggctttc
     1261 gaagctttcg gaaaatgaag accagaattt tcttactaaa taatctgatt acctatgaat
     1321 caaaaaacat ctaaagtaga aaaggaattc taccttagat gttcttat
//
LOCUS       DAEGIQ010000249         1345 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000249 DAEGIQ010000000
VERSION     DAEGIQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.264"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(157..1298)
                     /locus_tag="KMT45_002981"
     CDS             complement(join(157..1070,1070..1298))
                     /locus_tag="KMT45_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE7006376.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          331 a          269 c          284 g          461 t
ORIGIN      
        1 ggacacgatt tgtgagaatt ttttcgagat tccagcgggt gagtatctca tcgtttcgac
       61 cagcggtcaa accaaaaccg tttgaccgct gatcgaagcg atgaagttga aaccctgtgg
      121 aaatctcgcc aaaaaaatgt aagtggtagc taggaactaa gcttgcaatg ccaactcctc
      181 catctgattt ggtgttaaat agttgatact actatggatt cgcttgcgat tgtaaaatcc
      241 ttcaatgtat tgaaataacg ctaaataagc agcttcaaag ttttcatacg cttttgatgg
      301 atagacttct tcttttttga gtgaggcgtg aaacgattcg ataccggcat tatcgtaggg
      361 acagccctta cgactgaagg agtgccgaat gttcaattca ttcagctttg cctcataggc
      421 ttcgctagtg tactgagaac ccaaatctga gtggatgatc aagttctcgg agggtgtttg
      481 gtttaagagc gcattgtcaa gggtttcgat aaccaattct ttcgtcatct gcttgccaaa
      541 cttccaaccg atgactttct ttgtgtggag gtcttgaata gtggagaggt aggtccagcc
      601 atcttttagg gtatgaatat aggtaatatc tgtcacccat ttttgattga tcgacgtagt
      661 ggtaaaatct tggtttaaca ggtttttacg tccttcactt ttaactttcg cttgtttgcc
      721 aggcttgtac ttcttgaggg tgactgaacg caagcctaat tttctcatca gtatttgtac
      781 tttctttaag gacaaggtct cgccacggcc aacaagaatc ttatgaattt taggcgcacc
      841 atagataccc ttgttttcaa agtagattcc tgaaatcaag cggtttaagc gtttgttttc
      901 tgattggcgc ttggaaggtt tctttttctt ccagtcatag tagctggatt tcggtgcttc
      961 caaaacggta cacatttgtt ttacgtcatg atcatgaaca tttgattgaa taaattcgaa
     1021 gacatcactc atttttactt ttgagcgaat atggttaaag ccttttttag gatagtgttt
     1081 tcctcctgca tgcgggccat ttcctttttc atcgctagga tttctgcctc agtcatgccg
     1141 gttgctacat tctttgtatg aagcttcttc caacgataaa tcgtttgtgt agcgatacca
     1201 tattgcgctg ccagttcttc gaccgattgg ccagactggt ttaaatctaa gatcatcttc
     1261 ttaaagtctt catcaaattt tttcttggct tttgccatcg tggacacgtc cttttcttag
     1321 tagttagatt ctaccaaatc gtgtc
//
LOCUS       DAEGIQ010000250         1330 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000250 DAEGIQ010000000
VERSION     DAEGIQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.265"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(18..347)
                     /locus_tag="KMT45_002982"
     CDS             complement(18..347)
                     /locus_tag="KMT45_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE7006377.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(424..696)
                     /locus_tag="KMT45_002983"
     CDS             complement(424..696)
                     /locus_tag="KMT45_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBE7006378.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(696..959)
                     /locus_tag="KMT45_002984"
     CDS             complement(696..959)
                     /locus_tag="KMT45_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBE7006379.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1193..>1330
                     /locus_tag="KMT45_002985"
     CDS             1193..>1330
                     /locus_tag="KMT45_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBE7006380.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          424 a          256 c          172 g          478 t
ORIGIN      
        1 gttttgaagg aacgatttca taagccaatt tctgttgcca actcctcttt agtcattact
       61 ttagaattag taagtgtatc ttcagcaata tctctaaagg aagaatgcat tttgtttttt
      121 ctattcttaa attctacaag aatctcatct ttatcgtagc cttctgcaat tatatcagac
      181 aaaatatctt cgctaaaatc ataaaattct ttttgtggtt caacaaattc ataaagaata
      241 ccattctcta ccaactttgc ttcaacttcg actccattag gaacattaaa atctttaggt
      301 actgtcaaca tgattgaatt gccttgtttt cttgtttttg taatcattta aatccctcat
      361 ttctaacagt atgtaattac ttagtaaata cattatacca cgagataatg tatttacaag
      421 taattactgt gactcgtaat gcgtccttgc tgaataaatt tcaaccacgt gttcatcttc
      481 atctacctta taaacaaccc ggtgttgtct attcaatctt ctagaatacc taccttcatg
      541 tgtaggtttt aacttctcaa atgattgggt agggagatag ggatcttcct ttaaagtctg
      601 aataaccttc tcaaattgag gtttcaaatt agactgtttg attttcttta aatcagcttt
      661 agccgagttt ttaatcatca cagaatagtt attcattata agttatccca atcaatttca
      721 tcaacattag taaatccact atcatctttt tctcgcttac gaacaacacc caaagtgcct
      781 atttgctcaa gtaatagcgt ttctttgatt gctttccaat catccaaacc aataagcacc
      841 gcattatttt cactgcgctc actaatgatt tcaatttccg tatgattctc atttacatct
      901 ttcaacaact tataaaaatt tttccgtgct gagctaggat ttaagactgt ttttgccata
      961 tctactcccc ctctaattta tatctccatt ctaacgtact taattaagta cgtaaattcg
     1021 attgttttgt ttaaattgga gtaccaaaac agcattgtaa agtgttatcc gtttccttta
     1081 tacccgtggt gctagttaat tttttcctag aggaaaatgt ctaaacatgt tggtaaatca
     1141 acaaaaaaac gcatagagta gtctcaaaac tcccaagaaa tgaggactac atatgcgttc
     1201 atcaaaatat actgaacatt atacagatac ttttataaca ttcaaggaaa ttatgagaac
     1261 tgtttctaac atataccaca attgtgtacc agataagatt aaaaaccgaa gaaatactga
     1321 tcaactgaag
//
LOCUS       DAEGIQ010000251         1324 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000251 DAEGIQ010000000
VERSION     DAEGIQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.266"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            133..456
                     /locus_tag="KMT45_002986"
     CDS             133..456
                     /locus_tag="KMT45_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBE7006381.1"
                     /translation="MKKILTIICLGLLIGAAIPVSASADDQTTSRVGITFYQSTNKVV
                     PMKRIVVPDKLDQIAPKEYNQLLPKTNDKQNFFSSLIGISLVLSVALFYYLKKQMVMR
                     RKNEK"
     gene            446..>1324
                     /locus_tag="KMT45_002987"
     CDS             446..>1324
                     /locus_tag="KMT45_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006382.1"
                     /translation="MKNSKSLTLLLLIFSVLLFLTSKKVEAAPRPLTQLGYFHWANQA
                     KTPANNTFIMHHGQQTEVIPLSGNVRGEILTVDRNTNVSSCLVKNVGYYHGEQINLKV
                     TIERNKGNLAGGSIYFSKGYFLGIDIDGEMMVTYEFLNQNNTPVTVETAFNYYGLNPN
                     KYIGFLSPSTLIKGLYANNPTHILYDVWDGGTDDYWVYFKNITRGIPWRDPRQNLELT
                     TNPINKVKFIVHNNDATTSSLGYVTDFLAEPEFPVAYGVDSSFEKANQDIYLRAQQTI
                     PNIEKWEKNKSMELCFN"
BASE COUNT          482 a          179 c          249 g          414 t
ORIGIN      
        1 aataattttt taagattgtg aagtggggaa acgatcttaa aaaatgtgtg aataattcat
       61 atatatataa tagatgattt ggataacttt ctataaaaat gattgtataa aagatgtaga
      121 aaggagacaa aaatgaaaaa aattttaaca attatttgtc tgggattgct gattggggca
      181 gcaatcccag tgtctgcatc agctgatgat caaacaacta gtagagtagg aattactttt
      241 taccagtcta ctaataaggt agtacctatg aaaagaatag ttgtacctga taagctagat
      301 cagattgcgc caaaagaata taaccaattg ttacctaaaa caaatgataa acaaaacttt
      361 tttagcagtc taataggtat aagtttagta ttaagtgttg cgttatttta ctatttaaaa
      421 aaacaaatgg tgatgaggag aaaaaatgaa aaatagtaaa agtttaacat tgttgttact
      481 tatttttagt gtactactat ttttaacatc taaaaaggta gaagctgcgc cacgtccttt
      541 aactcagtta ggatactttc actgggcaaa tcaggctaaa acgccagcta ataatacgtt
      601 tatcatgcat catgggcaac aaactgaggt gataccactc tctgggaacg tccgtggtga
      661 aattctaaca gtggatagaa atactaacgt aagtagttgc ctagttaaaa atgtaggata
      721 ttatcatggt gaacagatta atttaaaagt tactatagaa agaaataagg ggaatttagc
      781 tggggggtct atctacttct cgaaaggtta ttttctaggt attgatattg atggtgaaat
      841 gatggtaacc tatgaatttt taaatcagaa caatacgcca gtaacagtag aaacagcttt
      901 taattattac ggattaaatc ctaataagta tatcggtttt ctatctccaa gtactttgat
      961 caagggattg tatgctaata atcctactca tattttgtac gacgtttggg atggtggaac
     1021 agacgattat tgggtatatt ttaaaaatat aacgagaggg attccgtggc gtgatcctag
     1081 acaaaatcta gaactaacta ccaatcctat taataaagtg aaatttattg tccacaataa
     1141 tgatgccacc acttcatcgt taggttatgt cactgacttc ttagctgaac cagagtttcc
     1201 tgtagcttac ggagtagatt cttcatttga gaaagcaaat caggacattt atttaagagc
     1261 acagcaaacg attcctaata tagaaaaatg ggaaaaaaat aaatcgatgg agttatgttt
     1321 taat
//
LOCUS       DAEGIQ010000252         1294 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000252 DAEGIQ010000000
VERSION     DAEGIQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.267"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            44..>1294
                     /locus_tag="KMT45_002988"
     CDS             44..>1294
                     /locus_tag="KMT45_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBE7006383.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          400 a          258 c          251 g          385 t
ORIGIN      
        1 cctttagaat taatttggct accaattcat aggaggattc aagatgtctc atactcagct
       61 tatcaaagat accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac
      121 taaagaaaaa tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc
      181 agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac
      241 aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa
      301 acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga
      361 aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa
      421 tacttccatg acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt
      481 catgaagcga ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg
      541 ctttgatgaa ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga
      601 cggacaaagc catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg
      661 ctatttttct cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat
      721 gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga
      781 tcgtttccac atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac
      841 cacggtacga cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg
      901 ctattggaaa ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg
      961 tgccagtttt aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg
     1021 tcccaactta cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag
     1081 agatcagtca ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta
     1141 tacaaccact ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta
     1201 cagctattca aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa
     1261 tgcgtacggc tttcgcaact tttacaattt caaa
//
LOCUS       DAEGIQ010000253         1292 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000253 DAEGIQ010000000
VERSION     DAEGIQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.268"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            185..>1292
                     /locus_tag="KMT45_002989"
     CDS             185..>1292
                     /locus_tag="KMT45_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006384.1"
                     /translation="MELLEKAYSSTELIDSYEYEDLKYIKDQRNYSAHPVVTYNGNTW
                     ELRDITKETCQDVIRKSYEIIFLKNPILGKKVTIEIIQYASRIHAMSVTPEEFHSALK
                     NSYLNKLSEKAKENLCKTAFKFVFQLSYGNEEADRDRESTFRLLNALIFDNKEFYLNI
                     LKKEIAKYRFTAESREEINKSLGENQQITYTKGFFLLTFIATHPELQHDFSDETIQNL
                     QISINDFKPKQQVLTEIEYYYRLRSLAVLGNNNEELQNHLNSLVDPILQQKMRVSSLP
                     SSTLETIYNQYLYFGEKEMFIDFMIEHLTDASSFRLADKDMEVLPWIYNKLNQEQFNK
                     LLYNLNGNNQFYKNSNFPPFISNLLDFYNQKFQVN"
BASE COUNT          519 a          189 c          189 g          395 t
ORIGIN      
        1 tagaagtagt ttttatcagg gaaattatcg ttctgcaata gctgtcaatt acagtgcgct
       61 tattttagat ttaattgata aattatctga tttagcaaat atttacgagg ataatacttc
      121 taagaaaatt ttagaagata tagctagact aagaaaaagt gatccaaact ctccaaaatg
      181 ggaaatggaa ctactagaaa aagcttatag ttcaactgag ctaattgata gctatgaata
      241 tgaggattta aaatatatta aggatcaaag aaattattca gctcatcctg tagtcactta
      301 caacggtaat acttgggaat taagagatat aacaaaagaa acttgtcagg atgttatacg
      361 gaagtcctat gaaattatat tcctaaaaaa tccaatatta ggaaaaaaag taacgatcga
      421 aattatacaa tatgcaagtc gcatacatgc tatgtctgtt acacctgaag aatttcactc
      481 tgcactaaaa aatagctatc taaataaact aagcgaaaaa gccaaagaaa acctatgtaa
      541 gacagccttt aagtttgttt ttcaattatc ttatggaaat gaggaagcag atagagatag
      601 ggaaagtact ttcagactac ttaatgccct aatttttgat aataaagaat tctatttaaa
      661 tattctaaaa aaagaaattg caaagtatag gttcacagct gaatcaagag aggaaattaa
      721 taaatcactt ggtgaaaatc aacagatcac gtatacaaaa gggttctttt tacttacttt
      781 tatagctacc catccagaat tacaacatga tttttcagac gagacaattc aaaatttaca
      841 aatatcaata aatgatttta aaccgaaaca acaagtacta acagaaatag aatattacta
      901 ccgtttacgt tcccttgcag ttctaggaaa caacaatgaa gaattacaaa atcacttaaa
      961 tagtctagtt gatcctatac tccagcaaaa aatgagagtt tcttcgctac catcttcaac
     1021 attagaaaca atatataatc aatatttata ttttggtgaa aaagagatgt tcatagattt
     1081 tatgatcgaa catcttaccg atgctagttc attcaggtta gcagataagg atatggaagt
     1141 attaccatgg atttacaata aattaaatca ggagcaattt aacaaattac tatacaacct
     1201 aaatggaaat aatcaatttt acaaaaacag taattttccg ccttttattt ctaatctgtt
     1261 agacttttat aatcagaaat tccaagtgaa tt
//
LOCUS       DAEGIQ010000254         1253 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000254 DAEGIQ010000000
VERSION     DAEGIQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.269"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..697
                     /locus_tag="KMT45_002990"
     CDS             <1..697
                     /locus_tag="KMT45_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBE7006385.1"
                     /translation="KNTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSV
                     KYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMF
                     LRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAI
                     TNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELR
                     EKVLRSIIDKSIE"
BASE COUNT          442 a          189 c          205 g          417 t
ORIGIN      
        1 gaaaaacaca aaagttggcg atgctactat tattcgcttt tgtcaaaaaa ttggtttttc
       61 tggtttttct gatttaaaaa ttgaaattgc aaaggaagac tttagtcaaa aaaaagaaca
      121 tccgactagt gtaaagtact atgatgaaat tgctaatagt ttaacagaag ctctctattc
      181 cactattagg cttttagacg aagaaaaatt gaatgaagcc attaaaatga tcactcaatc
      241 aaaaaacata tatatatttg gagtgggatc tagtggaaat actagtcttg atttagaaaa
      301 tatgtttctt cgcgtaggag ttcaagcaaa agctgtacta gaccctcatt ttcaagccca
      361 agtcgcttct ttattaacag ttaatgattt agtaatcatt ttttctttat ctgggaaaac
      421 gaaagatact tatgattcat tgaaaatagc taaaaaaaat ggcgcaaaaa ttattgctat
      481 aactaactat attcattcac ctataggaaa gtctgcagac ttagttttac aaacagccat
      541 tgaagaattt cttaatggtg gatctttagc tggaaaaata tcacaacttt atatttgtga
      601 tctattagtt catggttatg aacaaaacaa taatattgat tcagttgaat taagagaaaa
      661 agttttgcgc tctattattg ataaaagtat tgagtgaact acccccactt agctaaacct
      721 aacggttttt aacgattgaa gtaggcaatt cttgggatga gagcatactt gatagcttat
      781 tttttactaa tagcggtggc tcttattgac caaatatccc tgtagttcct acggttcttg
      841 attttatcta aagatatttt tatcgtggaa gctattgatc aacttgaaag aaaaacgtgt
      901 agctcattat cactagttta tcgagtatgg atgaagcaat ggatcatcct ctcttaaata
      961 aatttattaa ggatctgatc cttcaaattt tttccatgat cgatgagcag gaacctactg
     1021 aatctaaacg acatcgggca caaggaatta ttaagattgc taagcgaatg ttttctataa
     1081 agggtgttct aagctataca gtgttaattc aaaagattcc caatcatctt gtttataaga
     1141 acaacgttga agattaaaaa agtgtttcaa tgctaaaatt gcaaaatact tcaatgttat
     1201 gagacaaact gtctataaga atcaatttcc acccatttca tcacttgtta aaa
//
LOCUS       DAEGIQ010000255         1246 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000255 DAEGIQ010000000
VERSION     DAEGIQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.270"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            200..742
                     /locus_tag="KMT45_002991"
     CDS             200..742
                     /locus_tag="KMT45_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006386.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="KMT45_002992"
     CDS             898..>1246
                     /locus_tag="KMT45_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBE7006387.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAEGIQ010000256         1235 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000256 DAEGIQ010000000
VERSION     DAEGIQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.271"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>1235
                     /locus_tag="KMT45_002993"
     CDS             <1..>1235
                     /locus_tag="KMT45_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006388.1"
                     /translation="KLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKGYQKRTAEE
                     IKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQFPGATACL
                     GRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKRIESGQIEV
                     IKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANNIGI
                     QIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHARMH
                     KFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDKEAAERGKI
                     MNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWI
                     DKKDGKVMEEDTGRTLHLK"
BASE COUNT          482 a          205 c          270 g          278 t
ORIGIN      
        1 aaattgtgcg aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt
       61 tcgaaagaac aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga
      121 acggcagaag agattaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg
      181 aaaaagcata ctcattctcc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt
      241 ccagaacgat cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct
      301 tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg
      361 aacaagatct ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt
      421 cgagaaatag aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg
      481 atcaaaaaaa cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa
      541 cctgaagact atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa
      601 ggacagtcag aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt
      661 cgagatatga cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat
      721 gttcgtaacg aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactcctacg
      781 cagcatttag ctacaagtat tcatgaatta gcccatgcaa gaatgcacaa attttcagaa
      841 ttagataccg caactaaaga attgcaggca gaaatgactt cttatattgt gtgcaaacat
      901 tttggaatgg acacgttgga gaaagctatt ccttatattg cagcatggac gaagaatggt
      961 cagacgttgg atgataagga agctgcggaa cgtgggaaaa tcatgaatga tgtcagccgt
     1021 gtcgcaaatg aattcattca aaccatttct aatgaaatca atcaatacag agaaagagaa
     1081 ccagagatcc aaccagcaga agtgaagcag gagcataaaa atactgtaga aacggcagaa
     1141 caaaagaata ttgttcctgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
     1201 gaagaagata cgggtcgaac gttacatttg aagga
//
LOCUS       DAEGIQ010000257         1222 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000257 DAEGIQ010000000
VERSION     DAEGIQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.272"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..981
                     /locus_tag="KMT45_002994"
     CDS             <1..981
                     /locus_tag="KMT45_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBE7006389.1"
                     /translation="KDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNK
                     EDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPE
                     GQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKE
                     EVKVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGN
                     FTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSN
                     QDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT          495 a          168 c          252 g          307 t
ORIGIN      
        1 aaagatacgg tgaaagttcc agtagaagtg acagacaatc gttctaatgc agataaatac
       61 aatcctgaag gacaaaaagt gactacagac ttaaataaag agccagatgc atctgaggga
      121 attaagaaca aagaagattt accaaaagga actacttatg cttggaaaga gaaagtagat
      181 gttagcacag ctggaaataa aaaaggtact attgtagtga catatccaga tggatcatct
      241 gatgaagttg aagtagacgt tacagtgaca gatactcgtt ccgatgcaga taaatataat
      301 cctgaaggac aaaaagtgac tacagactta aataaagagc cagatgcatc tgagggaatt
      361 aagaacaaag aagatctacc aaaaggaact acttatactt ggaaagaaaa agtagatgtt
      421 agtacagctg gaaacaaaaa aggtactgta gtagtaacat atccagatgg atctaaagaa
      481 gaagtaaaag ttactatttc tgtagtagat aaaaaagcac cgaataaacc tcaagttgat
      541 cctattacag agggtgacca aattgttact ggtaaaactg aaccaaatgc agaggtgaca
      601 gttacattac cagacggaag tcaacaccat gggacagctg ataaaaatgg taattttaca
      661 gttaaagttc ctaaattaga ggcagggaca aaagtgatag taactgcaac tgatgaatct
      721 ggtaatacta gtgaacctac taatgtagtt gtttcttcca acaaaaaaga cagtggaaaa
      781 aacggtagta aaggtagtaa gactgacaat caaggtagca acagcaatca agacaaaaat
      841 agaggcaaaa gccagtcctc aaaagttttt cctaagacgg gagagagtga cagtaatatc
      901 tttactatta gcggtggttt aatactgtta ggaacgttag gattattagg gtataaaaat
      961 cgaaaaaaag agaatgaata gaaaatcaat ttcttaagtc ttctctgaga agaggaagaa
     1021 tctttttctt cctctttttg cttttttatg atgggaaata agtgttagtt aaagatgaat
     1081 atacttttta tagaaaggaa aagaatgtaa ttaagtggaa aaaatagtaa ttattggttc
     1141 aaaccattca gggctagccg cagctcgatt tctaaactta agcgattcaa attatgaagt
     1201 tattctaatc gataaaaaat ca
//
LOCUS       DAEGIQ010000258         1220 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000258 DAEGIQ010000000
VERSION     DAEGIQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.273"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..805
                     /locus_tag="KMT45_002995"
     CDS             <1..805
                     /locus_tag="KMT45_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBE7006390.1"
                     /translation="LLNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKL
                     QELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKL
                     IAYMQKANADVEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSF
                     GEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEV
                     NKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            877..>1220
                     /locus_tag="KMT45_002996"
     CDS             877..>1220
                     /locus_tag="KMT45_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006391.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          538 a          142 c          197 g          343 t
ORIGIN      
        1 attactcaat aaactaacta tttctaatat agactcgact agggaaaaaa tcataaaaat
       61 atttgataaa attaataatt tattcaagaa tgacaatgct gaattaagga agggtctttt
      121 agagtttaaa cttcaagaac tagaacttca ttactcttat ttagtgaaaa ttaccgaaga
      181 aaaagaacaa caaaaagcaa taaaagaaca gatgatagaa gaggaaaaag ttcgtagaga
      241 aattgatcgt gaaaagaaac gtattgataa agaacaacgt caattcaatt ctgaaatttc
      301 caaattaatc gcatatatgc aaaaagctaa tgcagatgtg gaaaaagaat tatatgcaaa
      361 taaaattcaa gaactggaag aaaaattaaa agaattagaa gttatacgtg aaaatgtatt
      421 acaaagagaa ttaaatacta gagccggtta tgtgtatgta attagtaata ttgggtcttt
      481 tggtgaagac atatataaaa ttggtatgac tagaagatta gaacctatgg atagaataaa
      541 agaattgagt agtgcatcgg ttccatttga atttgacgtt catgcgatga ttttttcaga
      601 ggacgctccg tctttggaaa ctaaattgca taatcatttt agaaaacaag aagttaataa
      661 aattaatcaa agaaaagaat ttttcaaagt ttcactagac gaaatagaga aagttgtttt
      721 agaaaattat aatggaacag taacatttac aaaattagct aaagctgaac aatatagacg
      781 atctttagaa ttgtcaaaag attaaatata taaaaagcct tcgggctttt tcttttaaca
      841 attaatagga acgtatattc gaaaggaata gaaataatgt ggttagaaaa aataaatgac
      901 aaaaaattta agtacaccga acgttatacg gatcccttaa ctgaaaaaaa gagaaaagtg
      961 tccgttactt tatctagcaa ttcacgccaa gcttggaatc aagctaactt acttttgaat
     1021 gaaaaaatcg cagaaaaaat aaaacgaaat gaagaattac cactaacttt tggagaatta
     1081 aaaactaaat gggatgaaaa atacaagcca actgtaaaag aaagttccta tcgaacaact
     1141 caggtttatc tatcattaat ttcaaaatat ataaaagatg atgtacttgt aaaaaacgtt
     1201 aatagtaatc ttatacaaga
//
LOCUS       DAEGIQ010000259         1210 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000259 DAEGIQ010000000
VERSION     DAEGIQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.274"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..238)
                     /locus_tag="KMT45_002997"
     CDS             complement(<1..238)
                     /locus_tag="KMT45_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006392.1"
                     /translation="MSRRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFKERDNLTPEQAELIALRKKNKQLEM"
     gene            complement(915..988)
                     /locus_tag="KMT45_002998"
     tRNA            complement(915..988)
                     /locus_tag="KMT45_002998"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(952..954),aa:Ile,seq:cat)
BASE COUNT          371 a          208 c          216 g          415 t
ORIGIN      
        1 ccatttcgag ttgcttattt ttctttctta gtgcgatcaa ttctgcttgt tctggtgtta
       61 agttgtctct ttctttgaat gagcccgttg attgtgcttg cttcatccat ttatcgaaag
      121 aagaaggcgt aagctcatac tctcgaataa tttctgcgcg aggcttacca gcgagataga
      181 gatcgacgat ttgttgtttg aattcttttg aataggttct tcgttgacgt cttgacatga
      241 aaaatcctcc agtgtgtttt ttattattct acacacctta ttttttctgt ctagtctagt
      301 gtagccgatt catgactgac gtgccggacg cacgtagcga tcatcggcgt tatgactata
      361 taaagtatac tttcgttgat ccagaaaggt ataaagatta aagtaggcta cctaaaaagg
      421 tagcccggaa cgaattttat caccatactt gtgaaaggag atatttttta agttagtatt
      481 aagattgtgt aatatgatga tatctatatt ttatagtatc agtgctataa aatcaaaaat
      541 aagtcactaa ttaactacca ctccaattgt aagccttttt tctcactttt tttcaaaaat
      601 atggtatgct ttttaatggc ttcaaatata aaagagttta aagcgtaaca cacttatggg
      661 gaagtggttt ggggtgcgct ttaaactctt ctttattatt atctcacaat ttaacccaaa
      721 tgtctttcta tttaaaaatc aaagtaaaac ttttcaaata tacagaagta taactatgtg
      781 aaacatcctt tcattaatcc ataaaaggat acataaaaaa gccactcatt tgagtggcaa
      841 tgaagaaaag ctttagcttg tataatactc ttcaaaaaaa ttctaacaca gaacgattca
      901 aatggctacg ttaatgtacc ctgtaggact cgaacctacg accggacggt tatgagccgt
      961 ctgctctgac caactgagct aagggtactg gttgttgcca cataaagcca taaacaatca
     1021 accagtagaa tgtgtggcaa caaacctgtt atcgcatagc ttggagtgtg actatttatg
     1081 ggtgatagtg aagatatgcg ataacatcac tattttatcg aataattttt atagttgtca
     1141 atatagttat gtactgctcc tcaacgagga gctattttta tcgtttagga atatttaaat
     1201 accaacgttt
//
LOCUS       DAEGIQ010000260         1176 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000260 DAEGIQ010000000
VERSION     DAEGIQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.275"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            49..825
                     /locus_tag="KMT45_002999"
     CDS             49..825
                     /locus_tag="KMT45_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7006393.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(923..>1176)
                     /locus_tag="KMT45_003000"
     CDS             complement(923..>1176)
                     /locus_tag="KMT45_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBE7006394.1"
                     /translation="GRDLPESKPNPTIFLKASEQLRIPVEECLVIEDSYNGIKAGKRA
                     NMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
BASE COUNT          424 a          171 c          186 g          395 t
ORIGIN      
        1 tttaataaat tgtgatataa tgaagtataa ctttttaggg agtgtactat gaattttcaa
       61 gataatagac cattatattt acaagtagaa gcttctatac gtaacgatat tcgtgaaaaa
      121 aaatatcttc caggagagca attacctaca gaagaagaat tatgtaaaat ttatggagta
      181 agtaagatta ccattcgaaa agcttttaaa cttttagcag aaagtggaac agttgaacga
      241 ttaaggggga aagggacctt tgtaactcaa aaaaaagaaa gtttaaaatt agctggagtt
      301 catggattta gcgattctct ttctacccga ggacataaaa ttagatatac tgttttacat
      361 tctaaaatta taaaggctga tgagttttta gctaataaat tacaaatcaa tgtaactgat
      421 agagtttaca atattaaacg tttaatgtgg gaagacggtg ccccgatggg aattgataat
      481 tttttcgcat cagaagaaaa atttccaact ttatttgaaa aaggtagcca atatacttca
      541 ttatataaac ttctagaaga tgactatgat gtcaaagcta cgagctctat tatggaaata
      601 aacggtttat ctgcaacacc tgagctttcc gacttattgc aatgtatagt aggagatccg
      661 ctatttattt tagaaaaggt tggcaaagat aaaaataatc aaccatttca ttattctcaa
      721 tccacagttc gttgtgatag aattagttat gttattgaag tcacagaaaa tatcacttta
      781 gatacggtgc ataaaaatga ttcagatcct aaagtaatag attgatatta aaagaaatca
      841 tacccaaagc aatcaatttt gattagattt gggcatgatt tttattttta ctaatattct
      901 ctaaatacat caaaaaactt atttatattc caaatttcac ctgtacttct tgtcgcagat
      961 atttaatatc gtaaataaca tcatctgcta gacttacatc ttgagaaaaa cgtttatctt
     1021 taattgcaag aaccttcata tttgctcttt taccagcttt tataccatta taagaatctt
     1081 ctatcaccag acattcttca actggaattc tgagctgttc tgaagcttta aggaatatag
     1141 taggattagg tttactttct ggcaaatctc ttccgc
//
LOCUS       DAEGIQ010000261         1174 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000261 DAEGIQ010000000
VERSION     DAEGIQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.276"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(58..142)
                     /locus_tag="KMT45_003001"
     tRNA            complement(58..142)
                     /locus_tag="KMT45_003001"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Leu,seq:caa)
     gene            complement(163..233)
                     /locus_tag="KMT45_003002"
     tRNA            complement(163..233)
                     /locus_tag="KMT45_003002"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Cys,seq:gca)
     gene            complement(279..350)
                     /locus_tag="KMT45_003003"
     tRNA            complement(279..350)
                     /locus_tag="KMT45_003003"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Gln,seq:ttg)
     gene            complement(362..434)
                     /locus_tag="KMT45_003004"
     tRNA            complement(362..434)
                     /locus_tag="KMT45_003004"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(399..401),aa:His,seq:gtg)
     gene            complement(437..510)
                     /locus_tag="KMT45_003005"
     tRNA            complement(437..510)
                     /locus_tag="KMT45_003005"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Trp,seq:cca)
     gene            complement(517..597)
                     /locus_tag="KMT45_003006"
     tRNA            complement(517..597)
                     /locus_tag="KMT45_003006"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:Tyr,seq:gta)
     gene            complement(599..674)
                     /locus_tag="KMT45_003007"
     tRNA            complement(599..674)
                     /locus_tag="KMT45_003007"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Phe,seq:gaa)
     gene            complement(710..782)
                     /locus_tag="KMT45_003008"
     tRNA            complement(710..782)
                     /locus_tag="KMT45_003008"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(747..749),aa:Asp,seq:gtc)
     gene            complement(792..864)
                     /locus_tag="KMT45_003009"
     tRNA            complement(792..864)
                     /locus_tag="KMT45_003009"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(829..831),aa:Val,seq:tac)
     gene            complement(880..951)
                     /locus_tag="KMT45_003010"
     tRNA            complement(880..951)
                     /locus_tag="KMT45_003010"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(916..918),aa:Glu,seq:ttc)
     gene            complement(966..1054)
                     /locus_tag="KMT45_003011"
     tRNA            complement(966..1054)
                     /locus_tag="KMT45_003011"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1018..1020),aa:Ser,seq:gga)
     gene            complement(1094..1167)
                     /locus_tag="KMT45_003012"
     tRNA            complement(1094..1167)
                     /locus_tag="KMT45_003012"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1131..1133),aa:Asn,seq:gtt)
BASE COUNT          336 a          306 c          264 g          268 t
ORIGIN      
        1 ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct tagtcaatac
       61 cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag tccagcgcgt
      121 ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta accggatttg
      181 aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc gccattatac
      241 tattatcaat ctacaaaata aagaataaat tatagaaact ggggtagctg gattcgaacc
      301 aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaaaa
      361 gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac
      421 ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac ggttttggag
      481 accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga ttcgaaccac
      541 tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta ctcctccatg
      601 gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg ctctaccaac
      661 tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac ggttccgacg
      721 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
      781 ccagttcatt atggaggtta acgggatcga accgttgacc ctctgcttgt aaggcagatg
      841 ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg ggattcgaac
      901 ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc caggtaaatt
      961 tgctacggag agtaagggat tcgaaccctt gagacagttt gcaccatcta catgatttcc
     1021 aatcatgctc cttcggcctc tcggacaact ctccagagtg aaagaagctc aatactcttt
     1081 ctcaaaatga gaactccggc agtaggactc gaacctacga catcatgatt aacagtcatg
     1141 cgctactacc aactgagcta tgccggaata acat
//
LOCUS       DAEGIQ010000262         1162 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000262 DAEGIQ010000000
VERSION     DAEGIQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.277"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..846)
                     /locus_tag="KMT45_003013"
     CDS             complement(<1..846)
                     /locus_tag="KMT45_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006395.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDF"
BASE COUNT          368 a          207 c          127 g          460 t
ORIGIN      
        1 aaaatcaagt cctctacatt ttaaaggttc acttaaacta ttaatatagt taataatgtt
       61 gtctatcctt ttctctttat cttcaccttc aatctgggga gcatatagtc caatttcctc
      121 ccaataatat gaatctcttt caattcccat gatggtagct ttggctaatt tcaaatcaaa
      181 taacgatttt agaattctta gtgtctcaat aaagtcttct ctcttcaact ctttaatcca
      241 ttgtgaattt tctggattat ctaaaatttc aagttcatct tcattataag tatatttcaa
      301 atttgacttg cttaactcat agataaatga agtttcttga ctcataattc ttttaagtac
      361 ttgactataa gtttcgtttg actttgccca tttcgctaat tctaccactg tattcactaa
      421 taaatcgaaa taatgatgtt ctcgttcaat agtctcagca ttttgaaaat gaaaatcacc
      481 tttataaact ccattctttt ccactgtttt attaatattt tttaaaaacc agaatgagtt
      541 tgcaaattta gtcgtacttt ttcgattaac taagttaata actcttccat gtaataataa
      601 gacaaaaata cataaatttt tagcttcctt attattagat actaattgat caatcatagg
      661 gagccatttt ttgattttca ctaattgatt gctcataaat ctttcactgg acttttgttc
      721 aatcttaaat tgcaaaactt ctaactcata attcccatct tctgatttat ttttgggaac
      781 aactatcaaa cttttataaa gtttatccac atcaaaattt atcttttttg cataatttgt
      841 agccattaat acatctcctt attcataata aatttcatat gacgctcctc tcaaaattta
      901 ccggtagact agaaaaaaga ataaaagata cgtcaattca cactttattc ttaaaaaaaa
      961 actggtctct ctaccggtaa tatcgattga gtccgccttt atcgacttta tccttttttt
     1021 atatctgatt tgtcaaatta agctagacaa tatgtcttta tctatgcagc tcaaaaatac
     1081 ttccaatggt gttcgataat ttaatgattt tcttggaata tggtttcttt tcgaggcaac
     1141 ggattgaata aaggtttcat cc
//
LOCUS       DAEGIQ010000263         1162 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000263 DAEGIQ010000000
VERSION     DAEGIQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.278"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..182
                     /locus_tag="KMT45_003014"
     CDS             <1..182
                     /locus_tag="KMT45_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE7006396.1"
                     /translation="LDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELS
                     GKKLKKVNKMNLEVE"
     gene            182..>1162
                     /locus_tag="KMT45_003015"
     CDS             182..>1162
                     /locus_tag="KMT45_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7006397.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARK"
BASE COUNT          486 a          122 c          205 g          349 t
ORIGIN      
        1 atctagacag tgttactgct ggagaaataa ttaatatatt aaagacatta gctaaagata
       61 gaaataaatg tgtaatagtt gtaacacata gtaaggaagt agcagattct gcggatatca
      121 ttttagaact aagtggtaaa aagttgaaaa aagtaaataa aatgaatttg gaggttgaat
      181 aatgataaaa aatgcatttg cttatgtaac tagaaaaagt ttaaaatcat taattatcat
      241 attagttatt ttatctatgt caactttaag tctcattagt ttatctatta aagatgctac
      301 ggacagagct tcaaaagaaa catttgctaa tataacaaat agtttttcta tggagataaa
      361 tagacaagta aatccaggaa cacctagagg tgggggaaat gtaaaaggtg aagatattaa
      421 aaagatatct caaacagata gtatagactc ctatgtaaaa agaataaaca gtgttgcaga
      481 tttagttgat tatgatatta ttgaaactca agagactctt gcgaatcaat cacctgaaag
      541 agcaaagaat tttaaaagaa cagttatgtt aactggggtt aacgactcaa caaaagaaac
      601 aaaatttgta tcagaagcat ataaattagt agaaggaaag catttagaaa atgaagataa
      661 aaataaaatc ttaatgcata aagatttagc tgaaaaaaat aaccttaaag ttggagataa
      721 gataaaaata aaatctaatt tatttgatgc tgataatgag aaaggtgcag atgaaacagt
      781 agaagtagaa attaaaggtc tattcgatgg gcataatagc ggtgtagtaa gtgcagcaca
      841 agaactatac gaaaatacat taatcactga tattcataca gctgctaaag tttatggtaa
      901 taccgaagat acagctgtat accaagatgc aacattcttt gtaaaaggtg ataagaatct
      961 tgatagtgta ataaaagatc ttgggaaatt agatataaat tggagagaat ataatttgat
     1021 taaaagttca tcaaattacc ctgcattaca acaatctata tcaggtattt actcaatttc
     1081 aaataaatta ttttttggct cattaatatt tgcaggagtt gtagtttcat tattattatt
     1141 cttatggatg aatgctagaa ag
//
LOCUS       DAEGIQ010000264         1137 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000264 DAEGIQ010000000
VERSION     DAEGIQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.279"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            210..479
                     /locus_tag="KMT45_003016"
     CDS             210..479
                     /locus_tag="KMT45_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBE7006398.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            472..729
                     /locus_tag="KMT45_003017"
     CDS             472..729
                     /locus_tag="KMT45_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBE7006399.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          436 a          128 c          204 g          369 t
ORIGIN      
        1 caggattatg tgtattgcgt tagatttatt gattgcccaa gaaaaagcag gatttgaggt
       61 accagcactc aaaaagtgac gtcacttttt atcctaaaaa ctaaaagtga tagcactttt
      121 aattataaga agttagaata ttaatcattt gcttaattgt acaatataat gtacaattgt
      181 tttatagaaa taaataaggg gtgaaaggaa tggaagcagt agcttattca aatttccgcc
      241 aaaatttacg tagttatatg aaacaagtta atgaggatgc tgaaacactt attgtaacaa
      301 gtaaagatgt agaagataca gttgttgtat tatcaaaaag agattatgat tctatgcaag
      361 aaacgttgag aacactttct aataattacg tcatggaaaa aattcgtcga ggagatgaac
      421 aattctccaa aggtgcattt aaaacacatg acttaatcga ggttgaatct gatgattaag
      481 gcttggtctg atgatgcttg ggatgattat ctttattggc atgagcaagg aaacaaaagc
      541 aatataaaaa agattaacaa gttaataaaa gatatcgatc gttccccctt tgctggatta
      601 ggaaaacctg agccattaaa gcatgattta tctggaaaat ggtccagaag aattacagat
      661 gaacatagac tgatatatag agttgaaaat gaaacgatat ttatttattc tgcaaaagat
      721 cactattaac caatcggaag taaggaaagg gtcagaaact taaaagtttt tgatccttat
      781 tttatttacc ctagtcattt aaaaagctaa tatagcttag tgttgattgt tattaatgaa
      841 tgtgtttgtt acgcgtatta cggatataag gttagtaaaa tcatttctaa agttgaggaa
      901 aagtaaatat aaatggctta aatttcaaca atttgaagtt gaatagatat gttataatac
      961 tattgtagtg tgggatgtta gttactaaag gatgacgctt atatatatga ctgaatagaa
     1021 taagcaatag gtttaataat ctattttaaa ttttttgtac tagttttagt taattagcaa
     1081 aaacaacaaa aataaacttc tcatagaatt tagctaaaaa ttaatgattt atttaca
//
LOCUS       DAEGIQ010000265         1106 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000265 DAEGIQ010000000
VERSION     DAEGIQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.280"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>1106
                     /locus_tag="KMT45_003018"
     CDS             <1..>1106
                     /locus_tag="KMT45_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE7006400.1"
                     /translation="SEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATG
                     SKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRK
                     RGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVT
                     TSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATT
                     FFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNL
                     KTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLL
                     SMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT          425 a          133 c          187 g          361 t
ORIGIN      
        1 attctgagat aaaaaagtta tttctagaga cagaagttga caaaattgat attcaaaata
       61 aaaagattag ttgtgtaaaa aaagatggta caagattctc tcaagaatat gacaaagtta
      121 tattagctac aggttcaaag caaattatgc tagatatacc ggggaataat ttagctaata
      181 ttttttcgat aaagaatttt ccaaatgcaa aagagatttt taaagctttg gatgatagta
      241 ctattaaaaa agtaggaatt attggagcag gatatattgg agttgaaatt gctgaagcca
      301 taagaaaaag agggaaagaa gtatatcttt tcgatgttgc agatagagtg ttgtcgactt
      361 attacgaccg tagtttttca gataaagttg aagaaatact atcaaaaaat ggaatacatc
      421 tttgtctatc agaaagtcct aaaaaatatt taggaagaaa aaagattgaa aaaatagtca
      481 ctacaagtgg tacccaatac tctatggata tggtcgtaca agctatagga tttctatcta
      541 atagtccaat tggggaaaag gaactcttga gagatgttag tggagcatat ctaactaatg
      601 aataccagca aacaaatata aaagatatat atgctattgg ggattgtgct acaacttttt
      661 ttacgtccat taatagaagt agtgttgatt tttctatttc taatgctcta agaacagctt
      721 atattgcaac tcaacatatt aacaatcaag attttacacc ttctggttcc caatttacaa
      781 atggcttttc tatatttaat tacaattttt acgcagcagg tttgaattta aaaacagcac
      841 gattattaag tgagtctata gattatatag aaatagaaga tttcattaga cctgcttttt
      901 tgagaaaaaa tagtcgtgta aaattaagaa ttagctttga aaaaaataca aagagaatag
      961 taggtgtaca actttgtagt caagaagatt tatctggact attatcaatg ttttctcttg
     1021 ctatagaaga acaaattaca ttagacaaat tgaaaatgtt agattattta tttcatccta
     1081 gttttagcca accctataat tttatt
//
LOCUS       DAEGIQ010000266         1106 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000266 DAEGIQ010000000
VERSION     DAEGIQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.281"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..377
                     /locus_tag="KMT45_003019"
     CDS             <1..377
                     /locus_tag="KMT45_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7006401.1"
                     /translation="LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYS
                     FARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILF
                     FLYPLSKDKVLENCELLKQKHK"
     gene            387..>1106
                     /locus_tag="KMT45_003020"
     CDS             387..>1106
                     /locus_tag="KMT45_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBE7006402.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFK"
BASE COUNT          400 a          122 c          218 g          366 t
ORIGIN      
        1 ttcttagttc ggttggctat atgggtttgg gtgtgtttaa tactgttatt tgggcaaata
       61 ttactgatgt tattgatgat caagaagtaa agtctgatca aagagaagat gggaccgtat
      121 atgcggtata ttcttttgct agaaaaatag gtcaagctct agctggaggc gtcggaggtt
      181 ggactctttc tattataggc tatgactctt tagcaaatgt acaaacggga gatgttttag
      241 aaaagttata taacgcttca acattaattc catcaatttg ttttctgatt gtaggattaa
      301 ttttattttt cctttatcca ttatctaaag ataaagtctt agaaaattgc gagcttttaa
      361 aacaaaaaca taaataggag agaaatatga ataattggat attttgtatt gattcagatg
      421 gctgtgttat ggatacgatg acttataaac acaatctctt tttcggacca attgctgcag
      481 agatttttga tgttcatcat agagaaaaat tcctacagga ttggaataat attaatttat
      541 attcagagac tcgtggaata aacagatttt taggtttagt taaaacatta gaaaaaaatg
      601 atgtaacatc gattaatcga ttaaagcact gggtatctct aacaaaagaa ttgtctaatc
      661 catctttgaa gagggaaata gaatattctc agtcagagga tttattaaaa gctttaaaat
      721 ggtcggaaga agtcaatcaa cagattagtc aagcaaaagg attagataaa ccctttttaa
      781 gtgctagaga tactataaaa gctctgaaaa aatatgggaa gataattatt gtaagctcag
      841 ctaataagga agctgttcaa gaagaatggg aaagacatga attattatca ttagttgatg
      901 aactgtgttg tcaagataaa ggaaaaaaag aagatattat tcgaagtgta attgaaaatg
      961 gttgtgattt ggataagata ttaatgattg gtgattctcc tggtgatcta gaagctgcaa
     1021 ataaaaataa agttttcttt tatcctattt tggtcaataa agaaaaagaa tcatgggaaa
     1081 acttaagaac tgatgtttta tttaaa
//
LOCUS       DAEGIQ010000267         1100 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000267 DAEGIQ010000000
VERSION     DAEGIQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.282"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            54..260
                     /locus_tag="KMT45_003021"
     CDS             54..260
                     /locus_tag="KMT45_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006403.1"
                     /translation="MKQKVIGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(622..861)
                     /locus_tag="KMT45_003022"
     CDS             complement(622..861)
                     /locus_tag="KMT45_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006404.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          389 a          162 c          198 g          351 t
ORIGIN      
        1 tgaataacga aactatcatt ttaggaaaaa aatatatttg ccaacctatt ggcttgaaac
       61 aaaaagtgat tggagaagta atcagtaaat tagaaaattg tgttgtgatc tgcgtagacg
      121 aatatcatga cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat
      181 atactcacgt ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg
      241 aacatacttt agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc
      301 ttttgaaggc actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag
      361 gttcgcctag agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta
      421 tactgccaga actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg
      481 atccaatcat ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat
      541 cgtaaagcat ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat
      601 ttgctgaaga agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga
      661 cagtttcttt accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg
      721 agaatttttc tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga
      781 aggtatgttc tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact
      841 gataaacaga attcttttca ttatgtacac actccttata gtatcgttta gcatacacct
      901 atgctcagta ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt
      961 cttagtaata catatatgga gagggtgata tcccagttca aaccagatac attggttacc
     1021 atctgtatta acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa
     1081 cgtaggtatg atattatcta
//
LOCUS       DAEGIQ010000268         1099 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000268 DAEGIQ010000000
VERSION     DAEGIQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.283"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(425..784)
                     /locus_tag="KMT45_003023"
                     /pseudo
     CDS             complement(425..784)
                     /locus_tag="KMT45_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          354 a          191 c          202 g          352 t
ORIGIN      
        1 ggatgaaacc tttattcaat ccgttgcctc gaaaagaaac catattccaa gaaaatcatt
       61 aaattatcga acaccattgg aagtattttt gagctgcata gataaagaca tattgtctag
      121 cttaatttga caaatcagat gtctataaaa ttcgttagaa aatacaattt atagatatcc
      181 aattgatttg taaacaaaaa ggagttgtca actaagccac tcctttttgt tgtaattttt
      241 cccaataaga aagtctacta ctttaggaag ataatagtaa gatagaaatg ttgcccccaa
      301 aattattatc cagctggata taattaatca aataaaaagg atcaaaaatg tagtgacccc
      361 caaaagttag acttttttgg agtggagaaa tccactcttt ttttcatgct ctaaaatgat
      421 atttttacac aaccgtaacg gttgttttac gaaattggac gggactttgc cagtttaatt
      481 tctgttttat ccgttcatta ttgtagtaat agatatagct atcgatggct gattttaatt
      541 cctcgaaaca gttataaact ttcccgtgaa aaatctcttg ttttaataag ccaaaaaaat
      601 tctccataag tgaattatct aagcagttcc ctttccgaga catgctttga aagattttgt
      661 actccttcaa tttgtttcta taagctttca tttgatacga ccaaccttga tctgagtgaa
      721 acgtacgacg atagggacaa tcgttagtgg tctggatagc ttcctccaaa ccttccatta
      781 ccgcacgtgt ttctgaatgg tggtagcgac cgatttgaac ggctagggcg gaaatacggt
      841 gcaaatgcac ggtggtttca tgcaatgctt acagggcgta ttttgtaagg gtatgtaacg
      901 gagataatcc cgacggtaat ggatgtgaga ggccaagccg acgctgacgg tagggcaaac
      961 aaacatagtt gcactctagc gatcgttatt tatttttaac ggtcaaacta gggtacaact
     1021 atgcttaaaa cacttcgcct caacttctgg tagcaaaagt gattttgaaa gtcaaaatat
     1081 tttttatgag ttatttata
//
LOCUS       DAEGIQ010000269         1079 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000269 DAEGIQ010000000
VERSION     DAEGIQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.284"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            126..>1079
                     /locus_tag="KMT45_003024"
     CDS             126..>1079
                     /locus_tag="KMT45_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBE7006405.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYK"
BASE COUNT          439 a           97 c          193 g          350 t
ORIGIN      
        1 cgcaaaatag ctattattgc tctgagcttt agagtagatt ggtataggaa attggttgaa
       61 caaaaaaata aaaaaaatta gaatgtttaa attagtaaat aaaagaaaag gaattgaaaa
      121 ctaaaatgtg cagaataagt ataatagttc cagtttataa tgttgaaaaa tatttaaaaa
      181 agtgtgtcga ttcaattcta ggccaaacat ttaccgattt tgaattatta ttaattgatg
      241 atggttctac agataattca ggaagtatat gcgatgaact tgcgaaaaca gacaaccgaa
      301 taaaagtgat acataaggaa aatgggggtc taagtgatgc cagaaatatt ggtattgagg
      361 tagcaaaggg agattttatt gggtttatag atagtgatga ttacattgat gaagatatgt
      421 atgcattttt atataacaat atattgaaat atgatgcaga gttatcaatg tgtggtattt
      481 atgacgtcca taagaataaa gaaattaaaa agttaactcc tttttctcaa ttggttacta
      541 aatctgaagc aattgaatta gtattagatg gtaagctagt tgttgctaat gcagtaagca
      601 agctatataa aaaagaatta tttgagaatg tgagatatcc aaaaggaaaa attgctgaag
      661 acgcagcagt aatactgaaa attatcaatc agtgtaataa aatccatgtt gatacttctc
      721 aaaaatatta ttattatcat cgtggagata gtattactag taaaaaattt acaaaaaaag
      781 attttgatat aattgatgta tggaatgaga atgaaaaatg gattattaaa aattatcctc
      841 aaatgtacga taaagtacat actagagttt gttgggctta ctttgttgtg ttagataaat
      901 tagttttaac tgaagaaaaa ggactgtatc aagaaaaaaa acaagtaaga gagtatttaa
      961 ttgataattt cttttttatt ataaaaaata aacgattaac ttttcaaaga aaaatatcta
     1021 tgttattgct tacttttgga attgctgcgt ataaaatacc ggttaaatat cagtataag
//
LOCUS       DAEGIQ010000270         1078 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000270 DAEGIQ010000000
VERSION     DAEGIQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.285"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            244..>1078
                     /locus_tag="KMT45_003025"
     CDS             244..>1078
                     /locus_tag="KMT45_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBE7006406.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GL"
BASE COUNT          402 a          129 c          219 g          328 t
ORIGIN      
        1 ataaaaaaag cacctgaaaa ggtgtctttt tttgatggtt ttgaacttgt tctttcttat
       61 cttgatacat atagaaataa cgtcattttt attttagttg ctgaaaggtg cgttgaagtg
      121 ttggtatgta tgtgttttaa agtattgaaa acccttaaaa ttggttgcac agaaaaaccc
      181 catctgttaa agttataagt gaccaaacaa ataactaaat agatgggggt ttcttttaat
      241 attatgtgtc ctaatagtag catttattca gatgaaaaat caagggtttt agtggacaag
      301 acaaaaagtg gaaaagtgag accatggaga gaaaagaaaa tcgctaatgt tgattacttt
      361 gaacttctgc atattcttga atttaaaaag gctgaaagag taaaagattg tgctgaaata
      421 ttagagtata aacaaaatcg tgaaacaggc gaaagaaagt tgtatcgagt gtggttttgt
      481 aaatccaggc tttgtccaat gtgcaactgg aggagagcaa tgaaacatgg cattcagtca
      541 caaaaggttg ttgctgaagt tattaaacaa aagccaacag ttcgttggtt gtttctcaca
      601 ttaacagtta aaaatgttta tgatggcgaa gaattaaata agagtttgtc agatatggct
      661 caaggatttc gccgaatgat gcaatataaa aaaattaata aaaatcttgt tggttttatg
      721 cgtgcaacgg aagtgacaat aaataataaa gataattctt ataatcagca catgcatgta
      781 ttggtatgtg tggaaccaac ttattttaag aatacagaaa actacgtgaa tcaaaaacaa
      841 tggattcaat tttggaaaaa ggcaatgaaa ttagactatg atccaaatgt aaaagttcaa
      901 atgattcgac cgaaaaataa atataaatcg gatatacaat cggcaattga cgaaactgca
      961 aaatatcctg taaaggatac ggattttatg accgatgatg aagaaaagaa tttgaaacgt
     1021 ttgtctgatt tggaggaagg tttacaccgt aaaaggttaa tctcctatgg tggtttgt
//
LOCUS       DAEGIQ010000271         1064 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000271 DAEGIQ010000000
VERSION     DAEGIQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.286"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(38..994)
                     /locus_tag="KMT45_003026"
                     /pseudo
     CDS             complement(38..994)
                     /locus_tag="KMT45_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          347 a          178 c          159 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcattcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caac
//
LOCUS       DAEGIQ010000272         1047 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000272 DAEGIQ010000000
VERSION     DAEGIQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.287"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..705
                     /locus_tag="KMT45_003027"
     CDS             <1..705
                     /locus_tag="KMT45_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBE7006407.1"
                     /translation="NLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGII
                     PSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLK
                     VRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRE
                     SFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVS
                     PKQYQLGLVDNFNNIS"
     gene            722..>1047
                     /locus_tag="KMT45_003028"
     CDS             722..>1047
                     /locus_tag="KMT45_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006408.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLD"
BASE COUNT          387 a          115 c          182 g          363 t
ORIGIN      
        1 aatctgttat cacaaaatta cgatccagaa atatatctgt ttctttatga aaataaaata
       61 ctggaatatg ttaaaaatgg agatattcga aatttagaaa acatggtttt taatctgagt
      121 aatggaataa ttccaagtgt tagtggagat acgattcggt ctgaaaaaaa ttattctatc
      181 attgtttttg aaaagttagc acaaacgtct ataacattag gaatggatat aatagaagcg
      241 tatcaaagta gagatgcttt gatacaagaa aacgaattag ctgtatcgtt acctgaggtc
      301 ttaaaagtga gggattctgg gattgtttat tatactaagg aaatcggaaa aactaagata
      361 gagcatcttt ctcccttaat ttcttctgtc gttcaattca tcggcttaaa tatctataaa
      421 agaattactg taaaggaaat agctaattat ttttctgtaa gtgaaactaa attaagagaa
      481 agctttaaga atgaaatgca tattacgatt tataattata tctctaagcg aaaaatttct
      541 acggctaaaa taatgttgaa atcaaaccat actatcagtg aagtttcttt aggtttagga
      601 ttttcagatt cttcacactt ttcgagagta ttcaaaaagt acgcaggtgt atctcctaag
      661 caatatcaat taggtttagt ggacaatttc aacaacatta gctgagaggt tttattttat
      721 tatgagacta tatcatttac gtagtttgga ggagttgagg aaattggatt ttaacaaaaa
      781 atttttttgg gaaatttctg atttgagttg tttcgtagaa tctgagcgtc taagtaatgg
      841 taataatagt gtgttaaatg aatatgacaa agtagtaatt aataaagtaa aaagtttatg
      901 tgaagaagct agagaatcta tcagtcatat taatggtagc aattttaaac aaacattatt
      961 taatctgatt aagctagatg ctaaaatatc aagttattta ttctttttga tacatgatga
     1021 atttatggat tatcaaaaat tggatca
//
LOCUS       DAEGIQ010000273         1041 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000273 DAEGIQ010000000
VERSION     DAEGIQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.288"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            76..1035
                     /locus_tag="KMT45_003029"
     CDS             76..1035
                     /locus_tag="KMT45_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBE7006409.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          376 a          189 c          183 g          293 t
ORIGIN      
        1 agaaaaataa aaaggcattt atggtacact caaattgtat ttccgacgaa agaaaacaaa
       61 ggagtgtaac cataaatgac ctacaaacat cttaccatag acgaactgac aatgatagaa
      121 tcatattatc ttcaacataa taagccggtt gaaatcgcta accgaatggg acgtgctata
      181 caaactattt ataatgtagt caataagttc aagcaaggca agactgctct tgattattgg
      241 caccagtata aagaaaataa gaaaaaatgt ggtagaaaag tcattcaatt acctgctcat
      301 gaagtagatt acattaaaga gaaagtcact cttggttgga cgcctgacgt cattatcggg
      361 cgaaaagaaa ggcctgtttc atgcggtatg agaacacttt atcgtttatt ttctaaagga
      421 atatttgata ttgacacact accgatgaaa ggtaaaagaa aacccaatgg ccatcaggaa
      481 aaacggggaa aacaacagta tcagcgctca atccatgatc gacctgataa ttatcctgat
      541 ttcaattctg agtttggtca ccttgaaggt gatacgatcg ttggcattca tcataaaagt
      601 gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa acccaacggc
      661 cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt ccctaaaaac
      721 ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt
      781 aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca acgcccatta
      841 aacgagaatt ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat ggattttaga
      901 aaagtgaatc agacatttat ttccagtgtc agcaatcaac gtaatcatat tccaagaaaa
      961 tcattgaatt acagaacacc aatcgaaata tttttgagct atgtacaaga agcattttat
     1021 tctagcttaa tttgacaaat c
//
LOCUS       DAEGIQ010000274         1033 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000274 DAEGIQ010000000
VERSION     DAEGIQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.289"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(61..279)
                     /locus_tag="KMT45_003030"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="KMT45_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="KMT45_003031"
     CDS             complement(545..739)
                     /locus_tag="KMT45_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006410.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="KMT45_003032"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="KMT45_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          357 a          178 c          153 g          345 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gta
//
LOCUS       DAEGIQ010000275         1027 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000275 DAEGIQ010000000
VERSION     DAEGIQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.290"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            46..612
                     /locus_tag="KMT45_003033"
                     /pseudo
     CDS             46..612
                     /locus_tag="KMT45_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          354 a          176 c          217 g          280 t
ORIGIN      
        1 ttctacctta tttgatagta gatttaaaac tttgcaacag aacccgaaga acaattgtat
       61 ttggaaagtg aaacgagtca aacgaaggtc acagtcagcc ataatcgttt agacgtgtat
      121 aaacgtcgtc ctgaaacggt aaaaaaagga acggagcttc tacgactaga aacagaaaaa
      181 tacatcatga atgtttacct agaaaggagt gagctggtcg aaaactttgt cagctacacg
      241 atttgcagga aacactatta ctcagaatca gaatattatt caatttttga gagcatggaa
      301 ttaatggaag aagaaatgga agagctaaac agcaaagatg cgacgataga cgattattac
      361 cttgtattta ctagattttt gacgtgtgga acggaatcta tcgtgttaat gggaatggat
      421 tatgaactcg ctgtacccga agcatttttg agggaatatg tagaagaagt agataaccca
      481 ggcgacgact ggtacacgac tttaactgat tttttagaca cctatactta cgacgataca
      541 gaagaaatcc aaaatgaact tgaccgtaaa cgaatgccat atcaacgatt tgaaaggaaa
      601 aaaaggcaat aatcatttat accgaaaagt gagaatgtgt gaaataagga caaaaaacct
      661 agtaactaag ggaatggttc ttatttcgca ccaatcgctc caaccgcccc cgccgcttag
      721 gtggtgcgaa ataagcgggc aacacccgat aacctaagag aaagaaggag atccatatga
      781 ataaaatcat tcctctattg ttagtcctat ttgttggcga aatacttatg cgccatgctg
      841 cagctgtgca tttaaacttc gtagtatttt tggcaatact ggtattttgt ttgatctttt
      901 ggcgctttgt attagccctt gtgagtctac tattcaagat gattgcagcc atatttttca
      961 tagggttatt tcgatgaaag gagagagtac taatgccaat tgcaatagga aataaacggt
     1021 tacccgt
//
LOCUS       DAEGIQ010000276          995 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000276 DAEGIQ010000000
VERSION     DAEGIQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.291"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(69..>995)
                     /locus_tag="KMT45_003034"
     CDS             complement(69..>995)
                     /locus_tag="KMT45_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBE7006411.1"
                     /translation="YESLLVKICEDYYLEDINISEISKKYGITRYKALKYIEDAREKQ
                     LVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADYIQELIKK
                     ANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQKAAERYES
                     SYLTLSIPLYVINETARELMALEPSLANTLATASRADMLISGIGTLSSIDSVEPWNKH
                     RKILFPSMTDTVGFLYGRPFTINGEFIKPSEDKTFGLSLKEIFDIPKRIGICKSKFKA
                     ESCLGALNGDFFTDLFLDEKTAWKILSLIKKE"
BASE COUNT          335 a          172 c          157 g          331 t
ORIGIN      
        1 caataaaaaa gagtgaatca ttgtattgtc ccccaaaaaa tctaactttt tgggggattt
       61 tacatgactc actctttttt tatcaaagaa agtattttcc aagcagtttt ttcatccaag
      121 aataaatcag taaaaaaatc accattcaaa gcacctaaac aactttccgc tttgaactta
      181 cttttacaaa taccaattcg ttttggaata tcgaaaattt cttttaaaga aagaccaaaa
      241 gtcttatcct cggatggttt tatgaattca ccgttgatag tgaaaggtct tccgtaaaga
      301 aatccaacag tatccgtcat gcttggaaat aggattttac gatgcttgtt ccaaggttcg
      361 acagaatcaa tagaggacaa ggtgccaatt ccagaaatca acatatctgc acgcgaagcg
      421 gttgctaagg tattagctaa agaaggttct agagccatta gttcacgtgc agtttcattg
      481 atgacatata aaggaatgct gagagtcaag taagaagact cataacgttc agcagctttt
      541 tgaactaatc gtgtggagcc agctaaggaa ttatattttc cgatttcgcc aatgaattga
      601 gtaaaaatta attcttcacg agcacccatt ttaaactgat caatcacttt gtaaacggta
      661 tctccccaag atagtgcaac cacattggct ttcttaatta actcttgaat gtaatcggca
      721 gcaaattttg aaaataataa atcgtgttgt gataagtctt cactactttt taatatatag
      781 atattcgttt taaagtaatt tttgaagatt tcttctagct cataattcct agcatacgga
      841 gaattaattg aaatactaac taattgtttt tcacgtgcat cttcaatata ttttaaagct
      901 ttatatctgg tgatcccata ttttttagaa atttcactaa tattgatatc ctcgagataa
      961 tagtcttcac aaatttttac aaggagtgat tcata
//
LOCUS       DAEGIQ010000277          994 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000277 DAEGIQ010000000
VERSION     DAEGIQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.292"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>994)
                     /locus_tag="KMT45_003035"
     rRNA            complement(<1..>994)
                     /locus_tag="KMT45_003035"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAEGIQ010000278          991 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000278 DAEGIQ010000000
VERSION     DAEGIQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.293"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(21..94)
                     /locus_tag="KMT45_003036"
     tRNA            complement(21..94)
                     /locus_tag="KMT45_003036"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="KMT45_003037"
     tRNA            complement(108..181)
                     /locus_tag="KMT45_003037"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="KMT45_003038"
     tRNA            complement(209..281)
                     /locus_tag="KMT45_003038"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="KMT45_003039"
     tRNA            complement(301..374)
                     /locus_tag="KMT45_003039"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="KMT45_003040"
     tRNA            complement(384..457)
                     /locus_tag="KMT45_003040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="KMT45_003041"
     tRNA            complement(468..553)
                     /locus_tag="KMT45_003041"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="KMT45_003042"
     tRNA            complement(570..641)
                     /locus_tag="KMT45_003042"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="KMT45_003043"
     tRNA            complement(653..725)
                     /locus_tag="KMT45_003043"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="KMT45_003044"
     tRNA            complement(743..824)
                     /locus_tag="KMT45_003044"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="KMT45_003045"
     tRNA            complement(832..904)
                     /locus_tag="KMT45_003045"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="KMT45_003046"
     tRNA            complement(908..980)
                     /locus_tag="KMT45_003046"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DAEGIQ010000279          975 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000279 DAEGIQ010000000
VERSION     DAEGIQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.294"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..806
                     /locus_tag="KMT45_003047"
     CDS             <1..806
                     /locus_tag="KMT45_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBE7006412.1"
                     /translation="LLGSPKNLKFFESPIDLLSYAALNRETIKDTWLVSMDGLKDAVV
                     GHYVREAVTELAPKEEFPESIEICVDNDRAGHVFWDKVHRLGVSNPFTGKAVYFEPNL
                     PNDWQVPREYQTIYEEVGKEMRVAPEAIMAIHKIENNLTDTNQIVSFGEIQGVFAKKL
                     APKEKAQSIVVKEKCIEAAQQLKSCEKSDGTYDFDRFYRGKGKINEQIQFSLKAKHYF
                     EGYKNHEHEFVSEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            817..>975
                     /locus_tag="KMT45_003048"
     CDS             817..>975
                     /locus_tag="KMT45_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HBE7006413.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVEL"
BASE COUNT          339 a          158 c          224 g          254 t
ORIGIN      
        1 ttcttcttgg atctcctaag aatctgaaat tttttgagag tcccattgat ttacttagtt
       61 acgctgcttt aaatcgcgag acgatcaaag acacgtggct tgtttctatg gatggactga
      121 aggatgcagt agtggggcac tatgttcgag aagcggtgac agaattggcg ccgaaagaag
      181 aatttccaga atcgattgag atttgtgtgg ataatgatcg agctggacac gtattctggg
      241 ataaggtaca ccgacttggt gtgtcgaatc catttacagg aaaagcggtg tattttgaac
      301 cgaatcttcc caacgattgg caagtaccga gagaatatca aacaatctat gaagaagtag
      361 ggaaagaaat gagagttgcg cctgaagcaa tcatggctat tcataaaatc gaaaacaacc
      421 taacggatac taaccaaatc gtatcgttcg gtgaaatcca gggagtattt gccaaaaaac
      481 ttgcgccaaa agagaaagcc caatctattg ttgtaaagga aaaatgtata gaagcagctc
      541 agcagctaaa atcgtgtgaa aaatctgatg gaacatacga ttttgatcgt ttttatcgtg
      601 gaaaagggaa aattaatgaa caaattcaat tttcattgaa ggccaaacac tattttgaag
      661 gttataaaaa tcatgaacac gaatttgttt ctgaagtgaa aaaagattgg aatgatcaat
      721 tgaagcatga gatccagcaa caagaaatca gaaagcaaaa acgtgcgatg ttgtttcaac
      781 aaggcagaca acaagaaagg gagtgaagtc gtcactatga aaaccgtcat tttagcagaa
      841 aaaccgtcac aagcgaaggc ttatgccgat agcttttcta aagctacccg aaaagatggg
      901 tattttgaga tccaagatcg attgtttaca ggagaaactg tgatcacgta tggttttggt
      961 catttagtcg agtta
//
LOCUS       DAEGIQ010000280          952 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000280 DAEGIQ010000000
VERSION     DAEGIQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.295"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>952
                     /locus_tag="KMT45_003049"
     CDS             <1..>952
                     /locus_tag="KMT45_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBE7006414.1"
                     /translation="LEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADTIVIATDSDR
                     EGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYPKFEEVQARQ
                     IADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEG
                     KAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVETEKKSAASP
                     RLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHL
                     DEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTV"
BASE COUNT          336 a          193 c          193 g          230 t
ORIGIN      
        1 cattagaaca tttgcctatt ttccccaatc aataccacta tcatgttcct aaagataaga
       61 aaaagcagtt tagtgtcgtc aaacgacagc tgcagtcagc tgatacgatt gtgatcgcga
      121 ctgatagcga tcgtgaaggg gagctgattg cctggtcaat cattcaacaa gcaggcgcca
      181 atcaaggaaa aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc
      241 agggattcca acagttacga gacgcagaag agacctatcc aaaatttgaa gaagtacaag
      301 cacgtcagat cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct
      361 tgcagcaaaa aggcatacca ggaagttttt ctttaggtag agtccaaacc ccaacactct
      421 atatgattta tcaattgcag gaaaagatta gaaacttcca aaaagaacca tattttgaag
      481 gaaaagccca agtaatcgct caaaatggcg catttgatgc gaagcttgat ccaaatgaaa
      541 cccaagcgac acaagaagca tttgaagcct atttaaaaga aaaaggtgtt caattaggaa
      601 aacagccagg tacgatcctt caagtcgaaa cagagaaaaa aagtgcggct agcccacgtc
      661 ttttttcttt atcgagcctt cagtctaaaa tgaatcaact catgaaagct agtgccaaag
      721 atactttgga agccatgcaa gggttgtatg aaggaaaata tctaagctat ccgcgaacgg
      781 atacccctta tattacggaa ggggaatatg cgtatctgtt ggatcactta gacgaataca
      841 agcatttttt aaaagccgaa gcgattccga ctcctattca tacaccgaat agtcggtatg
      901 tgaataataa gaaagtccag gaacattacg cgatcattcc tacaaaaacg gt
//
LOCUS       DAEGIQ010000281          943 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000281 DAEGIQ010000000
VERSION     DAEGIQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.296"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            6..>943
                     /gene="bsh"
                     /locus_tag="KMT45_003050"
     CDS             6..>943
                     /gene="bsh"
                     /locus_tag="KMT45_003050"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBE7006415.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYP"
BASE COUNT          325 a          125 c          170 g          323 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tcc
//
LOCUS       DAEGIQ010000282          921 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000282 DAEGIQ010000000
VERSION     DAEGIQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.297"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            11..>921
                     /locus_tag="KMT45_003051"
     CDS             11..>921
                     /locus_tag="KMT45_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006416.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSN"
BASE COUNT          387 a          138 c          214 g          182 t
ORIGIN      
        1 ggaaacggac atggcagaag aaaaagaacc actagacaat agtcgcttgg gaaaatccaa
       61 aagaaaatta gtacgattgc aaaatgaatt aaacgaacaa attgagaaga tgtttgaaca
      121 ccagagaaag accaacggtc agccaatgaa cgataagaga aatgggcatt cttggtttag
      181 gcaacaagaa cgtttagaaa acaaagtgca tagtctgaga gaagagatca agcaacaaga
      241 aaaacaggta gaaaaacttg aaagacaaga agagctaaag gaaatgggct ataacaagta
      301 cggcggactg gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt
      361 tgaaaaaggt gaatcgactt tttcagccgc cacgatccga aaatatcaac ggaagttgga
      421 aacattagaa cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt
      481 acaagcgatc attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt
      541 tttgaaaggc tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc
      601 aaaaaaatac caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga
      661 ggctgcacca actgattctc tgaaggagaa agaaaaagat catttctatc gagtagaatt
      721 caacgaaaat gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattaga
      781 attgatcgat caacttaaaa cgttggataa ccaatacgct gcagaagcag gttattataa
      841 attctatttt gaagaagtga agaacggaga agtagtagat cagatccgaa tggatctagg
      901 ggatggcgtc aacagtaatc a
//
LOCUS       DAEGIQ010000283          911 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000283 DAEGIQ010000000
VERSION     DAEGIQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.298"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..535
                     /locus_tag="KMT45_003052"
     CDS             <1..535
                     /locus_tag="KMT45_003052"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006417.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKIRPFIF"
     gene            562..>911
                     /locus_tag="KMT45_003053"
     CDS             562..>911
                     /locus_tag="KMT45_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006418.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSN"
BASE COUNT          381 a          138 c          209 g          183 t
ORIGIN      
        1 catggcagaa gaaaaagaac cactagacaa tagtcgtttg ggaaaatcca aaagaaaatt
       61 agtacgattg caaaatgaat taaacgaaca aattgaaaag atgtttgaac accagagaaa
      121 gaccaacggt caaccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      181 gcgaatcgaa aataaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaagt
      241 agaaaaactt gaaagacaag aagagataaa ggaaatgggc tataacaagt acggcggact
      301 ggatatgacg atcgagaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      361 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      421 acagctgaaa gaaagatcgg aaaaaggaaa agaaaacctt cttcctgaag tacaagcaat
      481 cattgacagc ggacgagtga cgcaatggaa aaaaatccga ccatttattt tctgaaaggc
      541 tatcgaaaag tcgctttaga actgaatgaa aacggggaat ttgaggaatc taaaaaatac
      601 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgtacca
      661 actgattttt tgaaggagaa agaaaacgat catttttatc gagtagaatt caacgaaaat
      721 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
      781 caactcaaaa cgttggataa ccaatacgcc gcagaagcag gttattataa attctatttt
      841 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
      901 aacagtaatc a
//
LOCUS       DAEGIQ010000284          900 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000284 DAEGIQ010000000
VERSION     DAEGIQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.299"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            73..234
                     /locus_tag="KMT45_003054"
     CDS             73..234
                     /locus_tag="KMT45_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBE7006419.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>900
                     /locus_tag="KMT45_003055"
     CDS             253..>900
                     /locus_tag="KMT45_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE7006420.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFV"
BASE COUNT          380 a           79 c          154 g          287 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
//
LOCUS       DAEGIQ010000285          891 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000285 DAEGIQ010000000
VERSION     DAEGIQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.300"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            1..865
                     /locus_tag="KMT45_003056"
                     /pseudo
     CDS             1..865
                     /locus_tag="KMT45_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
BASE COUNT          331 a          160 c          211 g          189 t
ORIGIN      
        1 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt aaaggcaatc
       61 gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag cgacggaaaa
      121 gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt gacggtggat
      181 ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg tacgttgatc
      241 actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc catattgaaa
      301 gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac cttgaaacaa
      361 aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg gattttgctt
      421 tgccaagccg tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc caaatgggaa
      481 agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct agccaatatc
      541 caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc cactgatttt
      601 ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta
      661 ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt ggcagcttgt
      721 acgtctgaag cctgtgattt taaaaggtca gaagggttca tttgaagcaa cgattgtttt
      781 aaaagaagac ttcactacag gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa
      841 aagaacacgc cgcactgcaa aataatggag ggttaatctt atgcgtaata c
//
LOCUS       DAEGIQ010000286          883 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000286 DAEGIQ010000000
VERSION     DAEGIQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.301"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>883)
                     /locus_tag="KMT45_003057"
     CDS             complement(<1..>883)
                     /locus_tag="KMT45_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7006421.1"
                     /translation="YKASYNYHLIDFMYKYPRSVLKDFAKEQFTSVSTVFRYAKLLIP
                     YFRRYHITFHPFQLELNTSEANIRSFFYYFYWNSTRESSDKWPFHIEQKEIEKYIVAF
                     EGIYDITLTIFQKRVFSFWLAINIERSSFRKVRVDNEYKSVISDDPHFNLLKKWSKQI
                     NLSFNSDELCFLYRIIYSFGVIDGNAIYENSHAYAHQRQNTCSYRAVENLEKVLQSMF
                     RFSLDIKDPELIFNFIAFHERSYLFYGNPDLFFNRSYIEEMKEEEPRTYHIMEKLKKE
                     LQANADLDVSKKLENWA"
BASE COUNT          314 a          144 c          115 g          310 t
ORIGIN      
        1 tgtgcccagt tttctaattt ctttgatacg tccaaatccg catttgcttg taattctttt
       61 ttgagttttt ccattatgtg ataagttcga ggttcttctt ctttcatttc ttcgatatac
      121 gaacgattaa aaaaaagatc tggattacca taaaatagat aacttctttc atgaaatgca
      181 ataaaattga atatgagttc tggatccttt atatctaaag aaaaacggaa catgctttgt
      241 aaaactttct ccaaattctc aactgctcga taagaacaag tattttgtcg ctgatgtgca
      301 tatgcatgac tattttcata tattgcattt ccatcaatta ctccaaaaga ataaattatt
      361 cgataaagga aacacagttc atcgctgtta aaagaaagat tgatttgttt tgaccacttc
      421 ttcaaaagat taaaatgcgg atcatctgag ataactgatt tatattcatt atctactcgt
      481 acttttctaa atgaacttct ttctatatta atggctaacc aaaaagaaaa tacacgtttt
      541 tgaaaaatag ttaacgtaat atcataaatc ccctcaaaag caactatata cttctctatt
      601 tccttttgtt cgatatgaaa tggccactta tcactacttt cacgtgtaga attccaatag
      661 aaataataaa aaaagctacg aatgtttgcc tctgatgtat tcaattctaa ctgaaatgga
      721 tgaaatgtta tatgataacg cctaaaatat ggaattaaga gcttcgcata tctgaaaaca
      781 gtagatacac tagtaaattg ctctttagca aaatctttta gaacacttct aggatattta
      841 tacataaaat caatcaaatg ataattataa gaagctttat aaa
//
LOCUS       DAEGIQ010000287          871 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000287 DAEGIQ010000000
VERSION     DAEGIQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.302"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="KMT45_003058"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="KMT45_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBE7006422.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          251 a          185 c          133 g          302 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa c
//
LOCUS       DAEGIQ010000288          864 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000288 DAEGIQ010000000
VERSION     DAEGIQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.303"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..675
                     /locus_tag="KMT45_003059"
     rRNA            <1..675
                     /locus_tag="KMT45_003059"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            770..>864
                     /gene="rrf"
                     /locus_tag="KMT45_003060"
     rRNA            770..>864
                     /gene="rrf"
                     /locus_tag="KMT45_003060"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          237 a          180 c          257 g          190 t
ORIGIN      
        1 cagtgtcaga tgggcagttt gactggggcg gtcgcctcct aaaaggtaac ggaggcgccc
       61 aaaggttccc tcagaatggt tggaaatcat tcgaagagtg taaaggcaga agggagcttg
      121 actgcgagac caacaagtcg agcagggacg aaagtcgggc ttagtgatcc ggtggttccg
      181 catggaaggg ccatcgctca acggataaaa gctaccctgg ggataacagg cttatctccc
      241 ccaagagtcc acatcgacgg ggaggtttgg cacctcgatg tcggctcgtc gcatcctggg
      301 gctgtagtcg gtcccaaggg ttgggctgtt cgcccattaa agcggcacgc gagctgggtt
      361 cagaacgtcg tgagacagtt cggtccctat ccgtcgcggg cgttggaaat ttgagaggag
      421 ctgtccttag tacgagagga ccgggatgga cttaccgctg gtgtaccagt tgttctgcca
      481 agggcattgc tgggtagcta tgtagggaag ggataaacgc tgaaagcatc taagtgtgaa
      541 gcccacctca agatgagatt tcccatttct ttaagaaagt aagatccctg agagatgatc
      601 aggtagatag gtcaggagtg gaagtacagt gatgtatgga gcggactgat actaatcgat
      661 cgaggactta accaataggt taaaagaaaa gaactcggaa aaaaacttat gtgacttcaa
      721 atccagtttt gagtgaacaa aatttactca ataaaagata acaccacagt gtggtggcga
      781 tagcgagaag gatacacctg taaccatgcc gaacacagaa gttaagcttc ttagcgccga
      841 ttgtagtgag gggttgcccc ttgt
//
LOCUS       DAEGIQ010000289          852 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000289 DAEGIQ010000000
VERSION     DAEGIQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.304"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..832)
                     /locus_tag="KMT45_003061"
     CDS             complement(<1..832)
                     /locus_tag="KMT45_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006423.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     I"
BASE COUNT          348 a          129 c          136 g          239 t
ORIGIN      
        1 aaataaaagc accaatcaat ccgagaaata agaataatcc aatagtatag ttgttatagt
       61 tattatttaa aatatgtgtc agcatatcac taggagccat accacttaat tcttttccca
      121 gtgggacttt caattcttgt gctctagatt gttgaataaa aggaattaag aatcccgcac
      181 ttaaaaaaaa ggtgaggacg gtagctttta aaagacttaa gagtctagcg attttttggt
      241 cccaaaaata aaaagaaaga aacaacgttc ctccaataaa tacagaaacc atagcgacag
      301 aaagtaaatg agtatacact actagcgtca tgccaataga caaccagtac catttttggt
      361 aatctctaat ataaatttcg taacacccca ttagtatcaa aggcaaaaat gttaaagcga
      421 cagcttctcc caatgaagca cgatggaata tgtcaatggc acgataggcg gaaaatgtat
      481 aaataatcga aaataacagc gaaatataac gattgttttt tatttgtttc atagaaaaat
      541 aagaaacgac cattgttaaa aaagtgataa aaaaatagta gatattataa cttaaaacaa
      601 gatttcccat cattttataa aataaaaaag ctggataaag agtaagccat ggataaaaaa
      661 tattcatcat tgttccatga tgatcgaagt tattgaagtt tactggagaa ctccatacat
      721 tatctagtcc tatgattcga gataaatgaa agtaagtatc ctgattgaca ctatgataga
      781 ttcccttaga catgaataga gggatgacgt aagcaaaact tagaaataac ataaaaatga
      841 tatctattat tt
//
LOCUS       DAEGIQ010000290          847 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000290 DAEGIQ010000000
VERSION     DAEGIQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.305"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(37..237)
                     /locus_tag="KMT45_003062"
     CDS             complement(37..237)
                     /locus_tag="KMT45_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBE7006424.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            633..782
                     /locus_tag="KMT45_003063"
     CDS             633..782
                     /locus_tag="KMT45_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006425.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          272 a          145 c          134 g          296 t
ORIGIN      
        1 ttttctttcg aaaagacggt taaaagtcaa tttttattaa gctttaacaa tgtttgttgc
       61 ttgagggcca cgttgaccat cttcaacatc gtaagacact gcttggcctt cttctaaagt
      121 tttgaagccg tcaccttgga tagctgaaaa atgtgcaaat acatcatttc catcttcacc
      181 agtgataaag ccaaaacctt tgtctgcgtt gaaccatttt actgtaccgt tattcataaa
      241 taaattcctc ctgatacgta tcaatgtacg ttttttcgtt gcaataaata tttgataggc
      301 aaaaggagtt ttatctatgt agatatatcg ctcaaattac gtttcaaaat agattactca
      361 tttaatatat catgcttttt ctaataacac aaggataatg caatgaaata taaaattcat
      421 tgtcttctat tgtcttcgaa tcgaagaaaa agttgaataa ccgcgattga ttcacttttt
      481 gttttcttgt ttatttgctt ttatctgcaa cttttgactt taaaatttct taactatgaa
      541 aacctttcaa ttggtggtat acttataaat ggaatttcgt tatcaacgcg ttcgttgcct
      601 ttcagcaagt atttaaagaa aggagaatgg ctatgacatt ctataacaaa gatatacaat
      661 atcgagtaac cttggataca gatttgaatc tatttgtcgt ttttgacaaa gaaaatacca
      721 atcatgtagc gacaggtgtt acgatcgaac aagcagtgca agaattaaaa aaatctgcat
      781 aaggagtgga aaccctcata tggacgattc tcgttcttct ctttaaacgc atctactggt
      841 agataac
//
LOCUS       DAEGIQ010000291          805 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000291 DAEGIQ010000000
VERSION     DAEGIQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.306"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            218..487
                     /locus_tag="KMT45_003064"
     CDS             218..487
                     /locus_tag="KMT45_003064"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7006426.1"
                     /translation="MKLLNIENISKNYGDKAILENVSCEIESGEIIGLLGLNGQGKST
                     FVKIILDLLNKDSGHIDRFLSLKKKSRCDVAGSFFTGTFKSERVD"
     gene            435..>805
                     /locus_tag="KMT45_003065"
     CDS             435..>805
                     /locus_tag="KMT45_003065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE7006427.1"
                     /translation="MLQEVSLPEHLKVKELIELIRKFSDNPRTVKDALDMMDLGGFAN
                     YDCGKLSGGQKRKLQFSVAITNNPSFLVLDEPTARMDIVSKELFWTNIKKMLKMQNLT
                     VLLISHDLLEVESVTSRIIIL"
BASE COUNT          293 a           87 c          151 g          274 t
ORIGIN      
        1 aagtaaggag atgagtaaat gttatcaaaa agtaatcgca aatattgttt tatgttttta
       61 ggattattga ttggtttagt ttatagtatt ttccttattt ttacctagaa tggaggtgtt
      121 ataaatatgg ttaaatttga aaataatatt tttaaaaaat ttgtaactat tgatattaaa
      181 ctcatttaaa atatcgagaa agataaggag aaaaaaattg aagctgttaa atattgagaa
      241 cattagcaaa aactatggag ataaagcaat acttgaaaat gtctcctgtg aaattgaatc
      301 tggagaaatc attgggttac ttggtttaaa tggtcaaggg aaaagcacat ttgtaaagat
      361 aatactagac ctgcttaata aagatagtgg gcatattgat cgttttcttt cgctgaaaaa
      421 aaaatctagg tgtgatgttg caggaagttt ctttaccgga acatttaaaa gtgaaagagt
      481 tgattgagct catcagaaaa ttttctgaca atccaagaac tgtaaaagat gctcttgata
      541 tgatggattt aggtggtttt gcaaattacg attgtggaaa gctctctggt ggacagaaac
      601 gaaagcttca attttctgtt gctataacca ataatcctag ttttcttgtc ctagatgaac
      661 cgacagcaag aatggatata gtttccaagg aacttttttg gactaatatt aaaaaaatgt
      721 tgaagatgca aaatcttact gtcttactaa ttagtcatga tttattggaa gtagaatctg
      781 ttacatcaag aattatcatt ttaaa
//
LOCUS       DAEGIQ010000292          795 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000292 DAEGIQ010000000
VERSION     DAEGIQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.307"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            7..624
                     /locus_tag="KMT45_003066"
     CDS             7..624
                     /locus_tag="KMT45_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006428.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          309 a           97 c          146 g          243 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattt
//
LOCUS       DAEGIQ010000293          789 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000293 DAEGIQ010000000
VERSION     DAEGIQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.308"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            28..696
                     /locus_tag="KMT45_003067"
     CDS             28..696
                     /locus_tag="KMT45_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBE7006429.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          225 a          130 c          134 g          300 t
ORIGIN      
        1 aaattttcaa aagaaaagag gctagaaatg aaaaaattaa atttatttac agaattgtgc
       61 ggaatcattt taggcgcagg aatctacgga ttagctgtga caggaatcaa tattccatct
      121 aagctggcag atggtggggt tactggtatt gctttgcttt tgcataatct ttttggattc
      181 gctccttctg tcacttcact gctttttaat ttacctttat tgcttatctc attgtttctt
      241 tttggaaaac acgcttttct ccggacgatc gtcggaacat ttgcgctagt tttcttttta
      301 catttatggg agagccttca tatacatttt gctgtagata gtctactgat caatagtttg
      361 atgacaggta ttctttctgg cattggttgc ggtctcgtct ttcgattcgg cggaagcaca
      421 ggcggaacag atatcattta tcaagccatt gaaaaatatt ttcatattaa gattggcaaa
      481 agtcttttct tgattacttt tggtatttta gttgtctcct tgctttattt ggatctcaca
      541 cattttctgt atacattact tagctgttct attctatctt atacattgaa taaagtgaag
      601 tatctggaaa ttggatctgt tttgccccaa ttggaagcaa aacagactac agaaacattt
      661 gaaccacttg atcctcttga acagttagag cactgattct tatttttcac tactttattt
      721 aatttacaga aatatcatta tgtaaactaa aatgatatta ttcagtaaaa tcttctttat
      781 aaaaaaata
//
LOCUS       DAEGIQ010000294          784 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000294 DAEGIQ010000000
VERSION     DAEGIQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.309"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            102..773
                     /locus_tag="KMT45_003068"
     CDS             102..773
                     /locus_tag="KMT45_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE7006430.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT          312 a          117 c          120 g          235 t
ORIGIN      
        1 tcttttattt ttataactag tgtattactt caaaatttga tattatatgg catgaacaga
       61 attaaaatat cagttaaaat aaaagttaag gagcattgtg tatgacaatt aaaactagtc
      121 tccaaaatca agcttaccaa tctattcgcc gacaaattat ctatgctgac ttagaacctg
      181 gaaaaaaagt atcagaaaaa aaattagaag aaacactttc tattggtcgt acaccaatac
      241 gtgaagcact gattcaactc cgacaacaag gtcttgttga tacaattcct caaagcggaa
      301 attatatctc attaattgat ttagattcgg ccaaaaatgc tcgatttgtc agagaacatt
      361 tggaacgaca aattatgtta gagtgttgtg ccaaaatgaa taaccaaaaa aaacggattt
      421 tagaaactat aatttcagaa caagaaaaag ctgttagtca aaaaaatgct cgtagttttt
      481 ttcataatga taatttattc cataaagctt gttttgaaat tgcagagcga gaagaagtat
      541 ggcaatggtt agaaaataac aatacacact tagaacgatt tcgatggcta cgagtaaaaa
      601 cggaaggatt aaagtgggaa aaaattatgg aacaacatta tcaactattt gaggcattaa
      661 caaaacaaga tccagaagaa gctaactttc taacatcgtt gcatcttcat ttaatgctta
      721 atgagcaaaa tattgtttta gctactcatc caaaatattt caaaaatata taacttttaa
      781 ctct
//
LOCUS       DAEGIQ010000295          780 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000295 DAEGIQ010000000
VERSION     DAEGIQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.310"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>780
                     /locus_tag="KMT45_003069"
     CDS             <1..>780
                     /locus_tag="KMT45_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBE7006431.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          277 a          152 c          140 g          211 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
//
LOCUS       DAEGIQ010000296          757 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000296 DAEGIQ010000000
VERSION     DAEGIQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.311"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..645
                     /locus_tag="KMT45_003070"
     CDS             <1..645
                     /locus_tag="KMT45_003070"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBE7006432.1"
                     /translation="NYWRSFIETIIEERNKIVVLYNSVEEQPYLYSDKSTDIIFLGAV
                     IERKGIIDLIDAIDLIRNKIPQKWRLRIFGPTFNLDIEKIINDKNLHEYINYEGFIEA
                     ESIKDILSEVAFNVLPSYNEGLPMTILETMSFGIPSIATAVAAVPELVMHNANGVLQN
                     PGDVETLGNNLLLMMQNTELRSRFSKESYKTINEKFLLNTHVQKLLEIYKQVLD"
BASE COUNT          292 a           89 c          141 g          235 t
ORIGIN      
        1 aactattgga ggagttttat tgaaactatt atagaagaac gaaataaaat agttgttctt
       61 tacaactccg tcgaagaaca accatattta tattcggata aatcaacaga tataattttt
      121 ctgggagcag ttattgaaag aaaaggcata attgacttga tagatgctat tgatctgatt
      181 agaaataaga taccacaaaa atggaggtta cgaatatttg gccctacatt taatttggat
      241 attgaaaaaa taattaatga taaaaatcta cacgagtata taaattatga gggcttcata
      301 gaagctgaaa gtattaagga tattctaagt gaagttgcgt ttaatgtatt gccatcttat
      361 aacgaggggt tgcccatgac tattctagaa actatgtcat tcggtattcc aagtatagca
      421 acagcagtag ccgcagttcc agagttggta atgcataacg caaatggtgt tttacaaaat
      481 cctggagatg tggagacatt aggaaataat ctattactaa tgatgcaaaa tactgaactt
      541 agaagtagat ttagtaaaga gtcatataaa acgattaatg aaaaattttt gttaaatacg
      601 catgtgcaaa aattattgga aatttataaa caggttttgg actgaaaacg catgaataac
      661 ttagggtttt atattgtata aggaattaac ccttataacg atatattttt taaggtatca
      721 gagctatagg taccaaaaat aatgattaga agaaatg
//
LOCUS       DAEGIQ010000297          720 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000297 DAEGIQ010000000
VERSION     DAEGIQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.312"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            1..720
                     /locus_tag="KMT45_003071"
                     /pseudo
     CDS             1..720
                     /locus_tag="KMT45_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          279 a          120 c          135 g          186 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
//
LOCUS       DAEGIQ010000298          717 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000298 DAEGIQ010000000
VERSION     DAEGIQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.314"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(74..649)
                     /locus_tag="KMT45_003072"
     CDS             complement(74..649)
                     /locus_tag="KMT45_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBE7006433.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          240 a          153 c          126 g          198 t
ORIGIN      
        1 cttaatttga caaataataa aatgaaaaaa cagagccaga ttagcattat tttgctaatc
       61 cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgg ggcaacatag
      121 tgatgactaa tgaaacaacg acgatagaca acaagcgatc aaggtagtct gtacccgcct
      181 gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc tgaacgatca
      241 tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc gaagaaacga
      301 tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg tttttatctt
      361 tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt tggattggcg
      421 aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg gacaatcctg
      481 tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg atagaatcga
      541 gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg atcccaatac
      601 acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt ttgttagccc
      661 atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DAEGIQ010000299          712 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000299 DAEGIQ010000000
VERSION     DAEGIQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.315"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            44..379
                     /locus_tag="KMT45_003073"
     CDS             44..379
                     /locus_tag="KMT45_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006434.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT          270 a           80 c          119 g          243 t
ORIGIN      
        1 aatttcctag gaaaaatagt taacataaaa ggaatgaatt gttatgaaac aatatttaaa
       61 tgaatggaaa gcgatagagg ggtcattagt aacgcaaaga ataaaggaat tacctgattg
      121 cttagagaag gatcatttgt ttcaaatacg agaaatgttg aaaaaagaac agtttgatcc
      181 agaccaattt ttagtagtcg agtatccaac tattggagtg tactgttgta accatatcaa
      241 tgatggaaaa tattttatta ttcaggagta tggaggacag ttaactcctt tctatactac
      301 atgggaaatg agtgaagaag gaatcaataa ttttccatgt gaaagcatcg aagattcaat
      361 ttcacacacc gaatgttaat tgattataac tataaaaaag gattgaacat tatgttcaat
      421 ccttttttca atgaaatgtt ttccgccatt tcattgtttg gtgcgatttc tattttatta
      481 tatcaaaata aagagagatt ctaaagaaag aagagggaaa tatataaatc aagcgacagt
      541 atatttatta ttaaatgcta ttgtgagaat ataactaatt agtttaattt gaaagtaaat
      601 atacgctata aaagtgcatc ttgtgttata aaaaattatt ttattttgtg aacataattt
      661 aacaattctt tctcggaatt gatttctttt cttaaatagt cttcagactt at
//
LOCUS       DAEGIQ010000300          679 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000300 DAEGIQ010000000
VERSION     DAEGIQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.316"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>679
                     /locus_tag="KMT45_003074"
     CDS             <1..>679
                     /locus_tag="KMT45_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBE7006435.1"
                     /translation="LEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVG
                     RAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDY
                     SQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFES
                     VPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLL
                     KSMDFNSV"
BASE COUNT          229 a          114 c          137 g          199 t
ORIGIN      
        1 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
       61 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      121 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      181 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      241 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      301 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      361 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      421 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      481 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      541 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      601 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      661 atggatttca attcagtag
//
LOCUS       DAEGIQ010000301          672 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000301 DAEGIQ010000000
VERSION     DAEGIQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.317"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..448)
                     /locus_tag="KMT45_003075"
     CDS             complement(<1..448)
                     /locus_tag="KMT45_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7006436.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          198 a          119 c           90 g          265 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat ta
//
LOCUS       DAEGIQ010000302          672 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000302 DAEGIQ010000000
VERSION     DAEGIQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.318"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            77..478
                     /locus_tag="KMT45_003076"
     CDS             77..478
                     /locus_tag="KMT45_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBE7006437.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            472..>672
                     /locus_tag="KMT45_003077"
     CDS             472..>672
                     /locus_tag="KMT45_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7006438.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQL"
BASE COUNT          243 a           97 c          128 g          204 t
ORIGIN      
        1 ttttctagtt ttttcgaaaa aaaggggctt tctgattgtt gtattagtgg aaagggggaa
       61 aaaggagcat attatcatgc aacaacatta caatgacata tctatacaac accaatttga
      121 tgcctactgt aaaaaagtat tacgaaacga agctaagtct attcgaaaac gaaacgctaa
      181 cataaaggat aatgaggaac cattggcaga ttcaaatgaa ggcaggtatt cccaaactca
      241 ttttgatgaa caagatgtct atttcttatt tggaatggaa atattaattt cagatcaaaa
      301 gttatcagca gcaattgatc agttatcgga gataagaaga aagattatcc tactttatta
      361 ttttgcaggt tttaatgatg ctgaaatcgg taaaattctc aatatgagca caagtggtat
      421 ctggtatcaa cgaaagaagg cagttgaaca attgaaaatg gggtatgacc tatggtagat
      481 aaatctaaaa agcggcagta cctattagtt ccactatcca ttatcttatc tgctctagaa
      541 ggagatccaa tttcgatcaa gatgatactc aaacattatc attcttatat ggctaagtta
      601 tgtttgacaa atggatttaa cgaggtaggt caattcgtta cctatgtaga tgaatatatg
      661 ataagtcagc ta
//
LOCUS       DAEGIQ010000303          649 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000303 DAEGIQ010000000
VERSION     DAEGIQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.319"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            31..>649
                     /locus_tag="KMT45_003078"
     CDS             31..>649
                     /locus_tag="KMT45_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE7006439.1"
                     /translation="MLKKERQERIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVAD"
BASE COUNT          244 a           99 c          115 g          191 t
ORIGIN      
        1 attgactttt atgttaaagg agtaattcat atgctgaaaa aagaaagaca agaacgaatc
       61 attacgttat tagagaaaga tttttattta actaattcag aaatcgctaa caaattaggc
      121 gtatcagaaa tgaccatacg ccgagatgtc actgaattag caaaaaaaaa taaagtaaaa
      181 aagctatatg gtggcgttga acgaaaggaa ccttataatc gagaactatc gactcaagaa
      241 aagattgata cgaatgtagc cgaaaaaaaa tatattggtc aagtcatgaa cagtatgatt
      301 cccgataatt ctactattta tgtcggtgct ggaacaacga tcctatatgc actccctttc
      361 attcaaaaga aaaatctatt tgtaattaca aatagtttga ttgctttcaa ctatttaaag
      421 aatcatacaa gttatcgaat tttacttact ggtggcgaat tttctcctat tacagaagag
      481 ttcattgggg aagttgcaga aagatctttt gaaagtttga atgtcgatat ctctttcgct
      541 gcaacaaacg gcatatttga taacaatgtg acaaccgctc aatttattga gggaaatatt
      601 cagaacgcag cgctcaatgc agcaaaaatc aaatgtattg ttgcagatt
//
LOCUS       DAEGIQ010000304          624 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000304 DAEGIQ010000000
VERSION     DAEGIQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.320"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>624
                     /locus_tag="KMT45_003079"
     rRNA            <1..>624
                     /locus_tag="KMT45_003079"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          181 a          136 c          181 g          126 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctg
//
LOCUS       DAEGIQ010000305          614 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000305 DAEGIQ010000000
VERSION     DAEGIQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.321"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(217..582)
                     /locus_tag="KMT45_003080"
     CDS             complement(217..582)
                     /locus_tag="KMT45_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006440.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          237 a           85 c          112 g          180 t
ORIGIN      
        1 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
       61 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      121 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      181 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      241 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      301 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      361 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      421 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      481 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
      541 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
      601 aagtgttatt attt
//
LOCUS       DAEGIQ010000306          524 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000306 DAEGIQ010000000
VERSION     DAEGIQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.323"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(152..346)
                     /locus_tag="KMT45_003081"
     CDS             complement(152..346)
                     /locus_tag="KMT45_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006441.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT          163 a          110 c           79 g          172 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caat
//
LOCUS       DAEGIQ010000307          509 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000307 DAEGIQ010000000
VERSION     DAEGIQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.324"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..132)
                     /locus_tag="KMT45_003082"
     CDS             complement(<1..132)
                     /locus_tag="KMT45_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBE7006442.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAEGIQ010000308          494 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000308 DAEGIQ010000000
VERSION     DAEGIQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.325"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            1..268
                     /locus_tag="KMT45_003083"
                     /pseudo
     CDS             1..268
                     /locus_tag="KMT45_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          184 a           73 c           87 g          150 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atcgccgatt gtaaaattaa
      361 gctagacaac taaaaaagtc atttgtgcta cactcaaaat agttccgacc aaagaattat
      421 aaggagtgag tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag
      481 tcttatttca aaat
//
LOCUS       DAEGIQ010000309          467 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000309 DAEGIQ010000000
VERSION     DAEGIQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.326"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..344
                     /locus_tag="KMT45_003084"
     CDS             <1..344
                     /locus_tag="KMT45_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HBE7006443.1"
                     /translation="ESEWVIKAREIERLEPDIVFVGITSPKKEYLIDYFRTQRLKSVF
                     MGVGGSFDVLSGKIKRAPKIVQNLNLEWLYRVYQEPKRLFKRYTIGNLIFLRMLVSEL
                     MNKKERKITDK"
BASE COUNT          192 a           41 c           93 g          141 t
ORIGIN      
        1 cagagagtga atgggtaatt aaagcaagag agatagaaag attggaacct gatatagtat
       61 ttgttggaat aacatctccc aaaaaagaat atttaattga ttactttaga actcaaagat
      121 taaagagcgt ttttatgggg gttggtggta gttttgatgt tttatctgga aagataaaaa
      181 gagcgccaaa aatagttcaa aatttaaacc ttgagtggct ctatcgtgtt tatcaagaac
      241 caaaaagatt atttaaaaga tatactattg gcaatcttat ttttcttcgt atgcttgtta
      301 gtgagctaat gaataaaaaa gaaagaaaaa taacggacaa atgaaaagga aatttgctag
      361 atgaaaaaaa taaatgtatt aatgataggg gttagtaaaa aaacaaaagg aggaatgtgg
      421 actgttgtta ataattattt gaatagtaat gactaccata ataatat
//
LOCUS       DAEGIQ010000310          467 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000310 DAEGIQ010000000
VERSION     DAEGIQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.327"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..365
                     /locus_tag="KMT45_003085"
     CDS             <1..365
                     /locus_tag="KMT45_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006444.1"
                     /translation="DPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKE
                     LVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAET
                     LDQLLPWNKDVQQHFSRN"
BASE COUNT          150 a           86 c           93 g          138 t
ORIGIN      
        1 tagatcctgt tgcaaaatcc aagttggatg cagccgttca atatgcgtgt aaactacgaa
       61 gtggttttga acctattttt gaagatggtc gtttagaact cacaaataat cgtgcggagc
      121 gaaatatcaa agagctggtg ataggacgga aaaattggtt gcattccaca agtcttgaag
      181 gagcacgaac ttcaggaatc attctgagtg tatataaaac agcagaactt aacggattga
      241 atcctgtaaa gtatctagag tttctatttg ataagatacc aaatcttccc gtactttcag
      301 cagaaacttt agatcaactg ttgccttgga acaaggatgt acaacaacat ttttctcgta
      361 actaaaaacg ccaaacattc ccgattctat tctataggtc gggttatttg acgtacaggt
      421 actgttctat ggggcgctta cttttcttct tcaagaccaa ataaaaa
//
LOCUS       DAEGIQ010000311          459 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000311 DAEGIQ010000000
VERSION     DAEGIQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.328"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            11..>459
                     /locus_tag="KMT45_003086"
     CDS             11..>459
                     /locus_tag="KMT45_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE7006445.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYLK"
BASE COUNT          179 a           59 c           90 g          131 t
ORIGIN      
        1 ggtgcttttt ttgcggaaat atacatttgt ttttaagaaa aaagtagttt cagactattt
       61 aaataacgaa ggcggctaca aatatcttgc acataaatat caaataaatc gtacactggt
      121 tagacattgg gtaaggattt ataactatca tggttgggaa ggcttggttg gaggtggcaa
      181 aagctacact acaaaattta aacttgatgt tatagaatat atggaaacaa atggtctttc
      241 tatccaagaa actgctaaaa aatttaatat cggttcaaat agaactctaa gtaaatggat
      301 agagcaatat gaagaaggcg gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat
      361 tagtatgaat tccaagctaa acattcctaa aaaacttaaa gatgagtctc ttgaagaaga
      421 agttattcgt ttaagagcag agaacgcata tttaaaaaa
//
LOCUS       DAEGIQ010000312          456 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000312 DAEGIQ010000000
VERSION     DAEGIQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.329"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          146 a           87 c           52 g          171 t
ORIGIN      
        1 aaaataaaaa aattattacg ttattaaaaa aacagatgaa agatcaggtt ttattagatc
       61 tcattctttc atcacttgaa gggatttaat gaactacttt ttcctctttg tcgtttttat
      121 atattcgtat tccttcaaac agtaggatag cctctttcaa tgcttgatat ttgaactgtc
      181 cgttacttaa aaatttagat gtgccgccct cttcctagaa catattcttt tttacttttt
      241 cattaattga cagaccaatc gaacgaaact gtaaatttcg ataggataat gattggtcaa
      301 actcattcat tttcaagaca tctcgattca tattcaagat aggaatcttt ttatcaacat
      361 cactttattc gtattttcct ctaccataaa agcttcacgc tccacaaaat tgttttatac
      421 actactattc agcccattca acgaccaatg aatttt
//
LOCUS       DAEGIQ010000313          451 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000313 DAEGIQ010000000
VERSION     DAEGIQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.330"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>451
                     /locus_tag="KMT45_003087"
     CDS             <1..>451
                     /locus_tag="KMT45_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006446.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAEGIQ010000314          446 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000314 DAEGIQ010000000
VERSION     DAEGIQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.331"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(188..>446)
                     /locus_tag="KMT45_003088"
     CDS             complement(188..>446)
                     /locus_tag="KMT45_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006447.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          155 a           59 c           79 g          153 t
ORIGIN      
        1 acaggaattt atcgaaaatg gtagaaaaga aatttataaa aggacttata aagattgatt
       61 atataatata tgaaaagcta ttataaaaga cattagtatt aaatagttta aaaaaatgaa
      121 aaataataaa ggaagtgagt caagtccaga ctcctgtgta aaatgctata caatgttttt
      181 accatttcta cttatcaaaa ttgatgtatt ttcttgaaga ataaatccat tcatcatgta
      241 ggtccataag aacggctcca attaagcgat tggctgatgt ttgattgggg aagatgcgaa
      301 taatcttttc tcttctgcgt acttcttgat tcagtcgttc aattagattg gtactcttta
      361 gtcgattgtg ggaatttcct tgtacggtat attgaaaggc gtcttcgaat ccatcatcca
      421 atgatgcgca agcttttgaa tatttt
//
LOCUS       DAEGIQ010000315          436 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000315 DAEGIQ010000000
VERSION     DAEGIQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.332"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>436
                     /locus_tag="KMT45_003089"
     CDS             <1..>436
                     /locus_tag="KMT45_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBE7006448.1"
                     /translation="KNLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYS
                     GLVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNV
                     FKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRD"
BASE COUNT          188 a           52 c           91 g          105 t
ORIGIN      
        1 aaaaacttag tgactgctgc gagagataac gataggttaa aacagcatgt tagaaatctc
       61 atgagtactg atatggcgag agaatataaa aaattaagta aagaacatgg gcaagttaaa
      121 gaaaaatata gtggtcttgt agagcgattt aatgaaaatg taaatgatta taatgagttg
      181 cttgaagaaa acaagtcttt aaagtctaaa ataagcgatt taaagcgtga tgtgagttta
      241 atctatgaaa gcactaagga attccttaag gaacgtacag acggcttaaa agcctttaaa
      301 aacgttttta aggggtttgt agacaaggta aaggataaaa cagcacaatt ccaagaaaaa
      361 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa
      421 gaacgaagtc gagatc
//
LOCUS       DAEGIQ010000316          430 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000316 DAEGIQ010000000
VERSION     DAEGIQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.333"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>430
                     /locus_tag="KMT45_003090"
     CDS             <1..>430
                     /locus_tag="KMT45_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBE7006449.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKT
                     GFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          145 a           81 c           74 g          130 t
ORIGIN      
        1 caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt
       61 aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac
      121 tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt
      181 ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt
      241 ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat gatcgttact
      301 aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga cgtgaaagtg
      361 ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa aggatatatt
      421 agccataaaa
//
LOCUS       DAEGIQ010000317          422 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000317 DAEGIQ010000000
VERSION     DAEGIQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.334"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..421
                     /locus_tag="KMT45_003091"
     CDS             <1..421
                     /locus_tag="KMT45_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7006450.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          157 a           65 c           86 g          114 t
ORIGIN      
        1 atcactagat cgtttaggaa gagattatga agatataaaa aatacagttg cttttatgaa
       61 acagaaaaaa gtagcgttaa aaatattaga tgcacagttt ttagatttta atactggaaa
      121 tgaactcctg gataccgcta tgttcgatat gtttttatca ctgctaagtt atattgcaca
      181 aaacgagcgt gagaaaattc gtgaacgtca gcggcaagga gtattattgg ctcaaaaagc
      241 cggccgatat aagggacgac caatcgagta ttctattaac tcaacagatc cgcaaaaacg
      301 cttagttttt aaaaccgtgg tggacatgtt aaagcaaggc attccagttg cagagattgc
      361 aaaagaaaac gggatctctc gaccaactat ttataaaata aagaaagcga atgatttgta
      421 ga
//
LOCUS       DAEGIQ010000318          412 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000318 DAEGIQ010000000
VERSION     DAEGIQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.denovo.335"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..310
                     /locus_tag="KMT45_003092"
     CDS             <1..310
                     /locus_tag="KMT45_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE7006451.1"
                     /translation="LTNVKPIKILIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRT
                     NITEVDLVISNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY
                     "
BASE COUNT          149 a           53 c           74 g          136 t
ORIGIN      
        1 tttaacaaat gttaaaccaa ttaagatttt aatacaagat gatttacacc atacgcatcg
       61 gttatggtta atgaataaaa ttaatttgta ttttgggcat tcttatgttt ttgcttttta
      121 tgattacagg acaaatataa ctgaagtaga tttggttatt tctaattact acatcgacac
      181 tggaaaaacg ccattactgt taatgaaaaa tatacctacc gaaagaaact ggcgtttgtt
      241 cgagaaaacg atttatcaat tgaaaaaaga aaaagaggtt gtgaaatcag cgttttgtcc
      301 tgagtattag agaaacaaac ggaaaaatag tttttcatat tttgccgttt ttgagctaat
      361 accgagggac agctgattga acaccattta ttgagaggtt gtgaaaaaac tg
//
LOCUS       DAEGIQ010000319        16343 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.058, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000319 DAEGIQ010000000
VERSION     DAEGIQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.058"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..218
                     /locus_tag="KMT45_003093"
     CDS             <1..218
                     /locus_tag="KMT45_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006452.1"
                     /translation="AKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLW
                     FIPVALCAAEMATVKGWEKGVSQTKRY"
     gene            613..1086
                     /gene="dfrF"
                     /locus_tag="KMT45_003094"
     CDS             613..1086
                     /gene="dfrF"
                     /locus_tag="KMT45_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBE7006453.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            1213..1869
                     /locus_tag="KMT45_003095"
     CDS             1213..1869
                     /locus_tag="KMT45_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBE7006454.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            2051..2581
                     /locus_tag="KMT45_003096"
     CDS             2051..2581
                     /locus_tag="KMT45_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBE7006455.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            3127..3369
                     /locus_tag="KMT45_003097"
     CDS             3127..3369
                     /locus_tag="KMT45_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBE7006456.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            3366..3836
                     /locus_tag="KMT45_003098"
     CDS             3366..3836
                     /locus_tag="KMT45_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBE7006457.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            3964..5178
                     /locus_tag="KMT45_003099"
     CDS             3964..5178
                     /locus_tag="KMT45_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBE7006458.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            6167..6376
                     /locus_tag="KMT45_003100"
     CDS             6167..6376
                     /locus_tag="KMT45_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBE7006459.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            6488..8101
                     /locus_tag="KMT45_003101"
     CDS             6488..8101
                     /locus_tag="KMT45_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7006460.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            8136..9347
                     /locus_tag="KMT45_003102"
                     /pseudo
     CDS             8136..9347
                     /locus_tag="KMT45_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            9370..10296
                     /gene="glsA"
                     /locus_tag="KMT45_003103"
     CDS             9370..10296
                     /gene="glsA"
                     /locus_tag="KMT45_003103"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBE7006461.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            10504..11964
                     /locus_tag="KMT45_003104"
     CDS             10504..11964
                     /locus_tag="KMT45_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006462.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            12185..13948
                     /locus_tag="KMT45_003105"
     CDS             12185..13948
                     /locus_tag="KMT45_003105"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBE7006463.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            14032..14490
                     /locus_tag="KMT45_003106"
     CDS             14032..14490
                     /locus_tag="KMT45_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBE7006464.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            14599..15282
                     /locus_tag="KMT45_003107"
     CDS             14599..15282
                     /locus_tag="KMT45_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBE7006465.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            15429..16043
                     /locus_tag="KMT45_003108"
     CDS             15429..16043
                     /locus_tag="KMT45_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBE7006466.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            16059..16265
                     /locus_tag="KMT45_003109"
     CDS             16059..16265
                     /locus_tag="KMT45_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006467.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         5717 a         2511 c         3410 g         4705 t
ORIGIN      
        1 ctgcaaaaaa attaacgttc tttggctttt tggctatgac catctcaatg gtcgtttcat
       61 tatatgaata tcccacattc gcgacctcag gcttcagctt agtatttttc ttgttattag
      121 gcggtttgct atggtttatc cctgtggcat tgtgcgcagc agagatggca acggtgaaag
      181 gatgggaaaa aggtgtttcc caaacaaaaa gatattaaga aacagcccat gtttacctgc
      241 ttgcaaacaa aagaatattg ctactagact aagagatact acataagtgt gttggtatct
      301 cttttttgtt gccgtgaaac agcagactat ttcatttctg cttgattacc ttaaaactta
      361 ttagtctatg gtttgtcaag ggcttgttgc gtgagaaatg ctgaatatca ccctttacaa
      421 aacataggct gataagtaac ttttcaaaag cagaaaagaa atggcaatgc ttataacaat
      481 atgatataat gcagatgaag acaaaaaagg aatttgtcga gctggttagt tcgagataat
      541 tttgagtttg ttaagatgtg gtgcacttga tggaatcaca ggtacgacat catattatgg
      601 aggatgaaat agatgatagg tttgattgtt gcgaggtcaa agaataatgt tataggcaag
      661 aatggtaata taccatggaa aataaaggga gaacaaaagc aatttagaga gttaacaacg
      721 ggtaatgtgg ttattatggg gcgaaagtct tatgaagaaa tcggtcatcc gttgcctaat
      781 agaatgaata ttgttgtttc caccacaaca gagtatcaag gagataattt agtttcagtt
      841 aaatcattag aagatgcatt attattggct aaaggacgag atgtatacat atctggtgga
      901 tatggactat ttaaggaagc tttgcaaata gtagataaaa tgtatatcac agaagtagat
      961 ttaaatattg aagatggaga tacattcttt ccagaatttg atatcaatga ttttgaagtt
     1021 ttgatagggg aaacacttgg tgaggaagtg aaatatacga gaacatttta tgtaaggaaa
     1081 aaatgaattg agtaaatttt ggatttaggg tgttttcata aatatattgc tttttatgca
     1141 tatataaatt gttgtaagac aaattccagt ttgtatgggg cgataaaatc agaatttgag
     1201 agaggattga atatgaataa ggaatggtct gaacttaata aaacaatgca ggcacaaata
     1261 aaaaagaaag atacttataa gaggggtatt gatactttac ttactttacg aagtcagtta
     1321 atacaaacct tagtatcttt caaagaagaa ttatgcagag aagattttaa ctcaatcccc
     1381 ttcataaacg ctgatggtta tcatagtaag acgattgctt attctatttg gcatattttc
     1441 cgcattgaag atatagttgt gcatacagtg ataaatgaag atgaacaagt attttttgca
     1501 ggcaattatc aagagcgtat caattcacct atcattacaa caggaaatga actcatgaaa
     1561 cagcagattg cagacttttc aaaacagctt aatctggaag aattatattt atatattttc
     1621 gaagtatggg aaagcactga gaaaatgctt gaacggttat cttatgatga attgaaaaga
     1681 aaaataccga aagaaagaaa aggatactta gaatcgttga atgtagtaaa cgacaatgaa
     1741 aaagcaattt ggctgattga ttattggtgt aataaagata tttgtggact aattcaaatg
     1801 ccgttttcaa gacattggat tatgcacaca gaagcctgtt tgcgtataaa gaacaagata
     1861 cattcatagg caaaaatttc agtttgtaga actaaggaaa ttggaattta aggaggaata
     1921 aagtatggtt agtgatttta taagagcagc atttccatgg attttaatgg gtttatttgt
     1981 agcaatcagt tgttccctta tgagtaaaaa agagaaataa attcctgttt gtatttataa
     2041 aagagaaaaa atgagaaaag tagttttatt tattgccatg agccttgacg gatatattgc
     2101 ggacggtaat ggtggagtag cttggctaaa tggtcatgga aatgacaatg aaaacatcga
     2161 cacttataca gaatttacca aagatataga tactgtctta atggggtgga atacttctca
     2221 tcaggttgtt actgaacttt ctccgcaaga atgggtatat aataaattta caacttatgt
     2281 attaacacat aaagagtgca attcttccca acaaattcat tttacaagtg aaaatcctgt
     2341 tttattattg gaacggctaa aacaagaagc aggaaaggat atttggattt gtggcggagc
     2401 aaatcttgtt cagcagttgg taagcaaaaa tatgattgat aaatattaca tttctgttat
     2461 tcctactctg ttaggaaatg gggttcgtct tttgggcaac atagacaggg aaataaagtt
     2521 aaggctttgt aaaacacaga cttataacgg aataaccgat ttgatttata tgcgtagata
     2581 actagagaga aacagaaaag ctgttaaacc gacggagcga tccgaccacg ttggcgggaa
     2641 aatccttaag atttttccgc catgcttgca agggggcggt tcgcttcggc ggttcggcga
     2701 gcttgcgagc cgttcaaagc ccccgttatc taacaagggg ggcacaaaaa acaagaaaca
     2761 ctatacacta acaagcggaa aacgctgtat ttataaggca aaaccccatt tttacagtag
     2821 tgatataaat tgtacctata tcactatgaa aattgaatag taaatatgag aaagacagtt
     2881 gatttcaaaa aataagaaat tgattgtcgt tttcttttgc agaaatttaa gtgcaggcaa
     2941 ttatggtagt tgtcgtaatt gctgtcagct cagtgtttat cactgatggt gtgaaagatt
     3001 tacggttgga gatgttcaat aagattattg gtgcttaatt atttttattg tagtgctgat
     3061 atgagaatgt tataattggg gtataaaaca tacaaataaa tttgctgaaa aatgaggtat
     3121 caaaatatgg aaaatgaatt actggaaaac ttaggaagac ttcacacaac tgaacttggc
     3181 gttattagaa ttaaaaagaa cttatctttg aatgtagaaa atgtaattga atggtgcaaa
     3241 gaaaaaatta gcctttctaa tgcggaaatc acaagaaaag gaaaaaactg gtatataacg
     3301 attgataatt gtattataac ggtaaatgcg tacagttata ccgttatcac tgctcacaag
     3361 gtaaaatgat aaatttttct ataaaggaat taaacgcatt tgcagttatt gggcaggaag
     3421 tagaactgac taattatcaa aaaaagaata ttcagattag tacacagttt tggagaaagt
     3481 ttaacagtag tttgaaaaaa gcgtatcttt cacaatcagg aaattgggta aaatatgctt
     3541 ttatggaaag aagaaatgga aaactttatt atttctgttc tattccccaa agaaccatta
     3601 ccccagaggg ctttctgtat aaagaaatac cgtcttataa atatttggtt gtggagcata
     3661 ttggtgctat ggataaaata tatgaaactt atggcaagat ttatgaagaa attattccta
     3721 gtacatcgta tattcctata aaagatatta tcctacactt tgaaaaatac gattatcgtt
     3781 ttcattggaa tagtgataat tcagttattg aaatttggat acctattcaa gattagaagc
     3841 aattttatat ccatacacac aaagcagtta gtcttaggat tgactgcttt ttctatacag
     3901 atttttaatg acaggcaatt atcaattatg gtagttgcct tttttgattg gaggaattga
     3961 agaatgaatg ataaaaactt tattgaagaa ctaagacaaa agcgtgagga atacggagta
     4021 acacaaacaa ggcttgctgt tgcctgtggt atcagccgtg aatattacaa ccgcattgaa
     4081 aaaggaaaac agccattgaa tgacgaatta agagaaatca tagaaaaaca gattgagcgt
     4141 ttcaatccgc aagaaccact attcttattg attgattact ttcgtgtccg ctttcctact
     4201 acggacgcat tggcgattat ccgtgatgta ttacaactga aatctaatta catgctttat
     4261 gaagattatg gaaaatacgg atatgaaagc aaatatgtac ttggcgacat caatatcatg
     4321 tgttccatgc aggagcattt aggtgtttta ttggaactga aaggcaaagg ctgtcggcaa
     4381 atggaatgtt atctgctggc acaggaacgc tcatggtatg acttcatgct ggattgtatg
     4441 acggctggtg gtgtgatgaa acggcttgat ttggctatca atgataaagc aggaatattg
     4501 gatattccaa agttaaagga aaaatacaag gctggcgaat gtatgtccta cttccgtatg
     4561 cagaaagatt acagcggtac agaaaaatgc ggtagcgatt taccaaagaa tacaggagaa
     4621 accttatatc tcggttcaac aagtagcgaa ttatatatgt gtgcttatca gaaaaattat
     4681 gagcagtatg tcaagaatgg cacagaaatt gaagatacgg agattaagaa ccgttttgaa
     4741 atacgcatga agaacgaacg agcctattat gcggttgtag atttactgac ctatcgggac
     4801 gctgaacgca ccgctttttc tatcatcaat cattatgtcc gctttgttga cagagaggac
     4861 gacaagccaa aaagtcaatg gataacaaat gatgattggg catggtttgt aggggaaaac
     4921 agagagccga tacgcttaac cacaaagcca gagccttaca ctttacaaaa agcgttgcat
     4981 tggctacaaa gacaagtcgc accaaccata aaaatggtac aggcattaga cagagaaaat
     5041 cgtacaacga tactgaaaga tatgattgag caggcagaac taaaagacaa gcataaacat
     5101 ttattacaat tagagaaatc aaccatagaa gaacgtatag ataccgctgt tccacaagag
     5161 aatgacggta ttttttaatg taattgaaaa atttcagatt tagtcagcaa agagctgttt
     5221 tcattcccta tgtgaagtga aagaagaaaa ataaattttt tattaaatct gcaacaaaat
     5281 ggctacttgc gtacagatat ggtgcgagaa aaggaaatct aaactttttt tgaaaatagg
     5341 tcattaaatc ggggcttgct gtccctatat gaagtgaaag aagaaaaaag ttttgaaaaa
     5401 atgacggaaa ttcgtgatta gtggacagtc ttctgtgaaa agaggaaaaa catcatcatt
     5461 ttgtattcaa acaggcatta gcagttgagt gttgatgtga aaagatgaaa atactttgtt
     5521 ctcaacttaa caatttaagg tttccgttcc ctatatggtg caaagaaaat atttcattct
     5581 attgttggca gtaacggtat ggtttgggta gttagggtgt gaaaaggaaa atattctttt
     5641 ttcatcatca acttagcaaa atgacatttt ctatccctat atggtgcatg aaaagaagtt
     5701 aaaatttttc catttaaact tagcaaatgg acgtttgctg tacagatagt agtgaaaaag
     5761 gagaaaatca accgttgatt gccaaaatga accgttccat ttgtactaat aatggaaaaa
     5821 gttttggatt ttggttacgt ttctcctctt ttccaacgcg atatatagga aagacgattt
     5881 ttttctttca atcgttcttt gacaactgaa taaagctatt caatacgttt gacagacagc
     5941 tagccgttga aacaaatacg ccacgacctt tcccctgcaa gagcgagcga aaacctattt
     6001 gtgttcttgt aaaagatttg tcctctttta tcgccatgac ctgtactgtc agataatgat
     6061 actcccgtac agccacagct tgagcattaa gagcgtcgca cgcaatgggt acggctacat
     6121 aagaactatg cagaggtgga actcctgtgg gctatgacaa aagccgttga attgcttaga
     6181 aagattttac agaaaggggg tatgtgttat tgataatgct gtaaaaacaa ttcaaaagaa
     6241 aaatggcaag tgtggcattg gcaacagagg aaagacaaag attgtagtaa aagagcattt
     6301 ttccgaaaag ggcaaaacaa tggaagaact tctaactgat gtcatgctgg aaaaagcaaa
     6361 gcagacaatc gcataaaatc ggtgcagttg taagaaatag attgaatgac aaaaagtacc
     6421 cgtgttatac tttacttgca agcaggtatt gtaaacacgg gttagttata aaggaggata
     6481 actgacaatg aaacaacaga tttacaatac tgcactttat cttaggttaa gccgagatga
     6541 tgaattacag ggcgaaagtt ccagcattac cacacagaga agtatgttgc gtttatatgc
     6601 aaaagaacat catttgaatg tcattgatga atatattgat gacggctggt cgggaacaaa
     6661 ttttgacaga ccgagttttc aaagaatgat tgaggatata gaggcaggaa aaatcaactg
     6721 tgttgtaacg aaagaccttt cccgtcttgg cagaaattat attatgacag gacaatatac
     6781 agaattgtat ttccctagtc ataatgtccg ctacatagcg attgatgacg gtgtagacag
     6841 cgaaaaaggc gaaagtgaga ttgcaccatt taagaacatc atcaatgaat gggtggcaag
     6901 agatacaagc cgtaaagtca aatcagcatt taagacaaag tttgcggagg gggctcatta
     6961 cggtgcttat gctccgttag gatataagaa acaccctgac atcaaaggaa aactgttggt
     7021 tgatgatgaa acaaaatgga ttattgaaaa aatcttttcc ctagcctatc aaggttacgg
     7081 aagtgcaaaa atcacaaagc agttaagagc agaaaaagtt ccgacagcgt catggctaaa
     7141 ttttacaaga tacggtactt ttgcacatat ctttgaggga aaacccgaaa gtaagcgtta
     7201 tgagtggacg attgctcatg tcaaggcgat attgaaaagt gaggtttata tcggaaacag
     7261 tgttcacaat atgcagtcta cggtatcgtt caagagcaaa aagaaagtgc gtaagcccga
     7321 aagtgaatgg tttcgagtag aaaacactca cgagccgatt attgacaagg aagtgttcta
     7381 tcgtgtgcag gagcagataa aatcaagacg cagacagaca aaggaaaagg caacgccgat
     7441 ttttgcaggg cttgtcaagt gtgcggattg tggttggtct atgagatttg gaacgaataa
     7501 gacaaataaa acgccttaca gttattatgc ttgcagttac tacgggcagt ttggcaaagg
     7561 taattgttct atgcactaca ttcgttatga tgtgctgtat caagccgtat tggaacgctt
     7621 gcagtattgg gctaaggcag tacagcagga cgaagaaaag gtattgaaca agatacagaa
     7681 agctggcaat gcagagagaa tacgggaaaa gaagaaaaag gcaagtacat tgaagaaagc
     7741 cgagaacaga caaaatgaga ttgaccgttt atttgcgaaa atgtatgaag atagagcctg
     7801 tgagaagata acagagcgaa attttgtgat gttgtccagc aagtaccaaa aagaacagat
     7861 agaactggaa cagcagataa caagcctaag agaagaacta agtaaaatgg aacaggatat
     7921 gataggtgct gaaaagtgga ttgagttaat caaggaatat tccgtaccaa aggaactgac
     7981 agcaccatta ttaaatgcca tgatagaaaa aatcctcata catgaagcaa caacgaatga
     8041 ggataacgaa agaatacagg aaattgagat atattaccga tttattggaa gagttgagta
     8101 atcaacaaga gtaatatttt taactaaggg aaaccggcgg tgtctatact tgggtcagtc
     8161 gcacattggg aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag
     8221 ttggctactt gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg
     8281 gtgctataca aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta
     8341 ttttactctc acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg
     8401 tagctggtat cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt
     8461 caggcgctcc actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat
     8521 tgcctacgct ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg
     8581 ccagtcatgc aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat
     8641 tggttattct ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac
     8701 ctcaacatgg tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac
     8761 acattgatcc aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg
     8821 tcatcggaga aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag
     8881 aagaaggatt acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt
     8941 tgatgatcgc aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg
     9001 gggggaataa tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt
     9061 tgtactttat gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa
     9121 gagcctatca ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt
     9181 tgacttcact ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac
     9241 agcatgggat ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat
     9301 ttacgatcta cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg
     9361 gttattcaaa tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat
     9421 caaacaggac aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta
     9481 ggtatggcga tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg
     9541 cgctttgcca tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac
     9601 ggaatagaaa aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct
     9661 atcatgaata tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga
     9721 gcgattgcaa caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc
     9781 ttgtcattta tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat
     9841 cattctgaat caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat
     9901 caaatgatcg aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc
     9961 aatgtcaccg ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct
    10021 ccttggaatg gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg
    10081 acaactgctg gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca
    10141 aaaagcggtg taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc
    10201 ttcagcccgg cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat
    10261 gtagtcaaag aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta
    10321 aaagtccaaa cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt
    10381 ttgtcccagt ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca
    10441 aaaatacgat ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca
    10501 ggaatgctgg aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa
    10561 ttatttgaat tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca
    10621 ccaacgatcc ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa
    10681 caagtaagag agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag
    10741 ctcacacagt ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat
    10801 ggtatattca cgagtacaga actggctgat cttgaattta tttctttgag taaagtatat
    10861 acaatcaaaa aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa
    10921 atcattatcc cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat
    10981 tgggaaggct atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat
    11041 tatgtagaga ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata
    11101 cgagaaatag agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat
    11161 aagaaaagcg cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa
    11221 aaaggaattc tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt
    11281 agaaattata ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa
    11341 aattttgttc tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct
    11401 gaaatcgacg atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg
    11461 ttgtgggcag atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa
    11521 caacttctgt acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc
    11581 caactaagat ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac
    11641 agtgaacaag cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa
    11701 caattttttt atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat
    11761 cctattttat ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga
    11821 gtggttttgt gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc
    11881 tatccaatct cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac
    11941 ctattaaaaa aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt
    12001 taattgtctg tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa
    12061 caagctttct ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag
    12121 aatagataaa gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg
    12181 tattatgagt acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa
    12241 attatctgtt gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa
    12301 ggacagcgat gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc
    12361 gtttcattat gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc
    12421 ttatggtcat aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga
    12481 agtgagcgga tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac
    12541 atctacatct atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc
    12601 aggaaatgtg gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg
    12661 attgaacaat ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat
    12721 gtctattgat gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa
    12781 cggtaaaagc agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa
    12841 tggaaacgat ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc
    12901 gattgtactt cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa
    12961 aatgaaatat cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac
    13021 ttctggagaa acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag
    13081 tacagctcct attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt
    13141 ccaagaaaaa catccagatc attattttga cgtggggatc gctgaacagc actctatcac
    13201 tttcgcttct gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt
    13261 cttgcaacgg gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat
    13321 tattttagtc ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga
    13381 cattccttac ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga
    13441 tatgatctct atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat
    13501 tcctacacat ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta
    13561 tcaaacagta aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt
    13621 aggaaaagaa acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc
    13681 aggaacgatc acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt
    13741 agtcatcaca ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta
    13801 ttatggtgat tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag
    13861 cgttcctgta gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga
    13921 tagtctgcgt gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta
    13981 tactagtatc tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag
    14041 caagtagaag aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa
    14101 ttaggtttct tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt
    14161 gtaaaaaaga taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag
    14221 cctttaccaa aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt
    14281 atcatcaaaa atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct
    14341 tggaaagaag gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt
    14401 tttaaacatg cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta
    14461 tacgaagaat ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca
    14521 ttattgtctc ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac
    14581 attactggag gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct
    14641 tgtcaagctt tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg
    14701 gctgcaaaag aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa
    14761 atcaagcgaa tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca
    14821 gaagtgttta ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa
    14881 atcattgcac tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt
    14941 gtccaaatga ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa
    15001 gcgcttcaag cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca
    15061 gaatattcac gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa
    15121 gtcgatatcc cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca
    15181 tatgaattag gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc
    15241 actaaaagat atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag
    15301 attcttgaca aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa
    15361 aagcattact tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag
    15421 gcgaaaccat gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc
    15481 tgattggttc agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata
    15541 tagtgaatat cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat
    15601 ccttggcgat ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga
    15661 caagagaagc tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag
    15721 cagccaatct gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt
    15781 cagtaatcat ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat
    15841 atgaacatgt cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag
    15901 tacagaaata tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa
    15961 gaaaattgaa tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt
    16021 tcacgattgc acgtcccgaa taaaaaggag cactagttat gttaaaaata agctataaac
    16081 catcgacaga ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag
    16141 ggcagtactt agaagttccg ccattgcaag accattttgt cgttactact gttactttag
    16201 atggtaagga aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca
    16261 aataaatatt tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat
    16321 tatcaaagaa tcctggtgca act
//
LOCUS       DAEGIQ010000320        11844 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.084, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000320 DAEGIQ010000000
VERSION     DAEGIQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.084"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            14..238
                     /locus_tag="KMT45_003110"
     CDS             14..238
                     /locus_tag="KMT45_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE7006468.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            355..852
                     /locus_tag="KMT45_003111"
     CDS             355..852
                     /locus_tag="KMT45_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBE7006469.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            939..1331
                     /locus_tag="KMT45_003112"
     CDS             939..1331
                     /locus_tag="KMT45_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBE7006470.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1315..3762
                     /locus_tag="KMT45_003113"
     CDS             1315..3762
                     /locus_tag="KMT45_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE7006471.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3765..5948
                     /locus_tag="KMT45_003114"
     CDS             3765..5948
                     /locus_tag="KMT45_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBE7006472.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5945..6946
                     /locus_tag="KMT45_003115"
     CDS             5945..6946
                     /locus_tag="KMT45_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBE7006473.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6961..7875
                     /locus_tag="KMT45_003116"
     CDS             6961..7875
                     /locus_tag="KMT45_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBE7006474.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8120..8236
                     /locus_tag="KMT45_003117"
     CDS             8120..8236
                     /locus_tag="KMT45_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBE7006475.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8252..10171
                     /gene="tet(M)"
                     /locus_tag="KMT45_003118"
     CDS             8252..10171
                     /gene="tet(M)"
                     /locus_tag="KMT45_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBE7006476.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10269..11642
                     /gene="tet(L)"
                     /locus_tag="KMT45_003119"
     CDS             10269..11642
                     /gene="tet(L)"
                     /locus_tag="KMT45_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HBE7006477.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         3711 a         2119 c         2481 g         3533 t
ORIGIN      
        1 tgagatattt ataatatcag gaacttacct atccatatat aactggtttc tatcaaacgc
       61 tcaatcactg gtgcttttag caatcgttgt gattggcttg tatcttggct tcaagcgtga
      121 gtttagcaaa ctgattggct ttttaattat tgcgattatt gcggttggct tagtcttcaa
      181 cgctgctgga gtaaaagaca ttttactaga gctattcaat cgcattattg gtgcttaaat
      241 aaaaccgttc ttttgtggaa tataagtggt tttcttatgt tccgcaaagg aatggtacac
      301 caaacgaagt gcggtaggga tttttgaatc tctacaaaga aaggacgtga atatatggac
      361 gatatgcaag tctatattgc gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg
      421 tggtttacct ttcccattga ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa
      481 tatgaggaat acgccattca tgattacgag ttacccttta cggttgacga atacacttct
      541 attagcgaac tcaatcgcct atgggaaatg gtatcggagt tgccagaaga actacaatcg
      601 gagctatctg ctctgctcac tcatttttca agtattgaag aactaagcga acatcaagaa
      661 gatattatca ttcattccga ttgtgatgat atgtctgacg tggcacgcta ctacattgaa
      721 gaaacgggtg ctttaggcga agtgccagcc agtcttcaaa actatattga ttatgaatcc
      781 tatggtcggg atttagacct ttcgggaacg tttatctcaa ccaatcacgg gatttttgaa
      841 atcgtctatt aaatctgtcg gtacattctt actggcagat tttcttgttt acggggtagt
      901 caaaagacta tccccatttt tatgaaagga ttgattacat gaagaaaata cgaagctata
      961 ccagtatctg gtctgttgag aaagtgttgt actccatcaa tgactttaga cttccgtttc
     1021 ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtt atgatacttg
     1081 gcaacttgcc ccctctttct atgatagagg gagcatttct caaatacttt gggattcctg
     1141 tggctttcac atggtttatg tctacaaaaa cctttgatgg taaaaagcct tatggatttt
     1201 tgaagtctgt cattgcttat gcactgcgac gaaagctgac ctatgcagga aaaaaagtaa
     1261 cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa ttttatggca
     1321 tatccaatta aatatattga aaacaatctg gtctggaata aagatgggga atgttacgct
     1381 tactatgagc ttgttcccta caattactca tttctaagtc cggaacagaa aatacaagta
     1441 catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaagattca tgctttacaa
     1501 atcagtacag aatccagcat actttctgca caagaacgtt ccaaaaatga agtcactgga
     1561 aagctcaaaa cggttgccta tgacaaaatc gaccaacaga cagacgcttt aatatccatg
     1621 attggcgaaa atcaagtgga ctaccgtttc tttatcggtt ttaagttgct tctcaacgat
     1681 caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgcttttac tgattttgtc
     1741 tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga tgaaatcctg
     1801 cgttttcaga agatggaaaa gcttttagaa aataaaatct cccgtcgttt caaaatccga
     1861 agattagata aggatgactt cggctatctg attgaacacc tttacggaca gacaggcact
     1921 gcctatgaag agtatgagta tcctctatca aagaaaaagc tggaacatga aacactgatt
     1981 aaaacctatg acctcattaa gcctactcgc tgtctggtgg aagaaaaaca gcgatatttg
     2041 aaaatccagc aggaagacga aaccgcctat gtagcttact ttaccatcaa cagtattgtc
     2101 ggcgaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca atttaccttt
     2161 ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct atctactgtc
     2221 cgcaataaaa agaaagaact gaaagacttg gataaccacg cttggcaaag tgataatgaa
     2281 accagctcta atgtggcgga agctctggaa agtgtgaacg agctggaaac caatttagac
     2341 caaagcaagg aatctatgta taagctgtcc tatgttgtaa gggtatcagc aaatgacctt
     2401 gacgaactta aacgtcgttg taatgaagtg aaagatttct atgatgattt gagcgtgaaa
     2461 ctggtacgac cttttgggga tatgctgggc ttacatgaag aatttttacc tgccagcaaa
     2521 agatatatga atgactatat tcaatacgtg acctctgatt tcctcgctgg tttaggtttt
     2581 ggtgctactc aaatgctggg tgaaaatgag gggatttatg ttggctacag cttagatact
     2641 ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg ttcagtaacc
     2701 aatgcgttag cgtctgcctt tgtcggttcg ctgggtggtg gtaaatcctt tgcgaataac
     2761 cttatcgtct attatgcagt gctttatggg gcacaagcag tgattgtaga cccaaaagca
     2821 gaacgtggca gatggaaaga aaccttgcca gagatttccc atgaaatcaa tatcgtcaat
     2881 ctgacttctg atgagaaaaa caaaggctta cttgaccctt atgtaattat gaaaaatacc
     2941 aaagattctg aatcactggc tattgatatt ttgacattcc ttacggggat ttcctctcgt
     3001 gatggggaac gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa tagtgaagtg
     3061 cgagggttga tgaaagtgat tgaagaatta cgggttgaga atacgccact aagtaccagc
     3121 atagccgacc atatcgaaag ttttacagac tatgactttg cccatctgct ttttagtgat
     3181 ggttatgtag agcagtctat cagccttgaa aaacaactga acattataca ggttgccgac
     3241 ttggtacttc ctgacaagga aacttccttt gaggaatata ccacaatgga gttactatcg
     3301 gtagcaatgc tgattgtcat tagtaccttt gcgttggact ttatccatac agaccgaagt
     3361 attttcaaga ttgtagactt agacgaagca tggagcttct tacaggtggc acaagggaaa
     3421 acactatcta tgaagctggt tcgtgctggt cgtgctatga acgctggggt atatttcgtg
     3481 acccaaaata cagatgacct cttagatgaa aaactgaaaa ataacctcgg cttaaaattt
     3541 gcctttcgtt ccactgacct taacgagatt aaaaaaaccc ttgccttttt tggtgtagac
     3601 ccagaggacg aaaacaatca gaagcgattg cgtgatttgg aaaacgggca atgccttatc
     3661 agtgatttat atggtcgtgt cggtgtgata cagttccacc ctgtatttga agaactgctc
     3721 catgcctttg ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa ccatcaatat
     3781 taaacagaat aaaatcaaac tggacgctga aacgtctagg taaagtggca atgacagtgg
     3841 ctttcacact tgtgattgcc atttttcttt tagccatgct gggaacggtg gttcaagctg
     3901 cgggcttggt agatgatacg gtcaatgtgg caaatgaata cagccgatac ccacttgaaa
     3961 actatcaact ggatttttat gtggataata gctggggctg gctaccgtgg aactggtcgg
     4021 acggaattgg aaaacaagtc atgtatggac tatatgccat taccaatttt atttggacaa
     4081 tcagtcttta tgtttccaat gcgacgggtt acttagtaca ggaagcctat tccttagact
     4141 tcatttccgc tacagcagat tccattggca agaatatgca gacccttgca ggggttacgc
     4201 ctagtggact atcaacagag ggtttctatg ttggattcct tttactcttg attttggttc
     4261 ttggggttta tgttgcctat acaggactga taaagagaga aaccacaaag gcaattcatg
     4321 ccctcatgaa ttttgtgctg gtttttatcc tatcggcttc ctttattgcc tacgctcccg
     4381 actacattaa aaaaatcaat gacttttcat cagacatcag caacgctagt ttgtcactgg
     4441 gtacaaaaat tgtcatgccc cattcagata gtcaaggcaa ggacagtgtg gacttgatac
     4501 gtgacagcct attttccata caggttcagc aaccgtggct actgcttcaa tataacagtt
     4561 cagacattga aagtattggt attgaccgtg tggaaagcct gctctcaacc agtccagatt
     4621 ccaacaatgg ggaagacaga gaaaaaattg ttgcggaaga aattgaagat agaagcaata
     4681 ccaatatgac gattacaaag acgattaacc gtttaggtac agtcttcttc ctatttgtct
     4741 tcaatattgg gatttccata tttgtattcc tattaacagg aatcatgatt ttctcgcagg
     4801 tactttttat catctatgct atgtttctgc ctgtgagctt tattttaagc atgattccat
     4861 catttgatgg tatgtcaaaa cgagccataa caaagctctt taataccatt ttgacacgag
     4921 ctggaatcac attgattatt acgacagcat ttagtatttc aaccatgctc tataccttat
     4981 cggctggtta tccgttcttt ttgattgctt ttctacagat tgtgaccttt gcaggaatct
     5041 actttaagct gggcgattta atgagtatgt tttctctaca gagtaacgat tctcaaagtg
     5101 tgggaagtcg tatgatgaga aaacctcgta tgcttatgca cgctcacatg caccgtctac
     5161 agcggaaact tggacgctcc gtgactgctt taggggctgg gtctgccatt gccagtgcta
     5221 cgggtaaaaa aggacagtcg ggttcgggaa gttctgcgag tacacaagca gatcactctc
     5281 gaccaaacgg gcaggaaaaa tcaacactag gaaagcgtat cggtcaaacc atcggtacag
     5341 tagctgatac caaagacaga atggtagata ctgctggtaa tttgaaagaa caggttaagg
     5401 atttgccgac caatgcaaga tatgcagtat atcaaggaaa atctaaagtg acggataatg
     5461 tccatgattt aaccagaagt atttctcaaa ccaaagcgga taaagccagt ggacgcaaga
     5521 aacagcagga acaaagacgg aaaaccattg cggagcgtcg ctctgaaatg gaacaggtca
     5581 aacagaaaaa acagccagct tcttccgttc atgaaagacc agccactaag caagaacaat
     5641 ctcatgatgg acagacttca aggcaaacaa ctgtacaggc ttcctatagg gaatctcaac
     5701 aagccaaaca agagcgtcca acagttaagt ccgattcttc aagtttaaaa acggaacgtc
     5761 aaagtaatac agttcaagaa agaacggttc aaaagccagt aacttcaact gcaccaacag
     5821 atagagcttc acaacgttca atcacaaaag aacgtcagtc tactgttcaa agagtaccac
     5881 tacaaaatac aaaaagtaga ccaccaatca aaaccgccac cattaagaaa ggcagtaaga
     5941 aaccatgaag ctgaaaactt tagtgattgg tggttcggga ttattcttga tggtcttctc
     6001 actgcttctg tttgtcgcca ttttattttc agatgaacaa gatggcggtt tttccaacat
     6061 tcactatggc ggtgtggatg tttccgcaga agtgctggct cataagccta tggtagaaaa
     6121 agttgccaaa gaatatggaa ttgaagaata cgtcaatata cttcttgcga ttatacaggt
     6181 ggaatcgggt ggtacagcag aagatgttat gcagtcctcg gaatccctcg gtcttccacc
     6241 taattcattg agtacagaag aatccattaa gcaaggtgtg aagtatttca gtgagttatt
     6301 agccagtagc gaaaggcttg gtgtagattt agaatcggtt atccagtcct acaattatgg
     6361 tggtggtttc ttaggatatg tggctaatcg tgggaataaa tatacctttg aactggctca
     6421 aagtttctca aaagagtatt cgggtgacga aaaagtgtct taccccaatc ccatagccat
     6481 acctatcaat gggggctggc gatacaatta cgggaatatg ttttatgtgc aactggtaac
     6541 gcagtatctt gtcacaacag agtttgatga tgatacagta caagccatca tggacgaagc
     6601 tctgaaattt gaaggctgga agtatgtcta tggcggagct tccccgacta cctcttttga
     6661 ttgtagcgga ctgacacaat ggacgtatgg aaaagctgga atcaacttac cacgaacagc
     6721 acaacagcaa tatgatgtaa cccggcatat cccattattg gaagcaaaag ccggcgattt
     6781 ggttttcttt cattctacct ataacgctgg ctcttatatt actcatatcg ggatttattt
     6841 aggcgataac cgtatgtttc atgcaggcga ccctatcggt tatgccgact taacaagctc
     6901 ttactggcaa cagcatttag tgggagcagg acgaatcaaa caataagaaa ggacgattca
     6961 atgatgaaat tcggaaaaaa tcagaataca gaaaaacaga caccaaagga aaagaaacct
     7021 cgtgtctata aggtcaatcc tcgtaaaaag gttgtgattg tcctatgggt acttttagga
     7081 cttagtttca gctttgcgat attcaagcac tttacagcca ttgataccca taccattcat
     7141 gaaaccacca tcatagaaaa ggaatatgtc gatactcatc atgtggaaaa ttttgtagag
     7201 aactttgcga aagtctacta ttcatgggaa ctaaacgaca agtccattga taatcgcata
     7261 gaaaacctga aagcatatct gacagaggaa cttcaagctc tgaatgtcga tacagttcgt
     7321 aaagatattc ctgtatcgtc ttctgtaaaa ggatttcaga tatggacggt agaagcagat
     7381 ggcgacaatc aatttgatgt aacctacagt gtagaccaac tcattacaga gggggaaaat
     7441 acaaagaccg tccactctgc ttatatagtg agtgtctatg tagacagtac tggaaatatg
     7501 gtcctcatta agaatccgac cattactagc ataccaaaga aatcagacta taaaccaaaa
     7561 gctattgaaa gtgagggtac ggttgattcc attacaacca atgaaatcaa tgagttttta
     7621 acgacgttct tcaagctcta tcctacagcg acagccagtg aactttccta ctatgtgaat
     7681 gacgggatat taaaaccaat cggaaaagag tacatctttc aagaactggt aaatcctatt
     7741 tacaatcgta aggataatca agtcacggta tcgctgacag tggagtatat cgaccagcag
     7801 accaaagcaa cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg gagtaattgg
     7861 aagattatag aataacaaat attggtacat tattacagct attttgtaat cacgtactct
     7921 ctttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagta
     7981 tctatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
     8041 atagtgggaa caaataattg gatgtccttt tttaggaggg cttagttttt tgcacccagt
     8101 ttaagaatac ctttatcatg tgattctaaa gtatccggag aatatctgta tgctttgtat
     8161 gcctatggtt atgcataaaa atcccagtga taagagtatt tatcactggg atttttatgc
     8221 ccttttgggc ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
     8281 tcatgttgat gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat
     8341 tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg
     8401 tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt
     8461 gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc
     8521 agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg
     8581 tatattattt catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat
     8641 tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc
     8701 cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac
     8761 cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat
     8821 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
     8881 ttgctccttg taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct
     8941 tatagaagtg ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg
     9001 cggaaatgtc ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct
     9061 ttatggcgga atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa
     9121 aattacagaa atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc
     9181 cggggaaatt gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac
     9241 aaagctattg ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt
     9301 tgaaccgagc aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga
     9361 cagtgacccg cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt
     9421 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
     9481 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
     9541 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
     9601 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
     9661 tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata
     9721 tggttggaat gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt
     9781 tagtacccca gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa
     9841 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
     9901 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
     9961 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
    10021 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
    10081 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
    10141 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
    10201 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
    10261 aattaattat gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc
    10321 tttgcgtttt atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg
    10381 atattgcaaa tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta
    10441 tgttaacctt ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca
    10501 aaaggttact cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg
    10561 gccattcttt cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg
    10621 catttccagc actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta
    10681 aagcatttgg tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg
    10741 gtggaatgat agcccattat attcattggt cctatcttct actcattcct atgataacaa
    10801 ttatcactgt tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt
    10861 ttgatatcaa aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa
    10921 catcatatag catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac
    10981 atatcaggaa agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta
    11041 tgattggagt tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg
    11101 ttccttatat gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta
    11161 ttttccctgg aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata
    11221 gaagaggtcc tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa
    11281 ctgcttcctt tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt
    11341 taggtgggct ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac
    11401 agcaggaagc tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa
    11461 caggtattgc aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac
    11521 ctatggaagt tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa
    11581 tcattgtcat tagttggctg gttaccttga atgtatataa acattctcaa agggatttct
    11641 aaatcgttaa gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg
    11701 aattcaaatc tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc
    11761 tctcttgtat cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca
    11821 ataaaaattt tattcttgct gagt
//
LOCUS       DAEGIQ010000321         8061 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.114, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000321 DAEGIQ010000000
VERSION     DAEGIQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.114"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            128..1564
                     /locus_tag="KMT45_003120"
     CDS             128..1564
                     /locus_tag="KMT45_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBE7006478.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="KMT45_003121"
     CDS             1753..4200
                     /locus_tag="KMT45_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBE7006479.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="KMT45_003122"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="KMT45_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBE7006480.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="KMT45_003123"
     CDS             complement(5690..6793)
                     /locus_tag="KMT45_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBE7006481.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="KMT45_003124"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="KMT45_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBE7006482.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2695 a         1375 c         1751 g         2240 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DAEGIQ010000322         7131 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.127, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000322 DAEGIQ010000000
VERSION     DAEGIQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.127"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            48..1163
                     /locus_tag="KMT45_003125"
     CDS             48..1163
                     /locus_tag="KMT45_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBE7006483.1"
                     /translation="MQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEI
                     TNQLTDGQTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDL
                     YASVMIAQAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAA
                     FRKYPSYKESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNY
                     GSTLNNVIETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEG
                     IAWQAPNSGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRL
                     FNPNEKTGTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            1650..2114
                     /locus_tag="KMT45_003126"
     CDS             1650..2114
                     /locus_tag="KMT45_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE7006484.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2098..2727
                     /locus_tag="KMT45_003127"
     CDS             2098..2727
                     /locus_tag="KMT45_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBE7006485.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            3081..4559
                     /gene="msr(C)"
                     /locus_tag="KMT45_003128"
     CDS             3081..4559
                     /gene="msr(C)"
                     /locus_tag="KMT45_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBE7006486.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            4606..5052
                     /locus_tag="KMT45_003129"
     CDS             4606..5052
                     /locus_tag="KMT45_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBE7006487.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5251..5766)
                     /locus_tag="KMT45_003130"
     CDS             complement(5251..5766)
                     /locus_tag="KMT45_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBE7006488.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            5883..7085
                     /locus_tag="KMT45_003131"
     CDS             5883..7085
                     /locus_tag="KMT45_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE7006489.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         2243 a         1233 c         1488 g         2167 t
ORIGIN      
        1 catcgagttc ttcagaaaca ggaattgaaa acgatacaga aagtactttg cagtcagcta
       61 ctgtctcaga aaatacaatt tcaaccgaag aaccaacaac tgaaacaaca gaacaaacac
      121 aaaccgaaga cagtacatca gcagaagctc agaacaatcc agagaatgca gaagaaatca
      181 cgaaccaatt gacagatggg caaacattga cacataccca tcaagcagat ttgtctgaat
      241 tcgaaaacta tactggctat gctttttatg ctcgctcatc tgctaattct caacaagcgt
      301 tcattgatag tattgcctca actgcacaaa gtttggcaag tgccaatgac ttatatgcat
      361 cagtcatgat tgctcaagcc atcgttgaaa gtggctgggg gaacagtact ttggcttctg
      421 cccctaatta caatctattt gggattaaag gaagctataa tggccaatct gttacgatgc
      481 ctacttcgga atatgtgaat gggcaatgga ttacggtgaa tgccgctttt agaaaatatc
      541 cttcgtacaa agaatcattg caagataacg taacggtatt gaagacgact tcttttcagc
      601 caggcgttta ctactattcc ggtgcatgga aaagcaatac gaactcttac aaagatgcta
      661 cggcttggct aacaggtcgt tatgccactg cgcctaatta tggttctaca ttgaacaatg
      721 taatcgaaac ctataatctg acacagtatg atacagcacc aacttcaact tctccgatgt
      781 atcgtctgta taaccgtcat acaggagaac atttatatac attgaatgcc ggagaaaaaa
      841 attatctgcc aactgtaggc tgggaatatg aagggattgc ttggcaagca ccaaacagcg
      901 gacaacctgt ctaccgttta tacaatcctt acagtggaga ccatcattac acaatggcac
      961 aatctgagat caactcttta acaaaaatcg gttggcgtta tgaaggtcta agtttttatt
     1021 ctggtggttc caagccaatt tatcgactat ttaatccaaa tgaaaaaact ggtacccatc
     1081 actatacctt gagtgctaag gaaagagatt ggctaacacc gatgggctgg agatatgaag
     1141 gaataggatt ttatggttat taaaaagagg tagtgaaaaa gctgccctgc atcaagtagt
     1201 aagaacagcc aaacaaaaac agattttcat gttttttgtt tggttgaact tattaccgca
     1261 gatgcaagtt tttgaacacc gtttactcaa aaaaggttgt gacaacatct tgtcataact
     1321 tttttattac tgtttaagca tactgatcca atttctaata ttcgacggga ttcttgcaaa
     1381 aggcgatttc ggaaattttt ggcggtgtaa aattatctac tgcagaagag ctatcttcta
     1441 tagtttgata agaatcaata atcttggttt tagcgttgtg ctttctttat cattttttcc
     1501 gttcattcta caaaaaattt cttttctcta tacttttcta ttgagagcgt tttttttatt
     1561 acacagctga ttctttgact tttttctttt tttattctat tattgacaag tcaatcgttg
     1621 acgagtcaat tattagaaag gaggaaatta tgtctttaga agagattagt caccttttat
     1681 atcaaatcaa attagccgat caaaaaatcg tccagatctt tgaagaaaat atcggtataa
     1741 gtcttactag atacgagatc atgatgagac tgaaagaaga aaatccattg ctacaaagca
     1801 gtttgcaaga aaagttgcag attgatcaag gagcagttac tcgtcatctg aaggtattgg
     1861 aagcaaatgg ctatgtttcg cgtcagagaa atcctcagaa caaccgggaa gtgtatgtag
     1921 cgctaacaga aaaagcccag aagaaaatca agcattgtga aatggaccat aaagatacag
     1981 gttccgtgct atccccggct ttcagcgatg tagaaatgaa acaatttcta acacttttag
     2041 ataaattgaa tacccaactt tgcaatataa caacagaaac gaaagaggtt aataataatg
     2101 gaacaaaaaa atgatttttc aacaattatg actggtagaa gatccgttcg cgtatatgat
     2161 gaaacataca aaatcccaca cgaagaaatg ctggaaatga tcaaagaagc gacgactgca
     2221 ccttcttctg tcaatatgaa accatggcgc tttgtggtcg ctgaaagcga ggaagcaaaa
     2281 gaaatcttgc gtcctttgat ccgtttcaat acgagacaaa atgatacttc ttctgcaatg
     2341 gtcatgattt ttggcgatat gcaatgttac gaatatggcg aagaaatcta caaccaagct
     2401 tacgaatcag ggaagatgcc aaaagaagtc agagatcaac aattagctgc gatcattcct
     2461 tattacaaga gtttttcaag agaagaaatg aacgatgtag tcaaagtcga ttctagctta
     2521 gcggctatgc aattcatgtt agttgcgcgt gatcatggct atgaaacaaa tccaattggt
     2581 ggattcgaag cagatcagtt ggcagaggca tttggtttgg ataaagaccg ttatgttcca
     2641 gttattattt tatcaatagg aaaagcggta gaagaaggtt acgagtctgt tcgtttagca
     2701 ccagaaaaga tcactatgtt cgaataaaag gttttcagtt actattcagt agctattgct
     2761 ttttttgaat cgtgtgtggt aacctaataa aaaaggaggt cggaaaaatg actgcatcga
     2821 tgaaattacg tttcgaactt ttgaataaca actaaataaa ttcaaaagac atgtataaac
     2881 aacaatttca tggatggagt atgtccattt tttcgaacga cagtagaata ggtgaaagct
     2941 atgacagatt tggtgattta gtcatagtta tttcgttcag agtaggaaaa gatgtgatgc
     3001 agtttttttg caaacagatt tttctgctct tttttagtat cgataattgt tgttttacat
     3061 gaaataggag tgtaaaacat atggaaaatt tagcagtaaa tataacaaat ctacaagtca
     3121 gctttggcaa ccagctagaa ctatctattg attctcttcg tgtctatcag caagatcgta
     3181 tagggatcat aggagaaaat ggtgtcggta aatcgacttt gctcaaacta atagccggtg
     3241 aactttttcc cgatcatggg aaaatccaaa cagagatcac cttcaactac ctgcctcaat
     3301 taacctatct cgctgaagca aaggacctaa atttggaatt agccagtcat ttccagttaa
     3361 gactggaaga aacttcagag cggaaatgga gcggagggga agaacgaaag atcgagttga
     3421 tacgtcttct ttcttcttat gaacaaggga tgcttctaga cgagccaaca acccatctag
     3481 atagaaaaag tattgatcga ctgattgaag agcttcgtta ttattatggt acgctggttt
     3541 ttgttagtca tgatcgctat tttctagatg agctagcatc gaaaatctgg gaagtaaaag
     3601 acggagaaat ccgagagttt tcggggaatt atagtgccta tctcactcaa aaggaattgg
     3661 agaaaaagac tcagctacga gaagcagagt cgatcatgaa agagaaaaaa cgattggaaa
     3721 aatcgatcca agaaaagaaa aaacaagcgg aaaagttgga aaaagtgtcc agtaaaaaga
     3781 aaaagcaaca aatcagactg gatcggttgt cttcctctaa acaaaaagac agtgtacaaa
     3841 aagccatcca aaagaatgcg aaaacattag agagaagact ccaaaaaata ggagaaacaa
     3901 ccaagccgca gcagatgaaa caaatccgtt ttccagtacc aaaatctctt gaactccaca
     3961 gccgctatcc catcatggga caaaatgtcc aattggaaag aagtgggaga acattactgg
     4021 taaatggtga ttttcagttt tctttaggta aaaaaatcgc gattgtcggt gaaaatggtt
     4081 caggtaagac aaccttatta gaacatatcc gcaaacaagg agaaggaatc cttctctctc
     4141 cgaaagtaag ctttcaagta tatcagcaaa agggttatca aatgacatct gaagaatcca
     4201 tcattcgttt tgtcatgaga caaacagagt tttcagaatc gcttgtccgt agtttgctga
     4261 atcacttagg gtttgctcag gaaactttga cgaaaccgtt atgtacatta agtgggggag
     4321 aagcgacccg tctgacgatt gctttgcttt ttactaagcc aagtaatgtg ttgctgttag
     4381 atgaaccgac taattttatt gatatggcaa cgatcgaagc tttagagaag ctaatgcaaa
     4441 tatatccggg aacgattttg tttacttctc atgattctta ctttgtcgag cgtacggctg
     4501 atgaagttta tgaaataaaa gggcagaaaa taaaaaaagt acttacgaga aatttttaaa
     4561 tgtgctacga tgattttcgt gacaaattta aaggagtggt acagattgcc tacaaataaa
     4621 aaagaaggaa taatttttac aacaatgatg tgttttttga tggtcttggg gatgagcgcg
     4681 tataatttat ggctgcatca agatcttcat ttatcaagtt tgttgattgg tctagtgcct
     4741 ggattcgtgg tggctttcat tttggatgtc ttcatcatcg gtgtgctggc gaaaaaaatt
     4801 gcctttaaac taccagtaaa tccagaaagc aaggtccaaa tgatcttaac tatttcatgc
     4861 ttgatggttt taggaatggt gacatgtatg tctttattcg gtgtgatcat ggaacaagga
     4921 atacctgatc aattattctc tgcttatatg aatgcttgga aaatgaatgt gattgccgct
     4981 ttaccgttgc agttgatcat cgttggcccg ctttcaagaa gtgtgctaaa agcaattcaa
     5041 agtaatgaat gatggaacac tgttatttga aaaaaggcct tggcatgctt tttgtcatag
     5101 tctcttgaat gcagttctag aaaaatagtg atccgaacga taggttttgg cctgtcgttc
     5161 ggatcgcttt ttcttttcca actgtattgc tcttttattg aagataattt gctcttcaat
     5221 agatatttct agaaaggttc tgtttatccc ttactcaaaa aactgctgca tttgttctct
     5281 tacttgctga caccattgga gttcctgctg acaaagcata attctgcgct ggatcacaac
     5341 ataacgtcca aattcttgtt tcttgtctag cgagaggtca ttcaattttt tttgattttt
     5401 ttgcatgatt ttgttcagct gttgttctct agtgaatagc agtccagtag ctgttgtttt
     5461 atcgagcgtc gaaaaagcat agatttttgc tagaaattca tctttttgcg taggagcggg
     5521 agtttcctct ttgatccatt cctctacaag agcacgtcct tcatcagtca agtggtagat
     5581 ctttttttga gagtgctcgt ctttgtccaa aacggtcaca taaccctgct ggtctaactt
     5641 ggcaagcgtt gtatagatct gactgtgatg ggcttcccaa aaaattgtga tcagctgctt
     5701 caattcatac ccagaacgtg gtttggataa aagcacgcat aataatatat aggctaaagt
     5761 attcataaaa atcctacctc tcgtcttatt ttctttatta tatcacagta aagtttccac
     5821 gttttacatg tgcatattga catgtaaaat aaatcatgta tgatgctaat agagagaaag
     5881 ggatggaaat attggagaat caaatagata tacaagaaca aacaaatgaa gaaacaaagg
     5941 ggaaaagttc agcgctagtt gtagcagcta cctgtatgat cgcttttatg ggagtgggtc
     6001 tagtagaccc aatcttaaaa acgattgctg cacagttaaa cgctacgcca gctgaaacga
     6061 ccctgttatt tactagttac atgttagtca ctggtgtggt gatgctattt acaggtttct
     6121 tatctagtcg gatcggtttg aaaaaaacat tgatgacagg attgtttatc atcgttgtat
     6181 tcgctggtct tggcggatta tctgggaata tcggaatgct gattgggtta cgtgccggct
     6241 ggggtgtcgg gaatgcttta tttgtctcta ctgctttagc aacgatcgtc agtattttag
     6301 ttggtaacac agaaaaagca atcatgatgt atgaagcagc acttggctgc ggaatggctg
     6361 ttggccctct agtcggtggg gtcttgggca gtggttcatg gcgttatcca ttttttgggg
     6421 tagcattttt gatgttttgc ggttttatag ccctagcggt acttttacct gaaactgaga
     6481 aaccaaagag aaaagtaggc ctttgggaag gtgtcaaagc gttaagcaat cataaacttc
     6541 gttcagttgg actaatcgca ttgttatata atttcggatt ctttacttta cttgcgtatg
     6601 cgccattttt attgattggc tattctgaat tagaagtcgg ttttgtattt tttggctggg
     6661 gtgtgcttct ggctattgct tcgattttcg ttgcaccggc tcttgagcgg aagtttacga
     6721 cttatcggac aattttagca gcatttgtct tattcattat ttgtttgctg ttattaggag
     6781 ctggaacttc tatgccagtc cttgtaccga tcagcattgt gttagcagga tttttccaag
     6841 gaattatcaa tacactgttg acgacgattg ctatggaaat accaggtctg caacgaaacg
     6901 ttgcttcgtc cagctatagt tttgtccgct ttttcggtgg agctttagca ccatttattg
     6961 caggtgtgat tggtgaacat attggtgaaa gctatgcgtt ttattttgca ggtgccattg
     7021 ttcttatcag tgtatgttgt atcatttatc accgtaatta ttttgctaca aatgaagaag
     7081 aataagaaat cagaggttgt gaaaaagctg tcctgcatca agtcataaga a
//
LOCUS       DAEGIQ010000323         5906 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.140, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000323 DAEGIQ010000000
VERSION     DAEGIQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.140"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            177..1073
                     /locus_tag="KMT45_003132"
     CDS             177..1073
                     /locus_tag="KMT45_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBE7006490.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(1216..2157)
                     /locus_tag="KMT45_003133"
     CDS             complement(1216..2157)
                     /locus_tag="KMT45_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBE7006491.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2283..3404
                     /locus_tag="KMT45_003134"
     CDS             2283..3404
                     /locus_tag="KMT45_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBE7006492.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3662..5164
                     /gene="eat(A)"
                     /locus_tag="KMT45_003135"
     CDS             3662..5164
                     /gene="eat(A)"
                     /locus_tag="KMT45_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBE7006493.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5192..5758
                     /locus_tag="KMT45_003136"
     CDS             5192..5758
                     /locus_tag="KMT45_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBE7006494.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
BASE COUNT         1936 a         1037 c         1239 g         1694 t
ORIGIN      
        1 aattacaact attcagaaaa cttttgggca tattttaatg gaagaatgat ttatttcatc
       61 gtattttcat agaaatgtta aatttttata aaaataatag gaagcgtttt actggctttt
      121 tactatttaa acaggctatc ataggaaaaa gtgacagaga aagtgaggcg gaacagatga
      181 acaaaaacga cgtattgcta attgcgaaaa ataaatcact ttattttttt ccttccatcc
      241 gctctttaga tgtttggtcc ggaaaagatt tactacagtc ggaattcatt aaatcagctc
      301 gacaacagtt ggcaaattat cagctgatcg tttttttaga ttatggattt gatatggaga
      361 tggctcgacg cgtgcgtcca tttaccaatg caaaaatcgt gttatttttt tggaaccact
      421 ttaaagcaga acacgaggcg atgctgaagc aggcacaaga tgaaccggca atcgatgaga
      481 tttatcattt tgatttttta gaagcgaaag aacttggact aaaacataat agttcctttt
      541 attcgaaaca tatggatttt tcttctagcg aaccaaagac ggatctattt tttggtgcaa
      601 caaataacgg cagaaaagaa cgggcagaaa aatataagca ggaatttaca aaaagaggtg
      661 ttacgaccaa ttactttatt cttccgcttc gaggaaatga acaacctgga taccttactt
      721 atgaagaata tctgaaaaaa accggggaat cgagggggat tctagaactg ttgcgggaag
      781 ggcagcaagg agtgactttg cgaacgtttg aatctatgta ttttcaaaag aaactagtga
      841 cagataatca agccatagct cattatcagt tttatcattc tgaaaatatt tttttgcttc
      901 aagaacgtga tttagatgaa ttaccggaat ttttgcatac gccatttcaa ccaatcccat
      961 cagaactgtt ggaatttttt gatgcagaaa attgggcaaa acgttttttc actactgact
     1021 ccgctattta tgaacaatat gaatacgatc tttcctctgt taaaaataaa taaaagtgcc
     1081 aaccaatcat agatgaaaat aaacaagcgc aattcttatg gcaacatgag aattgtgttt
     1141 ttctttttga actttcatgg gggtaaaaaa atgagaacca gctgctgatc cagctgattc
     1201 tcatcagaga gccttttatt ctgcgccaag gattccttct tttgcatgga ctaattgggt
     1261 caaaaaggca caatatggtg tagcaatatc atatttttgt ccttttcgcc aaacggcacc
     1321 attgatgtag tcgatttcag tcaaacgatg gtttttgatc aggtcttggt acatagatgg
     1381 gtaatgcaac ccgatgttgt caggatcata agtagaagca atatgttggt acacttcttc
     1441 ttgatctaat gtgattcctt cttttgctgc gatagaagca aactcagata caatcgttcg
     1501 aaccatgccc tctgccgctt tttgcgcacc taattcagca atattacaat ccaatatcgt
     1561 gcataatcca tttaatgttc cgtttacaca agcttttcgc caaatggaat aacgaacatt
     1621 atgactatag attggattca attttgcttc ttgaaagact tctacaactt tttttgtaaa
     1681 tgcttcgcct tctggttcca agttctctag ttcgatttct ccatcaccta ataatgtgac
     1741 ttttccggga ccagctagac cagctgtcca catcgtgatc ccaaataaga tatttttctt
     1801 tggtacatat ttttccagta catcttcatg acctaatcca ttcagtaaac acaaaacata
     1861 agtattctca gtaataatgg attggatcga acgaaacata tcatctaatt gattcgcttt
     1921 tgtcaaggct acgatcaaat ccacgtgttt gttgctttcg atgatttctt ctggtgaata
     1981 gattggcagt tttgcaacta cttctttgcc attaaagtca gcaatcaatc catttgtacg
     2041 aatggcttca atgtgagcag gccagcgatc gatcaagacg acctcatttc cgctttgatg
     2101 caacattaat ccgattcggc tgcccattgc tccggctcca gctattgcga ttttcatgtt
     2161 ttttctctcc tttattcaat gagttccctt ttagtatata gaaaaacaga agaaaatagc
     2221 attgttttgt ttgaaaaaag tgaaaaaaag tgtcttactt aagaagaaag tgagggatat
     2281 agatggatat tttggcggca atcgattcat ttaaaggaag tgcttcttca tttgaattaa
     2341 atcaggcagt tctaaatggt tgggaagacc gtaaaggaag aaaaatcaat gtgccgattg
     2401 cagatggcgg agaaaatact gcagaagcaa tctattacgc gctcggggga acctggcgta
     2461 cgattgatgg gatggatctc ttatttcgtc agcgaaaaat cagttattta ttgacgaact
     2521 ggcaaggaaa aaataccgca gtcattgaat ctgcacaagt attaggatta gatttacttg
     2581 ataaaccaac ggatcaaacc atccgacttg cttctagtta tgccttggga gagcttatca
     2641 aagatacact gcttcaagaa gtggaacaaa tcattgtcac tttaggagga agcggatgta
     2701 gcgatggtgg tctaggatta ctccaatctt tgggtgcttc tttagaaggg tacacagaag
     2761 gaaatccttt gttatctact agaaaacttc atttggatcg aaggaacgtt cttgtttcta
     2821 aagtaaaagt gatcatcgct gcagacgtga ccaacccata tacaggtcaa caaggagctg
     2881 cttggcaatt tggaagacaa aaaggaggaa cggatacgac gttagctttc ttggataaac
     2941 aagctgaaaa gattgctcag cagataaaag atgtttggga ggtggattta gatgacatcc
     3001 caggaagcgg agcagcaggc ggtattggcg gagccctttt tttgttagga gcgaagatgg
     3061 tctctggttt tgagttggtc agccaaatca ctggattaga aaaacagatc gaacaagcgg
     3121 atgtcatcat tacaggagaa gggcggattg atcagcaaac aatccacgga aaagttccat
     3181 atggcgtagc agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta
     3241 gagaccgaga gctggaagga ctctttgatt ttctccccat tgtcttaagc attcaattag
     3301 gaccaatcga tttagaggaa gcaatggaac acaggcaaac attagaaaaa acagaaatct
     3361 taggacagac actcagcaga tttttcaaca ttctcccgaa gtagttgaca aaaacaattt
     3421 ctttatgcta tcgtaatttc aaatatagga caaagtgagg gatccgtgca ttgaaaaatt
     3481 aagtcgattg atgaaagctg cattcagact gtaccttgtg gagacaaggg agcagtgtgc
     3541 agattttcgg gattggcttg gttctttcga tgtatgggtc tttttgtgtg ccccaaccct
     3601 tgcatcctct ttagccagtt ctgaaaatag gactggtctt tttttacgag gtgatggaat
     3661 aatgtctaaa atcgaaataa aaaatctgac attcggctat gacagccaag gcacattatt
     3721 atttgaacaa gccaatctaa actttgacac acaatggaaa ctaggactta tcggacgaaa
     3781 cggtcgagga aagacaactt tactgaatat cctgcaaaac aaactccctt atcaagggca
     3841 agtgatccat cagcaagcgt ttgtctattt cccgcagcaa acaaaagata aagagcgttt
     3901 gacctattat gtgttaaatg agattatgga ttttgagata tgggaaattg aaagagagct
     3961 ccaactgatg caaacagatc ctgaaatctt atggagagaa ttcagcacac tatcaggagg
     4021 agagaagaca aaagtcctac tggcactctt atttgtggat gacactcatt tcccgttaat
     4081 cgatgaacca acgaatcatt tggatatctc tggcagaaaa caagtagcgg cttacttgaa
     4141 aaagaaaaaa caaggattca tcgtggtgag tcatgatcgg ggatttatcg atgaagtagt
     4201 ggaccatgtt ttagcaatcg aaaagagcca actggaactt tatcaaggga atttctctat
     4261 ctatgaagaa cagaaaaaac ttcgtgatga atttgaaata gctaaaaatg aaaaattgaa
     4321 aaaagaagtc agcaggctaa agaaaacagc agctgaaaaa gcagaatggt ctcgttcccg
     4381 agaaggggac aaaacaaaga aaaaagtcgg attcatcgat acggaatcta ggcgagtgaa
     4441 taaaggagca gtaggcgctg atgctgcacg gacgatgaaa cgatctaaag caatcgtgaa
     4501 tcggatggaa acccagatca gtgagaaaga aaaattatta aaagatatcg agtatatcga
     4561 tgctttgacg atgaatagcc aagtgtctca tcataaacgg ctgttaagcg tagaaaatct
     4621 tcaattaggg tatgaaaatc tgttattcga gccagttcat tttacgatcg aacctcatca
     4681 gcggatggca atttcaggtc ctaacggcgc gggaaagtca tccattatcc attatcttct
     4741 ggggacattc aacggcaagg ttgtaggaga aaaatcccag cccaaacatt tgaccattag
     4801 ttacgtacgc caaaattatg aagacaatcg agggacattg gcggaattcg cgaagaaaaa
     4861 ccgagtagac tatcaagcat ttttgaataa tctccgaaag cttgggatgg aaagagatgt
     4921 ttttcataat aaaatcgaac agatgagtat gggacaacgg aaaaaagtgg aattggctaa
     4981 atctttatcc caaccagctg agctctatat atgggatgaa ccattgaatt atttggatgt
     5041 cttcaatcaa gaacaattag aacaactgat tttgaacgtg aaacctgcta tgttactagt
     5101 ggaacatgat caaacctttc tagataaagt atctactgag attatttctc ttgagaaaat
     5161 ctaaaccttg taatctcaaa gacaacaagg tatggttaag gagaactttg tcaacgaagg
     5221 agagaatcac atgctttatg taacgagaca cggagaaaca acttggaacg cacaaggatt
     5281 agtttgcggg cgagcagatg tgccgctgac tgaaaaagga cagatgcagg cacaggcact
     5341 agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa atcatccatt cccctcttca
     5401 acgagcaaga gatacagcac aagcagtagt tgatcgtctc aatttgccga tgaccgctga
     5461 tgagcgattg gtagaaatgg attttgggga ttatgacgga ctgccaagca aagatgaaaa
     5521 ctttcaaaaa gcgcgtttag cgtttgctgt acgtttccca aatggagagt cggtcttaga
     5581 tgtgtacgca cggatcgttc ctttattgaa agaatgcatg gaagacgaag aaaatgtcta
     5641 cttgttagtc tgtcataatg ccttgataag agtgatcaat gcttattttc atccaatgcc
     5701 taatgaaggg ttctttacat tcatggtaga caatacagaa ttaatttctt atgagtaatt
     5761 agacttttgt gttatttaaa taaaatgttt caaggcactg cttttgctta gcagtgcctt
     5821 ttttgtggct gtgtcaattc ttttggcatt ataaatccaa tttttagaaa taaaatttaa
     5881 ttattttaac aaaagtattg aaattt
//
LOCUS       DAEGIQ010000324         5906 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.141, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000324 DAEGIQ010000000
VERSION     DAEGIQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.141"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            177..1073
                     /locus_tag="KMT45_003137"
     CDS             177..1073
                     /locus_tag="KMT45_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBE7006495.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(1216..2157)
                     /locus_tag="KMT45_003138"
     CDS             complement(1216..2157)
                     /locus_tag="KMT45_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBE7006496.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2283..3404
                     /locus_tag="KMT45_003139"
     CDS             2283..3404
                     /locus_tag="KMT45_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBE7006497.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3662..5164
                     /gene="eat(A)"
                     /locus_tag="KMT45_003140"
     CDS             3662..5164
                     /gene="eat(A)"
                     /locus_tag="KMT45_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBE7006498.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKRQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5192..5758
                     /locus_tag="KMT45_003141"
     CDS             5192..5758
                     /locus_tag="KMT45_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBE7006499.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
BASE COUNT         1935 a         1038 c         1239 g         1694 t
ORIGIN      
        1 aattacaact attcagaaaa cttttgggca tattttaatg gaagaatgat ttatttcatc
       61 gtattttcat agaaatgtta aatttttata aaaataatag gaagcgtttt actggctttt
      121 tactatttaa acaggctatc ataggaaaaa gtgacagaga aagtgaggcg gaacagatga
      181 acaaaaacga cgtattgcta attgcgaaaa ataaatcact ttattttttt ccttccatcc
      241 gctctttaga tgtttggtcc ggaaaagatt tactacagtc ggaattcatt aaatcagctc
      301 gacaacagtt ggcaaattat cagctgatcg tttttttaga ttatggattt gatatggaga
      361 tggctcgacg cgtgcgtcca tttaccaatg caaaaatcgt gttatttttt tggaaccact
      421 ttaaagcaga acacgaggcg atgctgaagc aggcacaaga tgaaccggca atcgatgaga
      481 tttatcattt tgatttttta gaagcgaaag aacttggact aaaacataat agttcctttt
      541 attcgaaaca tatggatttt tcttctagcg aaccaaagac ggatctattt tttggtgcaa
      601 caaataacgg cagaaaagaa cgggcagaaa aatataagca ggaatttaca aaaagaggtg
      661 ttacgaccaa ttactttatt cttccgcttc gaggaaatga acaacctgga taccttactt
      721 atgaagaata tctgaaaaaa accggggaat cgagggggat tctagaactg ttgcgggaag
      781 ggcagcaagg agtgactttg cgaacgtttg aatctatgta ttttcaaaag aaactagtga
      841 cagataatca agccatagct cattatcagt tttatcattc tgaaaatatt tttttgcttc
      901 aagaacgtga tttagatgaa ttaccggaat ttttgcatac gccatttcaa ccaatcccat
      961 cagaactgtt ggaatttttt gatgcagaaa attgggcaaa acgttttttc actactgact
     1021 ccgctattta tgaacaatat gaatacgatc tttcctctgt taaaaataaa taaaagtgcc
     1081 aaccaatcat agatgaaaat aaacaagcgc aattcttatg gcaacatgag aattgtgttt
     1141 ttctttttga actttcatgg gggtaaaaaa atgagaacca gctgctgatc cagctgattc
     1201 tcatcagaga gccttttatt ctgcgccaag gattccttct tttgcatgga ctaattgggt
     1261 caaaaaggca caatatggtg tagcaatatc atatttttgt ccttttcgcc aaacggcacc
     1321 attgatgtag tcgatttcag tcaaacgatg gtttttgatc aggtcttggt acatagatgg
     1381 gtaatgcaac ccgatgttgt caggatcata agtagaagca atatgttggt acacttcttc
     1441 ttgatctaat gtgattcctt cttttgctgc gatagaagca aactcagata caatcgttcg
     1501 aaccatgccc tctgccgctt tttgcgcacc taattcagca atattacaat ccaatatcgt
     1561 gcataatcca tttaatgttc cgtttacaca agcttttcgc caaatggaat aacgaacatt
     1621 atgactatag attggattca attttgcttc ttgaaagact tctacaactt tttttgtaaa
     1681 tgcttcgcct tctggttcca agttctctag ttcgatttct ccatcaccta ataatgtgac
     1741 ttttccggga ccagctagac cagctgtcca catcgtgatc ccaaataaga tatttttctt
     1801 tggtacatat ttttccagta catcttcatg acctaatcca ttcagtaaac acaaaacata
     1861 agtattctca gtaataatgg attggatcga acgaaacata tcatctaatt gattcgcttt
     1921 tgtcaaggct acgatcaaat ccacgtgttt gttgctttcg atgatttctt ctggtgaata
     1981 gattggcagt tttgcaacta cttctttgcc attaaagtca gcaatcaatc catttgtacg
     2041 aatggcttca atgtgagcag gccagcgatc gatcaagacg acctcatttc cgctttgatg
     2101 caacattaat ccgattcggc tgcccattgc tccggctcca gctattgcga ttttcatgtt
     2161 ttttctctcc tttattcaat gagttccctt ttagtatata gaaaaacaga agaaaatagc
     2221 attgttttgt ttgaaaaaag tgaaaaaaag tgtcttactt aagaagaaag tgagggatat
     2281 agatggatat tttggcggca atcgattcat ttaaaggaag tgcttcttca tttgaattaa
     2341 atcaggcagt tctaaatggt tgggaagacc gtaaaggaag aaaaatcaat gtgccgattg
     2401 cagatggcgg agaaaatact gcagaagcaa tctattacgc gctcggggga acctggcgta
     2461 cgattgatgg gatggatctc ttatttcgtc agcgaaaaat cagttattta ttgacgaact
     2521 ggcaaggaaa aaataccgca gtcattgaat ctgcacaagt attaggatta gatttacttg
     2581 ataaaccaac ggatcaaacc atccgacttg cttctagtta tgccttggga gagcttatca
     2641 aagatacact gcttcaagaa gtggaacaaa tcattgtcac tttaggagga agcggatgta
     2701 gcgatggtgg tctaggatta ctccaatctt tgggtgcttc tttagaaggg tacacagaag
     2761 gaaatccttt gttatctact agaaaacttc atttggatcg aaggaacgtt cttgtttcta
     2821 aagtaaaagt gatcatcgct gcagacgtga ccaacccata tacaggtcaa caaggagctg
     2881 cttggcaatt tggaagacaa aaaggaggaa cggatacgac gttagctttc ttggataaac
     2941 aagctgaaaa gattgctcag cagataaaag atgtttggga ggtggattta gatgacatcc
     3001 caggaagcgg agcagcaggc ggtattggcg gagccctttt tttgttagga gcgaagatgg
     3061 tctctggttt tgagttggtc agccaaatca ctggattaga aaaacagatc gaacaagcgg
     3121 atgtcatcat tacaggagaa gggcggattg atcagcaaac aatccacgga aaagttccat
     3181 atggcgtagc agtgcttgca aaaaaatatc gaaaaccaat aatcgctatt tgcggaagta
     3241 gagaccgaga gctggaagga ctctttgatt ttctccccat tgtcttaagc attcaattag
     3301 gaccaatcga tttagaggaa gcaatggaac acaggcaaac attagaaaaa acagaaatct
     3361 taggacagac actcagcaga tttttcaaca ttctcccgaa gtagttgaca aaaacaattt
     3421 ctttatgcta tcgtaatttc aaatatagga caaagtgagg gatccgtgca ttgaaaaatt
     3481 aagtcgattg atgaaagctg cattcagact gtaccttgtg gagacaaggg agcagtgtgc
     3541 agattttcgg gattggcttg gttctttcga tgtatgggtc tttttgtgtg ccccaaccct
     3601 tgcatcctct ttagccagtt ctgaaaatag gactggtctt tttttacgag gtgatggaat
     3661 aatgtctaaa atcgaaataa aaaatctgac attcggctat gacagccaag gcacattatt
     3721 atttgaacaa gccaatctaa actttgacac acaatggaaa ctaggactta tcggacgaaa
     3781 cggtcgagga aagacaactt tactgaatat cctgcaaaac aaactccctt atcaagggca
     3841 agtgatccat cagcaagcgt ttgtctattt cccgcagcaa acaaaagata aagagcgttt
     3901 gacctattat gtgttaaatg agattatgga ttttgagata tgggaaattg aaagagagct
     3961 ccaactgatg caaacagatc ctgaaatctt atggagagaa ttcagcacac tatcaggagg
     4021 agagaagaca aaagtcctac tggcactctt atttgtggat gacactcatt tcccgttaat
     4081 cgatgaacca acgaatcatt tggatatctc tggcagaaaa caagtagcgg cttacttgaa
     4141 aaagaaaaaa caaggattca tcgtggtgag tcatgatcgg ggatttatcg atgaagtagt
     4201 ggaccatgtt ttagcaatcg aaaagcgcca actggaactt tatcaaggga atttctctat
     4261 ctatgaagaa cagaaaaaac ttcgtgatga atttgaaata gctaaaaatg aaaaattgaa
     4321 aaaagaagtc agcaggctaa agaaaacagc agctgaaaaa gcagaatggt ctcgttcccg
     4381 agaaggggac aaaacaaaga aaaaagtcgg attcatcgat acggaatcta ggcgagtgaa
     4441 taaaggagca gtaggcgctg atgctgcacg gacgatgaaa cgatctaaag caatcgtgaa
     4501 tcggatggaa acccagatca gtgagaaaga aaaattatta aaagatatcg agtatatcga
     4561 tgctttgacg atgaatagcc aagtgtctca tcataaacgg ctgttaagcg tagaaaatct
     4621 tcaattaggg tatgaaaatc tgttattcga gccagttcat tttacgatcg aacctcatca
     4681 gcggatggca atttcaggtc ctaacggcgc gggaaagtca tccattatcc attatcttct
     4741 ggggacattc aacggcaagg ttgtaggaga aaaatcccag cccaaacatt tgaccattag
     4801 ttacgtacgc caaaattatg aagacaatcg agggacattg gcggaattcg cgaagaaaaa
     4861 ccgagtagac tatcaagcat ttttgaataa tctccgaaag cttgggatgg aaagagatgt
     4921 ttttcataat aaaatcgaac agatgagtat gggacaacgg aaaaaagtgg aattggctaa
     4981 atctttatcc caaccagctg agctctatat atgggatgaa ccattgaatt atttggatgt
     5041 cttcaatcaa gaacaattag aacaactgat tttgaacgtg aaacctgcta tgttactagt
     5101 ggaacatgat caaacctttc tagataaagt atctactgag attatttctc ttgagaaaat
     5161 ctaaaccttg taatctcaaa gacaacaagg tatggttaag gagaactttg tcaacgaagg
     5221 agagaatcac atgctttatg taacgagaca cggagaaaca acttggaacg cacaaggatt
     5281 agtttgcggg cgagcagatg tgccgctgac tgaaaaagga cagatgcagg cacaggcact
     5341 agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa atcatccatt cccctcttca
     5401 acgagcaaga gatacagcac aagcagtagt tgatcgtctc aatttgccga tgaccgctga
     5461 tgagcgattg gtagaaatgg attttgggga ttatgacgga ctgccaagca aagatgaaaa
     5521 ctttcaaaaa gcgcgtttag cgtttgctgt acgtttccca aatggagagt cggtcttaga
     5581 tgtgtacgca cggatcgttc ctttattgaa agaatgcatg gaagacgaag aaaatgtcta
     5641 cttgttagtc tgtcataatg ccttgataag agtgatcaat gcttattttc atccaatgcc
     5701 taatgaaggg ttctttacat tcatggtaga caatacagaa ttaatttctt atgagtaatt
     5761 agacttttgt gttatttaaa taaaatgttt caaggcactg cttttgctta gcagtgcctt
     5821 ttttgtggct gtgtcaattc ttttggcatt ataaatccaa tttttagaaa taaaatttaa
     5881 ttattttaac aaaagtattg aaattt
//
LOCUS       DAEGIQ010000325         4768 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.166, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000325 DAEGIQ010000000
VERSION     DAEGIQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.166"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..914
                     /locus_tag="KMT45_003142"
     CDS             <1..914
                     /locus_tag="KMT45_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBE7006500.1"
                     /translation="AGVILVTLVIGIIVLPMLTETEEAESTDLNALMILEEVVEVLRK
                     EIKEIDRNTKEFLATEAVIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLD
                     ESYRSGKLSSNGYRFYSRFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQR
                     RYADRQTFISDEDIQEIRETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKM
                     NSGNLISSWMIQQDSLFTKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLES
                     YTMNKPSDNFSFRFAFRAKSKKTSPIK"
     gene            1074..2960
                     /locus_tag="KMT45_003143"
     CDS             1074..2960
                     /locus_tag="KMT45_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBE7006501.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2972..3919
                     /locus_tag="KMT45_003144"
     CDS             2972..3919
                     /locus_tag="KMT45_003144"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBE7006502.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3939..4466
                     /locus_tag="KMT45_003145"
     CDS             3939..4466
                     /locus_tag="KMT45_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBE7006503.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4529..>4768)
                     /locus_tag="KMT45_003146"
     CDS             complement(4529..>4768)
                     /locus_tag="KMT45_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBE7006504.1"
                     /translation="SKVSTIIYIFMGWLCVVAAVPLYHSLGMTGVILMVAGGLSYTIG
                     AFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         1673 a          800 c         1027 g         1268 t
ORIGIN      
        1 cggcgggtgt catcttagtg actttggtca tagggatcat tgtcttgcct atgctgacgg
       61 aaacagagga agcggaaagc acggatttga atgctttgat gattttagaa gaggtcgttg
      121 aagttttgcg aaaagaaata aaggaaattg atcgtaatac aaaagaattt ttagccactg
      181 aagcagtgat agaaaattat caagaacgga tacgtgatct gtacttggaa gatttaaccg
      241 atgatgaaaa gcaagaagta caagaaatac aagcactgat tttgtcgatc gaacgggatg
      301 gattggatga aagttatcgt tcgggcaagc tttcttctaa tggttatcgt ttttactccc
      361 gttttctttc tcgattcgaa cattctatca caagccagat tttgtctttt atcggctttt
      421 ggttcattgt tgtccgtagg ttaatgcgaa tcgttctcca tccgaaaatg ttttggcaaa
      481 gaagatatgc tgatcgtcag acatttatta gtgatgaaga tatccaagaa atcagagaga
      541 catatcaaaa aaatactcag ttgattattg aaagtttaga taacttgact gatatttatg
      601 atgaaacgat ggttcaattt tttatccagc aacgcaaact tgaaggaata aaaatgaact
      661 cggggaattt gatttcttcg tggatgatcc agcaagatag tctgtttacg aaaaaaatgc
      721 taagaggtta ttatcttgaa agaaaaatca ttgatgaata cgaagttgct gaaaaaatca
      781 cgacgttttc tgcgaacagc tatcgcagga atatcaattt gttagaatct tatactatga
      841 acaaaccatc tgataacttt tctttccgat ttgcgtttcg agcaaagtca aaaaaaacga
      901 gtccaataaa ataaaaaggt aaaaattttc aagttttata aaaaacaaaa catatcagaa
      961 caggatcgaa tcggtcctgt tctttttttg agaacacaaa aagaacaaat accaaaaaaa
     1021 ttttcctgaa gttcactatt tttatgatac aatatccaag ataaggaggg aacatgttga
     1081 aagaattaaa agtaactgac ctcaaaaaaa cttatggtga aaaagatttg tttgaccaga
     1141 tttctttttt ggtccacgaa aaagaccgta tcgggttgat tgggacgaac gggacgggta
     1201 aaacaagtct cttaaatatt attgccggta tcgatagcag cgacggtgat cggcaaacag
     1261 tgttttatcc aactgattac cgtattggtt atctttctca agcagctgaa ttttccgatg
     1321 aattaactgt tctccaagca gtctttcaag gaaacagccc cttgatccaa actgttcgag
     1381 catatgagga agcattgatc gaactagggg aaaacggaga agaccctgat gtgcaaaaac
     1441 gttatgctaa agcagaagaa caaatgaata aagaagatgc ttggactaca gataccaacg
     1501 caaagattat tttgcaaaaa ttaggaatca gtgaattaga taaaaaaatc agtactttat
     1561 caggtggtca gaaaaaacga gttagtctgg cgcaagtgtt gattgatgaa cccgatttac
     1621 tattactaga cgaaccaaca aaccatttag attatgaagc gatcgagtgg ttggaaaact
     1681 atttgaaaca ataccgtggc gcactattga tggttaccca cgatcgttat tttctagata
     1741 gagtagcaaa ccgaatcttc gaactttcat ttggaaaatt atacgagtat aaaggaaatt
     1801 atgaagctta cgtcttagaa aaagcagaac gtgatcgtgt agaagtagaa caagaagaaa
     1861 agcgcaagcg gctttataaa caggaattag cttggatgag ggctggcgta caggcacgtg
     1921 gcaccaagca gcaagcacgg atcaaccgtt tcgaagatct aaaggagaac ttgtaccaag
     1981 taaaccaaga agatgattta gagctgaacc tagcgacaca acgtttaggg aaaaaagtac
     2041 tggaaatcaa agatggcagt taccgtataa atgaccaaac actgctagaa catttagatt
     2101 tactgattca atctcgggaa agactaggta ttactggtaa aaatggtgcg ggaaaatcga
     2161 cattgcttaa tattctagct ggcagaattc ccctggatag cggtacaatg tctatcggtg
     2221 aaacggttca tcttgcgtat tatacacaag aaaatgaaga aatggcacca gataaacgga
     2281 tgattgctta cttacaagaa gctgctgaag aagcaaaaac agcagatggc agtcaaatcg
     2341 gagtagcaga attacttgaa cgatttttat ttccaagatt tatgcatggg acactgatag
     2401 gcaaactttc aggcggagaa aaacgccggt tatttttgtt gaaactgctg attcagcaac
     2461 ctaatgtttt attattggat gaaccgacca atgatttgga catagcaaca ttgacgattt
     2521 tggaagatta ttttagaagt tttccaggtg ctgtgatcac tgtttcccat gaccgttatt
     2581 tcttggacaa ggtagcagat aagttattag tattccaagg aaacggaaaa caagaacttt
     2641 attacggaaa catgtccagt tatttactga agcaaaaaga gacccagcag cctgctgaaa
     2701 aagcgaaacc aaaaatacag agcaaggaac ctgctgaaaa gaagaaatta tcttacatgg
     2761 aacaaaaaga atgggaaacg attgaagatg agattgcaga acttgaagaa aaaatctcat
     2821 tgctccaaga agagatgaat catcaaggag ataatttcac tcgattacaa gaattgcaaa
     2881 acgacgtatc tgaaacagaa gcacaacttg aagaaaaaat ggcacggtgg gaatacttga
     2941 gtgaatgggt agaagattag gaggaaaaag gatggaacaa gcgtatcttg acttaggaag
     3001 aaaattgttg gaagaagggc atcgtaaaga agacagaaca ggcacaggca cattaagctt
     3061 attcggatac caaatgcggt ttgatctgca aaaagggttt cctttgttaa caacgaaacg
     3121 agtacctttt ggattgatca aaagcgaatt gctttggttt ttaaaaggag atacgaatat
     3181 ccgctatcta ttacaaaata aaaaccatat ttgggatgaa tgggcatttg aacgctacat
     3241 caaaagtcca gactataatg gaccagatat gactgatttt ggccatagat gtttgacaga
     3301 tcctgaattc aatgaagaat ataaaaagca gagcaagctt ttttgcgaga agatcctaac
     3361 cgatgattcc tttgctgaaa catacggcga tcttggacac atctacggct atcaatggcg
     3421 tcactgggaa acaaaagacg gtggctttat cgatcaaatc aaacaagtga tcgaagcaat
     3481 caaaaagact cctgattcta gaagattgat cgtttctgca tggaatccgg aagatgttcc
     3541 tagcatggct ttgcctcctt gtcatacgat gtttcaattt tatgttcaag agggacggct
     3601 aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta ggcgttccgt tcaacattgc
     3661 aagttatgcg ttgctgactc acctaattgc acatgaaaca ggtttagcgg ttggcgaatt
     3721 tgttcacaca ttaggagatg ctcatcttta tatcaatcat atcgaacaaa tgaaagaaca
     3781 attgagccga gaaatacgcg catttccgac tttagttttg aatcaggaaa agcagtcagt
     3841 atttgatttt gagatggaag atatccaatt agaaggatat gacccacatc cggcgatcaa
     3901 agctcctatc gctgtttaga atcagaaagg atgagaaaat gttagctgca atttgggcac
     3961 aggatgaaaa cggattgatt ggaaaagaag accgtcttcc ttggcgtttg ccaaatgatc
     4021 tgaaattctt caaacaaatg acagaagcaa atacactagt gatgggacga aaaacattcg
     4081 aagggatggg tagccgtccg ttgccgaatc gtcagacgat tgttttgacg agagattctt
     4141 cctataaagc agaaggcgtg catgtcatgc atagtgtgga agacgtgctt gctctggaaa
     4201 aagaaacaga cggtatcttt ttcatcgctg gagggtcagc tgtttatcaa gaatttctgc
     4261 cattttgtac gatattatac cgtacagtca tccatcatgt attcgatgga gatgcttatt
     4321 ttccacctgt tgactggtct gattggtcac tgatcaatac tagtcaagga gaaatggatg
     4381 aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg taatgagaat cctgtaaaaa
     4441 gcgggacagg cgaattgacg atataaaaaa gctatggata gactagctgt tatttagagc
     4501 tgtcccatcc atagctttat tttttttatt aagtaaagaa taaaacagag aagtacataa
     4561 aacctgcacc taacataaca aacaaatgcc aaacaacatg catatagcgg atagaattca
     4621 agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc tgctaccatc aaaataacac
     4681 cagtcattcc aagagagtga taaagaggaa cagcagccac gacacagagc caccccataa
     4741 aaatatagat gatcgtagat acttttga
//
LOCUS       DAEGIQ010000326         4667 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.167, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000326 DAEGIQ010000000
VERSION     DAEGIQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.167"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            166..1077
                     /gene="vanY"
                     /locus_tag="KMT45_003147"
     CDS             166..1077
                     /gene="vanY"
                     /locus_tag="KMT45_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBE7006505.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1230..1715
                     /gene="vanZ"
                     /locus_tag="KMT45_003148"
     CDS             1230..1715
                     /gene="vanZ"
                     /locus_tag="KMT45_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBE7006506.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFELVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2002..2331)
                     /locus_tag="KMT45_003149"
     CDS             complement(2002..2331)
                     /locus_tag="KMT45_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7006507.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2636..2965
                     /locus_tag="KMT45_003150"
     CDS             2636..2965
                     /locus_tag="KMT45_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE7006508.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2955..3242
                     /locus_tag="KMT45_003151"
     CDS             2955..3242
                     /locus_tag="KMT45_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE7006509.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3521..4084)
                     /locus_tag="KMT45_003152"
     CDS             complement(3521..4084)
                     /locus_tag="KMT45_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7006510.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1649 a          695 c          789 g         1534 t
ORIGIN      
        1 aaaattcatc tacattggtg ataatagtaa atccagtagg gcgaaataat tgactgtaat
       61 ttacggggca aaacggcaca atctcaaacg agattgtgcc gtttaagggg aagattctag
      121 aaatatttca tacttccaac tatatagtta aggaggagac tgaaaatgaa gaagttgttt
      181 tttttattgt tattgttatt cttaatatac ttaggttatg actacgttaa tgaagcactg
      241 ttttctcagg aaaaagtcga atttcaaaat tatgatcaaa atcccaaaga acatttagaa
      301 aatagtggga cttctgaaaa tacccaagag aaaacaatta cagaagaaca ggtttatcaa
      361 ggaaatctgc tattaatcaa tagtaaatat cctgttcgcc aagaaagtgt gaagtcagat
      421 atcgtgaatt tatctaaaca tgacgaatta ataaatggat acgggttgct tgatagtaat
      481 atttatatgt caaaagaaat agcacaaaaa ttttcagaga tggtcaatga tgctgtaaag
      541 ggtggcgtta gtcattttat tattaatagt ggctatcgag actttgatga gcaaagtgtg
      601 ctttaccaag aaatgggggc tgagtatgcc ttaccagcag gttatagtga gcataattca
      661 ggtttatcac tagatgtagg atcaagcttg acgaaaatgg aacgagcccc tgaaggaaag
      721 tggatagaag aaaatgcttg gaaatacggg ttcattttac gttatccaga ggacaaaaca
      781 gagttaacag gaattcaata tgaactatgg catattcgct atgttggttt accacatagt
      841 gcgattatga aagaaaagaa tttcgttctc gaggaatata tggattacct aaaagaagaa
      901 aaaaccattt ctgttagtgt aaatggggaa aaatatgaga tcttttatta tcctgttact
      961 aaaaatacca ccattcatgt gccgactaat cttcgttatg agatatcagg aaacaatata
     1021 gacggtgtaa ttgtgacagt gtttcccgga tcaacacata ctaattcaag gaggtaagga
     1081 tggcggaatg aaaccaacga aattaatgaa cagcattatt gtactagcac ttttggggta
     1141 acgttagctt tttaatttaa aacccacgtt aactaggaca ttgctatact aatgatacaa
     1201 cttaaacaaa agaattagag gaaattatat tgggaaaaat attatctaga ggattgctag
     1261 ctttatattt agtgacacta atctggttag tgttattcaa attacaatac aatattttat
     1321 cagtatttaa ttatcatcaa agaagtctta acttgactcc atttactgct actgggaatt
     1381 tcagagagat gatagataat gttataatct ttattccatt tggcttgctt ttgaatgtca
     1441 attttaaaga aatcggattt ttacctaagt ttgcttttga actggtttta agtcttactt
     1501 ttgaaataat tcaatttatc ttcgctattg gagcgacaga cataacagat gtaattacaa
     1561 atactgttgg aggctttctt ggactgaaat tatatggttt aagcaataag catatgaatc
     1621 aaaaaaaatt agacagagtt attatttttg taggtatact tttgctcgta ttattgctcg
     1681 tttaccgtac ccatttaaga ataaattacg tgtaagatgt ctaaatcaag caatctgatc
     1741 tttcatacac ataaagatat tgaatgaatt ggattagatg gaaaacggga tgtggggaaa
     1801 ctcgcccgta ggtgtgaagt gaggggaaaa ccggtgataa agtaaaaagc ttacctaaca
     1861 ctatagtaac aaagaaagcc caattatcaa ttttagtgct gaggaattgg tctctttaat
     1921 aaatttcctt aacgttgtaa atccgcattt tcctgacggt acccccaaac taagatttca
     1981 tagatagatt ttctgataac tttaaaaaac acgaatagcg gaaaggatgt cttcattcgc
     2041 taaagagtaa taaactactt ttccatcttg acgtgatttt gccattccat gtttttttaa
     2101 aaaacgtaaa tggtgtgacg ttgtagcgac actagcagta agtatttctg ctaaatcaca
     2161 gacgcacatt tccttatagg tttctaaagc atagaaaatt ttaattctag aaaaatcgct
     2221 gaaacatttc cctaacacaa gtaagtttga aaaatctttt tgatccaatt tattttttat
     2281 tccatttact ttttctttat gtacgacagt cacttggcaa acttttttca ttcatctcac
     2341 ctcacattca aacgattatt tgaatgaagt atacctattt gatcactctt ttgtcaatct
     2401 accttaattc caatacacta ttataagcaa tttcatgtat tgaagtaaac ttacttgcaa
     2461 gtgcgttttt gattccaagt taaaaacata ttattattct acataatttc ctaatcaaat
     2521 aaaactcctt tgaaaattgt tctaaaggag ttttattatt ttgtatttct actaaaaata
     2581 gcgtacaatg taattactaa gtaattacga aatgtaaaat aatggaggaa ataatatgat
     2641 tacaaaaaca agaaaacaag gaaattctat tatgttaacg gtccctaaag aattcgatgt
     2701 gcccaatggt gttgaagtag aagctaagct tgtagagaat ggtatccttt atgagtttgt
     2761 agagcctaaa aaagaatttt ttgattttag tgaagatatt ttggctgata ttctttcaga
     2821 aggttatagc aaacaagaga tcttaaaaga atttagaaat agaaaaaatg cgttaacttc
     2881 tgcatttaaa tctattgctg aagatactgt tgcaaattct aaaccgatga caaaagagga
     2941 gttggcagca gaaattggct tatgagattc taccttctaa acacgtaata aagtatctaa
     3001 aaaaattaaa agagaaaaca ttaaaagaac aatttttaac gataatttat gatgagatag
     3061 cagttcgtcc tcattcggga gaacaaaaaa cgggtgattt atcaggaatt tgggctattg
     3121 gattcaagta tgcaggtacg acatatcgcg ttgcatatga aattaaagat aatgcagtga
     3181 tacctatctt actatgtgga acgcatgaaa atttttatga acaattaaaa aagattaggt
     3241 aagaacccaa tgtttcaact tgagaggata gacgacactt taaaagataa tttttaacag
     3301 ctgagagttg taatacccta taattttaaa ccactaacct atatatttat gaaaaataga
     3361 aaaaattaaa acgcatttta agcctctttc aaattaaaag cttcatcctt atgcacaaag
     3421 agggcttaaa atgcgttatt ttatattgtt ttttagtaat aataaaaaaa tcactctaat
     3481 tctcccataa atattataga ttcctaaaga ttaaaataga ttattcccta ttgtctttta
     3541 agtagcggta gagtgttgcc ttgctaatac cagtcatttc cgtaatttct tttacgctat
     3601 aaacctgact tttatacaat tttactgctc gatcaacatc tttctggttc actcgtggac
     3661 ggccaccttt acgacctctc gcacgtgcac ttttcaaacc ttctttggta cgttcaacaa
     3721 ttaaatcacg ttcgaactga ctaaatgctt ggaagacagt catcattaag cgtccgtgag
     3781 gcgtgctggt atcgaagttt tctttcaagc tcaccagctt aacatggtgt tcttcaaaat
     3841 aggtaacaag gtcaatcaaa tcctttgtac tacgacccaa tcgagaaaaa ctttctacaa
     3901 ttaccgtatc tcccggacga attttatctt ttaaacgatt tagttgggga cgaaccgctt
     3961 tggttcccgt cattttctca gttaggattt cattacaatt ttgttcattg agtaagtcta
     4021 attgacgatt tagttcttgt gaacgtgtac tcactcgggc ataaccataa atgtatccac
     4081 tcatgctttt cttcctttcg tactttaatt ctggcttgat tgtatcataa aagggagtta
     4141 tagatacata gttaatgcaa caagttatga tacaaaatat gacctctttt atttaataaa
     4201 agaggtcatt tcattttgtc tcataatcga ttgtttttga tacgagtgtg tatacgaaat
     4261 aaacaaaaaa atcttaagta aatttctaga tacctaattt aatcctaact agtgtatcct
     4321 aaagttaata acgagtttat cacaatgaaa attataaaat gctcccatat tagatataac
     4381 atgcaatatt tgaattagaa ctacaaaatt tattcgtttt ataaagtaaa taatgttgag
     4441 atactaagga atagaatatt ataaggaatt gagctgaatg tattggataa aaaaagaata
     4501 actttattag agatggacta taaaaatgct cgtaattttc ttctgaagtc tgaaagctat
     4561 ttgaatgctc agctaccaga atatattagt tttgacgaaa ctttaaaatc tgctaaaaaa
     4621 gttttaactt cgccgtttgt ataattaagc gagacaaata aaaaagc
//
LOCUS       DAEGIQ010000327         4667 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.168, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000327 DAEGIQ010000000
VERSION     DAEGIQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.168"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            166..1077
                     /gene="vanY"
                     /locus_tag="KMT45_003153"
     CDS             166..1077
                     /gene="vanY"
                     /locus_tag="KMT45_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HBE7006511.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1230..1715
                     /gene="vanZ"
                     /locus_tag="KMT45_003154"
     CDS             1230..1715
                     /gene="vanZ"
                     /locus_tag="KMT45_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBE7006512.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2002..2331)
                     /locus_tag="KMT45_003155"
     CDS             complement(2002..2331)
                     /locus_tag="KMT45_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE7006513.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2636..2965
                     /locus_tag="KMT45_003156"
     CDS             2636..2965
                     /locus_tag="KMT45_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE7006514.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2955..3242
                     /locus_tag="KMT45_003157"
     CDS             2955..3242
                     /locus_tag="KMT45_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE7006515.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3521..4084)
                     /locus_tag="KMT45_003158"
     CDS             complement(3521..4084)
                     /locus_tag="KMT45_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE7006516.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1648 a          695 c          789 g         1535 t
ORIGIN      
        1 aaaattcatc tacattggtg ataatagtaa atccagtagg gcgaaataat tgactgtaat
       61 ttacggggca aaacggcaca atctcaaacg agattgtgcc gtttaagggg aagattctag
      121 aaatatttca tacttccaac tatatagtta aggaggagac tgaaaatgaa gaagttgttt
      181 tttttattgt tattgttatt cttaatatac ttaggttatg actacgttaa tgaagcactg
      241 ttttctcagg aaaaagtcga atttcaaaat tatgatcaaa atcccaaaga acatttagaa
      301 aatagtggga cttctgaaaa tacccaagag aaaacaatta cagaagaaca ggtttatcaa
      361 ggaaatctgc tattaatcaa tagtaaatat cctgttcgcc aagaaagtgt gaagtcagat
      421 atcgtgaatt tatctaaaca tgacgaatta ataaatggat acgggttgct tgatagtaat
      481 atttatatgt caaaagaaat agcacaaaaa ttttcagaga tggtcaatga tgctgtaaag
      541 ggtggcgtta gtcattttat tattaatagt ggctatcgag actttgatga gcaaagtgtg
      601 ctttaccaag aaatgggggc tgagtatgcc ttaccagcag gttatagtga gcataattca
      661 ggtttatcac tagatgtagg atcaagcttg acgaaaatgg aacgagcccc tgaaggaaag
      721 tggatagaag aaaatgcttg gaaatacggg ttcattttac gttatccaga ggacaaaaca
      781 gagttaacag gaattcaata tgaactatgg catattcgct atgttggttt accacatagt
      841 gcgattatga aagaaaagaa tttcgttctc gaggaatata tggattacct aaaagaagaa
      901 aaaaccattt ctgttagtgt aaatggggaa aaatatgaga tcttttatta tcctgttact
      961 aaaaatacca ccattcatgt gccgactaat cttcgttatg agatatcagg aaacaatata
     1021 gacggtgtaa ttgtgacagt gtttcccgga tcaacacata ctaattcaag gaggtaagga
     1081 tggcggaatg aaaccaacga aattaatgaa cagcattatt gtactagcac ttttggggta
     1141 acgttagctt tttaatttaa aacccacgtt aactaggaca ttgctatact aatgatacaa
     1201 cttaaacaaa agaattagag gaaattatat tgggaaaaat attatctaga ggattgctag
     1261 ctttatattt agtgacacta atctggttag tgttattcaa attacaatac aatattttat
     1321 cagtatttaa ttatcatcaa agaagtctta acttgactcc atttactgct actgggaatt
     1381 tcagagagat gatagataat gttataatct ttattccatt tggcttgctt ttgaatgtca
     1441 attttaaaga aatcggattt ttacctaagt ttgcttttgt actggtttta agtcttactt
     1501 ttgaaataat tcaatttatc ttcgctattg gagcgacaga cataacagat gtaattacaa
     1561 atactgttgg aggctttctt ggactgaaat tatatggttt aagcaataag catatgaatc
     1621 aaaaaaaatt agacagagtt attatttttg taggtatact tttgctcgta ttattgctcg
     1681 tttaccgtac ccatttaaga ataaattacg tgtaagatgt ctaaatcaag caatctgatc
     1741 tttcatacac ataaagatat tgaatgaatt ggattagatg gaaaacggga tgtggggaaa
     1801 ctcgcccgta ggtgtgaagt gaggggaaaa ccggtgataa agtaaaaagc ttacctaaca
     1861 ctatagtaac aaagaaagcc caattatcaa ttttagtgct gaggaattgg tctctttaat
     1921 aaatttcctt aacgttgtaa atccgcattt tcctgacggt acccccaaac taagatttca
     1981 tagatagatt ttctgataac tttaaaaaac acgaatagcg gaaaggatgt cttcattcgc
     2041 taaagagtaa taaactactt ttccatcttg acgtgatttt gccattccat gtttttttaa
     2101 aaaacgtaaa tggtgtgacg ttgtagcgac actagcagta agtatttctg ctaaatcaca
     2161 gacgcacatt tccttatagg tttctaaagc atagaaaatt ttaattctag aaaaatcgct
     2221 gaaacatttc cctaacacaa gtaagtttga aaaatctttt tgatccaatt tattttttat
     2281 tccatttact ttttctttat gtacgacagt cacttggcaa acttttttca ttcatctcac
     2341 ctcacattca aacgattatt tgaatgaagt atacctattt gatcactctt ttgtcaatct
     2401 accttaattc caatacacta ttataagcaa tttcatgtat tgaagtaaac ttacttgcaa
     2461 gtgcgttttt gattccaagt taaaaacata ttattattct acataatttc ctaatcaaat
     2521 aaaactcctt tgaaaattgt tctaaaggag ttttattatt ttgtatttct actaaaaata
     2581 gcgtacaatg taattactaa gtaattacga aatgtaaaat aatggaggaa ataatatgat
     2641 tacaaaaaca agaaaacaag gaaattctat tatgttaacg gtccctaaag aattcgatgt
     2701 gcccaatggt gttgaagtag aagctaagct tgtagagaat ggtatccttt atgagtttgt
     2761 agagcctaaa aaagaatttt ttgattttag tgaagatatt ttggctgata ttctttcaga
     2821 aggttatagc aaacaagaga tcttaaaaga atttagaaat agaaaaaatg cgttaacttc
     2881 tgcatttaaa tctattgctg aagatactgt tgcaaattct aaaccgatga caaaagagga
     2941 gttggcagca gaaattggct tatgagattc taccttctaa acacgtaata aagtatctaa
     3001 aaaaattaaa agagaaaaca ttaaaagaac aatttttaac gataatttat gatgagatag
     3061 cagttcgtcc tcattcggga gaacaaaaaa cgggtgattt atcaggaatt tgggctattg
     3121 gattcaagta tgcaggtacg acatatcgcg ttgcatatga aattaaagat aatgcagtga
     3181 tacctatctt actatgtgga acgcatgaaa atttttatga acaattaaaa aagattaggt
     3241 aagaacccaa tgtttcaact tgagaggata gacgacactt taaaagataa tttttaacag
     3301 ctgagagttg taatacccta taattttaaa ccactaacct atatatttat gaaaaataga
     3361 aaaaattaaa acgcatttta agcctctttc aaattaaaag cttcatcctt atgcacaaag
     3421 agggcttaaa atgcgttatt ttatattgtt ttttagtaat aataaaaaaa tcactctaat
     3481 tctcccataa atattataga ttcctaaaga ttaaaataga ttattcccta ttgtctttta
     3541 agtagcggta gagtgttgcc ttgctaatac cagtcatttc cgtaatttct tttacgctat
     3601 aaacctgact tttatacaat tttactgctc gatcaacatc tttctggttc actcgtggac
     3661 ggccaccttt acgacctctc gcacgtgcac ttttcaaacc ttctttggta cgttcaacaa
     3721 ttaaatcacg ttcgaactga ctaaatgctt ggaagacagt catcattaag cgtccgtgag
     3781 gcgtgctggt atcgaagttt tctttcaagc tcaccagctt aacatggtgt tcttcaaaat
     3841 aggtaacaag gtcaatcaaa tcctttgtac tacgacccaa tcgagaaaaa ctttctacaa
     3901 ttaccgtatc tcccggacga attttatctt ttaaacgatt tagttgggga cgaaccgctt
     3961 tggttcccgt cattttctca gttaggattt cattacaatt ttgttcattg agtaagtcta
     4021 attgacgatt tagttcttgt gaacgtgtac tcactcgggc ataaccataa atgtatccac
     4081 tcatgctttt cttcctttcg tactttaatt ctggcttgat tgtatcataa aagggagtta
     4141 tagatacata gttaatgcaa caagttatga tacaaaatat gacctctttt atttaataaa
     4201 agaggtcatt tcattttgtc tcataatcga ttgtttttga tacgagtgtg tatacgaaat
     4261 aaacaaaaaa atcttaagta aatttctaga tacctaattt aatcctaact agtgtatcct
     4321 aaagttaata acgagtttat cacaatgaaa attataaaat gctcccatat tagatataac
     4381 atgcaatatt tgaattagaa ctacaaaatt tattcgtttt ataaagtaaa taatgttgag
     4441 atactaagga atagaatatt ataaggaatt gagctgaatg tattggataa aaaaagaata
     4501 actttattag agatggacta taaaaatgct cgtaattttc ttctgaagtc tgaaagctat
     4561 ttgaatgctc agctaccaga atatattagt tttgacgaaa ctttaaaatc tgctaaaaaa
     4621 gttttaactt cgccgtttgt ataattaagc gagacaaata aaaaagc
//
LOCUS       DAEGIQ010000328         3112 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.202, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000328 DAEGIQ010000000
VERSION     DAEGIQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.202"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            245..1213
                     /locus_tag="KMT45_003159"
     CDS             245..1213
                     /locus_tag="KMT45_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBE7006517.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="KMT45_003160"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="KMT45_003160"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBE7006518.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="KMT45_003161"
     CDS             2243..2851
                     /locus_tag="KMT45_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBE7006519.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          918 a          602 c          756 g          836 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt at
//
LOCUS       DAEGIQ010000329         2833 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.208, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000329 DAEGIQ010000000
VERSION     DAEGIQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.208"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1431
                     /locus_tag="KMT45_003162"
     CDS             <1..1431
                     /locus_tag="KMT45_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE7006520.1"
                     /translation="FSQIVSEVLTQIQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDK
                     VNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNVDSFSYVSDIAQRLKEFFNFLDMKF
                     KQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKIL
                     IYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKK
                     GCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKD
                     GSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKF
                     LGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKN
                     INAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKD
                     KQWQRQVETNSRVKERLEQIIGSLTG"
     gene            1438..1815
                     /locus_tag="KMT45_003163"
     CDS             1438..1815
                     /locus_tag="KMT45_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE7006521.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1966..2748
                     /locus_tag="KMT45_003164"
     CDS             1966..2748
                     /locus_tag="KMT45_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HBE7006522.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT         1055 a          398 c          572 g          808 t
ORIGIN      
        1 ttctcacaaa tcgtgtccga ggtattgact caaatccaat ttatggagtt ttatgagaac
       61 ttttattttg atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg
      121 ccagatgata aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg
      181 aatacatatt ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa cgtggatagc
      241 ttttcctatg taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg
      301 aaatttaaac aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta
      361 agtgaactaa acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg
      421 gaaggactat ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta
      481 atttattctg aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc
      541 gtcctagagc aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact
      601 atgattgttc aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt
      661 ctattagagg acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag
      721 gagcatatag ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa
      781 gtttcagagg aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta
      841 aaacaagaaa catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat
      901 aaatcaggtg agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga
      961 atgattaaca acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca
     1021 gaaatgacaa gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa
     1081 tttcaggaaa aactggttac caataatgga gatgtgcttg atctagatga agataatgaa
     1141 gtcgatgatg tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat
     1201 ggttattgta gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat
     1261 tgcactcact tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt
     1321 acagaagagt tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat
     1381 agccgtgtta aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg
     1441 gataaacaag ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag
     1501 acaagggaaa aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat
     1561 tttaattcaa tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat
     1621 attaggcaaa gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa
     1681 cccaagaaaa gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg
     1741 gaattagaaa aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg
     1801 tataataaag aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta
     1861 acggtcagct ttattgaaca gtaatttaag tatatgtcca atctagggta agtaaattga
     1921 gtatcaatat aaactttata tgaacataat caacgaggtg aaatcatgag caatttgatt
     1981 aacggaaaaa taccaaatca agcgattcaa acattaaaaa tcgtaaaaga tttatttgga
     2041 agttcaatag ttggagtata tctatttggt tcagcagtaa atggtggttt acgcattaac
     2101 agcgatgtag atgttctagt cgtcgtgaat catagtttac ctcaattaac tcgaaaaaaa
     2161 ctaacagaaa gactaatgac tatatcagga aagattggaa atacggattc tgttagacca
     2221 cttgaagtta cggttataaa taggagtgaa gttgtccctt ggcaatatcc tccaaaaaga
     2281 gaatttatat acggtgagtg gctcaggggt gaatttgaga atggacaaat tcaggaacca
     2341 agctatgatc ctgatttggc tattgtttta gcacaagcaa gaaagaatag tatttctcta
     2401 tttggtcctg attcttcaag tatacttgtc tccgtacctt tgacagatat tcgaagagca
     2461 attaaggatt ctttgccaga actaattgag gggataaaag gtgatgagcg taatgtaatt
     2521 ttaaccctag ctcgaatgtg gcaaacagtg actactggtg aaattacctc gaaagatgtc
     2581 gctgcagaat gggctatacc tcttttacct aaagagcatg taactttact ggatatagct
     2641 agaaaaggct atcggggaga gtgtgatgat aagtgggaag gactatattc aaaggtgaaa
     2701 gcactcgtta agtatatgaa aaattctata gaaacttctc tcaattaggc taattttatt
     2761 gcaataacag gtgcttactt ttaaaactac tgatttattg ataaatattg aacaattttt
     2821 gggaagaata aag
//
LOCUS       DAEGIQ010000330         2193 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.228, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000330 DAEGIQ010000000
VERSION     DAEGIQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.228"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            310..1005
                     /gene="vanR"
                     /locus_tag="KMT45_003165"
     CDS             310..1005
                     /gene="vanR"
                     /locus_tag="KMT45_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBE7006523.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            983..2137
                     /locus_tag="KMT45_003166"
     CDS             983..2137
                     /locus_tag="KMT45_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBE7006524.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          735 a          405 c          466 g          587 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccgaaa ttactaatgt atctagggct
       61 tcattataca ggaaattatc agaagtgaat aattagccat tctgtattcc gctaatgggc
      121 aatattttta aagaagaaaa ggaaactata aaatattaac agcctcctag cgatgccgaa
      181 aagccctttg ataaaaaaag aatcatcatc ttaagaaatt cttagtcatt tattatgtaa
      241 atgcttataa attcggccct ataatctgat aaattattaa gggcaaactt atgtgaaagg
      301 gtgataacta tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg
      361 gttgaattat acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa
      421 gcattggaat gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc
      481 ggcacaagcg gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc
      541 atgctgaccg ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat
      601 gattatataa cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg
      661 cgccgataca aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc
      721 ggccttgtca ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt
      781 actcccaccg agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc
      841 tccgagctgc tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc
      901 atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa
      961 tatataaaaa cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc
     1021 caaactagaa cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt
     1081 cgtgttgtat attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt
     1141 ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg
     1201 gaacaatata gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg
     1261 cgtcatgctt tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact
     1321 tattcagaac gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa
     1381 gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca
     1441 aagaaaaaat gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat
     1501 tatcggttat ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa
     1561 gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt
     1621 tgagattaca cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata
     1681 ctatatgctg gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca
     1741 ggcggttatt cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag
     1801 agtctttaac aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga
     1861 cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat
     1921 cccaaaagat aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc
     1981 ttccgatacg ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca
     2041 tggagggcag atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc
     2101 agcgatgcca gacttggttg ataaaaggag gtcctaagag atgtatataa ttttggctct
     2161 ttgtcaaatg atgttggtaa agagaaatct tag
//
LOCUS       DAEGIQ010000331         2095 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.231, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000331 DAEGIQ010000000
VERSION     DAEGIQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.231"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(154..816)
                     /locus_tag="KMT45_003167"
     CDS             complement(154..816)
                     /locus_tag="KMT45_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBE7006525.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            1208..1267
                     /locus_tag="KMT45_003168"
     CDS             1208..1267
                     /locus_tag="KMT45_003168"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HBE7006526.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            1325..2056
                     /gene="erm(A)"
                     /locus_tag="KMT45_003169"
     CDS             1325..2056
                     /gene="erm(A)"
                     /locus_tag="KMT45_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HBE7006527.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVIAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT          743 a          368 c          309 g          675 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ttaatagtaa cataagatac
       61 aggctgattt tttattcatt gttatttata ctaaagcacc cgatagctct gaaaacaatc
      121 acaaattcaa cttttcaaag ccacagcttt aagttatttt gtccagacaa cccccattgc
      181 ccaacccatt ttatggaatt ggcatccagg caacaacttt tcatataaat cgtaataatt
      241 ttgttcagat aggtacttat ctgaagctaa atgctcaagc catgatttag atgtgttgtg
      301 attataaatt ctaatcgcat tttttattcc aaatttgaaa caatgcggta aaaactcttg
      361 gattgcccct aatttataaa cataggtagg cggtgtctcg acttcagata cattatcaag
      421 aataactatt cttccttcct cattcaacag ttccttcatt tgctgtatta cgctggctat
      481 atcatccaaa tgatgaaagg ttgttcggct tacaataaaa tcaaacttct cattaaaatt
      541 aagttgttct gcattcatat tcagatagac cgtatttgtt agttgacgtt tagatttggc
      601 aagatcgagc atttgattag aaatatcaat ccctaccact tcatcataat aacttgctaa
      661 tttctccact aacaaacccg agccacatcc gatatctaat gctctgcctt tctttggaga
      721 catattagac acaaagaatg aataatcatt caaaagctca ttcacgaaat cgtaatcttc
      781 tgcaacctta tcaaactgtg attctattgt attcaaaaag atcccccatt cctactttat
      841 cgacattctt tcattactta ccactttaga tgttttttcg ttggggataa aacttccctt
      901 tagacaattt tatccaaaga caatacaaca gtgcaacttt attaaagtca ctgtccttta
      961 tcgcagcctt tactttttag taaagacagt ggcttctctt atcaagtttc aaaacatatt
     1021 attttgaaga aaacgtccat ctgaagtgtc aagtgcaaaa ttacatataa aggtttattc
     1081 taaaatgaaa agatgataca atcatattca gttacataag gaggtttcaa ttatgtgcac
     1141 cagtatcgca gtagtagaaa ttactttatc tcattcataa tgaaaaaaat ggaaaggaga
     1201 taaaagtatg ggtacttttt ctatatttgt tattaataaa gttcgttatc aaccaaatca
     1261 aaattaattg gttataatga acgcttaatg tcagttcatt ataaccagta aggagaaggt
     1321 tataatgaac cagaaaaacc ctaaagacac gcaaaatttt attacttcta aaaagcatgt
     1381 aaaagaaata ttgaatcaca cgaatatcag taaacaagac aacgtaatag aaatcggatc
     1441 aggaaaagga cattttacca aagagctagt caaaatgagt cgatcagtta ttgctataga
     1501 aattgatgga ggcttatgtc aagtgactaa agaagcggta aacccctctg agaatataaa
     1561 agtgattcaa acggatattc taaaattttc cttcccaaaa catataaact ataagatata
     1621 tggtaatatt ccttataaca tcagtacgga tattgtcaaa agaattacct ttgaaagtca
     1681 ggctaaatat agctatctta tcgttgagaa gggatttgcg aaaagattgc aaaatctgca
     1741 acgagctttg ggtttactat taatggtgga gatggatata aaaatgctca aaaaagtacc
     1801 accactatat tttcatccta agccaagtgt agactctgta ttgattgttc ttgaacgaca
     1861 tcaaccattg atttcaaaga aggactacaa aaagtatcga tcttttgttt ataagtgggt
     1921 aaaccgtgaa tatcgtgttc ttttcactaa aaaccaattc cgacaggctt tgaagcatgc
     1981 aaatgtcact aatattaata aactatcgaa ggaacaattt ctttctattt tcaatagtta
     2041 caaattgttt cactaaatta aagtaataaa gcgttctcta atttcacaag aggac
//
LOCUS       DAEGIQ010000332         1504 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.253, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000332 DAEGIQ010000000
VERSION     DAEGIQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.253"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            262..345
                     /locus_tag="KMT45_003170"
                     /pseudo
     CDS             262..345
                     /locus_tag="KMT45_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="KMT45_003171"
     CDS             394..477
                     /locus_tag="KMT45_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBE7006528.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="KMT45_003172"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="KMT45_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBE7006529.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAEGIQ010000333         1349 bp    DNA     linear   BCT 28-MAY-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00014828
            SAMN18720224-rid12297683.guided.263, whole genome shotgun sequence.
ACCESSION   DAEGIQ010000333 DAEGIQ010000000
VERSION     DAEGIQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18720224
            Sequence Read Archive: SRR14673409
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001049062.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001049062.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/28/2021 15:56:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,174
            CDSs (total)                      :: 3,114
            Genes (coding)                    :: 3,036
            CDSs (with protein)               :: 3,036
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 3 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 33 of 78
            Pseudo Genes (incomplete)         :: 33 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 10 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18720224-rid12297683.guided.263"
                     /strain="AUSMDU00014828"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            46..297
                     /locus_tag="KMT45_003173"
                     /pseudo
     CDS             46..297
                     /locus_tag="KMT45_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="KMT45_003174"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="KMT45_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBE7006530.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
